UNASSIGNED: Perivascularly implanted matrix embedded endothelial cells (MEECs) are potent regulators of @entity32 and intimal @entity1373 following @entity1317 . Endothelial cells (ECs) in collagen scaffolds adopt a reparative phenotype with significant therapeutic potential. Although the biology of MEECs is increasingly understood, tuning of scaffold properties to control cell-substrate interactions is less well-studied. It is hypothesized that modulating scaffold degradation would change EC phenotype. Scaffolds with differential degradation are prepared by cross-linking and predegradation. @entity1317 increases degradation and the presence of @entity1358 injury-mediated degradation. MEECs respond to differential scaffold properties with altered viability in vivo, suppressed smooth muscle cell (SMC) proliferation in vitro, and altered @entity68215 expression. When implanted perivascularly to a @entity19 carotid wire injury, tuned scaffolds change MEEC effects on vascular repair and @entity32 . Live animal imaging enables real-time tracking of cell viability, @entity32 , and scaffold degradation, affording an unprecedented understanding of interactions between cells, substrate, and tissue. MEEC-treated injuries improve endothelialization and reduce @entity3960 over 14 d. These data demonstrate the potent role material design plays in tuning MEEC efficacy in vivo, with implications for the design of clinical therapies.

Tuning of Collagen Scaffold Properties Modulates Embedded Endothelial Cell Regulatory Phenotype in Repair of XXXX In Vivo.

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity1373 :: ('MESH:D006965', 'Disease') :: ['hyperplasia'] @entity68215 :: ('16193;17395', 'Gene') :: ['interleukin-6 and matrix metalloproteinase-9'] @entity1358 :: ('MESH:D002292', 'Disease') :: ['MEECs retards'] @entity3960 :: ('MESH:D018235', 'Disease') :: ['SMC hyperplasia'] @entity1317 :: ('MESH:D057772', 'Disease') :: ['Vascular injury', 'vascular injuries']
@entity1317 :: (MESH:D057772,Disease) :: ['Vascular Injuries']
