@entity68100 is a component of eukaryotic chromatin whose expression has not been studied in plants. We isolated and characterized a @entity523 and a @entity3587 cDNA encoding @entity68100 . We found that in @entity523 H2A is encoded by a small gene family and that both the @entity3587 and the @entity523 mRNAs are polyadenylated. @entity523 H2A has 82% @entity535 residue identity to @entity3587 H2A, 83% to @entity3505 , and 65% to @entity1 and @entity375 H2A. Plant H2As differ from fungal and animal H2As in their @entity1472 -terminal and @entity1473 -terminal regions. @entity1473 -terminal plant H2A regions contain the motif SPKK, a peptide implicated in binding of A/T-rich DNA regions. By using RNA gel blot analysis, we determined that the steady-state mRNA level of these genes was abundant in apices and early developing fruit and very low in mature tissues. In situ RNA hybridization showed strong spatial regulation because the mRNA was abundant in some cells and not detectable in others. In @entity523 shoot tips, H2A-expressing cells were distributed irregularly in or near meristems. In @entity523 or @entity3587 root tips, expressing cells were concentrated near the apex, and their distribution was consistent with that expected of cycling cells. Other H2A transcripts were found in nondividing cortical cells that are known to undergo endoduplication during the late maturation phase of primary development.

Cell-specific expression of plant XXXX genes.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1472 :: ('CHEBI:46882', 'Chemical') :: ['amino'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity523 :: ('4081', 'Species') :: ['tomato', 'Tomato'] @entity3505 :: ('4565', 'Species') :: ['wheat'] @entity375 :: ('4932', 'Species') :: ['yeast'] @entity68100 :: ('101265996', 'Gene') :: ['Histone H2A', 'histone H2A'] @entity3587 :: ('3888', 'Species') :: ['pea'] @entity1473 :: ('CHEBI:46883', 'Chemical') :: ['carboxy', 'Carboxy']
@entity68100 :: (101265996,Gene) :: ['histone H2A']
