@entity1897 ( @entity1897 ) isografts provide a favourable environment for axon regeneration after peripheral and central nervous system (CNS) injury, but definitive information on the extent of cellular intermixing between donor and host tissues is lacking. We wished to compare migration patterns in fresh and predegenerate @entity1897 grafts, and also compare the extent of cell migration after transplantation to peripheral nervous system (PNS) versus CNS. To discern how host and donor cells interact after @entity1897 transplantation, sciatic nerve segments were transplanted from inbred adult @entity19 into @entity1897 ( @entity1897 ) or into lesioned cerebral cortex of opposite gender siblings. Migrating male cells were identified using a Y-chromosome-specific probe and in situ hybridization methods, and characterized immunohistochemically. The extent of donor and host cellular intermixing was similar in fresh and predegenerate @entity1897 isografts. There was substantial intermixing of donor and host cells by 8 days. Many host cells migrating into epineurial regions of grafts were immunopositive for @entity11496 (macrophages). The endoneurium of grafted @entity1897 was also colonized by host cells; some were @entity11496 + but many were immunostained with S-100 (Schwann cell marker). Donor S-100+ Schwann cells rapidly migrated out into proximal and distal host @entity1897 and by 12 weeks were found at least 2 mm from the grafts. Endoneurial microvessels in grafts were mostly donor-derived. By comparison, in male @entity1897 grafts to female CNS, even after 6 weeks few donor cells had migrated out into surrounding host cortex, despite the observation that almost all grafts contained regenerating axons and were thus attached to host CNS tissue.

Migration of cells into and out of peripheral nerve isografts in the peripheral and central nervous systems of the adult XXXX .

@entity19 :: ('10090', 'Species') :: ['mice'] @entity1897 :: ('MESH:D010523', 'Disease') :: ['PN-PN grafts', 'PN-PN', 'PN defects', 'Peripheral nerve', 'PN'] @entity11496 :: ('13733(Tax:10090)', 'Gene') :: ['F4/80']
@entity19 :: (10090,Species) :: ['mouse']
