In order to sustain lifelong production of gametes, many animals have evolved a stem cell-based gametogenic program. In the @entity238 ovary, germline stem cells (GSCs) arise from a pool of primordial germ cells (PGCs) that remain undifferentiated even after gametogenesis has initiated. The decision of PGCs to differentiate or remain undifferentiated is regulated by somatic stromal cells: specifically, @entity11735 ( @entity11735 ) signaling activated in the stromal cells determines the fraction of germ cells that remain undifferentiated by shaping a Decapentaplegic (Dpp) gradient that represses PGC differentiation. However, little is known about the contribution of germ cells to this process. Here we show that a novel germline factor, Gone early (Goe), limits the fraction of PGCs that initiate gametogenesis. goe encodes a non-peptidase homologue of the Neprilysin family metalloendopeptidases. At the onset of gametogenesis, Goe was localized on the germ cell membrane in the ovary, suggesting that it functions in a peptidase-independent manner in cell-cell communication at the cell surface. Overexpression of Goe in the germline decreased the number of PGCs that enter the gametogenic pathway, thereby increasing the proportion of undifferentiated PGCs. Inversely, depletion of Goe increased the number of PGCs initiating differentiation. Excess PGC differentiation in the goe mutant was augmented by halving the dose of argos, a somatically expressed inhibitor of @entity11735 signaling. This increase in PGC differentiation resulted in a massive decrease in the number of undifferentiated PGCs, and ultimately led to insufficient formation of GSCs. Thus, acting cooperatively with a somatic regulator of @entity11735 signaling, the germline factor goe plays a critical role in securing the proper size of the GSC precursor pool. Because goe can suppress @entity11735 signaling activity and is expressed in EGF-producing cells in various tissues, goe may function by attenuating @entity11735 signaling, and thereby affecting the stromal environment.

gone early, a Novel Germline Factor, Ensures the Proper Size of the Stem Cell Precursor Pool in the XXXX Ovary.

@entity238 :: ('7227', 'Species') :: ['Drosophila'] @entity11735 :: ('37455(Tax:7227)', 'Gene') :: ['EGFR', 'epidermal growth factor receptor']
@entity238 :: (7227,Species) :: ['Drosophila']
