Persistent @entity9 is associated with @entity148 . The phosphorylation of extracellular signal-regulated kinase (p-ERK) in the anterior cingulate cortex (ACC) plays an important role in @entity158 -induced @entity148 . Acupuncture is widely used for @entity158 and @entity148 . However, little is known about which acupuncture technique is optimal on @entity158 -induced @entity148 and the relationship between acupuncture effect and p-ERK. The @entity35 model was induced by L5 spinal nerve ligation (SNL). Male adult SD @entity35 were randomly divided into control, SNL, strong manual acupuncture (sMA), mild manual acupuncture (mMA), and electroacupuncture (EA) group. Bilateral "Huantiao" (GB 30) were stimulated by sMA, mMA, and EA, respectively. The @entity158 withdrawal thresholds (PWTs) and @entity146 were measured, and p-ERK protein expression and immunoreactivity cells in ACC were detected. PWTs increased significantly in both sMA and EA groups. Meanwhile, @entity148 -like behavior was improved significantly in the sMA and mMA groups. Furthermore, the overexpression of p-ERK induced by SNL was downregulated by strong and mild manual acupuncture. Therefore, strong manual acupuncture on bilateral "Huantiao" (GB 30) could be a proper therapy relieving both @entity158 and @entity158 -induced @entity148 . The effect of different acupuncture techniques on @entity158 -induced @entity148 may arise from the regulation of p-ERK in ACC.

Strong Manual Acupuncture Stimulation of "Huantiao" (GB 30) Reduces @entity158 and p-ERK in the Anterior Cingulate Cortex in a XXXX Model of @entity9 .

@entity146 :: ('MESH:D001523', 'Disease') :: ['anxiety behavior'] @entity148 :: ('MESH:D001008', 'Disease') :: ['anxiety'] @entity158 :: ('MESH:D010146', 'Disease') :: ['pain'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity9 :: ('MESH:D009437', 'Disease') :: ['neuropathic pain']
@entity35 :: (10116,Species) :: ['Rat']
