The long-term objective of this study is to elucidate the role of bladder mucosal glycosaminoglycans and @entity3558 glycoproteins in the development of @entity2335 and other @entity1494 . Bladder biopsies and urine samples from @entity1 and healthy controls were analyzed for glycoconjugates by biochemical and immunochemical methods. Due to the limited availability of @entity1 bladders for research purposes, detailed analysis of @entity95 bladders glycoconjugates were also carried out. Biochemical analysis of @entity95 bladders indicate that while the major portion of the glycoconjugates in the urothelium is sialoglycoprotein, low levels of @entity31847 and chondroitin @entity597 are also present. The correlating immunohistochemical data show very weak staining of the @entity95 bladder epithelium by antiglycosaminoglycan antibodies. In contrast, the lamina propria and muscle layers stained intensely for @entity12006 and hyaluronic acid. Thus, the quantity of glycosaminoglycans associated with the bladder epithelial layer, particularly as extracellular matrix components on the luminal surface of the bladder, appears insignificant. On the other hand, several lectins and anti-epitectin (a @entity70100 sialoglycoprotein) antibodies showed strong staining of the luminal surface of @entity95 and normal @entity1 bladders. Further, preliminary results with anti-epitectin antibodies reveal a weaker and patchy staining of biopsy specimens from @entity2335 @entity1 compared to controls. The urinary levels of glycosaminoglycans and epitectin, in @entity2335 @entity1 and healthy controls were determined by chemical or immunoassays. Urinary epitectin, but not glycosaminoglycans, was decreased in @entity2335 @entity1 .

An investigation of the nature of bladder mucosal glycoconjugates and their role in XXXX .

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity1494 :: ('MESH:D001745', 'Disease') :: ['bladder diseases'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity31847 :: ('MESH:D006497', 'Chemical') :: ['heparan sulfate'] @entity12006 :: ('MESH:D002809', 'Chemical') :: ['chondroitin sulfate'] @entity70100 :: ('100009240(Tax:9986)', 'Gene') :: ['MUC1'] @entity3558 :: ('100508689', 'Gene') :: ['mucin'] @entity2335 :: ('MESH:D003556', 'Disease') :: ['interstitial cystitis'] @entity597 :: ('CHEBI:16189', 'Chemical') :: ['sulfate']
@entity2335 :: (MESH:D003556,Disease) :: ['interstitial cystitis']
