Here we investigated the in vivo effect of morin (500ppm in diet) in fostering apoptosis in @entity107 ( @entity108 ) (200mg/kg bodyweight) mediated experimental hepatocellular @entity105 model. We analyzed the expression of cytosolic protein @entity584 and their important apoptotic downstream targets like @entity8187 , @entity588 , @entity589 , GSK-3betain vivo, by immunoblot analysis. In silico docking studies indicated that morin could serve as a better inhibitor than the classical PI3K inhibitor @entity2306 . The results obtained from in vivo studies confirm this. We also demonstrate here that morin's interaction with a defined set of @entity1522 of PI3K p110gamma catalytic subunit resulted in the down-regulation of p- @entity584 (Ser473), p- @entity584 (Thr308) and total @entity584 causing the attenuation of its downstream targets in @entity108 -induced @entity157 . Further, @entity70261 caused the up-regulation of @entity5 suppressor @entity983 , an important negative regulator of @entity584 , thus initiating apoptosis. Supplementation of morin to experimental animals modulated @entity588 / @entity589 ratio causing the release of cyt C and up-regulation of @entity9189 . Morin was also found to prevent the @entity584 -mediated suppression of @entity9459 possibly causing cell cycle arrest at the G1/S phase. These observations were supported by the DNA fragmentation and transmission electron microscopy results, which showed the occurrence of apoptosis. In conclusion, our findings demonstrate that morin begets apoptosis in @entity108 -induced @entity157 .

XXXX fosters apoptosis in experimental hepatocellular @entity105 model.

@entity9189 :: ('836;842', 'Gene') :: ['caspase-3 and -9'] @entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity108 :: ('CHEBI:34873', 'Chemical') :: ['DEN'] @entity589 :: ('581', 'Gene') :: ['Bax'] @entity588 :: ('596', 'Gene') :: ['Bcl-2'] @entity70261 :: ('MESH:C008548', 'Chemical') :: ['morin'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity107 :: ('MESH:D004052', 'Chemical') :: ['diethylnitrosamine'] @entity584 :: ('207', 'Gene') :: ['Akt'] @entity8187 :: ('842', 'Gene') :: ['caspase-9'] @entity2306 :: ('MESH:C085911', 'Chemical') :: ['LY294002'] @entity983 :: ('5728', 'Gene') :: ['PTEN'] @entity157 :: ('MESH:D006528', 'Disease') :: ['hepatocellular carcinoma'] @entity105 :: ('MESH:D063646', 'Disease') :: ['carcinogenesis'] @entity9459 :: ('2932', 'Gene') :: ['GSK-3beta']
@entity70261 :: (MESH:C008548,Chemical) :: ['Morin']
