Two-dimensional @entity2975 gel electrophoresis (2-DE) profiles of @entity1 @entity2 tissue and paired surrounding normal bronchial epithelial tissue were compared. Selected differential protein-spots were identified with peptide mass fingerprinting based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching. Well-resolved and reproducible 2-DE patterns of both the @entity5 and the normal tissues were acquired. The average deviations of spot position were 0.873+/-0.125mm in IEF direction and 1.025+/-0.213mm in SDS-PAGE direction, respectively. For the @entity5 tissues, a total of 1349+/-67 spots were detected and 1235+/-48 spots were matched with an average matching rate of 91.5%. For the corresponding normal tissues, a total of 1297+/-73 spots were detected and 1183+/-56 spots were matched with an average matching rate of 91.2%. A total of 1069+/-45 spots were matched between the @entity5 and the normal tissues. Forty differential proteins between @entity5 and normal tissues were characterized. Some proteins were the products of oncogenes and others were involved in the regulation of cell cycle and signal transduction. These data are valuable for mass identification of differentially expressed proteins involved in lung carcinogenesis, establishing @entity1 @entity2 proteome database and screening molecular marker to further study @entity1 @entity2 .

Comparative proteomics analysis of @entity1 XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung cancer', 'lung squamous carcinoma'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor']
@entity2 :: (MESH:D008175,Disease) :: ['lung squamous carcinoma']
