UNASSIGNED: Milk has long been known and used to promote sleep. The sleep-promoting effect of milk has been attributed to its psychological associations (i.e., the memory of a mother giving milk at bedtime) and its rich store of sleep-promoting constituents (e.g., @entity6635 ). Studies have shown that milk harvested at night (Night milk) contains exceptionally high amounts of @entity6635 and @entity2493 . In the present study, we evaluated the psychopharmacological properties of Night milk, particularly its probable sleep-promoting/enhancing, and anxiolytic effects. Night milk was orally administered to ICR @entity19 at various concentrations (100, 200, or 300   mg/kg). An hour after administration, assessment of its sedative (open-field and rotarod tests) and sedative sleep-potentiating effects ( @entity4485 -induced sleeping test) was conducted. For comparison, the effects of Day milk (daytime milking) were also assessed. In addition, the effects of Night milk on @entity146 (elevated plus maze [EPM] test) and electroencephalographic (EEG) waves were evaluated. Night milk-treated animals exhibited @entity393 (open-field test) and @entity73 (rotarod test). Furthermore, Night milk shortened the sleep onset and prolonged the sleep duration induced by @entity4485 . These effects were comparable to that of @entity3175 . In addition, Night milk significantly increased the percentage of time spent and entries into the open arms of the EPM, indicating that it also has anxiolytic effects. No significant changes in EEG waves were observed. Altogether, these findings suggest that Night milk is a promising natural aid for sleep- and @entity148 .

Milk Collected at Night Induces Sedative and Anxiolytic-Like Effects and Augments @entity4485 -Induced Sleeping Behavior in XXXX .

@entity4485 :: ('MESH:D010424', 'Chemical') :: ['pentobarbital', 'pentobarbital sodium'] @entity3175 :: ('MESH:D003975', 'Chemical') :: ['diazepam'] @entity146 :: ('MESH:D001523', 'Disease') :: ['anxiety behavior'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity6635 :: ('D014364', 'Chemical') :: ['tryptophan'] @entity73 :: ('MESH:D003072', 'Disease') :: ['impaired motor balance and coordination'] @entity2493 :: ('MESH:D008550', 'Chemical') :: ['melatonin'] @entity148 :: ('MESH:D001008', 'Disease') :: ['anxiety-related disturbances'] @entity393 :: ('MESH:D034381', 'Disease') :: ['decreased spontaneous locomotion']
@entity19 :: (10090,Species) :: ['Mice']
