@entity95 ovarian preovulatory follicles and in particular the overlying surface epithelium were studied by morphological and ultrahistochemical means at different times after mating. By light microscopy an increase of cytoplasmic granules was found in the surface epithelium at the follicle apex 4 h after mating. The granules increased in amount and showed maximal accumulation 8-9 h after mating. They then disappeared at the same time as the connective tissue elements in the underlying tunica albuginea and theca externa disintegrated. Transmission electron microscopy showed that the membrane-bounded granules or dense bodies fused with one another and by 8 h after mating they often changed character and appeared more electron lucent. Furthermore, open communications were found between altered granules and vacuoles and between vacuoles and the extracellular space below the epithelium. Acid phosphatase reaction product was localized to the granules and Golgi cisternae. Not all the dense bodies were enzyme positive. At later stages, close to the time of follicle @entity397 , the epithelial cells were attenuated and thin, with only a few granules. By scanning electron microscopy it was found that the epithelial cells at the follicle apex increased in size approaching the time of follicle @entity397 and that their microvilli decreased in number and in size. At 8 h and later, the contours of intracellular granules could be visualized. The results of this study were similar to those found when @entity95 were induced to ovulate by HCG-stimulation. This further strengthens the hypothesis that the surface epithelium contributes porteolytic enzymes which help to disintegrate the follicle apex prior to @entity397 .

Structural alterations of XXXX ovarian follicles after mating with special reference to the overlying surface epithelium.

@entity397 :: ('MESH:D012421', 'Disease') :: ['rupture'] @entity95 :: ('9986', 'Species') :: ['Rabbit', 'rabbits']
@entity95 :: (9986,Species) :: ['rabbit']
