UNASSIGNED: To understand the cross-talk and specificity of the early responses of plants to salt and drought, we performed physiological and proteome analyses of @entity4335 seedlings pretreated with 245 mM @entity4202 or 25% @entity968 ( @entity970 ) 6000 under identical osmotic pressure (-1.0 MPa). Significant decreases in water content and photosynthetic rate and excessive accumulation of compatible osmolytes and oxidative damage were observed in response to both stresses. Unexpectedly, the drought response was more severe than the salt response. We further identified 45 common differentially expressed proteins (DEPs), 143 salt-specific DEPs and 160 drought-specific DEPs by isobaric tags for relative and absolute quantitation (iTRAQ) analysis. The proteome quantitative data were then confirmed by multiple reaction monitoring (MRM). The differences in the proteomic profiles between drought-treated and salt-treated seedlings exceeded the similarities in the early stress responses. Signal perception and transduction, transport and membrane trafficking, and photosynthesis-related proteins were enriched as part of the molecular cross-talk and specificity mechanism in the early responses to the two abiotic stresses. The @entity141 signaling, G protein-related signaling, 14-3-3 signaling pathway and phosphorylation cascades were the common signal transduction pathways shared by both salt and drought stress responses; however, the proteins with executive functions varied. These results indicate functional specialization of family proteins in response to different stresses, i.e., CDPK21, TPR, and @entity68844 specific to phosphorylation cascades under early salt stress, whereas STN7 and BSL were specific to phosphorylation cascades under early drought stress. Only the @entity460 -binding EF-hand family protein and ZKT were clearly identified as signaling proteins that acted as cross-talk nodes for salt and drought signaling pathways. Our study provides new clues and insights for developing strategies to improve the tolerance of crops to complex, multiple environmental stresses.

Elucidation of Cross-Talk and Specificity of Early Response Mechanisms to Salt and XXXX -Simulated Drought Stresses in @entity4335 Using Comparative Proteomic Analysis.

@entity4335 :: ('3708', 'Species') :: ['Brassica napus'] @entity968 :: ('MESH:D011092', 'Chemical') :: ['polyethylene glycol'] @entity68844 :: ('106372651', 'Gene') :: ['CTR1'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+'] @entity970 :: ('CHEBI:46793', 'Chemical') :: ['PEG'] @entity4202 :: ('D012965', 'Chemical') :: ['NaCl']
@entity970 :: (CHEBI:46793,Chemical) :: ['PEG']
