To examine DNA abnormalities in @entity150 induced by @entity17077 (BBN) in female @entity35 , using image cytometric DNA analysis and cytogenetics. Thirty female @entity35 were exposed to BBN in their drinking water for 20 weeks. One group of 10 animals served as controls. The animals exposed to BBN were killed at a rate of two per week, with the bladder being collected under aseptic conditions and those @entity5 with exophytic growth removed. The nuclear DNA content of the @entity5 was evaluated using image cytometric analysis. In two @entity35 part of the @entity5 pieces was stipulated for culturing. Cytogenetic analysis was performed on at least 30 cells from each cell population and on both @entity5 . @entity1377 were classified as low grade and high grade. DNA ploidy studies were carried out on 28 low-grade and 21 high-grade @entity1377 . Histograms obtained by image analysis showed that a normal urothelium was diploid; 28.6% and 100% of low-and high-grade @entity1377 were @entity2007 respectively. Both @entity5 used for cell culture showed multiple numerical and structural chromosome alterations and several marker chromosomes. Image cytometric DNA analysis proved to be a good and reliable method for examining DNA alterations in @entity150 . The present findings establish that the DNA content is statistically different between low-grade and high-grade @entity1377 and that deviation from the diploid number is markedly higher in the high-grade ones. In addition, the occurrence of marker chromosomes seems to be related to the @entity5 .

DNA study of @entity150 chemically induced by @entity17077 in Fisher XXXX .

@entity1377 :: ('MESH:D002291', 'Disease') :: ['papillary carcinomas', 'Papillary carcinomas'] @entity2007 :: ('MESH:D000782', 'Disease') :: ['aneuploid'] @entity5 :: ('MESH:D009369', 'Disease') :: ['aggressiveness of the tumour', 'tumours', 'tumour'] @entity17077 :: ('MESH:D002085', 'Chemical') :: ['N-butyl-N-(4-hydroxybutyl) nitrosamine'] @entity150 :: ('MESH:D001749', 'Disease') :: ['bladder papillary tumours', 'papillary bladder carcinomas'] @entity35 :: ('10116', 'Species') :: ['rats']
@entity35 :: (10116,Species) :: ['rats']
