Platelet studies were done in healthy male volunteers and in @entity882 @entity1 . Some of the platelets used in the study were isolated by mechanical apheresis using either the Haemonetics blood processor 30, the IBM blood processor 2997 or the Fenwal CS-3000 blood processor before freezing. Other platelets were isolated from individual units of whole blood and pooled before freezing. The platelets were frozen with a 6% cryoprotectant ( @entity3733 ) in a @entity2634 ( @entity2634 ) plastic bag or a @entity29164 plastic bag at -80 degrees C in a mechanical freezer and stored for as long as 3 years. Some of the frozen platelets were transported in dry ice in @entity852 foam containers to determine whether they would be adversely affected by such treatment. Platelet recovery after freezing, thawing and washing was about 75%. In the healthy male volunteers, in vivo recovery of autologous platelets 1-2 h after transfusion was about 33%, and the life span was about 8 days. In the @entity882 @entity1 , in vivo recovery values were 50% of those from fresh platelets. The transfusion of previously frozen washed platelets reduced clinical @entity548 in the @entity882 @entity1 with @entity548 . There was no evidence of @entity5122 in platelets after storage at -80 degrees C for at least 2 years, as determined from in vivo recovery and in vivo survival values, nor was there any adverse effect as a result of shipment of the frozen platelets in dry ice in @entity852 foam containers from one facility to another.

Cryopreservation of @entity1 platelets using 6% XXXX and storage at -80 degrees C. Effects of 2 years of frozen storage at -80 degrees C and transportation in dry ice.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity29164 :: ('MESH:C035051', 'Chemical') :: ['polyolefin'] @entity852 :: ('MESH:D011137', 'Chemical') :: ['polystyrene'] @entity882 :: ('MESH:D013921', 'Disease') :: ['thrombocytopenic'] @entity548 :: ('MESH:D006470', 'Disease') :: ['bleeding'] @entity2634 :: ('MESH:D011143', 'Chemical') :: ['PVC', 'polyvinylchloride'] @entity5122 :: ('MESH:C565430', 'Disease') :: ['quality deterioration'] @entity3733 :: ('MESH:D004121', 'Chemical') :: ['DMSO']
@entity3733 :: (MESH:D004121,Chemical) :: ['dimethyl sulfoxide']
