We have cloned and characterized a cDNA from @entity958 that most likely encodes a novel member of the vast superfamily of G-protein-coupled receptor proteins (GPCRs). By taking advantage of @entity535 sequence similarities between plant expressed sequence tags (ESTs) and established G-protein-coupled receptor sequences, a probe was obtained which was used for the screening of an @entity958 cDNA library. The cDNA which was found is very infrequently represented in the cDNA library, suggesting a low and/or spatially restricted expression. A region of the translated sequence of the cDNA shows the highest similarity to @entity268 receptors from the slime mold @entity4328 . The same region is also similar to that in members of the animal calcitonin family of receptors. Another region of the putative receptor, however, is similar to sequences of @entity750 receptors and other receptors of the so-called rhodopsin family of GPCRs. The rhodopsin family has numerous members in higher vertebrate species. Alignments and phylogenetic analyses of the regions of similarity yielded results in accordance with other evolutionary considerations. Our cDNA thus occurred on a distinct major branch in relation to the rest of the rhodopsin family. In relation to the calcitonin family, our cDNA and @entity268 receptors occurred together on a distinct major branch but appear to have diverged from each other shortly after their divergence from the rest of the calcitonin family. Other features further argue for a tentative identification of it as a GPCR. It displays seven discrete and strongly predicted transmembrane domains when analyzed in hydropathy plots. The preferred orientation is with the @entity1472 terminus towards the outside. It has one @entity2569 residue in extracellular loop 1 and another in extracellular loop 2. @entity2569 residues in these loops are known to form @entity1520 bridges in many other GPCRs. Finally, it has several fully conserved @entity1522 that belong to the most conserved in previously known GPCRs, that occur in the above regions of similarity.

Cloning of a putative G-protein-coupled receptor from XXXX .

@entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity1472 :: ('CHEBI:46882', 'Chemical') :: ['amino'] @entity958 :: ('3702', 'Species') :: ['Arabidopsis thaliana', 'Arabidopsis'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity2569 :: ('CHEBI:17561', 'Chemical') :: ['Cys'] @entity4328 :: ('44689', 'Species') :: ['Dictyostelium discoideum'] @entity750 :: ('MESH:D012701', 'Chemical') :: ['serotonin'] @entity1520 :: ('CHEBI:48343', 'Chemical') :: ['disulfide']
@entity958 :: (3702,Species) :: ['Arabidopsis thaliana']
