PURPOSE: Cancers have developed a number of strategies to escape immune responses including the differential expression of costimulatory molecules of the B7 family. @entity49045 have recently been described in different @entity5 entities but the relevance for @entity320 has not yet been studied so far. EXPERIMENTAL DESIGN: Using immunohistochemistry, @entity49045 expression was studied on 29 @entity320 . Survival curves and log-rank tests were used to test the association of protein expression with survival. Cell lines were evaluated for @entity49045 expression by PCR and flow cytometry. Functional T-cell- @entity5 coculture assays were carried out with in vitro generated @entity5 transfectants. RESULTS: @entity49045 expression was detected in @entity5 lesions (29 of 29 and 28 of 29) and in metastases (28 of 29 and 26 of 29). The numbers of @entity469 (+) macrophages were significantly lower in @entity1 with low @entity32261 expression, whereas CD8(+) T-cell infiltrates were independent of expression levels. Furthermore, a survival benefit for @entity1 with @entity32261 low expressing @entity320 was found, whereas @entity25032 was not associated with any clinical parameter. All 23 @entity320 cell lines analyzed expressed @entity49045 mRNA and protein, but @entity32261 was restricted to intracellular compartments. On silencing of @entity25032 by specific shRNA @entity5 -associated antigen-specific T cell responses were unaltered. Overexpression of @entity32261 on @entity320 cells did not alter the cytotoxicity of different CD8(+) effector cells, but drastically inhibited cytokine production. CONCLUSIONS: Our study provides for the first time evidence of @entity32261 expression on @entity320 cells as a mechanism controlling @entity5 immunity which is associated with @entity1 ' survival.

@entity32261 expression in @entity1 XXXX : its association with @entity1 ' survival and antitumor immune response.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity49045 :: ('80381;79679', 'Gene') :: ['B7-H3 and B7-H4'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'primary tumor'] @entity469 :: ('968', 'Gene') :: ['CD68'] @entity32261 :: ('79679', 'Gene') :: ['B7-H4'] @entity25032 :: ('80381', 'Gene') :: ['B7-H3'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma', 'melanoma lesions']
@entity320 :: (MESH:D008545,Disease) :: ['melanoma']
