This study assessed the importance of @entity701 in mediating inhibition of pulsatile LH secretion in @entity742 exposed to a psychosocial stress. First, we developed an acute psychosocial stress model that involves sequential layering of novel stressors over 3-4 h. This layered-stress paradigm robustly activated the @entity2339 and unambiguously inhibited pulsatile LH secretion. We next used this paradigm to test the hypothesis that @entity701 , acting via the type II glucocorticoid receptor (GR), mediates stress-induced suppression of pulsatile LH secretion. Our approach was to determine whether an antagonist of the type II GR ( @entity3927 ) reverses inhibition of LH pulsatility in response to the layered stress. We used two animal models to assess different aspects of LH pulse regulation. With the first model (ovariectomized ewe), LH pulse characteristics could vary as a function of both altered @entity1176 pulses and pituitary responsiveness to @entity1176 . In this case, antagonism of the type II GR did not prevent stress-induced inhibition of pulsatile LH secretion. With the second model (pituitary-clamped ovariectomized ewe), pulsatile @entity1176 input to the pituitary was fixed to enable assessment of stress effects specifically at the pituitary level. In this case, the layered stress inhibited pituitary responsiveness to @entity1176 and antagonism of the type II GR reversed the effect. Collectively, these findings indicate acute psychosocial stress inhibits pulsatile LH secretion, at least in part, by reducing pituitary responsiveness to @entity1176 . @entity701 , acting via the type II GR, is an obligatory mediator of this effect. However, under conditions in which @entity1176 input to the pituitary is not clamped, antagonism of the type II GR does not prevent stress-induced inhibition of LH pulsatility, implicating an additional pathway of suppression that is independent of @entity701 acting via this receptor.

Does XXXX acting via the type II glucocorticoid receptor mediate suppression of pulsatile luteinizing hormone secretion in response to psychosocial stress?

@entity742 :: ('9940', 'Species') :: ['sheep'] @entity2339 :: ('MESH:D007029', 'Disease') :: ['hypothalamic-pituitary-adrenal axis'] @entity701 :: ('MESH:D006854', 'Chemical') :: ['Cortisol', 'cortisol'] @entity1176 :: ('MESH:D007987', 'Chemical') :: ['GnRH'] @entity3927 :: ('MESH:D015735', 'Chemical') :: ['RU486']
@entity701 :: (MESH:D006854,Chemical) :: ['cortisol']
