UNASSIGNED: The venom proteome (venomics) of the Sri Lankan @entity9115 was elucidated using 1D SDS PAGE nano-ESI-LCMS/ @entity15 shotgun proteomics. A total of 41 different venom proteins belonging to 11 different protein families were identified. The four main protein families are phospholipase A2 (PLA2, 35.0%), snaclec (SCL, 22.4%, mainly platelet aggregation inhibitors), snake venom @entity563 proteinase (SVSP, 16.0%, mainly Factor V activating enzyme) and snake venom metalloproteinase (SVMP, 6.9%, mainly heavy chain of Factor X activating enzyme). Other protein families that account for more than 1% of the venom protein include @entity14890 oxidase (LAAO, 5.2%), Kunitz-type @entity563 proteinase inhibitor (KSPI, 4.6%), venom nerve growth factor (VNGF. 3.5%), 5'-nucleotidase (5'NUC, 3.0%), @entity462 -rich secretory protein (CRISP, 2.0%) and phosphodiesterase (PDE, 1.3%). The venom proteome is consistent with the enzymatic and toxic activities of the venom, and it correlates with the clinical manifestations of Sri Lankan @entity37335 @entity5900 which include @entity548 , @entity1003 , @entity177 , neuro-myotoxicity and @entity2142 . The venom exhibited remarkable presypnatic @entity2359 presumably due to the action of basic PLA2 in high abundance (35.0%). Besides, SCLs, Factor X activating enzymes (SVMPs), SVSPs, and LAAOs are potential hemotoxins (50.5%), contributing to @entity1003 and @entity548 in Sri Lankan @entity37335 @entity5900 . SIGNIFICANCE: The study demonstrated the proteomic profile of the Sri Lankan @entity9115 venom, unraveling its complex composition of toxins and correlations with major toxic activities. The types, numbers, and relative abundances of toxins were reported. The venom content was dominated by the neurotoxic basic phospholipases A2 (>30% of total protein abundance) and several hemotoxic or coagulopathic protein families (approximately 50% in total). The proteome correlates with the functional and toxinological characterizations of the venom, and reflects the pathophysiological effects of @entity5900 by the Sri Lankan @entity9115 . The venom proteomics may serve to propel the understanding on pathogenesis and treatment strategy for @entity5900 by this viper in Sri Lanka. The enriched database contributed by the proteomic findings will be useful for comparing venom variations among @entity9115 from different geographical areas.

Functional venomics of the Sri Lankan @entity9115 ( XXXX ) and its toxinological correlations.

@entity462 :: ('MESH:D003545', 'Chemical') :: ['cysteine'] @entity9115 :: ('8707', 'Species') :: ['Daboia russelii', "Russell's vipers", "Russell's viper"] @entity548 :: ('MESH:D006470', 'Disease') :: ['hemorrhagic syndrome', 'hemorrhage'] @entity1003 :: ('MESH:D001778', 'Disease') :: ['coagulopathy'] @entity2359 :: ('MESH:D020258', 'Disease') :: ['neurotoxicity'] @entity177 :: ('MESH:D051437', 'Disease') :: ['renal failure'] @entity14890 :: ('CHEBI:15705', 'Chemical') :: ['l-amino acid'] @entity563 :: ('CHEBI:17822', 'Chemical') :: ['serine'] @entity2142 :: ('MESH:D006461', 'Disease') :: ['intravascular hemolysis'] @entity15 :: ('MESH:D009103', 'Disease') :: ['MS'] @entity37335 :: ('435234', 'Species') :: ['D. russelii'] @entity5900 :: ('MESH:D012909', 'Disease') :: ['envenomation']
@entity9115 :: (8707,Species) :: ['Daboia russelii']
