AIM: To study the purifying method and characteristics of new gosling @entity20332 (NGVEV),the etiological agent of new gosling @entity1800 (NGVE) which was first recognized in China, as well as the pathomorphological development in goslings infected artificially with NGVEV. METHODS: (1)NGVEV virions were purified by the procedure of treatment with @entity3636 and @entity1329 precipitation, dialysis to remove the @entity597 radical and @entity3104 ion and separation by gel filtration chromatography, and @entity214 -PAGE. (2)Forty-2-day-old White Sichuan goslings were orally administered with NGVEV and 24 hr later 2 birds were randomly selected and killed at 24hr intervals until @entity204 occurred. Specimens(duodenum, ileum, liver, heart, kidney, spleen, lung, proventriculus, pancreas, esophagus, and the intestinal embolus) were taken until all birds in this group died and were sectioned and stained with @entity1710 and @entity1711 and studied by light microscope. RESULTS: NGVEV shared the typical characteristics of @entity9504 and which structural proteins consisted of 15 polypeptides. @entity39 and sloughing of the epithelial cells covering the villus tips of the duodenum were first observed in goslings 2 days postinfection artificially with NGVEV. With the progress of @entity281 , this lesion rapidly occurred in the epithelium at the base of the villus and with infiltration of the inflammatory cells, the jejunum tended to be involved. With the intensification of mucosa @entity39 and inflammatory exudation of the small intestine, fibrinonecrotic @entity1681 was further developed and embolus composed of either intestinal contents wrapped by pseudo-membrane or of the mixture of fibrous exudate and @entity39 intestinal mucosa were observed in the middle-lower part of the small intestine. This structure occluded the intestinal tract and made the intestine dilated in appearance. The intestinal glandular cells underwent degeneration, @entity39 and might be found sloughed into the lumen. @entity548 and @entity450 could be observed on the lung and kidney. Epithelial cells of the renal tubular underwent degeneration. In some cases, @entity528 and @entity528 could be found in the liver and in some cases at a later stage of this disease the epithelial cells of @entity825 and proventriculus might be found sloughed. In some cases at an early stage of this disease, @entity299 and @entity548 could be observed. Esophagus, pancreas and brain were found normal. Analyses and comparisons between the pathologic lesions of NGVE and Gosling Plague (GP) were available in this paper as well. CONCLUSION: (1)NGVEV is adenovirus. (2)Pathological characteristic could be as the data for NGVE diagnosis.

Pathogenic and pathological characteristic of new XXXX first observed in China.

@entity825 :: ('MESH:D055090', 'Disease') :: ['trachea'] @entity9504 :: ('MESH:D000257', 'Disease') :: ['Adenovirus'] @entity548 :: ('MESH:D006470', 'Disease') :: ['hemorrhage', 'Hemorrhage'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis', 'necrotic', 'Necrosis'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity3636 :: ('MESH:D002725', 'Chemical') :: ['chloroform'] @entity450 :: ('MESH:D006940', 'Disease') :: ['hyperemia'] @entity299 :: ('MESH:D006331', 'Disease') :: ['cardiac hyperemia'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity1800 :: ('MESH:D014777', 'Disease') :: ['viral enteritis'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hemotoxylin'] @entity20332 :: ('MESH:D006525', 'Disease') :: ['viral enteritis virus'] @entity3104 :: ('MESH:D000644', 'Chemical') :: ['ammonium'] @entity1681 :: ('MESH:D004751', 'Disease') :: ['enteritis'] @entity1329 :: ('MESH:D000645', 'Chemical') :: ['ammonium sulfate'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death'] @entity528 :: ('MESH:D009410', 'Disease') :: ['granular degeneration', 'fatty degeneration'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS'] @entity597 :: ('CHEBI:16189', 'Chemical') :: ['sulfate']
@entity1800 :: (MESH:D014777,Disease) :: ['type gosling viral enteritis']
