UNASSIGNED: @entity23159 isomerase (IPI) catalyzes the isomerization between the common @entity9534 precursor substances @entity23159 ( @entity34486 ) and @entity34484 ( @entity34484 ) during the @entity25164 biosynthesis process. In this study, tissue expression analysis revealed that the expression level of the Salvia miltiorrhiza IPI1 gene (SmIPI1) was higher in the leaves than in the roots and stems. Furthermore, color complementation and RNA interference methods were used to verify the function of the SmIPI1 gene from two aspects. A recombinant SmIPI1 plasmid was successfully constructed and transferred into engineered @entity526 for validating the function of SmIPI1 through the color difference in comparison to the control group; the observed color difference indicated that SmIPI1 served in promoting the accumulation of @entity14319 . Transformant hairy root lines with RNA interference of SmIPI1 were successfully constructed mediated by @entity7060 ACCC 10060. RNA interference hairy roots had a severe phenotype characterized by withering, @entity204 . The mRNA expression level of SmIPI1 in the RSi3 root line was only 8.4% of that of the wild type. Furthermore the @entity16217 content was too low to be detected in the RNA interference lines. These results suggest that SmIPI1 plays a critical role in @entity25164 metabolic pathways. Addition of an exogenous SmIPI1 gene promoted metabolic flow toward the biosynthesis of carotenoids in @entity526 , and SmIPI1 interference in S. miltiorrhiza hairy roots may cause interruption of the @entity68498 metabolic pathway.

Functional Analysis of the XXXX Isomerase of Salvia miltiorrhiza via Color Complementation and RNA Interference.

@entity34484 :: ('MESH:C043060', 'Chemical') :: ['dimethylallyl diphosphate', 'DMAPP'] @entity23159 :: ('MESH:C004809', 'Chemical') :: ['Isopentenyl diphosphate', 'isopentenyl diphosphate'] @entity9534 :: ('CHEBI:35186', 'Chemical') :: ['terpene'] @entity16217 :: ('MESH:C021751', 'Chemical') :: ['tanshinone'] @entity7060 :: ('359', 'Species') :: ['Agrobacterium rhizogenes'] @entity25164 :: ('CHEBI:26873', 'Chemical') :: ['terpenoid'] @entity14319 :: ('MESH:C015329', 'Chemical') :: ['lycopene'] @entity34486 :: ('MESH:C041272', 'Chemical') :: ['IPP'] @entity204 :: ('MESH:D003643', 'Disease') :: ['deformity or even death'] @entity526 :: ('562', 'Species') :: ['E. coli'] @entity68498 :: ('MESH:C114232', 'Chemical') :: ['2-C-methyl-D-erythritol-4-phosphate']
@entity23159 :: (MESH:C004809,Chemical) :: ['Isopentenyl Diphosphate']
