UNASSIGNED: Transarterial chemoembolization (TACE) is used to treat various types of @entity5 such as @entity157 and @entity703 . However, embolization and blocking of blood vessels nourishing a @entity5 mass evokes an angiogenic response due to the secretion of @entity7 ( @entity7 ), which results in the formation of new blood vessels and eventually limitation in therapeutic efficacy. The presented work investigates the feasibility of loading the clinically used embolic beads (DC Bead  ) with Bevacizumab (BEV), an anti- @entity7 antibody, and control its release kinetics via Layer-by-Layer (LbL) coating. This strategy has the aim to achieve high, localized and sustained concentrations of @entity1493 at the @entity5 site and reduce drug exposure in the systemic circulation. High loading of @entity1493 on lyophilized beads of about 76mg @entity1493 /bead vial was achieved. LbL coating was carried out by depositing alternating layers of the biocompatible polymers alginate and @entity5731 . Coating was proven successful by monitoring the reversal of zeta potential after addition of each layer. Morphological changes of the bead surface before and after coating were illustrated using SEM imaging. Moreover, release profiles from different formulations were studied and results showed that optimizing the number of deposited layers effectively slows the release of @entity1493 for three days. Activity of released @entity1493 was studied in different 2D and 3D cell based assays. Released @entity1493 fractions showed comparable activity to fresh @entity1493 solution used as control after 3days. In conclusion, our results suggest the opportunity for loading anti- @entity7 antibodies on commercially available embolic beads to increase the efficacy of TACE of @entity5 .

Arming embolic beads with anti- XXXX antibodies and controlling their release using LbL technology.

@entity5731 :: ('CHEBI:61490', 'Chemical') :: ['poly-L-lysine'] @entity7 :: ('7422', 'Gene') :: ['vascular endothelial growth factor', 'VEGF'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'hypervascular tumors'] @entity1493 :: ('12064', 'Species') :: ['BEV'] @entity157 :: ('MESH:D006528', 'Disease') :: ['hepatocellular carcinoma'] @entity703 :: ('MESH:D007680', 'Disease') :: ['renal cancer']
@entity7 :: (7422,Gene) :: ['VEGF']
