To evaluate the role of excitatory @entity1522 in secondary injury occurring after @entity1566 , several experimental studies focusing on the the changes of @entity535 levels in the spinal cord have been performed to date. However, because of technical limitations, it has not been possible to correlate the local changes of excitatory @entity1522 with the total tissue levels of excitatory @entity1522 . To investigate the connection between the spread of injury and the excitatory @entity1522 , we assessed, the local changes of @entity492 through novel experimental approaches like immunoreactivity via fluorescence microphotometry and histopathology while also analyzing the total tissue levels of @entity1522 via HPLC. These studies were performed using a model of incomplete cervical @entity1566 in @entity35 . Through this approach, we found that the levels of excitatory @entity1522 , such as @entity2227 and @entity492 , began to decrease immediately after injury. No significant decrease was observed in the other @entity1522 . Similarly, local changes in @entity492 in the spinal cord were observed using fluorescence microphotometry. The decrease in the anterior and posterior horns was rapid up to 15 min after injury, but, slowed thereafter, suggesting that a release of excitatory @entity1522 occurred at the site of primary injury almost immediately following injury. At 15-min post-injury large neurons within the injured cord appeared intact on histopathological analysis demonstrating that the alteration of excitatory @entity1522 occurs prior to histopathological change. Histopathological change in the white matter occurred more slowly than in the anterior and posterior horns, suggesting the spread of the lesion by secondary damage due to an autoclastic mechanism.

Changes of @entity535 levels and XXXX distribution in the cervical spinal cord after traumatic @entity1566 .

@entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity2227 :: ('CHEBI:29987', 'Chemical') :: ['glutamate'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity492 :: ('CHEBI:29995', 'Chemical') :: ['aspartate'] @entity1566 :: ('MESH:D013119', 'Disease') :: ['spinal cord injury', 'spinal cord trauma']
@entity492 :: (CHEBI:29995,Chemical) :: ['aspartate']
