Cells of the dendritic cell (DC) lineage, by their unique ability to stimulate naive T cells, may be of crucial importance in the development of protective immune responses to @entity3667 parasites. The aim of this study was to compare the impact of @entity3667 infection on DCs in BALB/c (susceptible, developing Th2 responses), C57BL/6 (resistant, developing @entity630 responses), and @entity5 @entity39 factor (TNF)(-/-) C57BL/6 @entity19 (susceptible, developing delayed and reduced @entity630 responses). We analyzed by immunohistochemistry the phenotype of infected cells in vivo. Granulocytes ( @entity17775 and macrophages ( @entity3804 (+)) appear as the mainly infected cells in primary lesions. In contrast, cells expressing @entity11805 , a DC specific marker, are the most frequently infected cells in draining lymph nodes of all @entity19 tested. These infected @entity11805 (+) cells harbored a particular morphology and cell surface phenotype in infected C57BL/6 and BALB/c @entity19 . @entity11805 (+) infected cells from @entity11897 @entity19 displayed a weak parasitic load and a dendritic morphology and frequently expressed @entity3804 or @entity11496 myeloid differentiation markers. In contrast, some @entity11805 (+) infected cells from BALB/c @entity19 were multinucleated giant cells. Giant cells presented a dramatic accumulation of parasites and differentiation markers were not detectable at their surface. In all @entity19 , lymph node @entity11805 (+) infected cells expressed a low major histocompatibility complex II level and no detectable @entity11787 expression. Our results suggest that infected @entity11805 (+) DC-like cells might constitute a reservoir of parasites in lymph nodes.

Amastigote load and cell surface phenotype of infected cells from lesions and lymph nodes of susceptible and resistant XXXX infected with @entity3667 .

@entity17775 :: ('546644(Tax:10090)', 'Gene') :: ['GR1(+))'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity11496 :: ('13733(Tax:10090)', 'Gene') :: ['F4/80'] @entity11787 :: ('12524(Tax:10090)', 'Gene') :: ['CD86'] @entity3667 :: ('5664', 'Species') :: ['L. major', 'Leishmania'] @entity11897 :: ('12274(Tax:10090)', 'Gene') :: ['C57BL/6 and TNF(-/-) C57BL/6'] @entity11805 :: ('16411(Tax:10090)', 'Gene') :: ['CD11c'] @entity3804 :: ('16409(Tax:10090)', 'Gene') :: ['CD11b'] @entity630 :: ('57314(Tax:10090)', 'Gene') :: ['Th1']
@entity19 :: (10090,Species) :: ['mice']
