UNASSIGNED: The present study was aimed at tailoring the particle size of @entity21243 (UA) nanosuspension for improved anticancer activity. UA nanosuspensions were prepared by antisolvent precipitation using a four-stream multi-inlet vortex mixer (MIVM) under defined conditions of varying solvent composition, drug feeding concentration or stream flow rate. The resulting products were characterized for particle size and polydispersity. Two of the UA nanosuspensions with mean particle sizes of 100 and 300nm were further assessed for their in-vitro activity against MCF-7 @entity0 cells using fluorescence microscopy with @entity14939 ( @entity14939 ) staining, as well as flow cytometry with propidium (PI) staining and with double staining by @entity2822 . It was revealed that the solvent composition, drug feeding concentration and stream flow rate were critical parameters for particle size control of the UA nanosuspensions generated with the MIVM. Specifically, decreasing the UA feeding concentration or increasing the stream flow rate or @entity1068 content resulted in a reduction of particle size. Excellent reproducibility for nanosuspension production was demonstrated for the 100 and 300nm UA preparations with a deviation of not more than 5% in particle size from the mean value of three independent batches. Fluorescence microscopy and flow cytometry revealed that these two different sized UA nanosuspensions, particularly the 300nm sample, exhibited a higher anti-proliferation activity against the MCF-7 cells and afforded a larger population of these cells in both early and late apoptotic phases. In conclusion, MIVM is a robust and pragmatic tool for tailoring the particle size of the UA nanosuspension. Particle size appears to be a critical determinant of the anticancer activity of the UA nanoparticles.

Particle size tailoring of XXXX nanosuspensions for improved anticancer activity by controlled antisolvent precipitation.

@entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity2822 :: ('CHEBI:37926', 'Chemical') :: ['fluorescein isothiocyanate'] @entity21243 :: ('MESH:C005466', 'Chemical') :: ['ursolic acid'] @entity14939 :: ('MESH:C007293', 'Chemical') :: ["4',6-diamidino-2-phenylindole", 'DAPI'] @entity1068 :: ('MESH:D000431', 'Chemical') :: ['ethanol']
@entity21243 :: (MESH:C005466,Chemical) :: ['ursolic acid']
