@entity9367 as a main active ingredient of Tripterygium wilfordii is known to be exerting anti-inflammatory, marked immunosuppressive, and podocyte-protective effects. In this study, we investigated the protective effect of @entity9367 in @entity8 . @entity35 glomerular mesangial cells were randomly divided into three groups: (1) control group, (2) @entity1810 (10    g/mL) group, and (3) @entity9367 group ( @entity9367 10    g/L + @entity1810 10    g/L). Sixty male @entity35 were randomly divided into three groups: (1) control group, (2) chronic serum sickness @entity631 model group, and (3) @entity9367 (0.2   mg/kg  d) group. Reverse transcription PCR was used to assess @entity37295 and @entity15927 mRNA expression in the mesangial cells and renal tissues. Western blotting was used to determine @entity37295 and @entity15927 protein expressions. Laser confocal fluorescence microscopy was used to observe the subcellular localization of @entity15927 and @entity37295 proteins in the mesangial cells. @entity9367 inhibited the @entity1810 -induced proliferation of mesangial cells. It significantly upregulated @entity37295 protein expression and downregulated @entity15927 mRNA and protein expressions in a time-dependent manner. Laser confocal fluorescence microscopy detected high @entity15927 fluorescence intensity in the cytoplasm and low @entity15927 and high @entity37295 fluorescence intensity in the nucleus. By upregulating @entity37295 protein expression @entity9367 decreased the extent of @entity82 by affecting the @entity1810 / @entity15927 signaling pathway.

Protective Effect of XXXX against Glomerular Mesangial Cell Proliferation and Glomerular @entity82 in @entity35 Involves the @entity1810 /Smad Signaling Pathway.

@entity8 :: ('MESH:D007674', 'Disease') :: ['kidney disease'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis'] @entity37295 :: ('313757(Tax:10116)', 'Gene') :: ['Ski'] @entity15927 :: ('25631(Tax:10116)', 'Gene') :: ['Smad3'] @entity9367 :: ('MESH:C001899', 'Chemical') :: ['triptolide', 'Triptolide'] @entity1810 :: ('59086(Tax:10116)', 'Gene') :: ['TGF-b1'] @entity35 :: ('10116', 'Species') :: ['Rat', 'Sprague-Dawley rats'] @entity631 :: ('MESH:D005921', 'Disease') :: ['glomerulonephritis']
@entity9367 :: (MESH:C001899,Chemical) :: ['Triptolide']
