In the prespermatogenesis period, male germ cells (gonocytes) begin to reproliferate and move to the basement membrane of the seminiferous tubule. Although these two events-reproliferation and relocation-are important for establishment of spermatogenesis, they have not been greatly analyzed both in a mechanical and in an endocrine or paracrine aspect. In this study, the relationship between reproliferation and relocation of gonocytes was examined, using the @entity1014 analog @entity1603 ( @entity1603 ) labeling method and @entity1579 ( @entity1579 ). @entity1603 was injected into the fetuses [day 13.5 post coitus (dpc) to 18.5 dpc] and pups [day 0. 5 post partum (dpp) to 6.5 dpp] of C57BL/6J @entity19 . Two hours later, @entity1603 positive gonocytes were examined immunohistochemically and these data were analyzed. @entity1579 and LM observation was carried out as well. Gonocytes began to relocate on the basement membrane from 18.5 dpc (1.4%) while @entity1603 -labeled gonocytes were first detected on 1.5 dpp (13.6%). Relocated @entity1603 -negative gonocytes were recognized from 18.5 dpc (1.4%), and relocated @entity1603 -labeled gonocytes were recognized from 1.5 dpp (8.4%). On the other hand, non-relocated @entity1603 -labeled gonocytes were detected from 1.5 dpp (5.2%). Gonocyte relocation began 2 days earlier than reproliferation during the late fetal period. After birth, the two events occurred at random. These results indicate that the reproliferation of the gonocyte does not correlate with relocation. The two events may be regulated by different mechanisms.

Reproliferation and relocation of XXXX male germ cells (gonocytes) during prespermatogenesis.

@entity19 :: ('10090', 'Species') :: ['mice'] @entity1603 :: ('MESH:D001973', 'Chemical') :: ['BrdU', 'bromodeoxyuridine'] @entity1014 :: ('MESH:D013936', 'Chemical') :: ['thymidine'] @entity1579 :: ('MESH:D017096', 'Disease') :: ['transmission electron microscopy', 'TEM']
@entity19 :: (10090,Species) :: ['mouse']
