Our previous studies demonstrated that @entity8197 is cytotoxic to @entity35 platelets. In an attempt to assess the relative contributions of enzymatic redox cycling versus arylation in @entity8197 -induced @entity137 , we have studied three @entity15942 with different mechanisms of action: @entity50785 ( @entity50785 ; pure redox cycler), @entity8197 (both redox cycler and arylator), and @entity3276 (BQ; pure arylator). BQ was more toxic to @entity35 platelets than @entity8197 , while @entity50785 did not cause LDH leakage at all. Cellular uptake kinetics revealed that @entity50785 concentration taken up by the cells was equivalent to that decreased in incubation medium. On the other hand, the concentrations of BQ and @entity8197 taken into the cells were significantly lower than the decreases in concentrations seen in the incubation medium. This suggests indirectly that BQ and @entity8197 may have undergone arylation, binding to @entity2041 ( @entity2197 ) or protein @entity1433 . The difference in arylation capacity between BQ and @entity8197 was well correlated with their relative @entity137 (LDH leakage) observed in platelets. All three @entity15942 caused a rapid, extensive depletion of intracellular @entity2197 in platelets. Treatments with BQ and @entity8197 did not result in formation of @entity9492 ( @entity9492 ), whereas @entity50785 showed a time-dependent increase in @entity9492 . Altogether, these results suggest that enzymatic redox cycling does not play a critical role in @entity3276 -induced @entity137 in @entity35 platelets, while arylation is likely to be @entity3276 's primary mechanism of action.

The relative importance of oxidative stress versus arylation in the mechanism of XXXX -induced @entity137 to platelets.

@entity3276 :: ('MESH:C004532', 'Chemical') :: ['quinone', '1,4-benzoquinone'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity1433 :: ('MESH:D013438', 'Chemical') :: ['thiols'] @entity9492 :: ('MESH:D019803', 'Chemical') :: ['GSSG', 'oxidized glutathione'] @entity8197 :: ('MESH:D024483', 'Chemical') :: ['menadione'] @entity50785 :: ('MESH:C063002', 'Chemical') :: ['2,3-dimethoxy-1,4-naphthoquinone', 'DMNQ'] @entity2041 :: ('MESH:D005978', 'Chemical') :: ['glutathione'] @entity2197 :: ('CHEBI:16856', 'Chemical') :: ['GSH'] @entity15942 :: ('MESH:D011809', 'Chemical') :: ['quinones'] @entity35 :: ('10116', 'Species') :: ['rat']
@entity3276 :: (MESH:C004532,Chemical) :: ['quinone']
