UNASSIGNED: Fowl adenovirus-4 is an infectious agent causing @entity751 in @entity1573 . Adenovirus are non-enveloped virions having linear, double stranded DNA. Viral genome codes for few structural and non structural proteins. 100K is an important non-structural viral protein. Open reading frame for coding sequence of 100K protein was cloned with oligo @entity3793 tag and expressed in @entity526 as a fusion protein. Nucleotide sequence of the gene revealed that 100K gene of FAdV-4 has high homology (98%) with the respective gene of FAdV-10. Recombinant 100K protein was expressed in @entity526 and purified by @entity3406 affinity chromatography. Immunization of @entity1573 with recombinant 100K protein elicited significant serum antibody titers. However challenge protection test revealed that 100K protein conferred little protection (40%) to the immunized @entity1573 against pathogenic viral challenge. So it was concluded that 100K gene has 2397  bp length and recombinant 100K protein has molecular weight of 95  kDa. It was also found that the recombinant protein has little capacity to affect the immune response because in-spite of having an important role in intracellular transport _ folding of viral capsid proteins during viral replication, it is not exposed on the surface of the virus at any stage.

Molecular cloning, expression and characterization of 100K gene of fowl adenovirus-4 for prevention and control of XXXX .

@entity3406 :: ('MESH:D009532', 'Chemical') :: ['nickel'] @entity3793 :: ('CHEBI:27570', 'Chemical') :: ['histidine'] @entity1573 :: ('9031', 'Species') :: ['chickens', 'chicken'] @entity751 :: ('MESH:D013577', 'Disease') :: ['Hydropericardium syndrome'] @entity526 :: ('562', 'Species') :: ['Escherichia coli', 'E.  coli']
@entity751 :: (MESH:D013577,Disease) :: ['hydropericardium syndrome']
