BACKGROUND: The objective of the current study was to establish a @entity35 model to investigate apoptosis in @entity479 -induced @entity2738 occurring via the Wnt/b-catenin pathway. METHODS: Male Sprague-Dawley (SD) @entity35 were randomly divided into a control group (group A), model group (group B) and @entity31179 group (group C), each consisting of 24 @entity35 , and the @entity35 were intravenously injected with @entity558 (10   g/kg body weight). After 24  h, three injections of MPS (20  mg/kg body weight) were administered intramuscularly at 24-h intervals. The @entity35 in group C were injected intramuscularly with 1   g/kg @entity31179 protein per day for 30  days, beginning at the time of the first MPS administration. The group A @entity35 were fed and housed under identical conditions but received saline injection. All animals were sacrificed at weeks 2, 4 and 8 from the first MPS injection. Histopathological staining was preformed to evaluated @entity2530 . Apoptosis was detected via quantitative @entity12809 ( @entity12809 ) @entity9342 nick-end labelling (TUNEL) staining, @entity4124 activity assay, and detection of @entity1976 and @entity2310 protein expression by immunohistochemistry and Western blotting. Wnt/b-catenin pathway signalling molecules, including activated b-catenin and @entity4569 , were detected by immunohistochemistry and Western blotting. RESULTS: Typical @entity2530 was observed in groups B and C. Apoptosis gradually increased with increasing time in both groups B and C. More severe @entity2530 and apoptosis were observed in group C compared with group B. The expression levels of @entity4124 and @entity2310 were higher while that of @entity1976 was lower in group C compared with group B. The expression levels of activated b-catenin and @entity4569 gradually decreased with increasing time in both groups B and C, and they were lower in group C compared with group B. CONCLUSIONS: The Wnt/b-catenin pathway is involved in the pathogenesis of early stage SANFH, as we have demonstrated in an SANFH @entity35 model, and it may act through the regulation of @entity4569 , which affects the cell cycle and cell apoptosis.

Apoptosis associated with Wnt/b-catenin pathway leads to XXXX -induced @entity2530 of @entity2738 .

@entity2530 :: ('MESH:D010020', 'Disease') :: ['osteonecrosis'] @entity558 :: ('MESH:C536528', 'Disease') :: ['LPS'] @entity12809 :: ('294051(Tax:10116)', 'Gene') :: ['terminal deoxynucleotidyl transferase', 'TdT'] @entity1976 :: ('24224(Tax:10116)', 'Gene') :: ['Bcl-2'] @entity4569 :: ('24577(Tax:10116)', 'Gene') :: ['c-Myc'] @entity2310 :: ('24887(Tax:10116)', 'Gene') :: ['Bax'] @entity31179 :: ('84402(Tax:10116)', 'Gene') :: ['sFRP1'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity4124 :: ('25402(Tax:10116)', 'Gene') :: ['caspase-3'] @entity2738 :: ('MESH:D005271', 'Disease') :: ['femoral head osteonecrosis'] @entity9342 :: ('MESH:C027078', 'Chemical') :: ['deoxyuridine triphosphate'] @entity479 :: ('D013256', 'Chemical') :: ['steroid']
@entity479 :: (D013256,Chemical) :: ['steroid']
