The real-time iontophoretic method using @entity30715 -selective microelectrodes and diffusion-weighted magnetic resonance imaging were used to measure the extracellular space volume fraction alpha, tortuosity lambda and apparent diffusion coefficient of water (ADC(W)) 240 min after the administration of @entity2134 in @entity4774 -anaesthetized @entity35 . The obtained data were correlated with extracellular @entity880 , @entity413 , and @entity2227 concentrations and the @entity880 / @entity2388 -ratio, determined by intracerebral microdialysis. The control values of alpha and lambda were 0.19+/-0.004 and 1.58+/-0.01, respectively. Following @entity2134 application, alpha decreased to 0.134+/-0.012 100 min later. Thereafter alpha increased, reaching 0.176+/-0.009 140 min later. No significant changes in lambda were observed during the entire time course of the experiment. ADC(W) was significantly decreased 100 min after @entity2134 application (549+/-8 microm(2) s(-1)) compared to controls (603+/-11 microm(2) s(-1)); by the end of the experiments, ADC(W) had returned to control values. The basal cortical levels of @entity880 , the @entity880 / @entity2388 ratio, @entity413 and @entity2227 were 0.61+/-0.05 mmol/l, 33.16+/-4.26, 2.42+/-0.13 mmol/l and 6.55+/-1.31 micromol/l. @entity2134 application led to a rise in @entity880 , the @entity880 / @entity2388 ratio and @entity2227 levels, reaching 2.92+/-0.60 mmol/l, 84.80+/-11.72 and 22.39+/-5.85 micromol/l within about 100 min, with a subsequent decrease to control values 140 min later. The time course of changes in @entity413 levels was different, with maximal levels of 3.49+/-0.24 mmol/l reached 40 min after @entity2134 injection and a subsequent decrease to 1.25+/-0.40 mmol/l observed 200 min later. Pathologically increased neuronal activity induced by @entity2134 causes cell swelling followed by a reduction in the ECS volume fraction, which can contribute to the accumulation of toxic metabolites and lead to the start of @entity302 discharges.

Brain metabolism and diffusion in the @entity35 cerebral cortex during XXXX -induced status epilepticus.

@entity2227 :: ('CHEBI:29987', 'Chemical') :: ['glutamate'] @entity4774 :: ('MESH:D014520', 'Chemical') :: ['urethane'] @entity2134 :: ('MESH:D010862', 'Chemical') :: ['Pilocarpine', 'pilocarpine'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity302 :: ('MESH:D004827', 'Disease') :: ['epileptic'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity30715 :: ('MESH:C027917', 'Chemical') :: ['tetramethylammonium'] @entity2388 :: ('MESH:D019289', 'Chemical') :: ['pyruvate']
@entity2134 :: (MESH:D010862,Chemical) :: ['pilocarpine']
