@entity2247 ( @entity2247 .) that had been malted for 5 d developed only a small amount of bound (inactive) limit dextrinase, and very little free (active) enzyme was detected. Continuation of malting for up to 10 d only slightly increased the amount of both bound and free forms. Grain grown under conditions of ample moisture (wet grown) for 5 d produced a much higher amount of bound enzyme but a similarly low amount of free enzyme compared to malting conditions. After 10 d of growth there was a decrease in the amount of bound enzyme and a large increase in the amount of free enzyme, such that almost all of the enzyme was present in the free form. A more detailed study of limit dextrinase development in wet-grown grains revealed that a bound form was rapidly produced soon after germination. Five to 6 d after germination the amount of bound enzyme decreased rapidly and a very low amount was found in grains 9 d after germination. Meanwhile, a free form appeared slightly later and its initial rate of development was slow. At about 5 d after germination, precisely when the bound enzyme began to decrease, the free form increased rapidly, so that by 9 d after germination nearly all the enzyme was in the free form. The release of bound limit dextrinase in vitro occurred by proteolytic modification through the action of @entity462 proteinases that were kept active or activated by the presence of reduced @entity1433 in the extraction medium. The presence of @entity462 proteinases was confirmed by inhibition studies using the inhibitors @entity8867 , @entity8816 , antipain, and leupeptin. In addition, most of the bound form of limit dextrinase was soluble in 0.2 M @entity13101 buffer (pH 5.0) following extraction at 30[deg]C for 16 h and centrifugation at 3000g.

Development of Limit Dextrinase in Germinated XXXX ( @entity2247 .) (Evidence of Proteolytic Activation).

@entity13101 :: ('MESH:D019346', 'Chemical') :: ['sodium acetate'] @entity1433 :: ('MESH:D013438', 'Chemical') :: ['thiols'] @entity2247 :: ('4513', 'Species') :: ['Barley', 'Hordeum vulgare L'] @entity462 :: ('MESH:D003545', 'Chemical') :: ['cysteine'] @entity8867 :: ('MESH:D007460', 'Chemical') :: ['iodoacetamide'] @entity8816 :: ('MESH:D005033', 'Chemical') :: ['N-ethylmaleimide']
@entity2247 :: (4513,Species) :: ['Barley']
