Even though tremendous advances have been made in the treatment of @entity5 during the past decades, success rate among @entity5 @entity1 is still dismal largely due to problems associated with chemo/radio-resistance and relapse. Emerging evidence has indicated that @entity5 stem cells (CSCs) are behind the resistance and recurrence problems, but our understanding of their regulation is limited. Rapid reversible changes of CSC-like cells within @entity5 may result from the effect of biological mediators found in @entity5 microenvironment. Here we show how @entity842 (NO), a key cellular modulator whose level is elevated in many @entity5 , affects CSC-like phenotypes of @entity1 @entity1149 ( @entity1149 ) H292 and @entity1 cells. Exposure of NO gradually altered the cell morphology towards mesenchymal stem-like shape. NO exposure promoted CSC-like phenotype indicated by increased expression of known CSC markers, @entity5439 and @entity14215 , in the exposed cells. This effect of NO on stemness was reversible after cessation of the NO treatment for 7 days. Furthermore, such effect was reproducible using another NO-donor, @entity16475 (SNAP). Importantly, inhibition of NO by known NO scavenger 2-(4-carboxy-phenyl)-4,4,5,5 tetramethylimidazoline-1-oxy-3-oxide ( @entity12739 ) strongly inhibited CSC-like aggressive cellular behavior and marker expression. Lastly, we unveiled the underlying mechanism of NO action through the activation of caveolin-1 ( @entity3895 -1) which is upregulated by NO and is responsible for the aggressive behavior of the cells, including anoikis resistance, anchorage-independent cell growth, and increased cell migration and invasion. These findings indicate a novel role of NO in CSC regulation and its importance in aggressive @entity5 behaviors through @entity3895 -1 upregulation.

@entity842 induces Cancer Stem Cell-like Phenotypes in XXXX Lung Cancer Cells.

@entity1 :: ('9606', 'Species') :: ['human', 'patients', 'H460'] @entity5439 :: ('8842', 'Gene') :: ['CD133'] @entity16475 :: ('MESH:D026423', 'Chemical') :: ['S-nitroso-N-acetylpenicillamine'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancers', 'cancer', 'tumors'] @entity12739 :: ('MESH:C079391', 'Chemical') :: ['PTIO'] @entity1149 :: ('MESH:D002289', 'Disease') :: ['NSCLC', 'non-small-cell lung carcinoma'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity14215 :: ('216', 'Gene') :: ['ALDH1A1'] @entity3895 :: ('10537', 'Species') :: ['Cav']
@entity1 :: (9606,Species) :: ['Human']
