UNASSIGNED: Chromosome rearrangement is clinically and physiologically important because it can produce oncogenic fusion genes. Chromosome rearrangement requires DNA double-strand breaks (DSBs) at two genomic locations and misrejoining between the DSBs. Before DSB misrejoining, two DSB-containing chromatin regions move and pair with each other; however, the molecular mechanism underlying this process is largely unknown. We performed a spatiotemporal analysis of ionizing radiation-induced foci of @entity15664 ( @entity15664 ), a marker for DSB-containing chromatin. We found that some @entity15664 foci were paired, indicating that the two damaged chromatin regions neighboured one another. We searched for factors regulating the foci pairing and found that the number of paired foci increased when @entity10321 , @entity10322 , or ATM was absent. In contrast, @entity15664 depletion reduced the number of paired foci and dicentric chromosomes-an interchromosomal rearrangement. Foci were paired more frequently in heterochromatin than in euchromatin in control cells. Additionally, the reduced foci pairing in @entity15664 -depleted cells was rescued by concomitant depletion of a heterochromatin building factor such as Kr  ppel-associated box-associated protein 1 or @entity34255 . These findings indicate that pairing between DSB-containing chromatin regions was suppressed by @entity10321 , @entity10322 , and ATM, and this pairing was promoted by @entity15664 through chromatin relaxation.

Regulation of pairing between broken DNA-containing chromatin regions by @entity10321 , XXXX , ATM, and 53BP1.

@entity34255 :: ('1107', 'Gene') :: ['chromodomain helicase DNA-binding protein 3'] @entity10321 :: ('7520', 'Gene') :: ['Ku80'] @entity15664 :: ('7158', 'Gene') :: ['p53-binding protein 1', '53BP1'] @entity10322 :: ('5591', 'Gene') :: ['DNA-PKcs']
@entity10322 :: (5591,Gene) :: ['DNA-PKcs']
