Objective To construct the lentivirus carrying the mutated palmitoylation site of the linker for activation of T cells ( @entity6070 ) and infect Jurkat cells with it to establish stable cell line, and to investigate the effect of @entity6070 palmitoylation mutation on T cell signaling induced by @entity1009 . Methods Negative control (neg-EGFP) and @entity6070 -M-EGFP fusion protein gene vectors were respectively constructed and then packaged using lentivirus. Subsequently, Jurkat cells were infected with them to establish stable cell lines. Confocal laser scanning microscopy was used to observe the @entity281 efficiency and the distribution of fusion proteins in Jurkat cells. CCK-8 assay was used to detect the change of cell proliferation activity after @entity1009 mAb supplementation. Flow cytometry was used to determine the apoptosis rate. Western blotting was used to examine the levels of phospholipase C-y1 (PLC-y1) and lymphocyte-specific protein @entity4149 kinase ( @entity2952 ). Results Confocal laser scanning microscopy revealed that @entity6070 molecules of @entity6070 -M group scattered on cell membrane, and there was no obvious clustered region after cross linkage with @entity1009 mAb. Compared with the negative control group, the cell proliferation activity of @entity6070 -M group significantly decreased, and the quantity of middle-late apoptotic cells significantly increased; Western blotting showed that the expression levels of PLC-y1 and @entity2952 in @entity6070 -M group was roughly the same with those in negative control group, and after @entity1009 mAb stimulation, there was no obvious change in @entity6070 -M group, while the levels in negative control group were reduced. Conclusion @entity6070 -M-EGFP fusion protein could not locate on lipid rafts of Jurkat cells infected with @entity6070 palmitoylation mutation. In addition, the growth of the cells carrying the @entity6070 -M-EGFP was inhibited. The palmitoylation mutation of @entity6070 attenuated the signal transduction induced by @entity4495 -anchored @entity1009 in T cells.

[Mutation of palmitoylation site of linker for activation of T cells inhibits signal transduction mediated by @entity4495 -anchored XXXX in T cells].

@entity6070 :: ('27040', 'Gene') :: ['LAT'] @entity1009 :: ('966', 'Gene') :: ['CD59'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity2952 :: ('3932', 'Gene') :: ['LCK'] @entity4495 :: ('MESH:D017261', 'Chemical') :: ['glycosylphosphatidylinositol'] @entity4149 :: ('D014443', 'Chemical') :: ['tyrosine']
@entity1009 :: (966,Gene) :: ['CD59']
