The objectives of this study were to evaluate the morphology and biomechanical function of Achilles tendons regenerated using knitted @entity10124 ( @entity7050 ) loaded with bone marrow stromal cells (bMSCs). The animal model used was that of an adult female New Zealand White @entity95 with a 10-mm gap defect of the Achilles tendon. In group I, 19 hind legs with the created defects were treated with allogeneic bMSCs seeded on knitted @entity7050 scaffold. In group II, the @entity924 in 19 hind legs were repaired using the knitted @entity7050 scaffold alone, and in group III, 6 hind legs were used as normal control. The tendon-implant constructs of groups I and II were evaluated postoperatively at 2, 4, 8, and 12 weeks using macroscopic, histological, and immunohistochemical techniques. In addition, specimens from group I (n = 7), group II (n = 7), and group III (n = 6) were harvested for biomechanical test 12 weeks after surgery. Postoperatively, at 2 and 4 weeks, the histology of group I specimens exhibited a higher rate of tissue formation and remodeling as compared with group II, whereas at 8 and 12 weeks postoperation, the histology of both group I and group II was similar to that of native tendon tissue. The wound sites of group I healed well and there was no apparent lymphocyte infiltration. Immunohistochemical analysis showed that the regenerated tendons were composed of collagen types I and type III fibers. The tensile stiffness and modulus of group I were 87 and 62.6% of normal tendon, respectively, whereas those of group II were about 56.4 and 52.9% of normal tendon, respectively. These results suggest that the knitted @entity7050 biodegradable scaffold loaded with allogeneic bone marrow stromal cells has the potential to regenerate and repair gap defect of Achilles tendon and to effectively restore structure and function.

Knitted XXXX scaffold loaded with bone marrow stromal cells in repair and regeneration of @entity95 Achilles tendon.

@entity7050 :: ('MESH:C043435', 'Chemical') :: ['PLGA'] @entity924 :: ('MESH:D052256', 'Disease') :: ['Achilles tendon defects'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity10124 :: ('CHEBI:53493', 'Chemical') :: ['poly-lactide-co-glycolide']
@entity10124 :: (CHEBI:53493,Chemical) :: ['poly-lactide-co-glycolide']
