We tested the hypothesis that postnatal alterations in @entity8586 ( @entity6140 ) receptors are associated with developmental changes in enzyme secretory response. We used simultaneous measurements of @entity6140 receptor binding and amylase release in pancreatic acini isolated from @entity35 pups at various ages (1, 2, 5, 6, 18, and 36 days). @entity6140 receptor binding was analyzed using the LIGAND program. The affinity of the high-affinity state increased postnatally at 18 and 36 days (p < 0.05); the capacity of the high-affinity state also increased at 2 days (p < 0.05), then declined sequentially up to 36 days. The affinity of the low-affinity state increased postnatally reaching statistical significance at 5 days; the capacity of the low-affinity state increased twofold at 2 days, reaching statistical significance at 5 days (p < 0.05); this was followed by a slight decrease at 36 days. At 1 day postnatally a small amylase response occurred (p < 0.05), but no dose-dependent response was observed. A significant @entity6140 dose-dependent secretory response occurred at all other ages. Maximal amylase release was highest at 18 days (p < 0.05). @entity6140 doses required to stimulate maximal amylase release were 20, 2, 1, 0.2 and 0.4 nM at 2, 5, 6, 18, and 36 days, respectively. The receptor occupancy rates for high- and low-affinity states decreased sequentially between 2 and 18 days of age, when maximal amylase release occurred. These data suggest that more spare receptors become available with increasing postnatal age. We conclude that postnatal alterations of both high- and low-affinity states of @entity6140 receptors in pancreatic acini are associated with developmental changes in enzyme secretory response to @entity6140 . An increase in the affinity of high-affinity state and the capacity of the low-affinity state may enhance acinar sensitivity to @entity6140 .

Postnatal development of the @entity35 exocrine pancreas. I. Alterations in high- and low-affinity XXXX receptors and enzyme secretion.

@entity35 :: ('10116', 'Species') :: ['rat'] @entity6140 :: ('25298(Tax:10116)', 'Gene') :: ['CCK'] @entity8586 :: ('MESH:D002766', 'Chemical') :: ['cholecystokinin']
@entity8586 :: (MESH:D002766,Chemical) :: ['cholecystokinin']
