The presence of @entity11331 dehydrogenase (11beta-HSD; EC1.1.1.146), the enzyme responsible for the interconversion of @entity701 and @entity821 , in granulosa- @entity8665 (GL) cells is associated with a poor outcome in @entity52 ( @entity52 ). We have developed a simple method of assessing the reductase component of 11beta-HSD in these cells which is sufficiently rapid to provide data on the enzyme's activity prior to embryo replacement. Cells were pooled from follicular aspirates and challenged with @entity821 within 2 h of aspiration. @entity701 secretion was then measured by radioimmunoassay. Conversion of @entity821 to @entity701 was linear for up to 3 h and was completely inhibited by @entity13439 , a specific 11beta-HSD inhibitor. Initial velocity rates were determined for eight @entity821 concentrations (range 0.1-8 micromol/l), and the apparent Km calculated (1.6 +/- 0.4 micromol/l). There was no evidence of substrate/product inhibition and conversion of @entity821 to @entity701 was <2% in all experiments. In subsequent work, cells were challenged with @entity821 (6 micromol/l) for 2 h. Cells challenged for 2 h immediately following purification from follicular aspirates produced varying amounts of @entity701 (range 25-150 nmol/pooled follicles from each @entity1 , n = 10 @entity1 ), while basal outputs were <6 nmol/l. Enzyme activity was also examined in cells on a per follicle basis from individual @entity1 and found to vary considerably (e.g. 19, 53 and 36 nmol/l @entity701 /1000 cells, three follicles). Having established the method for assessing 11beta-reductase activity within GL cells, we performed a small prospective study on a series of 20 @entity1 examining the enzyme activity within 110 individual follicles. 11Beta-reductase activity varied greatly from @entity1 to @entity1 and from follicle to follicle ranging from <0.024-0.57 nmol @entity701 /microg DNA but at present low @entity1 numbers preclude a meaningful correlation between enzyme activity and pregnancy rate. In summary, we have developed a simple, rapid (<8 h) assay for detecting the reductase activity of 11beta-HSD in GL cells isolated from pooled or individual follicles. This procedure is sufficiently quick to aid in the choice of embryo for replacement.

A rapid method for the measurement of the oxoreductase activity of @entity11331 dehydrogenase in granulosa-lutein cells from @entity1 undergoing XXXX .

@entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity821 :: ('MESH:D003348', 'Chemical') :: ['cortisone'] @entity13439 :: ('MESH:D006034', 'Chemical') :: ['glycyrrhetinic acid'] @entity701 :: ('MESH:D006854', 'Chemical') :: ['Cortisol', 'cortisol'] @entity52 :: ('MESH:C566179', 'Disease') :: ['in-vitro fertilization', 'IVF'] @entity11331 :: ('CHEBI:35346', 'Chemical') :: ['11beta-hydroxysteroid'] @entity8665 :: ('MESH:D014975', 'Chemical') :: ['lutein']
@entity52 :: (MESH:C566179,Disease) :: ['in-vitro fertilization']
