The aim of the present study was to investigate the morphological characteristics and pluripotent differentiation potential of @entity1 bone marrow mesenchymal stem cells (hBMMSCs) in  vitro and in  vivo. Bone marrow cells were isolated from a rib fragment of an adult surgical @entity1 , hBMMSCs were isolated based on plastic adherence and expanded ex  vivo and phenotyping was performed. Pluripotent differentiation assays for adipogenesis, myogenesis and osteogenesis were conducted. Hematopoietic reconstruction of sublethally irradiated @entity19 was performed by infusion of hBMMSCs. The gene expression profiles of early and late hBMMSCs were examined. The rate of @entity2279  -positive cells was 31.1% in passage  (P)4 hBMMSCs and 18.6% in P10 hBMMSCs. CD105 and @entity4725 were expressed in 99 and 95% of P25 hBMMSCs, respectively. Lipid droplets appeared at day  18 post induction. For osteogenesis, palpable masses were grossly observed from day  35 post inoculation of hBMMSCs. Hematoxylin and eosin staining further revealed chondrocytes and bone tissues. For myogenesis, at day six post subcutaneous inoculation, hBMMSCs differentiated into myocytes and were positive for myoglobin and @entity9171 . In irradiated @entity19 reconstituted by hBMMSCs, the white blood cell count briefly decreased following irradiation; however, it gradually recovered. In the irradiated @entity19 reconstituted with hBMMSCs, @entity8236  - and @entity22416  -positive cells were detected 72  h post induction. Gene microarray analysis of P7 and P57 hBMMSCs demonstrated that 20  genes were upregulated >2  fold and 40  genes were downregulated >2  fold in P57 hBMMSCs. In conclusion, the isolated HBMMSCs possessed pluripotent differentiation potential and it was feasible and safe to use hBMMSCs within 30  passages.

Isolation and characterization of ex  vivo expanded mesenchymal stem cells obtained from a surgical XXXX .

@entity1 :: ('9606', 'Species') :: ['human', 'patient'] @entity8236 :: ('19264(Tax:10090)', 'Gene') :: ['CD45'] @entity9171 :: ('4654', 'Gene') :: ['MyoD1'] @entity19 :: ('10090', 'Species') :: ['nude mice'] @entity2279 :: ('5175', 'Gene') :: ['CD31'] @entity4725 :: ('7412', 'Gene') :: ['CD106'] @entity22416 :: ('12490(Tax:10090)', 'Gene') :: ['CD34']
@entity1 :: (9606,Species) :: ['patient']
