@entity69484 ( @entity25910 ) is a type I membrane sialoprotein of the CD34 family, originally described in the epithelial glomerular cells of the kidney (podocytes) in which it plays an important function. @entity25910 can also be found in megakaryocytes and platelets among other extrarenal places. The surface exposure of @entity25910 upon platelet activation suggested it could play some physiological role. To elucidate the function of @entity25910 in platelets, we generated @entity19 with restricted ablation of the @entity25910 gene in megakaryocytes using the Cre-LoxP gene targeting methodology. @entity19 with @entity25910 -null megakaryocytes did not show any apparent phenotypical change and their rates of growth, life span and fertility did not differ from the floxed controls. However, @entity25910 -null @entity19 showed prolonged bleeding time and decreased @entity1423 in response to physiological agonists. The number, size-distribution and polyploidy of @entity25910 -null megakaryocytes were similar to the floxed controls. @entity25910 -null platelets showed normal content of surface receptors and normal activation by agonists. However, the @entity19 bearing @entity25910 -null platelets showed a significant retardation in the @entity30059 -induced occlusion of the carotid artery. Moreover, acute @entity715 induced by the i.v. injection of sublethal doses of collagen and @entity2647 produced a smaller fall in the number of circulating platelets in @entity25910 -null @entity19 than in control @entity19 . In addition, perfusion of uncoagulated blood from @entity25910 -null @entity19 in parallel flow chamber showed reduced adhesion of platelets and formation of aggregates under high shear stress. It is concluded that platelet @entity25910 is involved in the control of hemostasis acting as a platelet co-stimulator, likely due to its pro-adhesive properties.

Diminished thrombogenic responses by deletion of the Podocalyxin Gene in XXXX megakaryocytes.

@entity30059 :: ('MESH:C024555', 'Chemical') :: ['ferric chloride'] @entity19 :: ('10090', 'Species') :: ['mice', 'Mice'] @entity1423 :: ('MESH:D001791', 'Disease') :: ['platelet aggregation'] @entity25910 :: ('27205(Tax:10090)', 'Gene') :: ['podxl', 'Podxl'] @entity2647 :: ('MESH:D010656', 'Chemical') :: ['phenylephrine'] @entity69484 :: ('MESH:C001643', 'Chemical') :: ['Podocalyxin'] @entity715 :: ('MESH:D013927', 'Disease') :: ['thrombosis']
@entity19 :: (10090,Species) :: ['mouse']
