OBJECTIVE: To investigate the inhibitory effect of 6A8 alpha-manosidase expression on the adhesiveness of CNE-2L2 cells to laminin and the lamellipodia on cell surface. METHODS: 6A8 alpha-manosidase expression was detected by Western blotting. For assaying the adhesion of cells to laminin, cells were incubated in laminin-coated plate at 37 degrees C for 1 h, the adhered cells were stained with crystal purple dissolved in 0.1 mol/L @entity4958 /50% @entity1068 . Absorbance 540 nm was measured. Adhesion rate (R) was calculated according to formula R = AT/A100 x 100%. Here A100 represents 100% adhesion. lamellipodia on cell surface was observed upon a scanning electron microscopy. RESULTS: The adhesion rate of two clones ( @entity1673 and AS2) with inhibition of 6A8 alpha-manosidase expression to laminin was 0.447 +/- 0.096 and 0.533 +/- 0.065 respectively. The adhesion rate of three controls with normal expression of 6A8 alpha-manosidase to laminin was 0.78 +/- 0.035, 0.7 +/- 0.05 and 0.80 +/- 0.04 respectively. The difference was significant (P < 0.01). CNE-2L2 cells with normal expression of 6A8 alpha-manosidase was rich in lamellipodia on their surface. Lamellipodia nearly disappeared on the cells with inhibition of 6A8 alpha-manosidase expression. CONCLUSIONS: Inhibition of 6A8 alpha-manosidase expression results in decrease of adhesion to laminin and reduction of lamellipodia of @entity1 @entity189 cell CNE-2L2.

[Inhibition of 6A8 alpha-manosidase expression induces decrease of adhesion to laminin and reduction of lamellipodia of @entity1 XXXX cell CNE-2L2].

@entity1 :: ('9606', 'Species') :: ['human'] @entity4958 :: ('MESH:C102006', 'Chemical') :: ['Sodium Citrate'] @entity189 :: ('MESH:D002277', 'Disease') :: ['nasopharyngeal carcinoma'] @entity1673 :: ('5729', 'Gene') :: ['AS1'] @entity1068 :: ('MESH:D000431', 'Chemical') :: ['ethanol']
@entity189 :: (MESH:D002277,Disease) :: ['nasopharyngeal carcinoma']
