UNASSIGNED: Oxidative stress can alter the expression level of microRNAs (miRNAs) and has a role in oxidative damage generated by reactive @entity26 species (ROS). While previous studies have demonstrated that @entity52819 , @entity34323 and @entity69643 are essential for ROS production in @entity299 , the role of these miRNAs in @entity1566 has not yet been examined. The present study focused on examining the role of @entity52819 , @entity34323 and @entity69643 during @entity5568 stimulation in @entity35 neuronal spinal cord (RN  -sc) cells. RN  -sc cells were treated with @entity5568 , and cells were harvested for reverse transcription quantitative polymerase chain reaction (RT  -qPCR) to detect the expression levels of @entity52819 , @entity34323 and @entity69643 . The results demonstrated that @entity52819 , @entity34323 and @entity69643 expression was upregulated during @entity5568 treatment. T-cell death and apoptosis were investigated using an @entity98 assay and flow cytometric analysis, respectively. Following @entity34323 silencing, @entity5568  -induced cell death and apoptosis were reduced in RN  -sc cells, while @entity69643 silencing had no effect. Furthermore, @entity39685 was identified as a direct target of @entity34323 using a Luciferase reporter assay, RT-qPCR and western blot analysis. In addition, while @entity5568 downregulated @entity39685 protein expression, this was reversed by inhibiting @entity34323 expression. Based on previous studies, it was predicted that @entity34323 has a role in ROS production through regulating @entity39685 in @entity35 spinal cord neurons.

MicroRNA  -21 regulates cell proliferation and apoptosis in XXXX  -stimulated @entity35 spinal cord neurons.

@entity5568 :: ('D006861', 'Chemical') :: ['H2O2'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity69643 :: ('100314158(Tax:10116)', 'Gene') :: ['miR  -150'] @entity39685 :: ('81516(Tax:10116)', 'Gene') :: ['Smad7'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity299 :: ('MESH:D006331', 'Disease') :: ['heart disease'] @entity1566 :: ('MESH:D013119', 'Disease') :: ['spinal cord injuries'] @entity34323 :: ('100314000(Tax:10116)', 'Gene') :: ['miR  -21'] @entity52819 :: ('100314241(Tax:10116)', 'Gene') :: ['miR  -146a']
@entity5568 :: (D006861,Chemical) :: ['H2O2']
