The @entity19 autosomal deleted in @entity3893 -like protein ( @entity32208 ) is a germ cell-restricted RNA-binding protein essential for sperm production. Homozygous disruption of the @entity32208 gene results in the absence of germ cells beyond the spermatogonial stage. Progress into the function of @entity32208 in spermatogenesis has been hampered without identification of the cognate mRNA substrates that it binds to and regulates. Using the isolation of specific nucleic acids associated with proteins (SNAAP) technique recently developed in our lab, we identified mRNAs from testis that were specifically bound by @entity32208 . One mRNA encoded the @entity69655 protein, a testicular cell adhesion protein essential for the progression of spermatogenesis. A 26-nucleotide region necessary and sufficient to bind @entity32208 was found within additional mRNAs isolated by the screen. These included mRNA encoding @entity69656 , a protein associated with @entity18122 ; @entity69657 , an mRNA-binding protein involved in translation activation, and @entity69658 , a TATA box-binding protein-like protein involved in transcriptional regulation. Each mRNA containing the @entity32208 binding site was specifically bound by @entity32208 . A similar sequence is also present in the @entity36065 mRNA, a @entity1889 / @entity669 phosphatase involved in cell cycle progression. The @entity32208 and @entity36065 homologues are functionally linked in @entity238 and are necessary for spermatogenesis. Our demonstration that @entity69655 and @entity36065 mRNAs are bound by @entity32208 suggests that @entity32208 regulates a subset of mRNAs necessary for germ cell development and cell cycle progression. Understanding how @entity32208 regulates the target mRNAs will provide new insights into spermatogenesis, strategies for therapeutic intervention in azoospermic @entity1 , and novel approaches for male contraception.

Identification of target messenger RNA substrates for the XXXX deleted in @entity3893 -like RNA-binding protein.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity18122 :: ('17869(Tax:10090)', 'Gene') :: ['myc'] @entity69656 :: ('18484(Tax:10090)', 'Gene') :: ['Pam'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity32208 :: ('13164(Tax:10090)', 'Gene') :: ['DAZL', 'mDAZL'] @entity238 :: ('7227', 'Species') :: ['Drosophila'] @entity69655 :: ('22024(Tax:10090)', 'Gene') :: ['Tpx-1'] @entity69657 :: ('231413(Tax:10090)', 'Gene') :: ['GRSF1'] @entity1889 :: ('CHEBI:26986', 'Chemical') :: ['threonine'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine'] @entity69658 :: ('237336(Tax:10090)', 'Gene') :: ['TRF2'] @entity36065 :: ('12530(Tax:10090)', 'Gene') :: ['Cdc25A'] @entity3893 :: ('MESH:D053713', 'Disease') :: ['azoospermia']
@entity19 :: (10090,Species) :: ['murine']
