BACKGROUND/AIMS: Endothelial cell injury and subsequent death play an essential role in the pathogenesis of @entity731 . Autophagy of endothelial cells has a protective role against development of @entity731 , whereas the molecular regulation of endothelial cell autophagy is unclear. MicroRNA-30 ( @entity21445 -30) is a known autophagy suppressor in some biological processes, while it is unknown whether this regulatory axis may be similarly involved in the development of @entity731 . Here, we aimed to answer these questions in the current study. METHODS: We examined the levels of endothelial cell autophagy in @entity6970 (-/-) @entity19 suppled with high-fat diet (HFD), a @entity19 model for @entity731 (simplified as HFD @entity19 ). We analyzed the levels of @entity34734 ( @entity34734 , or Beclin-1) and the levels of @entity21445 -30 in the purified @entity5231 + endothelial cells from @entity19 aorta. Prediction of the binding between @entity21445 -30 and 3'-UTR of @entity34734 mRNA was performed by bioinformatics analyses and confirmed by a dual luciferase reporter assay. The effects of @entity21445 -30 were further analyzed in an in vitro model using oxidized low-density lipoprotein (ox-LDL)-treated @entity1 aortic endothelial cells (HAECs). RESULTS: HFD @entity19 developed @entity731 in 12 weeks, while the control @entity6970 (-/-) @entity19 that had received normal diet (simplified as NOR @entity19 ) did not. Compared to NOR @entity19 , HFD @entity19 had significantly lower levels of endothelial cell autophagy, resulting from decreases in @entity34734 protein, but not mRNA. The decreases in @entity34734 in endothelial cells were due to HFD-induced increases in @entity21445 -30, which suppressed the translation of @entity34734 mRNA via 3'-UTR binding. These in vivo findings were reproduced in vitro on ox-LDL-treated HAECs. CONCLUSION: Upregulation of @entity21445 -30 by HFD may impair the protective effects of endothelial cell autophagy against development of @entity731 through suppressing protein translation of @entity34734 .

Endothelial Cell Autophagy in @entity731 is Regulated by XXXX -30-Mediated Translational Control of @entity34734 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity6970 :: ('11816(Tax:10090)', 'Gene') :: ['ApoE'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity21445 :: ('751557(Tax:10090)', 'Gene') :: ['miR'] @entity5231 :: ('18613(Tax:10090)', 'Gene') :: ['CD31'] @entity731 :: ('MESH:D050197', 'Disease') :: ['atherosclerosis'] @entity34734 :: ('56208(Tax:10090)', 'Gene') :: ['autophagy-associated protein 6', 'ATG6']
@entity21445 :: (751557(Tax:10090),Gene) :: ['miR']
