Despite many efforts to alter the relentlessly aggressive progression of @entity5 of neural origin, individuals bearing these @entity5 exhibit poor prognosis for long-term survival. In an attempt to find an effective treatment, we examined the efficacy of the non-steroidal anti-inflammatory drug, @entity8726 , to suppress the growth of @entity5 cell lines derived from @entity2946 and @entity463 multiforme. Results from cell proliferation assays have revealed that @entity8726 effectively inhibits the growth of various @entity5 cells in a dose-dependent manner and causes a noticeable change in the progression of cells through cell cycle stages. Treatment of @entity5 cells with @entity8726 reduced the number of cells in G1 and G2, and significantly increased their numbers in S phase, suggesting that, @entity8726 accelerates G1/S entry, and/or delays cell exit from S to G2/M stages. Results from RNase protection assay and Western blot analysis showed that while treatment of cells with @entity8726 causes a minor change in the RNA level of different cyclins, there is a significant decrease in the level of cyclin B protein upon @entity8726 treatment. Examination of @entity5 suppressors by RNase protection technique showed a subtle increase in the levels of several @entity5 suppressors upon @entity8726 treatment. Interestingly, at the protein level, @entity876 @entity5 suppressor was substantially increased upon @entity8726 treatment, yet the level of @entity3544 , a downstream target for @entity876 remained unchanged. Curiously, treatment of the cells with @entity8726 enhanced the level of @entity2247 expression. Results from co-immunoprecipitation showed association of @entity2247 with @entity876 in @entity5 cells. These observations suggest that the interaction of @entity2247 with @entity876 may cause @entity3544 -independent suppression of @entity5 cell growth upon @entity8726 treatment.

Inhibition of human brain tumor cell growth by the anti-inflammatory drug, XXXX .

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity3544 :: ('644914', 'Gene') :: ['p21'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity2946 :: ('MESH:D008527', 'Disease') :: ['medulloblastoma'] @entity2247 :: ('4513', 'Gene') :: ['COX-2'] @entity463 :: ('MESH:D005909', 'Disease') :: ['glioblastoma'] @entity8726 :: ('MESH:D005480', 'Chemical') :: ['flurbiprofen']
@entity8726 :: (MESH:D005480,Chemical) :: ['flurbiprofen']
