UNASSIGNED: MiRNAs play crucial roles in development of @entity5 . However, the underlying mechanisms of miRNAs in @entity421 ( @entity421 ) are poorly understood. This study is aimed to investigate the clinical importance of miR-125b and @entity40734 expression in @entity421 tissue samples using real-time PCR.Total RNA was purified from samples and noncancerous bone tissues and then real-time PCR was used to evaluate the expression rate of microRNAs. Furthermore, the association of miR-125b and @entity40734 level with pathological factors and prognosis were evaluated. Our results suggested that miR-125b expression was significantly downregulated in @entity421 bone tissue compared with noncancerous bone tissues. On the other hand, @entity40734 expression was significantly upregulated in @entity421 bone tissue than noncancerous bone tissues.Our findings suggested that a lower expression level of miR-125b was linked to advanced clinical stage (P   =   0.005), @entity3 (P   =   0.004), and a large size of @entity5 (P   =   0.023). In addition, a higher expression level of @entity40734 was related to advanced @entity5 ( @entity5 ) stage (P   =   0.002), @entity3 (P   =   0.001), and a large size of @entity5 (P   =   0.038). The lower expression and higher expression of mentioned microRNAs was significantly linked to shorter overall survival according to the Kaplan-Meier survival and log-rank analysis (log-rank test P   =   0.026; P   =   0.017).Moreover, lower expression of miR-125b (P   =   0.001), a decreased expression level of @entity40734 (P   =   0.004), @entity5 stage (P   =   0. 0.004; P   =   0.003), @entity3 (P   =   0.027; P   =   0.001), and a large @entity5 size (P   =   0.006; P   =   0.025) were independent prognostic markers of overall survival of @entity1 according to the multivariate Cox proportional hazards model. In conclusion, our results indicated that downregulation of miR-125b and @entity40734 was associated with progression of @entity421 and both of them may act as @entity5 suppressor in @entity421 .

Upregulation of XXXX and downregulation of miR-125b act as potential predictor biomarkers in progression, @entity3 , and poor prognosis of @entity421 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'TNM', 'tumor, node, and metastasis', 'cancer'] @entity421 :: ('MESH:D012516', 'Disease') :: ['OS', 'osteosarcoma'] @entity40734 :: ('100126297', 'Gene') :: ['MiR-300', 'miR-300'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis']
@entity40734 :: (100126297,Gene) :: ['miR-300']
