Previously, we reported that intravenous infusion of apoptotic leukocytes facilitated bone marrow (BM) engraftment across major histocompatibility barriers. This cell-based therapeutic approach is of great interest for stem cell therapy across histocompatibility barriers. Autoimmunity associated with apoptotic cell administration may, however, limit the use of this approach. Indeed, autoantigens are concentrated on the surface of apoptotic cells, and defective clearance of apoptotic bodies is associated with the occurrence of @entity792 . In consequence, we assessed the autoimmune responses raised against ubiquitous double-stranded DNA (dsDNA) and cardiolipin autoantigens following a single intravenous infusion of apoptotic cells simultaneously to allogeneic BM administration. No difference was observed between levels of natural circulating immunoglobulin M (IgM) (anti-dsDNA and anti- @entity6894 ) autoantibodies found in @entity19 receiving allogeneic BM alone and those found in @entity19 receiving apoptotic cells also. Pathogenic IgG autoantibody titers after apoptotic cell infusion were 9- to 200-fold lower than autoantibody titers found in lupus-prone @entity19 and not different from titers detected in BM grafted @entity19 . Kinetic analysis of autoantibodies after transplantation did not demonstrate any immunization against tested autoantigens after apoptotic cell infusion. Finally, neither immune complex deposition nor specific lesions were observed in the renal glomeruli of @entity19 infused with apoptotic cells 9 months post-BM transplantation. Overall, these results show no specific @entity137 of a single infusion of apoptotic cells administrated simultaneously to BM and may also shed light on factors influencing the immunogenic properties of apoptotic cells.

A single intravenous infusion of apoptotic cells, an alternative cell-based therapy approach facilitating hematopoietic cell engraftment, did not induce XXXX .

@entity19 :: ('10090', 'Species') :: ['mice'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity792 :: ('MESH:D001327', 'Disease') :: ['systemic autoimmune disease'] @entity6894 :: ('MESH:D002308', 'Chemical') :: ['cardiolipin']
@entity792 :: (MESH:D001327,Disease) :: ['autoimmunity']
