AIM: To compare the @entity137 of @entity11379 resin-based root canal sealers with sealers based on @entity990 resin and @entity1346 by two-well established cell culture methods. METHODOLOGY: Specimens of @entity3798 Plus, EndoREZ, RoekoSeal and Epiphany were prepared for direct contact in the Millipore filter diffusion test and as extracts in the @entity98 assay. @entity19 fibroblasts (L929) were used as @entity137 targets. Differences in cytotoxicity between fresh and set specimens and between the extracts of root canal sealers were determined by t-test (P < 0.05). RESULTS: In the filter diffusion test, freshly mixed Epiphany and @entity3798 Plus were rated severely toxic and RoekoSeal and EndoREZ nontoxic. When set, Epiphany was moderately toxic, whereas @entity3798 Plus, RoekoSeal and EndoREZ were nontoxic. Epiphany was significantly more toxic than RoekoSeal and EndoREZ (P < 0.05). In the @entity98 assay with set specimens, Epiphany was rated severely toxic; @entity3798 Plus and RoekoSeal slightly toxic; and EndoREZ nontoxic. Epiphany was significantly more toxic than the other three materials in this test (P < 0.001). CONCLUSION: The multi- @entity11379 resin-based (Epiphany) root canal sealer was significantly more toxic to L-929 cells than the @entity1346 -based Roeko Seal and the single @entity11379 -based EndoREZ root canal sealers. @entity3798 Plus showed intermediate @entity137 .

XXXX evaluation of root canal sealers in vitro.

@entity3798 :: ('MESH:D007039', 'Disease') :: ['AH'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity11379 :: ('CHEBI:25218', 'Chemical') :: ['methacrylate'] @entity19 :: ('10090', 'Species') :: ['Mouse'] @entity990 :: ('CHEBI:30721', 'Chemical') :: ['epoxy'] @entity1346 :: ('MESH:D012828', 'Chemical') :: ['silicone'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT']
@entity137 :: (MESH:D064420,Disease) :: ['Toxicity']
