In airway epithelial cells, extracellular @entity855 ( @entity855 (o)) stimulates an initial transient increase in intracellular @entity141 concentration that is followed by periodic increases in intracellular @entity141 concentration ( @entity141 oscillations). The characteristics and mechanism of these @entity855 -induced @entity141 responses were studied in primary cultures of @entity95 tracheal cells with digital video fluorescence microscopy and the @entity141 -indicator dye @entity593 . The continual presence of @entity855 (o) at concentrations of 0.1-100 microM stimulated @entity141 oscillations that persisted for 20 min. The frequency of the @entity141 oscillations was found to be dependent on both @entity855 (o) concentration and intrinsic sensitivity of each cell to @entity855 (o). Cells exhibited similar @entity141 oscillations to extracellular @entity856 ( @entity856 (o)), but the oscillations typically occurred at lower @entity856 (o) concentrations. The @entity855 -induced @entity141 oscillations were abolished by the phospholipase C inhibitor @entity4385 and by the endoplasmic reticulum @entity141 -pump inhibitor @entity2426 but were maintained in @entity141 -free medium. These results are consistent with the hypothesis that in airway epithelial cells @entity855 (o) and @entity856 (o) act via P2U purinoceptors to stimulate @entity141 oscillations by the continuous production of @entity671 and the oscillatory release of @entity141 from internal stores. @entity855 -induced @entity141 oscillations of adjacent individual cells occurred independently of each other. By contrast, a mechanically induced intercellular @entity141 wave propagated through a field of @entity141 -oscillating cells. Thus @entity141 oscillations and propagating @entity141 waves are two fundamental modes of @entity141 signaling that exist and operate simultaneously in airway epithelial cells.

Intracellular calcium oscillations induced by XXXX in airway epithelial cells.

@entity671 :: ('MESH:D015544', 'Chemical') :: ['inositol 1,4,5-trisphosphate'] @entity2426 :: ('MESH:D019284', 'Chemical') :: ['thapsigargin'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity856 :: ('MESH:D014544', 'Chemical') :: ['UTP'] @entity593 :: ('MESH:D016257', 'Chemical') :: ['fura 2'] @entity4385 :: ('MESH:C060229', 'Chemical') :: ['U-73122'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity855 :: (MESH:D000255,Chemical) :: ['ATP']
