@entity1 esophageal epithelium is continuously exposed to physical stimuli or to gastric acid that sometimes causes @entity32 of the mucosa. @entity13812 ( @entity13812 ) is a nociceptive, @entity141 -selective ion channel activated by @entity2951 , heat, and protons. It has been reported that activation of @entity13812 expressed in esophageal mucosa is involved in @entity354 ( @entity354 ) or in nonerosive @entity354 symptoms. In this study, we examined the expression and function of @entity13812 in the @entity1 esophageal epithelial cell line Het1A, focusing in particular on the role of oxidative stress. @entity687 ( @entity687 ) secreted by @entity1 cells upon stimulation by @entity2951 or acid with/without @entity11838 ( @entity13789 ) was measured by ELISA. Following @entity2951 stimulation, the intracellular production of reactive @entity26 species (ROS) was determined using a redox-sensitive fluorogenic probe, and ROS- and @entity13789 -modified proteins were determined by Western blotting using biotinylated @entity462 and anti- @entity13789 antibody, respectively. @entity13789 modification of @entity13812 proteins was further investigated by immunoprecipitation after treatment with synthetic @entity13789 . @entity2951 and acid induced @entity687 production in @entity1 cells, and this production was diminished by antagonists of @entity13812 . @entity2951 also significantly increased the production of intracellular ROS and ROS- or @entity13789 -modified proteins in @entity1 cells. Moreover, @entity687 production in @entity2951 -stimulated @entity1 cells was enhanced by synthetic @entity13789 treatment. Immunoprecipitation studies revealed that @entity13812 was modified by @entity13789 in synthetic @entity13789 -stimulated @entity1 cells. We concluded that @entity13812 functions in chemokine production in esophageal epithelial cells, and this function may be regulated by ROS via posttranslational modification of @entity13812 .

Oxidative stress-induced posttranslational modification of XXXX expressed in esophageal epithelial cells.

@entity1 :: ('9606', 'Species') :: ['human', 'Human', 'Het1A'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity462 :: ('MESH:D003545', 'Chemical') :: ['cysteine'] @entity2951 :: ('MESH:D002211', 'Chemical') :: ['Capsaicin', 'capsaicin'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity354 :: ('MESH:D005764', 'Disease') :: ['gastroesophageal reflux disease', 'GERD'] @entity13789 :: ('CHEBI:58968', 'Chemical') :: ['HNE'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity687 :: ('3576', 'Gene') :: ['Interleukin-8', 'IL-8'] @entity11838 :: ('MESH:C027576', 'Chemical') :: ['4-hydroxy-2-nonenal'] @entity13812 :: ('7442', 'Gene') :: ['TRPV1', 'Transient receptor potential vanilloid 1']
@entity13812 :: (7442,Gene) :: ['TRPV1']
