Molecular size and net charge of isoforms of pathogenesis-related (PR) chitinase, @entity69406 and @entity53705 were studied in uninfected @entity2247 ( @entity2247 ., v. Karat) leaves and in @entity2247 leaves infected with the pathogenic fungus Drechslera teres f. teres (Sacch.) Shoem. Molecular characteristics were determined by time-dependent @entity2975 gradient gel electrophoresis under native conditions and by applying an extended version of the computer program MOL-MASS (Rothe, G. M., Weidmann, H., Electrophoresis 1991, 12, 703-709). Uninfected @entity2247 leaves contained predominantly one @entity53705 isozyme but also three very weak peroxidases. Activities of all of these three peroxidases increased considerably after infection with Drechslera teres. The molecular masses of peroxidases 1 and 3 were estimated to be 38 +/- 5 and 42 +/- 7 kDa and their apparent valences at pH 8.4 were Z = 3.13 and 3.20, respectively. Amongst the chitinase isoforms, chitinase 1 and chitinase 2 appeared after infection, while chitinase 3 was also observed in uninfected leaves of @entity2247 . The molecular mass of chitinase 3 (31 +/- 6 kDa; f/fo = 1.20) was larger than that of chitinase 1 (20 +/- 2 kDa; f/fo = 1.04) and chitinase 2 (23 +/- 3 kDa; f/fo = 1.06). The valence of constitutive chitinase 3 (Z = 1.44 +/- 0.81) at pH 8.4 was lower than that of adaptive chitinase 1 (Z = 3.27 +/- 1.02) and chitinase 2 (Z = 2.96 +/- 1.38). Infection of @entity2247 leaves with Drechslera teres also induced the hydrolytic enzyme @entity69406 1; @entity69406 2 appeared in uninfected and in infected leaves. Constitutive @entity69406 2 was smaller (molecular mass 19 +/- kDa; f/fo = 1.05) than adaptive @entity69406 1 (molecular mass 26 +/- 4 kDa; f/fo = 1.07). The valence of adaptive @entity69406 1 (Z = 9.58 +/- 4.17) was approximately threefold that of @entity69406 2 (Z = 2.80 +/- 0.93).

Molecular size and net charge of pathogenesis-related enzymes from XXXX ( @entity2247 ., v. Karat) infected with Drechslera teres f. teres (Sacch.) Shoem.

@entity69406 :: ('100101495(Tax:4513)', 'Gene') :: ['beta-1,3-glucanase'] @entity53705 :: ('548137(Tax:4513)', 'Gene') :: ['peroxidase'] @entity2247 :: ('4513', 'Species') :: ['barley', 'Hordeum vulgare L'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide']
@entity2247 :: (4513,Species) :: ['barley']
