UNASSIGNED: The present study was aimed at discovering DNA copy number alterations (CNAs) involved in the @entity105 and at understanding their clinicopathological significances in the Korean population. DNA copy numbers were analyzed using Agilent 244K or 400K array comparative genomic hybridization (aCGH) in fresh-frozen @entity5 and matched normal tissues from 40 @entity356 @entity1 . Some of the detected CNA regions were validated using multiplex ligation-dependent probe amplification (MLPA) in six of the 40 @entity1 and customized Agilent 60K aCGH in an independent set of 48 @entity356 . The mRNA levels of genes at common CNA regions were analyzed using quantitative real-time PCR. Copy number gains were more common than losses across the entire genome in @entity5 tissues compared to matched normal tissues. The mean number of alterations per case was 64 for gains and 40 for losses, and the median aberration length was 44016 bp for gains and 4732 bp for losses. Copy number gains were frequently detected at 7p22.1 (20%), 8q24.21 (27%-30%), 8q24.3 (22%-48%), 13q34 (20%-31%), and 20q11-q13 (25%-30%), and losses at 3p14.2 (43%), 4q35.2 (27%), 6q26 (23%), and 17p13.3 (20%-23%). CNAs at 7p22.1, 13q34, and 17p13.3 have not been reported in other populations. Most of the copy number losses were associated with down-regulation of mRNA levels, but the correlation between copy number gains and mRNA expression levels varied in a gene-dependent manner. In addition, copy number gains tended to occur more commonly in @entity5 than in @entity5 . In conclusion, the present study suggests that copy number gains at 8q24 and 20q11-q13 and losses at 3p14.2 may be common events in @entity356 but CNAs at 7p22.1, 13q34, and 17p13.3 may be Korean-specific.

Copy Number Gains at 8q24 and 20q11-q13 in XXXX Are More Common in Intestinal-Type than Diffuse-Type.

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'intestinal-type cancers', 'diffuse-type cancers'] @entity1 :: ('9606', 'Species') :: ['patients'] @entity105 :: ('MESH:D063646', 'Disease') :: ['carcinogenesis of stomach'] @entity356 :: ('MESH:D013274', 'Disease') :: ['gastric cancer', 'gastric cancers']
@entity356 :: (MESH:D013274,Disease) :: ['Gastric Cancer']
