The @entity671 receptor ( @entity15304 ) is an intracellular @entity141 channel that plays a role in the regulation of insulin secretion. In @entity35 isolated pancreatic islets the expression of types I, II and III @entity15304 mRNA was identified by reverse transcriptase-polymerase chain reaction and confirmed by cDNA cloning and sequencing. The islet ratios of types I, II and III @entity15304 mRNA to @entity4644 mRNA were 0.08 +/- 0.02, 0.08 +/- 0.03 and 0.25 +/- 0.04 respectively. Types I, II and III @entity15304 mRNA were also expressed in @entity35 (RINm5F) and @entity19 (betaHC9) pancreatic beta-cell lines, and @entity35 cerebellum. Type III @entity15304 mRNA was quantitatively the most abundant form in @entity35 islets and RINm5F cells. In betaHC9 cells, types II and III @entity15304 mRNA were expressed at similar levels, and in much greater abundance than type I mRNA. Type III was the least abundant @entity15304 mRNA in cerebellum. Culture of betaHC9 cells for 5 days at 2.8 and 25 mM @entity413 , or RINm5F cells for 7 days at 5.5 and 20 mM @entity413 , resulted in significantly enhanced expression of type III, but not types I and II, @entity15304 mRNA in the cells at the higher @entity413 concentrations. During short-term (0.5-2 h) incubations, betaHC9 cell type III @entity15304 mRNA levels increased in response to @entity413 in a time- and concentration-dependent manner. @entity4316 inhibited the @entity413 response. @entity6525 also stimulated betaHC9 cell type III @entity15304 mRNA expression in a concentration-dependent manner, whereas @entity897 and @entity10546 were without effect. The different levels of expression of mRNA for three @entity15304 isoforms in islets and @entity9608 cells, and the influence of @entity413 and @entity6525 on the expression of type III mRNA, suggests that nutrient metabolism plays a role in the regulation of this gene and that the function of @entity15304 subtypes may be unique with each playing a distinct role in beta-cell signal transduction and insulin secretion.

Characterization of @entity671 receptor isoform mRNA expression and regulation in XXXX pancreatic islets, RINm5F cells and betaHC9 cells.

@entity671 :: ('MESH:D015544', 'Chemical') :: ['inositol 1,4,5-trisphosphate'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity10546 :: ('MESH:D019325', 'Chemical') :: ['3-O-methylglucose'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity4644 :: ('81822(Tax:10116)', 'Gene') :: ['beta-actin'] @entity9608 :: ('MESH:D007340', 'Disease') :: ['insulinoma'] @entity4316 :: ('MESH:D003609', 'Chemical') :: ['Actinomycin D'] @entity6525 :: ('MESH:C013082', 'Chemical') :: ['alpha-ketoisocaproic acid', 'Alpha-ketoisocaproic acid'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+'] @entity15304 :: ('25262(Tax:10116)', 'Gene') :: ['InsP3R'] @entity897 :: ('MESH:D003847', 'Chemical') :: ['2-deoxyglucose']
@entity35 :: (10116,Species) :: ['rat']
