Wilbrandia ebracteata is a medicinal plant from South America used in folk medicine for the treatment of chronic @entity627 . We have shown that the high performance liquid chromatography-characterized (HPLC) @entity1044 fraction isolated from Wilbrandia ebracteata (WEDC) inhibits the parameters observed in experimental models of @entity32 in vivo and in vitro. In the present study, we extend our previous observations on the analgesic effects of WEDC by investigating its actions using the hot plate test and zymosan-induced writhing test in @entity19 , as well as zymosan-induced @entity625 in @entity35 evaluating articular inflammatory @entity158 , cell migration and determination of NO release into the joint exudate. The effect of WEDC on the activity of @entity9774 and @entity4645 in vitro and its ulcerogenic capacity in vivo were also investigated. The oral treatment of the animals with WEDC (1-10 mg/kg) produced a significant, dose-dependent reduction of articular incapacitation and abdominal contortions in the writhing test. The same effect was not observed in the hot plate and rota-rod tests. WEDC also reduced @entity3542 release into the zymosan-inflamed joints. In the evaluation of COX activity, we observed that WEDC was able to selectively inhibit @entity4645 but not @entity9774 activity in COS-7 cells. Moreover, WEDC treatment did not show @entity344 . Our data confirm the anti-nociceptive activities of the WEDC and indicate that this effect could be associated with inhibition of @entity4645 ( @entity4645 ) and @entity842 release. The effects could be attributed to cucurbitacins since several of these were isolated from the WEDC.

XXXX and cyclooxygenase may participate in the analgesic and anti-inflammatory effect of the cucurbitacins fraction from Wilbrandia ebracteata.

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity627 :: ('MESH:D012216', 'Disease') :: ['rheumatic diseases'] @entity625 :: ('MESH:D001168', 'Disease') :: ['arthritis'] @entity1044 :: ('MESH:D008752', 'Chemical') :: ['dichloromethane'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity3542 :: ('CHEBI:16301', 'Chemical') :: ['nitrite'] @entity344 :: ('MESH:D005767', 'Disease') :: ['gastrointestinal toxicity'] @entity9774 :: ('26195(Tax:10116)', 'Gene') :: ['COX-1'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity158 :: ('MESH:D010146', 'Disease') :: ['pain'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity4645 :: ('29527(Tax:10116)', 'Gene') :: ['COX-2', 'cyclooxygenase-2']
@entity842 :: (MESH:D009569,Chemical) :: ['Nitric oxide']
