To develop a molecular-based assay so that the diagnosis of feline @entity1892 can be facilitated, we have characterized 24 feline immunoglobulin heavy chain variable region ( @entity7513 ) complementary DNA (cDNA) transcripts. Structural homology with rearranged @entity1 @entity7513 genes was found, and the sequence information was used to design a feline-specific polymerase chain reaction (PCR)-based assay to amplify the complementarity determining region 3 as a marker for B-cell clonality. Conserved primers derived from the second and third framework regions of V gene segments were used in conjunction with 2 sequence-specific primers and 1 degenerate primer derived from the J gene segments. Each PCR reaction was run in duplicate, and both native and denatured PCR products were evaluated using @entity2975 gel electrophoresis. @entity406 -fixed, @entity982 -embedded (FFPE) tissue sections from @entity468 with confirmed @entity1892 (diffuse large @entity1892 , @entity6872 , and @entity1582 ) were examined, and 15/22 (68.2%) @entity468 produced results indicative of the presence of a monoclonal population of B cells. The evaluation of denatured PCR products (heteroduplex analysis) facilitated a more accurate interpretation in 3/15 (20%) @entity468 . Pseudoclonality was a major reason for the failure to detect monoclonality. Poor DNA quality is a significant concern and was responsible for the removal of 2 @entity468 from the study. Using this assay, FFPE normal feline lymphoid tissues and unfixed peripheral blood mononuclear cells were determined to be composed of polyclonal populations of B cells. This assay represents a useful adjunctive diagnostic tool for the diagnosis and investigation of feline B-cell @entity1071 .

Characterization of feline immunoglobulin heavy chain variable region genes for the molecular diagnosis of XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity1071 :: ('MESH:D008232', 'Disease') :: ['lymphoproliferative disorders'] @entity6872 :: ('MESH:D010954', 'Disease') :: ['plasmacytoma'] @entity406 :: ('MESH:D005557', 'Chemical') :: ['Formalin'] @entity1582 :: ('MESH:D009101', 'Disease') :: ['myeloma'] @entity1892 :: ('MESH:D016393', 'Disease') :: ['B-cell lymphoma', 'B-cell neoplasia'] @entity982 :: ('MESH:D010232', 'Chemical') :: ['paraffin'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity468 :: ('9685', 'Species') :: ['cats'] @entity7513 :: ('3492', 'Gene') :: ['IGH V']
@entity1892 :: (MESH:D016393,Disease) :: ['B-cell neoplasia']
