We previously reported significant relationships between sialyl Lewis antigen expression on @entity356 cells and both hepatic @entity3 and clinical prognosis. The purpose of this study was to compare the expression of sialyl Lewis antigens on @entity356 cells to elucidate the possible role of sialyl Lewis antigens in predicting the spread of a @entity5 with regard to histological findings. Subjects consisted of 38 @entity1 with @entity356 . For comparison we measured the values for sialyl Lewisa (sLea) and sialyl Lewisx (sLex) expression on the surface of about 10,000-30,000 @entity5 cells. Monoclonal antibodies CA19-9 and KM-93 were used to determine the frequency (%) and quantity (channel) of the expression by flow cytometry. We assessed the correlation of sLea and sLex expression with histological findings (depth of @entity5 invasion (pT), lymphatic invasion (ly), venous invasion (v), and lymph node @entity3 (pN)), by comparing sLea and sLex expression in relation to the grade of histological findings. A significant relationship was found between lymphatic invasion and the frequency of sLea expression (r = 0.40, p<0.05). The mean values of sLea frequency in cases categorized as ly 2 (36.30) and ly 3 (31.81) were statistically higher than those in ly 1 cases (12.74) (p<0.05). A significant relationship was also observed between lymph node @entity3 and the frequency of sLea expression (r = 0.46, p<0.01). The mean value of sLea frequency in @entity8603 cases (44.14) was statistically higher than those in pN 0 (17.11) and @entity3121 (19.03) cases (p<0.05). Neither the depth of @entity5 invasion nor venous invasion showed any correlation with the expression of sialyl Lewis antigens. In conclusion the frequency of sLea expression on the surface of @entity356 cells was greater in those @entity1 who developed lymphatic invasion and lymph node @entity3 . However, the mechanism by which sialyl Lewisa expression is upregulated remains unclear.

The clinical significance of sialyl Lewis antigen expression in the spread of XXXX . Flow cytometric DNA analysis.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity8603 :: ('6336', 'Gene') :: ['pN 3'] @entity3121 :: ('5270', 'Gene') :: ['pN 1'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity356 :: ('MESH:D013274', 'Disease') :: ['gastric cancer']
@entity356 :: (MESH:D013274,Disease) :: ['gastric cancer']
