BACKGROUND: @entity689 (NA) is an essential surface protein for @entity683 virus replication. NA inhibitors are commonly used for the treatment of @entity683 @entity1 in Japan. Several mutations that reduce the effect of NA inhibitors have been reported. We sequenced the whole NA segment of isolated virus from @entity683 @entity1 and investigated the relation between the NA @entity3689 sequence and the 50% inhibitory concentration (IC50) of four NA inhibitors. MATERIALS AND METHODS: A total of 20 viruses that showed high or low IC50 of NA inhibitors were selected from A/H1N1pdm09, A/H3N2, and B isolates from the viruses isolated from @entity1 in the 2013-14 @entity683 season. Viral RNA was extracted and RT-PCR was done. The amplified genome was sequenced using a next generation sequencer", and the deduced @entity3689 sequences were analyzed. RESULTS: Two A/H1N1pdm09 viruses that showed very high IC50 for @entity684 (150 nM and 130 nM) contained the @entity15998 mutation. Otherwise, no significant relation was found between the NA @entity3534 and the IC50 of the four NA inhibitors. There was no significant relation between the NA @entity3534 and the IC50 of the four NA inhibitors for A/H3N2 viruses. The B viruses that showed a high IC50 for @entity684 and @entity24677 shared some @entity3534 . The B viruses that showed a high IC50 of @entity685 and @entity23217 also shared some @entity3534 . They were different from the shared @entity3534 found for @entity684 and @entity24677 . CONCLUSION: The previously reported @entity15998 mutation that causes @entity684 resistance was found in the two A/H1N1pdm09 viruses that showed a very high IC50 for @entity684 . No additional NA @entity3689 sequences related to the IC50 of the four NA inhibitors was found. The meaning of the shared @entity3534 among B viruses that showed a high IC50 would be an interesting target for further investigation.

[Analysis of the XXXX @entity3689 Sequences of Influenza A/H1N1pdm09, A/H3N2, and B Viruses Isolated from Influenza @entity1 in the 2013/14 Japanese Influenza Season].

@entity689 :: ('4758', 'Gene') :: ['Neuraminidase'] @entity1 :: ('9606', 'Species') :: ['patients'] @entity3534 :: ('MESH:D000596', 'Chemical') :: ['amino acids'] @entity684 :: ('MESH:D053139', 'Chemical') :: ['oseltamivir'] @entity685 :: ('MESH:D053243', 'Chemical') :: ['zanamivir'] @entity683 :: ('MESH:D007251', 'Disease') :: ['influenza'] @entity15998 :: ('p|SUB|H|275|Y', 'ProteinMutation') :: ['H275Y'] @entity24677 :: ('MESH:C546918', 'Chemical') :: ['laninamivir'] @entity23217 :: ('MESH:C414210', 'Chemical') :: ['peramivir'] @entity3689 :: ('D000596', 'Chemical') :: ['amino acid']
@entity689 :: (4758,Gene) :: ['Neuraminidase']
