Mesenchymal stem cells (MSCs), precursors of diverse stromal cells, can support hematopoiesis in vitro and can promote the implantation of hematopoietic stem cells in vivo when co-transplanted with @entity22416 (+) cells. The aim of this study was to investigate the potential effect of MSCs on the hematopoietic development of embryonic stem cells ( @entity2828 cells) and the feasibility of a novel system in which @entity2828 cells will be co-cultured with MSCs. The @entity19 bone marrow MSCs were isolated and cultured and then their phenotype and differentiation function were identified with FCM and histochemical technique. The CCE cells, @entity19 @entity2828 cell line, were co-cultured with the isolated MSCs and the hematopoietic differentiation of CCE cells was observed with hematopoietic clonogenic assay and RT-PCR. The results showed that the morphology of MSCs became gradually homogeneous with the passage culture of cells. After passage 4, the marker of @entity17776 , @entity27064 , @entity3844 and @entity31973 were highly expressed, however, @entity22416 and @entity8236 , the specific marker of hematopoietic and endothelial cells, could hardly be identified. The isolated MSCs differentiated into adipocytes and osteoblasts in specific induction culture system. After maintaining culture on @entity19 embryonic fibroblasts, CCE cells were plated in suspended culture system with only differentiation inductive agents and co-culture system in which MSCs were added. Compared with CCE cell suspended culture, the cells differentiated into embryoid body were obviously enhanced and there were no colony-forming cells in the co-culture system of @entity2828 cells and MSCs. In addition, transcription factor @entity7002 in co-cultured CCE cells was expressed and hematopoietic markers, @entity11132 , @entity34579 and @entity69732 , were negative. The ability of embryoid bodies derived from the co-culture system to produce hematopoietic colonies was markedly higher than that from the suspended culture system. It is concluded that MSCs inhibit the initial differentiation of ESC and enhance hematopoietic differentiation ability of the co-cultured @entity2828 cells.

[Effect of bone marrow mesenchymal stem cells on hematopoietic differentiation of XXXX embryonic stem cells].

@entity8236 :: ('19264(Tax:10090)', 'Gene') :: ['CD45'] @entity2828 :: ('MESH:D012512', 'Disease') :: ['ES'] @entity17776 :: ('20238(Tax:10090)', 'Gene') :: ['Sca-1'] @entity11132 :: ('16542(Tax:10090)', 'Gene') :: ['Flk-1'] @entity34579 :: ('14460(Tax:10090)', 'Gene') :: ['GATA-1'] @entity19 :: ('10090', 'Species') :: ['mouse', 'murine'] @entity31973 :: ('13805(Tax:10090)', 'Gene') :: ['CD105'] @entity69732 :: ('15132(Tax:10090)', 'Gene') :: ['beta-H1'] @entity27064 :: ('16412(Tax:10090)', 'Gene') :: ['CD29'] @entity7002 :: ('100846986', 'Gene') :: ['Oct-4'] @entity3844 :: ('12505(Tax:10090)', 'Gene') :: ['CD44'] @entity22416 :: ('12490(Tax:10090)', 'Gene') :: ['CD34']
@entity19 :: (10090,Species) :: ['murine']
