@entity21215 mutant Rc29, with a premature stop codon mutation in the m29 open reading frame (ORF), produced no apparent phenotype in cell culture or following infection of BALB/c @entity19 . In contrast, a similar mutant virus, Rc29.1, with a premature stop codon mutation in its m29.1 ORF, showed reduced virus yields (2-3 log(10) p.f.u. ml(-1)) in tissue culture. Mutant virus yields in BALB/c @entity19 were delayed, reduced ( approximately 1 log(10) p.f.u. per tissue) and persisted less well in salivary glands compared with wild-type (wt) and revertant (Rv29.1) virus. In severe combined @entity465 @entity19 , Rc29.1 virus showed delayed and reduced replication initially in all tissues (liver, spleen, kidneys, heart, lung and salivary glands). This delayed death until 31 days post-infection (p.i.) compared with wt (23 days p.i.) but at death virus yields were similar to wt. m29 gene transcription was initiated at early times post-infection, while production of a transcript from ORF m29.1 in the presence of @entity1880 indicated that it was an immediate-early gene. ORFs m29.1 and M28 are expressed from a bicistronic message, which is spliced infrequently. However, it is likely that each ORF expresses its own protein, as antiserum derived in @entity95 to the m29.1 protein expressed in bacteria from the m29.1 ORF detected only one protein in Western blot analysis of the size predicted for the m29.1 protein. Our results suggest that neither ORF is essential for virus replication but m29.1 is important for optimal viral growth in vitro and in vivo.

XXXX open reading frame m29.1 augments virus replication both in vitro and in vivo.

@entity19 :: ('10090', 'Species') :: ['mice'] @entity1880 :: ('MESH:D003513', 'Chemical') :: ['cycloheximide'] @entity21215 :: ('10366', 'Species') :: ['Murine cytomegalovirus'] @entity95 :: ('9986', 'Species') :: ['rabbits'] @entity465 :: ('MESH:D007153', 'Disease') :: ['immunodeficiency']
@entity21215 :: (10366,Species) :: ['Murine cytomegalovirus']
