INTRODUCTION: @entity4800 -based materials are recognized as bioactive materials through their capacity to induce hard tissue formation both in the dental pulp and bone. Sealing the apex implies that the root canal filling materials interact with the periapical tissues. This work was designed to study the interactions of newly developed @entity4800 cement (BioRoot RCS; Septodont, Saint Maur Des Fosses, France) with apical tissue compared with a standard @entity1947 - @entity4744 sealer (Pulp Canal Sealer [ @entity8847 ]; SybronEndo, Orange, CA). METHODS: Cell viability was assessed by direct contact between @entity1 periodontal ligament (PDL) cells and BioRoot RCS or @entity8847 . In addition, an in  vitro tooth model was used to study the interactions between these materials and PDL cells. For this purpose, @entity1 extracted incisors were sectioned at the enamel-cementum junction; root canals were prepared, sterilized, and filled with lateral condensation with both materials. The root apices were dipped in the culture medium for 24  hours. These conditioned media were used to investigate their effects on @entity1 PDL cells. Cell proliferation was investigated with the @entity2454 ( @entity98 ) assay, and the secretion of angiogenic and @entity421 was quantified using an enzyme-linked immunosorbent assay. RESULTS: BioRoot RCS has less toxic effects on PDL cells than @entity8847 and induced a higher secretion of angiogenic and @entity421 than @entity8847 . CONCLUSIONS: Taken together, these preclinical results suggest that the @entity4799 cement (BioRoot RCS) has a higher bioactivity than the @entity1947 - @entity4744 sealer ( @entity8847 ) on @entity1 PDL cells.

Bioactivity of a @entity4799 -based Endodontic Cement (BioRoot RCS): Interactions with XXXX Periodontal Ligament Cells In  Vitro.

@entity4744 :: ('MESH:D005054', 'Chemical') :: ['eugenol'] @entity1 :: ('9606', 'Species') :: ['human'] @entity1947 :: ('MESH:D015034', 'Chemical') :: ['zinc oxide'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity2454 :: ('MESH:C022616', 'Chemical') :: ['3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide'] @entity4800 :: ('MESH:C506393', 'Chemical') :: ['tricalcium silicate', 'Tricalcium silicate'] @entity421 :: ('MESH:D012516', 'Disease') :: ['osteogenic growth factors'] @entity8847 :: ('OMIM:176430', 'Disease') :: ['PCS'] @entity4799 :: ('MESH:C031293', 'Chemical') :: ['calcium silicate']
@entity1 :: (9606,Species) :: ['Human']
