It was evaluated whether @entity179 is apoptosis and occurs with alterations in @entity7425 -linked metabotropic @entity2227 receptors (mGluRs) and their associated signaling pathways. A @entity235 model of @entity2806 was used. The @entity52917 pyramidal cells and cerebellar Purkinje cells underwent progressive @entity528 . By in situ end-labeling of DNA, death of @entity52917 and Purkinje cells was greater at 7 days than 1 day after @entity63 , whereas death of granule neurons in dentate gyrus and cerebellar cortex was greater at 1 than at 7 days. Ultrastructurally, degenerating @entity52917 pyramidal neurons and cerebellar Purkinje cells were @entity39 ; in contrast, degenerating granule neurons were apoptotic. In agarose gels of regional DNA extracts, random DNA fragmentation coexisted with internucleosomal fragmentation. By immunoblotting of regional homogenates, mGluR1alpha, @entity10092 , phospholipase Cbeta (PLCbeta), and Galphaq/11 protein levels in hippocampus at 1 and 7 days after @entity63 were similar to control levels, but in cerebellar cortex, mGluR1alpha and @entity10092 were decreased but PLCbeta was increased. By immunocytochemistry, mGluR and PLCbeta immunoreactivity dissipated in @entity52917 and cerebellar Purkinje cell/ molecular layers, whereas immunoreactivities for these proteins were enhanced in granule neurons. It was concluded that @entity528 after global @entity63 exists as two distinct, temporally overlapping forms in hippocampus and cerebellum: @entity39 of pyramidal neurons and Purkinje cells and apoptosis of granule neurons. @entity528 is associated with a loss of @entity7425 -linked mGluR transduction proteins, whereas neuronal apoptosis occurs with increased mGluR signaling.

Apoptosis and @entity39 occur in separate neuronal populations in hippocampus and cerebellum after XXXX and are associated with differential alterations in metabotropic @entity2227 receptor signaling pathways.

@entity2227 :: ('CHEBI:29987', 'Chemical') :: ['glutamate'] @entity2806 :: ('MESH:D002545', 'Disease') :: ['transient global incomplete cerebral ischemia'] @entity7425 :: ('CHEBI:18179', 'Chemical') :: ['phosphoinositide'] @entity235 :: ('9615', 'Species') :: ['dog'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemia'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrotic', 'necrosis'] @entity52917 :: ('487031(Tax:9615)', 'Gene') :: ['CA1'] @entity10092 :: ('14805(Tax:10090)', 'Gene') :: ['mGluR5'] @entity179 :: ('MESH:D019636', 'Disease') :: ['postischemic neurodegeneration'] @entity528 :: ('MESH:D009410', 'Disease') :: ['delayed degeneration', 'neuronal death', 'Neuronal necrosis']
@entity63 :: (MESH:D007511,Disease) :: ['ischemia']
