Intracellular @entity3689 transport across plant membranes is critical for metabolic pathways which are often split between different organelles. In addition, transport of @entity1522 across the plasma membrane enables the distribution of organic @entity1568 through the saps between leaves and developing organs. @entity3689 importers have been studied for more than two decades, and their role in this process is well-documented. While equally important, @entity3689 exporters are not well-characterized. The over-expression of @entity69579 , encoding a small membrane protein with one transmembrane domain, leads to enhancement of @entity3689 export by @entity958 cells, @entity3264 secretion at the leaf margin, early senescence and size reduction of the plant, possibly caused by the stimulation of @entity3689 exporter(s). Previous work reported the identification of suppressor mutations of the @entity69579 over-expression phenotype, which affected the @entity69579 and LOG2 genes, the latter encoding a membrane-bound ubiquitin ligase interacting with @entity69579 . The present study focuses on the characterization of three additional suppressor mutations affecting @entity69579 . Size, phenotype, @entity3264 transport and @entity3689 tolerance were recorded for recapitulation plants and over-expressors of mutagenized @entity69579 proteins. Unexpectedly, the over-expression of most mutated @entity69579 led to plants with enhanced @entity3689 export, but failing to display secretion of @entity3264 and size reduction. The results show that the various effects triggered by @entity69579 over-expression can be dissociated from one another by mutagenizing specific residues. The fact that these residues are not necessarily conserved suggests that the diverse biochemical properties of the @entity69579 protein are not only born by the characterized transmembrane and VIMAG domains. These data provide a better understanding of the structure/function relationships of @entity69579 and may enable modifying @entity3689 export in plants without detrimental effects on plant fitness.

Suppressor mutations in the @entity3264 Dumper1 protein dissociate disturbance in XXXX transport from other characteristics of the Gdu1D phenotype.

@entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity1568 :: ('MESH:D009584', 'Chemical') :: ['nitrogen'] @entity3264 :: ('D018698', 'Chemical') :: ['glutamine'] @entity958 :: ('3702', 'Species') :: ['Arabidopsis'] @entity69579 :: ('829301(Tax:3702)', 'Gene') :: ['GDU1'] @entity3689 :: ('D000596', 'Chemical') :: ['Amino acid', 'amino acid']
@entity3689 :: (D000596,Chemical) :: ['amino acid']
