The embryo defective (emb) mutants in @entity2829 genetically define a unique class of loci that is required for embryogenesis but not endosperm development, allowing dissection of two developmental processes of seed formation. Through characterization of the @entity27109 mutant, we report here that @entity27109 gene encodes a circular permuted, YqeH class GTPase protein that likely functions in 30S ribosome formation in plastids. Loss of @entity27109 function in the null mutant arrests embryogenesis at the early transition stage. @entity27109 was cloned by transposon tagging and was confirmed by analysis of four alleles. Subcellular localization indicated that the @entity27109 is targeted to chloroplasts. Recombinant @entity27109 is shown to hydrolyze @entity3007 in  vitro (Km   =  2.42      0.3   m). @entity27109 was constitutively expressed in all tissues examined and high level of expression was found in transition stage embryos. Comparison of @entity27109 and WT indicated that loss of @entity27109 function severely impairs accumulation of 16S rRNA and several plastid encoded ribosomal genes. We show that an @entity27109 transgene complements the pale green, slow growth phenotype conditioned by mutations in @entity28316 , a closely related YqeH GTPase of @entity958 . Taken together, we propose that the @entity27109 / @entity28316 /YqeH class GTPases function in assembly of the 30S subunit of the chloroplast ribosome, and that this function is essential to embryogenesis in plants.

Embryo defective 14 encodes a plastid-targeted cGTPase essential for embryogenesis in XXXX .

@entity3007 :: ('MESH:D006160', 'Chemical') :: ['GTP'] @entity2829 :: ('4577', 'Species') :: ['maize'] @entity27109 :: ('844347(Tax:3702)', 'Gene') :: ['EMB14', 'Emb14', 'emb14'] @entity958 :: ('3702', 'Species') :: ['Arabidopsis'] @entity28316 :: ('823899(Tax:3702)', 'Gene') :: ['AtNOA1']
@entity2829 :: (4577,Species) :: ['maize']
