Insulin causes a suppression of whole-cell voltage-dependent outward current in cultured neurons from the @entity35 olfactory bulb. This suppression is time-dependent; it is mimicked by application of @entity19 @entity669 kinase inside the cell via the whole-cell patch electrode or by treatment of the olfactory bulb neurons with the @entity669 phosphatase inhibitor @entity7983 . The C-type inactivation properties of the outward current in olfactory bulb neurons resemble those of the cloned @entity12309 @entity972 channel. In addition, at picomolar concentrations at which it is specific for @entity12309 , the scorpion toxin margatoxin blocks most of the olfactory bulb neuron outward current. Immunocytochemical analysis demonstrates that @entity12309 is prominent in the cultured olfactory bulb neurons. To identify specific @entity535 residues that might be important for @entity972 current modulation, we examined the effects of @entity7983 and insulin on wild-type and mutant @entity12309 channels expressed in @entity1 embryonic kidney (HEK 293) cells. As shown previously, treatment with either @entity7983 or insulin suppresses @entity12309 current in these cells. Mutational analysis demonstrates that at least two distinct @entity669 residues are required for current suppression by @entity7983 . Insulin treatment stimulates the @entity669 phosphorylation of @entity12309 in HEK 293 cells, and a different combination of @entity669 residues is required for the current suppression by insulin. The results suggest that complex patterns of phosphorylation may be involved in the modulation of neuronal @entity972 current by receptor and nonreceptor @entity669 kinases.

Modulation of olfactory bulb neuron XXXX current by @entity669 phosphorylation.

@entity1 :: ('9606', 'Species') :: ['human'] @entity19 :: ('10090', 'Species') :: ['Src'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity7983 :: ('MESH:C076715', 'Chemical') :: ['pervanadate'] @entity972 :: ('MESH:D011188', 'Chemical') :: ['potassium'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity12309 :: ('3738', 'Gene') :: ['Kv1.3'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine']
@entity972 :: (MESH:D011188,Chemical) :: ['potassium']
