Two hybrid genes ( @entity40110 -HuIFN-gamma2 and @entity40110 -HuIFN-gamma3) were constructed on the basis of @entity742 @entity40110 ( @entity40110 ) and @entity1 @entity379 (HuIFN-gamma) gene sequences. They were used to direct HuIFN-gamma synthesis in the mammary gland of @entity19 . HuIFN-gamma was efficiently produced in the mammary gland of @entity19 . @entity40110 -HuIFN-gamma2 transgenic females expressed HuIFN-gamma in the milk at concentrations up to 570 mg/ml, and @entity40110 -HuIFN-gamma3 transgenic females expressed up to 350 mg/ml. All females carrying the @entity40110 -HuIFN-gamma3 gene expressed HuIFN-gamma in their milk. No significant changes were observed in the HuIFN-gamma expression level during the lactation period. Using RT-PCR analysis, ectopic expression for both hybrid genes was found in @entity19 . Despite ectopic expression of HuIFN-gamma in @entity19 , their development and pregnancy were normal. The heritability of the HuIFN-gamma expression level in milk was demonstrated up to the F2 generation. This work demonstrates that hybrid genes have the potential to develop in transgenic domestic animals producing HuIFN-gamma in milk.

Efficient XXXX @entity379 expression in the mammary gland of @entity19 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity742 :: ('9940', 'Species') :: ['sheep'] @entity19 :: ('10090', 'Species') :: ['transgenic mice'] @entity379 :: ('3458', 'Gene') :: ['interferon-gamma'] @entity40110 :: ('443385(Tax:9940)', 'Gene') :: ['beta-lactoglobulin', 'BLG']
@entity1 :: (9606,Species) :: ['human']
