UNASSIGNED: Using the transcription activator-like effectors nucleases-mediated gene knockout technology, we have previously demonstrated that LH signaling is required for oocyte maturation and ovulation but is dispensable for testis development in @entity3392 . Here, we have further established the fshb and @entity68427 knockout @entity3392 lines. In females, fshb mutant is subfertile, whereas @entity68427 mutant is @entity249 . Folliculogenesis is partially affected in the fshb mutant but is completely arrested at the primary growth stage in the @entity68427 mutant. In males, fshb and @entity68427 mutant are fertile. The fertilization rate and histological structure of the testis is not affected. However, double knockout of fshb; @entity68428 or @entity68427 ;lhr leads to all @entity249 male offspring. The key @entity479 hormones and steroidogenic genes are dramatically decreased in double knockout mutant (fshb; @entity68428 and @entity68427 ;lhr) but not in single knockout mutant (fshb, @entity68428 , @entity68427 , and lhr) males. Furthermore, we have also demonstrated the constitutive activities of both @entity68427 ( @entity68427 ) and LH receptor in @entity3392 and the compensatory role of LH by cross-reacting with @entity68427 in the fshb;lhr double mutant, thus explaining the phenotypic discrepancy observed among the ligand/receptor mutant lines. Taken together, our data established the following models on the roles of gonadotropin signaling in @entity3392 gonad development. In females, FSH signaling is mainly responsible for promoting follicular growth, whereas LH signaling is mainly responsible for stimulating oocyte maturation and ovulation. In males, the functions of FSH and LH signaling overlap, and only disruption of both FSH and LH signaling could lead to the @entity249 phenotype. In the absence of FSH, LH could play a compensatory role by cross-reacting with @entity68427 in both male and female.

Gonadotropin Signaling in XXXX Ovary and Testis Development: Insights From Gene Knockout Study.

@entity249 :: ('MESH:D007246', 'Disease') :: ['infertile'] @entity68428 :: ('402917(Tax:7955)', 'Gene') :: ['lhb'] @entity68427 :: ('195820(Tax:7955)', 'Gene') :: ['FSH receptor', 'fshr', 'FSHR'] @entity3392 :: ('7955', 'Species') :: ['zebrafish'] @entity479 :: ('D013256', 'Chemical') :: ['steroid']
@entity3392 :: (7955,Species) :: ['Zebrafish']
