AIMS: To investigate the relationships between water temperature, bacterial growth, virulence and antigen expression in @entity12177 , the causal agent of cold water vibriosis in @entity12178 ( @entity12178 L.). METHODS AND RESULTS: The significance of sea temperature was investigated using historical clinical and oceanographic data. An upper threshold for disease of approx. 10 degrees C was established. The effects of culture temperature and media type on bacterial growth were studied on solid and in liquid media. The highest rates of cell division were identified at 15 degrees C on solid media and 10 degrees C in liquid media. Outer membrane protein (OMP) expression and serological response in @entity12178 were studied using @entity214 - @entity2975 gel electrophoresis, Western blotting and enzyme-linked immunosorbent assay. A novel 76-kDa OMP produced in unshaken cultures at 10 degrees C was not found to stimulate a specific humoral response. CONCLUSIONS: Diagnostic agar plate-based incubation of suspected @entity12177 should be carried out at 15 degrees C. High yield broth cultures for vaccine production should be incubated at 10 degrees C or lower. SIGNIFICANCE AND IMPACT OF THE STUDY: This study is, to the best of our knowledge, the first to identify different optimal temperatures in a bacterial species cultured on physically different types of media. The evidence presented suggests that @entity12177 and possibly other bacteria destined for vaccine use in poikilothermic organisms should be cultured at temperatures consistent with that at which disease occurs.

Relevance of incubation temperature for XXXX vaccine production.

@entity12178 :: ('8030', 'Species') :: ['Atlantic salmon', 'Salmo salar'] @entity12177 :: ('40269', 'Species') :: ['Vibrio salmonicida', 'V. salmonicida'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['sodium dodecyl sulphate'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide']
@entity12177 :: (40269,Species) :: ['Vibrio salmonicida']
