An outcrossed @entity235 pedigree was developed for quantitative trait locus (QTL) mapping of @entity572 by breeding dysplastic Labrador retrievers to trait-free greyhounds. Measured susceptibility traits included age at onset of femoral @entity9980 (OSS), maximum hip distraction (laxity) index ( @entity6684 ), and the dorsolateral subluxation (DLS) score. The pedigrees consisted of 147 @entity235 representing four generations. For 59 @entity235 genotyped with 65 microsatellites, the median heterozygosity and polymorphic information content (PIC) values of the F(1) generation were 0.82 and 0.68, respectively. Seventy-seven percent of microsatellites had a PIC greater than 0.59 in the F(1)s. Ninety-six percent of alleles showed Mendelian inheritance. Based on marker informativeness, approximately 350 randomly selected markers would be required for genome-wide screening to obtain an average interval between informative markers of 10 cM. Heritability was estimated as 0.43, 0.5, and 0.61 for OSS, @entity6684 , and the DLS score, respectively. Biometric estimates of the mean (+/- variance) effective number of segregating QTLs was 1.2 (+/- 0.05), 0.8 (+/- 0.02), and 1.0 (+/- 0.03) for OSS, @entity6684 , and the DLS score, respectively. The distributions of simulated backcross trait data suggested that the loci controlling these traits acted additively and that the @entity6684 may be controlled by a major locus. When combined with previous power and quantitative genetic analyses, these estimates indicate that this pedigree is informative for QTL mapping of @entity572 traits.

Genetic structure of susceptibility traits for XXXX and microsatellite informativeness of an outcrossed @entity235 pedigree.

@entity235 :: ('9615', 'Species') :: ['canine', 'dogs'] @entity6684 :: ('MESH:C564703', 'Disease') :: ['DI'] @entity572 :: ('MESH:D006617', 'Disease') :: ['hip dysplasia'] @entity9980 :: ('MESH:C562735', 'Disease') :: ['capital chondroepiphyseal ossification']
@entity572 :: (MESH:D006617,Disease) :: ['hip dysplasia']
