BACKGROUND: We measured the functional and metabolic status of hearts submitted to normothermic @entity63 before preservation through the use of an ex  vivo @entity1707 heart model to assess the feasibility of donation after @entity204 ( @entity6061 ) in heart transplantation. METHODS: Ten @entity1707 were separated into 2 groups: control (n  = 6, @entity1620 group) and @entity6061 (n  = 4, heart donation after @entity204 ). In the control group, hearts were excised 20 minutes after the brachiocephalic trunk cross-clamping and were immediately reperfused. In @entity6061 , hearts were excised 20 minutes after exsanguination and @entity1763 , stored in the Centre de R  sonance Magn  tique Biologique et M  dicale (CRMBM) solution for 2 hours, and then were reperfused. @entity1167 was induced with the use of 1 L of CRMBM solution (4  C) and the heart was reperfused for 60 minutes through the use of an ex  vivo perfusion system in Langendorff mode with normothermic autologous blood. During reperfusion, functional parameters were analyzed. Biochemical assays were performed in myocardial effluents and freeze-clamped hearts. RESULTS: No electromechanical activity was found in @entity6061 compared with control. @entity908 kinase (CK) was higher at 2 minutes of reperfusion in @entity6061 versus control (P  = .005). @entity855 was lower in @entity6061 versus control (P  = .0019). @entity1234 , an oxidative stress index, was present only in @entity6061 . The @entity842 (NO) pathway was impaired in @entity6061 versus control, with lower eNOS expression (P  < .0001) and total @entity1507 concentration content (P  = .04). CONCLUSIONS: We reported no cardiac functional and metabolic recovery in the @entity6061 group after normothermic @entity63 and reperfusion, which indicates that a single immersion of the cardiac graft during storage does not provide an optimal protection. New strategies in heart preservation are necessary for recruiting heart donation after @entity204 .

Heart donation after @entity204 : preliminary study on an isolated, perfused @entity1707 heart after 20 minutes of normothermic XXXX .

@entity1707 :: ('9823', 'Species') :: ['pigs', 'pig'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemia'] @entity908 :: ('MESH:D003401', 'Chemical') :: ['Creatine'] @entity1507 :: ('CHEBI:48107', 'Chemical') :: ['nitrate'] @entity1620 :: ('MESH:D001926', 'Disease') :: ['brain-dead'] @entity1234 :: ('MESH:D008315', 'Chemical') :: ['Malondialdehyde'] @entity6061 :: ('MESH:C018917', 'Chemical') :: ['DCD'] @entity1763 :: ('MESH:D001237', 'Disease') :: ['asphyxia'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity1167 :: ('MESH:D006323', 'Disease') :: ['Cardioplegic arrest'] @entity204 :: ('MESH:D003643', 'Disease') :: ['cardiac death'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['Adenosine triphosphate']
@entity63 :: (MESH:D007511,Disease) :: ['ischemia']
