The gene expression and protein distribution of matrix metalloproteinase (MMP) -2, -9, membrane type-1 MMP ( @entity5781 ), as well as of @entity51948 were analyzed during @entity19 molar development. Immunohistochemical data demonstrated that all the MMPs investigated were expressed in the dental epithelium and mesenchyme. In contrast, gene and protein expression analysis for TIMPs showed that they were differentially expressed. @entity5779 was expressed in the dental epithelium and mesenchyme between @entity9073 and E16 and was transiently up-regulated at @entity9072 , the cap stage. @entity5779 expression was also detected in differentiating odontoblasts. @entity5783 RNA transcripts were found in the peridental and dental mesenchyme, odontoblasts, and ameloblasts. Protein analysis revealed high expression on the lingual side of the dental epithelium and underlying mesenchyme together with transient expression in the enamel knot at @entity9072 and expression in the gingival tissue and enamel matrix postnatally. @entity11289 RNA transcripts were found in discrete regions of the dental epithelium, including at high levels in the cervical loop at E16. Expression was also detected in preodontoblasts at E16 and transiently during ameloblast differentiation. Analysis of the protein distribution revealed a lower level of @entity11289 on the lingual side of the dental epithelium at @entity9072 . @entity5781 was expressed in the dental mesenchyme between @entity9073 and E16, at relatively high levels in the cervical loop at @entity9072 , and in the odontoblasts and ameloblasts. The distinct temporospatial distribution patterns of the TIMPs suggest that these inhibitors play several intrinsic roles during tooth development.

Temporospatial gene expression and protein localization of matrix metalloproteinases and their inhibitors during XXXX molar tooth development.

@entity51948 :: ('21857;21858;21859', 'Gene') :: ['TIMP-1, -2, and -3'] @entity5783 :: ('21858(Tax:10090)', 'Gene') :: ['TIMP-2'] @entity5781 :: ('17387(Tax:10090)', 'Gene') :: ['MT1-MMP'] @entity9072 :: ('114729(Tax:10090)', 'Gene') :: ['E14'] @entity11289 :: ('21859(Tax:10090)', 'Gene') :: ['TIMP-3'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity9073 :: ('114727(Tax:10090)', 'Gene') :: ['E13'] @entity5779 :: ('21857(Tax:10090)', 'Gene') :: ['TIMP-1']
@entity19 :: (10090,Species) :: ['mouse']
