UNASSIGNED: BACKGROUND As a traditional Chinese medicine herb, Chonglou (Paris polyphylla var. chensiins) has been used as anticancer medicine in China in recent decades, as it can induce cell cycle arrest and apoptosis in numerous @entity5 cells. The saponins extract from the rhizoma of Chonglou [Rhizoma Paridis saponins (RPS)] is known as the main active component for anticancer treatment. However, the molecular mechanism of the anticancer effect of RPS is unknown. MATERIAL AND METHODS The present study evaluated the effect of RPS in @entity1149 ( @entity1149 ) A549 cells using the 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide (MTT) assay and flow cytometry. Subsequently, the expression of several genes associated with cell cycle and apoptosis were detected by reverse transcription-quantitative polymerase chain reaction (qRT-PCR) and Western blotting. RESULTS RPS was revealed to inhibit cell growth, causing a number of cells to accumulate in the G 1 phase of the cell cycle, leading to apoptosis. In addition, the effect was dose-dependent. Moreover, the results of qRT-PCR and Western blotting showed that @entity876 and @entity15308 ( @entity15308 ) were significantly downregulated, and that @entity588 , @entity589 , and @entity994 were upregulated, by RPS treatment. CONCLUSIONS We speculated that the RPS could act on a pathway, including @entity876 , @entity994 , @entity588 , @entity589 , and @entity15308 , and results in G1 cell cycle arrest and apoptosis in @entity1149 cells.

Rhizoma Paridis Saponins Induces Cell Cycle Arrest and Apoptosis in XXXX A549 Cells.

@entity589 :: ('581', 'Gene') :: ['BAX'] @entity588 :: ('596', 'Gene') :: ['BCL2'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity1149 :: ('MESH:D002289', 'Disease') :: ['NSCLC', 'non-small-cell lung cancer'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity15308 :: ('1017', 'Gene') :: ['cyclin-dependent kinase 2', 'CDK2'] @entity994 :: ('1026', 'Gene') :: ['p21']
@entity1149 :: (MESH:D002289,Disease) :: ['Non-Small Cell Lung Carcinoma']
