Mesangial cells are thought to promote glomerular macrophage accumulation in @entity631 . This may occur through the production of @entity23346 ( @entity19984 ), a molecule known to regulate macrophage accumulation at sites of @entity32 . To study this, glomerular @entity19984 expression and macrophage accumulation were examined in rat anti-Thy-1 disease, a model of @entity1310 . In situ hybridization and immunohistochemistry showed that @entity19984 is expressed by some podocytes in normal @entity35 glomeruli. De novo @entity19984 expression by glomerular endothelium was seen on day 1 of anti-Thy-1 disease. On day 6, glomerular @entity19984 mRNA and protein expression were prominent in @entity1347 , which was also the location of most infiltrating macrophages. Double-staining identified de novo @entity19984 mRNA and protein expression by proliferating mesangial cells within these lesions. Cytokine regulation of mesangial cell @entity19984 expression was examined in vitro. Northern blotting showed that cultured @entity35 mesangial cells express a single 0.6-kb species of @entity19984 mRNA, and Western blotting detected a single protein band of 12.5 kD. Six-hour stimulation of mesangial cells with @entity3692 or platelet-derived growth factor significantly increased @entity19984 mRNA levels. However, the addition of recombinant @entity19984 to mesangial cells did not affect mesangial cell proliferation or constitutive transforming growth factor-beta mRNA expression, nor did @entity19984 induce @entity657 mRNA expression. In conclusion, this is the first study to demonstrate that mesangial cells can produce @entity19984 in vivo and in vitro. It is postulated that mesangial cell @entity19984 production in response to injury acts to promote macrophage accumulation within @entity1347 in rat anti-Thy-1 @entity1310 .

@entity35 mesangial cells express XXXX in vitro and in vivo.

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity1310 :: ('MESH:D009393', 'Disease') :: ['nephritis', 'mesangioproliferative nephritis'] @entity3692 :: ('25712(Tax:10116)', 'Gene') :: ['interferon-gamma'] @entity657 :: ('24770(Tax:10116)', 'Gene') :: ['monocyte chemoattractant protein-1'] @entity1347 :: ('MESH:C537775', 'Disease') :: ['segmental proliferative lesions'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity631 :: ('MESH:D005921', 'Disease') :: ['glomerulonephritis'] @entity19984 :: ('81683(Tax:10116)', 'Gene') :: ['MIF'] @entity23346 :: ('103694877', 'Gene') :: ['macrophage migration inhibitory factor']
@entity23346 :: (103694877,Gene) :: ['macrophage migration inhibitory factor']
