BACKGROUND: A strong antimicrobial synergism between @entity1216 (CHX) and @entity273 ( @entity274 ) has been reported, but there is not enough data on the @entity137 of this combination. The primary aim of this study was to evaluate the @entity137 of CHX- @entity274 combination in different concentrations and secondary aim is to assess the influence of @entity274 on @entity137 of CHX on cultured @entity1 periodontal ligament (PDL) fibroblasts. MATERIALS AND METHODS: The PDL cells were cultured from healthy @entity1 third molar teeth and were exposed to six prepared solutions (0.2% and 2% CHX separately and in combination with 1% and 3% @entity274 ). The @entity98 assay was applied to assess their effects on the viability of the PDL cells. Two-way analysis of variance approach and subgroup analysis was performed to evaluate the differences in mean cell viability values. A level of P < 0.05 was considered as statistically significant. RESULTS: All tested solutions were toxic to PDL cells. There was a significant interaction effect between CHX and @entity274 . The 2% CHX combined with 3% @entity274 was the most and 0.2% CHX was the least cytotoxic solutions. The 2% CHX was significantly more toxic than 0.2% CHX and @entity274 combinations. The @entity137 of 0.2% CHX and @entity274 combinations did not significantly rise by increasing the concentration of @entity274 from 1% to 3%. CONCLUSION: @entity274 affected the @entity137 of CHX in a variable concentration-dependent manner. Based on the results of this study, it can be concluded that 2% CHX alone and in combination with either 1 or 3% @entity274 are significantly more toxic than 0.2% CHX alone and in combination with 1 and 3% @entity274 . Therefore, to benefit from the synergistic antimicrobial effect between CHX and @entity274 , with a minimal @entity137 , it is recommended to use 0.2% concentration of CHX combined with 3% @entity274 .

@entity137 of @entity1216 - XXXX combination in different concentrations on cultured @entity1 periodontal ligament fibroblasts.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1216 :: ('MESH:D002710', 'Chemical') :: ['chlorhexidine'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H2O2'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['hydrogen peroxide'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT']
@entity273 :: (MESH:D006861,Chemical) :: ['hydrogen peroxide']
