STUDY OBJECTIVES: Inhalation of hypertonic saline or @entity2656 solutions acutely increases mucociliary clearance. Because increased clearance is often coupled with increased mucus secretion, we hypothesized that @entity1602 would stimulate mucus secretion. SETTING AND SUBJECTS: The isolated tracheae of healthy young adult ferrets were studied in a basic research laboratory. MEASUREMENTS AND RESULTS: We demonstrated that there was a dose-dependent increase in @entity3558 secretion by enzyme-linked lectin assay after incubation with 1.69 g/dL (597 mOsm/L), 3.69 g/dL (1,192 mOsm/L), 5.69 g/dL (1,823 mOsm/L), and 10.69 g/dL (3,612 mOsm/L) of saline solution over Krebs-Henseleit solution control (288 mOsm/L) [p < 0.01 for 1.69 g/dL of saline solution and p < 0.0001 for others]. @entity2656 solution, 15 g/dL (1,040 mOsm/L), also significantly increased @entity3558 secretion (n = 4, p < 0.005). There was a 47% and 54% increase in secretion of the serous cell product lysozyme after exposure to 3.69 g/dL (1,192 mOsm/L) and 10.69 g/dL (3,612 mOsm/L) saline solutions, respectively (n = 5, p < 0.05). Secretion was only stimulated when the hyperosmolar exposure was on the luminal side of the epithelium. @entity3558 secretion was induced within minutes of 3.69 g/dL of saline solution exposure, and this increased @entity3558 secretion quickly peaked. The ratio of @entity3558 to lysozyme secretion was approximately 2. This ratio appeared to be independent of the osmotic concentration of the stimulus and therefore of secretory rate. CONCLUSIONS: Mucus secretion is markedly stimulated in response to hyperosmolarity. This may be a protective response. These results also suggest that the therapeutic use of hyperosmolar aerosols should be evaluated with care when used for @entity1 with mucus @entity73 mucus clearance.

XXXX stimulate mucus secretion in the ferret trachea.

@entity2656 :: ('MESH:D008353', 'Chemical') :: ['Mannitol', 'mannitol'] @entity1 :: ('9606', 'Species') :: ['patients'] @entity73 :: ('MESH:D003072', 'Disease') :: ['hypersecretion and impaired'] @entity3558 :: ('100508689', 'Gene') :: ['Mucin', 'mucin'] @entity1602 :: ('MESH:D006944', 'Disease') :: ['hyperosmolar agents']
@entity1602 :: (MESH:D006944,Disease) :: ['Hyperosmolar solutions']
