A sequenced collection of plasmid-borne random fusions of @entity526 DNA to a @entity22291 luxCDABE reporter was used as a starting point to select a set of 689 nonredundant functional gene fusions. This group, called LuxArray 1.0, represented 27% of the predicted transcriptional units in @entity526 . High-density printing of the LuxArray 1.0 reporter strains to membranes on agar plates was used for simultaneous reporter gene assays of gene expression. The cellular response to @entity802 perturbation was analyzed using this format. As expected, fusions to promoters of LexA-controlled SOS-responsive genes dinG, dinB, uvrA, and ydjM were found to be upregulated in the presence of @entity802 . In addition, six fusions to genes not previously known to be induced by @entity802 were also reproducibly upregulated. The responses of two of these, fusions to oraA and yigN, were induced in a LexA-dependent manner by both @entity802 and @entity1138 , identifying these as members of the LexA regulon. The responses of the other four were neither induced by @entity1138 nor dependent on lexA function. Thus, the promoters of ycgH, intG, rihC, and a putative operon consisting of lpxA, lpxB, rnhB, and dnaE were not generally DNA damage responsive and represent a more specific response to @entity802 . These results demonstrate that cellular arrays of reporter gene fusions are an important alternative to DNA arrays for genomewide transcriptional analyses.

LuxArray, a high-density, genomewide transcription analysis of XXXX using bioluminescent reporter strains.

@entity1138 :: ('MESH:D016685', 'Chemical') :: ['mitomycin C'] @entity22291 :: ('29488', 'Species') :: ['Photorhabdus luminescens'] @entity526 :: ('562', 'Species') :: ['Escherichia coli', 'E. coli'] @entity802 :: ('MESH:D009268', 'Chemical') :: ['nalidixic acid']
@entity526 :: (562,Species) :: ['Escherichia coli']
