@entity3089 ( @entity3090 ) is a potent polyphenolic antioxidant extracted from green tea. Due to its antimutagenic and antitumor activities, it is a promising candidate for use in topical formulations for @entity1489 prevention. The overall goal of this study was therefore to determine the influence of several factors on the stability of @entity3090 in solution to obtain information that would facilitate the subsequent development of topical formulations. Our first objective was to determine the influence of pH, temperature, and ionic strength on the aqueous stability of @entity3090 . A second objective was to determine the stability of @entity3090 in various solvents in the presence and absence of different antioxidants. A simple and rapid stability indicating high-performance liquid chromatography assay for @entity3090 was developed. Stability studies were performed in 0.05 M aqueous buffers at pH 3, 5, 7, and 9 at 4, 25, and 50 degrees C. The effect of ionic strength on @entity3090 stability was evaluated in 0.05 M acetate buffer, pH 5, adjusted to the desired ionic strength with @entity2827 . An accelerated stability study of @entity3090 was performed at 50 degrees C in the organic solvents @entity653 and @entity35018 in the presence of antioxidants. The degradation of @entity3090 increased rapidly as temperature and solution pH were increased. Ionic strength increases also caused an accelerated degradation. The solution stability of @entity3090 was prolonged in @entity653 and @entity35018 compared with an aqueous environment. The addition of 0.1% concentrations of several antioxidants in combination with 0.025% @entity1098 caused variable effects on @entity3090 stability. @entity1882 in @entity653 produced the greatest stability improvement for @entity3090 . The t(90) (time for 10% degradation to occur) was 76.1 days at 50 degrees C. It can be concluded that @entity653 -based vehicles are suitable for stabilizing @entity3090 .

Preformulation study of @entity3089 , a promising antioxidant for topical XXXX prevention.

@entity1489 :: ('MESH:D012878', 'Disease') :: ['skin cancer'] @entity3089 :: ('MESH:C045651', 'Chemical') :: ['Epigallocatechin gallate'] @entity653 :: ('MESH:D005990', 'Chemical') :: ['glycerin'] @entity3090 :: ('CHEBI:4806', 'Chemical') :: ['EGCG'] @entity1882 :: ('MESH:D002084', 'Chemical') :: ['Butylated hydroxytoluene'] @entity35018 :: ('MESH:C010111', 'Chemical') :: ['Transcutol P'] @entity2827 :: ('MESH:D012965', 'Chemical') :: ['sodium chloride'] @entity1098 :: ('MESH:D004492', 'Chemical') :: ['EDTA']
@entity1489 :: (MESH:D012878,Disease) :: ['skin cancer']
