We have designed this method for the comprehensive and adequate analysis of neurotransmitters and metabolites including @entity934 ( @entity1617 ) and @entity1877 in specific brain nuclei. In physiological and pharmacological studies, the role of specific neurotransmitters in the central nervous system (CNS) has often been studied by microinjection of their agonist and antagonist into the target regions, with the action and role of the transmitter in question being deduced in a relatively straightforward manner from the results. However, none of these studies have investigated fluctuation in neurotransmitter and metabolite contents in response to a variety of stimuli in vivo. No comprehensive analysis of neurotransmitters in small specific area in the CNS has been undertaken due to the difficulties of proper analysis of @entity1617 and @entity1877 . Different types of neuronal systems in the CNS affect each other. Thus, it is not necessarily clear that different types of neurons do in fact respond to an administered agonist and antagonist. For example, dopaminergic neuron mediated control of cholinergic interneuron in the striatum and dopaminergic regulation of cortical @entity1617 release have been documented. Similarly, the cholinergic system affects dopaminergic and noradrenergic neurons. Intraventricular administration of an @entity1617 esterase inhibitor, @entity1339 , increases @entity935 and @entity1063 release in the hypothalamus. So, an adequate comprehensive analysis of the transmitters and the metabolites in the same tissue sample provides valuable aid to clarify what types of neurons truly respond to the administered drugs and the excitation of physiological events. For an appropriate analysis we employed a microwave device, which made the estimation of @entity1617 and @entity1877 possible. In addition to this, we employed a punch technique for the microdissection of the specific nuclei and area from the brain slice.

Comprehensive analysis of neurotransmitters and their metabolites including @entity934 and XXXX in rat brain nuclei.

@entity935 :: ('MESH:D004298', 'Chemical') :: ['dopamine'] @entity1617 :: ('CHEBI:22614', 'Chemical') :: ['ACh'] @entity1877 :: ('MESH:D002794', 'Chemical') :: ['choline'] @entity1063 :: ('MESH:D009638', 'Chemical') :: ['noradrenaline'] @entity1339 :: ('MESH:D009388', 'Chemical') :: ['neostigmine'] @entity934 :: ('MESH:D000109', 'Chemical') :: ['acetylcholine']
@entity1877 :: (MESH:D002794,Chemical) :: ['choline']
