@entity2842 ( @entity2842 ) is important in attracting monocytes to sites of @entity32 . Using predominantly pharmacological approaches, prior studies have indicated that @entity563 / @entity1889 kinases are involved in the @entity2842 -induced signaling pathways. We report here that there is substantial inhibition of @entity2842 -stimulated chemotaxis of @entity1 monocytes treated with inhibitors selective for the subset of @entity563 / @entity1889 kinases, @entity7131 ( @entity7131 ). Selective inhibitors of @entity7131 such as @entity11009 and Calphostin C both caused approximately 80% inhibition of chemotaxis. Because these pharmacological inhibitors do not specifically inhibit individual @entity7131 isoforms, we chose to use antisense oligodeoxyribonucleotides (ODN) to specifically reduce @entity7131 isoform expression, first by inhibiting expression of the conventional @entity7131 family, and next by using specific antisense ODN for @entity10185 and @entity23001 . Conventional @entity7131 -antisense ODN treatment completely and significantly inhibited monocyte chemotaxis to @entity2842 , whereas sense-control ODN caused no significant inhibition. @entity23001 -antisense ODN caused 89.2% inhibition of chemotaxis at its highest dose. In contrast, @entity23001 -sense ODN and @entity10185 -antisense and -sense ODN were without effect. Further studies evaluating the @entity460 response that is triggered upon @entity2842 interaction with its receptor, @entity9172 , indicate that this response is not altered by antisense or sense ODN treatment, thus supporting our hypothesis that @entity23001 is critical for post-receptor signal transduction downstream of the immediate @entity460 signal. These data contribute to our developing understanding of the signal transduction pathways involved in the chemotactic response of @entity1 monocytes to @entity2842 and uniquely identify the requirement for the @entity23001 isoform in this important process.

@entity23001 is required for @entity1 monocyte chemotaxis to XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity23001 :: ('5579', 'Gene') :: ['PKCbeta'] @entity9172 :: ('729230', 'Gene') :: ['CCR2'] @entity7131 :: ('MESH:D020151', 'Disease') :: ['protein kinase C', 'PKC'] @entity2842 :: ('6347', 'Gene') :: ['MCP-1', 'Monocyte chemoattractant protein 1'] @entity11009 :: ('MESH:C070515', 'Chemical') :: ['GF109203X'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity563 :: ('CHEBI:17822', 'Chemical') :: ['serine'] @entity1889 :: ('CHEBI:26986', 'Chemical') :: ['threonine'] @entity10185 :: ('5578', 'Gene') :: ['PKCalpha']
@entity2842 :: (6347,Gene) :: ['MCP-1']
