We previously described a kinesin-dependent movement of particles in the flagella of @entity2060 called intraflagellar transport (IFT) (Kozminski, K.G., K.A. Johnson, P. Forscher, and J.L. Rosenbaum. 1993. Proc. Natl. Acad. Sci. USA. 90:5519-5523). When IFT is inhibited by inactivation of a kinesin, @entity34063 , in the temperature-sensitive mutant, @entity34063 , existing @entity4536 cannot be assembled. We report here that: (a) the IFT-associated @entity34063 protein is a subunit of a heterotrimeric kinesin; (b) IFT particles are composed of 15 polypeptides comprising two large complexes; (c) the @entity34063 kinesin-II and IFT particle polypeptides, in addition to being found in flagella, are highly concentrated around the flagellar basal bodies; and, (d) mutations affecting homologs of two of the IFT particle polypeptides in @entity2199 result in defects in the sensory cilia located on the dendritic processes of sensory neurons. In the accompanying report by Pazour, G.J., C.G. Wilkerson, and G.B. Witman (1998. J. Cell Biol. 141:979-992), a @entity2060 mutant ( @entity69423 ) is described in which only the retrograde transport of IFT particles is disrupted, resulting in @entity20740 filled with an excess of IFT particles. This microtubule- dependent transport process, IFT, defined by mutants in both the anterograde ( @entity34063 ) and retrograde ( @entity69423 ) transport of isolable particles, is probably essential for the maintenance and assembly of all @entity1319 and nonmotile sensory cilia.

XXXX kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in @entity2199 sensory neurons.

@entity2199 :: ('6239', 'Species') :: ['Caenorhabditis elegans'] @entity1319 :: ('MESH:D015835', 'Disease') :: ['eukaryotic motile flagella'] @entity69423 :: ('5728480(Tax:3055)', 'Gene') :: ['fla14'] @entity4536 :: ('MESH:D016773', 'Disease') :: ['flagella resorb and new flagella'] @entity34063 :: ('5727085(Tax:3055)', 'Gene') :: ['fla10', 'FLA10'] @entity2060 :: ('3055', 'Species') :: ['Chlamydomonas', 'Chlamydomonas reinhardtii'] @entity20740 :: ('MESH:C564991', 'Disease') :: ['assembly-defective flagella']
@entity2060 :: (3055,Species) :: ['Chlamydomonas']
