OBJECTIVE: This study was undertaken to determine the effect of @entity842 (NO) on tissue factor (TF) expression in vascular smooth muscle cells. STUDY DESIGN: @entity35 aortic smooth muscle cells (RASMCs) were exposed to NO delivered exogenously with the NO donor @entity16475 (SNAP) or produced endogenously after infection with an adenoviral vector carrying @entity1 @entity3623 (AdiNOS). Functional TF activity was assessed with chromogenic TF assay. TF antigen was determined with immunohistochemistry. Northern blot analysis was used to determine steady- state TF messenger RNA (mRNA). Electrophoretic mobility gel shift assay was performed to determine the nuclear binding activity of @entity2451 ( @entity2451 ). @entity2451 activity was inhibited by either prior transduction of RASMCs with mutant IkappaB or treatment with @entity13373 . RESULTS: RASMCs exposed to SNAP or infected with AdiNOS exhibited increased functional TF activity and antigen. Regardless of the source of NO, a time-dependent and concentration-dependent increase in TF activity was observed. Steady-state TF mRNA levels were also increased by NO delivered via either method. @entity2451 nuclear binding activity was also increased by NO. Inhibition of @entity2451 activity by either @entity13373 treatment or mutant IkappaB transduction abrogated NO-induced enhancement of TF mRNA and functional activity. CONCLUSION: In RASMC, NO exposure results in upregulation of TF functional activity, antigen, and mRNA. This effect appears to be mediated by an @entity2451 -dependent pathway.

Regulation of tissue factor expression in smooth muscle cells with XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity2451 :: ('4790', 'Gene') :: ['NFkappaB', 'nuclear factor kappa-B'] @entity16475 :: ('MESH:D026423', 'Chemical') :: ['S-nitroso-N-acetylpenicillamine'] @entity13373 :: ('MESH:C020972', 'Chemical') :: ['pyrrolidine dithiocarbamate'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity35 :: ('10116', 'Species') :: ['Rat'] @entity3623 :: ('4843', 'Gene') :: ['inducible NO synthase']
@entity842 :: (MESH:D009569,Chemical) :: ['nitric oxide']
