Preeclampsia is characterized by dysfunctional endothelium and @entity73 . Recent studies suggest that the neuronal guidance SLIT/ROBO system regulates @entity5 angiogenesis. This study investigated if SLIT and ROBO are differentially expressed in healthy term and preeclamptic placentas and if @entity519 regulates SLIT and ROBO expression in placental trophoblast and endothelial cells. Total RNA and protein were extracted from placental tissues of healthy term (n = 5) and preeclamptic (n = 6) pregnancies and used for SLIT/ROBO expression analyses with reverse transcription-polymerase chain reaction (RT-PCR), real-time quantitative-PCR, and immunoblotting. @entity982 -embedded tissues were processed to localize SLIT/ROBO proteins in placental villi by immunohistochemistry. @entity1466 cells and @entity1 umbilical vein endothelial cells (HUVEC) were treated with 2% or 10% @entity26 or the @entity519 mimetic @entity5532 (100  M) to test if @entity519 regulates SLIT/ROBO expression. @entity49792 , @entity68640 , @entity25996 , and @entity68641 mRNA and proteins were detected in the placenta. @entity49792 and @entity25996 proteins localized in the syncytiotrophoblast, and @entity68640 , @entity25996 , and @entity68641 in capillary endothelium of the placental villi. Levels of @entity25996 and @entity68641 as well as sFLT1 (soluble fms-like @entity669 kinase-1) proteins were significantly greater in preeclamptic placentas compared to normal controls. @entity519 significantly increased both mRNA and protein levels of @entity49792 in BeWo cells and of @entity68640 , @entity25996 , and ROBB4 in HUVEC. Thus, trophoblast and endothelial coexpression of SLIT/ROBO suggests an autocrine/paracrine regulatory system for regulating placental function. Differential expression of SLITs and ROBOs in healthy term and preeclamptic placentas and @entity519 regulation of their expressions in placental cells implicate a potential pathophysiological role for this system in @entity1468 .

@entity1 placental expression of SLIT/ROBO signaling cues: effects of @entity1468 and XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity5532 :: ('MESH:D003676', 'Chemical') :: ['deferoxamine mesylate'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity73 :: ('MESH:D003072', 'Disease') :: ['impaired angiogenesis'] @entity1466 :: ('MESH:D002822', 'Disease') :: ['BeWo choriocarcinoma'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity49792 :: ('9353', 'Gene') :: ['SLIT2'] @entity519 :: ('MESH:D000860', 'Disease') :: ['Hypoxia', 'hypoxia'] @entity982 :: ('MESH:D010232', 'Chemical') :: ['Paraffin'] @entity25996 :: ('6091', 'Gene') :: ['ROBO1'] @entity1468 :: ('MESH:D011225', 'Disease') :: ['preeclampsia'] @entity68640 :: ('6586', 'Gene') :: ['SLIT3'] @entity68641 :: ('54538', 'Gene') :: ['ROBO4'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine']
@entity519 :: (MESH:D000860,Disease) :: ['hypoxia']
