UNASSIGNED: Apoptosis is critical for the development of @entity2806 /reperfusion injury. @entity42459 (Trx-1) protein has been reported to have anti-apoptotic effects in a variety of cell types, and it has been implicated in @entity1545 after @entity2500 ( @entity2500 ). Thus, we studied the effects of Trx1 silencing after @entity2500 in @entity35 and examined whether inhibition of endogenous Trx1 could increase tissue levels of apoptosis. Male @entity35 (N=170) were subjected to 1h of @entity2500 followed by 24h of reperfusion. Trx1 siRNAs were injected into @entity35 brains 24h prior to @entity2500 . Then, 24h after @entity2500 , brains were collected from euthanized @entity35 for investigation. Treatment with Trx1 siRNA significantly increased mortality, @entity146 , and @entity385 volume and exacerbated neuronal cell apoptotic death after @entity2500 . Western blot revealed increased expression of apoptotic proteins such as P- @entity26898 , P-JNK, P- @entity12220 , cleaved caspase-3 and increased the level of cytochrome c in the cytosolic fraction in the Trx1 siRNA-treated group. Co-immunoprecipitation assay suggested an interaction between Trx1 and @entity26898 in normal @entity35 brains and Trx1 siRNA dissociated @entity26898 -Trx1 binding complex. Our data suggest that Trx1 siRNA increases apoptotic stress-induced @entity26898 activation and this represents further evidence that Trx1 is an endogenous anti-apoptotic molecule that diminishes focal @entity2806 /reperfusion injury. Its mechanism of action is likely related to attenuation of the @entity26898 -JNK/ @entity12220 signaling pathway.

Inhibition of @entity42459 with siRNA exacerbates apoptosis by activating the @entity26898 -JNK/ XXXX pathway in brain of a stroke model @entity35 .

@entity1545 :: ('MESH:D020214', 'Disease') :: ['brain injury'] @entity42459 :: ('116484(Tax:10116)', 'Gene') :: ['Thioredoxin-1'] @entity2806 :: ('MESH:D002545', 'Disease') :: ['cerebral ischemia'] @entity146 :: ('MESH:D001523', 'Disease') :: ['behavioral deficits'] @entity2500 :: ('MESH:D020244', 'Disease') :: ['MCAO', 'middle cerebral arterial occlusion', 'MCAO injury', 'middle cerebral artery occlusion'] @entity26898 :: ('365057(Tax:10116)', 'Gene') :: ['ASK1'] @entity35 :: ('10116', 'Species') :: ['rat', 'Sprague-Dawley rats', 'rats'] @entity12220 :: ('81649(Tax:10116)', 'Gene') :: ['p38'] @entity385 :: ('MESH:D002544', 'Disease') :: ['cerebral infarction']
@entity12220 :: (81649(Tax:10116),Gene) :: ['p38']
