Activated macrophages play an important role in many inflammatory diseases including @entity326 and @entity731 . However, the molecular mechanisms limiting macrophage activation are not completely understood. Members of the tripartite motif (TRIM) family have recently emerged as important players in innate immunity and antivirus. Here, we systematically analyzed mRNA expressions of representative TRIM molecules in @entity1 @entity1357 -derived macrophages activated by different toll-like receptor (TLR) ligands. Twenty-nine TRIM members were highly induced (>3 fold) by one or more TLR ligands, among which 19 of them belong to TRIM C-IV subgroup. Besides @entity6540 , @entity69177 and @entity69178 were shown to be upregulated by @entity6800 and @entity2939 ligands as previous reported, we identified a novel group of TRIM genes ( @entity69179 , 15, 31, 34, 43, 48, 49, 51 and 61) that were significantly up-regulated by @entity6800 and @entity2939 ligands. In contrast, the expression of @entity69180 was down-regulated by @entity6800 and @entity2939 ligands in both @entity1 and @entity19 macrophages. The alternations of the TRIM proteins were confirmed by Western blot. Finally, overexpression of @entity69180 significantly suppressed LPS-induced macrophage activation, whereas siRNA-mediated knockdown of @entity69180 enhanced LPS-induced macrophage activation. Taken together, the study provided an insight into the TLR ligands-induced expressions of TRIM family in macrophages.

Expression profiling of TRIM protein family in XXXX -derived macrophages following TLR stimulation.

@entity1 :: ('9606', 'Species') :: ['human'] @entity6800 :: ('7098', 'Gene') :: ['TLR3'] @entity1357 :: ('2736', 'Gene') :: ['THP1'] @entity69179 :: ('9830', 'Gene') :: ['TRIM14'] @entity6540 :: ('6737', 'Gene') :: ['TRIM21'] @entity326 :: ('MESH:D012772', 'Disease') :: ['septic shock'] @entity2939 :: ('7099', 'Gene') :: ['TLR4'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity69178 :: ('10475', 'Gene') :: ['TRIM38'] @entity731 :: ('MESH:D050197', 'Disease') :: ['atherosclerosis'] @entity69177 :: ('10346', 'Gene') :: ['TRIM22'] @entity69180 :: ('286827', 'Gene') :: ['TRIM59']
@entity1357 :: (2736,Gene) :: ['THP1']
