BACKGROUND: Conventional screening for @entity774 ( @entity774 ) using immunoassays generates a large number of false-positive results. A more specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been introduced to minimize unnecessary follow-ups. However, because of limited data on its use in the Korean population, LC-MS/MS has not yet been incorporated into newborn screening programs in this region. The present study aims to develop and validate an LC-MS/MS method for the simultaneous determination of seven @entity230 in dried blood spots (DBS) for @entity774 screening, and to define age-specific reference intervals in the Korean population. METHODS: We developed and validated an LC-MS/MS method to determine the reference intervals of @entity701 , @entity4418 , @entity4419 , @entity68897 , @entity2671 , @entity2819 , and @entity4420 simultaneously in 453 DBS samples. The samples were from Korean subjects stratified by age group (78 full-term neonates, 76 premature neonates, 89 @entity1 , and 100 adults). RESULTS: The accuracy, precision, matrix effects, and extraction recovery were satisfactory for all the @entity230 at three concentrations; values of intra- and inter-day precision coefficients of variance, bias, and recovery were 0.7-7.7%, -1.5-9.8%, and 49.3-97.5%, respectively. The linearity range was 1-100 ng/mL for @entity701 and 0.5-50 ng/mL for other @entity230 (R  >0.99). The reference intervals were in agreement with the previous reports. CONCLUSIONS: This LC-MS/MS method and the reference intervals validated in the Korean population can be successfully applied to analyze seven @entity230 in DBS for the diagnosis of @entity774 .

Dried Blood Spot Testing for Seven XXXX Using Liquid Chromatography-Tandem Mass Spectrometry With Reference Interval Determination in the Korean Population.

@entity1 :: ('9606', 'Species') :: ['children'] @entity2819 :: ('MESH:D003345', 'Chemical') :: ['corticosterone'] @entity4418 :: ('MESH:D019326', 'Chemical') :: ['17-hydroxyprogesterone'] @entity701 :: ('MESH:D006854', 'Chemical') :: ['cortisol'] @entity4419 :: ('MESH:D003350', 'Chemical') :: ['11-deoxycortisol'] @entity230 :: ('MESH:D013256', 'Chemical') :: ['steroids'] @entity68897 :: ('MESH:C003556', 'Chemical') :: ['21-deoxycortisol'] @entity2671 :: ('MESH:D000735', 'Chemical') :: ['androstenedione'] @entity4420 :: ('CHEBI:16973', 'Chemical') :: ['11-deoxycorticosterone'] @entity774 :: ('MESH:D000312', 'Disease') :: ['congenital adrenal hyperplasia', 'CAH']
@entity230 :: (MESH:D013256,Chemical) :: ['Steroids']
