Prostanoid generation may proceed via both isoforms of cyclooxygenase, @entity9774 and @entity4665 . @entity9774 is thought to be ubiquitously expressed, whereas @entity4665 is mostly assumed to be dynamically regulated, responding to inflammatory stimuli. The cellular localization of @entity9774 and @entity4665 in the lung, an organ with high cyclooxygenase activity, is not known. In normal @entity35 lungs the expression and localization of @entity9774 and @entity4665 were examined with immunogold- @entity575 staining and the RT-PCR technique. Quantitative image analysis of the staining intensity was performed by measuring mean gray values of digitized epipolarization images. Expression of both @entity9774 and @entity4665 was readily detectable in @entity35 lungs. @entity9774 immunoreactivity localized predominantly to bronchial epithelial cells, smooth muscle cells of large hilum veins, and (with lower expression) to alveolar macrophages and pulmonary artery endothelial cells. The most intense @entity4665 staining was noted in macrophage- and mast cell-like cells, detected in close vicinity to the bronchial epithelium and in the connective tissue surrounding the vessels. In addition, strong @entity4665 expression was found in smooth muscle cells of partially muscular vessels and large veins of the hilum. Bronchial epithelial cells displayed @entity4665 immunoreactivity with limited intensity. Alveolar macrophages and alveolar septal cells were only occasionally stained with anti- @entity4665 antibodies. Both @entity9774 and @entity4665 are constitutively expressed in several cell types of normal @entity35 lung, but display clearly different patterns of cellular localization. @entity4665 may not be related only to @entity55 , but is suggested to be implicated in regulatory processes under physiological conditions as well.

Cyclooxygenase isoenzyme localization and mRNA expression in XXXX lungs.

@entity55 :: ('MESH:D011014', 'Disease') :: ['lung inflammation'] @entity575 :: ('MESH:D012834', 'Chemical') :: ['silver'] @entity9774 :: ('26195(Tax:10116)', 'Gene') :: ['Cox-1'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity4665 :: ('26198(Tax:10116)', 'Gene') :: ['Cox-2']
@entity35 :: (10116,Species) :: ['rat']
