STUDY OBJECTIVES: Abnormal remodeling of the extracellular matrix (ECM) has been implicated in the pathogenesis of @entity2107 . However, the contribution of lung parenchymal cells to ECM remodeling after mechanical injury is not well defined. The objective of these studies was to investigate in vitro the release of @entity22534 and their respective inhibitors @entity69941 , and to explore potential regulation by @entity1618 . DESIGN: @entity19 fetal epithelial cells and fibroblasts isolated on E18-19 of gestation were exposed to 20% cyclic stretch to simulate @entity651 . @entity5780 and @entity1235 activity were investigated by zymography and ELISA. @entity5779 and @entity5783 abundance were analyzed by Western blot. RESULTS: We found that mechanical stretch increased @entity5780 and decreased @entity5783 in fibroblasts, indicating that excessive stretch promotes @entity5780 activation, expressed as the @entity5780 / @entity5783 ratio. Incubation with @entity1618 did not change @entity5780 activity. In contrast, mechanical stretch of epithelial cells decreased @entity1235 activity and the @entity1235 / @entity5779 ratio by 60-70%. When @entity1618 was added, mechanical stretch increased the @entity1235 / @entity5779 ratio by 50%. CONCLUSIONS: We conclude that mechanical stretch differentially affects @entity22534 and their inhibitors in fetal lung cells. @entity1618 modulates @entity1235 activity through a combination of effects on @entity1235 and @entity5779 levels.

Differential expression of XXXX and their inhibitors in fetal lung cells exposed to mechanical stretch: regulation by @entity1618 .

@entity5783 :: ('21858(Tax:10090)', 'Gene') :: ['TIMP-2'] @entity69941 :: ('21858;21857', 'Gene') :: ['TIMP-2 and -1'] @entity19 :: ('10090', 'Species') :: ['Mouse'] @entity5780 :: ('17390(Tax:10090)', 'Gene') :: ['MMP-2'] @entity5779 :: ('21857(Tax:10090)', 'Gene') :: ['TIMP-1'] @entity1618 :: ('16153(Tax:10090)', 'Gene') :: ['IL-10'] @entity651 :: ('MESH:D055370', 'Disease') :: ['lung injury'] @entity2107 :: ('MESH:D001997', 'Disease') :: ['bronchopulmonary dysplasia'] @entity1235 :: ('17395(Tax:10090)', 'Gene') :: ['MMP-9'] @entity22534 :: ('17390;17395', 'Gene') :: ['MMP-2 and -9', 'MMP-2/9']
@entity22534 :: (17390;17395,Gene) :: ['MMP-2 and -9']
