BACKGROUND: The local skin @entity68168 procedure is very useful for reconstruction. However, @entity68168 @entity39 caused by @entity1457 can occur at its distal portion. @entity519 -inducible factors (HIFs) in endothelial cells (ECs) help to maintain ECs and promote vascularization, and @entity22168 is abundantly expressed in ECs. However, the mechanisms of action of @entity22168 in ECs are not yet fully understood. The aim of this study was to evaluate the in vivo effects of overexpression of @entity22168 in ECs on skin @entity68168 survival. METHODS: A random pattern skin @entity68168 (1.0   3.0 cm) was elevated on the dorsum of @entity19 (Tg @entity19 ) with EC-specific @entity22168 conditional overexpression and wild-type littermate control @entity19 (n = 6). @entity68168 survival was evaluated on postoperative day 7. Tissue samples from the skin flaps were harvested and analyzed using Western blotting, quantitative reverse transcriptase-polymerase chain reaction, and immunohistochemistry. RESULTS: The @entity22168 mRNA and protein levels were significantly increased in the Tg @entity19 when compared with control @entity19 . Tg @entity19 had significantly increased skin @entity68168 survival areas (72.0%    2.7%) when compared with wild-type @entity19 (45.7%    1.1%). Moreover, histological examination revealed an increase in the subcutaneous blood vessel counts in the Tg @entity19 . CONCLUSIONS: Specific overexpression of @entity22168 in ECs promoted vascularization and enhanced skin @entity68168 survival in vivo in a @entity19 model.

Overexpressed @entity22168 in Endothelial Cells Promotes Vascularization and Improves Random Pattern Skin XXXX Survival.

@entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'transgenic mice'] @entity1457 :: ('MESH:D012769', 'Disease') :: ['circulatory failure'] @entity519 :: ('MESH:D000860', 'Disease') :: ['Hypoxia'] @entity22168 :: ('13819(Tax:10090)', 'Gene') :: ['HIF-2a'] @entity68168 :: ('11690(Tax:10090)', 'Gene') :: ['Flap', 'flap']
@entity68168 :: (11690(Tax:10090),Gene) :: ['Flap']
