We recently developed a short-term assay for skin @entity274 generation induced by double @entity864 ( @entity865 ) applications for mechanistic study on skin epidermal @entity105 . In the present study, we investigated the individual roles of @entity1426 metabolism in @entity274 generation in @entity19 @entity32 . The experiments using inhibitors of @entity1426 (AA) metabolism showed that @entity228 and a lipoxygenase (LO) inhibitor expectedly suppressed double @entity865 application-induced @entity274 generation through the interference of chemotactic action but not by direct decomposition or scavenging. We also demonstrated that the treatment of AA (1 mumol) and 5-LO metabolites including leukotriene B4 (LTB4) partly mimicked, though @entity1460 LO-derived lipid @entity273 and @entity2918 did not, the priming effect evaluated by @entity4 formation and leukocyte infiltration. We also confirmed that inflammatory leukocytes accumulated by LTB4 generated a significant amount of @entity274 by @entity865 stimulation. These results suggested that 5-LO metabolites of AA are the potential key molecules in the @entity865 -induced priming event. Interestingly, the @entity10178 -selective inhibitor @entity9695 (NS) and @entity12698 (CXB) showed different responses than those of other inhibitors. These agents showed no specific potential to inhibit the priming event but significantly suppressed @entity274 generation, lipid peroxidation, and @entity1373 in @entity19 skin. From the results based on an in vitro leukocyte differentiation model, we speculated that the antioxidant effect of the @entity10178 inhibitors might be partly associated with both counteraction of proinflammatory cytokine-enhanced ROS generation and inhibition of @entity3804 , an important molecule for cell adhesion, expression. Indeed, the topical application of NS attenuated the number of infiltrated leukocytes induced by @entity865 in @entity19 skin. Thus, these gathered data indicated the differential roles of 5-LO and @entity10178 in leukocyte adhesion, infiltration, and @entity274 generation.

XXXX cascade inhibitors modulate phorbol ester-induced oxidative stress in female ICR @entity19 skin: differential roles of 5-lipoxygenase and cyclooxygenase-2 in leukocyte infiltration and activation.

@entity32 :: ('MESH:D007249', 'Disease') :: ['skin inflammation'] @entity10178 :: ('17709(Tax:10090)', 'Gene') :: ['COX-2', 'cyclooxygenase (COX-) 2'] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H2O2'] @entity12698 :: ('MESH:C105934', 'Chemical') :: ['celecoxib'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity4 :: ('MESH:D004487', 'Disease') :: ['edema'] @entity1373 :: ('MESH:D006965', 'Disease') :: ['hyperplasia'] @entity228 :: ('CHEBI:50858', 'Chemical') :: ['corticosteroid'] @entity1426 :: ('MESH:D016718', 'Chemical') :: ['arachidonic acid'] @entity2918 :: ('MESH:D011453', 'Chemical') :: ['prostaglandins'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['hydroperoxide'] @entity865 :: ('CHEBI:61432', 'Chemical') :: ['TPA'] @entity105 :: ('MESH:D063646', 'Disease') :: ['carcinogenesis'] @entity1460 :: ('3847', 'Species') :: ['soybean'] @entity9695 :: ('MESH:C012655', 'Chemical') :: ['nimesulide'] @entity3804 :: ('16409(Tax:10090)', 'Gene') :: ['CD11b'] @entity864 :: ('MESH:C554292|MESH:D013755', 'Chemical') :: ['12-O-tetradecanoylphorbol-13-acetate']
@entity1426 :: (MESH:D016718,Chemical) :: ['Arachidonic acid']
