@entity671 receptor ( @entity15304 ) has been established to be essential for hearing. However, the expression of @entity15304 in the cochlea in the period of auditory development remains unknown. We investigated the expression of @entity15304 in the developing @entity35 cochlea using immunohistochemistry and real-time reverse transcription polymerase chain reaction (RT-PCR). We observed its presence in the developing @entity35 cochlea, and changes in @entity15304 protein expressions from the early post-natal period to adult. At birth (post-natal day 0, P0), @entity15304 expression was only found in Hensen's cell. @entity15304 immunoreactivity first appeared in the sensory hair cells in the organ of Corti at P2. This localization was confirmed by means of double-labeling experiments with @entity52630 , a marker for cochlear hair cells. Colocalization of @entity15304 and @entity52630 from P2 to the second post-natal week suggested early expression of @entity15304 in developing inner and outer hair cells. Claudius' cells near the spiral ligament were labelled for @entity15304 from P8 onwards. Transient @entity15304 expression was observed in the stria vascularis in early post-natal @entity35 from P4 to P8. @entity4494 neurons also exhibited weaker @entity15304 fluorescence signals during post-natal development. The results of RT-PCR demonstrated that all three @entity15304 isoforms ( @entity15304 , @entity62044 , and @entity9423 ) were present in @entity35 cochlea during four different developmental stages of cochlea, from P0 to @entity12994 . Present immunohistochemical evidence for both change and maintenance of expression of @entity15304 during post-natal development of the @entity35 cochlea indicated the possible involvement of @entity15304 -mediated @entity460 signaling in cochlear development.

Developmental expression of @entity671 receptor in the post-natal XXXX cochlea.

@entity671 :: ('MESH:D015544', 'Chemical') :: ['Inositol 1, 4, 5-trisphosphate'] @entity62044 :: ('81678(Tax:10116)', 'Gene') :: ['IP3R2'] @entity9423 :: ('25679(Tax:10116)', 'Gene') :: ['IP3R3'] @entity4494 :: ('MESH:D045888', 'Disease') :: ['Spiral ganglion'] @entity15304 :: ('25262(Tax:10116)', 'Gene') :: ['IP3R', 'IP3R1'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity12994 :: ('94189(Tax:10116)', 'Gene') :: ['P28'] @entity52630 :: ('266714(Tax:10116)', 'Gene') :: ['Myosin VIIA']
@entity35 :: (10116,Species) :: ['rat']
