AIM: To investigate the effect of @entity167 and/or @entity4047 on the expression of @entity13239 chain gene ( @entity13239 , @entity1414 ) mRNA and collagen production of @entity1 lung fibroblast( @entity8173 ). METHODS: @entity8173 was cultured and real time RT-PCR was used to determine the expression of @entity4047 receptor ( @entity16210 , @entity9596 , @entity9597 ) mRNA, @entity13239 mRNA and @entity1414 mRNA. ELISA was used to determine the production of collagen type I. RESULTS: Low level of @entity167 (25, 50, 100, 200 mmol/L) greatly up-regulated the expression of @entity16210 mRNA and @entity9597 mRNA of @entity8173 , but @entity9596 mRNA expression was decreased. low level of @entity167 (25, 50, 100, 200 mmol/L) had no effect on the expression of @entity13239 and @entity1414 . @entity4047 (10, 20, 50  g/L) stimulated the expression of @entity13239 and @entity1414 in a dose-dependent manner. The expression of @entity13239 and @entity1414 induced by combination of @entity167 (200 mmol/L) and @entity4047 (10  g/L, 20  g/L, 50  g/L) was significantly higher than @entity4047 , 50  g/L) alone. Combination of @entity167 (200 mmol/L) and @entity23226 ) could significantly increase collagen typeI production than @entity4047 , 50  g/L) alone. CONCLUSION: Low level of @entity167 has no effect on the mRNA expression of @entity13239 and @entity1414 of HFL-1cells, but has great effect on the mRNA expression of @entity16210 , @entity9596 and @entity9597 . Combination of @entity167 and @entity4047 significantly up-regulated the collagen production and expression of @entity13239 and @entity1414 of @entity8173 cells.

[Effects of XXXX and @entity167 on collagen expression of @entity1 lung fibrolasts].

@entity1 :: ('9606', 'Species') :: ['human'] @entity1414 :: ('1281', 'Gene') :: ['COL3A1'] @entity8173 :: ('3078', 'Gene') :: ['HFL-1'] @entity9597 :: ('3566', 'Gene') :: ['IL-4Ra'] @entity4047 :: ('3596', 'Gene') :: ['IL-13', 'IL-13(10, 20'] @entity23226 :: ('50604', 'Gene') :: ['IL-13(10, 20, 50  g/L'] @entity167 :: ('CHEBI:16236', 'Chemical') :: ['alcohol', 'Alcohol'] @entity9596 :: ('3598', 'Gene') :: ['IL-13Ra2'] @entity13239 :: ('1277', 'Gene') :: ['COL1A1', 'collagen type I alpha 1'] @entity16210 :: ('3597', 'Gene') :: ['IL-13Ra1']
@entity4047 :: (3596,Gene) :: ['IL-13']
