Adaptive mutation refers to the continuous outgrowth of new mutants from a non-dividing cell population during selection, in apparent violation of the neo-Darwinian principle that mutation precedes selection. One explanation is that of retromutagenesis, in which a DNA lesion causes a transcriptional mutation that yields a mutant protein, allowing escape from selection. This enables a round of DNA replication that establishes heritability. Because the model requires that gene expression precedes DNA replication, it predicts that during selection, new mutants will arise from damage only to the transcribed DNA strand. As a test, we used a lacZ amber mutant of @entity526 that can revert by @entity17241 -induced deamination of @entity5536 residues on either strand of the TAG stop codon, each causing different DNA mutations. When stationary-phase, mutagenized cells were grown in rich broth before being plated on @entity1132 -selective media, only non-transcribed strand mutations appeared in the revertants. This result was consistent with the known high sensitivity to deamination of the single-stranded DNA in a transcription bubble, and it provided an important control because it demonstrated that the genetic system we would use to detect transcribed-strand mutations could also detect a bias toward the non-transcribed strand. When residual lacZ transcription was blocked beforehand by catabolite repression, both strands were mutated about equally, but if revertants were selected immediately after @entity17241 exposure, transcribed-strand mutations predominated among the revertants, implicating retromutagenesis as the mechanism. This result was not affected by gene orientation. Retromutagenesis is apt to be a universal method of evolutionary adaptation, which enables the emergence of new mutants from mutations acquired during counterselection rather than beforehand, and it may have roles in processes as diverse as the development of antibiotic resistance and @entity5 .

Evidence for Retromutagenesis as a Mechanism for Adaptive Mutation in XXXX .

@entity5 :: ('MESH:D009369', 'Disease') :: ['neoplasia'] @entity1132 :: ('MESH:D007785', 'Chemical') :: ['lactose'] @entity5536 :: ('MESH:D000225', 'Chemical') :: ['adenine'] @entity17241 :: ('MESH:D009608', 'Chemical') :: ['nitrous acid'] @entity526 :: ('562', 'Species') :: ['Escherichia coli']
@entity526 :: (562,Species) :: ['Escherichia coli']
