The @entity1 myeloid nuclear differentiation antigen ( @entity52410 ) is a hematopoietic cell specific nuclear protein. @entity52410 and other related gene products interact with and alter the activity of a large number of proteins involved in regulating specific gene transcription. @entity52410 and related genes exhibit expression characteristics, which suggest functions unique to specific lineages of cells, in addition to mediating the effects of interferons. Cells of the @entity1 K562 myeloid line do not express @entity52410 and are relatively immature compared to lines that express @entity52410 (HL-60, U937, and @entity1357 ). The hypothesis that @entity52410 influences the expression of specific genes was tested by creating @entity52410 expressing K562 cells using stable retroviral mediated gene transfer followed by evaluation of transcription profiles. Two macroarrays containing a total of 2,350 cDNAs of known genes showed a specific up-regulation of @entity11591 expression in @entity52410 expressing K562 cell clones. Real time quantitative RT-PCR analysis confirmed an average of over 3- and 7-fold upregulation of @entity11591 in two clones of @entity52410 expressing K562 cells. The effects on @entity11591 were also confirmed by Northern blotting. @entity11591 is essential for normal hematopoiesis and abnormal expression is a proposed marker of @entity2590 . Additional screening of transcription profiles after induced erythroid and megakaryoblastic differentiation showed no additional gene transcripts altered by the presence of @entity52410 . These results indicate that @entity52410 alters expression of a gene essential for normal hematopoiesis.

Retroviral mediated expression of the XXXX myeloid nuclear antigen in a null cell line upregulates @entity11591 expression.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1357 :: ('2736', 'Gene') :: ['THP1'] @entity11591 :: ('8788', 'Gene') :: ['Dlk1'] @entity2590 :: ('MESH:D009190', 'Disease') :: ['myelodysplastic syndrome'] @entity52410 :: ('4332', 'Gene') :: ['MNDA']
@entity1 :: (9606,Species) :: ['human']
