BACKGROUND/AIM: In @entity1593 ( @entity1593 ), there is increased mRNA coding for @entity8265 ( @entity8265 ) and @entity686 . In seronegative celiac @entity1 , the mucosal immune complexes anti- @entity8265 IgA/ @entity8265 are found. We assayed @entity8265 - and @entity379 -mRNA in the mucosa of @entity1 with a clinical suspicion of seronegative @entity1593 and correlated the values with intraepithelial CD3 lymphocytes (IELs). MATERIALS AND METHODS: Distal duodenum specimens from 67 @entity1 were retrieved and re-evaluated for immunohistochemically proven CD3 IELs. Five 10  m sections were used for the extraction and assay of @entity8265 and @entity379 coding mRNA levels using reverse transcriptase real-time polymerase chain reaction (RT-PCR). Samples from 15 seropositive @entity1593 @entity1 and 15 healthy subjects were used as positive and negative controls. Results were expressed as fold-change. RESULTS: Our series was divided into three groups based on IEL count: >25 (14 @entity1 : group A), 15-25 (26 @entity1 : group B), and 0-15 (27 @entity1 : Group C). @entity8265 -mRNA levels were (mean    SD): @entity1593 = 9.8    2.6; group A = 10.04    4.7; group B = 4.99    2.3; group C = 2.26    0.8, controls = 1.04    0.2 ( @entity1593 = group A > group B > group C = controls). @entity379 -mRNA levels were: @entity1593 = 13.4    3.6; group A = 7.28    3.6; group B = 4.45    2.9; group C = 2.06    1.21, controls = 1.04    0.4. CONCLUSIONS: Our results suggest that @entity8265 - and IFNymRNA levels are increased in both seropositive and potential seronegative @entity1 with @entity1593 , showing a strong correlation with the CD3 IEL count at stage Marsh 1. An increase in both molecules is found even when IELs are in the range 15-25 (Marsh 0), suggesting the possibility of a "gray zone" inhabited by @entity1 which should be closely followed up in @entity74 .

Mucosal molecular pattern of XXXX and @entity379 in suspected seronegative @entity1593 at marsh 1 and 0 stages.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity1593 :: ('MESH:D002446', 'Disease') :: ['celiac disease', 'CD'] @entity686 :: ('3438', 'Gene') :: ['interferon gamma (IFNa)'] @entity379 :: ('3458', 'Gene') :: ['IFNa'] @entity74 :: ('MESH:D030342', 'Disease') :: ['gluten-related disorders'] @entity8265 :: ('7052', 'Gene') :: ['tissue transglutaminase', 'tTG']
@entity8265 :: (7052,Gene) :: ['tissue transglutaminase']
