OBJECTIVE: To investigate @entity6703 ( @entity6703 ) samples retrieved in @entity2099 by single insertion of the needle, for rapid assessment of chromosome copy number, zygosity, and cross-contamination between fetuses, using Quantitative Fluorescent Polymerase Chain Reaction (QF-PCR) amplification of highly polymorphic microsatellite markers. METHODS: Fifty-two @entity2099 were selected (47 twins, 5 triplets) and 108 samples of @entity6703 were sampled between 12 to 20 weeks of gestation (mean 15.5) using the single-needle technique. Aneuploidy screening by QF-PCR amplification of short tandem repeats (STRs) on chromosomes X, Y, 21, 13, and 18 was carried out within 24 h of collection. Owing to the sampling procedure, the eventual presence of contamination between fetuses was also evaluated in every case. RESULTS: Normal and aneuploid fetuses were readily identified by QF-PCR. Fetal reduction was made available, for trisomic fetuses, without further waiting for completion of fetal karyotyping. In twin gestations, the ultrasound examination of chorionicity was always in agreement with the molecular assessment of zygosity. Contamination between fetuses due to the sampling procedure with a single puncture was never observed. CONCLUSION: Rapid prenatal diagnosis of @entity2007 by QF-PCR is a sensitive, efficient, and reliable assay. When applied in @entity2099 , it has the added value of allowing the assessment of zygosity in all cases, independently of chorionicity and fetal sex.

Rapid prenatal diagnosis of @entity2007 and zygosity in XXXX by amniocentesis with single insertion of the needle and quantitative fluorescent PCR.

@entity2007 :: ('MESH:D000782', 'Disease') :: ['aneuploidies'] @entity2099 :: ('MESH:D011254', 'Disease') :: ['multiple pregnancies'] @entity6703 :: ('MESH:D004619', 'Disease') :: ['AF', 'amniotic fluid']
@entity2099 :: (MESH:D011254,Disease) :: ['multiple pregnancies']
