OBJECTIVE: To use in vitro assays to evaluate the effects of a novel immunosuppressive agent, @entity20083 , on biological functions (migration, phagocytosis, and production of reactive- @entity26 species [ROS]) of feline peripheral neutrophils and determine the cytotoxic effects of @entity20083 on feline peripheral neutrophils. SAMPLE POPULATION: Peripheral neutrophils obtained from 8 healthy @entity468 . PROCEDURE: Peripheral neutrophils were isolated from blood samples obtained from the 8 @entity468 and exposed to the phosphorylated form of @entity20083 ( @entity36920 ). A fluorescence-based in vitro evaluation of migration was performed. Phagocytosis of microbes and production of ROS were evaluated by use of a 2-color flow cytometry system. Samples of whole blood obtained from the @entity468 were incubated with various concentrations of @entity36920 , @entity941 -labeled @entity794 , and @entity16203 . Cytotoxic effects were evaluated by use of @entity2667 staining. RESULTS: Addition of @entity36920 caused a slight non-significant decrease in phagocytosis and production of ROS by feline peripheral neutrophils. Migration activity of feline peripheral neutrophils was significantly increased by the addition of @entity36920 . Addition of @entity36920 at concentrations considered for clinical use did not increase the death rate of feline peripheral neutrophils. CONCLUSIONS AND CLINICAL RELEVANCE: @entity20083 does not inhibit critical functions of feline peripheral neutrophils in vitro.

In vitro evaluation of the effect of a novel immunosuppressive agent, XXXX , on the function of feline neutrophils.

@entity2667 :: ('MESH:D011419', 'Chemical') :: ['propidium iodide'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity20083 :: ('MESH:C098720', 'Chemical') :: ['FTY720'] @entity794 :: ('1280', 'Species') :: ['Staphylococcus aureus'] @entity36920 :: ('MESH:C525008', 'Chemical') :: ['FTY720-P'] @entity941 :: ('MESH:D019793', 'Chemical') :: ['fluorescein'] @entity468 :: ('9685', 'Species') :: ['cats'] @entity16203 :: ('MESH:C067883', 'Chemical') :: ['dihydroethidium']
@entity20083 :: (MESH:C098720,Chemical) :: ['FTY720']
