PURPOSE: To introduce a practical method of subretinal injection in @entity19 and evaluate injection-induced @entity1713 ( @entity1713 ) and damage using a dynamic imaging system, electrophysiology, and histology. METHODS: After full dilation of a 2-month-old C57BL/6J @entity19 pupil, the @entity267 near the limbus was punctured with a 30   -gague disposable beveled needle. A 33   -gauge blunt needle was inserted through the corneal perforation into the anterior chamber, avoiding the lens before going deeper into the vitreous cavity, and penetrating the inner retina to reach the subretinal space. The @entity19 were divided into four groups: in group 1, about 80-100% of the retina was filled with subretinally injected solution; in group 2, approximately 50-70% of the retina was filled with injected solution; in group 3, the procedures were stopped before solution injection; and non-injected eyes were used as the negative control in group 4. An optical coherence tomography (OCT) imaging system was used to monitor @entity5995 reattachment during the first three days following the injections. Histological and functional changes were examined by light microscopy and electroretinography (ERG) at five weeks post-injection. RESULTS: After a short-term training, a 70% success rate with 50% or more coverage (i.e., retinal @entity4530 occupied 50% or more retinal area and filled with the injected solution) with minimal injection-related damages can be achieved. Bleb formation was associated with @entity1713 ( @entity1713 ) between the neuroretina and the retinal pigment epithelium (RPE) layer. Partial @entity1713 could be observed at post-injection day 1, and by day 2 most of the retina had reattached. At 5 weeks post-injection, compared to uninjected control group 4, the b-wave amplitudes of ERG decreased 22% in group 1, 16% in group 2, and 7% in group 3; the b-wave amplitudes were statistically different between the uninjected group and the groups with either 50-70% or 80-100% coverage. The subretinal injection-induced @entity1713 reattached and became stable at five weeks post-injection, although some photoreceptor damage could still be observed in and around the injection sites, especially in 80-100% coverage group. CONCLUSIONS: Trans-corneal subretinal injection is effective and practical, although subretinal injection-related damages can cause some morphological and functional loss.

Trans-Corneal Subretinal Injection in XXXX and Its Effect on the Function and Morphology of the Retina.

@entity267 :: ('MESH:D065306', 'Disease') :: ['cornea'] @entity4530 :: ('MESH:D001768', 'Disease') :: ['blebs'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity1713 :: ('MESH:D012163', 'Disease') :: ['RD', 'retinal detachment'] @entity5995 :: ('MESH:D012172', 'Chemical') :: ['retinal']
@entity19 :: (10090,Species) :: ['Mice']
