Purpose: Laser photocoagulation of @entity989 ( @entity989 ) is used to stimulate the regenerative processes of the @entity989 . However, the molecular mechanisms that control @entity989 proliferation and the epithelial-mesenchymal transition (EMT) during regeneration remain poorly understood. We investigated the role of Wnt/b-catenin signaling in the regeneration of @entity19 @entity989 after laser photocoagulation. Methods: C57BL/6J @entity19 were photocoagulated unilaterally. To determine the b-catenin-dependent Wnt signal transduction in the photocoagulated @entity989 , the expression levels of Wnts, b-catenin, and their target genes were analyzed using real time-PCR and Western blotting. Retinal pigment epithelium proliferation and EMT were determined by @entity10080 ( @entity10081 ) incorporation assay and by profiling expression of EMT markers, respectively, in eyes injected intravitreally with a Wnt/b-catenin signaling antagonist, @entity26735 , after laser photocoagulation. Results: Expression of several of the 19 Wnt genes was significantly increased in laser-treated @entity989 . The expression levels of b-catenin signaling target genes @entity1896 , @entity5005 , and @entity22836 were higher in laser-treated @entity989 than in control @entity989 . The number of @entity10081 -positive cells in the laser-treated area was significantly lower in the @entity26735 -injected group than in the laser-treated group or laser-treated + vehicle-injected group. There were more @entity5005 - and @entity22836 -positive cells after laser photocoagulation and markedly fewer in the @entity26735 -injected group. @entity5005 - and @entity22836 -positive cells were colocalized with @entity10081 -positive cells. The EMT markers vimentin and @entity3113 ( @entity3113 ) were upregulated in the laser-treated area and significantly downregulated in the @entity26735 -injected group. Conclusions: Laser photocoagulation activates a Wnt/b-catenin signal transduction pathway, promoting both @entity989 proliferation and EMT. Wnt/b-catenin signaling also upregulates the expression of @entity5005 and @entity22836 , key transcription factors in @entity989 formation. Our results demonstrate an important role for Wnt/b-catenin signaling in @entity989 proliferation and EMT, and suggest that manipulating Wnt/b-catenin signaling may have therapeutic potential for @entity989 regeneration.

Wnt/b-Catenin Signaling Mediates Regeneration of Retinal Pigment Epithelium After Laser Photocoagulation in XXXX Eye.

@entity1896 :: ('12443(Tax:10090)', 'Gene') :: ['cyclin D1'] @entity3113 :: ('11475(Tax:10090)', 'Gene') :: ['a-smooth muscle actin', 'a-SMA'] @entity10081 :: ('MESH:C032766', 'Chemical') :: ['EdU'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity5005 :: ('18424(Tax:10090)', 'Gene') :: ['Otx2'] @entity10080 :: ('MESH:C031086', 'Chemical') :: ["5-ethynyl-2'-deoxyuridine"] @entity26735 :: ('13380(Tax:10090)', 'Gene') :: ['Dkk-1'] @entity989 :: ('MESH:D012164', 'Disease') :: ['retinal pigment epithelium', 'RPE'] @entity22836 :: ('17342(Tax:10090)', 'Gene') :: ['Mitf']
@entity19 :: (10090,Species) :: ['Mouse']
