UNASSIGNED: @entity2718 is one of the most dangerous pathogens in hospital settings. Most strains of @entity2718 carry one or more prophages and some of them, like   CD38-2 and   CD119, can influence the expression of toxin genes. However, little is known about the global host response in the presence of a given prophage. In order to fill this knowledge gap, we used RNA-seq to conduct a genome-wide transcriptomic analysis of the epidemic @entity68588 carrying the   CD38-2 prophage. A total of 39 bacterial genes were differentially expressed in the @entity68588 lysogen, 26 of them being downregulated. Several of the regulated genes encode transcriptional regulators and PTS subunits involved in @entity413 , @entity948 and @entity5121 / @entity5121 uptake and metabolism.   CD38-2 also upregulated the expression of a group of regulatory genes located in phi-027, a resident prophage common to most ribotype 027 isolates. The most differentially expressed gene was that encoding the conserved phase-variable cell wall protein CwpV, which was upregulated    20-fold in the lysogen. Quantitative PCR and immunofluorescence showed that the increased cwpV expression results from a greater proportion of cells actively transcribing the gene. Indeed,    95% of lysogenic cells express cwpV as opposed to only    5% of wild type cells. Furthermore, the higher proportion of cells expressing cwpV results from a higher frequency of recombination of the genetic switch controlling phase variation, which we confirmed to be dependent on the host-encoded recombinase RecV. In summary,   CD38-2 interferes with phase-variation of the surface protein CwpV and the expression of metabolic genes.

Global transcriptional response of XXXX carrying the phiCD38-2 prophage.

@entity5121 :: ('MESH:D013012', 'Chemical') :: ['sorbitol', 'glucitol'] @entity948 :: ('MESH:D005632', 'Chemical') :: ['fructose'] @entity68588 :: ('645463', 'Species') :: ['C. difficile strain R20291', 'R20291'] @entity2718 :: ('1496', 'Species') :: ['C. difficile', 'Clostridium difficile'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity2718 :: (1496,Species) :: ['Clostridium difficile']
