OBJECTIVE: To test whether @entity1760 inhibits apoptosis during @entity7853 ( @entity7853 ) in the developing brain, we studied the effect of long-term @entity1760 treatment on an early marker of @entity528 (loss of @entity19744 ; @entity19744 ) and a marker of apoptosis (activated @entity4124 ) after @entity7853 in newborn @entity35 . METHODS: Seven-day-old @entity35 pups (n = 107) were exposed to unilateral carotid artery ligation and 2 hours of @entity519 (8% @entity26 in 92% @entity1568 ). @entity1760 was administered by a micro-osmotic pump implanted in the back, at an infusion rate of 75 mg/kg per hour for 3 days. @entity528 in the cerebral cortex and hippocampus was evaluated by loss of @entity19744 at 24 and 48 hours after @entity7853 and visually ranked by a semiquantitative, three-point scale (mild, moderate, and severe). @entity4124 activation was evaluated similarly in an adjacent section. Area and severity of the damaged lesion were compared between the two staining methods and between @entity1760 and controls, by chi(2) test and Fisher test. RESULTS: Three-day @entity1760 administration reduced loss of @entity19744 (from 33 of 50 to 21 of 45, P =.06) and activation of @entity4124 (from 34 of 50 to 21 of 45, P =.04) in the cerebral cortex after @entity7853 . There was no significant change in these immunostainings in the hippocampal regions. In the hippocampal @entity1251 , the severity ranked by activated @entity4124 staining was significantly less than that of @entity19744 staining. CONCLUSION: Long-term, post- @entity7853 treatment with @entity1760 inhibited @entity4124 activation and @entity19744 immunostaining, suggesting that @entity1760 inhibited apoptotic neuronal death of @entity7853 in 7-day-old @entity35 .

Effect of long-term, postasphyxial administration of magnesium sulfate on immunostaining of @entity19744 and activated XXXX in 7-day-old @entity35 brain.

@entity1568 :: ('MESH:D009584', 'Chemical') :: ['nitrogen'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity7853 :: ('MESH:D020925', 'Disease') :: ['HI', 'hypoxia-ischemia'] @entity519 :: ('MESH:D000860', 'Disease') :: ['hypoxia'] @entity1251 :: ('54232(Tax:10116)', 'Gene') :: ['CA3'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity4124 :: ('25402(Tax:10116)', 'Gene') :: ['Caspase-3', 'caspase-3'] @entity19744 :: ('25595(Tax:10116)', 'Gene') :: ['microtubule-associated protein-2', 'MAP-2'] @entity528 :: ('MESH:D009410', 'Disease') :: ['neuronal damage', 'Neuronal loss'] @entity1760 :: ('MESH:D008274', 'Chemical') :: ['magnesium', 'Magnesium']
@entity4124 :: (25402(Tax:10116),Gene) :: ['caspase-3']
