The aim of the present investigation was to isolate a probiotic strain from 23 samples of yurts cheese and 21 samples of kumiss (collected from scattered households in Xinjiang and Inner Mongolia), and from eN-Lac Capsules, a health-promoting product. The isolates were subjected to biochemical characterization analysis and were tested for tolerance to low pH, @entity1372 salt, @entity6741 , pepsin, and trypsin. 16S DNA sequence analysis was conducted to identify the strain. The possible dose-dependent role of strain LP2 in immunomodulation was investigated using the ICR @entity19 model (from the Institute of @entity5 ). Daily, we conducted clinical observations, a @entity505 clearance test, a spleen lymphocyte proliferation test, and measurements of body mass and lymphoid organ index. Natural killer cell activity and @entity56 reaction were determined. The results showed that 3 selected strains (LP2, LP4, and LP9) had high tolerance to low pH, @entity2827 , and @entity6741 and were not significantly different from @entity6983 in terms of morphology, colony, and biochemistry characterizations. A further tolerance test showed that LP2 had the highest survival rate (90%) under the conditions of pH 3.0, 0.3% @entity6741 , 10 mg/mL pepsin, and 10 mg/mL trypsin for 24 h. The sequence heterogeneities within the 16S rDNA genes molecularly elucidated that the LP2 belongs to the @entity6983 family, on the basis of a homology of 99.6%. A significant enhanced footpad swelling reaction and natural killer cell activity in the middle-dose (10(8) cfu/mL) and the high-dose (10(9) cfu/mL) groups were observed but without obvious dose dependence (P < 0.05). Lymphocyte proliferation was also increased significantly in a dose-dependent manner (P < 0.01) compared with that of the control group, indicating a positive immunoregulatory effect.

A tolerant lactic acid bacteria, XXXX , and its immunoregulatory function.

@entity19 :: ('10090', 'Species') :: ['mouse'] @entity5 :: ('MESH:D009369', 'Disease') :: ['Cancer Research'] @entity1372 :: ('MESH:D012964', 'Chemical') :: ['sodium'] @entity6741 :: ('CHEBI:3098', 'Chemical') :: ['bile salt'] @entity56 :: ('MESH:D004342', 'Disease') :: ['delayed-type hypersensitivity'] @entity6983 :: ('1597', 'Species') :: ['Lactobacillus paracasei', 'L. paracasei'] @entity505 :: ('MESH:D002244', 'Chemical') :: ['carbon'] @entity2827 :: ('MESH:D012965', 'Chemical') :: ['sodium chloride']
@entity6983 :: (1597,Species) :: ['Lactobacillus paracasei']
