UNASSIGNED: We demonstrated that in @entity1 platelets the endocannabinoid @entity9394 ( @entity14512 ) decreased dose- and time-dependently @entity268 intracellular levels. No effect on @entity268 decrease induced by @entity14512 was observed in the presence of the adenylate cyclase inhibitor @entity15404 as well in platelets pretreated with the @entity2871 A2 receptor antagonist, @entity13078 or with @entity117 , inhibitor of @entity1426 metabolism through the cyclooxygenase pathway. An almost complete recovering of @entity268 level was measured in platelets pretreated with the specific inhibitor of phosphodiesterase (PDE) 3A, @entity7604 . In platelets pretreated with @entity2306 or @entity17138 , inhibitors of PI3K/ @entity584 pathway, and with @entity4385 , inhibitor of phospholipase C pathway, only a partial prevention was shown. @entity268 intracellular level depends on synthesis by @entity1920 cyclase and hydrolysis by PDEs. In @entity14512 -stimulated platelets adenylate cyclase activity seems to be unchanged. In contrast PDEs appear to be involved. In particular @entity37158 was specifically activated, as @entity7604 reversed @entity268 reduction by @entity14512 . @entity14512 enhanced @entity37158 activity through its phosphorylation. The PI3K/ @entity584 pathway and @entity6164 participate to this @entity37158 phosphorylation/activation mechanism as it was greatly inhibited by platelet pretreatment with @entity2306 , @entity17138 , @entity4385 , or the @entity6164 specific inhibitor @entity11009 . Taken together these data suggest that @entity14512 potentiates its power of platelet agonist reducing @entity268 intracellular level. J. Cell. Biochem. 9999: 1-10, 2015.    2015 Wiley Periodicals, Inc.

Regulation of @entity268 Intracellular Levels in XXXX Platelets Stimulated by @entity9394 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity2871 :: ('CHEBI:26995', 'Chemical') :: ['thromboxane'] @entity14512 :: ('CHEBI:52392', 'Chemical') :: ['2-AG'] @entity17138 :: ('MESH:C548887', 'Chemical') :: ['MK2206'] @entity9394 :: ('MESH:C094503', 'Chemical') :: ['2-arachidonoylglycerol'] @entity117 :: ('MESH:D001241', 'Chemical') :: ['aspirin'] @entity1920 :: ('CHEBI:16027', 'Chemical') :: ['adenylate'] @entity1426 :: ('MESH:D016718', 'Chemical') :: ['arachidonic acid'] @entity2306 :: ('MESH:C085911', 'Chemical') :: ['LY294002'] @entity13078 :: ('MESH:C045749', 'Chemical') :: ['SQ29548'] @entity584 :: ('207', 'Gene') :: ['AKT'] @entity15404 :: ('MESH:C017759', 'Chemical') :: ['SQ22536'] @entity4385 :: ('MESH:C060229', 'Chemical') :: ['U73122'] @entity37158 :: ('5139', 'Gene') :: ['PDE3A'] @entity11009 :: ('MESH:C070515', 'Chemical') :: ['GF109203X'] @entity6164 :: ('MESH:C566847', 'Disease') :: ['PKC'] @entity7604 :: ('MESH:D020105', 'Chemical') :: ['milrinone']
@entity1 :: (9606,Species) :: ['Human']
