OBJECTIVE: Approximately one third of @entity1 diagnosed with muscle-invasive @entity150 ( @entity150 ) have undetected metastases at the time of treatment of the @entity5 . Currently there are no reliable specific serum markers for monitoring and evaluating risk profiles of @entity5 . Several studies suggest that detection of circulating @entity5 cells (CTCs) may correlate with the disease status and prognosis at baseline and early in the treatment of @entity5 . In this study a new way of isolation and in vitro cultivation of CTCs of @entity150 was introduced. MATERIALS AND METHODS: Peripheral blood (PB) samples from 53 @entity1 who had undergone urological procedure were evaluated using the MetaCell device (MetaCell s.r.o., Ostrava, Czech Republic). The @entity1 enrolled in the study were both oncological @entity1 with @entity150 and non-oncological @entity1 with @entity32 (14 @entity1 ). The sensitivity and quantification of CTCs were evaluated. The separated CTCs were cultured in vitro. RESULTS: 39 @entity1 with confirmed @entity150 were enrolled in the study. CTCs were detected in 25 (64%) @entity1 , and most of these @entity1 had between 6 and 10 cells. The separated CTCs were successfully cultured in vitro. CONCLUSION: CTCs were detected in a higher percentage of @entity1 than in other studies. This paper describes the first successful culturing of @entity1 @entity150 cells. The MetaCell approach used in this study enabled the capture of viable intact virgin CTCs (virgin CTC) suitable for next in vitro culturing, single cell analysis or drug testing.

In vitro culturing of viable circulating XXXX cells of @entity150 .

@entity1 :: ('9606', 'Species') :: ['patients', 'human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity150 :: ('MESH:D001749', 'Disease') :: ['UBC', 'urinary bladder cancer'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'urothelial cancers', 'cancers', 'primary tumor']
@entity5 :: (MESH:D009369,Disease) :: ['tumor']
