The @entity661 ( @entity661 ) produces all of the plant's aerial organs. The @entity661 is established either during embryogenesis or experimentally in in  vitro tissue culture. Although several factors including the Class I KNOTTED1-LIKE HOMEOBOX (KNOXI) proteins, auxin, and cytokinin are known to play essential roles in @entity661 development, the underlying mechanisms of @entity661 formation and maintenance are still largely not understood. Herein we demonstrate that OsARID3, a member of the @entity579 ( @entity579 ) AT-rich Interaction Domain (ARID) family, is required for @entity661 development. Disruption of OsARID3 leads to a defective @entity661 , early seedling lethality, and impaired capacity of in  vitro shoot regeneration. We show that the expression levels of several KNOXI genes and the biosynthetic genes for auxin and cytokinin are significantly altered in the Osarid3 mutant calli. Moreover, we determine that auxin concentrations are increased, whereas cytokinin levels are decreased, in Osarid3 calli. Furthermore, chromatin immunoprecipitation results demonstrate that OsARID3 binds directly to the KNOXI gene OSH71, the auxin biosynthetic genes OsYUC1 and OsYUC6, and the cytokinin biosynthetic genes OsIPT2 and OsIPT7. We also show through electrophoretic mobility shift assays that OsARID3 specifically binds to the AT-rich DNA sequences of the identified target genes. We conclude that OsARID3 is an AT-rich specific DNA-binding protein and that it plays a major role in @entity661 development in @entity579 .

OsARID3, an AT-rich Interaction Domain-containing protein, is required for shoot meristem development in XXXX .

@entity579 :: ('4530', 'Species') :: ['rice', 'Oryza sativa'] @entity661 :: ('MESH:D054549', 'Disease') :: ['SAM', 'shoot apical meristem']
@entity579 :: (4530,Species) :: ['rice']
