Expression of mRNA for the 5 @entity1943 receptors (sst1-5) was characterized by Northern blot and RT-PCR analysis in 20 @entity1653 and 9 @entity11 samples. sst1 mRNA was detectable by Northern blots of poly-A+ RNA in @entity1653 but not @entity11 . In contrast, sst2 mRNA was readily detected by Northern blots of total RNA as a major 2.3 kb transcript and 2 minor 4.3 kb and 8 kb transcripts in all @entity1653 and 6 out of 9 @entity11 . Quantitation of the 2.3 kb sst2 mRNA showed that 15 out of 20 @entity5 expressed 1.3- to 33-fold higher levels than control normal @entity1 brain. Mean sst2 mRNA for the 20 @entity1653 samples was 978% that of normal brain. Three @entity11 showed 7- to 14-fold higher sst2 mRNA than normal brain whereas the remaining samples displayed very low or undetectable levels. Immunocytochemistry of @entity1653 and @entity11 samples, with a sst2-specific antibody revealed immunoreactivity in @entity5 cells and peritumoral tissue, with prominent expression in blood vessels. mRNA for @entity27756 ,4,5 could not be detected by Northern blots in any of the @entity5 . RT-PCR analysis of @entity1653 and @entity11 revealed the following percent of @entity5 positive for a given sst mRNA: sst1 (86%), sst2 (100%), @entity27756 (60%), sst4 (58%), and sst5 (67%); 85% of @entity5 expressed 3 of the 5 subtypes. No correlation was found between the pattern of expression of sst mRNA and @entity5 type, location, and histology for either the @entity1653 or @entity11 . Our results show that @entity1653 and @entity11 are all positive for at least one sst subtype, the majority expressing multiple subtypes. sst2 is the most abundant isoform with a rich expression in both @entity5 and peritumoral tissue especially blood vessels.

Expression of XXXX receptor subtypes in @entity1 brain tumors.

@entity1 :: ('9606', 'Species') :: ['human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity27756 :: ('25992', 'Gene') :: ['sst3'] @entity11 :: ('MESH:D005910', 'Disease') :: ['gliomas', 'glioma'] @entity1653 :: ('MESH:D008579', 'Disease') :: ['meningioma', 'meningiomas'] @entity1943 :: ('MESH:D013004', 'Chemical') :: ['somatostatin']
@entity1943 :: (MESH:D013004,Chemical) :: ['somatostatin']
