1. The effects of insulin in vitro on tissue pools and incorporation into protein of @entity2228 and @entity368 in the extensor digitorum longus muscle of the @entity35 are reported. 2. It was found that insulin decreased the lag period before the establishment of a linear rate of incorporation of radioactive @entity2228 into protein. 3. The hormone increased the size of the free intracellular @entity2228 pool. No such effect was found for @entity368 . The accumulation of radioactive @entity2228 in the intracellular fluid compartment was increased. The content of radioactive @entity368 in the intracellular compartment was decreased. 4. Insulin decreased the specific radioactivity of both @entity2228 and @entity368 in the extracellular fluid. 5. The hormone also decreased protein catabolism. 6. The effect on protein synthesis was not caused by an increase in the specific radioactivity of the extracellular pool but was possibly related to increased @entity535 concentrations in this pool, which could in turn have affected the aggregation of ribosomes.

The effect of insulin on free @entity535 pools and protein synthesis in XXXX skeletal muscle in vitro.

@entity35 :: ('10116', 'Species') :: ['rat'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity2228 :: ('CHEBI:57305', 'Chemical') :: ['glycine'] @entity368 :: ('CHEBI:25017', 'Chemical') :: ['leucine']
@entity35 :: (10116,Species) :: ['rat']
