Neuroinflammation is characterized by activation of microglial cells, followed by production of @entity842 (NO), which may have different outcomes on neurogenesis, favoring or inhibiting this process. In the present study, we investigated how the inflammatory mediator NO can affect proliferation of neural stem cells (NSCs), and explored possible mechanisms underlying this effect. We investigated which mechanisms are involved in the regulation of NSC proliferation following treatment with an inflammatory stimulus (lipopolysaccharide plus IFN-y), using a culture system of subventricular zone (SVZ)-derived NSCs mixed with microglia cells obtained from wild-type @entity19 (iNOS(+/+)) or from iNOS knockout @entity19 (iNOS(-/-)). We found an impairment of NSC cell proliferation in iNOS(+/+) mixed cultures, which was not observed in iNOS(-/-) mixed cultures. Furthermore, the increased release of NO by activated iNOS(+/+) microglial cells decreased the activation of the ERK/MAPK signaling pathway, which was concomitant with an enhanced nitration of the EGF receptor. Preventing @entity1568 reactive species formation with @entity42043 , a scavenger of @entity6710 ( @entity29101 ), or using the @entity29101 degradation catalyst FeTMPyP, cell proliferation and ERK signaling were restored to basal levels in iNOS(+/+) mixed cultures. Moreover, exposure to the NO donor @entity45337 (100  M), for 48 h, inhibited SVZ-derived NSC proliferation. Regarding the antiproliferative effect of NO, we found that @entity45337 caused the impairment of signaling through the ERK/MAPK pathway, which may be related to increased nitration of the EGF receptor in NSC. Using @entity42043 nitration was prevented, maintaining ERK signaling, rescuing NSC proliferation. We show that NO from inflammatory origin leads to a decreased function of the EGF receptor, which compromised proliferation of NSC. We also demonstrated that NO-mediated nitration of the EGF receptor caused a decrease in its phosphorylation, thus preventing regular proliferation signaling through the ERK/MAPK pathway.

XXXX from inflammatory origin impairs neural stem cell proliferation by inhibiting epidermal growth factor receptor signaling.

@entity45337 :: ('MESH:C093285', 'Chemical') :: ['NOC-18'] @entity1568 :: ('MESH:D009584', 'Chemical') :: ['nitrogen'] @entity42043 :: ('MESH:C097284', 'Chemical') :: ['MnTBAP'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity29101 :: ('CHEBI:33258', 'Chemical') :: ['ONOO(-)'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity6710 :: ('MESH:D030421', 'Chemical') :: ['peroxynitrite']
@entity842 :: (MESH:D009569,Chemical) :: ['Nitric oxide']
