OBJECTIVE: @entity130 of the lacrimal duct epithelium (LDE) can lead to a variety of lacrimal diseases. The purpose of this study was to characterize potential candidate cells for constructing a tissue-engineered LDE. METHODS: Different areas of the conjunctiva and lacrimal duct tissue were removed from male adult New Zealand white @entity95 for histological evaluation. @entity1710 and @entity1711 staining and immunohistochemical staining of @entity69673 , cytokeratin 4, Ki-67, and MUC5AC were observed by light microscopy. The surface morphologies of different epithelial tissues and cellular structures were examined using field-emission scanning electron microscopy and transmission electron microscopy. Epithelial cells were isolated from tissues and identified by specific markers. In vitro, proliferative ability and Western blot analyses of the @entity12914 ( @entity12914 ) of different epithelial cells cultured in identical environments were investigated and compared. RESULTS: Histologically, the epithelial specific markers, cytokeratin @entity69673 and cytokeratin 4, were expressed in the conjunctiva epithelium and the LDE. Notably, highly proliferative cells stained with Ki-67 were concentrated under the epithelium in a dome structure of the posterior palpebral conjunctiva. Differentiated goblet cells were also found to a lesser extent in this region. Primary palpebral and fornical conjunctival epithelial cells (PFCECs), bulbar conjunctival epithelial cells (BCECs), and lacrimal duct epithelial cells (LDECs) were successfully separated from tissues. In vitro, @entity95 PFCECs and LDECs grew faster and expressed more @entity12914 than BCECs. CONCLUSIONS: PFCECs are anatomically similar to LDECs. They also have similar morphological characteristics, immune phenotypes, and proliferation features. PFCECs are therefore potential candidate cells to replace LDECs in tissue engineering to treat lacrimal duct diseases.

Potential candidate cells for constructing tissue-engineered lacrimal duct epithelium: a histological and cytological study in XXXX .

@entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity95 :: ('9986', 'Species') :: ['rabbits', 'rabbit'] @entity69673 :: ('100008675(Tax:9986)', 'Gene') :: ['AE1+AE3', 'cytokeratin AE1+AE3'] @entity12914 :: ('100339381(Tax:9986)', 'Gene') :: ['PCNA', 'proliferating cell nuclear antigen'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['Hematoxylin'] @entity130 :: ('MESH:D014947', 'Disease') :: ['Injury and deficiency']
@entity95 :: (9986,Species) :: ['rabbits']
