Several important Pistacia species such as P. vera have been traditionally used for treating a wide range of diseases (for instance, @entity409 ). There is a relative lack of research into pharmacological aspects of pistachio hull. Hence, this study was aimed at investigating whether pistachio rosy hull (PRH) extract exerts apoptotic impacts on HepG2 @entity96 cell line. In order to evaluate cell viability and apoptosis in response to treatment with the extract, @entity98 assay and @entity4404 -fluorescein/ @entity2667 (PI) double staining were performed, respectively. Moreover, molecular mechanism of apoptosis induced by the extract was determined using @entity1 apoptosis PCR array. Our findings showed that PRH extract treatment reduced cell viability (IC50 ~ 0.3 mg/ml) in a dose-dependent manner. Flow cytometric analysis revealed that the extract significantly induced apoptosis in HepG2 cells. In addition, quantitative PCR array results demonstrated the regulation of a considerable number of apoptosis-related genes belonging to the TNF, @entity588 , @entity6307 , TRAF, and caspase families. We observed altered expression of both pro-apoptotic and anti-apoptotic genes associated with the extrinsic and intrinsic apoptosis signaling pathways. These results suggest that the aqueous extract of PRH possesses apoptotic activity through cytotoxic and apoptosis-inducing effects on HepG2 cells.

Induction of apoptosis by pistachio (Pistacia vera L.) hull extract and its molecular mechanisms of action in XXXX hepatoma cell line HepG2.

@entity1 :: ('9606', 'Species') :: ['human'] @entity6307 :: ('248', 'Gene') :: ['IAP'] @entity588 :: ('596', 'Gene') :: ['BCL2'] @entity2667 :: ('MESH:D011419', 'Chemical') :: ['propidium iodide'] @entity4404 :: ('308', 'Gene') :: ['Annexin-V'] @entity96 :: ('MESH:D008113', 'Disease') :: ['liver cancer'] @entity409 :: ('MESH:D008107', 'Disease') :: ['liver-related disorders'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT']
@entity1 :: (9606,Species) :: ['human']
