UNASSIGNED: Sporadic @entity14 with @entity124 ( @entity124 ) frequently contain a mutation of the @entity817 gene. Additionally, it has been shown that @entity817 mutations in @entity14 are mutually exclusive of @entity984 mutation. We evaluated 14 cases of @entity14 with @entity124 that were @entity817 wild type but demonstrated a @entity984 mutation. The codon 12/13 region in exon 2 of the @entity984 oncogene and the codon 600 region in exon 15 of the @entity817 gene were analyzed with standard PCR methods. @entity124 was evaluated by using the Bethesda panel of markers. The methylation status of the mismatch repair system was ascertained using the SALSA   MS-MLPA   methylation-specific DNA detection. The mismatch repair proteins @entity6756 , @entity4311 , @entity4638 , and @entity14437 were evaluated by immunohistochemical staining. A total of 530 @entity14 were studied for @entity124 and @entity984 gene mutation. @entity5 with both @entity124 and a @entity984 mutation were identified, and all @entity5 were @entity817 wild type. Methylation was present in 7 (50%), 5 demonstrated methylation of @entity6756 , 1 showed methylation of @entity8724 , and 1 showed methylation of @entity4311 . Four @entity1 had simultaneous @entity5 , some of which showed different genetic changes. Immunohistochemical staining suggested a germ line mutation for 4 of 10 cases with complete staining information. @entity984 mutation may occur with @entity124 in @entity14 with wild-type @entity817 . If a mutation in @entity984 co-exists with @entity124 , then strong methylation of the @entity6756 gene is unlikely. These @entity5 demonstrate that a small number of @entity14 will develop with atypical patterns of molecular genetic changes, suggesting that a specific pattern of genetic changes may not be as crucial as the overall accumulation of changes, consistent with the 'unique @entity5 principle'.

XXXX with the Uncommon Findings of @entity984 Mutation and Microsatellite Instability.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity6756 :: ('4292', 'Gene') :: ['MLH1'] @entity124 :: ('MESH:D053842', 'Disease') :: ['MSI', 'microsatellite instability'] @entity984 :: ('3845', 'Gene') :: ['KRAS'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors', 'cancers', 'Fourteen (2.6%) cancers'] @entity4311 :: ('4436', 'Gene') :: ['MSH2'] @entity14437 :: ('5395', 'Gene') :: ['PMS2'] @entity8724 :: ('4255', 'Gene') :: ['MGMT'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal cancers', 'colorectal cancer'] @entity817 :: ('673', 'Gene') :: ['BRAF'] @entity4638 :: ('2956', 'Gene') :: ['MSH6']
@entity14 :: (MESH:D015179,Disease) :: ['Colorectal Cancers']
