UNASSIGNED: @entity70198 is a major bioactive constituent isolated from Anoectochilus formosanus and is investigated as an antihyperlipidemic candidate. In this study, a rapid, sensitive, and reliable bioanalytical method was developed for the determination of @entity70198 in @entity35 plasma using hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC- @entity15 / @entity15 ). The plasma sample was pretreated with 1% @entity1543 , followed by protein precipitation with @entity1626 : @entity1042 (70:30). Chromatographic separation was performed on a HILIC @entity3440 column (2.1mm*100mm, 3 m). The mobile phases consisted of 0.1% @entity1543 in distilled water (solvent A) and 0.1% @entity1543 in @entity1626 (solvent B). A gradient program was used at a flow rate of 0.2mL/min. For mass spectrometric detection, the multiple reaction monitoring mode was used; the MRM transitions were m/z 265.2 > m/z 102.9 for @entity70198 and m/z 163.3 > m/z 132.1 for the internal standard (IS) @entity1239 in the positive ionization mode. A calibration curve was constructed in the range of 2-500ng/mL. The intra- and interday precision and accuracy were within 5%. The HILIC- @entity15 / @entity15 method was specific, accurate, and reproducible and was successfully applied in a pharmacokinetic study of @entity70198 in @entity35 .

Development of a hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the determination of @entity70198 , an antihyperlipidemic candidate, in XXXX plasma and its application to pharmacokinetic studies.

@entity1042 :: ('MESH:D000432', 'Chemical') :: ['methanol'] @entity1239 :: ('MESH:D009538', 'Chemical') :: ['nicotine'] @entity1626 :: ('MESH:C032159', 'Chemical') :: ['acetonitrile'] @entity70198 :: ('MESH:C422363', 'Chemical') :: ['kinsenoside', 'Kinsenoside'] @entity3440 :: ('MESH:D012822', 'Chemical') :: ['silica'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity15 :: ('MESH:D009103', 'Disease') :: ['MS'] @entity1543 :: ('MESH:D019342', 'Chemical') :: ['acetic acid']
@entity35 :: (10116,Species) :: ['rat']
