The @entity1919 ( @entity268 ) pathway represents a central signaling cascade with crucial functions in all organisms. Previous studies of @entity8869 (anamorph of @entity8869 ) suggested a function of @entity268 signaling in regulation of cellulase gene expression. We were therefore interested in how the crucial components of this pathway, adenylate cyclase (ACY1) and @entity268 -dependent protein kinase A (PKA), would affect cellulase gene expression. We found that both ACY1 and PKA catalytic subunit 1 (PKAC1) are involved in regulation of vegetative growth but are not essential for sexual development. Interestingly, our results showed considerably increased transcript abundance of cellulase genes in darkness compared to light (light responsiveness) upon growth on @entity1132 . This effect is strongly enhanced in mutant strains lacking PKAC1 or ACY1. Comparison to the wild type showed that ACY1 has a consistently positive effect on cellulase gene expression in light and darkness, while PKAC1 influences transcript levels of cellulase genes positively in light but negatively in darkness. A function of PKAC1 in light-modulated cellulase gene regulation is also reflected by altered complex formation within the cel6a/cbh2 promoter in light and darkness and in the absence of pkac1. Analysis of transcript levels of cellulase regulator genes indicates that the regulatory output of the @entity268 pathway may be established via adjustment of XYR1 abundance. Consequently, both adenylate cyclase and protein kinase A are involved in light-modulated cellulase gene expression in @entity8869 and have a dampening effect on the light responsiveness of this process.

Roles of protein kinase A and adenylate cyclase in light-modulated cellulase regulation in XXXX .

@entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity1919 :: ('MESH:D000242', 'Chemical') :: ['cyclic AMP'] @entity8869 :: ('51453', 'Species') :: ['Hypocrea jecorina', 'Trichoderma reesei', 'T. reesei'] @entity1132 :: ('MESH:D007785', 'Chemical') :: ['lactose']
@entity8869 :: (51453,Species) :: ['Trichoderma reesei']
