BACKGROUND AND OBJECTIVE: Low-level laser therapy (LLLT) or photobiomodulation therapy is emerging as a promising new therapeutic option for @entity82 in different damaged and/or diseased organs. However, the anti-fibrotic potential of this treatment needs to be elucidated and the cellular and molecular targets of the laser clarified. Here, we investigated the effects of a low intensity 635        5   nm diode laser irradiation on fibroblast-myofibroblast transition, a key event in the onset of @entity82 , and elucidated some of the underlying molecular mechanisms. MATERIALS AND METHODS: NIH/3T3 fibroblasts were cultured in a low serum medium in the presence of @entity2533 and irradiated with a 635        5   nm diode laser (continuous wave, 89   mW, 0.3   J/cm(2) ). Fibroblast-myofibroblast differentiation was assayed by morphological, biochemical, and electrophysiological approaches. Expression of @entity1476 and @entity1495 and of Tissue inhibitor of MMPs, namely @entity2843 and @entity1552 , after laser exposure was also evaluated by confocal immunofluorescence analyses. Moreover, the effect of the diode laser on transient receptor potential canonical channel (TRPC) 1/stretch-activated channel (SAC) expression and activity and on @entity2533 / @entity7379 signaling was investigated. RESULTS: Diode laser treatment inhibited @entity2533 -induced fibroblast-myofibroblast transition as judged by reduction of stress fibers formation, a-smooth muscle actin (sma) and type-1 collagen expression and by changes in electrophysiological properties such as resting membrane potential, cell capacitance and inwardly rectifying @entity3529 currents. In addition, the irradiation up-regulated the expression of @entity1476 and @entity1495 and downregulated that of @entity2843 and @entity1552 in @entity2533 -treated cells. This laser effect was shown to involve @entity7012 /SAC channel functionality. Finally, diode laser stimulation and @entity7012 functionality negatively affected fibroblast-myofibroblast transition by interfering with @entity2533 signaling, namely reducing the expression of @entity7379 , the @entity2533 downstream signaling molecule. CONCLUSION: Low intensity irradiation with 635        5   nm diode laser inhibited @entity2533 / @entity7379 -mediated fibroblast-myofibroblast transition and this effect involved the modulation of @entity7012 ion channels. These data contribute to support the potential anti-fibrotic effect of LLLT and may offer further informations for considering this therapy as a promising therapeutic tool for the treatment of tissue @entity82 . Lasers Surg. Med.    2015 Wiley Periodicals, Inc.

Low intensity 635   nm diode laser irradiation inhibits fibroblast-myofibroblast transition reducing @entity7012 channel expression/activity: New perspectives for tissue XXXX treatment.

@entity1552 :: ('7077', 'Gene') :: ['TIMP-2'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis'] @entity3529 :: ('D011188', 'Chemical') :: ['K(+)'] @entity7379 :: ('4088', 'Gene') :: ['Smad3'] @entity1476 :: ('4313', 'Gene') :: ['matrix metalloproteinase (MMP)-2', 'MMP-2'] @entity7012 :: ('7220', 'Gene') :: ['TRPC1'] @entity2843 :: ('7076', 'Gene') :: ['TIMP-1'] @entity2533 :: ('7040', 'Gene') :: ['TGF-b1', 'transforming growth factor (TGF)-b1'] @entity1495 :: ('4318', 'Gene') :: ['MMP-9']
@entity82 :: (MESH:D005355,Disease) :: ['fibrosis']
