Among all fruits tested in vitro for their anti-platelet property, @entity523 had the highest activity followed by grapefruit, @entity2487 , and strawberry, whereas pear and apple had little or no activity. @entity523 extract (20-50 microl of 100% juice) inhibited both @entity857 - and collagen-induced aggregation by up to 70% but could not inhibit @entity1426 -induced @entity1423 and concomitant @entity2871 synthesis under similar experimental conditions. The anti-platelet components (MW <1000 Da) in tomatoes are water soluble, heat stable and are concentrated in the yellow fluid around the seeds. The active fractions were separated using gel filtration and HPLC. The aqueous fraction (110 000 xg supernatant) of tomatoes containing anti-platelet activity was subjected to gel filtration column chromatography (Biogel P2 column). The activity was fractionated into two peaks, peak-3 and peak-4 (major peak). Subsequently, peak-4 was further purified by HPLC using a reversed-phase column. NMR and mass spectroscopy studies indicated that peak F2 (obtained from peak 4) contained @entity859 and @entity3183 . Deamination of peak F2 with @entity859 deaminase almost completely abolished its anti-platelet activity, confirming the presence of @entity859 in this fraction. In comparison, deamination of peak-4 resulted in only partial loss of inhibitory activity while the activity of peak-3 remained unaffected. These results indicate that tomatoes contain anti-platelet compounds in addition to @entity859 . Unlike @entity117 , the @entity523 -derived compounds inhibit thrombin-induced @entity1423 . All these data indicate that @entity523 contains very potent anti-platelet components, and consuming tomatoes might be beneficial both as a preventive and therapeutic regime for @entity65 .

Effects of XXXX extract on human @entity1423 in vitro.

@entity2871 :: ('CHEBI:26995', 'Chemical') :: ['thromboxane'] @entity859 :: ('MESH:D000241', 'Chemical') :: ['adenosine'] @entity1423 :: ('MESH:D001791', 'Disease') :: ['platelet aggregation'] @entity2487 :: ('3656', 'Species') :: ['melon'] @entity117 :: ('MESH:D001241', 'Chemical') :: ['aspirin'] @entity1426 :: ('MESH:D016718', 'Chemical') :: ['arachidonic acid'] @entity523 :: ('4081', 'Species') :: ['tomato', 'Tomato'] @entity3183 :: ('MESH:D003562', 'Chemical') :: ['cytidine'] @entity857 :: ('MESH:D000244', 'Chemical') :: ['ADP'] @entity65 :: ('MESH:D002318', 'Disease') :: ['cardiovascular disease']
@entity523 :: (4081,Species) :: ['tomato']
