BACKGROUND: Collagen is a part of structural connective tissue in every organ of the body. Study of collagen brings into focus the current knowledge of biosynthesis and degradation as a complex mechanism. One of the major aspects of @entity5 cell invasion and @entity3 is the interaction between @entity5 cells and extracellular matrix component. Property of picrosirius red to enhance the birefringence is the result of basic protein nature of collagen molecules. There exists a direct relationship between the presence of type IV collagen and degree of differentiation in @entity957 ( @entity957 ) cells as they lose their capability to form the basement membrane. MATERIALS AND METHODS: Thirty cases of oral @entity957 (OSCC) were evaluated for standard staining protocol of picrosirius red to analyze collagen fibers in different grades of OSCC under polarizing microscopy. RESULTS: In this study, moderately to poorly differentiated OSCC cases showed a gradual change in polarizing colors from yellowish orange to greenish yellow particularly in the vicinity of invading @entity5 islands. Thick collagen fibers forming bundles in parallel disposition were found around the neoplastic areas in discontinuous fashion. Type I collagen fibers of different lengths were strongly birefringent and swirl pattern, parallel orientation was evident. CONCLUSION: An observable stromal change with the progression of @entity5 was evinced with picrosirius red stain in different thickness of collagens with a significant change in the arrangement from the early stage to the advanced stage according to tumorogenesis.

Histopathological evaluation of collagen fibers using picrosirius red stain and polarizing microscopy in XXXX .

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'neoplasm'] @entity957 :: ('MESH:D002294', 'Disease') :: ['squamous cell carcinoma', 'SCC'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis']
@entity957 :: (MESH:D002294,Disease) :: ['oral squamous cell carcinoma']
