OBJECTIVE: To investigate the underlying mechanism for the therapeutic effect of a traditional Chinese medicinal recipe, Antidotal and Myogenic Ointment ( @entity21663 ), on the foot @entity1066 in @entity6 @entity35 using cDNA microarray technology. METHODS: 45 @entity35 were made @entity6 by i. p. injection of @entity3839 . The treated animals were then fed for 6 months,and subjected to the dissection of distal popliteal artery after ligation of the vessels. Another month later, grade @entity130 was produced on the bottom of their foot as a model of @entity4760 . The @entity35 were then randomly divided into three groups (15 each) to receive @entity21663 , @entity8160 ( @entity8160 ) and saline for 30 days, with dressing change in every 2 days. The area of @entity1066 wound and their healing rate were recorded before and after the treatment. Total RNA was extracted from the tissue samples collected near the wound, and the expression profile of cytokine genes demonstrated using the microarry for @entity35 . RESULTS: In comparison with the saline group, difference in the level of expression was found in 25 genes (23 of them were up-regulated and 2 down-regulated) in @entity8160 group, and 30 genes in @entity21663 groups (29 of them up-regulated and 1 down-regulated ). In comparison with @entity8160 group, difference in level of expression was found in 16 genes in @entity21663 group, with 11 up-regulated and 5 down-regulated. Neurotrophic factors and chemotactic factors, etc were among the genes involved. CONCLUSION: In comparison with @entity8160 , @entity21663 is more effective in the treatment of foot @entity1066 in @entity6 @entity35 . It is possible that @entity21663 produces such effects through the regulation of balance in cytokine expression.

[A microarray study on the molecular mechanism for the therapeutic effect of Antidotal and Myogenic Ointment on the foot XXXX in @entity6 @entity35 ].

@entity8160 :: ('25313(Tax:10116)', 'Gene') :: ['epidermal growth factor', 'EGF'] @entity21663 :: ('CHEBI:53705', 'Chemical') :: ['AMO'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetic'] @entity1066 :: ('MESH:D014456', 'Disease') :: ['ulcer'] @entity3839 :: ('MESH:D013311', 'Chemical') :: ['streptozocin'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity130 :: ('MESH:D014947', 'Disease') :: ['II burn injury'] @entity4760 :: ('MESH:D017719', 'Disease') :: ['diabetic foot ulcer']
@entity1066 :: (MESH:D014456,Disease) :: ['ulcer']
