Granulosa cells (GCs) were characterised morphologically by light and electron microscopy. The steroidogenic capability of GCs in vitro was estimated by radioimmunoassay (RIA): @entity53 (E2), @entity1420 (P) and @entity2671 (A) secreted into the culture medium were measured. The influence of several culture media and anchorage of the cell either to plastic vessels (monolayer) or to collagen fibrils (in gel) were studied. As the various culture media were assayed with regard to their suitability for IVF, it was found that Ham's F10 is quite satisfactory (in agreement with other observations on embryo cultures). A chemically defined medium BM 86 was found to be inadequate. In addition to the two cell types which are known, a third cell type which can perform efficient aromatisation (E2 production) in vitro is characterised here. The influence of @entity851 ( @entity851 ) like insulin-like @entity851 ( @entity442 ), epidermal @entity851 (EGF), platelet-derived @entity851 (PDGF) and fibroblast @entity851 (FGF) on steroidogenesis was tested either alone or with @entity1 chorionic gonadotrophin ( @entity3566 ). Except for @entity53 (E2) from early GCs, @entity3566 generally stimulated @entity1420 (P) and E2 secretion. EGF by itself enhanced the secretion of P but not of E2. EGF did not affect @entity3566 stimulation of P, but reduced that of E2. In contrast, in pre-ovulatory GCs @entity442 reduced the stimulatory effect of @entity3566 on both @entity10819 . In early GCs @entity442 potentiated @entity3566 stimulation of P. In early GCs, neither @entity3566 nor @entity442 nor a combination of @entity442 with @entity3566 had any effect on E2 production.

XXXX granulosa cells in vitro: characteristics of growth, morphology and influence of some cytokines on steroidogenesis.

@entity1 :: ('9606', 'Species') :: ['human'] @entity851 :: ('MESH:D006130', 'Disease') :: ['GF', 'cytokines/growth factors'] @entity1420 :: ('MESH:D011374', 'Chemical') :: ['progesterone'] @entity442 :: ('3479', 'Gene') :: ['IGF-1', 'IGF-I'] @entity53 :: ('MESH:D004958', 'Chemical') :: ['oestradiol'] @entity3566 :: ('3342', 'Gene') :: ['hCG'] @entity2671 :: ('MESH:D000735', 'Chemical') :: ['androstenedione'] @entity10819 :: ('6044', 'Gene') :: ['E2 and P']
@entity1 :: (9606,Species) :: ['Human']
