The role of matrix metalloproteases and their regulation in the pathology of @entity5479 is still unclear. Recently we have demonstrated that incubation of keratinocytes with @entity3540 debris and granulation tissue extracts causes induction of gelatinase B ( @entity1495 , @entity1495 ) secretion in vitro. Antibodies against a variety of growth factors revealed some inhibitory effect on @entity1495 induction, caused by debris or granulation tissue extracts. In order to investigate the coherence of growth factor expression and matrix metalloproteinase activity in vivo in @entity5479 , we performed quantitative gelatin zymographic analysis with tissue homogenates of 37 @entity3540 and nine external ear canal skin (EACS) samples. Furthermore we quantified levels of the cytokines @entity1192 , @entity1356 , @entity310 , @entity2533 and EGF present in tissue extracts, using enzyme-linked immunosorbent assays (ELISA), and correlated cytokine concentrations with gelatinolytic activities. Zymographic analysis revealed a highly heterogeneous expression of gelatinase A and B in @entity3540 specimens. As shown previously, @entity1495 , but not @entity1476 , was increased in @entity3540 when compared to EACS samples. ELISA studies revealed a significantly elevated @entity1192 level in @entity3540 . Regression analysis involving gelatinolytic activity and cytokine concentrations in tissue homogenates showed no statistically significant correlation between expression of gelatinases and the cytokines @entity1192 , @entity1356 , @entity310 , @entity2533 or EGF. The discrepancy between in vitro observations and the situation in vivo is discussed critically.

Up-regulation of matrix metalloprotease-9 in XXXX --correlations with growth factor expression in vivo?

@entity1356 :: ('3553', 'Gene') :: ['IL-1beta', 'IL1-beta'] @entity310 :: ('7124', 'Gene') :: ['TNF-alpha'] @entity1476 :: ('4313', 'Gene') :: ['MMP-2'] @entity5479 :: ('MESH:D018424', 'Disease') :: ['middle ear cholesteatoma'] @entity3540 :: ('MESH:D002781', 'Disease') :: ['cholesteatoma'] @entity2533 :: ('7040', 'Gene') :: ['TGF-beta'] @entity1192 :: ('3552', 'Gene') :: ['IL1-alpha', 'IL-1alpha'] @entity1495 :: ('4318', 'Gene') :: ['MMP-9', 'matrix metalloproteinase-9']
@entity5479 :: (MESH:D018424,Disease) :: ['middle ear cholesteatoma']
