Evidence suggests that @entity880 could be a preferential energy substrate transferred from astrocytes to neurons. Such a process implies the presence of specific @entity3338 transporters on both cell types. Expression of @entity34742 and @entity58856 , two isoforms of the @entity3338 transporter (MCT) family, was studied in enriched cultures of @entity19 cortical astrocytes or neurons. It was observed that, at both the mRNA and the protein levels, astrocytes strongly expressed @entity34742 but had very little if any @entity58856 . By contrast, neurons had high amounts of @entity58856 mRNA, although @entity34742 mRNA was also detected. Double immunofluorescent labelings with appropriate markers confirmed the cell-specific preference in the expression of @entity34742 and @entity58856 , but they revealed that a subset of neurons expresses low to moderate levels of @entity34742 . Parallel immunocytochemical stainings of cultured neurons with the presynaptic marker @entity25242 showed that @entity58856 expression is correlated with synaptic development. Although @entity58856 and @entity25242 were not colocalized, their distribution was similar, and they were often closely apposed, suggesting that @entity58856 could be associated with postsynaptic terminals. Interaction between astrocytes and neurons, as occurring in layered cultures, did not modify the levels of @entity34742 and @entity58856 expression or their distribution and cell-specific preference under the conditions used. However, a close apposition between neurites and @entity34742 -expressing astrocytic processes was apparent and developed as cultures evolved. In addition to providing an extensive description of MCT distribution in cultured cells, our data underscore the potential of such preparations for future studies on the regulation of MCT expression.

Cell-specific expression pattern of @entity3338 transporters in astrocytes and neurons observed in different XXXX brain cortical cell cultures.

@entity19 :: ('10090', 'Species') :: ['mouse'] @entity25242 :: ('20977(Tax:10090)', 'Gene') :: ['synaptophysin'] @entity58856 :: ('20503(Tax:10090)', 'Gene') :: ['MCT2'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity34742 :: ('17236(Tax:10090)', 'Gene') :: ['MCT1'] @entity3338 :: ('CHEBI:35757', 'Chemical') :: ['monocarboxylate']
@entity19 :: (10090,Species) :: ['mouse']
