The present work which was investigated in July 1979, was to observe the wormicidal effects of the external mucous substances of 9 species of fresh water fishes ( @entity7136 , @entity17370 , @entity16932 , @entity43683 , @entity30947 ; golden crusian @entity7136 , @entity38823 , Zacco @entity429 , @entity12109 and @entity30947 ; crusian @entity7136 ) on cercaria, liberated metacercaria and adult of @entity2440 . The mucous substances extracted by ether from the above 9 species of fishes were separated into many spots on the @entity3440 gel thin layer chromatography, and divided into many fractions in the @entity3440 gel column chromatography which used @entity8144 (30%) and @entity3636 (70%) as a solvent. 1. On the @entity3440 gel thin layer chromatography of ether extracts from 9 species of fresh water fishes, each of the Rf. values which had wormicidal effect on the cercaria of @entity2441 were different from others; Rf. value of @entity30947 (crusian @entity7136 ) was 0.937, 0.709 in @entity43683 , 0.612 in A. japonica, 0.576 in @entity17370 , 0.451 in @entity7136 , 0.701 in Z. @entity429 , 0.385 in @entity30947 (golden crusian @entity7136 ) and 0.15 in @entity12109 . Time for wormicide was different from each other, too. It took 14 min. in a case of @entity30947 (crusian @entity7136 ), 25 min. in Z. @entity429 , 26 min. in @entity30947 (golden crusian @entity7136 ), 28 min. in @entity7136 , 30 min. in @entity17370 , 35 min. in @entity43683 , 40 min. @entity12109 and 180 min. in A. japonica. But any of the spots of @entity38823 did not show wormicidal effect on the cercaria within 10 hours. 2. In the @entity3440 gel column chromatography of ether extracts from 9 species of fresh water fishes, the fractions which had wormicidal effect on the metacercaria of C. sienesis were different from each other; in a case of @entity30947 (crusian @entity7136 ), the first fraction only had wormicidal effect, the 2nd in @entity17370 , the 3rd in @entity43683 , the 4th in @entity30947 (golden crusian @entity7136 ), the 2nd in @entity7136 and the 4th in @entity12109 . Time for wormicide was 40 min. in @entity30947 (crusian @entity7136 ), 52 min. in @entity17370 , 74 min. in @entity30947 (golden crusian @entity7136 ), 92 min. in @entity43683 , 95 min. in @entity7136 and 140 min. in @entity12109 , but any of the fractions of A. japonicas, @entity38823 and Z. @entity429 did not show wormicidal effect within 10 hours. 3. The fractions which had wormicidal effect on the metacercaria of @entity2441 had also the same effect on the adult, but it took longer time to kill them. It took 3 hours in a case of @entity30947 (crusian @entity7136 ), 4 hrs. in @entity30947 (golden crusian @entity7136 ), 5 hrs. in @entity17370 , 6 hrs. and 6 min. in @entity43683 , 6 hrs. and 10 min. in @entity7136 and half and 6 hrs. in @entity12109 . But any of the fractions of A. japonica, @entity38823 and Z. @entity429 did not show wormicidal effect within 24 hrs.

[The Wormicidal Substances Of Fresh Water Fishes On XXXX : II. Preliminary Research On The Wormicidal Substances From Mucous Substances Of Various Fresh Water Fishes]

@entity17370 :: ('30991', 'Species') :: ['Parasilurus asotus', 'P. asotus'] @entity43683 :: ('215402', 'Species') :: ['Ophicephalus argus', 'O. argus'] @entity2440 :: ('79923', 'Species') :: ['Clonorchis sinensis'] @entity3636 :: ('MESH:D002725', 'Chemical') :: ['chloroform'] @entity16932 :: ('7937', 'Species') :: ['Anguilla japonica'] @entity38823 :: ('75329', 'Species') :: ['Misgurnus anguillicaudatus', 'M. anguillicaudatus'] @entity8144 :: ('MESH:C004544', 'Chemical') :: ['petroleum ether'] @entity2441 :: ('143401', 'Species') :: ['C. sinensis'] @entity3440 :: ('MESH:D012822', 'Chemical') :: ['silica'] @entity12109 :: ('51549', 'Species') :: ['Pseudorasbora parva', 'P. parva'] @entity429 :: ('9258', 'Species') :: ['platypus'] @entity7136 :: ('7962', 'Species') :: ['C. carpio', 'carp', 'Cyprinus carpio'] @entity30947 :: ('217509', 'Species') :: ['C. carassius', 'Carassius carassius']
@entity2440 :: (79923,Species) :: ['Clonorchis Sinensis']
