Objective To investigate the changes of @entity29975 /b-catenin signaling pathway molecules in the differentiation of fetal @entity35 alveolar epithelial type II cells (fAEC2). Methods Fetal @entity35 lung tissues were extracted from pregnant SD @entity35 (19 days), and then fAEC2 were isolated and purified. After fAEC2 were cultured for 48, 72, 96 hours, the morphological changes of fAEC2 and the expression of b-catenin were respectively observed by inverted microscope and immunofluorescence. The mRNA expressions of @entity29975 , @entity12003 , pulmonary surfactant C ( @entity10459 ) and @entity24941 ( @entity24941 ) were detected by real-time quantitative PCR. The protein expressions of @entity12003 , nucleus b-catenin, @entity10459 and @entity24941 were measured by Western blotting. Results b-catenin was expressed in cell membrane when fAEC2 were cultured for 48 hours; the expression of b-catenin decreased in cell membrane while enhanced in cytoplasm and nucleus at 72 hours; whole-cell b-catenin expression was lowered at 96 hours. The expressions of @entity29975 and @entity12003 mRNAs were significantly raised at 72 hours and reduced obviously at 96 hours compared with 48 hours. @entity10459 mRNA expression level went down gradually with the extended culture time, and @entity24941 mRNA level went up gradually. The protein expressions of @entity12003 and nucleus b-catenin were significantly enhanced at 72 hours and weakened at 96 hours compared with 48 hours. @entity10459 protein expression was down-regulated with the prolonged culture time, and @entity24941 protein expression was up-regulated. Conclusion @entity29975 /b-catenin signaling pathway may play an important role in fAEC2 transdifferentiation.

[The changes of XXXX /b-catenin signaling pathway molecules in the differentiation of fetal alveolar epithelial type II cells].

@entity24941 :: ('25241(Tax:10116)', 'Gene') :: ['AQP5', 'aquaporin 5'] @entity29975 :: ('315196(Tax:10116)', 'Gene') :: ['Wnt7b'] @entity12003 :: ('58919(Tax:10116)', 'Gene') :: ['cyclin D1'] @entity10459 :: ('50683(Tax:10116)', 'Gene') :: ['SP-C'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats']
@entity29975 :: (315196(Tax:10116),Gene) :: ['Wnt7b']
