UNASSIGNED: Numerous studies have found that @entity969 ( @entity27489 ) metabolism plays a crucial role in the environmental stress response of many plant species. The majority of @entity27489 metabolism-associated reports refer to acid invertases (Ac-Invs). However, alkaline/neutral Invs (A/N-Invs) have been poorly studied. In this study, a @entity3505 A/N-Inv gene, Ta-A/N- @entity68302 , with three copies located on chromosomes 4A, 4B, and 4D, was cloned from a @entity3505 -Puccinia striiformis @entity10327 . tritici (Pst) interaction cDNA library. Transcripts of the three Ta-A/N- @entity68302 copies were up-regulated in @entity3505 leaves that were infected by Pst or had experienced certain abiotic treatments. Furthermore, the expression of Ta-A/N- @entity68302 was decreased by treatment with exogenous hormones. Heterologous mutant complementation and subcellular localization revealed that Ta-A/N- @entity68302 is a cytoplasmic invertase. Knocking down all three copies of Ta-A/N- @entity68302 using the @entity18084 -induced gene silencing system reduced the susceptibility of @entity3505 to the Pst virulent pathotype CYR31, which is associated with pathogen-induced @entity5568 accumulation and enhanced @entity39 . Interestingly, 48h dark treatment of the Ta-A/N- @entity68302 -knockdown plants immediately after inoculation abrogated their enhanced resistance, suggesting that @entity5568 production and its associated cell death and resistance in the Ta-A/N- @entity68302 -silenced plants require light. Consistent with this observation, photosynthesis and reactive @entity26 species (ROS)-related genes were significantly up-regulated in the Ta-A/N- @entity68302 -knockdown plants infected by CYR31 under light exposure. These results suggest that Ta-A/N- @entity68302 might act as a negative regulator in @entity3505 disease resistance to Pst by increasing cytoplasmic hexose accumulation and downregulating photosynthesis of the leaves to avoid cell death due to excessive ROS production.

Down-regulation of a XXXX alkaline/neutral invertase correlates with reduced host susceptibility to @entity3505 stripe rust caused by Puccinia striiformis.

@entity5568 :: ('D006861', 'Chemical') :: ['H2O2'] @entity27489 :: ('CHEBI:37952', 'Chemical') :: ['Suc'] @entity10327 :: ('239', 'Species') :: ['f. sp'] @entity68302 :: ('542982(Tax:4565)', 'Gene') :: ['Inv1'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity18084 :: ('12327', 'Species') :: ['barley stripe mosaic virus'] @entity3505 :: ('4565', 'Species') :: ['wheat']
@entity3505 :: (4565,Species) :: ['wheat']
