PURPOSE: Reactive @entity26 species, which are primarily produced by leukocytes, are generally detrimental to sperm. High reactive @entity26 species levels are found in @entity1 with abnormal sperm function. Since @entity1 often have poor sperm characteristics and @entity249 after vasectomy reversal, we compared reactive @entity26 species in seminal cells of @entity1 after vasovasostomy to those of fertile @entity1 to determine if reactive @entity26 species were elevated in the former group. MATERIALS AND METHODS: We studied semen samples of @entity1 with proved fertility (39) and those with previously proved fertility who had undergone vasectomy reversal (45). The presence of leukocytes was determined by Bryan-Leishman staining. Reactive @entity26 species endogenous activity was monitored by @entity6931 dependent chemiluminescence in washed cells, including all cells in the semen, and Percoll density gradient purified sperm. RESULTS: After vasovasostomy @entity1 had significantly lower sperm concentration, motility and computerized motility measurements than fertile @entity1 . Mean reactive @entity26 species in washed seminal cells after vasovasostomy was 684 relative light units per second compared to 49 for fertile controls (p < 0.0001). Density gradient purified sperm had 53 and 0.64 relative light units per second, respectively (p < 0.0001). When @entity1 with leukocytospermia were excluded from analysis, differences between the groups remained, although 9 times more reactive @entity26 species were detected in @entity1 after vasectomy reversal with than those without leukocytes in semen. CONCLUSIONS: Higher levels of reactive @entity26 species are found in washed seminal cells and purified sperm after vasectomy reversal than in those of fertile @entity1 . Although leukocytes are probably a significant source of reactive @entity26 species in these groups, they may not account for all of the increased reactive @entity26 species after vasovasostomy. Low motility after vasectomy reversal may be related to the detrimental effects of reactive @entity26 species produced by leukocytes or sperm, even in @entity1 without clinical leukocytospermia.

Vasectomy reversal associated with increased reactive XXXX species production by seminal fluid leukocytes and sperm.

@entity1 :: ('9606', 'Species') :: ['men'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity6931 :: ('MESH:D008165', 'Chemical') :: ['luminol'] @entity249 :: ('MESH:D007246', 'Disease') :: ['infertility']
@entity26 :: (MESH:D010100,Chemical) :: ['oxygen']
