Background/Aim: Nucleic acid metabolism is biochemically compartmentalized to the nucleus. Thus, it is necessary to define the proteome of the various macromolecular structures within this organelle. Materials and Methods: We isolated the nuclear matrix (NM) fraction from @entity35 liver by sequential centrifugation steps at 13,000 rpm, staggered between endogenous nuclease treatment for 2 h at 37  C, followed by high-salt (H.S.; 2.0 M @entity1219 ) and non-ionic detergent extractions (0.1%- or 1.0% @entity6000 ) to eliminate the bulk of chromosomal DNA/RNA, histone proteins and the nuclear envelope (NE). Results: Integrity of the NM and NE structures was confirmed by electron microscopy. Next, we analyzed the NM proteome on a 20% @entity2975 gel using the PhastSystem. We observed the absence of histone proteins and the characteristic presence of the lamins by Coomassie blue staining. By contrast, upon @entity575 staining, following electrophoretic separation with a Tris-Borate- @entity1098 buffer, we observed the NM-associated nucleic RNA and protein-free @entity857 - @entity8690 polymers. While polymers are found in much lower concentration than RNA in NM, they were purified by affinity chromatography on @entity15841 resin prior to electrophoresis. We observed the electrophoretic resolution of free @entity857 - @entity8690 chains (5-25 units) by @entity575 staining. Conclusion: The significance of our observations to @entity5 studies and @entity105 is discussed.

Electrophoretic Characterization of the Mammalian Nuclear Matrix Proteome, Nuclear Envelope, Nucleoli and Covalently Bound ADP-Ribose Polymers: Potential Applications to XXXX .

@entity6000 :: ('MESH:D017830', 'Chemical') :: ['Triton X-100'] @entity575 :: ('MESH:D012834', 'Chemical') :: ['silver'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity1219 :: ('CHEBI:26710', 'Chemical') :: ['NaCl'] @entity15841 :: ('CHEBI:50978', 'Chemical') :: ['boronate'] @entity8690 :: ('MESH:D012266', 'Chemical') :: ['ribose'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity857 :: ('MESH:D000244', 'Chemical') :: ['ADP'] @entity105 :: ('MESH:D063646', 'Disease') :: ['carcinogenesis'] @entity1098 :: ('MESH:D004492', 'Chemical') :: ['EDTA']
@entity5 :: (MESH:D009369,Disease) :: ['Cancer']
