Vascular smooth muscle cells (VSMC) from spontaneously @entity101 @entity35 (SHR) proliferate faster than those from @entity35 -Kyoto @entity35 (WKY). Therefore regulation of cell cycle progression was examined in VSMC from both strains. Analysis of G1 progression was performed in VSMC synchronized by serum starvation. Double staining for @entity2667 and @entity1603 revealed that G1 progression was faster in SHR as compared with WKY. Indeed, 59+/-6% of VSMC from SHR but only 14+/-10% of those from WKY had left G1 phase after 24 hours of mitogenic stimulation. Moreover, 15+/-2% of SHR cells had already completed the cycle at this time point. Western blot analysis demonstrated that the level of cyclin D, @entity12002 , and cyclin A was higher in SHR cells progressing through G1 phase, whereas expression of @entity23305 as well as the cyclin-dependent kinase inhibitors @entity7504 and @entity5447 were similar in the two groups. Consistent with a higher level of cyclins, the activity of @entity23305 was more pronounced in SHR cells. Analysis of G2 progression was performed in VSMC synchronized by treatment with @entity7091 and revealed an additional difference in cell cycle regulation between SHR and WKY. Indeed, the level of cell division cycle kinase 2 was higher in cells from SHR, whereas that of its catalytic partner cyclin B was similar. Consistent with this pattern of expression, the activity of cell division cycle kinase 2 was more pronounced in VSMC from SHR as compared with WKY. Thus, these data demonstrate that the different proliferation of VSMC from SHR and WKY is related to a different progression in G1 phase as the result of the expression of cyclin D, cyclin A, and @entity12002 as well as a different progression in G2 phase caused by expression of cell division cycle kinase 2.

Different cell cycle regulation of vascular smooth muscle in XXXX .

@entity1603 :: ('MESH:D001973', 'Chemical') :: ['bromodeoxyuridine'] @entity5447 :: ('83571(Tax:10116)', 'Gene') :: ['p27'] @entity7504 :: ('24525(Tax:10116)', 'Gene') :: ['p21'] @entity101 :: ('MESH:D006973', 'Disease') :: ['hypertensive'] @entity2667 :: ('MESH:D011419', 'Chemical') :: ['propidium iodide'] @entity7091 :: ('MESH:D016590', 'Chemical') :: ['aphidicolin'] @entity12002 :: ('25729(Tax:10116)', 'Gene') :: ['cyclin E'] @entity35 :: ('10116', 'Species') :: ['Wistar', 'rats'] @entity23305 :: ('362817(Tax:10116)', 'Gene') :: ['cyclin-dependent kinase 2']
@entity101 :: (MESH:D006973,Disease) :: ['genetic hypertension']
