DNA probe immobilization on plastic surfaces and device assembly are both critical to the fabrication of @entity8625 ( @entity8625 ) devices. Three oligonucleotide (oligo) probe immobilization procedures were investigated for attaching oligo probes on four different types of plastic surfaces ( @entity852 , @entity6027 , @entity4625 , and @entity7502 ). These procedures are the Surmodics procedure, the @entity14942 ( @entity14942 ) procedure, and the Reacti-Bind procedure. To determine the optimal plastic substrate and attachment chemistry for array fabrication, we investigated plastic hydrophobicity, intrinsic fluorescence, and oligo attachment efficiency. The Reacti-Bind procedure is least effective for attaching oligo probes in the microarray format. The @entity14942 procedure performs well enough to use in array fabrication, and the concentration of @entity14942 has a significant effect on oligo immobilization efficiency. We also found that use of @entity6115 -modified oligo probes resulted in better immobilization efficiency than use of unmodified oligos with the @entity14942 procedure. The oligo probe immobilization on plastic surfaces by the Surmodics procedure is the most effective with regard to probe spot quality and hybridization sensitivity. A DNA hybridization assay on such a device results in a limit of detection of 12pM. Utilizing a @entity515 IR laser machining and adhesive layer approach, we have developed an improved procedure for realizing a DNA microarray inside a microfluidic channel. This device fabrication procedure allows for more feasible spot placement in the channel and reduced sample adsorption by adhesive tapes used in the fabrication procedure. We also demonstrated improved hybridization kinetics and increased detection sensitivity in @entity8625 devices by implementing sample oscillation inside the channel. A limit of detection of 5pM has been achieved in @entity8625 devices with sample oscillation.

DNA probe attachment on plastic surfaces and XXXX devices with sample oscillation.

@entity4625 :: ('MESH:D019904', 'Chemical') :: ['poly(methylmethacrylate)'] @entity852 :: ('MESH:D011137', 'Chemical') :: ['polystyrene'] @entity6027 :: ('CHEBI:61433', 'Chemical') :: ['polycarbonate'] @entity7502 :: ('MESH:D011126', 'Chemical') :: ['polypropylene'] @entity6115 :: ('CHEBI:32952', 'Chemical') :: ['amine'] @entity515 :: ('CHEBI:29334', 'Chemical') :: ['CO(2)'] @entity8625 :: ('MESH:D015456', 'Disease') :: ['MHAC', 'microfluidic hybridization array channel'] @entity14942 :: ('MESH:C004322', 'Chemical') :: ['CTAB', 'cetyltrimethylammonium bromide']
@entity8625 :: (MESH:D015456,Disease) :: ['microfluidic hybridization array channel']
