External radiation seems to be associated with increased amounts of cytokines and other cellular modulators. @entity73 and @entity82 are examples of typical long term damage caused by radiotherapy. Adipose tissue-derived stem cells (ASC) are discussed to enhance wound healing, but their role in wounds due to radiotherapy is poorly understood. Normal @entity1 fibroblasts (NHF) and ASCs were co-cultured and external radiation with doses from 2-12 Gray (Gy) was delivered. Cell proliferation and mRNA levels of matrix metalloproteinases ( @entity3334 , @entity1476 and @entity1550 ) were determined 48 h after irradiation of the co-cultures by qPCR. Additionally, tissue inhibitors of matrix metalloproteinases ( @entity2843 , @entity1552 ) were determined by enzyme-linked immunosorbent assay (ELISA). There was a reduction of cell proliferation after external radiation in mono-cultures of NHFs and ASCs compared to controls without irradiation. The co-culture of ASCs and NHFs showed reduced impairment of cell proliferation after external radiation. Gene expression of @entity3334 and @entity1550 was reduced after external irradiation in NHF. @entity1476 expression of irradiated NHFs was increased. In the co-culture setting, @entity3334 and @entity1476 gene expression levels were upregulated. @entity2843 and @entity1552 protein expression was increased after irradiation in NHFs and their co-cultures with ASCs. ASCs seem to stimulate cell proliferation of NHFs and modulate relevant soluble mediators as well as proteinases after external radiation.

A Co-Culture Model of Fibroblasts and Adipose Tissue-Derived Stem Cells Reveals New Insights into XXXX After Radiotherapy.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1552 :: ('7077', 'Gene') :: ['TIMP2'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis'] @entity3334 :: ('4312', 'Gene') :: ['MMP1'] @entity1476 :: ('4313', 'Gene') :: ['MMP2'] @entity73 :: ('MESH:D003072', 'Disease') :: ['Impaired microcirculation'] @entity1550 :: ('4322', 'Gene') :: ['MMP13'] @entity2843 :: ('7076', 'Gene') :: ['TIMP1']
@entity73 :: (MESH:D003072,Disease) :: ['Impaired Wound Healing']
