The @entity1 mineralocorticoid receptor (MR) is a member of the @entity134 -thyroid hormone receptor superfamily, which includes receptors for @entity1968 , @entity560 , and other @entity230 , such as the glucocorticoids (which bind the glucocorticoid receptor, GR). MR and GR, the corticosteroid receptors, share significant homology and are activated by @entity134 binding, resulting in a conformational change, nuclear translocation, and DNA binding. Despite these similarities with GR, the MR remains less well characterized. However, protein components known to be present in the unliganded GR are also likely to be components of the heteromeric MR complex. In the current study, we investigated whether or not @entity2285 , @entity4041 , and the immunophilin @entity23201 are present in the nonsteroid-bound MR complex, because these proteins are known to be present in the unliganded GR complex. The unliganded MR complex was assembled in vitro using reticulocyte lysate and in vivo using the baculovirus overexpression system and Spodoptera frugiperda (Sf9) cells. Western blot analysis revealed the presence of @entity2285 , @entity4041 , and @entity23201 in the unliganded complexes, but @entity4041 and @entity23201 were not detected following exposure to @entity1369 . Electrophoretic mobility shift analysis demonstrated that DNA binding of MR occurred only after treatment with @entity1369 . These studies indicate that proteins associated with the unliganded GR are also present in the unliganded MR complex, and that @entity4041 and @entity23201 dissociate prior to DNA binding in a manner similar to that described for GR. Finally, the stoichiometric analysis of the proteins present within the heteromeric MR complex suggests a divergence between this receptor and the GR.

The unliganded mineralocorticoid receptor is associated with heat shock proteins 70 and 90 and the immunophilin XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity4041 :: ('3320', 'Gene') :: ['hsp90'] @entity1369 :: ('MESH:D000450', 'Chemical') :: ['aldosterone'] @entity2285 :: ('3308', 'Gene') :: ['hsp70'] @entity134 :: ('CHEBI:35341', 'Chemical') :: ['steroid'] @entity23201 :: ('2288', 'Gene') :: ['FKBP-52'] @entity230 :: ('MESH:D013256', 'Chemical') :: ['steroids'] @entity1968 :: ('MESH:D014212', 'Chemical') :: ['retinoic acid'] @entity560 :: ('MESH:D014807', 'Chemical') :: ['vitamin D']
@entity23201 :: (2288,Gene) :: ['FKBP-52']
