OBJECTIVE: To investigate the effects of shRNA targeting @entity8207 on tumorigenicity of @entity1 @entity3052 K562 cells in @entity19 in vivo and its mechanisms. METHODS: For experiment 3 groups including @entity573 group(expression of @entity8207 in K562 cells was down-regulated by shRNA), CON (cells without any treatment) and NC group (cells treated with nonspecific-sequence shRNA) were set-up. Western blot was used to test the expression of b-catenin and @entity1896 in cells. Then the cells of 3 groups were implanted into BALB/c @entity19 subcutaneously to establish @entity19 xenograft model. The growth state of the @entity19 and the size of the xenograft @entity5 were recorded. HE staining was used to observe the morphology of xenograft @entity5 . Expressions of b-catenin and @entity1896 in xenograft @entity5 were detected by immunohistochemical staining. RESULTS: In vitro the expression of b-catenin and @entity1896 in @entity573 group were lower than the CON group and NC group (P<0.05). In vivo the @entity5 volume and weight of @entity573 group were significant smaller than the other two groups (P<0.01). HE staining showed that tissues in the @entity573 group were relatively looser in arrangement with smaller cell nucleus and less cytoplasm. The expression of b-catenin and @entity1896 in the @entity573 group were remarkable weak as compared with that in CON group and NC group (P<0.05). CONCLUSION: Down-regulating the expression of @entity8207 by shRNA may significantly inhibit the tumorigenicity of K562 cells in @entity19 in vivo and its mechanism may be related with the inhibition of expression of b-catenin and @entity1896 .

[Effects of shRNA Targeting @entity8207 on Tumorigenicity of K562 Cells in XXXX In Vivo].

@entity1 :: ('9606', 'Species') :: ['human'] @entity1896 :: ('12443(Tax:10090)', 'Gene') :: ['cyclinD1'] @entity573 :: ('MESH:C537014', 'Disease') :: ['KD'] @entity19 :: ('10090', 'Species') :: ['mice', 'nude mice', 'murine'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity3052 :: ('MESH:D015470', 'Disease') :: ['acute leukemia'] @entity8207 :: ('64292(Tax:10090)', 'Gene') :: ['mPGES-1']
@entity19 :: (10090,Species) :: ['Nude Mice']
