On the basis of sequence similarities to the @entity375 @entity62101 and @entity10133 gene, the @entity35 alternatively spliced mRNA has been suggested to be a Golgi secretory pathway @entity141 -ATPase ( @entity10133 ). Data in this report lend further support for this hypothesis in that @entity969 gradient fractionation of @entity35 liver microsomes resulted in @entity10133 comigrating with the Golgi @entity460 binding protein @entity70488 , which was well resolved from the endoplasmic reticulum marker @entity66104 . Also, in PC-12 cells, antibody to @entity10133 colocalized with an antibody to the Golgi marker @entity54727 . To study the biological effects of @entity10133 expression, we performed stable overexpression of @entity10133 in COS-7 cells. Seven clones were selected for further comparison with COS-7 cells containing an empty expression vector. Overexpression of @entity10133 resulted in a significant reduction of plasma membrane @entity141 -ATPase, sarco(endo)plasmic reticulum @entity141 -ATPase, and @entity66104 expression in these clones. In contrast, the expression of the Golgi @entity460 -binding protein @entity70488 increased significantly. The phosphoenzyme intermediate formed using membranes from clone G11/5 was @entity460 dependent, significantly more intense than in COS-7 cells, and not affected by @entity11329 treatment. @entity460 uptake by G11/5 microsomes was @entity855 dependent and significantly greater than in microsomes from parent COS-7 cells. The overexpression of @entity10133 significantly increased the growth rate of these cells compared with COS-7 cells containing only the empty vector. These data demonstrate that overexpression of the @entity35 @entity10133 results in significant changes in the expression of @entity460 transport and storage proteins in COS-7 cells.

Characterization of Cos-7 cells overexpressing the @entity35 secretory pathway XXXX -ATPase.

@entity11329 :: ('CHEBI:49701', 'Chemical') :: ['La3+'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+'] @entity66104 :: ('64202(Tax:10116)', 'Gene') :: ['calreticulin'] @entity54727 :: ('25478(Tax:10116)', 'Gene') :: ['alpha-mannosidase II'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity10133 :: ('2155', 'Gene') :: ['SPCA', 'hSPCA'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium', 'Calcium'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity70488 :: ('84595(Tax:10116)', 'Gene') :: ['CALNUC'] @entity375 :: ('4932', 'Species') :: ['yeast'] @entity62101 :: ('852709(Tax:4932)', 'Gene') :: ['PMR1']
@entity141 :: (CHEBI:29108,Chemical) :: ['Ca2+']
