UNASSIGNED: We previously reported that @entity68876  ( @entity68877 ) induces apoptosis in @entity1 @entity320 UACC-62  cells. In the present study, the molecular mechanism of @entity68877  -induced apoptosis in @entity320 cells was investigated. Briefly, the apoptosis triggered by @entity68877 was confirmed in UACC  -62  cells for shorter treatment periods. Increased activities of @entity522 and @entity8187 but not @entity8186 were observed in the cell lysates. Western blotting showed that the pro  -apoptotic protein @entity589 was upregulated and the anti  -apoptotic protein @entity2423 was downregulated and @entity4674 (Cyto  c) was released into the cytosol. Flow cytometric analysis demonstrated that @entity68877 induced significant mitochondrial membrane potential disruption. Significant increases in the generation of reactive @entity26 species (ROS) and cytosolic @entity460 elevation were also observed. However, opening of the mitochondrial permeability transition pore which could be involved in Cyto  c leakage from mitochondria was found to be unaffected by @entity68877 . Taken together, all the results presented in this study including apoptotic induction, activation of the @entity9189 cascade, upregulation of @entity589 , downregulation of @entity2423 , Cyto  c release from the mitochondria, mitochondrial membrane potential depletion, ROS production and cytosolic @entity460 elevation demonstrated that @entity68877 triggered apoptosis in the UACC  -62  cells via the mitochondrial  -dependent pathway. @entity320 is well  -known as an @entity146 and highly metastatic disease. In this study, we also investigated the effects of @entity68877 on the migration of UACC  -62 cells using the xCELLigence system. The results revealed that in  vitro @entity68877 is capable of inhibiting the migration of @entity320 cells.

XXXX induces mitochondrial-mediated apoptosis and suppresses migration in @entity320 cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity9189 :: ('836;842', 'Gene') :: ['caspase  -3 and   -9'] @entity522 :: ('836', 'Gene') :: ['caspase  -3'] @entity589 :: ('581', 'Gene') :: ['Bax'] @entity146 :: ('MESH:D001523', 'Disease') :: ['aggressive'] @entity2423 :: ('4170', 'Gene') :: ['Mcl  -1'] @entity4674 :: ('54205', 'Gene') :: ['cytochrome  c'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity68877 :: ('CHEBI:55387', 'Chemical') :: ['NACC'] @entity8187 :: ('842', 'Gene') :: ['caspase  -9'] @entity8186 :: ('841', 'Gene') :: ['caspase  -8'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity68876 :: ('MESH:C454682', 'Chemical') :: ['N-acetyl-S-(p-chlorophenylcarbamoyl)cysteine'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma', 'Melanoma']
@entity68876 :: (MESH:C454682,Chemical) :: ['N  -acetyl-S-(p-chlorophenylcarbamoyl)cysteine']
