This study developed a packaging method to integrate the extended-gate field-effect transistor (EGFET) into a microfluidic chip as a biological sensor. In addition, we present two immobilization approaches for the bio-recognition that are appropriate to this chip, allowing it to measure the concentrations of @entity3884 ions, @entity413 , @entity1133 , and specific proteins in a solution. Alginate- @entity460 microcubes were used to embed the enzymes and magnetic powder (enzyme carrier). When the sensing chip needs the enzyme for the catalytic reaction, the alginate microcubes containing the corresponding enzymes enter through the flow channel and are immobilized on the EGFET surface with an external magnet. High sensing performance of the chip is achieved, with 37.45  mV/mM for measuring @entity3884 ions at pH  6-8 with a linearity of 0.9939, 7.00  mV/mM for measuring @entity413 with a linearity of 0.9962, and 8.01  mV/mM for measuring @entity1133 with a linearity of 0.9809. In addition, based on the principle of the immunoassay, the magnetic beads with the specific antibody were used to capture the target protein in the sample. Then, negatively charged DNA fragments bound to a secondary antibody were used to amplify the signal for EGFET measurement. The magnetic beads with completed immune response bonding were then fixed on the surface of the sensor by an external magnetic field. Therefore, the measured object can directly contact the sensor surface, and quantitative detection of the protein concentration can be achieved. @entity1675 ( @entity1675 ) was detected as a target protein, with a minimum detection limit of approximately 12.5  ng/mL.

Extended-gate field-effect transistor packed in micro channel for @entity413 , XXXX and protein biomarker detection.

@entity3884 :: ('MESH:D006859', 'Chemical') :: ['hydrogen'] @entity1133 :: ('MESH:D014508', 'Chemical') :: ['urea'] @entity1675 :: ('335', 'Gene') :: ['Apolipoprotein A1', 'APOA1'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity1133 :: (MESH:D014508,Chemical) :: ['urea']
