Interleukin-10 ( @entity688 ) is a pleiotropic cytokine and plays an important role in @entity32 , immunoregulation and the pathogenesis of @entity66 . Therefore, it is our interest to isolate, clone, sequence and characterize @entity688 gene from the fish @entity32342 (Lr). The gene was amplified using genomic DNA isolated from head kidney with primers designed on conserved sequence homologues of fishes belonging to Cyprinidae family. The gLrIL-10 is 1467 nucleotides long with five exons and four introns sharing the same organization as of @entity1 @entity688 genes. An open reading frame of 537 bp was found to encode a putative 179 @entity535 protein with a signal peptide of 22 @entity1522 with conserved signature sequence motif. Sequence analysis showed similarity with the @entity688 from most fresh water fishes of Cyprinidae family. LrIL-10 has 27.2 % identity and 54.95 % similarity with the @entity1 @entity688 . Sequence analysis followed by phylogenetic studies showed highest identity with @entity67990 (98%) followed by @entity7136 (93%), @entity68541 (89%) and is distantly related to @entity1 , @entity2608 and frog. These data from primary sequence characterization may be used to further understand transcriptional regulation and functional characterization of LrIL-10 in relation to species-specific molecular immunology. ABBREVIATIONS: @entity688 - Interleukin-10, Lr - @entity32342 , nt - @entity2036 .

Genomic cloning and sequence analysis of Interleukin-10 from XXXX .

@entity1 :: ('9606', 'Species') :: ['mammalian', 'human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity2608 :: ('9544', 'Species') :: ['rhesus monkey'] @entity66 :: ('MESH:D004194', 'Disease') :: ['various diseases'] @entity2036 :: ('MESH:D009711', 'Chemical') :: ['nucleotides'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity67990 :: ('72446', 'Species') :: ['Catla catla'] @entity7136 :: ('7962', 'Species') :: ['Cyprinus carpio'] @entity688 :: ('3586', 'Gene') :: ['IL-10'] @entity68541 :: ('13095', 'Species') :: ['Hypophthalmichthys molitrix'] @entity32342 :: ('84645', 'Species') :: ['Labeo rohita']
@entity32342 :: (84645,Species) :: ['Labeo rohita']
