Social isolation stress (SIS) in adolescence is accompanied by @entity4934 and pathophysiological changes in certain regions of the CNS such as the hippocampus. In this study, we tested whether SIS impacts @entity410 susceptibility in postnatal male @entity19 due to a role of hippocampal @entity842 (NO). To do this, we used the @entity5093 (PTZ) model of @entity410 , open-field test, hole-board test, forced swimming test, and plasma @entity2819 assay. We aimed to evaluate if 4weeks of SIS is capable of decreasing @entity410 threshold along with altering affective and neuroendocrine responses in isolated conditioned (IC) animals in comparison with socially conditioned (SC) animals. In addition, we applied subeffective doses of NO precursor @entity534 (25, 50, and 100mg/kg) and NOS inhibitors 7-NI (15 and 40mg/kg), @entity5118 (50 and 100mg/kg), and l-NAME (10 and 15mg/kg) to both IC and SC groups prior to the determination of @entity410 threshold. Injection of a single dose of all mentioned drugs did not induce changes in @entity410 threshold of SC @entity19 . On the other hand, l-NAME and 7-NI, but not @entity5118 , modulated the proconvulsant effect of SIS, while @entity534 augmented the latter effect. We also measured the hippocampal @entity3542 levels after the administration of the aforementioned drugs. Social isolation stress increased the @entity3542 levels in comparison with those in SC @entity19 , whereas 7-NI and l-NAME, unlike @entity5118 , mitigated the effect of SIS. Additionally, @entity534 boosted the effects of SIS on @entity3542 production. In summary, we showed that SIS enhanced @entity410 susceptibility in the PTZ model of @entity410 through the activation of the nitrergic system in the hippocampus. Also, we proved that @entity17693 , but not iNOS, accounts for these changes following SIS.

Involvement of the nitrergic system in the proconvulsant effect of social isolation stress in male XXXX .

@entity410 :: ('MESH:D012640', 'Disease') :: ['seizure', 'clonic seizures'] @entity5118 :: ('MESH:C004479', 'Chemical') :: ['aminoguanidine'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity3542 :: ('CHEBI:16301', 'Chemical') :: ['nitrite'] @entity4934 :: ('MESH:D019954', 'Disease') :: ['neurobehavioral disturbances'] @entity534 :: ('CHEBI:16467', 'Chemical') :: ['l-arginine'] @entity2819 :: ('MESH:D003345', 'Chemical') :: ['corticosterone'] @entity17693 :: ('18125(Tax:10090)', 'Gene') :: ['nNOS'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity5093 :: ('MESH:D010433', 'Chemical') :: ['pentylenetetrazole']
@entity19 :: (10090,Species) :: ['mice']
