Surgical resection is thought to be the best treatment for @entity96 , including @entity157 and metastatic @entity96 if there are a small number of @entity5 . @entity96 is one of the main causes of @entity204 from @entity5 worldwide. The prognosis of @entity96 is still poor. Mutation of @entity10740 , which is well known as a @entity5 suppressor gene, is observed in many cases of advanced @entity96 . @entity5 gene therapy using @entity10740 , which transduces the wild-type @entity10740 gene in the @entity5 , is a promising new strategy for treating @entity96 . Selective and less invasive gene delivery to the @entity96 is necessary for clinical @entity96 gene therapy. The first purpose of the current study was to determine the best way to deliver the gene of interest to the @entity96 selectively. The second purpose was to study the @entity5 suppressive effect of intrahepatic arterial injection of an adenovirus vector with the @entity10740 gene (AdCMV- @entity10740 ), followed by administration of CDDP and noting its side effects. We injected AdCMV-LacZ via hepatic arteries of @entity35 bearing RCN-9 colon @entity5 @entity3 in the liver. Injection via the hepatic artery resulted in more successful gene transduction to the @entity96 in a @entity5 -selective manner than did injection via the portal vein. At 48 hrs after arterial injection of AdCMV- @entity10740 , CDDP (3 mg/kg) was administered in the peritoneal cavity of each @entity35 . The use of CDDP with arterial injection of AdCMV- @entity10740 resulted in more extensive apoptosis in the @entity35 @entity96 without any deterioration in liver function. In conclusion, hepatic arterial injection of an adenovirus vector is better than portal vein injection for gene transduction efficiency, and causes no liver function disorder even when the injection is combined with CDDP.

Safety and growth suppressive effect of intra-hepatic arterial injection of AdCMV- XXXX combined with CDDP to @entity35 liver @entity3 .

@entity10740 :: ('301300(Tax:10116)', 'Gene') :: ['p53'] @entity96 :: ('MESH:D008113', 'Disease') :: ['liver tumor', 'liver tumors', 'Liver carcinoma', 'liver carcinoma'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'Cancer', 'tumors'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity157 :: ('MESH:D006528', 'Disease') :: ['hepatocellular carcinoma'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death']
@entity10740 :: (301300(Tax:10116),Gene) :: ['p53']
