OBJECTIVE: To detect the immunoglobulin(Ig) and T cell receptor( @entity20432 ) gene rearrangement in bone marrow of @entity1893 ( @entity1893 ) @entity1 by using BIOMED-2 standardized system, and to explore the potential clinical significance of Ig/ @entity20432 gene rearrangement. METHODS: DNA was extracted in bone marrow and Formalin-fixed and Paraffin-embedded(FFPE) samples of @entity1893 @entity1 , the Ig/ @entity20432 gene rearrangements were analyzed by using BIOMED-2 multiple primers system and multiplex PCR assay. RESULTS: Among 235 @entity1893 cases, 71.9% showed @entity20432 gene rearrangement. The positive rate of TCRy and the TCRb were 57.9% and 50.2%. Out of 583 @entity1893 cases, 81.6% showed Ig gene rearrangement. The positive rate of @entity7513 and the @entity1016 were 70.7% and 69.3%. @entity11708 @entity1 showed 84.8% @entity7513 rearrangement and 75.8% @entity1016 rearrangement, as compared with FL(34.0%, 50.9%) and DLBCL(9.2%, 16.1%) @entity1 , the difference was statistically significant (P<0.05). Out of Ig rearrangement positive @entity1893 cases, 65 showed @entity20432 gene rearrangement. None @entity20432 rearrangement positive @entity1893 cases showed Ig gene rearrangement, 25 cases(83.3%) showed Ig gene rearrangement in FFPE samples of 30 DLBCL @entity1 , as compared to Ig rearrangement positive rate of bone marrow, the difference was statistically significant (P<0.001). CONCLUSION: BIOMED-2 standardized Ig/ @entity20432 gene rearrangement system shows assistance for @entity706 diagnosis. The PCR sequencing analysis is much more sensitive and specific and has significance for clinical diagnosis.

[Diagnostic Significance of BIOMED-2 Standardized Gene Rearran-gement System in @entity1 with XXXX ].

@entity1 :: ('9606', 'Species') :: ['patients'] @entity706 :: ('MESH:D008223', 'Disease') :: ['lymphoma'] @entity11708 :: ('OMIM:150800', 'Disease') :: ['MCL'] @entity20432 :: ('C007145', 'Chemical') :: ['TCR'] @entity1016 :: ('50802', 'Gene') :: ['IgK'] @entity1893 :: ('MESH:D008228', 'Disease') :: ["non-Hodgkin's lymphoma", 'NHL', 'T-NHL', 'B-NHL'] @entity7513 :: ('3492', 'Gene') :: ['IgH']
@entity1893 :: (MESH:D008228,Disease) :: ['Non-Hodgkin's Lymphoma']
