UNASSIGNED: Despite increasing evidence that @entity528 is an early event in the development of @entity626 , relatively little is known regarding the sequence or functional implications of cytokine-induced meniscal degradation or how degradation varies with age. This study examined dose-dependent patterns of interleukin-1 (IL-1)-induced matrix degradation in explants from the radially middle regions of juvenile and adult @entity291 menisci. Tissue explants were cultured for 10 days in the presence of 0, 1.25, 5 or 20ng/ml recombinant @entity1 @entity1192 . Juvenile explants exhibited immediate and extensive sulfated glycosaminoglycan (sGAG) loss and collagen release beginning after 4-6 days, with relatively little IL-1 dose-dependence. Adult explants exhibited a more graded response to IL-1, with dose-dependent sGAG release and a lower fraction of sGAG released (but greater absolute release) than juvenile explants. In contrast to juvenile explants, adult explants exhibited minimal collagen release over the 10-day culture. Compressive and shear moduli reflected the changes in explant composition, with substantial decreases for both ages but a greater relative decrease in juvenile tissue. Dynamic moduli exhibited stronger dependence on explant sGAG content for juvenile tissue, likely reflecting concomitant changes to both proteoglycan and collagen tissue components. The patterns of tissue degradation suggest that, like in articular cartilage, meniscal proteoglycans may partially protect collagen from cell-mediated degeneration. A more detailed view of functional changes in meniscal tissue mechanics with degeneration will help to establish the relevance of in vitro culture models and will advance understanding of how @entity528 contributes to overall joint changes in early stage @entity571 . This article is protected by copyright. All rights reserved.

XXXX meniscal tissue exhibits age- and interleukin-1 dose-dependent degradation patterns and composition-function relationships.

@entity1 :: ('9606', 'Species') :: ['human'] @entity626 :: ('MESH:D020370', 'Disease') :: ['knee osteoarthritis'] @entity571 :: ('MESH:D010003', 'Disease') :: ['osteoarthritis'] @entity1192 :: ('3552', 'Gene') :: ['IL-1a'] @entity291 :: ('9913', 'Species') :: ['bovine'] @entity528 :: ('MESH:D009410', 'Disease') :: ['meniscal degeneration']
@entity291 :: (9913,Species) :: ['Bovine']
