AIM: To investigate the role of NF-kappaB activation and @entity219 finger protein A20 expression in the regulation of maturation of dendritic cells (DCs) derived from liver allografts undergoing acute rejection. METHODS: Sixty donor male SD @entity35 and sixty recipient male LEW @entity35 weighing 220-300 g were randomly divided into whole liver transplantation group and partial liver transplantation group. Allogeneic (SD @entity35 to LEW @entity35 ) whole and 50 % partial liver transplantation were performed. DCs from liver grafts 0 hour and 4 days after transplantation were isolated and propagated in the presence of @entity7421 in vitro. Morphological characteristics and phenotypical features of DCs propagated for 10 days were analyzed by electron microscopy and flow cytometry, respectively. NF-kappaB binding activity, IL-12p70 protein and @entity219 finger protein A20 expression in these DCs were measured by EMSA and Western blotting, respectively. Histological grading of rejection was determined. RESULTS: Allogeneic whole liver grafts showed no signs of rejection on day 4 after the transplantation. In contrast, allogeneic partial liver grafts demonstrated moderate to severe rejection on day 4 after the transplantation. After propagation for 10 days in the presence of @entity7421 in vitro, DCs from allogeneic whole liver grafts exhibited features of immature DC with absence of @entity23655 surface expression, these DCs were found to exhibit detectable but very low level of NF-kappaB activity, IL-12 p70 protein and @entity219 finger protein A20 expression. Whereas, DCs from allogeneic partial liver graft 4 days after transplantation displayed features of mature DC, with high level of @entity23655 surface expression, and as a consequence, higher expression of IL-12p70 protein, higher activities of NF-kappaB and higher expression of @entity219 finger protein A20 compared with those of DCs from whole liver grafts (P<0.001). CONCLUSION: These results suggest that A20 expression is up-regulated in response to NF-kappaB activation in mature DCs derived from allogeneic liver grafts undergoing acute rejection. Given the NF-kappaB inhibition function of this gene, it is suggested that their expression survives to limit NF-kappaB activation and maturation of DCs, and consequently inhibits the acute rejection and induces acceptance of liver graft.

NF-kappaB activation and XXXX finger protein A20 expression in mature dendritic cells derived from liver allografts undergoing acute rejection.

@entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity7421 :: ('116630(Tax:10116)', 'Gene') :: ['GM-CSF'] @entity219 :: ('MESH:D015032', 'Chemical') :: ['zinc'] @entity23655 :: ('171369(Tax:10116)', 'Gene') :: ['CD40']
@entity219 :: (MESH:D015032,Chemical) :: ['zinc']
