Blockade of mitogen-activated protein kinase kinase ( @entity16629 ), part of the @entity3963 ( @entity3963 ) or p44/42 mitogen-activated protein kinase (MAPK) pathway has been shown, in some instances, to cause apoptosis in @entity1778 blast cells. However, studies are contradictory and have often been based mainly on inhibition of cell growth in a limited number of cell lines. This investigation examined the effect of the potent @entity8047 inhibitor @entity5732 alone and in combination with @entity2592 on apoptosis in @entity3052 ( @entity3052 ) cell lines, @entity1 acute @entity1778 and nonleukemic samples. Apoptosis was assessed flow cytometrically using @entity3048 .7 and AnnexinV antibodies which detect apoptosis at the mitochondrial and cell membrane levels, respectively. The proapoptotic effect of the inhibitor varied across the five cell lines tested, from highly significant induction of apoptosis to no apparent response. A possible synergistic effect with the combined use of @entity5732 and @entity2592 was observed in one cell line only. The proapoptotic effect of @entity5732 in the most sensitive cell line appeared to be related to @entity2280 positivity. Cells from @entity1778 @entity1 showed considerable sensitivity in two of four cases with a similar association with @entity2280 expression being evident. Interestingly, control cells did not show a significant effect when exposed to the inhibitor. These results suggest that @entity5732 may offer a potential alternative to standard chemotherapy with a particular role in the most primitive types of @entity1778 , these being often the most resistant to standard chemotherapy.

An investigation of the effects of the XXXX inhibitor @entity5732 on apoptosis in @entity3052 .

@entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity8047 :: ('5609', 'Gene') :: ['MEK'] @entity5732 :: ('MESH:C113580', 'Chemical') :: ['U0126'] @entity3963 :: ('5594', 'Gene') :: ['extracellular signal-regulated kinase', 'ERK'] @entity3048 :: ('8797', 'Gene') :: ['Apo2'] @entity2592 :: ('MESH:D003561', 'Chemical') :: ['Ara-C'] @entity16629 :: ('5604;5605', 'Gene') :: ['MEK1/2'] @entity3052 :: ('MESH:D015470', 'Disease') :: ['acute myeloblastic leukemia', 'AML'] @entity1778 :: ('MESH:D007938', 'Disease') :: ['leukemia', 'leukemic'] @entity2280 :: ('947', 'Gene') :: ['CD34']
@entity8047 :: (5609,Gene) :: ['MEK']
