Most @entity1 cells have in their plasma membrane at least two types of lipid microdomains, non-invaginated lipid rafts and caveolae. @entity4495 (GPI)-anchored proteins constitute a class of proteins that are enriched in rafts but not caveolae at steady state. We have analyzed the effects of abolishing GPI biosynthesis on rafts, caveolae, and @entity165 levels. @entity46722 cells were obtained by screening for resistance to the pore-forming toxin aerolysin, which uses this class of proteins as receptors. Despite the absence of GPI-anchored proteins, mutant cells still contained lipid rafts, indicating that GPI-anchored proteins are not crucial structural elements of these domains. Interestingly, the caveolae-specific membrane proteins, @entity14582 , were up-regulated in @entity46722 cells, in contrast to @entity42537 and GM1, which were expressed at normal levels. Additionally, the number of surface caveolae was increased. This effect was specific since recovery of GPI biosynthesis by gene recomplementation restored caveolin expression and the number of surface caveolae to wild type levels. The inverse correlation between the expression of GPI-anchored proteins and @entity5689 was confirmed by the observation that overexpression of @entity5689 in wild type cells led to a decrease in the expression of GPI-anchored proteins. In cells lacking caveolae, the absence of GPI-anchored proteins caused an increase in @entity165 levels, suggesting a possible role of GPI-anchored proteins in @entity165 homeostasis, which in some cells, such as @entity3569 ovary cells, can be compensated by caveolin up-regulation.

Cross-talk between caveolae and XXXX -rich domains.

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity46722 :: ('MESH:C537277', 'Disease') :: ['GPI-deficient'] @entity5689 :: ('857', 'Gene') :: ['caveolin-1'] @entity42537 :: ('10211', 'Gene') :: ['flotillin-1'] @entity4495 :: ('MESH:D017261', 'Chemical') :: ['Glycosylphosphatidylinositol'] @entity3569 :: ('10029', 'Species') :: ['Chinese hamster'] @entity165 :: ('MESH:D002784', 'Chemical') :: ['cholesterol'] @entity14582 :: ('857;858', 'Gene') :: ['caveolin-1 and 2']
@entity4495 :: (MESH:D017261,Chemical) :: ['glycosylphosphatidylinositol']
