@entity1082 ) is found in most @entity1 with high-grade @entity3146 ( @entity3146 ) 2/3 in cervical cytology and biopsies. Multiple high-risk HPV (hrHPV) genotypes are present in 15% to 50% of cytology samples. We have shown by laser-capture microscopy (LCM)-polymerase chain reaction (PCR) that each lesion is associated with a single hrHPV type. Attribution of hrHPV types to CIN2/3 is important to understand the oncogenic role of different types and the limitations of cytologic typing. We studied hrHPV genotypes in 257 @entity1 with histologic CIN2/3 referred on the basis of abnormal cytology. HPV typing was done on cytology and CIN2/3 biopsies. If the whole-tissue section of the biopsy was positive for multiple hrHPV types, LCM-PCR was performed. We found 181 (70%) single and 71 (28%) multiple hrHPV infections in cytology, with 5 (2%) cases HPV-positive only on whole-tissue section PCR. Of cases with multiple cytologic hrHPV infections, 47/71 (66%) showed a single type in CIN2/3 lesions. In total, in 232 of 257 (90%) @entity1 with CIN2/3, a single hrHPV type caused CIN2/3. One was nonattributable on the LCM level. The remaining 24 @entity1 had 2 or more contiguous or separated lesions, each associated with a single hrHPV infection. The probability of @entity1799 being present in CIN2/3, if detected in cytology, was 0.96 (95% confidence interval=0.90-0.98). LCM-PCR confirms that only 9% of histologic CIN2/3 is associated with multiple hrHPV types, much less than cytology would indicate, and each lesion was associated with a single hrHPV infection.

Attributing Oncogenic XXXX Genotypes to High-grade Cervical Neoplasia: Which Type Causes the Lesion?

@entity1 :: ('9606', 'Species') :: ['women'] @entity1799 :: ('333760', 'Species') :: ['HPV16'] @entity1082 :: ('10566', 'Species') :: ['Human papillomavirus (HPV'] @entity3146 :: ('MESH:D018290', 'Disease') :: ['cervical intraepithelial neoplasia', 'CIN']
@entity1082 :: (10566,Species) :: ['Human Papillomavirus']
