UNASSIGNED: A novel @entity1 ferritin-based nanocarrier, composed of 24 modified monomers able to auto-assemble into a modified protein cage, was produced and used as selective carrier of anti- @entity5 payloads. Each modified monomer derives from the genetic fusion of two distinct modules, namely the heavy chain of @entity1 ferritin (HFt) and a stabilizing/protective PAS polypeptide sequence rich in @entity1521 (P), @entity563 (S), and @entity3929 (A) residues. Two genetically fused protein constructs containing PAS polymers with 40- and 75-residue lengths, respectively, were compared. They were produced and purified as recombinant proteins in @entity526 at high yields. Both preparations were highly soluble and stable in vitro as well as in @entity19 plasma. Size-exclusion chromatography, dynamic light scattering, and transmission electron microscopy results indicated that PASylated ferritins are fully assembled and highly monodispersed. In addition, yields and stability of encapsulated @entity632 were significantly better for both HFt-PAS proteins than for wild-type HFt. Importantly, PAS sequences considerably prolonged the half-life of HFt in the @entity19 bloodstream. Finally, our @entity632 -loaded nanocages preserved the pharmacological activity of the drug. Taken together, these results indicate that both of the developed HFt-PAS fusion proteins are promising nanocarriers for future applications in @entity5 therapy.

Improved @entity632 Encapsulation and Pharmacokinetics of Ferritin-Fusion Protein Nanocarriers Bearing @entity1521 , @entity563 , and XXXX Elements.

@entity1 :: ('9606', 'Species') :: ['human'] @entity632 :: ('MESH:D004317', 'Chemical') :: ['doxorubicin'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity3929 :: ('D000409', 'Chemical') :: ['alanine'] @entity563 :: ('CHEBI:17822', 'Chemical') :: ['serine'] @entity1521 :: ('CHEBI:26271', 'Chemical') :: ['proline'] @entity526 :: ('562', 'Species') :: ['Escherichia coli']
@entity3929 :: (D000409,Chemical) :: ['Alanine']
