An immortal, cloned cell line (RCMH), obtained from @entity1 skeletal muscle was established in our laboratory and shown to express muscle specific proteins. We measured ligand binding to ion channels, ion currents using whole cell patch clamp and intracellular @entity460 both in cells grown in complete media and in cells grown for 4-40 days in media supplemented with hormones and nutrients (differentiating media). Markers for differentiated muscle, such as the muscle isoform of @entity908 kinase and the cytoskeletal proteins @entity8859 , alpha-sarcomeric actin, @entity3999 and @entity10484 were present in early stages. Receptors for gamma toxin from Tityus serrulatus scorpion venom, a specific modulator for voltage dependent sodium channels, were present (0.9-1.0 pmol mg-1 protein) during stage 1 (0-6 days in culture with differentiating media) and increased by 50% in stage 3 (more than 10 days in differentiating media). High and low affinity @entity7236 receptors present in stage 1 convert into a single type of high affinity receptors in stage 3. Both intracellular @entity460 release and InsP3 receptors were evident in stage 1 but @entity8452 receptors were expressed only in stage 3. RCMH cells showed no voltage sensitive currents in stage 1. Between 7 and 10 days in differentiating media (stage 2), an outward @entity972 current was observed. Small inward currents appeared only in stage 3; we identified both @entity1022 sensitive and tetrodotoxin resistant @entity1372 currents as well as @entity460 currents. This pattern is consistent with the expression of voltage dependent @entity460 release before appearance of both the action potential and @entity8452 receptors.

Expression of ion channels during differentiation of a XXXX skeletal muscle cell line.

@entity1 :: ('9606', 'Species') :: ['human'] @entity7236 :: ('MESH:C038806', 'Chemical') :: ['dihydropyridine'] @entity908 :: ('MESH:D003401', 'Chemical') :: ['creatine'] @entity8452 :: ('MESH:D012433', 'Chemical') :: ['ryanodine'] @entity1022 :: ('MESH:D013779', 'Chemical') :: ['tetrodotoxin'] @entity1372 :: ('MESH:D012964', 'Chemical') :: ['sodium'] @entity10484 :: ('7273', 'Gene') :: ['titin'] @entity3999 :: ('79784', 'Gene') :: ['myosin'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity972 :: ('MESH:D011188', 'Chemical') :: ['potassium'] @entity8859 :: ('87', 'Gene') :: ['alpha-actinin']
@entity1 :: (9606,Species) :: ['human']
