UNASSIGNED: The effect of the combined use of chitosan, nisin and @entity19794 on the growth of @entity16085 , @entity24816 and psychrophilic bacteria isolated from fish was investigated in broth by means of minimum inhibitory concentrations (MIC). Furthermore, the sites of @entity131 caused by mentioned antimicrobials and their combinations on @entity16085 and @entity24816 were studied. MIC of antimicrobial mixtures were evaluated by Berembaum design and check board method. Antimicrobials' sites of injury were investigated by the evaluation of cell constituents' release, cell surface hydrophobicity and differential scanning calorimetry. Results depended on antimicrobial used; several combinations inhibited the growth of @entity16085 and @entity24816 and all combinations inhibited psychrophilic bacteria. Besides, some mixtures showed synergistic effects. All the mixtures affected ribosomes and DNA of the studied bacteria. Regarding cellular envelope, antimicrobials acted according to the structural characteristics of target microorganisms. Cell damage was higher when antimicrobials were combined, which could explain the observed synergistic effects. This study demonstrates and justifies the synergistic action of chitosan, nisin and @entity19794 on the inhibition of microorganisms related to fish spoilage and remarks the promissory use of the synergic combination of antimicrobials for fish preservation.

Inhibitory effect and cell damage on bacterial flora of fish caused by chitosan, nisin and XXXX .

@entity131 :: ('MESH:D001930', 'Disease') :: ['cell-injury'] @entity24816 :: ('24', 'Species') :: ['S. putrefaciens', 'Shewanella putrefaciens'] @entity19794 :: ('MESH:D019354', 'Chemical') :: ['sodium lactate'] @entity16085 :: ('1642', 'Species') :: ['Listeria innocua', 'L. innocua']
@entity19794 :: (MESH:D019354,Chemical) :: ['sodium lactate']
