Telomerase adds hexameric repeats of 5'-TTAGGG-3' termed telomeres to ends of chromosomal DNA. This enzyme has been implicated in cellular immortalization and cellular senescence. Recently, a number of relevant genes have been cloned, including these encoding three major components of @entity1 telomerase: @entity1 telomerase RNA component ( @entity7066 ), @entity1 telomerase reverse transcriptase ( @entity2215 ), and @entity11071 ( @entity11071 ). Also important are genes encoding @entity1 @entity64710 . To clarify mechanisms regulating telomerase activity, we studied telomerase activity, the telomeric restriction fragment (TRF) length and gene expression of these telomerase components and the telomeric-repeat binding factor proteins in sequential observation following X-irradiation of cultured @entity788 cells. We previously reported that PANC-1 cells are better able to tolerate thermal stress, antineoplastic drugs, and exposure to @entity5 factor than MIAPaCa-2 cells. MIAPaCa-2 and PANC-1 cells were exposed to X-irradiation, their telomerase activity was increased at 2 days and then decreased gradually. Of the three telomerase components, only @entity2215 mRNA expression showed parallel changes. TRF length was stable just before and after X-irradiation. Among binding factor proteins, @entity7071 mRNA showed reciprocal changes possibly directed toward maintaining a stable telomere length. In this study, our results demonstrate that not only @entity2215 but also @entity7071 are important regulator of telomerase activity.

Telomerase reverse transcriptase and telomeric-repeat binding factor protein 1 as regulators of telomerase activity in XXXX cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity788 :: ('MESH:D010190', 'Disease') :: ['pancreatic cancer'] @entity7066 :: ('7012', 'Gene') :: ['hTR'] @entity11071 :: ('7011', 'Gene') :: ['telomerase-associated protein-1', 'TEP1'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumour necrosis'] @entity64710 :: ('7013;7014', 'Gene') :: ['telomeric-repeat binding factor protein (TRF) 1 and 2'] @entity2215 :: ('7015', 'Gene') :: ['hTERT'] @entity7071 :: ('7013', 'Gene') :: ['TRF1']
@entity788 :: (MESH:D010190,Disease) :: ['pancreatic cancer']
