UNASSIGNED: Almost all synthetic pathways for biofuel production are designed to require endogenous metabolites in glycolysis, such as @entity7954 , @entity2388 , and @entity6625 . However, such metabolites are also required for bacterial cell growth. To reduce the metabolic imbalance between cell growth and target chemical production, we previously constructed a metabolic toggle switch (MTS) as a conditional flux redirection tool controlling metabolic flux of @entity2974 cycle toward @entity5723 production. This approach succeeded to improve the @entity5723 production titer and yield while ensuring sufficient cell growth. However, excess accumulation of @entity2388 , the precursor for @entity6625 synthesis, was also observed. In this study, for efficient conversation of @entity2388 to @entity6625 ( @entity2388 oxidation), we designed a synthetic metabolic bypass composed of poxB and acs with the MTS for @entity6625 supply from the excess @entity2388 . When this designed bypass was expressed at the appropriate expression level associated with the conditional metabolic flux redirection, @entity2388 accumulation was prevented, and the @entity5723 production titer and yield were improved. Final @entity5723 production titer of strain harboring MTS with the synthetic metabolic bypass improved 4.4-fold compared with strain without metabolic flux regulation, and it was 1.3-fold higher than that of strain harboring the conventional MTS alone. Additionally, @entity413 consumption was also improved 1.7-fold compared with strain without metabolic flux regulation. On the other hand, introduction of the synthetic metabolic bypass alone showed no improvement in @entity5723 production and @entity413 consumption. These results showed that the improvement in bio-production process caused by synergy between the MTS and the synthetic metabolic bypass.

Synthetic metabolic bypass for a metabolic toggle switch enhances XXXX supply for @entity5723 production by Escherichia coli.

@entity7954 :: ('MESH:D010728', 'Chemical') :: ['phosphoenolpyruvate'] @entity5723 :: ('MESH:D019840', 'Chemical') :: ['isopropanol'] @entity2974 :: ('CHEBI:30956', 'Chemical') :: ['TCA'] @entity6625 :: ('MESH:D000105', 'Chemical') :: ['acetyl-CoA'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity2388 :: ('MESH:D019289', 'Chemical') :: ['pyruvate']
@entity6625 :: (MESH:D000105,Chemical) :: ['acetyl-CoA']
