The expression of oviduct-specific recombinant proteins in transgenic @entity1573 is a promising technology for the production of therapeutic biologics in eggs. In this study, we constructed a lentiviral vector encoding an expression cassette for @entity1 neutrophil defensin 4 ( @entity69918 ), a compound that displays high activity against @entity526 , and produced transgenic @entity1573 that expressed the recombinant @entity69918 protein in egg whites. After the antimicrobial activity of the recombinant @entity69918 protein was tested at the cellular level, a 2.8-kb ovalbumin promoter was used to drive @entity69918 expression specifically in oviduct tissues. From 669 injected eggs, 218 @entity1573 were successfully hatched. Ten G0 roosters, with semens identified as positive for the transgene, were mated with wild-type hens to generate G1 @entity1573 . From 1,274 total offspring, fifteen G1 transgenic @entity1573 were positive for the transgene, which was confirmed by PCR and Southern blotting. The results of the Southern blotting and genome walking indicated that a single copy of the @entity69918 gene was integrated into chromosomes 1, 2, 3, 4, 6 and 24 of the @entity1573 . As expected, @entity69918 expression was restricted to the oviduct tissues, and the levels of both transcriptional and translational @entity69918 expression varied greatly in transgenic @entity1573 with different transgene insertion sites. The amount of @entity69918 protein expressed in the eggs of G1 and G2 heterozygous transgenic @entity1573 ranged from 1.65  g/ml to 10.18  g/ml. These results indicated that the production of transgenic @entity1573 that expressed @entity69918 protein in egg whites was successful.

Oviduct-specific expression of XXXX neutrophil defensin 4 in lentivirally generated transgenic @entity1573 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity1573 :: ('9031', 'Species') :: ['chickens'] @entity69918 :: ('1669', 'Gene') :: ['HNP4'] @entity526 :: ('562', 'Species') :: ['Escherichia coli']
@entity1 :: (9606,Species) :: ['human']
