In the quantitative proteomic studies, numerous in vitro and in vivo peptide labeling strategies have been successfully applied to measure differentially regulated protein and peptide abundance. These approaches have been proven to be versatile and repeatable in biological discoveries. @entity13140 metabolic labeling is one of these widely adopted and economical methods. However, due to the differential incorporation rates of @entity13140 or @entity38947 , the labeling results produce imperfectly matched isotopic envelopes between the heavy and light @entity1568 -labeled peptides. In the present study, we have modified the solid @entity958 growth medium to standardize the @entity13140 supply, which led to a uniform incorporation of @entity13140 into the whole plant protein complement. The incorporation rate (97.43  0.11%) of @entity13140 into @entity13140 -coded peptides was determined by correlating the intensities of peptide ions with the labeling efficiencies according to Gaussian distribution. The resulting actual incorporation rate (97.44%) and natural abundance of @entity13140 / @entity38947 -coded peptides are used to re-calculate the intensities of isotopic envelopes of differentially labeled peptides, respectively. A modified @entity13140 / @entity38947 stable isotope labeling strategy, SILIA, is assessed and the results demonstrate that this approach is able to differentiate the fold change in protein abundance down to 10%. The machine dynamic range limitation and purification step will make the precursor ion ratio deriving from the actual ratio fold change. It is suggested that the differentially mixed @entity13140 -coded and @entity38947 -coded plant protein samples that are used to establish the protein abundance standard curve should be prepared following a similar protein isolation protocol used to isolate the proteins to be quantitated.

Relative and accurate measurement of protein abundance using 15N stable isotope labeling in XXXX (SILIA).

@entity958 :: ('3702', 'Species') :: ['Arabidopsis'] @entity38947 :: ('CHEBI:36938', 'Chemical') :: ['(14)N'] @entity1568 :: ('MESH:D009584', 'Chemical') :: ['nitrogen'] @entity13140 :: ('CHEBI:36934', 'Chemical') :: ['(15)N']
@entity958 :: (3702,Species) :: ['Arabidopsis']
