@entity1 with @entity0 in a distinct stage of disease can benefit from @entity34 ( @entity34 ) with autologous stem cell support; however, a significant number of these @entity1 relapse despite this intensive treatment. This study investigates the persistence of @entity5 on the single-cell level. A total of 194 data sets consisting of bone marrow and blood samples obtained prior to and after @entity34 and of aliquots of apheresis products were searched with immunocytochemistry and reverse transcriptase polymerase chain reaction (RT-PCR) for disseminated @entity5 cells. Presence of @entity5 cells in the marrow is frequent prior to and after @entity34 , but @entity34 reduces the amount of malignant cells in marrow significantly. In contrast, there was no effect on the number of circulating @entity5 cells. Reinfusion of contaminated apheresis products was surprisingly associated with a low number of malignant cells in bone marrow after @entity34 and vice versa. The impact of disseminated @entity5 cells in bone marrow, apheresis, and peripheral blood on disease-free survival after @entity34 could be investigated in a total of 165 samples. Surprisingly, neither the presence of @entity5 cells in marrow or blood nor in apheresis was associated with a bad prognosis in Kaplan-Meyer survival analysis. These results suggest that apheresis products and bone marrow should be regarded as different biological compartments for @entity5 cells. It can be concluded that complete elimination of disseminated @entity5 cells by @entity34 is not always possible. The theory of reinduction of metastatic @entity0 by accidentally reinfused contaminants is not supported by this study so far. However, further research is necessary to identify distinct cell populations with the potentially capacity to metastasize.

Disseminated @entity0 cells prior to and after XXXX .

@entity1 :: ('9606', 'Species') :: ['patients', 'Women'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'epithelial cancer', 'malignancy'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity34 :: ('MESH:D016609', 'Disease') :: ['high-dose therapy', 'HDT']
@entity34 :: (MESH:D016609,Disease) :: ['high-dose therapy']
