Cytotoxicities of a group of frequently used organic solvents were assessed by their effect on thermal stability of erythrocyte membrane proteins. The denaturation temperatures Tm of membrane proteins, peripheral and intrinsic, were detected by the increase in the derivative of suspension impedance during heating. These Tm linearly changed by delta Tm in the presence of organic solvents indicating labilization (negative delta Tm) or stabilization (positive delta Tm) of the structure of respective membrane protein. The potency P of the solvent with molar concentration Cex to affect the conformation stability of membrane protein was defined as delta Tm/Cex. This potency decreased as both polarity of solvent and its capability to form @entity3884 bonds increased. In some solvents ( @entity3733 and @entity6442 ) the potencies to destabilized peripheric and intrinsic proteins were equal. @entity27390 destabilized selectively peripheral proteins. Some solvents ( @entity653 , especially @entity20704 ) stabilised thermally proteins. As the hydrophobicity of the solvents increased ( @entity10977 , @entity1042 , @entity1068 , @entity1324 , @entity4605 , @entity1046 , @entity43840 ) the potency for destabilization of intrinsic proteins strongly increased. Thus, the use of more polar solvents capable of forming more @entity3884 bonds appears preferable when low @entity137 should be attained.

Rapid method for comparing the XXXX of organic solvents and their ability to destabilize proteins of the erythrocyte membrane.

@entity4605 :: ('MESH:C023666', 'Chemical') :: ['pyridine'] @entity3884 :: ('MESH:D006859', 'Chemical') :: ['hydrogen'] @entity1046 :: ('MESH:C007650', 'Chemical') :: ['ethyl acetate'] @entity10977 :: ('MESH:D019855', 'Chemical') :: ['ethylene glycol'] @entity43840 :: ('MESH:C014476', 'Chemical') :: ['diethyl maleate'] @entity1042 :: ('MESH:D000432', 'Chemical') :: ['methanol'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity27390 :: ('MESH:C031066', 'Chemical') :: ['Formamide'] @entity1324 :: ('MESH:D000096', 'Chemical') :: ['acetone'] @entity653 :: ('MESH:D005990', 'Chemical') :: ['glycerol'] @entity3733 :: ('MESH:D004121', 'Chemical') :: ['dimethyl sulfoxide'] @entity6442 :: ('MESH:D004126', 'Chemical') :: ['dimethyl formamide'] @entity20704 :: ('MESH:D004896', 'Chemical') :: ['erythritol'] @entity1068 :: ('MESH:D000431', 'Chemical') :: ['ethanol']
@entity137 :: (MESH:D064420,Disease) :: ['cytotoxicity']
