PURPOSE: Retroviral transduction for genetic enhancement of endothelial cell (EC) anti- @entity715 phenotype offers potential for improving the clinical success of vascular graft seeding; however, application of this technique may bring concomitant alteration in cell functionality. METHODS: @entity1 microvascular ECs were transduced with a retroviral vector encoding for the marker gene @entity1194 . Transduced endothelial cells (rtECs) and nontransduced endothelial cells (ntECs) were evaluated by flow cytometry for expression of @entity2071 and @entity3145 ( @entity3145 ) on both smooth (coverslips) and graft (Dacron, 6 mm inside diameter) surfaces under static and shear exposed conditions. Graft EC retention was measured after 6-hour pulsatile perfusions. Platelet and neutrophil adherence was measured on perfused coverslips. RESULTS: Lower levels of @entity2071 were expressed by rtECs on coverslips under both static (p < 0.01 vs static ntECs) and shear exposed conditions (p < 0.01 vs static and shear ntECs). Accordingly, fewer polymorphonuclear leukocytes adhered to rtEC monolayers (p < 0.01 vs ntECs). No difference in @entity2071 and @entity3145 expression by static graft seeded rtECs and ntECs was observed. However, graft-seeded rtECs that were exposed to wall shear stress displayed less @entity3145 than sheared ntECs (p < 0.05). Transduction did not affect EC retention to the sheared graft surface. CONCLUSIONS: These data suggest that retroviral transduction does not elicit a prothrombotic/proinflammatory phenotype, rather indices of these states appear in some conditions to be reduced. Further, transduction does not adversely affect EC adherence to Dacron graft surfaces under arterial hemodynamics.

Effect of retroviral transduction on XXXX endothelial cell phenotype and adhesion to Dacron vascular grafts.

@entity1 :: ('9606', 'Species') :: ['Human'] @entity3145 :: ('MESH:D017695', 'Disease') :: ['TF', 'tissue factor'] @entity1194 :: ('2720', 'Gene') :: ['beta-galactosidase'] @entity2071 :: ('3383', 'Gene') :: ['ICAM-1', 'intercellular adhesion molecule (ICAM)-1'] @entity715 :: ('MESH:D013927', 'Disease') :: ['thrombotic']
@entity1 :: (9606,Species) :: ['human']
