OBJECTIVE: To simulate the chemical microenvironment of injured brain tissue, and to explore the effect of this chemical microenvironment on temperature sensitive umbilical cord mesenchymal stem cells (tsUC). METHODS: @entity35 models of @entity131 ( @entity131 ) were made by fluid percussion injury, and then the brain tissue extracts of the injured regions were acquired. @entity1 umbilical cord mesenchymal stem cells (UC) were isolated and cultured, and the tsUC were obtained through the infection of temperature-sensitive Simian 40 Large T- antigen (ts-SV40LT) retrovirus. After that, both the two kinds of cells were cultured on the @entity2975 gels which mimicking the elastic modulus of brain. Four groups were included: UC cultured under normal temperature (UC group), UC cultured added brain tissue extract under normal temperature (UC plus extract group), tsUC cultured under mild @entity2332 (tsUC group), and tsUC added brain tissue extract under mild @entity2332 for 3 days, then normal temperature for 4 days (tsUC plus extract group). After 24 hours, the apoptosis level was checked. Cell growth and morphological changes in each group were given dynamic observation. Seven days later, cell immunofluorescences were implemented for examining neural differentiation level. RESULTS: Compared with UC plus extract group, the apoptosis and proliferation in UC plus extract group were significantly reduced (P < 0.01) and increased (P < 0.01) respectively. Cell immunofluorescence showed that the both @entity538 and Neuron positive cells were significantly enhanced in UC plus extract group than those in tsUC plus extract group. CONCLUSION: tsUC combining with mild @entity2332 could significantly reverse injury induced cell apoptosis, improve cell proliferation and neural differentiation under chemical microenvironment after @entity1545 , which confirmed the adaptation and resistance of tsUC under mild @entity2332 after @entity131 .

[Effect of chemical microenvironment after XXXX on temperature-sensitive umbilical cord mesenchymal stem cells].

@entity1 :: ('9606', 'Species') :: ['Human'] @entity1545 :: ('MESH:D020214', 'Disease') :: ['brain injury'] @entity131 :: ('MESH:D001930', 'Disease') :: ['TBI', 'traumatic brain injury'] @entity538 :: ('2670', 'Gene') :: ['GFAP'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity35 :: ('10116', 'Species') :: ['Rat'] @entity2332 :: ('MESH:D007035', 'Disease') :: ['hypothermia']
@entity131 :: (MESH:D001930,Disease) :: ['traumatic brain injury']
