Exocytosis-the release of the contents of a vesicle--proceeds by two mechanisms. Full fusion occurs when the vesicle and plasma membranes merge. Alternatively, in what is termed kiss-and-run, vesicles can release transmitter during transient contacts with the plasma membrane. Little is known at the molecular level about how the choice between these two pathways is regulated. Here we report amperometric recordings of @entity2922 efflux through individual fusion pores. Transfection with @entity22880 ( @entity22880 ) IV increased the frequency and duration of kiss-and-run events, but left their amplitude unchanged. Endogenous @entity67934 , induced by @entity1928 treatment, had a similar effect. Full fusion was inhibited by mutation of a @entity141 ligand in the @entity10363 domain of @entity22880 ; kiss-and-run was inhibited by mutation of a homologous @entity141 ligand in the C2B domain of @entity67934 . The @entity141 sensitivity for full fusion was 5-fold higher with @entity22880 than @entity67934 , but for kiss-and-run the @entity141 sensitivities differed by a factor of only two. @entity22880 thus regulates the choice between full fusion and kiss-and-run, with @entity141 binding to the @entity10363 and C2B domains playing an important role in this choice.

Different domains of XXXX control the choice between kiss-and-run and full fusion.

@entity22880 :: ('6857', 'Gene') :: ['Syt', 'synaptotagmin', 'Syt I'] @entity10363 :: ('c|SUB|C|2|A', 'DNAMutation') :: ['C2A'] @entity67934 :: ('6860', 'Gene') :: ['Syt IV'] @entity2922 :: ('CHEBI:33567', 'Chemical') :: ['catecholamine'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+'] @entity1928 :: ('MESH:D005576', 'Chemical') :: ['forskolin']
@entity22880 :: (6857,Gene) :: ['synaptotagmin']
