Intestinal @entity3960 ( @entity3960 ) produce the fibrotic tissue, strictures, that characterize @entity30 . These @entity3960 change their phenotype from a contractile muscle form to an @entity32 -responsive form that migrates and synthesizes a collagen matrix. It is postulated that the inflammatory responsive @entity3960 form associates differently with its surrounding collagen matrix compared to the normal @entity3960 form. @entity3960 derived from Crohn's diseased and uninvolved bowel were sustained in cell culture. Cultured @entity3960 incorporated in collagen lattices have the capacity to reduce the size of that lattice, referred to as lattice contraction. At day 2, @entity30 -populated collagen lattices were reduced to 21% of their initial area, while non- @entity30 collagen lattices were reduced to 8%. @entity30 demonstrate retarded lattice contraction compared to non- @entity30 . When grown in monolayer culture, Crohn's-derived @entity3960 cover 30% more area than non- @entity30 . By Western blot analysis @entity30 express more @entity19330 , an actin-binding protein found elevated in cells exhibiting increased cell motility. Was the increased expression of @entity19330 related to retarded collagen lattice contraction? Intracellular levels of @entity19330 were elevated by the electroporation of plasma @entity19330 protein into suspended non- @entity30 . When incorporated in collagen lattices, @entity19330 loaded cells showed retarded lattice contraction compared to @entity3960 loaded with albumin. @entity30 show increased expression of @entity19330 , which may be associated with a diminished capacity to reorganize collagen fiber bundles. It is suggested that increased concentrations of @entity19330 in @entity30 is consistent with enhanced cell migration as a consequence of the inflammatory state of Crohn's diseased intestine.

Increased @entity19330 expression and retarded collagen lattice contraction with XXXX from Crohn's diseased intestine.

@entity19330 :: ('2934', 'Gene') :: ['gelsolin'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity3960 :: ('MESH:D018235', 'Disease') :: ['smooth muscle cells', 'SMC'] @entity30 :: ('MESH:D003424', 'Disease') :: ["Crohn's SMC", "Crohn's disease"]
@entity3960 :: (MESH:D018235,Disease) :: ['smooth muscle cells']
