BACKGROUND: @entity9138 availability is involved in glycolytic flux and fermentation efficiency. A deficiency of this vitamin may be responsible for sluggish fermentations in wine making. Therefore, both @entity9138 uptake and de novo synthesis could have key roles in fermentation processes. @entity9138 biosynthesis is regulated in response to @entity9138 availability and is coordinated by the @entity9138 sensor @entity53778 , which activates @entity68519 and @entity68520 . We used a genetic approach to identify quantitative trait loci (QTLs) in wine @entity375 and we discovered that a set of @entity9138 genes displayed expression-QTL on a common locus, which contains the @entity9138 regulator @entity53778 . RESULTS: We deciphered here the source of these regulatory variations of the THI and PDC genes. We showed that alteration of @entity53778 results in reduced expression of the genes involved in @entity9138 biosynthesis ( @entity68521 /12/13 and @entity68522 ) and increased expression of the @entity2388 decarboxylase gene @entity37079 . Functional analysis of the allelic effect of @entity53778 confirmed the control of the THI and @entity37079 genes. We observed, however, only a small effect of the @entity53778 on fermentation kinetics. We demonstrated that the expression levels of several THI genes are correlated with fermentation rate, suggesting that decarboxylation activity could drive gene expression through a modulation of @entity9138 content. Our data also reveals a new role of @entity53778 in the regulation of the main @entity2388 decarboxylase gene, @entity37079 . CONCLUSIONS: This highlights a switch from @entity37079 to @entity68523 gene expression during @entity9138 deficiency, which may improve the @entity9138 affinity or conservation during the enzymatic reaction. In addition, we observed that the lab allele of @entity53778 and of the @entity15854 transporter @entity53775 have diverged from the original alleles, consistent with an adaptation of lab strains to rich media containing an excess of @entity9138 .

Deciphering regulatory variation of THI genes in alcoholic fermentation indicate an impact of @entity53778 on XXXX expression.

@entity53775 :: ('850938(Tax:4932)', 'Gene') :: ['THI7'] @entity9138 :: ('MESH:D013831', 'Chemical') :: ['Thiamine', 'thiamine'] @entity68521 :: ('853621(Tax:4932)', 'Gene') :: ['THI11'] @entity68522 :: ('852048(Tax:4932)', 'Gene') :: ['THI74'] @entity68519 :: ('851654(Tax:4932)', 'Gene') :: ['Pdc2p'] @entity15854 :: ('CHEBI:33283', 'Chemical') :: ['thiamin'] @entity68523 :: ('850825(Tax:4932)', 'Gene') :: ['PDC5'] @entity53778 :: ('851479(Tax:4932)', 'Gene') :: ['THI3', 'Thi3p'] @entity68520 :: ('852542(Tax:4932)', 'Gene') :: ['Thi2p'] @entity375 :: ('4932', 'Species') :: ['yeast'] @entity37079 :: ('850733(Tax:4932)', 'Gene') :: ['PDC1'] @entity2388 :: ('MESH:D019289', 'Chemical') :: ['pyruvate']
@entity37079 :: (850733(Tax:4932),Gene) :: ['PDC1']
