@entity421 is a primary @entity79 that typically occurs during adolescence but also has a second incidence peak in the elderly. The etiology of @entity421 is not well understood. Recent investigations have identified SV40 DNA sequences in @entity421 , suggesting that SV40 may contribute to @entity5 development. However, these studies also demonstrated geographical differences in SV40-positive @entity421 . The purpose of this study was to determine the prevalence and clinicopathological characteristics of SV40 positive @entity421 in Tunisian @entity1 . Fifty-six @entity406 -fixed paraffin-embedded specimens of @entity421 were retrospectively investigated. Samples investigated were clinical cases examined between 1990 and 2004 in the Laboratory of Pathology at the University Hospital Farhat-Hached of Sousse (Tunisia). The search for SV40 was performed by immunohistochemistry using the Pab108 antibody for the detection of the viral oncoproteins: large T antigen and small t antigen (T/t-ag). SV40 status was correlated with clinico-pathological data. T/t-ag immunostaning was detected in the @entity5 cells in 31/56 (55.4%) @entity421 cases. SV40 positivity was more frequent (83%) in @entity1 older than 40 years (5/6 cases) than in @entity1 under 40 years (52%, 26/50), but the difference does not reach statistical significance (p = 0.33). Moreover, the time between the onset of clinical symptoms and diagnosis was shorter for SV40 positive than SV40 negative cases (p = 0.08). However, the viral status did not differ significantly according to gender, @entity5 size, histological subtype, @entity5 location, or @entity3 . This study documents the presence SV40 T/t-antigens in a proportion of @entity421 in Tunisian @entity1 . The expression of these viral oncoproteins supports the hypothesis that SV40 may have a role in the pathogenesis of this @entity5 .

Immunodetection of SV40 T/t-antigens in XXXX osteosrcoma in a series of Tunisian @entity1 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity406 :: ('MESH:D005557', 'Chemical') :: ['formalin'] @entity79 :: ('MESH:D001847', 'Disease') :: ['bone malignancy'] @entity421 :: ('MESH:D012516', 'Disease') :: ['osteosarcomas', 'Osteosarcoma', 'osteosarcoma'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastases']
@entity1 :: (9606,Species) :: ['human']
