HYPOTHESIS: @entity619 ( @entity3784 ) protects against radiation-induced loss of auditory hair cells (HCs) in @entity35 organ of Corti (OC) explants by reducing levels of oxidative stress and apoptosis. BACKGROUND: Radiation-induced @entity1274 ( @entity393 ) is progressive, dose-dependent, and irreversible. Currently, there are no preventative therapeutic modalities for radiation-induced @entity393 . @entity3784 is a synthetic @entity479 that can potentially target many of the pathways involved in radiation-induced @entity2167 . METHODS: Whole OC explants were dissected from 3-day-old @entity35 cochleae exposed to specific dosages of single-fraction radiation (0, 2, 5, 10, or 20Gy), were either untreated or treated with @entity3784 (75, 150, 300 g/mL), and then cultured for 48 or 96hours. Confocal microscopy for oxidative stress (CellRox, 48h) and apoptosis (TUNEL assay, 96h) and fluorescent microscopy for viable HC counts ( @entity2822 - @entity6286 , 96h) were performed. Analysis of variance and Tukey post hoc testing were used for statistical analysis. RESULTS: Radiation exposure initiated dose-dependent losses of inner and outer HCs, predominantly in the basal turns of the OC explants. @entity3784 protected against radiation-induced HC losses in a dose-dependent manner. @entity3784 significantly reduced levels of oxidative stress and apoptosis in radiation-injured OC explants (p<0.001). CONCLUSIONS: Radiation-initiated HC losses were dose-dependent in OC explants. @entity3784 treatment protected explant HCs against radiation-initiated losses by decreasing the levels of oxidative stress and apoptosis. @entity3784 may potentially be a therapeutic modality for preventing radiation-induced @entity393 ; further in vivo studies are necessary.

XXXX Protects Against Radiation-induced Loss of Auditory Hair Cells In Vitro.

@entity3784 :: ('CHEBI:4470', 'Chemical') :: ['DXM'] @entity2167 :: ('MESH:D006311', 'Disease') :: ['ototoxicity'] @entity2822 :: ('CHEBI:37926', 'Chemical') :: ['fluorescein isothiocyanate'] @entity6286 :: ('MESH:D010590', 'Chemical') :: ['phalloidin'] @entity619 :: ('MESH:D003907', 'Chemical') :: ['Dexamethasone'] @entity1274 :: ('MESH:D006319', 'Disease') :: ['sensorineural hearing loss'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity393 :: ('MESH:D034381', 'Disease') :: ['HL'] @entity479 :: ('D013256', 'Chemical') :: ['steroid']
@entity619 :: (MESH:D003907,Chemical) :: ['Dexamethasone']
