OBJECTIVE: To revalidate the conversion factor   @entity1577   for the conversion of @entity1861    P210   transcript levels to the international scale   BCR- ABLIS   in chronic @entity2800   CML    which validated before. METHODS: Peking University @entity1 's Hospital   PKUPH   prepared the exchange samples for revalidation of CFs of 15 laboratories which validated nine or eighteen months ago. The fresh @entity1861   P210      +   bone morrow or peripheral blood nucleated cells were diluted with @entity1861    P210      -   cells to achieve different @entity1861 levels, totally 16 sets and 24 samples per set were prepared. @entity26661 reagent was added in each tube. Each laboratory tested @entity1861 transcript levels of one set of samples. Agreement between BCR-ABLIS of each laboratory and PKUPH was assessed by the Bland- Altman method. For laboratories which did not meet the criteria of revalidation, linear regression equation was derived after the samples with maximum @entity1861 deviation were removed until R  >0.98, then new @entity1577 was calculated. RESULTS: 10 laboratories met the revalidation criteria with both bias within   1.4 fold and 95% limits of agreement within   6 folds, and their CFs still could be used for accurately conversion of BCR-ABLIS. New CFs were recalculated as of 1.8-6.3 folds of their previous CFs in 5 laboratories not met the criteria. CONCLUSION: Revalidation of @entity1577 by sample exchange among laboratories was necessary for accurate and continuous application of BCR- ABLIS, which not only tested the validity of @entity1577 acquired before but also calculated new available CFs for those with invalid CFs.

[A multicenter study on the revalidation of validated conversion factor for the conversion of BCRABL   P210   transcript levels to the international scale in chronic XXXX ].

@entity1 :: ('9606', 'Species') :: ['People'] @entity1861 :: ('25', 'Gene') :: ['BCR- ABL', 'BCR-ABL'] @entity2800 :: ('MESH:D007951', 'Disease') :: ['myeloid leukemia'] @entity26661 :: ('MESH:C411644', 'Chemical') :: ['TRIzol'] @entity1577 :: ('MESH:D003550', 'Disease') :: ['CF']
@entity2800 :: (MESH:D007951,Disease) :: ['myeloid leukemia']
