Monoclonal antibodies which bind to @entity0 have been used to evaluate the detection of @entity3 in axillary lymph nodes. Three monoclonal antibodies (H59, H71, and H72) were reacted with tissue sections of @entity5 and axillary nodes from 24 mastectomy specimens and four specimens from glandular mastectomies for benign disease. All three antibodies had been shown to react with subsets of normal and malignant breast tissue; did not bind erythroid, myeloid, or lymphoid tissue; and recognized antigens in paraffin-embedded tissue. The antibodies recognized cell surface antigens, and H59 and H72 bound to glycoproteins which are either sloughed or secreted. @entity5 and @entity5 in lymph nodes from the same specimen were always bound by the same antibodies. Antibodies detected unrecognized microscopic @entity5 in nodes from one previously node-negative specimen and two specimens with positive nodes. This suggests that monoclonal antibodies may be useful for detecting metastatic @entity0 in nodes which by light microscopy are negative. Moderate binding of H59 and H72 antibodies to sinus histiocytes and perivascular cells was observed in all uninvolved nodes with @entity1373 obtained from benign and malignant specimens. Thus, breast antigens can be identified in hyperplastic nodes in @entity1 with no evidence of @entity0 . The antigens are detected predominately in the lymphoid sinuses and are bound to nonneoplastic cells. Therefore, breast antigens are regularly being processed and presented by normal lymphoid cells within the sinus. The binding of these monoclonal antibodies to axillary lymph nodes does not necessarily indicate the presence of @entity3 . Dense binding to paracortical single cells was observed in @entity5 -containing lymph nodes and in uninvolved nodes obtained from mastectomy specimens with @entity0 . These cells are infrequent, and their number in an uninvolved node correlates with the pathological stage. They represent either binding to isolated lymphoid cells or metastatic @entity5 . Studies are under way to determine the origin of these cells.

Presence of XXXX antigens in uninvolved axillary lymph nodes.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'primary tumors', 'Primary tumors', 'tumors'] @entity1373 :: ('MESH:D006965', 'Disease') :: ['sinus hyperplasia'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastatic disease']
@entity0 :: (MESH:D001943,Disease) :: ['breast cancer']
