BACKGROUND: The multidrug resistance pump, @entity7895 ( @entity7895 ), the @entity1262 gene expression product, is expressed in peripheral blood mononuclear cells (PBMC). @entity205 ( @entity870 ) and @entity1140 are inhibitors and substrates of the pump, and can induce its expression. The aim of the present study was to assess @entity1262 gene expression in PBMC after liver transplantation. METHODS: @entity7895 expression was examined in PBMC from 23 adult @entity1 by flow cytometry analysis using UIC2, a monoclonal anti- @entity7895 antibody, on days 7, 14, 21, 28, 90, and 180, and whenever a rejection episode was suspected. @entity1262 mRNA was estimated by reverse transcription-polymerase chain reaction. RESULTS: @entity7895 expression, assessed as the ratio of the mean fluorescence, increased from 1.59 before transplantation to 2.05 after transplantation, and did not vary significantly thereafter. There was no significant difference in @entity7895 expression between @entity870 and @entity1140 -based treatments. @entity1262 mRNA levels did not vary significantly under @entity870 or @entity1140 therapy until day 28. @entity7895 expression was marginally higher in @entity1 before acute rejection (P=0.054), and was lower in @entity1 with severe @entity281 (P=0.003) than in those with uneventful evolution. Alcoholic @entity1 exhibited higher levels of @entity7895 expression than other @entity1 . CONCLUSION: @entity7895 expression increases after liver transplantation. Its variations do not allow the ability to predict acute rejection, but could help in making the diagnosis of overimmunosuppression.

XXXX gene expression in peripheral blood mononuclear cells after liver transplantation.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity1262 :: ('5243', 'Gene') :: ['MDR1'] @entity870 :: ('CHEBI:55379', 'Chemical') :: ['CsA'] @entity205 :: ('MESH:D016572', 'Chemical') :: ['Cyclosporine'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity7895 :: ('283871', 'Gene') :: ['P-gp', 'Pgp', 'P-glycoprotein 170'] @entity1140 :: ('MESH:D016559', 'Chemical') :: ['tacrolimus']
@entity1262 :: (5243,Gene) :: ['MDR1']
