CONTEXT: In cases of damaged corneal endothelium cells (CECs) of the eye, transplantation of cultured vascular endothelial cells (VECs) may be a viable method to restore transparency. AIMS: To evaluate the viability of replacing damaged primate CECs with cultured allogeneic VECs. SUBJECTS AND METHODS: @entity2608 VECs (RMVECs) were cultured and proliferating cells were labeled with @entity1603 ( @entity1603 ) in vitro. RMs of the experimental group (n = 6) underwent manual Descemettt membrane stripping with transplantation of RMVECs labeled with @entity1603 ; those in the control group received manual Descemetnt membrane stripping without transplantation. Postoperative evaluations included the transparency and appearance of the corneal graft; distribution and ultrastructural changes of RMVECs on the inner surface of the @entity267 using scanning and transmission electron microscopy, and immunohistological identification of @entity1603 . RESULTS: At 90 days postsurgery, the corneal grafts of the monkeys in the experimental group retained better transparency than those of the controls, without @entity7499 or @entity2372 . A layer of cells with positive @entity1603 staining was found on the posterior surface of the treated corneas in the experimental group, while there was no VEC structure in corneal grafts from the monkeys of the control group. CONCLUSIONS: RMVECs can grow on the posterior surface of the @entity267 without Descemet's membrane. Cultured and transplanted RMVECs appeared similar in ultrastructure. VECs can provide a barrier to maintain corneal dehydration and transparency to some extent.

Transplantation of cultured @entity2608 vascular endothelial cells to allogeneic XXXX with stripping of Descemet's membrane.

@entity1603 :: ('MESH:D001973', 'Chemical') :: ['BrdU', 'bromodeoxyuridine'] @entity267 :: ('MESH:D065306', 'Disease') :: ['cornea'] @entity7499 :: ('MESH:D016510', 'Disease') :: ['corneal neovascularization'] @entity2372 :: ('MESH:C562399', 'Disease') :: ['bullous keratopathy'] @entity2608 :: ('9544', 'Species') :: ['Rhesus monkey']
@entity267 :: (MESH:D065306,Disease) :: ['cornea concomitant']
