Among bioassays for evaluating various impacts of chemicals on @entity1 and ecosystems, those based on cultured @entity1 -cells can best predict acute lethal @entity137 to @entity1 . We expect them to be employed in the future in environmental risk management alongside mutagenicity tests and endocrine-disrupting activity tests. We recently developed a disposable bioassay device that immobilizes @entity1 hepatocarcinoma cells in a small micropipette tip. This enables very quick (within 2 h) evaluation of acute lethal @entity137 to @entity1 . For bioassay-based environmental management, 2 promising approaches have been demonstrated by the US-EPA: @entity137 identification evaluation (TIE) and @entity137 reduction evaluation (TRE). The Japanese Ministry of Environment has been supporting a multi-center validation project, aimed at assembling a bioassay database. To make full use of these resources, we present a numerical model that describes contribution of individual chemical to observed @entity137 . This will allow the selection of the most effective countermeasure to reduce the @entity137 . Bioassay-based environmental risk management works retrospectively, whereas impact assessment using substance flow models and @entity137 databases works prospective. We expect that these 2 approaches will exchange information, act complementarily, and work effectively in keeping our environment healthy in the 21 st century.

Cultured XXXX -cell-based bioassay for environmental risk management.

@entity1 :: ('9606', 'Species') :: ['humans', 'mammalian', 'human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity']
@entity1 :: (9606,Species) :: ['human']
