UNASSIGNED: Increased exposure to solar ultraviolet B (UVB) radiation causes oxidative damage that may promote @entity1020 ( @entity1020 ) and other ocular pathologies. This study is aimed to demonstrate the protective effects of some @entity4211 and xanthophylls against the UVB-induced oxidative damage to retinal pigment epithelial (RPE) cells. @entity1 cells were treated with 5  M @entity48672 , @entity70119 , @entity8665 , @entity35431 or a mixture of @entity48672   :   @entity35431 prior to UVB exposure (500 J m(-2)). Cell viability and mitogen-activated protein kinase (MAPK) phosphorylation were determined by @entity98 assay and western blot analysis, respectively. Oxidative damage was evaluated by measuring the intracellular reactive @entity26 species (ROS). The data showed that UVB irradiation reduces the cell viability to 46% with increasing of intracellular ROS levels and phosphorylation of MAPKs. However, pre-treatment (60 min) with 5  M @entity48672 , @entity8665 or @entity35431 significantly reduced cellular ROS levels and phosphorylation of MAPKs ( @entity10886 and @entity1435 ) mediated by UVB irradiation and subsequently increased cell viability. Thus, results show that UVB irradiation is able to induce apoptosis in @entity1 cells through oxidative stress; however @entity4211 and xanthophylls pre-treatment can attenuate this damage. This suggests that @entity48672 , @entity8665 and @entity35431 are effective in preventing UVB-induced damage in RPE cells and may be suitable as chemoprotective factors for the prevention of @entity16 . The use of natural dietary antioxidants might reduce ocular oxidative damage caused by UVB radiation.

A protective effect of XXXX and xanthophylls on UVB-induced damage in retinal pigment epithelial cells.

@entity1 :: ('9606', 'Species') :: ['ARPE-19'] @entity70119 :: ('CHEBI:31463', 'Chemical') :: ['delphinidin-3-O-glucoside'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity16 :: ('MESH:D009422', 'Disease') :: ['ocular damage'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity35431 :: ('MESH:D065146', 'Chemical') :: ['zeaxanthin'] @entity48672 :: ('MESH:C114438', 'Chemical') :: ['cyanidin-3-O-glucoside'] @entity4211 :: ('MESH:D000872', 'Chemical') :: ['anthocyanins'] @entity1435 :: ('1432', 'Gene') :: ['p38'] @entity1020 :: ('MESH:D008268', 'Disease') :: ['AMD', 'age related macular degeneration'] @entity10886 :: ('5599;5601', 'Gene') :: ['JNK1/2'] @entity8665 :: ('MESH:D014975', 'Chemical') :: ['lutein']
@entity4211 :: (MESH:D000872,Chemical) :: ['anthocyanins']
