UNASSIGNED: Growth arrested 3T3 cells have been used as feeder cells in @entity1 epidermal keratinocyte cultures to produce cultured epidermal autografts for the treatment of burns. The feeder cells were ideally growth-arrested by gamma-irradiation. Alternatively, @entity851 by @entity1138 treatment is a cost effective option. We compared the functional efficacy of these two approaches in keratinocyte cultures by colony forming efficiency, the net growth area of colonies, @entity1603 labeling and histological features of cultured epidermal sheets. The growth area estimation involved a semi-automated digital technique using the Adobe Photoshop and comprised of isolation and enumeration of red pixels in @entity21237 -stained keratinocyte colonies. A further refinement of the technique led to the identification of critical steps to increasing the degree of accuracy and enabling its application as an extension of colony formation assay. The results on feeder cell functionality revealed that the gamma irradiated feeders influenced significantly higher colony forming efficiency and larger growth area than the @entity1138 treated feeders. The @entity1603 labeling study indicated significant stimulation of the overall keratinocyte proliferation by the gamma irradiated feeders. The cultured epidermal sheets produced by gamma feeders were relatively thicker than those produced by @entity1138 feeders. We discussed the clinical utility of @entity1138 feeders from the viewpoint of cost-effective burn care in developing countries.

An evaluation of the choice of feeder cell XXXX for the production of cultured epidermis.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1603 :: ('MESH:D001973', 'Chemical') :: ['BrdU'] @entity851 :: ('MESH:D006130', 'Disease') :: ['growth arrest'] @entity21237 :: ('CHEBI:52334', 'Chemical') :: ['Rhodamine B'] @entity1138 :: ('MESH:D016685', 'Chemical') :: ['mitomycin C']
@entity851 :: (MESH:D006130,Disease) :: ['growth arrest']
