BACKGROUND: Determination of methylated @entity29410 ( @entity68701 ) in plasma has been shown to be a sensitive and specific biomarker for @entity14 ( @entity14 ). However, the relationship between methylated DNA in plasma and colon tissue of the same subjects has not been reported. METHODS: Plasma and matching biopsy samples were collected from 24 @entity1 with no evidence of disease (NED), 26 @entity1 with @entity652 and 34 @entity1 with @entity14 . Following @entity9736 conversion of DNA a commercial RT-PCR assay was used to determine the total amount of DNA in each sample and the fraction of @entity68701 DNA. The @entity29410 protein was assessed using immunohistochemistry. RESULTS: The percent of methylated reference (PMR) values for @entity29410 above a PMR threshold of 1% were detected in 4.2% (1/24) of NED, 100% (26/26) of @entity652 and 97.1% (33/34) of @entity14 tissues. PMR differences between NED vs. @entity652 and NED vs. @entity14 comparisons were significant (p<0.001). In matching plasma samples using a PMR cut-off level of 0.01%, @entity29410 methylation was 8.3% (2/24) of NED, 30.8% (8/26) of @entity652 and 88.2% (30/34) of @entity14 . Significant PMR differences were observed between NED vs. @entity14 (p<0.01) and @entity652 vs. @entity14 (p<0.01). Significant differences (p<0.01) were found in the amount of cfDNA (circulating cell-free DNA) between NED and @entity14 , and a modest correlation was observed between @entity68701 concentration and cfDNA of @entity5 (R2=0.48). The level of @entity29410 protein in tissues was inversely correlated to @entity68701 levels with abundant expression in normals, and diminished expression in @entity652 and @entity5 . CONCLUSIONS: Methylated @entity29410 was detected in all tissue samples. In plasma samples, elevated @entity68701 values were detected in @entity14 , but not in @entity652 . Tissue levels of @entity68701 alone are not sufficient to predict @entity68701 levels in plasma. Additional parameters including the amount of cfDNA in plasma appear to also play a role.

Detection of methylated XXXX in tissue and plasma of colorectal @entity1 with @entity5 and the relationship to the amount of circulating cell-free DNA.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer', 'tumors'] @entity9736 :: ('MESH:C042345', 'Chemical') :: ['bisulfite'] @entity14 :: ('MESH:D015179', 'Disease') :: ['CRC', 'colorectal cancer'] @entity29410 :: ('10801', 'Gene') :: ['Septin 9', 'SEPT9', 'Septin-9'] @entity652 :: ('MESH:D000236', 'Disease') :: ['adenomas', 'adenoma'] @entity68701 :: ('53860(Tax:10090)', 'Gene') :: ['mSEPT9']
@entity29410 :: (10801,Gene) :: ['septin 9']
