OBJECTIVE: Besides neurons, activated microglia and astrocytes in the spinal cord dorsal horn (SCDH) contribute to the pathogenesis of @entity1118 . Electroacupuncture (EA) has been used widely to treat various chronic @entity158 , however, the underlying mechanisms of EA are still not fully understood. METHODS: Male @entity35 were randomly divided into four groups, including an untreated healthy Control group (n=14), a @entity129 ( @entity129 ) group that underwent @entity129 and remained untreated (n=25), a True- @entity129 +EA group that underwent @entity129 followed by EA treatment (n=25), and a Sham- @entity129 group that underwent sham surgery and remained untreated (n=15). @entity129 was performed unilaterally at L5 and EA was applied to ST36 and BL60 bilaterally once per day. Paw withdrawal thresholds (PWTs) were measured ipsilaterally at baseline and 1, 3, 7, and 14   days after ligation. Activation of microglia and astrocytes in the SCDH were examined bilaterally by immunofluorescence staining, and concentrations of @entity3426 ( @entity3426 ) and interleukin ( @entity568 ) were measured in the ipsilateral SCDH by ELISA. RESULTS: @entity129 significantly decreased PWTs and activated glial cells in the superficial laminae of the ipsilateral SCDH. In @entity35 with @entity129 , @entity40 ( @entity40 ) immunoreactivity peaked at 7   days and was maintained until 14   days post-ligation, while anti-integrin alphaM (OX-42) immunoreactivity peaked at 3   days and declined gradually. EA significantly alleviated @entity129 -induced mechanical @entity1931 . Furthermore, EA reduced microglial activation (OX-42 positive ratios) in the lumbar SCDH at 3   days post-ligation and suppressed astrocyte activation ( @entity40 positive ratios) at all time points observed. CONCLUSIONS: EA stimulation alleviates @entity129 -induced @entity9 , at least in part through inhibition of spinal glial activation. Moreover, inhibition of spinal microglia and astrocyte activation may contribute to the immediate effects and maintenance of EA analgesia, respectively.

Inhibition of spinal microglia and astrocytes contributes to the anti-allodynic effect of electroacupuncture in XXXX induced by spinal nerve ligation.

@entity129 :: ('MESH:D003389', 'Disease') :: ['SNL', 'True-spinal nerve ligation'] @entity1931 :: ('MESH:D006930', 'Disease') :: ['allodynia'] @entity568 :: ('24498(Tax:10116)', 'Gene') :: ['IL-6'] @entity3426 :: ('24494(Tax:10116)', 'Gene') :: ['interleukin-1b', 'IL-1b'] @entity158 :: ('MESH:D010146', 'Disease') :: ['pain diseases'] @entity40 :: ('24387(Tax:10116)', 'Gene') :: ['GFAP', 'glial fibrillary acidic protein'] @entity35 :: ('10116', 'Species') :: ['Sprague-Dawley rats', 'rats'] @entity1118 :: ('MESH:D059350', 'Disease') :: ['chronic pain'] @entity9 :: ('MESH:D009437', 'Disease') :: ['neuropathic pain']
@entity9 :: (MESH:D009437,Disease) :: ['neuropathic pain']
