@entity19 were immunized with 1 to 3 micrograms of cytoplasmic @entity172 receptor fragment purified from @entity1 myometrium by affinity chromatography. Two RE-antibody-secreting clones were detected from one fusion that were capable of precipitating cytosol RE. Monoclonal antibody D5 (subclass IgG1) reacts with an antigen that is related to RE from immunoprecipitation studies but which can be separated from the hormone binding unit. In the presence of anti-mouse serum, D5 precipitates labeled @entity1 cytoplasmic RE complexes from @entity0 , fibroid, myometrial, and endometrial preparations but does not react with nuclear RE from @entity1 endometrium or cytoplasmic RE from other species tested. Conversely, antibody C3 (Class IgM) precipitates @entity1 cytoplasmic RE and nuclear RE complexes as well as labeled cytoplasmic RE from @entity35 and @entity291 uterus and @entity1573 oviduct. Neither antibody reacts with @entity1420 receptor or @entity342 receptor from @entity1 @entity0 , @entity443 from @entity1 plasma, or @entity35 @entity12074 . With D5, @entity134 labeling of cytoplasmic RE at 25 degrees increased the RE immune complex precipitated. D5 precipitates molybdate-stabilized RE from myometrial cytosol when labeled at 25 degrees but not at 4 degrees. C5 precipitates molybdate-stabilized RE whether cytosol was @entity134 -labeled at 4 degrees or 25 degrees. For D5, optimal precipitation of RE from @entity1 @entity0 was observed when cytosol was @entity134 -labeled at 25 degrees in buffers of pH range 5 to 6. Immunochemical studies indicate that D5 is associated with a Mr 29,000 component in RE-positive cytosols. Electrofocusing and @entity969 density gradient analysis confirmed that D5 antigen is a non-hormone-binding component related to cytosolic RE from @entity0 and myometrium.

Monoclonal antibodies against a component related to soluble XXXX receptor.

@entity1 :: ('9606', 'Species') :: ['human'] @entity172 :: ('MESH:D004967', 'Chemical') :: ['estrogen'] @entity12074 :: ('24177(Tax:10116)', 'Gene') :: ['alpha-fetoprotein'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast tumor'] @entity1420 :: ('MESH:D011374', 'Chemical') :: ['progesterone'] @entity19 :: ('10090', 'Species') :: ['Mice'] @entity1573 :: ('9031', 'Species') :: ['chick'] @entity134 :: ('CHEBI:35341', 'Chemical') :: ['steroid'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity342 :: ('CHEBI:50113', 'Chemical') :: ['androgen'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity291 :: ('9913', 'Species') :: ['calf'] @entity443 :: ('6462', 'Gene') :: ['SHBG']
@entity172 :: (MESH:D004967,Chemical) :: ['estrogen']
