PURPOSE: We generated a @entity1501 -conjugated @entity7170 nanoparticle (porphysome) suitable for multimodal non-invasive active macrophage tracking @entity583 ( @entity583 ). PROCEDURES: Macrophage uptake of @entity1501 -conjugated porphysomes was selective. @entity1501 -porphysome cardiac macrophage tracking was detected in vivo using radioligand and fluorescent imaging. To track post- @entity583 macrophage mobilization, cardiac fluorescence signal in @entity1501 -porphysome-injected @entity19 was measured for 9  day post- @entity583 . Active macrophage phenotype was assessed using immunohistochemistry. RESULTS: Heart active macrophage presence peaked on day 1, returned to baseline by day 3, and peaked again on day 7 post- @entity583 . Macrophages were distributed throughout the left ventricle at day 1, but aggregated within scar tissue at day 7. Macrophage phenotype was pro-inflammatory ( @entity637 (+)) at day 1, whereas scar-resident macrophages expressed anti-inflammatory markers ( @entity1618 , @entity641 ) at day 7. However, day 7 macrophages outside the scar expressed neither pro- nor anti-inflammatory markers. CONCLUSIONS: We established that @entity1501 -porphysomes are suitable for non-invasive imaging of macrophages and used it to investigate active macrophage behavior in the infarcted heart.

Non-invasive Macrophage Tracking Using Novel Porphysome Nanoparticles in the Post- @entity583 XXXX Heart.

@entity1501 :: ('MESH:D005492', 'Chemical') :: ['Folate', 'folate'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity641 :: ('21803(Tax:10090)', 'Gene') :: ['TGFb'] @entity7170 :: ('CHEBI:8337', 'Chemical') :: ['porphyrin'] @entity1618 :: ('16153(Tax:10090)', 'Gene') :: ['IL-10'] @entity583 :: ('MESH:D009203', 'Disease') :: ['MI', 'post-myocardial infarction'] @entity637 :: ('21926(Tax:10090)', 'Gene') :: ['TNFa']
@entity19 :: (10090,Species) :: ['Murine']
