The influence of @entity13204 (EP) on eosinophil survival was examined by an in vitro cell culture technique. Nasal epithelial cells (NECs) were stimulated with 25 ng/ml @entity310 in the presence of EP (10 to 30 ng/ml). After 24 h, the culture supernatants were obtained and used as conditioned media of NECs (CM). Eosinophils (1 x 10(3) cells/ml) prepared from healthy @entity1 peripheral blood were incubated with 25% CM and eosinophil survival was assessed by @entity3460 dye exclusion test 48 h later. CM prepared from NEC cultures pre-treated with @entity310 and EP caused a decrease in eosinophil survival as compared with that from NEC cells pre-treated with @entity310 alone. The minimum concentration of EP that caused a significant decrease in eosinophil survival was 25 ng/ml. The addition of EP into eosinophil cultures did not cause inhibition of eosinophil survival, which was prolonged by stimulation with @entity2841 ( @entity2841 ), even when 40 ng/ml EP was added to cell cultures. We then examined the levels of @entity2841 in CM by ELISA. Treatment of NECs with EP at more than 25 ng/ml, reduced the ability of NECs to produce @entity2841 in response to @entity310 stimulation. These results may suggest that EP suppresses eosinophil survival through the suppression of @entity2841 production from NECs induced by inflammatory stimulation and that this suppressive effect contributes, in part, to the therapeutic mode of action of EP on @entity56 .

Modulation of eosinophil survival by XXXX , an H1 receptor antagonist, in vitro.

@entity1 :: ('9606', 'Species') :: ['human'] @entity310 :: ('7124', 'Gene') :: ['TNF-alpha'] @entity13204 :: ('MESH:C053090', 'Chemical') :: ['epinastine hydrochloride'] @entity3460 :: ('MESH:D014343', 'Chemical') :: ['trypan blue'] @entity56 :: ('MESH:D004342', 'Disease') :: ['allergic diseases'] @entity2841 :: ('1437', 'Gene') :: ['granulocyte-macrophage colony-stimulating factor', 'GM-CSF']
@entity13204 :: (MESH:C053090,Chemical) :: ['epinastine hydrochloride']
