This study aimed to investigate whether oxidative stress contributes to retinal cell death in a @entity19 model of @entity528 induced by @entity7044 ( @entity7045 ). We measured in vitro @entity7045 -induced radical production in retinal cell cultures of @entity19 661W photoreceptor-derived cells; RGC-5, a @entity19 ganglion cell line; and primary retinal cells. The addition of @entity7045 induced oxidative radical generation in 661W and primary retinal cells, but not in RGC-5 cells. @entity4465 , a free radical scavenger, at 1   M reduced @entity7045 -induced radical production in 661W and primary retinal cells. To induce in vivo @entity2000 in @entity19 , we administered 60 mg/kg @entity7045 by intraperitoneal injection. We intravenously administered 1 mg/kg @entity4465 immediately and at 6 h after the @entity7045 injection. @entity2000 was evaluated by measuring the thickness of the outer nuclear layer (ONL) by terminal deoxynucleotidyl transferase @entity1977 nick-end labeling (TUNEL) staining and by oxidative stress markers. @entity7045 caused photoreceptor cell loss at 7 days after administration. @entity4465 inhibited ONL thinning and reduced TUNEL-positive cells and the oxidative stress markers. These findings indicate that @entity7045 leads to selective photoreceptor degradation via oxidative stress in vitro and in vivo and may help to understand the pathogenic mechanism of @entity6391 .

Role of oxidative stress in retinal photoreceptor cell death in XXXX -treated @entity19 .

@entity7045 :: ('CHEBI:50102', 'Chemical') :: ['MNU'] @entity2000 :: ('MESH:D012162', 'Disease') :: ['retinal photoreceptor degeneration', 'Retinal photoreceptor degeneration'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'murine'] @entity4465 :: ('MESH:C005435', 'Chemical') :: ['edaravone', 'Edaravone'] @entity1977 :: ('CHEBI:17625', 'Chemical') :: ['dUTP'] @entity6391 :: ('MESH:D012174', 'Disease') :: ['retinitis pigmentosa'] @entity7044 :: ('MESH:D008770', 'Chemical') :: ['N-methyl-N-nitrosourea'] @entity528 :: ('MESH:D009410', 'Disease') :: ['photoreceptor degeneration']
@entity7044 :: (MESH:D008770,Chemical) :: ['N-methyl-N-nitrosourea']
