@entity3900 ( @entity3900 ) is a key enzyme involved in the release of @entity1426 and subsequent production of @entity2918 (PGs), which play important roles in regulating ovarian function, including mitogenic signalling by transforming growth factor (TGF) alpha. @entity3901 ( @entity3901 ), a newly identified member of the @entity3900 family, is distinct from the well-characterized, secreted 14-kDa group I and II @entity3900 in that @entity3901 selectively cleaves @entity1426 -containing phospholipids at the sn-2 position. Our previous studies have demonstrated that @entity7790 stimulates and @entity2533 suppresses PG production in hen granulosa cells during ovarian follicular development. The present work extends these findings. A @entity3901 is present as a 100-kDa protein doublet and has a transcript size of 3.2 kilobases (kb) in hen granulosa cells from the largest (F1) and the fifth and sixth ( @entity742 ) preovulatory follicles. Treatment of these cells with @entity7790 caused a shift from an electrophoretically fast-migrating to slow-migrating protein, a phenomenon sensitive to inhibitors of @entity563 / @entity1889 kinase as well as mitogen-activated protein (MAP) kinase pathways and indicative of phosphorylation and activation of @entity3901 . In contrast, @entity2533 suppressed @entity3901 expression, as evidenced by a marked decrease of @entity3901 mRNA abundance and protein content, but failed to prevent the mobility shift of @entity3901 induced by @entity7790 . Consistent with the influence of this growth factor on PG production observed previously and in contrast to the action of @entity7790 , the inhibition of @entity3901 by @entity2533 is more pronounced in granulosa cells at the early stage of follicular development. Our results demonstrate, for the first time, the regulation of granulosa cell @entity3901 at the transcriptional and posttranscriptional levels at different stages of follicular development. We propose that the activation by @entity7790 and suppression by @entity2533 of @entity3901 may be important regulatory mechanisms in the control of granulosa cell PG production and thereby the mitogenic response of the cells to the growth factors during ovarian follicular development.

Regulation of cytosolic XXXX in hen granulosa cells by transforming growth factors at different stages of follicular development.

@entity7790 :: ('7039', 'Gene') :: ['TGF alpha'] @entity2918 :: ('MESH:D011453', 'Chemical') :: ['prostaglandins'] @entity1426 :: ('MESH:D016718', 'Chemical') :: ['arachidonic acid'] @entity742 :: ('9940', 'Gene') :: ['F5-6'] @entity3900 :: ('151056', 'Gene') :: ['PLA2', 'Phospholipase A2'] @entity2533 :: ('7040', 'Gene') :: ['TGF beta'] @entity3901 :: ('5321', 'Gene') :: ['cPLA2', 'Cytosolic PLA2'] @entity563 :: ('CHEBI:17822', 'Chemical') :: ['serine'] @entity1889 :: ('CHEBI:26986', 'Chemical') :: ['threonine']
@entity3900 :: (151056,Gene) :: ['phospholipase A2']
