The intestinal transplantation procedure obligates an early functional deficit in intestinal grafts. Graft irradiation has been used to modulate post-transplant immune reactions; however, irradiation may cause further deterioration of the function of the transplanted intestine. Using the @entity235 model, we investigated the influence of the transplant procedures, with and without ex vivo graft irradiation, on early intestinal graft function and histopathology. Outbred hound @entity235 underwent autointestinal transplantation with (n=8) or without (n=5) 7.5 Gy ex vivo graft irradiation. Mucosal @entity40162 and P-glycoprotein, routine immunohistopathology, and intestinal absorptive function were studied. @entity1017 was slow after surgery, but was comparable in the irradiated and nonirradiated groups. During the early post-transplant period, both groups showed defects in intestinal absorption, associated with decreased @entity40162 3A4 activity and reduced P-glycoprotein expression, regardless of graft irradiation. These changes returned to normal in both groups by day 28. Histopathologically, epithelial apoptosis showed a slight increase 1 hour after transplantation; however, there was no evidence of @entity854 including arterial changes associated with irradiation. In addition, the frequency of T and B lymphocytes in the lamina propria were not significantly influenced by the transplant surgery or ex vivo irradiation. Thus, early after transplantation, intestinal function was impaired and effectiveness of orally administered immunosuppressive drugs was significantly altered. Graft irradiation did not induce further defects in intestinal function or cause @entity854 .

Morphology and function of XXXX small intestinal autografts: with particular interest in the influence of ex vivo graft irradiation.

@entity235 :: ('9615', 'Species') :: ['canine', 'dogs'] @entity854 :: ('MESH:D018376', 'Disease') :: ['histopathologic abnormalities'] @entity40162 :: ('487648(Tax:9615)', 'Gene') :: ['cytochrome P450'] @entity1017 :: ('MESH:D015430', 'Disease') :: ['Weight gain']
@entity235 :: (9615,Species) :: ['canine']
