Invariant chain (Ii) isoform, through its thyroglobulin-like (Tg) domain, inhibits @entity462 proteases during antigen presentation in vertebrates. In birds, the Ii of @entity22157 (MDIi) has 2 forms: MDIi-1 and @entity1605 (MDIi isoform). To understand the genetic information and expression characteristics of @entity1605 , polymerase chain reaction, and bioinformatic analysis were performed for @entity1605 from healthy adult @entity22157 . The full-length @entity1605 cDNA sequence was found to be 1377-base pairs, encoding a 285-amino acid protein. @entity1605 contains 63 @entity1522 with an insertion sequence in the Tg domain. @entity1605 shares high identity (72.51-94.74%) with the same protein in other birds. The Tg domain of @entity1605 is highly conserved and showed relatively high identity (96.83%) among all tested birds. The molecular structure of the Tg domain supports this conservation. @entity1605 expression was measured in various tissues using real-time quantitative polymerase chain reaction. Similar to MDIi-1, @entity1605 was detected in all tissues but at different levels. Higher expression level was observed in the spleen, intestinal mucosa, and bursa stipe (bursa of Fabricius stipe) than in other tissues. This suggests that @entity1605 , like MDIi-1, plays an essential role in all tissues and that its differential expression may be related to its functions in these tissues. The coexistence of 2 MDIi isoforms indicates that their functions are correlated in @entity22157 . This study improves the understanding of poultry immunology and may be used to improve measures to protect @entity22157 from disease.

Molecular characterization and tissue-specific expression of invariant chain isoform in @entity22157 ( XXXX ).

@entity22157 :: ('8855', 'Species') :: ['Muscovy Duck'] @entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity1605 :: ('OMIM:217000', 'Disease') :: ['MDIi-2'] @entity462 :: ('MESH:D003545', 'Chemical') :: ['cysteine']
@entity22157 :: (8855,Species) :: ['Cairina moschata']
