@entity232 is a worldwide uncontrolled @entity4801 due to the lack of effective drug and vaccine. To speed up effective drug development, we need powerful methods to rapidly assess drug effectiveness against the intracellular form of @entity3667 in high throughput assays. Reporter gene technology has proven to be an excellent tool for drug screening in vitro. The effects of reporter proteins on parasite infectivity should be identified both in vitro and in vivo. In this research, we initially compared the infectivity rate of recombinant @entity3667 expressing stably enhanced green fluorescent protein (EGFP) alone or EGFP-luciferase (EGFP-LUC) with the wild-type strain. Next, we evaluated the sensitivity of these parasites to @entity2379 ( @entity237 ) as a standard drug in 2 parasitic phases, promastigote and amastigote. This comparison was made by @entity98 and @entity842 (NO) assay and by quantifying the specific signals derived from reporter genes like EGFP intensity and luciferase activity. To study the amastigote form, both B10R and @entity1357 macrophage cell lines were infected in the stationary phase and were exposed to @entity237 at different time points. Our results clearly revealed that the 3 parasite lines had similar in vitro infectivity rates with comparable parasite-induced levels of NO following interferon-y/lipopolysaccharide induction. Based on our results we proposed the more reporter gene, the faster and more sensitive evaluation of the drug efficiency.

In Vitro Infectivity Assessment by Drug Susceptibility Comparison of Recombinant XXXX Expressing Enhanced Green Fluorescent Protein or EGFP-Luciferase Fused Genes with Wild-Type Parasite.

@entity1357 :: ('2736', 'Gene') :: ['THP-1'] @entity2379 :: ('MESH:D000666', 'Chemical') :: ['amphotericin B'] @entity232 :: ('MESH:D007896', 'Disease') :: ['Leishmaniasis'] @entity3667 :: ('5664', 'Species') :: ['Leishmania', 'Leishmania major'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity237 :: ('D000666', 'Chemical') :: ['AmB'] @entity4801 :: ('MESH:D010272', 'Disease') :: ['parasitic disease']
@entity3667 :: (5664,Species) :: ['Leishmania major']
