PURPOSE: More accurate assessment of prognosis is important to further improve the choice of risk-related therapy in @entity521 ( @entity521 ) @entity1 . In this study, we aimed to establish and validate a prognostic miRNA signature for @entity1 with @entity521 and tested it in both fresh frozen and archived @entity406 -fixed paraffin-embedded (FFPE) samples. EXPERIMENTAL DESIGN: Four hundred-thirty @entity1 mature miRNAs were profiled in two @entity1 subgroups with maximally divergent clinical courses. Univariate logistic regression analysis was used to select miRNAs correlating with @entity521 @entity1 survival. A 25-miRNA gene signature was built using 51 training samples, tested on 179 test samples, and validated on an independent set of 304 fresh frozen @entity5 samples and 75 archived FFPE samples. RESULTS: The 25-miRNA signature significantly discriminates the test @entity1 with respect to progression-free and overall survival (P < 0.0001), both in the overall population and in the cohort of high-risk @entity1 . Multivariate analysis indicates that the miRNA signature is an independent predictor of @entity1 survival after controlling for current risk factors. The results were confirmed in an external validation set. In contrast to a previously published mRNA classifier, the 25-miRNA signature was found to be predictive for @entity1 survival in a set of 75 FFPE @entity521 samples. CONCLUSIONS: In this study, we present the largest @entity521 miRNA expression study so far, including more than 500 @entity521 @entity1 . We established and validated a robust miRNA classifier, able to identify a cohort of high-risk @entity521 @entity1 at greater risk for adverse outcome using both fresh frozen and archived material.

miRNA expression profiling enables risk stratification in archived and fresh XXXX samples.

@entity1 :: ('9606', 'Species') :: ['children', 'human', 'patients', 'patient'] @entity406 :: ('MESH:D005557', 'Chemical') :: ['formalin'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity521 :: ('MESH:D009447', 'Disease') :: ['NB', 'neuroblastoma']
@entity5 :: (MESH:D009369,Disease) :: ['neuroblastoma tumor']
