Myeloid-derived suppressor cells (MDSCs) have an important role in controlling @entity32 . As such, they are both a therapeutic target and, based on the administration of ex vivo-generated MDSCs, a therapeutic tool. However, there are relatively few reports describing methods to generate @entity1 MDSCs, and most of them rely on cells obtained from peripheral blood monocytes. We investigated alternative approaches to the generation of MDSCs from hematopoietic progenitors and monocytes. Purified @entity2280 (+) hematopoietic progenitors from apheresis products and @entity5750 (+) cells isolated from buffy coats were cultured in the presence of different combinations of cytokines. The resulting myeloid cell populations were then characterized phenotypically and functionally. Progenitor cells cultured in the presence of @entity6826 +TPO+ @entity11601 + @entity2841 + @entity650 gave rise to both monocytic (M)- and granulocytic (G)-MDSCs but production of the latter was partially inhibited by @entity2772 . M-MDSCs but not G-MDSCs were obtained by culturing peripheral blood monocytes with @entity2841 + @entity650 or @entity2841 + @entity2533 for 6 days. @entity5750 expression was downregulated in the cultured cells. @entity4634 expression at baseline was lower in hematopoietic progenitor cell-derived than in monocyte-derived MDSCs, but was markedly increased in response to stimulation with LPS+IFN-y. The functionality of the two MDSC subtypes was confirmed in studies of the suppression of allogeneic and mitogen-induced proliferation and by cytokine profiling. Here we describe both the culture conditions that allow the generation of MDSCs and the phenotypical and functional characterization of these cell populations.Immunology and Cell Biology advance online publication, 28 February 2017; doi:10.1038/icb.2017.4.

Myeloid-derived suppressor cells can be efficiently generated from XXXX hematopoietic progenitors and peripheral blood monocytes.

@entity1 :: ('9606', 'Species') :: ['human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity650 :: ('3569', 'Gene') :: ['IL-6'] @entity4634 :: ('29126', 'Gene') :: ['PD-L1'] @entity5750 :: ('929', 'Gene') :: ['CD14'] @entity11601 :: ('2323', 'Gene') :: ['FLT3-L'] @entity2533 :: ('7040', 'Gene') :: ['TGF-b1'] @entity2280 :: ('947', 'Gene') :: ['CD34'] @entity2772 :: ('3562', 'Gene') :: ['IL-3'] @entity6826 :: ('4254', 'Gene') :: ['SCF'] @entity2841 :: ('1437', 'Gene') :: ['GM-CSF']
@entity1 :: (9606,Species) :: ['human']
