OBJECTIVES: The present study was designed to investigate the microbial profiles of teeth in different locations in mixed-dentition-stage @entity1 , and to compare the microbiomes of permanent and deciduous teeth in the same healthy oral cavity. METHODS: @entity527 samples of teeth in various locations-the first permanent molars, deciduous molars, deciduous @entity235 and incisors and permanent incisors-were collected from 20 healthy mixed-dentition-stage @entity1 with 10-12 permanent teeth erupted. Plaque DNA was extracted, and the V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified and subjected to sequencing. RESULTS: On average, 18,051 high-quality sequences per sample were generated. Permanent tooth sites tended to host more diverse bacterial communities than those of deciduous tooth sites. A total of 12 phyla, 21 classes, 38 orders, 66 families, 74 genera were detected ultimately. Five predominant phyla (Proteobacteria, Firmicutes, Bacteroidetes, Fusobacteria and Actinobacteria) were highly variable among sites. Of 26 genera with a mean relative abundance of >0.1%, 16 showed significant differences in relative abundance among the groups. More than 20% of the total operational taxonomical units were detected only in permanent or deciduous teeth. The variation in the microbial community composition was due mainly to permanent teeth being enriched in Actinomyces and deciduous teeth in @entity16642 . The core microbiome of @entity527 in mixed dentition comprised 19 genera with complex correlationships. CONCLUSION: Our results suggest differences in microbial diversity and composition between permanent and deciduous teeth sites in mixed dentition. Moreover, the core microbiome of these sites was determined. These findings enhance our understanding of the development of the native oral microbiota with age.

Supragingival Microbial Profiles of Permanent and Deciduous Teeth in XXXX with Mixed Dentition.

@entity1 :: ('9606', 'Species') :: ['children'] @entity16642 :: ('MESH:C531782', 'Disease') :: ['Treponema'] @entity527 :: ('MESH:D058225', 'Disease') :: ['supragingival plaque', 'Supragingival plaque'] @entity235 :: ('9615', 'Species') :: ['canines']
@entity1 :: (9606,Species) :: ['Children']
