Oxidation of low density lipoproteins (LDL) is considered a key event in the pathogenesis of @entity731 . Disturbed generation of coagulatory and anticoagulatory factors by endothelial cells contributes to @entity715 and the progression of @entity731 in coronary arteries. In this study, the effects of native LDL (n-LDL) and oxidized LDL (ox-LDL) on @entity1 coronary endothelial cells were measured. The reaction of coronary endothelial cells to LDL were compared with those of cardiac microvascular endothelial cells grown under comparable conditions. LDL was isolated by ultracentrifugation and @entity920 oxidized. The degree of oxidation was expressed as malondialdehyd ( @entity1865 ) equivalents and was 0.78+/-0.14 nM @entity1865 /mg LDL for native LDL and 13.63+/-1.18 nmol @entity1865 /mg LDL for ox-LDL. Basal secretion of @entity3479 and @entity2289 activity were higher in macrovascular endothelial cells. Incubation of n-LDL in concentrations ranging from 3 to 100 microM/ml LDL-protein did not change @entity3479 -secretion, @entity2289 activity or procoagulant activity in both cell types. Ox- @entity24220 (3 to 100 microM/ml LDL protein) decreased @entity3479 secretion in a concentration dependent manner from 30.9+/-1.7 to 13.7+/-30 ng/ml per 24 h per 10(6) cells (P < 0.01), increased @entity2289 antigen from 2772+/-587 to 4441+/-766 ng/ml per 24 h per 10(6) cells (P < 0.05) as well as @entity2289 activity from 34+/-6 to 55+/-9 AU/ml per 24 h per 10(6) cells (P < 0.05) in macrovascular endothelial cells but had only minor effects on microvascular endothelial cells. Procoagulant activity measured as coagulation time, similarly increased only in macrovascular endothelial cells from 197+/-6 to 76+/-6 s/24 h per 10(6) cells (P < 0.05). The effect on @entity2289 secretion showed a dependency to the degree of oxidation and could be completely blocked by the antioxidant @entity5119 . The @entity1289 ( @entity1289 ), which represents an endothelial enzyme not related to coagulation, remained unchanged during incubation with ox-LDL. Basal @entity1289 activity was higher in microvascular endothelial cells. The higher susceptibility of macrovascular endothelial cells to ox-LDL may partially determine the localization of @entity715 formation and the development of @entity731 in hyperlipidemic @entity1 .

XXXX cardiac microvascular and macrovascular endothelial cells respond differently to oxidatively modified LDL.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity24220 :: ('CHEBI:39026', 'Chemical') :: ['LDL'] @entity2289 :: ('5054', 'Gene') :: ['PAI-1'] @entity1289 :: ('1636', 'Gene') :: ['angiotensin converting enzyme', 'ACE'] @entity1865 :: ('CHEBI:566274', 'Chemical') :: ['MDA'] @entity920 :: ('MESH:D003300', 'Chemical') :: ['copper'] @entity5119 :: ('MESH:D011341', 'Chemical') :: ['probucol'] @entity731 :: ('MESH:D050197', 'Disease') :: ['atherosclerosis', 'atherosclerotic lesions', 'atherosclerotic plaques'] @entity3479 :: ('5327', 'Gene') :: ['t-PA'] @entity715 :: ('MESH:D013927', 'Disease') :: ['thrombus', 'thrombosis']
@entity1 :: (9606,Species) :: ['Human']
