1. Dentate gyrus granule cells acutely dissociated from hippocampal slices obtained from chronic @entity1315 ( @entity1315 ) @entity1 displayed a high-voltage activated ( @entity3829 ) @entity141 conductance with a pronounced @entity141 -dependent inactivation. 2. Inactivation time constants and peak HVA @entity141 current (ICa) amplitudes did not differ between perforated patch and whole-cell recordings without added exogenous @entity141 buffers, indicating that the @entity141 -dependent characteristics of ICa inactivation were well preserved in whole-cell recordings. 3. Inactivation time constants correlated with whole-cell ICa, and were increased when @entity141 was replaced with @entity8594 in the external solution or 5 mM @entity18650 was added to the pipette solution. 4. In recordings without added exogenous @entity141 buffers, the time course of ICa inactivation was comparable between @entity1 @entity1315 and kindled @entity35 granule cells. Conversely, the time course of ICa in @entity1 @entity1315 granule cells loaded with 5 mM intracellular BAPTA resembled that observed in buffer-free recordings from control @entity35 neurones. 5. The loss of a putative intraneuronal @entity141 buffer, the @entity141 -binding protein calbindin (CB), from @entity1 granule cells during @entity1315 may result in the pronounced @entity141 -dependent ICa inactivation. This process could serve a neuroprotective role by significantly decreasing @entity141 entry during prolonged trains of action potentials known to occur during @entity410 .

Calcium-dependent inactivation of high-threshold calcium currents in XXXX dentate gyrus granule cells.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity410 :: ('MESH:D012640', 'Disease') :: ['seizures'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity8594 :: ('MESH:C080430', 'Chemical') :: ['Ba2+'] @entity1315 :: ('MESH:D004833', 'Disease') :: ['TLE', 'temporal lobe epilepsy'] @entity3829 :: ('CHEBI:545959', 'Chemical') :: ['HVA'] @entity18650 :: ('MESH:C025603', 'Chemical') :: ['BAPTA'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+']
@entity1 :: (9606,Species) :: ['human']
