Regulation of cell proliferation by protein @entity669 phosphatases ( @entity48252 ) suggests that @entity48252 are important @entity5 suppressor genes. The gene encoding the leukocyte common-antigen-related ( @entity18642 ) PTP receptor maps to chromosome 1p32-33, a region in which loss of heterozygosity is associated with @entity1 @entity1911 and other @entity4969 . The @entity35 @entity1911 PC12 cell line was originally derived from the transplantable P259 @entity5 originating from the New England Deaconess Hospital (NEDH) line of @entity35 inbred @entity35 . Compared with their @entity35 counterparts, 1-2-year-old NEDH @entity35 exhibit a high incidence of spontaneous @entity1911 . This study investigates whether levels of @entity18642 transcripts and protein are altered in NEDH adrenal tissue prior to @entity5 onset. In addition, alternative splicing of an @entity18642 extracellular domain [ @entity18642 alternatively spliced element-c (LASE-c)], regulating @entity18642 interaction with extracellular matrix components, was examined. These changes in @entity18642 expression and alternative splicing were hypothesized to be more pronounced in @entity5 tissue and PC12 cells. Northern blot analysis demonstrated the presence of the approximately 5 kb @entity18642 transcript in all cell lines examined, except PC12. In adrenal medulla tissue harvested from 2-3-month-old @entity35 , @entity18642 approximately 8 and approximately 5 kb transcript expression was decreased in NEDH compared with @entity35 samples. RT-PCR demonstrated increased splicing of the LASE-c 27 bp alternatively spliced insert in the @entity18642 extracellular domain in NEDH adrenal medulla tissue. Even greater LASE-c splicing was detected in adrenal medulla @entity5 tissue derived from 12-month-old NEDH @entity35 and in PC12 cells. Western blot analysis demonstrated decreased levels of @entity18642 protein and increased levels of LASE-c containing @entity18642 protein isoforms in NEDH adrenal medulla tissue. These studies demonstrate that patterns of altered @entity18642 expression present in PC12 cells and in @entity5 tissue are also present in adrenal tissue predisposed to a high incidence of spontaneous @entity1911 .

Leukocyte common-antigen-related XXXX phosphatase receptor: altered expression of mRNA and protein in the New England Deaconess Hospital @entity35 line exhibiting spontaneous @entity1911 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity1911 :: ('MESH:D010673', 'Disease') :: ['pheochromocytoma', 'pheochromocytomas'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'pheochromocytoma tumor'] @entity4969 :: ('MESH:D017599', 'Disease') :: ['neuroectodermal tumors'] @entity18642 :: ('360406(Tax:10116)', 'Gene') :: ['LAR'] @entity48252 :: ('29498(Tax:10116)', 'Gene') :: ['PTPs'] @entity35 :: ('10116', 'Species') :: ['rat', 'Wistar', 'rats'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine']
@entity669 :: (CHEBI:18186,Chemical) :: ['tyrosine']
