The aim of this study was to analyse chromosomally @entity21562 -resistant @entity526 isolates for their genetic relatedness, and also for the presence of the @entity64597 gene and its adjacent regions, in order to compare these regions with those associated with a @entity64597 gene located on a conjugative plasmid from @entity526 . Twenty-two @entity291 @entity526 from France and Germany were examined. @entity21562 resistance was determined by MIC determination. The presence of the @entity64597 gene was confirmed by hybridization and PCR analysis. The @entity526 isolates were investigated by macrorestriction analysis. The 22 @entity21562 -resistant @entity526 (MICs 64->128 mg/L) differed in their BlnI macrorestriction patterns. Single or double copies of the @entity64597 gene were detected by hybridization on different-sized chromosomal EcoRI, BamHI and BglI fragments. The @entity64597 -flanking regions also proved to be variable as confirmed by hybridization experiments. The detection of chromosomal @entity64597 gene copies in unrelated @entity526 isolates supplements the observations of @entity64597 genes on plasmids in @entity526 and confirms their potential to integrate into the chromosome. The RFLPs of @entity64597 gene-carrying restriction fragments might suggest variable chromosomal integration sites.

Molecular analysis of chromosomally XXXX -resistant @entity526 isolates from France and Germany.

@entity21562 :: ('MESH:C035534', 'Chemical') :: ['Florfenicol', 'florfenicol'] @entity291 :: ('9913', 'Species') :: ['bovine'] @entity526 :: ('562', 'Species') :: ['Escherichia coli', 'E. coli'] @entity64597 :: ('13905643', 'Gene') :: ['floR']
@entity21562 :: (MESH:C035534,Chemical) :: ['florfenicol']
