Vasoactive intestinal peptide ( @entity49240 ) is a prolactin (PRL)-releasing factor whose activity in avian species is believed to be mediated by a specific @entity49240 receptor ( @entity49240 -R). Circulating PRL levels are closely related to hypothalamic @entity49240 immunoreactivity, hypothalamic @entity49240 mRNA content, and hypophysial-portal blood @entity49240 concentrations in @entity233 . In the present study, a @entity233 @entity49240 -R (tVIP-R) cDNA was cloned and its mRNA abundance was quantified in various tissues during different reproductive stages. The 2347-bp tVIP-R cDNA encoded a 457 @entity535 protein, with a predicted Mr of 52 kDa. The full-length cDNA shares approximately 55% similarity with the @entity1 @entity49240 receptor-1. Northern blot analysis revealed that a major 2.7-kb transcript was expressed in laying hen pituitaries. Furthermore, two minor tVIP-R transcripts of 3.7 and 3.4 kb were observed. Semiquantitative reverse transcription polymerase chain reaction (RT-PCR) was performed using RNA from various @entity233 brain and peripheral tissues throughout the reproductive cycle. The steady-state levels of pituitary tVIP-R mRNA changed during the reproductive cycle, whereas mRNA expression in other tissues was not affected. The steady-state levels of tVIP-R mRNA were only affected in the pituitary, whereas mRNA expression in any of the other tissues examined following the immunization of @entity233 against @entity49240 were not affected.

Molecular cloning and expression analysis of the XXXX vasoactive intestinal peptide receptor.

@entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity49240 :: ('100303697(Tax:9103)', 'Gene') :: ['VIP'] @entity1 :: ('9606', 'Species') :: ['mammalian'] @entity233 :: ('9103', 'Species') :: ['turkey', 'turkeys']
@entity233 :: (9103,Species) :: ['turkey']
