We investigated the pathogenicity, invasiveness, and genetic relatedness of 17 clinical @entity5125 stains isolated over a period of nine years (2006-2014). All isolates were phenotypically characterised and growth patterns were determined. The antimicrobial susceptibility of @entity5125 isolates was determined in E-tests. Invasion assays were performed with epithelial HeLa cells. Finally, @entity5125 isolates were subtyped by PFGE and MLST. All isolates had similar phenotypic characteristics (b-haemolysis and lecithinase activity), and three types of growth curve were observed. Bacterial recovery rates after invasion assays ranged from 0.09% to 7.26% (1.62    0.46). MLST identified 11 sequence types (STs), and 14 PFGE profiles were obtained, indicating a high degree of genetic diversity. Genetic studies unequivocally revealed the occurrence of one outbreak of listeriosis in @entity1 that had not previously been reported. This outbreak occurred in October 2009 and affected three @entity1 from neighbouring towns. In conclusion, the molecular epidemiological analysis clearly revealed a cluster (three @entity1 cases, all @entity28620 ) of not previously reported listeriosis cases in northwestern Spain. Our findings indicate that molecular subtyping, in combination with epidemiological case analysis, is essential and should be implemented in routine diagnosis, to improve the tracing of the sources of outbreaks.

Molecular Epidemiology of Invasive Listeriosis due to XXXX in a Spanish Hospital over a Nine-Year Study Period, 2006-2014.

@entity1 :: ('9606', 'Species') :: ['humans', 'human', 'patients'] @entity28620 :: ('6386', 'Gene') :: ['ST1'] @entity5125 :: ('1639', 'Species') :: ['Listeria monocytogenes', 'L. monocytogenes']
@entity5125 :: (1639,Species) :: ['Listeria monocytogenes']
