Identification and characterization of @entity5 stem cells (CSCs) in @entity356 are difficult owing to the lack of specific markers and consensus methods. In this study, we show that cells with the @entity5399 surface marker in @entity356 could be enriched under non-adherent, serum-free and sphere-forming conditions. These @entity5399 (+) cells possess a higher ability to initiate @entity5 in vivo and could re-establish the cellular hierarchy of @entity5 from single-cell implantation, demonstrating their self-renewal properties. Interestingly, higher proportion of @entity5399 (+) cells correlates with higher in vivo tumorigenicity of gastric @entity5 models. In addition, it was found that @entity1722 was overexpressed in about 25% of the gastric @entity5 models, which correlates with the higher level of @entity5399 expression in these @entity5 . Trastuzumab (humanized anti- @entity1722 antibody) treatment of high-tumorigenic gastric @entity5 models could reduce the @entity5399 (+) population in @entity5 mass and suppress @entity5 growth when combined with traditional chemotherapy. Moreover, tumorigenicity of @entity5 cells could also be suppressed when trastuzumab treatment starts at the same time as cell implantation. Therefore, we have identified a CSC population in gastric @entity5 characterized by their @entity5399 phenotype. The finding that trastuzumab targets the CSC population in @entity356 suggests that @entity1722 signaling has a role in maintaining CSC populations, thus contributing to carcinogenesis and @entity5 invasion. In conclusion, the results from this study provide new insights into the gastric tumorigenic process and offer potential implications for the development of anticancer drugs as well as therapeutic treatment of @entity356 .

Trastuzumab (herceptin) targets XXXX stem cells characterized by @entity5399 phenotype.

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'primary tumors', 'cancer', 'primary tumor', 'tumors'] @entity5399 :: ('7070', 'Gene') :: ['CD90'] @entity356 :: ('MESH:D013274', 'Disease') :: ['gastric tumors', 'gastric cancer', 'gastric cancers'] @entity1722 :: ('2064', 'Gene') :: ['ERBB2']
@entity356 :: (MESH:D013274,Disease) :: ['gastric cancer']
