BACKGROUND: @entity1 with @entity1082 )-positive @entity957 ( @entity957 ) have a better prognosis than those with @entity5 . There is interest in de-escalating their treatment but strategies are needed for risk stratification to identify subsets with a poor prognosis. This study investigated @entity5 -infiltrating lymphocytes (TILs) in relation to @entity5 status and @entity1 survival. METHODS: Biopsies from 218 @entity1 diagnosed with @entity957 between 2002 and 2011, who underwent chemo/radiotherapy were analysed for HPV by PCR, in-situ hybridisation and @entity986 immunohistochemistry (IHC). One hundred and thirty-nine samples with concordant HPV detection were analysed for CD3, @entity404 , @entity405 and @entity403 expression in @entity5 and stromal regions using multiplexIHC and multispectral image analysis. Labelling of @entity3960 ( @entity3960 ) identified activated stroma. RESULTS: @entity1082 -positive compared with HPV-negative @entity957 had higher infiltration in both @entity5 and stromal areas of @entity404 and @entity405 T cells but not @entity403 T regulatory cells. Only CD3+ @entity405 + stromal and not @entity5 area infiltration was associated with increased survival (P=0.02). There was significantly higher @entity3960 expression in HPV-positive compared with -negative @entity5 , which did not correlate with survival. CONCLUSIONS: Studies of TILs for risk stratification in @entity957 should assess stromal infiltration.

Stromal infiltration of @entity405 T cells is associated with improved clinical outcome in XXXX .

@entity1 :: ('9606', 'Species') :: ['Patients', 'patients', 'patient'] @entity404 :: ('920', 'Gene') :: ['CD4'] @entity986 :: ('1029', 'Gene') :: ['p16'] @entity5 :: ('MESH:D009369', 'Disease') :: ['HPV-negative tumours', 'HPV tumour', 'tumours', 'tumour'] @entity957 :: ('MESH:D002294', 'Disease') :: ['OPSCC', 'oropharyngeal squamous cell carcinoma'] @entity3960 :: ('MESH:D018235', 'Disease') :: ['SMA', 'smooth muscle actin'] @entity1082 :: ('10566', 'Species') :: ['Human papillomavirus', 'human papillomavirus (HPV'] @entity403 :: ('50943', 'Gene') :: ['FoxP3'] @entity405 :: ('925', 'Gene') :: ['CD8']
@entity957 :: (MESH:D002294,Disease) :: ['HPV-positive oropharyngeal squamous carcinoma']
