BACKGROUND: @entity171 (BP) increase bone mass in @entity1 with @entity309 and are effective in the prevention and treatment of @entity134 -induced @entity170 . However, little is known about their direct effects on chondrocytes. OBJECTIVES: To study the influence of BP on articular chondrocytes in vitro and to investigate whether BP can prevent @entity134 -induced apoptosis of articular chondrocytes. METHODS: @entity291 articular chondrocytes were cultured and incubated with different concentrations of @entity19160 , @entity5279 , @entity562 , or @entity619 . In the second part of the study, BP were added to the chondrocyte cultures one hour before co-incubation with @entity619 . Viability and proliferation were evaluated using @entity2667 staining and @entity4463 labelled @entity1014 incorporation. Apoptosis was measured with annexin V staining or the TUNEL method. RESULTS: Only high concentrations (>10(-6) mol/l) of @entity19160 , @entity5279 , and @entity562 induced a decrease in the viability and proliferation of chondrocytes. None of the BP at concentrations ranging from 10(-12) to 10(-3) mol/l induced apoptosis. @entity851 and apoptosis induced by @entity619 (10(-7) mol/l) was prevented by addition of @entity5279 (10(-6) mol/l) or @entity562 (10(-8) or 10(-6) mol/l). CONCLUSION: @entity171 in therapeutic concentrations are safe for articular chondrocytes in vitro. Moreover, @entity5279 and @entity562 prevent @entity619 -induced @entity851 and apoptosis of chondrocytes. These findings add evidence for a chondroprotective effect of @entity1568 -containing BP, especially in @entity1 treated with @entity288 .

Effect of @entity171 on viability, proliferation, and XXXX -induced apoptosis of articular chondrocytes.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity851 :: ('MESH:D006130', 'Disease') :: ['Growth retardation', 'growth retardation'] @entity1568 :: ('MESH:D009584', 'Chemical') :: ['nitrogen'] @entity171 :: ('MESH:D004164', 'Chemical') :: ['Bisphosphonates'] @entity5279 :: ('MESH:C019248', 'Chemical') :: ['pamidronate'] @entity2667 :: ('MESH:D011419', 'Chemical') :: ['propidium iodide'] @entity170 :: ('MESH:D010024', 'Disease') :: ['osteoporosis'] @entity134 :: ('CHEBI:35341', 'Chemical') :: ['steroid'] @entity562 :: ('MESH:C073008', 'Chemical') :: ['risedronate'] @entity619 :: ('MESH:D003907', 'Chemical') :: ['dexamethasone'] @entity4463 :: ('CHEBI:29304', 'Chemical') :: ['tritium'] @entity1014 :: ('MESH:D013936', 'Chemical') :: ['thymidine'] @entity19160 :: ('MESH:D004002', 'Chemical') :: ['clodronate'] @entity291 :: ('9913', 'Species') :: ['Bovine'] @entity309 :: ('MESH:D001172', 'Disease') :: ['rheumatoid arthritis'] @entity288 :: ('MESH:D000305', 'Chemical') :: ['corticosteroids']
@entity619 :: (MESH:D003907,Chemical) :: ['dexamethasone']
