AIM: : The aim of this study was to analyse the signalling pathways involved in @entity274 vascular responses in @entity101 . METHODS: Vascular function, @entity2404 (TXA2) production, oxidative stress and protein expression were determined in mesenteric resistance arteries (MRAs) from @entity101 (spontaneously @entity101 @entity35 , SHR) and normotensive @entity35 Kyoto (WKY) @entity35 . RESULTS: @entity274 and the TP agonist @entity2872 induced greater contractile responses in MRA from SHR than WKY. Moreover, @entity274 increased @entity3447 production more in SHR than in WKY. The @entity16497 inhibitor @entity35330 reduced @entity274 and @entity2872 -induced contraction and TXA2 release in both strains. The @entity2508 inhibitor @entity4286 reduced @entity274 but not @entity2872 -induced contraction only in SHR arteries. The Rho kinase inhibitor Y26372 reduced @entity274 and @entity2872 -induced contractions only in SHR arteries. Basal @entity16497 , @entity2508 and Rho kinase expression were greater in MRA from SHR than WKY. In SHR, the combination of @entity4286 with the TP antagonist @entity13078 but not with @entity12223 inhibited the @entity274 contraction more than each inhibitor alone. @entity274 and @entity2872 increased @entity1110 oxidase activity and O2 production and decreased mitochondrial membrane potential in vessels from SHR. The effects induced by @entity274 were abolished by inhibitors of TXA2 synthase, @entity2508 and @entity16497 . The mitochondrial antioxidant @entity38342 reduced @entity274 -induced contraction and @entity1110 oxidase activation. CONCLUSION: In arteries from WKY, @entity16497 mediates @entity274 contractile responses by modulating TXA2 release and TXA2 effect. In SHR, @entity274 induces @entity16497 dependent TXA2 release that provokes vascular contractile responses through Rho kinase, @entity16497 and O2 from @entity1110 Oxidase and mitochondria. Moreover, @entity2508 activation contributes to @entity274 contraction in SHR through effects on mitochondria/ @entity1110 Oxidase.

@entity16497 , XXXX and Rho kinase mediate hydrogen peroxide-induced vascular contraction in @entity101 : role of TXA2, @entity1110 oxidase and mitochondria.

@entity38342 :: ('MESH:C555916', 'Chemical') :: ['mitoTEMPO'] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H2O2'] @entity101 :: ('MESH:D006973', 'Disease') :: ['hypertension', 'hypertensive'] @entity2508 :: ('50689;116590', 'Gene') :: ['ERK1/2'] @entity2404 :: ('MESH:D013928', 'Chemical') :: ['thromboxane A2'] @entity3447 :: ('CHEBI:15627', 'Chemical') :: ['TXA2'] @entity4286 :: ('MESH:C093973', 'Chemical') :: ['PD98059'] @entity2872 :: ('MESH:D019796', 'Chemical') :: ['U46619'] @entity13078 :: ('MESH:C045749', 'Chemical') :: ['SQ29548'] @entity12223 :: ('MESH:C108830', 'Chemical') :: ['Y27632'] @entity1110 :: ('MESH:D009249', 'Chemical') :: ['NAD(P)H'] @entity35 :: ('10116', 'Species') :: ['Wistar', 'rats'] @entity35330 :: ('29471(Tax:10116)', 'Gene') :: ['PP1'] @entity16497 :: ('83805(Tax:10116)', 'Gene') :: ['c-Src']
@entity2508 :: (50689;116590,Gene) :: ['ERK1/2']
