PURPOSE: The somatostatin (SS) receptor subtype 2 (sst2) is the principal mediator of the antiproliferative effects of SS and has the highest affinity for the commercially available SS analogues. The purpose of this study was to evaluate sst2 mRNA expression by quantitative reverse transcription-PCR (RT-PCR) in @entity694 and in corresponding normal tissues. EXPERIMENTAL DESIGN: The expression of sst2 mRNA was measured with a quantitative method based on real time RT-PCR with TaqMan assay in 100 @entity694 and in the corresponding normal tissues. In a limited number of @entity1 , these results were compared with those obtained by in situ hybridization (n = 26) and by in vivo imaging with @entity13098 (n = 17). RESULTS: Results obtained by quantitative RT-PCR on sst2 expression in @entity14 were significantly related to those obtained by in situ hybridization and @entity13098 scintigraphy. Sst2 was expressed in all of the @entity5 investigated without any relationship with localization, grading, and stage of disease. Although the paired, unaffected mucosa tends to express a higher abundance of sst2 than the corresponding cancer samples, this difference did not reach a statistical significance. However, in @entity1 with elevated carcinoembryonic antigen levels (>5 ng/ml) there was a significant loss of sst2 mRNA in the @entity5 when compared with its paired normal tissue. CONCLUSIONS: In this study we confirmed, by a quantitative method, that @entity14 does not express higher concentrations of sst2 mRNA than the corresponding unaffected tissue. Conversely, a loss of sst2 was found in @entity1 with elevated preoperative concentrations of carcinoembryonic antigen, an unfavorable prognostic marker for @entity14 .

Quantitative evaluation of somatostatin receptor subtype 2 expression in sporadic XXXX and in the corresponding normal mucosa.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal cancer'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon cancers'] @entity13098 :: ('MESH:C081788', 'Chemical') :: ['(111)In-pentetreotide']
@entity14 :: (MESH:D015179,Disease) :: ['colorectal tumor']
