OBJECTIVES: Neutrophil infiltration into the lung is the critical characteristic of @entity647 ( @entity647 ), which is a clinical state with acute inflammatory syndrome. Up to now, there is no effective medicine for @entity647 . @entity42095 has been shown to posses serval biological activities including anti- @entity32 , anti-oxidant and anti- @entity189 . METHODS: @entity647 was induced by intratracheal injection of @entity558 , and @entity42095 at various concentrations was injected intraperitoneally 30 min prior to @entity558 . Contents of @entity10873 ( @entity10873 ) and expression of chemokines and adhesion molecules were determined by commercially and ELISA assay kits, respectively. Akt phosphorylation and @entity22296 activation were measured by western blot and @entity22296 pull-down activation assay, respectively. KEY FINDING: Neutrophil infiltration was reduced by @entity42095 in a concentration-dependent manner in the @entity558 -induced @entity647 @entity19 model. @entity558 -induced proinflammatory cytokines and adhesion molecules were inhibited by @entity42095 in bronchoalveolar lavage fluid (BALF) with @entity558 -induced @entity647 . Furthermore, @entity42095 suppressed Akt phosphorylation and @entity22296 activation in lungs in @entity558 -induced @entity647 . The similar parallel trend was observed as @entity42095 reduced @entity558 -induced neutrophils infiltration, proinflammatory cytokines generation, adhesion molecules expression, Akt phosphorylation, and @entity22296 activation. SUMMARY: These results suggested that the effects of @entity42095 in @entity558 -induced @entity647 were induced by inhibition of Akt phosphorylation and @entity22296 activation.

@entity647 induced by lipopolysaccharide is inhibited by XXXX in @entity19 via reduction of Akt phosphorylation and @entity22296 activation.

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity558 :: ('MESH:C536528', 'Disease') :: ['LPS'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinoma'] @entity42095 :: ('MESH:C085514', 'Chemical') :: ['wogonin', 'Wogonin'] @entity22296 :: ('11848(Tax:10090)', 'Gene') :: ['RhoA'] @entity10873 :: ('17523(Tax:10090)', 'Gene') :: ['myeloperoxidase', 'MPO'] @entity647 :: ('MESH:D055371', 'Disease') :: ['acute lung injury', 'ALI', 'Acute lung injury']
@entity42095 :: (MESH:C085514,Chemical) :: ['wogonin']
