Following @entity130 to the spinal cord, hematogenous inflammatory cells including neutrophils, monocytes, and lymphocytes infiltrate the lesion in a distinct temporal sequence. To examine potential mechanisms for their recruitment, we measured chemokine mRNAs in the contused @entity35 spinal cord, using specific and sensitive reverse transcriptase polymerase chain reaction (RT-PCR) dot-blot hybridization assays. The neutrophil chemoattractant GRO-alpha was 30-fold higher than control values at 6 hr postinjury and decayed rapidly thereafter. LIX, a highly related alpha-chemokine, also was elevated early postinjury. @entity657 and @entity43290 mRNAs, potent chemoattractants for monocytes, were significantly elevated at the lesion epicenter at 12 and 24 hr postinjury and declined thereafter. @entity16308 ( @entity16308 ), chemoattractant towards activated T-lymphocytes, was significantly elevated at 6 and 12 hr postinjury. The dendritic cell chemoattractant @entity21463 also was increased, perhaps contributing to the development of T-cell autoreactivity to neural components after @entity1566 ( @entity1566 ) in @entity35 . Other beta-chemokines, including @entity9269 and @entity16620 (regulated on expression normal T-cell expressed and secreted), were minimally affected by @entity1566 . Expression of chemokines, therefore, directly precedes the influx of target neutrophils, monocytes, and T-cells into the spinal cord postinjury, as noted previously. Thus, selective chemokine expression may be integral to inflammatory processes within the injured spinal cord as a mechanism of recruitment for circulating leukocytes.

Selective chemokine mRNA accumulation in the XXXX spinal cord after contusion injury.

@entity9269 :: ('25542(Tax:10116)', 'Gene') :: ['MIP-1alpha'] @entity16620 :: ('81780(Tax:10116)', 'Gene') :: ['RANTES'] @entity657 :: ('24770(Tax:10116)', 'Gene') :: ['Monocyte chemoattractant peptide (MCP)-1'] @entity43290 :: ('287562(Tax:10116)', 'Gene') :: ['MCP-5'] @entity21463 :: ('29538(Tax:10116)', 'Gene') :: ['MIP-3alpha'] @entity16308 :: ('245920(Tax:10116)', 'Gene') :: ['Interferon-gamma-inducible protein, 10 kDa', 'IP-10'] @entity1566 :: ('MESH:D013119', 'Disease') :: ['spinal cord injury', 'SCI'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity130 :: ('MESH:D014947', 'Disease') :: ['traumatic injury']
@entity35 :: (10116,Species) :: ['rat']
