UNASSIGNED: @entity69077 (SYMMV, genus Carmovirus) was previously known to occur in South Korea and USA causing bright yellow mosaic in @entity1460 . In this study, SYMMV (Car-Mb14 isolate) was isolated from mungbean ( @entity2773 ) exhibiting mild mottling and puckering symptoms in the experimental field at Indian Agricultural Research Institute, New Delhi during 2012. The virus isolate, Car-Mb14 induced veinal mottling, mild mottling, chlorotic blotching, local and systemic @entity39 in @entity1460 , mungbean, blackgram, @entity11297 and guar bean, respectively. The symptomatology of the present isolate of SYMMV was different from the previously reported South Korean isolate, as the later did not induce symptoms in any of the above legumes other than @entity1460 . The present isolate was phylogenetically distinct and shared 90-93  % sequence identity in @entity40353 ( @entity40353 ) of 52 SYMMV isolates reported from Korea and USA. In order to know the serological relationships, the CP gene of the present isolate was over expressed as a 39  kDa protein in @entity526 and an antiserum of 1:16,000  titer against the recombinant CP was produced. Serological cross reactivity analysis revealed that SYMMV was serologically related to blackgram mottle virus but not to @entity67472 , the other legume infecting carmoviruses. The antiserum was used to detect prevalence of SYMMV in legume crops by ELISA. Out of 145 field samples of legumes (mungbean, blackgram, @entity11297 and @entity1460 ) collected from different places in India, SYMMV was detected only in 16 samples of mungbean and one sample of blackgram. The natural infection of SYMMV in mungbean and blackgram was further confirmed based on @entity40353 gene sequence. This study provides evidence of occurrence of a new variant of SYMMV with distinct symptom phenotype and extended host-range in India.

Diagnosis of a new variant of XXXX with extended host-range in India.

@entity11297 :: ('3885', 'Species') :: ['French bean'] @entity67472 :: ('12627', 'Species') :: ['cowpea mottle virus'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis'] @entity2773 :: ('157791', 'Species') :: ['Vigna radiata'] @entity1460 :: ('3847', 'Species') :: ['soybean'] @entity40353 :: ('64083', 'Gene') :: ['CP', 'coat protein'] @entity526 :: ('562', 'Species') :: ['E. coli'] @entity69077 :: ('578361', 'Species') :: ['Soybean yellow mottle mosaic virus']
@entity69077 :: (578361,Species) :: ['soybean yellow mottle mosaic virus']
