UNASSIGNED: Controversy remains whether the leukocyte genomic response to @entity130 or @entity295 is dependent upon the initiating stimulus. Previous work illustrated poor correlations between historical models of @entity19 @entity130 and @entity295 (i.e. @entity548 and lipopolysaccharide injection, respectively). The aim of this study is to examine the early genomic response in improved @entity19 models of @entity295 (cecal ligation and puncture; @entity12147 ) and @entity130 ( @entity1230 ; PT) with and without @entity55 (PT+Pp). Groups of naive, @entity12147 , PT, and PT+Pp @entity19 were sacrificed at two hours, one or three days. Total leukocytes were isolated for genome-wide expression analysis and genes that were found to differ from control (FDR adjusted p<0.001) were assessed for fold-change differences. Spearman correlations were also performed. For all time points combined ( @entity12147 , PT, PT+Pp), there were 10,426 total genes that were found to significantly differ from naive controls. At two hours, the transcriptomic changes between @entity12147 and PT showed a positive correlation (rs) of 0.446 (p<0.0001), but were less positive thereafter. Correlations were significantly improved when limiting the analysis to common genes whose expression differed by a 1.5 fold-change. Both pathway and upstream analyses revealed the activation of genes known to be associated with PAMP and DAMP signaling, and early activation patterns of expression were very similar between @entity1230 and @entity295 at the earliest time points. This study demonstrates that the early leukocyte genomic response to @entity295 and @entity130 are very similar in @entity19 .

PATTERNS OF GENE EXPRESSION AMONG XXXX MODELS OF HEMORRHAGIC SHOCK/TRAUMA AND SEPSIS.

@entity295 :: ('MESH:D018805', 'Disease') :: ['sepsis'] @entity548 :: ('MESH:D006470', 'Disease') :: ['trauma-hemorrhage'] @entity55 :: ('MESH:D011014', 'Disease') :: ['pneumonia'] @entity19 :: ('10090', 'Species') :: ['mice', 'murine'] @entity12147 :: ('12950(Tax:10090)', 'Gene') :: ['CLP'] @entity1230 :: ('MESH:D009104', 'Disease') :: ['polytrauma'] @entity130 :: ('MESH:D014947', 'Disease') :: ['trauma']
@entity19 :: (10090,Species) :: ['MURINE']
