An original method to induce heat stress was used to clarify the time course of changes in @entity1457 proteins (HSPs) in @entity35 skeletal muscles during recovery after a single bout of heat stress. One hindlimb was inserted into a stainless steel can and directly heated by raising the air temperature inside the can via a flexible heater twisted around the steel can. Muscle temperature was increased gradually and maintained at 42 degrees C for 60 min. Core rectal and contralateral muscle temperatures were increased <1.5 degrees C during the heat stress. @entity28379 , @entity34445 , and @entity1457 cognate (HSC) 73 content in the slow soleus and fast plantaris in both limbs were determined immediately (0 h) and 2, 4, 8, 12, 24, 36, 48, or 60 h after heat stress. Within 0-4 h, all HSPs were approximately 1.5- to 2.2-fold higher in heat-stressed than contralateral soleus. Compared with the contralateral plantaris, the heat-stressed plantaris had a higher (1.5-fold) @entity28379 content immediately and 2 h after heat stress and a higher (2.5- to 6.8-fold) @entity34445 content between 24 and 48 h after heat stress. Plantaris HSC73 content was not affected by heat stress. This unique heat-stress method provides advantages over existing systems; muscle temperature can be controlled precisely during heating and the HSP response can be compared between muscles in heat-stressed and contralateral limbs of individual @entity35 . Results show a differential response of HSPs in the soleus and plantaris during recovery after heat stress; soleus demonstrated a more rapid and broader HSP response to heat stress than plantaris.

Muscle type-specific response of @entity28379 , XXXX , and HSC73 during recovery after elevation of muscle temperature.

@entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity1457 :: ('MESH:D012769', 'Disease') :: ['heat shock'] @entity34445 :: ('24472(Tax:10116)', 'Gene') :: ['HSP72'] @entity28379 :: ('63868(Tax:10116)', 'Gene') :: ['HSP60']
@entity34445 :: (24472(Tax:10116),Gene) :: ['HSP72']
