@entity320 development and progression is thought to be the result of a multi-step accumulation of @entity74 , with loss of heterozygosity in chromosome 9p ( @entity18402 ) frequently described. In addition, chromosome 10q allelic loss has been reported, implicating the @entity5 suppressor gene @entity983 / @entity983 on 10q23.3. The @entity29243 gene at 10q24-25 is another candidate @entity5 suppressor that has only rarely been studied in @entity320 , with conflicting results. We used microdissection-based genotyping to investigate 29 @entity320 from 20 @entity1 for loss of heterozygosity in intragenic and flanking microsatellite markers for this latter gene. Concurrently, the @entity18402 gene was similarly studied using two flanking microsatellites. Fifty-four percent (15 of 28) of the informative cases showed loss of heterozygosity for one or both @entity29243 markers, as compared with 67% (16 of 24) of the informative cases for @entity18402 . @entity29243 allelic loss was seen more frequently in recurrent/ @entity3 (59%), as compared with in primary (33%) lesions. Eighty percent of the @entity5 showed loss of heterozygosity for @entity18402 , as well as 63% of recurrent/metastatic ones. We studied more than one @entity5 in eight @entity1 , with those from three @entity1 showing discordant genetic patterns. One @entity1 showed a @entity3 with allelic loss for @entity29243 that was not identified in the primary @entity320 or a local recurrence. The other two @entity1 showed clonal heterogeneity in @entity29243 at synchronous and metachronous metastatic foci. These findings support @entity29243 as a putative @entity5 suppressor gene involved in conventional @entity320 progression. Genetic heterogeneity seen in different metastases from the same primary suggests a nonlinear pattern of chromosomal damage, with the development of multiple clones within the @entity5 , each acquiring its own metastatic potential.

Loss of heterozygosity in the XXXX gene is a frequent occurrence in @entity320 .

@entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'primary tumors', 'primary tumor'] @entity29243 :: ('4601', 'Gene') :: ['MXI1'] @entity18402 :: ('6298', 'Gene') :: ['MTS1'] @entity983 :: ('5728', 'Gene') :: ['MMAC1', 'PTEN'] @entity74 :: ('MESH:D030342', 'Disease') :: ['genetic damage'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastatic tumors', 'metastatic tumor'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma', 'melanomas', 'Melanoma']
@entity29243 :: (4601,Gene) :: ['MXI1']
