Elongation factor subunit eEF1Bbeta (formerly EF-1beta in plants and EF- @entity69447 in animals) was identified and cloned in a screen for proteins from @entity3587 that interact with a cyclin-dependent kinase (CDK). CDKs are enzymes that regulate progression through meiotic and mitotic cell cycles in eukaryotes. eEF1Bbeta and the related protein eEF1Balpha (formerly EF-1beta' in plants and EF-1beta in animals and fungi) can catalyze @entity3007 / @entity3006 exchange on the G-protein eEF1A (formerly EF-1alpha in plants, animals and fungi) during the elongation phase of protein synthesis in eukaryotes. Recombinant Cdc2 and its native homologues from @entity3587 extracts associated both in vitro and in vivo with eEF1Bbeta. A Cdc2-cyclin B complex phosphorylated recombinant plant eEF1Bbetas, but not eEF1Balpha. These interactions between CDK and eEF1Bbeta prompted investigations into the in vivo consequences of this relationship. Expression of cDNAs encoding @entity579 or @entity3587 eEF1Bbeta subunits failed to complement a @entity375 mutant deleted for the eEF1Balpha gene, as was previously observed for the @entity1 eEF1Bbeta. However, replacement of Thr91, the sole consensus CDK phosphorylation site in @entity3587 eEF1Bbeta, with @entity493 allowed the @entity3587 protein to substitute for eEF1Balpha function in vivo. In addition, this rescued strain was severely cold sensitive, and more sensitive to translational inhibitors than wild-type @entity375 . Taken together, these results suggest a physiological connection between the cyclin-dependent class of kinases and a translational elongation factor in mitotic cells, and provide the first in vivo evidence that an altered form of eEF1Bbeta can serve as the @entity6370 exchange factor for eEF1A.

Mutation of a conserved CDK site converts a metazoan Elongation Factor 1Bbeta subunit into a replacement for XXXX eEF1Balpha.

@entity3007 :: ('MESH:D006160', 'Chemical') :: ['GTP'] @entity1 :: ('9606', 'Species') :: ['human'] @entity6370 :: ('CHEBI:61292', 'Chemical') :: ['guanine nucleotide'] @entity69447 :: ('1delta', 'DNAMutation') :: ['1delta'] @entity3006 :: ('MESH:D006153', 'Chemical') :: ['GDP'] @entity375 :: ('4932', 'Species') :: ['yeast', 'Saccharomyces cerevisiae'] @entity579 :: ('4530', 'Species') :: ['rice'] @entity493 :: ('CHEBI:16449', 'Chemical') :: ['alanine'] @entity3587 :: ('3888', 'Species') :: ['pea']
@entity375 :: (4932,Species) :: ['yeast']
