UNASSIGNED: Hepatic cell culture on a three-dimensional (3D) matrix or as a hepatosphere appears to be a promising in vitro biomimetic system for liver tissue engineering applications. In this study, we have combined the concept of a 3D scaffold and a spheroid culture to develop an in vitro model to engineer liver tissue for drug screening. We have evaluated the potential of @entity968 -alginate-gelatin (PAG) cryogel matrix for in vitro culture of @entity1 liver cell lines. The synthesized cryogel matrix has a flow rate of 7 mL/min and water uptake capacity of 94% that enables easy nutrient transportation in the in vitro cell culture. Young's modulus of 2.4 kPa and viscoelastic property determine the soft and elastic nature of synthesized cryogel. Biocompatibility of PAG cryogel was evaluated through @entity98 assay of HepG2 and Huh-7 cells on matrices. The proliferation and functionality of the liver cells were enhanced by culturing hepatic cells as spheroids (hepatospheres) on the PAG cryogel using temperature-reversible soluble-insoluble polymer, @entity1684 ( @entity1684 ). Pore size of the cryogel above 100  m modulated spheroid size that can prevent @entity519 condition within the spheroid culture. Both the hepatic cells have shown a significant difference (P < 0.05) in terms of cell number and functionality when cultured with @entity1684 . After 10 days of culture using 0.05% @entity1684 , the cell number increased by 11- and 7-fold in case of HepG2 and Huh-7 cells, respectively. Similarly, after 10 days of hepatic spheroids culture on PAG cryogel, the albumin production, @entity1133 secretion, and CYP450 activity were significantly higher in case of culture with @entity1684 . The developed tissue mass on the PAG cryogel in the presence of @entity1684 possess polarity, which was confirmed using F-actin staining and by presence of intercellular bile canalicular lumen. The developed cryogel matrix supports liver cells proliferation and functionality and therefore can be used for in vitro and in vivo drug testing.

Combined Effect of Cryogel Matrix and Temperature-Reversible Soluble-Insoluble Polymer for the Development of in Vitro XXXX Liver Tissue.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1133 :: ('MESH:D014508', 'Chemical') :: ['urea'] @entity1684 :: ('CHEBI:53234', 'Chemical') :: ['poly(N-isopropylacrylamide)', 'PNIPAAm'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity519 :: ('MESH:D000860', 'Disease') :: ['hypoxia'] @entity968 :: ('MESH:D011092', 'Chemical') :: ['poly(ethylene glycol)']
@entity1 :: (9606,Species) :: ['Human']
