AIMS: There is a growing body of evidence that cyclo-oxygenase 2 (COX-2) plays an important role in @entity105 and angiogenesis of @entity1 @entity5 . The present study aims to compare COX-2 expression in @entity1 @entity0 and adjacent non-cancerous tissue (ANCT), and to identify any correlation between COX-2 and @entity7 expression. METHODS: Total cellular RNA was extracted from frozen breast tissue samples according to standard methodology. The mRNA copy numbers for COX-2 and @entity7 189 ( @entity7 -189) were determined 40 infiltrating @entity189 and 40 matched ANCT specimens using quantitative RT-PCR and TaqMan methodology. RESULTS: The COX-2 mRNA copy number per microg of RNA was two-fold higher in ANCT compared with the cancerous tissue (p = 0.01). Median mRNA copy number was 5.44 x 10(6) for ANCT and 2.30 x 10(6) for @entity5 , (ANCT range: 1 x 10(6) to 4.12x 10(7)) ( @entity5 range: 1.29 x 10(5) to 1.07 x 10(7)). There was a significant correlation between COX-2 and @entity7 -189 mRNA copy numbers in the @entity5 specimens (correlation coefficient = 0.5528, p = 0.0076). CONCLUSIONS: COX-2 mRNA is overexpressed in both @entity1 @entity0 and ANCT. We found higher levels in the matched ANCT which suggests that paracrine effects may be important in the role of COX-2 in mammary @entity105 . Furthermore, our results indicate that in @entity1 @entity0 COX-2 overexpression is linked to @entity7 -189 overexpression and therefore @entity5 angiogenesis.

The mRNA expression of cyclo-oxygenase-2 (COX-2) and @entity7 ( @entity7 ) in XXXX @entity0 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancers', 'breast cancer'] @entity7 :: ('7422', 'Gene') :: ['vascular endothelial growth factor', 'VEGF'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer', 'tumours', 'tumour'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinomas'] @entity105 :: ('MESH:D063646', 'Disease') :: ['carcinogenesis']
@entity1 :: (9606,Species) :: ['human']
