UNASSIGNED: Detection and analysis of a small subpopulation of cells such as stem cells or @entity5 stem cells are recognized to be a key technique in a recent regeneration and @entity5 science. However, in the thyroid, no marker that identifies stem cells has been established yet. We previously established a novel method to analyze cells collected by fluorescence-activated cell sorting (FACS), named mRNA quantification after FACS (FACS-mQ). By using this method, the biological characteristics of the sorted cells can be determined by analyzing their gene expression profile. In this study, we analyzed the expression of stemness genes in a @entity35 thyroid cell lines FRTL5 using FACS-mQ. 3 stemness genes, @entity38572 , @entity24073 and @entity28430 , were expressed in @entity35 . In @entity35 cells, varied expression of @entity62835 ( @entity62835 ) among cells was observed by flow cytometry. Cell populations with high or low @entity62835 expression were analyzed by FACS-mQ. The cell population with low @entity62835 expression showed increased expression of the stemness genes. Furthermore, Ki67-positive cells showed increased expression of @entity62835 , which suggested that cells with high @entity62835 proliferated rapidly. These results indicated that @entity35 contains a cell population with high stemness gene expression and less differentiated features, resembling stem cells. These cells might regulate proliferation in FRTL5.

Expression Analysis of Stemness Genes in a XXXX Thyroid Cell Line FRTL5.

@entity28430 :: ('54254(Tax:10116)', 'Gene') :: ['GATA4'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity24073 :: ('312382(Tax:10116)', 'Gene') :: ['ABCG2'] @entity62835 :: ('24826(Tax:10116)', 'Gene') :: ['TG', 'thyroglobulin'] @entity38572 :: ('414065(Tax:10116)', 'Gene') :: ['NANOG'] @entity35 :: ('10116', 'Species') :: ['rat', 'FRTL5']
@entity35 :: (10116,Species) :: ['Rat']
