@entity2657 is routinely measured using the wet-dry method. Volume, however, is the sum total of all cerebral tissues, including water. Therefore, volumetric change following injury may not be adequately quantified using percentage of @entity4 . We thus tested the hypothesis that dried brains can be reconstituted with water and then re-measured to determine the actual volume. @entity510 ( @entity510 ) was induced by endovascular perforation in adult male @entity35 (n   =   30). Animals were euthanized at 24 and 72 h after evaluation of neurobehavior for determination of brain water content. Dried brains were thereafter reconstituted with equal parts of water (lost from @entity2657 ) and centrifuged to remove air bubbles. The total volume was quantified using hydrostatic (underwater) physics principles that 1 ml water (mass)   =   1 cm(3) (volume). The amount of additional water needed to reach a preset level marked on 2-ml test tubes was added to that lost from @entity2657 , and from the brain itself, to determine the final volume. @entity510 significantly increased both brain water and volume while worsening neurological function in affected @entity35 . Volumetric measurements demonstrated significant @entity4 after @entity510 , in addition to the @entity2657 approach. This modification of the "wet-dry" method permits brain volume determination using valuable post hoc dried brain tissue.

Brain Volume Determination in XXXX Using @entity35 .

@entity35 :: ('10116', 'Species') :: ['Sprague-Dawley rats', 'rats'] @entity4 :: ('MESH:D004487', 'Disease') :: ['edema', 'brain swelling'] @entity2657 :: ('MESH:D001929', 'Disease') :: ['Brain edema', 'brain edema'] @entity510 :: ('MESH:D013345', 'Disease') :: ['SAH', 'Subarachnoid hemorrhage']
@entity510 :: (MESH:D013345,Disease) :: ['Subarachnoid Hemorrhage']
