UNASSIGNED: Diatoms are eukaryotic microalgae that are responsible for up to 40% of the ocean's primary productivity. How diatoms respond to environmental perturbations such as elevated @entity505 concentrations in the atmosphere is currently poorly understood. We developed a transcriptional regulatory network based on various transcriptome sequencing expression libraries for different environmental responses to gain insight into the marine diatom's metabolic and regulatory interactions and provide a comprehensive framework of responses to increasing atmospheric @entity505 levels. This transcriptional regulatory network was integrated with a recently published genome-scale metabolic model of @entity34466 to explore the connectivity of the regulatory network and shared metabolites. The integrated regulatory and metabolic model revealed highly connected modules within @entity505 and @entity1568 metabolism. @entity34466 's response to rising @entity505 levels was analyzed by using the recent genome-scale metabolic model with cross comparison to experimental manipulations of @entity544 . IMPORTANCE Using a systems biology approach, we studied the response of the marine diatom @entity34466 to changing atmospheric @entity505 concentrations on an ocean-wide scale. By integrating an available genome-scale metabolic model and a newly developed transcriptional regulatory network inferred from transcriptome sequencing expression data, we demonstrate that @entity505 metabolism and @entity1568 metabolism are strongly connected and the genes involved are coregulated in this model diatom. These tight regulatory constraints could play a major role during the adaptation of @entity34466 to increasing @entity505 levels. The transcriptional regulatory network developed can be further used to study the effects of different environmental perturbations on @entity34466 's metabolism.

Integrated Regulatory and Metabolic Networks of the Marine Diatom XXXX Predict the Response to Rising CO2 Levels.

@entity505 :: ('MESH:D002244', 'Chemical') :: ['carbon'] @entity1568 :: ('MESH:D009584', 'Chemical') :: ['nitrogen'] @entity544 :: ('MESH:D002245', 'Chemical') :: ['carbon dioxide'] @entity34466 :: ('2850', 'Species') :: ['Phaeodactylum tricornutum', 'P.  tricornutum']
@entity34466 :: (2850,Species) :: ['Phaeodactylum tricornutum']
