PURPOSE: We aimed to investigate the methylation pattern in @entity150 and assess the diagnostic potential of such epigenetic changes in urine. EXPERIMENTAL DESIGN: The methylation status of 7 genes ( @entity4882 , @entity2529 , @entity5962 , @entity986 , @entity9288 , @entity4191 , and @entity8724 ) in 98 cases of @entity150 and 4 cases of @entity2735 was analyzed by methylation-specific PCR. Twenty-two cases had paired voided urine samples for analysis. RESULTS: In transitional cell @entity5 tissues, aberrant methylation was frequently detected in @entity4882 (87.8%), @entity2529 (58.2%), @entity5962 (63.3%), and @entity986 (26.5%), whereas methylation of @entity9288 (13.3%), @entity4191 (5.1%), and @entity8724 (5.1%) is not common. No association between methylation status and grading or muscle invasiveness was demonstrated. In 22 paired voided urine samples of @entity150 , methylation of @entity2529 , @entity4882 , @entity5962 , and @entity986 could be detected in 45.5%, 68.2%, 59.1%, and 13.6% of the cases, respectively. The sensitivity of methylation analysis (90.9%) was higher than that of urine cytology (45.5%) for @entity5 detection. Methylation of @entity4882 (50%), @entity2529 (75%), and @entity5962 (50%) was also detected in @entity2735 . In 7 normal urothelium samples and 17 normal urine controls, no aberrant methylation was detected except for @entity4882 methylation in 3 normal urothelium samples (42.9%) and 4 normal urine samples (23.5%), respectively. CONCLUSIONS: Our results demonstrated a distinct methylation pattern in @entity150 with frequent methylation of @entity4882 , @entity2529 , @entity5962 , and @entity986 . Detection of gene methylation in routine voided urine using selected markers appeared to be more sensitive than conventional urine cytology.

Hypermethylation of multiple genes in XXXX tissues and voided urine in @entity150 patients.

@entity2529 :: ('1612', 'Gene') :: ['DAPK'] @entity2735 :: ('MESH:D002278', 'Disease') :: ['carcinoma in situ'] @entity986 :: ('1029', 'Gene') :: ['p16'] @entity5 :: ('MESH:D009369', 'Disease') :: ['carcinoma tumor', 'cancer'] @entity9288 :: ('1030', 'Gene') :: ['p15'] @entity5962 :: ('999', 'Gene') :: ['E-cadherin'] @entity8724 :: ('4255', 'Gene') :: ['MGMT'] @entity150 :: ('MESH:D001749', 'Disease') :: ['bladder transitional cell carcinoma', 'bladder cancer'] @entity4191 :: ('2950', 'Gene') :: ['GSTP1'] @entity4882 :: ('5915', 'Gene') :: ['RARbeta']
@entity5 :: (MESH:D009369,Disease) :: ['tumor']
