BACKGROUND: @entity1468 is a complication of pregnancy associated with activation of coagulation. It is caused by the placenta, which sheds increased amounts of syncytiotrophoblast microvesicles (STBM) into the maternal circulation. We hypothesized that STBM could contribute to the haemostatic activation observed in @entity1468 . METHODOLOGY/PRINCIPAL FINDINGS: STBM were collected by perfusion of the maternal side of placentae from healthy pregnant @entity1 and @entity1 with @entity1468 at caesarean section. Calibrated automated thrombography was used to assess @entity556 generation triggered by STBM-borne tissue factor in platelet poor plasma (PPP). No @entity556 was detected in PPP alone but the addition of STBM initiated @entity556 generation in 14/16 cases. @entity1468 STBM significantly shortened the lag time (LagT, P=0.01) and time to peak @entity556 generation ( @entity2106 , P=0.005) when compared to normal STBM. Blockade of tissue factor eliminated @entity556 generation, while inhibition of tissue factor pathway inhibitor significantly shortened LagT (p=0.01) and @entity2106 (P<0.0001), with a concomitant increase in endogenous @entity556 potential. CONCLUSIONS/SIGNIFICANCE: STBM triggered @entity556 generation in normal plasma in a tissue factor dependent manner, indicating that TF activity is expressed by STBM. This is more pronounced in STBM shed from @entity1468 placentae. As more STBM are shed in @entity1468 these observations give insight into the disordered haemostasis observed in this condition.

Syncytiotrophoblast microvesicles released from XXXX placentae exhibit increased tissue factor activity.

@entity1 :: ('9606', 'Species') :: ['women'] @entity1468 :: ('MESH:D011225', 'Disease') :: ['pre-eclampsia', 'Pre-eclampsia'] @entity556 :: ('2147', 'Gene') :: ['thrombin'] @entity2106 :: ('MESH:D011697', 'Disease') :: ['TTP']
@entity1468 :: (MESH:D011225,Disease) :: ['pre-eclampsia']
