OBJECTIVES: This study aimed to systematically analyze changes in mitochondrial-related protein expression in @entity150 cells and @entity5 -associated fibroblasts and to investigate the characteristics of @entity150 cell energy metabolism. METHODS: In this study, we utilized the following techniques to achieve the objectives: (1) a co-culture system of @entity150 cells and fibroblasts was built using a microfluidic chip as a three-dimensional culture system; (2) the concentration of @entity880 in the medium from the different groups was determined using an automatic micro-plate reader; (3) a qualitative analysis of mitochondria-related protein expression was performed by immunofluorescent staining; and (4) a quantitative analysis of mitochondrial-associated protein expression was conducted via Western blot. SPSS software was utilized to analyze the data. RESULTS: (1) Determination of @entity880 concentration: The @entity880 concentration was determined to be highest in the experimental group, followed by the @entity1 cell control group and then the fibroblast control group. (2) Qualitative results: In the control group, the mitochondrial-related protein fluorescence intensity was higher in the fibroblasts compared with the @entity5 cells, and the fluorescence intensity of the fibroblasts was reduced compared with the experimental group. The mitochondrial-related protein fluorescence intensity of the @entity5 cells was higher in the experimental group compared with the control group, and the opposite results were obtained with the fibroblasts. (3) Quantitative results: The expression of mitochondria-related proteins was higher in fibroblasts compared with @entity5 cells in the control group, and the opposite results were obtained in the experimental group (P<0.05). The expression of mitochondria-related proteins was increased in @entity5 cells in the experimental group compared with the control group; the opposite results were observed for the fibroblasts (P<0.05). CONCLUSIONS: The energy metabolism of @entity150 cells does not parallel the "Warburg effect" because even under sufficient @entity26 conditions, @entity5 cells still undergo glycolysis. @entity150 cells also have an efficient oxidative phosphorylation process wherein @entity5 cells promote glycolysis in adjacent interstitial cells, thereby causing increased formation of nutritional precursors. These high-energy metabolites are transferred to adjacent @entity5 cells in a specified direction and enter the Krebs Cycle. Ultimately, oxidative phosphorylation increases, and sufficient @entity855 is produced.

The study of energy metabolism in XXXX cells in co-culture conditions using a microfluidic chip.

@entity1 :: ('9606', 'Species') :: ['T24'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity150 :: ('MESH:D001749', 'Disease') :: ['bladder tumor', 'Bladder cancer', 'bladder cancer'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactic acid'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity150 :: (MESH:D001749,Disease) :: ['bladder cancer']
