OBJECTIVE: To investigate the effects of liposomal formulation on @entity3413 nano-liposomes (SMV-liposome) promoting the osteogenic differentiation of @entity19 bone marrow stromal cells (BMSC) analyzed by the expressions of @entity8385 ( @entity8385 ) and @entity4972 ( @entity4972 ). METHODS: Primary BMDC were cultured in vitro using adherence and culture of whole bone marrow method. SMV dosage was set as control group and had two different dosages in this group on the basis of the concentration of SMV. 1  mol/L and 2  mol/L SMV concentration were represented by SMV low dosage group (S1) and SMV high dosage group (S2), respectively. Similarly, SMV-liposome dosage was set as experimental group including two different dosages, 1  mol/L SMV capsuled concentration as SMV-liposome low dosage group ( @entity18238 ) and 2  mol/L SMV capsuled concentration as SMV-liposome high dosage group ( @entity22723 ). Besides, groups with no drug intervention in the experiments were set as blank. BMSC were treated with different concentrations of SMV and SMV-liposome for 48 h, the activity of @entity4972 was measured using p-nitropheny-phosate method, and @entity4972 expression in the BMSC cells was stained by BCIP/NBT @entity4972 color development kit (BCIP/NBT Kit). Furthermore, @entity8385 expression in the BMSC was determined by Western Blot. RESULTS: @entity98 assay showed, after incubated with different concentrations of SMV and SMV-liposome, the cell viabilities of BMSC were all above 85% and had no significant difference in the groups. Compared with the same dosage of SMV in these groups, control group and experimental group had significantly elevated the specific activity of @entity4972 , the staining of BCIP/NBTKit as well as the protein expression of @entity8385 . Besides, the data showed dose-dependent elevation in the control group and experimental group, namely the high dose group had better results than the low dose group. CONCLUSION: Nano-liposomal formulation significantly enhanced SMV effects on the osteogenetic differentiation of BMSC.

[Effects of XXXX nano-liposomes on osteogenic differentiation of bone marrow stromal cells].

@entity18238 :: ('17392(Tax:10090)', 'Gene') :: ['SL1'] @entity3413 :: ('MESH:D019821', 'Chemical') :: ['simvastatin'] @entity4972 :: ('MESH:C562645', 'Disease') :: ['ALP', 'alkaline phosphatase'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity22723 :: ('17384(Tax:10090)', 'Gene') :: ['SL2'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity8385 :: ('12156(Tax:10090)', 'Gene') :: ['bone morphogenetic protein 2', 'BMP-2']
@entity3413 :: (MESH:D019821,Chemical) :: ['simvastatin']
