The participation of reactive @entity26 species (ROS) in luminescence (chemiluminescence and autofluorescence induced by ultraviolet light of 360-380 nm) was analyzed. Microspores, the pollen (male gametophyte) of Hippeastrum hybridum, Philadelphus grandiflorus, and Betula verrucosa and vegetative microspores of the spore-breeding plant @entity26360 served as models. It was found that the addition of the chemiluminescent probe @entity12487 , which luminesces in the presence of @entity224 anionradicals, leads to intensive chemiluminescence of microspores. No emission was observed in the absence of @entity12487 and in the presence of the dye @entity6931 as a chemiluminescent probe. The emission decreased significantly if @entity224 dismutase, an enzyme of the @entity224 anionradical dismutation during which this radical disappeared, was added before the dye addition. The autofluorescence intensity of microspores decreased in the presence of both @entity224 dismutase and peroxidase, an enzyme destroying @entity273 and organic @entity2627 . The most significant effect was noted after the addition of peroxidase, which indicates a greater contribution of @entity2627 to this type of emission. The fumigation with @entity1787 , which increases the amount of ROS on the cell surface, enhanced the intensity of the chemiluminescence of microspores with @entity12487 , but decreased the intensity of the autofluorescence of microspores. Exogenous @entity2627 ( @entity273 and @entity2469 ) stimulated the autofluorescence of pollen and vegetative spores in a concentration-dependent manner. It was shown that the formation of ROS contributes to the luminescence of plant microspores, which reflects their functional state.

[Reactive XXXX forms and luminescence of intact microspore cells].

@entity2627 :: ('MESH:D010545', 'Chemical') :: ['peroxides'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity6931 :: ('MESH:D008165', 'Chemical') :: ['luminol'] @entity26360 :: ('3258', 'Species') :: ['Equisetum arvense'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['hydrogen peroxide'] @entity2469 :: ('MESH:D020122', 'Chemical') :: ['tert-butylhydroperoxide'] @entity224 :: ('MESH:D013481', 'Chemical') :: ['superoxide'] @entity1787 :: ('MESH:D010126', 'Chemical') :: ['ozone'] @entity12487 :: ('MESH:C033472', 'Chemical') :: ['lucigenin']
@entity26 :: (MESH:D010100,Chemical) :: ['oxygen']
