@entity293 are the most common @entity5450 in the United States and one of the most common @entity2348 of any organ system. Biofilms persist in the urinary tract and on catheter surfaces because biofilm microorganisms are resistant to host defense mechanisms and antibiotic therapy. The first step in the establishment of biofilm infections is @entity2348 ; preventing @entity2348 represents a promising method of controlling biofilms. Evidence suggests that capsular polysaccharides play a role in adhesion and pathogenicity. This study focuses on the role of physiochemical and specific binding interactions during adhesion of colanic acid exopolysaccharide mutant strains. Bacterial adhesion was evaluated for isogenic uropathogenic @entity526 strains that differed in colanic acid expression. The atomic force microscope (AFM) was used to directly measure the reversible physiochemical and specific binding interactions between bacterial strains and various substrates as bacteria initially approach the interface. AFM results indicate that electrostatic interactions were not solely responsible for the repulsive forces between the colanic acid mutant strains and hydrophilic substrates. Moreover, hydrophobic interactions were not found to play a significant role in adhesion of the colanic acid mutant strains. Adhesion was also evaluated by parallel-plate flow cell studies in comparison to AFM force measurements to demonstrate that prolonged incubation times alter @entity2348 . Results from this study demonstrate that the capsular polysaccharide @entity35716 does not enhance @entity2348 but rather blocks the establishment of specific binding as well as time-dependent interactions between uropathogenic @entity526 and inert substrates.

Role of capsular colanic acid in adhesion of uropathogenic XXXX .

@entity2348 :: ('MESH:D001424', 'Disease') :: ['bacterial infections', 'bacterial adhesion'] @entity5450 :: ('MESH:D014570', 'Disease') :: ['urologic disease'] @entity35716 :: ('MESH:C004275', 'Chemical') :: ['colanic acid'] @entity293 :: ('MESH:D014552', 'Disease') :: ['Urinary tract infections'] @entity526 :: ('562', 'Species') :: ['Escherichia coli', 'E. coli']
@entity526 :: (562,Species) :: ['Escherichia coli']
