PURPOSE: @entity957 ( @entity957 ) is commonly preceded by oral potentially malignant lesions (OPML). The aim of the present study was to assess, by @entity9736 next-generation sequencing (NGS), the methylation status of a list of candidate genes obtained from oral brushings to early detect OPML and @entity957 . MATERIAL AND METHODS: Oral brushing specimens from 11 @entity957 , 11 high-grade squamous intraepithelial lesions (HG- @entity13763 ), 9 low-grade @entity13763 (LG- @entity13763 ), 9 oral lichen planus (OLP), and 8 healthy donors were included in this study. We investigated, by means of @entity9736 NGS, the promoter of @entity31088 , @entity6755 , @entity19572 , @entity986 [ @entity986 ], @entity5962 , @entity21069 , and @entity26261 . Statistical significance between lesions and a pool of healthy donors were evaluated with the Mann-Whitney U test. RESULTS: @entity6755 was found to be hypermethylated in 100% of @entity957 and HG- @entity13763 and in 28.6% of LG- @entity13763 . @entity31088 hypomethylation was detected in 90.9% @entity957 and HG- @entity13763 and in 37.5% of LG- @entity13763 . @entity21069 was hypermethylated in 100% of OLP, 44.4% of @entity957 , 40% HG- @entity13763 , and 25% LG- @entity13763 . @entity19572 hypermethylation was found to be associated with @entity876 mutations (p  <  0.0001). CONCLUSION: In the present preliminary cohort of @entity1 , DNA methylation analysis of @entity31088 and @entity6755 seems to be a promising noninvasive tool for early detection of @entity957 and HG @entity13763 from oral brushing specimens.

DNA methylation analysis by @entity9736 next-generation sequencing for early detection of XXXX and high-grade squamous intraepithelial lesion from oral brushing.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity986 :: ('1029', 'Gene') :: ['CDKN2A', 'p16'] @entity26261 :: ('494324', 'Gene') :: ['miR375'] @entity957 :: ('MESH:D002294', 'Disease') :: ['OSCC', 'Oral squamous cell carcinoma'] @entity19572 :: ('547', 'Gene') :: ['KIF1A'] @entity13763 :: ('6491', 'Gene') :: ['SIL'] @entity5962 :: ('999', 'Gene') :: ['CDH1'] @entity9736 :: ('MESH:C042345', 'Chemical') :: ['bisulfite'] @entity21069 :: ('406928', 'Gene') :: ['miR137', 'MiR137'] @entity31088 :: ('2812', 'Gene') :: ['GP1BB'] @entity876 :: ('7157', 'Gene') :: ['TP53'] @entity6755 :: ('7535', 'Gene') :: ['ZAP70']
@entity957 :: (MESH:D002294,Disease) :: ['oral squamous cell carcinoma']
