Changes in membrane morphology and membrane protein dynamics based on its fluidity are critical for @entity5 @entity3 . However, this subject has remained unclear, because the spatial precision of previous in vivo imaging has been limited to the micrometer level and single molecule imaging is impossible. Here, we have imaged the membrane dynamics of @entity5 cells in @entity19 with a spatial precision of 7-9 nm under a confocal microscope. A @entity3 -promoting factor on the cell membrane, @entity14604 ( @entity14604 ), was labeled with quantum dots conjugated with an anti- @entity14604 antibody. Movements of @entity5 cells and @entity14604 during @entity3 were clearly observed in vivo. Images used to assess @entity14604 dynamics were taken of representative cells for four stages of @entity3 ; i.e. @entity5 cells far from blood vessels in @entity5 , near the vessel, in the bloodstream, and adherent to the inner vascular surface in the normal tissues near @entity5 were photographed. The diffusion constant of @entity14604 in static cells far from @entity5 blood vessels was smaller than in moving cells near the vessels and in the bloodstream. The diffusion constant of cells adhering to the inner vascular surface in the normal tissues was also very small. Cells formed membrane protrusion during migration. The @entity14604 diffusion constant on these pseudopodia was greater than in other membrane regions in the same cell. Thus, the dynamics of @entity14604 movement showed that membrane fluidity increases during intravasation, reaches a peak in the vessel, decreases during extravasation, and is also higher at locally formed pseudopodia.

In vivo nano-imaging of membrane dynamics in XXXX cells using quantum dots.

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity14604 :: ('14062(Tax:10090)', 'Gene') :: ['PAR1', 'protease-activated receptor 1'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis']
@entity3 :: (MESH:D009362,Disease) :: ['metastatic tumor']
