UNASSIGNED: Elucidation of the process of degeneration of injured axons is important for the development of therapeutic modules for the treatment of @entity1566 . The aim of this study was to establish a method for time-lapse observation of injured axons in living animals after @entity1566 . YFP (yellow fluorescent protein)-H @entity19 , which we used in this study, express fluorescence in their nerve fibers. Contusion damage to the spinal cord at the 11th vertebra was performed by IH (Infinite Horizon) impactor, which applied a pressure of 50 kdyn. The damaged spinal cords were re-exposed during the observation period under anesthesia, and then observed by two-photon excited fluorescence microscopy, which can observe deep regions of tissues including spinal cord axons. No significant morphological change of injured axons was observed immediately after injury. Three days after injury, the number of axons decreased, and residual axons were fragmented. Seven days after injury, only fragments were present in the damaged tissue. No hind-limb movement was observed during the observation period after injury. Despite the immediate paresis of hind-limbs following the @entity130 , the morphological degeneration of injured axons was delayed. This method may help clarification of pathophysiology of @entity528 and development of therapeutic modules for the treatment of @entity1566 .

Evaluation of Injured Axons Using Two-Photon Excited Fluorescence Microscopy after Spinal Cord Contusion Injury in YFP-H Line XXXX .

@entity1566 :: ('MESH:D013119', 'Disease') :: ['spinal cord injuries', 'spinal cord injury', 'spinal cord contusion injury'] @entity19 :: ('10090', 'Species') :: ['transgenic mice'] @entity528 :: ('MESH:D009410', 'Disease') :: ['axon degeneration'] @entity130 :: ('MESH:D014947', 'Disease') :: ['contusion injury']
@entity19 :: (10090,Species) :: ['Mice']
