AIMS: To determine by fluorescence in situ hybridisation (FISH) whether deletion of D17S34, a subtelomeric probe for 17p, occurs in invasive squamous @entity189 , and to determine the extent of such loss by analysis of the @entity876 and @entity1722 genes. METHODS: Fourteen invasive @entity957 of the cervix were investigated by FISH for D17S34, @entity876 , and @entity1722 . Dual hybridisation of each probe with the chromosome 17 alpha satellite (D17Z1) probe was performed on paraffin wax embedded sections, and the fluorescence ratios of the paired signals were determined. Broad spectrum @entity1082 ) typing by ISH and GP5+/6+ polymerase chain reaction was also performed. RESULTS: Twelve @entity5 were HPV positive, nine with @entity1799 , and one each with types 18, 31, and 39. Loss of D17S34 was identified in four @entity5 , one of which was HPV negative. In one @entity5 , D17S34 loss was accompanied by loss of @entity876 only, suggesting that deletion was limited to the p arm. A second @entity5 showed simultaneous losses of all probes, indicative of whole chromosome 17 loss during @entity5 growth. The two remaining specimens showed loss of D17S34 only, diffuse in one, and localised within the @entity5 in the other. Aberrations of @entity876 or @entity1722 were not seen independently of D17S34 loss, and loss did not correlate with HPV presence or type. CONCLUSIONS: These data show that D17S34 loss is prevalent, marking 28% of the invasive @entity957 in this study. The observed intratumoral heterogeneity indicates that, at least in some cases, this loss occurs after invasion and is therefore a late event in the path of cervical @entity105 .

Loss of sequences on the short arm of chromosome 17 is a late event in squamous XXXX .

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumours', 'tumour'] @entity957 :: ('MESH:D002294', 'Disease') :: ['squamous carcinomas', 'squamous cell carcinomas'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinoma of the cervix'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity1799 :: ('333760', 'Species') :: ['HPV-16'] @entity1082 :: ('10566', 'Species') :: ['human papillomavirus (HPV'] @entity105 :: ('MESH:D063646', 'Disease') :: ['carcinogenesis'] @entity1722 :: ('2064', 'Gene') :: ['HER2/NEU']
@entity189 :: (MESH:D002277,Disease) :: ['carcinoma of the cervix']
