PURPOSE: To evaluate flow cytometry of vitreous cellular specimens as a means of diagnosing @entity6537 and @entity2194 . METHODS: We undertook a retrospective, observational study of hematopoietic cell-surface markers in 20 @entity1 with vitreous cellular infiltration in whom @entity706 was considered in the differential diagnosis. Immunophenotyping of vitreous cells obtained by vitrectomy was performed by flow cytometry using antibodies directed against specific cell-surface antigens, including ones associated with B-lymphocyte and @entity784 and activated inflammatory cells. Smears were examined cytologically. Cytofluorography was compared with the cytopathologic diagnosis and with final diagnosis. RESULTS: With flow cytometry, a diagnosis of @entity6537 was confirmed in two of four @entity1 with known @entity706 , one of whom had recurrent disease after radiation, and not confirmed in two @entity1 who had had prior treatment with radiation or @entity288 . In six @entity1 with no prior diagnosis of @entity706 , five were diagnosed with @entity706 on the basis of cytofluorography. Thus, seven (70%) of 10 @entity1 with @entity6537 were diagnosed by cytofluorography compared with three (30%) of 10 with @entity706 diagnosed by cytology. With flow cytometry, 10 @entity1 with @entity1596 or intraocular infections were distinguishable from @entity1 with @entity706 by lack of a monotypic population and, in some cases, by elevated @entity404 : @entity405 ratios and a high percentage of activated cells. CONCLUSIONS: Cytofluorography of vitreous cells is an effective alternative or adjunct to cytology. Information can be gained from specimens that are uninterpretable by routine cytology. The optimal technique for diagnosis may vary among institutions.

Diagnosis of XXXX by flow cytometry.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity2194 :: ('MESH:D005128', 'Disease') :: ['ocular inflammatory disease'] @entity404 :: ('920', 'Gene') :: ['CD4'] @entity706 :: ('MESH:D008223', 'Disease') :: ['lymphoma'] @entity784 :: ('MESH:D016399', 'Disease') :: ['T-lymphocyte lymphomas'] @entity1596 :: ('MESH:D014605', 'Disease') :: ['uveitis'] @entity6537 :: ('MESH:D064090', 'Disease') :: ['intraocular lymphoma'] @entity405 :: ('925', 'Gene') :: ['CD8'] @entity288 :: ('MESH:D000305', 'Chemical') :: ['corticosteroids']
@entity6537 :: (MESH:D064090,Disease) :: ['intraocular lymphoma']
