UNASSIGNED: @entity1 -induced pluripotent stem cells (hiPSCs)-based cell therapy holds promise for treating stress @entity322 (SUI). However, safety concerns, especially tumorgenic potential of residual undifferentiated cells in hiPSC derivatives, are major barriers for its clinical translation. An efficient, fast and clinical-scale strategy for purifying committed cells is also required. Our previous studies demonstrated the regenerative effects of hiPSC-derived smooth muscle progenitor cells (pSMCs) on the injured urethral sphincter in SUI, but the differentiation protocol required fluorescence-activated cell sorting (FACS) which is not practical for autologous clinical applications. In this study, we examined the efficacy and safety of hiPSC-derived pSMC populations sorted by FDA-approved magnetic-activated cell sorting (MACS) using cell-surface marker @entity2280 for restoring urethral sphincter function. Although the heterogeneity of MACS-sorted pSMCs was higher than that of FACS-sorted pSMCs, the percentage of undifferentiated cells dramatically decreased after directed differentiation in vitro. In vivo studies demonstrated long-term cell integration and no @entity5 formation of MACS-sorted pSMCs after transplantation. Furthermore, transplantation of MACS-sorted pSMCs into immunodeficient SUI @entity35 was comparable to transplantation with FACS-sorted pSMCs for restoration of the extracellular matrix metabolism and function of the urethral sphincter. In summary, purification of hiPSC derivatives using MACS sorting for @entity2280 expression represent an efficient approach for production of clinical-scale pSMCs for autologous stem cell therapy for regeneration of smooth muscle tissues.    Stem Cells Translational Medicine 2017.

Efficacy and Safety of Immuno-Magnetically Sorted Smooth Muscle Progenitor Cells Derived from XXXX -Induced Pluripotent Stem Cells for Restoring Urethral Sphincter Function.

@entity1 :: ('9606', 'Species') :: ['Human'] @entity322 :: ('MESH:D014549', 'Disease') :: ['urinary incontinence'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity2280 :: ('947', 'Gene') :: ['CD34']
@entity1 :: (9606,Species) :: ['Human']
