Elucidating the role of viral genes in transgenic plants revealed that the movement protein (MP) from @entity21104 is responsible for altered @entity1735 allocation in @entity286 and @entity415 plants. To study whether this is a general feature of viral MPs, the movement protein MP17 of @entity64370 ( @entity64370 ), a phloem-restricted luteovirus, was constitutively expressed in @entity286 plants. Transgenic lines were strongly reduced in height and developed bleached and sometimes even necrotic areas on their source leaves. Levels of soluble @entity3363 and starch were significantly increased in source leaves. Yet, in leaf laminae the @entity60327 content was unaltered and @entity855 reduced to only a small extent, indicating that these leaves were able to maintain homeostatic conditions by compartmentalization of soluble @entity3363 , probably in the vacuole. On the contrary, midribs contained lower levels of soluble @entity3363 , @entity855 , @entity20251 and @entity858 - @entity413 supporting the concept of limited uptake and catabolism of @entity969 in the phloem. The accumulation of @entity3363 led to a decreased photosynthetic capacity and carboxylation efficiency of @entity9578 carboxylase/oxygenase (rubisco) probably owing to decreased expression of photosynthetic proteins. In parallel, levels of pathogenesis-related proteins were elevated which may be the reason for the obtained limited resistance against the unrelated @entity39429 (PVY)N in the transgenic @entity286 plants. Ultrathin sections of affected leaves harvested from 2-week-old plants revealed plasmodesmal alterations in the phloem tissue while plasmodesmata between mesophyll cells were indistinguishable from wild-type. These data favour the phloem tissue to be the primary site of @entity64370 MP17 action in altering @entity1735 metabolism.

Expression of a luteoviral movement protein in transgenic plants leads to XXXX accumulation and reduced photosynthetic capacity in source leaves.

@entity286 :: ('4097', 'Species') :: ['tobacco'] @entity21104 :: ('12242', 'Species') :: ['tobacco mosaic virus'] @entity64370 :: ('12045', 'Species') :: ['PLRV', 'potato leafroll virus'] @entity858 :: ('MESH:D014530', 'Chemical') :: ['UDP'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['carbohydrate'] @entity20251 :: ('MESH:D006600', 'Chemical') :: ['hexose-phosphates'] @entity415 :: ('4113', 'Species') :: ['potato'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity60327 :: ('CHEBI:47878', 'Chemical') :: ['hexose-phosphate'] @entity9578 :: ('MESH:C001933', 'Chemical') :: ['ribulose-1,5-bisphosphate'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity39429 :: ('12216', 'Species') :: ['potato virus Y'] @entity3363 :: ('MESH:D002241', 'Chemical') :: ['sugars', 'carbohydrates'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity1735 :: (CHEBI:16646,Chemical) :: ['carbohydrate']
