UNASSIGNED: Antinuclear antibodies (ANAs) are important biomarkers in the diagnosis of @entity792 in @entity1 ; however, the diagnostic performance of ANA in nonclinical safety studies are not well understood. Here, we studied the use of ANAs as potential nonclinical biomarkers for @entity792 ( @entity792 ) using a Hep-2 based indirect immunofluorescence assay (IFA). Initially, MRL-fas(lpr)/J @entity19 and @entity3143 -treated @entity35 were used as SLE-positive models. Serum samples obtained from 94 normal @entity19 or 204 normal @entity35 aged one to four months served as the negative control. The IFA effectively distinguished ANAs-positive samples in both species with a cut-off titer of 1:100. Brown @entity35 were treated with 450mg/kg @entity2361 for 30 consecutive days. ANAs were generated and corresponded with @entity792 development. @entity1 Hep-2 cells, @entity19 Neuro 2A cells, and @entity3569 Lung cells served as antigen from different species, which were found cross-reactive with ANA-positive serum samples from @entity19 , @entity35 , and @entity1 without any differences in diagnosis. This methodology showed no species-specificity for ANA detection. Furthermore, we found approximately 20 percentage of the @entity19 aged seven to eight months demonstrated age-related ANAs, which was consistent with @entity1 . Overall, our findings demonstrated the use of ANA detection using IFA in the nonclinical diagnosis of @entity19 @entity792 , and age-related ANAs should be considered when aged animals are used.

Hep-2 cell based indirect immunofluorescence assay for antinuclear antibodies as a potential diagnosis of XXXX in nonclinical toxicity testing.

@entity1 :: ('9606', 'Species') :: ['humans', 'Human'] @entity3143 :: ('MESH:D008627', 'Chemical') :: ['HgCl2'] @entity19 :: ('10090', 'Species') :: ['mice', 'murine'] @entity2361 :: ('MESH:D010396', 'Chemical') :: ['d-penicillamine'] @entity35 :: ('10116', 'Species') :: ['Norway rats', 'rats'] @entity792 :: ('MESH:D001327', 'Disease') :: ['DIA', 'drug-induced autoimmunity', 'autoimmune diseases'] @entity3569 :: ('10029', 'Species') :: ['Chinese Hamster']
@entity792 :: (MESH:D001327,Disease) :: ['drug-induced autoimmunity']
