We examined the effects of @entity1499 ( @entity1500 ) on the level of @entity842 (NO) produced by peritoneal macrophages from @entity35 with @entity1753 ( @entity1753 ) ex vivo. During the development of @entity1753 , paw swelling increased and @entity558 enhanced the capacity of peritoneal macrophages to produce NO and @entity1421 ( @entity1421 ). @entity1500 (0.1 mg/kg, p.o.) treatment for 21 days reduced the paw swelling, and inhibited the increased NO and @entity1421 production. However, when @entity1500 (0.1 mg/kg, p.o.) was administered to @entity35 with established @entity1753 , these parameters were not significantly influenced. In normal @entity35 , @entity1500 (0.1 mg/kg, p.o.) treatment for 21 days did not change NO and @entity1421 production of @entity558 -stimulated macrophages. On the other hand, macrophages from normal and @entity1753 @entity35 cultured in the presence of @entity1500 (1, 10 and 100 microM), were activated by @entity558 in vitro. @entity1500 did not influence NO or @entity1421 production by @entity558 -stimulated macrophages in normal and @entity1753 @entity35 . By contrast, @entity3352 (IM) (1.0 mg/kg, p.o.) treatment for 21 days reduced the paw swelling, and inhibited NO and @entity1421 production in @entity1753 @entity35 . IM inhibited significantly @entity1421 production, but did not influence NO production by @entity558 -stimulated macrophages in vitro. These results suggest that @entity1500 treatment reduces NO production in peritoneal macrophages in @entity1753 @entity35 , and these actions of @entity1500 may have an inhibitory effect without the modulation of @entity1421 .

@entity1499 suppresses @entity842 production ex vivo in macrophages from @entity35 with XXXX .

@entity558 :: ('MESH:C536528', 'Disease') :: ['LPS'] @entity1421 :: ('MESH:D015232', 'Chemical') :: ['PGE2', 'prostaglandin E2'] @entity1753 :: ('MESH:D001169', 'Disease') :: ['adjuvant-induced arthritis', 'AA'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity1500 :: ('CHEBI:44185', 'Chemical') :: ['MTX'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity3352 :: ('MESH:D007213', 'Chemical') :: ['indomethacin'] @entity1499 :: ('MESH:D008727', 'Chemical') :: ['methotrexate']
@entity1753 :: (MESH:D001169,Disease) :: ['adjuvant-induced arthritis']
