UNASSIGNED: Vitis labrusca is a grapevine that has antioxidant, neuroprotective, hepatoprotective, and anticarcinogenic activity. However, the antithrombotic effect of Vitis labrusca leaves on platelets is yet to be ascertained. We investigated the inhibitory effect of V. labrusca leaf extract (VLE) on @entity1423 in vitro and ex vivo. The @entity7489 ( @entity3448 ) and @entity750 concentrations were measured by ELISA. The @entity3088 content was measured by ultraperformance liquid chromatography (UPLC). The antithrombotic activity of VLE was evaluated using various agonists in vitro. VLE strongly inhibited @entity857 ( @entity857 )-induced @entity1423 . In @entity35 , VLE treatment (100mg/kg) reduced @entity857 -stimulated @entity1423 , without affecting tail @entity548 and coagulation time. Moreover, VLE significantly suppressed @entity3448 and @entity750 secretion. UPLC analysis indicated that VLE contains @entity3092 , @entity58010 , and @entity15604 . Our results indicate that VLE possesses antiplatelet activity via the suppression of @entity3448 and @entity750 , without affecting @entity548 . Further, we identified the @entity3088 present in VLE. Thus, VLE may be a potential agent for the prevention of @entity65 .

Antithrombotic activity of Vitis labrusca extract on @entity35 XXXX .

@entity548 :: ('MESH:D006470', 'Disease') :: ['bleeding'] @entity1423 :: ('MESH:D001791', 'Disease') :: ['platelet aggregation'] @entity15604 :: ('MESH:D012431', 'Chemical') :: ['rutin'] @entity58010 :: ('MESH:C047368', 'Chemical') :: ['isorhamnetin'] @entity7489 :: ('MESH:D013929', 'Chemical') :: ['thromboxane B2'] @entity3448 :: ('CHEBI:28728', 'Chemical') :: ['TXB2'] @entity3088 :: ('MESH:D005419', 'Chemical') :: ['flavonoids'] @entity3092 :: ('MESH:D011794', 'Chemical') :: ['quercetin'] @entity750 :: ('MESH:D012701', 'Chemical') :: ['serotonin'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity857 :: ('MESH:D000244', 'Chemical') :: ['ADP', 'adenosine diphosphate'] @entity65 :: ('MESH:D002318', 'Disease') :: ['cardiovascular diseases']
@entity1423 :: (MESH:D001791,Disease) :: ['platelet aggregation']
