BACKGROUND: The aim of this study was to compare the inward permeability of the blood- @entity5995 barrier in healthy subjects from six European cities. METHODS: Seventy-two healthy subjects (age 20-70 years) were selected. At 30 min and 60 min after @entity941 injection, @entity941 mass in vitreous was calculated from the concentrations measured along the optical @entity735 . Non-protein-bound @entity941 (NPBF) concentrations were measured in plasma prepared from blood samples taken 7, 15 and 55 min after injection. Blood- @entity5995 barrier permeability (PBRB) was calculated from the vitreous @entity941 mass and the time integral of NPBF and was corrected for the autofluorescence of ocular tissue and for lenticular light transmittance. RESULTS: Mean PBRB values +/- SD (nm.s-1) were 2.07 +/- 0.54 (Coimbra), 2.01 +/- 0.43 (Frankfurt), 2.24 +/- 0.50 (Ghent), 2.37 +/- 0.56 (Herlev), 1.89 +/- 0.44 (Leiden) and 1.74 +/- 0.38 (Porto). Differences between centers were not significant (P > 0.35). Measurements were reproducible and independent of the time after @entity941 injection (P > 0.50). A PBRB higher than 3.16 nm.s-1 or a value which had increased by 32% was considered abnormal (P < 0.05). CONCLUSION: PBRB values were similar in all centers. The results demonstrate that this is a highly sensitive and reliable method for measuring the permeability of the blood- @entity5995 barrier.

Permeability of the blood- XXXX barrier in healthy humans. European Concerted Action on Ocular Fluorometry.

@entity735 :: ('MESH:D005124', 'Disease') :: ['axis of the eye'] @entity941 :: ('MESH:D019793', 'Chemical') :: ['fluorescein'] @entity5995 :: ('MESH:D012172', 'Chemical') :: ['retinal']
@entity5995 :: (MESH:D012172,Chemical) :: ['retinal']
