@entity19341 ( @entity19341 ) represses transcription of certain genes and is encoded by a 3.0-kilobase mRNA (Kageyama, R., and Pastan, I. (1989) Cell 59, 815-825). The @entity19341 cDNA hybridizes to two additional mRNA species, 4.2 and 1.2 kilobases. We have used differential hybridization to identify a cDNA clone (termed @entity18675 ) for the 4. 2-kilobase mRNA and find that it is highly expressed in @entity1 cells. The open reading frame consists of 2256 nucleotides and encodes a protein of 752 @entity1522 with a calculated molecular mass of 83 kilodaltons. @entity18675 expressed in vitro using reticulocyte lysates and @entity526 migrates as a 160-kilodalton protein in @entity214 - @entity2975 gel electrophoresis but has a molecular mass of 83 kilodaltons as determined by mass spectrum analysis. @entity18675 binds to @entity470 promoter fragments, and the major binding site is located between @entity2036 -249 and -233. Cotransfection assays show that @entity18675 acts to repress transcription from the @entity470 promoter in constructs containing the major @entity18675 binding site and not when the site had been mutated. Thus, @entity18675 is a newly identified transcriptional repressor with aberrant electrophoretic mobility.

Molecular cloning and characterization of a transcription regulator with homology to XXXX .

@entity1 :: ('9606', 'Species') :: ['HUT-102'] @entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity2036 :: ('MESH:D009711', 'Chemical') :: ['nucleotides'] @entity470 :: ('1956', 'Gene') :: ['epidermal growth factor receptor'] @entity18675 :: ('9208', 'Gene') :: ['GCF2'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity19341 :: ('6936', 'Gene') :: ['GC-binding factor', 'GCF'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS'] @entity526 :: ('562', 'Species') :: ['Escherichia coli']
@entity19341 :: (6936,Gene) :: ['GC-binding factor']
