Association of @entity69828 ( @entity69828 ) to cells via binding to its specific cellular receptor ( @entity69829 ) augments the potential of these cells to support plasminogen activation, a process that has been implicated in the degradation of extracellular matrix proteins during cell migration and tissue remodeling. The @entity69828 receptor is a glycolipid-anchored membrane protein belonging to the Ly-6/ @entity69829 superfamily and is the only multidomain member identified so far. We have now purified the three individual domains of a recombinant soluble @entity69829 variant, expressed in @entity3569 ovary cells, after limited proteolysis using chymotrypsin and pepsin. The glycosylation patterns of these domains have been determined by matrix assisted laser desorption ionization and electrospray ionization mass spectrometry. Of the five potential attachment sites for @entity2734 -linked @entity1735 in @entity69829 only four are utilized, as the tryptic peptide derived from domain III containing @entity2734 233 was quantitatively recovered without @entity1735 . The remaining four attachment sites were shown to exhibit site-specific microheterogeneity of the @entity2734 -linked @entity1735 . The glycosylation on @entity2734 52 (domain I) and @entity2734 172 (domain II) is dominated by the smaller biantennary complex-type oligosaccharides, while @entity2734 162 (domain II) and @entity2734 200 (domain III) predominantly carry tri- and tetraantennary complex-type oligosaccharides. The @entity1735 moiety on @entity2734 52 in @entity69829 domain I could be selectively removed by N-glycanase treatment under nondenaturing conditions. This susceptibility was abrogated when @entity69829 participitated in a bimolecular complex with pro- @entity69828 or smaller receptor binding derivatives thereof, demonstrating the proximity of the ligand-binding site to this particular @entity1735 moiety. @entity69829 preparations devoid of @entity1735 on domain I exhibited altered binding kinetics toward @entity69828 (a 4-6-fold increase in Kd) as assessed by real time biomolecular interaction analysis.

Glycosylation profile of a recombinant XXXX receptor expressed in @entity3569 ovary cells.

@entity69828 :: ('100759384', 'Gene') :: ['uPA', 'urokinase-type plasminogen activator'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['carbohydrate'] @entity2734 :: ('CHEBI:17196', 'Chemical') :: ['asparagine', 'Asn'] @entity69829 :: ('100689422', 'Gene') :: ['uPAR'] @entity3569 :: ('10029', 'Species') :: ['Chinese hamster']
@entity69828 :: (100759384,Gene) :: ['urokinase-type plasminogen activator']
