Na,K-ATPase generates the driving force for @entity1372 reabsorption in the kidney. Na,K-ATPase functional properties are regulated by small proteins belonging to the FXYD family. In kidney @entity68572 is the most abundant: it is an inhibitory subunit expressed in almost every nephron segment. Its absence should increase @entity1372 pump activity and promote Na(+) retention, however, no obvious renal phenotype was detected in @entity19 with global deletion of @entity68572 (Arystarkhova et al. 2013). Here, increased total cortical Na,K-ATPase activity was documented in the @entity68572 (-/-) @entity19 , without increased a1b1 subunit expression. We tested the hypothesis that adaptations occur in distal convoluted tubule (DCT), a major site of @entity1372 adjustments. Na,K-ATPase immunoreactivity in DCT was unchanged, and there was no DCT @entity509 . There was a marked activation of @entity2740 -sensitive @entity2827 cotransporter (NCC; @entity68573 ) in DCT, predicted to increase Na(+) reabsorption in this segment. Specifically, NCC total increased 30% and NCC phosphorylated at T53 and S71, associated with activation, increased 4-6 fold. The phosphorylation of the closely related thick ascending limb (TAL) apical @entity50236 ( @entity50236 ) increased at least twofold. Abundance of the total and cleaved (activated) forms of ENaC a-subunit was not different between genotypes. Nonetheless, no elevation of blood pressure was evident despite the fact that NCC and @entity50236 are in states permissive for Na(+) retention. Activation of NCC and @entity50236 may reflect an intracellular linkage to elevated Na,K-ATPase activity or a compensatory response to Na(+) loss proximal to the TAL and DCT.

Paradoxical activation of the XXXX cotransporter (NCC) without hypertension in kidney deficient in a regulatory subunit of Na,K-ATPase, @entity68572 .

@entity68573 :: ('20497(Tax:10090)', 'Gene') :: ['Slc12a3'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity68572 :: ('11936(Tax:10090)', 'Gene') :: ['FXYD2', 'Fxyd2'] @entity1372 :: ('MESH:D012964', 'Chemical') :: ['sodium'] @entity50236 :: ('20495(Tax:10090)', 'Gene') :: ['NKCC2', 'Slc12a1'] @entity509 :: ('MESH:C538429', 'Disease') :: ['hypoplasia'] @entity2740 :: ('CHEBI:50264', 'Chemical') :: ['thiazide'] @entity2827 :: ('MESH:D012965', 'Chemical') :: ['sodium chloride']
@entity2827 :: (MESH:D012965,Chemical) :: ['sodium chloride']
