BACKGROUND: Due to the importance of @entity1003 and @entity1423 in brain- and @entity65 , snake venom proteins that interfere in these processes have received significant attention in recent years considering their potential to be used as models for new drugs. OBJECTIVES: This study aimed at the evaluation of the in  vivo thrombolytic activity of Batroxase, a P-I metalloprotease from Bothrops atrox venom. METHODS: In  vivo thrombolytic activity of Batroxase was tested on a model of @entity49 in @entity35 , with @entity6323 , and vessel wall injury with @entity30059 at 10% for 5  min. After formation of the @entity715 , increasing amounts of Batroxase were administered intravenously. The prescription medication Alteplase (tissue-type plasminogen activator) was used as positive control for thrombolytic activity, while saline was used as negative control. Bleeding time was assessed with a tail bleeding assay. RESULTS: Batroxase presented thrombolytic activity in  vivo in a concentration-dependent manner, with 12  mg/kg of the metalloprotease causing a @entity715 reduction of 80%, a thrombolytic activity very similar to the one observed for the positive control Alteplase (85%). The tail bleeding time was not altered by the administration of Batroxase, while it increased 3.5 times with Alteplase. Batroxase presented fibrinolytic and fibrinogenolytic activities in  vitro, which were inhibited by @entity14445 . CONCLUSION: Batroxase presents thrombolytic activity in  vivo, thus demonstrating a possible therapeutic potential. The inactivation of the metalloprotease by @entity14445 may reduce its activity, but also its potential side effects, as seen for bleeding time.

Evaluation of the in  vivo thrombolytic activity of a metalloprotease from Bothrops atrox venom using a model of XXXX .

@entity6323 :: ('MESH:C563013', 'Disease') :: ['partial stenosis of the inferior vena cava'] @entity1423 :: ('MESH:D001791', 'Disease') :: ['platelet aggregation'] @entity30059 :: ('MESH:C024555', 'Chemical') :: ['ferric chloride'] @entity14445 :: ('24153(Tax:10116)', 'Gene') :: ['alpha 2-macroglobulin'] @entity49 :: ('MESH:D019851', 'Disease') :: ['venous thrombosis'] @entity1003 :: ('MESH:D001778', 'Disease') :: ['blood coagulation'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity715 :: ('MESH:D013927', 'Disease') :: ['thrombus'] @entity65 :: ('MESH:D002318', 'Disease') :: ['cardiovascular diseases']
@entity49 :: (MESH:D019851,Disease) :: ['venous thrombosis']
