UNASSIGNED: Excessive or unfolded proteins accumulation in endoplasmic reticulum (ER) will cause ER stress, which has evolved to involve in various @entity209 . In particular, ER stress plays an important role in the pathogenesis of @entity6 . Both ER stress and course of @entity6 accompany oxidative stress and production of reactive @entity26 species (ROS), among which @entity224 anion ( @entity3612 ) is the first produced ROS and has been recognized as cell signaling mediator involved in the physiological and pathological process of @entity6 . Hence, the development of effective monitoring methods of @entity3612 in live cells and in vivo is of great importance for ascertaining the onset and progress of @entity66 . Herein, a new endoplasmic reticulum-targeted two-photon fluorescent probe termed ER-BZT is designed and synthesized for imaging of @entity3612 . The probe ER-BZT shows high sensitivity, selectivity, stability, and low @entity137 . Based on these superior properties, the rise of @entity3612 levels in endoplasmic reticulum induced with different stimuli is visualized by one- and two-photon fluorescence imaging. Most importantly, by utilizing ER-BZT, the two-photon fluorescence imaging results demonstrate that the endogenous @entity3612 concentration in abdominal or hepatic tissue of @entity6 @entity19 is higher than that in normal @entity19 . Meanwhile, after treated with @entity2449 , a broad-spectrum antidiabetic drug, the @entity6 @entity19 exhibit @entity308 (   -) level. The proposed two-photon probe, ER-BZT might serve as perfect tool to image the @entity3612 fluctuations and study the relevance between @entity3612 and various diseases in live cells and in vivo.

A new endoplasmic reticulum-targeted two-photon fluorescent probe for imaging of @entity224 anion in @entity6 XXXX .

@entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity308 :: ('MESH:D003866', 'Disease') :: ['depressed O2'] @entity66 :: ('MESH:D004194', 'Disease') :: ['related diseases'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetic', 'diabetes'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity3612 :: ('D013481', 'Chemical') :: ['O2(   -)'] @entity209 :: ('MESH:D008659', 'Disease') :: ['metabolic diseases'] @entity224 :: ('MESH:D013481', 'Chemical') :: ['superoxide'] @entity2449 :: ('MESH:D008687', 'Chemical') :: ['metformin']
@entity19 :: (10090,Species) :: ['mice']
