UNASSIGNED: Recent advances demonstrate a critical yet poorly understood role for the pancreatic stellate cell (PSC) in the pathogenesis of @entity1689 ( @entity1689 ) and @entity788 ( @entity788 ). Progress in this area has been hampered by the availability, fidelity, and/or reliability of in vitro models of PSCs. We examined whether outgrowth cultures from @entity1 surgical specimens exhibited reproducible phenotypic and functional characteristics of PSCs. PSCs were cultured from surgical specimens of healthy pancreas, @entity1689 and @entity788 . Growth dynamics, phenotypic characteristics, soluble mediator secretion profiles and co-culture with @entity788 cells both in vitro and in vivo were assessed. Forty-seven primary cultures were established from 52 attempts, demonstrating universal a-smooth muscle actin and glial fibrillary acidic protein but negligible epithelial surface antigen expression. Modification of culture conditions consistently led to cytoplasmic lipid accumulation, suggesting induction of a quiescent phenotype. Secretion of growth factors, chemokines and cytokines did not significantly differ between donor pathologies, but did evolve over time in culture. Co-culture of PSCs with established @entity788 cell lines resulted in significant changes in levels of multiple secreted mediators. Primary PSCs co-inoculated with @entity788 cells in a xenograft model led to augmented @entity5 growth and @entity3 . Therefore, regardless of donor pathology, outgrowth cultures produce PSCs that demonstrate consistent growth and protein secretion properties. Primary cultures from pancreatic surgical specimens, including @entity5 , may represent a reliable source of @entity1 PSCs.

Primary outgrowth cultures are a reliable source of XXXX pancreatic stellate cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity788 :: ('MESH:D010190', 'Disease') :: ['pancreatic cancer', 'PC'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'malignancies'] @entity1689 :: ('MESH:D050500', 'Disease') :: ['CP', 'chronic pancreatitis'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis']
@entity1 :: (9606,Species) :: ['human']
