OBJECTIVE: To establish the methods to get high activity, high purity, and adequate Schwann cells (SCs), and to provide sufficient seed cells for the peripheral nerve repair. METHODS: Six 5-day-old, male or female, @entity35 were selected and the sciatic nerve (control group) and dorsal root ganglion (DRG) (experimental group) were harvested. Then the sciatic nerves and DRG were digested by co-enzyme and dispersed by medium containing serum to isolate SCs. Freshly isolated SCs from @entity35 were cultured, purified and subcultured. The 1st generation of SCs were chosen to draw the growth curve of SCs by the counting method and to detect the proliferation of SCs by @entity98 assay at 8 days of culture, the purity of SCs by immunocytochemistry of anti-S-100 and the @entity2136 ( @entity2136 ) concentration by ELISA. RESULTS: A total of 36-43 DRGs could be obtained in each @entity35 . The number of obtained single SC in experimental group [(7.5 +/- 0.6) x 10(6)] was significantly higher than that in control group [(3.5 +/- 0.4) x 10(6)] (t = 13.175, P=0.000). SCs reached logarithm proliferation phase at 3 days. With time, the cell number and the proliferation absorbance (A) value of 2 groups all showed upward trend. The number and A value of experimental group were significantly higher than those of control group (P < 0.05). The SCs purity of experimental group (92.08% +/- 3.45%) was significantly higher than that of control group (77.50% +/- 3.57%) (t = 6.689, P = 0.001). The concentrations of @entity2136 at 3 days and 5 days in experimental group were significantly higher than those of control group (P < 0.05). CONCLUSION: The sufficient amount, high purity, and viability of SCs from DRGs can meet the needs of studies on peripheral nerve repairment.

[Study of isolation and purification of primary Schwann cells from different parts of nerve tissue in XXXX ].

@entity35 :: ('10116', 'Species') :: ['rat', 'Sprague Dawley rats', 'rats'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity2136 :: ('24225(Tax:10116)', 'Gene') :: ['BDNF', 'brain-derived neurotrophic factor']
@entity35 :: (10116,Species) :: ['rats']
