@entity528 of the submucous plexus (IND B) is an indicator of a @entity851 of vegetative gut innervation. It is the mildest form of an @entity2246 . The diagnosis of IND B does not result in a functional conclusion or clinical recommendation but is often accompanied by @entity20254 or an @entity1963 of the rectum. The aim of this study was to demonstrate by morphometric means a way in which the diagnosis of IND B could be made much more reliable. In 20 control subjects, 40 IND B cases and 10 hypoganglionoses with IND B, it was shown that a specific nerve cell staining (e.g. Lactic dehydrogenase, Succinic dehydrogenase, @entity45299 reaction or an immunohistochemical nerve cell staining) was necessary for diagnosis. Cross sections of @entity4494 and cross sections with large nerve cell numbers (> 7 nerve cell profiles) were the most reliable diagnostic criteria. The morphometric examinations were performed with an optic electronic image analysis system. Biopsy serial sections of the rectum-mucosa that contained submucosa demonstrated that 30-40% of the sections contained no @entity4494 . Sixty to 70% of the sections showed ganglia of the submucous plexus. In 100 biopsy sections in subjects with IND B, 20 +/- 5% contained @entity4494 cross sections. In the @entity1 with hypoganglionosis of the submucous plexus, 55 +/- 4% sections had no ganglion and 18 +/- 3% had giant ganglion cross sections. The data demonstrate that for a reliable diagnosis of IND B, at least 30 sections are necessary, stained with a dehydrogenase reaction that contain a minimum of 4 giant ganglion cross sections. These data demonstrate that IND B is not a qualitative diagnosis as @entity1963 but rather a quantitative diagnosis.

Morphometric determination of the methodological criteria for the diagnosis of XXXX (IND B).

@entity1 :: ('9606', 'Species') :: ['patients'] @entity851 :: ('MESH:D006130', 'Disease') :: ['developmental abnormality'] @entity1963 :: ('MESH:D006627', 'Disease') :: ['aganglionosis', "Hirschsprung's disease"] @entity4494 :: ('MESH:D045888', 'Disease') :: ['submucous ganglion', 'giant ganglions'] @entity20254 :: ('MESH:C537394', 'Disease') :: ['oligoneuronal hypoganglionosis of the myenteric plexus'] @entity45299 :: ('1738', 'Gene') :: ['Diaphorase'] @entity528 :: ('MESH:D009410', 'Disease') :: ['Intestinal neuronal dysplasia'] @entity2246 :: ('MESH:D008661', 'Disease') :: ['inborn error of intestinal innervation']
@entity528 :: (MESH:D009410,Disease) :: ['intestinal neuronal dysplasia']
