@entity5165 has been shown to affect the male reproductive organs. However, the specific mode of impairment of spermatogenesis during @entity5165 exposure remains unknown. In this study, we characterized the induction of germ cell apoptosis and reproductive @entity137 in @entity35 male @entity35 that had been exposed to @entity3319 ( @entity1139 ). Subcutaneous injection of @entity1139 at a dose of 10 mg/kg per day for 8 days resulted in a 28% testicular weight loss at 14 days after the first injection. In addition, the ventral and dorso-lateral prostatic lobes showed a 65 and 52% decrease, respectively, at 14 days, although no effects were observed in the epididymis. Sperm production also was suppressed by the administration of @entity1139 . After exposure to @entity1139 , fragmentation of testicular DNA was found to be increased at 3 days after the first injection, with a 20-fold increase over control levels at 14 days. In situ detection of apoptosis by @entity12809 ( @entity12809 )-mediated @entity1977 nick end-labeling (TUNEL) staining revealed that spermatocytes and spermatids at stages VII-VIII and IX-XI, respectively, steps which are considered to be highly sensitive to @entity251 , were the major cell types affected. Consequently, a marked cell loss in elongated spermatids at stages XII-XIV and I was observed at 14 days. In addition, plasma @entity251 levels were reduced at 6 days after exposure to @entity1139 , and remained at approximately 20% of control levels during the 14-day observation period. Our results suggest that @entity5165 impairs spermatogenesis by germ cell deletion via cell- and stage- specific apoptosis.

Impairment of spermatogenesis in @entity35 by XXXX : involvement of stage- and cell- specific germ cell apoptosis.

@entity251 :: ('MESH:D013739', 'Chemical') :: ['testosterone'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity12809 :: ('294051(Tax:10116)', 'Gene') :: ['terminal deoxynucleotidyl transferase', 'TdT'] @entity1977 :: ('CHEBI:17625', 'Chemical') :: ['dUTP'] @entity1139 :: ('CHEBI:27504', 'Chemical') :: ['MMC'] @entity3319 :: ('MESH:C004925', 'Chemical') :: ['methylmercuric chloride'] @entity35 :: ('10116', 'Species') :: ['Wistar', 'rats'] @entity5165 :: ('CHEBI:30785', 'Chemical') :: ['methylmercury', 'Methylmercury']
@entity5165 :: (CHEBI:30785,Chemical) :: ['methylmercury']
