OBJECTIVE: To explore the mechanism of Radix Bupleuri (RB) extract in regulating cardiomyocyte apoptosis. Methods The BALB/c @entity19 inoculated with the @entity6475 virus B3m (CVB3m) by peritoneal injection was adopted as @entity2401 model. The experimental @entity19 were divided into the normal control (A), the model (B) and the RB (C) groups. @entity19 in Group C were given 0.2 ml / 10 g of RB extract, 2 mg/ml in concentration by gastrogavage once daily, while equal volume of normal saline was given by gastrogavage to @entity19 in Group A and B. All the @entity19 were sacrificed on the 10th day after modeling. The cardiomyocyte apoptosis was detected by TDT/Dutp nick end labelling (TUNEL). The expression of Fas @entity1379 , and @entity185 protein in myocardium was determined by immunohistochemistry and reverse-transcription polymerse chain reaction (RT-PCR). RESULTS: The apoptotic cell was not found in Group A, but Fas, @entity1379 and @entity185 were expressed in different degree. In Group B, a lot of apoptotic cells could be seen, with expression of Fas, @entity1379 and @entity185 stronger than those in Group A (P < 0.01). Compared with Group B, the apoptotic cardiomyocyte in Group C was significantly reduced (P < 0.01), with the expression of Fas and @entity185 significantly lowered but expression of @entity1379 increased slightly. CONCLUSION: RB extract could inhibit the cardiomyocyte apoptosis in animal with @entity2401 , obviously down-regulate the expression of Fas and @entity185 , and slightly up-regulate @entity1379 expression.

[Effect of radix bupleuri extract on cardiomyocyte apoptosis related genes expression in XXXX infected with coxsackie virus B3m].

@entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'Mice'] @entity185 :: ('12367(Tax:10090)', 'Gene') :: ['Caspase3'] @entity6475 :: ('MESH:D013180', 'Disease') :: ['cardiomyocytophilic strain Coxsackie'] @entity1379 :: ('12043(Tax:10090)', 'Gene') :: ['Bcl-2'] @entity2401 :: ('MESH:D009205', 'Disease') :: ['viral myocarditis']
@entity19 :: (10090,Species) :: ['mouse']
