INTRODUCTION: @entity86 continue to be a major source of morbidity and occasional mortality. Studies have shown that matrix metalloproteinase-9 (MMP-9) levels are decreased postoperatively which may limits matrix degradation and participate in the development of @entity298 . In this proof-of-principle study, we evaluated the effect of gene therapy with catalytically inactive mutant MMP-9 on @entity86 in @entity35 . METHODS: Adenovirus encoding mutant MMP-9 (Ad-mMMP-9) or saline was instilled in the peritoneal cavity after cecal and parietal peritoneal injury in @entity35 . Expression of mutant MMP-9 transcript was verified by sequencing. Adenovirus E4 gene expression, adhesion scores, MMP-9, @entity2435 (tPA), @entity2630 ( @entity2630 ) and @entity1810 ( @entity1810 ) expression were evaluated at sacrifice one week after treatment. RESULTS: Both mutant MMP-9 transcripts and adenovirus E4 gene were expressed in Ad-mMMP-9 treated adhesions. Adhesions severity decreased significantly (p  =  0.036) in the Ad-mMMP-9-treated compared with saline-treated adhesions. Expression of MMP-9 mRNA and protein were elevated (p = 0.001 andp  =  0.029, respectively) in the Ad-mMMP-9-treated adhesions compared with saline-treated adhesions. While tPA levels were increased (p  =  0.02) in Ad-mMMP-9 treated adhesions compared with saline-treated adhesions, @entity1810 and @entity2630 levels were decreased (p  =  0.017 and p  =  0.042, respectively). No difference in mortality were found between groups (p =  0.64). CONCLUSIONS: Mutant MMP-9 gene therapy effectively transduced @entity298 resulting in reduction of severity of @entity298 .

Mutant matrix metalloproteinase-9 reduces XXXX in @entity35 .

@entity2630 :: ('24617(Tax:10116)', 'Gene') :: ['PAI-1', 'plasminogen activator inhibitor-1'] @entity2435 :: ('25692(Tax:10116)', 'Gene') :: ['tissue plasminogen activator'] @entity86 :: ('MESH:D019106', 'Disease') :: ['Postoperative peritoneal adhesions', 'postoperative peritoneal adhesions'] @entity1810 :: ('59086(Tax:10116)', 'Gene') :: ['transforming growth factor-b1', 'TGF-b1'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity298 :: ('MESH:D010538', 'Disease') :: ['peritoneal adhesions', 'primary peritoneal adhesions']
@entity86 :: (MESH:D019106,Disease) :: ['postoperative peritoneal adhesions']
