BACKGROUND: Anopheles stephensi is an important urban @entity311 vector in the Indian subcontinent. Extensive application of insecticides evokes micro-evolution which results in resistance that can be traced back to their genotypes. In this study, resistant and susceptible strains of An. stephensi for @entity5179 were selected by selective inbreeding for 27 and 10 generations respectively. Biochemical basis of resistance in all the life stages is investigated. Quantitative assays were performed for proteins (total and soluble), esterases (a, b and @entity934 ) and phosphatases (acid and alkaline) by spectrophotometry and qualitative assays for the enzymes by native @entity2975 gel electrophoresis. RESULTS: The enzyme quantities significantly varied in all the life stages of resistant strain as compared to the susceptible ones. Qualitative studies showed 7 isoforms for a and b esterases, 3 each for acetylcholinesterase and alkaline phosphatase and 2 for acid phosphatase. Exclusive bands were found in resistant strain like a- @entity4912 and b- @entity4912 in eggs and larvae, b-Est 3 in adult males, b- @entity2215 in adult females, AlkP 1, AlkP 2 and AlkP 3 in adult females, larvae and adult males respectively. CONCLUSION: Variations in the quantity and specific enzyme isoforms play key role in the development of @entity5179 resistance in An. stephensi.

Biochemical basis of XXXX resistance in different life stages of Anopheles stephensi strains of Bangalore, India.

@entity4912 :: ('6783', 'Gene') :: ['Est 1'] @entity5179 :: ('MESH:C017160', 'Chemical') :: ['alphamethrin'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity311 :: ('MESH:D008288', 'Disease') :: ['malaria'] @entity2215 :: ('7015', 'Gene') :: ['Est 2'] @entity934 :: ('MESH:D000109', 'Chemical') :: ['acetylcholine']
@entity5179 :: (MESH:C017160,Chemical) :: ['alphamethrin']
