UNASSIGNED: @entity2215 promoter @entity10207 and @entity59802 mutations occur in various @entity5 including @entity150 ( @entity150 ) and may serve as urinary @entity5 markers. However, the mutation association with clinical variables in upper tract @entity189 (UTUCs) is unclear. There is also a lack of sensitive tools to detect the minor mutant @entity2215 promoter in bulk urinary DNA. Here we analyzed 220 UTUC @entity1 [98 with @entity703 ( @entity703 ) and 122 with @entity1698 ( @entity1698 )] and developed a Competitive Allele-Specific TaqMan PCR (castPCR) for urinary assay. We identified @entity10207 or @entity59802 mutations in 42 of 98 (43%) @entity703 and 23 of 122 (19%) @entity1698 @entity5 . Distant metastases were significantly correlated with UTUC @entity1 harboring @entity2215 promoter mutations (P = 0.001). @entity10207 were detected in 6/10 and 9/10 of urine samples from @entity1 with mutation-carrying @entity5 using Sanger sequencing and castPCR, respectively. When urine samples from 70 @entity150 @entity1 were analyzed together, the sensitivity of urinary @entity10207 assay was 89% and 50% for castPCR and Sanger sequencing, respectively (P < 0.001). Collectively, @entity2215 promoter mutations occur in UTUCs with a high frequency in RPCs and predict distant metastasis. castPCR assays of the mutation are a useful tool for urine-based diagnostics of @entity5 .

XXXX promoter mutations are associated with distant metastases in upper tract @entity189 and serve as urinary biomarkers detected by a sensitive castPCR.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity10207 :: ('c|SUB|C|228|T', 'DNAMutation') :: ['C228T'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors', 'urological malignancies', 'malignancies'] @entity59802 :: ('c|SUB|C|250|T', 'DNAMutation') :: ['C250T'] @entity189 :: ('MESH:D002277', 'Disease') :: ['urothelial carcinomas'] @entity150 :: ('MESH:D001749', 'Disease') :: ['BC', 'bladder cancer'] @entity1698 :: ('MESH:D014516', 'Disease') :: ['ureter carcinoma', 'UC'] @entity2215 :: ('7015', 'Gene') :: ['TERT'] @entity703 :: ('MESH:D007680', 'Disease') :: ['renal pelvic carcinoma', 'RPC']
@entity2215 :: (7015,Gene) :: ['TERT']
