INTRODUCTION: In the context of drug delivery, mesenchymal stromal cells (MSCs) from bone marrow and adipose tissue have emerged as interesting candidates due to their homing abilities and capacity to carry toxic loads, while at the same time being highly resistant to the toxic effects. Amongst the many sources of MSCs which have been identified, the @entity1 term placenta has attracted particular interest due to its unique, tissue-related characteristics, including its high cell yield and virtually absent expression of human leukocyte antigens and co-stimulatory molecules. Under basal, non-stimulatory conditions, placental MSCs also possess basic characteristics common to MSCs from other sources. These include the ability to secrete factors which promote cell growth and tissue repair, as well as immunomodulatory properties. The aim of this study was to investigate MSCs isolated from the amniotic membrane of @entity1 term placenta (hAMSCs) as candidates for drug delivery in vitro. METHODS: We primed hAMSCs from seven different donors with @entity1311 ( @entity4045 ) and investigated their ability to resist the cytotoxic effects of @entity4045 , to upload the drug, and to release it over time. We then analyzed whether the uptake and release of @entity4045 was sufficient to inhibit proliferation of CFPAC-1, a @entity788 cell line sensitive to @entity4045 . RESULTS: For the first time, our study shows that hAMSCs are highly resistant to @entity4045 and are not only able to uptake the drug, but also release it over time. Moreover, we show that @entity4045 is released from hAMSCs in a sufficient amount to inhibit @entity5 cell proliferation, whilst some of the @entity4045 is also retained within the cells. CONCLUSION: Taken together, for the first time our results show that placental stem cells can be used as vehicles for the delivery of cytotoxic agents.

XXXX amniotic mesenchymal stromal cells (hAMSCs) as potential vehicles for drug delivery in @entity5 therapy: an in vitro study.

@entity1 :: ('9606', 'Species') :: ['human'] @entity788 :: ('MESH:D010190', 'Disease') :: ['pancreatic tumor'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity1311 :: ('MESH:D017239', 'Chemical') :: ['paclitaxel'] @entity4045 :: ('D010852', 'Chemical') :: ['PTX']
@entity1 :: (9606,Species) :: ['Human']
