BACKGROUND: Classification of molar gestation into @entity1475 ( @entity1475 ) and @entity1475 ( @entity1475 ) is done according to clinical, ultrasonographic, histologic and genetic criteria. However, making a distinction between @entity1475 and @entity1475 using histologic criteria alone may be difficult and several studies have shown that misclassifications are frequent, even for experienced pathologists. @entity1475 is the most common precursor to @entity1466 and heterozygous moles carry an increased predisposition to transformation. METHODS: Formalin-fixed, @entity982 -embedded tissue sections of @entity1 as well as peripheral blood of @entity1 and their partners' were collected in EDTA tubes. Tissue samples were obtained by curettage. Histological evaluation was performed on routine section stained with @entity1710 and @entity1711 . Variable Number Tandem Repeats (VNTRs) genotyping was performed for 30 cases in two groups of @entity1475 (n=21) and @entity1475 (n=9), with Polymerase Chain Reaction (PCR) amplification of 2 different polymorphic loci, namely the @entity794 and D1S80. RESULTS: The results of DNA analysis by VNTR genotyping showed that in 16 cases of @entity1475 , amplification of the VNTR polymorphic loci showed androgenetic mono-spermic moles (homozygote) and in 5 cases of @entity1475 androgenetic dispermic moles (heterozygote) in molar tissue. In cases of @entity1475 , 6 samples were triploid dispermic and 3 samples were diploid biparental. CONCLUSION: This study confirmed that VNTR genotyping can identify the parental source of polymorphic alleles in hydatidiform mole. Compared to STR genotyping, VNTR genotyping was performed by PCR amplification of several minisatellite markers of DNA. This method significantly requires less time and is cost-effective.

Variable Number Tandem Repeat (VNTR) Genotyping of Hydatidiform Mole in Iranian XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity1466 :: ('MESH:D002822', 'Disease') :: ['choriocarcinoma'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['Eosin'] @entity794 :: ('1280', 'Gene') :: ['Col2A1'] @entity982 :: ('MESH:D010232', 'Chemical') :: ['paraffin'] @entity1475 :: ('MESH:D006828', 'Disease') :: ['Partial Hydatidiform Mole', 'CHM', 'PHM', 'Complete Hydatidiform Mole'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['Hematoxylin']
@entity1 :: (9606,Species) :: ['Patients']
