@entity15066 is known to play important roles in both @entity342 -induced cell differentiation and inhibition of @entity263 @entity3 . However, the proteins associated with @entity15066 function are not fully enumerated. Using coimmunoprecipitation and mass spectrometry analysis, we identified 58 proteins that interact with @entity15066 in @entity263 cells. These proteins include nuclear proteins, adhesion molecules, endoplasmic reticulum (ER) chaperons, proteasome subunits, and signaling proteins. Integration of our data with protein-protein interaction data from the @entity1 Proteome Reference Database allowed us to build a comprehensive interactome map of @entity15066 . This interactome map consists of several modules such as a nuclear module and a cell membrane module; these modules explain the reported versatile functions of @entity15066 . We also determined that @entity563 330 and @entity1889 366 of @entity15066 were phosphorylated and demonstrated that the phosphorylation of @entity15066 was prominently mediated by protein kinase A (PKA). Further, we showed that @entity15066 directly binds to @entity3546 and @entity5962 . However, the phosphorylation of @entity15066 did not interrupt the binding of @entity15066 to @entity5962 and @entity3546 . Finally, we showed that the inhibition of @entity15066 expression by RNA interference decreased the ER inducible chaperon @entity19892 expression, directly proving that @entity15066 is involved in the ER stress response. Intriguingly, we observed that many members of the @entity15066 interactome are @entity342 -regulated and that the @entity15066 interactome links to the @entity342 response network through common interactions with @entity3546 and @entity1457 protein 90. Therefore we overlaid the transcriptomic expression changes in the @entity15066 interactome in response to @entity342 treatment and built a dual dynamic picture of the @entity15066 interactome in response to @entity342 . This interactome map provides the first road map for understanding the functions of @entity15066 in cells and its roles in @entity1 diseases, such as @entity263 , which can progress from @entity342 -dependent curable stages to @entity342 -independent incurable stages.

Proteomics analysis of the interactome of N-myc downstream regulated gene 1 and its interactions with the XXXX response program in @entity263 cells.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity263 :: ('MESH:D011471', 'Disease') :: ['prostate cancer'] @entity19892 :: ('7184', 'Gene') :: ['GRP94'] @entity1457 :: ('MESH:D012769', 'Disease') :: ['heat shock'] @entity5962 :: ('999', 'Gene') :: ['E-cadherin'] @entity3546 :: ('1499', 'Gene') :: ['beta-catenin'] @entity342 :: ('CHEBI:50113', 'Chemical') :: ['androgen'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity563 :: ('CHEBI:17822', 'Chemical') :: ['serine'] @entity1889 :: ('CHEBI:26986', 'Chemical') :: ['threonine'] @entity15066 :: ('10397', 'Gene') :: ['NDRG1']
@entity342 :: (CHEBI:50113,Chemical) :: ['androgen']
