INTRODUCTION: Cigarette smoking is a well-known risk factor for developing @entity65 , but the underlying mechanisms are largely unknown. Recent data suggest that vasocontractile receptor modulation could be an important factor. @entity24526 ( @entity24526 ) is important in the particle clearance in the lungs and knock-out (KO) @entity19 for this protein develop @entity2415 . @entity24526 is also weakly expressed in the vasculature. We aimed to investigate whether @entity24526 was important in the cardiovascular response to @entity1981 ( @entity1981 ), by utilizing @entity24526 KO @entity19 and a myograph setup. METHODS: @entity164 ( @entity164 ) and @entity24526 KO @entity19 were exposed to cigarette smoke (CS) or room air for 12 weeks. The pulmonary artery, left anterior descending coronary artery, and basilar artery (BA) were isolated and mounted in wire myographs. Contractile concentration response curves to @entity15486 and @entity858 were obtained. RESULTS: @entity1981 caused a leftward shift in the concentration response curves for @entity15486 in the BA for both @entity164 and @entity24526 KO. @entity858 , acting on the purinergic P2Y6 receptor, caused reduced contraction in the left descending artery and increased contraction in the BA in the @entity1981 @entity164 @entity19 . @entity24526 KO @entity19 displayed no smoke induced changes, but were surprisingly similar to the @entity1981 @entity164 . CONCLUSION: The contractility to @entity858 was altered in the brain and heart vasculature of @entity1981 @entity19 . @entity24526 KO (both control and @entity1981 ) and @entity1981 @entity164 had similar changes in contractility compared to control @entity164 . IMPLICATIONS: These results show that sub-chronic smoking induces vascular changes in the @entity164 , mainly for the purinergic P2Y6 receptor together with minor changes for the @entity15486 receptor. @entity24526 KO (both control and @entity1981 ) does not show any further changes compared to @entity1981 @entity164 .

Contractile Changes in the Vasculature After Subchronic Smoking: A Comparison Between Wild Type and @entity24526 Knock-Out XXXX .

@entity19 :: ('10090', 'Species') :: ['mice'] @entity858 :: ('MESH:D014530', 'Chemical') :: ['UDP'] @entity2415 :: ('MESH:D004646', 'Disease') :: ['emphysema'] @entity164 :: ('MESH:D017827', 'Disease') :: ['WT', 'Wild type'] @entity65 :: ('MESH:D002318', 'Disease') :: ['cardiovascular diseases'] @entity24526 :: ('20390(Tax:10090)', 'Gene') :: ['Surfactant protein D', 'SP-D'] @entity1981 :: ('OMIM:188890', 'Disease') :: ['CSE', 'cigarette smoke exposure'] @entity15486 :: ('13614(Tax:10090)', 'Gene') :: ['endothelin-1']
@entity19 :: (10090,Species) :: ['Mice']
