UNASSIGNED: @entity15250 strains isolated from hot spring water were tested for @entity734 ( @entity734 ) production using a target panel of closely related microorganisms and other Gram-positive and Gram-negative bacteria. Molecular identification was carried out through specific PCR and 16S RNA sequence analysis. Isolates were identified as Brevundimonas diminuta and @entity15250 , the latter exhibited antimicrobial activity. @entity15250 strains produce an inhibitory substance against other @entity15250 strains and other species including food-borne pathogens. The @entity734 was sensitive to the proteolytic action of proteinase K, pronase E and trypsin but resistant to a-amylase, RNase and lipase C, reflecting its proteinaceous nature. The @entity734 was stable at 100    C and also after thermal treatment at 121    C for 15  min. Additionally, it was stable within a wide range of pH (2-10). The substance from @entity15250 T01 strain was bactericidal to @entity526 . SDS-PAGE analysis of the partial purified supernatant of strain T01 revealed a @entity734 with an approximate molecular mass of 8  kDa. Therefore, the results of this study show that @entity15250 T01 produces a @entity734 with a higher activity spectrum, which may find application in @entity1 medicine and in minimally processed food preservation.

Detection and characterization of a bacteriocin, putadicin T01, produced by XXXX isolated from hot spring water.

@entity526 :: ('562', 'Species') :: ['Escherichia coli'] @entity1 :: ('9606', 'Species') :: ['human'] @entity734 :: ('MESH:D019966', 'Disease') :: ['BLS', 'bacteriocin-like substance'] @entity15250 :: ('303', 'Species') :: ['P. putida strain', 'Pseudomonas', 'Pseudomonas putida', 'P. putida']
@entity15250 :: (303,Species) :: ['Pseudomonas putida']
