It has been proposed that @entity519 favors the growth of @entity5 cells over normal cells, particularly @entity5 cells carrying @entity876 mutations. Cytogenetic studies of @entity0 have shown that highly complex karyotypes seen in direct harvest preparations are rarely detected after short-term culture. In this study, 34 paired samples of @entity0 and grossly nontumorous tissue from the same breast were cultured at 20 and 5% (12 samples) or 20 and 0% @entity26 (22 samples). Both @entity189 samples and nontumorous tissue survived at 0% @entity26 . Recovery for 24 hours at 20% produced good yields for cytogenetic analysis. Lower @entity26 levels did not specifically stimulate growth of @entity5 cells. Samples with @entity876 mutations showed a consistently increased growth under @entity519 . Culture at 5% @entity26 did not generally reveal more karyotypic abnormalities than found at 20%. In the samples cultured at 0 and 20%, karyotypic abnormalities were detected only in anaerobic @entity519 culture in two cases. Of the only four samples where more complex karyotypes were detected in the low- @entity26 culture, two were @entity876 mutated. @entity519 treatment followed by recovery at 20% @entity26 may thus increase the yield of complex karyotypes from a subset of @entity0 , particularly those with mutated @entity876 .

Effect of XXXX and @entity876 mutation status and cytogenetics of normal and malignant mammary epithelium.

@entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer', 'breast carcinomas'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinoma'] @entity876 :: ('7157', 'Gene') :: ['TP53'] @entity519 :: ('MESH:D000860', 'Disease') :: ['Hypoxic', 'anaerobic hypoxic conditions', 'hypoxic', 'hypoxia']
@entity519 :: (MESH:D000860,Disease) :: ['hypoxia']
