Many cellular damages either in normal or cancerous tissues are the outcome of molecular events affected by ionizing radiation. T-cells are the most important among immune system agents and are used for biological radiation dose measurement in recommended standard methods. The herbs with immune modulating properties may be useful to reduce the risk of the damages and subsequently the diseases. The T-cells as the most important immune cells being targeted for biological dosimetry of radiation. This study proposes a flowcytometric-method based on @entity2822 - and @entity2667 (PI)-labeled @entity4404 to assess apoptosis in blood T-cells after irradiation in both presence and absence of fenugreek extract. T-cells peripheral blood lymphocyte isolated from blood samples of healthy individuals with no irradiated job background. The media of cultured cells was irradiated 1-h after the fenugreek extract was added. The number of apoptotic cells was assessed by @entity4404 protocol and multicolor flowcytometry. An obvious variation in apoptotic cells number was observed in presence of fenugreek extract (>80%). The results suggest that fenugreek extract can potentiate the radiation induced apoptosis or @entity137 in blood T-cells (P < 0.05).

The Effects of Fenugreek on Radiation Induced XXXX for Human Blood T-Cells in Radiotherapy.

@entity4404 :: ('308', 'Gene') :: ['annexin-V'] @entity137 :: ('MESH:D064420', 'Disease') :: ['radiation toxicity'] @entity2822 :: ('CHEBI:37926', 'Chemical') :: ['fluorescein isothiocyanate'] @entity2667 :: ('MESH:D011419', 'Chemical') :: ['propidium iodide']
@entity137 :: (MESH:D064420,Disease) :: ['Toxicity']
