OBJECTIVE: To assay antifilaggrin autoantibodies, we developed an enzyme-linked immunosorbent assay (ELISA) using a "citrullinated" recombinant @entity35 filaggrin. Our objectives were to assess its value for diagnosing @entity309 ( @entity309 ) and to compare the results with those obtained using 4 other reference methods for detection of antifilaggrin autoantibodies, including the commercially available ELISA that uses a modified "citrullinated" synthetic peptide derived from the sequence of @entity1 filaggrin (CCP-ELISA). METHODS: We analyzed 711 sera from @entity1 with well-characterized @entity627 , including 240 @entity1 with @entity309 . Antifilaggrin autoantibodies were detected by an ELISA using a recombinant @entity35 filaggrin deiminated in vitro as immunosorbent (ArFA-ELISA). The results considered were the differences between the optical densities obtained on deiminated and nondeiminated proteins. Antibodies to @entity35 esophagus epithelium were detected by indirect immunofluorescence, while antibodies to @entity1 filaggrin were detected by immunoblotting and by a recently described ELISA using a deiminated recombinant @entity1 filaggrin. Finally, CCP-ELISA was performed according to the manufacturer's recommendations. RESULTS: At the titer thresholds allowing diagnostic specificities of 0.95, 0.985, and 0.99 to be reached, the diagnostic sensitivities of the ArFA-ELISA were 0.76, 0.67, and 0.65, respectively. At these 3 thresholds, the sensitivities were significantly higher than those of the 4 other tests. Despite incomplete overlapping of the 5 tests, the high diagnostic performance of the ArFA-ELISA allows us to propose this test to replace all the other methods for antifilaggrin autoantibody detection. CONCLUSION: ArFA-ELISA appears to be the most efficient test among those available for the detection of antifilaggrin autoantibodies, in terms of diagnostic accuracy for @entity309 . Its diagnostic performance in early @entity309 and its prognostic value are currently under evaluation.

Detection of antibodies to deiminated recombinant XXXX filaggrin by enzyme-linked immunosorbent assay: a highly effective test for the diagnosis of @entity309 .

@entity35 :: ('10116', 'Species') :: ['rat'] @entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity627 :: ('MESH:D012216', 'Disease') :: ['rheumatic diseases'] @entity309 :: ('MESH:D001172', 'Disease') :: ['rheumatoid arthritis', 'RA']
@entity35 :: (10116,Species) :: ['rat']
