BACKGROUND: @entity79 ( @entity79 ) is used for bone healing due to its osteoinductivity, but it requires a carrier for clinical application. Here, we report the effects on the osteoinductivity of @entity79 by use of a @entity12185 -based hydrogel as the carrier, compared to sterile water. METHODS: @entity79 -W and @entity79 -H represent 27 wt% of @entity79 with sterile water and @entity79 with a @entity12185 -based hydrogel, respectively. Both of the compositions were applied to @entity1 mesenchymal stem cell (MSC) cultures, and monitored for @entity623 ( @entity623 ) staining and @entity623 activity. Six 10-week-old athymic nude @entity35 were used for abdominal muscle grafting with either @entity79 -W or @entity79 -H, and were tested by plane radiography, microfocus X-ray computed tomography (CT), and decalcified histology to evaluate ectopic bone formation. RESULTS: The @entity79 -W group showed stronger @entity623 staining at 7, 14, and 21 days of treatment, and significantly higher @entity623 activity at 7 and 14 days of treatment, compared to the @entity79 -H group. Plane radiography could not confirm the radio-opaque lesions in the @entity35 ectopic bone formulation model. However, ectopic bone formation was observed in both groups by micro-CT. Compared to the @entity79 -H group, the @entity79 -W group showed higher bone volume, percent bone volume and trabecular number, and the difference in percent bone volume was statistically significant. Decalcified histology found bony tissue with lamellation in both groups. CONCLUSIONS: Our results suggest that @entity12185 -based hydrogel has efficacy as a @entity79 carrier since it shows ectopic bone formation, but its effects on the quality and quantity of osteoblastic differentiation in @entity35 abdominal ectopic bone and MSC are considered negative.

The effect of XXXX -based hydrogel on the osteoinductivity of demineralized bone matrix.

@entity1 :: ('9606', 'Species') :: ['human'] @entity79 :: ('MESH:D001847', 'Disease') :: ['Demineralized bone matrix', 'DBM'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity623 :: ('250', 'Gene') :: ['ALP', 'alkaline phosphatase'] @entity12185 :: ('MESH:D020442', 'Chemical') :: ['poloxamer 407']
@entity12185 :: (MESH:D020442,Chemical) :: ['poloxamer 407']
