BACKGROUND: The authors sought to elucidate the histogenesis of @entity1124 by correlating @entity984 mutation with mucus type in normal epithelium, @entity1373 ( @entity1373 ), and @entity189 . METHODS: Seventy-four @entity189 ( @entity189 ), 23 @entity189 ( @entity189 ), and specimens of 24 normal pancreata were studied. By histochemical staining, normal duct epithelia, areas of @entity1373 , and @entity189 were classified as having sulfo-type or sialo-type mucus. Foci from normal, DC, SC, sulfo-type, or sialo-type specimens were assessed for @entity984 mutation at codon 12 by nested polymerase chain reaction and restriction fragment length polymorphism. RESULTS: Of the @entity189 , 9 were sulfo-type and 65 were sialo-type; all DC specimens were sialo-type, and all normal epithelia were sulfo-type. All foci of sulfo-type, nonneoplastic epithelia were negative for @entity984 mutation. In contrast, 124 of 313 sialo-type @entity1373 foci (40%) had a @entity984 mutation. Of 74 @entity189 , only 3 of 9 sulfo-type @entity5 (33%) were positive for the mutation. Sixty of 65 sialo-type @entity189 (92%) had a @entity984 mutation, whereas 15 of 23 sialo-type @entity189 (65%) had a mutation. @entity984 mutant @entity189 (including both @entity189 and @entity189 ) were associated with @entity984 mutant @entity1373 in 109 of 198 MCHs (55%), whereas @entity189 without a @entity984 mutation had mutations in 6 of 68 MCHs (9%). @entity1373 in normal pancreata revealed @entity984 mutations in 9 of 51 foci (18%). In addition, in @entity984 mutant @entity189 , frequency of @entity984 mutation in @entity1373 increased from 27% (11 of 41 foci) of nonpapillary MCHs to 62% (98 of 157 foci) of papillary MCHs; but in @entity984 wild-type @entity189 , the mutation rate in @entity1373 was unchanged from 12% (3 of 26 foci) to 7% (3 of 42 foci) in nonpapillary and papillary foci, respectively. CONCLUSIONS: These results suggest a strong relationship between the risk of @entity3123 and the presence of combinations of @entity984 gene mutation, papillary growth, and expression of sialomucin in foci of @entity1373 .

Determination of @entity1124 histogenesis by analysis of mucous quality and XXXX mutation.

@entity3123 :: ('MESH:C562463', 'Disease') :: ['pancreatic carcinoma'] @entity1124 :: ('MESH:D021441', 'Disease') :: ['pancreatic ductal carcinoma'] @entity984 :: ('3845', 'Gene') :: ['K-ras'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumors'] @entity1373 :: ('MESH:D006965', 'Disease') :: ['MCH', 'mucous cell hyperplasia'] @entity189 :: ('MESH:D002277', 'Disease') :: ['ductectatic-type carcinomas', 'solid-type carcinomas', 'carcinomas', 'SCs', 'carcinoma', 'DCs']
@entity984 :: (3845,Gene) :: ['K-ras']
