Between January and September 2003, 39 isolates of the family Enterobacteriaceae with phenotypically positive Vitek 1 extended-spectrum beta-lactamase (ESBL) test results were collected, originating from @entity1 of two hospitals in Saxony, Germany. Plasmid DNA was isolated and screened by PCR for the presence of genes encoding beta-lactamases of SHV, TEM and CTX-M types. To differentiate ESBL and non-ESBL among SHV and TEM genes, detailed analysis of PCR products was performed. Twenty-four strains carried SHV-2, SHV-5 or SHV-12 genes. In a further 11 strains a CTX-M gene was detected. The CTX-M genes could be affiliated to the CTX-M-1 and CTX-M-9 cluster by RFLP analysis. In the case of four @entity7101 isolates, hyperproduction of the chromosomal beta-lactamase K1 was inferred, because genes of the above-mentioned types were not detected. The strains contained plasmid DNA between 45 and 160 kb in size. Common plasmid restriction patterns among SHV-5 producers provided evidence of horizontal spread. Twenty strains had a MIC for @entity3524 of <or=4 mg l-1, 18 strains had the same MIC for @entity5505 , and nine strains had this MIC of >4 mg l-1 for both antibiotics. The ESBL phenotypes often coincided with @entity2177 or @entity801 resistance.

Molecular characterization of extended-spectrum beta-lactamases in Enterobacteriaceae from XXXX of two hospitals in Saxony, Germany.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity801 :: ('MESH:D005839', 'Chemical') :: ['gentamicin'] @entity2177 :: ('MESH:D002939', 'Chemical') :: ['ciprofloxacin'] @entity3524 :: ('MESH:D002439', 'Chemical') :: ['cefotaxime'] @entity7101 :: ('571', 'Species') :: ['Klebsiella oxytoca'] @entity5505 :: ('MESH:D002442', 'Chemical') :: ['ceftazidime']
@entity1 :: (9606,Species) :: ['patients']
