Binding of phenyl derivative of pyranocoumarin (PDP) modulated activity of fungal endopolygalacturonase in silico. Induced fit docking study of PDP with endopolygalacturonase (1HG8) showed a bifurcated @entity3884 bond interaction with the protein at Lys 244 with a docking score of -3.6 and glide energy of -37.30  kcal/mol. Docking with endopolygalacturonase II (1CZF) resulted @entity3884 bond formation with Lys 258 with a docking score of -2.3 and glide energy of -30.42  kcal/mol. It was hypothesized that this modulation favors accumulation of cell wall fragments (oligogalacturonides) which act as elicitors of plant defense responses. In order to prove the same, in vivo studies were carried out using a formulation developed from PDP (PDP 5EC) on greenhouse grown @entity523 L. The formulation was effective at different concentrations in reduction of seed @entity281 , improvement of vigor and control of @entity4278 @entity10327 . lycopersici @entity281 in @entity523 . At a concentration of 2  %, PDP 5EC significant reduction in seed @entity281 (95.83  %), improvement in seed vigor (64.31  %) and control of @entity4278 @entity10327 . lycopersici @entity281 (96.15  %) were observed. Further application of PDP 5EC to @entity523 challenged with @entity4278 @entity10327 . lycopersici significantly increased the activity of enzymes of the phenylpropanoid pathway, namely, peroxidase (PO), @entity3978 oxidase (PPO), @entity815 ammonia lyase (PAL), and enhanced the total phenolic content when compared to the control.

Phenyl Derivative of Pyranocoumarin Precludes XXXX @entity10327 . lycopersici Infection in @entity523 via Induction of Enzymes of the Phenylpropanoid Pathway.

@entity3978 :: ('CHEBI:26195', 'Chemical') :: ['polyphenol'] @entity10327 :: ('239', 'Species') :: ['f.sp'] @entity3884 :: ('MESH:D006859', 'Chemical') :: ['hydrogen'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity4278 :: ('5507', 'Species') :: ['Fusarium oxysporum', 'F. oxysporum'] @entity523 :: ('4081', 'Species') :: ['L. esculentum', 'Lycopersicon esculentum'] @entity815 :: ('CHEBI:28044', 'Chemical') :: ['phenylalanine']
@entity4278 :: (5507,Species) :: ['Fusarium oxysporum']
