In @entity375 UV radiation and a variety of chemical DNA-damaging agents induce the transcription of specific genes, including several involved in DNA repair. One of the best characterized of these genes is @entity69850 , which encodes the apoenzyme for DNA photolyase. Basal-level and damage-induced expression of @entity69850 require an upstream activation sequence, UAS( @entity69850 ), which has homology with DRC elements found upstream of at least 19 other DNA repair and DNA metabolism genes in @entity375 . Here we report the identification of the @entity39314 gene of @entity375 as a regulator of UAS( @entity69850 ) activity. Multiple copies of @entity39314 stimulate expression from UAS( @entity69850 ) and the intact @entity69850 gene. Surprisingly, the effect of deletion of @entity39314 is growth phase dependent. In wild-type cells @entity69850 is induced in late exponential phase, concomitant with the initiation of glycogen accumulation that precedes the diauxic shift. Deletion of @entity39314 abolishes this induction, decreases the steady-state concentration of photolyase molecules and @entity69850 mRNA, and increases the UV sensitivity of a @entity29814 mutant. Despite the fact that UAS( @entity69850 ) does not contain the URS1 sequence, which has been previously implicated in @entity39314 -mediated transcriptional regulation, we find that @entity39314 binds to UAS( @entity69850 ) with an affinity and a specificity similar to those seen for a URS1 site. Similar binding is also seen for DRC elements from @entity29814 , @entity33175 , and @entity16387 , suggesting that @entity39314 contributes to the regulated expression of a subset of damage-responsive genes in @entity375 .

Role of XXXX in transcriptional regulation of a DNA repair gene in @entity375 .

@entity69850 :: ('854568(Tax:4932)', 'Gene') :: ['PHR1'] @entity29814 :: ('853174(Tax:4932)', 'Gene') :: ['RAD2', 'rad2'] @entity16387 :: ('855950(Tax:4932)', 'Gene') :: ['RAD53'] @entity33175 :: ('853512(Tax:4932)', 'Gene') :: ['RAD7'] @entity375 :: ('4932', 'Species') :: ['yeast', 'Saccharomyces cerevisiae', 'S. cerevisiae'] @entity39314 :: ('851788(Tax:4932)', 'Gene') :: ['UME6', 'Ume6p']
@entity39314 :: (851788(Tax:4932),Gene) :: ['UME6']
