UNASSIGNED: The antitumor activity of carbonic anhydrase (CA) inhibitors is attributed to their ability to induce a state of intracellular acidification. In fact, acidic intracellular pH was demonstrated to upregulate several @entity5 suppressor proteins and increase the activity of many chemotherapies. The present study aimed to investigate the antitumor activity of the CA inhibitor, @entity15438 , in combination with @entity1138 and to study the effect of these drugs on tumoral @entity15049 ( @entity15049 ) as well as @entity5 cell proliferation and apoptosis. @entity5 were induced by subcutaneous inoculation of Ehrlich's @entity189 (EAC) cells in female @entity19 . @entity19 were treated with @entity15438 (3, 10, or 30  mg/kg/day, i.p.) and/or @entity1138 (1  mg/kg, i.p.) weekly for 3  weeks. Treatment with @entity1138 increased @entity15049 level in EAC solid @entity5 in @entity19 . Likewise, treatment with @entity15438 upregulated @entity15049 and @entity899 while downregulated @entity1379 . Both drug therapies increased tumoral @entity7349 , @entity185 , and @entity8383 cleavage in addition to decreasing the proliferative Ki-67-stained nuclear fraction. Indeed, a synergistic effect was detected between @entity1138 and @entity15438 . The current data demonstrated that the antitumor activity of @entity1138 and @entity15438 was, at least in part, mediated through stimulating tumoral expression of @entity15049 and enhancing @entity5 apoptosis.

@entity15438 synergizes the antitumor activity of XXXX against Ehrlich's carcinoma grown in @entity19 : role of @entity15049 .

@entity15438 :: ('MESH:C062765', 'Chemical') :: ['dorzolamide'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'Solid tumors', 'tumors'] @entity19 :: ('10090', 'Species') :: ['mice', 'Mice'] @entity1138 :: ('MESH:D016685', 'Chemical') :: ['mitomycin C'] @entity899 :: ('22060(Tax:10090)', 'Gene') :: ['p53'] @entity189 :: ('MESH:D002277', 'Disease') :: ['ascites carcinoma'] @entity185 :: ('12367(Tax:10090)', 'Gene') :: ['caspase 3'] @entity7349 :: ('12371(Tax:10090)', 'Gene') :: ['caspase 9'] @entity1379 :: ('12043(Tax:10090)', 'Gene') :: ['bcl-2'] @entity8383 :: ('11545(Tax:10090)', 'Gene') :: ['PARP-1'] @entity15049 :: ('56338(Tax:10090)', 'Gene') :: ['thioredoxin-interacting protein', 'TXNIP']
@entity1138 :: (MESH:D016685,Chemical) :: ['mitomycin C']
