@entity31103 are potentially important mediators of biological processes including vascular function, apoptosis, and @entity715 . Recent studies indicate that the concentrations of @entity31103 in the plasma from healthy individuals are lower than previously reported and in the range of 30-120 nM. The mechanisms of formation and metabolism of these low nM concentrations, capable of exerting biological effects, remain unknown. An important issue that remains unresolved is the significance of the reactions of low fluxes of @entity842 (NO) with @entity26 to form @entity31103 in a complex biological medium such as plasma, and the impact of red blood cells on the formation of @entity31103 in blood. These issues were addressed by exposing plasma to varying fluxes of NO and measuring the net formation of @entity31103 . In the presence of @entity26 and physiological fluxes of NO, the predominant @entity31103 formed is @entity10739 at concentrations in the high nM range (approximately 400-1000 nM). Although the formation of @entity31103 by NO was attenuated in whole blood, presumably by erythrocytic hemoglobin, significant amounts of @entity31103 within the physiological range of @entity31103 concentrations (approximately 80 nM) were still formed at physiological fluxes of NO. Little is known about the stability of @entity10739 in plasma, and this is central to our understanding of the biological effectiveness of @entity31103 . Low molecular weight @entity1433 decreased the half-life of @entity10739 in plasma, and the stability of @entity10739 is enhanced by the alkylation of free @entity1433 . Our data suggests that physiologically relevant concentrations of @entity31103 can be formed in blood through the reaction of NO with @entity26 and proteins, despite the low rates of reaction of @entity26 with NO and the presence of erythrocytes.

Formation of nanomolar concentrations of @entity10739 in human plasma by XXXX .

@entity1433 :: ('MESH:D013438', 'Chemical') :: ['thiols'] @entity10739 :: ('MESH:C091665', 'Chemical') :: ['S-nitroso-albumin'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity31103 :: ('MESH:D026403', 'Chemical') :: ['S-nitrosothiols', 'S-Nitrosothiols', 'S-nitrosothiol'] @entity715 :: ('MESH:D013927', 'Disease') :: ['thrombosis']
@entity842 :: (MESH:D009569,Chemical) :: ['nitric oxide']
