A grande @entity66625 disruptant mutant of @entity375 was generated by crossing a petite disruptant to a wild-type grande strain. This strain was relatively stable, but generated petites at an elevated frequency, illustrating the ancillary role of @entity2041 ( @entity2197 ) in the maintenance of the genetic integrity of the mitochondrial genome. The availability of the grande @entity66625 deletant enabled an evaluation of the role of @entity2197 in the cellular response to @entity273 independent of the effects of a petite mutation. The mutant strain was more sensitive to @entity273 than the wild-type strain but was still capable of producing an adaptive stress response to this compound. @entity2197 was found to be essential for growth and sporulation of the @entity375 , but the intracellular level needed to support growth was at least two orders of magnitude less than that normally present in wild-type cells. This surprising result indicates that there is an essential role for @entity2197 but only very low amounts are needed for growth. This result was also found in anaerobic conditions, thus this essential function does not involve protection from oxidative stress. Suppressors of the @entity66625 deletion mutation were isolated by @entity6321 mutagenesis. These were the result of a single recessive mutation (sgr1, suppressor for @entity2041 requirement) that relieved the requirement for @entity2197 for growth on minimal medium but did not affect the sensitivity to @entity274 stress. Interestingly, the @entity66625 sgr1 mutant generated petites at a lower rate than the @entity66625 mutant. Thus, it is suggested that the essential role of @entity2197 is involved in the maintenance of the mitochondrial genome.

The essential and ancillary role of @entity2041 in @entity375 analysed using a grande XXXX disruptant strain.

@entity6321 :: ('MESH:D005020', 'Chemical') :: ['ethylmethanesulfonate'] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H(2)O(2)'] @entity2041 :: ('MESH:D005978', 'Chemical') :: ['glutathione'] @entity2197 :: ('CHEBI:16856', 'Chemical') :: ['GSH'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['hydrogen peroxide'] @entity66625 :: ('853344(Tax:4932)', 'Gene') :: ['gsh1'] @entity375 :: ('4932', 'Species') :: ['yeast', 'Saccharomyces cerevisiae']
@entity66625 :: (853344(Tax:4932),Gene) :: ['gsh1']
