Five main type H1 histones have been described in @entity1 ( @entity26644 -H1.5) in addition to the testis specific type @entity27539 and the replacement subtype H1 degrees, which is found mainly in highly differentiated cells. We have isolated this whole complement of H1 genes and have studied the expression of the seven @entity1 H1 subtype genes in several cell lines. The RNAase protection assay was used to discriminate between the very similar transcripts derived from the seven H1 subtype genes. With the exception of H1.2 and H1.4, we found substantial differences between the H1 mRNA levels in the different cell lines tested. No @entity26644 mRNA was detected in most of the cell lines and just a low level of @entity26644 mRNA was found in @entity1 testis. In contrast to the differential patterns of the other subtypes, H1.2 and H1.4 were in all cells expressed at a high level, indicating a basal function compared with the other H1 histones. Because differences in the timing of H1 protein subtype synthesis have been reported, we have analyzed the kinetics of accumulation of H1 subtypes in synchronized HeLa cells and observed that all H1 subtypes examined (H1 degrees, H1.2-H1.5) were expressed in a replication-dependent manner. The analysis showed a differential rise of mRNA levels during S-phase, from four-fold (H1 degrees) to 15-fold (H1.5). Our results may point at a specific function of each subtype and suggest that expression of the H1 histone subtype genes depends on common S-phase-depent factors as well as on individual regulatory systems. Thus, the data presented here provide a basis for further analysis of the regulation and function of the complex H1 gene and protein family.

Varied expression patterns of XXXX H1 histone genes in different cell lines.

@entity1 :: ('9606', 'Species') :: ['human', 'man'] @entity27539 :: ('3010', 'Gene') :: ['H1t'] @entity26644 :: ('3024', 'Gene') :: ['H1.1']
@entity1 :: (9606,Species) :: ['human']
