We have demonstrated previously that protein kinase Calpha (PKCalpha) plays a key role in regulating phospholipase D (PLD) activation by @entity2036 and the @entity4621 @entity1359 in Madin-Darby @entity235 kidney (MDCK-D1) cells. In the current work, we investigated PLD activation in MDCK-D1 cells triggered by the adrenergic receptor agonist @entity1425 and its mechanism of activation. @entity1425 , acting through the alpha1-adrenergic receptor subtype, promoted transient translocation of PKCalpha and more prolonged translocation of PKCepsilon to the membrane fraction, indicating activation of these two isoforms. In addition, @entity1425 promoted activation of PLD, as shown by a sustained accumulation of @entity33361 . All of these events were blocked by pretreatment of cells with the alpha1-adrenergic antagonist @entity3159 . D609, an inhibitor of @entity2434 hydrolysis, blocked translocation of PKCalpha and PKCepsilon but did not inhibit PLD activation. Unlike results with @entity4515 , or with the P2 purinergic receptor agonist @entity855 , @entity1425 -stimulated PLD activity was not inhibited in MDCK-D1 cells in which PKCalpha expression is attenuated by an antisense cDNA construct or in cells in which PKC activity was inhibited by 1 microM GF 109203X. However, PLD activation by @entity1425 was abolished by concomitant incubation of cells with the @entity460 chelator @entity4527 . These data, together with previous results, are consistent with the hypothesis that in MDCK-D1 cells, @entity1425 acting on alpha1-adrenergic receptors, promotes a rapid increase in cytosolic @entity141 that promotes activation of PLD through an as-yet poorly defined mechanism. The data demonstrate that different types of G protein-linked receptors that activate PLD can mediate this activation in either a PKC activation-dependent or -independent manner within a single cell type.

Stimulation of phospholipase D via alpha1-adrenergic receptors in Madin-Darby XXXX kidney cells is independent of PKCalpha and -epsilon activation.

@entity2036 :: ('MESH:D009711', 'Chemical') :: ['nucleotides'] @entity235 :: ('9615', 'Species') :: ['canine'] @entity1425 :: ('MESH:D004837', 'Chemical') :: ['epinephrine', 'Epinephrine'] @entity4527 :: ('MESH:D004533', 'Chemical') :: ['EGTA'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+'] @entity4515 :: ('CHEBI:60755', 'Chemical') :: ['PMA'] @entity1359 :: ('CHEBI:37537', 'Chemical') :: ['phorbol-12-myristate-13-acetate'] @entity2434 :: ('MESH:D010713', 'Chemical') :: ['phosphatidylcholine'] @entity4621 :: ('CHEBI:37532', 'Chemical') :: ['phorbol ester'] @entity33361 :: ('MESH:C051521', 'Chemical') :: ['phosphatidylethanol'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity3159 :: ('MESH:D011224', 'Chemical') :: ['prazosin'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity235 :: (9615,Species) :: ['canine']
