UNASSIGNED: Radiation treatment is a pivotal therapy for several @entity5 types, including @entity14 . It has been shown that sublethal doses of radiation modulate gene expression, making @entity5 cells more susceptible to T-cell-mediated immune attack. We have recently shown that low dose radiation enhances expression of multiple death receptors (Fas, @entity4107 and @entity3049 ) and co-stimulatory molecules ( @entity44085 and @entity16348 ) in @entity14 ( @entity14 ) cells; however, it is unclear how ionizing radiation (IR) enhances expression of these molecules mechanistically. In the present study, we elucidate the molecular mechanisms by which radiation controls expression of these molecules in @entity14 . Here we report that, enhanced expression of these genes following radiation treatment of @entity14 cells is due, in part, to changes in DNA methylation and histone acetylation. We observed that radiation (5  Gy) significantly increased histone acetylation at the promoter regions of @entity44085 , Fas and @entity3049 but not @entity16348 . However, radiation did not induce changes in the global levels of acetylated histone  H3 suggesting specificity of IR-induced changes. Furthermore, evaluation of epigenetic controlling enzymes revealed that IR did not alter overall cellular levels of HDACs ( @entity17029 , @entity15687 or @entity3997 ) or DNMTs ( @entity16610 , @entity15786 , or @entity15894 ). Instead, radiation decreased binding of @entity15687 and @entity3997 at the promoter regions of Fas and @entity44085 , respectively. Radiation also resulted in reduced @entity16610 at both the Fas and @entity44085 promoter regions but not a control gene. We conclude that single dose radiation can influence the expression of immune response relevant genes in @entity14 cells by altering the binding of epigenetic enzymes, and modulating histone acetylation, at specific gene promoters.

Radiation-induced modulation of immunogenic genes in XXXX cells is regulated by both histone deacetylases and DNA methyltransferases.

@entity15894 :: ('1789', 'Gene') :: ['DNMT3b'] @entity15687 :: ('3066', 'Gene') :: ['HDAC2'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity3997 :: ('8841', 'Gene') :: ['HDAC3'] @entity44085 :: ('8744', 'Gene') :: ['4-1BBL'] @entity3049 :: ('8795', 'Gene') :: ['DR5'] @entity16348 :: ('7292', 'Gene') :: ['OX-40L'] @entity14 :: ('MESH:D015179', 'Disease') :: ['CRC', 'colorectal tumor', 'colorectal cancer'] @entity17029 :: ('3065', 'Gene') :: ['HDAC1'] @entity15786 :: ('1788', 'Gene') :: ['DNMT3a'] @entity4107 :: ('3126', 'Gene') :: ['DR4'] @entity16610 :: ('1786', 'Gene') :: ['DNMT1']
@entity5 :: (MESH:D009369,Disease) :: ['tumor']
