PURPOSE: To determine if genes can be transferred to fibroblasts in the filtering bleb using adenoviral vectors. MATERIALS AND METHODS: Twelve New Zealand albino @entity95 underwent bilateral full-thickness sclerostomies. On postoperative day 1 an adenoviral vector carrying a reporter gene (lacZ) was injected into the @entity735 and saline was injected into the left eye bleb of each @entity95 . Three @entity95 were euthanized on each of the after days (days 3, 7, 14, and 21). The eyes were enucleated and tissue samples from the filtering bleb were processed for beta-galactosidase activity (a marker for lacZ gene expression) and expression of @entity21973 (a fibroblast marker). The median number of cells per high-power field with both beta-galactosidase activity and @entity21973 expression on days 3, 7, 14, and 21 in the right and left eyes were counted to determine adenoviral-mediated gene expression in fibroblasts. RESULTS: In the adenoviral vector-treated eyes, the median number of cells per high-power field with both beta-galactosidase and @entity21973 expression on days 3, 7, 14, and 21 was 83,100, 1, and 0, respectively. No beta-galactosidase activity was noted in the saline-treated eyes. CONCLUSIONS: Adenoviral vectors can transfer genes to fibroblasts in filtering @entity4530 after @entity726 surgery. The peak efficiency of gene transfer to fibroblasts occurred 7 days after @entity726 surgery. These studies show a potential for genetic manipulation of fibroblast activity in filtering @entity4530 after @entity726 surgery.

Adenoviral-mediated gene transfer to the filtering bleb in XXXX .

@entity21973 :: ('100008924(Tax:9986)', 'Gene') :: ['vimentin'] @entity4530 :: ('MESH:D001768', 'Disease') :: ['blebs'] @entity95 :: ('9986', 'Species') :: ['rabbits', 'rabbit'] @entity726 :: ('MESH:D005901', 'Disease') :: ['glaucoma'] @entity735 :: ('MESH:D005124', 'Disease') :: ['right-eye bleb']
@entity95 :: (9986,Species) :: ['rabbits']
