BACKGROUND: @entity1 embryonic stem cells (hESCs) partially recapitulate early embryonic three germ layer development, allowing testing of potential teratogenic hazards. Because use of hESCs is ethically debated, we investigated the potential for @entity1 induced pluripotent stem cells (hiPSCs) to replace hESCs in such tests. METHODS: Three cell lines, comprising hiPSCs (foreskin and IMR90) and hESCs (H9) were differentiated for 14  days. Their transcriptome profiles were obtained on day 0 and day 14 and analyzed by comprehensive bioinformatics tools. RESULTS: The transcriptomes on day 14 showed that more than 70% of the "developmental genes" (regulated genes with   >   2-fold change on day 14 compared to day 0) exhibited variability among cell lines. The developmental genes belonging to all three cell lines captured biological processes and KEGG pathways related to all three germ layer embryonic development. In addition, transcriptome profiles were obtained after 14  days of exposure to teratogenic @entity1092 ( @entity3262 ) during differentiation. Although the differentially regulated genes between treated and untreated samples showed more than 90% variability among cell lines, @entity3262 clearly antagonized the expression of developmental genes in all cell lines: suppressing upregulated developmental genes, while inducing downregulated ones. To quantify @entity3262 -disturbed development based on developmental genes, we estimated the "developmental potency" (D p ) and "developmental index" (D i ). CONCLUSIONS: Despite differences in genes deregulated by @entity3262 , uniform D i values were obtained for all three cell lines. Given that the D i values for @entity3262 were similar for hESCs and hiPSCs, D i can be used for robust hazard identification, irrespective of whether hESCs or hiPSCs are used in the test systems.

Comparison of a teratogenic transcriptome-based predictive test based on XXXX embryonic versus inducible pluripotent stem cells.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity1092 :: ('MESH:D014635', 'Chemical') :: ['valproic acid'] @entity3262 :: ('D014635', 'Chemical') :: ['VPA']
@entity1 :: (9606,Species) :: ['human']
