Transcriptional regulation of the gene for the myeloid @entity460 binding protein, @entity7966 , was investigated in @entity1 @entity1778 cell lines. The @entity7966 gene was not expressed in monoblastic ML-1 cells, promonocytic U-937 cells, or promyelocytic HL-60 cells. On the other hand, the gene was expressed in monocytic THP-1 cells and in the HL-60 cells treated with @entity3873 (VD3). The level of @entity7966 in VD3-treated HL-60 cells was two-fold higher than that in THP-1 cells. Among several known transcription factor binding motifs, nuclear protein(s) of VD3-treated HL-60 cells and THP-1 cells bound to the @entity6863 ( @entity6863 )-binding motif that was located in the upstream region of the @entity7966 gene (-81), as evidenced by the competitive gel mobility-shift assay. An antibody for @entity6863 super-shifted the nucleoprotein complex in THP-1 cells but not in the VD3-treated HL-60 cells, whereas an antibody for @entity9322 blocked the formation of the complex with the nuclear factor of the HL-60 cells but not with that of THP-1 cells. An anti- @entity29726 antibody had no effect on the complex in either cell. Thus, it was concluded that @entity69639 were able to bind to the @entity6863 motif, and that @entity6863 bound to the motif in THP-1 cells and @entity9322 bound to that in the VD3-treated HL-60 cells. Furthermore, to examine the transcriptional activity of the @entity6863 motif, we transfected several constructed luciferase reporter DNAs into HL-60 cells and THP-1 cells. The luciferase activity of the @entity6863 motif in HL-60 cells was increased by VD3 treatment. The @entity6863 motif in the @entity7966 gene was confirmed to function as a regulatory region in VD3-treated HL-60 cells and THP-1 cells by the assay. Since @entity9322 was also detected in VD3-untreated HL-60 cells by immunoblotting, VD3 activated @entity9322 to bind to the motif, probably through post-translational modification.

Transcriptional regulation by @entity69639 in the expression of the gene for the @entity7966 myeloid XXXX binding protein.

@entity1 :: ('9606', 'Species') :: ['human'] @entity29726 :: ('1052', 'Gene') :: ['C/EBP delta'] @entity69639 :: ('1050;1051', 'Gene') :: ['C/EBP alpha and -beta'] @entity7966 :: ('6280', 'Gene') :: ['MRP14'] @entity3873 :: ('MESH:D002117', 'Chemical') :: ['1,25-dihydroxyvitamin D3'] @entity1778 :: ('MESH:D007938', 'Disease') :: ['monocytic leukemia'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity9322 :: ('1051', 'Gene') :: ['C/EBP beta'] @entity6863 :: ('1050', 'Gene') :: ['C/EBP alpha', 'C/EBP', 'CCAAT/enhancer binding protein']
@entity460 :: (MESH:D002118,Chemical) :: ['calcium']
