We compared @entity1198 and Th2 cytokines secreted by randomly selected, intrathyroidal CD4+ T cell clones isolated from a @entity1 with @entity2743 using @entity3646 + @entity1199 versus @entity1199 alone. Prior to T cell isolation, PCR of cDNA from the intact thyroid tissue generated @entity3646 and @entity688 , but not @entity379 , products. As controls, @entity3646 , @entity379 and @entity688 cDNA was amplified from stimulated, but not unstimulated, PBMC. All 21 of the nine @entity1199 clones and twelve @entity1199 + @entity3646 clones isolated from the thyroid tissue were CD4+. With the exception of one clone in the @entity1199 group, all clones produced @entity688 on stimulation with anti-CD3 and @entity1359 ( @entity4515 ) with similar mean values for both groups. The majority of clones in both groups also produced @entity379 and @entity3646 after stimulation. However, the @entity3646 : @entity379 ratios were significantly higher in clones isolated using @entity1199 + @entity3646 than in those isolated with @entity1199 alone. Furthermore, the distribution of @entity1198 , Th0 and Th2 type clones, defined by their @entity3646 : @entity379 ratios, was also significantly different between those isolated using @entity1199 alone and those isolated using @entity1199 + @entity3646 . Of note, although Th0 clones predominated in both groups, @entity1198 clones were only obtained using @entity1199 alone and Th2 clones were only obtained with @entity1199 + @entity3646 . In conclusion, the presence of @entity3646 together with @entity1199 induces a shift away from a @entity1198 -towards a Th2-response in T cells cloned from in vivo-activated thyroid-infiltrating lymphocytes. This difference in cytokine profile emphasizes that @entity3646 is required for cloning T cells representative of a response involving both @entity1198 and Th2 cells, as occurs in @entity1669 .

Cytokine profiles of in vivo activated thyroid-infiltrating T cells cloned in the presence or absence of XXXX .

@entity1 :: ('9606', 'Species') :: ['patient'] @entity2743 :: ('MESH:D006111', 'Disease') :: ["Graves' disease"] @entity1669 :: ('MESH:D013967', 'Disease') :: ['autoimmune thyroid disease'] @entity4515 :: ('CHEBI:60755', 'Chemical') :: ['PMA'] @entity1198 :: ('51497', 'Gene') :: ['Th1'] @entity1359 :: ('CHEBI:37537', 'Chemical') :: ['phorbol-12-myristate 13-acetate'] @entity379 :: ('3458', 'Gene') :: ['IFN-gamma'] @entity1199 :: ('3558', 'Gene') :: ['IL-2'] @entity3646 :: ('3565', 'Gene') :: ['IL-4'] @entity688 :: ('3586', 'Gene') :: ['IL-10']
@entity3646 :: (3565,Gene) :: ['interleukin 4']
