@entity3838 derivative (HpD) is a systemically administered photosensitizing agent that may be of value in the treatment of solid @entity5 . When corneal endothelial cells were perfused in the specular microscope with HpD and exposed to a 25-W incandescent light at 5 cm (5.5 mW/cm2) there was anatomic disruption of corneal endothelial cells and swelling of the @entity2687 . Perfusion with 0.2 microliter/ml (1.0 microgram/ml) HpD and 5 min exposure to light resulted in a @entity4 of 71 +/- 4 microns after 3 hr, whereas perfusion with 0.2 microliter/ml HpD and a 1-min exposure to light resulted in a @entity4 of 36 +/- 4 microns after 3 hr. Perfusion with 0.2 microliter/ml HpD with no light exposure resulted in a @entity4 of 22 +/- 4 microns after 3 hr. Inclusion of 100 micrograms/ml @entity7525 in the perfusion solution resulted in a significant 38% reduction of the @entity4 . The inclusion of either 100 micrograms/ml @entity224 dismutase, 15 mM @entity2656 , 5 mM @entity620 , 1/4% DMSO, 50 microns EDTA, 50 microns DETAPAC, 10 mM @entity14614 , or 1 mM @entity4627 did not modify the @entity4 induced by the photosensitization reaction. Perfusion of corneal endothelial cells with 2 microliters/ml (10 micrograms/ml) HpD and exposure to 25-W incandescent light for 5 min resulted in swelling of mitochondria, the appearance of vacuoles in the cytoplasm, and rapid @entity4 . The data suggests that corneal endothelial cells can be damaged by @entity273 generated by the dismutation of @entity224 anion produced during the photoreaction. @entity224 anion itself and @entity3787 -free radical do not appear to participate in causing the endothelial cell damage. The role of singlet @entity26 remains somewhat unclear. The data suggests that further in vivo studies should be performed to delineate precautions that should be taken to protect the corneal endothelium during photoradiation therapy.

Effect of XXXX derivative on rabbit corneal endothelial cell function and ultrastructure.

@entity2656 :: ('MESH:D008353', 'Chemical') :: ['D-mannitol'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumors'] @entity4 :: ('MESH:D004487', 'Disease') :: ['corneal swelling'] @entity620 :: ('MESH:D001205', 'Chemical') :: ['ascorbic acid'] @entity4627 :: ('MESH:D019810', 'Chemical') :: ['sodium azide'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity3838 :: ('MESH:D006415', 'Chemical') :: ['Hematoporphyrin'] @entity14614 :: ('CHEBI:15971', 'Chemical') :: ['L-histidine'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['hydrogen peroxide'] @entity3787 :: ('MESH:D017665', 'Chemical') :: ['hydroxyl'] @entity2687 :: ('MESH:D003316', 'Disease') :: ['corneal stroma'] @entity224 :: ('MESH:D013481', 'Chemical') :: ['Superoxide', 'superoxide'] @entity7525 :: ('100340891(Tax:9986)', 'Gene') :: ['catalase']
@entity3838 :: (MESH:D006415,Chemical) :: ['hematoporphyrin']
