Endothelial cells (TEC3 cells) derived from @entity19 embryonic stem (ES) cells were used as seed cells to construct blood vessels. Tissue engineered blood vessels were made by seeding 8 106 smooth muscle cells (SMCs) obtained from @entity95 arteries onto a sheet of nonwoven @entity6886 ( @entity6987 ) fibers, which was used as a biodegradable polymer scaffold. After being cultured in DMEM medium for 7 days in vitro, SMCs grew well on the @entity6987 fibers, and the cell- @entity6987 sheet was then wrapped around a @entity6619 tube, and implanted subcutaneously into @entity19 . After 6~8 weeks, the @entity6619 tube was replaced with another @entity6619 tube in smaller diameter, and then the TEC3 cells (endothelial cells differentiated from @entity19 ES cells) were injected inside the engineered vessel tube as the test group. In the control group only culture medium was injected. Five days later, the engineered vessels were harvested for gross observation, histological and immunohistochemical analysis. The preliminary results demonstrated that the SMC- @entity6987 construct could form a tubular structure in 6~8 weeks and @entity6987 fibers were completely degraded. Histological and immunohistochemical analysis of the newly formed tissue revealed a typical blood vessel structure, including a lining of endothelial cells (ECs) on the lumimal surface and the presence of SMC and collagen in the wall. No EC lining was found in the tubes of control group. Therefore, the ECs differentiated from @entity19 ES cells can serve as seed cells for endothelium lining in tissue engineered blood vessels.

Tissue engineering of blood vessels with endothelial cells differentiated from XXXX embryonic stem cells.

@entity6987 :: ('MESH:D011454', 'Chemical') :: ['PGA'] @entity19 :: ('10090', 'Species') :: ['mouse', 'nude mice'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity6886 :: ('CHEBI:53492', 'Chemical') :: ['polyglycolic acid'] @entity6619 :: ('MESH:D012825', 'Chemical') :: ['silicon']
@entity19 :: (10090,Species) :: ['mouse']
