@entity1550 ; @entity1550 ), a protease originally identified in @entity0 , is characterized by a potent degrading activity against a wide spectrum of extracellular matrix proteins. The aims of this study were to localize and identify the @entity1550 -expressing cells in invasive @entity1 @entity0 and to evaluate the role of @entity1550 in transition to @entity3 by studying @entity3821 ( @entity3821 ). We found expression of @entity1550 in stromal fibroblast-like cells in all 21 invasive @entity513 studied and in 4 of 9 invasive @entity1137 . In most @entity189 , expression of @entity1550 was limited to small stromal foci in the @entity5 area. Combined in situ hybridization and immunohistochemistry showed coexpression of alpha-smooth muscle actin immunoreactivity and @entity1550 mRNA in myofibroblasts. In contrast, cytokeratin-positive @entity5 cells, alpha-smooth muscle actin-positive vascular smooth muscle cells, @entity469 -positive macrophages, and @entity2279 -positive endothelial cells were all @entity1550 mRNA negative. In situ hybridization for @entity1550 in 17 @entity3821 revealed expression in 10 cases. Immunohistochemical analysis of all @entity3821 cases identified microinvasion in 8 of the 17 lesions. Seven of the eight lesions with microinvasion were @entity1550 positive. Further analysis showed that @entity1550 expression was often associated with the microinvasive events. This particular expression pattern was unique for @entity1550 among other MMPs analyzed, including @entity70504 . We conclude that @entity1550 is primarily expressed by myofibroblasts in @entity1 @entity0 and that expression in @entity3821 often is associated with microinvasive events. On the basis of these data, we propose that @entity1550 may play an essential role during transition of @entity3821 to invasive @entity513 .

Collagenase-3 expression in breast myofibroblasts as a molecular marker of transition of XXXX lesions to invasive @entity513 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity1137 :: ('MESH:D018275', 'Disease') :: ['lobular carcinomas'] @entity3821 :: ('MESH:D002285', 'Disease') :: ['DCIS lesions', 'ductal carcinoma in situ', 'DCIS'] @entity70504 :: ('4313;4320', 'Gene') :: ['MMP-2, -11, and -14'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast carcinoma'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity469 :: ('968', 'Gene') :: ['CD68'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinomas'] @entity2279 :: ('5175', 'Gene') :: ['CD31'] @entity1550 :: ('4322', 'Gene') :: ['MMP-13', 'Collagenase-3 (matrix metalloproteinase 13'] @entity513 :: ('MESH:D044584', 'Disease') :: ['ductal carcinomas'] @entity3 :: ('MESH:D009362', 'Disease') :: ['invasive lesions']
@entity3821 :: (MESH:D002285,Disease) :: ['ductal carcinoma in situ']
