UNASSIGNED: Acute @entity3145 is accompanied by synthesis of @entity842 (NO) in the inflamed tissue as well as in the spinal cord. NO release at the spinal level is likely involved in the neuroplastic changes contributing to @entity158 . Also, previous studies indicate that this could be due to the inducible isoform of the @entity842 synthase ( @entity1506 ) enzyme. Though, the role of NO has been investigated in several animal models of nociception, the precise contribution of NO to nociception arising from hind paw incision is unknown, which is a rodent model of @entity282 . In the present work, we have estimated the formation of NO in @entity35 , both at the site of incision and the corresponding spinal cord levels by Griess assay. Subsequently, naive @entity35 were implanted with chronic indwelling intrathecal (i.t.) catheters. Fixed quantity (30   g) of 1400  W, an @entity1506 inhibitor, was either administered locally into the wound at the time of incision or into the i.t. space, 15  min before hind paw incision. In a different set, i.t. 1400  W was administered, 20  h after incision. Control group received i.t. saline. Nociception was evaluated by guarding score, mechanical @entity1931 and thermal @entity1931 . NO level was significantly increased between 4  h - day 1 locally and at 4  h at the spinal level after incision. Local inhibition of @entity1506 produced transient decrease of guarding (4-12  h) whereas pronounced decrease of guarding and @entity1931 was evident after spinal inhibition of @entity1506 . Also, spinal NO level decreased after i.t. drug administration. Post-incision drug treatment resulted in greater antinociceptive effect at day 1 though not on day 2. These results indicate involvement of NO in postincisional nociception in @entity35 .

Antinociceptive effect of 1400  W, an inhibitor of inducible XXXX synthase, following hind paw incision in @entity35 .

@entity1931 :: ('MESH:D006930', 'Disease') :: ['allodynia', 'hyperalgesia'] @entity1506 :: ('24599(Tax:10116)', 'Gene') :: ['iNOS'] @entity3145 :: ('MESH:D017695', 'Disease') :: ['tissue damage'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity158 :: ('MESH:D010146', 'Disease') :: ['pain'] @entity35 :: ('10116', 'Species') :: ['Sprague-Dawley rats', 'rats'] @entity282 :: ('MESH:D010149', 'Disease') :: ['postoperative pain']
@entity842 :: (MESH:D009569,Chemical) :: ['nitric oxide']
