BACKGROUND: Over the last decade, @entity5 -specific antigens have been discovered, but so far it has not been possible to use them as part of an effective acquired immunotherapy. This failure may be due to the fact that the expression of the MHC class 1 is low and in @entity2 cells is heterogeneous. Therefore, it may be advantageous to develop techniques that activate the antitumor mechanism of the innate immune system. An experimental model was developed for testing @entity2 therapies that are based on the stimulation of macrophages, which then activate innate immunity. MATERIALS AND METHODS: A549, a @entity1 @entity2220 cell line, was co-cultured with a @entity35 macrophage cell line ( @entity35 ), or a @entity1 macrophage cell line ( @entity1357 ) at the ratios of 1:1 or 1:5. The experiments were performed with lipopolysaccharide (LPS) or in its absence. The @entity137 rate to A549 cells was estimated over time using a dye-uptake method and the amount of @entity880 dehydrogenase released was measured. The amount of @entity842 (NO) induced in the medium was assayed, because it may be a candidate as a useful cytotoxic factor. RESULTS: High @entity137 was observed to A549 cells when co-cultured with @entity35 cells in the presence of LPS. This effect was not observed in the absence of LPS. Similar results, although to a lesser extent, were observed when A549 cells were co-cultured with THP-1 cells. A high concentration of NO was measured in the co-culture medium of A549 cells and @entity35 cells when LPS was present. CONCLUSION: The induction of cell death in @entity2 cells occurred after contact with macrophages that had been activated by LPS. The NO that was produced by macrophages in response to LPS was responsible for some of this effect.

Innate-immune therapy for XXXX based on tissue-macrophage activation with lipopolysaccharide.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1357 :: ('2736', 'Gene') :: ['THP 1'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung cancer'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity35 :: ('10116', 'Species') :: ['rat', 'NR8383'] @entity2220 :: ('MESH:C538231', 'Disease') :: ['lung adenocarcinoma']
@entity2 :: (MESH:D008175,Disease) :: ['lung carcinoma']
