PURPOSE: Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) play important roles in the @entity32 in @entity66 models. However, their role in vein graft (VG) remodeling is undefined. We aimed to investigate the effect of EVs from @entity1958 MSCs (ADMSC-EVs) on VG intimal @entity1373 and to explore the possible mechanisms. METHODS: After generation and characterization of control-EVs and ADMSC-EVs in vitro, we investigated their effect on the proliferation and migration of vascular smooth muscle cells (VSMCs) in vitro. Next, we established a @entity19 model of VG transplantation. @entity19 underwent surgery and received control-EVs or ADMSC-EVs by intraperitoneal injection every other day for 20  days. VG remodeling was evaluated after 4  weeks. We also assessed the effect of ADMSC-EVs on macrophage migration and inflammatory cytokine expression. RESULTS: Significant inhibitory effects of ADMSC-EVs on in vitro VSMC proliferation (p   <   0.05) and migration (p   <   0.05) were observed compared with control-EVs. The extent of intimal @entity1373 was significantly decreased in ADMSC-EV-treated @entity19 compared with control-EV-treated @entity19 (26        8.4 vs. 45        9.0   m, p   <   0.05). A reduced presence of macrophages was observed in ADMSC-EV-treated @entity19 (p   <   0.05). Significantly decreased expression of inflammatory cytokines @entity777 and @entity1750 ( @entity1750 ) was also found in the ADMSC-EV-treated group (both p   <   0.05). In addition, phosphorylation of @entity1994 , @entity8837 , and @entity1995 in VGs was decreased in the ADMSC-EV-treated group. CONCLUSIONS: We demonstrated that ADMSC-EVs exert an inhibitory effect on VG @entity8233 formation by regulating VSMC proliferation and migration, macrophage migration, inflammatory cytokine expression, and the related signaling pathways.

Extracellular Vesicles Derived from @entity1958 Mesenchymal Stem Cells Regulate the Phenotype of Smooth Muscle Cells to Limit Intimal XXXX .

@entity32 :: ('MESH:D007249', 'Disease') :: ['reduction of inflammation'] @entity66 :: ('MESH:D004194', 'Disease') :: ['multiple disease'] @entity1750 :: ('20296(Tax:10090)', 'Gene') :: ['monocyte chemoattractant protein-1', 'MCP-1'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'Mice'] @entity1373 :: ('MESH:D006965', 'Disease') :: ['hyperplasia'] @entity1958 :: ('230796(Tax:10090)', 'Gene') :: ['adipose'] @entity777 :: ('16193(Tax:10090)', 'Gene') :: ['interleukin (IL)-6'] @entity1994 :: ('11651(Tax:10090)', 'Gene') :: ['Akt'] @entity1995 :: ('26416(Tax:10090)', 'Gene') :: ['p38'] @entity8837 :: ('26417;26413', 'Gene') :: ['Erk1/2'] @entity8233 :: ('MESH:D058426', 'Disease') :: ['neointima']
@entity1373 :: (MESH:D006965,Disease) :: ['Hyperplasia']
