A model is described which permits the study of localized and generalized arterial @entity2473 in the intact working perfused @entity95 heart with a perfluorochemical ( @entity37553 ) as perfusate. Coronary arteries were visualized by intraatrial injection of Patent Blue Dye with gated photography. Localized @entity2473 resulted from topical spray of @entity112 (40 mumols) on the epicardial surface overlying an obtuse marginal artery. Before and following topical administration of @entity112 , regional coronary flow was determined using radioisotope-labeled microspheres. Generalized arterial @entity2473 was initiated by intraatrial injection of @entity112 (10 mumols). After topical administration, abtuse marginal artery diameter decreased by 57%; large vessel resistance rose 32 fold; 20% rise of total coronary resistance resulted in a slight reduction of total coronary flow (16%). Heart rate, cardiac output, dP/dtmax and myocardial @entity26 consumption did not change. However, regional coronary flow in the @entity1562 supplied by the affected artery diminished 21% resulting in @entity63 changes in redox pairs. After intraatrial injection of @entity112 , changes were more pronounced. Obtuse marginal artery diameter declined by 88% resulting in 3300-fold rise of large vessel resistance. Total coronary resistance increased 150%, coronary flow and cardiac output diminished (56% and 24%). Both heart rate and dP/dtmax increased (16% and 17%). Generalized @entity3361 after intraatrial @entity112 injection resulted in severe metabolic effects: Myocardial @entity26 consumption (-48%); @entity855 (-29%); @entity1734 (-34%); redox ratios, @entity7685 / @entity12036 and @entity880 / @entity2388 , increased by 449% and 114%, respectively. The findings illustrate that localized and generalized @entity3361 can be produced and quantitated in a working heart model.

A model for the study of XXXX induced changes in cardiac metabolism.

@entity1734 :: ('CHEBI:17287', 'Chemical') :: ['creatine phosphate'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemic'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity12036 :: ('MESH:D004099', 'Chemical') :: ['dihydroxyacetone phosphate'] @entity37553 :: ('MESH:C008807', 'Chemical') :: ['FC-43'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity3361 :: ('MESH:D003329', 'Disease') :: ['coronary spasm'] @entity2473 :: ('MESH:D013035', 'Disease') :: ['spasm'] @entity1562 :: ('MESH:D017682', 'Disease') :: ['myocardium'] @entity7685 :: ('MESH:C029620', 'Chemical') :: ['alpha-glycerophosphate'] @entity112 :: ('MESH:D006632', 'Chemical') :: ['histamine'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity2388 :: ('MESH:D019289', 'Chemical') :: ['pyruvate'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity3361 :: (MESH:D003329,Disease) :: ['coronary spasm']
