Continuous administration of @entity1176 analogs results in an inhibition of @entity5 growth that may be mediated in part by direct activation of @entity1176 receptors (GnRHRs) expressed on @entity5 cells. However, it is not fully understood how the @entity23042 mediates these growth effects. This study aimed to determine how the presence or absence of this receptor in different cell types might affect the ability of @entity1176 to directly mediate growth effects. We demonstrate that continuous treatment with @entity1176 or a @entity1176 agonist ( @entity4397 ) induces an antiproliferative effect in a gonadotrope-derived cell line (LbetaT2) and also in HEK293 cells stably expressing either the @entity35 or @entity1 @entity23042 . The antiproliferative effect was time and dose dependent and was verified using @entity665 incorporation, light microscopy, and analysis of cell number. Inhibition was specifically mediated via the @entity23042 , as cotreatment of the @entity23042 -expressing cell lines with a @entity1176 antagonist blocked the growth-suppressive effect induced by @entity4397 treatment. Cell cycle analysis revealed that @entity4397 -treated HEK/ @entity23042 cell lines induced an accumulation of cells in the G2/M phase, whereas a G0/G1 arrest was observed in LbetaT2 cells. @entity4397 treatment also caused a small, but significant, increase in apoptotic cells. This study provides evidence for a direct role for the @entity23042 in mediating antiproliferative events in two cell systems, neither of which was derived from extrapituitary reproductive @entity5 . The ability to induce these effects, regardless of the cell system involved, has implications regarding the use of @entity1176 analogs for the treatment of @entity4903 and @entity5 .

XXXX -mediated growth suppression of immortalized LbetaT2 gonadotrope and stable HEK293 cell lines.

@entity1 :: ('9606', 'Species') :: ['human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity665 :: ('CHEBI:53526', 'Chemical') :: ['[3H]thymidine'] @entity4397 :: ('CHEBI:63533', 'Chemical') :: ['GnRHA'] @entity1176 :: ('MESH:D007987', 'Chemical') :: ['GnRH'] @entity23042 :: ('2798', 'Gene') :: ['GnRHR'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity4903 :: ('MESH:D004700', 'Disease') :: ['endocrine-related disorders']
@entity23042 :: (2798,Gene) :: ['Gonadotropin-releasing hormone receptor']
