@entity3064 is an important opportunistic and multidrug-resistant pathogen leading to @entity580 . Over the last 10 years, a significant and threatening increase in resistance to carbapenems, mainly due to the dissemination of class D beta-lactamases, has been reported in A.baumannii worldwide. The most common types of beta-lactamases causing @entity2893 resistance in A.baumannii are the @entity15799 , OXA-24, OXA-40, OXA-58 and OXA-143 type @entity563 beta-lactamases. The aim of this study was to investigate the presence of OXA type beta-lactamases in @entity2893 -resistant A.baumannii strains and the clonal relationship between the strains. A total of 105 non-duplicate @entity2893 -resistant A.baumannii strains isolated from various clinical samples (68 blood, 18 bronchoalveolar lavage, 13 drainage, 3 urine, 2 cerebrospinal fluid and 1 catheter samples) in the Microbiology Laboratories of Selcuk University, Meram (2009-2012) and Selcuklu (2007-2008) Medical School Hospitals, were included in the study. The isolates were identified by conventional methods and Phoenix 100 BD (BD Diagnostic, USA) and Vitek II (bioMerieux, France) automated systems. @entity2893 susceptibility test was performed by Kirby-Bauer disk diffusion method according to the CLSI standards. bla(OXA 23-like), bla(OXA 24-like), bla(OXA 58-like) and bla(OXA 51-like) genes were amplified by multiplex PCR assay and clonal relatedness was investigated by pulsed-field gel electrophoresis (PFGE) using ApaI enzyme. The bla(OXA 51-like) gene was determined in all @entity2893 -resistant A.baumannii isolates, while the bla(OXA 23-like) and bla(OXA 58-like) genes were detected in 46.6% and 53.3% of isolates, respectively. However bla(OXA 24-like) gene was not demonstrated in any isolates. bla(OXA 23-like) gene was determined in both Meram and Selcuklu Medical School hospitals, but bla(OXA 58-like) gene was detected only in Meram Medical School hospital. PFGE analysis of the isolates revealed 32 different groups in bla(OXA 23-like) producing A.baumannii strains and 23 different groups determined in bla(OXA 58-like) producing strains. No common epidemic isolates were detected in the two hospitals, however it was noted that some clones produced small outbreaks in Meram MS hospital. In this study it was shown that bla(OXA 23-like) and bla(OXA 58-like) genes together with bla(OXA 51-like) gene had significant roles in the @entity2893 -resistance of A.baumannii strains. @entity2893 -resistant A.baumannii strains producing bla(OXA 23-like) and bla(OXA 58-like) enzymes showed the epidemic potential of this nosocomial pathogen and the requirement of molecular typing methods to identify the epidemiologic relationship of the isolates.

[Investigation of OXA type beta-lactamases and PFGE patterns in XXXX strains resistant to carbapenems].

@entity15799 :: ('20472025', 'Gene') :: ['OXA-23'] @entity2893 :: ('CHEBI:46765', 'Chemical') :: ['carbapenem', 'Carbapenem'] @entity563 :: ('CHEBI:17822', 'Chemical') :: ['serine'] @entity3064 :: ('470', 'Species') :: ['Acinetobacter baumannii'] @entity580 :: ('MESH:D003428', 'Disease') :: ['nosocomial infections']
@entity3064 :: (470,Species) :: ['Acinetobacter baumannii']
