Cultured @entity291 aortic endothelial cells were fixed and stained with @entity68888 and quantitated with a digital image analyzer for changes in actin cables and surface area. @entity750 ( @entity750 ), @entity1063 (NE), @entity935 and @entity112 (all at 10(-4)M concentrations) were tested for their ability to induce cytoskeletal changes. Only @entity750 and NE increased actin cables significantly (p less than 0.01), 80.7% and 97.9%, respectively. @entity935 and @entity112 treated cells showed a 67.4% and 80.8% decrease in actin cables respectively (p less than 0.01). Stimulated increases of actin cables by @entity750 were inhibited by @entity6009 , and @entity1169 inhibited NE stimulation of actin cables. Treatment of cells with these blockers alone also decreased actin cables below control values (p less than 0.01). Pretreatment of cells with @entity3933 , but not @entity4444 , inhibited @entity112 -induced decreases in actin cables. Stimulation of surface area by @entity750 and NE was also observed, with 40.8% and 80.7% increases respectively, when compared with controls (p less than 0.01). The increases in actin cables were associated with a lack of ruffled edges that are indicative of motile cells. In contrast, induced decreases in actin cables resulted in cells with ruffled edges. Exogenous @entity750 and NE have been shown to prevent the increased permeability visible as extravasation of red blood cells from postcapillary venules in @entity882 animals. The present data suggest that @entity750 and NE may be involved in maintaining the endothelial barrier function by a receptor-mediated stimulation of actin cables. Also, @entity112 -induced decreases in actin cables may be correlated with the @entity6115 's action in vivo as a mediator of increased inflammatory permeability.

Vasoactive amines modulate actin cables (stress fibers) and surface area in cultured XXXX endothelium.

@entity1169 :: ('MESH:D011433', 'Chemical') :: ['propranolol'] @entity4444 :: ('MESH:D002927', 'Chemical') :: ['cimetidine'] @entity3933 :: ('MESH:D004155', 'Chemical') :: ['diphenhydramine'] @entity291 :: ('9913', 'Species') :: ['bovine'] @entity882 :: ('MESH:D013921', 'Disease') :: ['thrombocytopenic'] @entity935 :: ('MESH:D004298', 'Chemical') :: ['dopamine', 'Dopamine'] @entity6115 :: ('CHEBI:32952', 'Chemical') :: ['amine'] @entity750 :: ('MESH:D012701', 'Chemical') :: ['Serotonin', '5-HT'] @entity112 :: ('MESH:D006632', 'Chemical') :: ['histamine'] @entity6009 :: ('MESH:D007650', 'Chemical') :: ['Ketanserin'] @entity1063 :: ('MESH:D009638', 'Chemical') :: ['norepinephrine'] @entity68888 :: ('MESH:C028877', 'Chemical') :: ['NBD-phallicidin']
@entity291 :: (9913,Species) :: ['bovine']
