UNASSIGNED: Extracellular @entity2036 are signaling elements present in the @entity5 microenvironment; however, their role in @entity5 growth is not completely understood. In the present study, we asked whether @entity2036 regulate cell @entity4222 in @entity43 -derived cells. We observed that 100    M @entity856 induced @entity4222 in SKOV-3 cells (1.57        0.08 fold over basal), and RT-PCR showed expression of transcripts for the @entity9897 and @entity29445 receptors. Knockdown of @entity9897 expression in SKOV-3 cells ( @entity9897 -KD) abolished the @entity856 -induced @entity4222 . The mechanism activated by @entity856 to induce @entity4222 involves transactivation of the @entity470 ( @entity470 ) since we observed that the @entity470 kinase inhibitor @entity20830 and the @entity10412 inhibitor @entity2307 inhibit this response (to 0.76        0.23 and 0.46        0.14 relative to the control, respectively). In agreement with these observations, @entity856 was able to modify the phosphorylation state of the @entity470 ; likewise, the induction of @entity1366 phosphorylation promoted by @entity856 was abolished by a 30-60   min treatment with @entity20830 . Our data also suggested that the enhanced cell @entity4222 involves the epithelium to mesenchymal transition (EMT) process, since a 12   h stimulation of SKOV-3 cells with 100    M @entity856 showed an increase in @entity2277 and @entity4245 protein levels (459.8        132.4% over basal for @entity4245 ). Interestingly, treatment with apyrase (10   U/mL) reduces the @entity4222 of control cells and induces a considerable enrichment of @entity5962 in the cell-cell contacts, favoring an epithelial phenotype and strongly suggesting that the @entity2036 released by @entity5 cells and acting through the @entity9897 receptor are potential regulators of invasiveness. J. Cell. Biochem. 117: 1016-1026, 2016.    2015 Wiley Periodicals, Inc.

The XXXX Receptor Induces Carcinoma Cell Migration and EMT Through Cross-Talk With @entity470 .

@entity43 :: ('MESH:D010051', 'Disease') :: ['ovarian carcinoma'] @entity4245 :: ('6615', 'Gene') :: ['SNAIL'] @entity2036 :: ('MESH:D009711', 'Chemical') :: ['nucleotides'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity4222 :: ('MESH:D014085', 'Disease') :: ['migration'] @entity9897 :: ('5029', 'Gene') :: ['P2RY2'] @entity470 :: ('1956', 'Gene') :: ['EGFR', 'epidermal growth factor receptor'] @entity5962 :: ('999', 'Gene') :: ['E-cadherin'] @entity2307 :: ('MESH:C009687', 'Chemical') :: ['Wortmannin'] @entity856 :: ('MESH:D014544', 'Chemical') :: ['UTP'] @entity10412 :: ('5293', 'Gene') :: ['PI3K'] @entity2277 :: ('7431', 'Gene') :: ['vimentin'] @entity20830 :: ('MESH:C101044', 'Chemical') :: ['AG1478'] @entity1366 :: ('5595;5594', 'Gene') :: ['ERK1/2'] @entity29445 :: ('5030', 'Gene') :: ['P2RY4']
@entity9897 :: (5029,Gene) :: ['P2RY2']
