BACKGROUND: The role of monocytes and neutrophils is crucial during acute allograft rejection. They have the capacity to generate toxic reactive @entity26 intermediates in response to specific agonists that may act as tissue destructive molecules. We examined the possibility of reactive intermediate-mediated tissue injury in acute lung allograft rejection, as well as the effect of @entity224 dismutase. METHODS: Allogenic (Brown Norway to F344) or syngenic (F344 to F344) @entity35 left-lung transplantation was performed. Generation of reactive @entity26 intermediates in peripheral blood was evaluated by the method of @entity6931 -dependent chemiluminescence. Cell membrane phospholipid peroxidation in the graft was measured as @entity1234 concentration. The third group of animals having allografts received @entity291 erythrocyte @entity224 dismutase (5,000 U/kg intravenously every 12 hours after transplantation). RESULTS: Relative chemiluminescence response in the allograft recipient to normal F344 was elevated on postoperative day 1 (257%), then decreased slightly on day 3 (156%) and was elevated again on day 7 (560%) as the process of rejection progressed. Allograft tissue @entity1234 levels (248.37 +/- 112.35 nM/whole lung, n = 6; p < 0.05 by Student's t test) were higher than isograft levels (139.29 +/- 35.93 nM/whole lung, n = 6) on day 7. @entity224 dismutase treatment significantly ameliorated the histologic degree of rejection on day 7. CONCLUSIONS: These results demonstrate the tissue destructive activity of reactive @entity26 intermediates during lung allograft rejection. To scavenge free radicals may be a useful therapeutic modality in the management of acute lung allograft rejection.

Free radical-mediated tissue injury in acute lung allograft rejection and the effect of XXXX dismutase.

@entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity6931 :: ('MESH:D008165', 'Chemical') :: ['luminol'] @entity1234 :: ('MESH:D008315', 'Chemical') :: ['malondialdehyde'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity224 :: ('MESH:D013481', 'Chemical') :: ['superoxide', 'Superoxide'] @entity291 :: ('9913', 'Species') :: ['bovine']
@entity224 :: (MESH:D013481,Chemical) :: ['superoxide']
