BACKGROUND: MicroRNAs (miR, miRNAs) play pivotal roles in numerous physiological and pathophysiological contexts. We investigated whether miR-362-5p act as an oncogene in @entity1860 ( @entity1860 ) and aimed to understand its potential underlying mechanisms. METHODS: We compared the miR-362-5p expression levels between @entity1860 and non- @entity1860 cell lines, and between fresh blood samples from @entity1860 @entity1 and normal healthy controls using quantitative real-time PCR (qPCR). Cell counting kit-8 (CCK-8) and @entity4404 - @entity2823 /PI analyses were used to measure the effects of miR-362-5p on proliferation and apoptosis, and Transwell assays were used to evaluate migration and invasion. A xenograft model was used to examine in vivo tumourigenicity. The potential target of miR-362-5p was confirmed by a luciferase reporter assay, qPCR and western blotting. Involvement of the @entity10886 and P38 pathways was investigated by western blotting. RESULTS: miR-362-5p was up-regulated in @entity1860 cell lines and fresh blood samples from @entity1860 @entity1 , and was associated with @entity851 and DNA damage-inducible (GADD)45a down-regulation. Inhibition of miR-362-5p simultaneously repressed @entity5 growth and up-regulated @entity10187 expression in a xenograft model. Consistently, the knockdown of @entity10187 expression partially neutralized the effects of miR-362-5p inhibition. Furthermore study suggested that @entity10187 mediated downstream the effects of miR-362-5p, which might indirectly regulates the activation of the @entity10886 and P38 signalling pathways. CONCLUSION: miR-362-5p acts as an oncomiR that down-regulates @entity10187 , which consequently activates the @entity10886 and P38 signalling. This finding provides novel insights into @entity1860 leukaemogenesis and may help identify new diagnostic and therapeutic targets.

MiR-362-5p promotes the malignancy of chronic myelocytic leukaemia via down-regulation of XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity851 :: ('MESH:D006130', 'Disease') :: ['Growth arrest'] @entity4404 :: ('308', 'Gene') :: ['Annexin V'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumour'] @entity10187 :: ('1647', 'Gene') :: ['GADD45a'] @entity2823 :: ('MESH:D016650', 'Chemical') :: ['FITC'] @entity1860 :: ('MESH:D015464', 'Disease') :: ['CML', 'chronic myeloid leukaemia'] @entity10886 :: ('5599;5601', 'Gene') :: ['JNK1/2']
@entity10187 :: (1647,Gene) :: ['GADD45a']
