Internalisation and recycling of seven trans-membrane domain receptors is a critical regulatory event for their signalling. The mechanism(s) by which membrane @entity1420 receptor-a ( @entity27350 ) number is regulated on the cell surface is unclear. In this study, we investigated the cellular distribution of @entity27350 and mechanisms of @entity27350 trafficking using a cell line derived from a primary culture of @entity1 myometrial cells (M11) as an experimental model. RT-PCR and immunofluorescent analysis demonstrated expression of @entity27350 in M11 cells with @entity27350 primarily distributed on the cell surface under basal conditions. For the first time, plasma membrane localisation of @entity27350 was confirmed using immuno-gold transmission electron microscopy. Stimulation of M11 cells with @entity1420 (P4, 100 nM) resulted in internalisation of @entity27350 from the plasma membrane to the cytoplasm (10 min) and subsequent partial translocation back to the cell surface (20 min). We investigated potential endocytotic pathways involved in trafficking of @entity27350 after its internalisation. Partial co-localisation of clathrin with @entity27350 was obvious after 10 min of P4 treatment. Of note, @entity2644 (inhibitor of clathrin-mediated pathway) inhibited the endocytosis of @entity27350 , whereas treatment with @entity8701 (inhibitor of caveolae-mediated pathway) did not affect internalisation. Collectively, these data suggest that @entity27350 is expressed on the cell surface of M11 cells and undergoes endocytosis after P4 stimulation primarily via a clathrin-mediated pathway.

Internalisation of membrane XXXX receptor-a after treatment with @entity1420 : Potential involvement of a clathrin-dependent pathway.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1420 :: ('MESH:D011374', 'Chemical') :: ['progesterone'] @entity27350 :: ('20200(Tax:10090)', 'Gene') :: ['mPRa'] @entity2644 :: ('MESH:D002746', 'Chemical') :: ['chlorpromazine'] @entity8701 :: ('MESH:D009761', 'Chemical') :: ['nystatin']
@entity1420 :: (MESH:D011374,Chemical) :: ['progesterone']
