@entity3001 often presents initially with @entity8831 . Borreliae may disseminate from the primary @entity272 , and different organs and systems could be affected. Borrelia strains were isolated from blood of 70 @entity1 with @entity3001 , including 10 @entity1 from whom borreliae were also isolated from skin. The aim of the present study was to characterise the isolates with regard to their phenotypic and genotypic characteristics. Borreliae were cultivated in MKP medium. Species identification and plasmid profiles were determined by pulsed-field gel electrophoresis (PFGE) and protein profiles by SDS-PAGE. Digestion of @entity2069 sensu lato DNA showed 63 (90%) @entity21786 Mla1 and 7 (10%) @entity25974 Mlg2. No @entity2069 were isolated. Borreliae were isolated from both skin and blood of 10 @entity1 , nine pairs of isolates were identical: seven @entity21786 and two @entity25974 . @entity21786 was isolated from the skin and @entity25974 from blood of the tenth @entity1 . All but one isolate possessed at least one large plasmid and varying numbers of smaller plasmids. Eight (11.4%) of 70 isolates possessed an unusual plasmid profile (2 of 63 @entity21786 and 6 of 7 @entity25974 ). Borreliae differed in their protein profiles. OspA and OspB proteins were expressed by all @entity21786 isolates; 85.7% of @entity25974 isolates expressed OspA and 71.4% expressed OspB. OspC was expressed by 65% of @entity21786 isolates and all @entity25974 isolates. The ratios of @entity21786 and @entity25974 isolated from blood and skin were similar. These results do not support the hypothesis that @entity25974 has a higher propensity for haematogenous dissemination than B. afzelii. Antigen diversity as well as species and plasmid heterogeneity could play a role in the pathogenesis of the infection, suggesting distinctive strain organotropism.

Genotypic and phenotypic characterisation of XXXX sensu lato strains isolated from @entity1 blood.

@entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity3001 :: ('MESH:D008193', 'Disease') :: ['Lyme borreliosis'] @entity21786 :: ('29518', 'Species') :: ['B. afzelii'] @entity2069 :: ('139', 'Species') :: ['B. burgdorferi sensu stricto', 'Borrelia burgdorferi'] @entity8831 :: ('MESH:C538499', 'Disease') :: ['erythema migrans'] @entity272 :: ('MESH:D012871', 'Disease') :: ['skin lesion'] @entity25974 :: ('29519', 'Species') :: ['B. garinii']
@entity2069 :: (139,Species) :: ['Borrelia burgdorferi']
