@entity1420 pretreatment increases the number of synchronously proliferating stromal cells in the ovariectomized @entity35 uterus, but @entity172 is necessary to stimulate reentry into the cell cycle. To investigate the mechanisms underlying differential hormone actions, sexually mature ovariectomized @entity35 were injected with @entity1420 (2 mg) for three consecutive days. @entity53 17-beta (0.6 microg) was administered to initiate cell proliferation. Uterine samples were collected at timed intervals. Cell entry into DNA replication was monitored by injecting @entity1603 (1 mg/100 g body weight) 2 h before necropsy. Demicolchicine (400 microg) was injected 30 min before necropsy to assess transit into M phase. Temporal progress through G1 was determined by spatial changes in @entity69233 proteins. Total @entity69233 protein and mRNA was measured by Western and Northern blotting. @entity172 increased the number of @entity1603 -positive stromal cells (P < 0.05), compared with the number in @entity35 treated with @entity1420 alone. An increase (P < 0.05) in the number of M-phase cells occurred at 12 h post @entity172 . There was no evidence for epithelial cell proliferation in response to @entity134 treatments. @entity12003 mRNA was expressed in the uteri of ovariectomized and hormone treated @entity35 . The D-type cyclin proteins, however, were not evident in stromal cells without @entity172 treatment. @entity1420 pretreatment inhibited @entity172 -dependent epithelial cell proliferation while redirecting D-type cyclin expression to the uterine stroma. Stromal cell transit through G1 required nongenomic @entity134 -dependent action on signal transduction pathways that control the nuclear localization and cell type-specific expression of the D-type cyclin proteins.

Transit of XXXX uterine stromal cells through G1 phase of the cell cycle requires temporal and cell-specific hormone-dependent changes on cell cycle regulators.

@entity172 :: ('MESH:D004967', 'Chemical') :: ['estrogen', 'Estrogen'] @entity1603 :: ('MESH:D001973', 'Chemical') :: ["5-bromo-2'-deoxyuridine"] @entity1420 :: ('MESH:D011374', 'Chemical') :: ['progesterone', 'Progesterone'] @entity69233 :: ('58919;25193', 'Gene') :: ['cyclin D1/D3'] @entity12003 :: ('58919(Tax:10116)', 'Gene') :: ['Cyclin D1/D3'] @entity53 :: ('MESH:D004958', 'Chemical') :: ['Estradiol'] @entity134 :: ('CHEBI:35341', 'Chemical') :: ['steroid'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats']
@entity35 :: (10116,Species) :: ['rat']
