Intracellular membrane traffic is thought to be regulated in part by SNAREs, integral membrane proteins on transport vesicles (v-SNAREs) and target organelles (t-SNAREs) that bind to each other and mediate bilayer fusion. All known SNARE-mediated fusion events involve a member of the @entity44342 family of t-SNAREs. Sequence comparisons identify eight such proteins encoded in the @entity375 genome, of which six have been characterized. We describe here the remaining two, @entity70176 and @entity70177 . These have the expected biochemical properties of t-SNAREs, and are located in separable compartments which correspond to a putative early endosome and the @entity375 equivalent of the TGN, respectively. They co-precipitate with the v-SNARE @entity25565 , which is implicated in Golgi-endosome traffic and, remarkably, binds to five different syntaxins. @entity70176 also binds the plasma membrane v-SNARE @entity18738 . Both @entity70176 and @entity70177 are required for efficient endocytosis and to maintain normal levels of TGN proteins. However, neither is required for intra-Golgi traffic. Since no further syntaxins have been identified in @entity375 , this implies that the Golgi apparatus can function with a single @entity44342 , @entity44137 .

Two @entity44342 homologues in the TGN/endosomal system of XXXX .

@entity70176 :: ('852079(Tax:4932)', 'Gene') :: ['Tlg1p'] @entity44342 :: ('851219(Tax:4932)', 'Gene') :: ['syntaxin'] @entity70177 :: ('854142(Tax:4932)', 'Gene') :: ['Tlg2p'] @entity25565 :: ('855237(Tax:4932)', 'Gene') :: ['Vti1p'] @entity44137 :: ('850713(Tax:4932)', 'Gene') :: ['Sed5p'] @entity375 :: ('4932', 'Species') :: ['yeast'] @entity18738 :: ('851203(Tax:4932)', 'Gene') :: ['Snc1p']
@entity375 :: (4932,Species) :: ['yeast']
