UNASSIGNED: Nucleosome organization exhibits dynamic properties depending on the cell state and environment. Histone proteins, fundamental components of nucleosomes, are subject to chemical modifications on particular residues. We examined the effect of substituting modifiable residues of four core histones with the non-modifiable residue @entity3929 on nucleosome dynamics. We mapped the genome-wide nucleosomes in 22 histone mutants of @entity375 and compared the nucleosome alterations relative to the wild-type strain. Our results indicated that different types of histone mutation resulted in different phenotypes and a distinct reorganization of nucleosomes. Nucleosome occupancy was altered at telomeres, but not at centromeres. The first nucleosomes upstream (-1) and downstream (+1) of the @entity1021 ( @entity1021 ) were more dynamic than other nucleosomes. Mutations in histones affected the nucleosome array downstream of the @entity1021 . Highly expressed genes, such as ribosome genes and genes involved in glycolysis, showed increased nucleosome occupancy in many types of histone mutant. In particular, the H3K56A mutant exhibited a high percentage of dynamic genomic regions, decreased nucleosome occupancy at telomeres, increased occupancy at the +1 and -1 nucleosomes, and a slow growth phenotype under stress conditions. Our findings provide insight into the influence of histone mutations on nucleosome dynamics.

Nucleosome alterations caused by mutations at modifiable histone residues in XXXX .

@entity375 :: ('4932', 'Species') :: ['Saccharomyces cerevisiae'] @entity3929 :: ('D000409', 'Chemical') :: ['alanine'] @entity1021 :: ('MESH:D020922', 'Disease') :: ['transcription start site', 'TSS']
@entity375 :: (4932,Species) :: ['Saccharomyces cerevisiae']
