OBJECTIVES: We studied collagen plugging of the fetoscopic access site in an in vivo fetal lamb model for fetoscopic surgery and possible role for @entity69514 and tissue inhibitors (TIMPs). METHODS: Eight ewes had @entity432 of the @entity825 as an experimental treatment for @entity2914 between days 88 and 99 of gestation (term 145 days) with sampling of amniotic, allantoic, and tracheal fluid. Nonoperated cotwins were used as controls. The fetoscopy port was closed using a collagen plug. Ten days (range 9-12) later, fluids were sampled and plug sites collected for histologic analysis. Activity of @entity31581 (72 kDa, gelatinase A) and @entity30676 (92 kDa, gelatinase B) was determined in the fluids by zymography and secretion of TIMPs (27-30 kDa; @entity31582 , glycosylated @entity69515 and @entity69516 , 24 kDa; unglycosylated @entity69515 , 21 kDa; @entity69517 ) by reverse zymography and quantified by densitometric analysis. RESULTS: No pregnancy was complicated by amniorhexis or @entity3152 . At cesarean, normal volumes of amniotic and allantoic fluid were present in all cases. Histology of the plug sites revealed good integration of the collagen plug without complete restoration of membrane integrity. @entity31581 , @entity30676 , and TIMPs were detected in all fluids. In the operated animals, significantly (P <.05) higher activity of @entity30676 was found in amniotic fluid, with lower concentrations of TIMPs in allantoic fluid (P <.01). @entity432 was associated with a significant (P <.02) increase in both @entity69514 in tracheal fluid. CONCLUSION: Collagen plugging of the fetoscopic access port sites in @entity742 resulted in functionally effective sealing of the fetal membranes. Changes in @entity31581 , @entity30676 , and TIMPs suggest an active remodeling of both the fetal lung and the fetal membranes.

Matrix metalloproteinases-2 and -9 and their endogenous tissue inhibitors in tissue remodeling after sealing of the fetal membranes in a XXXX model of fetoscopic surgery.

@entity825 :: ('MESH:D055090', 'Disease') :: ['trachea'] @entity30676 :: ('100192422(Tax:9940)', 'Gene') :: ['MMP-9'] @entity3152 :: ('MESH:D007752', 'Disease') :: ['preterm labor'] @entity69515 :: ('100217407(Tax:9940)', 'Gene') :: ['TIMP-3'] @entity31582 :: ('443331(Tax:9940)', 'Gene') :: ['TIMP-1'] @entity69517 :: ('100217406(Tax:9940)', 'Gene') :: ['TIMP-2'] @entity742 :: ('9940', 'Species') :: ['sheep'] @entity69516 :: ('105603615', 'Gene') :: ['TIMP-4'] @entity2914 :: ('MESH:D065630', 'Disease') :: ['congenital diaphragmatic hernia'] @entity69514 :: ('443115;100192422', 'Gene') :: ['MMP-2 and -9', 'matrix metalloproteinase (MMP)-2 and -9'] @entity432 :: ('MESH:D001157', 'Disease') :: ['fetoscopic balloon occlusion', 'Tracheal occlusion'] @entity31581 :: ('443115(Tax:9940)', 'Gene') :: ['MMP-2']
@entity742 :: (9940,Species) :: ['sheep']
