Peripheral CD8(+) T cells are defective in both @entity21692 and @entity15593 knock-out (KO) @entity19 ; however, whether @entity21692 / @entity15593 deficiency has a similar effect on CD8 single-positive (SP) thymocytes remains unclear. In this study, we investigated whether the absence of @entity21692 transpresentation in @entity15593 KO @entity19 results in a defect in thymic CD8 single positive (SP) TCR(hi) thymocytes. Comparison of CD8SP TCR(hi) thymocytes from @entity15593 KO @entity19 with their @entity164 ( @entity164 ) counterparts by flow cytometry showed a significant reduction in the percentage of @entity26490 (-) CD8SP TCR(hi) thymocytes, which represent thymic premigrants. In addition, analysis of in vivo @entity1603 ( @entity1603 ) incorporation demonstrated that premigrant expansion of CD8SP TCR(hi) thymocytes was reduced in @entity15593 KO @entity19 . The presence of @entity21692 transpresentation-dependent expansion in CD8SP TCR(hi) thymocytes was assessed by culturing total thymocytes in @entity15593 -Fc fusion protein-pre-bound plates that were pre-incubated with @entity21692 to mimic @entity21692 transpresentation in vitro. The results demonstrated that CD8SP thymocytes selectively outgrew other thymic subsets. The contribution of the newly divided CD8SP thymocytes to the peripheral CD8(+) T cell pool was examined using double labeling with intrathymically injected @entity2823 and intravenously injected @entity1603 . A marked decrease in FITC(+) @entity1603 (+) CD8(+) T cells was observed in the @entity15593 KO lymph nodes. Through these experiments, we identified an @entity21692 transpresentation-dependent proliferation process selective for the mature CD8SP premigrant subpopulation. Importantly, this process may contribute to the maintenance of the normal peripheral CD8(+) T cell pool.

Selective reduction of post-selection CD8 thymocyte proliferation in XXXX deficient @entity19 .

@entity1603 :: ('MESH:D001973', 'Chemical') :: ['BrdU', '5-bromo-2-deoxyuridine'] @entity26490 :: ('12515(Tax:10090)', 'Gene') :: ['CD69'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity15593 :: ('16169(Tax:10090)', 'Gene') :: ['IL-15Ra'] @entity21692 :: ('16168(Tax:10090)', 'Gene') :: ['IL-15'] @entity2823 :: ('MESH:D016650', 'Chemical') :: ['FITC'] @entity164 :: ('MESH:D017827', 'Disease') :: ['WT', 'wild type']
@entity15593 :: (16169(Tax:10090),Gene) :: ['IL-15Ra']
