OBJECTIVES: To determine the @entity4163 composition in @entity1 milk during lactation, and to evaluate the biological significance. METHODS: The gangliosides extracted from 68 milk samples were examined with a high performance thin layer chromatography (HPTLC). RESULTS: The concentration of total lipid bound @entity3167 was 7.8-10.4 microliters/ml. Four @entity7746 were found and designated as GM3, @entity20577 , GX1 and GX2, respectively. GX1 and GX2, which had not been described previously, were tentatively assumed to be gangliosides of the c-pathway because they did not react to the GA1 antibody after sialidase treatment. @entity20577 and GM3 were the major gangliosides in @entity1 milk (accounted for 50%-65%). CM3 decreased sharply at eight days postpartum. Both GM3 and @entity20577 changed significantly during lactation (GM3, r = 0.65, P < 0.01; @entity20577 , r = -0.58, P < 0.01). CONCLUSIONS: Four gangliosides, GM3, @entity20577 , GX1 and GX2 were found in @entity1 milk. Both GM3 and @entity20577 changed significantly during lactation.

[Detection of gangliosides in XXXX milk with a high performance thin layer chromatography (HPTLC)].

@entity1 :: ('9606', 'Species') :: ['human'] @entity7746 :: ('MESH:D005732', 'Chemical') :: ['gangliosides'] @entity3167 :: ('MESH:D019158', 'Chemical') :: ['sialic acid'] @entity20577 :: ('117189', 'Gene') :: ['GD3'] @entity4163 :: ('CHEBI:28892', 'Chemical') :: ['ganglioside']
@entity1 :: (9606,Species) :: ['human']
