An experiment was conducted to determine whether pregnancy rates following the transfer of in vitro-produced embryos to heat-stressed @entity291 could be improved by 1) culturing embryos in the presence of @entity21659 ) treating recipients with @entity1176 . Lactating Holstein @entity291 (n = 260) were synchronized using a timed ovulation protocol. Embryos were produced in vitro and cultured with or without 100 ng/mL of @entity4208 . On d 7 after anticipated ovulation (d 0), a single embryo was transferred to all recipients with a palpable corpus luteum (n = 210). A subset of recipients (n = 164) was injected with either @entity1176 or placebo on d 11. Plasma @entity1420 concentrations on d 0 and 7 were used to determine the synchrony of recipients. Pregnancy was diagnosed at d 53 and 81 by rectal palpation. Among all recipients, transfer of @entity4208 -treated embryos increased pregnancy rate at d 53 (P < 0.05) and tended to increase pregnancy rate at d 81 (P < 0.06). Calving rate also tended to be higher for recipients that received @entity4208 -treated embryos (P < 0.07). Among the subset of synchronized recipients (n = 190), pregnancy rate at d 53 and d 81 and calving rate were higher (P < 0.05) for @entity4208 -treated embryos. The @entity1176 tended to increase pregnancy rate at d 53 for all recipients (P < 0.08) and the subset of synchronized recipients (P < 0.10). There were no effects of @entity1176 (P > 0.10) for pregnancy rate at d 81 and calving rate. The overall proportion of male @entity291 was 64.3%. There was no effect (P > 0.10) of embryo treatment or @entity1176 on the birth weight or sex ratio of @entity291 . Results of this experiment indicate that treatment of embryos with @entity4208 can improve pregnancy and calving rates following transfer of in vitro-produced embryos. Further research is necessary to determine whether the treatment of recipients with @entity1176 is a practical approach to increase pregnancy rates following in vitro embryo transfer.

Use of insulin-like growth factor-I during embryo culture and treatment of recipients with gonadotropin-releasing hormone to increase pregnancy rates following the transfer of in vitro-produced embryos to heat-stressed, lactating XXXX .

@entity1420 :: ('MESH:D011374', 'Chemical') :: ['progesterone'] @entity4208 :: ('104978413', 'Gene') :: ['IGF-I'] @entity1176 :: ('MESH:D007987', 'Chemical') :: ['GnRH'] @entity21659 :: ('104978413;281240', 'Gene') :: ['IGF-I and 2'] @entity291 :: ('9913', 'Species') :: ['cows', 'calves']
@entity291 :: (9913,Species) :: ['cows']
