INTRODUCTION: Acidic dentifrices have been shown to be more effective than neutral ones against @entity1547 using in vitro, in situ, and clinical protocols. OBJECTIVES: Given the scarcity of studies assessing intraoral @entity1388 (F) retention after using such formulations, the present study evaluated the influence of pH and F concentration of dentifrices on F uptake by saliva, biofilm, and biofilm fluid. METHODS: Volunteers (n   =   22) were randomly assigned to dentifrices containing 0 (placebo), 550 (LFD, low- @entity1388 dentifrice), and 1100  ppm  F ( @entity1050 , conventional dentifrice) at pH  4.5 and 7.0 and brushed their teeth 3 times/day following a double-blind, crossover protocol. Saliva and biofilm samples were collected after 7  days of using the dentifrices, 1 and approximately 12  h after last brushing. F and @entity460 ( @entity9495 ) analyses were performed with the inverted electrode after buffering with TISAB III and the Arsenazo III method, respectively. Data were analyzed by 3-way ANOVA, Tukey's HSD test, and Pearson's correlation coefficient (p   <   0.05). RESULTS: F concentrations in biofilm fluid and whole biofilm 1  h after brushing with acidic F-toothpastes were higher than those related to neutral counterparts, although the differences were small and not significant; no increases were observed in salivary F concentrations influenced by dentifrice pH. Moreover, no definite trend was observed for @entity9495 concentrations in these compartments. CONCLUSION: Dentifrice pH had some influence on F uptake by the biofilm fluid, having lesser or no impact on F uptake by the biofilm and saliva, respectively. CLINICAL SIGNIFICANCE: Toothbrushing with acidic toothpastes leads to slight increases in F concentrations in the biofilm fluid when compared to neutral formulations, which may contribute to the higher anticaries effect of acidic formulations.

Effects of pH and XXXX concentration of dentifrices on @entity1388 levels in saliva, biofilm, and biofilm fluid in vivo.

@entity1050 :: ('MESH:D006223', 'Disease') :: ['CD'] @entity1547 :: ('MESH:D003731', 'Disease') :: ['dental caries'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity1388 :: ('MESH:D005459', 'Chemical') :: ['fluoride'] @entity9495 :: ('D002118', 'Chemical') :: ['Ca']
@entity1388 :: (MESH:D005459,Chemical) :: ['fluoride']
