BACKGROUND: In @entity5 , it is mandatory to diagnose peritoneal @entity3 prior to selecting therapy. Therefore, exploratory laparoscopy has gained wider clinical acceptance. In laparoscopy, the peritoneal @entity3 is pathologically confirmed by  excisional  biopsy; however, there remain technical difficulties in performing precise diagnosis and adequate biopsy on small peritoneal lesions without damaging organs. We have focused on "optical biopsy" using probe-based confocal laser endomicroscopy (pCLE). The aims of this study were (1) to optimize current @entity30130 system for real-time observation of peritoneal metastases and (2) to assess its potential usefulness as diagnostic modality in preclinical settings. METHODS: To optimize condition and evaluate feasibility, we prepared peritoneal @entity3 @entity19 model with @entity356 cell line (MKN-45). On Day 10 after seeding, the @entity19 were laparotomized and performed pCLE observations with CellvizioLAB (LSU-F 400/488  nm, Mauna Kea Technologies, Paris, France). We evaluated two different @entity30130 probes, three different dyes, and optimal interval time. The detected sites were excised and pathologically evaluated on its morphology. Next, the feasibility and safety were validated in porcine model for clinical usage. After injection of @entity941 , pCLE was applied for the observation of intra-abdominal organs. RESULT: A miniature probe-type pCLE system with 60   m focal depth (UltraMini O) and 1  % @entity941 dye was chosen for good visualization in @entity19 model. The irregular microarchitecture images suspected to malignancy were obtained from the metastases. In the porcine model, observation of abdominal organs was feasible without any organ injury in the laparoscopic procedures. The dosage of 1  % @entity941 (3  ml/body) was appropriate in observing intra-abdominal organs, and each intra-abdominal organ was clearly observed with the same imaging quality we obtained in @entity19 model. CONCLUSION: The pCLE was feasible and safe and potentially useful for the diagnosis of the peritoneal @entity3 in in vivo animal models.

A novel approach of optical biopsy using probe-based confocal laser endomicroscopy for peritoneal XXXX .

@entity30130 :: ('MESH:C565836', 'Disease') :: ['CLE'] @entity5 :: ('MESH:D009369', 'Disease') :: ['digestive cancers'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity941 :: ('MESH:D019793', 'Chemical') :: ['fluorescein'] @entity356 :: ('MESH:D013274', 'Disease') :: ['gastric cancer']
@entity3 :: (MESH:D009362,Disease) :: ['metastasis']
