Exposure to ultraviolet (UV) irradiation has detrimental effects on skin accompanied by the increased metabolism of hyaluronan (HA), a linear polysaccharide important for the normal physiological functions of skin. In this study, the modulation of @entity1 keratinocyte response to UVB irradiation by HA (970 kDa) was investigated. Immortalized @entity1 keratinocytes (HaCaT) were irradiated by a single dose of UVB and immediately treated with HA for 6 and 24 h. The irradiation induced a significant decrease in the gene expression of @entity1759 and @entity2938 6 h after irradiation. The expressions of other HA receptors, including toll-like receptor 4 and the receptor for HA-mediated motility, were not detected in either the control or UVB-irradiated or HA-treated HaCaT cells. UVB irradiation induced a significant decrease in the gene expression of @entity4660 and @entity25896 6 h after irradiation. The expressions of @entity9907 and hyaluronidase-3 were not significantly modulated by UV irradiation. Interestingly, HA treatment did not significantly modulate any of these effects. In contrast, HA significantly suppressed UVB-induced pro-inflammatory cytokine release including @entity650 and @entity687 . Similarly, HA treatment reduced the UVB-mediated production of transforming growth factor b1. HA treatment also significantly reduced the UV irradiation-mediated release of soluble @entity1759 into the media. Finally, HA partially, but significantly, suppressed the UVB-induced decrease in cell viability. Data indicate that HA had significant protective effects for HaCaT cells against UVB irradiation.

Hyaluronan minimizes effects of UV irradiation on XXXX keratinocytes.

@entity1 :: ('9606', 'Species') :: ['human'] @entity650 :: ('3569', 'Gene') :: ['interleukin-6'] @entity25896 :: ('8692', 'Gene') :: ['hyaluronidase-2'] @entity4660 :: ('3037', 'Gene') :: ['HA synthase-2'] @entity1759 :: ('960', 'Gene') :: ['CD44'] @entity2938 :: ('7097', 'Gene') :: ['toll-like receptor 2'] @entity687 :: ('3576', 'Gene') :: ['interleukin-8'] @entity9907 :: ('3038', 'Gene') :: ['HA synthase-3']
@entity1 :: (9606,Species) :: ['human']
