Kujin contains antiallergic compounds that inhibit upregulation of @entity112 H1 receptor ( @entity35089 ) and @entity12718 gene expression. However, the underlying mechanism remains unknown. We sought to identify a Kujin-derived antiallergic compound and investigate its mechanism of action. The @entity68942 mRNA levels were determined by real-time quantitative RT-PCR. To investigate the effects of maackiain in  vivo, @entity26678 ( @entity10345 )-sensitized @entity35 were used as a nasal hypersensitivity animal model. We identified (-)-maackiain as the responsible component. Synthetic maackiain showed stereoselectivity for the suppression of @entity12718 gene expression but not for @entity35089 gene expression, suggesting distinct target proteins for transcriptional signaling. @entity68943 inhibited of PKC   translocation to the Golgi and phosphorylation of Tyr(311) on PKC  , which led to the suppression of @entity35089 gene transcription. However, @entity68943 did not show any antioxidant activity or inhibition of PKC   enzymatic activity per se. Pretreatment with @entity68943 alleviated nasal symptoms and suppressed @entity10345 -induced upregulations of @entity35089 and @entity12718 gene expressions in @entity10345 -sensitized @entity35 . These data suggest that @entity68943 is a novel antiallergic compound that alleviates nasal symptoms in @entity10345 -sensitized @entity566 model @entity35 through the inhibition of @entity68942 gene expression. The molecular mechanism underlying its suppressive effect for @entity35089 gene expression is mediated by the inhibition of PKC   activation.

Maackiain is a novel antiallergic compound that suppresses transcriptional upregulation of the XXXX H1 receptor and @entity12718 genes.

@entity12718 :: ('287287(Tax:10116)', 'Gene') :: ['interleukin (IL)-4', 'IL-4'] @entity35089 :: ('24448(Tax:10116)', 'Gene') :: ['H1R'] @entity566 :: ('MESH:D006967', 'Disease') :: ['allergy'] @entity10345 :: ('CHEBI:53557', 'Chemical') :: ['TDI'] @entity26678 :: ('MESH:D014051', 'Chemical') :: ['toluene-2,4-diisocyanate'] @entity68942 :: ('24448;287287', 'Gene') :: ['H1R and IL-4'] @entity112 :: ('MESH:D006632', 'Chemical') :: ['histamine'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity68943 :: ('MESH:C001449', 'Chemical') :: ['(-)-maackiain', '(-)-Maackiain', 'maackiain']
@entity112 :: (MESH:D006632,Chemical) :: ['histamine']
