Replication of @entity9925 ) is restricted to a few sites, including the brain and spinal cord. However, this neurotropism is not conserved in cultured cells. Monkey kidney cells become susceptible to @entity281 after cultivation in vitro, and cell lines of monolayer cultures from almost any tissue of primates are susceptible to @entity281 . These observations suggest that cellular changes during cultivation are required for acquisition of susceptibility. The molecular basis for the cellular changes during this process is not known. We investigated the relationship between PV susceptibility and interferon (IFN) response in primary cultured kidney and liver cells derived from @entity19 expressing @entity1 receptor and in several primate cell lines. Both kidneys and liver in vivo showed rapid IFN response within 6 h postinfection. However, monkey and @entity19 kidney cells in culture and primate cell lines, which were susceptible to PV, did not show such rapid response or showed no response at all. On the other hand, primary cultured liver cells, which were partially resistant to @entity281 , showed rapid IFN induction. The loss of IFN inducibility in kidney cells was associated with a decrease in expression of IFN-stimulated genes involved in IFN response. @entity19 kidney cells pretreated with a small dose of IFN, in turn, restored IFN inducibility and resistance to PV. These results strongly suggest that the cells in culture acquire PV susceptibility during the process of cultivation by losing rapid IFN response that has been normally maintained in extraneural tissues in vivo.

Role of the alpha/beta interferon response in the acquisition of susceptibility to XXXX by kidney cells in culture.

@entity19 :: ('10090', 'Species') :: ['Mouse', 'mouse', 'transgenic mice'] @entity281 :: ('MESH:D007239', 'Disease') :: ['PV infection', 'infection'] @entity1 :: ('9606', 'Species') :: ['human PV'] @entity9925 :: ('138950', 'Species') :: ['poliovirus (PV']
@entity9925 :: (138950,Species) :: ['poliovirus']
