Rapid, and effective diagnosis of @entity3377 is a challenge for clinicians. Even when diagnosed on-time and therapy is initiated, meticulous following-up appointments are crucial to assure the efficacy of the treatment. Due to shortcomings of serological methods, molecular diagnosis, especially real-time PCR, is adopting the main role in laboratory diagnostic approaches. Thus development of efficient procedures and standardization of the tests will have a great impact on precise detection and quantification of bacterial DNA load which is valuable for medical management of @entity3377 @entity1 . We developed a new TaqMan real-time PCR directed to bcsp31, a shared gene of Brucellae. The bcsp31 gene fragment was cloned into pJET1.2. Recombinant pJET1.2-bcsp31 was linearized by HindIII digestion and the product was used for preparation of standard curve. A panel of brucella spp. and non-Brucella pathogens were tested. No other bacterial genome other than that of Brucellae were detected. According to the results, specificity of the method was 100%. In clinical assessment, the positive control group was comprised of thirty-seven @entity1 with microbiologically confirmed @entity3377 and twenty-five healthy individuals served as the negative control group. By the end of the treatment period, there was a significant decrease in the DNA load of the 37 @entity3377 @entity1 which persisted for the 4 weeks of monitoring after treatment, suggesting that our proposed method is an efficient monitoring tool. Serum samples prior to any treatment were collected from the twenty-five serologically suspected @entity1 , and assessed by our method; 72% of these @entity1 tested positive for @entity3377 .

Efficient Diagnosis and Treatment Follow-up of @entity1 Brucellosis by a Novel Quantitative TaqMan Real-Time PCR assay: a XXXX Clinical Survey.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity3377 :: ('MESH:D002006', 'Disease') :: ['brucellosis']
@entity1 :: (9606,Species) :: ['Human']
