A @entity291 plasminogen activator of atypical molecular mass ( approximately 45 kDa) from @entity8211 SK880 had been identified previously (L. B. Johnsen, K. Poulsen, M. Kilian, and T. E. Petersen. Infect. Immun. 67:1072-1078, 1999). The strain was isolated from a clinical case of @entity291 @entity1271 . The isolate was found not to secrete PauA, a @entity291 plasminogen activator expressed by the majority of @entity8211 strains. Analysis of the locus normally occupied by pauA revealed an absence of the pauA open reading frame. However, an alternative open reading frame was identified within the same locus. Sequence analysis of the putative gene suggested limited but significant homology to other plasminogen activators. A candidate signal peptide sequence and cleavage site were also identified. Expression cloning of DNA encoding the predicted mature protein (lacking signal peptide) confirmed that the open reading frame encoded a plasminogen activator of the expected size, which we have named PauB. Both native and recombinant forms of PauB displayed an unexpectedly broad specificity profile for @entity291 , ovine, @entity1641 , @entity36323 , porcine, @entity95 , and @entity1 plasminogen. Clinical and nonclinical field isolates from nine United Kingdom sites were screened for the pauB gene and none were identified as carrying it. Similarly, clinical isolates from 20 Danish herds were all found to encode PauA and not PauB. Therefore, PauB represents a novel but rare bacterial plasminogen activator which displays very broad specificity.

Characterization of PauB, a novel broad-spectrum plasminogen activator from XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity1641 :: ('9796', 'Species') :: ['equine'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity36323 :: ('CHEBI:44556', 'Chemical') :: ['caprine'] @entity8211 :: ('1349', 'Species') :: ['S. uberis', 'Streptococcus uberis strain'] @entity1271 :: ('MESH:D008413', 'Disease') :: ['mastitis'] @entity291 :: ('9913', 'Species') :: ['bovine']
@entity8211 :: (1349,Species) :: ['Streptococcus uberis']
