UNASSIGNED: Trichoderma harzianum is a fungus well known for its potential as a biocontrol agent against many fungal phytopathogens. The aim of this study was to characterize the proteins secreted by T. harzianum ALL42 when its spores were inoculated and incubated for 48 hours in culture media supplemented with @entity413 ( @entity367 ) or with cell walls from @entity10437 (FSCW), a phytopathogen that causes severe losses in common bean and soy crops in Brazil, as well as other crop diseases around the world. T. harzianum was able to grow in Trichoderma Liquid Enzyme Production medium (TLE) and Minimal medium (MM) supplemented with FSCW and in TLE+ @entity367 , but was unable to grow in MM+ @entity367 medium. Protein quantification showed that TLE+FSCW and MM+FSCW had 45- and 30- fold, respectively, higher protein concentration on supernatant when compared to TLE+ @entity367 , and this difference was observable on 2-D gel electrophoresis (2-DE). A total of 94 out of 105 proteins excised from 2-DE maps were identified. The only protein observed in all three conditions was epl1. In the media supplemented with FSCW, different hydrolases such as chitinases, b-1,3-glucanases, glucoamylases, a-1,3-glucanases, and proteases were identified, along with other proteins with no known functions in mycoparasitism, such as npp1 and cys. T. harzianum showed a complex and diverse arsenal of proteins that are secreted in response to the presence of FSCW, with novel proteins not previously described in mycoparasitic-related studies. This article is protected by copyright. All rights reserved.

Secretome analysis of the mycoparasitic fungus Trichoderma harzianum ALL 42 cultivated in different media supplemented with @entity10437 cell wall or XXXX .

@entity10437 :: ('169388', 'Species') :: ['Fusarium solani'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity367 :: ('CHEBI:18237', 'Chemical') :: ['GLU']
@entity413 :: (MESH:D005947,Chemical) :: ['glucose']
