The coding regions of 28 entries of hexaploid @entity3505 @entity70377 genes, gene fragments or pseudogenes in GenBank were used for @entity377 alignment. These sequences could be divided into nine subgroups based on @entity377 variation. The chromosomal locations of five of the seven unassigned subgroups were identified through subgroup-specific polymerase chain reactions (PCR) using Chinese Spring group-1 nulli-tetrasomic lines. Multiple single @entity377 polymorphisms (SNPs) and small insertions/deletions were identified in each subgroup. With further mining from @entity3505 expressed sequence tag databases and targeted DNA sequencing, two SNPs were confirmed and one SNP was discovered for genes at the Gli-A1, Gli-B1 and Gli-D1 loci. A modified allele-specific PCR procedure for assaying SNPs was used to generate dominant DNA markers based on these three SNPs. For each of these three SNPs, two allele-specific primer sets were used to test Chinese Spring and 52 commercial Australian @entity3505 varieties representing a range of low-molecular-weight (LMW) alleles. PCR results indicated that all were positive with one of the primer sets and negative with the other, with the exception of three varieties containing the 1BL/1RS chromosomal translocation that were negative for both. Furthermore, markers GliA1.1, GliB1.1 and GliD1.1 were found to be correlated with @entity367 -A3 a, b or c, @entity367 -B3 b, c, d or e and @entity367 -D3 a, b or e LMW glutenin alleles, respectively. Markers GliA1.2, GliB1.2 and GliD1.2 were found to be correlated with the @entity367 -A3 d or e, @entity367 -B3 a, g or h and @entity367 -D3 c alleles, respectively. These results indicated that the @entity70377 SNP markers could be used for detecting linked LMW glutenin subunit alleles that are important in determining the quality attributes of @entity3505 products.

Identification of SNPs and development of allele-specific PCR markers for @entity70377 alleles in XXXX .

@entity3505 :: ('4565', 'Species') :: ['wheat'] @entity377 :: ('CHEBI:36976', 'Chemical') :: ['nucleotide'] @entity70377 :: ('542850(Tax:4565)', 'Gene') :: ['gamma-gliadin'] @entity367 :: ('CHEBI:18237', 'Chemical') :: ['Glu']
@entity3505 :: (4565,Species) :: ['Triticum aestivum']
