1. The maltase, sucrase, isomaltase and palatinase activities of the @entity1573 small intestine are localized in particles that sediment when centrifuged at 100000g for 90min. 2. Solubilization of the particle-bound disaccharidases without loss of activity was achieved by digestion with papain. Trypsin was less effective and caused a preferential solubilization of the sucrase, isomaltase and palatinase activities. 3. On Sephadex G-200 columns, the solubilized preparations yielded two disaccharidase peaks. The first peak was eluted close to the void volume of the column and contained all the sucrase, isomaltase and palatinase activities and some of the maltase activity. The remainder of the maltase activity was eluted beyond the total volume of the column. 4. Precipitation with @entity1068 did not affect the behaviour of the disaccharidases of gel filtration. 5. The maltase activity of the second peak on rechromatography in a buffer containing 0.01m- @entity7969 was eluted close to the void volume. 6. Similar pH optima but different K(m) values were obtained for the maltase activities of the two peaks. 7. Heat-inactivation studies showed that the first peak contained two disaccharidase enzymes; one hydrolysed @entity969 and @entity7969 and the other hydrolysed @entity26791 , @entity70475 and @entity7969 . The second peak contained three disaccharidase enzymes all specific for the hydrolysis of @entity7969 . 8. It is proposed that the intestinal disaccharidases of the @entity1573 exist in the form of two complexes: a sucrase-isomaltase complex and a maltase complex.

Heat inactivation and sephadex chromatography of the small-intestine disaccharidases of the XXXX .

@entity70475 :: ('MESH:C008189', 'Chemical') :: ['palatinose'] @entity7969 :: ('MESH:D008320', 'Chemical') :: ['maltose'] @entity26791 :: ('MESH:D007534', 'Chemical') :: ['isomaltose'] @entity1573 :: ('9031', 'Species') :: ['chick'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity1068 :: ('MESH:D000431', 'Chemical') :: ['ethanol']
@entity1573 :: (9031,Species) :: ['chick']
