UNASSIGNED: @entity460 is a universal signal in the regulation of wide aspects in biology, but few are known about the function of @entity460 in the control of early embryo development. @entity141 deficiency in soil induces early @entity5852 in peanut, producing empty pods, which is a general problem; however, the underlying mechanism remains unclear. In this study, @entity5852 was characterized to be caused by apoptosis marked with cell wall degradation. Using a method of SSH cDNA libraries associated with library lift (SSHaLL), 62 differentially expressed genes were isolated from young peanut embryos. These genes were classified to be stress responses, catabolic process, @entity1735 and lipid metabolism, embryo morphogenesis, regulation, etc. The cell retardation with cell wall degradation was caused by up-regulated cell wall hydrolases and down-regulated cellular synthases genes. HsfA4a, which was characterized to be important to embryo development, was significantly down-regulated under @entity141 -deficient conditions from 15  days after pegging (DAP) to 30 DAP. Two AhCYP707A4 genes, encoding @entity2271 ( @entity2272 ) 8'-hydroxylases, key enzymes for @entity2272 catabolism, were up-regulated by 21-fold under @entity141 -deficient conditions upstream of HsfA4a, reducing the @entity2272 level in early embryos. Over-expression of AhCYP707A4 in Nicotiana benthamiana showed a phenotype of low @entity2272 content with high numbers of @entity5852 , small pods and less seeds, which confirms that AhCYP707A4 is a key player in regulation of @entity141 @entity5852 via @entity2272 -mediated apoptosis. The results elucidated the mechanism of low @entity141 -induced @entity5852 and described the method for other fields of study.

Identification of low XXXX stress-induced embryo apoptosis response genes in Arachis hypogaea by SSH-associated library lift (SSHaLL).

@entity2272 :: ('CHEBI:2365', 'Chemical') :: ['ABA'] @entity2271 :: ('MESH:D000040', 'Chemical') :: ['abscisic acid'] @entity5852 :: ('MESH:D000031', 'Disease') :: ['aborted embryos', 'embryo abortion', 'deficiency-induced embryo abortion'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['carbohydrate'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium', 'Calcium'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)']
@entity141 :: (CHEBI:29108,Chemical) :: ['Ca(2+)']
