UNASSIGNED: @entity1 and porcine @entity3993 is caused by the larval stage of the flatworm @entity8785 (Cestoda). The protein extracts of @entity8785 cysts are complex mixtures including cyst's and host proteins. Little is known about the influence of using different detergents in the efficiency of solubilization-extraction of these proteins, including relevant antigens. Here, we describe the use of CHAPS, ASB-14 and @entity6000 , alone or in combination in the extraction buffers, as a strategy to notably increase the recovery of proteins that are usually left aside in insoluble fractions of cysts. Using buffer with CHAPS alone, 315 protein spots were detected through 2D-PAGE. A total of 255 and 258 spots were detected using buffers with @entity6000 or ASB-14, respectively. More protein spots were detected when detergents were combined, i.e., 2% CHAPS, 1% @entity6000 and 1% @entity70005 allowed detection of up to 368 spots. Our results indicated that insoluble fractions of @entity8785 cysts were rich in antigens, including several glycoproteins that were sensitive to metaperiodate treatment. Host proteins, a common component in protein extracts of cysts, were present in larger amounts in soluble than insoluble fractions of cysts proteins. Finally, antigens present in the insoluble fraction were more appropriate as a source of antigens for diagnostic procedures.

Differential antigenic protein recovery from XXXX cyst tissues using several detergents.

@entity1 :: ('9606', 'Species') :: ['Human'] @entity3993 :: ('MESH:D003551', 'Disease') :: ['cysticercosis'] @entity70005 :: ('MESH:C506587', 'Chemical') :: ['ASB-14'] @entity6000 :: ('MESH:D017830', 'Chemical') :: ['Triton X-100'] @entity8785 :: ('6204', 'Species') :: ['Taenia solium', 'T. solium']
@entity8785 :: (6204,Species) :: ['Taenia solium']
