OBJECTIVE: Determine whether peritoneal macrophages from @entity1 with @entity46 -associated @entity249 express more inducible @entity842 synthase ( @entity3623 ) and produce more NO than fertile controls. DESIGN: Unblinded clinical study. @entity1 (S): Nine infertile @entity1 with @entity46 and nine normal fertile @entity1 undergoing laparoscopy. INTERVENTION(S): Peritoneal fluid and macrophages were collected. Cells were also cultured with the @entity3623 inducers interferon-alpha ( @entity3027 ) or @entity379 plus lipopolysaccharide (LPS). MAIN OUTCOME MEASURE(S): Peritoneal fluid NO levels, peritoneal macrophage NOS activity, and peritoneal macrophage @entity3623 protein expression. RESULT(S): NOS enzyme activity was higher in peritoneal macrophages from @entity46 @entity1 . Immunoblots demonstrated @entity3623 protein only in peritoneal macrophages from @entity1 with @entity46 . Peritoneal fluid NO concentration was similar in the two groups, but total peritoneal fluid NO content was higher in @entity46 @entity1 . After 3 days' culture, peritoneal macrophages from @entity1 with @entity46 produced more NO in response to @entity3027 or @entity379 plus LPS than controls. CONCLUSION(S): Peritoneal macrophages from @entity1 with @entity46 -associated @entity249 express higher levels of @entity3623 , have higher NOS enzyme activity, and produce more NO in response to immune stimulation in vitro. As high levels of NO adversely affect sperm, embryos, implantation, and oviductal function, reducing peritoneal fluid NO production or blocking NO effects may improve fertility in @entity1 with @entity46 .

Inducible @entity842 synthase expression by peritoneal macrophages in @entity46 -associated XXXX .

@entity1 :: ('9606', 'Species') :: ['women', 'patients', 'PATIENT'] @entity249 :: ('MESH:D007246', 'Disease') :: ['infertility'] @entity46 :: ('MESH:D004715', 'Disease') :: ['endometriosis'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity379 :: ('3458', 'Gene') :: ['IFN-gamma'] @entity3623 :: ('4843', 'Gene') :: ['NOS2'] @entity3027 :: ('3439', 'Gene') :: ['IFN-alpha']
@entity249 :: (MESH:D007246,Disease) :: ['infertility']
