UNASSIGNED: Mitochondria are not only the main source of energy in cells but also produce reactive @entity26 species (ROS), which result in oxidative stress when in space. This oxidative stress is responsible for energy imbalances and cellular damage. In this study, a @entity35 tail suspension model was used in individual experiments for 7 and 21 days to explore the effect of simulated microgravity (SM) on metabolic proteins in the hippocampus, a vital brain region involved in learning, memory, and navigation. A comparative @entity14682 -labeled quantitative proteomic strategy was used to observe the differential expression of metabolic proteins. Forty-two and sixty-seven mitochondrial metabolic proteins were differentially expressed after 21 and 7 days of SM, respectively. Mitochondrial Complex I, III, and IV, @entity9275 dehydrogenase and @entity5910 dehydrogenase were down-regulated. Moreover, @entity68939 , which defend against oxidative damage, were up-regulated in the hippocampus. Western blot analysis of proteins @entity37128 and @entity68940 confirmed the mass spectrometry results. Despite these changes in mitochondrial protein expression, no obvious cell apoptosis was observed after 21 days of SM. The results of this study indicate that the oxidative stress induced by SM has profound effects on metabolic proteins.

Effect of Prolonged Simulated Microgravity on Metabolic Proteins in XXXX Hippocampus: Steps toward Safe Space Travel.

@entity37128 :: ('117287(Tax:10116)', 'Gene') :: ['DJ-1'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity14682 :: ('CHEBI:33815', 'Chemical') :: ['(18)O'] @entity5910 :: ('MESH:C030298', 'Chemical') :: ['malate'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity68939 :: ('117287;94167', 'Gene') :: ['DJ-1 and peroxiredoxin 6'] @entity9275 :: ('MESH:C034219', 'Chemical') :: ['isocitrate'] @entity68940 :: ('252934(Tax:10116)', 'Gene') :: ['COX 5A']
@entity35 :: (10116,Species) :: ['Rat']
