UNASSIGNED: Biofilms are often the cause of chronic @entity1 @entity281 and contaminate industrial or medical equipment. The traditional approach has been to use increasing concentrations of antibiotics, but microorganisms rapidly develop multiresistance to them. Therefore, we investigated the use of natural substances as an alternative solution. The quantification of the biofilms based on the colonized areas was measured using a Cellavista automatic microscope equipped with image analysis software. Using the Cellavista device brings new possibilities for qualification and quantification of sessile cells. In our study, this feature was documented by exploring the antifungal/anti-biofilm activity of @entity2379 , @entity19988 , chitosan and @entity27994 against @entity375 biofilm formation. The influence of these substances on the formation and eradication of opportunistic pathogenic @entity375 @entity7409 and Candida krusei biofilms was studied in 96-well @entity852 microtiter plates. While @entity2379 was not very efficient, the use of @entity19988 and chitosan, even in minimum inhibitory concentrations, was found to rapidly decrease the colonized areas in the wells. The @entity27994 did not display any significant antibiofilm properties even at concentration 300 gml(-1). Our results propose that Cellavista is a promising tool for the study of @entity375 biofilm formation and the effects of antimicrobial agents.

Evaluation of @entity19988 , chitosan and XXXX effect on @entity7409 and Candida krusei biofilm using a Cellavista device.

@entity1 :: ('9606', 'Species') :: ['human'] @entity852 :: ('MESH:D011137', 'Chemical') :: ['polystyrene'] @entity19988 :: ('MESH:C006680', 'Chemical') :: ['baicalein'] @entity2379 :: ('MESH:D000666', 'Chemical') :: ['amphotericin B'] @entity7409 :: ('5480', 'Species') :: ['Candida parapsilosis'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infections'] @entity375 :: ('4932', 'Species') :: ['yeast', 'yeasts'] @entity27994 :: ('MESH:C073339', 'Chemical') :: ['usnic acid']
@entity27994 :: (MESH:C073339,Chemical) :: ['usnic acid']
