BACKGROUND: @entity3658 ( @entity3658 ) and its antagonist @entity877 ( @entity877 ) act on the endothelial cell @entity878 receptor to regulate vascular integrity and remodeling. The local balance of these factors and the level of other angiogenic factors determine whether blood vessels grow, are maintained or regress. Profound angiogenesis and vascular remodeling occur in the placenta and this is altered in @entity1468 , a major cause of maternal and fetal morbidity and mortality. MATERIALS AND METHODS: The mRNAs encoding @entity3658 , @entity877 , and @entity878 were detected and localized in @entity1 placentae throughout gestation. The mechanism of regulation angiopoietin mRNAs level was determined by explant culture in ambient and reduced @entity26 , and in the presence of @entity4316 . RESULTS: In situ hybridization showed that @entity877 mRNA was abundant in the syncytiotrophoblast in the first trimester of @entity1 pregnancy. @entity3658 mRNA could not be detected by in situ hybridization, but was by reverse transcriptase-polymerase chain reaction (RT-PCR) and Northern blotting. Placental vascular structure is altered in @entity1468 and intrauterine growth restriction, conditions where feto-placental oxygenation is perturbed. In villous explant cultures, a reduction in @entity26 tension significantly raised the levels of @entity877 mRNA, and this was dependent on transcription. However, similar experiments showed that the stability of the @entity3658 message was greatly reduced under these conditions. Thus, @entity519 has opposite effects on @entity3658 and @entity877 mRNA levels. Placentae obtained from @entity1 with @entity1468 had reduced levels of @entity877 mRNA compared to gestationally matched controls. There was no difference in the levels of @entity3658 mRNAs. CONCLUSIONS: These data show that the relative levels of @entity3658 and @entity877 mRNA are regulated by local @entity26 tension by different mechanisms and that this may be important during normal @entity1 placentation.

The regulation and localization of angiopoietin-1, -2, and their receptor @entity878 in normal and pathologic XXXX placentae.

@entity1 :: ('9606', 'Species') :: ['women', 'human'] @entity878 :: ('7010', 'Gene') :: ['Tie-2'] @entity3658 :: ('284', 'Gene') :: ['Ang-1', 'Angiopoietin-1'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity4316 :: ('MESH:D003609', 'Chemical') :: ['actinomycin D'] @entity519 :: ('MESH:D000860', 'Disease') :: ['hypoxia'] @entity1468 :: ('MESH:D011225', 'Disease') :: ['preeclampsia'] @entity877 :: ('285', 'Gene') :: ['angiopoietin-2', 'Ang-2']
@entity1 :: (9606,Species) :: ['human']
