BACKGROUND: Serum reticulin and endomysium autoantibodies are highly @entity1593 -specific, and the autoantigens have been shown to be derived from @entity1 fibroblasts. Among @entity1 tissues, the umbilical cord also expresses these antigens. This study was conducted to compare different autoantibody tests and especially to elucidate whether @entity1 umbilical cord is a suitable substrate in tests and whether the cord jelly-derived fibroblasts express the antigens. METHODS: The indirect immunofluorescence method was used to detect the tissue and Wharton's jelly-derived fibroblast antibodies in 334 @entity1593 and control sera samples. Affinity chromatography studies were used to show the correlation between @entity1 fibroblast-derived autoantigens and tissue and gliadin antibodies. The jelly-derived fibroblasts were used as antigen in a whole-cell enzyme-linked immunosorbent assay. RESULTS: @entity1593 @entity1 sera showed IgA-class @entity1 umbilical cord antibody with high sensitivity (100%) and specificity (99%). All @entity1593 @entity1 sera tested showed in indirect immunofluorescence the molecules expressed by Wharton's jelly-derived fibroblasts. The whole-cell fibroblast autoantibody enzyme-linked immunosorbent assay had a sensitivity of 100% and a specificity of 81%. @entity1 fibroblast-derived @entity1593 autoantigens absorbed most of the IgA responsible for @entity1 umbilical cord antibodies but not the IgA responsible for gliadin antibodies in the same sera. CONCLUSIONS: Wharton's jelly-derived fibroblast autoantibodies tested in a novel whole-cell enzyme-linked immunosorbent assay correlated well with the @entity1 umbilical cord but not with gliadin antibodies.

Autoantibodies in XXXX : importance of fibroblasts.

@entity1 :: ('9606', 'Species') :: ['human', 'patient', 'Human'] @entity1593 :: ('MESH:D002446', 'Disease') :: ['celiac disease', 'Celiac disease']
@entity1593 :: (MESH:D002446,Disease) :: ['celiac disease']
