OBJECTIVE: To assess the viability and function of @entity1 sperm in electrolyte-free cold preservation. DESIGN: Prospective comparative study. SETTING: Andrology laboratory of our hospital. @entity1 (S): Ten semen samples obtained from @entity1 attending our @entity249 clinic. INTERVENTION(S): Ejaculated sperm were washed using the electrolyte-free Percoll gradient and were then preserved in 0.33 M @entity413 solution, 0.16 M @entity1219 solution, 0.16 M @entity4338 solution at 4 degrees C for 4 weeks. As a control, TEST (TES and Tris) yolk buffer (TYB) was added to the ejaculated semen and preserved at 4 degrees C. MAIN OUTCOME MEASURE(S): Sperm tail morphology, motility, viability (eosin-Y stain), and the concentration of @entity855 ( @entity855 ) were analyzed. RESULT(S): The number of sperm with normal tail form and the motility of sperm preserved in @entity413 solution (electrolyte-free cold preservation) were significantly (P < 0.01) higher for 4 weeks than those of sperm preserved in the other three media. The sperm viability in @entity413 solution was 75.5%, 65.4%, and 51.3%, after 1, 2, and 4 weeks, respectively. The @entity855 concentration after 1, 2, and 4 weeks remained 64.2%, 53.0%, and 4.3% of the prestorage value, respectively, in the sperm stored in @entity413 solution. CONCLUSION(S): The morphology, motility, viability, and @entity855 concentration of sperm in electrolyte-free cold preservation were substantially better than those in @entity1219 solution, @entity4338 solution, or TYB for 2 weeks.

Viability and function of XXXX sperm in electrolyte-free cold preservation.

@entity1 :: ('9606', 'Species') :: ['human', 'patients', 'PATIENT'] @entity249 :: ('MESH:D007246', 'Disease') :: ['infertility'] @entity1219 :: ('CHEBI:26710', 'Chemical') :: ['NaCl'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity4338 :: ('CHEBI:32588', 'Chemical') :: ['KCl'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['adenosine triphosphate', 'ATP']
@entity1 :: (9606,Species) :: ['human']
