OBJECTIVE: Platelet activation was assessed in @entity1 with @entity3657 compared with healthy control subjects. METHODS: This prospective comparative study included 100 subjects: 40 consecutive @entity1 with @entity613 , 20 consecutive @entity1 with critical @entity63 and tissue loss, and 40 healthy control subjects. Whole blood flow cytometric analysis was performed to determine resting and stimulated platelet @entity8729 expression and resting and stimulated platelet @entity2002 binding. Results are presented as platelet percentage and also as mean fluorescence intensity. RESULTS: @entity8729 expression was significantly increased in @entity1 with @entity613 (median, 0.85%; range, 0.31%-4.77%; P =.023) and critical @entity63 (median, 1.11%; range, 0.2%-3.26%; P =.028) compared with control subjects (median, 0.59%; range, 0.16%-4.58%). The percentage of platelets binding @entity2002 was also significantly higher in @entity1 with @entity613 (median, 2.89%; range, 1.08%-9.59%; P <.001) compared with control subjects (median, 1.57%; range, 0.17%-10.7%). There was no significant difference in percentage of platelet @entity2002 binding between control subjects and @entity1 with critical @entity63 . @entity2002 binding by stimulated platelets was significantly diminished in @entity1 with @entity63 compared with control subjects (67.2% vs 77.9%; P =.006). CONCLUSIONS: Platelet activation is increased in @entity1 with @entity3657 , suggesting an underlying prothrombotic state. Platelets from @entity1 with @entity63 are less responsive to in vitro stimulation.

Platelet activation is increased in XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity2002 :: ('2244', 'Gene') :: ['Fibrinogen', 'fibrinogen'] @entity3657 :: ('MESH:D058729', 'Disease') :: ['peripheral arterial disease'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemia', 'critical limb ischemia'] @entity8729 :: ('6403', 'Gene') :: ['P-selectin'] @entity613 :: ('MESH:D007383', 'Disease') :: ['intermittent claudication']
@entity3657 :: (MESH:D058729,Disease) :: ['peripheral arterial disease']
