1. The effects of phosphodiesterase (PDE) inhibition and @entity1928 pretreatment on the contractile responses of @entity840 urinary bladder strips to electrical field stimulation, @entity5402 , @entity855 and @entity4338 were studied. 2. Inhibition of @entity1919 -specific PDE4 isozymes by @entity7428 significantly reduced the contractile response of bladder strips to field stimulation. @entity7428 also suppressed the contractile response to low concentrations of @entity5402 , but potentiated the response to high concentrations. The contractile response to @entity855 was significantly reduced by @entity7428 treatment, but that to @entity4338 was unaltered. 3. Inhibition of @entity8262 -specific PDE5 isozymes by zaprinast had no effects on the contractile response of bladder strips to field stimulation, @entity855 or @entity4338 . @entity10987 suppressed the contractile responses to 1 microM @entity5402 and potentiated the response to high concentrations. 4. Contractile responses to field stimulation and to @entity5402 after pretreatment with the adenylyl cyclase activator, @entity1928 , were qualitatively similar to those caused by @entity7428 treatment. beta-Adrenoceptor blockade with @entity1169 partially reversed the inhibitory effects of @entity7428 on the response to field stimulation. 5. @entity7428 significantly reduced the contractile response of bladder strips from sensitized @entity840 to ovalbumin challenge, but zaprinast was ineffective. PDE inhibition had similar effects on the responsiveness of control and of sensitized @entity840 bladder strips to field stimulation, @entity5402 , @entity855 and @entity4338 . 6. The data suggest that the contractile response of @entity840 bladder strips can be modified by increases in @entity1919 levels.

The role of cyclic nucleotides in XXXX bladder contractility.

@entity10987 :: ('MESH:C011145', 'Chemical') :: ['Zaprinast'] @entity1169 :: ('MESH:D011433', 'Chemical') :: ['propranolol'] @entity7428 :: ('MESH:D020889', 'Chemical') :: ['Rolipram', 'rolipram'] @entity840 :: ('10141', 'Species') :: ['guinea-pig', 'guinea-pigs'] @entity8262 :: ('MESH:D006152', 'Chemical') :: ['cyclic GMP'] @entity1919 :: ('MESH:D000242', 'Chemical') :: ['cyclic AMP'] @entity1928 :: ('MESH:D005576', 'Chemical') :: ['forskolin'] @entity5402 :: ('MESH:D002217', 'Chemical') :: ['carbachol'] @entity4338 :: ('CHEBI:32588', 'Chemical') :: ['KCl'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity840 :: (10141,Species) :: ['guinea-pig']
