OBJECTIVES: Eosinophilic esophagitis (EoE) is a chronic, antigen-mediated disease in @entity1 and adults associated with substantial esophageal remodeling and fibrosis. The expression of the remodeling-associated matrix metalloproteinases (MMPs) has not been previously detailed in EoE. METHODS: @entity68748 expression and cellular localization were assessed using real-time quantitative polymerase chain reaction and immunohistochemistry/immunofluorescence in EoE fibroblasts, active and inactive pediatric EoE biopsies, and nondiseased control biopsies. The effect of transforming growth factor (TGF)-b1 treatment on @entity1476 expression in cultured esophageal epithelial ( @entity1 ) cells was analyzed. RESULTS: @entity68748 mRNA were expressed in EoE fibroblasts and biopsies. Proliferating epithelial cells produced MMP-14 more abundantly in EoE than in controls (P<0.001) and the degree of epithelial MMP-14 expression correlated positively with basal zone hyperplasia (r=0.65, P=0.002). EoE lamina propria had higher numbers of @entity1476 - and -14-positive cells (906  167 and 701  93cells/mm) as compared with controls (258  93cells/mm, P<0.01 and 232  54cells/mm, P<0.01), and MMP-14 expression correlated with the severity of fibrosis. Following therapy with topical @entity288 , @entity68749 were significantly diminished (P<0.01). @entity2533 increased the expression and secretion of @entity1476 from esophageal epithelial @entity1 cells. CONCLUSIONS: @entity68748 are elevated in pediatric @entity1 with EoE and significantly decrease following topical corticosteroid therapy. @entity2533 increases @entity1476 in esophageal epithelial cells. This alludes to previously unappreciated role for MMPs in EoE-associated esophageal remodeling and a potential positive feedback loop via @entity2533 .

XXXX Are Elevated in Eosinophilic Esophagitis and Reduced Following Topical Corticosteroid Therapy.

@entity1 :: ('9606', 'Species') :: ['children', 'patients', 'HET1A'] @entity1476 :: ('4313', 'Gene') :: ['MMP-2'] @entity2533 :: ('7040', 'Gene') :: ['TGF-b1'] @entity68748 :: ('4313;4323', 'Gene') :: ['MMP-2 and -14'] @entity68749 :: ('4323;4313', 'Gene') :: ['MMP-14 and -2'] @entity288 :: ('MESH:D000305', 'Chemical') :: ['corticosteroids']
@entity68748 :: (4313;4323,Gene) :: ['MMPs-2 and -14']
