Expression of some members of the cadherin family is reduced in several @entity1 @entity5 , and @entity20197 (H-cadherin), located on chromosome 16q24.2-3, may function as a @entity5 suppressor gene. In @entity1 @entity5 , loss of expression of many @entity5 suppressor genes occurs by aberrant promoter region methylation. We examined the methylation status of the @entity20197 promoter in @entity0 and correlated it with mRNA expression using methylation-specific PCR and reverse transcription-PCR. Methylation was frequent in primary @entity0 (18 of 55, 33%) and cell lines (7 of 20, 35%). In @entity2 , methylation was present more frequently in @entity1149 (18 of 42, 43%) and cell lines (15 of 30, 50%) than in @entity151 cell lines (6 of 30, 20%; P = 0.03). Only the methylated or unmethylated forms of the gene were present in most (73 of 80, 91%) @entity5 cell lines. @entity20197 expression was present in 24 of 30 (80%) of nonmethylated @entity5 lines. All 18 methylated lines tested lacked expression irrespective of whether the unmethylated form was present, confirming biallelic inactivation in methylated lines. Gene expression was restored in all five methylated cell lines tested after treatment with the demethylating agent @entity11508 . Our results demonstrate frequent aberrant methylation of @entity20197 in @entity0 accompanied by loss of gene expression, although expression may occasionally be lost by other mechanisms.

Loss of expression and aberrant methylation of the @entity20197 (H-cadherin) gene in XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast and lung cancers', 'breast tumors'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung cancers'] @entity1149 :: ('MESH:D002289', 'Disease') :: ['non-small cell lung cancer tumors'] @entity20197 :: ('1012', 'Gene') :: ['CDH13'] @entity151 :: ('MESH:D055752', 'Disease') :: ['small cell lung cancer'] @entity11508 :: ('MESH:C014347', 'Chemical') :: ["5'-aza-2-deoxycytidine"]
@entity0 :: (MESH:D001943,Disease) :: ['breast and lung carcinomas']
