@entity2 cells express several cytokeratins (CKs) that are subdivided into type I ( @entity22067 -23) and type II ( @entity19488 -8) subclasses. The functions of CKs in @entity2 cells have not been fully elucidated. The purpose of this study was to investigate the role of CKs in the invasion of @entity2 cells. We investigated the expression levels of @entity5822 , 8, 18 and 19 in 12 @entity1149 ( @entity1149 ) and seven @entity4153 cell lines by quantitative immunoblotting. The expression levels of these four CKs were significantly higher in the @entity1149 cells. The @entity1149 cell line HI1017 expressed @entity10585 and 18; A549 cells expressed @entity5822 , 8, 18 and 19, respectively. Invasive sublines of HI1017 and A549 were established by repeated selection of invasive cells using a membrane invasion chamber system. The invasive cell lines showed lower expression levels of CKs compared with the parental cells. Exogenous @entity4166 also resulted in a decrease in invasiveness of the HI1017 cells. Suppression of either @entity10585 or @entity2873 by short interfering RNAs led to a decrease in the total CKs and increased invasiveness of both the HI1017 and A549 cells. A549 cells expressed very low levels of @entity4166 . Suppression of @entity4166 affected neither invasive ability nor total CK amount in the A549 cells. Our observations indicate that CK expression levels were inversely associated with invasiveness of the @entity1149 cell lines, and suggest that expression levels of dominant CKs may affect invasive ability.

Cytokeratins negatively regulate the invasive potential of XXXX cell lines.

@entity22067 :: ('3857', 'Gene') :: ['CK9'] @entity4166 :: ('3880', 'Gene') :: ['CK19'] @entity4153 :: ('MESH:D018288', 'Disease') :: ['SCLC'] @entity10585 :: ('3856', 'Gene') :: ['CK8'] @entity1149 :: ('MESH:D002289', 'Disease') :: ['NSCLC', 'non-small cell lung cancer'] @entity2 :: ('MESH:D008175', 'Disease') :: ['Lung cancer', 'lung cancer'] @entity2873 :: ('3875', 'Gene') :: ['CK18'] @entity5822 :: ('3855', 'Gene') :: ['CK7'] @entity19488 :: ('3848', 'Gene') :: ['CK1']
@entity2 :: (MESH:D008175,Disease) :: ['lung cancer']
