The @entity43 arise in the @entity2670 ( @entity2670 ) which is the mesothelial covering of the ovary. Studies of @entity1 @entity6694 have been hampered by the small amounts and limited lifespan of this epithelium in culture. @entity2670 cells expressing SV40 large T antigen (Tag) or the HPV genes E6 and E7 have increased growth potentials but lack some of the normal characteristics of @entity2670 . In this study, we used conditional SV40 Tag expression to produce @entity2670 cells with increased proliferative potentials but relatively normal phenotypes. Primary @entity2670 cultures from three @entity1 , one of whom had a @entity5345 mutation, were infected with a temperature-sensitive Tag construct (tsTag), and from these, 28 monoclonal and four polyclonal lines were isolated. The effects of temperature changes were examined in two monoclonal and two polyclonal lines. At the permissive temperature (34 degrees C), these cell lines underwent 52-71 @entity14485 ( @entity14485 ) compared to 15-20 @entity14485 for normal @entity2670 . Nuclear SV40-Tag and @entity876 expression, demonstrated by immunofluorescence, showed that tsTag was uniformly present and biologically active in all lines. At 34 degrees C, culture morphologies ranged from epithelial to mesenchymal. The mean percentage of cells expressing the epithelial differentiation marker, keratin. varied between lines from 20 to 97%. Collagen type III, a mesenchymal marker expressed by @entity2670 in response to explantation into culture, was present in 24-43% of cells. At 39 degrees C, tsTag was inactivated by 2 d while nuclear @entity876 staining diminished to control levels over 2 wk. Over 3 d. the cells assumed more epithelial morphologies, keratin expression reached 85-100% in all lines and collagen expression increased significantly in two lines. The cultures with the @entity5345 mutation expressed the most keratin and the least collage n III at both temperatures. As indicated by beta-galactosidase staining at pH 6.0, changes leading to senescence were initiated at 39 degrees C by 6 h and were present in all cells after 24 h. However, the cells underwent 1-3 @entity14485 over up to 1 wk before growth arrest and widespread cell death, thus providing an experimental system where large numbers of @entity2670 cells with different genetic backgrounds and growth potentials can be studied without the concurrent influence of Tag.

Differentiation and growth potential of XXXX ovarian surface epithelial cells expressing temperature-sensitive SV40 T antigen.

@entity43 :: ('MESH:D010051', 'Disease') :: ['epithelial ovarian carcinomas'] @entity1 :: ('9606', 'Species') :: ['women', 'human'] @entity5345 :: ('672', 'Gene') :: ['BRCA1'] @entity6694 :: ('MESH:D015518', 'Disease') :: ['USE'] @entity14485 :: ('MESH:D005671', 'Disease') :: ['population doublings', 'PD'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity2670 :: ('MESH:D010049', 'Disease') :: ['OSE', 'ovarian surface epithelium']
@entity1 :: (9606,Species) :: ['human']
