Several pesticides suspected or known to have endocrine disrupting effects were screened for pro- or antiandrogenic properties by determining their effects on proliferation, prostatic-specific antigen (PSA) secretion and @entity342 receptor (AR) expression, and AR phosphorylation in @entity342 -dependent LNCaP @entity1 @entity263 cells, as well as on the expression and catalytic activity of the enzyme @entity9667 in H295R @entity1 @entity1636 cells, an in  vitro model of steroidogenesis. Effects on SRD5A gene expression were determined in both cell lines. @entity13642 , @entity30173 , and @entity22432 , but not @entity12472 , concentration dependently (1-30    M) decreased @entity586 ( @entity587 )-stimulated proliferation of LNCaP cells. All pesticides except @entity12472 decreased @entity587 -stimulated PSA secretion, AR nuclear accumulation, and AR phosphorylation on serines 81 and 213 in LNCaP cells. @entity13642 and @entity22432 , but not @entity30173 or @entity12472 , decreased levels of @entity9667 gene and protein expression, as well as catalytic activity in H295R cells. In the case of @entity22432 , some of these effects corresponded with @entity137 . H295R cells expressed AR protein and @entity10831 , but not @entity35006 transcripts. @entity10831 gene expression in H295R cells was increased by 10   nM @entity587 , whereas in LNCaP cells significant induction was observed by 0.1   nM @entity587 . AR protein expression in H295R cells was not increased by @entity587 . @entity30173 decreased @entity587 -induced @entity10831 gene expression in LNCaP, but not H295R cells, indicating a functional difference of AR between the cell lines. In conclusion, pesticides may exert antiandrogenic effects through several mechanisms that are cell type-specific, including AR antagonism and down-regulation or catalytic inhibition of @entity342 biosynthetic enzymes, such as @entity9667 and @entity10831 .

Antiandrogenic Mechanisms of Pesticides in XXXX LNCaP Prostate and H295R @entity1636 Cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity35006 :: ('6716', 'Gene') :: ['SRD5A2'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity263 :: ('MESH:D011471', 'Disease') :: ['prostate cancer'] @entity586 :: ('MESH:D013196', 'Chemical') :: ['dihydrotestosterone'] @entity9667 :: ('1586', 'Gene') :: ['CYP17'] @entity587 :: ('CHEBI:16330', 'Chemical') :: ['DHT'] @entity22432 :: ('MESH:C045362', 'Chemical') :: ['prochloraz'] @entity12472 :: ('MESH:D001280', 'Chemical') :: ['atrazine'] @entity10831 :: ('6715', 'Gene') :: ['SRD5A1'] @entity1636 :: ('MESH:D018268', 'Disease') :: ['adrenocortical carcinoma'] @entity342 :: ('CHEBI:50113', 'Chemical') :: ['androgen'] @entity30173 :: ('MESH:C025643', 'Chemical') :: ['Vinclozolin', 'vinclozolin'] @entity13642 :: ('MESH:D001542', 'Chemical') :: ['Benomyl']
@entity1 :: (9606,Species) :: ['Human']
