UNASSIGNED: Class 3 semaphorins were discovered as a family of axon guidance molecules, but are now known to be involved in diverse biologic processes. In this study, we investigated the anti-angiogenic potential of @entity38793 and @entity38791 ( @entity38793 _F) in @entity7492 ( @entity7492 ). @entity7492 is a common @entity5 that involves both vasculogenesis and angiogenesis. Our lab has identified and @entity2695 stem cells (HemSC), @entity413 transporter 1 positive ( @entity1365 (+)) endothelial cells (designated as @entity1365 (sel) cells) based on anti- @entity1365 magnetic beads selection and @entity1365 -negative endothelial cells (named HemEC). We have shown that these types of cells play important roles in hemangiogenesis. We report here that @entity38793 inhibited HemEC migration and proliferation while @entity38791 was able to suppress the migration and proliferation in all three types of cells. Confocal microscopy showed that stress fibers in HemEC were reduced by @entity38793 _F and that stress fibers in HemSC were decreased by @entity38791 , which led to cytoskeletal collapse and loss of cell motility in both cell types. Additionally, @entity38793 _F were able to inhibit @entity7 ( @entity7 )-induced sprouts in all three types of cells. Further, @entity38793 _F reduced the level of p- @entity319 and its downstream p- @entity3963 in HemEC. These results demonstrate that @entity38793 _F inhibit @entity7492 cell proliferation and suppress the angiogenic activities of migration and sprout formation. @entity38793 _F may have therapeutic potential to treat or prevent growth of highly proliferative @entity7492 .

XXXX -derived stem cells and endothelial cells are inhibited by class 3 semaphorins.

@entity2695 :: ('MESH:D006391', 'Disease') :: ['isolated hemangioma'] @entity7492 :: ('MESH:C535860', 'Disease') :: ['IH', 'infantile hemangioma'] @entity38791 :: ('6405', 'Gene') :: ['SEMA3F'] @entity5 :: ('MESH:D009369', 'Disease') :: ['vascular tumor'] @entity7 :: ('7422', 'Gene') :: ['vascular endothelial growth factor', 'VEGF'] @entity3963 :: ('5594', 'Gene') :: ['ERK'] @entity1365 :: ('6513', 'Gene') :: ['GLUT1'] @entity319 :: ('3791', 'Gene') :: ['VEGFR2'] @entity38793 :: ('9723', 'Gene') :: ['SEMA3E'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity7492 :: (MESH:C535860,Disease) :: ['Infantile hemangioma']
