1. A study of wall synthesis has been made by following the incorporation of radioactive @entity413 and @entity1889 into the cytoplasm and wall of @entity375 . 2. Both @entity413 and @entity1889 are incorporated into a mannan glycopeptide. The @entity413 is also synthesized into a structural glucan of the wall. 3. The mannan glycopeptide contains high-molecular-weight mannan and low-molecular-weight @entity3913 and oligosaccharide units composed of @entity3913 . Both types of @entity1735 are attached to the peptide. The extent of radioactive incorporation into these different @entity1735 constituents of the glycopeptide remained constant during a pulse-chase experiment. No evidence of a sequential synthesis of oligosaccharides and high-molecular-weight mannan was obtained. 4. @entity1880 inhibits the incorporation of @entity1889 into the wall but only partially inhibits the incorporation of @entity413 . Thus not all the polysaccharide deposited into the wall is dependent on a simultaneous peptide synthesis and incorporation. 5. Protoplasts grown in an iso-osmotic medium secreted a mannan polymer that was probably a glycopeptide.

XXXX cell-wall synthesis.

@entity375 :: ('4932', 'Species') :: ['yeast'] @entity1880 :: ('MESH:D003513', 'Chemical') :: ['Cycloheximide'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['carbohydrate'] @entity3913 :: ('MESH:D008358', 'Chemical') :: ['mannose'] @entity1889 :: ('CHEBI:26986', 'Chemical') :: ['threonine'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity375 :: (4932,Species) :: ['Yeast']
