BACKGROUND: Previous study showed that extracts from Croton macrostachyus (Euphorbiaceae) exhibit analgesic effects in @entity12761 models. The present study evaluates the antinociceptive properties of the @entity1042 / @entity1044 extract (MECM) of the stem bark of this plant using @entity19 models of persistent inflammatory and @entity9 , and assesses its mechanism of action. METHODS: MECM was tested on Complete Freund adjuvant ( @entity3795 )-induced persistent thermal and mechanical @entity158 , @entity9 induced by partial sciatic nerve ligation (PSNL), @entity1421 ( @entity1421 )-induced acute @entity1931 , as well as on nociception induced by @entity2951 in @entity19 . Mechanical @entity1931 was assessed using von Frey hair in awake @entity19 . The mechanism of action of MECM was evaluated by using @entity5217 on @entity1421 -induced @entity1931 or @entity351 on @entity2951 -induced @entity158 . RESULTS: MECM administered orally at the doses of 250 and 500  mg/kg, induced long lasting and significant antihyperalgesic effects on @entity3795 -inflammatory and PSNL-induced @entity9 . MECM significantly reduced the @entity1931 induced by @entity1421 either when administered preventively or therapeutically. @entity65720 also significantly and time dependently inhibited the @entity2951 -induced nociception. These effects were not affected by @entity5217 or by @entity351 . CONCLUSIONS: The present results demonstrate that the oral administration of MECM to @entity19 resulted in long lasting antihyperalgesic activity in inflammatory and @entity9 as well as in acute and persistent @entity158 . The mechanism underlying the long lasting MECM antihyperalgesic effect is currently unknown, but might be mediated, at least partially, through the modulation of @entity4412 receptors.

XXXX channel inhibition contributes to the antinociceptive effects of Croton macrostachyus extract in @entity19 .

@entity1931 :: ('MESH:D006930', 'Disease') :: ['mechanical hyperalgesia', 'hyperalgesia'] @entity1044 :: ('MESH:D008752', 'Chemical') :: ['methylene chloride'] @entity1042 :: ('MESH:D000432', 'Chemical') :: ['methanol'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity1421 :: ('MESH:D015232', 'Chemical') :: ['PGE2', 'prostaglandin E2'] @entity2951 :: ('MESH:D002211', 'Chemical') :: ['capsaicin'] @entity5217 :: ('MESH:D005905', 'Chemical') :: ['glibenclamide'] @entity65720 :: ('MESH:C064380', 'Chemical') :: ['MECM'] @entity4412 :: ('193034(Tax:10090)', 'Gene') :: ['TRPV1'] @entity3795 :: ('31658', 'Species') :: ['CFA'] @entity158 :: ('MESH:D010146', 'Disease') :: ['pain'] @entity12761 :: ('MESH:D059787', 'Disease') :: ['acute pain'] @entity9 :: ('MESH:D009437', 'Disease') :: ['neuropathic pain'] @entity351 :: ('MESH:C089032', 'Chemical') :: ['rimonabant']
@entity4412 :: (193034(Tax:10090),Gene) :: ['TRPV1']
