@entity43 is the most lethal gynecologic malignancy, however underlying molecular events remain elusive. Expression of @entity1 chorionic gonadotropin b subunit (b-hCG) is clinically significant for both trophoblastic and nontrophoblastic @entity5 ; however, whether b-hCG facilitates ovarian epithelial cell tumorigenic potential remains uncharacterized. Immortalized nontumorigenic ovarian epithelial T29 and T80 cells stably overexpressing b-hCG were examined for alterations in cell cycle and apoptotic status by flow cytometry, expression of proteins regulating cell cycle and apoptosis by Western blot, proliferation status by @entity98 assay, anchorage-independent colony formation, and @entity19 @entity5 formation. Immunoreactivity for b-hCG was evaluated using @entity19 xenografts and on @entity1 normal ovarian, fallopian tube, endometrium, and @entity43 tissues. T29 and T80 cells overexpressing b-hCG demonstrated significantly increased proliferation, anchorage-independent colony formation, prosurvival @entity1836 protein expression, G2-checkpoint progression, elevated cyclins E/D1 and @entity37020 , and decreased apoptosis. Collectively, these transformational alterations in phenotype facilitated increased xenograft tumorigenesis (P < 0.05). Furthermore, b-hCG immunoreactivity was elevated in @entity43 , compared with normal epithelial expression in ovaries, fallopian tube, and endometrium (P < 0.001). Our data indicate that elevated b-hCG transforms ovarian surface epithelial cells, facilitating proliferation, cell cycle progression, and attenuated apoptosis to promote tumorigenesis. Our results further decipher the functional role and molecular mechanism of b-hCG in @entity43 . b-hCG may contribute to @entity43 etiology, which introduces a new therapeutic intervention target for @entity43 .

Overexpression of the b subunit of XXXX chorionic gonadotropin promotes the transformation of @entity1 ovarian epithelial cells and ovarian tumorigenesis.

@entity43 :: ('MESH:D010051', 'Disease') :: ['ovarian carcinoma', 'Ovarian carcinoma', 'malignant ovarian tumors', 'ovarian cancer'] @entity1 :: ('9606', 'Species') :: ['human'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancers'] @entity1836 :: ('598', 'Gene') :: ['Bcl-X(L)'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity37020 :: ('1017;1019;1021', 'Gene') :: ['Cdk 2/4/6']
@entity1 :: (9606,Species) :: ['human']
