@entity1 placenta is an organ which protects, feeds, and regulates the grooving of the embryo. Therefore, identification and characterization of placental components including proteins and their multi-protein complexes is an important step to understanding the placenta function. We have obtained and analyzed for the first time an extremely stable multi-protein complex (SPC,    1000 kDa) from the soluble fraction of three @entity1 placentas. By gel filtration on Sepharose-4B, the SPC was well separated from other proteins of the placenta extract. Light scattering measurements and gel filtration showed that the SPC is stable in the presence of @entity1219 , @entity8723 , @entity1626 , @entity2097 , and @entity33534 in high concentrations, but dissociates efficiently in the presence of 8 M @entity1133 , 50 mM @entity1098 , and 0.5 M @entity1219 . Such a stable complex is unlikely to be a casual associate of different proteins. According to @entity214 -PAGE and MALDI mass spectrometry data, this complex contains many major glycosylated proteins with low and moderate molecular masses (MMs) 4-14 kDa and several moderately abundant (79.3, 68.5, 52.8, and 27.2 kDa) as well as minor proteins with higher MMs. The SPC treatment with @entity3902 led to a disappearance of some protein bands and revealed proteins with lower MMs. The SPCs from three placentas efficiently hydrolyzed plasmid supercoiled DNA with comparable rates and possess at least two DNA-binding sites with different affinities for a 12-mer oligonucleotide. Progress in study of placental protein complexes can promote understanding of their biological functions.

Extremely Stable Soluble High Molecular Mass Multi-Protein Complex with DNase Activity in XXXX Placental Tissue.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity1133 :: ('MESH:D014508', 'Chemical') :: ['urea'] @entity1219 :: ('CHEBI:26710', 'Chemical') :: ['NaCl'] @entity3902 :: ('MESH:D004229', 'Chemical') :: ['dithiothreitol'] @entity8723 :: ('MESH:D015636', 'Chemical') :: ['MgCl2'] @entity1626 :: ('MESH:C032159', 'Chemical') :: ['acetonitrile'] @entity33534 :: ('CHEBI:29234', 'Chemical') :: ['Triton'] @entity2097 :: ('MESH:D019791', 'Chemical') :: ['guanidinium chloride'] @entity1098 :: ('MESH:D004492', 'Chemical') :: ['EDTA'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS']
@entity1 :: (9606,Species) :: ['Human']
