BACKGROUND/AIMS: @entity195 ( @entity195 ) is one of the most common @entity303 among aged @entity1 , and is clinically characterized by progressive @entity2414 , @entity146 and @entity73 . So far, this is no cure for @entity195 . A therapeutic effect of transplantation of mesenchymal stem cells (MSCs) into @entity19 model of @entity195 has been reported, but remains to be further improved. @entity18761 is a transcription factor that plays a critical role in neuronal development, whereas the effects of @entity18761 overexpression in transplanted MSCs on @entity195 are unknown. METHODS: MSCs were isolated from @entity19 bone marrow and induced to overexpress antisense of miRNA-937 (as-miR-937) through adeno-associated virus (AAV)-mediated transduction, and purified by flow cytometry based on expression of a GFP co-transgene in the cells. The @entity18761 levels in @entity19 MSCs were examined in miR-937-modified MSCs by RT-qPCR and by Western blot. These miR-937-modified MSCs were then transplanted into an APP/ @entity20933 transgenic @entity195 model in @entity19 . The effects of saline control, MSCs and asmiR-937 MSCs on @entity195 @entity19 were examined by deposition of amyloid-beta peptide aggregates (Ab), social recognition test (SR), Plus-Maze Discriminative Avoidance Task (PM-DAT) and the levels of @entity9140 ( @entity9140 ) in the @entity19 brain. RESULTS: MSCs expressed high levels of @entity18761 transcripts but low levels of @entity18761 protein. Poor protein vs mRNA levels of @entity18761 in MSCs appeared to result from the presence of high levels of miR-937 in MSCs. miR-937 inhibited translation of @entity18761 mRNA through binding to the 3'-UTR of the @entity18761 mRNA in MSCs. Expression of as-miR-937 significantly increased @entity18761 protein levels in MSCs. Transplantation of as-miR-937-expressing MSCs significantly reduced the deposition of Ab, increased the levels of @entity9140 , and significantly improved the appearance in SR and PM-DAT in @entity195 @entity19 . CONCLUSION: Overexpression of as-miR-937 in MSCs may substantially improve the therapeutic effects of MSCs on @entity195 , possibly through augmenting @entity18761 levels in MSCs.

Therapeutic Effects of Transplantation of As-MiR-937-Expressing Mesenchymal Stem Cells in @entity19 Model of XXXX .

@entity1 :: ('9606', 'Species') :: ['people'] @entity9140 :: ('12064(Tax:10090)', 'Gene') :: ['Brain-derived neurotrophic factor', 'BDNF'] @entity20933 :: ('19164(Tax:10090)', 'Gene') :: ['PS1'] @entity146 :: ('MESH:D001523', 'Disease') :: ['behavioral and learning dysfunction'] @entity303 :: ('MESH:D003704', 'Disease') :: ['dementias'] @entity73 :: ('MESH:D003072', 'Disease') :: ['cognitive deficits'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'murine'] @entity18761 :: ('18994(Tax:10090)', 'Gene') :: ['Brn-4'] @entity195 :: ('MESH:D000544', 'Disease') :: ['AD', "Alzheimer's disease"] @entity2414 :: ('MESH:D008569', 'Disease') :: ['memory loss']
@entity195 :: (MESH:D000544,Disease) :: ['Alzheimer's Disease']
