In search of novel serological protein biomarkers for @entity1 @entity14 ( @entity14 ), we analyzed @entity14 tissues using two-dimensional difference in-gel electrophoresis (2D-DIGE) on a narrow range IPG strip (pH 5.5-6.7). By comparing @entity5 tissues with matched normal tissues in a pairwise manner (n = 6), we identified 34 up-regulated and 17 down-regulated spots with intensity changes greater than 2-fold (Student's t-test, p < 0.05). Expression of both mRNA and protein levels of four proteins, adenosylhomocysteinase, @entity18962 , @entity2757 and @entity7966 , in @entity14 tissues was further evaluated by semiquantitative RT-PCR and Western blot analysis. The results revealed that all four proteins were elevated in the @entity5 tissues. We also confirmed, by immunohistochemistry, that adenosylhomocysteinase and @entity18962 were overexpressed in @entity5 cell cytoplasm and that @entity2757 and @entity7966 proteins were strongly expressed in @entity5 infiltrating immune cells. Western blot analysis with fractionated plasma samples showed that @entity2757 and @entity7966 were significantly increased in the plasma of @entity14 @entity1 (n = 77) and @entity2513 @entity1 (n = 11), compared to healthy controls (n = 21). The area under a receiver operating characteristic (ROC) curve was 0.91 for @entity2757 and 0.89 for @entity7966 , which was superior to the established @entity5 marker carcinoembryonic antigen with 0.78 for the area under the ROC curve. Some @entity1 with @entity32 such as @entity788 also showed elevated levels of the proteins. Importantly, in comparison to the control group, both proteins showed a remarkable change at the early stage of @entity5 . Therefore, we suggest @entity2757 and @entity7966 as candidates for serological biomarkers in combination with other serum markers that aid @entity14 diagnosis.

Identification of XXXX and @entity7966 as serological markers for @entity14 .

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammatory diseases'] @entity788 :: ('MESH:D010190', 'Disease') :: ['pancreatitis'] @entity7966 :: ('6280', 'Gene') :: ['S100A9'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity18962 :: ('4830', 'Gene') :: ['Nm23-H1'] @entity14 :: ('MESH:D015179', 'Disease') :: ['CRC', 'colorectal cancer'] @entity2757 :: ('6279', 'Gene') :: ['S100A8'] @entity2513 :: ('MESH:C563365', 'Disease') :: ['colorectal adenoma']
@entity2757 :: (6279,Gene) :: ['S100A8']
