@entity1 and nonhuman primates have multiple DR B1 and non-B1 alleles. However, the role of mismatched DR non-B1 alleles in primary alloimmune responses is not well understood. Macaques, which share close DNA homologies with @entity1 MHC genes and have a high number of beta-chain genes in the DR subregion, are preeminent preclinical models for immunologic studies of transplant tolerance and immunosuppression. In this study, we examined the effect of allogeneic MHC Class II DRB mismatches in Th1- and Th2-like cytokine responses elicited in one-way MLR cultures in @entity2608 . An ELISPOT method was used to estimate cytokine secretion at the single cell level. Molecular typing for @entity49493 and DR non-B1 alleles was performed by a moderate-high resolution PCR-SSP method using a panel of 55 primer pairs covering 74 DRB alleles and clusters. Of 35 unrelated combinations, 66% had multiple (> or = 2) allelic MM at @entity49493 and DR non-B1 with no significant correlation between numbers of @entity49493 and DR non-B1 mismatches. Pairs with 1 or 0 MM were assigned to a mono/null MM group to obtain sufficient numbers for statistical analysis. The pairs differing by multiple vs. mono/null @entity49493 MM showed no significant difference in cytokine prevalence (P = 0.69). In contrast, high @entity64330 / @entity51342 SFC ratios were noted in pairs with multiple vs. mono/null DR non-B1 MM (p = 0.0009). @entity64330 / @entity57330 spot forming cell (SFC) ratios were consistent with @entity64330 / @entity51342 SFC ratios (r = 0.98). Multiple DR non-B1 mismatches showed a trend towards higher MLR proliferative responses, although the stimulation index did not reflect the dominant cytokine response. These observations suggest a bias towards Th1-like cytokine production under allostimulation with multiple DR non-B1 gene products. Further study of the primary structure of DR non-B1 determinants may be helpful in understanding the fine molecular mechanisms governing the regulation of cytokine profiles during allostimulation in primates.

DR non-B1 mismatches influence allogeneic MLR-induced TH1- or TH2-like cytokine responses in XXXX .

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity64330 :: ('574282(Tax:9544)', 'Gene') :: ['IFN-gamma'] @entity57330 :: ('694931(Tax:9544)', 'Gene') :: ['IL-10'] @entity2608 :: ('9544', 'Species') :: ['rhesus macaques'] @entity49493 :: ('100428435', 'Gene') :: ['DRB1'] @entity51342 :: ('574281(Tax:9544)', 'Gene') :: ['IL-4', 'IL4']
@entity2608 :: (9544,Species) :: ['rhesus monkeys']
