UNASSIGNED: Fermented @entity9688 spoilage (FCS) characterized by rising pH and the appearance of manure- and cheese-like aromas is a challenge of significant economical impact for the pickling industry. Previous culture-based studies identified the @entity375 Pichia manshurica and Issatchenkia occidentalis, 4 Gram-positive bacteria, @entity30050 , @entity30050 parrafaraginis, Clostridium sp., and Propionibacterium and 1 Gram-negative genus, Pectinatus, as relevant in various stages of FCS given their ability to metabolize @entity880 . It was the objective of this study to augment the current knowledge of FCS using culture-independent methods to microbiologically characterize commercial spoilage samples. Ion Torrent data and 16S rRNA cloning library analyses of samples collected from commercial fermentation tanks confirmed the presence of L. rapi and @entity30050 and revealed the presence of additional species involved in the development of FCS such as @entity30050 namurensis, @entity30050 acetotolerans, @entity30050 panis, @entity37106 peroxydans, @entity33182 , and @entity37106 at pH below 3.4. The culture-independent analyses also revealed the presence of species of Veillonella and Dialister in spoilage samples with pH above 4.0 and confirmed the presence of Pectinatus spp. during @entity880 degradation at the higher pH. @entity37106 spp. were successfully isolated from commercial samples collected from tanks subjected to air purging by plating on @entity2656 @entity375 Peptone agar. In contrast, @entity30050 spp. were primarily identified in samples of FCS collected from tanks not subjected to air purging for more than 4 mo. Thus, it is speculated that @entity26 availability may be a determining factor in the initiation of spoilage and the leading microbiota.

Bacterial Ecology of Fermented XXXX Rising pH Spoilage as Determined by Nonculture-Based Methods.

@entity2656 :: ('MESH:D008353', 'Chemical') :: ['Mannitol'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity30050 :: ('1581', 'Species') :: ['L. buchneri', 'Lactobacillus buchneri', 'Lactobacillus'] @entity9688 :: ('3659', 'Species') :: ['cucumber'] @entity33182 :: ('435', 'Species') :: ['Acetobacter aceti'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactic acid'] @entity375 :: ('4932', 'Species') :: ['yeasts', 'Yeast'] @entity37106 :: ('438', 'Species') :: ['Acetobacter', 'Acetobacter pasteurianus']
@entity9688 :: (3659,Species) :: ['Cucumber']
