High mortality incidence after serious systemic @entity130 is believed to be linked to significant increases in cerebral permeability, ultimately leading to irreversible blood-brain barrier (BBB) breakdown. The aim of this study was to investigate whether disruption of microvascular integrity in a @entity35 @entity130 model is associated with early matrix metalloproteinase (MMP) expression. A total of 35 @entity35 were studied in @entity130 and control groups, each group containing two subgroups, one for @entity2657 and Evans blue analysis and another for MMP mRNA analysis. Thermally injured animals were anesthetized and submerged vertically in 85 degrees C water to the neck for 6 seconds producing a third degree burn affecting 70% of the total body surface area. BBB integrity was determined by measuring amount of Evans blue after 7 hours of injury with a spectrophotometer. @entity2657 was detected by calculating water content. Brain mRNA levels were determined with real-time PCR 3 and 7 hours post-injury. Brain water content was significantly increased after peripheral injury at hour 7. @entity5934 was also significantly increased at the same time, suggesting an impaired BBB function after injury. Expressions of @entity11017 and @entity6929 mRNA in brain were increased as early as 3 hours after injury and remained at hour 7. Our study demonstrated a significant increase in cerebral permeability that occurs after serious systemic @entity130 . The underlying mechanisms could be related to early expression of MMPs.

Peripheral thermal injury causes early blood-brain barrier dysfunction and matrix metalloproteinase expression in XXXX .

@entity2657 :: ('MESH:D001929', 'Disease') :: ['Brain edema', 'brain edema'] @entity6929 :: ('81687(Tax:10116)', 'Gene') :: ['MMP-9'] @entity5934 :: ('MESH:C536380', 'Disease') :: ['Evans blue leakage'] @entity35 :: ('10116', 'Species') :: ['rat', 'Sprague-Dawley rats'] @entity11017 :: ('81686(Tax:10116)', 'Gene') :: ['MMP-2'] @entity130 :: ('MESH:D014947', 'Disease') :: ['thermal injury']
@entity35 :: (10116,Species) :: ['rat']
