The @entity470 ( @entity470 ) plays an important role in the development and @entity263 and its overexpression is associated with decreased survival. With progression, @entity263 cells switch from @entity4179 ( @entity4179 ) to @entity310 ( @entity7790 ) synthesis, which contributes to autocrine growth and unrestrained proliferation. To define the molecular mechanisms involved in the regulation of @entity470 expression by @entity4179 and @entity7790 we studied three @entity1 @entity263 cell lines, @entity342 -responsive (LNCaP) and -unresponsive (DU145 and @entity5049 ). Here we show that @entity7790 stabilized @entity470 mRNA two- to threefold in all three cell lines, whilst @entity4179 stabilized @entity470 mRNA approximately twofold in LNCaP and DU145 cells, but not in @entity5049 cells. Both ligands increased @entity470 transcription in LNCaP and DU145 cells, with less effect in @entity5049 cells. In all three cell lines @entity4179 reduced total @entity470 protein levels more than @entity7790 , but this was associated with a greater increase in de novo protein synthesis with @entity4179 compared to @entity7790 . Only @entity4179 , however, shortened @entity470 protein stability (half-life decreased from 5 h to 120 min), resulting in rapid disappearance of newly synthesized @entity470 protein. Both ligands increased total LNCaP and DU145 cell numbers. These studies demonstrate that the @entity4179 - and @entity7790 -induced upregulation of @entity470 mRNA and protein in @entity1 @entity263 cell lines is complex and occurs at multiple, transcriptional and post-transcriptional levels. Taken together, these data provide novel insight into the molecular mechanisms by which @entity7790 would preferentially maintain an autocrine loop in @entity1 @entity263 cells. Furthermore, this work suggests that in @entity1 @entity263 cells ligand-specific differential intracellular trafficking of the @entity470 plays a major role in regulating its expression.

Complex post-transcriptional regulation of @entity4179 -receptor expression by @entity4179 and @entity7790 in XXXX @entity263 cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity263 :: ('MESH:D011471', 'Disease') :: ['progression of prostate cancer', 'prostate cancer'] @entity470 :: ('1956', 'Gene') :: ['EGFR', 'epidermal growth factor receptor'] @entity310 :: ('7124', 'Gene') :: ['transforming growth factor alpha'] @entity7790 :: ('7039', 'Gene') :: ['TGF-alpha'] @entity4179 :: ('1950', 'Gene') :: ['epidermal growth factor', 'EGF'] @entity342 :: ('CHEBI:50113', 'Chemical') :: ['androgen'] @entity5049 :: ('57332', 'Gene') :: ['PC3']
@entity1 :: (9606,Species) :: ['human']
