Oocytes from @entity1238 microinjected with RNA isolated from @entity380 adult worms translated antigens recognized by sera from infected @entity35 , @entity1 , and from immunized @entity95 . The pattern of immunoprecipitated proteins analysed by @entity214 - @entity2975 gel electrophoresis was species specific in rats. Serum from infected Fischer rats recognized antigens of 20, 27 and several bands in the 50-60 kDa range whereas serum from infected Brown @entity35 also immunoprecipitated major bands at 29, 43 and 100 kDa. @entity1 infection sera gave a very variable pattern of immunoprecipitation not apparently dependent on the @entity1 ' age. At least 20 different antigenic species could be identified ranging from 14 to 150 kDa. Some S. mansoni antigenic proteins could be isolated from the membrane fraction of the oocytes whereas notably the 29 kDa band was present mainly in the soluble fraction. N-Glycosylation of S. mansoni antigens occurred as evidenced by the effects of @entity2425 treatment and concanavalin A binding. A multiple series of bands between 50 and 60 kDa, present in the membrane fraction, were glycosylated and secreted from the oocytes. Monoclonal antibodies to larval stage surface antigens failed to immunoprecipitate oocyte translation products, but sera absorbed with live schistosomula identified at least three putative surface antigens of 100, 43 and 29 kDa. However, the 29 kDa molecule was neither synthesized into membranes, nor secreted from oocytes.

Translation of XXXX mansoni antigens in @entity1238 oocytes microinjected with mRNA from adult worms.

@entity1 :: ('9606', 'Species') :: ['humans', 'patients', 'Human'] @entity1238 :: ('8355', 'Species') :: ['Xenopus laevis'] @entity2425 :: ('MESH:D014415', 'Chemical') :: ['tunicamycin'] @entity95 :: ('9986', 'Species') :: ['rabbits'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity380 :: ('6183', 'Species') :: ['Schistosoma mansoni'] @entity35 :: ('10116', 'Species') :: ['Norway rats', 'rats'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS']
@entity380 :: (6183,Species) :: ['Schistosoma']
