All respiratory long-term facilitation (LTF) is induced by inspiratory-excitatory stimulation, suggesting that LTF needs inspiratory augmentation and is the result of a Hebbian mechanism (coincident pre- and post-synaptic activity strengthens synapses). The present study examined the long-term effects of episodic inspiratory-inhibitory vagus nerve stimulation (VNS) on phrenic nerve activity. We hypothesized that episodic VNS would induce phrenic long-term @entity308 . The results are compared with those obtained following @entity750 receptor antagonism or episodic carotid sinus nerve stimulation (CSNS). Integrated phrenic neurograms were measured before, during and after three episodes of 5 min VNS (50 Hz, 0.1 ms), each separated by a 5 min interval, at a low (approximately 50 microA), medium (approximately 200 microA) or high (approximately 500 microA) stimulus intensity in anaesthetized, vagotomized, neuromuscularly blocked and artificially ventilated @entity35 . Medium- and high-intensity VNS eliminated rhythmic phrenic activity during VNS, while low-intensity VNS only reduced phrenic burst frequency. At 60 min post-VNS, phrenic amplitude was higher than baseline (35 +/- 5% above baseline, mean +/- S.E.M., P < 0.05) in the high-intensity group but not in the low- (-4 +/- 4%) or medium-intensity groups (-10 +/- 15%), or in the high-intensity with @entity3858 group (4 mg kg(-1), i.p.) (-11 +/- 5%). These data, which are inconsistent with our hypothesis, indicate that phrenic-inhibitory VNS induces a @entity750 -dependent phrenic LTF similar to that induced by phrenic-excitatory CSNS (33 +/- 7%) and may require activation of high-threshold afferent fibres. These data also suggest that the synapses on phrenic motoneurons do not use the Hebbian mechanism in this LTF, as these motoneurons were suppressed during VNS.

Episodic phrenic-inhibitory vagus nerve stimulation paradoxically induces phrenic long-term facilitation in XXXX .

@entity35 :: ('10116', 'Species') :: ['rats'] @entity308 :: ('MESH:D003866', 'Disease') :: ['depression'] @entity3858 :: ('MESH:D008784', 'Chemical') :: ['methysergide'] @entity750 :: ('MESH:D012701', 'Chemical') :: ['serotonin']
@entity35 :: (10116,Species) :: ['rats']
