In @entity375 , mating pheromones induce @entity1167 through the activation of two MAP kinases, Fus3 and @entity5677 . Here we report the isolation of three mutants, far4, far5, and far6, that have the novel phenotype of regulating both the activity of Fus3 and @entity5677 and the accumulation of glycogen. A far4 mutation constitutively activates Fus3 and @entity5677 , reduces glycogen, and blocks @entity1167 in the presence of alpha factor. In contrast, far5 and far6 mutations increase glycogen and reduce activation of Fus3 and @entity5677 by @entity5681 . far4, far5, and far6 are recessive and not allelic to @entity46887 , @entity70170 , or 14 genes known to regulate the pheromone response. Non-allelic noncomplementation occurs between far6 and both far4 and far5, suggesting that FAR6 functionally interacts with FAR4 and FAR5. Additional observations suggest that FAR4 has functional overlap with @entity70170 , which we also find to regulate glycogen accumulation. Our results suggest that the activation of the mating MAPK cascade and subsequent @entity1167 is influenced by a signal transduction pathway that regulates glycogen. In support of this possibility, we find that Fus3 is activated to a greater extent in a "wimp" strain with defective protein kinase A. Finally, @entity26060 and @entity21022 have been identified as CEN suppressors of far5, suggesting that the microtubule apparatus may regulate the ability of the pheromone response pathway to promote @entity1167 .

far4, far5, and far6 define three genes required for efficient activation of MAPKs Fus3 and XXXX and accumulation of glycogen.

@entity70170 :: ('855073(Tax:4932)', 'Gene') :: ['FAR3'] @entity26060 :: ('856736(Tax:4932)', 'Gene') :: ['BIM1'] @entity46887 :: ('853283(Tax:4932)', 'Gene') :: ['FAR1'] @entity5677 :: ('852931(Tax:4932)', 'Gene') :: ['Kss1'] @entity1167 :: ('MESH:D006323', 'Disease') :: ['G1 arrest'] @entity21022 :: ('850328(Tax:4932)', 'Gene') :: ['BIK1'] @entity375 :: ('4932', 'Species') :: ['Saccharomyces cerevisiae'] @entity5681 :: ('CHEBI:26013', 'Chemical') :: ['pheromone']
@entity5677 :: (852931(Tax:4932),Gene) :: ['Kss1']
