@entity729 is associated with @entity65 and mortality. @entity2265 is an important contributor to @entity729 . Our previous studies demonstrated that elevated @entity1391 induces @entity730 of @entity3960 ( @entity3960 ) in vitro. Inhibition of @entity1391 transport by @entity4541 blocked @entity1391 -induced @entity730 , implicating @entity1372 -dependent @entity1391 cotransporters in this process. In the present study, we have investigated the role of the type III @entity1372 -dependent @entity1391 cotransporter, @entity10914 , in @entity3960 @entity730 in vitro. @entity1 @entity3960 stably expressing @entity10914 small interfering double-stranded RNA ( @entity3960 -iRNA) were established using a retroviral system. @entity3960 -iRNA had decreased @entity10914 mRNA and protein levels and @entity1372 -dependent @entity1391 transport activity compared with the control transduced cells ( @entity3960 -CT) (2.9 versus 9.78 nmol/mg protein per 30 minutes, respectively). Furthermore, @entity1391 -induced @entity3960 @entity730 was significantly inhibited in @entity3960 -iRNA compared with @entity3960 -CT at all time points examined. Overexpression of @entity10914 restored @entity1391 uptake and @entity1391 -induced @entity730 in @entity10914 deficient cells. Mechanistically, although @entity10914 -mediated @entity3960 @entity730 was not associated with apoptosis or cell-derived vesicles, inhibition of @entity1391 uptake in @entity10914 knockdown cells blocked the induction of the osteogenic markers @entity8087 and @entity1096 . Our results indicate that @entity1391 uptake through @entity10914 is essential for @entity3960 @entity730 and phenotypic modulation in response to elevated @entity1391 .

Role of the @entity1372 -dependent @entity1391 cotransporter, @entity10914 , in vascular smooth muscle cell XXXX .

@entity1 :: ('9606', 'Species') :: ['Human'] @entity8087 :: ('860', 'Gene') :: ['Cbfa-1'] @entity10914 :: ('5449', 'Gene') :: ['Pit-1'] @entity4541 :: ('MESH:D017245', 'Chemical') :: ['phosphonoformic acid'] @entity1372 :: ('MESH:D012964', 'Chemical') :: ['sodium'] @entity730 :: ('MESH:D002114', 'Disease') :: ['calcification'] @entity2265 :: ('MESH:D054559', 'Disease') :: ['Hyperphosphatemia'] @entity1391 :: ('CHEBI:26020', 'Chemical') :: ['phosphate'] @entity3960 :: ('MESH:D018235', 'Disease') :: ['smooth muscle cells', 'SMC'] @entity1096 :: ('6696', 'Gene') :: ['osteopontin'] @entity729 :: ('MESH:D061205', 'Disease') :: ['vascular calcification', 'Vascular calcification'] @entity65 :: ('MESH:D002318', 'Disease') :: ['cardiovascular morbidity']
@entity730 :: (MESH:D002114,Disease) :: ['calcification']
