UNASSIGNED: Native konjac glucomannan was used as the substrate for thermophilic actinomycetes, @entity17836 BCRC19214, to produce b-mannanase. The b-mannanase was purified and five internal @entity3689 sequences were determined by LC-MS/MS. These sequences had high homology with the b-mannanase from @entity52433 . The tfm gene which encoded the b-mannanase was cloned, sequenced and heterologous expressed in @entity10057 P01g expression system. Recombinant heterologous expression resulted in extracellular b-mannanase production at levels as high as 3.16U/ml in the culture broth within 48h cultivation. The recombinant b-mannanase from @entity10057 transformant had superior thermal property. The optimal temperature of the recombinant b-mannanase from @entity10057 transformant (pYLSC1-tfm) was 80  C. When native konjac glucomannan was incubated with the recombinant b-mannanase from @entity10057 transformant (pYLSC1-tfm) at 50  C, there was a fast decrease of viscosity happen during the initial phase of reaction. This viscosity reduction was accompanied by an increase of reducing @entity3363 . The surface of konjac glucomannan film became smooth. After 24h of treatment, the DPw of native konjac glucomannan decreased from 6,435,139 to 3089.

Degradation of konjac glucomannan by XXXX thermostable b-mannanase from yeast transformant.

@entity17836 :: ('2021', 'Species') :: ['Thermobifida fusca'] @entity52433 :: ('269800', 'Species') :: ['T. fusca YX'] @entity3689 :: ('D000596', 'Chemical') :: ['amino acid'] @entity10057 :: ('4952', 'Species') :: ['Yarrowia lipolytica', 'Y. lipolytica'] @entity3363 :: ('MESH:D002241', 'Chemical') :: ['sugars']
@entity17836 :: (2021,Species) :: ['Thermobifida fusca']
