UNASSIGNED: Acquired drug resistance is a key factor in the failure of chemotherapy. Due to intratumoral heterogeneity, @entity5 cells depict variations in intracellular drug uptake and efflux at the single cell level, which may not be detectable in bulk assays. In this study we present a droplet microfluidics-based approach to assess the dynamics of drug uptake, efflux and @entity137 in @entity0 cells. An integrated droplet generation and docking microarray was utilized to encapsulate single cells as well as homotypic cell aggregates. Drug-sensitive cells showed greater death in the presence or absence of @entity632 ( @entity3105 ) compared to the drug-resistant cells. We observed heterogeneous @entity3105 uptake in individual drug-sensitive cells while the drug-resistant cells showed uniformly low uptake and retention. @entity3105 -resistant cells were classified into distinct subsets based on their efflux properties. Cells that showed longer retention of extracellular reagents also demonstrated @entity204 . We further observed homotypic fusion of both cell types in droplets, which resulted in increased cell survival in the presence of high doses of @entity3105 . Our results establish the applicability of this microfluidic platform for quantitative drug screening in single cells and multicellular interactions.

Phenotypic drug profiling in droplet microfluidics for better targeting of XXXX .

@entity632 :: ('MESH:D004317', 'Chemical') :: ['Doxorubicin'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity0 :: ('MESH:D001943', 'Disease') :: ['drug-sensitive and drug-resistant breast cancer'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity3105 :: ('D004317', 'Chemical') :: ['Dox'] @entity204 :: ('MESH:D003643', 'Disease') :: ['maximal death']
@entity5 :: (MESH:D009369,Disease) :: ['drug-resistant tumors']
