OBJECTIVE: @entity413 is an important substrate for energy production in the developing heart. Increased @entity413 uptake rate and metabolism during @entity63 and reperfusion are closely linked to postischemic myocardial recovery. The initial rate-limiting step for glycolysis is the transport of @entity413 across the plasma membrane by @entity413 transporters ( @entity54129 and @entity54128 ). We hypothesized that changes in @entity54129 and @entity54128 expression in developing hearts lead to age-dependent adaptive changes in @entity413 uptake capacity and influence tolerance to @entity63 . METHODS: Western-immunoblotting was performed to determine @entity54129 and @entity54128 expression in myocardial tissue from 1, 2, and 3-week-old and adult @entity95 . @entity413 uptake rate was measured with @entity9715 -nuclear magnetic resonance spectroscopy using @entity897 as substrate in isolated perfused hearts. Hearts from same age @entity95 were perfused in the Langendorff mode with crystalloid buffer or buffer plus a @entity54128 specific antibody in order to determine @entity54128 mediated effects on myocardial protection. The hearts were subjected to 30 minutes of normothermic @entity63 followed by reperfusion. Cardiac contractile function measurements were obtained pre- and postischemia. Tissue @entity880 accumulation was measured in all groups at end- @entity63 CONCLUSIONS: Insulin-regulated @entity413 transporter ( @entity54128 ) expression in the heart increased gradually after birth reaching nearly adult levels by 3 weeks of age. Corresponding with the higher amount of @entity54128 protein, improved recovery of postischemic contractile function was seen in older hearts in association with increased anaerobic glycolytic capacity. Interventions to accelerate postnatal @entity54128 expression may improve ischemic tolerance in the neonatal heart.

Postnatal increase in insulin-sensitive XXXX transporter expression is associated with improved recovery of postischemic myocardial function.

@entity54128 :: ('100037710(Tax:9986)', 'Gene') :: ['GLUT-4'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemia'] @entity9715 :: ('CHEBI:37971', 'Chemical') :: ['(31)P'] @entity95 :: ('9986', 'Species') :: ['rabbits'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity54129 :: ('100125988(Tax:9986)', 'Gene') :: ['GLUT-1'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose', 'Glucose'] @entity897 :: ('MESH:D003847', 'Chemical') :: ['2-deoxyglucose']
@entity413 :: (MESH:D005947,Chemical) :: ['glucose']
