BACKGROUND: Cell therapy, such as hepatocyte transplantation (HTx), is promising for the treatment of metabolic @entity409 or as a bridge to orthotopic liver transplantation in @entity1 with @entity879 . However, one of the limitations of this therapy is the shortage of donors. The present study aims to investigate whether the two-layer method (TLM) of cold preservation with oxygenation improves the viability and activity of hepatocytes from @entity35 donation after @entity204 (DCD) donors compared with results obtained with the University of Wisconsin (UW) solution. Moreover, we evaluated the hepatocyte function after culture or transplantation into the spleen. MATERIALS AND METHODS: We used male @entity35 for this study. The DCD model was induced by phrenotomy after injecting @entity6950 . We assigned @entity35 based on warm @entity63 times of 15 and 30 min to groups S and L, respectively. Each group (n = 5) was then subdivided as follows: (1) group S: not preserved (S/N), preserved by TLM for 3 h (S/TLM3) and 12 h (S/TLM12), and in the UW solution for 3 h (S/UW3) and 12 h (S/UW12), and (2) group L: not preserved (L/N), preserved by TLM for 3 h (L/TLM3) and 12 h (L/TLM12), and in the UW solution for 3 h (L/UW3) and 12 h (L/UW12). The cell viability and function of isolated DCD hepatocytes were analyzed for culture or HTx into the spleen. RESULTS: The viability and @entity855 levels of DCD hepatocytes significantly improved after TLM compared with the values after preservation in cold UW solution in group S/N (p < 0.059). The levels of albumin production and @entity1133 synthesis by hepatocytes after culture were significantly higher in groups S/TLM3 and S/TLM12 than in groups S/UW3 and S/UW12 (p < 0.05), respectively. Further, serum albumin levels after HTx were also markedly higher in groups S/TLM3 and S/TLM12 than in groups S/UW3 and S/UW12. The morphological features revealed that cultured and transplanted hepatocytes remained clearly viable and maintained an expression for specific hepatic function, such as the production of albumin and glycogen. CONCLUSION: This novel method of oxygenated cold preservation of DCD livers can expand the hepatocyte donor pool for HTx and establish a wider application of this developing technique.

Oxygenated Static Preservation of Donation after XXXX Liver Grafts Improves Hepatocyte Viability and Function.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity1133 :: ('MESH:D014508', 'Chemical') :: ['urea'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemia'] @entity879 :: ('MESH:D017114', 'Disease') :: ['fulminant liver failure'] @entity409 :: ('MESH:D008107', 'Disease') :: ['liver diseases'] @entity6950 :: ('MESH:D006493', 'Chemical') :: ['heparin'] @entity35 :: ('10116', 'Species') :: ['rat', 'Sprague-Dawley rats', 'rats'] @entity204 :: ('MESH:D003643', 'Disease') :: ['cardiac death'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity204 :: (MESH:D003643,Disease) :: ['Cardiac Death']
