@entity1 basophils have recently been shown to rapidly produce and release @entity3646 and @entity4047 as well as @entity112 and @entity1813 . Since both @entity3646 and @entity4047 can initiate and maintain late phase @entity56 reactions we addressed whether some widely used anti- @entity56 drugs can inhibit the anti-IgE induced release of these cytokines from enriched @entity1 basophils. Basophils were enriched (47-92% purity) by Ficoll density centrifugation followed by elutriation and negative selection of contaminating cells using immunomagnetic beads. Basophils were stimulated with sub-optimal dilutions of anti-IgE in the presence or absence of various drugs and the release of @entity112 and cytokines were measured after 30 min and 4 h, respectively. The @entity2263 agonist @entity10453 , the H1-receptor antagonist @entity9691 and the phosphodiesterase inhibitor @entity2544 inhibited the release of @entity3646 and @entity4047 by more than 50% following 4 h of basophil stimulation with anti-IgE. These drugs also inhibited the release of @entity112 following 30 min stimulation, although with less efficacy than for @entity3646 and @entity4047 . Short preincubation of basophils with @entity10453 or @entity9691 before stimulation gave rise to significantly greater inhibition of @entity112 release but had less effect on the inhibition of cytokine release. The effects of @entity2544 , however, were not significantly affected by preincubation of the cells with the drug. In contrast to the aforementioned drugs, @entity3045 and @entity14746 were ineffective at inhibiting both @entity112 and cytokine release from basophils. These results suggest that a number of anti- @entity56 drugs may mediate their effects, in part, in reducing late phase @entity56 responses due to their actions on @entity3646 and @entity4047 secretion from basophils.

Inhibition of @entity3646 and @entity4047 release from immunologically activated XXXX basophils due to the actions of anti- @entity56 drugs.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity2544 :: ('MESH:D013806', 'Chemical') :: ['theophylline'] @entity4047 :: ('3596', 'Gene') :: ['IL-13'] @entity10453 :: ('MESH:C057823', 'Chemical') :: ['salmeterol'] @entity3045 :: ('MESH:D000420', 'Chemical') :: ['salbutamol'] @entity56 :: ('MESH:D004342', 'Disease') :: ['allergic'] @entity9691 :: ('MESH:D016593', 'Chemical') :: ['terfenadine'] @entity14746 :: ('MESH:D017332', 'Chemical') :: ['cetirizine'] @entity112 :: ('MESH:D006632', 'Chemical') :: ['histamine'] @entity2263 :: ('10242', 'Gene') :: ['beta-2'] @entity3646 :: ('3565', 'Gene') :: ['IL-4', 'interleukin (IL-)4'] @entity1813 :: ('MESH:D015777', 'Chemical') :: ['eicosanoids']
@entity1 :: (9606,Species) :: ['human']
