@entity4338 cotransporters (KCCs) play a fundamental role in epithelial cell function, both in the context of ionic homeostasis and also in cell morphology, cell division and locomotion. Unlike other ubiquitously expressed KCC isoforms, expression of @entity60000 is widely considered to be restricted to neurons, where it is responsible for maintaining a low intracellular @entity2865 concentration to drive hyperpolarising postsynaptic responses to the inhibitory neurotransmitters @entity1246 and @entity2228 . Here we report a novel finding that @entity60000 is widely expressed in several @entity1 @entity5 cell lines including the @entity5 cell line (SiHa). Membrane biotinylation assays and immunostaining showed that endogenous @entity60000 is located on the cell membrane of @entity1 cells. To elucidate the role of @entity60000 in cervical tumuorigenesis, @entity1 cells with stable overexpression or knockdown of @entity60000 were employed. Overexpression of @entity60000 had no significant effect on cell proliferation but dramatically suppressed cell spreading and stress fibre organization, while knockdown of @entity60000 showed opposite effects. In addition, @entity442 ( @entity442 )-induced cell migration and invasiveness were significantly increased by overexpression of @entity60000 . @entity60000 -induced cell migration and invasion were not dependent on @entity60000 transport function since overexpression of an activity-deficient mutant @entity60000 still increased @entity442 -induced cell migration and invasion. Moreover, overexpression of @entity60000 significantly diminished the number of focal adhesions, while knockdown of @entity60000 increased their number. Taken together, our data establish that @entity60000 expression and function are not restricted to neurons and that @entity60000 serves to increase cervical tumourigenesis via an ion transport-independent mechanism.

The potassium- XXXX cotransporter 2 promotes @entity5 cell migration and invasion by an ion transport-independent mechanism.

@entity1 :: ('9606', 'Species') :: ['human', 'SiHa'] @entity1246 :: ('MESH:D005680', 'Chemical') :: ['GABA'] @entity2228 :: ('CHEBI:57305', 'Chemical') :: ['glycine'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer', 'cervical cancer'] @entity60000 :: ('57468', 'Gene') :: ['KCC2'] @entity442 :: ('3479', 'Gene') :: ['insulin-like growth factor 1', 'IGF-1'] @entity2865 :: ('CHEBI:17996', 'Chemical') :: ['chloride'] @entity4338 :: ('CHEBI:32588', 'Chemical') :: ['K(+)-Cl(-)']
@entity2865 :: (CHEBI:17996,Chemical) :: ['chloride']
