G-protein signaling plays important roles in asymmetric cell division. In @entity2199 embryos, homologs of receptor-independent G protein activators, @entity69218 and @entity69219 ( @entity69220 ), function together with Galpha ( @entity54566 and @entity69221 ) to generate asymmetric spindle pole elongation during divisions in the P lineage. Although Galpha is uniformly localized at the cell cortex, the cortical localization of @entity69220 is asymmetric in dividing P cells. In this report, we show that the asymmetry of @entity69220 localization depends on @entity5666 and its downstream intermediate @entity69222 . Furthermore, in addition to its involvement in spindle elongation, Galpha is required for the intrinsically programmed nuclear rotation event that orients the spindle in the one-cell. @entity69222 functions antagonistically to the Galpha/ @entity69220 signaling pathway, providing an explanation for how Galpha-dependent force is regulated asymmetrically by PAR polarity cues during both nuclear rotation and anaphase spindle elongation. In addition, Galpha and @entity69222 are required for spindle orientation during the extrinsically polarized division of EMS cells. In this cell, both @entity69220 and @entity69222 are asymmetrically localized in response to the @entity21001 / @entity21002 signaling pathway. Their localization patterns at the EMS/P2 cell boundary are complementary, suggesting that @entity69222 and Galpha/ @entity69220 signaling function in opposite ways during this cell division as well. These results provide insight into how polarity cues are transmitted into specific spindle positions in both extrinsic and intrinsic pathways of asymmetric cell division.

@entity69222 opposes Galpha/GPR signaling to generate asymmetry for spindle positioning in response to PAR and XXXX / @entity21002 signaling.

@entity69221 :: ('171675(Tax:6239)', 'Gene') :: ['GPA-16'] @entity2199 :: ('6239', 'Species') :: ['C. elegans'] @entity69222 :: ('178210(Tax:6239)', 'Gene') :: ['LET-99'] @entity21002 :: ('171722(Tax:6239)', 'Gene') :: ['SRC-1'] @entity54566 :: ('172505(Tax:6239)', 'Gene') :: ['GOA-1'] @entity5666 :: ('175783(Tax:6239)', 'Gene') :: ['PAR-3'] @entity69219 :: ('176318(Tax:6239)', 'Gene') :: ['GPR-2'] @entity21001 :: ('181362(Tax:6239)', 'Gene') :: ['MES-1'] @entity69220 :: ('176318;176210', 'Gene') :: ['GPR-1/2'] @entity69218 :: ('176210(Tax:6239)', 'Gene') :: ['GPR-1']
@entity21001 :: (181362(Tax:6239),Gene) :: ['MES-1']
