Tissue pieces of @entity95 fetal lung, 23 days gestation, were cultured for 7 days in serum-free medium to obtain lamellar body material for phospholipid analysis. Cultures were maintained in culture medium without serum and (1) with no added hormones (control cultures), (2) with @entity1955 (1 x 10(-7) M), (3) with @entity701 (1 x 10(-7) M) and (4) with @entity1955 plus @entity701 (1 x 10(-7) M each). The hormonal response was evaluated by measuring the quantity of lamellar body material isolated from the tissue pieces after the 7-day culture period. Compared to control cultures, more lamellar body material was recovered from cultures treated with @entity701 (180% of control) and with @entity1955 plus @entity701 (250% of control). Cultures treated with @entity1955 alone yielded the same amount of lamellar body material as the controls. Hormone treatment produced only minor changes in the glycerophospholipid profile of the lamellar body material. A small but significant increase in the percentage of @entity3634 and a small but significant decrease in @entity4357 were found in lamellar body material from cultures treated with @entity1955 and @entity1955 plus @entity701 . The disaturated @entity2434 content of the lamellar body material from culture was 28% of the total lamellar body phospholipid and was not affected by hormone treatment. This disaturated @entity2434 content was low compared to the disaturated @entity2434 of lamellar body material from adult lung (46%). The low proportion of disaturated @entity2434 was due to the unusual presence of @entity20765 (16:1(cis-9)), which was more than one-fourth of the total @entity844 of the lamellar body @entity2434 . It is possible that an abnormal delta 9 @entity844 desaturation activity was expressed in the lung tissue in vitro, which resulted in the high incorporation of the 16:1 @entity844 into lamellar body @entity2434 .

The phospholipids of lamellar body material from fetal @entity95 lung tissue in culture. Unusual XXXX @entity844 profile.

@entity844 :: ('CHEBI:35366', 'Chemical') :: ['fatty acid'] @entity20765 :: ('MESH:C008757', 'Chemical') :: ['palmitoleic acid'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity1955 :: ('MESH:D013974', 'Chemical') :: ['thyroxine'] @entity2434 :: ('MESH:D010713', 'Chemical') :: ['phosphatidylcholine'] @entity701 :: ('MESH:D006854', 'Chemical') :: ['cortisol'] @entity4357 :: ('MESH:D010716', 'Chemical') :: ['phosphatidylinositol'] @entity3634 :: ('MESH:D010715', 'Chemical') :: ['phosphatidylglycerol']
@entity2434 :: (MESH:D010713,Chemical) :: ['phosphatidylcholine']
