OBJECTIVE: The expression of survivin gene in @entity157 cell line SMMC-7721 was blocked by means of antisense oligonucleotide (ASODN) transfection. To investigate the signal transduction pathway of apoptosis induced by survivin ASODN. METHODS: survivin ASODN was transfected into SMMC-7721 cells mediated by @entity11505 liposomal reagent. The expression of survivin protein and mRNA was detected by western-blot and in situ hybridization method, respectively. Flow cytometer and TUNEL method were used to detect apoptosis. The changes in the expression and activity of p38MAPK and @entity522 were assessed by western-blot, immuno-precipitation, RT-PCR, and kinase activity assess to study the relationship between the changes of p38MAPK and @entity522 activity. RESULTS: The expression of survivin protein and mRNA decreased 84.6% and 69.7% respectively while apoptosis increased from 0.70% to 31.15% after transfected with survivin ASODN. P38MAPK activity increased significantly followed by increasing of @entity522 activity after survivin ASODN transfection. Both p38MAPK and @entity522 activity were inhibited after treated with inhibitor of p38MAPK, @entity4285 . CONCLUSION: Transfection of survivin ASODN could induce @entity157 cells apoptosis effectively through activated p38MAPK- @entity522 signal pathway sequentially.

[The signal transduction pathway related to XXXX apoptosis induced by survivin antisense oligonucleotide].

@entity157 :: ('MESH:D006528', 'Disease') :: ['hepatocellular carcinoma'] @entity11505 :: ('MESH:C070046', 'Chemical') :: ['DOTAP'] @entity522 :: ('836', 'Gene') :: ['caspase-3'] @entity4285 :: ('MESH:C090942', 'Chemical') :: ['SB202190']
@entity157 :: (MESH:D006528,Disease) :: ['hepatocellular carcinoma']
