BACKGROUND: Our previous study reported that microRNA-26a ( @entity17309 ) inhibited @entity5 progression by inhibiting @entity5 angiogenesis and intratumoral macrophage infiltration in @entity157 ( @entity157 ). The direct roles of @entity17309 on @entity5 cell invasion remain poorly understood. In this study, we aim to explore the mechanism of @entity17309 in modulating epithelial-mesenchymal transition (EMT) in @entity157 . METHODS: In vitro cell morphology and cell migration were compared between the @entity157 cell lines HCCLM3 and HepG2, which were established in the previous study. Overexpression and down-regulation of @entity17309 were induced in these cell lines, and Western blot and immunofluorescence assays were used to detect the expression of EMT markers. Xenograft nude @entity19 models were used to observe @entity5 growth and @entity358 . Immunohistochemical assays were conducted to study the relationships between @entity17309 expression and @entity16719 ( @entity16719 ) and @entity5962 expression in @entity1 @entity157 samples. RESULTS: Down-regulation of @entity17309 in HCCLM3 and HepG2 cells resulted in an EMT-like cell morphology and high motility in vitro and increased in @entity5 growth and @entity358 in vivo. Through down-regulation of @entity16719 expression and up-regulation of @entity5962 expression, @entity17309 inhibited the EMT process in vitro and in vivo. Luciferase reporter assay showed that @entity17309 directly interacted with @entity16719 messenger RNA (mRNA). Furthermore, the expression of @entity17309 was positively correlated with @entity5962 expression and inversely correlated with @entity16719 expression in @entity1 @entity157 tissue. CONCLUSIONS: @entity17309 inhibited the EMT process in @entity157 by down-regulating @entity16719 expression.

MicroRNA-26a suppresses epithelial-mesenchymal transition in @entity1 @entity157 by repressing XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity17309 :: ('407015', 'Gene') :: ['miR-26a'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity16719 :: ('2146', 'Gene') :: ['EZH2', 'enhancer of zeste homolog 2'] @entity5962 :: ('999', 'Gene') :: ['E-cadherin'] @entity358 :: ('MESH:D008171', 'Disease') :: ['pulmonary metastasis'] @entity157 :: ('MESH:D006528', 'Disease') :: ['HCC', 'hepatoma', 'hepatocellular carcinoma']
@entity16719 :: (2146,Gene) :: ['enhancer of zeste homolog 2']
