Dendritic cells (DC) are known to be the most potent @entity1412 and to stimulate antigen-specific T cell responses. Recently it was reported that @entity19 DC were also capable of modulating the innate immunity by stimulating NK cells through cell-to-cell contact. In the present study, we examined whether @entity1 DC could affect NK activity. Both monocyte-derived and @entity8730 (+) blood DC were tested. The addition of DC to cultures of @entity2940 (+) cells resulted in the significant dose-dependent enhancement of the killing activity against various NK-sensitive targets. The resultant activity was comparable to that induced by optimal concentrations of various cytokines, including @entity1199 , IL-12, @entity8252 and @entity379 . Interestingly, DC enhanced the @entity137 of CD3(-) @entity2940 (+) NK cells, but not that of CD3(+) @entity2940 (+) T cells. Experiments using transwells clearly demonstrated that the enhancement of NK activity by DC was mediated by soluble factors produced by DC. The culture supernatants of DC also stimulated NK activity. The treatment of both DC and their supernatants with anti-human IL-12 or @entity8923 antibodies did not block the enhancement of NK cell-mediated cytolysis by DC, indicating that other factor(s) produced by DC were responsible for the enhancement of NK activity. These results suggest that @entity1 myeloid DC can modulate innate immunity by enhancing NK activity.

@entity1 monocyte-derived and XXXX (+) blood dendritic cells enhance NK cell-mediated @entity137 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity2940 :: ('4684', 'Gene') :: ['CD56'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity8730 :: ('9308', 'Gene') :: ['CD83'] @entity8923 :: ('3606', 'Gene') :: ['IL-18'] @entity379 :: ('3458', 'Gene') :: ['IFN-gamma'] @entity1199 :: ('3558', 'Gene') :: ['IL-2'] @entity8252 :: ('3600', 'Gene') :: ['IL-15'] @entity1412 :: ('MESH:D011125', 'Disease') :: ['APC']
@entity8730 :: (9308,Gene) :: ['CD83']
