UNASSIGNED: The hedgehog (Hh) signaling pathway plays fundamental roles during embryonic development and tumorigenesis. Previously, we have shown that ablation of the tumor suppressor and negative regulator, Suppressor of fused ( @entity39597 ), within this pathway causes embryonic lethality around E9.5 in the @entity19 . In this study, we examine how lack of @entity39597 influences early cell fate determination processes. We established embryonic stem cell (ESC) lines from preimplantation @entity39597 (-/-) and wild-type @entity19 embryos and show that these ESCs express the typical pluripotency markers, alkaline phosphatase, @entity11823 , @entity7002 , @entity14231 , and Nanog. We demonstrate that these ESCs express all core Hh pathway components and that @entity19990 mRNA levels are increased in @entity39597 (-/-) ESCs. Upon spontaneous differentiation of @entity39597 (-/-) ESCs into embryoid bodies (EBs) in vitro, the Hh pathway is strongly upregulated as indicated by an increase in both @entity19990 and @entity20735 ( @entity20735 ) gene expression. Interestingly, developing @entity39597 (-/-) EBs were smaller than their wild-type counterparts and showed decreased expression of the ectodermal markers, @entity46000 and @entity24390 . In vivo teratoma formation revealed that @entity39597 (-/-) ESCs have a limited capacity for differentiation as the resulting tumors lacked the mesodermal derivatives, cartilage and bone. However, @entity39597 (-/-) ESCs were able to develop into chondrocytes and osteocytes in vitro, which suggests a differential response of ESCs compared with in vivo conditions. Our findings suggest a regulatory function of the Hh signaling pathway in early mesodermal cell fate determination and emphasize the role of @entity39597 as a key molecule in this process.

Suppressor of Fused Plays an Important Role in Regulating Mesodermal Differentiation of XXXX Embryonic Stem Cells In Vivo.

@entity14231 :: ('20674(Tax:10090)', 'Gene') :: ['Sox2'] @entity11823 :: ('14345(Tax:10090)', 'Gene') :: ['SSEA-1'] @entity24390 :: ('20664(Tax:10090)', 'Gene') :: ['Sox1'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity39597 :: ('24069(Tax:10090)', 'Gene') :: ['Sufu'] @entity19990 :: ('14632(Tax:10090)', 'Gene') :: ['Gli1', 'glioma-associated protein (Gli)1'] @entity7002 :: ('100846986', 'Gene') :: ['Oct4'] @entity20735 :: ('19206(Tax:10090)', 'Gene') :: ['patched1', 'Ptch1'] @entity46000 :: ('14176(Tax:10090)', 'Gene') :: ['Fgf5']
@entity19 :: (10090,Species) :: ['Murine']
