One important aspect of oxidant injury is the enhancement of neutrophil-endothelial adhesion by oxidants such as @entity273 . Recent studies suggest that @entity842 (NO) can limit oxidant-mediated tissue injury, since inhibitors of endogenous NO synthesis often promote neutrophil-endothelial adhesion. However, less is known about the direct role of exogenous NO in modulating proadhesive effects of oxidants. The objective of this study was to examine how an NO donor modifies @entity273 -mediated adhesion of neutrophils to cultured endothelial cells. @entity1 umbilical vein endothelial cell monolayers were exposed for 30 min to 0-0.1 mM @entity273 with or without the NO donor @entity55202 ( @entity17265 ; 0-0.5 mM), and the adhesion of 51Cr-labeled polymorphonuclear neutrophils (PMNs) was measured in a static adhesion assay. PMN adherence was not altered by either @entity2625 (up to 0.1 mM) or @entity17265 (up to 0.5 mM) alone but was significantly increased by over 300% by coadministration of both 0.1 mM @entity2625 and 0.5 mM @entity17265 . This increase in adhesion with these two agents was correlated with an increase in the presentation of surface @entity8729 but not @entity2071 . Both PMN adhesion and @entity8729 presentation were blocked by 0.1 mM @entity5532 (an @entity68 chelator) and 1 mM @entity1853 (an oxyradical scavenger). @entity6849 , a platelet-activating factor-receptor antagonist (10 microM), also prevented PMN adhesion but not @entity8729 expression. An antibody directed against either @entity8729 or @entity2071 also blocked adhesion. These data indicate that NO may actually exacerbate rather than protect against the inflammatory effects of @entity2625 in some models of @entity32 through the synthesis of platelet-activating factor and the mobilization of @entity8729 .

Exogenous NO enhances XXXX -mediated neutrophil adherence to cultured endothelial cells.

@entity1 :: ('9606', 'Species') :: ['Human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity6849 :: ('MESH:C052518', 'Chemical') :: ['WEB-2086'] @entity17265 :: ('CHEBI:65308', 'Chemical') :: ['SNO'] @entity5532 :: ('MESH:D003676', 'Chemical') :: ['desferrioxamine'] @entity2625 :: ('CHEBI:44785', 'Chemical') :: ['peroxide'] @entity55202 :: ('MESH:C091861', 'Chemical') :: ['spermine-NONOate'] @entity1853 :: ('MESH:D008715', 'Chemical') :: ['methionine'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['hydrogen peroxide'] @entity8729 :: ('6403', 'Gene') :: ['P-selectin'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity2071 :: ('3383', 'Gene') :: ['intercellular adhesion molecule-1'] @entity68 :: ('MESH:D007501', 'Chemical') :: ['iron']
@entity273 :: (MESH:D006861,Chemical) :: ['hydrogen peroxide']
