1. @entity1 @entity5843 was prepared from a fraction obtained during the large-scale separation of normal @entity1 plasma proteins for clinical use. 2. Sedimentation-equilibrium measurements indicated a molecular weight of 725000. A value of 18.1S was obtained for s(0) (20,w). 3. The dissociation that occurs in the pH range 4.5-2.5 and in the region of neutrality in @entity1133 -containing solutions is consistent with a dimeric structure of the molecule. 4. The effects of the @entity1432 reagents @entity6514 , @entity8488 and @entity898 were investigated over a range of experimental conditions. Distinct components having sedimentation coefficients of 15, 12 and 8.5S were identified. 5. Conditions were found under which limited reduction with @entity1432 liberated a subunit with a molecular weight approximately one-quarter of that of the intact molecule. This subunit retains the serological specificity of the whole molecule.

Thio reduction of XXXX 2 -macroglobulin. The subunit structure.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity5843 :: ('2', 'Gene') :: ['alpha(2)-macroglobulin'] @entity1133 :: ('MESH:D014508', 'Chemical') :: ['urea'] @entity8488 :: ('MESH:D003543', 'Chemical') :: ['mercaptoethylamine'] @entity898 :: ('MESH:D000111', 'Chemical') :: ['N-acetylcysteine'] @entity1432 :: ('CHEBI:29256', 'Chemical') :: ['thiol'] @entity6514 :: ('MESH:D008623', 'Chemical') :: ['mercaptoethanol']
@entity1 :: (9606,Species) :: ['human']
