UNASSIGNED: Cell-on-a-chip systems have become promising devices to study the effectiveness of new anticancer drugs recently. Several microdevices for @entity96 culture and evaluation of the drug @entity137 have been reported. However, there are still no proven reports about high-throughput and simple methods for the evaluation of drug @entity137 on @entity96 cells. The paper presents the results of the effects of the anticancer drug ( @entity1487 , @entity1487 ) on the HepG2 spheroids as a model of @entity96 . The experiments were based on the long-term 3D spheroid culture in the microfluidic system and monitoring of the effect of @entity1487 at two selected concentrations (0.5 mM and 1.0 mM). Our investigations have shown that the initial size of the spheroids has influence on the drug effect. With the increase of the spheroids diameter, the drug resistance (for the two tested @entity1487 concentrations) decreases. This phenomenon was observed both through cells metabolism analysis, as well as changes in spheroids sizes. In our research, we have shown that the lower @entity1487 (0.5 mM) concentration causes higher decrease in HepG2 spheroids viability. Moreover, due to the microsystem construction, we observe the drug resistance effect (10(th) day of culture) regardless of the initial size of the created spheroids and the drug concentration. This article is protected by copyright. All rights reserved.

Studies of anticancer drug XXXX based on long-term HepG2 spheroid culture in a microfluidic system.

@entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity1487 :: ('MESH:D005472', 'Chemical') :: ['5-FU', '5-fluorouracil'] @entity96 :: ('MESH:D008113', 'Disease') :: ['liver cancer']
@entity137 :: (MESH:D064420,Disease) :: ['cytotoxicity']
