BACKGROUND: Semaphorins (SEMAs) consist of a large family of secreted and membrane-anchored proteins that are important in neuronal pathfinding and axon guidance in selected areas of the developing nervous system. Of them, @entity68347 has been reported to have a chemotactic activity in neurogenesis and to be an immunomodulator; however, little is known about the relevance of @entity68347 in the behaviors of @entity957 ( @entity957 ). METHODS: We evaluated @entity68347 expression in @entity957 -derived cell lines and primary @entity957 samples using quantitative reverse transcriptase-polymerase chain reaction, immunoblotting, and semiquantitative immunohistochemistry (sq-IHC). In addition, @entity68347 knockdown cells (shSEMA7A cells) were used for functional experiments, including cellular proliferation, invasiveness, and migration assays. We also analyzed the clinical correlation between @entity68347 status and clinical behaviors in @entity1 with @entity957 . RESULTS: @entity68347 mRNA and protein were up-regulated significantly (P<0.05) in @entity957 -derived cell lines compared with @entity1 normal oral keratinocytes. The shSEMA7A cells showed decreased cellular growth by cell-cycle arrest at the G1 phase, resulting from up-regulation of cyclin-dependent kinase inhibitors ( @entity994 and @entity13525 ) and down-regulation of cyclins ( @entity4910 , cyclin E) and cyclin-dependent kinases ( @entity15308 , @entity8985 , and @entity14107 ); and decreased invasiveness and migration activities by reduced secretion of matrix metalloproteases (MMPs) ( @entity1476 , proMMP-2, pro-MMP-9), and expression of @entity1551 ( @entity1551 ). We also found inactivation of the @entity584 pathways, an upstream molecule of cell-cycle arrest at the G1 phase, and reduced secretion of MMPs in shSEMA7A cells. sq-IHC showed that @entity68347 expression in the primary OSCCs was significantly (P = 0.001) greater than that in normal counterparts and was correlated with primary tumoral size (P = 0.0254) and regional lymph node @entity3 (P = 0.0002). CONCLUSION: Our data provide evidence for an essential role of @entity68347 in tumoral growth and @entity3 in @entity957 and indicated that @entity68347 may play a potential diagnostic/therapeutic target for use in @entity1 with @entity957 .

Semaphorin7A Promotion of Tumoral Growth and XXXX in @entity1 Oral Cancer by Regulation of G1 Cell Cycle and Matrix Metalloproteases: Possible Contribution to Tumoral Angiogenesis.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity14107 :: ('1021', 'Gene') :: ['CDK6'] @entity1476 :: ('4313', 'Gene') :: ['MMP-2'] @entity957 :: ('MESH:D002294', 'Disease') :: ['OSCC', 'oral squamous cell carcinoma'] @entity4910 :: ('595', 'Gene') :: ['cyclin D1'] @entity8985 :: ('1019', 'Gene') :: ['CDK4'] @entity584 :: ('207', 'Gene') :: ['extracellular regulated kinase 1/2 and AKT'] @entity1551 :: ('4323', 'Gene') :: ['membrane type 1- MMP', 'MT1-MMP'] @entity15308 :: ('1017', 'Gene') :: ['CDK2'] @entity13525 :: ('1027', 'Gene') :: ['p27Kip1'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity68347 :: ('8482', 'Gene') :: ['SEMA7A'] @entity994 :: ('1026', 'Gene') :: ['p21Cip1']
@entity3 :: (MESH:D009362,Disease) :: ['Metastasis']
