UNASSIGNED: @entity64 is a major cause of @entity204 and disability worldwide. However, treatment options to date are very limited. To meet the need for validating the novel therapeutic approaches and understanding the physiopathology of the @entity63 @entity1545 , experimental @entity64 models were critical for preclinical research. However, commonly used @entity64 models are reluctant to mimic the clinical situation and not suitable for thrombolytic timing studies. In this paper, we established a standard method for producing a @entity35 @entity64 model with autologous @entity715 formed within the common carotid artery (CCA) by constant galvanic stimulation. Then the @entity715 was shattered and channeled into the origin of the MCA and small (lacunar) artery. To identify the success of @entity2500 , regional cerebral blood flow was monitored, @entity51 and @entity1188 volumes were measured at 2, 4 and 6h postischemia. This model developed a predictable @entity1188 volume (38.37  2.88%) and gradually reduced blood flow (20% of preischemic baselines) within the middle cerebral artery (MCA) territory. The @entity715 occluded in the MCA was able to be lysed by a @entity2435 ( @entity2435 ) within 4h postischemia. The techniques presented in this paper would help investigators to overcome technical problems for @entity64 research.

A novel @entity2500 cerebral artery occlusion model induced by @entity715 formed in common carotid artery in XXXX .

@entity1545 :: ('MESH:D020214', 'Disease') :: ['brain injury'] @entity64 :: ('MESH:D020521', 'Disease') :: ['embolic stroke', 'stroke', 'Stroke'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemic'] @entity2500 :: ('MESH:D020244', 'Disease') :: ['MCA occlusion'] @entity51 :: ('MESH:D009461', 'Disease') :: ['neurological deficits'] @entity1188 :: ('MESH:D007238', 'Disease') :: ['infarct'] @entity2435 :: ('25692(Tax:10116)', 'Gene') :: ['t-PA', 'tissue-type plasminogen activator'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity715 :: ('MESH:D013927', 'Disease') :: ['thrombus'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death']
@entity35 :: (10116,Species) :: ['rat']
