1. Previous data demonstrate that the tricyclic antidepressant, @entity5829 , induces glucocorticoid receptor (GR) translocation from the cytoplasm to the nucleus in L929 cells and increases @entity619 -induced GR-mediated gene transcription in L929 cells stably transfected with the @entity19 mammary @entity5 virus- @entity2755 acetyltransferase ( @entity6584 -CAT) reporter gene (LMCAT cells) (Pariante et al., 1997). 2. To extend these findings, the present study has investigated the effects of 24 h coincubation of LMCAT cells with @entity619 and @entity3659 , @entity8991 , @entity903 , @entity837 or @entity905 . 3. All antidepressants, except @entity905 , enhanced GR-mediated gene transcription, with @entity8991 having the greatest effect (10 fold increase). Twenty-four hours coincubation of cells with @entity5829 , @entity8991 or @entity903 , also enhanced GR function in the presence of @entity701 , but not of @entity2819 . 4. It is proposed that these effects are due to the antidepressants inhibiting the L929 membrane @entity134 transporter, which actively extrudes @entity619 and @entity701 from the cell, but not @entity2819 . This is further confirmed by the fact that @entity8991 failed to enhance GR-mediated gene transcription in the presence of @entity619 when the membrane @entity134 transporter was blocked by @entity1855 . 5. The membrane @entity134 transporters that regulate access of glucocorticoids to the brain in vivo, like the multiple drug resistance p-glycoprotein, could be a fundamental target for antidepressant action.

Antidepressants enhance glucocorticoid receptor function in vitro by modulating the membrane XXXX transporters.

@entity6584 :: ('11757', 'Species') :: ['MMTV'] @entity8991 :: ('MESH:D002997', 'Chemical') :: ['clomipramine'] @entity905 :: ('MESH:D005473', 'Chemical') :: ['fluoxetine'] @entity3659 :: ('MESH:D000639', 'Chemical') :: ['amitriptyline'] @entity5829 :: ('MESH:D003891', 'Chemical') :: ['desipramine'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumour'] @entity1855 :: ('MESH:D014700', 'Chemical') :: ['verapamil'] @entity903 :: ('MESH:D017374', 'Chemical') :: ['paroxetine'] @entity2819 :: ('MESH:D003345', 'Chemical') :: ['corticosterone'] @entity619 :: ('MESH:D003907', 'Chemical') :: ['dexamethasone'] @entity837 :: ('MESH:D015283', 'Chemical') :: ['citalopram'] @entity134 :: ('CHEBI:35341', 'Chemical') :: ['steroid'] @entity701 :: ('MESH:D006854', 'Chemical') :: ['cortisol'] @entity2755 :: ('MESH:D002701', 'Chemical') :: ['chloramphenicol']
@entity134 :: (CHEBI:35341,Chemical) :: ['steroid']
