The ability of @entity1707 to neutralize the reactive @entity26 species (ROS) plays a key role in their welfare. Two micromethods were developed to evaluate the oxidative stress and the anti-oxidative response in sera. We tested these methods for linearity, repeatability and reproducibility. A @entity3787 radicals (HR) test, based on the Fenton reaction, showed a linearity between 0.26 and 4.5 mM @entity274 (r = 0.997), with a coefficient of repeatability (CVr) of 1.9 and a coefficient of reproducibility (CVR) of 2.9. An @entity66 ( @entity66 ) test, based on the capability of sera to neutralize a titred @entity9784 solution ( @entity9785 ), showed a linearity between 55 and 880 microM @entity9785 neutralized (r = 0.984), with CVr = 3.1 and CVR = 4.9. We assessed the applicability of such tests in field conditions on three different farms. Farm A recorded a positive anamnesis of @entity299 . Dietary @entity270 supplementation was given at 50 p.p.m. in farms A and B and at 150 p.p.m. in farm C, respectively. The group from farm A showed HR sera levels higher than those of farm B and farm C (farm A, mean = 31.61 +/- 2.87 mM @entity274 , n = 10; farm B, 28.53 +/- 2.30, n = 10; and farm C, 25.63 +/- 1.03, n = 10; P < 0.01). The @entity66 test was not able to discriminate between farms. The @entity66 levels found (mean = 472.50 microM @entity9785 neutralized: n = 30) probably represent the maximum response of the organism to compensate the oxidative stress. HR and @entity66 are stable in sera stored at +4 degrees C. We discuss the application of such methods for the assessment of farmed animals' welfare.

Evaluation of the oxidative stress in growing XXXX by microplate assays.

@entity1707 :: ('9823', 'Species') :: ['pigs'] @entity9784 :: ('MESH:D006997', 'Chemical') :: ['hypochlorous acid'] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H2O2'] @entity66 :: ('MESH:D004194', 'Disease') :: ['anti-oxidant power', 'AOP'] @entity270 :: ('MESH:D014810', 'Chemical') :: ['Vitamin E'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity9785 :: ('CHEBI:24757', 'Chemical') :: ['HClO'] @entity3787 :: ('MESH:D017665', 'Chemical') :: ['hydroxyl'] @entity299 :: ('MESH:D006331', 'Disease') :: ['Mulberry heart disease']
@entity1707 :: (9823,Species) :: ['pigs']
