Microscopy and PCR were compared for use in the diagnosis of @entity234 ( @entity234 ) in 63 @entity1 . Aspirates of lymph nodes (samples from 52 @entity1 ), skin (23 samples), and bone marrow (18 samples) were used. For 11 @entity1 lymph node aspiration could be repeated 6 months after they recovered from @entity234 . During active @entity234 , PCR was positive for 42 of 52 (80.8%) lymph node aspirates and 19 of 23 (82.7%) skin aspirates, whereas microscopy was positive for only 9 of 52 (17.3%) lymph node aspirates and 7 of 23 (30.4%) skin aspirates. PCR was always positive when @entity4801 were seen by microscopy. When the results obtained with lymph node and skin aspirates from the same @entity1 (n = 16) were compared, there was complete agreement. Bone marrow samples were negative by microscopy and PCR for 16 @entity1 and positive by both methods for 1 @entity1 ; for one sample only the PCR was positive. PCR confirmed the co-occurrence of @entity234 and @entity234 in one @entity1 and confirmed the suspicion of this co-occurrence in the other @entity1 . After recovery, no @entity4801 were found by microscopy, but 2 of 11 (18.2%) samples were still positive by PCR. Thirty negative controls were all found to be PCR negative, and 15 positive controls were all PCR positive. Cross-reactions with @entity4430 could be ruled out. In conclusion, PCR with inguinal lymph node or skin aspirates is suitable for confirming the clinical diagnosis of @entity234 . In some @entity1 , lymph node aspirates are probably preferred because aspiration of material from the skin may leave scars.

Use of PCR for diagnosis of XXXX .

@entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity234 :: ('MESH:D007898', 'Disease') :: ['post-kala-azar dermal leishmaniasis', 'visceral leishmaniasis', 'PKDL'] @entity4801 :: ('MESH:D010272', 'Disease') :: ['parasites'] @entity4430 :: ('1769', 'Species') :: ['Mycobacterium leprae']
@entity234 :: (MESH:D007898,Disease) :: ['post-kala-azar dermal leishmaniasis']
