BACKGROUND AND OBJECTIVE: Skin aging results in physiological alterations in keratinocyte activities and epidermal function, as well as dermal changes. Yet, the cellular and molecular mechanisms that cause @entity409 during skin aging are not well understood. Recently, the role of epidermal hyaluronan (HA) as an active regulator of dynamic cellular processes is getting attention and alterations in HA metabolism are thought to be important in age-related @entity409 . Microneedle fractional radiofrequency (RF) has shown effects for improving cutaneous aging. However, little is known about the effects of fractional RF on the epidermal HA and epidermal function. We investigated the effect of microneedle fractional RF on the expression of epidermal HA in young and aged @entity19 epidermis. MATERIALS AND METHODS: We performed fractional RF on the dorsal skin of 30 8-week-old (young) hairless @entity19 and 15 47-week-old (aged) C57BL/6J @entity19 . Skin samples were collected on day 1, 3, and 7. HA content was measured by ELISA. Gene expressions of @entity3844 , @entity68528 , and @entity14659 were measured using real time RT-PCR. Immunohistochemistry for detection of HA, @entity3844 , @entity4698 , and filaggrin were performed. RESULTS: HA content and the mRNA levels of @entity68528 , @entity3844 , and @entity14659 were upregulated in the epidermis of both young and aged @entity19 after microneedle fractional RF treatment. The expression was increased from day 1 after treatment and increased expression persisted on day 7. Fractional RF treatment significantly increased @entity4698 and filaggrin expression only in the aged @entity19 skin. CONCLUSION: Microneedle fractional RF increased epidermal HA and @entity3844 expression in both young and aged @entity19 and reversed age-related @entity409 especially in aged @entity19 , suggesting a new mechanism involved in the skin rejuvenation effect of microneedle fractional RF. Lasers Surg. Med. 48:140-149, 2016.    2015 Wiley Periodicals, Inc.

Microneedle fractional radiofrequency increases epidermal hyaluronan and reverses age-related XXXX .

@entity19 :: ('10090', 'Species') :: ['mice'] @entity409 :: ('MESH:D008107', 'Disease') :: ['epidermal dysfunction'] @entity14659 :: ('15118(Tax:10090)', 'Gene') :: ['HAS3'] @entity4698 :: ('18538(Tax:10090)', 'Gene') :: ['PCNA'] @entity3844 :: ('12505(Tax:10090)', 'Gene') :: ['CD44', 'CD 44'] @entity68528 :: ('56541(Tax:10090)', 'Gene') :: ['HABP4']
@entity409 :: (MESH:D008107,Disease) :: ['epidermal dysfunction']
