UNASSIGNED: Nucleotide excision repair (NER) protects against sunlight-induced @entity1489 . Defective NER is associated with photosensitivity and a high @entity1489 incidence. Some clinical treatments that cause photosensitivity can also increase @entity1489 risk. Among these, the immunosuppressant @entity500 and the @entity3284 antibiotics @entity2177 and @entity800 , interact with UVA radiation to generate reactive @entity26 species (ROS) that diminish NER capacity by causing @entity16 . The RPA @entity29086 plays a pivotal role in DNA metabolism and is an essential component of NER. The relationship between protein oxidation and NER inhibition was investigated in cultured @entity1 cells expressing different levels of RPA. We show here that RPA is limiting for NER and that oxidative damage to RPA compromises NER capability. Our findings reveal that cellular RPA is surprisingly vulnerable to oxidation and we identify oxidized forms of RPA that are associated with impaired NER. The vulnerability of NER to inhibition by oxidation provides a connection between cutaneous photosensitivity, @entity16 and @entity1489 risk. Our findings emphasize that damage to DNA repair proteins, as well as to DNA itself is likely to be an important contributor to @entity1489 risk.Journal of Investigative Dermatology accepted article preview online, 02 July 2015. doi:10.1038/jid.2015.255.

Oxidative Damage to RPA Limits the Nucleotide Excision Repair Capacity of XXXX Cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity500 :: ('MESH:D001379', 'Chemical') :: ['azathioprine'] @entity3284 :: ('D024841', 'Chemical') :: ['fluoroquinolone'] @entity1489 :: ('MESH:D012878', 'Disease') :: ['increased skin cancer', 'skin cancer'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity16 :: ('MESH:D009422', 'Disease') :: ['protein damage'] @entity2177 :: ('MESH:D002939', 'Chemical') :: ['ciprofloxacin'] @entity29086 :: ('284390', 'Gene') :: ['DNA binding protein'] @entity800 :: ('MESH:D015242', 'Chemical') :: ['ofloxacin']
@entity1 :: (9606,Species) :: ['Human']
