OBJECTIVES: The aim of this study was to determine the occurrence of @entity25323 ( @entity25323 )-producing @entity526 at an @entity1641 clinic and a horseback riding centre, and to discuss the impact of antimicrobial treatment on resistance selection. METHODS: Faeces from @entity1641 , environmental smears and flies were sampled at both the clinic and riding centre. Staff at the @entity1641 clinic were also examined. The samples were cultivated on MacConkey agar with @entity3524 (2 mg/L) to isolate @entity25323 -producing @entity526 . The presence of bla and qnr genes was tested by PCR, and transferability was determined by conjugation. Replicon typing and restriction analysis of plasmids harbouring @entity25323 and qnr genes were performed. RESULTS: @entity526 with the blaCTX-M-1 gene were isolated from @entity1641 , staff, environmental smears and flies at the two sites. @entity526 isolates from the @entity1641 clinic harboured an IncHI1 conjugative 235-285 kb plasmid containing blaCTX-M-1, catA1, strA, sul2 and tet(B) genes. Some of these were positive for @entity14723 and/or @entity14724 , and were located on 40 or 45 kb IncN or IncX1 conjugative plasmids. The gene blaCTX-M-1 in isolates from the riding centre was carried by IncN (30 kb) and IncI1 (85 kb) conjugative plasmids. Horizontal gene transfer seems to be involved in disseminating @entity526 with @entity25323 and qnr genes at the clinic and riding centre. CONCLUSIONS: The study illustrates that @entity25323 -producing @entity526 , as well as plasmids carrying @entity25323 genes of clinical interest, can be easily transferred among @entity1641 , @entity1 and flies living in close contact.

Plasmids carrying blaCTX-M-1 and qnr genes in XXXX isolates from an @entity1641 clinic and a horseback riding centre.

@entity1 :: ('9606', 'Species') :: ['humans'] @entity1641 :: ('9796', 'Species') :: ['horses', 'equine'] @entity14723 :: ('20467117', 'Gene') :: ['qnrS1'] @entity14724 :: ('13906642', 'Gene') :: ['qnrB19'] @entity3524 :: ('MESH:D002439', 'Chemical') :: ['cefotaxime'] @entity25323 :: ('13906541', 'Gene') :: ['extended-spectrum b-lactamase', 'ESBL'] @entity526 :: ('562', 'Species') :: ['Escherichia coli', 'E. coli']
@entity526 :: (562,Species) :: ['Escherichia coli']
