UNASSIGNED: Early @entity75 are thought to be the initial findings of fibrogenesis in @entity16058 , but little is known about their properties. Type IV collagen comprises six gene products, a1-a6, and although it is known as a major basement membrane component, its abnormal deposition is seen in @entity75 of certain organs. We studied the expression of type I and III collagen and all a chains of type IV collagen in lung specimens from @entity1 with @entity2416 ( @entity2416 ) or @entity3616 ( @entity3616 ) via immunohistochemistry. With cultured lung fibroblasts, we analyzed the expression and function of all a chains of type IV collagen via immunohistochemistry, western blotting, real-time quantitative PCR, and a Boyden chamber migration assay after the knockdown of a1 and a2 chains. Although we observed @entity1413 in early @entity75 of both @entity2416 and @entity3616 , we found type IV collagen, especially a1 and a2 chains, in early @entity75 of @entity2416 but not @entity3616 . Fibroblasts enhanced the expression of a1 and a2 chains of type IV collagen after transforming growth factor-b1 stimulation. Small interfering RNA against a1 and a2 chains increased fibroblast migration, with upregulated phosphorylation of @entity3818 ( @entity3818 ), and adding medium containing fibroblast-produced a1 and a2 chains reduced the increased levels of fibroblast migration and phosphorylation of @entity3818 . Fibroblasts in @entity3616 were positive for phosphorylated @entity3818 but fibroblasts in @entity2416 were not. These results suggest that fibroblasts in @entity2416 with type IV collagen deposition, especially a1 and a2 chains, have less ability to migrate from early @entity75 than fibroblasts in @entity3616 without type IV collagen deposition. Thus, type IV collagen deposition in early @entity75 of @entity2416 may be implicated in refractory pathophysiology including @entity75 fibroblasts via a @entity3818 pathway.Laboratory Investigation advance online publication, 25 May 2015; doi:10.1038/labinvest.2015.66.

Role of a1 and a2 chains of type IV collagen in early XXXX of @entity16058 and migration of lung fibroblasts.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity16058 :: ('MESH:D054988', 'Disease') :: ['idiopathic interstitial pneumonias'] @entity75 :: ('MESH:D001927', 'Disease') :: ['migration of lesion', 'fibrotic lesions'] @entity3616 :: ('MESH:D018549', 'Disease') :: ['organizing pneumonia', 'OP'] @entity2416 :: ('MESH:D054990', 'Disease') :: ['UIP', 'usual interstitial pneumonia'] @entity3818 :: ('5747', 'Gene') :: ['FAK', 'focal adhesion kinase'] @entity1413 :: ('MESH:D003095', 'Disease') :: ['type I and III collagens']
@entity75 :: (MESH:D001927,Disease) :: ['fibrotic lesions']
