UNASSIGNED: The primary issue undertaken in this study was to test the hypothesis that preadipocytes would have intrinsically elevated propensity to differentiate into mature adipocytes due to @entity281 . To prove that, the metabolic and molecular mechanisms responsible for HAdV31-induced adipogenesis were examined. 3T3L1 cells ( @entity19 embryonic fibroblast, adipose like cell line) were used as a surrogate model to analyze an increased proliferation, differentiation and maturation of preadipocytes infected with @entity1 adenovirus. An expression of E4orf1, @entity9322 , @entity5601 -y, @entity7501 , @entity5548 , @entity1243 and @entity844 synthase genes, intracellular lipid accumulation as well as cytokine release from the fat cells were assessed. Data showed that HAdV31 increased an expression of @entity9322 and @entity5601 -y genes leading to an enhanced differentiation of preadipocytes into fat cells. Besides, overexpression of @entity7501 and @entity844 synthase, and decreased expression of leptin caused an increased accumulation of intracellular lipids. Secretion of @entity310 and @entity650 from HAdV31-infected cells was strongly decreased, leading to unlimited virus replication. The results obtained from this study provided the evidences that HAdV31, likewise previously documented HAdV36, is a subsequent @entity1 adenovirus affecting the differentiation and lipid accumulation of 3T3L1 cells. This article is protected by copyright. All rights reserved.

An infection of XXXX adenovirus 31 affects the differentiation of preadipocytes into fat cells, its metabolic profile and fat accumulation.

@entity1 :: ('9606', 'Species') :: ['human'] @entity650 :: ('3569', 'Gene') :: ['IL-6'] @entity844 :: ('CHEBI:35366', 'Chemical') :: ['fatty acid'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity7501 :: ('2597', 'Gene') :: ['GAPDH'] @entity5548 :: ('2167', 'Gene') :: ['aP2'] @entity1243 :: ('3952', 'Gene') :: ['LEP'] @entity310 :: ('7124', 'Gene') :: ['TNF-a'] @entity281 :: ('MESH:D007239', 'Disease') :: ['HAdV31 infection'] @entity5601 :: ('5465', 'Gene') :: ['PPAR'] @entity9322 :: ('1051', 'Gene') :: ['C/EBP-b']
@entity1 :: (9606,Species) :: ['human']
