Phospholipase C (PLC) and internal @entity141 stores are involved in a variety of cellular functions. However, our understanding of PLC in @entity1 olfactory sensory neurons (OSNs) is generally limited to its controversial role in odor transduction. Here we employed single-cell @entity141 imaging and molecular approaches to investigate PLC-mediated @entity141 responses and its isozyme gene transcript expression. We found that the pan-PLC activator m-3M3FBS (25  M) induces intracellular @entity141 increases in vast majority of isolated @entity19 OSNs tested. Both the response amplitude and percent responding cells depend on m-3M3FBS concentrations. In contrast, the inactive analog o-3M3FBS fails to induce @entity141 responses. The m-3M3FBS-induced @entity141 increase is blocked by the PLC inhibitor @entity4385 , while its inactive analog U73433 has no effect. Removal of extracellular @entity141 does not change significantly the m-3M3FBS-induced @entity141 response amplitude. Additionally, in the absence of external @entity141 , we found that a subset of OSNs respond to an odorant mixture with small @entity141 increases, which are significantly suppressed by @entity4385 . Furthermore, using reverse transcription polymerase chain reaction and real-time quantitative polymerase chain reaction, we found that multiple PLC isozyme gene transcripts are expressed in olfactory turbinate tissue in various levels. Using RNA in situ hybridization analysis, we further show expression of @entity68374 gene transcripts in OSNs. Taken together, our results establish that PLC isozymes are potent enzymes for mobilizing intracellular @entity141 in @entity19 OSNs and provide molecular insight for PLC isozymes-mediated complex cell signaling and regulation in the peripheral olfactory epithelium.

Increases in intracellular calcium via activation of potentially multiple phospholipase C isozymes in XXXX olfactory neurons.

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity4385 :: ('MESH:C060229', 'Chemical') :: ['U73122'] @entity68374 :: ('21355;100073351', 'Gene') :: ['b4, y1, y2'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)']
@entity19 :: (10090,Species) :: ['mouse']
