Old world hantaviruses, causing @entity7594 ( @entity7594 ), still present a public health problem in Asia and Eastern Europe. The majority of cases has been recorded in China. The aim of our study was to generate @entity1 recombinant neutralizing antibodies to a hantavirus by phage display technology. To preserve the structural identity of viral protein, the panning procedure was performed on native @entity778 propagated in Vero-E6 cells. In total, five complete human recombinant IgG antibodies were produced in a baculovirus expression system. All of them were able to completely neutralize HTN, and Seoul (SEO) virus in a plaque reduction neutralization test (PRNT). Three of these antibodies could also completely neutralize Dobrava (DOB) virus but not Puumala (PUU) virus. All antibodies bind to @entity16363 G2 protein localized in the virus envelope. The sequence areas within the HTN (76-118)-G2 protein detected by five selected antibodies were mapped using peptide scans. Two partial epitopes, 916-KVMATIDSF-924 and 954-LVTKDIDFD-963, were recognized, which presumably are of paramount importance for docking of the virus to host cell receptors. A consensus motif 916-KVXATIXSF-924 could be identified by mutational analysis. The neutralizing antibodies to the most widely distributed hantaviruses causing @entity7594 might be promising candidates for the development of an agent for prevention and treatment of @entity7594 in @entity1 .

XXXX recombinant neutralizing antibodies against @entity16363 G2 protein.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity778 :: ('MESH:D015658', 'Disease') :: ['Hantaan (HTN) (76-118) virus'] @entity16363 :: ('11599', 'Species') :: ['Hantaan virus'] @entity7594 :: ('MESH:D006480', 'Disease') :: ['hemorrhagic fever with renal syndrome', 'HFRS']
@entity1 :: (9606,Species) :: ['Human']
