OBJECTIVE: To observe the effects of different doses of @entity1 parathyroid hormone fragment (hPTH)1-34 on SaoS cells and to explore the signal pathway and mechanism. METHODS: 5 x 10(4) cells/ml SaoS cells were seeded. Doses of 5, 50, 500 and 5 000 micro g/L hPTH1-34 were supplemented respectively. Total RNA was extracted by Trizol kit. @entity623 ( @entity623 ), osteocalcin ( @entity12502 ) and @entity268 concentrations were measured by chemical, radioimmunoassay and competitive protein binding methods. @entity1639 gene expression level was semi-quantified by reverse transcriptase (RT)-PCR. RESULTS: @entity623 activity was higher in 500 micro g/L dose (P < 0.05 vs. control). @entity12502 level was inhibited in 5 000 micro g/L dose (P < 0.05 vs. control and pretreatment). 50 and 500 micro g/L hPTH1-34 enhanced @entity268 level significantly (P < 0.05 vs. control). No obvious increase of @entity268 level was found in 5 000 micro g/L and 5 micro g/L dose groups (P > 0.05 vs. control and pretreatment). @entity1639 expression was higher in 50 and 500 micro g/L group. CONCLUSION: Different doses of hPTH1-34 exert distinct effects on osteoblasts. hPTH1-34 affects @entity623 and @entity1639 depending on protein kinase A signal pathway. hPTH1-34 exerts its action via regulation of osteoblasts by @entity1639 .

[The effects of continuous stimulation with different doses of parathyroid hormone 1-34 fragment on XXXX SaoS-2 cells].

@entity1 :: ('9606', 'Species') :: ['human'] @entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity12502 :: ('632', 'Gene') :: ['BGP'] @entity1639 :: ('2353', 'Gene') :: ['c-fos'] @entity623 :: ('250', 'Gene') :: ['ALP', 'Alkaline phosphatase']
@entity1 :: (9606,Species) :: ['human']
