@entity1 papillomaviruses (HPV) are the causative agents of most cervical @entity189 . A complete understanding of the HPV types that cause @entity2622 is needed as vaccines are designed. Fresh tissues are not always available for such studies. We therefore sought to determine the feasibility of HPV studies using @entity406 -fixed, @entity982 -embedded sections of 56 cervical @entity189 , correlating typing information with the pathology and physical state of the HPV sequences within cells. Sections from each specimen were used to extract and purify DNA. Specific HPV types were identified using a PCR/reverse blot strip assay. Tyramide signal-amplified, fluorescent DNA in situ hybridization (FISH) was used to localize HPV within cells. @entity1 @entity5358 sequences were amplified in DNA from all specimens. HPV sequences from oncogenic types were identified in 52 of 56 (92.9%) by PCR/reverse blot strip assay, and in one additional case using an @entity1799 multiplex PCR assay. @entity1799 was the most commonly detected type, present in most cases as a solitary isolate. Thirty- five of 42 @entity1799 or HPV 18 PCR-positive specimens were also positive in the FISH assay, in most cases in a pattern consistent with viral integration. We conclude that HPV typing from @entity406 -fixed, @entity982 -embedded sections of cervical @entity189 is possible, with a sensitivity that is similar to that found in studies using fresh tissue.

Detection of specific human papillomavirus types in XXXX -embedded sections of cervical @entity189 .

@entity1 :: ('9606', 'Species') :: ['Human'] @entity406 :: ('MESH:D005557', 'Chemical') :: ['formalin'] @entity2622 :: ('MESH:D002583', 'Disease') :: ['cervical carcinoma'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinomas'] @entity982 :: ('MESH:D010232', 'Chemical') :: ['paraffin'] @entity1799 :: ('333760', 'Species') :: ['HPV 16'] @entity5358 :: ('3043', 'Gene') :: ['beta-globin']
@entity982 :: (MESH:D010232,Chemical) :: ['paraffin']
