1. The effects of @entity49438 ( @entity31471 ) on dissociated @entity35 dorsal raphe neurones were investigated using the patch-clamp method. 2. Under current-clamp conditions, @entity31471 hyperpolarized the cell membrane, resulting in the reduction of firing rates. 3. Under voltage-clamp conditions, @entity31471 induced an inward rectifying K+ current in a concentration-dependent manner. 4. The @entity31471 -induced K+ currents (I( @entity31471 )) were mimicked by @entity10097 , a @entity10463 agonist. The I( @entity31471 ) was blocked by @entity11054 , a @entity10463 receptor antagonist, in a concentration-dependent manner. 5. @entity8816 , an agent which uncouples between the receptor and the G-protein, irreversibly blocked the I( @entity31471 ). 6. In neurones perfused intracellularly with a pipette-solution containing @entity3007 using the conventional whole-cell patch recording, the I( @entity31471 ) showed a gradual rundown. When the neurones were perfused with GTPgammaS, @entity31471 activated the inwardly rectifying K+ current in an irreversible manner. 7. In the inside-out patch recording mode, @entity31471 -activated single K+ channel currents (i( @entity31471 )) which also showed an inward rectification. When the @entity3006 in cytosolic side was completely replaced with @entity3007 , the open probability of i( @entity31471 ) significantly increased. 8. These results indicate that the activation of @entity10463 receptors by @entity31471 directly opens the inward rectifying K+ channels via a G-protein mediated process.

@entity49438 -induced K+ current in acutely dissociated XXXX dorsal raphe neurones.

@entity3007 :: ('MESH:D006160', 'Chemical') :: ['GTP'] @entity31471 :: ('MESH:C076628', 'Chemical') :: ['TDS'] @entity10097 :: ('MESH:D017371', 'Chemical') :: ['8-OH-DPAT'] @entity11054 :: ('MESH:D013134', 'Chemical') :: ['spiperone'] @entity10463 :: ('24473(Tax:10116)', 'Gene') :: ['5-HT1A'] @entity8816 :: ('MESH:D005033', 'Chemical') :: ['N-Ethylmaleimide'] @entity3006 :: ('MESH:D006153', 'Chemical') :: ['GDP'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity49438 :: ('MESH:C055267', 'Chemical') :: ['tandospirone']
@entity35 :: (10116,Species) :: ['rat']
