@entity1 with @entity0 in a distinct stage of disease can benefit from @entity34 ( @entity34 ) with autologous stem cell support; however, a significant number of these @entity1 relapse despite this intensive treatment. This study investigates the persistence of @entity5 on the single-cell level. A total of 194 data sets consisting of bone marrow and blood samples obtained prior to and after @entity34 and of aliquots of apheresis products were searched with immunocytochemistry and reverse transcriptase polymerase chain reaction (RT-PCR) for disseminated @entity5 cells. Presence of @entity5 cells in the marrow is frequent prior to and after @entity34 , but @entity34 reduces the amount of malignant cells in marrow significantly. In contrast, there was no effect on the number of circulating @entity5 cells. Reinfusion of contaminated apheresis products was surprisingly associated with a low number of malignant cells in bone marrow after @entity34 and vice versa. The impact of disseminated @entity5 cells in bone marrow, apheresis, and peripheral blood on disease-free survival after @entity34 could be investigated in a total of 165 samples. Surprisingly, neither the presence of @entity5 cells in marrow or blood nor in apheresis was associated with a bad prognosis in Kaplan-Meyer survival analysis. These results suggest that apheresis products and bone marrow should be regarded as different biological compartments for @entity5 cells. It can be concluded that complete elimination of disseminated @entity5 cells by @entity34 is not always possible. The theory of reinduction of metastatic @entity0 by accidentally reinfused contaminants is not supported by this study so far. However, further research is necessary to identify distinct cell populations with the potentially capacity to metastasize.

Disseminated XXXX cells prior to and after @entity34 .

@entity1 :: ('9606', 'Species') :: ['patients', 'Women'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'epithelial cancer', 'malignancy'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity34 :: ('MESH:D016609', 'Disease') :: ['high-dose therapy', 'HDT']
@entity0 :: (MESH:D001943,Disease) :: ['breast cancer']
