The aim of this study was to determine the optimal conditions (effect of culture time before and after cryopreservation) for cryopreservation of specific pathogen-free @entity1707 islet cells. METHODS: (1) @entity413 -induced @entity11938 secretion by fresh islet cells cultured for 10 days was compared to that by islet cells cryopreserved 7 days after isolation and cultured 3 days after thawing. (2) Islet cells were cryopreserved 1, 7, or 14 days after isolation and cultured 3, 7, 14, or 21 days after thawing. Islet cell number, @entity11938 content, and @entity11938 response under perifusion tests were investigated. RESULTS: (1) @entity11938 response by cryopreserved islet cells was identical to that by fresh islet cells (basal/stimulation index: 2. 13 +/- 0.19 vs 2.17 +/- 0.16, n = 4, NS), although the amount of secreted @entity11938 was reduced by 40% (area under the curve: 2136 +/- 198 pM/10(4) cells/180 min vs 3564 +/- 636 pM/10(4) cells/180 min, P = 0.104). (2) Cell number 6 days after thawing was reduced by 54, 40, and 63% when cryopreservations were carried out at D1, D7, and D14. (3) @entity11938 content in cultured or cryopreserved islet cells increased between 7 and 14 days of culture. (4) Whatever the culture time before and after cryopreservation, @entity11938 secretion in response to @entity413 was maintained. The @entity11938 release was the highest for islet cells cryopreserved 14 days after isolation and cultured 14 days after thawing (stimulation index: 6.19 +/- 2.68). CONCLUSIONS: SPF @entity1707 islet cells remained functional after cryopreservation in @entity968 and it may be important to culture islet cells over 14 days before and after cryopreservation.

Cryopreservation of specific pathogen-free (SPF) XXXX islet cells: effect of culture time before cryopreservation and after thawing.

@entity1707 :: ('9823', 'Species') :: ['pig'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose', 'Glucose'] @entity968 :: ('MESH:D011092', 'Chemical') :: ['polyethylene glycol'] @entity11938 :: ('397415(Tax:9823)', 'Gene') :: ['insulin', 'Insulin']
@entity1707 :: (9823,Species) :: ['pig']
