The aim of the present study was to determine the composition of @entity1522 that were truly digestible in the ileum. Several @entity291 milk products and two soy protein products were tested using the newly developed enzymatically hydrolyzed casein-ultrafiltration (UF) method. This method provides a novel approach for determining endogenous flows of @entity1522 at the terminal ileum, which are required for correcting apparent ileal digestibility values to true digestibility values. Digestibility was determined by sampling digesta of Sprague-Dawley male @entity35 at the end of the small intestine (ileum). @entity6213 was used as an indigestible marker. The traditional protein-free method for determining endogenous losses of @entity1522 was also used for comparison with the enzymatically hydrolyzed casein-UF method. Flows of endogenous @entity1522 at the terminal ileum of the @entity35 following peptide alimentation were generally higher (1.8-fold) than those determined after a protein-free diet was fed. Compared with values for true @entity535 digestibility, apparent values underestimated digestibility by 2 to 30%. True @entity535 digestibility was high (79 to 102%) for all of the protein sources. The digestible amounts of @entity1853 and @entity376 were 2 and 1.3 times higher, respectively, in dairy proteins than in soy proteins. The enzymatically hydrolyzed casein-UF method provides a physiological estimate of endogenous @entity535 flow and appeared to be an appropriate method for correcting apparent digestibility values to true digestibility values. The data for true ileal digestibility of @entity1522 obtained using this technique demonstrated the high quality of @entity291 milk proteins.

The digestible XXXX composition of several milk proteins: application of a new bioassay.

@entity6213 :: ('MESH:C023600', 'Chemical') :: ['Chromic oxide'] @entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity1853 :: ('MESH:D008715', 'Chemical') :: ['methionine'] @entity376 :: ('CHEBI:25094', 'Chemical') :: ['lysine'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity291 :: ('9913', 'Species') :: ['bovine']
@entity535 :: (CHEBI:33704,Chemical) :: ['amino acid']
