Several endophytic fungal strains from Srikaya plants (Annona squamosa L.) have been isolated and one of them was identified as Penicillium sp. Penicillium has been proven as an established source for a wide array of unique bioactive secondary metabolites that exhibit a variety of biological activities. The aim of this study is isolation of secondary metabolite from Penicillium, an endophytic of A. squamosa L. Penicillium sp. from endophytic of A. squamosa L. was fermented in Wicherham media. The whole extract from both liquid media and mycelium was partitioned by @entity1046 and evaporated to obtain crude @entity1046 extract. The @entity1046 extract was then brokedown using column chromatography with @entity3440 as stationary phase and mixture of @entity1046 / @entity1042 (98%:2%) as mobile phase and then was separated by sephadex column. Structure elucidation of isolated compounds were mainly done by analysis of one and two dimensional NMR (Nuclear Magnetic Resonance) data and supported by HPLC (High performance Liquid Chromatography) and @entity3928 ( @entity3928 ). Isolated secondary metabolites were tested using in vitro assays for anticancer and antimicrobial activity. For anticancer activity, the metabolites were tested against @entity0 cells (MCF-7) using @entity98 assay, while for antimicrobial activity was performed using disk diffusion assays. From these physical, chemical and spectral evidences that the secondary metabolites were confirmed as @entity69644 and @entity69645 . @entity69644 and @entity69645 have no activity as anticancer and antimicrobial.

Alkaloid ( @entity69645 and XXXX ) from endophytic fungi (Penicillium sp.) of Annona squamosa L.

@entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity1046 :: ('MESH:C007650', 'Chemical') :: ['ethyl acetate'] @entity1042 :: ('MESH:D000432', 'Chemical') :: ['methanol'] @entity3928 :: ('MESH:C536030', 'Disease') :: ['Mass Spectrometer-Time of Flight', 'MS-TOF'] @entity69645 :: ('CHEBI:70399', 'Chemical') :: ['Meleagrine'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity3440 :: ('MESH:D012822', 'Chemical') :: ['silica'] @entity69644 :: ('MESH:C005537', 'Chemical') :: ['Chrysogine']
@entity69644 :: (MESH:C005537,Chemical) :: ['Chrysogine']
