A method is presented in this report for concurrent analysis of @entity455 , blood perfusion and proliferation characteristics in @entity5 cross-sections of @entity1 @entity189 and @entity463 xenografts. @entity5 were implanted subcutaneously in @entity19 . After i.v. injection with @entity5956 and @entity1603 (BrdUrd) as perfusion and proliferation markers, animals were killed. An antiendothelial antibody (9F1) was used to delineate vascular structures. Cross-sections were analysed by a multistep immune staining and a computer-controlled microscope scanning method. Each @entity5 section was stained and scanned four times (Hoechst, 9F1, BrdUrd and Fast Blue for all nuclei). When these images were combined, vasculature, perfusion and proliferation parameters were analysed. The @entity894 ( @entity894 ) was defined as the ratio of the BrdUrd-labelled area to the total nuclear area. The @entity894 based on manual counting and the @entity894 calculated by flow cytometry (FCM) were in good agreement with the @entity894 based on surface analysis. @entity894 decreased at increasing distance from its nearest vessel. In the vicinity of perfused vessels, the @entity894 was 30-70% higher than near non-perfused vessels. This method shows that both vasculature/perfusion and proliferation characteristics can be measured in the same @entity5 section in a semiautomatic way. This could be applied in clinical practice to identify combined @entity1 @entity5 characteristics that predict for a favourable response to treatment modifications.

Multiparameter analysis of vasculature, perfusion and proliferation in @entity1 XXXX xenografts.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1603 :: ('MESH:D001973', 'Chemical') :: ['bromodeoxyuridine'] @entity455 :: ('MESH:D014652', 'Disease') :: ['vascular architecture'] @entity5956 :: ('MESH:C017807', 'Chemical') :: ['Hoechst 33342'] @entity894 :: ('MESH:C566784', 'Disease') :: ['labelling index', 'LI'] @entity5 :: ('MESH:D009369', 'Disease') :: ['Tumours', 'tumour', 'whole-tumour'] @entity19 :: ('10090', 'Species') :: ['nude mice'] @entity189 :: ('MESH:D002277', 'Disease') :: ['larynx carcinoma'] @entity463 :: ('MESH:D005909', 'Disease') :: ['glioblastoma']
@entity5 :: (MESH:D009369,Disease) :: ['tumour']
