UNASSIGNED: Severe @entity130 produces a plethora of @entity209 which contribute to the prolonged morbidity of burn survivors. The authors have recently demonstrated trans-differentiation of @entity1386 ( @entity1386 ) after burn @entity130 , toward a more thermogenic phenotype. However, the impact of @entity130 on subcutaneous @entity1386 (sWAT) morphology in @entity1 is unknown. Here, the authors studied the effect of severe @entity130 on the architecture of sWAT. sWAT was collected from 11 severely burned @entity1 (11    3 years; 55    16% total BSA burned) and 12 nonburned healthy @entity1 (9    3 years). Histology, electron microscopy, immunohistochemistry, and immunofluorescence were performed on fixed adipose tissue sections. sWAT cytokine and collagen concentrations were measured by multiplex assay and sirius/fast green staining method, respectively. sWAT histology demonstrated multiple fat droplets, significantly (P < .05) reduced mean cell size (104    6 vs 68    3  m) and higher collagen content (7    0.8 vs 4    0.4) in burn @entity1 . sWAT from burn victims stained positive for @entity469 suggesting infiltration of macrophages. Furthermore, electron microscopic analysis showed multiple fat droplets and greater mitochondrial abundance in sWAT of burn survivors. In agreement with this, mitochondrial respiratory capacity in the leak and coupled state increased by 100% in sWAT of burned @entity1 from 1 to 3 weeks postinjury. The cytokines @entity650 , @entity687 , @entity4047 , @entity1192 , @entity1356 , @entity2842 , and @entity310 were all significantly greater in the sWAT of burned @entity1 versus healthy @entity1 (P < .05). Furthermore, @entity650 , @entity687 , @entity1192 , @entity1356 , and @entity310 significantly increased after injury in sWAT of burned @entity1 (P < .05). This study provides detailed evidence of morphological and functional changes in sWAT of burn survivors which was associated with tissue @entity32 . A better understanding of morphological and functional changes in sWAT will help discern the mechanisms underlying @entity2495 in burned @entity1 .

Morphological Changes in Subcutaneous White Adipose Tissue After Severe XXXX .

@entity1 :: ('9606', 'Species') :: ['children', 'humans', 'patients'] @entity650 :: ('3569', 'Gene') :: ['IL-6'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity4047 :: ('3596', 'Gene') :: ['IL-13'] @entity1356 :: ('3553', 'Gene') :: ['IL-1b'] @entity310 :: ('7124', 'Gene') :: ['TNF-a'] @entity469 :: ('968', 'Gene') :: ['CD68'] @entity2842 :: ('6347', 'Gene') :: ['MCP-1'] @entity2495 :: ('MESH:C565498', 'Disease') :: ['hypermetabolism'] @entity1192 :: ('3552', 'Gene') :: ['IL-1a', 'IL1-a'] @entity209 :: ('MESH:D008659', 'Disease') :: ['metabolic abnormalities'] @entity687 :: ('3576', 'Gene') :: ['IL-8'] @entity1386 :: ('MESH:D018205', 'Disease') :: ['white adipose tissue', 'WAT'] @entity130 :: ('MESH:D014947', 'Disease') :: ['burn injury', 'trauma']
@entity130 :: (MESH:D014947,Disease) :: ['Burn Injury']
