Regulatory T cells (Tregs) play critical roles in restricting T cell-mediated @entity32 . In the skin, this is dependent on expression of selectin ligands required for leukocyte rolling in dermal microvessels. However, whether there are differences in the molecules used by Tregs and proinflammatory T cells to undergo rolling in the skin remains unclear. In this study, we used spinning disk confocal microscopy in @entity3744 -GFP @entity19 to visualize rolling of endogenous Tregs in dermal postcapillary venules. Tregs underwent consistent but low-frequency rolling interactions under resting and inflamed conditions. At the early stage of the response, Treg adhesion was minimal. However, at the peak of @entity32 , Tregs made up 40% of the adherent @entity1961 (+) T cell population. In a multiple challenge model of @entity56 , rolling of Tregs and conventional @entity1961 (+) T cells was mostly dependent on overlapping contributions of P- and @entity24780 . However, after a second challenge, rolling of Tregs but not conventional @entity1961 (+) T cells became P-selectin independent, and Tregs showed reduced capacity to bind P-selectin. Moreover, inhibition of @entity24780 at this time point resulted in exacerbation of @entity32 . These findings demonstrate that in this multiple challenge model of @entity32 , Treg selectin binding capacity and the molecular basis of Treg rolling can be regulated dynamically.

Regulatory T Cells Dynamically Regulate Selectin Ligand Function during Multiple Challenge Contact XXXX .

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity24780 :: ('20339(Tax:10090)', 'Gene') :: ['E-selectin'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity1961 :: ('12504(Tax:10090)', 'Gene') :: ['CD4'] @entity56 :: ('MESH:D004342', 'Disease') :: ['contact hypersensitivity'] @entity3744 :: ('20371(Tax:10090)', 'Gene') :: ['Foxp3']
@entity56 :: (MESH:D004342,Disease) :: ['Hypersensitivity']
