Superparamagnetic @entity1933 nanoparticles (SPIONs) are frequently used for drug targeting, @entity221 and other biomedical purposes. Recently, we have reported the synthesis of @entity27146 -/albumin-coated @entity1933 nanoparticles SEON(LA-BSA), which were synthesized using excess albumin. For optimization of magnetic treatment applications, SPION suspensions need to be purified of excess surfactant and concentrated. Conventional methods for the purification and concentration of such ferrofluids often involve high shear stress and low purification rates for macromolecules, like albumin. In this work, removal of albumin by low shear stress tangential ultrafiltration and its influence on SEON(LA-BSA) particles was studied. Hydrodynamic size, surface properties and, consequently, colloidal stability of the nanoparticles remained unchanged by filtration or concentration up to four-fold (v/v). Thereby, the saturation magnetization of the suspension can be increased from 446.5 A/m up to 1667.9 A/m. In vitro analysis revealed that cellular uptake of SEON(LA-BSA) changed only marginally. The specific absorption rate (SAR) was not greatly affected by concentration. In contrast, the maximum temperature Tmax in magnetic @entity221 is greatly enhanced from 44.4   C up to 64.9   C by the concentration of the particles up to 16.9 mg/mL total @entity68 . Taken together, tangential ultrafiltration is feasible for purifying and concentrating complex hybrid coated SPION suspensions without negatively influencing specific particle characteristics. This enhances their potential for magnetic treatment.

Tangential Flow Ultrafiltration Allows Purification and Concentration of XXXX -/Albumin-Coated Particles for Improved Magnetic Treatment.

@entity221 :: ('MESH:D005334', 'Disease') :: ['hyperthermia'] @entity1933 :: ('MESH:C000499', 'Chemical') :: ['iron oxide'] @entity68 :: ('MESH:D007501', 'Chemical') :: ['iron'] @entity27146 :: ('MESH:C030358', 'Chemical') :: ['lauric acid']
@entity27146 :: (MESH:C030358,Chemical) :: ['Lauric Acid']
