BACKGROUND: Current protocols for detection of circulating fibrocytes (CFs) in peripheral blood described in various @entity358 involve complex and time consuming, non standardized techniques. OBJECTIVE: Testing a method to rapidly detect and quantify CFs using whole blood lysis flow cytometry-based assay in @entity1 with @entity2416 ( @entity2416 ) and healthy controls. METHODS: One milliliter of venous blood sample in @entity1098 ( @entity1098 ) from 33 @entity2416 @entity1 and 35 healthy control subjects was collected. Using whole blood lysis method peripheral blood leukocytes were labeled with monoclonal antibodies for cell surface ( @entity2280 and @entity1195 ) and intracellular markers (collagen-1) for flow cytometric analysis. CFs were defined as @entity1195 (+) cells coexpressing collagen-I and @entity2280 molecules. RESULTS: In 29 (87.8%) @entity2416 @entity1 and 10 (28.5%) control subjects, a well-defined highly granular @entity1195 (+) cell population was detected in dot plots generated by side scatter properties of @entity1195 (+) cells. These @entity1195 (+) cells were identified as CFs on the basis of coexpression of collagen-I and @entity2280 ; none of the other cell types in the peripheral blood were labeled with these monoclonal antibodies. In @entity2416 @entity1 the percentage of CFs was significantly higher compared to healthy controls (median (range): 1.37% (0.52-5.65) and 1.04% (0.1-1.84), respectively; P = 0.03). CONCLUSIONS: Whole blood lysis method combined with fluorescence-activated cell sorting (FACS) allows detecting a well-defined homogeneous population of CFs. This method is simple, reproducible, and provides an accurate and rapid estimation of CFs.

Rapid detection of circulating fibrocytes by flowcytometry in XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity1195 :: ('5788', 'Gene') :: ['CD45'] @entity2416 :: ('MESH:D054990', 'Disease') :: ['IPF', 'idiopathic pulmonary fibrosis'] @entity358 :: ('MESH:D008171', 'Disease') :: ['pulmonary and nonpulmonary disorders'] @entity2280 :: ('947', 'Gene') :: ['CD34'] @entity1098 :: ('MESH:D004492', 'Chemical') :: ['EDTA', 'ethylenediaminetetraacetic acid']
@entity2416 :: (MESH:D054990,Disease) :: ['idiopathic pulmonary fibrosis']
