OBJECTIVE: The aim of this study was to investigate the effect of polypeptide from @entity19852 (PCF) on ultraviolet B (UVB) irradiation-induced apoptosis in @entity1 keratinocyte HaCaT cells. METHODS: HaCaT cells were treated with 20mJ/cm(2) UVB irradiation for 18h. The cell viability was measured by @entity98 assay, and apoptosis was detected with @entity2455 staining and caspase-3 activity detection. Protein expression levels were assessed by Western blot analysis, and the intracellular ROS levels were also measured. RESULTS: Our results from the @entity98 assay showed that UVB irradiation significantly declined the viability of HaCaT cells, which could be restored by PCF treatment. PCF decreased the apoptosis rate in HaCaT cells treated with UVB irradiation. Moreover, PCF increased the expression levels of PDI and Ero-1a, and scavenged the intracellular ROS. Furthermore, PCF inhibited the expressions of GRP78, p-PERK, p-eIF2a, and CHOP, and suppressed the ER stress-induced apoptosis, in UVB-irradiated HaCaT cells. In addition, the ROS scavenging effect of @entity10023 was less potent than PCF, indicating that ER stress-related ROS production contribute partially to the total ROS level, and ER was not the only target of PCF treatment. CONCLUSIONS: Our results indicate that PCF inhibits UVB irradiation-induced apoptosis through restoring ER redox homeostasis and suppressing the PERK-eIF2a-CHOP pathway. These findings provide evidence for the application of PCF in the protection of skin from UV irradiation.

Polypeptide from XXXX suppresses ultraviolet-B irradiation-induced apoptosis through restoring ER redox homeostasis, scavenging ROS generation, and suppressing the PERK-eIF2a-CHOP pathway in HaCaT cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity2455 :: ('MESH:D006690', 'Chemical') :: ['Hoechst 33258'] @entity19852 :: ('202578', 'Species') :: ['Chlamys farreri'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity10023 :: ('MESH:C121358', 'Chemical') :: ['4-PBA']
@entity19852 :: (202578,Species) :: ['Chlamys farreri']
