PURPOSE: Epithelial-to-mesenchymal transition (EMT) is an important mechanism in @entity5 @entity3 and @entity3794 . Previous studies demonstrated effect of histone H3 and H4 acetylation in @entity5 and @entity3794 , so we hypothesized that histone modification might play a crucial role in gene regulation during EMT. In this study, we investigated the mechanism behind EMT by analyzing comprehensive gene expression and the effect of @entity1092 (VPA), a class I histone deacetylase inhibitory drug, on histone modification. METHODS: EMT was induced in @entity1 alveolar epithelial cells (A549) using 5  ng/mL of transforming growth factor (TGF)-b1. Various concentrations of VPA were then administered, and Western blotting was used to analyze histone acetylation or methylation. Comprehensive gene expression analysis was carried out by RNA sequencing, and chromatin immunoprecipitation was performed with an anti- @entity16374 histone H3 @entity376 27 antibody. RESULTS: @entity2533 stimulation led to a decrease in histone acetylation, especially that of histone H3K27, and H3K27ac localization was decreased at particular gene loci. This decrease was recovered by VPA treatment, which also up-regulated the mRNA expression of genes down-regulated by @entity2533 , and correlated with the localization of H3K27ac. However, genes up-regulated by @entity2533 stimulation were not suppressed by VPA, with the exception of @entity13239 . CONCLUSIONS: Histone acetylation was down-regulated by @entity2533 stimulation in A549 cells. VPA partially inhibited EMT and the decrease of histone acetylation, which plays an important role in the progression of EMT.

Regulation of Gene Expression by XXXX in Epithelial-to-Mesenchymal Transition.

@entity1 :: ('9606', 'Species') :: ['human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity1092 :: ('MESH:D014635', 'Chemical') :: ['sodium valproate'] @entity16374 :: ('CHEBI:40574', 'Chemical') :: ['acetyl'] @entity3794 :: ('MESH:D011658', 'Disease') :: ['pulmonary fibrosis'] @entity13239 :: ('1277', 'Gene') :: ['COL1A1'] @entity2533 :: ('7040', 'Gene') :: ['TGF-b1'] @entity376 :: ('CHEBI:25094', 'Chemical') :: ['lysine'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis']
@entity1092 :: (MESH:D014635,Chemical) :: ['Sodium Valproate']
