BACKGROUND: @entity2708 yield has been badly affected by different insects and weed competition. In Past Application of multiple chemicals is required to manage insects and weed control was achieved by different conventional means, such as hand weeding, crop rotation and polyculture, because no synthetic chemicals were available. The control methods shifted towards high input and target-oriented methods after the discovery of synthetic herbicide in the 1930s. To utilise the transgenic approach, @entity2708 plants expressing the codon-optimised CEMB GTGene were produced in the present study. RESULTS: Local @entity2708 variety CEMB-02 containing Cry1Ac and Cry2A in single cassette was transformed by synthetic codon-optimised @entity4251 synthase gene cloned into pCAMBIA 1301 vector under 35S promoter with Agrobacterium tumifaciens. Putative transgenic plants were screened in MS medium containing 120    mol/L @entity4252 . Integration and expression of the gene were evaluated by PCR from genomic DNA and ELISA from protein. A 1.4-kb PCR product for @entity4252 and 167-bp product for Cry2A were obtained by amplification through gene specific primers. Expression level of @entity4252 and Bt proteins in two transgenic lines were recorded to be 0.362, 0.325    g/g leaf and 0.390, 0.300    g/g leaf respectively. FISH analysis of transgenic lines demonstrates the presence of one and two copy no. of Cp4 EPSPS transgene respectively. Efficacy of the transgene Cp4 EPSPS was further evaluated by @entity4252 spray (41  %) assay at 1900  ml/acre and insect bioassay which shows 100  %mortality of insect feeding on transgenic lines as compared to control. CONCLUSION: The present study shows that the transgenic lines produced in this study were resistant not only to insects but also equally good against 1900  ml/acre field spray concentration of @entity4252 .

Herbicide-resistant XXXX ( @entity2708 ) plants: an alternative way of manual weed removal.

@entity4252 :: ('CHEBI:27744', 'Chemical') :: ['Glyphosate', 'glyphosate'] @entity4251 :: ('CHEBI:57701', 'Chemical') :: ['5-enolpyruvylshikimate-3-phosphate'] @entity2708 :: ('3635', 'Species') :: ['cotton', 'Cotton']
@entity2708 :: (3635,Species) :: ['cotton']
