The alteration of @entity876 and @entity984 gene in 36 @entity2 of workers occupationally exposed to @entity3440 (LCWS) was studied. DNA was extracted from paraffin\ embedded tissues and amplified by PCR. PCR-SS-CP analysis on exons 5, 7 and 8 of @entity876 gene revealed that 43.8% of mutations were clustered in exon 8 of @entity876 gene. In @entity2 , the mutation rate of exon 8 was fluctuated around 20%. The prevalence of mutations was the highest (70%) in @entity151 ( @entity151 ) and the lowest in @entity420 (33%). In our study the mutation rate was high in @entity420 (53.9%) and low in @entity151 (30.8%). The @entity984 gene of LCWS also revealed a different mutation pattern. Unexpectedly, no mutation was found at codon 12 in 36 LCWS by PCR-RFLP, which is the predominant mutation in @entity984 gene in @entity2 . The point mutation study by DNA sequencing confirmed the absence of codon 12 mutation in @entity984 gene in LCWS. The mutations observed in this study were frequently distributed in codons 13 and 15, principally a G-C transversion, instead of G-T transversion in @entity2 .

[Evidence on the carcinogenicity of XXXX at DNA level in human].

@entity984 :: ('3845', 'Gene') :: ['K-ras'] @entity2 :: ('MESH:D008175', 'Disease') :: ['ordinary lung cancer', 'lung cancers'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity3440 :: ('MESH:D012822', 'Chemical') :: ['silica'] @entity151 :: ('MESH:D055752', 'Disease') :: ['SCLC', 'small cell lung cancer'] @entity420 :: ('MESH:D000230', 'Disease') :: ['adenocarcinoma']
@entity3440 :: (MESH:D012822,Chemical) :: ['silica']
