OBJECTIVE: To evaluate the therapeutic potential of @entity4020 ( @entity4020 ) in @entity571 ( @entity571 ) and @entity309 ( @entity309 ) by examining regulation of cytokine antagonist expression. METHODS: Expression of @entity1193 ( @entity1193 ) and soluble @entity5 necrosis factor receptor (sTNFR) was examined by ELISA in cells from freshly isolated synovial fluids (SF) and synovial tissues (ST) from @entity1 with @entity571 or @entity309 , either left untreated or treated with @entity4020 . Single (7) and paired (5) SF and ST cells from @entity571 and @entity309 @entity1 were examined. As well, the ability of @entity4020 to regulate gene expression levels for @entity2159 ( @entity2159 ) and @entity5298 ( @entity5298 ) was examined in freshly isolated SF cells from @entity1 with @entity309 , by reverse transcriptase polymerase chain reaction. RESULTS: @entity1193 and sTNFR were found to be constitutively expressed in @entity571 and @entity309 SF and ST cells. @entity4020 treatment resulted in an increase in both @entity1193 and sTNFR production. Freshly isolated @entity309 SF cells exhibited constitutive @entity5298 gene expression in both the @entity784 and T cell fractions of the SF. In contrast, @entity2159 gene expression levels were undetectable or low. @entity4020 treatment of @entity309 SF cells resulted in upregulation of @entity2159 gene expression in the T cell fraction of the @entity309 SF cells, whereas @entity5298 gene expression remained unaffected. CONCLUSION: These in vitro data suggest a therapeutic role for @entity4020 in the treatment of @entity625 through upregulation of critical cytokine antagonists.

Therapeutic implications for interferon-alpha in XXXX : a pilot study.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity571 :: ('MESH:D010003', 'Disease') :: ['osteoarthritis', 'OA'] @entity625 :: ('MESH:D001168', 'Disease') :: ['arthritis'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity784 :: ('MESH:D016399', 'Disease') :: ['non-T'] @entity1193 :: ('3557', 'Gene') :: ['IL 1Ra', 'interleukin 1 receptor antagonist', 'IL-1Ra'] @entity5298 :: ('8600', 'Gene') :: ['osteoprotegerin ligand', 'OPGL'] @entity4020 :: ('3440', 'Gene') :: ['IFN-a', 'interferon-a'] @entity309 :: ('MESH:D001172', 'Disease') :: ['rheumatoid arthritis', 'RA'] @entity2159 :: ('4982', 'Gene') :: ['osteoprotegerin', 'OPG']
@entity625 :: (MESH:D001168,Disease) :: ['arthritis']
