In the @entity1 brain, the transformation of radial glial cells (RGC) into astrocytes has been studied only rarely. In this work, we were interested in studying the morphologic aspects underlying this transformation during the fetal/perinatal period, particularly emphasizing the region-specific glial fiber anatomy in the medial cortex. We have used @entity18788 dyes ( @entity18516 / @entity19552 ) to identify the RGC transitional forms and glial fiber morphology. Immunocytochemical markers such as vimentin and @entity538 ( @entity538 ) were also employed to label the radial cells of glial lineage and to reveal the early pattern of astrocyte distribution. Neuronal markers such as @entity10946 ( @entity10946 ) and @entity7333 ( @entity14383 ) were employed to discern whether or not these radial cells could, in fact, be neurons or neuronal precursors. The main findings concern the beginning of RGC transformation showing loss of the ventricular fixation in most cases, followed by transitional figures and the appearance of mature astrocytes. In addition, diverse fiber morphology related to depth within the cortical mantle was clearly demonstrated. We concluded that during the fetal/perinatal period the cerebral cortex is undergoing the final stages of radial neuronal migration, followed by involution of RGC ventricular processes and transformation into astrocytes. None of the transitional or other radial glia were positive for neuronal markers. Furthermore, the differential morphology of RGC fibers according to depth suggests that factors may act locally in the subplate and could have a role in the process of cortical RGC transformation and astrocyte localization. The early pattern of astrocyte distribution is bilaminar, sparing the cortical plate. Few astrocytes ( @entity538 +) in the upper band could be found with radial processes at anytime. This suggests that astrocytes in the marginal zone could be derived from different precursors than those that differentiate from RGCs during this period.

Cortical radial glial cells in XXXX fetuses: depth-correlated transformation into astrocytes.

@entity1 :: ('9606', 'Species') :: ['human'] @entity7333 :: ('51115', 'Gene') :: ['microtubule-associated protein'] @entity18788 :: ('CHEBI:52218', 'Chemical') :: ['carbocyanine'] @entity14383 :: ('4133', 'Gene') :: ['MAP-2'] @entity19552 :: ('MESH:C076868', 'Chemical') :: ['DiA'] @entity538 :: ('2670', 'Gene') :: ['GFAP', 'glial fibrillary acidic protein'] @entity10946 :: ('146713', 'Gene') :: ['NeuN', 'neuronal-specific nuclear protein'] @entity18516 :: ('CHEBI:52029', 'Chemical') :: ['DiI']
@entity1 :: (9606,Species) :: ['human']
