UNASSIGNED: Tryptase exacerbates @entity63 -reperfusion injury, however, the direct role of tryptase in @entity3137 and the underlying mechanism remains largely unknown. Protease-activated receptor  2 ( @entity18515 ), commonly activated by tryptase, interacts with various adaptor proteins, including b  -arrestin  -2. The present study aimed to determine whether tryptase is capable of inducing @entity1944 via @entity18515 activation and to define the role of b  -arrestin  -2 in the process of injury. The IEC  -6 @entity35 intestinal epithelial cell line was challenged by tryptase stimulation. Cell viability, @entity880 dehydrogenase (LDH) activity and apoptosis were analyzed to determine the severity of cell injury. Injury was also evaluated following treatments with specific @entity18515 and extracellular signal  -related kinases ( @entity2509 ) inhibitors, and knockdown of b  -arrestin  -2. @entity18515 , @entity2509  -arrestin  -2 protein expression levels were evaluated. Tryptase treatment (100  and  1,000  ng/ml) resulted in IEC  -6 cell injury, as demonstrated by significant reductions in cell viability, accompanied by concomitant increases in LDH activity and levels of cleaved caspase  -3 protein expression. Furthermore, tryptase treatment led to a marked increase in @entity18515 and phosphorylated  - @entity2509 expression, and exposure to specific @entity18515 and @entity2509 inhibitors eliminated the changes induced by tryptase. Knockdown of b  -arrestin  -2 blocked tryptase  -mediated cell injury, whereas tryptase exerted no influence on b  -arrestin  -2 expression in IEC  -6 cells. These data indicate that tryptase may directly damage IEC  -6 cells via @entity18515 and the downstream activation of @entity2509 , and demonstrate that the signaling pathway requires b-arrestin-2.

XXXX induced by tryptase-activated protease-activated receptor  2 requires b-arrestin-2 in  vitro.

@entity1944 :: ('MESH:D007410', 'Disease') :: ['intestinal mucosal cell injury'] @entity63 :: ('MESH:D007511', 'Disease') :: ['intestinal ischemia'] @entity2509 :: ('24338(Tax:10116)', 'Gene') :: ['ERK and b', 'ERK'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity18515 :: ('116677(Tax:10116)', 'Gene') :: ['PAR  -2', 'PAR-2'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity3137 :: ('MESH:D009059', 'Disease') :: ['intestinal mucosal injury']
@entity3137 :: (MESH:D009059,Disease) :: ['Intestinal mucosal injury']
