UNASSIGNED: @entity1014 kinases (TKs) are important components in the @entity4334 salvage pathway. However, knowledge about plant TKs is quite limited. In this study, the molecular function of TKs in @entity958 was investigated. Two TKs were identified and named AtTK1 and AtTK2. Expression of both genes was ubiquitous, but AtTK1 was strongly expressed in high-proliferation tissues. AtTK1 was localized to the cytosol, whereas AtTK2 was localized to the mitochondria. Mutant analysis indicated that the two genes function coordinately to sustain normal plant development. Enzymatic assays showed that the two TK proteins shared similar catalytic specificity for @entity7392 . They were able to complement an @entity526 lacking TK activity. @entity10049 ( @entity33243 ) resistance and @entity10080 ( @entity10081 ) incorporation assays confirmed their activity in  vivo. Furthermore, the tk mutant phenotype could be alleviated by @entity4334 feeding, establishing that the biosynthesis of @entity17450 was disrupted by the @entity942 . Finally, both @entity1 and @entity579 ( @entity579 ) TKs were able to rescue the tk mutants, demonstrating the functional conservation of TKs across organisms. Taken together, our findings clarify the specialized function of two TKs in A. thaliana and establish that the salvage pathway mediated by the kinases is essential for plant growth and development.

XXXX kinases share a conserved function for @entity4334 salvage and play an essential role in @entity958 growth and development.

@entity1 :: ('9606', 'Species') :: ['human'] @entity10081 :: ('MESH:C032766', 'Chemical') :: ['EdU'] @entity17450 :: ('MESH:D011742', 'Chemical') :: ['pyrimidine nucleotides'] @entity958 :: ('3702', 'Species') :: ['Arabidopsis thaliana'] @entity10080 :: ('MESH:C031086', 'Chemical') :: ["5-ethynyl 2'-deoxyuridine"] @entity10049 :: ('MESH:D005467', 'Chemical') :: ["5'-Fluorodeoxyuridine"] @entity579 :: ('4530', 'Species') :: ['Oryza sativa', 'rice'] @entity1014 :: ('MESH:D013936', 'Chemical') :: ['Thymidine'] @entity33243 :: ('CHEBI:60761', 'Chemical') :: ['FdU'] @entity4334 :: ('D009711', 'Chemical') :: ['nucleotide'] @entity942 :: ('MESH:D003677', 'Disease') :: ['TK deficiency'] @entity7392 :: ('MESH:D011741', 'Chemical') :: ['pyrimidine nucleosides'] @entity526 :: ('562', 'Species') :: ['Escherichia coli strain']
@entity1014 :: (MESH:D013936,Chemical) :: ['Thymidine']
