Messenger @entity10731 encoding the alpha-subunit of @entity460 /calmodulin-dependent protein kinase II (camkII) is abundantly and constitutively expressed in dendrites of pyramidal and granule cell neurons of the adult hippocampus. Recent evidence suggests that camkII messenger @entity10731 is stored in a translationally dormant state within @entity10731 storage granules. Delivery of camkII messenger @entity10731 from sites of storage to sites of translation may therefore be a key step in activity-driven dendritic protein synthesis and synaptic plasticity. Here we explored possible camkII trafficking in the context of long-term potentiation in the dentate gyrus of awake, adult @entity35 . Long-term potentiation was induced by patterned high-frequency stimulation, synaptodendrosomes containing pinched-off dendritic spines were obtained from microdissected dentate gyrus, and messenger @entity10731 levels were determined by real-time polymerase chain reaction. High-frequency stimulation triggered a rapid 2.5-fold increase in camkII messenger @entity10731 levels in the synaptodendrosome fraction. This increase occurred in the absence of camkII upregulation in the homogenate fraction, indicating trafficking of pre-existing messenger @entity10731 to synaptodendrosomes. The elevation in camkII messenger @entity10731 was paralleled by an increase in protein expression specific to the synaptodendrosome fraction, and followed by depletion of camkII message. Activity-dependent regulation of camkII messenger @entity10731 and protein did not require @entity1024 receptor activation. In contrast, @entity1024 receptor activation was required for induction of the immediate early genes @entity11970 and activity-regulated cytoskeleton-associated protein in dentate gyrus homogenates. The results support a model in which locally stored camkII messenger @entity10731 is rapidly transported to dendritic spines and translated during long-term potentiation in behaving @entity35 .

Bursts of high-frequency stimulation trigger rapid delivery of pre-existing alpha-CaMKII mRNA to synapses: a mechanism in dendritic protein synthesis during long-term potentiation in adult awake XXXX .

@entity1024 :: ('CHEBI:31882', 'Chemical') :: ['N-methyl-d-aspartate'] @entity11970 :: ('24330(Tax:10116)', 'Gene') :: ['zif268'] @entity10731 :: ('CHEBI:33697', 'Chemical') :: ['ribonucleic acid'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity35 :: ('10116', 'Species') :: ['rats']
@entity35 :: (10116,Species) :: ['rats']
