OBJECTIVE: To assess intratumor molecular heterogeneity in @entity7735 ( @entity5123 ) and to investigate if intratumor molecular heterogeneity is associated with cell proliferation or apoptotic indexes. STUDY DESIGN: Nine @entity406 -fixed paraffin-embedded samples of @entity5123 of salivary glands were included in the study. Cell proliferation was estimated by using Ki-67 immunohistochemistry, and apoptotic index was achieved by combining terminal deoxynucleotidyl transferase @entity1977 nick end-labeling with morphology. A minimum of two different @entity5 areas of each sample was microdissected, and DNA was extracted. DNA extracted from the @entity5 capsule was used as normal matched control. Different @entity5 areas were at least 4  mm from one another and comprised @entity5 cell-rich areas. Loss of heterozygosity was assessed by a panel of six polymorphic microsatellite markers located at chromosomes 3 (D3 S1029), 9 (D9 S162 and D9 S157), 11 (D11 S1369), and 17 (P53 and AFM238 WF2). RESULTS: Six of nine samples showed intratumor heterogeneity on the basis of the loss of heterozygosity findings. Intratumor molecular heterogeneity did not show association with cell proliferation or apoptotic indexes (P  >  .05). CONCLUSIONS: Our findings point to the existence of intratumor molecular heterogeneity in salivary gland @entity5123 . This is an advance in the efforts to clarify @entity5123 molecular pathogenesis, showing that this characteristic is not exclusive to malignant solid @entity5 .

Intratumor molecular heterogeneity in XXXX of the salivary glands.

@entity7735 :: ('MESH:C563250', 'Disease') :: ['salivary gland pleomorphic adenoma'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity406 :: ('MESH:D005557', 'Chemical') :: ['formalin'] @entity1977 :: ('CHEBI:17625', 'Chemical') :: ['dUTP'] @entity5123 :: ('MESH:D008949', 'Disease') :: ['PA']
@entity5123 :: (MESH:D008949,Disease) :: ['pleomorphic adenoma']
