@entity794 is the bacterial pathogen which is responsible for approximately 80% of all cases of @entity1 @entity4034 . It can invade and remain within osteoblasts. The fate of intracellular Staph. aureus after the death of the osteoblast has not been documented. We exposed @entity1 osteoblasts to Staph. aureus. After @entity281 , the osteoblasts were either lysed with Triton X-100 or trypsinised. The bacteria released from both the trypsinised and lysed osteoblasts were cultured and counted. Colonies of the recovered bacteria were then introduced to additional cultures of @entity1 osteoblasts. The number of intracellular Staph. aureus recovered from the two techniques was equivalent. Staph. aureus recovered from time zero and 24 hours after @entity281 , followed by lysis/trypsinisation, were capable of invading a second culture of @entity1 osteoblasts. Our findings indicate that dead or dying osteoblasts are capable of releasing viable Staph. aureus and that Staph. aureus released from dying or dead osteoblasts is capable of reinfecting @entity1 osteoblasts in culture.

Intracellular XXXX . A mechanism for the indolence of @entity4034 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity4034 :: ('MESH:D010019', 'Disease') :: ['osteomyelitis'] @entity794 :: ('1280', 'Species') :: ['Staphylococcus aureus']
@entity794 :: (1280,Species) :: ['Staphylococcus aureus']
