Cells usually lose adhesion and increase proliferation and migration during malignant transformation. Here, we studied how proliferation can affect the other two characteristics, which ultimately lead to invasion and @entity3 . We determined the expression of @entity2393 , as well as adhesion and migration towards laminin-1, fibronectin, collagens type I and type IV presented by LISP-1 @entity14 cells exposed to 2.5% @entity3733 ( @entity3733 ), an agent capable of decreasing proliferation in this poorly differentiated colorectal cell line. Untreated cells (control), as shown by flow cytometry and monoclonal antibodies, expressed alpha 2 (63.8 11.3% positive cells), alpha 3 (93.3 7.0%), alpha 5 (50.4 12.0%) and alpha 6 (34.1 4.9%) integrins but not @entity48759 . Cells adhered well to laminin-1 (73.4 6.0%) and fibronectin (40.0 2.0%) substrates but very little to collagens. By using blocking monoclonal antibodies, we showed that alpha 2, alpha 3 and alpha 6 mediated laminin-1 adhesion, but neither alpha 3 nor alpha 5 contributed to fibronectin adherence. @entity3733 arrested cells at G0/G1 (control: 55.0 2.4% vs @entity3733 : 70.7 2.5%) while simultaneously reducing alpha 5 (24.2 19%) and alpha 6 (14.3 10.8%) expression as well as c-myc mRNA (7-fold), the latter shown by Northern blotting. Although the adhesion rate did not change after exposure to @entity3733 , alpha 3 and alpha 5 played a major role in laminin-1 and fibronectin adhesion, respectively. Migration towards laminin-1, which was clearly increased upon exposure to @entity3733 (control: 6 2 cells vs @entity3733 : 64 6 cells), was blocked by an antibody against alpha 6. We conclude that the effects of @entity3733 on LISP-1 proliferation were accompanied by concurrent changes in the expression and function of integrins, consequently modulating adhesion/migration, and revealing a complex interplay between function/expression and the proliferative state of cells.

Simultaneous changes in the function and expression of XXXX during the growth arrest of poorly differentiated colorectal cells (LISP-1).

@entity48759 :: ('146;3476', 'Gene') :: ['alpha1, alpha 4, alpha v or 4'] @entity3733 :: ('MESH:D004121', 'Chemical') :: ['dimethyl sulfoxide', 'DMSO'] @entity2393 :: ('3688', 'Gene') :: ['beta 1 integrins'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal cancer'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis']
@entity2393 :: (3688,Gene) :: ['beta 1 integrins']
