To elucidate the role of muscle glycogen storage on regulation of @entity19617 protein expression and whole-body @entity413 tolerance, muscle glycogen level was manipulated by exercise and insulin administration. Sixty @entity35 were evenly separated into three groups: control (CON), immediately after exercise (EX0), and 16 h after exercise (EX16). @entity35 from each group were further divided into two groups: saline- and insulin-injected. The 2-day exercise protocol consisted of 2 bouts of 3-h swimming with 45-min rest for each day, which effectively depleted glycogen in both red gastrocnemius (RG) and plantaris muscles. EX0 @entity35 were sacrificed immediately after the last bout of exercise on second day. CON and EX16 @entity35 were intubated with 1 g/kg @entity413 solution following exercise and recovery for 16 h before muscle tissue collection. Insulin (0.5 microU/kg) or saline was injected daily at the time when @entity413 was intubated. Insulin injection elevated muscle glycogen levels substantially in both muscles above saline-injected group at CON and EX16. With previous day insulin injection, EX0 preserved greater amount of postexercise glycogen above their saline-injected control. In the saline-injected @entity35 , EX16 significantly increased @entity19617 protein level above CON, concurrent with muscle glycogen supercompensation. Insulin injection for EX16 @entity35 significantly enhanced muscle glycogen level above their saline-injected control, but the increases in muscle @entity19617 protein and whole-body @entity413 tolerance were attenuated. In conclusion, the new finding of the study was that glycogen overload by postexercise insulin administration significantly abolished the exercise-induced increases in @entity19617 protein and @entity413 tolerance.

Glycogen overload by postexercise insulin administration abolished the exercise-induced increase in XXXX protein.

@entity35 :: ('10116', 'Species') :: ['Sprague-Dawley rats', 'Rats', 'rats'] @entity19617 :: ('25139(Tax:10116)', 'Gene') :: ['GLUT4'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity19617 :: (25139(Tax:10116),Gene) :: ['GLUT4']
