UNASSIGNED: The most life-threatening aspect of @entity5 is @entity3 ; @entity5 @entity1 mortality is mainly due to @entity3 . Among all metastases, presence of brain @entity3 is one with the poorest prognosis; the median survival time can be counted in months. Therefore, prevention or decreasing their incidence would be highly desired both by @entity1 and physicians. Metastatic cells invading the brain must breach the cerebral vasculature, primarily the blood-brain barrier. The key step in this process is the establishment of firm adhesion between the @entity5 cell and the cerebral endothelial layer. Using the atomic force microscope, a high-resolution force spectrograph, our aim was to explore the connections among the cell morphology, cellular mechanics, and biological function in the process of transendothelial migration of metastatic @entity5 cells. By immobilization of a @entity320 cell to an atomic force microscope's cantilever, intercellular adhesion was directly measured at quasi-physiological conditions. Hereby, we present our latest results by using this @entity320 -decorated probe. Binding characteristics to a confluent layer of brain endothelial cells was directly measured by means of single-cell force spectroscopy. Adhesion dynamics and strength were characterized, and we present data about spatial distribution of elasticity and detachment strength. These results highlight the importance of cellular mechanics in brain @entity3 formation and emphasize the enormous potential toward exploration of intercellular dynamic-related processes.

Direct mapping of XXXX cell - endothelial cell interactions.

@entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis']
@entity320 :: (MESH:D008545,Disease) :: ['melanoma']
