OBJECTIVE: To develop and evaluated a rapid multiplex-qPCR to identify fecal carriers of multi-drug resistant extra-intestinal pathogenic @entity526 clonal groups. METHODS: @entity1 presenting for transrectal prostate biopsy (TPB) at the San Diego Veterans Affairs Medical Center underwent rectal culture immediately prior to TPB. Rectal swabs were streaked @entity2177 -supplemented (4 mg/L) MacConkey agar plates, identified, and susceptibility tested. The same swab was sent to the University of Washington for qPCR test (EST200) targeting two major MDR-ExPEC clonal groups - ST131 and ST69 that combined were expected to represent majority of @entity3284 as well as @entity799 resistant @entity526 . We calculate test characteristics including the area under the receiver operative curve (AUC). RESULTS: We enrolled 104 @entity1 from 11/5/2013 to 6/10/2014. @entity3284 -resistant @entity526 were cultured from 19.2% (20/104) of rectal swabs and 26% (27/104) of all swabs were positive for EST200 by PCR. The test characteristics comparing the EST200 to the culture-based detection of @entity3284 -resistance were 75%, 86%, 94%, and 56%, respectively. The AUC was 0.84 for the EST200 to detect @entity3284 resistance prior to TPB. CONCLUSIONS: Compared to the reference standard rectal culture, EST200 was able to detect majority of @entity3284 resistant @entity526 on rectal swabs prior to prostate biopsy.

Rapid Identification of Rectal Multi-Drug Resistant XXXX Prior to Transrectal Prostate Biopsy.

@entity1 :: ('9606', 'Species') :: ['Men', 'men'] @entity799 :: ('MESH:D015662', 'Chemical') :: ['trimethoprim-sulfamethoxazole'] @entity3284 :: ('D024841', 'Chemical') :: ['fluoroquinolone', 'Fluoroquinolone'] @entity2177 :: ('MESH:D002939', 'Chemical') :: ['ciprofloxacin'] @entity526 :: ('562', 'Species') :: ['E. coli']
@entity526 :: (562,Species) :: ['E. coli']
