UNASSIGNED: Context Hypericum perforatum L. (Hypericaceae), used in moderate @entity308 treatment, is active in experimental tests for antidepressant activity. For H. connatum Lam., a South American species lacking hyperforin, antidepressant effects have not been demonstrated. Objective This study evaluates the antidepressant-like effect of H. connatum in @entity35 and identifies the components involved in this activity. Materials and methods First, the effects of acute and 14-d oral administrations of an extract derived from H. connatum aerial parts were studied using the Escape Deficit (ED) test. Next, @entity1042 -extracted @entity3096 -enriched fractions B and C and fraction-purified @entity3088 ( @entity3092 , @entity15604 and @entity63982 ) were evaluated in the ED test after acute administration. To rule out possible confounding effects of the @entity3088 , we examined nociceptive threshold using the tail-flick test and anxious behaviour using the elevated plus maze (EPM) test. Results Hypericum connatum increased reactivity of unavoidable stress-exposed @entity35 after acute (0.5 and 1   g/kg: ED   =   18.6/30 and 19.8/30, respectively) and repeated administration (0.5   g/kg twice daily: ED   =   17.8/30). Protective effects were observed for fractions B and C (250   mg/kg: ED   =   18.1/30 and 18.8/30, respectively), @entity3092 (2.5, 5 and 10   mg/kg: ED   =   15.3/30, 18.3/30 and 21.6/30, respectively), @entity15604 (5 and 10   mg/kg: ED   =   15.4/30 and 13.0/30, respectively) and @entity63982 (2.5   mg/kg: ED   =   19.2/30). The @entity3088 did not modify nociceptive threshold or performance in the EPM test. Discussion and conclusion Hypericum connatum showed protective activity in the ED test, a correlate of potential antidepressant-like effects that appeared to be related to the @entity3096 components of this species.

The protective effect of Hypericum connatum on stress-induced escape deficit in XXXX is related to its @entity3096 content.

@entity308 :: ('MESH:D003866', 'Disease') :: ['depression'] @entity1042 :: ('MESH:D000432', 'Chemical') :: ['methanol'] @entity15604 :: ('MESH:D012431', 'Chemical') :: ['rutin'] @entity63982 :: ('MESH:C016527', 'Chemical') :: ['isoquercitrin'] @entity3088 :: ('MESH:D005419', 'Chemical') :: ['flavonoids'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity3092 :: ('MESH:D011794', 'Chemical') :: ['quercetin'] @entity3096 :: ('CHEBI:47916', 'Chemical') :: ['flavonoid']
@entity35 :: (10116,Species) :: ['rat']
