A factor that stimulates @entity2 cells on biological substrata was purified from the @entity1 @entity2220 cell line WART. A partially purified autocrine motility factor-like substance, termed @entity69724 , was added to the lower compartment of Boyden chambers and the filters were coated on the upper, lower or both sides with different concentrations of the extracellular matrix (ECM) components fibronectin, laminin or collagen type IV. These adhesive proteins coated on the lower surface of the filter promoted the migration (haptotaxis) of @entity2 cells. This effect was greatly enhanced by the addition of @entity69724 . In contrast, ECM components (including gelatin) coated on the upper surface or on both filter surfaces did not stimulate @entity5 cell migration. However, the addition of @entity69724 also timulated cell migration under these conditions. @entity69724 did not stimulate migration on filters coated with bovine serum albumin or on uncoated filters. @entity69724 could not be absorbed by fibronectin, laminin, collagen type IV or gelatin, and soluble ECM components did not affect the locomotor effect of @entity69724 . Substrata coated with fibronectin, laminin and collagen type IV induced adhesion and spreading of @entity2 cells in a dose dependent fashion. @entity69724 potentiated adhesion and spreading of @entity5 cells on these substrata but did not in itself mediate adhesion and spreading of the cells. Anti- @entity12974 antibodies inhibited migration to @entity69724 on gelatin and laminin coated filters but did not affect @entity69724 -induced migration on fibronectin or collagen type IV substrata. Anti- @entity16445 monoclonal antibodies inhibited @entity69724 -induced migration on fibronectin coated filters but not such migration on filters coated with other ECM molecules showing that @entity5 cells must interact specifically with ECM components in order to migrate to @entity69724 .

A XXXX derived motility factor that stimulates cell migration on extracellular matrix.

@entity1 :: ('9606', 'Species') :: ['human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity2 :: ('MESH:D008175', 'Disease') :: ['migration of lung carcinoma', 'lung carcinoma'] @entity69724 :: ('MESH:C113529', 'Chemical') :: ['Haptotaxin', 'haptotaxin'] @entity2220 :: ('MESH:C538231', 'Disease') :: ['lung adenocarcinoma'] @entity12974 :: ('3673', 'Gene') :: ['VLA 2'] @entity16445 :: ('3678', 'Gene') :: ['VLA-5']
@entity5 :: (MESH:D009369,Disease) :: ['tumor']
