The joining of breaks in the chromosomal DNA backbone by ligases in processes of replication, recombination and repair plays a crucial role in the maintenance of genomic stability. Four @entity855 -dependent ligases, designated DNA ligases I-IV, have been identified in higher eukaryotes, and each one has distinct functions. In mammals and @entity375 , @entity15543 is exclusively involved in the repair of DNA double-strand breaks by non-homologous end joining. Recently, an @entity958 orthologue of the @entity375 and @entity1 @entity15543 gene was found and termed @entity53625 . Here we describe the isolation and functional characterisation of a plant line with a T-DNA insertion in the @entity53625 gene. Plants homozygous for the T-DNA insertion did not display any growth or @entity245 and were fertile. However, mutant seedlings were hypersensitive to the DNA-damaging agents @entity10842 and X-rays, demonstrating that @entity53625 is required for the repair of DNA damage. Recently, we showed that a @entity375 @entity41103 mutant is deficient in Agrobacterium T-DNA integration. However, using tumorigenesis and germline transformation assays, we found that the plant @entity53625 mutant is not impaired in T-DNA integration. Thus, in contrast to @entity375 , @entity15543 is not required for T-DNA integration in plants.

The XXXX @entity53625 gene is required for the repair of DNA damage, but not for the integration of Agrobacterium T-DNA.

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity10842 :: ('MESH:D008741', 'Chemical') :: ['methyl methanesulfonate'] @entity958 :: ('3702', 'Species') :: ['Arabidopsis thaliana'] @entity245 :: ('MESH:D002658', 'Disease') :: ['developmental defects'] @entity15543 :: ('3981', 'Gene') :: ['DNA ligase IV'] @entity41103 :: ('854166(Tax:4932)', 'Gene') :: ['lig4'] @entity375 :: ('4932', 'Species') :: ['yeast'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP'] @entity53625 :: ('835822(Tax:3702)', 'Gene') :: ['AtLIG4']
@entity958 :: (3702,Species) :: ['Arabidopsis']
