Although it is well documented that soluble beta amyloid (Ab) oligomers are critical factors in the pathogenesis of @entity195 ( @entity195 ) by causing synaptic dysfunction and @entity528 , the primary mechanisms by which Ab oligomers trigger neurodegeneration are not entirely understood. We sought to investigate whether toxic small Ab1-42 oligomers induce changes in plasma membrane potential of cultured neurons and glial cells in @entity35 cerebellar granule cell cultures leading to @entity528 and whether these effects are sensitive to the @entity1024 receptor ( @entity143 -R) antagonist @entity3390 . We found that small Ab1-42 oligomers induced rapid, protracted membrane depolarization of both neurons and microglia, whereas there was no change in membrane potential of astrocytes. @entity3390 did not modulate Ab-induced @entity528 . In contrast, Ab1-42 oligomer-induced decrease in plasma membrane potential of microglia was prevented by @entity3390 . Small Ab1-42 oligomers significantly elevated extracellular @entity2227 and caused neuronal @entity39 , and both were prevented by @entity3390 . Also, small Ab1-42 oligomers decreased resistance of isolated brain mitochondria to @entity460 -induced opening of mitochondrial permeability transition pore. In conclusion, the results suggest that the primary effect of toxic small Ab oligomers on neurons is rapid, @entity143 -R-independent plasma membrane depolarization, which leads to @entity528 . Ab oligomers-induced depolarization of microglial cells is @entity143 -R dependent.    2014 Wiley Periodicals, Inc.

Small Ab1-42 oligomer-induced membrane depolarization of neuronal and microglial cells: Role of XXXX receptors.

@entity1024 :: ('CHEBI:31882', 'Chemical') :: ['N-methyl-D-aspartate'] @entity2227 :: ('CHEBI:29987', 'Chemical') :: ['glutamate'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis'] @entity143 :: ('CHEBI:6121', 'Chemical') :: ['NMDA'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity195 :: ('MESH:D000544', 'Disease') :: ['AD', "Alzheimer's disease"] @entity35 :: ('10116', 'Species') :: ['rat'] @entity3390 :: ('MESH:D016291', 'Chemical') :: ['MK801'] @entity528 :: ('MESH:D009410', 'Disease') :: ['neuronal depolarization', 'neuronal death']
@entity1024 :: (CHEBI:31882,Chemical) :: ['N-methyl-D-aspartate']
