UNASSIGNED: @entity3 signature genes in @entity0 have been studied comparing transcriptomic profiles of highly metastatic @entity5 cell lines established by intra-circulation injection with that of their parental cell line. However, this method is not suitable to analyze the initial steps of @entity3 including invasion into local tissues and the circulatory system. To characterize the molecular mechanisms of early @entity3 , we established highly metastatic MDA-MB-231 cell lines that metastasized to lung by the two animal transplantation models: the orthotopic transplantation method, which mimics all steps of @entity3 , or intra-circulation injection method. We then performed data-mining and network analysis of gene expression profiles of metastatic cell lines established by each transplantation method. Transcriptome analysis of seven metastatic cell lines revealed novel lung @entity3 signature genes, including known @entity3 promoting genes and signature genes. In the OXconc (orthotopic xenograft concentration) signature, 'chemotaxis' and 'cell adhesion' terms were enriched. In the TVIconc (tail vein injection concentration) signature, 'antigen recognition' and 'cell adhesion' were enriched. Furthermore, network analysis of the @entity3 signature genes highlighted hub genes in the gene regulatory network. Our findings show that expression profiles of highly metastatic cell lines were different between the orthotopic transplantation and intra-circulation injection method. It also indicates that some metastatic signature genes have been missed in previous studies. Characterization of @entity3 genes using the orthotopic transplantation method will be helpful in understanding the multi-step mechanisms of @entity3 . Signature genes in OXconc may have the potential to become prognostic markers.

Comparative analysis of gene regulatory networks of highly metastatic XXXX cells established by orthotopic transplantation and intra-circulation injection.

@entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis', 'Metastasis']
@entity0 :: (MESH:D001943,Disease) :: ['breast cancer']
