We have shown that the 16D10 antigen located on the mucin-like @entity1473 -terminal domain of the feto-acinar pancreatic protein ( @entity36994 ) is expressed at the surface of @entity1 @entity788 cell lines such as SOJ-6 cell line. Furthermore, an in vivo study indicates that targeting this cell-membrane glycopeptide by the use of the monoclonal antibody (mAb) 16D10 inhibits the growth of SOJ-6 xenografts in @entity19 . To validate the potential use of the mAb16D10 in immune therapy, this study examined the expression of 16D10 antigens at the surface of @entity1 pancreatic @entity420 versus control tissues. We examined the reactivity of mAb16D10 and mAb8H8 with @entity1124 ( @entity1124 ) compared with controls by using immunohistochemistry and confocal laser scanning microscopy. mAb8H8 does react with control or nontumoral @entity1 pancreatic tissues. mAb16D10 has a strong and specific reactivity with @entity1124 and does not react with other @entity5 of epithelia or normal tissues tested. Notable, mAb16D10 mostly recognizes membrane of tumoral cells. Furthermore, mAb8H8 and mAb16D10 recognized a protein of 110 to 120 kDa in homogenates of nontumoral and tumoral @entity1 pancreatic tissues, respectively. This size correlates with that of @entity36994 or with that of the normal counterpart of @entity36994 , the so-called bile salt-dependent lipase. The results suggest that mAb16D10 presents a unique specificity against @entity1124 ; consequently, it could be effective in immune therapy of this @entity5 . Furthermore, mAb16D10 and mAb8H8 pair might be useful for diagnosis purpose in discriminating tumoral from nontumoral @entity1 pancreatic tissues.

Monoclonal antibody 16D10 to the XXXX -terminal domain of the feto-acinar pancreatic protein targets pancreatic neoplastic tissues.

@entity1 :: ('9606', 'Species') :: ['human'] @entity788 :: ('MESH:D010190', 'Disease') :: ['pancreatic tumor'] @entity1124 :: ('MESH:D021441', 'Disease') :: ['pancreatic ductal adenocarcinomas', 'PDAC'] @entity19 :: ('10090', 'Species') :: ['nude mice'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancers', 'cancer'] @entity36994 :: ('1056', 'Gene') :: ['FAPP'] @entity420 :: ('MESH:D000230', 'Disease') :: ['adenocarcinomas'] @entity1473 :: ('CHEBI:46883', 'Chemical') :: ['COOH']
@entity1473 :: (CHEBI:46883,Chemical) :: ['COOH']
