UNASSIGNED: A central step in the initiation of chromosomal DNA replication in eukaryotes is the assembly of pre-replicative complex (pre-RC) at late M and early G1 phase of the cell cycles. Since 1973, four proteins or protein complexes, including cell division control protein 6 (Cdc6)/Cdc18, minichromosome @entity22 ( @entity22 ), origin recognition complex (ORC), and Cdt1, are known components of the pre-RC. Previously, we reported that a non-ORC protein binds to the essential element delta 9 of the @entity6739 DNA replication origin ARS3001. In this study, we identified that the non-ORC protein is Sap1. Like ORC, Sap1 binds to DNA origins during cell growth cycles. But unlike ORC, which binds to asymmetric AT-rich sequences through its nine AT-hook motifs, Sap1 preferentially binds to a DNA sequence of 5-( @entity2775 )nC/G @entity69196 ( @entity2775 )n-3. We also found that Sap1 and ORC physically interact. We further demonstrated that Sap1 is required for the assembly of the pre-RC because of its essential role in recruiting Cdc18 to DNA origins. Thus, we conclude that Sap1 is a replication-initiation factor that directly participates in the assembly of the pre-RC. DNA replication origins in @entity6739 are defined by possessing two essential elements with one bound by ORC and the other by Sap1.

Sap1 is a replication initiation factor essential for the assembly of pre-replicative complex in the XXXX @entity6739 .

@entity6739 :: ('4896', 'Species') :: ['Schizosaccharomyces pombe', 'fission yeast'] @entity69196 :: ('|SUB|A,G|)9,10|C', 'ProteinMutation') :: ['(A/T)9-10G/C'] @entity2775 :: ('c|SUB|A||T', 'DNAMutation') :: ['A/T'] @entity22 :: ('MESH:D007319', 'Disease') :: ['MCM', 'maintenance protein complex']
@entity6739 :: (4896,Species) :: ['fission yeast']
