Micro RNAs (miRs) are involved in many biological processes. The challenge of identifying genes influenced by miRs is evidenced by the relatively few validated @entity21445 -target interactions. In this work, we used the Mus spretus SPRET/Ei strain as an in vivo system to identify new @entity21445 -target relations. Mus spretus diverged from @entity19 over one million years ago, making it genetically and phenotypically divergent. SPRET/Ei @entity19 are resistant to @entity32 and several @entity5 , making them attractive for different research fields. Their phenotype is unique and is considerably different from that of almost all other laboratory @entity19 strains. We exploited the characteristics of SPRET/Ei @entity19 as a tool to identify @entity21445 -target relationships. Hepatic genes and miRs differentially expressed between C57BL/6 and SPRET/Ei @entity19 at basal levels were identified with an Affymetrix microarray and a multiplex qPCR, respectively. A total of 955 genes and 38 miRs were identified as differentially expressed. Increased @entity21445 expression might result in downregulation of its target mRNA and vice versa. Subsequently, we used our @entity21445 and mRNA data to identify possible in vivo @entity21445 -target interactions. Ingenuity pathway analysis (IPA) analysis revealed 380 possible @entity21445 -target interactions. Five miRs were selected for experimental validation by in vivo overexpression of the miRs. This resulted in the confirmation of six previously unknown @entity21445 -target interactions: @entity41984 , @entity68546 ; @entity38408 , @entity68547 , @entity68548 , and Nrd1d2; @entity20214 , @entity68549 ; and @entity68550 , @entity68551 . In conclusion, we show that SPRET/Ei @entity19 can be used as a platform for @entity21445 -target identification in vivo, and we used this platform to identify and experimentally confirm @entity21445 -target interactions.

Determining differentially expressed miRNAs and validating miRNA-target relationships using the SPRET/Ei XXXX strain.

@entity41984 :: ('387164(Tax:10090)', 'Gene') :: ['miR-146a'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity68550 :: ('735266(Tax:10090)', 'Gene') :: ['miR-592'] @entity68547 :: ('12686(Tax:10090)', 'Gene') :: ['Elovl3'] @entity68549 :: ('100072(Tax:10090)', 'Gene') :: ['Camta1'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'Mus musculus'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancers'] @entity20214 :: ('387173(Tax:10090)', 'Gene') :: ['miR-155'] @entity21445 :: ('751557(Tax:10090)', 'Gene') :: ['miR'] @entity38408 :: ('387168(Tax:10090)', 'Gene') :: ['miR-150'] @entity68551 :: ('74051(Tax:10090)', 'Gene') :: ['Steap2'] @entity68548 :: ('16529(Tax:10090)', 'Gene') :: ['Kcnk5'] @entity68546 :: ('70546(Tax:10090)', 'Gene') :: ['Zdhhc2']
@entity19 :: (10090,Species) :: ['mouse']
