@entity1 @entity40330 ( @entity30946 ) mRNA level and promoter activity increase when keratinocytes are treated with the differentiating agent, @entity864 ( @entity865 ). This response is mediated via a p38 mitogen-activated protein kinase-dependent pathway that targets @entity23601 (Efimova, T., LaCelle, P. T. , Welter, J. F., and Eckert, R. L. (1998) J. Biol. Chem. 273, 24387-24395). In the present study we examine the role of various PKC isoforms in this regulation. Transfection of expression plasmids encoding the novel @entity69068 increase @entity30946 promoter activity. In contrast, neither conventional @entity10185 ) nor the atypical isoform (zeta) regulate promoter activity. Consistent with these observations, promoter activity is inhibited by the @entity4456 -selective inhibitor, @entity12703 , but not by @entity9295 , an inhibitor of conventional PKC isoforms, and novel PKC isoform-dependent promoter activation is inhibited by dominant-negative @entity4456 . This regulation appears to be physiologically important, as transfection of keratinocytes with @entity4456 , -epsilon, or -eta increases expression of the endogenous @entity30946 gene. Synergistic promoter activation (>/=100-fold) is observed when PKCepsilon- or -eta-transfected cells are treated with @entity865 . In contrast, the @entity4456 -dependent response is more complex as either activation or inhibition is observed, depending upon @entity4456 concentration.

Regulation of XXXX @entity40330 promoter activity by novel protein kinase C isoforms.

@entity1 :: ('9606', 'Species') :: ['Human'] @entity9295 :: ('MESH:C081021', 'Chemical') :: ['Go-6976'] @entity12703 :: ('MESH:C085746', 'Chemical') :: ['rottlerin'] @entity10185 :: ('5578', 'Gene') :: ['PKC isoforms (alpha, beta, and gamma'] @entity69068 :: ('5580;5581', 'Gene') :: ['PKC isoforms delta, epsilon, and eta'] @entity23601 :: ('3727', 'Gene') :: ['activator protein 1'] @entity30946 :: ('27130', 'Gene') :: ['hINV'] @entity865 :: ('CHEBI:61432', 'Chemical') :: ['TPA'] @entity4456 :: ('5580', 'Gene') :: ['PKCdelta'] @entity864 :: ('MESH:C554292|MESH:D013755', 'Chemical') :: ['12-O-tetradecanoylphorbol-13-acetate'] @entity40330 :: ('3713', 'Gene') :: ['involucrin']
@entity1 :: (9606,Species) :: ['human']
