Antibodies were raised to @entity121 ( @entity121 ) enriched fractions obtained from brains of individuals with @entity195 by extraction with ionic detergent followed by @entity969 gradient centrifugation. Electron microscopic examination showed that the fractions were enriched in @entity195 but contained also lipofuscin, amyloid, granular material and membranous elements. Analysis of these fractions with @entity214 -PAGE stained with Coomassie blue showed only a faint band at approximately 60 kDa while most of the material was excluded from the stacking gel. BALB/c @entity19 were injected weekly with 100 or 200 micrograms of these fractions or corresponding fractions from age-matched control brains. The 3 @entity19 injected with Alzheimer brain, but not the 5 @entity19 injected with control brain fractions, produced antibodies that reacted with central and peripheral nervous system axons, @entity3072 in intact tissue as well as with isolated, @entity214 -treated paired helical filaments. In gel strips antibodies from all 3 @entity19 injected with Alzheimer brain fractions reacted with the 200-kDa and 168-kDa but not the 68-kDa neurofilament subunits. The 3 antisera reacted also with some forms of the @entity32328 . Adsorptions with the insoluble fraction from Alzheimer but not from control brains blocked staining of axons and NFT by all 3 antisera. Adsorption with highly purified neurofilament proteins or with a preparation containing the 200-kDa and 168-kDa neurofilament subunits blocked axon and NFT immunostaining only in one antiserum. Adsorptions with microtubule protein, heat-stable microtubule-associated protein, or a preparation of tau did not completely block immunostaining by any of the 3 antisera. These results demonstrate that fractions enriched with @entity195 helical filaments contain insoluble neurofilament, tau and other yet unidentified antigens.

Antibodies to the neuronal cytoskeleton are elicited by Alzheimer XXXX fractions.

@entity19 :: ('10090', 'Species') :: ['mice'] @entity3072 :: ('MESH:C536599', 'Disease') :: ['Alzheimer neurofibrillary tangles'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity32328 :: ('17762(Tax:10090)', 'Gene') :: ['microtubule-associated protein tau'] @entity195 :: ('MESH:D000544', 'Disease') :: ['Alzheimer PHF', 'Alzheimer disease', 'Alzheimer paired'] @entity121 :: ('MESH:D009135', 'Disease') :: ['PHF', 'paired helical filament'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS']
@entity121 :: (MESH:D009135,Disease) :: ['paired helical filament']
