@entity1313 are a major cause of @entity5 -related morbidity and mortality. Developing new therapeutics for these @entity5 is difficult, as many of these @entity5 are not easily grown in standard culture conditions. Neurosphere cultures under serum-free conditions and orthotopic xenografts have expanded the range of @entity5 that can be maintained. However, many types of @entity1313 remain difficult to propagate or study. This is particularly true for pediatric @entity1313 such as @entity816 and @entity2946 . This protocol describes a system that allows primary @entity1 @entity1313 to be grown in culture. This quantitative assay can be used to investigate the effect of microenvironment on @entity5 growth, and to test new drug therapies. This protocol describes a system where fluorescently labeled @entity1313 cells are grown on an organotypic brain slice from a juvenile @entity19 . The response of @entity5 cells to drug treatments can be studied in this assay, by analyzing changes in the number of cells on the slice over time. In addition, this system can address the nature of the microenvironment that normally fosters growth of @entity1313 . This @entity1313 organotypic slice co-culture assay provides a propitious system for testing new drugs on @entity1 @entity5 cells within a brain microenvironment.

A XXXX /Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1313 :: ('MESH:D001932', 'Disease') :: ['brain tumor', 'Brain tumors', 'brain tumors'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancers', 'cancer', 'tumors'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity816 :: ('MESH:D001254', 'Disease') :: ['pilocytic astrocytomas'] @entity2946 :: ('MESH:D008527', 'Disease') :: ['medulloblastomas']
@entity1313 :: (MESH:D001932,Disease) :: ['Brain Tumor']
