UNASSIGNED: During bone formation in embryos, large amounts of @entity460 and @entity4613 are taken up and transported to the site where solid mineral is first deposited. The initial mineral forms in vesicles inside osteoblasts and is deposited as a highly disordered @entity78 phase. The mineral is then translocated to the extracellular space where it penetrates the collagen matrix and crystallizes. To date little is known about the transport mechanisms of @entity460 and @entity4613 in the vascular system, especially when high transport rates are needed and the concentrations of these ions in the blood serum may exceed the solubility product of the mineral phase. Here we used a rapidly growing biological model, the @entity1573 embryo, to study the bone mineralization pathway taking advantage of the fact that large amounts of bone mineral constituents are transported. Cryo scanning electron microscopy together with cryo energy dispersive X-ray spectroscopy and focused-ion beam imaging in the serial surface view mode surprisingly reveal the presence of abundant vesicles containing small mineral particles in the lumen of the blood vessels. Morphologically similar vesicles are also found in the cells associated with bone formation. This observation directly implicates the vascular system in solid mineral distribution, as opposed to the transport of ions in solution. Mineral particle transport inside vesicles implies that far larger amounts of the bone mineral constituents can be transported through the vasculature, without the danger of ectopic precipitation. This introduces a new stage into the bone mineral formation pathway, with the first mineral being formed far from the bone itself.

Transport of membrane-bound mineral particles in blood vessels during XXXX embryonic bone development.

@entity1573 :: ('9031', 'Species') :: ['chick'] @entity78 :: ('MESH:C020243', 'Chemical') :: ['calcium phosphate'] @entity4613 :: ('D010710', 'Chemical') :: ['phosphate'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium']
@entity1573 :: (9031,Species) :: ['chicken']
