BACKGROUND: Faecalibacterium prausnitzii comprises 2 phylogroups, whose abundance in healthy and diseased gut and in conjunction with @entity526 has not yet been studied. This work aims to determine the contribution of F. prausnitzii phylogroups I and II in @entity1944 and to assess their potential diagnostic usefulness as biomarkers for gut diseases. METHODS: Total F. prausnitzii, its phylogroups, and @entity526 loads were determined by quantitative polymerase chain reaction targeting the 16S rRNA gene on biopsies from 31 healthy controls (H), 45 @entity1 with @entity30 ( @entity30 ), 25 @entity1 with @entity31 , 10 @entity1 with @entity1744 , and 20 @entity1 with @entity14 . Data were normalized to total bacterial counts and analyzed according to @entity1 ' disease location and clinical characteristics. RESULTS: Lower levels of both total F. prausnitzii and phylogroup I were found in subjects with @entity30 , @entity31 , and @entity14 (P < 0.001) compared with H subjects. Phylogroup I load was a better biomarker than total F. prausnitzii to discriminate subjects with gut disorders from H. Phylogroup II depletion was observed only in @entity1 with @entity30 (P < 0.001) and can be potentially applied to differentiate @entity1066 from @entity30 . No statistically significant correlation between @entity526 and any of the 2 F. prausnitzii phylogroups was found in any group of @entity1 or by @entity29 location. Phylogroup I was lower in active @entity1 with @entity30 , whereas those @entity30 with intestinal resection showed a reduction in phylogroup II. Treatments with @entity2983 and immunosuppressants did not result in the recovery of F. prausnitzii phylogroups abundance. CONCLUSIONS: F. prausnitzii phylogroup I was depleted in @entity30 , @entity31 , and @entity14 , whereas phylogroup II was specifically reduced in @entity30 . Quantification of F. prausnitzii phylogroups and @entity526 may help to identify gut disorders and to classify @entity29 location.

Changes in the Abundance of Faecalibacterium prausnitzii Phylogroups I and II in the Intestinal Mucosa of @entity29 and XXXX with @entity14 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity1944 :: ('MESH:D007410', 'Disease') :: ['intestinal disease'] @entity1066 :: ('MESH:D014456', 'Disease') :: ['ulcerative pancolitis'] @entity31 :: ('MESH:D003093', 'Disease') :: ['ulcerative colitis'] @entity29 :: ('MESH:D015212', 'Disease') :: ['inflammatory bowel disease'] @entity1744 :: ('MESH:D043183', 'Disease') :: ['irritable bowel syndrome'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal cancer'] @entity30 :: ('MESH:D003424', 'Disease') :: ['CD', 'colonic CD', "Crohn's disease"] @entity2983 :: ('MESH:D019804', 'Chemical') :: ['mesalazine'] @entity526 :: ('562', 'Species') :: ['Escherichia coli', 'E. coli']
@entity1 :: (9606,Species) :: ['Patients']
