MicroRNAs (miRNAs) comprise a novel class of endogenous, small, noncoding RNAs that negatively regulate approximately 30% of genes in a cell via degradation or translational inhibition of their target mRNAs. However, the effects of reactive @entity26 species (ROS) on miRNA expression and the roles of miRNAs in ROS-mediated gene regulation and biological functions of vascular cells are unclear. Using microarray analysis, we demonstrated that miRNAs are aberrantly expressed in vascular smooth muscle cells (VSMCs) after treatment with @entity273 ( @entity274 ). @entity274 -mediated up-regulation of @entity10017 ( @entity10017 ) was further confirmed by quantitative real-time PCR. To determine the potential roles of miRNAs in @entity274 -mediated gene regulation and cellular effects, @entity10017 expression was down-regulated by @entity10017 inhibitor and up-regulated by pre- @entity10017 . @entity274 -induced VSMC apoptosis and @entity204 were increased by @entity10017 inhibitor and decreased by pre- @entity10017 . Programmed cell @entity204 4( @entity18239 ) was a direct target of @entity10017 that was involved in @entity10017 -mediated effects on VSMCs. Pre- @entity10017 -mediated protective effect on VSMC apoptosis and @entity204 was blocked via adenovirus-mediated overexpression of @entity18239 without the @entity10017 binding site. Moreover, @entity11346 was a downstream signaling molecule of @entity18239 in @entity10017 -modulated VSMCs. The results suggest that miRNAs in VSMCs are sensitive to @entity274 stimulation. @entity10017 participates in @entity274 -mediated gene regulation and cellular injury response through @entity18239 and the @entity11346 pathway. miRNAs might play a role in @entity455 related to ROS.

Involvement of MicroRNAs in XXXX -mediated gene regulation and cellular injury response in vascular smooth muscle cells.

@entity455 :: ('MESH:D014652', 'Disease') :: ['vascular diseases'] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H(2)O(2)'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['hydrogen peroxide'] @entity10017 :: ('406991', 'Gene') :: ['miRN-21', 'miR-21', 'microRNA-21'] @entity11346 :: ('3726', 'Gene') :: ['activator protein 1'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death'] @entity18239 :: ('27250', 'Gene') :: ['PDCD4']
@entity273 :: (MESH:D006861,Chemical) :: ['hydrogen peroxide']
