Placental tissue injury is concomitant with @entity5 development. We investigated @entity5 -driven placental damage by tracing certain steps of the protein synthesis and degradation pathways under @entity368 -rich diet supplementation in MAC16-tumour-bearing @entity19 . Cell signaling and ubiquitin proteasome pathways were assessed in the placental tissues of pregnant @entity19 , which were distributed into three groups on a control diet (C- pregnant control, MAC- @entity5 -bearing pregnant, A- pregnant injected with MAC-ascitic fluid) and three other groups on a @entity368 -rich diet (L- pregnant, LMAC- @entity5 -bearing pregnant, LA- pregnant injected with MAC-ascitic fluid). MAC @entity5 growth down-regulated the cell signaling pathways of the placental tissue and decreased the levels of @entity3241 , @entity1994 , @entity13436 /MAPK, @entity3470 , @entity17740 , @entity10433 , and @entity9094 phosphorylated proteins, as assessed by the multiplex Millipore-Luminex assay. @entity368 supplementation maintained the levels of these proteins within the established cell signaling pathways. In the @entity5 -bearing group (MAC) only, the placenta tissue showed increased @entity34844 mRNA expression, as assessed by qRT-PCR, decreased 19S and 20S protein expression, as assessed by Western Blotting and decreased placental @entity669 levels likely reflecting up-regulation of the ubiquitin-proteasome pathway. Similar effects were found in the A group, confirming that the effects of the @entity5 were mimicked by MAC-ascitic fluid injection. Although @entity5 progression occurred, the degradation pathway-related protein levels were modulated under @entity368 -supplementation conditions. In conclusion, @entity5 evolution reduced the protein expression of the cell signaling pathway associated with elevated protein degradation, thereby jeopardizing the placental activity. Under the @entity368 -rich diet, the impact of @entity5 on placental function could be minimized by improving the cell signaling activity and reducing the proteolytic process.

A @entity368 -Rich Diet Modulates the Tumor-Induced Down-Regulation of the MAPK/ @entity13436 and PI3K/ @entity1994 / @entity3470 Signaling Pathways and Maintains the Expression of the Ubiquitin-Proteasome Pathway in the Placental Tissue of NMRI XXXX .

@entity368 :: ('CHEBI:25017', 'Chemical') :: ['leucine', 'Leucine'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity10433 :: ('20848(Tax:10090)', 'Gene') :: ['STAT3'] @entity17740 :: ('72508(Tax:10090)', 'Gene') :: ['p70S6K'] @entity9094 :: ('20852(Tax:10090)', 'Gene') :: ['STAT6'] @entity3470 :: ('21977(Tax:10090)', 'Gene') :: ['mTOR'] @entity34844 :: ('18552(Tax:10090)', 'Gene') :: ['PC5'] @entity13436 :: ('26413(Tax:10090)', 'Gene') :: ['Erk'] @entity1994 :: ('11651(Tax:10090)', 'Gene') :: ['Akt/PKB'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine'] @entity3241 :: ('16367(Tax:10090)', 'Gene') :: ['IRS-1']
@entity19 :: (10090,Species) :: ['Mice']
