AIM: To compare the characteristics of bioceramic endodontic sealer Endosequence BC sealer with those of AH Plus sealer. METHODOLOGY: @entity137 and genotoxicity were analysed on @entity1 @entity2522 fibroblasts submitted to cell culture medium conditioned by sealers using the @entity98 reduction assay and micronucleus formation test (MNT), respectively. Cells grown on fresh medium served as controls. Cell viabilities were measured at 1, 3, 5 and 7  days. The antibacterial activity was analysed on an @entity797 (ATCC 29212) using both on agar diffusion test (ADT) and a direct contact test (DCT). The inhibition zones in ADT were measured after 48  h and the colony-forming units counting in the DCT after 1, 24, 72 and 168  h. Data were compared by anova and Tukey's test and MNT by Fisher's exact test (P  <  0.05). RESULTS: Cultures submitted to Endosequence BC sealer had a significantly higher number of viable cells (P  <  0.01) and less micronucleus formation (P  <  0.05) than AH Plus sealer. Endosequence BC sealer exhibited significantly smaller inhibition zones (6.00     0.03  mm) than AH Plus sealer (10.31      0.21  mm) (P  <  0.05). Moreover, Endosequence BC sealer had significantly smaller antibacterial activity than AH Plus sealer up to 1  h of direct contact (P  <  0.05). On other exposure times, both materials had similar antibacterial effectiveness (P  >  0.05). CONCLUSIONS: Bioceramic-based sealer had less @entity137 and genotoxicity and similar antibacterial effect against @entity797 in comparison with AH Plus sealer.

XXXX , genotoxicity and antibacterial effectiveness of a bioceramic endodontic sealer.

@entity1 :: ('9606', 'Species') :: ['human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['Cytotoxicity', 'cytotoxicity'] @entity2522 :: ('MESH:D005891', 'Disease') :: ['gingival'] @entity797 :: ('1351', 'Species') :: ['E.  faecalis', 'Enterococcus faecalis strain'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT']
@entity137 :: (MESH:D064420,Disease) :: ['Cytotoxicity']
