UNASSIGNED: The aim of this study was to investigate whether a novel formulation of an herbal extract, KBH-1, has an inhibitory effect on @entity28 . To determine its anti- @entity28 effects and its underlying mechanism, we performed anti- @entity28 -related experiments in vitro and in vivo. 3T3-L1 preadipocytes were analyzed for lipid accumulation as well as the protein and gene expression of molecular targets involved in @entity844 synthesis. To determine whether KBH-1 oral administration results in a reduction in high-fat diet (HFD)-induced @entity28 , we examined five groups (n = 9) of C57BL/6 @entity19 as follows: 10% kcal fat diet-fed @entity19 ( @entity11702 ), 60% kcal fat diet-fed @entity19 (HFD), HFD-fed @entity19 treated with @entity350 (tetrahydrolipstatin, marketed under the trade name Xenical), HFD-fed @entity19 treated with 150 mg/kg KBH-1 (KBH-1 150) and HFD-fed @entity19 treated with 300 mg/kg KBH-1 (KBH-1 300). During adipogenesis of 3T3-L1 cells in vitro, KBH-1 significantly reduced lipid accumulation and down-regulated the expression of master adipogenic transcription factors, including @entity16509 ( @entity19775 , @entity16509 a and @entity3229 ( @entity3229 ) y, which led to the suppression of the expression of several adipocyte-specific genes and proteins. KBH-1 also markedly phosphorylated the @entity860 -activated protein kinase (AMPK) and @entity6625 carboxylase (ACC). In addition, KBH-1-induced the inhibition effect on lipid accumulation and AMPK-mediated signal activation were decreased by blocking AMPK phosphorylation using AMPK siRNA. Furthermore, daily oral administration of KBH-1 resulted in dose-dependent decreases in body weight, fat pad mass and fat tissue size without systemic @entity137 . These results suggest that KBH-1 inhibits lipid accumulation by down-regulating the major transcription factors of the adipogenesis pathway by regulating the AMPK pathway in 3T3-L1 adipocytes and in @entity19 with HFD-induced @entity28 . These results implicate KBH-1, a safe herbal extract, as a potential anti- @entity28 therapeutic agent.

The Herbal Medicine KBH-1 Inhibits Fat Accumulation in 3T3-L1 Adipocytes and Reduces High Fat Diet-Induced XXXX through Regulation of the AMPK Pathway.

@entity350 :: ('MESH:C055122', 'Chemical') :: ['orlistat'] @entity844 :: ('CHEBI:35366', 'Chemical') :: ['fatty acid'] @entity3229 :: ('19013(Tax:10090)', 'Gene') :: ['peroxisome proliferation-activity receptor', 'PPAR'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity28 :: ('MESH:D009765', 'Disease') :: ['obesity'] @entity19775 :: ('12608(Tax:10090)', 'Gene') :: ['C/EBP) b'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity16509 :: ('12606(Tax:10090)', 'Gene') :: ['C/EBP', 'CCAAT/enhancer binding protein'] @entity11702 :: ('MESH:C537849', 'Disease') :: ['ND'] @entity860 :: ('MESH:D000249', 'Chemical') :: ['adenosine monophosphate'] @entity6625 :: ('MESH:D000105', 'Chemical') :: ['acetyl-CoA']
@entity28 :: (MESH:D009765,Disease) :: ['Obesity']
