A new aqueous nanoparticle system has been developed using complex coacervation employing the oppositely charged polymers @entity12279 ( @entity8681 ) and dextran sulfate (DS), with @entity17562 as a stabilizing agent. @entity2379 ( @entity11015 ) was loaded into the nanoparticles as a model drug. The nanoparticles contained @entity8681 and DS in the weight ratio of approximately 1:2. They possessed a zeta potential of approximately +30 mV and demonstrated a narrow size distribution in the range 100-600 nm with a polydispersity index of 0.2. Electron microscopy revealed spherical nanocapsules with a smooth surface. Very favorable drug entrapment and recovery efficiencies of up to 85% were routinely observed. Processing parameters, such as the pH of the @entity8681 solutions, ratio of the two polymers, as well as the concentrations of DS and @entity17562 , all played a significant role in controlling particle size. Dissolution studies demonstrated a fast release that is dependent on the model drug solubility. The @entity11015 -loaded nanoparticles displayed no @entity137 in tissue culture in contrast to free drug and were almost as efficacious as free drug in killing @entity5132 . Advantages of this simple technique are (1) ease of manufacturing and mild preparation conditions, (2) employment of completely aqueous processing conditions, (3) use of biocompatible polymers that can be prepared aseptically, (4) ability to control their size, and (5) a high level of drug entrapment.

Formulation and characterization of XXXX - @entity12279 -dextran sulfate nanoparticles.

@entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity2379 :: ('MESH:D000666', 'Chemical') :: ['Amphotericin B'] @entity12279 :: ('MESH:D011094', 'Chemical') :: ['polyethylenimine'] @entity5132 :: ('5476', 'Species') :: ['Candida albicans'] @entity8681 :: ('CHEBI:53231', 'Chemical') :: ['PEI'] @entity17562 :: ('MESH:D019287', 'Chemical') :: ['zinc sulfate'] @entity11015 :: ('CHEBI:40719', 'Chemical') :: ['AmB']
@entity2379 :: (MESH:D000666,Chemical) :: ['amphotericin B']
