UNASSIGNED: The early persistence of @entity41 ( @entity41 ) is considered a poor prognostic factor indicative of chemoresistance in @entity981 . In French @entity1 , chemosensitivity is assessed at day 21 post-induction by cytomorphology. Here, it was investigated whether a more precise evaluation could be obtained at this time point with multiparameter flow cytometry (MFC). This study enrolled 123 @entity1 with de novo @entity981 . MRD0 was investigated at day 21 in MFC with a combination of antibodies based on the immunophenotype of diagnosis. It was also evaluated at day 35 by immunoglobulin/T-cell receptor quantitative real-time polymerase chain reaction ( @entity25796 ). Three risk groups could be delineated based on MRD0. @entity1 with MFC/MRD0 levels >10(-2) (n   =   25) were considered high risk, those with levels between 10(-2) and 10(-4) (n   =   46) intermediate risk, and those <10(-4) (n   =   50) low risk. Overall survival (p   =   0.048) and event-free survival (EFS, p   =   0.00017) were significantly different between these three groups. EFS of the 14 corticoresistant @entity1 strongly depended on their MRD0 level (p   =   0.004). Similarly, both EFS (p   =   0.0004) and overall survival (p   =   0.02) were significantly different in the 109 chemosensitive @entity1 , according to MRD0 levels. MRD0 and @entity25796 levels, compared with 112 @entity1 , were consistent (-/- or +/+) in 57.2% of the cases. Both MRD0+/ @entity25796 + and MRD0+/ @entity25796 - @entity1 had a significantly worse EFS (p   =   0.0001) than those with undetectable @entity41 at both MRD0 and @entity25796 . This study confirms the usefulness and superiority of an early point of @entity41 detection by MFC. In addition, MRD0 in MFC identifies a subgroup of @entity1 with poorer prognosis (MRD0+/ @entity25796 -). Copyright    2015 John Wiley _ Sons, Ltd.

Major impact of an early bone marrow checkpoint (day 21) for XXXX in flow cytometry in childhood @entity981 .

@entity1 :: ('9606', 'Species') :: ['children', 'Patients', 'patients'] @entity981 :: ('MESH:D054198', 'Disease') :: ['acute lymphoblastic leukemia'] @entity25796 :: ('55777', 'Gene') :: ['MRD1'] @entity41 :: ('MESH:D018365', 'Disease') :: ['MRD', 'minimal residual disease']
@entity41 :: (MESH:D018365,Disease) :: ['minimal residual disease']
