UNASSIGNED: CK2 is a @entity563 / @entity1889 protein kinase, which is so important for many aspects of cellular regulation that life without CK2 is impossible. Here, we analysed CK2 during adipogenic differentiation of @entity1 mesenchymal stem cells (hMSCs). With progress of the differentiation CK2 protein level and the kinase activity decreased. Whereas CK2a remained in the nucleus during differentiation, the localization of @entity37822 showed a dynamic shuttling in the course of differentiation. Over the last years a large number of inhibitors of CK2 kinase activity were generated with the idea to use them in @entity5 therapy. Our results show that two highly specific inhibitors of CK2, @entity15440 and @entity69707 , reduced its kinase activity in proliferating hMSC with a similar efficiency. CK2 inhibition by @entity69707 resulted in nearly complete inhibition of differentiation whereas, in the presence of @entity15440 , differentiation proceeded similar to the controls. In this case, differentiation was accompanied by the loss of @entity15440 inhibitory function. By analysing the subcellular localization of @entity6864 , we found a shift from a nuclear localization at the beginning of differentiation to a more cytoplasmic localization in the presence of @entity69707 . Our data further show for the first time that a certain level of CK2 kinase activity is required for adipogenic stem cell differentiation and that inhibition of CK2 resulted in an altered localization of @entity6864 , an early regulator of differentiation.

Protein kinase CK2 is necessary for the adipogenic differentiation of XXXX mesenchymal stem cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity69707 :: ('MESH:C543110', 'Chemical') :: ['quinalizarin'] @entity37822 :: ('1460', 'Gene') :: ['CK2b'] @entity15440 :: ('MESH:C555142', 'Chemical') :: ['CX-4945'] @entity563 :: ('CHEBI:17822', 'Chemical') :: ['serine'] @entity1889 :: ('CHEBI:26986', 'Chemical') :: ['threonine'] @entity6864 :: ('5468', 'Gene') :: ['PPARy2']
@entity1 :: (9606,Species) :: ['human']
