Raffinose family oligosaccharides (RFOs) are synthesized by a set of galactosyltransferases, which sequentially add @entity3649 units from @entity41389 to @entity969 . The accumulation of RFOs was studied in maturing seeds of two @entity3587 ( @entity3587 ) lines with contrasting RFO composition. Seeds of the line SD1 accumulated @entity52825 as the predominant RFO, whereas @entity69559 , the next higher homolog of @entity52825 , was almost absent. In seeds of the line RRRbRb, a high level of @entity69559 was accumulated alongside with @entity52825 . The increase in @entity69559 in developing RRRbRb seeds was associated with @entity41389 -dependent @entity69559 synthase activity. In addition, a @entity41389 -independent enzyme activity was detected, which catalyzed transfer of a @entity3649 residue from one @entity52825 molecule to another. The two enzyme activities synthesizing @entity69559 showed an optimum at pH 7.0. Both activities were almost undetectable in SD1. Maximum activity of @entity52825 synthase was about 4-fold higher in RRRbRb compared with SD1, whereas the activities of @entity41389 synthase and @entity9592 synthase were only about 1.5-fold higher in RRRbRb. The levels of @entity41389 synthase and @entity52825 synthase activity were reflected by steady-state levels of corresponding mRNAs. We suggest that the accumulation of @entity69559 in RRRbRb was controlled by a coordinated up-regulation of the last steps of @entity69559 biosynthesis.

Analysis of the raffinose family oligosaccharide pathway in XXXX seeds with contrasting carbohydrate composition.

@entity69559 :: ('MESH:C570815', 'Chemical') :: ['verbascose'] @entity52825 :: ('MESH:C005695', 'Chemical') :: ['stachyose'] @entity41389 :: ('MESH:C013536', 'Chemical') :: ['galactinol'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity9592 :: ('MESH:D011887', 'Chemical') :: ['raffinose'] @entity3649 :: ('MESH:D005690', 'Chemical') :: ['galactose'] @entity3587 :: ('3888', 'Species') :: ['Pisum sativum', 'pea']
@entity3587 :: (3888,Species) :: ['pea']
