To investigate the susceptibilities of fungal and @entity1 cells to @entity2379 ( @entity11015 ), @entity11015 -loaded @entity6579 (LPC)micelles as drug delivery vehicles were incubated at 37 degrees C with @entity2434 vesicles containing different @entity3150 as model systems for fungal and @entity1 cells. The binding and kinetics of @entity11015 to @entity3150 in the membranes were judged by UV-visible spectroscopy. In the 91% monomeric form, @entity11015 interacted rapidly with @entity12364 and slowly with @entity14952 ( @entity14952 ), while it did not interact with @entity165 . In the 50% monomeric form, @entity11015 formed complexes more rapidly with @entity12364 or @entity14952 than in the monomeric form, whereas it did not still interact with @entity165 . The interaction was also characterized by resonance energy transfer between the fluorescent probe @entity27956 ( @entity27956 ) and @entity11015 . In the 91% monomeric form, @entity11015 caused initial fluorescence quenching in bilayer membranes containing any @entity5068 as well as @entity5068 -free bilayer membranes due to the release of @entity11015 and its incorporation within the membranes. However, a second phase of increasing fluorescence was found in the case of @entity12364 alone. On the other hand, in the 47% monomeric form, @entity11015 gave a biphasic intensity profile in membranes containing any @entity5068 as well as @entity5068 -free membranes. However, the extent of the second phase of increasing fluorescence intensity was markedly dependent upon @entity5068 composition. Studies using @entity5068 -containing vesicles provide important insights into the role of the aggregation state of @entity11015 in its effects on cells.

Susceptibilities of phospholipid vesicles containing different @entity3150 to XXXX -loaded lysophosphatidylcholine micelles.

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity12364 :: ('MESH:D004875', 'Chemical') :: ['ergosterol'] @entity6579 :: ('MESH:D008244', 'Chemical') :: ['lysophosphatidylcholine'] @entity2379 :: ('MESH:D000666', 'Chemical') :: ['amphotericin B'] @entity14952 :: ('MESH:C016705', 'Chemical') :: ['7-dehydrocholesterol', '7-DHC'] @entity2434 :: ('MESH:D010713', 'Chemical') :: ['phosphatidylcholine'] @entity3150 :: ('MESH:D013261', 'Chemical') :: ['sterols'] @entity165 :: ('MESH:D002784', 'Chemical') :: ['cholesterol'] @entity27956 :: ('MESH:C033628', 'Chemical') :: ['trimethylammonium diphenylhexatriene', 'TMA-DPH'] @entity5068 :: ('CHEBI:15889', 'Chemical') :: ['sterol'] @entity11015 :: ('CHEBI:40719', 'Chemical') :: ['AmB']
@entity2379 :: (MESH:D000666,Chemical) :: ['amphotericin B']
