Components of the bacterial @entity7954 ( @entity7955 ) : @entity1735 phosphortransferase system (PTS) have multiple regulatory roles in addition to PEP-dependent transport/phosphorylation of numerous @entity3363 . We have recently shown that, in an opportunistic @entity1 pathogen, @entity5345 , enzyme IIA(Glc) (EIIA(Glc)) interacts with a peptidase that has high sequence similarity to @entity1 insulin-degrading enzymes, called Vibrio insulin-degrading enzyme (vIDE). Although the vIDE-EIIA(Glc) interaction is independent of the phosphorylation state of EIIA(Glc), vIDE shows no peptidase activity unless complexed with the unphosphorylated form of EIIA(Glc). A deletion mutant of ideV, the gene encoding vIDE, shows remarkably lower degrees of survival and virulence than the wild-type strain in @entity19 , implying that vIDE is a virulence factor. In this study, we investigated regulation of ideV expression at the transcriptional level. Primer extension analysis identified two different transcriptional start sites of ideV: P(L) for the longer transcript and P(S) for the shorter transcript. We performed ligand fishing experiments by using the promoter region of ideV and found that the @entity268 receptor protein (CRP) specifically binds to the promoter. DNase I footprinting experiments revealed that CRP binds to a region between the two promoters. In vitro transcription assays showed that CRP activates ideV P(S) transcription in the presence of @entity268 whose concentration is regulated by EIIA(Glc). These results suggest that EIIA(Glc) regulates the expression level of vIDE as well as its activity.

Expression of XXXX insulin-degrading enzyme is regulated by the @entity268 -CRP complex.

@entity1 :: ('9606', 'Species') :: ['mammalian', 'human'] @entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity7955 :: ('MESH:C073248', 'Chemical') :: ['PEP'] @entity7954 :: ('MESH:D010728', 'Chemical') :: ['phosphoenolpyruvate'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['carbohydrate'] @entity5345 :: ('672', 'Species') :: ['Vibrio vulnificus'] @entity3363 :: ('MESH:D002241', 'Chemical') :: ['carbohydrates']
@entity5345 :: (672,Species) :: ['Vibrio vulnificus']
