BACKGROUND/AIMS: Standard chemotherapy approaches for @entity99 are effective in only 20-30% of cases. The vast majority of @entity1 treated with chemotherapy experience little or no benefit with considerable @entity137 . In an effort to identify specific agents potentially effective in individual cases, 22 individuals with a @entity157 were biopsied and their @entity5 cells were grown in culture such that the expanded @entity5 cell population could be assessed for chemosensitivity to 14 different commonly used chemotherapeutic agents. METHODOLOGY: Each agent was tested in 6 wells at 6 different doses scanning a range of concentrations known to occur in vivo corresponding to subpharmacologic levels to suprapharmacologic levels. The in vitro response of each @entity5 for each agent was determined on a 0- to 5-point scale where 0 equals not sensitive and 5 equals extremely sensitive. Each of the @entity5 was successfully grown and expanded in culture over a 30- to 40-day period. Three individuals had their @entity5 biopsied and tested twice at 2 different time points. In addition, 2 liver specimens determined to be non-neoplastic liver specimens after removal were expanded in culture as controls to determine the sensitivity of normal non-neoplastic cells to the same 14 chemotherapeutic agents. RESULTS: Assuming that @entity5 would be clinically sensitive to an agent if their in vitro sensitivity score is 3 or above, then only a minority of @entity5 could be identified as chemosensitive to the 14 agents tested. Moreover, of the 2 agents ( @entity632 and @entity900 ) used most often clinically, only 14 of the 22 @entity5 were sensitive to either of these agents. Only 8 of the @entity5 demonstrated an in vitro sensitivity score of 5 for either of these 2 agents. Specifically, only 11 of the 22 @entity5 were sensitive to @entity632 and only 9 were sensitive to @entity900 . In contrast, 14 of the @entity5 showed in vitro sensitivity to 1 or more of the other 12 agents utilized for chemosensitivity determinations. CONCLUSIONS: These data demonstrate that @entity99 can be 1) grown in vitro and 2) tested for in vitro chemosensitivity. These data suggest that this methodology may identify @entity5 that are sensitive to specific chemotherapeutic agents and assist in the determination of which agents might be most useful in a given case. Selecting a chemotherapeutic agent for use using this methodology, should improve efficacy while @entity137 would be minimized.

Chemosensitivity of primary XXXX : a potential new approach to the treatment of @entity157 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity632 :: ('MESH:D004317', 'Chemical') :: ['doxorubicin'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity900 :: ('MESH:D002945', 'Chemical') :: ['cisplatin'] @entity157 :: ('MESH:D006528', 'Disease') :: ['hepatoma'] @entity99 :: ('MESH:D056486', 'Disease') :: ['hepatic tumors', 'hepatic neoplasms']
@entity99 :: (MESH:D056486,Disease) :: ['hepatic neoplasms']
