INTRODUCTION: In this study, we aimed at identifying Candida isolates obtained from blood, urine, tracheal secretion, and nail/ @entity272 from cases attended at the Hospital Universit  rio de Londrina over a 3-year period and at evaluating @entity2380 susceptibilities of the isolates. METHODS: Candida isolates were identified by polymerase chain reaction (PCR) using species-specific forward primers. The in vitro @entity2380 susceptibility test was performed according to EUCAST-AFST reference procedure. RESULTS: Isolates were obtained from urine (53.4%), blood cultures (19.2%), tracheal secretion (17.8%), and nail/ @entity272 (9.6%). When urine samples were considered, prevalence was similar in @entity1 (45.5%) and in @entity1 (54.5%) and was high in the age group >61 years than that in younger ones. For blood samples, prevalence was high in neonates (35%) and advanced ages (22.5%). For nail and skin samples, prevalence was higher in @entity1 (71.4%) than in @entity1 (28.6%). @entity5132 was the most frequently isolated in the hospital, but Candida species other than @entity5132 accounted for 64% of isolates, including predominantly @entity9841 (33.2%) and @entity7409 (19.2%). The trend for non-albicans Candida as the predominant species was noted from all clinical specimens, except from urine samples. All Candida isolates were considered susceptible in vitro to @entity2380 with the exception of isolates belonging to the intrinsically less-susceptible species @entity7408 . CONCLUSIONS: Non-albicans Candida species were more frequently isolated in the hospital. @entity2380 resistance was a rare finding in our study.

Species distribution and in vitro XXXX susceptibility of clinical Candida isolates in a Brazilian tertiary-care hospital over a 3-year period.

@entity1 :: ('9606', 'Species') :: ['women', 'men'] @entity9841 :: ('5482', 'Species') :: ['Candida tropicalis'] @entity2380 :: ('MESH:D015725', 'Chemical') :: ['Fluconazole', 'fluconazole'] @entity7408 :: ('5478', 'Species') :: ['C. glabrata'] @entity7409 :: ('5480', 'Species') :: ['Candida parapsilosis'] @entity5132 :: ('5476', 'Species') :: ['Candida albicans', 'C. albicans'] @entity272 :: ('MESH:D012871', 'Disease') :: ['skin lesions']
@entity2380 :: (MESH:D015725,Chemical) :: ['fluconazole']
