BACKGROUND: We previously identified MGL_1304 secreted by @entity27220 as a sweat antigen for @entity1 with @entity483 ( @entity483 ) and cholinergic @entity110 (ChU). However, purifying native MGL_1304 from @entity1 sweat or culture supernatant of @entity27220 (sup-MGL_1304) is costly and time-consuming. Moreover, recombinant MGL_1304 expressed by using @entity526 (TF-rMGL_1304) needs a large chaperon protein and lacks the original glycosylation of @entity375 . Thus, we generated a recombinant MGL_1304 by @entity10056 (P-rMGL_1304) and investigated its characteristic features. METHODS: Recombinant MGL_1304 proteins expressed by @entity526 and @entity10056 were generated. Properties of these recombinants and native antigens were compared by western blot analysis, @entity112 release tests (HRT) of @entity1 with @entity483 and ChU, and b-hexosaminidase release tests with RBL-48 cells. P-rMGL_1304-specific IgE in sera of @entity1 with @entity483 were measured by sandwich ELISA. RESULTS: Western blot analysis revealed that IgE of @entity1 with @entity483 bound to all MGL_1304 recombinants and native antigens. The @entity112 releasing ability of P-rMGL_1304 was 100 times higher than that of TF-rMGL_1304, and was comparable to that of sup-MGL_1304. Degranulation rates of RBL-48 cells, sensitized with sera of @entity1 with @entity483 in response to the stimulation of P-rMGL_1304, were comparable to those of sup-MGL_1304, whereas those of TF-rMGL_1304 were relatively weak. The levels of P-rMGL_1304-specific IgE in sera of @entity1 with @entity483 were correlated with their disease severities. CONCLUSIONS: P-rMGL_1304 has an antigenicity comparable to the native antigen, and is more useful than TF-rMGL_1304, especially in HRT and degranulation assay of RBL-48 cells.

Evaluation of recombinant MGL_1304 produced by XXXX for clinical application to sweat allergy.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity483 :: ('MESH:D003876', 'Disease') :: ['atopic dermatitis', 'AD'] @entity526 :: ('562', 'Species') :: ['Escherichia coli', 'E.  coli'] @entity10056 :: ('4922', 'Species') :: ['Pichia pastoris', 'P.  pastoris'] @entity27220 :: ('76773', 'Species') :: ['M.  globosa', 'Malassezia globosa'] @entity112 :: ('MESH:D006632', 'Chemical') :: ['histamine'] @entity375 :: ('4932', 'Species') :: ['yeasts'] @entity110 :: ('MESH:D014581', 'Disease') :: ['urticaria']
@entity10056 :: (4922,Species) :: ['Pichia pastoris']
