AIMS: The aim of this study was to identify new small molecules that can inhibit @entity6586 biofilms by in  vitro and in  vivo model. METHODS AND RESULTS: We evaluated the effect of a small molecule @entity69932 / @entity10998 conjugate (2-AI/T) on the formation of Strep.  mutans biofilms by culturing in 96-well plates. @entity137 was assessed through cell culture and intragastrically administering to mice. The anti-biofilm and anti-caries effects were investigated in  vivo. The inhibitive mechanism was detected by isobaric tag for relative and absolute quantification (itraq) and RT-QPCR. In vitro and in  vivo study revealed that 2-AI/T significantly inhibited biofilm formation of Strep.  mutans and is more so than inhibiting planktonic cells without @entity137 . The ribosome and @entity3793 metabolism pathways of Strep.  mutans were significantly regulated by this compound. CONCLUSIONS: These results suggest that the 2-AI/T conjugate is a potent inhibitor that can be potentially developed into a new drug to treat and prevent @entity1547 . SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study to use small molecule from marine natural products, to protect from @entity1547 in  vivo. It has potential broad range application in clinical caries prevention, or as a bioactive ingredient for food applications.

A new small molecule inhibits XXXX biofilms in  vitro and in  vivo.

@entity6586 :: ('1309', 'Species') :: ['Streptococcus mutans'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity', 'Toxicity'] @entity3793 :: ('CHEBI:27570', 'Chemical') :: ['histidine'] @entity10998 :: ('CHEBI:38597', 'Chemical') :: ['triazole'] @entity69932 :: ('MESH:C014973', 'Chemical') :: ['2-amino-imidazole'] @entity1547 :: ('MESH:D003731', 'Disease') :: ['dental caries']
@entity6586 :: (1309,Species) :: ['Streptococcus mutans']
