OBJECTIVE: To evaluate the effectiveness of the tissue-engineered bone substitute loaded with adenovirus mediated @entity1 @entity1841 gene (Adv-hBMP-2) transfected bone marrow derived mesenchymal stem cells (BMSC) in the repair of @entity79 of large animal. METHODS: The right tibial bone defects (2.6 cm) model of 26 @entity1237 were established and divided into 5 groups: I. Adv-hBMP-2 transfected BMSC/calcined bone (CB) group (n = 9); II. @entity9504 ( @entity9504 ) gene transfected BMSC/CB group (n = 6); III. untransfected BMSC/CB group (n = 6); IV. single CB group (n = 3); VI. untreated group (n = 2). The above tissue-engineered bone substitutes were implanted in the bone defects respectively except group VI. Roentgenography, histomorphometrical analysis and biomechanical measurement were studied at various times. RESULTS: X-ray: at 4 - 8th weeks after implantation, more @entity2721 was found in the bone defects of group I. The complete healing rates of group I, II, III, IV, and V were 5/8, 1/5, 0/5, 0/2, 0/1 respectively at 26th week after implantation. Histomorphometrical analysis showed much more new @entity2721 including cortical bone formed in group I than those of other groups. The compression strength of the implanted bone substitute of group I is significantly higher than those of group II and III. CONCLUSION: The tissue-engineered bone substitute loaded with @entity1 @entity1841 gene transfected BMSC can repair @entity79 of large animal ( @entity1237 ).

[BMP-2 gene modified tissue-engineered bone XXXX in @entity1237 ].

@entity1 :: ('9606', 'Species') :: ['human'] @entity9504 :: ('MESH:D000257', 'Disease') :: ['adenovirus-beta-galactosidase', 'Adv-betagal'] @entity1841 :: ('650', 'Gene') :: ['BMP-2', 'bone morphogenetic protein-2'] @entity79 :: ('MESH:D001847', 'Disease') :: ['diaphyseal segmental bone defect'] @entity2721 :: ('MESH:D018213', 'Disease') :: ['bony callus'] @entity1237 :: ('9925', 'Species') :: ['goat', 'goats']
@entity79 :: (MESH:D001847,Disease) :: ['repairing segmental tibial bone defects']
