OBJECTIVE: To investigate the effect of @entity1605 (II) with medicinal guide Radix Achyranthis Bidentatae (Ach) on bone marrow stem cells (BMSCs) homing to @entity39 area after @entity2530 of the femoral head (ONFH) frozen by liquid @entity1568 in @entity95 as well as to explore the mechanism of prevention and treatment for ONFH. METHODS: The animal model of ONFH was established by liquid @entity1568 frozen on the @entity95 left hind leg. Forty-eight @entity95 were randomly assigned to sham-operated group, model group, Huogu II group, and Huogu II plus Ach group, with 12 @entity95 in each. During the course of ONFH animal model establishment, all @entity95 were subcutaneously injected with recombinant @entity1 @entity1261 [rhG-CSF, 30  g/(kg  day) for continuous 7 days]. Meanwhile, normal saline and decoction of the two formulae were administrated by gavage, respectively. White blood cells (WBC) were counted in peripheral blood before and after injection of rhG-CSF. Materials were drawn on the 2nd and 4th weeks after model built; bone @entity1160 protein (BGP) and @entity9040 ( @entity9040 ) levels in serum were tested. Histopathologic changes were observed by @entity1710 and @entity1711 (HE) staining. @entity9040 mRNA levels were detected with in situ hybridization (ISH) staining. @entity1603 ( @entity1603 ) and stromal cell derived factor 1 (SDF-1) were measured by immunohistochemical assay in femoral head of the left hind leg. RESULTS: Compared with the shamoperated group, the ratio of empty lacuna, serum BGP, and SDF-1 level in the model group increased significantly, and @entity9040 in both serum and femoral head decreased significantly. However, in comparison with the model group, the empty lacuna ratio of Huogu II group and Huogu II plus Ach group decreased obviously in addition to the levels of serum BGP and @entity9040 , and the expressions of @entity9040 mRNA, @entity1603 , and SDF-1 increased significantly. Above changes were particularly obvious in Huogu II plus Ach group. BGP and SDF-1 on the 2nd week and empty lacuna rate and serum @entity9040 level on the 4th week in Huogu II group significantly exceeded their counterparts. On the 2nd week, only in Huogu II plus Ach group that the @entity1603 counting rose significantly. On the 4th week, empty lacuna rate and serum @entity9040 level in Huogu II plus Ach group exceeded those in Huogu II group distinctively. CONCLUSIONS: To a certain extent, the medicinal guide Ach improves the preventive and therapeutic effects of @entity1605 on experimental ONFH model. The possible mechanism of this is related to its promoting effect on directional homing of BMSCs to the @entity39 area.

Effect of @entity1605 (II) with medicinal guide Radix Achyranthis Bidentatae on bone marrow stem cells directional homing to @entity39 area after @entity2530 of the femoral head in XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity1603 :: ('MESH:D001973', 'Chemical') :: ['BrdU', "5-Bromo-2'-deoxyuridine"] @entity1605 :: ('OMIM:217000', 'Disease') :: ['Huogu II Formula'] @entity1568 :: ('MESH:D009584', 'Chemical') :: ['nitrogen'] @entity1261 :: ('1440', 'Gene') :: ['granulocyte colony-stimulating factor'] @entity1160 :: ('CHEBI:28300', 'Chemical') :: ['glutamine'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity95 :: ('9986', 'Species') :: ['rabbits', 'rabbit', 'Japanese White rabbits'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hematoxylin'] @entity9040 :: ('100009349(Tax:9986)', 'Gene') :: ['BMP2', 'bone morphogenetic protein 2'] @entity2530 :: ('MESH:D010020', 'Disease') :: ['osteonecrosis']
@entity95 :: (9986,Species) :: ['rabbit']
