Over expression of @entity1262 ( @entity1262 ) in @entity5 cells often results in highly aggressive, multi-drug resistant (MDR) phenotype. Such @entity5 are very difficult to treat with conventional therapy and often lead to failure of the treatment. In this work, we fabricated surface engineered hybrid lipid nanoparticles grafted with novel AL- @entity15773 polymer by mineralization technique. AL- @entity15773 graft polymer was prepared by covalent conjugation of alendronate sodium and hyaluronic acid. Compritol ATO 888 and capmule MCM C8 hybrid lipid mix was employed to prepare irinotecan containing nanostructured lipid carrier (NLC) by using functional excipients with @entity1262 inhibition activity. AL- @entity15773 was successfully grafted over NLC-Ir (uncoated irinotecan loaded NLC) by calcium-assisted mineralization. @entity15773 -NLC-Ir (hyaluronic acid coated irinotecan loaded NLC) particles have a nanoscale size of 386  2.2nm along with a zeta potential value of 19.7  1.2mV. NLC-Ir as well as @entity15773 -NLC-Ir showed a slow and sustained drug release. In vitro cell line studies performed on HT-29 and @entity1 @entity694 cells revealed a reduced IC50 even in MDR cells. Flowcytometry studies demonstrated the capability of the developed nanocarriers to deliver the @entity1262 substrate moieties in MDR @entity5 cells. Furthermore, the targeting potential of @entity15773 -NLC was confirmed by CLSM studies. The cell line studies also revealed that NLC formulation had a potential of inhibiting @entity1262 by affecting ATPase activity and @entity1262 gene expression.

Surface engineered nanostructured lipid carriers for targeting XXXX : Part I. Synthesis, characterization and in vitro investigation.

@entity1 :: ('9606', 'Species') :: ['Colo-320'] @entity1262 :: ('5243', 'Gene') :: ['MDR1', 'P-glycoprotein', 'P-gp'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer', 'tumors'] @entity15773 :: ('MESH:C537629', 'Disease') :: ['HA'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon cancer']
@entity5 :: (MESH:D009369,Disease) :: ['MDR tumor']
