A mild and controlled acute inflammatory reaction in hamster cheek pouch was created without any exogenous, but rather by locally activated and liberated, mediators. A pressure of 60 mm Hg was applied to part of the everted cheek pouch eight times with a 10-min recovery period in between. The microvascular response was followed by intravital microscopy and the permeability changes were monitored with intravital fluoroscopy and intravenous @entity2823 dextran (mol wt 150,000). The number of extravasated polymorphonuclear leukocytes (PMNLs) was calculated by a new, whole tissue, histological technique. In three experimental groups (-)- @entity4183 ( @entity2263 -receptor agonist) 0.05 mg/100 g body wt., @entity4183 0.05 mg/100 g body wt., and @entity3352 2 mg/100 g body wt. was given intravenously before pressure was applied. A fourth group, with no drug given, served as control. There was a rapid increase in the number of @entity2823 dextran leakages and extravasated PMNLs in the control group. @entity3352 almost completely inhibited @entity2823 dextran permeability and extravasation of PMNLs. The @entity2263 agonist markedly diminished the number of @entity2823 dextran leakages for 75 min. The number of extravasated PMNLs was also reduced. Treatment with @entity4183 , which is supposed to have no @entity2263 -receptor effect, gave a slight reduction in number of @entity2823 dextran leakages and almost a complete inhibition of PMNL extravasation. Thus we conclude that @entity3352 is a very potent antiinflammatory agent even in the early phase of @entity32 . @entity4183 diminished the inflammatory response, probably by preventing endothelial gap formation. @entity4183 prevented PMNL extravasation either by interaction with the PMNL itself or with the endothelium.

@entity3352 , XXXX ( @entity2263 agonist), and @entity4183 in acute @entity32 induced by repeated ischemia in hamster cheek pouch.

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity2823 :: ('MESH:D016650', 'Chemical') :: ['FITC'] @entity2263 :: ('10242', 'Gene') :: ['beta 2'] @entity3352 :: ('MESH:D007213', 'Chemical') :: ['indomethacin', 'Indomethacin'] @entity4183 :: ('MESH:D013726', 'Chemical') :: ['terbutaline', '(+)-Terbutaline', '(+)-terbutaline', '(-)-Terbutaline']
@entity4183 :: (MESH:D013726,Chemical) :: ['(-)-terbutaline']
