The existence of retrograde cell @entity204 in sensory dorsal root ganglion (DRG) cells after @entity1897 is well established. However, with respect to retrograde @entity204 after @entity1897 , available data are conflicting. This may partly be due to the cell counting techniques used. In the present study, quantitative morphometric methods have been used to analyse retrograde @entity204 induced by @entity3510 in adult @entity35 . For comparison, DRG cells were also included in the study. The C7 spinal nerve was transected about 10 mm distal to the DRG and exposed to the fluorescent tracer fast blue in order to retrogradely label the spinal motoneurons and DRG cells of the C7 segment. At 1-16 weeks postoperatively, the nuclei of fast-blue-labelled C7 motoneurons and DRG cells were counted in consecutive 50-microm-thick serial sections. For comparison, the physical disector technique and measurements of neuronal density were also used to calculate motoneuron number. The counts of fast-blue-labelled motoneurons revealed a delayed @entity393 amounting to 21% and 31% after 8 and 16 weeks, respectively (P<0.001). The remaining motoneurons exhibited 20% (P<0.05) soma @entity194 . Using the physical disector technique, the @entity393 was 23% (P<0.001) after 16 weeks. Calculations of neuronal density in Nissl-stained sections failed to reveal any @entity393 , although after correction for shrinkage of the ventral horn a 14% (P<0.001) @entity393 was found. The fast-blue-labelled DRG neurons displayed 51% (P<0.001) cell loss after 16 weeks, and the remaining cells showed 22% (P<0.001) soma @entity194 . In summary, cervical @entity3510 induces @entity10747 of both motoneurons and DRG cells. However, to demonstrate the @entity393 adequate techniques for cell counts have to be employed.

Delayed loss of spinal motoneurons after XXXX in adult @entity35 : a quantitative morphological study.

@entity3510 :: ('MESH:D013124', 'Disease') :: ['spinal nerve injury'] @entity10747 :: ('MESH:D012183', 'Disease') :: ['retrograde degeneration'] @entity1897 :: ('MESH:D010523', 'Disease') :: ['peripheral nerve injury'] @entity194 :: ('MESH:D001284', 'Disease') :: ['atrophy'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity393 :: ('MESH:D034381', 'Disease') :: ['motoneuron loss'] @entity204 :: ('MESH:D003643', 'Disease') :: ['motoneuron death', 'death']
@entity1897 :: (MESH:D010523,Disease) :: ['peripheral nerve injury']
