We have evaluated the effect of @entity5870 on the protein expression of microsomal and cytosolic @entity1421 synthases ( @entity8207 and @entity10326 ) in preterm @entity1 placentae (PL) and fetal membranes (FM), by Western blot and immunohistochemistry, as well as the regulatory effect of @entity1356 and @entity310 on @entity8207 , @entity10326 , and @entity2247 expression in @entity3567 ( @entity3567 ) and @entity3567 ( @entity3567 ) cell cultures. @entity8207 localized to the syncytiotrophoblast and vascular endothelium in PL and to the amnion epithelium, @entity3567 , and decidual cells in FM. @entity10326 protein was localized only to the syncytiotrophoblast in PL and had the same profile of expression as @entity8207 in FM. With @entity281 , there was an increase in @entity8207 expression in PL and a decrease in the expression in FM. @entity10326 protein did not change in either PL or FM with @entity281 . In @entity3567 cells in culture, @entity1356 up-regulated @entity2247 protein expression but did not affect @entity8207 . However, @entity310 increased both @entity8207 and @entity2247 protein expression in these cells. In @entity3567 cells in culture, @entity1356 and @entity310 increased both @entity8207 and @entity2247 protein levels. Neither @entity1356 nor @entity310 affected @entity10326 in either @entity3567 or @entity3567 cells. We conclude that protein levels of @entity8207 , as well as @entity2247 , can be stimulated by cytokines, potentially contributing to the increased @entity3009 production at the time of @entity3152 . However, multiple mechanisms, which apparently are inductor- and cell-type-specific, exist for the regulation of these enzymes.

Differential expression and regulation of microsomal @entity1421 synthase in @entity1 fetal membranes and placenta with XXXX and in cultured trophoblast cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity3152 :: ('MESH:D007752', 'Disease') :: ['infection-driven preterm labor'] @entity1356 :: ('3553', 'Gene') :: ['IL-1beta'] @entity310 :: ('7124', 'Gene') :: ['TNF-alpha'] @entity1421 :: ('MESH:D015232', 'Chemical') :: ['prostaglandin E(2)'] @entity5870 :: ('MESH:D002821', 'Disease') :: ['chorioamnionitis'] @entity3009 :: ('CHEBI:26333', 'Chemical') :: ['prostaglandin'] @entity10326 :: ('10728', 'Gene') :: ['cPGES'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity8207 :: ('64292(Tax:10090)', 'Gene') :: ['mPGES'] @entity3567 :: ('MESH:D014328', 'Disease') :: ['villous trophoblast', 'CT', 'chorion trophoblast', 'VT'] @entity2247 :: ('4513', 'Gene') :: ['cyclooxygenase (COX)-2', 'COX-2']
@entity281 :: (MESH:D007239,Disease) :: ['infection']
