Hepatocyte transplantation is an alternative therapy to orthotopic liver transplantation for the treatment of @entity409 . Good quality freshly isolated or cryopreserved @entity1 hepatocytes are needed for clinical transplantation. However, isolation, cryopreservation, and thawing processes can seriously impair hepatocyte viability and functionality. The aim of the present study was to develop a fast and sensitive procedure to estimate the quality of hepatocyte preparations prior to clinical cell infusion. To this end, cell viability, attachment efficiency, and metabolic competence ( @entity1133 synthesis and drug-metabolizing P450 activities) were selected as objective criteria. Viability of hepatocyte suspension was estimated by @entity3460 staining. DNA content of attached cells 50 min after hepatocyte platting to fibronectin/collagen-coated dishes was quantified to estimate adherence capacity. Urea production was determined after incubating hepatocyte suspensions with 2 mM C1NH4 for 30 min. The cytochrome P450 function was assayed by a 30-min incubation of hepatocyte suspension with a cocktail mixture containing selective substrates for seven individual P450 activities ( @entity4914 , 2A6, 2C9, 2C19, 2D6, 2E1, and 3A4). The assay can be applied to both freshly isolated and cryopreserved hepatocyte suspensions, and the results are available within 1 h, which could help to make short-term decisions: 1) to assess the suitability for cell transplantation of a preparation of freshly isolated hepatocytes or a particular batch of thawed cells, or 2) to estimate the convenience of banking a particular cell preparation.

Functional assessment of the quality of XXXX hepatocyte preparations for cell transplantation.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1133 :: ('MESH:D014508', 'Chemical') :: ['urea'] @entity409 :: ('MESH:D008107', 'Disease') :: ['liver diseases'] @entity3460 :: ('MESH:D014343', 'Chemical') :: ['trypan blue'] @entity4914 :: ('1544', 'Gene') :: ['CYP1A2']
@entity1 :: (9606,Species) :: ['human']
