UNASSIGNED: @entity375 extract (YE) is known to greatly enhance @entity1 cell culture performances, but its undefined composition decreases process reliability. Accordingly, in the present study, the nature of YE compounds involved in the improvement of recombinant CHO cell growth and IgG production was investigated. First, the benefits of YE were verified, revealing that it increased maximal concentrations of viable cells and IgG up to 73 and 60%, respectively compared to a reference culture. Then, the analyses of YE composition highlighted the presence of molecules such as @entity1522 , vitamins, salts, @entity21466 , and @entity413 that were contained in reference medium, while others including peptides, @entity7970 , polysaccharides, and nucleic acids were not. Consequently, YE was fractionated by a nanofiltration process to deeper evaluate its effects on CHO cell cultures. The YE molecules already contained in reference medium were mainly isolated in the permeate fraction together with @entity7970 and short peptides, while other molecules were concentrated in the retentate. Permeate, which was free of macromolecules, exhibited a similar positive effect than raw YE on maximal concentrations. Additional studies on cell energetic metabolism underlined that @entity9881 and @entity12274 in permeate were used as an efficient source of nitrogenous substrates.    2015 American Institute of Chemical Engineers Biotechnol. Prog., 2015.

Fractionation of XXXX extract by nanofiltration process to assess key compounds involved in CHO cell culture improvement.

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity7970 :: ('MESH:D014199', 'Chemical') :: ['trehalose'] @entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity12274 :: ('CHEBI:47923', 'Chemical') :: ['tripeptides'] @entity375 :: ('4932', 'Species') :: ['Yeast'] @entity9881 :: ('MESH:D004151', 'Chemical') :: ['dipeptides'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity21466 :: ('CHEBI:18282', 'Chemical') :: ['nucleobase']
@entity375 :: (4932,Species) :: ['yeast']
