Sequence tagged site (STS) markers for eight resistance genes against Puccinia recondita @entity10327 . tritici were used to screen a set of near-isogenic lines of @entity3505 cv. Thatcher containing in total 40 different Lr genes and their alleles. Polymerase chain reaction (PCR) analysis was carried out by using STS, SCAR and CAPS primers specific for the leaf rust resistance genes @entity13716 , Lr9, Lr10, Lr19, Lr24, Lr28, Lr37 and Lr47. The STS, CAPS and SCAR markers linked to resistance genes Lr9, Lr10, Lr19, Lr24, Lr37 and Lr47 were found to be reliable in diverse genetic backgrounds. The amplification product of the @entity13716 gene marker was detected in the susceptible cv. Thatcher and in all of the near-isogenic lines examined except Lr2a, Lr2b, Lr2c and Lr19. The sequence analysis of PCR products amplified in lines @entity13716 , Lr10, Lr28 and in cv. Thatcher indicated that the near-isogenic lines and cv. Thatcher contained in the targeted chromosome region an allele that differed from the original alleles corresponding to @entity13716 *Thatcher (TLR621) and susceptible Thatcher (TH621). The amplification product specific to the STS marker of the @entity13716 gene was amplified in almost all Thatcher near-isogenic lines and in cv. Thatcher because their alleles possessed primer sequences identical to the original allele TLR621. The marker for the Lr28 resistance gene was identified in line Lr28, carrying gene Lr28, and in 21 other near-isogenic lines. The sequencing of PCR products specific to Lr28 and generated in lines @entity13716 , Lr10 and Lr28 indicated that the lines @entity13716 , Lr10 and Lr28 are heterozygous in this region.

Application of STS markers for leaf rust resistance genes in near-isogenic lines of spring XXXX cv. Thatcher.

@entity3505 :: ('4565', 'Species') :: ['wheat'] @entity10327 :: ('239', 'Species') :: ['f. sp'] @entity13716 :: ('100125344(Tax:4565)', 'Gene') :: ['Lr1', 'Lr1/6']
@entity3505 :: (4565,Species) :: ['wheat']
