Microfluidic devices fabricated from polymers exhibit great potential in biological analyses. @entity13768 ( @entity16549 ) has shown promise as a substrate for rapid prototyping of devices. Despite this, disagreement exists in the literature as to the ability of @entity16549 to support electroosmotic (EO) flow and the stability of that flow over time. We demonstrate that in low ionic strength solutions near neutral in pH. oxidized @entity16549 had a four-fold greater EO mobility (mu(eo)) compared to native @entity16549 . The greater mu(eo) was maintained irrespective of whether glass or @entity16549 was used as a support forming one side of the channel. This enhanced mu(eo) was preserved as long as the channels were filled with an aqueous solution. Upon exposure of the channels to air, the mobility decreased by a factor of two with a half-life of 9 h. The EO properties of the air-exposed, oxidized @entity16549 were regenerated by exposure to strong base. High ionic strength, neutral in pH buffers compatible with living eukaryotic cells diminished the EO flow in the oxidized @entity16549 devices to a much greater extent than in the native @entity16549 devices. For analyses utilizing intact and living cells, oxidation of @entity16549 may not be an effective strategy to substantially increase the mu(eo).

Electroosmotic properties of microfluidic channels composed of XXXX .

@entity16549 :: ('CHEBI:61468', 'Chemical') :: ['PDMS'] @entity13768 :: ('MESH:C013830', 'Chemical') :: ['Poly(dimethylsiloxane)']
@entity13768 :: (MESH:C013830,Chemical) :: ['poly(dimethylsiloxane)']
