Despite the recognised role of the @entity10124 ( @entity7050 ) in the encapsulation and release of proteins from @entity7050 microspheres, the importance that the characteristics of the protein have in these processes has not yet been sufficiently investigated. The aim of this work was to study the simultaneous effect of the protein and @entity7050 properties and of the microencapsulation process on the physicochemical and in vitro release characteristics of protein-loaded @entity7050 microspheres. Two model proteins of different isoelectric points (pI), bovine serum albumin (BSA) (pI = 4.6) and @entity16977 ( @entity16977 ) (pI = 11.2), and two different molecular weights (Mw) of @entity7050 were selected. Microspheres were prepared using the water-in-oil-in-water (w/o/w) solvent extraction and the oil-in-oil (o/o) solvent evaporation techniques. Results showed that BSA was efficiently encapsulated independent of the @entity7050 Mw, whereas the encapsulation of @entity16977 was favoured with the low Mw @entity7050 . The co-encapsulation of a surfactant ( @entity12185 or 331) reduced the protein encapsulation efficiency, especially of BSA. These results suggested that the tensoactive properties of the protein and its affinity for the @entity7050 are major determinants of the protein encapsulation. Both proteins released faster from the microspheres prepared by the o/o solvent evaporation procedure, with respect to those prepared by the w/o/w solvent extraction technique. In addition, both polymer Mw and protein type had an effect on the protein release rate. The release rate of both proteins, in the absence of a surfactant, was faster from the low Mw @entity7050 microspheres. However, the release rate constant was higher for BSA than for @entity16977 irrespective of the @entity7050 Mw. In addition, the co-encapsulation of a surfactant led, in most cases, to a faster release of the encapsulated protein. To conclude, these results suggest that protein release from @entity7050 microspheres is not only governed by the @entity7050 erosion rate and protein diffusion through the water-filled channels, but is highly affected by the protein properties and its possible interaction with @entity7050 and its degradation products.

Protein encapsulation and release from XXXX microspheres: effect of the protein and polymer properties and of the co-encapsulation of surfactants.

@entity7050 :: ('MESH:C043435', 'Chemical') :: ['PLGA'] @entity16977 :: ('4069', 'Gene') :: ['lysozyme', 'LZM'] @entity10124 :: ('CHEBI:53493', 'Chemical') :: ['poly(lactide-co-glycolide)'] @entity12185 :: ('MESH:D020442', 'Chemical') :: ['poloxamer 188']
@entity10124 :: (CHEBI:53493,Chemical) :: ['poly(lactide-co-glycolide)']
