OBJECTIVE: To study the core promoter mutation in chronic @entity1956 and its effect on viral serology. METHODS: @entity97 core promoter gene fragments were amplified by using mismatched PCR combined with a restriction fragment length polymorphism assay. The PCR products were digested with Bcl I and subjected to electrophoresis on agarose gels. RESULTS: We investigated the core promoter mutation in 89 @entity1 with @entity97 DNA positive. The patterns of restriction fragment length polymorphism (RFIP) of the core promoter gene were distinguished and verified by direct sequencing. The combined mutations of @entity2036 (nt) 1762 and 1764 in the core promoter from A to T and G to A were detected in 47 individuals, 21 of 43 HBeAg positive @entity1 and 26 of 46 anti- HBe positive cases were found infected with this combined mutant. These two groups showed no significant difference (P > 0.05). The combined mutation was found in 11 of the 15 @entity1 with medium and severe chronic @entity99 . In 5 chronic @entity1956 @entity1 with coexistence of wild-type and mutant a tendency of superior accumulation of mutant was found. CONCLUSIONS: This study suggests that the core promoter mutations commonly exist in @entity6194 @entity1 . The mutation can only reduce the pre-core mRNA transcribing efficiency, but cannot discontinue the synthesis of HBeAg. The effect on serology in this mutation is different from that in pre-core stop 28 mutation. The superior accumulation of mutations seems relating to the degree of @entity409 .

[Detection of core promoter mutations in chronic XXXX ].

@entity1 :: ('9606', 'Species') :: ['patients'] @entity97 :: ('10407', 'Species') :: ['HBV'] @entity1956 :: ('MESH:D006509', 'Disease') :: ['hepatitis B'] @entity2036 :: ('MESH:D009711', 'Chemical') :: ['nucleotides'] @entity409 :: ('MESH:D008107', 'Disease') :: ['chronic liver disease'] @entity6194 :: ('MESH:D019694', 'Disease') :: ['Chinese chronic hepatitis B'] @entity99 :: ('MESH:D056486', 'Disease') :: ['hepatitis']
@entity1956 :: (MESH:D006509,Disease) :: ['hepatitis B']
