This study examines proliferative activity in @entity5 cells of @entity1 with histologically documented invasive @entity0 treated with magnetic resonance-guided interstitial laser photocoagulation (MR-GILP). Immunohistochemical marker for @entity1364 ( @entity1364 ), a nuclear protein abundant in actively proliferating cells, is used. The study demonstrates the effectiveness of MR-GILP in ablating @entity5 cells of infiltrating @entity0 . The diagnosis of infiltrating @entity0 was confirmed by core needle biopsies. Using a specially designed magnetic resonance imaging (MRI) device, rotating delivery of excitation off-resonance (RODEO), @entity5 were measured ranging from 1.8 to 4.0 cm in greatest dimension. Seven @entity406 -fixed, @entity982 -embedded archival tissues from seven @entity1 with infiltrating @entity189 , status post-MR-GILP, were analyzed. Using @entity1364 immunoperoxidase (Biomeda Corp.), the proliferative capability of the remaining @entity5 cells around the focus of laser photocoagulation was determined. The lesions were digitally acquired using a Nikon Eclipse E800 microscope with an automated stage. Images were analyzed using Cool SNAP image editing software (version 1.0). Appropriate thresholds were set for positive staining and limited concentric radial measurements of equal area between all samples were compared at radial millimeter intervals from the center of laser ablation. The integrated area occupied by @entity1364 -positive cells per radial millimeter from the charcoal site (the center of the laser) increased as the distance from this site increased (a mean average at each radial measurement revealed: at the 1 mm radius the positive integrated area was 0.0024 mm2; at 2 mm, 0.0145 mm2; at 3 mm, 0.0351 mm2; at 4 mm, 0.0696 mm2; at 5 mm, 0.1025 mm2; and at 6 mm, 0.1263 mm2). MR-GILP is an effective mean of ablating @entity0 . This treatment option may represent an alternative to lumpectomy for a single lesion < or =1 cm, or make @entity1 with two separate lesions eligible for lumpectomy.

Assessment of @entity1364 activity using digital image analysis in XXXX following magnetic resonance-guided interstitial laser photocoagulation.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity1364 :: ('5111', 'Gene') :: ['proliferating cell nuclear antigen', 'PCNA'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast carcinoma', 'breast cancer'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity406 :: ('MESH:D005557', 'Chemical') :: ['formalin'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinoma'] @entity982 :: ('MESH:D010232', 'Chemical') :: ['paraffin']
@entity0 :: (MESH:D001943,Disease) :: ['breast carcinoma']
