UNASSIGNED: The need to have a better and safer culture condition for expansion of @entity1 mesenchymal stem cells (MSCs) is crucial particularly to prevent @entity281 and immune rejection. This is normally associated with the use of animal-based serum in the culture media for cell expansion. The aim of this study is to investigate alternative culture conditions which may provide better and safer environment for cell growth. In the present study, @entity1 adipose-derived stem cells (ASCs) at passage 3 were subjected to treatment in 4 conditions: (1) 21  % @entity3612 with @entity2241 ( @entity2241 ), (2) 21  % @entity3612 without @entity2241 , (3) 2  % @entity3612 with @entity2241 and (4) 2  % @entity3612 without @entity2241 followed by subsequent analysis of their phenotype, viability and functionality. We observed that ASCs cultured in all conditions present no significant phenotypic changes. It was found that ASCs cultured in 2  % @entity3612 without serum showed an increase in viability and growth to a certain extent when compared to those cultured in 21  % @entity3612 without serum. However, ASCs cultured in 2  % @entity3612 without serum displayed a relatively low adipogenic and osteogenic potential. On the other hand, interestingly, there was a positive enhancement in chondrogenic differentiation of ASCs cultured in 21  % @entity3612 without serum. Our findings suggest that different culture conditions may be suitable for different indications. In summary, ASCs cultured in serum-free condition can still survive, proliferate and undergo subsequent adipogenic, osteogenic and chondrogenic differentiation. Therefore, @entity2241 is feasible to be excluded for culture of ASCs, which avoids clinical complications.

The effects of hypoxia and serum-free conditions on the stemness properties of XXXX adipose-derived stem cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity3612 :: ('D013481', 'Chemical') :: ['O2'] @entity2241 :: ('MESH:D005315', 'Disease') :: ['FBS', 'fetal bovine serum']
@entity1 :: (9606,Species) :: ['human']
