Nineteen benign [World Health Organization (WHO) grade I; MI], 21 atypical (WHO grade II; MII), and 19 anaplastic (WHO grade III; MIII) @entity1653 were screened for chromosomal imbalances by comparative genomic hybridization (CGH). These data were supplemented by molecular genetic analyses of selected chromosomal regions and genes. With increasing malignancy grade, a marked accumulation of genomic aberrations was observed; i.e., the numbers (mean +/- SEM) of total alterations detected per @entity5 were 2.9 +/- 0.7 for MI, 9.2 +/- 1.2 for MII, and 13.3 +/- 1.9 for MIII. The most frequent alteration detected in MI was loss on 22q (58%). In MII, aberrations most commonly identified were losses on 1p (76%), 22q (71%), 14q (43%), 18q (43%), 10 (38%), and 6q (33%), as well as gains on 20q (48%), 12q (43%), 15q (43%), 1q (33%), 9q (33%), and 17q (33%). In MIII, most of these alterations were found at similar frequencies. However, an increase in losses on 6q (53%), 10 (68%), and 14q (63%) was observed. In addition, 32% of MIII demonstrated loss on 9p. Homozygous deletions in the @entity986 gene at 9p21 were found in 4 of 16 MIII (25%). Highly amplified DNA sequences were mapped to 12q13-q15 by CGH in 1 MII. Southern blot analysis of this @entity5 revealed amplification of @entity8985 and @entity7465 . By CGH, DNA sequences from 17q were found to be amplified in 1 MII and 8 MIII, involving 17q23 in all cases. Despite the high frequency of chromosomal aberrations in the MII and MIII investigated, none of these @entity5 showed mutations in exons 5-8 of the @entity876 gene. On the basis of the most common aberrations identified in the various malignancy grades, a model for the genomic alterations associated with @entity1653 progression is proposed.

Analysis of genomic alterations in benign, atypical, and @entity1653 : toward a genetic model of XXXX progression.

@entity986 :: ('1029', 'Gene') :: ['CDKN2A'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity7465 :: ('4193', 'Gene') :: ['MDM2'] @entity8985 :: ('1019', 'Gene') :: ['CDK4'] @entity876 :: ('7157', 'Gene') :: ['TP53'] @entity1653 :: ('MESH:D008579', 'Disease') :: ['sporadic meningiomas', 'meningioma']
@entity1653 :: (MESH:D008579,Disease) :: ['meningioma']
