When ectotherms are exposed to low temperatures, they enter a cold-induced @entity772 (chill @entity772 ) that prevents resource acquisition, mating, oviposition, and escape from predation. There is substantial variation in time taken to recover from chill @entity772 both within and among species, and this variation is correlated with habitat temperatures such that insects from cold environments recover more quickly. This suggests an adaptive response, but the mechanisms underlying variation in recovery times are unknown, making it difficult to decisively test adaptive hypotheses. We use replicated lines of @entity238 selected in the laboratory for fast (hardy) or slow (susceptible) chill- @entity772 recovery times to investigate modifications to metabolic profiles associated with cold adaptation. We measured metabolite concentrations of flies before, during, and after cold exposure using NMR spectroscopy to test the hypotheses that hardy flies maintain metabolic homeostasis better during cold exposure and recovery, and that their metabolic networks are more robust to cold-induced perturbations. The metabolites of cold-hardy flies were less cold responsive and their metabolic networks during cold exposure were more robust, supporting our hypotheses. Metabolites involved in membrane lipid synthesis, @entity2340 metabolism, oxidative stress, energy balance, and @entity1521 metabolism were altered by selection on cold tolerance. We discuss the potential significance of these alterations. This article is protected by copyright. All rights reserved.

Cold adaptation shapes the robustness of metabolic networks in XXXX .

@entity1521 :: ('CHEBI:26271', 'Chemical') :: ['proline'] @entity2340 :: ('CHEBI:27897', 'Chemical') :: ['tryptophan'] @entity238 :: ('7227', 'Species') :: ['Drosophila melanogaster'] @entity772 :: ('MESH:D003128', 'Disease') :: ['coma']
@entity238 :: (7227,Species) :: ['Drosophila melanogaster']
