INTRODUCTION: Compounds exhibiting antioxidant activity have received much interest in the food industry because of their potential health benefits. Carotenoids such as @entity14319 , which in the @entity1 diet mainly derives from tomatoes ( @entity523 ), have attracted much attention in this aspect and the study of their extraction, processing and storage procedures is of importance. Optical techniques potentially offer advantageous non-invasive and specific methods to monitor them. OBJECTIVES: To obtain both fluorescence and Raman information to ascertain if ultrasound assisted extraction from @entity523 pulp has a detrimental effect on @entity14319 . METHOD: Use of time-resolved fluorescence spectroscopy to monitor carotenoids in a @entity4315 extract obtained from @entity523 pulp with application of ultrasound treatment (583 kHz). The resultant spectra were a combination of scattering and fluorescence. Because of their different timescales, decay associated spectra could be used to separate fluorescence and Raman information. This simultaneous acquisition of two complementary techniques was coupled with a very high time-resolution fluorescence lifetime measurement of the @entity14319 . RESULTS: Spectroscopic data showed the presence of @entity36428 and @entity38 in addition to @entity14319 in the @entity523 extract. The time-resolved spectral measurement containing both fluorescence and Raman data, coupled with high resolution time-resolved measurements, where a lifetime of ~5 ps was attributed to @entity14319 , indicated @entity14319 appeared unaltered by ultrasound treatment. Detrimental changes were, however, observed in both @entity38 and @entity36428 contributions. CONCLUSION: Extracted @entity14319 appeared unaffected by ultrasound treatment, while other constituents ( @entity38 and @entity36428 ) were degraded.

Use of time-resolved spectroscopy as a method to monitor carotenoids present in XXXX extract obtained using ultrasound treatment.

@entity1 :: ('9606', 'Species') :: ['human'] @entity36428 :: ('MESH:C003140', 'Chemical') :: ['phytofluene'] @entity4315 :: ('CHEBI:29021', 'Chemical') :: ['hexane'] @entity523 :: ('4081', 'Species') :: ['tomato', 'Solanum lycopersicum'] @entity14319 :: ('MESH:C015329', 'Chemical') :: ['lycopene'] @entity38 :: ('MESH:D002734', 'Chemical') :: ['chlorophyll']
@entity523 :: (4081,Species) :: ['tomato']
