@entity1 preimplantation development is a critical stage for establishment of the genomic methylation pattern and proper function of the enzymes responsible for this appear essential for normal development. To date, the vast majority of work concerning the developmental expression of the DNA @entity5538 5-methyltansferases (Dnmts) has been conducted in @entity19 . Here we report the sequence and expression of the @entity28319 family during @entity291 preimplantation and fetal development. @entity291 @entity28319 mRNAs display strong sequence homology to those of @entity1 and @entity19 and similar to other species, exist as multiple isoforms. Two of these splice variants, which have been termed Dnmt2gamma and Dnmt3a4, represent previously unreported sequence combinations. Work presented here demonstrates early @entity291 embryos express mRNA coding for the somatic form of @entity28319 and that this transcript fractionates with the ribosome. Unlike the @entity19 model, mRNA encoding the de novo methyltransferases, @entity70484 are present during preimplantation development and can also be found in the ribosomal subcellular fraction. Further, results of Real Time PCR analysis indicate significant differences in @entity28319 mRNA expression levels exist among different tissue types as well as between fetal and adult stages. Recently, it has been postulated that the cause of abnormal methylation observed in cloned embryos may be due in part to misexpression of the Dnmt1o isoform during preimplantation development. Work presented here raises new and significant hypotheses that must be considered both regarding the cadre of DNA methyltranferases that direct epigenetic programming during normal development and regarding the implication of abnormal @entity28319 expression in cloned embryos.

Analysis of DNA ( XXXX 5) methyltransferase mRNA sequence and expression in @entity291 preimplantation embryos, fetal and adult tissues.

@entity1 :: ('9606', 'Species') :: ['human', 'Mammalian'] @entity70484 :: ('13435;13436', 'Gene') :: ['Dnmt3a and 3b'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'murine'] @entity28319 :: ('281119(Tax:9913)', 'Gene') :: ['DNMT', 'Dnmt', 'Dnmt1'] @entity5538 :: ('MESH:D003596', 'Chemical') :: ['cytosine'] @entity291 :: ('9913', 'Species') :: ['Bovine', 'bovine']
@entity5538 :: (MESH:D003596,Chemical) :: ['cytosine']
