The aim of this study was to evaluate a novel third-generation enzyme-linked immunosorbent assay (ELISA) for the high-sensitivity detection of autoantibodies to @entity7134 ( @entity7134 ) in @entity1 with @entity2883 ( @entity2883 ). First- and second-generation ELISA for the detection of antineutrophil cytoplasmic antibodies (ANCA) frequently demonstrate insufficient sensitivity due to inadequate presentation of autoantigenic epitopes. @entity1 @entity7134 was immobilized on the solid phase of ELISA plates by anchoring technique. Anti- @entity7134 reactivity was measured in 34 C-ANCA positive @entity1 with @entity2883 , 11 MPO-ANCA-positive @entity1 with other @entity792 , 65 @entity1 with @entity1252 ( @entity1252 ), and 137 healthy blood donors. Thirty-three of 34 @entity1 with @entity2883 (97.1%) showed positive anti- @entity7134 IgG antibody reactivity. None of 11 MPO-ANCA positive @entity1333 @entity1 , none of 137 blood donors, and 3 of 65 @entity1252 @entity1 expressed elevated IgG reactivity to @entity7134 (specificity: 98.4%). Comparison with another third-generation ELISA did not reveal different qualitative results. However, there was no significant correlation between quantitative results of both assays. Receiver operating characteristic (ROC) curve analysis revealed a significantly better assay performance compared with first (direct)- and second (capture)-generation assays (P = 0.011 and P = 0.001, respectively). Third-generation (anchor) anti- @entity7134 ELISA exhibit significantly higher sensitivity than previous generation assays. Anchoring of @entity7134 renders the granulocyte protein more autoantigenic compared with direct or capture immobilization.

High-sensitivity detection of autoantibodies against XXXX by a novel third-generation enzyme-linked immunosorbent assay.

@entity1 :: ('9606', 'Species') :: ['patients', 'Human'] @entity1333 :: ('MESH:D014657', 'Disease') :: ['vasculitis'] @entity1252 :: ('MESH:D008180', 'Disease') :: ['SLE', 'systemic lupus erythematosus'] @entity2883 :: ('MESH:D014890', 'Disease') :: ['WG', "Wegener's granulomatosis"] @entity7134 :: ('5657', 'Gene') :: ['proteinase-3', 'PR3'] @entity792 :: ('MESH:D001327', 'Disease') :: ['autoimmune vasculitides']
@entity7134 :: (5657,Gene) :: ['proteinase-3']
