@entity1723 is associated with a high incidence of @entity301 ( @entity301 ), despite a normal or even increased concentration of high-density lipoprotein (HDL) @entity165 . This paradox may be explained by changes in the antioxidant capacity of HDL, related to paraoxonase ( @entity533 ) activity. HDL compositional changes in subjects with @entity1723 may result in changes in @entity533 activity that are associated with a higher incidence of @entity301 . Single-vertical-spin density-gradient ultracentrifugation was used to isolate seven HDL fractions from serum according to density. @entity533 activity was measured in serum and in the HDL fractions using @entity30670 as substrate. The mean recovery of @entity533 activity in the HDL fractions was 87% (S.D. 12%). @entity301 risk was assessed using B-mode ultrasound to measure carotid artery @entity121 ( @entity121 ). Groups of 35 subjects with @entity1723 [duration of @entity6 18 years (12-32 years) [median (interquartile range)]; glycated haemoglobin 7.67% (1.17%)] and 24 non- @entity6 control subjects were studied. Carotid @entity121 was greater in the @entity6 subjects [0.60 (0.55-0.70) compared with 0.55 (0.45-0.64) mm; P=0.042] and HDL @entity165 concentration was higher [1.53 (0.36) compared with 1.32 (0.34) mmol/l; P=0.031]. There were qualitative differences in HDL in subjects with @entity1723 : HDL particles were @entity1674 -deplete, and there were greater numbers of the larger, more buoyant HDL particles. These properties were not those found to determine @entity533 activity. @entity533 activity increased as HDL particle density increased and particle size decreased; the increase in @entity533 activity was associated with an increase in the ratio of the two HDL surface lipid components, phospholipid and unesterified @entity165 , as particle density increased. @entity533 activity was similar in @entity6 and non- @entity6 subjects [121 (28) and 120 (36) micromol x @entity1009 ) x ml(-1) respectively; P=0.887]. @entity533 activity was not associated with carotid @entity121 in either group. Our results suggest that the @entity533 activities of HDL particles relate to the density, size and composition of the particles. However, @entity533 activity does not appear to contribute to the greater risk of @entity301 in subjects with @entity1723 .

High-density lipoprotein composition and paraoxonase activity in XXXX .

@entity533 :: ('5444', 'Gene') :: ['PON1'] @entity30670 :: ('MESH:C025136|MESH:C570634', 'Chemical') :: ['phenyl acetate'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetic', 'diabetes'] @entity301 :: ('MESH:D003327', 'Disease') :: ['CHD', 'coronary heart disease'] @entity1009 :: ('966', 'Gene') :: ['min(-1'] @entity1674 :: ('MESH:D014280', 'Chemical') :: ['triacylglycerol'] @entity1723 :: ('MESH:D003922', 'Disease') :: ['Type I diabetes'] @entity121 :: ('MESH:D009135', 'Disease') :: ['IMT', 'intima-media thickness'] @entity165 :: ('MESH:D002784', 'Chemical') :: ['cholesterol']
@entity1723 :: (MESH:D003922,Disease) :: ['Type I diabetes']
