UNASSIGNED: We aim to study the smooth muscle cell differentiation capability of @entity1 placental multipotent mesenchymal stromal cells (hPMSCs) and to identify how endothelial cells recruit hPMSCs participating in vessel formation. hPMSCs from term placentas were induced to differentiate into smooth muscle cells in induction conditions and different matrix substrates. We assessed endothelial cells from umbilical veins for PDGF-BB expression and to induce hPMSC @entity3820 and @entity4104 activation. Endothelial cells were co-cultured with hPMSCs for in vitro angiogenesis. Cell differentiation ability was then further assessed by @entity19 placenta transplantation assay. hPMSCs can differentiate into smooth muscle cells; collagen I, IV or laminin supports this differentiation. Endothelial cells expressed significant levels of PDGF-BB and activated @entity4104 transcriptional activity in hPMSCs. Endothelial cell conditioned medium induced hPMSC migration, which was inhibited by @entity4104 siRNA transfection or @entity3820 blocking antibody pretreatment, but not by @entity675 blocking antibody or isotype IgG (p<0.001). hPMSCs can incorporate into endothelial cells with tube formation and promote endothelial cells forming capillary-like networks than endothelial cells alone (tube length: 12024.1    960.1 vs. 9404.2    584.7 pixels; p < 0.001). The capillary-like networks were significantly reduced by hPMSCs pretreated with @entity3820 blocking antibody, but not by @entity675 blocking antibody or isotype IgG (p<0.001). Transplantation of hPMSCs into @entity19 placentas revealed incorporation of the hPMSCs into vessel walls, which expressed alpha-smooth muscle actin, calponin, and smooth muscle myosin (heavy chain) in vivo. In conclusion, endothelial cell-hPMSC interactions occur during vessel development of placenta. Endothelial cell-derived PDGF-BB recruits hPMSCs involved in vascular development via @entity3820 / @entity4104 activation.

XXXX Placenta-Derived Multipotent Stromal Cells Involved in Placental Angiogenesis via the PDGF-BB and @entity4104 Pathways.

@entity1 :: ('9606', 'Species') :: ['human'] @entity675 :: ('5156', 'Gene') :: ['PDGFR-alpha'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity4104 :: ('6774', 'Gene') :: ['STAT3'] @entity3820 :: ('5159', 'Gene') :: ['PDGFR-beta']
@entity1 :: (9606,Species) :: ['Human']
