PURPOSE: Growing evidence suggests that @entity63 may contribute to aging associated @entity1494 and lower urinary tract symptoms. Our goal was to determine the effects of chronic @entity63 on bladder proteomic profiles and characterize downstream signaling pathways. MATERIALS AND METHODS: Bilateral iliac artery @entity731 and chronic @entity1494 were created in male @entity35 . At 8 weeks cystometrograms were obtained. @entity63 and control bladder tissues were then processed for label-free quantitative proteomic analysis. @entity74 ( @entity74 ) and  IPA (Ingenuity   Pathway Analysis) software were used to classify altered proteins in @entity1494 . Western blot was done to confirm differentially expressed proteins. Tissue structure was examined by transmission electron microscopy. RESULTS: @entity63 resulted in detrusor instability and noncompliance. Proteomic analysis revealed a total of 4,277 proteins in @entity63 and 4,602 in control bladder tissues. In @entity63 bladders 359 and 66 proteins were differentially expressed with a greater than twofold and fivefold change, respectively. On @entity74 analysis differentially expressed proteins were associated with molecular signaling mechanisms underlying proteolysis and degenerative processes. Pathway and network analysis of @entity63 tissues suggested that altered proteins are involved in ubiquitination, @entity4027 mediated oxidative stress response, cell death, @entity413 metabolism and cytoskeleton remodeling. Western blot verified changes in 4 representative proteins, including @entity68091 , @entity1589 , @entity1251 and @entity32997 . Altered proteomic profile of the bladder was associated with widespread ultrastructural damage. CONCLUSIONS: Alterations of bladder proteomic profiles in @entity63 may provide new insight into molecular pathways underlying @entity1494 and lower urinary tract symptoms in pelvic @entity731 .

Quantitative Proteomic Analysis of Differentially Expressed Proteins and Downstream Signaling Pathways in Chronic XXXX .

@entity1494 :: ('MESH:D001745', 'Disease') :: ['bladder dysfunction', 'bladder ischemia'] @entity63 :: ('MESH:D007511', 'Disease') :: ['Chronic ischemia', 'Ischemic', 'ischemic', 'ischemia'] @entity68091 :: ('291553(Tax:10116)', 'Gene') :: ['Nedd4l'] @entity32997 :: ('361810(Tax:10116)', 'Gene') :: ['Fkbp5'] @entity4027 :: ('83619(Tax:10116)', 'Gene') :: ['Nrf2'] @entity74 :: ('MESH:D030342', 'Disease') :: ['Gene Ontology', 'GO'] @entity1251 :: ('54232(Tax:10116)', 'Gene') :: ['Ca3'] @entity35 :: ('10116', 'Species') :: ['Sprague Dawley   rats'] @entity731 :: ('MESH:D050197', 'Disease') :: ['atherosclerosis'] @entity1589 :: ('303413(Tax:10116)', 'Gene') :: ['Mpo'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity1494 :: (MESH:D001745,Disease) :: ['Bladder Ischemia']
