OBJECTIVE: Several fastidious bacteria have been associated with @entity6981 ( @entity6981 ), but their role in lactobacilli recolonization failure is unknown. We studied the effect of 7 @entity6981 -associated bacterial species and 2 @entity10265 species on vaginal colonization with @entity10265 CTV-05 (LACTIN-V). METHODS: Twenty-four @entity1 with @entity6981 were given a 5-day course of @entity868 vaginal gel and then randomized 3:1 to receive either LACTIN-V or placebo applied vaginally once daily for 5 initial consecutive days, followed by a weekly application over 2 weeks. Vaginal swabs for @entity10265 CTV-05 culture and 9 bacterium-specific 16S rRNA gene quantitative polymerase chain reaction assays were analyzed on several study visits for the 18 @entity1 receiving LACTIN-V. RESULTS: Vaginal colonization with CTV-05 was achieved in 61% of the @entity1 receiving LACTIN-V at either day 10 or day 28 visit and 44% at day 28. @entity1 not colonized with CTV-05 had generally higher median concentrations of @entity6981 -associated bacteria compared to those who colonized. Between enrollment and day 28, the median concentration of @entity8439 minimally reduced from 10 to 10 16S rRNA gene copies per swab in @entity1 who colonized with CTV-05 but increased from 10 to 10 in those who failed to colonize (P = 0.19). Similarly, the median concentration of Atopobium spp. reduced from 10 16S rRNA gene copies per swab to below limit of detection in @entity1 who colonized with CTV-05, but increased from 10 to 10 in those who failed to colonize (P = 0.04). The presence of endogenous @entity10265 at enrollment was found to be significantly associated with a reduced odds of colonization with CTV-05 on day 28 (P = 0.003), and vaginal intercourse during the study significantly impaired successful CTV-05 colonization (P = 0.018). CONCLUSION: Vaginal concentration of certain @entity6981 -associated bacteria, vaginal intercourse during treatment, and the presence of endogenous @entity10265 at enrollment predict colonization with probiotic lactobacilli.

Effects of XXXX and sexual intercourse on vaginal colonization with the probiotic @entity10265 CTV-05.

@entity1 :: ('9606', 'Species') :: ['participants', 'women', 'Participants'] @entity868 :: ('MESH:D008795', 'Chemical') :: ['metronidazole'] @entity8439 :: ('2702', 'Species') :: ['Gardnerella vaginalis'] @entity6981 :: ('MESH:D016585', 'Disease') :: ['BV', 'bacterial vaginosis'] @entity10265 :: ('47770', 'Species') :: ['Lactobacillus crispatus', 'Lactobacillus', 'L. crispatus']
@entity6981 :: (MESH:D016585,Disease) :: ['bacterial vaginosis-associated bacteria']
