BACKGROUND/AIMS: Paraffin embedded samples have provided an important source of material for retrospective cytofluorimetric studies, useful in establishing the predictive value of DNA content measurements. The aim of this study was to investigate the incidence and type of aneuploidy in @entity320 ( @entity320 ) and the significance in the clinical outcome (median follow up 55 months). METHODS: DNA content was quantified by flow cytometry in 61 @entity320 from archival material. @entity5 ocular tissue was used as the reference diploid standard. Cases in which the coefficient of variation (CV) of the diploid peak was > 8% were excluded. The @entity320 were classified as spindle A, spindle B, mixed spindle and epithelioid, epithelioid, and @entity39 . RESULTS: The frequency of the aneuploid DNA pattern was 38%. @entity5 showed a worse clinical outcome independent of the ploidy pattern. Spindle A @entity5 were found to be diploid. Spindle B and mixed @entity5 showed a prevalent diploid and near diploid aneuploid pattern (DI < 1.3), yet aneuploidy was not correlated with a worse prognosis. The epithelioid @entity5 were prevalently diploid. However, 83% of the @entity5 were hypodiploid (DI < 0.95), and showed the worst prognosis. CONCLUSION: These results indicate that increasing DNA abnormalities in @entity320 , especially in the epithelioid histotype, were associated with an increasing mortality.

DNA ploidy pattern in XXXX : correlation with survival. A flow cytometry study on archival material.

@entity5 :: ('MESH:D009369', 'Disease') :: ['Necrotic tumours', 'Non-tumour', 'tumours', 'aneuploid tumours'] @entity320 :: ('MESH:D008545', 'Disease') :: ['CMM', 'choroidal malignant melanomas'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrotic']
@entity320 :: (MESH:D008545,Disease) :: ['choroidal melanoma']
