PURPOSE: @entity2184 continues to present problems when attempting to predict disease progression. This study attempts to identify markers indicative of the biological characteristics of cells isolated from samples of @entity2184 , including adhesion ( @entity2071 ), immune reactivity (MHC Class I and II), cell cycle control ( @entity1722 , @entity517 ) and apoptosis control ( @entity588 , @entity876 ) using dual parameter (DNA/MoAb) flow cytometry. METHODS: Sixty-three fresh tissue samples from @entity320 were taken at enucleation. Samples were assayed for DNA content and cell cycle, the above antibodies together with positive (PHM-5) and negative (2 degrees FITC Ab) controls. The clinical parameters sex, age, @entity5 location, cell type, @entity5 volume and presence of @entity3 were compared with the results and analysed with the non-parametric Mann-Whitney U-t-test. RESULTS: @entity2071 expression proved to be the most clinically relevant, being present on a higher proportion of cells in @entity5 > 2000 mm3 (median 38, n = 19) compared with the smaller @entity5 < 2000 mm3 (median 17, n = 26) (p = 0.0015). @entity3 was present in 11 @entity1 and did not correlate with any of the surface markers. @entity517 , @entity1722 and MHC Class II expression were associated with cell type, all showing greater expression in spindle cell @entity5 than mixed/epithelial types. CONCLUSION: These results show flow cytometry as a quick, easy method to provide a 'phenotypic profile' for these @entity5 , and identifies cell cycle control and adhesion molecule expression as important areas for further investigation. @entity1722 and @entity588 positivity was typically seen on over 60% cells in each sample, indicating two potential targets for therapeutic intervention.

The correlation between cell surface markers and clinical features in XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity588 :: ('596', 'Gene') :: ['bcl-2'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumours', 'tumour'] @entity517 :: ('4609', 'Gene') :: ['c-myc', 'C-myc'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity2184 :: ('MESH:C536494', 'Disease') :: ['Uveal melanoma', 'uveal melanoma'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastases', 'Metastatic disease'] @entity2071 :: ('3383', 'Gene') :: ['ICAM-1'] @entity320 :: ('MESH:D008545', 'Disease') :: ['choroidal melanomas'] @entity1722 :: ('2064', 'Gene') :: ['c-erbB-2']
@entity320 :: (MESH:D008545,Disease) :: ['choroidal malignant melanomas']
