BACKGROUND: Current anti- @entity5 drug therapy results in systemic side effects due to non-specific uptake by normal healthy noncancerous tissues. To alleviate this difficulty, many attempts have been devoted to the development of new delivery systems such as polymeric Nanoparticles (NPs). In this study, we prepared ICD-85 NPs based on @entity14917 and analyzed the cytotoxic activity of ICD-85 NPs relative to free ICD-85 on primary lamb kidney cells. METHODS: ICD-85 loaded @entity14917 nanoparticles were prepared by ionic gelation method and were characterized by the particle size, size distribution and Fourier Transform Infrared (FT-IR) spectroscopy. The in vitro @entity137 was evaluated by @entity98 assay and membrane integrity was evaluated by measuring Lactate Dehydrogenase (LDH) activity. The morphological alterations of untreated and treated cells were assessed by light inverted microscope. RESULTS: @entity98 assay showed that ICD-85 NPs could significantly decrease the in vitro @entity137 on primary lamb kidney cells compared to the free ICD-85. The IC10 value at 72 hours was increased from 9  2.7  g/ml for free ICD-85 to 52  4.3  g/ml for ICD-85 NPs. LDH assay demonstrated that free ICD-85 had dose-dependent @entity137 on primary lamb kidney cells while ICD-85 NPs exhibited significantly decreased @entity137 at equivalent concentrations. Moreover, morphological analysis showed no significant difference between control and treated cells with ICD-85 NPs. CONCLUSION: Based on the results obtained in the present study it can be concluded that encapsulation of ICD-85 with @entity14917 nanoparticles can reduce its necrotic effect on primary lamb kidney cells.

Comparative Cytotoxic Evaluation of Free and XXXX Nanoparticle-Encapsulated ICD-85 on Primary Lamb Kidney Cells.

@entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity14917 :: ('MESH:C008970', 'Chemical') :: ['sodium alginate'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity']
@entity14917 :: (MESH:C008970,Chemical) :: ['Sodium Alginate']
