Extracellular matrix (ECM) components, through specific peptide motifs such as @entity11599 (RGD), interact with integrins and can modify the behavior of cells. @entity2533 ( @entity2533 ) is the main cytokine involved in the synthesis of ECM proteins. We analyzed the effect of a RGD-containing peptide, as @entity9044 (RGDS), on the regulation of @entity2533 secretion in cultured @entity1 mesangial cells. We found that RGDS increased mRNA expression and secretion of @entity2533 by stimulating the @entity2533 gene promoter. This effect was dependent on the interaction of RGDS with integrins. We evaluated the signaling pathways implicated in @entity2533 production by analyzing the effect of RGDS on kinase-related integrins. RGDS stimulated @entity669 phosphorylation as well as @entity12019 ( @entity12019 ) activity. However, @entity669 kinase inhibitors did not prevent the RGDS effect. In contrast, the inhibition of @entity12019 by cell transfection with a kinase dead- @entity12019 completely abolished the increased @entity2533 secretion and promoter activity in the presence of RGDS. Thus RGDS modulates the secretion of @entity2533 , probably through increased synthesis by interacting with integrins and activating @entity12019 . This supports a role for ECM components in the regulation of their own secretion.

XXXX (RGDS) peptide stimulates transforming growth factor beta1 transcription and secretion through integrin activation.

@entity1 :: ('9606', 'Species') :: ['human'] @entity12019 :: ('3611', 'Gene') :: ['ILK', 'integrin-linked kinase'] @entity2533 :: ('7040', 'Gene') :: ['TGF-beta1', 'Transforming growth factor-beta1'] @entity11599 :: ('MESH:C047981', 'Chemical') :: ['Arg-Gly-Asp'] @entity9044 :: ('MESH:C046617', 'Chemical') :: ['Arg-Gly-Asp-Ser'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine']
@entity9044 :: (MESH:C046617,Chemical) :: ['Arg-Gly-Asp-Ser']
