We have previously demonstrated that fresh or cryopreserved xenogeneic hepatocytes manually macroencapsulated in @entity8606 polymer and transplanted intraperitoneally in @entity35 were able to improve the survival rate after 95% hepatectomy without immunosuppression. In addition, we developed a semiautomatic device where porcine hepatocytes were coextruded with @entity8606 hydrogel in order to macroencapsulate large amounts of cells. The purpose of the present study was to 1) test whether transplanted porcine hepatocytes macroencapsulated in this device remained functional as evaluated by their ability to prevent @entity204 from @entity879 , and 2) compare the efficiency of cryopreserved or freshly isolated hepatocytes. Fresh or cryopreserved porcine hepatocytes were macroencapsulated in the semiautomatic device by coextrusion in @entity8606 polymer in 2-m minitubes containing 6 x 10(7) cells. @entity879 was induced in @entity35 by two-step 95% hepatectomy. At the time of completion of liver resection, @entity35 were either not transplanted with minitubes (control group I, n = 13), or were implanted with two minitubes containing culture medium (control group II, n = 11), hepatocytes killed by heat treatment (control group III, n = 10), coextruded fresh hepatocytes (group IV, n = 11), or coextruded cryopreserved hepatocytes (group V, n = 11), without immunosuppression. The survival rate at day 7 was between 0% and 31% in the three control groups. By contrast, coextruded fresh hepatocytes significantly improved the survival rate (group IV, 82%) as did cryopreserved cells (group V, 91% survival). In surviving @entity35 , minitubes were explanted after 20 days; either fresh or cryopreserved hepatocytes appeared morphologically viable and their ultrastructure was preserved. Their detoxification capacities evaluated by the activity of the cyt P450 CYP3A4 were partly maintained. In conclusion, porcine hepatocytes macroencapsulated by coextrusion using a semiautomatic device and transplanted without immunosuppression were able to prevent @entity204 from @entity879 in @entity35 . Cryopreserved cells were as efficient as fresh hepatocytes.

Semiautomatic macroencapsulation of fresh or cryopreserved porcine hepatocytes maintain their ability for treatment of XXXX .

@entity8606 :: ('MESH:C016892', 'Chemical') :: ['AN69'] @entity879 :: ('MESH:D017114', 'Disease') :: ['acute liver failure', 'Acute liver failure'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death'] @entity35 :: ('10116', 'Species') :: ['rats']
@entity879 :: (MESH:D017114,Disease) :: ['acute liver failure']
