INTRODUCTION: Recently a mini-spectrometer with a handheld probe quantifying @entity2518 ( @entity2518 ) based fluorescence intensity of @entity1313 was developped by Kim and co-workers to improve fluorescence-guided neurosurgery. OBJECTIVE: To evaluate if this new tool is capable to discriminate nuances of fluorescence intensity of strongly fluorescing @entity5 ( @entity463 ( @entity463 ) and @entity1653 ( @entity1653 )). To study different modes of measurement (touch/no-touch). To determine @entity13880 ( @entity13880 ) concentration in @entity5 tissue as compared to a laboratory spectrometer. MATERIAL AND METHODS: The @entity5 tissue was resected from @entity1 operated in the neurosurgical department of University Hospital Duesseldorf, Germany between 01/2014 and 06/2014. Two spectrometers, one custom-built with a handheld probe ("mini-spectrometer") and one commercial laboratory spectrometer were employed. After calibration they were used to detect and compare fluorescence intensity of @entity1 @entity1313 samples ex vivo under standardized conditions. The mini-spectrometer was tested at different distances to the @entity5 . @entity13880 concentrations of @entity5 lysates were determined by both spectrometers. RESULTS: In total n=11 @entity5 (5 @entity1653 and 6 @entity463 ) resulting in 17 @entity5 biopsies were studied. All @entity463 showed significant higher fluorescence intensity as compared to @entity1653 ( @entity69354 , p=0.001). The fluorescence signal was inversely proportional to the square of the distance ( @entity463 : R(2)=0.226; F=4.683; p<0.5; @entity1653 : R(2)=0255; F=8.042; p<0.01). The mini-spectrometer recorded fluorescence signals up to 2 millimeters ("no-touch"). Determination of @entity13880 concentration in @entity5 by the mini-spectrometer did not differ from a laboratory spectrometer. CONCLUSION: The mini-spectrometer was a very sensitive tool for detection of @entity2518 based fluorescence of @entity1 @entity1313 . Fluorescence intensity of @entity463 and @entity1653 were significantly different. A no-touch mode of measurement was possible. @entity13880 concentration in @entity5 tissue could be determined as precisely as with a laboratory spectrometer. In future clinical trials the practicability of using such a tool in vivo has to be further evaluated.

Minispectrometer with handheld probe for @entity2518 based fluorescence-guided surgery of XXXX : preliminary study for clinical applications.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity69354 :: ('962026(Tax:155864)', 'Gene') :: ['Z=-3.123'] @entity1313 :: ('MESH:D001932', 'Disease') :: ['brain tumor', 'brain tumors'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity2518 :: ('CHEBI:17549', 'Chemical') :: ['5-ALA', '5-aminolevulinic acid'] @entity1653 :: ('MESH:D008579', 'Disease') :: ['meningioma', 'MM', 'meningiomas'] @entity13880 :: ('MESH:C028025', 'Chemical') :: ['protoporphyrin IX', 'PPIX'] @entity463 :: ('MESH:D005909', 'Disease') :: ['glioblastomas', 'GBM', 'glioblastoma']
@entity1313 :: (MESH:D001932,Disease) :: ['brain tumors']
