Pluripotent stem cells are increasingly used for therapeutic models, including transplantation of neural progenitors derived from @entity1 embryonic stem cells (hESC). Recently, long noncoding RNA (lncRNA), including @entity15047 ( @entity15047 ) derived from the @entity11591 - @entity41386 -imprinted locus, were found to be expressed during neural developmental events. Their deregulations are associated with various @entity16 . The @entity11591 - @entity41386 -imprinted locus encodes abundant noncoding RNA (ncRNA) that are regulated by differential methylation on the locus. The aim of our research was to study the correlation between the @entity11591 - @entity41386 -derived ncRNA and the capacity of hESC neural lineage differentiation. We classified hESC into @entity15047 -ON and @entity15047 -OFF based on the expression levels of @entity15047 as well as its downstream microRNA by qRT-PCR. Initial embryoid body (EB) formation was conducted to examine the 3 germ layer's differentiation ability. Complementary DNA microarray was used to analyse the gene expression profiles of hESC. Directed neural lineage differentiation was performed, followed by analysis of neural lineage marker expression levels and neurite formation via qRT-PCR and immunocytochemistry methods to investigate the capacity of neural differentiation in @entity15047 -ON and @entity15047 -OFF hESC. As for statistics, error bars indicate standard error of the mean. Student's t-test was used for calculating P-values, and a P-value of less than 0.05 was considered to be significant. Our results showed that @entity15047 -ON and @entity15047 -OFF hESC differed greatly in @entity11591 - @entity41386 -derived ncRNA expression levels, but had comparable pluripotency gene expression profiles. Genes related to nervous system development and @entity5 were differentially expressed in @entity15047 -OFF hESC, where @entity11591 - @entity41386 -derived ncRNA were repressed compared to @entity15047 -ON ones before differentiation. In neural lineage-like cells derived from @entity15047 -OFF hESC, lower expression levels of neural lineage markers and @entity73 were observed compared to @entity15047 -ON hESC at the same time points after differentiation. We suggest that the expression of @entity11591 - @entity41386 -derived lncRNA, @entity15047 , can be used as a simple and effective screening criterion for identifying @entity15047 -ON hESC with activation of @entity11591 - @entity41386 -imprinted ncRNA as starting materials to benefit neural lineage-associated studies.

346 expression of imprinted noncoding RNA from the XXXX - @entity41386 locus in @entity1 embryonic stem cells advantages neural lineage differentiation.

@entity1 :: ('9606', 'Species') :: ['human'] @entity11591 :: ('8788', 'Gene') :: ['DLK1'] @entity15047 :: ('55384', 'Gene') :: ['MEG3', 'Maternally Expressed Gene 3'] @entity5 :: ('MESH:D009369', 'Disease') :: ['neural cancers'] @entity16 :: ('MESH:D009422', 'Disease') :: ['neurological diseases'] @entity73 :: ('MESH:D003072', 'Disease') :: ['impaired neurite formation'] @entity41386 :: ('1735', 'Gene') :: ['DIO3']
@entity11591 :: (8788,Gene) :: ['dlk1']
