AIMS/HYPOTHESIS: Autoantibodies against pancreatic islets and infections by enteroviruses are associated with type 1 @entity6 , but the specificity of immune responses within the type 1 @entity6 pancreas is poorly characterised. We investigated whether pancreatic lymph nodes could provide a source of antigen-specific B cells for analysis of immune responses within the (pre) @entity6 pancreas. METHODS: Human IgG antibodies were cloned from single B lymphocytes sorted from pancreatic lymph node cells of three organ donors positive for islet autoantibodies, and from the peripheral blood of a @entity1 with type 1 @entity6 . Antibodies to insulinoma-associated antigen 2 ( @entity9217 ), @entity1840 , @entity219 transporter 8 ( @entity5920 ) and Coxsackie @entity22421 proteins were assayed by immunoprecipitation and by immunofluorescence on pancreatic sections. RESULTS: Human IgG antibodies (863) were successfully cloned and produced from 4,092 single B cells from lymph nodes and peripheral blood. Reactivity to the protein @entity4149 phosphatase domain of the @entity9217 autoantigen was detected in two cloned antibodies: one derived from a pancreatic lymph node and one from peripheral blood. Epitopes for these two antibodies were similar to each other and to those for circulating antibodies in type 1 @entity6 . The remaining 861 antibodies were negative for reactivity to @entity9217 , @entity1840 or @entity5920 by both assays tested. Reactivity to a Coxsackie viral protein 2 was detected in one antibody derived from a peripheral blood B cell, but not from lymph nodes. CONCLUSIONS/INTERPRETATION: We show evidence for the infrequent presence of autoantigen-specific IgG(+) B lymphocytes in the pancreatic-draining lymph nodes of islet autoantibody-positive individuals.

Isolation of human monoclonal autoantibodies derived from pancreatic lymph node and peripheral blood B cells of islet autoantibody-positive XXXX .

@entity1 :: ('9606', 'Species') :: ['patient'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetic', 'diabetes'] @entity1840 :: ('2572', 'Gene') :: ['GAD65'] @entity22421 :: ('12286', 'Species') :: ['B virus'] @entity219 :: ('MESH:D015032', 'Chemical') :: ['zinc'] @entity5920 :: ('169026', 'Gene') :: ['ZnT8'] @entity9217 :: ('5798', 'Gene') :: ['IA-2'] @entity4149 :: ('D014443', 'Chemical') :: ['tyrosine']
@entity1 :: (9606,Species) :: ['patients']
