OBJECTIVES: The activity of membrane-bound @entity12359 and soluble e-Ns and @entity34857 5'-nucleotidases was evaluated on lymphocytes from @entity1 affected by @entity1072 ( @entity1072 ). A statistically significative decrease in @entity12359 , e-Ns, and @entity34857 activities was observed in peripheral blood lymphocytes and in B and T populations from affected individuals. DESIGN AND METHODS: For the assay of @entity12359 , e-Ns, and @entity34857 activity we used a radioactive procedure coupled to HPLC. Since the @entity12359 is identified as @entity12359 antigen, we performed immunofluorescence analysis using a specific monoclonal antibody. We analyzed @entity12359 mRNA by RT-PCR to ascertain the possibility of an alteration in the transcription of its gene. RESULTS: A decrease in @entity12359 activity was correlated to reduction in @entity12359 positive cells ( @entity12359 +) in @entity1778 @entity1 . RT-PCR produced a fragment of the expected size and the specific mRNA was found expressed in both healthy subjects and @entity1778 @entity1 . CONCLUSIONS: The decrease in @entity12359 activity in @entity1 with @entity1072 is not due to loss of transcription of the specific mRNA. The presence of point mutations, splicing alteration, or posttranslational modifications must be investigated. If a defect at DNA or RNA level will be detected, the molecular analysis will be considered for diagnosis of @entity1072 .

5'-nucleotidase activity in lymphocytes from @entity1 affected by XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity34857 :: ('22978', 'Gene') :: ['c-N-II'] @entity1072 :: ('MESH:D015451', 'Disease') :: ['B-cell chronic lymphocytic leukemia', 'B-CLL'] @entity1778 :: ('MESH:D007938', 'Disease') :: ['leukemia'] @entity12359 :: ('4907', 'Gene') :: ["ecto-5'-nucleotidase", 'CD73']
@entity1072 :: (MESH:D015451,Disease) :: ['B-cell chronic lymphocytic leukemia']
