UNASSIGNED: Ribosomes account for a majority of the cell's RNA and much of its protein and represent a significant investment of cellular resources. The turnover and degradation of ribosomes has been proposed to play a role in homeostasis and during stress conditions. Mechanisms for the turnover of rRNA and ribosomal proteins have not been fully elucidated. We show here that the @entity69229 ribonuclease and autophagy participate in RNA turnover in @entity958 under normal growth conditions. An increase in autophagosome formation was seen in an @entity69229 -2 mutant, and this increase was dependent on the core autophagy genes ATG9 and @entity69230 . Autophagosomes and autophagic bodies in @entity69229 -2 mutants contain RNA and ribosomes, suggesting that autophagy is activated as an attempt to compensate for loss of rRNA degradation. Total RNA accumulates in @entity69229 -2, atg9-4, @entity69230 -1, @entity69229 -2 atg9-4, and @entity69229 -2 @entity69230 -1 mutants, suggesting a parallel role for autophagy and @entity69229 in RNA turnover. rRNA accumulates in the vacuole in @entity69229 -2 mutants. Vacuolar accumulation of rRNA was blocked by disrupting autophagy via an @entity69229 -2 @entity69230 -1 double mutant but not by an @entity69229 -2 atg9-4 double mutant, indicating that @entity69230 and ATG9 function differently in this process. Our results suggest that autophagy and @entity69229 are both involved in homeostatic degradation of rRNA in the vacuole.

Evidence for autophagy-dependent pathways of rRNA turnover in XXXX .

@entity958 :: ('3702', 'Species') :: ['Arabidopsis thaliana'] @entity69229 :: ('818563(Tax:3702)', 'Gene') :: ['RNS2', 'rns2'] @entity69230 :: ('831594(Tax:3702)', 'Gene') :: ['ATG5', 'atg5']
@entity958 :: (3702,Species) :: ['Arabidopsis']
