PURPOSE: @entity8185 ( @entity4601 ) has recently shown antitumor activity in @entity1 @entity788 cells. However, its effect on antiangiogenic activity in @entity788 is unknown, and the mechanism is unclear. This study was aimed to investigate whether @entity4601 would inhibit angiogenesis in @entity1 @entity788 . METHODS: Cell viability and proliferation, tube formation of @entity1 umbilical vein endothelial cells (HUVECs), nuclear factor (NF)-kB DNA-binding activity, expressions of @entity7 ( @entity7 ), @entity687 , @entity2247 , and @entity1495 were examined in vitro. The effect of @entity4601 on antiangiogenic activity in @entity788 was also assessed using BxPC-3 xenografts subcutaneously established in BALB/c @entity19 . RESULTS: @entity4601 inhibited cell proliferation and tube formation of HUVECs in a time- and dose-dependent manner and also reduced cell viability in @entity788 cells. @entity4601 significantly inhibited NF-kB DNA-binding activity, so as to tremendously decrease the expression of NF-kB-targeted proangiogenic gene products: @entity7 , @entity687 , @entity2247 , and @entity1495 in vitro. In vivo studies, @entity4601 remarkably reduced @entity5 volume, decreased microvessel density, and down-regulated the expression of NF-kB-related proangiogenic gene products. CONCLUSIONS: Inhibition of NF-kB activation is one of the mechanisms that @entity4601 inhibits angiogenesis in @entity1 @entity788 . We also suggest that @entity4601 could be developed as a novel agent against @entity788 .

XXXX inhibits angiogenesis in @entity788 by targeting the NF-kB pathway.

@entity1 :: ('9606', 'Species') :: ['human'] @entity788 :: ('MESH:D010190', 'Disease') :: ['pancreatic cancer'] @entity7 :: ('7422', 'Gene') :: ['vascular endothelial growth factor', 'VEGF'] @entity19 :: ('10090', 'Species') :: ['nude mice'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity8185 :: ('MESH:C039060', 'Chemical') :: ['Dihydroartemisinin'] @entity4601 :: ('CHEBI:16016', 'Chemical') :: ['DHA'] @entity1495 :: ('4318', 'Gene') :: ['MMP-9', 'matrix metalloproteinase (MMP)-9'] @entity2247 :: ('4513', 'Gene') :: ['cyclooxygenase (COX)-2', 'COX-2'] @entity687 :: ('3576', 'Gene') :: ['interleukin (IL)-8', 'IL-8']
@entity8185 :: (MESH:C039060,Chemical) :: ['Dihydroartemisinin']
