BACKGROUND: The current medical treatment of @entity46 , a common @entity66 , is still associated with a high recurrence rate. To establish an appropriate in-vivo model to evaluate new therapeutic strategies we validated the nude @entity19 model for the intraperitoneal cultivation of @entity1 endometrial tissue. METHODS: @entity1 endometrium of the proliferative phase was implanted into the peritoneal cavity of normal cycling and ovariectomized athymic @entity19 and of cycling non-obese @entity6 (NOD)-severe @entity791 ( @entity791 ) @entity19 . Morphology, proliferation, differentiation, and angiogenesis in the ectopic endometrium at different time points after implantation was investigated. RESULTS: Adhesion of endometrial fragments was observed from day 2 onwards. The lesions persisted for up to 28 days revealing a well preserved glandular morphology. The glandular epithelium maintained cytokeratin expression even after 14 days of culture. With progressing culture, glands exhibited @entity3111 staining in combination with a decrease of surrounding stromal cells. @entity7944 could be demonstrated throughout the investigated period of 28 days, whereas expression of @entity2931 and @entity1420 receptors was maintained only in endometriotic lesions grown in cycling but not in ovariectomized @entity19 . Neoangiogenesis occurred from day 4 onwards, independent from the intraperitoneal localization of the ectopic lesions. CONCLUSIONS: This in-vivo model is a promising tool to test the effect of compounds such as different hormone agonists/antagonists or anti-angiogenic factors to develop new therapeutic concepts in @entity46 .

Peritoneal XXXX : validation of an in-vivo model.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity66 :: ('MESH:D004194', 'Disease') :: ['gynaecological disease'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetic'] @entity3111 :: ('22352(Tax:10090)', 'Gene') :: ['vimentin'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity2931 :: ('CHEBI:50114', 'Chemical') :: ['oestrogen'] @entity1420 :: ('MESH:D011374', 'Chemical') :: ['progesterone'] @entity7944 :: ('MESH:C536309', 'Disease') :: ['Proliferation of glandular epithelium'] @entity46 :: ('MESH:D004715', 'Disease') :: ['endometriosis'] @entity791 :: ('MESH:D000163', 'Disease') :: ['combined immuno-deficiency', 'SCID']
@entity46 :: (MESH:D004715,Disease) :: ['endometriosis']
