BACKGROUND: The effects of simulated " @entity63 " and "reperfusion" were evaluated in cell cultures of @entity1 ventricular cardiomyocytes and @entity1 saphenous vein endothelial cells. METHODS: Myocyte and endothelial cell cultures were exposed to a low volume (1.5 ml) of either @entity519 ( @entity26 tension = 16 mm Hg) or anoxic ( @entity26 tension = 0 mm Hg) @entity1391 -buffered saline solution for 90 minutes (" @entity63 ") followed by 30 minutes of simulated "reperfusion." @entity131 was evaluated by @entity3460 exclusion. Next, the effects of a preconditioning stimulus were evaluated by a brief (10 minute) exposure to @entity519 or @entity63 and 10 minutes of reperfusion before prolonged (90 minutes) @entity63 . Finally, the effects of anoxic preconditioning on intracellular @entity880 accumulation and extracellular @entity880 and acid release were assessed. RESULTS: " @entity63 " and "reperfusion" resulted in greater injury to endothelial cells than to cardiomyocytes. In both cell types, @entity63 resulted in greater injury than @entity63 . Preconditioning reduced cell injury in myocytes but not in endothelial cells. Endothelial cells produced more @entity880 than cardiomyocytes under normoxic conditions. @entity63 increased @entity880 accumulation and release in cardiomyocytes but not endothelial cells. Preconditioning reduced @entity880 accumulation and release in cardiomyocytes but not endothelial cells. CONCLUSIONS: Endothelial cells were more susceptible to the same period of simulated @entity63 than cardiomyocytes. Preconditioning protected cardiomyocytes but not endothelial cells from a subsequent prolonged period of @entity63 and reperfusion.

Preconditioning XXXX cardiomyocytes and endothelial cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity131 :: ('MESH:D001930', 'Disease') :: ['Cell injury'] @entity63 :: ('MESH:D007511', 'Disease') :: ['Ischemia', 'anoxic ischemia', 'ischemia', 'hypoxic ischemia'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity519 :: ('MESH:D000860', 'Disease') :: ['hypoxic'] @entity1391 :: ('CHEBI:26020', 'Chemical') :: ['phosphate'] @entity3460 :: ('MESH:D014343', 'Chemical') :: ['trypan blue'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate']
@entity1 :: (9606,Species) :: ['human']
