In @entity694 , endothelial cell selectins can promote @entity5 cell attachment via interactions with sialylated Lewis antigens present at the surface of @entity5 cells, thereby facilitating @entity5 cell arrest and transmigration into the extravascular space. However, it is not known whether Lewis antigens interact with @entity694 cells and modify their migration. Our aim was to detect the presence of binding sites on @entity1 @entity5 cells for Lewis(a/x) antigens and their sialylated derivatives in vitro and in vivo and to analyze their influence on migration of @entity694 cells. The immunocytochemical and histochemical levels of expression of the four Lewis antigens were quantitatively determined in four @entity1 @entity694 cell lines and in in vivo @entity19 xenografts. The levels of expression of specific binding sites for these sugar epitopes were determined by synthetic neoglycoconjugates. The influence of binding of these @entity1735 ligands on @entity5 cell migration was quantitatively evaluated by computer-assisted phase-contrast videomicroscopy performed on Matrigel culture supports either left uncoated or coated with neoglycoconjugate presenting synthetic Lewis(a), sialyl Lewis(a), Lewis(x), or sialyl Lewis(x) antigens. The influence of the @entity460 concentration in the culture medium on the Lewis antigen-mediated effects was checked. @entity1 @entity694 cells expressed significant amounts of specific binding sites detected by the synthetic probes in addition to the oligosaccharide epitopes. The expression levels differed considerably between the four cell lines and between in vitro and in vivo specimens. Cell migration analysis revealed that the four Lewis antigens markedly decreased the levels of migration of the HCT-15 and LoVo @entity5 cells. This effect depends on the @entity460 concentration in the culture medium. Binding sites for @entity1832 are present on @entity694 cells. The functional relevance of these sites is indicated by the negative influence on cell migration of a matrix containing the oligosaccharides as ligand parts.

Binding sites for Lewis antigens are expressed by @entity1 XXXX cells and negatively affect their migration.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity19 :: ('10090', 'Species') :: ['nude mice'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['carbohydrate'] @entity1832 :: ('MESH:D020961', 'Disease') :: ['Lewis epitopes'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon tumor', 'colon cancer']
@entity694 :: (MESH:D003110,Disease) :: ['colon cancer']
