BACKGROUND: One of the potential effects of IL-12 is to restore @entity1198 /Th2 balance. Therefore, we investigated the possibility of developing a system for local delivery of IL-12 into the airways by examining protein expression in a @entity1 bronchial epithelial cell line (BEAS-2B) after adenoviral IL-12 gene transduction. The effects of @entity619 on the gene-modified cells were also examined. METHODS: Adenoviral vectors AxCAegfp and Ax1CIhp40ip35 were used to transduce enhanced green fluorescence protein and IL-12 genes, respectively, into BEAS-2B cells. Wild-type and IL-12 gene-transduced BEAS-2B cells were then incubated with or without @entity619 , and concentrations of IL-12, @entity379 , @entity650 , @entity687 , @entity2841 and chemokines ( @entity14420 and @entity5737 ) in the supernatant were measured by ELISA. IL-12 receptor expression was analysed by flow cytometry and RT-PCR. RESULTS: The efficiency of transgene expression in BEAS-2B cells at a multiplicity of infection of 30 was approximately 80%. Gene-modified BEAS-2B cells produced biologically active IL-12, regardless of @entity619 treatment. While IL-12 gene transduction led to increased production of @entity650 and @entity687 by BEAS-2B cells, expressions of these proteins were suppressed by @entity619 . Addition of exogenous IL-12 failed to augment BEAS-2B cell @entity650 and @entity687 production, and IL-12 receptor expression by BEAS-2B cells was not detected. CONCLUSIONS: Our findings suggest that adenoviral IL-12 gene transduction may be effective in inducing IL-12 expression in the airways, and could be a potential approach in the management of @entity565 .

Adenoviral interleukin-12 gene transduction into XXXX bronchial epithelial cells: up-regulation of pro-inflammatory cytokines and its prevention by corticosteroids.

@entity1 :: ('9606', 'Species') :: ['human'] @entity650 :: ('3569', 'Gene') :: ['IL-6'] @entity5737 :: ('6352', 'Gene') :: ['RANTES'] @entity565 :: ('MESH:D001249', 'Disease') :: ['bronchial asthma'] @entity1198 :: ('51497', 'Gene') :: ['Th1'] @entity14420 :: ('6361', 'Gene') :: ['TARC'] @entity619 :: ('MESH:D003907', 'Chemical') :: ['dexamethasone'] @entity379 :: ('3458', 'Gene') :: ['IFN-gamma'] @entity687 :: ('3576', 'Gene') :: ['IL-8'] @entity2841 :: ('1437', 'Gene') :: ['granulocyte macrophage-colony stimulating factor']
@entity1 :: (9606,Species) :: ['human']
