@entity2227 is transported into synaptic vesicles by vesicular @entity2227 transporter (VGLUT) proteins. Three different VGLUTs, @entity3862 , @entity3863 , and @entity50334 , have recently been characterized, and they are considered to represent the most specific marker so far for neurons using @entity2227 as transmitter. We analyzed the cellular localization of @entity3862 -3 in the @entity35 spinal cord and dorsal root ganglia (DRGs) in control @entity35 and after dorsal rhizotomy. Using in situ hybridization, @entity3862 mRNA containing neurons were shown in the dorsomedial part of the intermediate zone, whereas @entity3863 mRNA-expressing neurons were present in the entire intermediate zone, both populations most likely representing interneurons. @entity50334 mRNA could not be detected in the spinal cord. In the ventral horn, a dense plexus of @entity3862 -immunoreactive (ir) nerve terminals was present, with large varicosities abutting on presumed motoneurons. In the dorsal horn a similarly dense plexus was seen, except in laminae I and II. A very dense plexus of @entity3863 -ir fibers was distributed in the entire gray matter of the spinal cord, with many fibers lying close to presumed motoneurons. Few @entity50334 -ir fibers were distributed in the white and gray matter, including lamina IX. However, a dense @entity50334 -ir plexus was seen in the sympathetic intermedio-lateral column (IML). Multiple-labeling immunohistochemistry revealed that the @entity3862 -, @entity3863 -, and @entity2579 -containing varicosities in lamina IX all represent separate entities. There was no colocalization of @entity50334 with @entity2579 or @entity750 in varicose fibers of the ventral horn, but some @entity50334 -ir fibers in the IML were @entity750 -positive. Lesioning of the dorsal roots resulted in an almost complete disappearance of @entity3862 -ir fibers around motoneurons and a less pronounced decrease in the remaining gray matter, whereas the density of @entity3863 - and @entity2579 -ir fibers appeared unaltered after lesion. Many @entity3862 -ir neurons were observed in DRGs; they were almost all large and did not colocalize @entity4655 ( @entity4655 ), and there was no overlap between these markers in fibers in the superficial dorsal horn. @entity3863 was, at most, seen in a few DRG neurons. Taken together, these results suggest that the VGLUTs mRNAs are present in distinct subsets of neuronal populations at the spinal level. @entity3862 is mainly present in primary afferents from large, @entity4655 -negative DRG neurons, @entity3863 has mainly a local origin, and @entity50334 fibers probably have a supraspinal origin.

Cellular localization of three vesicular @entity2227 transporter mRNAs and proteins in XXXX spinal cord and dorsal root ganglia.

@entity2579 :: ('60422(Tax:10116)', 'Gene') :: ['VAChT'] @entity2227 :: ('CHEBI:29987', 'Chemical') :: ['glutamate', 'Glutamate'] @entity3862 :: ('116638(Tax:10116)', 'Gene') :: ['VGLUT1'] @entity50334 :: ('266767(Tax:10116)', 'Gene') :: ['VGLUT3'] @entity750 :: ('MESH:D012701', 'Chemical') :: ['5-HT'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity3863 :: ('84487(Tax:10116)', 'Gene') :: ['VGLUT2'] @entity4655 :: ('24241(Tax:10116)', 'Gene') :: ['CGRP', 'calcitonin gene-related peptide']
@entity35 :: (10116,Species) :: ['rat']
