A newly isolated mutant, mus-23, of @entity1980 was found to be highly sensitive to a wide variety of mutagens, including UV light, @entity10842 , @entity1978 , @entity2482 and @entity2469 . This mutant was originally isolated as a mutant that could not grow on medium containing @entity3793 . Meiosis and sporulation were defective in homozygous crosses between mus-23 haploids. The mus-23 gene is located on the right arm of LGII, between fl and trp-3. Analyses of epistasis between mus-23 and other mutations that cause defects in DNA repair indicated that the mus-23 gene belongs to the same DNA repair group as mei-3, which is the @entity1980 homolog of the @entity375 gene @entity11023 . The double mutant carrying mus-23 and uvs-3 mutations was lethal. The mus-23 gene was cloned by complementation of the MMS-sensitive phenotype of the mus-23 mutant. The gene contained an open reading frame of 1578 bp and did not contain any introns. The molecular weight of the predicted mus-23 gene product was 60.4 kDa. Computer analyses revealed that the MUS23 protein has significant homology to @entity42182 , which is known to be involved in recombinational repair in @entity375 . The level of mus-23 transcripts increased significantly within 60 min of treatment with UV or MMS and then gradually decreased. The role of MUS23 protein in recombinational repair is discussed.

Genetic and molecular characterization of XXXX mus-23: a gene involved in recombinational repair.

@entity2482 :: ('MESH:D008769', 'Chemical') :: ["N-methyl-N'-nitro-N-nitrosoguanidine"] @entity10842 :: ('MESH:D008741', 'Chemical') :: ['methyl methanesulfonate'] @entity1978 :: ('MESH:D015112', 'Chemical') :: ['4-nitroquinoline 1-oxide'] @entity11023 :: ('856831(Tax:4932)', 'Gene') :: ['RAD51'] @entity42182 :: ('855264(Tax:4932)', 'Gene') :: ['Mre11p'] @entity3793 :: ('CHEBI:27570', 'Chemical') :: ['histidine'] @entity1980 :: ('5141', 'Species') :: ['Neurospora', 'Neurospora crassa'] @entity2469 :: ('MESH:D020122', 'Chemical') :: ['tert-butyl hydroperoxide'] @entity375 :: ('4932', 'Species') :: ['Saccharomyces cerevisiae', 'S. cerevisiae']
@entity1980 :: (5141,Species) :: ['Neurospora crassa']
