OBJECTIVE: To explore the effects of antisense oligodeoxynucleotides (ASODN) on proliferation and apoptosis in @entity356 cell line @entity1 cells and the molecular mechanisms induced by ASODN. METHODS: survivin ASODN-1, survivin ASODN-2 and survivin ASODN-3 were transfected into @entity1 cells by Lipofectamine(TM) 2000 transfection reagent. The growth activity of @entity1 cells was detected by @entity98 assay. Apoptosis index (AI), proliferation index (PI), cell cycle and expressions of survivin, @entity7 and @entity17205 / @entity17205 proteins were detected by flow cytometry (FCM). The changes of survivin mRNA, @entity7 mRNA and @entity17205 / @entity17205 mRNA were detected by RT-PCR. RESULTS: The expression of survivin was down-regulated by the three ASODN sequences, especially the ASODN-2 was best. At 48 hours after transfection with 600 nmol/L survivin ASODN-2, the cells in G(1)/G(0) phase were significantly increased [(72.25    2.95)%], apoptotic index increased [(11.31    0.38)%], proliferation index decreased [(27.77    2.97)%], compared with those in the control group [(56.25    0.75)%, (1.62    0.36)%, (43.80    0.80)%, all P < 0.05]. The survivin mRNA and protein levels (0.523    0.091, 0.733    0.009) were down-regulated compared with those in the control group (0.861    0.047, 0.997    0.233), @entity7 (0.519    0.076, 0.75    0.006) were down-regulated compared with those in the control group (0.779    0.059, 1.000    0.01), while those of @entity17205 / @entity17205 (0.899    0.113, 1.637    0.023)were up-regulated compared with those in the control group (0.558    0.041, 1.000    0.049, all P < 0.05). CONCLUSIONS: Survivin ASODN can induce apoptosis and inhibit the proliferation of @entity356 cell line @entity1 cells. Those effects are induced through up-regulation of @entity17205 / @entity17205 and down-regulation of survivin and @entity7 expression simultaneously.

[Effect of antisurvivin oligodeoxynucleotides on proliferation and apoptosis in XXXX cell line BGC-823 and the molecular mechanism].

@entity1 :: ('9606', 'Species') :: ['BGC-823'] @entity17205 :: ('56616', 'Gene') :: ['DIABLO', 'Smac'] @entity7 :: ('7422', 'Gene') :: ['VEGF'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity356 :: ('MESH:D013274', 'Disease') :: ['gastric cancer']
@entity356 :: (MESH:D013274,Disease) :: ['gastric cancer']
