BACKGROUND: Uncontrolled cell proliferation and suppressed apoptosis are the critical events transforming a normal cell to a cancerous one wherein the inflammatory microenvironment supports this oncogenic transformation. The process of @entity694 may be aggravated in chronic inflammatory conditions such as @entity31 where non-steroidal anti-inflammatory drugs (NSAIDs) may effectively prevent the cellular and molecular events. METHODS: Western blots and immunofluorescent analysis of DNA mismatch repair enzymes, cell cycle regulators and pro- and anti-apoptotic proteins were performed in dextran sulfate sodium (DSS)-induced @entity31 and 1,2-dimethyl benz(a)anthracene ( @entity18399 )-induced @entity694 . Also, apoptotic studies were done in isolated colonocytes using fluorescent staining and in paraffin sections using TUNEL assay. RESULTS: An upregulation of cell cycle regulators: @entity4910 / @entity8985 and cyclin E/cdk2 and anti-apoptotic @entity588 , along with the suppression of DNA repair enzymes: @entity6756 and @entity4311 ; @entity5 suppressors: @entity876 , @entity3544 and Rb and pro-apoptotic proteins: @entity589 and Bad were observed in the DSS, @entity18399 and DSS+ @entity18399 groups. @entity1364 ( @entity1364 ) was also overexpressed in these groups. The ultimate executioner of the apoptotic pathway; @entity522 , was suppressed in these groups. Apoptotic studies in colonocytes and paraffin sections revealed suppressed apoptosis in these groups. These effects were corrected with the administration of a second generation NSAID, @entity12698 along with the treatment of DSS and @entity18399 . CONCLUSION: The chemopreventive action of @entity12698 in @entity1960 mediated @entity694 may include the regulation of DNA mismatch repair enzymes, cell cycle check points, cell proliferation and apoptosis.

@entity12698 prevents @entity1960 associated XXXX : An upregulation of apoptosis.

@entity6756 :: ('4292', 'Gene') :: ['MLH1'] @entity1364 :: ('5111', 'Gene') :: ['PCNA', 'Proliferating cell nuclear antigen'] @entity589 :: ('581', 'Gene') :: ['Bax'] @entity588 :: ('596', 'Gene') :: ['Bcl-2'] @entity522 :: ('836', 'Gene') :: ['caspase-3'] @entity12698 :: ('MESH:C105934', 'Chemical') :: ['celecoxib'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumour'] @entity18399 :: ('MESH:D004111', 'Chemical') :: ['DMH'] @entity31 :: ('MESH:D003093', 'Disease') :: ['ulcerative colitis'] @entity8985 :: ('1019', 'Gene') :: ['cdk4'] @entity4910 :: ('595', 'Gene') :: ['cyclin D1'] @entity4311 :: ('4436', 'Gene') :: ['MSH2'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity3544 :: ('644914', 'Gene') :: ['p21'] @entity1960 :: ('MESH:D003092', 'Disease') :: ['colitis'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon carcinogenesis', 'colon cancer']
@entity694 :: (MESH:D003110,Disease) :: ['colon carcinogenesis']
