UNASSIGNED: Highly malignant @entity1313 harbor the aberrant propensity for aerobic glycolysis, the excessive conversion of @entity413 to @entity880 even in the presence of ample tissue @entity26 . @entity880 is rapidly effluxed to the @entity5 microenvironment via a group of plasma-membrane transporters denoted @entity3338 transporters (MCTs) to prevent "self-poisoning." One isoform, @entity3341 , has the highest affinity for @entity880 and thus should have the ability to respond to microenvironment conditions such as @entity519 , @entity880 , and @entity8487 to help maintain high glycolytic flux in the @entity5 . Yet, @entity3341 is considered to not respond to @entity519 , which is counterintuitive. Its response to @entity5 @entity880 has not been reported. In this report, we experimentally identify the transcription initiation site/s for @entity3341 in astrocytes (normal) and @entity11 ( @entity5 ). We then use a BACmid library to isolate a 4.2-kbp @entity3341 promoter-exon I region and examine promoter response to glycolysis-mediated stimuli in @entity11 cells. Reporter analysis of nested-promoter constructs indicated response of @entity3341 to @entity519 , @entity8487 , @entity880 , and @entity413 , the major physiological "players" that facilitate a @entity5 's growth and proliferation. Immunoblot analysis of native @entity3341 expression under altered @entity8487 and @entity519 reflected the reporter data. The @entity8487 -mediated gene-regulation studies we describe are the first to record @entity8108 -based reporter studies for any @entity1 system and demonstrate the exquisite response of the @entity3341 gene to minute changes in @entity5 @entity8487 . Identical promoter usage also provides the first evidence of astrocytes harnessing the same gene regulatory regions to facilitate astrocyte-neuron @entity880 shuttling, a metabolic feature of normal brain.

@entity8487 , @entity880 , and XXXX : Reciprocity in Regulating High-Affinity @entity3338 Transporter Expression in Glioblastoma.

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity8487 :: ('MESH:D010677', 'Disease') :: ['pH'] @entity1313 :: ('MESH:D001932', 'Disease') :: ['brain tumors'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity3341 :: ('9194', 'Gene') :: ['MCT2'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity11 :: ('MESH:D005910', 'Disease') :: ['glioma'] @entity519 :: ('MESH:D000860', 'Disease') :: ['hypoxia'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactic acid', 'lactate', 'Lactic acid'] @entity8108 :: ('D006859', 'Chemical') :: ['H(+)'] @entity3338 :: ('CHEBI:35757', 'Chemical') :: ['monocarboxylate'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity519 :: (MESH:D000860,Disease) :: ['Hypoxia']
