UNASSIGNED: 97 Background: Multidrug resistance (MDR) phenotype is the major cause of chemotherapy failure. It has been related to several proteins expression: @entity1262 ( @entity1262 ), MRP family, @entity8993 , and @entity28671 . We study the resistance pattern induced after @entity1311 and @entity3003 in @entity1149 ( @entity1149 ). METHODS: We used 5 different cell lines. A549 and @entity1 cell lines were derived from lung @entity189 . NODO line was obtained from @entity2 metastases, and 3 cell lines derived from other @entity5 : @entity356 ( @entity356 ), @entity4797 ( @entity4797 ), @entity0 (MCF7). The cell lines were exposed to @entity1311 , @entity3003 , and the combination of both drugs. The concentrations we used were similar to the plasma concentrations observed in @entity1 treated previously. RESULTS: A549 cell line did not show basal expression of @entity1262 , but a weak basal expression of @entity22149 was observed. After chemotherapy, we observed that @entity1311 induced @entity1262 and @entity3003 induced @entity22149 . The combination of both drugs induced @entity1262 and @entity22149 expression, however, when @entity1311 is associated to @entity3003 , @entity22149 expression did not increase significantly. @entity1 cell line did not show basal expression of @entity1262 and @entity22149 . @entity1311 induced @entity1262 and @entity3003 induced @entity22149 . The association of both drugs increased significantly the expression of @entity1262 , and very few the expression of @entity22149 . NODO cell line did not show any basal expression. @entity1311 induced @entity1262 and @entity3003 induced @entity22149 . Cell lines derived from other @entity5 not show any basal expression. @entity1311 induced @entity1262 in all cell lines. @entity3003 did not induce @entity1262 , nor @entity22149 in @entity356 cell line. CONCLUSIONS: Plasma concentrations of @entity1311 induced @entity1262 expression but not @entity22149 in @entity2 and other @entity5 derived cell lines. @entity3003 produced overexpression of @entity22149 but not @entity1262 in the same cell lines. The combination of both drugs at plasma concentrations was not able to activate a new resistance mechanism in the studied cell lines, but improve the resistance mechanism induced by each one of the drugs individually.

Drug resistance induced by XXXX and @entity3003 plasmatic concentrations in @entity2 cell lines.

@entity1 :: ('9606', 'Species') :: ['A427', 'patients'] @entity1262 :: ('5243', 'Gene') :: ['PgP', 'MDR1', 'P-glycoprotein'] @entity28671 :: ('644079', 'Gene') :: ['BCRP'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity22149 :: ('8714', 'Gene') :: ['MRP3'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumours'] @entity8993 :: ('4035', 'Gene') :: ['LRP'] @entity3003 :: ('MESH:D016190', 'Chemical') :: ['Carboplatin', 'carboplatin'] @entity1149 :: ('MESH:D002289', 'Disease') :: ['non-small cell lung cancer', 'NSCLC'] @entity1311 :: ('MESH:D017239', 'Chemical') :: ['Paclitaxel', 'paclitaxel'] @entity189 :: ('MESH:D002277', 'Disease') :: ['epithelial carcinoma'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung carcinoma', 'lung cancer'] @entity4797 :: ('MESH:D018239', 'Disease') :: ['SEM', 'seminoma'] @entity356 :: ('MESH:D013274', 'Disease') :: ['GAS', 'gastric cancer']
@entity1311 :: (MESH:D017239,Chemical) :: ['paclitaxel']
