OBJECTIVE: To describe the epithelial healing rates observed in freshly cultured @entity95 corneas chemically burned with high-concentration @entity5836 ( @entity5828 ) and @entity7159 ( @entity6674 ) and subsequently treated with phototherapeutic keratectomy (PTK). METHODS: We obtained 126 fresh @entity14726 from cadaveric New Zealand white @entity95 . Each @entity267 was exposed to 4-mm cellulose sponges soaked in a solution of topical 0.9% isotonic @entity2827 solution, 2M @entity5828 , or 0.5M @entity6674 . A transepithelial PTK (6-mm zone; 100-microm ablation depth) was then performed using the excimer laser (150-mJ/cm(2) energy pulse; 20 nanosecond duration; and 10-Hz frequency). Corneas were placed in tissue culture, and 1 @entity267 from each group was taken out of culture each day after treatment. Re-epithelialization was monitored by means of @entity941 staining, slitlamp photography, and histopathological analysis. RESULTS: Corneas treated with @entity5828 and @entity6674 exhibited immediate epithelial defects that slowly healed over time. In PTK-treated corneas, the re-epithelialization rate was accelerated compared with that of controls (P =.003 for the @entity5828 group, and P<.001 for the @entity6674 group). The new epithelial layers were smoother in PTK-treated corneas, as confirmed by results of histopathological analysis. CONCLUSION: @entity16 caused by @entity5828 and @entity6674 may be modulated in vitro by PTK in this @entity95 model. CLINICAL RELEVANCE: After corneal chemical damage, 193-nm excimer laser PTK accelerates epithelial wound healing.

Re-epithelialization in XXXX organ culture after chemical burns and excimer laser treatment.

@entity14726 :: ('MESH:C536816', 'Disease') :: ['corneoscleral rims'] @entity267 :: ('MESH:D065306', 'Disease') :: ['cornea'] @entity5836 :: ('MESH:D006851', 'Chemical') :: ['hydrochloric acid'] @entity16 :: ('MESH:D009422', 'Disease') :: ['Corneal damage'] @entity95 :: ('9986', 'Species') :: ['rabbits', 'rabbit'] @entity5828 :: ('CHEBI:17883', 'Chemical') :: ['HCl'] @entity6674 :: ('CHEBI:32145', 'Chemical') :: ['NaOH'] @entity941 :: ('MESH:D019793', 'Chemical') :: ['fluorescein'] @entity2827 :: ('MESH:D012965', 'Chemical') :: ['sodium chloride'] @entity7159 :: ('MESH:D012972', 'Chemical') :: ['sodium hydroxide']
@entity267 :: (MESH:D065306,Disease) :: ['cornea']
