PURPOSE: @entity19 lacking the Arf @entity5 -suppressor gene develop @entity2194 reminiscent of persistent hyperplastic primary vitreous (PHPV). The current work explores mechanisms by which Arf promotes eye development, and its absence causes a @entity22933 . METHODS: Chimeric @entity19 were made by fusing wild-type and Arf(-/-) morulae. In these experiments, wild-type cells are identified by transgenic expression of GFP from a constitutive promoter. PCR-based genotyping and quantitative analyses after immunofluorescence staining of tissue and cultured cells documented the relative contribution of wild-type and Arf(-/-) cells to different tissues in the eye and different types of cells in the vitreous. RESULTS: The contributions of the Arf(-/-) lineage to the tail DNA, @entity267 , retina, and retina pigment epithelium (RPE) correlated with each other in wild-type<-->Arf(-/-) chimeric @entity19 . Newborn chimeras had primary @entity1373 , evident as a retrolental mass. The mass was usually present when the proportion of Arf(-/-) cells was relatively high and absent when the Arf(-/-) proportion was low. The @entity5232 - and @entity37751 -expressing cells within the mass arose predominantly from the Arf(-/-) population. Ectopic Arf expression induced smooth muscle proteins in cultured pericyte-like cells, and Arf and @entity37751 expression overlapped in hyaloid vessels. CONCLUSIONS: In the @entity19 model, @entity393 in only a subset of cells causes a @entity22933 . The data indicate that both cell autonomous and non-cell autonomous effects of Arf may contribute to its role in vitreous development.

Persistent hyperplastic primary vitreous due to somatic mosaic deletion of the arf XXXX suppressor.

@entity2194 :: ('MESH:D005128', 'Disease') :: ['eye disease'] @entity22933 :: ('MESH:D054514', 'Disease') :: ['PHPV-like disease'] @entity267 :: ('MESH:D065306', 'Disease') :: ['cornea'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'Mice'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity1373 :: ('MESH:D006965', 'Disease') :: ['vitreous hyperplasia'] @entity5232 :: ('18596(Tax:10090)', 'Gene') :: ['Pdgfrbeta'] @entity393 :: ('MESH:D034381', 'Disease') :: ['loss of Arf'] @entity37751 :: ('20589(Tax:10090)', 'Gene') :: ['Sma']
@entity5 :: (MESH:D009369,Disease) :: ['tumor']
