CONTEXT: Beta-cells have a limited replicative capacity; hence, there is always a quest for sources of islet regeneration to compensate for the loss of functional beta-cells in @entity6 . OBJECTIVE: To test the hypothesis of whether intra islet precursor cells of islets isolated from a @entity6 pancreas and in vitro @entity3839 treated islets are capable of giving rise to neoislets. INTERVENTIONS: @entity3839 treatment was given to @entity19 and to islets isolated from normal @entity19 . Islets were isolated from @entity6 @entity19 , cultured on matrigel coated plates with a well-defined serum free medium containing mitotic ( @entity4571 ) and differentiating (keratinocyte growth factor) agents. Initially, islets gave rise to an epithelial-like cell monolayer and, later on, differentiated into islet-like clusters. These were characterized for the ductal epithelial cell specific markers @entity25634 and @entity20452 and for the islet specific markers-insulin and @entity15980 . Insulin secretion in response to @entity413 and @entity534 was estimated by ELISA. RESULTS: A @entity25634 and @entity20452 positive precursor cell population was found scattered throughout the epithelial monolayer. Upon addition of the keratinocyte growth factor, these precursor cells gave rise to islet-like clusters which were confirmed to be islets by marker studies. Though @entity3839 treatment on islets of normal @entity19 allowed proliferation of the epithelial monolayer, it did not give rise to neoislets under similar growth conditions. CONCLUSION: The present study reveals that @entity3839 treatment of normal islets in vitro leads to the loss of the potential of intra islet precursor cells to form neoislets; however, in @entity3839 -induced experimental @entity6 , they retain their potential to generate new islets opening a novel putative way of treating @entity6 .

Islet generation from intra islet precursor cells of XXXX pancreas: in vitro studies depicting in vivo differentiation.

@entity6 :: ('MESH:D003920', 'Disease') :: ['diabetic', 'diabetes'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity3839 :: ('MESH:D013311', 'Chemical') :: ['Streptozotocin', 'streptozotocin'] @entity534 :: ('CHEBI:16467', 'Chemical') :: ['L-arginine'] @entity15980 :: ('18609(Tax:10090)', 'Gene') :: ['PDX1'] @entity25634 :: ('16669(Tax:10090)', 'Gene') :: ['cytokeratin-19'] @entity4571 :: ('MESH:D009536', 'Chemical') :: ['nicotinamide'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity20452 :: ('110310(Tax:10090)', 'Gene') :: ['cytokeratin-7']
@entity6 :: (MESH:D003920,Disease) :: ['diabetic']
