Mesenchymal stem cells (MSCs) are multipotent progenitor cells exerting immunomodulatory effects on cells of the innate and adaptive immune system. It has been shown that an inflammatory milieu is required for the activation of MSC-mediated immunomodulation, and @entity379 ( @entity379 ) plays an important role in this process. We determined the influence of @entity379 on @entity1 adipose-derived stem cells (ASCs) and @entity1 amniotic mesenchymal stromal cells (hAMSCs). We further evaluated the effect of MSCs on stimulated T-cells and peripheral blood mononuclear cells (PBMCs) in a cell-contact independent setting. On @entity379 treatment, ASCs and hAMSCs possessed significantly higher antiproliferative properties and showed surface characteristics of nonprofessional antigen presenting cells (HLA-DR(+) @entity2936 (med+) @entity2071 (high)) with a possible regulatory phenotype ( @entity4634 (+) @entity14797 (+)). The effect of ASCs and hAMSCs on cytokine secretion and T-cell activation was dependent on stimulation method and cellular context. Although ASCs and hAMSCs highly inhibited cytokine secretion of stimulated PBMCs, this was not observed in the case of purified T-cells. The presence of ASCs even favored the secretion of pro-inflammatory cytokines including @entity379 by T-cells, although T-cell proliferation was efficiently inhibited. Further, ASCs enhanced the number of @entity7986 (+) T-cells independent of the stimuli and cellular context. Interestingly, ASCs significantly suppressed @entity7211 expression on phytohemagglutinin stimulated PBMCs but had no effect on aCD3/aCD28 stimulated cells. Depending on the stimulation method and cellular context, immune cells create a specific cytokine milieu in vitro, thus differently influencing MSCs and, in turn, affecting their action on immune cells.

XXXX mesenchymal stem cells from adipose tissue and amnion influence T-cells depending on stimulation method and presence of other immune cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity4634 :: ('29126', 'Gene') :: ['PD-L1'] @entity2936 :: ('958', 'Gene') :: ['CD40'] @entity379 :: ('3458', 'Gene') :: ['IFN-y', 'interferon-y'] @entity7211 :: ('3669', 'Gene') :: ['CD25'] @entity2071 :: ('3383', 'Gene') :: ['CD54'] @entity14797 :: ('80380', 'Gene') :: ['PD-L2'] @entity7986 :: ('969', 'Gene') :: ['CD69']
@entity1 :: (9606,Species) :: ['Human']
