OBJECTIVE: This study examined the effects of a growth factor, recombinant @entity1 @entity12017 (rhOP-1), on the formation of tissue-engineered cartilaginous tissue by adult @entity291 articular chondrocytes using the alginate-recovered-chondrocyte (ARC) method. DESIGN: To ascertain if rhOP-1 enhances the formation of the @entity1358 ( @entity1358 ) and the characteristics of @entity1358 formation, @entity291 articular chondrocytes were first cultured for up to 14 days in alginate beads in medium supplemented with serum, with or without rhOP-1. Then, the recovered chondrocytes and their associated @entity1358 were resuspended in medium, with or without OP-1, seeded onto culture inserts, and incubated for an additional 14 days. The fabricated ARC tissues were subjected to biochemical and histological analyses. RESULTS: The addition of rhOP-1 to the medium in the alginate bead culture step resulted in an increased accumulation of both proteoglycan (PG) and collagen, with a ratio of PG to collagen that was higher than that found in native adult cartilage. The addition of rhOP-1 in the second step had a similar stimulatory effect during 14 days of culture. Histological examination of the tissue formed under all conditions revealed a cartilage-like matrix, stained strongly by @entity11979 . The thickness of the tissues obtained from culture conditions that included the addition of rhOP-1 was four times greater than that of the tissues cultured without rhOP-1. CONCLUSIONS: Using the ARC method, rhOP-1 enhanced the formation of matrix and generated a voluminous tissue-engineered cartilaginous construct. These characteristics may be beneficial in generating constructs that can cover large defects.

XXXX promotes the formation of tissue-engineered cartilage using the alginate-recovered-chondrocyte method.

@entity1 :: ('9606', 'Species') :: ['human'] @entity11979 :: ('MESH:D014048', 'Chemical') :: ['toluidine blue'] @entity12017 :: ('655', 'Gene') :: ['osteogenic protein-1'] @entity1358 :: ('MESH:D002292', 'Disease') :: ['cell-associated matrix', 'CM'] @entity291 :: ('9913', 'Species') :: ['bovine']
@entity12017 :: (655,Gene) :: ['Osteogenic protein-1']
