BACKGROUND: With expansion of @entity2 screening by computed tomography (CT) and increased incidence of @entity420 , we encountered more peripheral nodules to be identified. We evaluated detection rates of the melanoma antigen genes (MAGE) RT-nested PCR using a common MAGE A1-6 primer in bronchoscopic washing samples from @entity1 with bronchoscopically invisible peripheral nodules, and compared with those of conventional cytology and percutaneous needle aspiration biopsy ( @entity45808 ). MATERIALS AND METHODS: Forty-two @entity1 with bronchoscopically invisible peripheral lung nodules were studied. Bronchoscopic washing specimens were collected by instilling 20ml of normal saline into a @entity5 bearing segment and retrieving samples. Samples were split and analyzed by MAGE RT-PCR and conventional cytology. A @entity45808 was performed in all but two @entity1 . Twenty-eight lesions were diagnosed as @entity2 and 14 as @entity358 . We evaluated MAGE A1-6 RT-nested PCR, cytology, and @entity45808 results and analyzed them according to histologic cell types and @entity5 sizes. RESULTS: In bronchial washing samples, the detection rates of MAGE A1-6 RT-nested PCR (67.9%) were higher than that of conventional cytology (21.4%, p=.04) and similar to that of @entity45808 (73.1%, p=.45) in 28 @entity5 @entity1 . In terms of histologic types, the detection rates of MAGE A1-6 RT-nested PCR, conventional cytology, and @entity45808 were 78.6%, 21.4% and 64.3% in @entity420 , 70%, 20% and 77.8% in @entity957 , respectively. The sensitivity of conventional cytology was significantly lower than that of MAGE RT-nested PCR or @entity45808 regardless of @entity5 histology p=.04, p=.025, respectively, in @entity420 ; p=.035, p=.04, respectively, in @entity957 ). In terms of @entity5 size, the corresponding detection rates were 73.3%, 6.7% and 73.3% for @entity5 smaller than 3cm (N=15), and 61.5%, 38.5% and 72.7% for @entity5 larger than 3cm (N=13). CONCLUSIONS: MAGE A1-6 RT-nested PCR showed higher detection rates in the bronchial washes of peripheral @entity2 @entity1 than conventional cytology testing. This method is simple and robust, and it could be effectively utilized as a peripheral @entity2 detection tool in clinical laboratories.

The detection of peripheral XXXX by MAGE A1-6 RT-nested PCR in bronchial washing specimens.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'tumors'] @entity45808 :: ('MESH:C005805', 'Chemical') :: ['PCNB'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung cancers', 'lung cancer'] @entity957 :: ('MESH:D002294', 'Disease') :: ['squamous cell carcinoma'] @entity358 :: ('MESH:D008171', 'Disease') :: ['benign lung diseases'] @entity420 :: ('MESH:D000230', 'Disease') :: ['adenocarcinoma']
@entity2 :: (MESH:D008175,Disease) :: ['lung cancer']
