AIMS: MR-guided High Intensity Focused Ultrasound (MR-HIFU) ablation and Radiofrequency Ablation (RFA) are under research as possible substitutes for surgery in @entity0 @entity1 . The histopathologic appearance of ablated tissue has not been studied in great detail. This study aimed to compare histopathologic features of @entity0 after MR-HIFU ablation and RFA. METHODS: MR-HIFU ablation and RFA were performed in and ex vivo. @entity5 in six mastectomy specimens were partially ablated with RFA or MR-HIFU. In vivo MR-HIFU ablation was performed three to six days before excision, RFA was performed in the operation room. Tissue was fixed in @entity406 and processed to @entity1710 and @entity1711 (H_E) and @entity10585 ( @entity10585 ) stained slides. Morphology and cell viability were assessed. RESULTS: Ex vivo ablation resulted in clear morphologic changes after RFA versus subtle differences after MR-HIFU. @entity10585 staining was decreased or absent. H_E tended to underestimate the size of thermal damage. In vivo MR-HIFU resulted in @entity39 -like changes. Surprisingly, some ablated lesions were @entity10585 positive. Histopathology after in vivo RFA resembled ex vivo RFA, with @entity1712 and elongated nuclei. Lesion borders were sharp after MR-HIFU and indistinct after RFA. CONCLUSION: Histopathologic differences between MR-HIFU ablated tissue and RF ablated tissue were demonstrated. @entity10585 was more reliable for cell viability assessment than H_E when used directly after ablation, while H_E was more reliable in ablated tissue left in situ for a few days. Our results contribute to improved understanding of histopathologic features in @entity0 treated with minimally invasive ablative techniques. This article is protected by copyright. All rights reserved.

Histopathology of XXXX after magnetic resonance-guided high intensity focused ultrasound and radiofrequency ablation.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity1712 :: ('MESH:D004802', 'Disease') :: ['hyper-eosinophilic stroma'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer', 'breast cancer lesions'] @entity10585 :: ('3856', 'Gene') :: ['CK8', 'cytokeratin 8'] @entity5 :: ('MESH:D009369', 'Disease') :: ['Tumours'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrotic'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity406 :: ('MESH:D005557', 'Chemical') :: ['formalin'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hematoxylin']
@entity0 :: (MESH:D001943,Disease) :: ['breast cancer']
