In order to obtain more information on the in vitro transformation of @entity1 cells, a @entity1 fetal tracheal epithelial cell line (FHET16/5) was exposed for a long time to @entity107 ( @entity108 ). In 20 passages, this cell line (diploid, male) maintained strong immunohistochemical reactivity for carcino-embryonic antigen and wool merokeratin; it was negative for @entity2277 . The cells contained PAS-positive mucous substances and ultrastructurally were found to have desmosome-like attachments. Treatment of the cells was with 0.3% @entity3733 ( @entity3733 ), or @entity3733 with 150, 450, 1000 or 2000 micrograms/ml of @entity108 . It was started at the ninth passage and continued for six passages over 9 weeks for the control ( @entity3733 ) and the three lowest control doses of @entity108 , and for three passages over 9 weeks for the 2000 micrograms/ml @entity108 group. Cells grown for 13 days after the end of treatment were plated in soft agar and injected subcutaneously in @entity19 . The frequency of anchorage-independent colonies grown in soft agar was directly related to @entity108 dose. Colony-forming efficiency, as an expression of toxic effect, was also dose dependent. Autoradiographically detected unscheduled DNA synthesis indicated an association between anchorage-independent transformation and DNA alterations induced by @entity108 . Cells injected into @entity19 did not produce @entity5 during a 6-month period, but invasiveness was observed when cells from the 2000 micrograms/ml @entity108 group were transplanted on the dermis of cultured @entity1573 embryo skin. The results indicate that @entity108 causes anchorage-independent transformation accompanied by unscheduled DNA synthesis in a fetal @entity1 tracheal epithelial cell line.

Growth inhibition and transformation of a @entity1 fetal tracheal epithelial cell line by long-term exposure to XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity108 :: ('CHEBI:34873', 'Chemical') :: ['DEN'] @entity19 :: ('10090', 'Species') :: ['nude mice'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumours'] @entity107 :: ('MESH:D004052', 'Chemical') :: ['diethylnitrosamine'] @entity3733 :: ('MESH:D004121', 'Chemical') :: ['dimethyl sulphoxide', 'DMSO'] @entity1573 :: ('9031', 'Species') :: ['chick'] @entity2277 :: ('7431', 'Gene') :: ['vimentin']
@entity107 :: (MESH:D004052,Chemical) :: ['diethylnitrosamine']
