The distributions of neurons displaying immunoreactivity for two @entity460 binding proteins, @entity68983 and 28Kd calbindin, were studied in the thalamus of @entity5434 . Colocalization experiments were carried out to determine the extent to which @entity68983 - and calbindin-like immunoreactivity was found in the same cells and the extent to which either was localized in GABAergic interneurons. Anterograde and retrograde tracing experiments involving the fluorescent tracer, fast blue, were also used to determine that cells expressing the @entity460 binding proteins projected upon the cerebral cortex. In the dorsal thalamus, nuclei are distinguished by different patterns of @entity68983 -like and calbindin-like immunoreactivity. In certain nuclei, for example the lateral dorsal and anterior pulvinar, neurons express immunoreactivity for only one of the @entity460 binding proteins. In others, neurons in different layers, for example the dorsal lateral geniculate nucleus, or in different compartments, for example the intralaminar nuclei, express immunoreactivity for either @entity68983 or calbindin; in other nuclei, for example the ventral group, neurons are mixed and immunoreactivity for @entity68983 and calbindin is commonly colocalized. In the ventral thalamus and epithalamus, similar patterns are observed. Colocalization of @entity68983 - and @entity1246 -immunoreactivity is found in all cells of the reticular nucleus but only in certain cells in selected nuclei of the dorsal thalamus, namely the dorsal lateral geniculate and magnocellular medial geniculate. No calbindin-positive cells are also GABA-positive. Most @entity68983 and/or calbindin positive cells in the dorsal thalamus project to the cerebral cortex, as indicated by the retrograde tracing studies, and many @entity68983 positive fibres entering the cerebral cortex could also be shown to contain fast blue anterogradely transported from a thalamic injection. Most of the major sensory and motor pathways entering the dorsal thalamus express @entity68983 immunoreactivity. The optic tract also expresses calbindin immunoreactivity but most other calbindin positive fibres entering the thalamus ascend in the midbrain tegmentum. The differential distributions of @entity68983 and calbindin implied by these results suggest that thalamic cells belonging to different functional systems and projecting differentially upon the cerebral cortex can be distinguished by differential expression of these or closely related @entity460 binding proteins. This may yield clues to their differential responsivity to afferent driving.

Differential XXXX Binding Protein Immunoreactivity Distinguishes Classes of Relay Neurons in Monkey Thalamic Nuclei.

@entity5434 :: ('9541', 'Species') :: ['M. fascicularis'] @entity68983 :: ('102144381', 'Gene') :: ['parvalbumin'] @entity1246 :: ('MESH:D005680', 'Chemical') :: ['GABA'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium']
@entity460 :: (MESH:D002118,Chemical) :: ['Calcium']
