Objective To construct and express a single chain fragment of variety region ( @entity10390 ) against b-site of @entity477 ( @entity477 ), and evaluate the effect of @entity10390 on a- and b-processing of @entity477 . Methods The b-site specific @entity10390 2H10 was amplified and fused with signal sequence Igk and myc tag by PCR to create the expression cassette, which was then subcloned into expression vector pcDNA3.1hyg(+). The plasmid was transferred into CHO cells over-expressing Swedish mutation type @entity1 APP695 (APP695sw/ @entity435 ). The positive clones were identified by @entity14286 . The concentrations of Ab40 and soluble APPa (sAPPa) in the conditioned media supernatant were measured by sandwich ELISA. Results The recombinant eukaryotic expression plasmids were identified by PCR, restriction endonuclease digestion and DNA sequencing. The expression of @entity10390 was confirmed by Western blotting. When @entity10390 2H10 was stably expressed in APP695sw/CHO, the extracellular level of Ab40 decreased by 30.4%, while sAPPa increased by 23.1%. Conclusion The @entity477 b-site @entity10390 2H10 could inhibit the secretion of Ab. Meanwhile, it might be favorable for the a-processing of @entity477 . Hence it can be explored as an inhibitor of @entity6705 ( @entity6705 ).

[Single chain antibody against b-site of XXXX inhibits the generation of b-amyloid peptide].

@entity1 :: ('9606', 'Species') :: ['human'] @entity6705 :: ('23621', 'Gene') :: ['b-site APP-cleaving enzyme', 'BACE'] @entity14286 :: ('MESH:D006921', 'Chemical') :: ['hygromycin B'] @entity477 :: ('351', 'Gene') :: ['APP', 'amyloid precursor protein'] @entity10390 :: ('652070', 'Gene') :: ['scFv'] @entity435 :: ('CHEBI:42485', 'Chemical') :: ['CHO']
@entity477 :: (351,Gene) :: ['amyloid precursor protein']
