A role for the insulin-like growth factors (IGFs) in brain growth and differentiation has recently been suggested. In previous studies on fetal hypothalamic cells we found a trophic influence of @entity2135 on in vitro survival and differentiation of both neurons and glia. We have now investigated the expression of @entity2135 , its receptor and its binding proteins in the @entity35 hypothalamus to determine whether endogenous @entity2135 might serve as a trophic factor during development of this brain area. Both @entity2135 receptors and @entity2135 binding proteins showed marked developmental stage-dependent variations. Thus, @entity2135 receptors as measured by both binding and cross-linking techniques, were highest during fetal life and steadily decreased thereafter to reach low adult levels. Changes in receptor numbers rather than in its affinity constant accounted for the differences seen in binding activity during development. In addition, we found 3 different @entity2135 binding proteins (IGFBPs) of apparent Mr of 24, 29 and 32 kDa respectively, whose levels also showed a specific developmental pattern. Highest levels of the 29 and 32 kDA IGFBPs were found in fetal and early postnatal life, whereas levels of the 24 kDa form were highest in young adults. Changes in the concentration of IGFBPs rather than in their affinities for @entity2135 accounted for the different binding capacities found. Using a specific @entity2135 radioimmunoassay we found that @entity2135 -like immunoreactivity ( @entity2135 -li) levels had no direct correlation with developmental stage. @entity2135 -li levels oscillated with no apparent trend throughout development of the hypothalamus.(ABSTRACT TRUNCATED AT 250 WORDS)

Ontogeny of insulin-like growth factor I, its receptor, and its binding proteins in the XXXX hypothalamus.

@entity35 :: ('10116', 'Species') :: ['rat'] @entity2135 :: ('24482(Tax:10116)', 'Gene') :: ['IGF-I']
@entity35 :: (10116,Species) :: ['rat']
