@entity42614 ( @entity35777 ) is one of the main components of traditional Chinese medicine plant Erigeron breviscapus (Vant.) Hand.-Mazz. In this paper, we studied the protective effects of @entity35777 on @entity1 cardiac microvascular endothelial cells (HCMECs) against @entity519 and its possible target-related proteins. Results of @entity98 assay showed that pretreatment of @entity35777 at doses of 1, 5, and 10    M for 2   h could significantly inhibit the decrease in cell viability of HCMECs induced by @entity519 . Subcellular fractions of cells treated with vehicle control, 1    M @entity35777 , @entity519 , or 1    M @entity35777 + @entity519 were separated by ultracentrifugation. The protein expression profiles of cytoplasm and membrane/nuclei fractions were checked using protein two-dimensional electrophoresis (2-DE). Proteins differentially expressed between control and @entity35777 -treated group, control and HR group, or HR and @entity35777 + HR group were identified using mass spectrometry ( @entity15 / @entity15 ). Possible interaction network of these target-related proteins was predicted using bioinformatic analysis. The influence of @entity35777 on the expression levels of these proteins was confirmed using Western blotting assay. The results indicated that proteins such as @entity31074 protein ( @entity31074 ), @entity1457 60   kDa protein 1 ( @entity11217 ), and @entity57550 ( @entity57550 ) might play important roles in the effects of @entity35777 .

Protective Effects of @entity42614 on @entity1 Cardiac Microvascular Endothelial Cells against XXXX -Reoxygenation Injury and Its Possible Target-Related Proteins.

@entity1 :: ('9606', 'Species') :: ['human'] @entity42614 :: ('MESH:C484876', 'Chemical') :: ['Scutellarin'] @entity35777 :: ('CHEBI:51110', 'Chemical') :: ['SCU'] @entity1457 :: ('MESH:D012769', 'Disease') :: ['heat shock'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity519 :: ('MESH:D000860', 'Disease') :: ['hypoxia-reoxygenation (HR) injury', 'HR injury'] @entity57550 :: ('908', 'Gene') :: ['chaperonin containing TCP1 subunit 6A isoform', 'CCT6A'] @entity11217 :: ('3329', 'Gene') :: ['HSPD1'] @entity15 :: ('MESH:D009103', 'Disease') :: ['MS'] @entity31074 :: ('3692', 'Gene') :: ['p27BBP', 'EIF6']
@entity519 :: (MESH:D000860,Disease) :: ['Hypoxia']
