High-fat and high- @entity1735 diets may predispose to @entity1560 , alone or associated with necroinflammation and @entity82 ( @entity1560 ). However, there are few reports about the real effect of these nutrients on hepatocyte redox homeostasis and consequent molecular derangement. Here, we investigated whether different diets would induce oxidative damage in primary @entity35 hepatocytes and thereby affect the activity of phosphatase and tensin homolog ( @entity21202 ). We used @entity35 fed, for 14 weeks, a standard diet (SD), a high-fat/low- @entity1735 diet (HFD-LC), a normal-fat/high- @entity948 diet (NFD-HF), or a high-fat/high- @entity948 diet (HFD-HF). Metabolic and histological parameters were analyzed in blood and liver samples, while oxidative stress markers and related posttranscriptional modification of @entity21202 were analyzed in isolated hepatocytes. Our results indicate that different dietetic hypercaloric regimens caused @entity409 and a significant increase of body and liver weight, as well as elevated plasma levels of @entity493 aminotransferase, @entity1674 and insulin. Hepatocytes from NFD-HF and HFD-HF @entity35 displayed a decrement of cell viability and proliferation rate. Hepatocytes from animals treated with hypercaloric regimens also exhibited oxidative stress greater than SD hepatocytes. Finally, NFD-HF and HFD-HF hepatocytes showed an increased @entity21202 phosphorylation and decreased @entity21202 activity, which seem strongly correlated to an increased glutathionylation of the protein. In conclusion, we demonstrate that @entity948 -enriched diets cause a tissue and hepatocyte damage that might exacerbate those observed in the presence of high-fat alone and might render, via redox homeostasis imbalance, the hepatocytes more prone to posttranslational modifications and activity alteration of @entity21202 .

Redox homeostasis and posttranslational modifications/activity of phosphatase and tensin homolog in hepatocytes from XXXX with diet-induced hepatosteatosis.

@entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis'] @entity1560 :: ('MESH:D005234', 'Disease') :: ['simple steatosis', 'steatohepatitis'] @entity948 :: ('MESH:D005632', 'Chemical') :: ['fructose'] @entity1674 :: ('MESH:D014280', 'Chemical') :: ['triglycerides'] @entity409 :: ('MESH:D008107', 'Disease') :: ['liver damage'] @entity21202 :: ('50557(Tax:10116)', 'Gene') :: ['PTEN'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['carbohydrate'] @entity35 :: ('10116', 'Species') :: ['rat', 'Sprague-Dawley rats', 'rats'] @entity493 :: ('CHEBI:16449', 'Chemical') :: ['alanine']
@entity35 :: (10116,Species) :: ['rats']
