We developed a new neural transplantation protocol for the investigation of the repair of @entity131 . @entity75 was induced by touching a cold (-60 degrees C) metal stamp to the dura over the forelimb motor cortex of adult @entity35 . The procedure caused a localized lesion and the animals developed a significant @entity51 , which was monitored throughout the protocol. Six days later the animals received embryonic neural stem cells in the penumbra of the lesion. The donor cells were freshly isolated from E14 @entity35 embryos, had a high viability, and expressed the stem cell marker nestin. A further 6 days later the survival and differentiation of the grafted cells were investigated by immunohistochemistry. The majority of the surviving grafted cells were found in the lesion and they did not express lineage-specific markers. Only 10% of all surviving transplanted cells were located in the penumbra. These cells had an astrocytic phenotype and expressed glial fibrillary acidic protein. A few cells expressed neural or oligodendrocytic markers. In conclusion, we established a novel neural transplantation protocol, which focuses on cortical @entity131 . The model is a combination of surgical, neurological and histological approaches, all adapted to each other to make a reliable and reproducible experimental model.

Neural stem cell transplantation in cold lesion: a novel approach for the investigation of XXXX and repair.

@entity75 :: ('MESH:D001927', 'Disease') :: ['Cortical lesion'] @entity51 :: ('MESH:D009461', 'Disease') :: ['motor deficit'] @entity131 :: ('MESH:D001930', 'Disease') :: ['brain trauma'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats']
@entity131 :: (MESH:D001930,Disease) :: ['brain trauma']
