UNASSIGNED: Inhibitory @entity1246 ( @entity1246 )-mediated neurotransmission plays an important role in the regulation of the prefrontal cortex (PFC), with increasing evidence suggesting that dysfunctional GABAergic processing of the PFC may underlie @entity51 reported across @entity914 . @entity888 ( @entity7865 ) is a psychostimulant that induces @entity716 in a subset of users, with repeat administration producing a progressively increased vulnerability to @entity716 following subsequent drug administration (sensitization). The aim here was to investigate changes to GABAergic mRNA expression in the PFC of @entity35 sensitized to @entity7865 using quantitative polymerase chain reaction (qPCR). Male @entity35 (n=12) underwent repeated @entity888 (intraperitoneal (i.p.) or saline injections for 7 days. Following 14 days of withdrawal, @entity35 were challenged with acute @entity888 (1mg/kg i.p.) and RNA was isolated from the PFC to compare the relative mRNA expression of a range of @entity1246 enzymes, transporters and receptors subunits. @entity7865 challenge resulted in a significant sensitized behavioral (locomotor) response in @entity7865 pre-treated animals compared with saline pre-treated controls. The mRNAs of transporters ( @entity44297 and @entity44299 ), ionotropic GABAA receptor subunits (a3 and b1), together with the metabotropic GABAB1 receptor, were upregulated in the PFC of sensitized @entity35 compared with saline controls. These findings indicate that GABAergic mRNA expression is significantly altered at the pre and postsynaptic level following sensitization to @entity7865 , with sensitization resulting in the transcriptional upregulation of several inhibitory genes. These changes likely have significant consequences on @entity1246 -mediated neurotransmission in the PFC and may underlie certain symptoms conserved across @entity914 , such as @entity409 .

GABAergic mRNA expression is upregulated in the prefrontal cortex of XXXX sensitized to @entity888 .

@entity44297 :: ('50676(Tax:10116)', 'Gene') :: ['GAT1'] @entity1246 :: ('MESH:D005680', 'Chemical') :: ['gamma-aminobutyric acid', 'GABA'] @entity888 :: ('MESH:D008694', 'Chemical') :: ['Methamphetamine', 'methamphetamine'] @entity7865 :: ('D008694', 'Chemical') :: ['METH'] @entity409 :: ('MESH:D008107', 'Disease') :: ['executive dysfunction'] @entity44299 :: ('79213(Tax:10116)', 'Gene') :: ['GAT3'] @entity914 :: ('MESH:D011618', 'Disease') :: ['psychotic disorders'] @entity51 :: ('MESH:D009461', 'Disease') :: ['certain deficits'] @entity716 :: ('MESH:D011605', 'Disease') :: ['psychotic relapse', 'chronic psychosis'] @entity35 :: ('10116', 'Species') :: ['Sprague-Dawley rats', 'rats']
@entity35 :: (10116,Species) :: ['rats']
