@entity876 undergoes phosphorylation on several residues in response to cellular stresses that include UV and ionizing radiation, however the influence of spindle damage on this parameter is relatively unclear. Consequently, the effect of @entity10612 on @entity563 392 phosphorylation was examined in two epithelial cell lines. We show that this process is dependent upon the stepwise activation of @entity1435 ( @entity1435 ) and protein kinase casein kinase 2 (CK2). Furthermore, this activation correlated with the biochemical regulation of the maturation-promoting factor (MPF, @entity10610 /cyclin B), as both DRB and antisense depletion of CK2, as well as @entity1441 were associated with an inhibition of its activation in response to @entity10612 . Strikingly, when the cell cycle characteristics of @entity10612 treated cells were examined, we observed that depletion or inhibition of the catalytic subunit of CK2, in the presence of microtubule inhibitors, resulted in a compromise of the @entity1167 (spindle checkpoint). Furthermore, CK2-depleted, @entity10612 treated cells demonstrated a dramatic reduction in the apoptotic cell fraction, confirming that these cells had been endowed with oncogenic properties. These changes were observed in both HeLa cells and HCT116 cells. We also show that this effect is dependent on the presence of functional wild-type @entity876 , as this phenomenon is not apparent in HCT116 @entity876 (-/-) cells. Collectively, our results indicate two novel roles for CK2 in the spindle checkpoint arrest, in concert with @entity876 . Firstly, to maintain increased cyclinB/ @entity10610 kinase activity, as a component of @entity1167 , and secondly, a role in @entity876 -mediated apoptosis. These findings may have implications for an improved understanding of abnormalities of the spindle checkpoint in @entity1 @entity5 , which is a prerequisite for defining future therapies.

Protein kinase CK2 is involved in XXXX and apoptosis following spindle damage in epithelial cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity10610 :: ('983', 'Gene') :: ['cdc2'] @entity563 :: ('CHEBI:17822', 'Chemical') :: ['serine'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancers'] @entity10612 :: ('MESH:D015739', 'Chemical') :: ['nocodazole'] @entity1441 :: ('MESH:C093642', 'Chemical') :: ['SB203580'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity1435 :: ('1432', 'Gene') :: ['p38 mitogen-activated protein kinase', 'p38 MAPK'] @entity1167 :: ('MESH:D006323', 'Disease') :: ['G2 arrest']
@entity1167 :: (MESH:D006323,Disease) :: ['G2 arrest']
