Cadmium is a widely distributed industrial and environmental toxin. The principal target organ of chronic sublethal cadmium exposure is the kidney. In renal epithelial cells, acute high-dose cadmium exposure induces differential expression of proteins, including @entity1457 proteins. However, few studies have examined @entity1457 protein expression in cells after prolonged exposure to cadmium at sublethal concentrations. Here, we assayed total cell protein, neutral red uptake, cell death, and levels of @entity69519 and @entity1457 proteins @entity25485 and inducible @entity21363 in cultures of MDCK and LLC-PK1 renal epithelial cells treated with cadmium for 3 days. Treatment with cadmium at concentrations equal to or greater than 10 microM (LLC-PK1) or 25 microM (MDCK) reduced measures of cell vitality and induced cell death. However, a concentration-dependent increase in @entity25485 was detected in both cell types treated with as little as 5 microM cadmium. Accumulation of @entity21363 was correlated only with cadmium treatment at concentrations also causing cell death. @entity69519 was maximally detected in cells treated with cadmium at concentrations that did not reduce cell vitality, and further increases were not detected at greater concentrations. These results reveal that @entity1457 proteins accumulate in renal epithelial cells during prolonged cadmium exposure, that cadmium induces differential expression of @entity1457 protein in epithelial cells, and that protein expression patterns in epithelial cells are specific to the cadmium concentration and degree of cellular injury. A potential role for @entity25485 in the cellular response to sublethal cadmium-induced injury is also implicated by our results.

XXXX , @entity21363 , and @entity69519 in MDCK and LLC-PK1 renal epithelial cells: effects of prolonged exposure to cadmium.

@entity69519 :: ('100855992', 'Gene') :: ['Metallothionein', 'metallothionein'] @entity1457 :: ('MESH:D012769', 'Disease') :: ['heat shock'] @entity21363 :: ('403612(Tax:9615)', 'Gene') :: ['Hsp70'] @entity25485 :: ('403979(Tax:9615)', 'Gene') :: ['Hsp27']
@entity25485 :: (403979(Tax:9615),Gene) :: ['Hsp27']
