OBJECTIVE: To observe the growth characteristics of @entity1 umbilical cord mesenchymal stem cells (hUCMSCs) cultured on the alginate gel scaffolds and to explore the feasibility of hUCMSCs-alginate dressing for wound healing. METHODS: hUCMSCs were separated from @entity1 umbilical cords and cultured in vitro. After the 4th passage cells were co-cultured with alginate gel (experimental group), the cell growth characteristics were observed under the inverted phase contrast microscope. @entity7 ( @entity7 ) content was measured and the number of cells was counted at 0, 3, 6, and 9 days after culture; and the cell migration capacity was observed. The hUCMSCs were cultured without alginated gel as control. The model of full-thickness skin defects was established in 32 8-week-old Balb/c male @entity19 and they were randomly divided into 4 groups (n=8): wounds were covered with hUCMSCs-alginate gel compound (MSC-gel group), cell supernatants-alginate gel compound (CS-gel group), 10% @entity4434 (FBS-gel group), and 0.01 mol/L PBS-alginate compound (PBS-gel group), respectively. Wound healing rates at 5, 10, and 15 days were observed and calculated; and the wound tissues were harvested for histological and immunohistochemical staining to assess new @entity272 at 15 days after operation. RESULTS: hUCMSCs grew well with grape-like proliferation on the alginate gel, but no cell migration was observed at 7 days after cultivation. @entity7 expression and cell number in experimental group were significantly less than those in control group at 3 days (P<0.05); then they gradually increased, and @entity7 expression and cell number were significantly more than those in control group at 9 days (P<0.05). The wound healing rates of MSC-gel and CS-gel groups were significantly higher than those of FBS-gel and PBS-gel groups at 5, 10, and 15 days (P<0.05). The squamous epithelium, fibroblasts, sebaceous glands, capillaries and @entity7 expression of the new skin in MSC-gel and CS-gel groups were significantly more than FBS-gel and PBS-gel groups (P<0.05). But there was no significance between MSC-gel and CS-gel groups (P>0.05). CONCLUSION: hUCMSCs can continuously express @entity7 in alginate gel, which is necessary for wound healing. The hUCMSCs-alginate compound is probably a good wound dressing.

[EXPERIMENTAL STUDY ON XXXX UMBILICAL CORD MESENCHYMAL STEM CELLS-ALGINATE WOUND DRESSING].

@entity1 :: ('9606', 'Species') :: ['human'] @entity7 :: ('7422', 'Gene') :: ['VEGF', 'Vascular endothelial growth factor'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity272 :: ('MESH:D012871', 'Disease') :: ['skin conditions'] @entity4434 :: ('MESH:D005198', 'Disease') :: ['FBS-alginate gel compound']
@entity1 :: (9606,Species) :: ['HUMAN']
