It was suggested in a previous study that cells of @entity69427 VE-C3 adhere to diesel fuel by synthesizing a capsular polysaccharide containing @entity413 and/or @entity3913 . To study the fine structure of cells and localization of bacterial polysaccharide in the presence of diesel fuel, two lectins were used: ConA, an agglutinin from Canavalia ensiformis specific for @entity3913 and/or @entity413 residues, and PNA, an agglutinin from Arachis hypogaea, for terminal @entity3649 residues. The lectins were conjugated with electron dense ferritin for transmission electron microscopy (TEM) and with @entity2822 ( @entity2823 ) for scanning confocal laser microscopy (SCLM). Samples were prepared by freeze substitution, which allows glycosylation to be determined in situ in thin sections of specimens. The distribution of glycosylation was imaged with and without treatment of specimens with their specific hapten ( @entity413 and @entity3649 ). The glycosylation activity produced a polysaccharide capsule. Emulsified diesel fuel nanodroplets were observed at the cell envelope perimeter. Fine structure of vesicles consisted of polysaccharide and diesel fuel nanodroplets. Lectin blotting analysis showed ConA-positive glycoprotein with an apparent molecular mass of 22 kDa in the outer membrane. Its production was induced by diesel fuel. This glycoprotein was probably responsible for bioemulsifying activity at the cell envelope. Several other glycoproteins were positive for PNA lectin, the main constituent migrating with an apparent molecular weight of 17.8 kDa. However, they were all constitutive and probably involved in cell biofilm formation at the oil surface.

Envelope glycosylation determined by lectins in microscopy sections of XXXX induced by diesel fuel.

@entity69427 :: ('52133', 'Species') :: ['Acinetobacter venetianus'] @entity2822 :: ('CHEBI:37926', 'Chemical') :: ['fluorescein isothiocyanate'] @entity3913 :: ('MESH:D008358', 'Chemical') :: ['mannose'] @entity2823 :: ('MESH:D016650', 'Chemical') :: ['FITC'] @entity3649 :: ('MESH:D005690', 'Chemical') :: ['galactose'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity69427 :: (52133,Species) :: ['Acinetobacter venetianus']
