BACKGROUND: Dendritic cells (DCs) are potent antigen-presenting cells regarded as crucial in the priming of an immune response. The goal of our study was to test whether bone marrow-generated DCs are capable of inducing protective immunity against a @entity19 @entity0 (4T1). METHODS: DCs were grown from Balb/c @entity19 by culturing lymphocyte-immunodepleted bone marrow in @entity19 granulocyte-macrophage colony-stimulating factor containing medium for 10 days. Balb/c @entity19 (five to eight per group) were immunized intradermally with 1 x 10(6) DCs mixed with 2 x 10(6) lethally irradiated 4T1 cells on day 0. @entity19 in control groups were given intradermal inoculations of @entity1391 -buffered saline solution, 1 x 10(6) DCs, or lethally irradiated 4T1 cells alone. Booster intraperitoneal immunizations of 2 x 10(6) lethally irradiated 4T1 cells were given on days 7 and 14. All @entity19 were challenged with 5 x 10(3) 4T1 cells subcutaneously 7 days after the final immunization. Animals were examined daily, and @entity5 volume was recorded twice weekly with calipers. RESULTS: At 21 days there was a significant reduction in @entity5 growth in @entity19 immunized with DCs mixed with irradiated 4T1 cells as compared with the control groups (p = 0.0005, Kruskal-Wallis test). CONCLUSIONS: These results suggest that DCs mixed with @entity5 cells as a source of undefined @entity5 antigen can induce an effective antitumor immune response. This finding provides a rationale for the use of cultured DCs in immunotherapy of @entity0 .

Active immunization using dendritic cells mixed with XXXX cells inhibits the growth of primary @entity0 .

@entity19 :: ('10090', 'Species') :: ['mice', 'Mice', 'murine'] @entity1391 :: ('CHEBI:26020', 'Chemical') :: ['phosphate'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast and other cancers', 'breast carcinoma'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor']
@entity5 :: (MESH:D009369,Disease) :: ['tumor']
