OBJECTIVE: To study the effect of @entity18042 on the adherence of leukemic @entity2280 + KG1a cell to @entity1 umbilical vein endothelial cell line ECV-304 cell and on the expression of adhesive molecules. METHODS: Adhesion assay and adhesion blocking assay were respectively applied to measure the effect of @entity18042 and/or adhesion molecule monoclonal antibodies on the adhesion property of KG1a. The expressions of adhesion molecules were determined by RT-PCR and FACS analysis. RESULTS: The adhesion of KG1a cells to ECV-304 was significantly enhanced in the presence of @entity18042 . Such enhancement was also observed when KG1a or ECV-304 cells were separately treated with @entity18042 before interaction. The adhesion capacity of KG1a cells was reduced when cells were co-incubated with the blocking monoclonal antibodies (MoAbs) against @entity5690 , @entity4725 , @entity2071 , respectively. In contrast, MoAbs against @entity1196 , @entity8729 and @entity4105 had no such effect. During a period of 3 hours when KG1a or ECV-304 cells were respectively incubated with @entity18042 , the mRNA expressions of @entity5690 , @entity2071 were up-regulated. Furthermore, when KG1a or ECV-304 cells were incubated with @entity18042 for 2 hours, respectively, the percentages of @entity5690 + KG1a cells and @entity2071 + ECV-304 were increased significantly. CONCLUSION: @entity18042 can enhance KG1a cell adhesive capacity by increasing the expressions of adhesion molecules.

[Effect of platelet factor 4 on the adhesive property of leukemic XXXX + KG1a cell].

@entity1 :: ('9606', 'Species') :: ['human'] @entity18042 :: ('5196', 'Gene') :: ['PF4'] @entity5690 :: ('3676', 'Gene') :: ['CD49 d', 'CD49d'] @entity1196 :: ('6402', 'Gene') :: ['CD62L'] @entity4105 :: ('6401', 'Gene') :: ['CD62E'] @entity8729 :: ('6403', 'Gene') :: ['CD62P'] @entity4725 :: ('7412', 'Gene') :: ['CD106'] @entity2280 :: ('947', 'Gene') :: ['CD34'] @entity2071 :: ('3383', 'Gene') :: ['CD54']
@entity2280 :: (947,Gene) :: ['CD34']
