PURPOSE: To investigate the role of interferon (IFN)-y in @entity936 -associated conjunctival apoptosis. METHODS: Desiccating stress (DS) was created in C57BL/6 (B6) and C57BL/6 IFN-y-knockout (B6yKO) @entity19 . A separate group of @entity19 of both strains also received subconjunctival injections of exogenous IFN-y or vehicle control (BSA) at days 0, +2, and +4 after DS. Immunoreactivity to active (Ac)- @entity29578 and terminal deoxynucleotidyl transferase-mediated @entity1977 -digoxigenin nick end labeling (TUNEL) were evaluated in cryosections. Goblet cell apoptosis was assessed by @entity30927 and TUNEL double staining. Levels of @entity185 , Fas, and Fas-associated protein with Death Domain ( @entity51424 ) mRNA in conjunctiva were measured by real-time PCR. The activity of @entity29578 was measured using fluorometric assay. RESULTS: Increased Ac- @entity34645 and TUNEL immunoreactivity were noted in conjunctival epithelia in B6 @entity19 compared with B6yKO @entity19 after DS, and exogenous IFN-y administration further increased these parameters. DS-induced conjunctival apoptosis was greatest in the goblet cell area and was accompanied by a decrease in @entity30927 expression in the B6 and B6-IFN-y-injected groups compared with the B6yKO and B6-BSA-injected groups. B6yKO @entity19 were resistant to DS-induced apoptosis; however, B6yKO receiving IFN-y yielded results similar to those for B6 wild-type. @entity7349 production and activity were not increased with DS in B6 or B6yKO @entity19 ; however, the administration of IFN-y significantly increased @entity7349 production and activity in both strains compared with vehicle-injected @entity19 . CONCLUSIONS: IFN-y plays a pivotal role in exacerbating conjunctival apoptosis through dual apoptotic pathways with DS.

Interferon-y exacerbates XXXX -induced apoptosis in conjunctiva through dual apoptotic pathways.

@entity34645 :: ('12367;12370', 'Gene') :: ['caspase-3 and -8'] @entity30927 :: ('17833(Tax:10090)', 'Gene') :: ['MUC5AC'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity1977 :: ('CHEBI:17625', 'Chemical') :: ['dUTP'] @entity185 :: ('12367(Tax:10090)', 'Gene') :: ['caspase-3, -8, -9'] @entity7349 :: ('12371(Tax:10090)', 'Gene') :: ['caspase-9', 'Caspase-9'] @entity936 :: ('MESH:D015352', 'Disease') :: ['dry eye'] @entity29578 :: ('12367;12370;12371', 'Gene') :: ['caspase-3, -8, or -9', 'caspase-3, -8, and -9'] @entity51424 :: ('14082(Tax:10090)', 'Gene') :: ['FADD']
@entity936 :: (MESH:D015352,Disease) :: ['dry eye']
