PURPOSE: @entity7995 , @entity32 of the optic nerve, is experienced by most @entity1 with @entity15 ( @entity15 ) and is typically characterized by episodes of acute, monocular @entity1018 . These episodes of @entity32 can lead to damage or degeneration of the retinal ganglion cells (RGCs), the axons of which comprise the optic nerve. @entity6503 ( @entity6503 ) is a well-established model of @entity15 in which @entity19 are immunized to produce a neuroautoimmunity that recapitulates the cardinal hallmarks of @entity1 disease, namely, @entity32 , @entity17 , and @entity75 , spinal cord, and optic nerve. Inflammation-associated oxidative stress plays a key role in promoting @entity602 in @entity6503 . However, the role of oxidative stress in @entity7995 and the associated @entity227 has not been studied. To address this gap in research, we sought to determine how a deficiency in the master antioxidant transcription factor (using @entity10182 [ @entity10182 ]-deficient @entity19 ) affects visual pathology in the @entity6503 model. METHODS: @entity6503 was induced in 8-week-old wild-type (WT) and @entity10182 knockout (KO) @entity19 by immunization against the @entity6504 ( @entity6504 ) peptide antigen. Motor deficits were monitored daily, as was visual acuity using the established functional optokinetic tracking (OKT) assay. @entity19 were euthanized 21 days post-immunization for histological analyses. The optic nerves were paraffin-embedded and stained with @entity1710 and @entity1711 (H_E) or immune cell type-specific antibodies to analyze inflammatory infiltrates. The retinas were flatmounted and stained with an RGC-specific antibody, and the RGCs were counted to assess @entity179 . T-helper (Th) cell-associated cytokines were measured in spleens with enzyme-linked immunosorbent assay (ELISA). Immune analyses of healthy, non- @entity6503 @entity19 were characterized with flow cytometry to assess the baseline immune cell profiles. RESULTS: Female @entity10182 KO @entity19 exhibited more severe @entity6503 -induced @entity51 compared with female WT @entity19 . In both genders, @entity227 , @entity32 of the optic nerve, and @entity528 in KO @entity19 compared with their strain- and age-matched WT counterparts. @entity227 were primarily present in (and only exacerbated in) one eye of each @entity19 . Excess inflammatory cells within the optic nerves of the KO @entity19 were primarily comprised of T-cells, and greater @entity528 in the KO @entity19 was most prevalent in the central retina compared with the peripheral retina. @entity10182 KO spleens exhibited an increased @entity630 - but not Th17-associated immune response. This enhanced pathology in the KO @entity19 was not due to global differences in immune system development between the two genotypes. CONCLUSIONS: This is the first study to report that genetic ablation of @entity10182 exacerbates @entity227 , @entity32 of the optic nerve, and @entity528 in a @entity19 model of @entity15 , suggesting that @entity10182 plays a neuro- and cytoprotective role in @entity6503 -associated @entity7995 .

Loss of @entity10182 exacerbates the @entity227 and XXXX elicited by experimental autoimmune encephalomyelitis.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity1018 :: ('MESH:D054062', 'Disease') :: ['vision loss'] @entity17 :: ('MESH:D003711', 'Disease') :: ['demyelination'] @entity227 :: ('MESH:D014786', 'Disease') :: ['visual deficits', 'EAE elicited more severe visual acuity deficits', 'Visual acuity deficits'] @entity10182 :: ('18024(Tax:10090)', 'Gene') :: ['Nrf2', 'nuclear factor-E2-related factor'] @entity6504 :: ('17441(Tax:10090)', 'Gene') :: ['myelin oligodendrocyte glycoprotein', 'MOG'] @entity630 :: ('57314(Tax:10090)', 'Gene') :: ['Th1'] @entity19 :: ('10090', 'Species') :: ['mice', 'Mice', 'mouse', 'murine'] @entity75 :: ('MESH:D001927', 'Disease') :: ['neurodegeneration of the brain'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity602 :: ('MESH:D013118', 'Disease') :: ['spinal cord damage'] @entity7995 :: ('MESH:D009902', 'Disease') :: ['optic neuritis', 'Optic neuritis'] @entity51 :: ('MESH:D009461', 'Disease') :: ['motor deficits'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hematoxylin'] @entity179 :: ('MESH:D019636', 'Disease') :: ['neurodegeneration'] @entity6503 :: ('MESH:D004681', 'Disease') :: ['Experimental autoimmune encephalomyelitis', 'EAE'] @entity15 :: ('MESH:D009103', 'Disease') :: ['MS', 'multiple sclerosis'] @entity528 :: ('MESH:D009410', 'Disease') :: ['RGC degeneration']
@entity7995 :: (MESH:D009902,Disease) :: ['optic neuritis']
