BACKGROUND: To determine the role of epithelial-mesenchymal transition (EMT) as a potential mechanism contributing to the characteristic @entity8 in @entity1582 , we examined whether @entity1582 light chains (LCs) directly induce EMT in @entity1 renal proximal tubule epithelial cells (PTECs). METHODS: As positive controls we used @entity2533 and @entity205 (CsA), two agents known to induce EMT in PTECs. @entity1 LCs were isolated and purified from the urine of @entity1582 @entity1 with modest @entity177 without evidence of glomerular involvement. @entity1 cells were exposed to kappa LC (25 microM) for periods up to 72 h. RESULTS: LCs induced marked cellular morphological alterations in PTECs, accompanied with increased expression levels of profibrotic @entity2533 , @entity12407 and extracellular matrix components. Using semiquantitative immunoblotting and RT-PCR, we observed that the expression of @entity5962 decreased after 24 h, while the expression of alpha-SMA increased in PTEC after continuous exposure to kappa-LCs. Human serum albumin (HSA; 160 microM) had less potent effect on the expression of EMT-related molecules. Neutralizing @entity2533 antibody blocked CsA-induced EMT but had no effect on LC-exposed cells. LC-induced EMT and the secretions of @entity650 and @entity2842 were, however, markedly attenuated by p38 MAPK interference. The use of @entity12017 or @entity10567 ( @entity10567 ) induced the formation of cell aggregates, and the reacquisition of @entity5962 expression and renal proximal tubule epithelial morphology within the confluent cell monolayer during and after LC exposure. CONCLUSIONS: These findings demonstrate that LC is a direct stimulus for EMT in PTECs. LC-induced EMT involved multiple cytokines, is modulated by p38 MAPK, but appeared independent of the action of @entity2533 . LC-induced EMT may be an important mechanism of @entity8 associated with @entity1582 and may be reversible upon the administration of exogenous @entity10567 .

Myeloma light chains induce epithelial-mesenchymal transition in XXXX renal proximal tubule epithelial cells.

@entity1 :: ('9606', 'Species') :: ['human', 'patients', 'HK-2', 'Human'] @entity650 :: ('3569', 'Gene') :: ['IL-6'] @entity8 :: ('MESH:D007674', 'Disease') :: ['tubulointerstitial renal fibrosis', 'kidney injury'] @entity12407 :: ('51062', 'Gene') :: ['FSP-1'] @entity10567 :: ('116', 'Gene') :: ['PACAP', 'pituitary adenylate cyclase-activating polypeptide'] @entity5962 :: ('999', 'Gene') :: ['E-cadherin'] @entity1582 :: ('MESH:D009101', 'Disease') :: ['multiple myeloma', 'myeloma'] @entity205 :: ('MESH:D016572', 'Chemical') :: ['cyclosporine A'] @entity177 :: ('MESH:D051437', 'Disease') :: ['renal insufficiency'] @entity2533 :: ('7040', 'Gene') :: ['TGF-beta1'] @entity2842 :: ('6347', 'Gene') :: ['MCP-1'] @entity12017 :: ('655', 'Gene') :: ['bone morphogenetic protein-7']
@entity1 :: (9606,Species) :: ['human']
