Intravascular large @entity1892 (IVLBL) is a rare @entity5 characterized by proliferation of @entity706 cells within the blood vessels. The cell origin of IVLBL has not yet been determined. We examined cell lineage, with immunohistochemical staining and molecular analysis, using polymerase chain reaction (PCR) of the variable region of the @entity7639 (Ig-VH) gene. We also investigated the cell origin using direct sequence analysis of the complementary-determining region 2 (CDR2) and framework region 3 (FR3) in six cases, consisting of two male and four female @entity1 . The sequences in five cases showed frequent mutations. The percent homology to their closest germline genes ranged from 74.7 to 91.8%. However, one case showed a percent homology of 99.4% in CDR2 and FR3 of Ig-VH. All cases showed rearrangements of VH3 family genes. Interestingly, three of the IVLBL cases with hypermutated IgH genes showed the expression of @entity5893 . Therefore, expression of @entity5893 in @entity706 cells does not indicate that the origin of IVLBL is the same as @entity1073 having the character of @entity5893 expression, which develops in pre-germinal center cells. Our results indicate that most IVLBLs may originate in the post-germinal center cells, based on the presence of somatic mutation in VH genes, although some heterogeneous cases are intermingled within IVLBL.

Analysis of the @entity7639 gene variable region of intravascular large XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity706 :: ('MESH:D008223', 'Disease') :: ['lymphoma'] @entity5 :: ('MESH:D009369', 'Disease') :: ['neoplasm'] @entity5893 :: ('921', 'Gene') :: ['CD5'] @entity1892 :: ('MESH:D016393', 'Disease') :: ['B-cell lymphoma'] @entity1073 :: ('MESH:D020522', 'Disease') :: ['mantle cell lymphoma'] @entity7639 :: ('3495', 'Gene') :: ['immunoglobulin heavy chain']
@entity1892 :: (MESH:D016393,Disease) :: ['B-cell lymphoma']
