BACKGROUND: Although an increasing amount of evidence supports a "two-hit" hypothesis for the neurodevelopmental model of @entity161 , there has been no development in animal models to test this hypothesis. METHODS: An animal model was established by chronic administration of 0.1, 0.3, and 0.5mg/kg @entity3390 in P7-P21 @entity35 followed by four weeks of social isolation in childhood and then five days of social housing. Animal behaviors were measured by the open field (OF) test, the novel object recognition (NOR) test, the prepulse inhibition (PPI) test, and the elevated plus maze (EPM) test. RESULTS: We found a significant decrease in the NOR index in adolescent @entity35 compared to saline control @entity35 when administering 0.5mg/kg of @entity3390 (P=0.02). We found that social isolation had no significant effect on NOR index, though social isolation significantly increased the total distance traveled and significantly decreased the resting time in adolescent @entity35 in the OF test (P<0.001 and P=0.003, respectively). In contrast, we observed that @entity3390 administration showed no significant effects on either total distance traveled or resting time. Both @entity3390 administration and social isolation had no significant effect on the percent of PPI and @entity427 amplitudes in adolescent @entity35 . Social isolation significantly reduced the open arm entries in adolescent @entity35 in the EPM test (P=0.023), but it did not reduce the ratio to enter the open arms and the stay time in open arm. Administration of @entity3390 showed no significant effect on the indexes of entering the open arms in the EPM test on adolescent @entity35 . CONCLUSION: @entity3390 intervention in infancy is associated with the damage of long-term visual memory, whereas social isolation in childhood is associated with the increased spontaneous activity and anxiety levels. Administration of @entity3390 in infancy and social isolation in childhood are two independent factors on the @entity16 .

Establishment of a @entity161 animal model through chronic administration of XXXX in infancy and social isolation in childhood.

@entity16 :: ('MESH:D009422', 'Disease') :: ['neurodevelopmental defects'] @entity161 :: ('MESH:D012559', 'Disease') :: ['schizophrenia'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity3390 :: ('MESH:D016291', 'Chemical') :: ['MK-801'] @entity427 :: ('MESH:D012021', 'Disease') :: ['startle']
@entity3390 :: (MESH:D016291,Chemical) :: ['MK-801']
