The aim of this study was to compare the in vitro @entity137 of two hepatotoxins in hepatocyte cultures and in liver slices from both @entity35 and @entity235 . Hepatocytes and liver slices were pre-incubated for 2 hours and then exposed to @entity11485 or @entity1443 , both of which mainly induce @entity409 in vivo. Following exposure to the compounds for 20 hours, neutral red uptake (NRU [hepatocyte cultures only]), @entity98 reduction, and @entity2041 ( @entity2197 ), @entity855 ( @entity855 ) and protein content, were used to measure the @entity137 induced. In general, @entity11485 and @entity1443 exposure caused comparable levels of @entity137 in hepatocyte cultures and in liver slices. For @entity11485 , no consistent differences were seen between hepatocyte cultures and liver slices. With @entity1443 , the toxic effects were generally slightly more pronounced in hepatocyte cultures than in liver slices, and the preparations from @entity235 liver were more sensitive than those from @entity35 liver to @entity1443 exposure. These results are in agreement with previously described species differences in vitro. NRU and @entity2197 content were more sensitive and more consistent endpoints than @entity98 reduction, @entity855 content or protein content. Liver slices appeared to lose viability over the 20 hours in culture. Therefore, it can be concluded that liver slices should only be used in relatively short-term investigations.

@entity409 in in vitro XXXX testing.

@entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity', 'toxicities'] @entity11485 :: ('MESH:D005688', 'Chemical') :: ['galactosamine'] @entity235 :: ('9615', 'Species') :: ['dog', 'dogs'] @entity409 :: ('MESH:D008107', 'Disease') :: ['liver necrosis'] @entity2041 :: ('MESH:D005978', 'Chemical') :: ['reduced glutathione'] @entity2197 :: ('CHEBI:16856', 'Chemical') :: ['GSH'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity1443 :: ('MESH:D000082', 'Chemical') :: ['paracetamol'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['adenosine triphosphate', 'ATP']
@entity137 :: (MESH:D064420,Disease) :: ['toxicity']
