AIMS: Distinguishing between @entity420 of endocervical and endometrial origin histologically can be difficult, particularly in small biopsies. Most endocervical @entity420 contain @entity1082 ) deoxyribonucleic acid (DNA) of 'high-risk' (HR) types, whereas this has not been consistently demonstrated in @entity1204 . The aim of this study was to determine whether HPV DNA testing could aid in this differential diagnosis. METHODS: The frequency of HPV DNA in @entity982 -embedded tissue samples from 50 endocervical and 50 @entity1204 was investigated using polymerase chain reaction (PCR) amplification techniques involving (i) a screening HPV test followed by HPV DNA sequencing, and (ii) a test designed to detect HR genotypes 16, 18, 31, 33, 35, 45 and 58. Control specimens included @entity3146 , @entity957 ( @entity957 ) of the cervix and lung, and @entity694 . Measures to minimise cross-contamination were implemented. RESULTS: The screening test followed by HPV DNA sequencing had the highest sensitivity. By this test HR HPV DNA was detected in 11 of 11 (100%) @entity3146 ( @entity3146 ) lesions, nine of 10 (90%) cervical @entity957 , none of 10 (0%) @entity14 and none of 10 (0%) @entity957 of the lung. Thirty-nine (78%) endocervical @entity420 contained HR HPV DNA, compared to one (2.0%) @entity1204 . CONCLUSIONS: The results suggest that HPV DNA testing could be a useful adjunct in distinguishing between endocervical and @entity1204 in curettings or small biopsy specimens.

The value of HPV DNA typing in the distinction between XXXX of endocervical and endometrial origin.

@entity1204 :: ('MESH:D016889', 'Disease') :: ['endometrial adenocarcinoma', 'endometrial adenocarcinomas'] @entity957 :: ('MESH:D002294', 'Disease') :: ['SCCs', 'squamous cell carcinomas'] @entity982 :: ('MESH:D010232', 'Chemical') :: ['paraffin'] @entity1082 :: ('10566', 'Species') :: ['human papillomavirus (HPV'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal adenocarcinomas'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colonic adenocarcinomas'] @entity420 :: ('MESH:D000230', 'Disease') :: ['adenocarcinomas'] @entity3146 :: ('MESH:D018290', 'Disease') :: ['cervical intraepithelial neoplasia', 'cervical intraepithelial neoplasia (CIN) III lesions', 'CIN III']
@entity420 :: (MESH:D000230,Disease) :: ['adenocarcinoma']
