UNASSIGNED: The @entity1708 translocases (ANTs) play a vital role in energy metabolism via @entity857 / @entity855 exchange in eukaryotic cells. @entity20874 (Echinodermata: Holothuroidea) is an important economic species in China. Here, a cDNA representing an ANT gene of A. japonicus was isolated and characterized from respiratory tree and named AjANT. The full-length AjANT cDNA is 1924  bp, including a 5'-untranslated region (UTR) of 38  bp, 3'-UTR of 980  bp and an open reading frame (ORF) of 906  bp encoding a polypeptide of 301 @entity3534 . The protein contains three homologous repeat Mito_carr domains (Pfam00153). The deduced AjANT protein sequence has 49-81% in comparison to ANT proteins from other individuals. The predicted tertiary structure of AjANT protein is highly similar to animal ANT proteins. Phylogenetic analysis showed that the AjANT is closely related to Holothuroidea ANT genes. Real-time quantitative reverse transcription-PCR (qPCR) analysis showed that AjANT expression is higher in the respiratory tree than in other examined tissues. After thermal stress or LPS challenge, expression of AjANT was significantly fluctuant compared to the control. These results suggested that changes in the expression of ANT gene might be involved in immune defense and in protecting A.  japonicus against thermal stress.

An XXXX translocase (ANT) gene from @entity20874 ; molecular cloning and expression analysis in response to lipopolysaccharide (LPS) challenge and thermal stress.

@entity1708 :: ('CHEBI:61293', 'Chemical') :: ['adenine nucleotide'] @entity3534 :: ('MESH:D000596', 'Chemical') :: ['amino acids'] @entity20874 :: ('307972', 'Species') :: ['Apostichopus japonicus'] @entity857 :: ('MESH:D000244', 'Chemical') :: ['ADP'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity1708 :: (CHEBI:61293,Chemical) :: ['adenine nucleotide']
