1. The detergent @entity6000 activates @entity858 glucuronyltransferase from @entity35 liver in vitro six- to seven-fold with @entity11921 as substrate. The enzyme activity when measured in the presence of @entity6000 is increased significantly by pretreatment of male @entity35 with @entity109 for 4 days (90mg/kg each day intraperitoneally). If no @entity6000 is applied in vitro such an increase could not be shown. In all further experiments the enzyme activity was measured after activation by @entity6000 . 2. The K(m) of the enzyme for the substrate @entity11921 does not change on @entity109 pretreatment. 3. When the microsomal fraction from the liver of untreated @entity35 is subfractionated on a @entity969 density gradient, 47% of the enzyme activity is recovered in the rough-surfaced microsomal fraction, which also has a higher specific activity than the smooth-surfaced fraction. 4. Of the increase in activity after the @entity109 pretreatment 50% occurs in the smooth-surfaced fraction, 19% in the rough-surfaced fraction and 31% in the fraction located between the smooth- and rough-surfaced microsomal fractions on the sucrose density gradient. 5. The latency of the enzyme in vitro, as shown by the effect of the detergent @entity6000 , is discussed in relation to the proposed heterogeneity of @entity858 glucuronyltransferase.

The effect of @entity109 on the submicrosomal distribution of @entity858 glucuronyltransferase from XXXX liver.

@entity6000 :: ('MESH:D017830', 'Chemical') :: ['Triton X-100'] @entity858 :: ('MESH:D014530', 'Chemical') :: ['UDP'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity109 :: ('MESH:D010634', 'Chemical') :: ['phenobarbital'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity11921 :: ('MESH:C024836', 'Chemical') :: ['p-nitrophenol']
@entity35 :: (10116,Species) :: ['rat']
