UNASSIGNED: @entity327 type b (Hib) causes acute @entity4008 (ABM) in @entity1 , with a mortality rate of about 3-6  % of the affected @entity1 . ABM can lead to death during a period of hours to several days and, hence, rapid and early detection of the infection is crucial. Aptamers, the short single-stranded DNA or RNA with high affinity to target molecules, are selected by a high-flux screening technique known as in vitro screening and systematic evolution of ligands by exponential enrichment technology (SELEX). In this study, whole-cell SELEX was applied for the selection of target-specific aptamers with high affinity to Hib. ssDNA aptamers prepared by lambda exonuclease were incubated with the target cells (Hib). The aptameric binding rate to Hib was characterized for binding affinity after seven SELEX rounds by flow cytometry. The aptamers with higher binding affinity were cloned. Four of 68 aptamer clones were selected for sequencing. The dissociation constant (Kd) of the high-affinity aptamer clones 45 and 63 were 47.10 and 28.46  pM, respectively. These aptamers did not bind to other bacterial species, including the seven @entity693 -causing bacteria. They showed distinct affinity to various @entity327 strains only. These aptamers showed the highest affinity to Hib and the lowest affinity to @entity327 type c and to other @entity693 -causing bacteria. Clone 63 could detect Hib in @entity1 ' cerebrospinal fluid (CSF) samples at 60  colony-forming units (CFU)/mL. The results indicate applicability of the aptamers for rapid and early detection of infections brought about by Hib.

DNA aptamers for the detection of XXXX type b by cell SELEX.

@entity1 :: ('9606', 'Species') :: ['children', 'patients'] @entity327 :: ('727', 'Species') :: ['H. influenzae', 'Haemophilus influenzae'] @entity4008 :: ('MESH:D016920', 'Disease') :: ['bacterial meningitis'] @entity693 :: ('MESH:D008581', 'Disease') :: ['meningitis']
@entity327 :: (727,Species) :: ['Haemophilus influenzae']
