UNASSIGNED: Conclusion The findings demonstrated the potential use of induced pluripotent stem cells for regeneration of tracheal epithelium. Objective Autologous tissue implantation techniques using skin or cartilage are often applied in cases of @entity8276 with laryngeal inflammatory lesions and @entity3 . However, these techniques are invasive with an unstable clinical outcome. The purpose of this study was to investigate regeneration in a tracheal defect site of nude @entity35 after implantation of ciliated epithelium that was differentiated from induced pluripotent stem cells. Method Embryoid bodies were formed from @entity19 induced pluripotent stem cells. They were cultured with growth factors for 5 days, and then cultured at the air-liquid interface. The degree of differentiation achieved prior to implantation was determined by histological findings and the results of real-time polymerase chain reaction. Embryoid bodies including ciliated epithelium were embedded into collagen gel that served as an artificial scaffold, and then implanted into nude @entity35 , creating an 'air-liquid interface model'. Histological evaluation was performed 7 days after implantation. Results The ciliated epithelial structure survived on the lumen side of regenerated tissue. It was demonstrated histologically that the structure was composed of ciliated epithelial cells.

Regeneration of tracheal epithelium using XXXX induced pluripotent stem cells.

@entity35 :: ('10116', 'Species') :: ['rats'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity3 :: ('MESH:D009362', 'Disease') :: ['malignant tumor invasion'] @entity8276 :: ('MESH:D014133', 'Disease') :: ['tracheal defects']
@entity19 :: (10090,Species) :: ['mouse']
