Rearrangements of mitochondrial DNA in MSC16 mutant of @entity9688 ( @entity9688 .) affect mitochondrial functioning due to the alteration mainly of Complex I resulting in several metabolic changes. One-dimensional Blue-Native @entity2975 gel electrophoresis (BN-PAGE) and densitometric measurements showed that the level and in-gel capacity of Complex I were lower in MSC16 leaf and root mitochondria as compared to @entity164 ( @entity164 ). The level and capacity of supercomplex I+III(2) were always lower in leaf but not in MSC16 root mitochondria. Two-dimensional BN/ @entity214 -PAGE indicated that the band abundance for most of the subunits of Complex I was lower in MSC16 leaf and root mitochondria. Supercomplex I+III(2) level was only altered in MSC16 leaf mitochondria as measured after 2D BN/SDS-PAGE. No differences in the qualitative composition of the subunits of Complex I and supercomplex I+III(2) between MSC16 and @entity164 mitochondria were observed. In MSC16 mitochondria @entity73 could be compensated to some extent by additional respiratory chain @entity2125 dehydrogenases. A higher capacity and level of NDB-1 protein of external @entity2125 dehydrogenase was observed in MSC16 leaf and root mitochondria as compared to @entity164 . The level of COX II, mitochondrial-encoded subunit of Complex IV, was higher in MSC16 leaf and root mitochondria. However, the capacity of Complex IV was slightly higher only in MSC16 leaf mitochondria. The levels of complexes: III(2) and V and Complex V capacity did not differ in mitochondria between genotypes. An abundance of the subunits of respiratory complexes is one of the key factors determining not only their structure and functional stability but also a formation of the supercomplexes. We discuss here mitochondrial genome rearrangements in MSC16 mutant in a relation to assembly and/or stability (the lower level and capacity) of Complex I and supercomplex I+III(2).

BN-PAGE analysis of the respiratory chain complexes in mitochondria of XXXX MSC16 mutant.

@entity2125 :: ('MESH:D009243', 'Chemical') :: ['NADH'] @entity73 :: ('MESH:D003072', 'Disease') :: ['Complex I impairment'] @entity9688 :: ('3659', 'Species') :: ['cucumber', 'Cucumis sativus L'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity164 :: ('MESH:D017827', 'Disease') :: ['WT', 'wild-type'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS']
@entity9688 :: (3659,Species) :: ['cucumber']
