BACKGROUND: The composition of the matrix molecules is important in in vitro cell culture experiments of e.g. @entity1 @entity5 invasion and vessel formation. Currently, the @entity19 Engelbreth-Holm-Swarm (EHS) sarcoma -derived products, such as Matrigel  , are the most commonly used @entity5 ( @entity5 ) mimicking matrices for experimental studies. However, since Matrigel   is non- @entity1 in origin, its molecular composition does not accurately simulate @entity1 @entity5 . We have previously described a solid 3D organotypic @entity1868 disc invasion assay, which is derived from @entity1 uterus benign @entity5 . Here, we describe the preparation and analyses of a processed, gelatinous @entity2909 matrix, named Myogel. METHODS: A total protein extract, Myogel, was formulated from @entity1868 . The protein contents of Myogel were characterized and its composition and properties compared with a commercial @entity19 Matrigel  . Myogel was tested and compared to Matrigel   in @entity1 cell adhesion, migration, invasion, colony formation, spheroid culture and vessel formation experiments, as well as in a 3D hanging drop video image analysis. RESULTS: We demonstrated that only 34  % of Myogel's molecular content was similar to Matrigel  . All test results showed that Myogel was comparable with Matrigel  , and when mixed with low-melting agarose (Myogel-LMA) it was superior to Matrigel   in in vitro Transwell   invasion and capillary formation assays. CONCLUSIONS: In conclusion, we have developed a novel Myogel @entity5 matrix, which is recommended for in vitro @entity1 cell culture experiments since it closely mimics the @entity1 @entity5 of solid @entity5 .

A novel XXXX @entity2909 tissue derived matrix for cell culture studies.

@entity1 :: ('9606', 'Species') :: ['human'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity5 :: ('MESH:D009369', 'Disease') :: ['leiomyoma tumor', 'cancers', 'cancer', 'TME', 'tumor microenvironment'] @entity1868 :: ('MESH:D009214', 'Disease') :: ['myoma'] @entity2909 :: ('MESH:D007889', 'Disease') :: ['leiomyoma']
@entity1 :: (9606,Species) :: ['human']
