UNASSIGNED: Background: Ultrasound (US) molecular imaging has shown promise in assessing @entity32 in preclinical, @entity19 models of @entity29 . These models, however, initiated acute @entity32 on previously normal colons, in contrast to @entity1 where acute exacerbations are often in chronically inflamed regions. In this study, we explored the potential of dual P- and @entity24780 targeted US imaging for assessing acute @entity32 on a @entity19 quiescent chronic inflammatory background. Methods: @entity1960 was induced using three cycles of 4% DSS in male FVB @entity19 . Acute @entity32 was initiated 2 weeks after the final DSS cycle through rectal administration of 1% @entity10776 . @entity19 at different stages of @entity32 were imaged using a small animal ultrasound system following i.v. injection of microbubbles targeted to P- and @entity24780 . In vivo imaging results were correlated with ex vivo immunofluorescence and histology. Results: Induction of acute @entity32 resulted in an increase in the targeted US signal from 5.5    5.1 arbitrary units (a.u.) at day 0 to 61.0    45.2 a.u. (P < 0.0001) at day 1, 36.3    33.1 a.u. at day 3, returning to levels similar to control at day 5. Immunofluorescence showed significant increase in the percentage of P- and @entity24780 positive vessels at day 1 (P-selectin: 21.0    7.1% of vessels; P < 0.05; @entity24780 : 16.4   3.7%; P < 0.05) compared to day 0 (P-selectin: 10.3    5.7%; @entity24780 : 7.3    7.0%). Conclusions: Acute @entity32 can be accurately measured in a clinically relevant @entity19 model of chronic IBD using ultrasound molecular imaging with a dual P- and @entity24780 -targeted contrast agent.

Assessment of Inflammation in an Acute on Chronic Model of XXXX with Ultrasound Molecular Imaging.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity24780 :: ('20339(Tax:10090)', 'Gene') :: ['E-selectin', 'E- selectin'] @entity19 :: ('10090', 'Species') :: ['mice', 'Mice', 'murine'] @entity29 :: ('MESH:D015212', 'Disease') :: ['inflammatory bowel disease'] @entity1960 :: ('MESH:D003092', 'Disease') :: ['Chronic colitis'] @entity10776 :: ('CHEBI:53063', 'Chemical') :: ['TNBS']
@entity29 :: (MESH:D015212,Disease) :: ['Inflammatory Bowel Disease']
