UNASSIGNED: Neutrophil extracellular trap (NET) formation is described as a tool of the innate host defence to fight against invading pathogens. Fibre-like DNA structures associated with proteins such as histones, cell-specific enzymes and antimicrobial peptides are released, thereby entrapping invading pathogens. It has been reported that several bacteria are able to degrade NETs by nucleases and thus evade the NET-mediated entrapment. Here we studied the ability of three different Yersinia serotypes to induce and degrade NETs. We found that the common @entity2820 serotypes O:3, O:8 and O:9 were able to induce NETs in @entity1 blood-derived neutrophils during the first hour of co-incubation. At later time points the NET amount was reduced, suggesting that degradation of NETs has occurred. This was confirmed by NET degradation assays with @entity10757 - @entity18257 - @entity598 -pre-stimulated neutrophils. In addition, we found that the Yersinia supernatants were able to degrade purified plasmid DNA. The absence of @entity141 and @entity1025 -ions, but not that of a protease inhibitor cocktail, completely abolished NET degradation. We therefore postulate that @entity2820 produces @entity141 / @entity1025 -dependent NET-degrading nucleases as shown for some Gram-positive pathogens.

XXXX -mediated degradation of neutrophil extracellular traps (NETs).

@entity1 :: ('9606', 'Species') :: ['human'] @entity1025 :: ('CHEBI:18420', 'Chemical') :: ['Mg(2+)'] @entity18257 :: ('MESH:D019814', 'Chemical') :: ['myristate'] @entity10757 :: ('MESH:C033085', 'Chemical') :: ['phorbol'] @entity598 :: ('CHEBI:47622', 'Chemical') :: ['acetate'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity2820 :: ('630', 'Species') :: ['Y. enterocolitica']
@entity2820 :: (630,Species) :: ['Yersinia enterocolitica']
