@entity2135 ( @entity2135 ) is induced after @entity63 ( @entity63 ) @entity1545 , and therapeutic studies suggest that @entity2135 may restrict delayed neuronal and glial cell loss. We have used a well-characterised @entity35 model of @entity63 to extend our understanding of the modes of action of the IGF system after injury. The induction of the IGF system by injury was examined by in situ hybridization, immunohistochemistry, Northern blot analysis, RNase protection assay and reverse transcriptase-polymerase chain reaction (RT-PCR). @entity2135 accumulated in blood vessels of the damaged hemisphere within 5 h after a severe injury. By 3 days, @entity2135 mRNA was expressed by reactive microglia in regions of delayed @entity528 , and immunoreactive @entity2135 was associated with these microglia and reactive astrocytes juxtaposed to surviving neurones surrounding the @entity1188 . Total @entity7814 mRNA was unchanged by the injury. @entity7815 mRNA was strongly induced in reactive astrocytes throughout the injured hemisphere, and @entity9929 and @entity10778 mRNA were moderately induced in reactive microglia and neurones of the injured hippocampus, respectively. @entity2619 mRNA was induced in the damaged hemisphere by 3 days and increased protein was seen on the choroid plexus, ependyma and reactive glia. In contrast, insulin II was not induced. These results indicate cell type-specific expression for @entity2135 , @entity7815 ,3,5 and 6 after injury. Our findings suggest that the @entity2135 produced by microglia after injury is transferred to perineuronal reactive astrocytes expressing @entity7815 . Thus, modulation of @entity2135 action by @entity7815 might represent a key mechanism that restricts neuronal cell loss following @entity63 @entity1545 .

Co-ordinated and cellular specific induction of the components of the IGF/IGFBP axis in the XXXX brain following @entity63 .

@entity1545 :: ('MESH:D020214', 'Disease') :: ['brain injury'] @entity7815 :: ('25662(Tax:10116)', 'Gene') :: ['IGFBP-2'] @entity63 :: ('MESH:D007511', 'Disease') :: ['HI', 'hypoxic-ischemic', 'HI injury'] @entity10778 :: ('25285(Tax:10116)', 'Gene') :: ['IGFBP-5'] @entity2135 :: ('24482(Tax:10116)', 'Gene') :: ['IGF-1', 'Insulin-like growth factor 1'] @entity1188 :: ('MESH:D007238', 'Disease') :: ['infarct'] @entity2619 :: ('25641(Tax:10116)', 'Gene') :: ['IGFBP-6'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity9929 :: ('24484(Tax:10116)', 'Gene') :: ['IGFBP-3'] @entity528 :: ('MESH:D009410', 'Disease') :: ['neuronal death'] @entity7814 :: ('25718(Tax:10116)', 'Gene') :: ['IGF-1 receptor']
@entity35 :: (10116,Species) :: ['rat']
