A @entity95 ear @entity63 model was created using a minimally invasive surgical technique. On one ear, three small skin incisions were made on the vascular pedicles about 1 cm from the base of the ear. The central and cranial arteries were ligated and divided along with their accompanying nerves. A circumferential subcutaneous tunnel was made through the incisions to cut subcutaneous tissues, muscles, nerves, and small vessels. The other ear was used as a normal control. Wounds were made on the ventral side of the ear. Twenty-two @entity95 (14 young adults, four aged, and four @entity6 ) were used. In the 26 pairs of wounds in young adult @entity95 , the mean healing time on the @entity63 ear was 20.5+/-3.4 days vs. 14.9+/-1.6 days on the normal ear (mean+/-SD) when normal saline was used as a dressing. Tissue high-energy @entity1391 contents were higher in the normal ear than in the @entity63 ear. The skin temperature on the @entity63 ear was 1-7 degrees C lower than that on the normal ear. Wound-healing times were longer in the aged and @entity6 @entity95 , but no complications occurred in these @entity95 . The model created by minimally invasive procedure results in little skin disruption, a longer @entity63 time, and a higher success rate as compared with many other models. It can be used in normal animals as well as aged animals, and for the first time, was used successfully in @entity6 animals.

Ischemic XXXX ear model created by minimally invasive surgery.

@entity1391 :: ('CHEBI:26020', 'Chemical') :: ['phosphate'] @entity95 :: ('9986', 'Species') :: ['rabbits', 'rabbit'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetic'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemic']
@entity95 :: (9986,Species) :: ['rabbit']
