UNASSIGNED: Chromatin undergoes structural changes in response to extracellular and environmental signals. We observed changes in nuclear morphology in @entity5 tissue biopsied after chemotherapy and hypothesised that these DNA damage-induced changes are mediated by histone deacetylases (HDACs). Nuclear morphological changes in cell lines (PE01 and PE04 models) and a xenograft model (OV1002) were measured in response to @entity902 chemotherapy by image analysis of nuclear texture. @entity15687 expression increased in PEO1 cells treated with @entity900 at 24h, which was accompanied by increased expression of @entity12061 ( @entity12061 ). @entity15687 and @entity12061 expression were also increased after @entity3003 treatment in the OV1002 @entity3003 -sensitive xenograft model but not in the insensitive HOX424 model. Expression of DNA damage response pathways (pBRCA1, yH2AX, pATM, pATR) showed time-dependent changes after @entity900 treatment. @entity15687 knockdown by siRNA reduced @entity12061 expression, induced DNA double strand breaks (DSB) measured by yH2AX, and interfered with the activation of DNA damage response induced by @entity900 . Furthermore, @entity15687 depletion affected yH2AX foci formation, cell cycle distribution, and apoptosis triggered by @entity900 , and was additive to the inhibitory effect of @entity900 in cell lines. By inhibiting expression of @entity15687 , reversible alterations in chromatin patterns during @entity900 treatment were observed. These results demonstrate quantifiable alterations in nuclear morphology after chemotherapy, and implicate @entity15687 in higher order chromatin changes and cellular DNA damage responses in @entity43 cells in vitro and in vivo.

The role of @entity15687 in chromatin remodelling and response to chemotherapy in XXXX .

@entity43 :: ('MESH:D010051', 'Disease') :: ['ovarian cancer'] @entity12061 :: ('23468', 'Gene') :: ['HP1', 'heterochromatin protein 1'] @entity15687 :: ('3066', 'Gene') :: ['HDAC2'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity3003 :: ('MESH:D016190', 'Chemical') :: ['carboplatin'] @entity900 :: ('MESH:D002945', 'Chemical') :: ['cisplatin'] @entity902 :: ('MESH:D010984', 'Chemical') :: ['platinum']
@entity43 :: (MESH:D010051,Disease) :: ['ovarian cancer']
