In insect gap junctions, species-specific differences occur in response to the purported gap junction uncoupling agent, @entity32215 . Changes in gap junctional communication between oocytes and their epithelial cells following treatment with @entity32215 were assayed in Oncopeltus fasciatus (the milkweed bug), Hyalophora cecropia (the American @entity12638 ), and @entity238 . In all three species, microinjection of untreated control follicles with @entity6890 revealed extensive dye coupling among epithelial cells and between epithelial cells and their oocytes. Also for all three species, treatment with @entity10333 appeared to completely block dye coupling and increase oocyte input resistance. The effect on electrical coupling varied. In @entity238 , @entity10333 diminished the electrical coupling from 64% (0.64 coupling coefficient) in controls to 53% in treated follicles. In Hyalophora, the coupling ratio remained the same following treatment. In Oncopeltus, @entity10333 actually increased the electrical coupling ratio from 84% in controls to 94% in treated follicles. While 0.5 mM @entity10333 left some Oncopeltus epithelial cells dye coupled to the oocyte, the electrical coupling ratio was increased slightly more by this concentration than by 1 or 5 mM @entity10333 solutions, although the differences were not significant. While input resistance (R(o )) increased in all three following treatment with @entity10333 , there was considerable difference in the magnitude of the response. Average oocyte R(o ) for Oncopeltus increased the least of the three species, rising from 196-240 kOhm. Both Hyalophora, with a nearly fourfold increase from 230-900 kOhm or more, and @entity238 , with a twofold increase from 701 kOhm to over 1.2 MegOhm showed much larger changes. Results shown here indicate that insect gap junctions have more varied responses to this common gap junction antagonist than have been reported for their vertebrate counterparts. Arch.

Varied effects of @entity32215 on gap junctional communication between ovarian epithelial cells and oocytes of Oncopeltus fasciatus, Hyalophora cecropia, and XXXX .

@entity12638 :: ('7091', 'Species') :: ['silk moth'] @entity10333 :: ('CHEBI:37868', 'Chemical') :: ['octanol'] @entity32215 :: ('MESH:D020003', 'Chemical') :: ['1-octanol'] @entity6890 :: ('MESH:C017475', 'Chemical') :: ['Lucifer yellow CH'] @entity238 :: ('7227', 'Species') :: ['Drosophila melanogaster', 'Drosophila']
@entity238 :: (7227,Species) :: ['Drosophila melanogaster']
