UNASSIGNED: @entity2531 ( @entity2531 ) has been studied as a model system to understand molecular events in physiology, biochemistry, and cytology during plant embryo development. In particular, it is exceedingly difficult to access the morphological and early regulatory events in zygotic embryos. To understand the molecular mechanisms regulating early @entity2531 in @entity27517 Maxim., we used high-throughput RNA-Seq technology to investigate its transcriptome. We obtained 58,327,688 reads, which were assembled into 75,803 unique unigenes. To better understand their functions, the unigenes were annotated using the Clusters of Orthologous Groups, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes databases. Digital gene expression libraries revealed differences in gene expression profiles at different developmental stages (embryogenic callus, yellow embryogenic callus, global embryo). We obtained a sequencing depth of >5.6 million tags per sample and identified many differentially expressed genes at various stages of @entity2531 . The initiation of @entity2531 affected gene expression in many KEGG pathways, but predominantly that in metabolic pathways, biosynthesis of secondary metabolites, and plant hormone signal transduction. This information on the changes in the multiple pathways related to @entity2531 induction in @entity27517 Maxim. embryogenic tissue will contribute to a more comprehensive understanding of the mechanisms involved in early @entity2531 . Additionally, the differentially expressed genes may act as molecular markers and could play very important roles in the early stage of @entity2531 . The results are a comprehensive molecular biology resource for investigating @entity2531 of @entity27517 Maxim.

Transcriptome profiling and digital gene expression by deep sequencing in early somatic embryogenesis of endangered medicinal XXXX Maxim.

@entity27517 :: ('82096', 'Species') :: ['Eleutherococcus senticosus', 'E. senticosus'] @entity2531 :: ('MESH:D013001', 'Disease') :: ['SE', 'Somatic embryogenesis']
@entity27517 :: (82096,Species) :: ['Eleutherococcus senticosus']
