UNASSIGNED: @entity18896 (MVC) is an autonomous parvovirus in the Bocaparvovirus genus. It has a single promoter that generates a single pre-mRNA processed via alternative splicing and alternative polyadenylation to produce at least 8 mRNA transcripts. MVC contains two polyadenylation sites, one at the right-hand end of the genome, (pA)d, and another complex site, (pA)p, within the capsid-coding region. During @entity1800 , the mRNAs must extend through (pA)p and undergo additional splicing of the immediately-upstream 3D/3A intron to access the capsid gene. MVC @entity11915 is a 22 kDa nuclear phosphoprotein unique to the Bocaparvovirus genus of the Parvovirinae which we have shown governs suppression of (pA)p independently of viral genome replication. We show here that in addition to suppression of (pA)p, @entity11915 is also required for the excision of the MVC 3D/3A intron, independently of its effect on alternative polyadenylation. Mutations of the @entity369 / @entity563 (SR) di-repeats within the intrinsically disordered @entity1472 -terminus of @entity11915 are required for splicing of the capsid transcript but not suppression of polyadenylation at (pA)p. 3' -end processing of MVC mRNAs at (pA)p is critical for viral genome replication and the optimal expression of @entity11915 and @entity8768 . Thus, a finely-tuned balance between (pA)p suppression and usage is necessary for efficient virus replication. @entity11915 is the first parvovirus protein implicated in RNA processing. Its characterization reveals another way that parvoviruses govern access to their capsid-protein genes, namely, at the RNA level, by regulating the essential splicing of an intron and the suppression of an internal polyadenylation site. IMPORTANCE: The Parvovirinae are small non-enveloped icosahedral viruses that are important pathogens in many animal species including @entity1 . Although parvoviruses have only subtle early-to-late expression shifts, they all regulate access to their capsid genes. @entity18896 (MVC) is an autonomous parvovirus in the Bocaparvovirus genus. It has a single promoter generating a single pre-mRNA which is processed via alternative splicing and alternative polyadenylation to generate at least 8 mRNA transcripts. MVC contains two polyadenylation sites, one at the right-hand end of the genome, (pA)d, and another, (pA)p, within the capsid-coding region. It had not been clear how the potent internal polyadenylation motif is suppressed to allow processing, export, and accumulation of the spliced capsid protein-encoding mRNAs. We show here that MVC @entity11915 , the first parvovirus protein to be implicated in RNA processing, governs access to the MVC capsid gene by facilitating splicing and suppressing internal polyadenylation of MVC pre-mRNAs.

The Bocaparvovirus XXXX NP1 protein controls access to the viral capsid genes via its role in RNA processing.

@entity1 :: ('9606', 'Species') :: ['humans'] @entity1472 :: ('CHEBI:46882', 'Chemical') :: ['amino'] @entity8768 :: ('10625', 'Gene') :: ['NS1'] @entity11915 :: ('4884', 'Gene') :: ['NP1'] @entity1800 :: ('MESH:D014777', 'Disease') :: ['viral infection'] @entity563 :: ('CHEBI:17822', 'Chemical') :: ['serine'] @entity18896 :: ('329639', 'Species') :: ['Minute virus of canines'] @entity369 :: ('CHEBI:29016', 'Chemical') :: ['arginine']
@entity18896 :: (329639,Species) :: ['Minute Virus of Canines']
