MDA-MB-231, an HLA-A2(+), @entity1722 /neu(+) allogeneic @entity0 cell line genetically modified to express the costimulatory molecule @entity7938 (B7-1), was used to vaccinate 30 @entity1 with previously treated @entity0 . Expression of @entity7938 conferred the ability to deliver a costimulatory signal and thereby improved the antigen presentation capability of the @entity5 cells to @entity1 T cells in vitro. @entity1 were vaccinated with 10(7) or 10(8) irradiated gene-modified @entity5 cells with @entity2841 ( @entity2841 ) or BCG, three times at 2-week intervals and then monthly until progressive disease developed. @entity2841 -related flulike symptoms and minor injection site reactions were observed frequently. @entity1162 stabilization was observed in four @entity1 but no objective @entity5 regressions were seen. Immune responses were measured in matched peripheral blood samples collected before and after treatment from 9 of 15 @entity1 treated at the 10(8) @entity5 cell dose. Four @entity1 exhibited MHC class I-restricted cytokine production in response to the @entity0 cell line. One @entity1 maintained an increased number of circulating @entity5 -specific, interferon gamma-secreting CD8(+) T cells for 24 months after the last vaccination. One @entity1 exhibited a @entity5 -specific @entity225 response to an @entity5 cell line. This immunization strategy proved to be safe and feasible, and induced @entity5 -specific immune responses in a minority of @entity1 ; however, no objective @entity5 regressions were observed.

Vaccination of XXXX with metastatic @entity0 , using a costimulatory gene ( @entity7938 )-modified, HLA-A2-matched, allogeneic, @entity0 cell line: clinical and immunological results.

@entity1 :: ('9606', 'Species') :: ['women', 'Patients', 'patients', 'patient'] @entity225 :: ('3567', 'Gene') :: ['interleukin 5'] @entity0 :: ('MESH:D001943', 'Disease') :: ['stage IV breast cancer', 'breast cancer', 'parental breast cancer'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'autologous tumor'] @entity7938 :: ('941', 'Gene') :: ['CD80'] @entity1162 :: ('MESH:D008133', 'Disease') :: ['Prolonged disease'] @entity2841 :: ('1437', 'Gene') :: ['GM-CSF', 'granulocyte-macrophage colony-stimulating factor'] @entity1722 :: ('2064', 'Gene') :: ['HER2']
@entity1 :: (9606,Species) :: ['women']
