UNASSIGNED: Huo Luo Xiao Ling Dan (HLXLD), a Chinese herbal formula, is used in folk medicine for the treatment of @entity625 and other chronic inflammatory diseases. However, the in vivo integrated metabolism of its multiple components remains unknown. In this paper, an ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS) method was developed for detection and identification of HLXLD metabolites in @entity35 urine at high and normal clinical dosages. The prototype constituents and their metabolites in urine were analyzed. The mass measurements were accurate within 8 ppm, and subsequent fragment ions offered higher quality structural information for interpretation of the fragmentation pathways of various compounds. A total of 85 compounds were detected in high dosages urine samples by a highly sensitive extracted ion chromatograms method, including 31 parent compounds and 54 metabolites. Our results indicated that phase 2 reactions (e.g. glucuronidation, glutathionidation and sulfation) were the main metabolic pathways of @entity18201 , alkaloids and @entity4212 , while phase I reactions (e.g. hydrogenation and hydroxylation) were the major metabolic reaction for @entity6397 , @entity13575 and @entity68346 . This investigation provided important structural information on the metabolism of HLXLD and provided scientific evidence to obtain a more comprehensive metabolic profile. Copyright    2015 John Wiley _ Sons, Ltd.

Metabolites identification of Huo Luo Xiao Ling Dan in XXXX urine by UPLC coupled with electrospray ionization time-of-flight mass spectrometry.

@entity18201 :: ('MESH:D007783', 'Chemical') :: ['lactones'] @entity625 :: ('MESH:D001168', 'Disease') :: ['arthritis'] @entity4212 :: ('MESH:D047309', 'Chemical') :: ['flavones'] @entity6397 :: ('MESH:D003374', 'Chemical') :: ['coumarins'] @entity13575 :: ('MESH:C015423', 'Chemical') :: ['paeoniflorin'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity68346 :: ('MESH:D039823', 'Chemical') :: ['iridoids']
@entity35 :: (10116,Species) :: ['rat']
