PURPOSE: Technetium-99  m (Tc-99  m)-labelled microparticles, functionalized with fucoidan to present a high affinity for @entity8729 , or [(99m)Tc] MP-fucoidan, were developed as a novel SPECT radiotracer for @entity1028 ( @entity1028 ). As a prerequisite step forwards a clinical trial, the biodistribution and dosimetry of these [(99m)Tc] MP-fucoidan microparticles were performed in @entity35 in order to estimate the absorbed and effective dose in @entity1 . PROCEDURES: Microparticles with a maximum hydrodynamic diameter of 4   m were obtained by crosslinking polysaccharides dextran and pullulan. They were functionalized with fucoidan then radiolabelled with Tc-99  m. A mean labelling efficiency of 92        1  % was measured. [(99m)Tc] MP-fucoidan (43        2  MBq) was injected to 24 @entity35 via the penis vein. @entity35 were euthanized at 30, 60, 120 and 240  min after injection (4 @entity35 at each time point). Samples of each organ, as well as the injected microparticle suspensions, were aliquoted for counting. Four animals were sacrificed for blood clearance studies and four were sacrificed for image analysis and quantification of the cortical, medullary, papillary kidney, and pelvis uptake. A compartmental model was realised using SAAM II and organ data were fitted. The area under the curve was then used to compute the residence times in each @entity35 organs and converted to @entity1 residence time values. Absorbed and effective @entity1 doses in organs were estimated using (1) the OLINDA/EXM 1.1 software with the hermaphroditic mathematical phantoms and (2) the OEDIPE software associated to the MCNPX Monte Carlo code and the ICRP reference computational male and female phantoms, using the updated tissue weighting factors in the ICRP Publication 103. RESULTS: The highest @entity1 residence times were found in the liver, kidneys, and urinary bladder wall. The largest doses were found in the kidneys and then in the urinary bladder wall and liver. The @entity1 effective doses were 6.06   Sv/MBq for the hermaphroditic mathematical phantom and 5.95   Sv/MBq for the ICRP adult reference computational phantom. CONCLUSIONS: Animal-based @entity1 dose estimates support a future first-in- @entity1 testing of [(99m)Tc] MP-fucoidan following IV injection.

Evaluation of Functionalized Polysaccharide Microparticles Dosimetry for SPECT Imaging Based on Biodistribution Data of XXXX .

@entity35 :: ('10116', 'Species') :: ['rat', 'Rats', 'rats'] @entity1 :: ('9606', 'Species') :: ['humans', 'human'] @entity8729 :: ('6403', 'Gene') :: ['P-Selectin'] @entity1028 :: ('MESH:D017544', 'Disease') :: ['abdominal aortic aneurysm', 'AAA']
@entity35 :: (10116,Species) :: ['Rats']
