UNASSIGNED: Long non-coding RNAs (lncRNAs) are thought to have various functions other than RNA silencing. We tried to evaluate the expression of lncRNAs in @entity1 with @entity785 ( @entity785 ) and determined whether lncRNAs controls collagen expression in dermal fibroblasts. lncRNA expression was determined by real-time PCR and in situ hybridization. Protein and mRNA levels of collagen were analysed using immunoblotting and real-time PCR. We found @entity68597 , one of the lncRNAs, was overexpressed in @entity785 dermal fibroblasts both in vivo and in vitro, which was inhibited by the transfection of transforming growth factor (TGF)-b1 siRNA. @entity68597 siRNA reduced the mRNA expression of type I collagen in normal and @entity785 dermal fibroblasts, but not the levels of major disease-related cytokines. In addition, @entity68597 siRNA significantly reduced type I collagen mRNA stability, but not protein half-lives. Furthermore, we first investigated serum lncRNA levels in @entity1 with @entity785 , and serum @entity68597 levels were significantly increased in @entity785 @entity1 . @entity68597 is a new regulator of collagen expression which stabilizes the collagen mRNA. The upregulation of @entity68597 seen in @entity785 fibroblasts may result from activated endogenous TGF-b signalling and may play a role in the constitutive upregulation of collagen in these cells. Further studies on the regulatory mechanism of tissue @entity82 by lncRNAs in @entity785 skin lead to a better understanding of the pathogenesis, new diagnostic methods by their serum levels and new therapeutic approaches using siRNAs.

Long non-coding RNA XXXX is upregulated in scleroderma dermal fibroblasts and controls collagen mRNA stabilization.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis'] @entity68597 :: ('9383', 'Gene') :: ['TSIX'] @entity785 :: ('MESH:D012595', 'Disease') :: ['systemic sclerosis', 'SSc']
@entity68597 :: (9383,Gene) :: ['TSIX']
