UNASSIGNED: Obesity is a growing health problem in @entity1 as well as companion animals. In the development and progression of @entity28 -associated diseases, the members of the renin- @entity7774 system (RAS) are proposed to be involved. Particularly, the prevalence of @entity6 in @entity468 has increased enormously which is often been linked to @entity28 as well as to RAS. So far, reports about the expression of a local RAS in cat adipocytes are missing. Therefore, we investigated the mRNA expression of various RAS genes as well as the adipocyte marker genes adiponectin, leptin and PPAR-y in feline adipocytes using quantitative PCR. To characterize the gene expression during adipogenesis, feline pre-adipocytes were differentiated into adipocytes in a primary cell culture and the expression of RAS key genes measured. All major RAS components were expressed in feline cells, but obvious differences in the expression between pre-adipocytes and the various differentiation stages were found. Interestingly, the two enzymes @entity57388 and @entity68834 showed an opposite expression course. In addition to the in  vitro experiments, mature adipocytes were isolated from subcutaneous and visceral adipose tissue. Significant differences between both fat depots were found for @entity57388 as well as AT1 receptor with greater expression in subcutaneous than in visceral adipocytes. Visceral adipocytes had significantly higher adiponectin and PPAR-y mRNA level compared to the subcutaneous fat cells. Concerning the nutritional status, a significant lower expression of @entity68834 was measured in subcutaneous adipocytes of overweight @entity468 . In summary, the results show the existence of a potentially functional local RAS in feline adipose tissue which is differentially regulated during adipogenesis and dependent on the fat tissue depot and nutritional status. These findings are relevant for understanding the development of @entity28 -associated diseases in @entity468 such as @entity6 .

Characterization of key genes of the renin- XXXX system in mature feline adipocytes and during in  vitro adipogenesis.

@entity1 :: ('9606', 'Species') :: ['humans'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetes mellitus', 'type 2 diabetes mellitus'] @entity28 :: ('MESH:D009765', 'Disease') :: ['obesity'] @entity7774 :: ('MESH:D000809', 'Chemical') :: ['angiotensin'] @entity68834 :: ('554349(Tax:9685)', 'Gene') :: ['ACE2'] @entity57388 :: ('101094061', 'Gene') :: ['ACE'] @entity468 :: ('9685', 'Species') :: ['cats']
@entity7774 :: (MESH:D000809,Chemical) :: ['angiotensin']
