AIMS: Different approaches have been described for reporting specimen adequacy for @entity470 ( @entity470 ) mutation analysis. We aimed: (1) to conduct cellularity assessment and to investigate its association with DNA yield, (2) to compare the H_E slides taken before and after the thick sections (curls) obtained for @entity470 testing and (3) to evaluate the number of ancillary studies performed. METHODS: Cell block (CB) slides of 110 @entity1149 cases submitted to @entity470 analysis from 2010 to 2012 were reviewed for total cellularity (ranges 1-100, 100-250, 250-500, 500-750, 750-1000 and >1000 cells), @entity5 cellularity (ranges 1-50, 50-100, 100-300 and >300 cells) and the percentage of @entity5 cells. Precurl and postcurl H_E slides were compared using the three criteria. The number of immunohistochemistry (IHC) markers and special stains and DNA yield were recorded. RESULTS: DNA yield was significantly associated with the total cellularity, number and percentage of @entity5 cells. For 46 cases with precurl and postcurl slides, only three (6.5%) were classified as being different and in two of them the postcurl slide had greater cellularity than the precurl. IHC was performed in 83 cases, with a minimum of 1 and a maximum of 11 markers (median of 3) per case. CONCLUSIONS: An association between the total cellularity and the @entity5 cellularity with the DNA yield was demonstrated using the ranges described. Evaluation of a postcurl slide is an unnecessary practice. The majority of the CB had sufficient material for ancillary studies (up to 11 markers) and mutation testing.

A proposal for cellularity assessment for @entity470 mutational analysis with a correlation with DNA yield and evaluation of the number of sections obtained from cell blocks for immunohistochemistry in XXXX .

@entity470 :: ('1956', 'Gene') :: ['EGFR', 'epidermal growth factor receptor'] @entity1149 :: ('MESH:D002289', 'Disease') :: ['non-small cell lung carcinoma'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumour']
@entity1149 :: (MESH:D002289,Disease) :: ['non-small cell lung carcinoma']
