In pancreatic islet transplantation, revascularization is crucial for the graft's survival and function. In this study, the endothelium of isolated islets and revascularization and function of islet isografts in @entity6 @entity35 were investigated. Islets were isolated from Lewis @entity35 by collagenase digestion method and were examined using immunohistochemistry ( @entity18518 stain) on days 0, 1, 3, and 7 after isolation. The number of @entity18518 -positive cells in these isolated islets was counted (mean +/- SD %). Isografts (freshly isolated islets: group A, and islets cultured for 7 days: group B) transplanted in the renal subcapsule of @entity3839 -induced @entity6 Lewis @entity35 were examined using immunohistochemistry ( @entity18518 stain) on days 3, 5, and 7 after transplantation. Intravenous @entity413 tolerance tests (IVGTT) were performed on days 3 and 7 after transplantation. The number of @entity18518 -positive cells in the isolated islets on days 0, 1, 3, and 7 after isolation were: 17.3 +/- 4.1%, 8.2 +/- 0.7%, 2.1 +/- 0.8%, and 0.8 +/- 0.5%, respectively (p < 0.05). On day 5 after transplantation, @entity18518 -positive cells were not detected in group A and B grafts, but were detected in both groups in periphery of the islets. On day 7, @entity18518 -positive microvessels were present throughout the entire graft. IVGTT values in groups A and B on days 3 and 7 after transplantation did not show significant differences. In renal subcapsular isografts in @entity6 @entity35 , revascularization into islet grafts occurs from the surrounding host tissue 5 days after transplantation, but has no influence on the response to @entity413 during this period.

Revascularization and function of pancreatic islet isografts in @entity6 XXXX following transplantation.

@entity18518 :: ('29583(Tax:10116)', 'Gene') :: ['CD31'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetic'] @entity3839 :: ('MESH:D013311', 'Chemical') :: ['streptozotocin'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity35 :: (10116,Species) :: ['rats']
