We evaluated the antioxidative effect of @entity2493 on the oxidized low-density lipoprotein (LDL)-induced impairment of @entity842 (NO) production in @entity1 umbilical artery, which may be the prime cause of @entity409 in @entity1468 . Umbilical artery sections with intact endothelium were obtained from healthy pregnant @entity1 who were delivered between 37 and 40 wk of gestation. The production of NO in the umbilical arteries was stimulated by adding @entity534 followed by incubation for 60 min. NO concentrations were estimated by measuring @entity3542 ions ( @entity6836 using high-performance liquid chromatography. LDL was oxidized by incubation with 5 microM @entity12239 at 37 degrees C for 4 hr, followed by dialysis at 4 degrees C for 24 hr. Prior to the addition of @entity534 , the segments were treated with native or oxidized LDL (0, 50, 100, 200, 400 microg/mL), or were pre-treated with either @entity2656 (50 mM) or @entity2493 (20, 100, 500 microM) before adding oxidized LDL. Changes in @entity534 -induced @entity6836 production were expressed as a percentage of @entity6836 production at the end of pre-incubation. Treatment with oxidized LDL significantly reduced @entity534 -induced @entity6836 production (P<0.05), while @entity6836 production did not change by incubation with native LDL. Pre-treatment with @entity2493 significantly increased @entity6836 production that had been decreased by oxidized LDL (P<0.05). Similarly, pre-treatment with @entity2656 reversed the oxidized LDL-induced reduction in @entity6836 production (P<0.05). These results indicate that @entity2493 protects against oxidized LDL-induced inhibition of NO production in the endothelium of @entity1 umbilical arteries, most likely through its ability to scavenge @entity3787 radicals.

@entity2493 protects against oxidized low-density lipoprotein-induced inhibition of @entity842 production in XXXX umbilical artery.

@entity1 :: ('9606', 'Species') :: ['women', 'human'] @entity2656 :: ('MESH:D008353', 'Chemical') :: ['mannitol'] @entity3542 :: ('CHEBI:16301', 'Chemical') :: ['nitrite'] @entity409 :: ('MESH:D008107', 'Disease') :: ['endothelial dysfunction'] @entity534 :: ('CHEBI:16467', 'Chemical') :: ['L-arginine'] @entity2493 :: ('MESH:D008550', 'Chemical') :: ['melatonin'] @entity3787 :: ('MESH:D017665', 'Chemical') :: ['hydroxyl'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity1468 :: ('MESH:D011225', 'Disease') :: ['pre-eclampsia'] @entity6836 :: ('CHEBI:29785', 'Chemical') :: ['NO(2)', 'NO(2)(-)'] @entity12239 :: ('CHEBI:31440', 'Chemical') :: ['CuSO(4)']
@entity1 :: (9606,Species) :: ['human']
