UNASSIGNED: Feeder cells are generally required for establishment and maintenance of embryonic stem (ES)/induced pluripotent stem (iPS) cells. Increased demands for generation of those cells carrying various types of vectors (i.e., KO vectors and transgenes) also require feeder cells that confer resistance to any types of preexisting selective drugs. Unfortunately, the use of the feeders that are resistant to various drugs appears to be limited to a few laboratories. Here we generated a set of gene-engineered STO feeder cells that confer resistance to several commercially available drugs. The STO cells, which have long been used as a feeder for @entity19 ES and @entity189 ( @entity189 ) cells, were transfected with pcBIH [carrying @entity1732 resistance gene (ble) and @entity14286 phosphotransferase gene (Hyg)], pcBIP [carrying ble and @entity5808 resistance gene (puro)], or pcBSN [carrying ble and @entity5154 resistance gene (neo)]. The resulting stably transfectants (termed SHB for pcBIH, SPB for pcBIP, and SNB for pcBSN) exhibited @entity1732 / @entity13995 , @entity1732 / @entity5808 , or @entity1732 / @entity5154 , as expected. The morphology of these cells passaged over 18 generations was indistinguishable from that of parental STO cells. Of isolated clones, the SHB3, @entity9685 , and SNB2 clones successfully supported the growth of @entity19 ES cells in an undifferentiated state, when coculture was performed. PCR analysis revealed the presence of the selective markers in these clones, as expected. These SHB3, @entity9685 , and SNB2 cells will thus be useful for the acquisition and maintenance of genetically manipulated ES/iPS cells.

Generation of XXXX STO Feeder Cell Lines That Confer Resistance to Several Types of Selective Drugs.

@entity5808 :: ('MESH:D011691', 'Chemical') :: ['puromycin'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity14286 :: ('MESH:D006921', 'Chemical') :: ['hygromycin B'] @entity189 :: ('MESH:D002277', 'Disease') :: ['embryonal carcinoma', 'EC'] @entity9685 :: ('56534(Tax:10090)', 'Gene') :: ['SPB3'] @entity5154 :: ('MESH:D009355', 'Chemical') :: ['neomycin'] @entity1732 :: ('MESH:D001761', 'Chemical') :: ['bleomycin'] @entity13995 :: ('MESH:C026273', 'Chemical') :: ['hygromycin']
@entity19 :: (10090,Species) :: ['Mouse']
