UNASSIGNED: Blood-brain barrier (BBB) dysfunction is considered to be an early event in the pathogenesis of a variety of @entity16 in old @entity1 , and this could occur in old @entity1 even when facing common stress. However, the mechanism remains to be defined. In this study, we tested the hypothesis that decreased @entity2493 levels may account for the BBB disruption in old @entity19 challenged with lipopolysaccharide (LPS), which mimicked the common stress of @entity295 . @entity19 (24-28  months of age) received @entity2493 (10  mg  kg(-1)   day(-1) , intraperitoneally, i.p.) or saline for one week before exposing to LPS (1  mg  kg(-1) , i.p.). @entity23045 dye (EB) and immunoglobulin G (IgG) leakage were used to assess BBB permeability. Immunostaining and Western blot were used to detect protein expression and distribution. Our results showed that LPS significantly increased BBB permeability in old @entity19 accompanied by the degradation of tight junction proteins @entity8843 and @entity14880 , suppressed @entity8582 -activated protein kinase (AMPK) activation, and elevated @entity18511 (phox) protein expression. Interestingly, administration of @entity2493 for one week significantly decreased LPS-induced BBB disruption, AMPK suppression, and @entity18511 (phox) upregualtion. Moreover, activation of AMPK with @entity2449 significantly inhibited LPS-induced @entity18511 (phox) upregualtion in endothelial cells. Taken together, our findings demonstrate that @entity2493 alleviates LPS-induced BBB disruption through activating AMPK and inhibiting @entity18511 (phox) upregulation in old @entity19 .

@entity2493 alleviates lipopolysaccharide-compromised integrity of blood-brain barrier through activating @entity8582 -activated protein kinase in old XXXX .

@entity1 :: ('9606', 'Species') :: ['patients', 'people'] @entity295 :: ('MESH:D018805', 'Disease') :: ['sepsis'] @entity14880 :: ('12741(Tax:10090)', 'Gene') :: ['claudin-5'] @entity8843 :: ('18260(Tax:10090)', 'Gene') :: ['occludin'] @entity19 :: ('10090', 'Species') :: ['mice', 'Mice'] @entity16 :: ('MESH:D009422', 'Disease') :: ['neurological diseases'] @entity23045 :: ('MESH:D005070', 'Chemical') :: ["Evan's blue"] @entity18511 :: ('18733(Tax:10090)', 'Gene') :: ['gp91'] @entity2493 :: ('MESH:D008550', 'Chemical') :: ['melatonin'] @entity8582 :: ('D000667', 'Chemical') :: ['AMP'] @entity2449 :: ('MESH:D008687', 'Chemical') :: ['metformin']
@entity19 :: (10090,Species) :: ['mice']
