BACKGROUND: Luminal preservation of the intestine is an attractive method to locally mitigate preservation @entity5721 in small bowel transplantation (SBT) because this method has a potential to maintain the intestinal graft integrity. Hydrogen is noted as an antioxidant material by reducing hydroxyl radicals. We hypothesized that hydrogen-containing solution can be an optimum material for luminal preservation method in SBT. METHODS: Ischemic reperfusion was induced in Lewis @entity35 by occlusion of the supramesenteric artery and vein for 90 min. Experimental protocols were divided into four groups: sham operation group, no luminal injection (control) group, luminal injection of 5% glucose saline (GS) solution group, and luminal injection of @entity13897 ( @entity13897 ) group. Two milliliters of experimental solution was locally injected into the lumen of the intestine before declamping of vessels. Oxidative stress markers, proinflammatory cytokines, apoptosis in the crypt cells, and morphologic changes of the intestine were assessed. RESULTS: The production of malondialdehyde and 8-hydroxydeoxyguanosine, as oxidative stress markers, were markedly suppressed in @entity13897 group. The level of proinflammatory cytokines, such as inducible nitric oxide synthase and @entity568 , was significantly inhibited in @entity13897 group. Crypt apoptosis was also significantly suppressed in @entity13897 group. Histopathologically, integrity of villus in intestine was maintained in @entity13897 group in comparison to the other groups. CONCLUSION: Luminal injection of hydrogen-rich solution can reduce oxidative stress and consequently ameliorate ischemic-reperfusion injury. Hydrogen-containing solution can be a novel and promising luminal preservation material in SBT.

Luminal Injection of Hydrogen-Rich Solution Attenuates @entity5721 in XXXX .

@entity35 :: ('10116', 'Species') :: ['rats'] @entity13897 :: ('MESH:D018149', 'Disease') :: ['hydrogen-rich GS', 'HRGS'] @entity568 :: ('24498(Tax:10116)', 'Gene') :: ['interleukin-6'] @entity5721 :: ('MESH:D015427', 'Disease') :: ['injury and ischemic-reperfusion injury']
@entity35 :: (10116,Species) :: ['Rats']
