The balance between immunogenic and tolerogenic activities in @entity1 immune system strongly depends on microflora-induced pro-and anti-inflammatory activities. @entity880 bacteria (LAB) are important components of microflora. The interactions of the different strains of LAB and the cells of immune system are largely unknown. To assess if @entity6475 would have an effect on the cellular responses profile (proliferation, cytokines synthesis) peripheral blood mononuclear cells (PBMC) model system was used. PBMC were induced by three different strains of LAB: @entity9206 , @entity6043 spp. @entity6330 , @entity1335 . Tested strains were mixed together, in combinations with each other (pairs) or alone. Both, the LAB mixture as well as the pairs and the single LAB strains induced low lymphocyte proliferation (about 10% of ConA-induced response). However, the single LAB strains and their combinations were quite different cytokines inducers. First, @entity9206 was much stronger @entity379 inducer than the LAB mixture, being a few times higher IL-12 stimulator than @entity6330 and @entity1335 . Second, @entity6330 and @entity1335 suppressed @entity9206 -induced @entity379 synthesis to the level equal to that induced by the LAB mixture, limiting IL-12 production by about 30% and 70%, respectively. Third, the LAB strains were good @entity688 and @entity310 inducers, irrespectively of their combinations used. We conclude that LAB strains' pro or anti-inflammatory potentials are at least in part dependent on their composition. Low LAB mixture-induced IL-12 and @entity379 production and relatively high @entity688 and @entity310 expression may represent cellular activities normally induced in vivo by a combined action of bacterial antigens. Their presence is important to limit pro-inflammatory reactions (via @entity688 ) and to provide protection against @entity281 (via @entity310 ).

Combined effect of different XXXX bacteria strains on the mode of cytokines pattern expression in @entity1 peripheral blood mononuclear cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity310 :: ('7124', 'Gene') :: ['TNF-alpha'] @entity6475 :: ('MESH:D013180', 'Disease') :: ['LAB strains composition'] @entity688 :: ('3586', 'Gene') :: ['IL-10'] @entity6330 :: ('1585', 'Species') :: ['bulgaricus', 'L. bulgaricus'] @entity379 :: ('3458', 'Gene') :: ['IFN-gamma'] @entity9206 :: ('1579', 'Species') :: ['L.acidophilus', 'Lactobacillus acidophilus', 'L. acidophilus'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['Lactic acid'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infections'] @entity6043 :: ('1584', 'Species') :: ['Lactobacillus delbrueckii'] @entity1335 :: ('1681', 'Species') :: ['B. bifidum', 'Bifidobacterium bifidum']
@entity880 :: (MESH:D019344,Chemical) :: ['lactic acid']
