MicroRNAs (miRNAs) are a type of small non-coding RNAs that are often play important roles in @entity105 , but the carcinogenic mechanism of miRNAs is still unclear. This study will investigate the function and the mechanism of @entity39758 in @entity189 (GC). The expression of @entity39758 in GC and the DNA copy number of @entity39758 were detected by real-time PCR. The effect of @entity39758 on cell proliferation was measured by counting kit-8 assay. Different assays, including bioinformatics algorithms (TargetScan and miRanda), luciferase report assay and Western blotting, were used to identify the target gene of @entity39758 in GC. The expression of @entity39758 target gene in clinical CRC tissues was also validated by immunohistochemical assay. From this research, we found that @entity39758 was downregulated in GC tissues compared with corresponding noncancerous tissues (NCTs), and the DNA copy number of @entity39758 was lower in GC than NCTs, which may induce the corresponding downregulation of @entity39758 in GC. Ectopic expression of @entity39758 inhibited GC cell growth in vitro. Subsequently, we identified that @entity9293 is the target gene of @entity39758 in GC, and silencing @entity9293 expression phenocopied the inhibitory effect of @entity39758 on GC cell proliferation. Furthermore, we observed that @entity9293 was overexpressed in GC tissues, and high expression of @entity9293 in GC predicted poor overall survival. In summary, we revealed that @entity39758 functions as a @entity5 suppressor in GC through inhibiting @entity9293 .

MicroRNA-638 inhibits cell proliferation by targeting XXXX in human gastric carcinoma.

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinoma'] @entity9293 :: ('5337', 'Gene') :: ['PLD1'] @entity39758 :: ('693223', 'Gene') :: ['miR-638'] @entity105 :: ('MESH:D063646', 'Disease') :: ['carcinogenesis']
@entity9293 :: (5337,Gene) :: ['phospholipase D1']
