BACKGROUND: Initiation of the coagulation @entity563 protease cascade in @entity1 cells is mediated by tissue factor (TF), which is a cell surface receptor and cofactor for coagulation factor VII (FVII) and its activated form FVII (FVIIa). Increasing evidence suggests that TF is expressed in a wide range of @entity5 cells and plays important roles in @entity5 progression and @entity3 . In this study, we investigated the association between the expression level of TF transcript and histologic features of @entity11 . METHODS: RNA was extracted from normal brain tissues and @entity11 tissues. We developed and validated a real-time quantitative reverse transcription (RT)-PCR assay, based on fluorescent TaqMan methodology, to quantify TF gene expression and @entity16335 dehydrogenase (GAPDH) at the mRNA level in @entity1 @entity11 . RESULTS: The dynamic range of the assay was 10(3)-10(8) copy/microg RNA. The relationship between Ct and log starting concentration was linear (r2 > or = 0.99). The mean expression of TF in healthy brain tissue was 6.2 x 10(3) copy/microg RNA. Overexpression of TF was found in 42 @entity13 samples, mean value is 2.9 x 10(6) copy/microg RNA. CONCLUSIONS: TF mRNA transcript is expressed in @entity11 and the level of expression correlates with histologic grade of @entity5 . This new simple, rapid, semiautomated assay is a major alternative to Northern blot and competitive quantitative PCR for gene alteration analysis in @entity1 @entity5 and may be a powerful tool for large randomized, prospective cooperative group trials and support future TF-based clinical applications.

Quantitative reverse transcription-PCR measurement of tissue factor mRNA in XXXX .

@entity1 :: ('9606', 'Species') :: ['mammalian', 'human'] @entity13 :: ('MESH:C564230', 'Disease') :: ['brain glioma'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer', 'malignancy', 'tumors'] @entity11 :: ('MESH:D005910', 'Disease') :: ['glioma'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity563 :: ('CHEBI:17822', 'Chemical') :: ['serine'] @entity16335 :: ('MESH:D005986', 'Chemical') :: ['glyceraldehyde-3-phosphate']
@entity11 :: (MESH:D005910,Disease) :: ['glioma']
