A signaling cascade that includes @entity7131 ( @entity7131 ), Ras, and @entity44985 regulates @entity40330 ( @entity41579 ) gene expression in epidermal keratinocytes (Efimova, T., LaCelle, P., Welter, J. F., and Eckert, R. L. (1998) J. Biol. Chem. 273, 24387-24395 and Efimova, T., and Eckert, R. L. (2000) J. Biol. Chem. 275, 1601-1607). Because signal transfer downstream of @entity44985 may involve several MAPK kinases (MEKs), it is important to evaluate the regulatory role of each MEK isoform. In the present study we evaluate the role of @entity32027 in transmitting this signal. Constitutively active @entity32027 (caMEK6) increases @entity41579 promoter activity and increases endogenous @entity41579 levels. The caMEK6-dependent increase in gene expression is inhibited by the p38 MAPK inhibitor, @entity1441 , and is associated with a marked increase in @entity1435 MAPK activity; JNK and ERK kinases are not activated. In addition, @entity41579 gene expression is inhibited by dominant-negative @entity1435 and increased when caMEK6 and @entity1435 are co-expressed. caMEK6 also activates @entity10887 , but @entity10887 inhibits the caMEK6-dependent activation. These results suggest that @entity32027 increases @entity41579 gene expression by regulating the balance between activation of @entity1435 , which increases gene expression, and @entity10887 , which decreases gene expression.

@entity32027 regulates human @entity40330 gene expression via a @entity1435 - and XXXX -dependent mechanism.

@entity1441 :: ('MESH:C093642', 'Chemical') :: ['SB203580'] @entity7131 :: ('MESH:D020151', 'Disease') :: ['protein kinase C', 'PKC'] @entity44985 :: ('4214', 'Gene') :: ['MEKK1'] @entity32027 :: ('5608', 'Gene') :: ['MEK6'] @entity1435 :: ('1432', 'Gene') :: ['p38alpha'] @entity41579 :: ('89782', 'Gene') :: ['hINV'] @entity10887 :: ('5603', 'Gene') :: ['p38delta'] @entity40330 :: ('3713', 'Gene') :: ['involucrin']
@entity10887 :: (5603,Gene) :: ['p38delta']
