OBJECTIVE: To study the role of tranilast in the pathogenesis of @entity82 in @entity2401 . METHODS: Seventy-two BALB/C @entity19 were randomly divided into control, model and intervention groups (n=24 each). @entity19 in the model and intervention groups were infected with Coxsackievirus B3 to induce @entity2401 . The intervention group was given with tranilast (200 mg/kg) by gavage until sacrifice for sampling, while the other two groups were administered with the same volume of normal saline. Cardiac tissues were obtained from 8 @entity19 on 7, 14 and 28 days after modeling. The mast cell number was observed by @entity11979 staining and @entity32341 staining. The cardiac tissues were stained with @entity1710 and @entity1711 as well as masson trichrome to observe the pathological changes in cardiac tissues. The mRNA and protein expression of osteopontin and transforming growth factor-b1 was measured by RT-PCR and immunohistochemistry respectively. RESULTS: In the model group, the mRNA and protein expression of osteopontin reached the highest level on the 7th day, decreasing from the 14th day, and became to the least on the 28th day; while the expression of @entity641 increased from the 7th day, reaching a peak on the 14th day, and decreased slightly on the 28th day. The mRNA and protein expression of @entity641 and @entity1189 was lower in the intervention group than the model group (P<0.05), but higher than the control group (P<0.05). The expression of @entity1189 mRNA was positively correlated to the number of mast cells. CONCLUSIONS: Tranilast can reduce @entity296 by decreasing the number of mast cells, inhibiting the expression of @entity641 and @entity1189 .

[Effect of tranilast on XXXX in @entity19 with @entity2401 ].

@entity82 :: ('MESH:D005355', 'Disease') :: ['myocardiac fibrosis'] @entity296 :: ('MESH:D009202', 'Disease') :: ['myocardial fibrosis'] @entity19 :: ('10090', 'Species') :: ['mice', 'Mice'] @entity32341 :: ('MESH:C009469', 'Chemical') :: ['thionine'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity641 :: ('21803(Tax:10090)', 'Gene') :: ['TGF-b1'] @entity11979 :: ('MESH:D014048', 'Chemical') :: ['toluidine blue'] @entity1189 :: ('20750(Tax:10090)', 'Gene') :: ['OPN'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hematoxylin'] @entity2401 :: ('MESH:D009205', 'Disease') :: ['viral myocarditis']
@entity296 :: (MESH:D009202,Disease) :: ['myocardial fibrosis']
