OBJECTIVE: To study expression and function of the chemokine receptor @entity5075 in synovial fluid (SF) T cells from @entity1 with @entity309 ( @entity309 ). METHODS: Expression of @entity5075 was studied by flow cytometry and immunoblotting. The chemotactic response of T cells to chemokines was studied in cell migration assay. @entity669 phosphorylation of @entity25888 was evaluated in immunoprecipitation and immunoblotting. RESULTS: SF T cells showed an increase in the population of @entity5075 , @entity436 , and CD45RO positive cells and exhibited an increase in chemotactic activity, which was not augmented with @entity5737 but stromal cell-derived factor-1alpha. @entity669 phosphorylation per @entity25888 molecule was markedly enhanced in SF T cells. In H9 cells, @entity669 phosphorylation of not only focal adhesion kinase but also @entity25888 was induced after treatment with @entity5737 . Downmodulation of @entity5075 by @entity5737 was decreased and recycling of @entity5075 was accelerated in SF T cells when compared with peripheral blood (PB) T cells. When CD45RO positive PB T cells were cultured with @entity1199 , blunted responsiveness to @entity5737 -induced @entity6722 was reproduced as well as spontaneous @entity6722 , increased expression of @entity5075 , and aberrant receptor dynamics, after @entity5737 stimulation as observed in SF T cells. CONCLUSION: Synovial fluid T cells highly positive for @entity5075 show aberrant characteristics; resistant to @entity5737 in terms of migration, but responsive in terms of dynamics of @entity5075 .

Aberrant responsiveness to @entity5737 in synovial fluid T cells from XXXX with @entity309 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity6722 :: ('MESH:C565534', 'Disease') :: ['chemotaxis'] @entity5737 :: ('6352', 'Gene') :: ['RANTES'] @entity5075 :: ('1234', 'Gene') :: ['CCR5'] @entity1199 :: ('3558', 'Gene') :: ['interleukin 2'] @entity25888 :: ('4739', 'Gene') :: ['Crk-associated substrate lymphocyte-type (CasL)', 'CasL'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['Tyrosine', 'tyrosine'] @entity309 :: ('MESH:D001172', 'Disease') :: ['rheumatoid arthritis', 'RA'] @entity436 :: ('7852', 'Gene') :: ['CXCR4']
@entity1 :: (9606,Species) :: ['patients']
