UNASSIGNED: Persistent @entity281 with high-risk @entity1082 ) types, most often @entity1799 and HPV18, causes all cervical and most @entity5 , and a subset of @entity17539 . Two prophylactic virus-like particle (VLPs)-based vaccines, are available that protect against vaccine type-associated persistent @entity281 and associated disease, yet have no therapeutic effect on existing @entity66 . We have generated recombinant live-attenuated influenza A viruses expressing the @entity1799 oncogenes E6 and E7 as experimental immunotherapeutic vaccine candidates. The @entity11317 life cycle lacks DNA intermediates as important safety feature. Different serotypes were generated to ensure efficient prime and boost immunizations. The immune response to vaccination in C57BL/6 @entity19 was characterized by peptide ELISA and IFN-y ELISpot, demonstrating induction of cell-mediated immunity to @entity1799 E6 and E7 oncoproteins. Prophylactic and therapeutic vaccine efficacy was analyzed in the @entity19 @entity1799 -positive TC-1 @entity5 challenge model. Subcutaneous (s.c.) prime and boost vaccinations of @entity19 with recombinant influenza A serotypes H1N1 and H3N2, followed by challenge with @entity19 cells resulted in complete protection or significantly reduced @entity5 growth as compared to control animals. In a therapeutic setting, s.c. vaccination of @entity19 with established TC-1 @entity5 decelerated @entity5 growth and significantly prolonged survival. Importantly, intralesional vaccine administration induced complete @entity5 regression in 25% of animals, and significantly reduced @entity5 growth in 50% of @entity19 . These results suggest recombinant E6E7 influenza viruses as a promising new approach for the development of a therapeutic vaccine against @entity66 .

Attenuated Recombinant XXXX Expressing @entity1799 E6 and E7 as a Novel Therapeutic Vaccine Approach.

@entity66 :: ('MESH:D004194', 'Disease') :: ['lesions or infections', 'HPV-induced disease'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors', 'anal cancers'] @entity19 :: ('10090', 'Species') :: ['mice', 'murine', 'TC-1'] @entity11317 :: ('11320', 'Species') :: ['influenza A virus'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity1799 :: ('333760', 'Species') :: ['HPV16'] @entity1082 :: ('10566', 'Species') :: ['human papillomavirus (HPV'] @entity17539 :: ('MESH:D010412', 'Disease') :: ['vulvar, vaginal, penile and oropharyngeal carcinomas']
@entity11317 :: (11320,Species) :: ['Influenza A Virus']
