@entity31103 have been suggested to be mediators of many @entity842 -dependent processes, including apoptosis and vascular relaxation. @entity1432 nitrosation is a poorly understood process in vivo, and the mechanisms by which @entity842 can be converted into a nitrosating agent have not been established. There is a discrepancy between the suggested biological roles of @entity842 and its known chemical and physical properties. In this study, we have examined the formation of @entity31103 in lipopolysaccharide-treated RAW 264.7 cells. This treatment generated 17.4 +/- 1.0 pmol/mg of protein (means +/- SE, n =27) of intracellular @entity31103 that slowly decayed over several hours. @entity31103 formation depended on the formation of @entity842 and not on the presence of @entity3542 . Extracellular @entity1433 were nitrosated by cell-generated @entity842 . Oxygenated ferrous hemoglobin inhibited the formation of @entity31103 , indicating the nitrosation occurred more slowly than diffusion. We discuss several mechanisms for @entity31103 formation and conclude that the nitrosation propensity of @entity842 is a freely diffusible element that is not constrained within an individual cell and that both @entity842 per se and @entity842 -derived nitrosating agents are able to diffuse across cell membranes. To achieve intracellular localization of the nitrosation reaction, mechanisms must be invoked that do not involve the formation of @entity842 as an intermediate.

Formation and stability of XXXX in RAW 264.7 cells.

@entity1433 :: ('MESH:D013438', 'Chemical') :: ['thiols'] @entity3542 :: ('CHEBI:16301', 'Chemical') :: ['nitrite'] @entity1432 :: ('CHEBI:29256', 'Chemical') :: ['Thiol'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity31103 :: ('MESH:D026403', 'Chemical') :: ['S-Nitrosothiol', 'S-nitrosothiols', 'S-Nitrosothiols', 'S-nitrosothiol']
@entity31103 :: (MESH:D026403,Chemical) :: ['S-nitrosothiols']
