To test the hypothesis that @entity2931 receptor alpha ( @entity11716 ) and @entity11717 act together to mediate the actions of @entity2931 in the ventromedial hypothalamus (VMH), we used @entity19 with single or double knockout mutations of the @entity11716 and @entity11717 genes. Ovariectomized @entity19 were implanted with @entity53 and killed 5 days later. @entity53 treatment promoted @entity1871 receptor (PR)-immunoreactivity (-ir) in the VMH of all genotypes, but was maximal in brains of wild-type and ERbetaKO females. Analysis of specific VMH subregions revealed that PR-ir induction was limited to the caudal VMH in ERalphaKO and ERalphabetaKO @entity19 . In the rostral VMH, @entity53 only induced PR-ir in wild-type and ERbetaKO @entity19 , and the number of PR-ir neurones in this region was greater in ERbetaKO than wild-type females. Next, we tested the ability of a @entity935 agonist and @entity1420 to facilitate @entity458 in females lacking functional @entity11716 , @entity11717 , or both receptors. Ovariectomized @entity19 were implanted with @entity53 , and tested for @entity458 three times after administration of the @entity935 agonist, @entity3826 , followed by two tests concurrent with @entity1420 treatment and a final test with just @entity3826 treatment. ERalphaKO and ERalphabetaKO females failed to display @entity1515 under any testing conditions, while ERbetaKO females exhibited @entity1515 equal to that of wild-type females. Our data show that a subpopulation of PR-ir neurones is induced by @entity53 in the caudal VMH of female @entity19 lacking both @entity11716 and @entity11717 genes. We hypothesize that this action of @entity53 is either mediated by a novel ER or by the mutant portion of the @entity6803 subregion of the @entity11716 gene present in ERalphaKO brain. However, despite the presence of PR in VMH, females lacking a functional @entity11716 gene do not display @entity458 , via either ligand-dependent or -independent activation.

Double @entity2931 receptor alpha and beta knockout @entity19 reveal differences in neural @entity2931 -mediated progestin receptor induction and female XXXX .

@entity1420 :: ('MESH:D011374', 'Chemical') :: ['progesterone'] @entity2931 :: ('CHEBI:50114', 'Chemical') :: ['oestrogen'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity935 :: ('MESH:D004298', 'Chemical') :: ['dopamine'] @entity11716 :: ('13982(Tax:10090)', 'Gene') :: ['ERalpha'] @entity458 :: ('MESH:D020018', 'Disease') :: ['sexual behaviour'] @entity53 :: ('MESH:D004958', 'Chemical') :: ['oestradiol', '17beta-oestradiol', 'Oestradiol'] @entity6803 :: ('MESH:D005668', 'Chemical') :: ['AF2'] @entity1871 :: ('CHEBI:59826', 'Chemical') :: ['progestin'] @entity11717 :: ('13983(Tax:10090)', 'Gene') :: ['ERbeta'] @entity3826 :: ('MESH:D001058', 'Chemical') :: ['apomorphine'] @entity1515 :: ('MESH:D008141', 'Disease') :: ['lordosis', 'lordosis behaviour']
@entity458 :: (MESH:D020018,Disease) :: ['sexual behaviour']
