INTRODUCTION: @entity8704 is the commonly employed technique in histopathology laboratories as a part of calcified tissue preparation for the microscopic examination. Chemical agents are most commonly used for routine @entity8704 procedures, though some agents adversely affect the tissue integrity and staining properties. AIM: To compare the efficacy of various decalcifying agents by histological evaluation of hard and soft tissue components of teeth. MATERIALS AND METHODS: The five decalcifying agents namely 10% @entity32428 ( @entity20298 ), 10% Formal @entity32428 ( @entity7034 ), 10 % @entity5189 (FA), 8% @entity68342 (KF) + 8% @entity5189 (FA) and Neutral Ethylenediamenetetracetic acid ( @entity1098 ) were used to decalcify 60 @entity1 permanent teeth. The specimens were subjected to routine processing, sectioning and staining with @entity1710 and @entity1711 . The stained sections were observed under light microscope by three independent observers and grading was done. STATISTICAL ANALYSIS: Paired sample t-test was done for inter-observer variation. One-way-ANOVA and Post-hoc test was applied to compare the effects of different decalcifying agents. RESULTS: Considering preservation and staining characteristics of both hard and soft tissues, superior results were obtained with 10% @entity20298 followed by 10% FNA and @entity1098 which was according to the respective mean values obtained. But statistically significant difference was shown only with 10% FA _ 8% KF + 8% FA. CONCLUSION: @entity20298 was showing the most efficient result as it balances both tissue integrity and time factor suggesting that it can be used as a stable decalcifying agent for routine histopathological diagnosis.

Qualitative histological evaluation of hard and soft tissue components of XXXX permanent teeth using various decalcifying agents - a comparative study.

@entity20298 :: ('CHEBI:25942', 'Chemical') :: ['HNO3'] @entity1 :: ('9606', 'Species') :: ['human'] @entity68342 :: ('CHEBI:63316', 'Chemical') :: ['Potassium formate'] @entity8704 :: ('MESH:D003649', 'Disease') :: ['Decalcification', 'decalcification'] @entity1098 :: ('MESH:D004492', 'Chemical') :: ['EDTA'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity32428 :: ('MESH:D017942', 'Chemical') :: ['Nitric acid', 'nitric acid'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hematoxylin'] @entity7034 :: ('CHEBI:28741', 'Chemical') :: ['FNA'] @entity5189 :: ('MESH:C030544', 'Chemical') :: ['Formic acid']
@entity1 :: (9606,Species) :: ['human']
