UNASSIGNED: @entity460 ions ( @entity141 ) play a pivotal role in cellular physiology. Often @entity141 -dependent processes are studied in commonly available cell lines. To induce @entity141 signals on demand, cells may need to be equipped with additional proteins. A prominent group of membrane proteins evoking @entity141 signals are G-protein coupled receptors (GPCRs). These proteins register external signals such as photons, odorants, and neurotransmitters and convey ligand recognition into cellular responses, one of which is @entity141 signaling. To avoid receptor cross-talk or cross-activation with introduced proteins, the repertoire of cell-endogenous receptors must be known. Here we examined the presence of @entity112 receptors in six cell lines frequently used as hosts to study cellular signaling processes. In a concentration-dependent manner, @entity112 caused a rise in intracellular @entity141 in HeLa, HEK 293, and COS-1 cells. The concentration for half-maximal activation (EC50) was in the low micromolar range. In individual cells, transient @entity141 signals and @entity141 oscillations were uncovered. The results show that (i) HeLa, HEK 293, and COS-1 cells express sufficient amounts of endogenous receptors to study cellular @entity141 signaling processes directly and (ii) these cell lines are suitable for calibrating @entity141 biosensors in situ based on @entity112 receptor evoked responses.

Molecular and functional profiling of @entity112 receptor-mediated XXXX ion signals in different cell lines.

@entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['Calcium'] @entity112 :: ('MESH:D006632', 'Chemical') :: ['histamine']
@entity460 :: (MESH:D002118,Chemical) :: ['calcium']
