Elevated telomerase activity is an important molecular signature of @entity5 cells and primitive cells in regenerative tissues. However, isolation of single living cells with endogenous telomerase activity has not yet been possible. Here, we developed @entity9504 serotype 35 tropism-based vectors encoding destabilized enhanced green fluorescence protein with a half-life of 2 h (d2EGFP) driven by the @entity1 telomerase reverse transcriptase ( @entity2215 ) promoter. As assessed in telomerase-positive or -negative cell lines, the d2EGFP expression positively correlated with @entity2215 transcript content and telomerase activity. In @entity1968 -induced differentiating HL-60 cells, the d2EGFP expression is diminished in the same manner as the @entity2215 expression. Individual cells from HeLa and HL-60 cell lines exhibited heterogeneous d2EGFP expression, which was cell cycle dependent, as the sorted d2EGFP+ HL-60 cells contained twice as many cells in S/G2/M phase of the cell cycle compared with the d2EGFP- HL-60 cells. However, both cell populations exhibited the same proliferation and regeneration capacities. Heterogeneous d2EGFP expression was also detected in xenograft @entity463 multiforme cells with @entity5 formation capacity. Thus, d2EGFP expression reported cell cycle- and differentiation stage-dependent @entity2215 expression. Our study facilitates isolation and characterization of single living cells with telomerase activity.

Detection of cell cycle- and differentiation stage-dependent @entity1 telomerase reverse transcriptase expression in single living XXXX cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity9504 :: ('MESH:D000257', 'Disease') :: ['adenovirus'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity2215 :: ('7015', 'Gene') :: ['hTERT'] @entity463 :: ('MESH:D005909', 'Disease') :: ['glioblastoma'] @entity1968 :: ('MESH:D014212', 'Chemical') :: ['retinoic acid']
@entity5 :: (MESH:D009369,Disease) :: ['cancer']
