BACKGROUND: various @entity98 assay methods are proposed to obtain the cell survival parameters. OBJECTIVE: determining the survival curve characteristics of two cancerous cells of interest based on a common and a novel @entity98 assay method after exposing them to ionizing radiation. METHODS: a common and a novel @entity98 assay method were used and compared for obtaining the F10B16 @entity320 and 4T1 @entity0 survivals after exposing them to ionizing radiation from a Co-60 machine. To obtain the survival parameters of the cells based on the common method, the cells were inoculated in 96-well plates. After irradiating the plates, the @entity98 assay was performed over the following days for a period of 8 days. Thereafter, the survival fraction was calculated from a simple equation for every day from which the best day was selected. To acquire the cells' survival parameters based on the novel method, extensive experiments were performed on a large number of samples. Then, the @entity98 assay was done in every day following various experimental treatments to acquire the exponential growth. Finally, the cells' survivals were determined by measuring the space between relevant growing curves. RESULTS: at low doses (<4Gy) the two @entity98 assay methods indicated the same results. However, at higher doses there were significant differences among the findings. CONCLUSION: both of the @entity98 methods indicated that the cells' responses are dependent on the dose levels used. Although the implementation of the common @entity98 assay method is simpler, the novel method seems to show more precise and reliable results at all levels of radiation doses.

The Cell Survival of F10B16 @entity320 and 4T1 XXXX Irradiated to Gamma Radiation Using the MTT Assay Based on Two Different Calculation Methods.

@entity0 :: ('MESH:D001943', 'Disease') :: ['breast adenocarcinoma'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma']
@entity0 :: (MESH:D001943,Disease) :: ['Breast Adenocarcinoma']
