@entity375 cells mate by an inducible pathway that involves agglutination, mating projection formation, cell fusion, and nuclear fusion. To obtain insight into the mating differentiation of @entity375 , we carried out a large-scale transposon tagging screen to identify genes whose expression is regulated by mating pheromone. 91,200 transformants containing random lacZ insertions were screened for @entity7791 ( @entity7791 ) expression in the presence and absence of alpha factor, and 189 strains containing @entity5681 -regulated lacZ insertions were identified. Transposon insertion alleles corresponding to 20 genes that are novel or had not previously been known to be pheromone regulated were examined for effects on the mating process. Mutations in four novel genes, FIG1, FIG2, @entity70188 / FIG3, and FIG4 were found to cause mating defects. Three of the proteins encoded by these genes, Fig1p, Fig2p, and Fig4p, are dispensible for cell polarization in uniform concentrations of mating pheromone, but are required for normal cell polarization in mating mixtures, conditions that involve cell-cell communication. Fig1p and Fig2p are also important for cell fusion and conjugation bridge shape, respectively. The fourth protein, @entity70188 /Fig3p, is required for nuclear fusion. Fig1p and Fig2p are likely to act at the cell surface as Fig1:: @entity7791 and Fig2:: @entity7791 fusion proteins localize to the periphery of mating cells. Fig4p is a member of a family of eukaryotic proteins that contain a domain homologous to the @entity375 @entity38560 . Our results indicate that a variety of novel genes are expressed specifically during mating differentiation to mediate proper cell morphogenesis, cell fusion, and other steps of the mating process.

Pheromone-regulated genes required for XXXX mating differentiation.

@entity7791 :: ('MESH:D016537', 'Disease') :: ['beta-gal', 'beta-galactosidase'] @entity38560 :: ('853668(Tax:4932)', 'Gene') :: ['Sac1p'] @entity70188 :: ('855087(Tax:4932)', 'Gene') :: ['Kar5p', 'KAR5'] @entity375 :: ('4932', 'Species') :: ['yeast', 'Saccharomyces cerevisiae', 'Yeast'] @entity5681 :: ('CHEBI:26013', 'Chemical') :: ['pheromone']
@entity375 :: (4932,Species) :: ['yeast']
