BACKGROUND: Metastatic recurrence is the main cause of @entity0 . Tumour cell proliferation and migration are crucial steps in the metastatic process. Several perioperative factors, including general anaesthesia and opioid @entity1337 , adversely affect immune function, potentially increasing metastatic recurrence. Regional anaesthesia- @entity1337 has been consistently shown to attenuate the stress response to surgery, and also reduce opioid and general anaesthesia requirements, thereby attenuating this perioperative immunosuppression. We investigated the effect of serum from @entity0 surgery @entity1 who received different anaesthetic techniques on @entity0 cell function in vitro. METHODS: @entity1 were randomized to receive @entity1875 /paravertebral anaesthesia- @entity1337 ( @entity1875 /paravertebral, n=11) or @entity2614 general anaesthesia with opioid @entity1337 ( @entity2614 /opioid, n=11). The ER-negative MDA-MB-231 cell line was treated with patient serum from both groups. The effects on proliferation and migration were measured. RESULTS: Treatment groups were well balanced for age, weight, surgical procedure, and @entity5 pathology. @entity158 scores were lower at 1 and 2 h in the @entity1875 /paravertebral @entity1337 group. Compared with preoperative values, proliferation of MDA-MB-231 cells treated with postoperative patient serum at 10% concentration from the @entity1875 /paravertebral group was significantly reduced compared with the @entity2614 /opioid group (-24% vs 73%, P=0.01). There was no significant change in MDA-MB-231 cell migration after treatment with patient serum between the two groups. CONCLUSIONS: Serum from @entity1 receiving @entity1875 /paravertebral anaesthesia for @entity0 surgery inhibited proliferation, but not migration, of ER-MDA-MB-231 cells in vitro, to a greater extent than that from @entity1 receiving @entity2614 /opioid anaesthesia- @entity1337 . This implies that anaesthetic technique alters the serum molecular milieu in ways that may affect @entity0 cell function, possibly by altering anaesthetic and opioid drug administration and resultant @entity158 scores.

Effect of anaesthetic technique on oestrogen receptor-negative XXXX cell function in vitro.

@entity1 :: ('9606', 'Species') :: ['Patients', 'patients'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer-related deaths', 'breast cancer'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity1337 :: ('MESH:D000699', 'Disease') :: ['analgesia'] @entity1875 :: ('MESH:D015742', 'Chemical') :: ['propofol'] @entity158 :: ('MESH:D010146', 'Disease') :: ['Pain', 'pain'] @entity2614 :: ('MESH:C009250', 'Chemical') :: ['sevoflurane']
@entity0 :: (MESH:D001943,Disease) :: ['breast cancer']
