@entity1 with the B-cell @entity5734 ( @entity5734 ) exhibit a skewed T-cell repertoire with oligoclonal expression or absence of many members of the T-cell receptor (TCR) BV gene families. To evaluate whether interferon-alpha ( @entity3027 ) therapy would not only restore normal hematopoiesis, but also the abnormal T-cell repertoire, we studied T lymphocytes from a cohort of @entity5734 @entity1 treated by @entity3027 in the past, at initiation, and at several intervals up to 6 years of @entity3027 treatment. The junctional regions from 22 TCRBV gene families were analyzed after polymerase chain reaction amplification of cDNA (RT-PCR) using family specific primers. In all seven @entity1 improvement of the skewed T-cell repertoire was not seen until 2 years of treatment. It consisted of disappearance of oligoclonal subpopulations and (polyclonal) reappearance of absent TCRBV gene families. The RT-PCR results were correlated with the TCRBV protein expression using TCRBV-specific monoclonal antibodies. T lymphocytes from four @entity1 with active @entity5734 contained large expansions of particular TCRBV-expressing cells (up to 25% of the CD3+ cells; 600 to 700/microL whole blood), which decreased during @entity3027 therapy in both @entity1 tested. Finally, restoration of the TCR repertoire matched normalization of the functional immune repertoire as measured by proliferative, helper, and cytotoxic T-lymphocyte precursor frequencies against major histocompatibility complex-unrelated individuals. In conclusion, oligoclonal bands of TCRBV gene families found by RT-PCR correspond with a dramatic increase in circulating T lymphocytes expressing the same TCRBV family. Moreover, @entity3027 can restore the skewed T-cell repertoire and suppress persistent T-cell clones upon treatment of the accompanying @entity5 .

Correction of abnormal T-cell receptor repertoire during interferon-alpha therapy in @entity1 with XXXX .

@entity1 :: ('9606', 'Species') :: ['Patients', 'patients'] @entity5734 :: ('MESH:D007943', 'Disease') :: ['malignancy hairy cell leukemia', 'HCL'] @entity5 :: ('MESH:D009369', 'Disease') :: ['malignancy'] @entity3027 :: ('3439', 'Gene') :: ['IFN-alpha']
@entity5734 :: (MESH:D007943,Disease) :: ['hairy cell leukemia']
