UNASSIGNED: The differentiation of urothelial cells results in normal terminally differentiated cells or by alternative pathways in low-grade or high-grade @entity189 . Treatments with traditional surgical and chemotherapeutical approaches are still inadequate and expensive, as @entity150 are generally highly recurrent. In such situations, alternative approaches, using irradiation of the cells and nanoparticles, are promising. The ways in which urothelial cells, at different differentiation levels, respond to UV-irradiation (photolytic treatment) or to the combination of UV-irradiation and nanoparticles (photocatalytic treatment), are unknown. Here we tested @entity137 of UV-irradiation on (i) normal porcine urothelial cells (NPU), (ii) @entity1 low-grade @entity5 cells (RT4), and (iii) @entity1 high-grade @entity5 cells (T24). The results have shown that 1 minute of UV-irradiation is enough to kill 90% of the cells in NPU and RT4 cultures, as determined by the live/dead viability assay. On the other hand, the majority of @entity1 cells survived 1 minute of UV-irradiation. Moreover, even a prolonged UV-irradiation for 30 minutes killed <50% of @entity1 cells. When @entity1 cells were pre-supplemented with mesoporous @entity3759 microbeads and then UV-irradiated, the viability of these high-grade @entity5 cells was reduced to <10%, which points to the highly efficient cytotoxic effects of @entity3759 photocatalysis. Using electron microscopy, we confirmed that the mesoporous @entity3759 microbeads were internalized into @entity1 cells, and that the cell's ultrastructure was heavily compromised after UV-irradiation. In conclusion, our results show major differences in the sensitivity to UV-irradiation among the urothelial cells with respect to cell differentiation. To achieve an increased @entity137 of @entity5 cells, the photocatalytic approach is recommended.

Combined cytotoxic effect of UV-irradiation and XXXX microbeads in normal urothelial cells, low-grade and high-grade @entity5 cells.

@entity1 :: ('9606', 'Species') :: ['human', 'T24'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity5 :: ('MESH:D009369', 'Disease') :: ['urothelial cancer'] @entity189 :: ('MESH:D002277', 'Disease') :: ['urothelial carcinomas'] @entity150 :: ('MESH:D001749', 'Disease') :: ['bladder tumours'] @entity3759 :: ('CHEBI:32234', 'Chemical') :: ['TiO2']
@entity3759 :: (CHEBI:32234,Chemical) :: ['TiO2']
