STUDY DESIGN: Autologous nucleus pulposus obtained from coccygeal intervertebral discs was grafted on the proximal of L5 dorsal root ganglion. @entity158 behavior, mRNA expression of Interleukin-8 (IL-8), and immunohistochemical changes were assessed. OBJECTIVE: The purpose of this study is to investigate temporal changes of IL-8 expression in the spinal cord and dorsal root ganglion and the @entity158 with time course and to elucidate whether repertaxin (IL-8 receptor inhibitor) attenuates @entity158 in a @entity35 model of @entity890 . SUMMARY OF BACKGROUND DATA: Inflammatory mediators like cytokines and chemokines have been implicated in @entity158 because of disc herniation. IL-8, known as CXCL8, is a chemokine, which has been reported to be associated with @entity2531 and chronic @entity158 states. METHODS: Lumbar disc herniated @entity35 model was made by implantation of the autologous nucleus pulposus, harvested from the coccygeal vertebra of each tail, on the left L5 nerve root just proximal to the dorsal root ganglion. @entity35 were tested for mechanical @entity1931 and thermal @entity1931 at 2 days before surgery, and on days 1, 5, 10, 20, 30, 40, 50, and 60 postoperatively. Experimental group was intrathecally injected with the IL-8 receptor inhibitor at L5 level on postoperative day 10. Mechanical @entity1931 of the plantar surface of both hindpaw was tested on 30 minutes, 1, 3 hours, 1, 3, 5, and 10 days after administration. For the staining of astrocytes and microglia, immunohistochemical study was done 20 days after surgery. RESULTS: Mechanical @entity1931 in ipsilateral hindpaw developed 1 day after surgery and lasted until 60 days and thermal withdrawal latency decreased significantly on the ipsilateral side 10 days after surgery and gradually increased through day 60. The IL-8 receptor inhibitor attenuated the mechanical @entity1931 caused by nucleus pulposus when it was administered on postoperative day 10 and reduced microglial activation and phosphorylated form of mitogen-activated protein kinase (pERK) expression in the spinal dorsal horn. CONCLUSION: IL-8 might be a potential therapeutic target in chronic radicular @entity9 because of disc herniation, CXCL8 inhibitor could be one of its promising therapeutic agents.

Changes in expression of mRNA for interleukin-8 and effects of interleukin-8 receptor inhibitor in the spinal dorsal horn in a XXXX model of @entity890 .

@entity1931 :: ('MESH:D006930', 'Disease') :: ['allodynia', 'hyperalgesia'] @entity2531 :: ('MESH:D013001', 'Disease') :: ['painful degenerative disc disorders'] @entity158 :: ('MESH:D010146', 'Disease') :: ['inflammatory pain', 'Pain', 'pain-related behaviors', 'radicular pain'] @entity35 :: ('10116', 'Species') :: ['rat', 'Rats'] @entity9 :: ('MESH:D009437', 'Disease') :: ['neuropathic pain'] @entity890 :: ('MESH:D007405', 'Disease') :: ['lumbar disc herniation']
@entity35 :: (10116,Species) :: ['rat']
