BACKGROUND: @entity262 ( @entity262 ) is a diagnosis made using clinical, laboratory, and histologic parameters. Aberrant @entity3138 and/or @entity2902 expression on mast cells provides one minor criterion for a diagnosis of @entity262 . To validate a tube ( @entity1195 / @entity2467 / @entity3138 / @entity2902 ) for mast cell evaluation, we performed flow cytometry (FC) on residual material from marrow aspirates samples submitted to the hematopathology laboratory. METHODS: Samples evaluated (n=98) had no clinical or morphologic suspicion for @entity262 . Samples were excluded if there was history of a myeloid stem cell @entity5 . Ten documented cases of @entity262 were evaluated for comparison. RESULTS: Among cases without history of @entity262 , 17.3% (n=17) showed expression of @entity3138 and/or @entity2902 on >= 10% of the mast cell population ( @entity2902 alone in 14 cases, @entity3138 alone in 2 cases, both in one case), while 82.6% (n=81) showed no expression of these antigens. The percentage of mast cells showing aberrant @entity3138 and/or @entity2902 expression respectively ranged from 12.1%-98.8% and 22.2%-95.7% Interestingly, all of the cases with evidence of aberrant antigen expression on mast cells were collected post-therapy while 22.1% of the negative samples were collected pre-therapy. A cut-off of 60% @entity2902 expression on mast cells identified all cases of @entity262 while minimizing false positives. CONCLUSIONS: These findings demonstrate that aberrant expression of @entity3138 and/or @entity2902 may be seen on mast cells outside of the setting of @entity262 . The data suggests that this phenomenon may be seen more commonly following chemotherapy and that FC of mast cells should be interpreted with caution in the post-chemotherapy setting. This article is protected by copyright. All rights reserved.

Expression of @entity3138 and XXXX on mast cell populations can be seen outside the setting of systemic mastocytosis.

@entity5 :: ('MESH:D009369', 'Disease') :: ['neoplasm'] @entity1195 :: ('5788', 'Gene') :: ['CD45'] @entity262 :: ('MESH:D034721', 'Disease') :: ['SM', 'Systemic mastocytosis'] @entity3138 :: ('914', 'Gene') :: ['CD2'] @entity2467 :: ('3815', 'Gene') :: ['CD117'] @entity2902 :: ('3559', 'Gene') :: ['CD25']
@entity2902 :: (3559,Gene) :: ['CD25']
