The objectives of this study were to evaluate the morphology and biomechanical function of Achilles tendons regenerated using knitted @entity10124 ( @entity7050 ) loaded with bone marrow stromal cells (bMSCs). The animal model used was that of an adult female New Zealand White @entity95 with a 10-mm gap defect of the Achilles tendon. In group I, 19 hind legs with the created defects were treated with allogeneic bMSCs seeded on knitted @entity7050 scaffold. In group II, the @entity924 in 19 hind legs were repaired using the knitted @entity7050 scaffold alone, and in group III, 6 hind legs were used as normal control. The tendon-implant constructs of groups I and II were evaluated postoperatively at 2, 4, 8, and 12 weeks using macroscopic, histological, and immunohistochemical techniques. In addition, specimens from group I (n = 7), group II (n = 7), and group III (n = 6) were harvested for biomechanical test 12 weeks after surgery. Postoperatively, at 2 and 4 weeks, the histology of group I specimens exhibited a higher rate of tissue formation and remodeling as compared with group II, whereas at 8 and 12 weeks postoperation, the histology of both group I and group II was similar to that of native tendon tissue. The wound sites of group I healed well and there was no apparent lymphocyte infiltration. Immunohistochemical analysis showed that the regenerated tendons were composed of collagen types I and type III fibers. The tensile stiffness and modulus of group I were 87 and 62.6% of normal tendon, respectively, whereas those of group II were about 56.4 and 52.9% of normal tendon, respectively. These results suggest that the knitted @entity7050 biodegradable scaffold loaded with allogeneic bone marrow stromal cells has the potential to regenerate and repair gap defect of Achilles tendon and to effectively restore structure and function.

Knitted @entity10124 scaffold loaded with bone marrow stromal cells in repair and regeneration of XXXX Achilles tendon.

@entity7050 :: ('MESH:C043435', 'Chemical') :: ['PLGA'] @entity924 :: ('MESH:D052256', 'Disease') :: ['Achilles tendon defects'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity10124 :: ('CHEBI:53493', 'Chemical') :: ['poly-lactide-co-glycolide']
@entity95 :: (9986,Species) :: ['rabbit']
