It is well known that traumatic injuries of the CNS induce a gliotic reaction, characterized by the presence of reactive astrocytes. Reactive astrocytes exhibit enhanced expression of the astrocyte-specific intermediate filament, @entity40 ( @entity40 ), @entity1184 , and thickened processes. Recently, we have demonstrated that injuries of the CNS induce a re-expression of an embryonic intermediate filament-associated protein, IFAP-70/280 kDa. Based on IFAP-70/280 kDa immunolabeling, we have shown that reactive astrocytes, activated by @entity130 , can be divided into two major groups: 1. persistent IFAP+/ @entity40 + cells which are close to the wound in the area of @entity66 . transient IFAP-/ @entity40 + cells which are farther from the wound. In this study, we use @entity1603 incorporation to examine proliferation in these two groups of reactive astrocytes induced by stab injury of the @entity35 cerebrum. Triple/double-label immunofluorescence microscopy was performed using antibodies to IFAP-70/280 kDa, @entity40 , and @entity1603 . The results showed that @entity1603 + reactive astrocytes ( @entity40 +) were always IFAB-70/280 kDa+ as well. However, not all IFAP+ reactive astrocytes are @entity1603 +. @entity1603 + signal was not observed in any IFAP- reactive astrocytes. At five days post-lesion, IFAP+ reactive astrocytes were increasing in the area of the wound (0-50 micrograms from the wound edge), but had reached a peak in the proximal area (50-800 micrograms away from the wound edge). At eight days post-lesion, IFAP+ reactive astrocytes achieved the highest percentage in the wound area. At the same time, @entity1603 -containing reactive astrocytes occupied an area closer to the wound. By 20 days post-lesion, following the formation of the gliotic scar at the stab-wound, a few IFAP+/ @entity40 + cells still persisted. @entity1603 -containing reactive astrocytes were only observed in the scar. These results indicate that many IFAP+ reactive astrocytes close to the wound, in contrast to the IFAP- ones farther from the wound, appear to regain their proliferative potential to increase in number and participate in the formation of the gliotic scar.

Proliferation of a subpopulation of reactive astrocytes following needle-insertion lesion in XXXX .

@entity1603 :: ('MESH:D001973', 'Chemical') :: ['BrdU'] @entity66 :: ('MESH:D004194', 'Disease') :: ['glial scar, and 2'] @entity1184 :: ('MESH:D006984', 'Disease') :: ['hypertrophy'] @entity40 :: ('24387(Tax:10116)', 'Gene') :: ['GFAP', 'glial fibrillary acidic protein'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity130 :: ('MESH:D014947', 'Disease') :: ['stab-wound injury']
@entity35 :: (10116,Species) :: ['rat']
