BACKGROUND: Biofilms have been shown to be resistant to conventional antibiotic treatment. This study uses a @entity742 biofilm model developed by our department to investigate several novel topical anti-biofilm treatments. METHODS: Staphylococcal biofilms were grown in 54 @entity742 frontal sinuses over 8 days: Each sinus was randomized to (1) no intervention, (2) single @entity23782 flush, (3) regular 12-hourly @entity23782 flushes for 5 days, (4) @entity1792 Zwitterionic Surfactant (CAZS) via hydrodebrider, (5) @entity16891 , (6) CAZS with @entity16891 , (7) CAZS with @entity23782 , and (8) saline regular flushes. @entity742 were @entity50 harvested 1 or 8 days after treatment to assess treatment and any biofilm regrowth. Confocal scanning laser microscopy was used to confirm the presence or absence of biofilms, and the extent of biofilm reduction was quantitated using fluorescent in situ hybridization and colony forming unit counts. RESULTS: In the control @entity742 biofilm coverage averaged 31.7%. Saline and @entity23782 b.d. washes for 5 days had 23% and 0.84% coverage, respectively, when harvested on day 8. A single @entity23782 and @entity2719 wash had 7.7% and 16.2% on day 1 and 5.88% and 16.0% on day 8. CAZS with hydrodebrider had 6.66% on day 1 but 21.95% on day 8 whereas CAZS with hydodebrider and @entity2719 had 13.3% on day 8. CONCLUSION: This study shows that regular treatment with @entity23782 produced the most marked reduction in biofilm surface area coverage (0.84% and 1.25%) with sustained effects over the 8-day follow-up period.

The efficacy of topical antibiofilm agents in a XXXX model of rhinosinusitis.

@entity742 :: ('9940', 'Species') :: ['sheep', 'Sheep'] @entity1792 :: ('MESH:D019343', 'Chemical') :: ['Citric Acid'] @entity16891 :: ('MESH:C027235', 'Chemical') :: ['gallium nitrate'] @entity23782 :: ('MESH:D016712', 'Chemical') :: ['mupirocin'] @entity2719 :: ('MESH:D005708', 'Chemical') :: ['gallium'] @entity50 :: ('MESH:D012851', 'Disease') :: ['sacrificed and the sinus mucosa']
@entity742 :: (9940,Species) :: ['sheep']
