Overexpression of @entity2247 and enhanced synthesis of @entity1421 ( @entity1421 ) have been implicated in @entity1 endometrial pathologies. To investigate the molecular role of @entity2247 , the Ishikawa @entity1 endometrial epithelial cell line was stably transfected with the pIRES2 vector containing @entity2247 cDNA in either the sense or antisense directions. @entity1421 concentrations were significantly elevated in the cells transfected with the @entity2247 sense compared with wild-type cells or cells transfected with the antisense cDNA (P < 0.01). Elevated @entity1421 synthesis was associated with enhanced expression and signaling of @entity1421 receptors (EP). cDNA array analysis revealed differential expression of @entity10815 between the @entity2247 sense and antisense cells. @entity10815 RNA and protein expression was 6.7- and 2.1-fold lower in the @entity2247 sense compared with @entity2247 antisense cells respectively. @entity10815 is known to cleave plasminogen to the potent antiangiogenic factor @entity26557 . To investigate differential @entity26557 generation, conditioned media from @entity2247 sense, @entity2247 antisense and wild-type cells were incubated with plasminogen and subsequently subjected to Western blot analysis. In comparison to wild-type cells, the cleavage of plasminogen to @entity26557 was abolished when incubated in @entity2247 sense cells conditioned media and elevated when incubated in @entity2247 antisense cells conditioned media. Coincubation of plasminogen with the @entity10815 inhibitor @entity14622 inhibited the cleavage of plasminogen to @entity26557 in the @entity2247 antisense conditioned media. These data demonstrate that @entity2247 exerts a negative feedback on the expression of @entity10815 . This in turn reduces the generation of the antiangiogenic factor @entity26557 , hence promoting a proangiogenic environment.

Cyclooxygenase-2 overexpression inhibits @entity10815 -mediated cleavage of plasminogen to the potent antiangiogenic factor XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity10815 :: ('1509', 'Gene') :: ['cathepsin D', 'Cathepsin D'] @entity14622 :: ('MESH:C031375', 'Chemical') :: ['pepstatin A'] @entity1421 :: ('MESH:D015232', 'Chemical') :: ['PGE2', 'prostaglandin E2'] @entity26557 :: ('MESH:D043168', 'Chemical') :: ['angiostatin'] @entity2247 :: ('4513', 'Gene') :: ['cyclooxygenase (COX)-2', 'COX-2']
@entity26557 :: (MESH:D043168,Chemical) :: ['angiostatin']
