UNASSIGNED: @entity261 ( @entity261 ) arises most commonly from parathyroid (PT) gland damage associated with neck surgery, and is typically treated with oral @entity460 and active @entity560 . Such treatment effectively increases levels of serum @entity460 (sCa), but also brings risk of @entity5200 and @entity8 . There is thus considerable interest in using @entity10173 or @entity10173 analogs to treat @entity261 . To facilitate study of this disease and the assessment of new treatment options, we developed two @entity19 models of acquired @entity261 , and used them to assess efficacy of @entity10173 (1-34) as well as a long-acting @entity10173 analog (LA- @entity10173 ) in regulating blood @entity460 levels. In one model, we used PTHcre-iDTR @entity19 in which the @entity20829 ( @entity20829 ) is selectively expressed in PT glands, such that systemic DT administration selectively ablates parathyroid cells. For the second model, we generated GFP-PT @entity19 in which green fluorescent protein (GFP) is selectively expressed in PT cells, such that parathyroidectomy ( @entity4045 ) is facilitated by green fluorescence of the PT glands. In the PTHcre-iDTR @entity19 , DT injection (2   *   5    g/kg, i.p.) resulted in moderate yet consistent reductions in serum @entity10173 and sCa levels. The more severe @entity261 phenotype was observed in GFP-PT @entity19 following GFP-guided PTX surgery. In each model, a single subcutaneous injection of LA- @entity10173 increased sCa levels more effectively and for a longer duration (>24 hours) than did a 10-fold higher dose of @entity10173 (1-34), without causing excessive urinary @entity460 excretion. These new @entity19 models thus faithfully replicate two degrees of acquired @entity261 , moderate and severe, and may be useful for assessing potential new modes of therapy.    2015 American Society for Bone and Mineral Research.

Diphtheria Toxin- and GFP-Based @entity19 Models of Acquired XXXX and Treatment With a Long-Acting Parathyroid Hormone Analog.

@entity8 :: ('MESH:D007674', 'Disease') :: ['renal damage'] @entity10173 :: ('19226(Tax:10090)', 'Gene') :: ['PTH'] @entity20829 :: ('15200(Tax:10090)', 'Gene') :: ['DTR', 'diphtheria toxin (DT) receptor'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity4045 :: ('D010852', 'Chemical') :: ['PTX'] @entity261 :: ('MESH:D007011', 'Disease') :: ['hypoparathyroid', 'Hypoparathyroidism', 'HP'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity5200 :: ('MESH:D053565', 'Disease') :: ['hypercalciuria'] @entity560 :: ('MESH:D014807', 'Chemical') :: ['vitamin D']
@entity261 :: (MESH:D007011,Disease) :: ['Hypoparathyroidism']
