Rapid osseointegration is recognized as a critical factor in determining the success rate of orthopedic and dental implants. Microarc oxidation (MAO) fabricated @entity6701 coatings with a porous topography have been proven to be a potent approach to enhance osteogenic capacity. Now we report two kinds of new @entity8038 with similar micromorphologies but different nanotopographies (i.e., MAO and MAO-AK coatings), and both coatings significantly promote cell attachment and osteogenic differentiation through mediating the integrin b1 signaling pathway. In this study, @entity702 with a unique hierarchical micro/nanomorphology surface was fabricated by a novel duplex coating process, that is, the first a @entity6701 layer was coated by MAO, and then the coating was electrochemically reduced in alkaline solution (MAO-AK). A series of in vitro stem cell differentiation and in vivo osseointegration experiments were carried out to evaluate the osteogenic capacity of the resulting coatings. In vitro, the initial adhesion of the @entity235 bone marrow stem cells (BMSCs) seeded on the MAO and MAO-AK coatings was significantly enhanced, and cell proliferation was promoted. In addition, the expression levels of osteogenesis-related genes, osteorix, alkaline @entity6546 (ALP), osteopontin, and osteocalcin, in the @entity235 BMSCs, were all up-regulated after incubation on these coatings, especially on the MAO-AK coating. Also, the in vitro ALP activity and mineralization capacity of @entity235 BMSC cultured on the MAO-AK group was better than that on the MAO group. Furthermore, 6 weeks after insertion of the @entity702 implants into @entity235 femurs, both the bone formation speed and the bone-implant contact ratio of the MAO-AK group were significantly higher than those of the MAO group. All these results suggest that this duplex coating process is promising for engineering @entity702 surfaces to promote osseointegration for dental and orthopedic applications.

Enhanced Osseointegration of Hierarchical Micro/Nanotopographic XXXX Fabricated by Microarc Oxidation and Electrochemical Treatment.

@entity6546 :: ('MESH:D010710', 'Chemical') :: ['phosphates'] @entity235 :: ('9615', 'Species') :: ['canine'] @entity8038 :: ('MESH:D058456', 'Disease') :: ['hierarchical coatings'] @entity6701 :: ('MESH:C009495', 'Chemical') :: ['titanium oxide'] @entity702 :: ('MESH:D014025', 'Chemical') :: ['titanium']
@entity702 :: (MESH:D014025,Chemical) :: ['Titanium']
