@entity3928 ( @entity3928 ) coupled with 1-D and 2-D electrophoresis can be utilized to detect and identify immunogenic proteins, but these methods are laborious and time-consuming. We describe an alternative, simple, rapid gel-free strategy to identify multiple immunogenic proteins from @entity7320 (Bp). It couples immunoprecipitation to nano liquid chromatography- tandem mass spectrometry (IP-nLC- @entity3928 / @entity3928 ) and is significantly both time- and labor-saving. We developed a gel-free magnetic bead-based immunoprecipitation (IP) method using different @entity10385 /PBS concentrations in which solubilized proteins of Bp Tohama I membrane fractions were precipitated with polyclonal rabbit anti-Bp whole cell immune sera. Immune complexes were analyzed by @entity3928 and Scaffold analysis (>95% protein identification probability). Total immunoproteins identified were 50, 63 and 49 for 0.90%, 0.45% and 0.22% @entity10385 /PBS buffer concentrations respectively. Known Bp proteins identified included pertactin, serotype 2 fimbrial subunit and filamentous hemagglutinin. As proof of concept that this gel-free protein immunoprecipitation method enabled the capture of multiple immunogenic proteins, IP samples were also analyzed by @entity214 -PAGE and immunoblotting. Bypassing gels and subjecting immunoprecipitated proteins directly to @entity3928 is a simple and rapid antigen identification method with relatively high throughput. IP-nLC- @entity3928 / @entity3928 provides a novel alternative approach for current methods used for the identification of immunogenic proteins.

A rapid method for capture and identification of immunogenic proteins in XXXX enriched membranes fractions: a fast-track strategy applicable to other microorganisms.

@entity7320 :: ('520', 'Species') :: ['Bordetella pertussis'] @entity3928 :: ('MESH:C536030', 'Disease') :: ['Mass spectrometry', 'MS'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS'] @entity10385 :: ('MESH:C010615', 'Chemical') :: ['NP-40']
@entity7320 :: (520,Species) :: ['Bordetella pertussis']
