@entity2946 ( @entity2946 ), the most common pediatric @entity1313 , is a highly malignant disease with a 5-year survival rate of only 60%. @entity5 cells invade surrounding tissue and disseminate through cerebral spinal fluid, making treatment difficult. @entity1 @entity5765 exploits an activated Ras pathway in @entity5 cells to support productive infection as an oncolytic virus. Here, we examined the ability of @entity1 reovirus to kill @entity2946 cells lines and surgical specimens in vitro and inhibit @entity5 growth/ @entity3 in vivo. Most @entity1 @entity2946 cell lines tested (five of seven = 71.4%), two @entity2946 cell lines derived from spontaneously arising @entity5 in Patched-1(+/-) @entity19 (two of two = 100%) and three @entity2946 primary cultures derived from surgical specimens, were susceptible to @entity7697 . Reovirus was internalized and transcribed in both susceptible and resistant cell lines. However, viral protein synthesis was restricted to cell lines with higher levels of activated Ras, suggesting that Ras plays a critical role in reovirus oncolysis in @entity2946 . Using an in vivo Daoy orthotopic animal model, we found that a single i.t. injection of reovirus dramatically prolonged survival compared with controls (160 versus 70 days, respectively; P = 0.0003). Repeating this experiment with GFP-labeled @entity1 cells and multiple i.t. administrations of reovirus, we again found prolonged survival and a dramatic reduction in spinal and leptomeningeal @entity3 (66.7% in control injections versus 0.0% in the live virus group). These data suggest that this oncolytic virus may be a potentially effective novel therapy against @entity1 @entity2946 . Its ability to reduce @entity3 to the spinal cord could allow a reduction in the dose/field of total neuroaxis cerebral-spinal radiotherapy currently used to treat/prevent cerebral spinal fluid dissemination.

Reovirus prolongs survival and reduces the frequency of spinal and leptomeningeal XXXX from medulloblastoma.

@entity1 :: ('9606', 'Species') :: ['human', 'Daoy', 'Human'] @entity1313 :: ('MESH:D001932', 'Disease') :: ['brain tumor'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'Tumor', 'tumors'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity5765 :: ('538123', 'Species') :: ['reovirus type 3'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastases'] @entity7697 :: ('MESH:D012088', 'Disease') :: ['reovirus infection'] @entity2946 :: ('MESH:D008527', 'Disease') :: ['Medulloblastoma', 'MB']
@entity3 :: (MESH:D009362,Disease) :: ['metastases']
