UNASSIGNED: Complete crush or cut severance of sciatic nerve axons in @entity35 and other mammals produces immediate loss of axonal continuity. @entity717 subserved by those axons is restored only after months, if ever, by outgrowths regenerating at    1   mm/day from the proximal stumps of severed @entity1347 . The distal stump of a severed axon typically begins to degenerate in 1-3 days. We recently developed a @entity968 ( @entity970 ) fusion technology, consisting of sequential exposure of severed axonal ends to @entity2421 @entity141 -free saline, @entity6693 , @entity970 in distilled water, and finally @entity141 -containing isotonic saline. This study examines factors that affect the @entity970 fusion restoration of axonal continuity within minutes, as measured by conduction of action potentials and diffusion of an intracellular fluorescent dye across the lesion site of @entity35 sciatic nerves completely cut or crush severed in the midthigh. Also examined are factors that affect the longer-term @entity970 fusion restoration of lost behavioral functions within days to weeks, as measured by the sciatic functional index. We report that exposure of cut-severed axonal ends to @entity141 -containing saline prior to @entity970 fusion and stretch/tension of proximal or distal @entity1347 of cut-severed axons decrease @entity970 fusion success. Conversely, trimming cut-severed ends in @entity141 -free saline just prior to @entity970 fusion increases @entity970 fusion success. @entity970 fusion prevents or retards the @entity6441 of cut-severed axons, as assessed by measures of axon diameter and G ratio. @entity970 fusion may produce a paradigm shift in the treatment of @entity1897 .    2016 Wiley Periodicals, Inc.

Effects of extracellular calcium and surgical techniques on restoration of axonal continuity by XXXX fusion following complete cut or crush severance of @entity35 sciatic nerves.

@entity1347 :: ('MESH:C537775', 'Disease') :: ['axonal segments'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity6693 :: ('MESH:D008751', 'Chemical') :: ['methylene blue'] @entity1897 :: ('MESH:D010523', 'Disease') :: ['peripheral nerve injuries'] @entity2421 :: ('MESH:D009123', 'Disease') :: ['hypotonic'] @entity6441 :: ('MESH:D014855', 'Disease') :: ['Wallerian degeneration'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity717 :: ('MESH:D009069', 'Disease') :: ['Loss of locomotor functions'] @entity970 :: ('CHEBI:46793', 'Chemical') :: ['PEG'] @entity968 :: ('MESH:D011092', 'Chemical') :: ['polyethylene glycol']
@entity968 :: (MESH:D011092,Chemical) :: ['polyethylene glycol']
