AIM: To evaluate robustness, prebiotic utilisation of @entity6983 F8 and Lact. plantarum F44 in mono- and co-cultures with @entity14914 46 and @entity14320 sub sp. lactis 8:8 and antimicrobial activity of co-culture against @entity2718 . METHODS AND RESULTS: The two @entity6983 strains showed a high acid and bile tolerance. Lact. plantarum F44 showed maximum growth in @entity7748 basal broth with @entity413 and @entity11884 compared to growth in galacto-oligosaccharides (GOS) and isomalto-oligosaccharides (IMOS). In co-culture system, the amylolytic @entity14914 46 stimulated the growth of a non-amylolytic Lact. paracasei F8, probably by producing intermediate metabolites of starch metabolism. A higher growth of four strains Lact. paracasei F8, Lact. plantarum F44, @entity14914 46 and @entity14320 ssp lactis 8:8 with different prebiotic combinations was found in a MRSC basal broth with @entity12360 ( @entity12360 ) + IMOS + GOS and IMOS + GOS respectively. The two @entity6983 strains exhibited a high antimicrobial activity against four clinical @entity2718 strains and a hypervirulent NAP1/027strain and suppressed the toxin titres possibly through the production of organic acids and heat stable antimicrobial proteins when grown on @entity413 and through the production of acids when grown on prebiotics. Culture supernatants from synbiotic combinations inhibited the growth of the @entity2718 NAP1/027 strain and its toxin titres. CONCLUSION: Lact. paracasei F8, Lact. plantarum F44 exhibited a potential probiotic properties. Further, the two @entity6983 and two bifidobacteria strains were compatible with each other and exhibited high growth in co-cultures in presence of prebiotics and @entity12360 and antimicrobial activity against clinical @entity2718 strains and a hypervirulent NAPI/027 strain. This article is protected by copyright. All rights reserved.

Prebiotic preferences of human lactobacilli strains in co-culture with bifidobacteria and antimicrobial activity against XXXX .

@entity14320 :: ('28025', 'Species') :: ['B. animalis'] @entity11884 :: ('MESH:D007792', 'Chemical') :: ['lactulose'] @entity7748 :: ('MESH:D008556', 'Disease') :: ['MRS'] @entity14914 :: ('1685', 'Species') :: ['Bifidobacterium breve', 'B. breve'] @entity6983 :: ('1597', 'Species') :: ['Lactobacillus paracasei', 'Lactobacillus'] @entity12360 :: ('MESH:C565532', 'Disease') :: ['soluble starch', 'SS'] @entity2718 :: ('1496', 'Species') :: ['C. difficile', 'Clostridium difficile'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity2718 :: (1496,Species) :: ['Clostridium difficile']
