UNASSIGNED: A sensitive and reliable @entity15 /MS method was developed and validated for the determination of free (unconjugated), glucuronidated, sulfated, and total (free and conjugated) HDND-7 in @entity35 plasma and tissues. Plasma and tissues samples were treated prior to and after the enzyme hydrolysis. @entity1172 was achieved on a Phenomenex Luna C18 column (150*4.6mm, 3 m), using isocratic mobile phase consisting of 0.1% @entity5189 - @entity1626 (50:50, v/v) at a flow rate of 300 l/min. The detection was performed on a triple quadruple tandem mass spectrometer using positive electrospray ionization (ESI) source with a chromatographic run time of 5.0min. The detection was operated by multiple reaction monitoring (MRM) of the transitions of m/z 429.3 > 223.9 for HDND-7 and 272.9 > 152.9 for @entity21119 (IS), respectively. This method was validated in terms of specificity, linearity, precision, accuracy, and stability. The calibration curves for plasma and tissues were linear over a wide concentration range of 0.02-40 g/ml with a lower limit of quantification (LLOQ) of 0.02 g/ml. Mean extraction recoveries in plasma and tissues ranged from 87.4 to 97.1% and from 54.2 to 70.5%, respectively. The intra- and inter-day precision values were below 15% and the accuracy was within   15%. The samples were stable under all the tested conditions. This method has been successfully applied to the pharmacokinetic study following oral doses of 25, 50 and 100mg/kg and intravenous dose of 25mg/kg, and tissue distribution study following oral dose of 50mg/kg.

A XXXX /MS method for the quantitation of free, conjugated, and total HDND-7, a novel hesperetin derivative, in @entity35 plasma and tissues: Application to the pharmacokinetic and tissue distribution study.

@entity21119 :: ('MESH:C005273', 'Chemical') :: ['naringenin'] @entity1626 :: ('MESH:C032159', 'Chemical') :: ['acetonitrile'] @entity1172 :: ('MESH:D001010', 'Disease') :: ['Chromatographic separation'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity15 :: ('MESH:D009103', 'Disease') :: ['HPLC-MS'] @entity5189 :: ('MESH:C030544', 'Chemical') :: ['formic acid']
@entity15 :: (MESH:D009103,Disease) :: ['HPLC-MS']
