Dried blood spot (DBS) methodology offers significant advantages over venipuncture in studies of vulnerable populations or large-scale studies, including reduced @entity1 burden and higher response rates. Uncertainty about the validity of @entity65 ( @entity65 ) risk biomarkers remains a barrier to wide-scale use. We determined the validity of DBS-derived biomarkers of @entity65 risk versus gold-standard assessments, and study-specific, serum-equivalency values for clinical relevance of DBS-derived values. Concurrent venipuncture serum and DBS samples (n = 150 adults) were assayed in Clinical Laboratory Improvement Amendments-certified and DBS laboratories, respectively. Time controls of DBS standard samples were assayed single-blind along with test samples. Linear regression analyses evaluated DBS-to-serum equivalency values; agreement and bias were assessed via Bland-Altman plots. Linear regressions of venipuncture values on DBS-to-serum equivalencies provided R(2) values for total @entity165 , high-density lipoprotein @entity165 (HDL-C), and @entity1851 ( @entity1851 ) of 0.484, 0.118, and 0.666, respectively. Bland-Altman plots revealed minimal systematic bias between DBS-to-serum and venipuncture values; precision worsened at higher mean values of @entity1851 . Time controls revealed @entity306 or change in analyte values for HDL-C and @entity1851 over 30 weeks. We concluded that DBS-assessed biomarkers represent a valid alternative to venipuncture assessments. Large studies using DBS should include study-specific serum-equivalency determinations to optimize individual-level sensitivity, the viability of detecting intervention effects, and generalizability in community-level primary prevention interventions.

Validation of Biomarkers of XXXX Risk from Dried Blood Spots in Community-Based Research: Methodologies and Study-Specific Serum Equivalencies.

@entity1 :: ('9606', 'Species') :: ['participant'] @entity1851 :: ('1401', 'Gene') :: ['C-reactive protein', 'CRP'] @entity65 :: ('MESH:D002318', 'Disease') :: ['CVD', 'cardiovascular disease'] @entity165 :: ('MESH:D002784', 'Chemical') :: ['cholesterol'] @entity306 :: ('MESH:D002547', 'Disease') :: ['little degradation']
@entity65 :: (MESH:D002318,Disease) :: ['CVD']
