OBJECTIVE: To explore the pneumoperitoneum-mediated renoprotective effects of preconditioning, mobilizing and homing of endothelial progenitor cells (EPCs) in @entity35 . METHODS: A total of 40 @entity35 were randomized by a numerical table into 5 groups of gasless (C), @entity130 ( @entity130 ), long-term pneumoperitoneum preconditioning (P-L), mid-term pneumoperitoneum preconditioning (P-M) and short-term pneumoperitoneum preconditioning (P-S). C group had a pneumoperitoneum pressure of 0 mmHg; @entity130 group 15 mmHg, time 60 min; P-L, P-M, P-S groups were deflated and deflated preconditioning before pneumoperitoneum, then the same as @entity130 group, P-L group: inflation time was 25 min, gas discharge time 10 min; P-M group: 15 min, 10 min; P-S group: 5 min, 10 min. At 24 h post-operation, the animals were sacrificed by destroying cervical spine. And the specimens of venous blood and kidneys were harvested. Also the extent of @entity8 , the homing of EPCs, the proliferation and angiogenesis of renal endothelial cell and the expression of angiogenic growth factor were analyzed. RESULTS: Compared with @entity130 group, P-L, P-M and P-S groups exhibited significant improvements in renal function, morphology and histological score (1.88    0.35, 1.63    0.52, 1.75    0.46 vs 2.38    0.52, all P < 0.05). The histological scores of P-M and P-S groups improved significantly versus P-L group (both P < 0.05). P-M and P-S groups showed no significant difference in histological score (P > 0.05). The number of EPCs in kidneys increased in P-L, P-M and P-S groups versus @entity130 group (2.18%    0.14%, 2.87%    0.29%, 2.90%    0.24% vs 1.73%    0.19%, all P < 0.05). The EPCs numbers of P-M and P-S groups were more than that of P-L group (both P < 0.05). And no significant difference existed between P-M and P-S groups (P > 0.05). Compared with @entity130 group, EPCs of P-L, P-M and P-S groups markedly increased in kidneys. No significant difference existed between P-M and P-S groups, but P-L group was the lowest. Also there was an up-regulated expression of stromal cell derived factor 1-a in pretreated kidneys versus @entity130 group (all P < 0.05). And P-M and P-S groups increased markedly. CONCLUSIONS: Pneumoperitoneum-mediated preconditioning protects against @entity8 by promoting EPC homing and enhancing endothelial cell and vascular proliferations. And short and medium-term preconditioning protocols are more effective for protecting kidneys.

[Effects of pneumoperitoneum preconditioning on endothelial progenitor cells and renal protective mechanism in XXXX ].

@entity35 :: ('10116', 'Species') :: ['rats'] @entity8 :: ('MESH:D007674', 'Disease') :: ['kidney injury', 'renal injury'] @entity130 :: ('MESH:D014947', 'Disease') :: ['Pp', 'pneumoperitoneum injury']
@entity35 :: (10116,Species) :: ['rats']
