The assembly of @entity29535 depends on bidirectional intraflagellar transport (IFT). Anterograde IFT is driven by kinesin II, whereas retrograde IFT requires cytoplasmic dynein 1b (cDHC1b). Little is known about how cDHC1b interacts with its cargoes or how it is regulated. Recent work identified a novel dynein light intermediate chain ( @entity69634 ) that colocalized with the @entity1 cDHC1b homolog @entity27272 in the centrosomal region of cultured cells. To see whether the LIC might play a role in IFT, we characterized the gene encoding the Chlamydomonas homolog of @entity69634 and found its expression is up-regulated in response to deflagellation. We show that the LIC subunit copurifies with cDHC1b during flagellar isolation, dynein extraction, @entity969 density centrifugation, and immunoprecipitation. Immunocytochemistry reveals that the LIC colocalizes with cDHC1b in the basal body region and along the @entity3656 in wild-type cells. Localization of the complex is altered in a collection of retrograde IFT and length control mutants, which suggests that the affected gene products directly or indirectly regulate cDHC1b activity. The @entity1 @entity27272 and @entity69634 also colocalize in the apical cytoplasm and axonemes of ciliated epithelia in the lung, brain, and efferent duct. These studies, together with the identification of an LIC mutation, @entity69635 ), which disrupts retrograde IFT in @entity2199 , indicate that the novel LIC is a component of the cDHC1b/ @entity27272 retrograde IFT motor in a variety of organisms.

A novel dynein light intermediate chain colocalizes with the retrograde motor for intraflagellar transport at sites of axoneme assembly in chlamydomonas and XXXX cells.

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity27272 :: ('79659', 'Gene') :: ['DHC2'] @entity3656 :: ('MESH:D007870', 'Disease') :: ['length of flagella'] @entity2199 :: ('6239', 'Species') :: ['Caenorhabditis elegans'] @entity69634 :: ('51626', 'Gene') :: ['D2LIC'] @entity29535 :: ('MESH:C536287', 'Disease') :: ['cilia and flagella'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity69635 :: ('184080(Tax:6239)', 'Gene') :: ['xbx-1(ok279']
@entity1 :: (9606,Species) :: ['Mammalian']
