UNASSIGNED: In this study, we investigated the suitability of ultrathin and porous @entity32831 (PI) membrane as a carrier for subretinal transplantation of @entity1 embryonic stem cell (hESC) -derived @entity5995 pigment epithelial (RPE) cells in @entity95 . The in vivo effects of hESC-RPE cells were analyzed by subretinal suspension injection into Royal College of Surgeons (RCS) @entity35 . @entity35 eyes were analyzed with electroretinography (ERG) and histology. After analyzing the surface and permeability properties of PI, subretinal PI membrane transplantations with and without hESC-RPE were performed in @entity95 . The @entity95 were followed for three months and eyes analyzed with fundus photography, ERG, optical coherence tomography (OCT), and histology. Animals were immunosuppressed with cyclosporine the entire follow-up time. In @entity3986 @entity35 , ERG and outer nuclear layer (ONL) thickness showed some rescue after hESC-RPE injection. Cells positive for human antigen were found in clusters under the retina 41 days post-injection but not anymore after 105 days. In @entity95 , OCT showed good placement of the PI. However, there was loss of pigmentation on the hESC-RPE-PI over time. In the eyes with PI alone, no obvious signs of @entity32 or retinal @entity194 were observed. In the presence of hESC-RPE, mononuclear cell infiltration and retinal @entity194 were observed around the membranes. The porous ultrathin PI membrane was well-tolerated in the subretinal space and is a promising scaffold for RPE transplantation. However, the rejection of the transplanted cells seems to be a major problem and the given immunosuppression was insufficient for reduction of xenograft induced @entity32 .

Ultrathin XXXX Membrane as Cell Carrier for Subretinal Transplantation of @entity1 Embryonic Stem Cell Derived Retinal Pigment Epithelium.

@entity1 :: ('9606', 'Species') :: ['human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity3986 :: ('MESH:D009136', 'Disease') :: ['dystrophic RCS'] @entity95 :: ('9986', 'Species') :: ['rabbits'] @entity194 :: ('MESH:D001284', 'Disease') :: ['atrophy'] @entity5995 :: ('MESH:D012172', 'Chemical') :: ['retinal'] @entity35 :: ('10116', 'Species') :: ['Rat', 'rats'] @entity32831 :: ('CHEBI:61484', 'Chemical') :: ['polyimide']
@entity32831 :: (CHEBI:61484,Chemical) :: ['Polyimide']
