PURPOSE: @entity43 is a highly lethal gynecological malignancy for which the overall prognosis has remained poor over the past few decades. Interleukin ( @entity650 ) has been found to be a major contributor to the initiation and progression of @entity43 . This cytokine exerts its activity through activation of several signaling pathways, in particular the signal transducer and activator of transcription ( @entity4104 ) pathway. Here, we aimed at investigating the capacity of a natural dietary compound found in cruciferous vegetables, i.e., @entity14507 ( @entity12315 ), to target the metastatic phenotype of @entity43 cells through functional p- @entity4104 . METHODS: The @entity1 @entity43 -derived cell lines SKOV3 and A2780 were treated with @entity650 and/or @entity12315 and subjected to in vitro proliferation, adhesion, migration and invasion assays to assess the anti-metastatic and anti- @entity650 effects of @entity12315 , as well as to assess gene expression alterations before and after shRNA-mediated @entity4104 silencing. RESULTS: We found that @entity12315 inhibits @entity650 -mediated increases in @entity43 cell adhesion, migration and invasion. These results were corroborated by shRNA-mediated @entity4104 silencing. Through Western blot and ELISA analyses direct evidence was provided for the capacity of @entity12315 to inhibit @entity43 cell adhesion, migration and invasion, which was found to be associated with down-regulation of the matrix metalloproteinases @entity1476 and @entity1495 . CONCLUSIONS: From our data we conclude that @entity12315 exhibits an anti- @entity650 -like activity by inhibiting p- @entity4104 enhanced @entity43 cell proliferation and in vitro @entity3 -associated events, i.e., adhesion, migration and invasion. Most significantly, @entity1476 and @entity1495 , which are known to promote and enhance @entity3 , were found to act as targets of @entity12315 . This anti- @entity650 -like property of @entity12315 may pave the way for the development of novel @entity43 preventive and/or therapeutic strategies.

@entity650 -induced @entity3 modulators p- XXXX , @entity1476 and @entity1495 are targets of @entity14507 in @entity43 cells.

@entity43 :: ('MESH:D010051', 'Disease') :: ['ovarian carcinoma', 'ovarian cancer', 'Ovarian cancer'] @entity650 :: ('3569', 'Gene') :: ['IL6'] @entity14507 :: ('MESH:C016392', 'Chemical') :: ["3,3'-diindolylmethane"] @entity1 :: ('9606', 'Species') :: ['human'] @entity1476 :: ('4313', 'Gene') :: ['MMP-2'] @entity4104 :: ('6774', 'Gene') :: ['STAT3'] @entity12315 :: ('CHEBI:50182', 'Chemical') :: ['DIM'] @entity1495 :: ('4318', 'Gene') :: ['MMP-9'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis']
@entity4104 :: (6774,Gene) :: ['STAT3']
