UNASSIGNED: @entity981 ( @entity981 ) initiates and progresses in the bone marrow, and as such, the marrow microenvironment is a critical regulatory component in development of this @entity5 . However, @entity981 studies were conducted mainly on flat plastic substrates, which do not recapitulate the characteristics of marrow microenvironments. To study @entity981 in a model of in vivo relevance, we have engineered a 3-D microfluidic cell culture platform. Biologically relevant populations of primary @entity1 bone marrow stromal cells, osteoblasts and @entity1 leukemic cells representative of an @entity146 phenotype were encapsulated in 3-D collagen matrix as the minimal constituents and cultured in a microfluidic platform. The matrix stiffness and fluidic shear stress were controlled in a physiological range. The 3-D microfluidic as well as 3-D static models demonstrated coordinated cell-cell interactions between these cell types compared to the compaction of the 2-D static model. @entity5 cell viability in response to an antimetabolite chemotherapeutic agent, @entity2592 in @entity5 cells alone and tri-culture models for 2-D static, 3-D static and 3-D microfluidic models were compared. The present study showed decreased chemotherapeutic drug sensitivity of leukemic cells in 3-D tri-culture models from the 2-D models. The results indicate that the bone marrow microenvironment plays a protective role in @entity5 cell survival during drug treatment. The engineered 3-D microfluidic tri-culture model enables systematic investigation of effects of cell-cell and cell-matrix interactions on @entity5 progression and therapeutic intervention in a controllable manner, thus improving our limited comprehension of the role of microenvironmental signals in @entity5 biology.

Three-Dimensional Microfluidic Tri-Culture Model of the Bone Marrow Microenvironment for Study of XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity981 :: ('MESH:D054198', 'Disease') :: ['Acute lymphoblastic leukemia', 'ALL'] @entity146 :: ('MESH:D001523', 'Disease') :: ['aggressive'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'Tumor'] @entity2592 :: ('MESH:D003561', 'Chemical') :: ['cytarabine']
@entity981 :: (MESH:D054198,Disease) :: ['Acute Lymphoblastic Leukemia']
