@entity6835 -like toxin (SLT) has been implicated in the pathogenesis of @entity1381 and its attendant endothelial cell (EC) injury. Key serotypes of @entity526 produce @entity27173 in addition to another highly pro-inflammatory molecule, lipopolysaccharide (LPS). It has previously been established that @entity27173 induces EC apoptosis and that LPS enhances this effect. LPS alone has no affect on @entity1 EC viability, and the mechanism for this enhancement remains unknown. In the present report, we demonstrate that @entity27173 sensitizes EC to LPS-induced apoptosis. Pretreatment with @entity27173 sensitized EC to LPS-induced apoptosis, whereas pretreatment with LPS did not influence @entity27173 -induced apoptosis. @entity27173 exposure resulted in decreased expression of @entity1182 ( @entity1182 ), an anti-apoptotic protein that has previously been shown to block LPS-induced apoptosis. This @entity27173 -mediated decrease in @entity1182 expression preceded the onset of apoptosis elicited by @entity27173 alone or in combination with LPS. @entity27173 -mediated decrements in @entity1182 expression correlated in a dose- and time-dependent manner with sensitization to LPS-induced apoptosis. Finally, transient or stable overexpression of @entity1182 protected against LPS enhancement of @entity27173 -induced apoptosis, and this protection corresponded with sustained expression of @entity1182 . Together, these data suggest that @entity27173 sensitizes EC to LPS-induced apoptosis by inhibiting @entity1182 expression.

XXXX -like toxin inhibition of @entity1182 expression sensitizes endothelial cells to bacterial lipopolysaccharide-induced apoptosis.

@entity6835 :: ('MESH:D004405', 'Disease') :: ['Shiga'] @entity1 :: ('9606', 'Species') :: ['human'] @entity1182 :: ('8837', 'Gene') :: ['FLICE-like inhibitory protein', 'FLIP'] @entity27173 :: ('55850', 'Gene') :: ['SLT-1'] @entity1381 :: ('MESH:D006463', 'Disease') :: ['hemolytic uremic syndrome'] @entity526 :: ('562', 'Species') :: ['Escherichia coli']
@entity6835 :: (MESH:D004405,Disease) :: ['Shiga']
