The regular array of distally pointing hairs on the mature @entity238 wing is evidence for the fine control of Planar Cell Polarity ( @entity212 ) during wing development. Normal wing @entity212 requires both the Frizzled (Fz) @entity212 pathway and the Fat/Dachsous (Ft/Ds) pathway, although the functional relationship between these pathways remains under debate. There is strong evidence that the Fz @entity212 pathway signals twice during wing development, and we have previously presented a Bidirectional-Biphasic Fz @entity212 signaling model which proposes that the Early and Late Fz @entity212 signals are in different directions and employ different isoforms of the @entity42944 protein. The goal of this study was to investigate the role of the Ft/Ds pathway in the context of our Fz @entity212 signaling model. Our results allow us to draw the following conclusions: (1) The Early Fz @entity212 signals are in opposing directions in the anterior and posterior wing and converge precisely at the site of the L3 wing vein. (2) Increased or decreased expression of Ft/Ds pathway genes can alter the direction of the Early Fz @entity212 signal without affecting the Late Fz @entity212 signal. (3) Lowfat, a Ft/Ds pathway regulator, is required for the normal orientation of the Early Fz @entity212 signal but not the Late Fz @entity212 signal. (4) At the time of the Early Fz @entity212 signal there are symmetric gradients of dachsous (ds) expression centered on the L3 wing vein, suggesting Ds activity gradients may orient the Fz signal. (5) Localized knockdown or over-expression of Ft/Ds pathway genes shows that boundaries/gradients of Ft/Ds pathway gene expression can redirect the Early Fz @entity212 signal specifically. (6) Altering the timing of ds knockdown during wing development can separate the role of the Ft/Ds pathway in wing morphogenesis from its role in Early Fz @entity212 signaling.

Two frizzled planar cell polarity signals in the XXXX wing are differentially organized by the Fat/Dachsous pathway.

@entity42944 :: ('45343(Tax:7227)', 'Gene') :: ['Prickle'] @entity212 :: ('CHEBI:8058', 'Chemical') :: ['PCP'] @entity238 :: ('7227', 'Species') :: ['Drosophila']
@entity238 :: (7227,Species) :: ['Drosophila']
