UNASSIGNED: Recently, our laboratory has demonstrated the technical feasibility of monitoring @entity935 at 1 min temporal resolution with microdialysis and online liquid chromatography. Here, we monitor @entity935 in the @entity35 striatum during local delivery of high @entity972 /low @entity1372 or @entity7831 in awake-behaving @entity35 . Microdialysis probes were implanted and perfused continuously with or without @entity619 in the perfusion fluid for 4 days. @entity619 is an anti-inflammatory agent that exhibits several positive effects on the apparent health of the brain tissue surrounding microdialysis probes. @entity935 was monitored 1 or 4 days after implantation under basal conditions, during 10 min applications of 60 mM or 100 mM @entity3529 , and during 15 min applications of 10  M @entity7831 . High @entity3529 and @entity7831 were delivered locally by adding them to the microdialysis perfusion fluid using a computer-controlled, low-dead-volume six-port valve. Each day/ @entity3529 / @entity619 combination elicited specific @entity935 responses. @entity619 treatment increased @entity935 levels in basal dialysates (i.e., in the absence of @entity3529 or @entity7831 ). Applications of 60 mM @entity3529 evoked distinct responses on days one and four after probe implantation, depending upon the presence or absence of @entity619 , consistent with @entity619 's ability to mitigate the traumatic effect of probe implantation. Applications of 100 mM @entity3529 evoked dramatic oscillations in @entity935 levels that correlated with changes in the field potential at a metal electrode implanted adjacent to the microdialysis probe. This combination of results indicates the role of spreading depolarization in response to 100 mM @entity3529 . With 1 min temporal resolution, we find that it is possible to characterize the pharmacokinetics of the response to the local delivery of @entity7831 . Overall, the findings reported here confirm the benefits arising from the ability to monitor @entity935 via microdialysis at high sensitivity and at high temporal resolution.

Monitoring @entity935 Responses to XXXX Ion and @entity7831 by in Vivo Microdialysis with Online Liquid Chromatography at One-Minute Resolution.

@entity7831 :: ('MESH:D009627', 'Chemical') :: ['nomifensine'] @entity3529 :: ('D011188', 'Chemical') :: ['K(+)'] @entity935 :: ('MESH:D004298', 'Chemical') :: ['dopamine', 'Dopamine'] @entity1372 :: ('MESH:D012964', 'Chemical') :: ['sodium'] @entity619 :: ('MESH:D003907', 'Chemical') :: ['Dexamethasone', 'dexamethasone'] @entity972 :: ('MESH:D011188', 'Chemical') :: ['potassium'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats']
@entity972 :: (MESH:D011188,Chemical) :: ['Potassium']
