BACKGROUND: T helper (Th) cells immune regulation is important for the pathogenesis of @entity3052 ( @entity3052 ). Recurrent @entity854 in @entity3052 peripheral blood were reported, while the comprehensive status of various Th subsets is rarely investigated in bone marrow (BM) microenvironment which is the origin of @entity3052 leukemic blast cells. METHODS: BM was extracted from 48 @entity18119 ( @entity18119 ), 34 complete-remission (CR), 19 relapsed-refractory @entity3052 @entity1 and 15 controls. Slight @entity68 deficiency @entity417 @entity1 were used as controls. Th subsets frequencies were examined by flow cytometry. BM plasma Th-associated cytokines levels were determined by ELISA. The expression of key transcription factor was examined by RT-PCR. RESULTS: Th22, Th17, @entity1198 , Th2 cells, @entity13236 and RORC expression were significantly decreased, while Treg cells, related cytokine @entity688 and transcription factor @entity403 were markedly elevated in @entity18119 compared to CR @entity1 or controls. Meanwhile, the imbalanced @entity1198 /Th2 and Th17/Treg ratio were observed in @entity18119 and relapsed-refractory @entity1 . Negative correlation between @entity1198 or Th2 and peripheral WBC, between Th17/Treg or @entity1198 /Th2 and leukemic blast existed in @entity18119 @entity1 . Moreover, chemotherapy ameliorated these variations. CONCLUSION: Th subsets in BM are distinct for different stages of @entity3052 and chemotherapy partly ameliorates the abnormality. Our findings suggest that these cells and cytokines may be implicated in @entity3052 pathogenesis and provided therapeutic insights.

The Profile of T Helper Subsets in Bone Marrow Microenvironment Is Distinct for Different Stages of Acute Myeloid Leukemia XXXX and Chemotherapy Partly Ameliorates These Variations.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity18119 :: ('MESH:D019952', 'Disease') :: ['ND', 'newly-diagnosed'] @entity417 :: ('MESH:D000740', 'Disease') :: ['anemia'] @entity13236 :: ('50616', 'Gene') :: ['IL-22'] @entity1198 :: ('51497', 'Gene') :: ['Th1'] @entity3052 :: ('MESH:D015470', 'Disease') :: ['acute myeloid leukemia', 'AML'] @entity854 :: ('MESH:D018376', 'Disease') :: ['Th abnormalities'] @entity403 :: ('50943', 'Gene') :: ['Foxp3'] @entity688 :: ('3586', 'Gene') :: ['IL-10'] @entity68 :: ('MESH:D007501', 'Chemical') :: ['iron']
@entity1 :: (9606,Species) :: ['Patients']
