OBJECTIVE: To investigate the effect of small interfering RNA (siRNA)-mediated inhibition of @entity5 necrosis factor receptor associated factor 6( @entity25433 ) gene on @entity19 odontoblast-like cell line, MDPC-23 cell and the effect of @entity25433 on MDPC-23 cell proliferation. METHODS: The vectors expressing siRNA against @entity25433 were constructed and introduced into MDPC-23 cell with @entity29547 , and the cell line with stable expression of siRNA of @entity25433 was obtained by G418 screening and colony culture. Reverse transcription-PCR (RT-PCR) and Western blotting were performed to detect the expression of @entity25433 . The proliferation of transfected MDPC-23 cell was investigated through @entity37256 ( @entity98 ) and flow cytometry (FCM) assay. RESULTS: The positive single colony was screened out, and was found to express siRNA against @entity25433 effectively because both @entity25433 mRNA and protein relative expression were significantly decreased in the experimental group (pSUPER- @entity25433 siRNA: mRNA 0.163    0.008, protein 0.215    0.006) compared with controls (pSUPER: mRNA 0.778    0.017, protein 0.964    0.007 (P < 0.001). The A value of treated pSUPER- @entity25433 siRNA cells (3 d: 0.46    0.03, 5 d: 1.35    0.06) was increased compared with controls (P<0.01). The result of proliferation index (PrI) was also increased compared with controls [pSUPER- @entity25433 siRNA: (24.1    2.2)%; pSUPER (11.2    1.0)%; control (10.5    0.7)%, P < 0.01]. CONCLUSIONS: The transcription and expression of @entity25433 gene were inhibited. The proliferation ability was increased in MDPC-23 cells by the constructed pSUPER- @entity25433 siRNA vector. It may further influence the formation and repair of dentin, and may be involved in the regulation of normal tooth eruption and process of dentin repair after injury.

[Effect of XXXX necrosis factor receptor associated factor 6 gene silencing by small interfering RNA on proliferation of odontoblast cells].

@entity29547 :: ('MESH:C020888', 'Chemical') :: ['lipofectin'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity37256 :: ('MESH:C011912', 'Chemical') :: ['methabenzthiazuron'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity25433 :: ('22034(Tax:10090)', 'Gene') :: ['TRAF6']
@entity5 :: (MESH:D009369,Disease) :: ['tumor']
