1. The hormonal regulation of phosphofructokinase was investigated in the accessory reproductive organs of the orchidectomized @entity35 . 2. Phosphofructokinase activities declined to 51% and 47% in the prostate and 9% and 6% of the normal values in seminal vesicles 4 and 8 weeks after castration respectively. Administration of @entity251 (100mug./100g. body wt.) for 3 days reversed substantially the effects of orchidectomy, and phosphofructokinase activity increased to 173% in the prostate and 536% in seminal vesicles as compared with the values of castrated controls. 3. Time-course studies demonstrated that after a single injection of @entity251 (5mg./100g. body wt.) phosphofructokinase activity was maximally elevated to 236% in the prostate and 342% in seminal vesicles at 24hr. 4. Dose-response studies revealed that 2.5mg. of @entity3033 /100g. body wt. was the minimal amount necessary to induce significant increases in enzyme activity in both accessory sex organs; maximal increases were obtained with a dose of 5mg./100g. body wt. 5. The observed enzyme increases induced by @entity251 were inhibited by the simultaneous administration of @entity53 -17beta, and phosphofructokinase activity in this group of @entity35 remained at 97% in the prostate and 137% of the control values in seminal vesicles. @entity53 -17beta by itself failed to produce any significant effect on enzyme activity in either of these secondary sexual tissues. 6. The nature of the @entity251 -induced increases in phosphofructokinase activity was studied by using a variety of inhibitors of RNA and protein synthesis. @entity1880 , @entity1487 and @entity10452 largely blocked the @entity342 -stimulated rise in enzyme activity observed 24hr. after @entity134 injection. The inhibitory effect of @entity10452 was completely reversed by the simultaneous administration of @entity1853 . 7. @entity4316 , which is known to inhibit the synthesis of messenger RNA as well as the synthesis of other cellular RNA fractions, when given simultaneously with the hormone, also inhibited the @entity251 -induced @entity1069 . However, when the antibiotic was given 6 or 12hr. after injection of the @entity134 , practically no inhibition of phosphofructokinase induction was obtained. This indicates that, once the enzyme-forming machinery is turned on and allowed to operate for a few hours, actinomycin is incapable of reversing the hormone-induced enzyme responses. 8. The results presented suggest that new RNA and protein synthesis may be involved in the observed @entity342 -induced increases in phosphofructokinase activity in the prostate and seminal vesicles of the orchidectomized @entity35 .

Metabolic control mechanisms in mammalian systems. Hormonal regulation of phosphofructokinase in the XXXX prostate and seminal vesicles.

@entity3033 :: ('MESH:D043343', 'Chemical') :: ['testosterone propionate'] @entity251 :: ('MESH:D013739', 'Chemical') :: ['testosterone'] @entity1069 :: ('MESH:D053448', 'Disease') :: ['increases in prostatic and seminal-vesicular phosphofructokinase'] @entity1880 :: ('MESH:D003513', 'Chemical') :: ['Cycloheximide'] @entity4316 :: ('MESH:D003609', 'Chemical') :: ['Actinomycin'] @entity53 :: ('MESH:D004958', 'Chemical') :: ['oestradiol', 'Oestradiol'] @entity1853 :: ('MESH:D008715', 'Chemical') :: ['methionine'] @entity134 :: ('CHEBI:35341', 'Chemical') :: ['steroid'] @entity10452 :: ('MESH:D005001', 'Chemical') :: ['ethionine'] @entity342 :: ('CHEBI:50113', 'Chemical') :: ['androgen'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity1487 :: ('MESH:D005472', 'Chemical') :: ['5-fluorouracil']
@entity35 :: (10116,Species) :: ['rat']
