AIM: Increased age is the most prominent risk factor for the initiation and progression of @entity571 ( @entity571 ). The effects of @entity1 @entity2124 (hGH) combined or not with hyaluronan @entity6346 derivative (HAD) were evaluated on @entity1 @entity571 chondrocytes, to define their biological action and potentiality in @entity571 treatment. MATERIAL AND METHODS: Cell viability, metabolic activity, gene expression and factors released were tested at different time points on chondrocytes treated with different concentrations of hGH (0.01-10    g/ml) alone or in combination with HAD (1   mg/ml). RESULTS: We found that @entity571 chondrocytes express @entity9045 and that the different doses of hGH tested did not affect cell viability, metabolic activity or the expression of collagen type 2, 1, or 10 nor did it induce the release of @entity442 or @entity540 . Conversely, hGH treatment increased the expression of hyaluronan receptor @entity1759 . HAD combined with hGH reduced metabolic activity, @entity650 release and gene expression, but not the @entity20139 ( @entity20139 ), which was significantly induced and translocated into the nucleus. The parameters analyzed, independently of the treatments used proportionally decreased with increasing age of the @entity1 . CONCLUSIONS: hGH only induced @entity1759 receptor on @entity571 chondrocytes but did not affect other parameters, such as chondrocytic gene markers or @entity442 or @entity540 release. HAD reduced all the effects induced by hGH partially through a significant induction of @entity20139 . These data show that GH or HAD treatment does not influence the response of the @entity571 chondrocytes, thus the modulation of cellular response is age-independent.

Age-independent effects of hyaluronan @entity6346 derivative and XXXX on @entity1 osteoarthritic chondrocytes.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity650 :: ('3569', 'Gene') :: ['IL6'] @entity20139 :: ('8835', 'Gene') :: ['suppressor of cytokine signaling 2', 'SOCS2'] @entity571 :: ('MESH:D010003', 'Disease') :: ['osteoarthritis', 'OA'] @entity442 :: ('3479', 'Gene') :: ['IGF-1'] @entity1759 :: ('960', 'Gene') :: ['CD44'] @entity2124 :: ('2688', 'Gene') :: ['growth hormone'] @entity540 :: ('2247', 'Gene') :: ['FGF-2'] @entity6346 :: ('CHEBI:32988', 'Chemical') :: ['amide'] @entity9045 :: ('2690', 'Gene') :: ['GH receptor']
@entity2124 :: (2688,Gene) :: ['growth hormone']
