This study aims to detect the mRNA of @entity876 b and   133p53 isoforms in three @entity356 cell lines and tissues of @entity3621 , @entity4726 , @entity356 , or paracancerous area. Nested reverse transcription PCR was used to detect the mRNA of @entity876 b and   133p53 isoforms in tissues of @entity3621 , chronic @entity4726 , @entity356 cell lines (SGC-7901, MKN45, KATO III), @entity356 , and @entity75 . The amplified products were shown by agarose gel electrophoresis. The expression difference among various tissues was analyzed by x(2) tests. The positive rates of    133p53 mRNA were 73.3% (11/15) in @entity356 and 20% (3/15) in paracancerous tissue, whereas the positive rates of p53b mRNA were 20% (3/15) in @entity356 and 66.7% (10/15) in paracancerous tissue. The difference between @entity420 and paracancerous tissues was significant (P<0.05). The positive rates of    133p53 mRNA were 25% (5/20), 50% (15/30), and 75% (15/20), respectively, in @entity3621 , @entity4726 , and @entity356 ; the positive rates of p53b mRNA were 65% (13/20), 33.3% (10/30), and 25% (5/20), respectively, in @entity3621 , @entity4726 , and @entity356 . The difference between @entity420 and @entity3621 samples was significant (P<0.05). Both @entity876 b and    133p53 mRNAs were positive in MKN45; only p53b mRNA was detected in SGC7901; neither @entity876 b nor    133p53 mRNA was detected in KATO III.    133 @entity876 and p53b, which are possible indicators for the diagnosis and biological therapy of @entity356 , were expressed differentially in different gastric tissues.

Expression of XXXX b and   133 @entity876 isoforms in different gastric tissues.

@entity4726 :: ('MESH:D005757', 'Disease') :: ['atrophic gastritis'] @entity75 :: ('MESH:D001927', 'Disease') :: ['paracancerous lesion'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity3621 :: ('MESH:D005756', 'Disease') :: ['superficial gastritis'] @entity420 :: ('MESH:D000230', 'Disease') :: ['adenocarcinoma'] @entity356 :: ('MESH:D013274', 'Disease') :: ['gastric cancer', 'gastric adenocarcinoma', 'gastric carcinoma']
@entity876 :: (7157,Gene) :: ['p53']
