@entity6494 cell leukemia-derived factor ( @entity14688 ) is a @entity1 homologue of @entity14688 with many biologic functions including @entity2902 induction, growth promotion, @entity1432 -dependent reducing activity, and radical scavenging activity. The regulatory effect of @entity14688 on the cytotoxic activity of @entity310 was examined by using a @entity1 @entity4812 cell line, U937. When U937 cells were preincubated with recombinant @entity14688 ( @entity68195 ) (0.1-100 micrograms/ml) at 37 degrees C for 30 min, @entity310 -dependent @entity137 on U937 cells was markedly inhibited. This inhibitory effect was as high as 95% in the @entity2454 assay ( @entity68195 100 micrograms/ml) and 85% in the 51Cr-releasing assay ( @entity68195 10 micrograms/ml). After pretreatment of U937 cells with @entity379 to augment the sensitivity to @entity310 , an inhibitory effect of @entity68195 was also found. When U937 cells were washed after preincubation with @entity68195 , resistance to @entity310 -dependent @entity137 was still observed, indicating that @entity68195 inhibited the sensitivity of U937 to @entity310 -dependent @entity137 rather than modifying @entity310 molecules. Scatchard analysis of @entity310 receptors on U937 cells using 125I- @entity310 showed that @entity68195 modulated neither the density nor the affinity of the cell membrane significantly. @entity68195 also reduced the @entity137 induced by anti-Fas IgM mAb which shows @entity137 quite similar to @entity310 . @entity68195 (10 micrograms/ml) reduced 90% of the @entity137 by anti-Fas IgM mAb, without a detectable change either in Fas Ag expression (MFI 58.1 vs 53.3) or in the degradation of anti-Fas IgM mAb as determined by flow cytometric analysis. These findings indicated that the @entity68195 -induced resistance to the cytotoxic effect of @entity310 and anti-Fas mAb was not related to the modulation of the @entity310 receptor or Fas Ag.

Protective activity of XXXX cell leukemia-derived factor ( @entity14688 ) against tumor necrosis factor-dependent @entity137 on U937 cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity310 :: ('7124', 'Gene') :: ['TNF'] @entity4812 :: ('MESH:D016403', 'Disease') :: ['histiocytic lymphoma'] @entity68195 :: ('502674(Tax:10116)', 'Gene') :: ['rADF'] @entity6494 :: ('MESH:D015459', 'Disease') :: ['Adult T'] @entity14688 :: ('7295', 'Gene') :: ['thioredoxin', 'ADF'] @entity1432 :: ('CHEBI:29256', 'Chemical') :: ['thiol'] @entity2454 :: ('MESH:C022616', 'Chemical') :: ['3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide'] @entity379 :: ('3458', 'Gene') :: ['IFN-gamma'] @entity2902 :: ('3559', 'Gene') :: ['IL-2R']
@entity6494 :: (MESH:D015459,Disease) :: ['adult T']
