The present study was aimed to investigate how the induced pluripotent stem cells (iPSCs) and bone marrow mesenchymal stem cells (BMSCs) differentiate into neuron-like cells under the induction of hippocampal microenvironments and @entity42904 's regulation. iPSCs or BMSCs were co-cultured with @entity164 ( @entity164 ) or genotypic hippocampal slice and cerebral homogenate supernatant, then the stem cells' differentiation under the induction of hippocampal environment was observed by using immunofluorescence technique. In the meantime, stem cells were co-cultured with hippocampal slice and cerebral conditioned medium of reeler ( @entity42904 deletion) @entity19 respectively. The results showed that both adhesive iPSCs and BMSCs on @entity164 hippocampal slice exhibited lamination of double "C" shape with high density on granular and @entity655 . The stem cells could differentiate into neuron-like cells with obvious polarization on @entity164 hippocampal slice. In pyramidal cell layer, the differentiated neuron-like cells were oriented vertically with similar shapes of pyramidal cell in vivo, and the cells within molecule layer were arranged horizontally. In addition, adhesive iPSCs and BMSCs could differentiate into Nestin positive neural stem cells and @entity7079 positive neurons, respectively, under @entity164 hippocampal microenvironment. On the other hand, under induction of hippocampal microenvironment of reeler @entity19 , iPSCs and BMSCs differentiation could also be seen, but their lamination was in disorder, and cell polarization was irregular. Moreover, differentiation and polarization of the iPSCs and BMSCs were delayed. These results suggest both iPSCs and BMSCs can differentiate into neuron-like cells under the induction of hippocampal microenvironments. @entity42904 is involved in the regulation of neuronal differentiation and cell polarization. Without @entity42904 , the cellular lamination and polarization appear irregular, and the stem cells' differentiation is delayed.

[Microenvironments induce iPSCs and BMSCs into neuron-like cells-- XXXX 's regulative role in cell differentiation and polarization].

@entity19 :: ('10090', 'Species') :: ['mouse'] @entity164 :: ('MESH:D017827', 'Disease') :: ['WT', 'wild type'] @entity655 :: ('MESH:C535504', 'Disease') :: ['pyramidal layers'] @entity7079 :: ('52897(Tax:10090)', 'Gene') :: ['NeuN'] @entity42904 :: ('19699(Tax:10090)', 'Gene') :: ['Reelin']
@entity42904 :: (19699(Tax:10090),Gene) :: ['Reelin']
