The effects of insulin-like growth factors ( @entity2503 and @entity48859 ) on blastocyst expansion, and of basic fibroblast growth ( @entity33326 ) on trophoblast knob formation were studied by in vitro culture of @entity95 blastocysts. Both growth factors are present in the uterine secretions. Embryo culture was carried out in Ham's F10 supplemented with @entity4280 in the presence or absence of @entity1 recombinant growth factors in concentrations ranging from 1 to 100 ng/ml. @entity2503 stimulated expansion of late preimplantation blastocysts to levels found in vivo; after addition of 10 ng/ml, day-6.0 blastocysts increased their diameter within 24 h to 103% of that of day-7.0 blastocysts expanded in vivo (in comparison to 84% without growth factor), and day-7.0 blastocysts expanded to 108% (in comparison to 76% without growth factor). @entity2503 suppressed the synthesis of a pH 6.3/35-kDa protein. Addition of @entity48859 had no effects. @entity33326 effected formation of trophoblast knobs in day-7.0 blastocysts. After addition of 10 ng/ml, the trophoblast knobs appeared within 12 h of culture. The controls without @entity33326 were negative. The striking increase of @entity33326 concentration in the uterine secretion at day 7.0 is perhaps connected with the formation of trophoblast knobs in vivo. FGF receptor-1 was localized in the trophoblast knobs of day-7.5 blastocysts by the use of immunostaining.

Influence of uterine growth factors on blastocyst expansion and trophoblast knob formation in the XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity2503 :: ('100008668(Tax:9986)', 'Gene') :: ['IGF-1'] @entity33326 :: ('100009068(Tax:9986)', 'Gene') :: ['FGF-2'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity4280 :: ('MESH:D011205', 'Chemical') :: ['polyvinylpyrrolidone'] @entity48859 :: ('100328792(Tax:9986)', 'Gene') :: ['IGF-2']
@entity95 :: (9986,Species) :: ['rabbit']
