Thioltransferase was purified 650-fold from @entity95 liver by procedures including acid treatment, heat treatment, gel filtration on Sephadex G-50, column chromatography on DEAE-cellulose, isoelectric focusing (pH 3.5-10) and gel filtration on Sephadex G-75. The final enzyme preparation was almost homogeneous in @entity2975 gel electrophoretic analysis. Only one active peak with an apparent molecular weight (Mr) of 13,000 was detected by gel filtration on Sephadex G-50 and only a single protein band with a molecular weight of 12,400 was detected by @entity3208 ( @entity214 )- @entity2975 gel electrophoresis. Isoelectric focusing revealed only one enzyme species, having an isoelectric point (pI) of 5.3. The enzyme has an optimum pH about 3.0 with @entity69171 and @entity2197 as substrates. The purified enzyme utilized some @entity1525 including @entity69171 , alpha-chymotrypsin, trypsin, bovine serum albumin, and insulin as substrates in the presence of @entity2197 . The enzyme does not act as a protein : @entity1520 isomerase (the activity of which can be measured in terms of reactivation of randomly reoxidized @entity1460 Kunitz trypsin inhibitor). The enzyme activity was inhibited by @entity2755 , but not by @entity3637 . The inhibition by @entity2755 was non-competitive (apparent K1 of 0.5 mM). Thioltransferase activity was found in the cytosol of various @entity95 tissues.

Purification and some properties of XXXX liver cytosol thioltransferase.

@entity3208 :: ('MESH:D012967', 'Chemical') :: ['sodium dodecyl sulfate'] @entity3637 :: ('MESH:D001414', 'Chemical') :: ['bacitracin'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity2197 :: ('CHEBI:16856', 'Chemical') :: ['GSH'] @entity2755 :: ('MESH:D002701', 'Chemical') :: ['chloramphenicol'] @entity69171 :: ('MESH:C011119', 'Chemical') :: ['S-sulfocysteine'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity1520 :: ('CHEBI:48343', 'Chemical') :: ['disulfide'] @entity1460 :: ('3847', 'Species') :: ['soybean'] @entity1525 :: ('MESH:D004220', 'Chemical') :: ['disulfides'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS']
@entity95 :: (9986,Species) :: ['rabbit']
