A new constitutive centromere-specific protein (CENP) has been identified as a result of its recognition as an autoantigen by serum from a @entity1 with @entity19789 . Conventional immunoblotting and two-dimensional double blotting with both this antiserum and a known anti-centromere antiserum showed that this antiserum predominantly recognized a Mr 95,000 protein that is different from all known CENPs. We have named this new protein CENP-G. This protein was detected at the centromeric region throughout the cell cycle. In mitosis, it was restricted to the kinetochore inner plate as shown by immunogold labeling and electron microscopy. The centromeres of some @entity1 chromosomes are known to contain two subfamilies of alpha-satellite DNA. Using immunofluorescence combined with fluorescent in situ hybridization with subfamily-specific DNA probes, we revealed that CENP-G was specifically associated with one of the subfamilies, which we have named @entity6927 , but not the other. The localization and the @entity6927 -specific association suggested that CENP-G may play a role in kinetochore organization and function. Like @entity17353 and C, but unlike @entity17352 , this protein remained with the nuclear matrix after intensive extraction. While @entity17353 is absent from the @entity1 Y chromosome, the existence of CENP-G on the Y chromosome has been proven by immunofluorescence and whole chromosome painting. CENP-G was also detected in @entity435 , Indian muntjac and @entity69782 cells, suggesting that it is conserved in evolution.

CENP-G: a new centromeric protein that is associated with the XXXX satellite DNA subfamily.

@entity1 :: ('9606', 'Species') :: ['human', 'patient'] @entity19789 :: ('MESH:D020252', 'Disease') :: ['gastric antral vascular ectasia disease'] @entity69782 :: ('9886', 'Species') :: ['Chinese muntjac'] @entity17353 :: ('1059', 'Gene') :: ['CENP-B'] @entity17352 :: ('1058', 'Gene') :: ['CENP-A'] @entity435 :: ('CHEBI:42485', 'Chemical') :: ['CHO'] @entity6927 :: ('146', 'Gene') :: ['alpha-1']
@entity6927 :: (146,Gene) :: ['alpha-1']
