OBJECTIVES: We evaluated the effects of irradiation, @entity632 and @entity619 on @entity1 @entity263 cells, investigating whether @entity619 and @entity632 can alter the irradiation cytotoxicity of PC-3 cells. METHODS: We used the @entity1 @entity263 cells, analyzing cell growth with @entity3460 exclusion, indices of the cell cycle with flow cytometry and apoptosis with flow cytometry and analysis of DNA fragmentation on simple agarose gel. RESULTS: @entity632 (100 nM) arrested cell cycle at the G2/M phase, decreased cell growth and produced apoptosis of PC-3 cells in a time-dependent manner. @entity619 (100 nM) increased the distribution of PC-3 cells at G0/G1 phase in the cell cycle, exerting an inhibitory effect on the proliferation of PC-3 cells after 48 and 72 hr, but it did not produce apoptosis. Irradiation (4 Gy) initially arrested cells at the G2/M phase in the cell cycle (24 hr) which was gradually overcome and the PC-3 cells were shifted into G0/G1 phase or apoptosis after 48 and 72 hr. Irradiation decreased the PC-3 cell growth by 40-50% after 48 and 72 hr, respectively. Treatment with @entity632 (100 nM) for 24, 48, and 72 hr after irradiation potentiated irradiation cytotoxicity of PC-3 cells. @entity619 treatment 24 hr before and 24, 48 and 72 hr after irradiation increased the number of surviving PC-3 cells and partially neutralized the irradiation effects on cell cycle. CONCLUSION: @entity632 potentiated while @entity619 partially reversed the irradiation cytotoxicity of PC-3 cells. These data may be of clinical importance for the treatment of hormone @entity263 .

Response of XXXX cells to combination therapy using irradiation with glucocorticoids or @entity632 .

@entity632 :: ('MESH:D004317', 'Chemical') :: ['doxorubicin', 'Doxorubicin'] @entity1 :: ('9606', 'Species') :: ['human'] @entity263 :: ('MESH:D011471', 'Disease') :: ['refractory prostate cancer', 'PC-3 prostate cancer'] @entity619 :: ('MESH:D003907', 'Chemical') :: ['Dexamethasone', 'dexamethasone'] @entity3460 :: ('MESH:D014343', 'Chemical') :: ['trypan blue']
@entity263 :: (MESH:D011471,Disease) :: ['PC-3 prostate cancer']
