OBJECTIVE: To explore the effects of @entity4965 ( @entity4966 ) on cardiac developmental differentiation in @entity1 embryonic stem cells. METHODS: In this study, based on the @entity1 embryonic stem cells in vitro cardiac differentiation assay, we investigated the potential effect of @entity4966 exposure on the @entity1370 in embryo development. @entity1 embryonic stem cells were treated with @entity4966 at different concentrations of 100 ppb, 1 ppm, and 10 ppm and @entity3733 ( @entity3733 ) treated as control. The @entity98 assay was performed to examine the cytoplasmic toxicity of @entity4966 exposure. The beating percentages were recorded and the expression of cardiac specific gene was evaluated by PCR or flow cytometry. Also, real time PCR was performed to verify the micro array analysis on the expression level changes of genes which were involved in the @entity141 signal pathways. RESULTS: Compared with the control group, there was no significant difference in cell viability when cells were treated with @entity4966 at the concentrations of 100 ppb, 1 ppm, and 10 ppm. However, @entity4966 could inhibit the expression of @entity10020 protein in a concentration-dependant manner. And the most interestingly, @entity4966 significantly inhibited the cardiac differentiation characterized by the decrease beating percentages. Genes involved in @entity141 signaling pathway were severely disrupted by @entity4966 . CONCLUSION: @entity4966 inhibited the cardiac specific differentiation of @entity1 embryonic stem cell and at the meanwhile the genes responsible for @entity141 signaling pathway were severely disrupted, which could contribute the severe effects of @entity4966 @entity1370 .

[The effects of @entity4965 on cardiac differentiation in XXXX embryonic stem cells and its mechanisms].

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity4966 :: ('CHEBI:16602', 'Chemical') :: ['TCE'] @entity3733 :: ('MESH:D004121', 'Chemical') :: ['dimethyl sulfoxide', 'DMSO'] @entity4965 :: ('MESH:D014241', 'Chemical') :: ['trichloroethylene'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity10020 :: ('7139', 'Gene') :: ['cTnT'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+'] @entity1370 :: ('MESH:D066126', 'Disease') :: ['cardiotoxicity', 'cardiac toxicity']
@entity1 :: (9606,Species) :: ['human']
