UNASSIGNED: @entity1 parturition is associated with many pro-inflammatory mediators which are regulated by the nuclear factor-kappaB (NF-kB) family of transcription factors. In the present study, we employed a ChIP-on-chip approach to define genomic loci within chromatin of PHM1-31 myometrial cells that were occupied by @entity9135 -containing NF-kB dimers in response to a @entity310 stimulation of 1 h. In @entity310 -stimulated PHM1-31 cells, anti- @entity9135 serum enriched 13 300 chromatin regions; importantly, 11 110 regions were also enriched by anti- @entity9135 antibodies in the absence of @entity310 . DNA sequences in these regions, from both unstimulated or @entity310 -stimulated PHM1-31 cultures, were associated with genic regions including @entity4284 , @entity2247 , IL6RN, @entity1178 and @entity50055 . @entity310 -induced binding events at a consensus kB site numbered 1667; these were represented by 112 different instances of the consensus kB motif. Of the 1667 consensus kB motif occurrences, 770 (46.2%) were identified within intronic regions. In unstimulated PHM1-31 cells, anti- @entity9135 -serum-enriched regions were associated with sequences corresponding to open reading frames of ion channel subunit genes including @entity9557 and @entity9402 . Moreover, in unstimulated cells, the consensus kB site was identified 2116 times, being defined by 103 different sequence instances of this motif. Of these 2116 consensus kB motifs, 1089 (51.5%) were identified within intronic regions. Parallel expression array analyses in PHM1-31 cultures demonstrated that @entity310 stimulated a >2-fold induction in 51 genes and a fold repression of >1.5 in 18 others. We identified 14 anti- @entity9135 -serum-enriched genomic regions that correlated with 17 @entity310 -inducible genes, such as @entity2247 , @entity5738 , @entity1178 , @entity4284 and @entity650 , as well as five regions associated with @entity310 -mediated gene repression, including @entity4173 .

Binding loci of XXXX -containing nuclear factor-kappaB dimers in promoter regions of PHM1-31 myometrial smooth muscle cells.

@entity1 :: ('9606', 'Species') :: ['Human'] @entity9135 :: ('5970', 'Gene') :: ['RelA'] @entity650 :: ('3569', 'Gene') :: ['IL6'] @entity9557 :: ('784', 'Gene') :: ['CACNB3'] @entity4173 :: ('1278', 'Gene') :: ['Col1A2'] @entity310 :: ('7124', 'Gene') :: ['TNF'] @entity4284 :: ('4792', 'Gene') :: ['IkBa'] @entity9402 :: ('3745', 'Gene') :: ['KCNB1'] @entity1178 :: ('3725', 'Gene') :: ['Jun'] @entity5738 :: ('1958', 'Gene') :: ['Egr-1'] @entity50055 :: ('27094', 'Gene') :: ['KCNMB3'] @entity2247 :: ('4513', 'Gene') :: ['COX-2', 'COX2']
@entity9135 :: (5970,Gene) :: ['RelA']
