Large-conductance, voltage- and @entity460 -activated @entity972 ( @entity4030 ) channels play a key role in cell excitability. @entity4030 channels are composed of a pore-forming alpha-subunit and a regulatory beta-subunit, of which four (beta1-4) genes have been identified. Previous findings suggested that @entity4030 channel activity is regulated by @entity53 . However, the underlying mechanisms have remained incompletely documented. Therefore, we used reverse transcriptase polymerase chain reaction to clone four cDNA fragments that were specific to the @entity840 alpha, beta1, beta2, and beta4 genes. Using a sensitive ribonuclease protection assay, we found that the alpha and beta4 mRNAs were the most abundant mRNAs in the brain and pituitary, whereas in the aorta, the alpha-subunit was coexpressed with the beta1-subunit. Moreover, there was a significant upregulation of the alpha- but not the beta1-subunit mRNA and the alpha-subunit protein in the aorta of the estrogenvs oil-treated ovariectomized animals. In specific brain areas including preoptic area, ventral hypothalamus, hippocampus, and amygdala, and in the pituitary, neither the alpha- nor beta4-subunit mRNAs were affected by @entity172 . These findings suggest that @entity172 may not affect the mRNA expression of @entity4030 channels in the brain and pituitary. However, @entity172 causes increased expression of @entity4030 alpha in the aorta, which may explain some of the cardioprotective effects of @entity172 in @entity1 .

Effect of XXXX on mRNA expression of large- conductance, voltage-dependent, and @entity460 -activated @entity972 channel alpha and beta subunits in @entity840 .

@entity172 :: ('MESH:D004967', 'Chemical') :: ['estrogen'] @entity1 :: ('9606', 'Species') :: ['women'] @entity840 :: ('10141', 'Species') :: ['guinea pig'] @entity4030 :: ('3778', 'Gene') :: ['MaxiK'] @entity53 :: ('MESH:D004958', 'Chemical') :: ['estradiol'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity972 :: ('MESH:D011188', 'Chemical') :: ['potassium']
@entity53 :: (MESH:D004958,Chemical) :: ['17beta-estradiol']
