The regulation of phenotype and function of @entity1 @entity409 ( @entity409 ) by in vitro culture with @entity1199 , IL-12 and anti-CD3 monoclonal antibodies (mAb) was investigated. The CD3+ @entity409 which express 50% less CD3 molecules per cell than peripheral blood T lymphocytes, exhibited a 6-fold reduction in proliferation when stimulated through the CD3 complex by anti-CD3 mAb. @entity409 freshly isolated or cultured in medium did not suppress MLR response, nor were they cytotoxic. However, treatment of the @entity409 cells with @entity1199 , IL-12 and anti-CD3 induced these cells to suppress autologous responding cells in MLR (ca. 70%) and to kill autologous or allogeneic cells. Low level @entity137 could be induced by cytokines @entity1199 , IL-12 or anti-CD3 alone. However, the development of optimum MLR suppression and @entity137 induction was dependent upon stimulation of the @entity409 cells through the CD3 complex. The co-expression of CD3 and @entity2940 on @entity409 was also up-regulated by anti-CD3 stimulation in the combination of @entity1199 and IL-12. Most of the CD3+/ @entity2940 + cells, also expressed @entity405 . After the magnetic bead separation procedure, the cytotoxic activity was found mainly in the CD3+/ @entity2940 +/ @entity405 + population. These results suggest that CD3+/ @entity2940 +/ @entity405 + cells can be expanded by stimulation through the TCR/CD3 complex in the presence of @entity1199 and IL-12, which results in the suppression of autologous responding cells by a cytotoxic mechanism. The proliferative response of the CD3+/ @entity2940 +/ @entity405 + population was enhanced by the induction of @entity4897 molecules on the stimulating cells, and anti- @entity4897 mAb were able to block the response in a dose-dependent manner. The CD3+/ @entity2940 +/ @entity405 + cells were examined for cytokine production by flow cytometry. Cytokines @entity3646 , @entity310 , and @entity379 were produced by 91.7%, 29.2%, and 27.4% of the cells, respectively.

The regulation of phenotype and function of XXXX liver CD3+/ @entity2940 + lymphocytes, and cells that also co-express @entity405 by @entity1199 , IL-12 and anti-CD3 monoclonal antibody.

@entity1 :: ('9606', 'Species') :: ['human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity2940 :: ('4684', 'Gene') :: ['CD56'] @entity310 :: ('7124', 'Gene') :: ['TNF-alpha'] @entity409 :: ('MESH:D008107', 'Disease') :: ['liver infiltrating lymphocytes', 'LIL'] @entity4897 :: ('911', 'Gene') :: ['CD1'] @entity379 :: ('3458', 'Gene') :: ['IFN-gamma'] @entity1199 :: ('3558', 'Gene') :: ['IL-2'] @entity3646 :: ('3565', 'Gene') :: ['IL-4'] @entity405 :: ('925', 'Gene') :: ['CD8']
@entity1 :: (9606,Species) :: ['human']
