BACKGROUND: Emergence of multidrug-resistant @entity3064 has resulted in the treatment failure of related @entity281 and an increase in @entity1 mortality. The presence of class D b-lactamases (oxacillinases) in this organism is an important mechanism underlying resistance to @entity8545 antibiotics. OBJECTIVES: The aim of this work was to investigate the correlation between oxacillinase gene carriage and genetic fingerprints in @entity2892 -resistant burn and non-burn isolates of A. baumannii. MATERIALS AND METHODS: Fifty-eight A. baumannii isolates were collected from October 2011 to April 2012, which included 28 burn isolates from Shahid Motahari Hospital and 30 non-burn isolates from Imam Hossein Hospital. The minimum inhibitory and minimum bactericidal concentrations (MIC and MBC) of @entity2892 were measured by the broth microdilution method. The presence of oxacillinase genes ( @entity15799 -, OXA-24-, OXA-51-, and OXA-58-like genes) was shown using type-specific primers and PCR. Genetic profiles were generated by RAPD-PCR fingerprinting. RESULTS: @entity15799 was observed in 81% of the isolates and its distribution was similar within the two groups. The presence of OXA-51 was shown in 58.6% of the isolates, of which most were burn isolates (67.6%). OXA-24 was present in 20.7% of the isolates, all belonging to the burn group; OXA-58 was not observed in any of the isolates. RAPD-PCR fingerprints revealed two clusters at a similarity level of 70% (A, B). At a similarity level of 85%, nine different groups were observed for burn and non-bun isolates. CONCLUSIONS: Our results showed that bla @entity15799 was the most prevalent gene, followed by bla OXA-51 , among the burn and non-burn clinical isolates of A. baumannii. Bla OXA-24 -like genes were detected at a lower level and were only found among the burn isolates, which also showed higher heterogeneity compared to the non-burn group.

Correlation of Oxacillinase Gene Carriage With the Genetic Fingerprints of XXXX -Resistant Clinical Isolates of @entity3064 .

@entity1 :: ('9606', 'Species') :: ['patient'] @entity2892 :: ('MESH:D015378', 'Chemical') :: ['imipenem'] @entity8545 :: ('D047090', 'Chemical') :: ['beta-lactam'] @entity15799 :: ('20472025', 'Gene') :: ['OXA-23'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infections'] @entity3064 :: ('470', 'Species') :: ['Acinetobacter baumannii']
@entity2892 :: (MESH:D015378,Chemical) :: ['Imipenem']
