UNASSIGNED: To allow for a better implementation of external volume expansion to clinical applications for soft tissue regeneration, it is necessary to comprehensively understand the underlying mechanisms. Since @entity1 adipose-derived stem cells (hASCs) play a crucial role in soft tissue enlargement, we investigated the impact of cyclic stretch on gene expression, proliferation rate, and adipogenic differentiation of these cells. After cyclic stretching, RNA was extracted and subjected to DNA microarray analysis and RT-qPCR. Also, the expression of @entity5548 mRNA was analysed by RT-qPCR to test whether mechanical stretch affected adipogenic differentiation of hASCs. Proliferation rate was assessed by alamarBlue assay and Ki-67 staining. Cell cycle analysis was performed with flow cytometry and Western blot. We found that cyclic stretch significantly induced the expression of @entity4188 mRNA. Further, the adipogenic differentiation of hASCs was impaired as was the proliferation. This was partly due to a decrease in @entity1366 and histone H3 phosphorylation, suggesting a @entity851 in the G2 /M phase of the cell cycle. Enrichment analyses demonstrated that stretch-regulated genes were over-represented in pathways and biological processes involved in extracellular matrix organisation, vascular remodelling, and responses to cell stress. Taken together, mechanical stress impaired both the proliferation and adipogenic differentiation, but led to a tissue-remodelling phenotype of hASCs. These data suggest that extracellular matrix remodelling and neoangiogenesis may play a more important role in external volume expansion than proliferation and adipogenesis of hASCs.

The effect of mechanical stress on the proliferation, adipogenic differentiation and gene expression of XXXX adipose-derived stem cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity851 :: ('MESH:D006130', 'Disease') :: ['growth arrest'] @entity4188 :: ('1545', 'Gene') :: ['CYP1B1'] @entity5548 :: ('2167', 'Gene') :: ['FABP4'] @entity1366 :: ('5595;5594', 'Gene') :: ['extracellular signal-regulated kinase (ERK) 1/2']
@entity1 :: (9606,Species) :: ['human']
