BACKGROUND: @entity364 is a major cause of @entity1939 . To understand the mechanism of both innate and adaptive immunity in @entity281 is of great significance in the treatment and prevention of @entity364 . Our previous study concerned innate immunity. Here, we explored the potential role of @entity24373 in adaptive immunity of @entity364 . METHODS: @entity1 corneal epithelial cells (HCECs) were stimulated with @entity54 hyphae (10(6) pieces/ml) with or without @entity24373 siRNA, and Peripheral blood mononuclear cells (PBMCs) were cultured with or without @entity24373 . HCECs and PBMCs were co-cultured in a transwell system for various periods. Then we collected PBMCs and detected the proliferation and activation as well as T helper type 2 (Th2) differentiation by flow cytometry and quantitative RT-PCR. IgG and IgA levels in supernatants of PBMCs were measured by means of ELISA. RESULTS: @entity24373 could induce a Th2 response in vitro, and the expression of @entity24373 was highly increased in HCECs stimulated with A. fumigatus hyphae. @entity54 -infected HCECs were capable of promoting @entity1 lymphocyte proliferation, and activating @entity1 @entity404 (+) T cells, @entity405 (+) T cells, and B cells by up-regulating expression of activation marker @entity7986 . Importantly, Th2 differentiation of @entity404 (+) T cells was induced during co-culture with A. fumigatus-infected HCECs in a transwell system. Intersetingly, blockade of @entity24373 using siRNA prevented the proliferation and activation of lymphocytes as well as Th2 differentiation. We also detected an increased IgG level that was associated with @entity24373 . CONCLUSION: These findings suggested that HCEC-derived @entity24373 has a key role in adaptive immune responses of @entity364 via skewing Th2 differentiation and promoting humoral immunity. This article is protected by copyright. All rights reserved.

XXXX promotes T helper type 2 responses through thymic stromal lymphopoietin production by @entity1 corneal epithelial cells.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity404 :: ('920', 'Gene') :: ['CD4'] @entity1939 :: ('MESH:D001766', 'Disease') :: ['blindness'] @entity281 :: ('MESH:D007239', 'Disease') :: ['corneal infection'] @entity364 :: ('MESH:D009181', 'Disease') :: ['Fungal keratitis', 'fungal keratitis'] @entity54 :: ('746128', 'Species') :: ['Aspergillus fumigatus'] @entity405 :: ('925', 'Gene') :: ['CD8'] @entity7986 :: ('969', 'Gene') :: ['CD69'] @entity24373 :: ('85480', 'Gene') :: ['TSLP']
@entity54 :: (746128,Species) :: ['Aspergillus fumigatus']
