OBJECTIVE: To investigate in situ visualization using near-infrared quantum dots (QDs) conjugated with @entity369 - @entity2228 - @entity2574 (ROD) peptide fluorescent probes in @entity957 (08CC). METHODS: QDs with emission wavelength of 800 nm (QD800) were conjugated with RGD peptides to produce QD800-RGD fluorescent probes. @entity1 OSCC cell line BcaCD885 was inoculated in @entity19 cheeks to establish OSCC @entity19 models. Frozen BcaCD885 @entity5 slices were immunofluorescence double stained by using QD800-RGD and @entity31973 monoclonal antibody and were observed using a laser scanning confocal microscope. QD800-RGD was injected into the OSCC models through the tail veins, and the in situ visualization was analyzed at different time points. The @entity19 were sacrificed 12 h after injection to isolate @entity5 for the ex vivo analysis of probe localization in the @entity5 . RESULTS: QD800-RGD specifically targeted the integrin avb3 expressed in the endothelial cells of @entity5 angiogenic vessels in vitro and in vivo, producing clear @entity5 fluorescence images after intravenous injection. The most complete @entity5 images with maximal signal-to-noise ratios were observed 0.5 h to 6 h after injection of the probe and significantly reduced 9 h after the injection. However, the @entity5 image was still clearly visible at 12 h. CONCLUSION: Using intravenously injected QD800-RGD generates high quality OSCC images when integrin avb3, which is expressed in the endothelial cells of @entity5 angiogenic vessels, is used as the target. The technique offers great potential in the diagnosis and individual treatment of OSCC.

[In situ visual imaging of @entity957 in XXXX by using near-infrared quantum dots conjugated with @entity369 - @entity2228 - @entity2574 peptide fluorescent probes].

@entity1 :: ('9606', 'Species') :: ['Human'] @entity2228 :: ('CHEBI:57305', 'Chemical') :: ['glycine'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'nude mice'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity957 :: ('MESH:D002294', 'Disease') :: ['oral squamous cell carcinoma'] @entity31973 :: ('13805(Tax:10090)', 'Gene') :: ['CD105'] @entity2574 :: ('CHEBI:22660', 'Chemical') :: ['aspartic acid'] @entity369 :: ('CHEBI:29016', 'Chemical') :: ['arginine']
@entity19 :: (10090,Species) :: ['mice']
