Enterohemorrhagic @entity526 strains cause each year thousands of illnesses, which are sometimes accompanied by the @entity1381 , like in the 2011 outbreak in Germany. For preservation thermal pasteurization is commonly used, which can cause unwanted quality changes. To prevent this high pressure treatment is a potential alternative. Within this study, the 2011 outbreak strain O104:H4, an O157:H7 and a non-pathogenic strain (DSM1116) were tested. The cells were treated in buffer (pH 7 and pH 5) and @entity10786 juice (pH 5.1) in a pressure temperature range of 0.1-500  MPa and 20-70    C. Flow cytometry was used to investigate the pressure impact on cell structures of the strain DSM1116. Both pathogenic strains had a much higher resistance in buffer and @entity10786 juice than the non-pathogenic surrogate. Further, strains cultivated and treated at a lower pH-value showed higher pressure stability, presumably due to variations in the membrane composition. This was confirmed for the strain DSM1116 by flow cytometry. Cells cultivated and treated at pH 5 had a stronger ability to retain their membrane potential but showed higher rates of membrane permeabilization at pressures <200  MPa compared to cells cultivated and treated at pH 7. These cells had the lowest membrane permeabilization rate at around 125  MPa, possibly denoting that variations in the @entity844 composition and membrane fluidity contribute to this stabilization phenomenon.

Comparative study on the high pressure inactivation behavior of the Shiga toxin-producing XXXX O104:H4 and O157:H7 outbreak strains and a non-pathogenic surrogate.

@entity844 :: ('CHEBI:35366', 'Chemical') :: ['fatty acid'] @entity10786 :: ('4039', 'Species') :: ['carrot'] @entity526 :: ('562', 'Species') :: ['Escherichia coli'] @entity1381 :: ('MESH:D006463', 'Disease') :: ['hemolytic uremic syndrome']
@entity526 :: (562,Species) :: ['Escherichia coli']
