OBJECTIVE: To evaluate the nuclear DNA content of @entity75 in liquid-based cytologic specimens prepared for static cytometry. STUDY DESIGN: The DNA content of @entity75 was evaluated in cervical samples prepared with the Autocyte PREP liquid-based cytology system (TriPath Imaging Inc., Burlington, North Carolina, U.S.A.). A series of 47 samples stained with the Papanicolaou method ( @entity102 , n = 15; @entity3146 [ @entity3146 ] 1, n = 25; @entity3146 2, n = 5; @entity3146 3, n = 2) were collected from consecutive @entity1 enrolled in an ongoing screening study at Leonor Mendes de Barros Hospital, S  o Paulo, Brazil, in 2002. Each residual sample was processed according to the Feulgen- @entity10502 method (TriPath Imaging). Ploidy evaluation was performed using the CAS 200 image analysis system and Quantitative DNA Measurement software 3.0 (version 8.1) (Becton Dickinson, San Jose, Califoria, U.S.A.). Cellular ploidy was analyzed from atypical nuclei, and the DNA index was obtained using histograms for interpretation. RESULTS: All @entity102 cases were diploid. Of the @entity3146 1 cases, 44% were diploid, 12% tetraploid, 32% @entity2007 and 12% polyploid (diploid plus tetraploid). @entity3146 2 lesions were diploid in 60% and @entity2007 in 40% of cases, whereas all @entity3146 3 lesions (100%) were @entity2007 . CONCLUSION: The liquid-based cytologic samples proved to be suitable and highly useful for DNA analysis by image cytometry, which was capable of discriminating @entity3146 3 lesions from @entity3146 1 and 2 but not @entity3146 1 from 2 lesions. @entity2007 was closely associated with @entity3146 3 lesions.

Applicability of liquid-based cytology to the assessment of DNA content in XXXX using static cytometry.

@entity1 :: ('9606', 'Species') :: ['women'] @entity2007 :: ('MESH:D000782', 'Disease') :: ['Aneuploidy', 'aneuploid'] @entity75 :: ('MESH:D001927', 'Disease') :: ['cervical lesions'] @entity102 :: ('MESH:D002908', 'Disease') :: ['chronic cervicitis'] @entity10502 :: ('CHEBI:52295', 'Chemical') :: ['thionin'] @entity3146 :: ('MESH:D018290', 'Disease') :: ['cervical intraepithelial neoplasia', 'CIN']
@entity75 :: (MESH:D001927,Disease) :: ['cervical lesions']
