OBJECTIVES: An increased platelet activation status is present in @entity1 with @entity1468 . Our purpose was (1) to establish by means of flow cytometry whether platelets circulate in an activated state during the first and second trimesters of pregnancy and (2) to establish whether early platelet activation predicts the onset of @entity1468 . STUDY DESIGN: Consecutively, 244 pregnant @entity1 were included in a prospective study design. Platelets in whole blood samples from the pregnant @entity1 in the first trimester, the second trimester, and after delivery were labeled with the following antibodies associated with platelet activation: anti- @entity8729 ( @entity8729 , alpha-granule secretion), anti- @entity5775 (GP53, lysosomal secretion), anti- @entity2279 ( @entity2279 ', platelet endothelial cell adhesion molecule-1). The surface antigen exposure was determined by double-label flow cytometry with anti- @entity27463 (GPIb, a platelet-specific monoclonal glycoprotein) to select platelets and platelet-derived materials. Preeclampsia was defined as a diastolic blood pressure > or = 90 mm Hg and @entity644 > or = 0.3 gm in a 24-hour urine sample (International Society for Study of @entity101 ). RESULTS: Seventeen of 244 @entity1 had @entity1468 (6.9%). Only first-trimester @entity5775 expression had an area under the curve > 0.5 by receiver-operator characteristic curve analysis and was selected as a possible predictor of @entity1468 . We found a sensitivity of 47% and a specificity of 76% with use of a percentage of activated platelets above 2% as a positive test. Likelihood ratios were 1.94 for positive likelihood and 0.69 for negative likelihood. Univariate logistic regression analysis results were odds ratio 2.8 (95% confidence interval 1.0 to 7.6). Multivariate logistic regression analysis results were odds ratio 2.9 (95% confidence interval 0.92 to 8.9). However, the odds ratio of first antenatal diastolic blood pressure was two to four times higher than the odds ratio of first-trimester @entity5775 expression. The combination of first-trimester @entity5775 and first antenatal diastolic blood pressure increases the positive likelihood ratio from 1.94 to 9.4, with a sensitivity of 41%, a specificity of 96%, and a negative likelihood ratio of 0.62. CONCLUSIONS: Increased first-trimester @entity5775 expression is an independent risk factor for development of @entity1468 . @entity5775 expression might be useful to identify a subgroup of @entity1 with a high risk for development of @entity1468 , especially in combination with first-trimester antenatal diastolic blood pressure. This method of @entity1 selection may enable more efficient intervention studies in @entity1 at risk than do the selection methods used so far.

Can flow cytometric detection of platelet activation early in pregnancy predict the occurrence of XXXX ? A prospective study.

@entity1 :: ('9606', 'Species') :: ['women', 'patients', 'patient'] @entity644 :: ('MESH:D011507', 'Disease') :: ['proteinuria'] @entity101 :: ('MESH:D006973', 'Disease') :: ['Hypertension in Pregnancy criteria'] @entity2279 :: ('5175', 'Gene') :: ['CD31', 'GPIIa'] @entity8729 :: ('6403', 'Gene') :: ['CD62P', 'P-selectin'] @entity1468 :: ('MESH:D011225', 'Disease') :: ['preeclampsia'] @entity27463 :: ('2811', 'Gene') :: ['CD42b'] @entity5775 :: ('967', 'Gene') :: ['CD63']
@entity1468 :: (MESH:D011225,Disease) :: ['preeclampsia']
