BACKGROUND: Circulating endothelial cells (CECs) have been studied as a biomarker for @entity5 progression and monitoring therapeutic effects. The CellSearch system is a semi-automated system that allows standardised analysis of CECs. This study assessed the clinical implications of CECs determined by the CellSearch system in @entity0 @entity1 . METHODS: Seventy-six consecutive @entity0 @entity1 (53 operable and 23 metastatic or recurrent) were enrolled for the study. Thirty-five @entity1 with operable @entity0 received preoperative chemotherapy with a regimen based on @entity4349 and/or @entity3907 . CECs are defined as CD146(+)CD105(+) @entity1195 (-)DAPI(+) cells in the system. @entity2280 expression was examined using the additional channel in the system. RESULTS: A majority (4539 of 5183 cells, 88%) of CECs from @entity1 with operable @entity0 were @entity2280 -positive. @entity5 showed higher baseline CEC and @entity2280 (+)CEC counts than the other types (P=0.0387 and 0.0377, respectively). Low baseline CEC and @entity2280 (+)CEC counts, and a low @entity2280 positive rate were associated with pathological complete response (pCR) of preoperative chemotherapy in @entity1 with primary @entity0 (P=0.046, 0.027 and 0.01, respectively). In multivariate analyses, the @entity2280 positive rate was significant for pCR (P=0.021). During preoperative chemotherapy, CEC and @entity2280 (+)CEC counts before each cycle of chemotherapy increased with @entity3907 -based regimens (P=0.0018 and 0.0008, respectively) but not with @entity4349 -based regimens. CONCLUSIONS: Baseline CEC, in particular @entity2280 (+)CEC, counts and the @entity2280 positive rate might be useful for the prediction of treatment response of preoperative chemotherapy in @entity1 with operable @entity0 .

Determining circulating endothelial cells using CellSearch system during preoperative systemic chemotherapy in @entity0 XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity4349 :: ('CHEBI:48120', 'Chemical') :: ['anthracycline'] @entity5 :: ('MESH:D009369', 'Disease') :: ['Triple-negative cancers', 'tumour'] @entity1195 :: ('5788', 'Gene') :: ['CD45'] @entity3907 :: ('MESH:C080625', 'Chemical') :: ['taxane'] @entity2280 :: ('947', 'Gene') :: ['CD34']
@entity1 :: (9606,Species) :: ['patients']
