UNASSIGNED: Streptococcus is one of the main pathogens that cause @entity291 @entity1271 . They includes into @entity5967 , @entity22193 , and @entity8211 . The GapC protein is a virulence factor that is expressed on the surface of Streptococcus species. GapC is highly antigenic and immunization with GapC confers cross-protection against all three species. Our previous data showed that @entity3534 1-150 of GapC (GapC1-150) of @entity22193 conferred similar immunoprotection compared to full-length GapC. Thus, the present study aimed to construct a recombinant @entity526 XL1-Blue strain that displayed GapC1-150 on its surface, and to investigate the immunogenicity of the surface-localized GapC1-150. To do so, the ompA gene of the @entity526 XL1-Blue strain was replaced with the lpp'-ompA-gapC11-150 or lpp'-ompA genes by    Red recombination, the former of which fused GapC1-150 to an Lpp lipoprotein signal peptide and @entity3534 1-159 of OmpA; the recombinant strains were named XL1-Blue/LOG76 and XL1-Blue/LO11, respectively. GapC1-150 was confirmed to localize to the surface of the XL1-Blue/LOG76 strain by an indirect enzyme-linked immunosorbent assay (ELISA), a fluorescence-activated cell sorter analysis, and laser-scanning confocal microscopy. Then, ICR @entity19 were immunized intramuscularly with the XL1-Blue/LOG76 or XL1-Blue/LO11 strains, or recombinant GapC1-150. The sera of the immunized @entity19 were collected and the anti-GapC1-150 antibody levels were detected by ELISA. Lymphocytes secreting @entity58 and interferon-y were detected by an enzyme-linked ImmunoSpot assay, as was the level of @entity636 level in the supernatant of cultured splenic lymphocytes. The @entity19 immunized with the XL1-Blue/LOG76 strain or GapC1-150 exhibited better cellular and humoral immunity. Lastly, the immunized @entity19 were challenged with @entity8211 , @entity22193 , and @entity5967 strains, and @entity19 that were immunized with the XL1-Blue/LOG76 strain were better protected than those that were immunized with the XL1-Blue/LO11 strain. These results indicate that it is feasible to display GapC1-150 on the @entity526 surface as a vaccine against Streptococcus species.

Immunogenicity of XXXX 1-150 of Streptococcus GapC displayed on the surface of @entity526 .

@entity3534 :: ('MESH:D000596', 'Chemical') :: ['amino acids'] @entity1271 :: ('MESH:D008413', 'Disease') :: ['mastitis'] @entity5967 :: ('1311', 'Species') :: ['S.agalactiae', 'S. agalactiae'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity58 :: ('16189(Tax:10090)', 'Gene') :: ['interleukin (IL)-4'] @entity8211 :: ('1349', 'Species') :: ['S. uberis', 'S.uberis'] @entity636 :: ('16171(Tax:10090)', 'Gene') :: ['IL-17A'] @entity291 :: ('9913', 'Species') :: ['bovine'] @entity22193 :: ('1334', 'Species') :: ['S.dysgalactiae', 'S. dysgalactiae'] @entity526 :: ('562', 'Species') :: ['Escherichia coli', 'E. coli']
@entity3534 :: (MESH:D000596,Chemical) :: ['amino acids']
