BACKGROUND: @entity12325 ( @entity12325 ) enzymes have been shown to play important roles in chemical @entity105 . Reports have indicated that polymorphism in @entity4187 , @entity4914 , @entity5944 , @entity5321 , and @entity5320 are associated with an altered risk of developing @entity5 of the lung, bladder, liver, and colon. @entity5321 plays an important role in @entity167 metabolism and participates in the metabolic activation of various carcinogens, such as @entity1232 . Recently, we found repeated sequences that consist of four alleles (A1, A2, A3, and A4) and six genotypes (A1/A2, A2/A2, A2/A3, A2/A4, A3/A4 and A4/A4) of the @entity5321 gene. In the present study, we investigated whether these polymorphisms are associated with @entity2 and @entity418 . METHODS: DNA was isolated from blood samples of 192 healthy control subjects, 85 @entity1 with @entity2 , and 82 @entity1 with @entity418 in Japanese males. DNA samples were amplified by polymerase chain reaction in the 5'-flanking region of the @entity5321 gene and in exon 12 in the @entity6921 gene. The tandem repeat polymorphisms were examined using DNA fragment analysis. Missense mutations in exon 12 of the @entity6921 gene were examined using fluorescence-based single strand conformational change polymorphism analysis. Written informed consent was obtained from all the subjects. RESULTS: In the @entity5321 gene 5'-flanking region polymorphism, @entity1 with @entity418 showed significantly higher frequency of the A4/A4 genotype compared with the control subjects (p = 0.02), but no difference was found in @entity1 with @entity2 . The frequencies of the mutant @entity6921 *2 allele were significantly higher in @entity1 with @entity418 (27.7%) than in healthy control subjects (7.3%; p< 0.0001; habitual @entity167 drinkers). CONCLUSIONS: The distribution of genotypes and allele frequencies of the tandem repeats of the 5'-flanking region of the @entity5321 gene was significantly different in @entity1 with @entity418 .

Tandem repeat polymorphism of the @entity5321 gene: an association study with XXXX and @entity2 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity12325 :: ('4051', 'Gene') :: ['CYP', 'Cytochrome P-450'] @entity5944 :: ('1548', 'Gene') :: ['CYP2A6'] @entity4187 :: ('1543', 'Gene') :: ['CYP1A1'] @entity5320 :: ('1565', 'Gene') :: ['CYP2D6'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancers'] @entity167 :: ('CHEBI:16236', 'Chemical') :: ['alcohol'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung cancer'] @entity418 :: ('MESH:D004938', 'Disease') :: ['esophageal cancer'] @entity1232 :: ('MESH:D004128', 'Chemical') :: ['N-nitrosodimethylamine'] @entity105 :: ('MESH:D063646', 'Disease') :: ['carcinogenesis'] @entity4914 :: ('1544', 'Gene') :: ['CYP1A2'] @entity6921 :: ('217', 'Gene') :: ['ALDH2'] @entity5321 :: ('1571', 'Gene') :: ['CYP2E1']
@entity418 :: (MESH:D004938,Disease) :: ['esophageal cancer']
