AIMS: To investigate the @entity137 and bioactivity of several pulpotomy materials: @entity4800 (Septodont, Saint- Maur-des-Fosses, France) MTA (Angelus, Londrina, PR, Brazil), Theracal LC (Bisco Inc., Schamburg, IL, USA) and IRM (Dentsply DeTrey GmbH, Konstanz, Germany), after contact with stem cells isolated from @entity1 exfoliated deciduous teeth (SHEDs). METHODOLOGY: SHEDs were cultured in the presence of the eluates of various pulpotomy materials for 24, 48 and 72h. Cell viability was determined by mitochondrial dehydrogenase enzymatic (MTT) assay. Apoptosis and changes in cell phenotype were evaluated by flow cytometry. Also, an in vitro scratch wound healing assay was used to determine their effects on cell migration. To assess cell morphology and attachment to the different pulpotomy materials, SHEDs were directly seeded onto the material surfaces and analysed by @entity4653 ( @entity4653 ). Finally, the deposition of a calcified matrix in presence of these materials was verified by @entity622 staining. Statistical analysis was performed with analysis of variance and Bonferroni or Tukey post-test (a = 0.05). RESULTS: Cell viability in the presence of @entity4800 eluates was significantly higher to that obtained using complete medium alone (control) (p<0.01), and was also significantly higher than using MTA Angelus from 48h of incubation (p<0.01). However, Theracal LC and IRM were associated with low rates of cell viability (p<0.001). Similar results were obtained in an apoptosis assay. In addition, SHEDs maintained their mesenchymal phenotype in all conditions although their capacity to migrate was higher in the presence of @entity4800 . @entity4653 studies revealed a suitable proliferation rate, cell spreading and attachment, especially when using @entity4800 and MTA Angelus disks. Finally, @entity4800 eluates significantly induced calcified matrix deposition from 7 days of culture (p<0.01). CONCLUSIONS: @entity4800 exhibited better cytocompatibility and bioactivity than MTA Angelus, Theracal LC and IRM. This article is protected by copyright. All rights reserved.

@entity137 and bioactivity of various pulpotomy materials on stem cells from XXXX exfoliated primary teeth.

@entity1 :: ('9606', 'Species') :: ['human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity622 :: ('MESH:C010078', 'Chemical') :: ['alizarin red'] @entity4800 :: ('MESH:C506393', 'Chemical') :: ['Biodentine'] @entity4653 :: ('MESH:D004401', 'Disease') :: ['SEM', 'scanning electron microscopy']
@entity1 :: (9606,Species) :: ['human']
