BACKGROUND: @entity10926 ( @entity6507 ) is caused mainly by @entity3307 ) with an annual incidence of 1-4 cases/million inhabitants. Currently, @entity6507 treatment faces difficulties such as the use of antivirals with @entity137 , metabolic side effects and @entity3307 resistance. An alternative to antivirals is the use of small interfering RNA (siRNA) as a viral replication inhibitor. In this work, siRNA targeting the UL-39 region was evaluated for @entity6507 treatment in vivo. METHODS: BALB/c @entity19 were inoculated with @entity3307 and treated with siRNA. The treatment was evaluated through kinetics of @entity3307 replication inhibition, number of siRNA doses administered and treatment with siRNA plus @entity1033 . All groups were evaluated for signs of @entity6507 , mortality and @entity3307 replication inhibition. RESULTS: The treated group of the kinetic experiment demonstrated a reduction of @entity6507 signs and an @entity3307 replication inhibition of 43.6-99.9% in the brain and 53-98% in @entity21 ( @entity21 ). Animals treated with one or two doses of siRNA had a prolonged survival time, reduced clinical signs of @entity6507 and @entity3307 replication inhibition of 67.7% in brains and 85.7% in @entity21 of animals treated with two doses of siRNA. Also, animals treated with siRNA plus @entity1033 demonstrated reduced signs of @entity6507 and mortality, as well as @entity3307 replication inhibition in the brain (83.2%) and @entity21 (74.5%). CONCLUSIONS: These findings demonstrated that siRNA was capable of reducing @entity6507 clinical signs, prolonging survival time and inhibiting @entity3307 replication in @entity19 . Thus, siRNA can be a potential alternative to the standard @entity6507 treatment especially to reduce clinical signs and extend survival time in vivo.

Effects of RNA interference therapy against XXXX encephalitis.

@entity1033 :: ('MESH:D000212', 'Chemical') :: ['acyclovir'] @entity137 :: ('MESH:D064420', 'Disease') :: ['elevated toxicity'] @entity6507 :: ('8630', 'Gene') :: ['HSE'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity21 :: ('MESH:D001480', 'Disease') :: ['TG', 'trigeminal ganglia'] @entity10926 :: ('MESH:D020803', 'Disease') :: ['Herpetic encephalitis'] @entity3307 :: ('10298', 'Species') :: ['herpes simplex virus type 1 (HSV-1', 'HSV-1']
@entity3307 :: (10298,Species) :: ['herpes simplex virus type 1']
