UNASSIGNED: Soil microorganisms are key contributors to nutrient cycling and are essential for the maintenance of healthy soils and sustainable agriculture. Although the antimicrobial effects of a broad range of nanoparticulate substances have been characterised in vitro, little is known about the impact of these compounds on microbial communities in environments such as soil. In this study, the effect of three widely used nanoparticulates ( @entity575 , @entity3440 and @entity44913 ) on @entity364 in an agricultural pastureland soil was examined in a microcosm-based experiment using a combination of enzyme analysis, molecular fingerprinting and amplicon sequencing. A relatively low concentration of @entity575 nanoparticles (AgNPs) significantly reduced total soil dehydrogenase and urease activity, while @entity16773 and @entity3758 nanoparticles had no effect. Amplicon sequencing revealed substantial shifts in bacterial community composition in soils amended with AgNPs, with significant decreases in the relative abundance of Acidobacteria and Verrucomicrobia and an increase in Proteobacteria. In particular, the relative abundance of the @entity9497 significantly increased in AgNP amended soil. The effects of @entity16773 and @entity3758 NPs on bacterial community composition were less pronounced. AgNPs significantly reduced bacterial and archaeal amoA gene abundance in soil, with the archaea more susceptible than bacteria. AgNPs also significantly impacted soil fungal community structure, while @entity16773 and @entity3758 NPs had no effect. Several fungal ribotypes increased in soil amended with AgNPs, compared to control soil. This study highlights the need to consider the effects of individual nanoparticles on soil microbial communities when assessing their environmental impact.

Soil microbial community responses to contamination with @entity575 , XXXX and @entity3440 nanoparticles.

@entity16773 :: ('CHEBI:30192', 'Chemical') :: ['Al2O3'] @entity575 :: ('MESH:D012834', 'Chemical') :: ['silver'] @entity3758 :: ('CHEBI:30563', 'Chemical') :: ['SiO2'] @entity44913 :: ('CHEBI:30187', 'Chemical') :: ['aluminium oxide'] @entity364 :: ('MESH:D009181', 'Disease') :: ['bacterial and fungal communities'] @entity3440 :: ('MESH:D012822', 'Chemical') :: ['silicon dioxide'] @entity9497 :: ('MESH:D056305', 'Disease') :: ['Proteobacterial genus Dyella']
@entity44913 :: (CHEBI:30187,Chemical) :: ['aluminium oxide']
