Myostatin decreases muscle mass and this is accomplished, in part, by inhibiting muscle satellite cell proliferation and differentiation by regulating the expression of cell cycle-related proteins (e.g. @entity3544 and @entity15308 ) and myogenic regulatory factors (e.g. myogenin and @entity9171 ). The purpose of this investigation was to determine whether protein ingestion before and after a resistance exercise (RE) bout affects myostatin and cell cycle-related gene expression. Strength-trained middle-aged to older @entity1 were divided into a protein group (61.4 +/- 4.3 years, n = 9) or a placebo group (62.1 +/- 4.2 years, n = 9). Muscle biopsies from the vastus lateralis muscle were taken at rest and 1 and 48 h after a 5 x 10 repetition leg press RE bout. Protein (15 g whey) or non-caloric placebo was taken immediately before and after the RE bout. mRNA expression levels of @entity12560 and related genes (AcvrIIb, @entity61574 , @entity3544 , @entity993 , @entity15308 , myogenin and @entity9171 ) were determined by Taqman probe-based real-time RT-PCR and normalized to @entity7501 mRNA. Myostatin mRNA decreased after a RE bout, but only in the placebo group (P < or = 0.05). Conversely, myostatin-binding protein @entity61574 and cell-cycle kinase @entity15308 mRNA increased only in the protein group (P < or = 0.05). @entity3544 mRNA was increased at 1 h post-RE in placebo (P < or = 0.05) and tended to be increased in the protein group (P = 0.08). @entity12560 , its binding protein and cell cycle-related gene expressions are affected by single RE bout and these responses are further modified by whey protein intake. Therefore, controlling nutrition intake is important when studying gene expression responses to exercise.

The effects of whey protein on myostatin and cell cycle-related gene expression responses to a single heavy resistance exercise bout in trained older XXXX .

@entity1 :: ('9606', 'Species') :: ['men'] @entity9171 :: ('4654', 'Gene') :: ['MyoD'] @entity7501 :: ('2597', 'Gene') :: ['GAPDH'] @entity993 :: ('10671', 'Gene') :: ['p27'] @entity3544 :: ('644914', 'Gene') :: ['p21'] @entity15308 :: ('1017', 'Gene') :: ['cdk2'] @entity12560 :: ('MESH:D055435', 'Chemical') :: ['Myostatin', 'myostatin'] @entity61574 :: ('10272', 'Gene') :: ['FLRG']
@entity1 :: (9606,Species) :: ['men']
