The aim of the present work was to evaluate the number of apoptotic eosinophils in the livers of @entity742 experimentally infected with @entity8072 during the @entity1125 . Four groups (n=5) of @entity742 were used; groups 1-3 were orally infected with 200 metacercariae (mc) and sacrificed at 8 and 28 days post-infection (dpi), and 17 weeks post-infection (wpi), respectively. Group 4 was used as an uninfected control. Apoptosis was detected using immunohistochemistry with a polyclonal antibody against anti-active @entity40794 , and transmission electron microscopy (TEM). Eosinophils were identified using the Hansel stain in serial sections for @entity40794 , and by ultrastructural features using TEM. At 8 and 28 dpi, numerous @entity40794 (+) eosinophils were mainly found at the periphery of acute hepatic @entity39 foci. The percentage of @entity40794 (+) apoptotic eosinophils in the periphery of @entity39 foci was high (46.1-53.9) at 8 and 28 dpi, respectively, and decreased in @entity738 found at 28 dpi (6%). Transmission electron microscopy confirmed the presence of apoptotic eosinophils in @entity99 at 8 and 28 dpi. At 17 wpi, apoptotic eosinophils were detected in the infiltrate surrounding some enlarged bile ducts containing adult flukes. This is the first report of apoptosis induced by @entity8072 in @entity742 and the first study reporting apoptosis in eosinophils in hepatic inflammatory infiltrates in vivo. The high number of apoptotic eosinophils in acute @entity39 tracts during the @entity1125 suggests that eosinophil apoptosis may play a role in @entity8072 survival during different stages of infection.

@entity8072 induces eosinophil apoptosis in the XXXX in @entity742 .

@entity742 :: ('9940', 'Species') :: ['sheep'] @entity738 :: ('MESH:D006099', 'Disease') :: ['granulomas'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrotic'] @entity8072 :: ('6192', 'Species') :: ['Fasciola hepatica', 'F. hepatica'] @entity40794 :: ('443031(Tax:9940)', 'Gene') :: ['caspase -3', 'caspase-3'] @entity99 :: ('MESH:D056486', 'Disease') :: ['hepatic lesions'] @entity1125 :: ('MESH:D001661', 'Disease') :: ['migratory and biliary stages of infection']
@entity1125 :: (MESH:D001661,Disease) :: ['migratory and biliary stages of infection']
