UNASSIGNED: Surface modification of materials designed for regenerative medicine may improve biocompatibility and functionality. The application of glycosaminoglycans (GAGs) and chemically sulphated GAG derivatives is a promising approach for designing functional biomaterials, since GAGs interact with cell-derived growth factors and have been shown to support fibroblast growth in two-dimensional (2D) cultures. Here, coatings with artificial extracellular matrix (aECM), consisting of the structural protein collagen I and the GAG hyaluronan (HA) or sulphated HA derivatives, were investigated for their applicability in a three-dimensional (3D) system. As a model, macroporous @entity7050 ( @entity7050 ) scaffolds were homogeneously coated with aECM. The resulting scaffolds were characterized by compressive moduli of 0.9-1.2   MPa and pore sizes of 40-420     m. @entity1 dermal fibroblasts (dFbs) colonized these aECM-coated @entity7050 scaffolds to a depth of 400     m within 14   days. In aECM-coated scaffolds, collagen I(a1) and collagen III(a1) mRNA expression was reduced, while @entity3334 ( @entity3334 ) mRNA expression was increased within 7   days, suggesting matrix-degradation processes. Stimulation with @entity2533 generally increased cell density and collagen synthesis, demonstrating the efficiency of bioactive molecules in this 3D model. Thus, aECM with sulphated HA may modulate the effectivity of @entity2533 -induced collagen I(a1) expression, as demonstrated previously in 2D systems. Overall, the tested aECM with modified HA is also a suitable material for fibroblast growth under 3D conditions. Copyright    2015 John Wiley _ Sons, Ltd.

Artificial extracellular matrices support cell growth and matrix synthesis of XXXX dermal fibroblasts in macroporous 3D scaffolds.

@entity1 :: ('9606', 'Species') :: ['Human'] @entity7050 :: ('MESH:C043435', 'Chemical') :: ['PLGA', 'poly(lactic-co-glycolic acid)'] @entity2533 :: ('7040', 'Gene') :: ['TGFb1'] @entity3334 :: ('4312', 'Gene') :: ['MMP-1', 'matrix metalloproteinase-1']
@entity1 :: (9606,Species) :: ['human']
