OBJECTIVE: To investigate the effect of in vitro fertilization and embryo transfer (IVF-ET) and @entity74 ( @entity74 ) for the couples at risk of having @entity1 with @entity8674 . METHODS: Four couples carrying different @entity2395 mutations received standard IVF treatment. Embryo biopsy was conducted. Single blastomeres were genotyped by a protocol involving primer extension preamplification, nested polymerase chain reaction and reverse dot-blot analysis. Only the unaffected embryos were transferred to the uterus. RESULTS: A total of 97 oocytes were retrieved from the four female carriers. Among them, 83% showed two pronuclei. Embryo biopsy was performed on 47 of these embryos. The amplification efficiency was 84.8%. The average ADO rate was 14.9%. Ten unaffected embryos were transferred. A twin pregnancy with one blighted ovum was confirmed at 7 weeks' gestation by ultrasonography and one normal baby and one carrier of @entity2395 mutation were born finally. CONCLUSION: This unaffected pregnancy resulting from @entity74 for @entity8674 demonstrates that @entity74 technique can be a powerful diagnostic tool for couples carrying @entity8674 mutations who desire a healthy @entity1 and wish to avoid abortion of an affected fetus.

[Preimplantation genetic diagnosis for XXXX ].

@entity1 :: ('9606', 'Species') :: ['children', 'child'] @entity8674 :: ('MESH:D017086', 'Disease') :: ['beta-thalassemia'] @entity74 :: ('MESH:D030342', 'Disease') :: ['PGD', 'preimplantation genetic diagnosis'] @entity2395 :: ('MESH:D013789', 'Disease') :: ['thalassemia']
@entity8674 :: (MESH:D017086,Disease) :: ['beta-thalassemia']
