@entity54 is the most common aetiological fungus responsible for @entity1 @entity57 . This study investigated the primary contact between Langerhans cells (LC), corresponding to dendritic cells present in pulmonary mucosa and live conidia of A. fumigatus. LC play a key role in antigen presentation for initiation of the primary T cell response. In vitro-generated LC (iLC) were differentiated from cultured @entity1 cord blood @entity2280 + cells and incubated at 4 degrees C or 37 degrees C with @entity2822 ( @entity2823 )-stained conidia or control latex beads. In vitro, conidia were shown by microscopy and cytometry to adhere to iLC in a dose- and time-dependent manner. This adhesion was not limited to iLC because interstitial dendritic and other cells also fluoresced in the presence of conidia- @entity2823 . A lectin other than @entity3913 receptor-type lectin was demonstrated to be responsible of conidial binding. Inhibition of binding was observed with heterologous galactomannan and @entity1098 , indicating a C-lectin-like receptor with galactomannan structure specificity. After binding only a few conidia were internalized in acidic vesicles, as indicated by the cessation of conidial fluorescence. Conidial binding was followed by activation and maturation of iLC, suggesting that LC present in the lung may play a role in cellular host defence against @entity2381 .

Binding of live conidia of @entity54 activates in vitro-generated XXXX Langerhans cells via a lectin of galactomannan specificity.

@entity1 :: ('9606', 'Species') :: ['human'] @entity57 :: ('MESH:D055732', 'Disease') :: ['pulmonary aspergilloses'] @entity2822 :: ('CHEBI:37926', 'Chemical') :: ['fluorescein-isothiocyanate'] @entity2381 :: ('MESH:D001228', 'Disease') :: ['aspergilloses'] @entity2823 :: ('MESH:D016650', 'Chemical') :: ['FITC'] @entity54 :: ('746128', 'Species') :: ['Aspergillus fumigatus'] @entity3913 :: ('MESH:D008358', 'Chemical') :: ['mannose'] @entity2280 :: ('947', 'Gene') :: ['CD34'] @entity1098 :: ('MESH:D004492', 'Chemical') :: ['EDTA']
@entity1 :: (9606,Species) :: ['human']
