Using the @entity375 ( @entity375 ) two-hybrid system and a @entity415 ( @entity415 ) KNOX protein, designated @entity64491 , as bait, we have identified seven distinct interacting proteins from a stolon library of @entity415 . All seven cDNAs are members of the BEL1-like family of transcription factors. Among these proteins, there are at least four regions of high sequence conservation including the homeodomain, the @entity1521 - @entity669 - @entity1521 three- @entity535 loop extension, the SKY box, and a 120- @entity535 region upstream from the homeodomain. Through deletion analysis, we identified a protein-binding domain present in the @entity1473 end of the KNOX domain of @entity64491 . The protein-binding domain in the BEL1 protein is located in the @entity1472 -terminal one-half of the 120-residue conserved region of the BELs. RNA-blot analysis showed differential patterns of RNA accumulation for the BELs in various @entity415 organs. The level of @entity28132 mRNA increased in response to a short-day photoperiod in both leaves and stolons. Similar to sense mutants of @entity64491 , transgenic lines that overexpressed @entity28132 exhibited enhanced tuber formation even under noninductive conditions. Unlike @entity64491 sense lines, however, these BEL lines did not exhibit the extreme leaf and stem morphology characteristic of KNOX overexpressers and displayed a more rapid rate of growth than control plants. Both @entity28132 and @entity64491 sense lines exhibited an increase in cytokinin levels in shoot tips. @entity28132 lines also exhibited a decrease in the levels of GA 20-oxidase1 mRNA in stolon tips from long-day plants. Our results demonstrate an interaction between KNOX and BEL1-like transcription factors of @entity415 that may potentially regulate processes of development.

Interacting transcription factors from the three- XXXX loop extension superclass regulate tuber formation.

@entity1472 :: ('CHEBI:46882', 'Chemical') :: ['amino'] @entity28132 :: ('102577460', 'Gene') :: ['StBEL5'] @entity64491 :: ('102577616', 'Gene') :: ['POTH1'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity415 :: ('4113', 'Species') :: ['potato', 'Solanum tuberosum'] @entity1521 :: ('CHEBI:26271', 'Chemical') :: ['proline'] @entity375 :: ('4932', 'Species') :: ['yeast', 'Saccharomyces cerevisiae'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine'] @entity1473 :: ('CHEBI:46883', 'Chemical') :: ['carboxy']
@entity535 :: (CHEBI:33704,Chemical) :: ['amino acid']
