RATIONALE: The time required for the effective clearance of @entity456 /organization after intrapleural fibrinolytic therapy (IPFT) is unknown. Chest ultrasonography and computed tomography (CT) were used to assess the efficacy of IPFT in a @entity95 model of @entity803 -induced @entity130 , treated with single chain (sc) urokinase (scuPA) or tissue (sctPA) plasminogen activators. METHODS: IPFT with sctPA (0.145mg/kg; n=10) and scuPA (0.5mg/kg; n=12) was monitored by serial ultrasonography alone (n=12) or alongside CT scanning (n=10). IPFT efficacy was assessed with Gross @entity651 (GLIS) and Ultrasonography ( @entity2106 ) Scores. Pleural fluids withdrawn at 0-240min and 24h after IPFT were assayed for plasminogen activating (PA) and fibrinolytic activities, a-macroglobulin/fibrinolysin-complexes, active plasminogen activator inhibitor 1 (PAI-1). RESULTS: scuPA and sctPA generated comparable steady-state fibrinolytic activities by 20min. PA activity in the scuPA group decreased slower than the sctPA group (kobs=0.016 and 0.042min(-1)). Significant amounts of bioactive @entity3730 /a-macroglobulin (but not tPA; P<0.05) complexes accumulated at 0-40min after IPFT. Despite the differences in intrapleural processing, IPFT with either fibrinolysin was effective (GLIS <=10) in animals imaged with ultrasonography only. @entity2106 correlated well with postmortem GLIS (r(2)=0.85) and confirmed relatively slow intrapleural fibrinolysis after IPFT, which coincided with effective clearance of adhesions/organization at 4-8h. CT-scanning was associated with less effective (GLIS>10) IPFT and higher levels of active PAI-1 at 24h following therapy. CONCLUSION: Intrapleural fibrinolysis in @entity803 -induced @entity130 in @entity95 is relatively slow (4-8h). In CT-scanned animals, elevated PAI-1 activity (possibly radiation-induced) reduced the efficacy of IPFT, buttressing the major impact of active PAI-1 on IPFT outcomes.

The time course of resolution of adhesions during fibrinolyitic therapy in @entity803 -induced XXXX in @entity95 .

@entity3730 :: ('100008633(Tax:9986)', 'Gene') :: ['uPA'] @entity95 :: ('9986', 'Species') :: ['rabbits', 'rabbit'] @entity651 :: ('MESH:D055370', 'Disease') :: ['Lung Injury'] @entity803 :: ('MESH:D013752', 'Chemical') :: ['tetracycline'] @entity2106 :: ('MESH:D011697', 'Disease') :: ['USS'] @entity130 :: ('MESH:D014947', 'Disease') :: ['pleural injury'] @entity456 :: ('MESH:D010995', 'Disease') :: ['pleural adhesions']
@entity130 :: (MESH:D014947,Disease) :: ['pleural injury']
