We have developed an animal model to study @entity1 @entity56 reactions. Previous studies in @entity1 have shown after tuberculin injection the presence of a mononuclear cell infiltration, with almost no eosinophils, associated with a preferential Th-1-type cytokine profile. @entity1 skin graft obtained from tuberculin-reactive donors was grafted onto the back of severe combined immunodeficient @entity19 . After healing, @entity19 were reconstituted intraperitoneally with peripheral mononuclear cells. Tuberculin and diluent were injected intradermally, and skin biopsies were performed 72 hours later. Skin grafts were divided into two parts, one for immunohistochemistry and one for in situ hybridization studies. Immunohistochemistry was performed on cryostat sections using the alkaline phosphatase anti-alkaline phosphatase technique. In the tuberculin-injected sites as compared with the diluent-injected sites, there were significant increases in the number of @entity1195 + pan leukocytes and CD4+, CD8+, CD45RO+ T cells but not in @entity469 + monocytes/macrophages and EG2 or MBP+ eosinophils. The activation markers @entity2902 and HLA-DR were up-regulated in the tuberculin-injected sites. In situ hybridization was performed using 35S-labeled riboprobes for interleukin (IL)-2, interferon (IFN)-gamma, @entity3646 , and @entity225 . After tuberculin injection, a preferential Th-1-type cytokine profile was observed with significant increases in the numbers of @entity1199 and @entity379 mRNA-expressing cells. These results are similar to those reported after tuberculin-induced @entity56 in @entity1 , suggesting that this model might be useful to study cutaneous inflammatory reaction.

Tuberculin-induced @entity56 reaction in a model of hu-PBMC-SCID XXXX grafted with autologous skin.

@entity1 :: ('9606', 'Species') :: ['humans', 'human', 'Human'] @entity225 :: ('3567', 'Gene') :: ['IL-5'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity469 :: ('968', 'Gene') :: ['CD68'] @entity1195 :: ('5788', 'Gene') :: ['CD45'] @entity56 :: ('MESH:D004342', 'Disease') :: ['delayed-type hypersensitivity'] @entity379 :: ('3458', 'Gene') :: ['IFN-gamma'] @entity2902 :: ('3559', 'Gene') :: ['CD25'] @entity1199 :: ('3558', 'Gene') :: ['IL-2'] @entity3646 :: ('3565', 'Gene') :: ['IL-4']
@entity19 :: (10090,Species) :: ['mice']
