Since 1998, @entity14886 (ARV) @entity281 has been detected in broiler and breeding @entity1573 flocks in Tunisia. The genotype of avian reoviruses was established using simple and rapid approaches. Reverse transcription PCR (RT-PCR) on both sigma C (  C) and sigma B (  B)-encoding genes followed by restriction fragment length polymorphism (RFLP) analyses were used to better characterize Tunisian isolated strains. The RT-PCR amplified fragments of 738 and 540 bp for   C- and   B-encoding genes, respectively, of 15 ARV Tunisian strains. DNA fragments amplified from S 1133 vaccine and isolated strains were digested with different restrictions enzymes. RFLP on the   C gene indicated that the field isolates and the S 1133 vaccine strain have identical profiles when separately digested with TaqI, @entity9379 , DdeI, and HincII. Considering the   B gene, RFLP profiles were identical with RsaI, BclI, DpnII, and NciI restriction enzymes for all the strains. However, using MseI and AciI enzymes, it was shown that all tested isolates could be clearly distinguished from the vaccine strain. ARV strains could be classified in groups with strong relatedness. Strain-typing based on cleavage site results are in agreement with ARV clustering based on @entity4334 sequences of both the   C and   B genes. RT-PCR-RFLP provides a simple and a rapid approach for genotyping ARV isolates, especially when a large number of isolates are being studied. Additionally, this approach may also determine whether a new variant strain has been introduced into a flock or if a given virus strain is being spread from one flock to another.

Genotyping and Classification of Tunisian Strains of XXXX using RT-PCR and RFLP Analysis.

@entity9379 :: ('6690', 'Gene') :: ['PstI'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity1573 :: ('9031', 'Species') :: ['chicken'] @entity14886 :: ('38170', 'Species') :: ['avian reovirus'] @entity4334 :: ('D009711', 'Chemical') :: ['nucleotide']
@entity14886 :: (38170,Species) :: ['Avian Reovirus']
