We had previously found that a @entity9901 -soluble protein ( @entity55384 ) occurs in @entity35 liver, and that this novel protein inhibits protein synthesis in a @entity95 reticulocyte lysate system (T. Oka, H. Tsuji, C. Noda, K. Sakai, Y.-H. Hong, I. @entity6953 , S. Mu  oz, Y. Natori, J. Biol. Chem. 270 (1995) 30060-30067). In the present study, we analyzed lipid components bound to @entity55384 . Native @entity55384 was purified from @entity35 liver using Sephadex G-75, DE-52 cellulose and IgGPSP-affinity chromatography, and the lipid components were extracted. The components obtained from the purified @entity55384 were shown to be free @entity1718 by thin-layer chromatography. By GC-MS, six major @entity1718 were identified as 14:0, 16:0, 18:0, 18:1, 18:2 and 20:4. 1 mol of @entity55384 contained 1.26 mol of total @entity844 components. The @entity844 -binding assay of @entity55384 showed that the Bmax was 1.25 mol @entity844 /mol @entity55384 and the Kd value for @entity7609 was 6.03 microM. The concentration of @entity55384 mRNA in @entity35 liver increased 2.3-fold by the administration of peroxisome proliferator, @entity10569 . These findings show that @entity55384 is a @entity844 -binding protein-like protein, which is involved in the intracellular metabolism of @entity844 and is quite different from the known @entity844 -binding proteins.

Analysis of the XXXX components in a @entity9901 -soluble protein.

@entity55384 :: ('65151(Tax:10116)', 'Gene') :: ['PSP1'] @entity844 :: ('CHEBI:35366', 'Chemical') :: ['fatty acid'] @entity10569 :: ('MESH:D001629', 'Chemical') :: ['bezafibrate'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity9901 :: ('MESH:D010472', 'Chemical') :: ['perchloric acid'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity1718 :: ('MESH:D005227', 'Chemical') :: ['fatty acids'] @entity7609 :: ('MESH:D019308', 'Chemical') :: ['palmitic acid'] @entity6953 :: ('8164', 'Species') :: ['Suzuki']
@entity844 :: (CHEBI:35366,Chemical) :: ['fatty acid']
