@entity1 amnion mesenchymal stem cells (HAMSCs) can be obtained from @entity1 amniotic membrane, a highly abundant and readily available tissue. HAMSC sources present fewer ethical issues, have low immunogenicity, anti-inflammatory properties, considerable advantageous characteristics, and are considered an attractive potential treatment material in the field of regenerative medicine. We used a co-culture system to determine whether HAMSCs could promote osteogenesis in @entity1 bone marrow mesenchymal stem cells (HBMSCs). We isolated HAMSCs from discarded amnion samples and collected them using pancreatin/collagenase digestion. We cultured HAMSCs and HBMSCSs in basal medium. Activity of @entity623 ( @entity623 ), an early osteogenesis marker, was increased in the co-culture system compared to the control single cultures, which we also confirmed by @entity623 staining. We used immunofluorescence testing to investigate the effects of co-culturing with HAMSCs on HBMSC proliferation, which revealed that the co-culturing enhanced EdU expression in HBMSCs. Western blotting and quantitative real-time PCR indicated that co-culturing promoted osteogenesis in HBMSCs. Furthermore, @entity622 S staining revealed that extracellular matrix @entity460 levels in mineralized nodule formation produced by the co-cultures were higher than that in the controls. Using the same co-culture system, we further observed the effects of HAMSCs on osteogenic differentiation in primary osteoblasts by Western blotting, which better addressed the mechanism for HAMSCs in bone regeneration. The results showed HAMSCs are osteogenic and not only play a role in promoting HBMSC proliferation and osteogenic differentiation but also in osteoblasts, laying the foundation for new regenerative medicine methods.

XXXX amnion mesenchymal stem cells promote proliferation and osteogenic differentiation in @entity1 bone marrow mesenchymal stem cells.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity623 :: ('250', 'Gene') :: ['ALP', 'alkaline phosphatase'] @entity622 :: ('MESH:C010078', 'Chemical') :: ['Alizarin red'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium']
@entity1 :: (9606,Species) :: ['Human']
