BACKGROUND: The contribution of @entity842 (NO) to immune response and matrix degradation in the periodontal environment suggests a role for NO and @entity3623 ( @entity3623 ) activity in the pathogenesis of @entity205 (CsA)-induced @entity8032 ( @entity24481 ). However, current knowledge on this topic is limited to experimental animal studies. The present study was undertaken on the basis of a hypothesis whether altered @entity3542 / @entity1507 levels in gingival crevicular fluid (GCF) and @entity9554 (eNOS) and @entity3623 ( @entity3623 ) immunoreactivity in gingiva of CsA-treated @entity1 contribute to the pathogenesis of CsA-induced @entity24481 . METHODS: Twenty-four CsA-medicated renal transplant @entity1 with @entity24481 ( @entity24481 +; n = 12) or without @entity24481 ( @entity24481 -; n = 12), 10 @entity2522 , and 10 healthy subjects were included in the study. GCF samples from two proximal sites facing interdental papilla were collected, and papilla was excised. @entity3623 and eNOS were determined by immunohistochemistry. GCF @entity3542 / @entity1507 levels were analyzed based on the Griess reaction. RESULTS: Weak @entity3623 immunostaining was observed in the healthy and @entity24481 - groups. In the @entity2522 and @entity24481 + groups, @entity3623 immunostaining significantly increased in connective tissue. Epithelial immunostaining of @entity3623 was localized to basal keratinocytes and the lower layer of stratum (str.) spinosum in the @entity2522 group. In the @entity24481 + group, @entity3623 immunostaining was differentially localized to keratinocytes of str. superficiale but considerably decreased in the str. basale. Weak eNOS immunostaining was found in the healthy and @entity24481 - groups, whereas higher immunostaining was observed in the @entity2522 and @entity24481 + groups. No intergroup differences were observed regarding @entity3542 / @entity1507 levels in GCF. CONCLUSION: CsA differentially upregulated @entity3623 , but not eNOS, in overgrown gingiva, which may play a pivotal role in the pathogenesis of CsA-induced @entity24481 .

Immunohistochemical analysis of inducible and endothelial forms of @entity842 synthase in XXXX -induced @entity8032 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity9554 :: ('4846', 'Gene') :: ['endothelial NOS'] @entity2522 :: ('MESH:D005891', 'Disease') :: ['gingivitis'] @entity3542 :: ('CHEBI:16301', 'Chemical') :: ['nitrite'] @entity1507 :: ('CHEBI:48107', 'Chemical') :: ['nitrate'] @entity24481 :: ('MESH:C537799', 'Disease') :: ['GO'] @entity205 :: ('MESH:D016572', 'Chemical') :: ['cyclosporin A'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity3623 :: ('4843', 'Gene') :: ['inducible NOS', 'NO-synthase', 'NOS', 'iNOS'] @entity8032 :: ('MESH:D019214', 'Disease') :: ['gingival overgrowth']
@entity205 :: (MESH:D016572,Chemical) :: ['cyclosporin A']
