BACKGROUND: Osteopontin is a macrophage adhesive protein that is expressed by renal tubules in @entity1986 . METHODS: To investigate the function of @entity1189 , we induced @entity1986 in @entity1189 null mutant ( @entity1189 -/-) and wild-type ( @entity1189 +/+) @entity19 by unilateral ureteral ligation. Tissue was analyzed for macrophages (ED-1), types I and IV collagen deposition, @entity641 expression, and for tubular and interstitial cell apoptosis. RESULTS: Obstructed kidneys from both @entity1189 -/- and @entity1189 +/+ @entity19 developed @entity3041 , @entity194 , @entity32 and @entity82 . @entity1189 was absent in @entity1189 -/- kidneys but was increased in obstructed @entity1189 +/+ kidneys. Macrophage influx, measured by computer-assisted quantitative immunostaining, was less in @entity1189 -/- @entity19 compared to @entity1189 +/+ @entity19 at day 4 (threefold, P < 0.02), day 7 (fivefold, P < 0.02), but not at day 14. Interstitial deposition of types I and IV collagen were also two- to fourfold less in obstructed @entity1189 -/- kidneys (P < 0.02). There was also a reduction of @entity641 mRNA expression in the interstitium at day 7 (by in situ hybridization) and a near significant 34% reduction in cortical @entity641 activity (P = 0.06) compared to obstructed @entity1189 +/+ kidneys at day 14. Obstructed kidneys from @entity1189 -/- @entity19 also had more interstitial and tubular apoptotic cells (TUNEL assay) compared to obstructed @entity1189 +/+ @entity19 at all time points. The ability of @entity1189 to act as a cell survival factor was also documented by showing that the apoptosis of serum-starved NRK52E renal epithelial cells was markedly enhanced in the presence of neutralizing anti- @entity1189 antibody. CONCLUSION: @entity1189 mediates early interstitial macrophage influx and interstitial @entity82 in unilateral @entity1695 . @entity1189 may also function as a survival factor for renal tubulointerstitial cells.

Obstructive uropathy in the @entity19 : role of osteopontin in interstitial XXXX and apoptosis.

@entity32 :: ('MESH:D007249', 'Disease') :: ['interstitial inflammation'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity641 :: ('21803(Tax:10090)', 'Gene') :: ['TGF-beta'] @entity1695 :: ('MESH:D014517', 'Disease') :: ['ureteral obstruction'] @entity3041 :: ('MESH:D006869', 'Disease') :: ['hydronephrosis'] @entity1189 :: ('20750(Tax:10090)', 'Gene') :: ['OPN'] @entity194 :: ('MESH:D001284', 'Disease') :: ['tubular atrophy'] @entity1986 :: ('MESH:D009395', 'Disease') :: ['tubulointerstitial disease']
@entity82 :: (MESH:D005355,Disease) :: ['fibrosis']
