BACKGROUND: Meconopsis horridula @entity68512 (M. horridula) has been used as a traditional Tibetan medicine to relieve heat and @entity158 as well as mobilize static blood, and it is recognized as a good treatment for @entity2981 . This study is the first trial to evaluate the @entity5 inhibitory activity of M. horridula extract and its underlying mechanism in the hope of providing evidence to support the anticancer function of M. horridula. METHODS AND RESULTS: M. horridula extract was cytotoxic to L1210 cells in a dose- and time-dependent manner. @entity4653 ( @entity4653 ) observation revealed obvious morphological changes in L1210 cells after M. horridula treatment. Flow cytometry analysis demonstrated that the extract dose-dependently induced early apoptosis. Additional apoptosis parameters, such as alterations in nuclear morphology and DNA damage, were also observed. Furthermore, M. horridula treatment induced G2/M arrest. M. horridula treatment significantly increased reactive @entity26 species (ROS) production, suggesting that ROS are a key factor in M. horridula-induced apoptosis. Volatile constituent detection found 15 abundant chemicals in M. horridula, which may contribute to its anticancer effect. CONCLUSION: In conclusion, M. horridula extract induced L1210 cell apoptosis and inhibited proliferation through G2/M phase arrest, and ROS were involved in the process.

The Antitumor Activity of Meconopsis Horridula XXXX , a Traditional Tibetan Medical Plant, in Murine Leukemia L1210 Cells.

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity158 :: ('MESH:D010146', 'Disease') :: ['pain'] @entity68512 :: ('109541(Tax:10090)', 'Gene') :: ['Hook'] @entity2981 :: ('MESH:D003288', 'Disease') :: ['bruises'] @entity4653 :: ('MESH:D004401', 'Disease') :: ['scanning electron microscope', 'SEM']
@entity68512 :: (109541(Tax:10090),Gene) :: ['Hook']
