PURPOSE: We examined the wound-healing effect of @entity6247 (VApal) on @entity19453 gene and protein expressions in a @entity35 @entity936 model based on lacrimal gland (LG) resection after injury. METHODS: The @entity35 @entity936 model was prepared by surgical resection of the main LG in male Long-Evans @entity35 . After alkaline injury of the central part of the lower palpebral conjunctiva bilaterally, VApal eye drops at 1,500   IU/mL in one eye and a vehicle in the fellow eye were both administered 6 times a day for 7 days. The expression of @entity19453 gene and protein was analyzed by real-time polymerase chain reaction or enzyme-linked immunosorbent assay in the @entity267 and conjunctiva of @entity41055 , @entity30816 , @entity68811 , and @entity7941 after 1, 3, (5), and 7 days of treatment with VApal. RESULTS: Significant decreases in @entity941 -stained areas and rose bengal scores were observed in @entity936 compared with vehicle-treated @entity936 at both 3 (P   <   0.05) and 7 days (P   <   0.01). Significant increases in corneal @entity30816 and conjunctival rMuc5AC after 1 day (P   <   0.01) and conjunctival @entity68811 gene expression after 3 days were observed with VApal treatment (P   <   0.05). Furthermore, conjunctival @entity68811 expression significantly increased after 3 days of VApal treatment (P   <   0.05). CONCLUSIONS: VApal promoted corneal @entity30816 , conjunctival rMuc5AC, and conjunctival @entity68811 gene expression in a @entity35 @entity936 model after injury. VApal also promoted conjunctival @entity68811 expression. These results indicate that VApal has efficacy in improving keratoconjunctival epithelial damage associated with decreased tear production.

Effect of Retinol Palmitate on Corneal and Conjunctival XXXX Gene Expression in a @entity35 Dry Eye Model After Injury.

@entity6247 :: ('MESH:C014794', 'Chemical') :: ['retinol palmitate'] @entity267 :: ('MESH:D065306', 'Disease') :: ['cornea'] @entity19453 :: ('65202(Tax:10116)', 'Gene') :: ['mucin'] @entity41055 :: ('24571(Tax:10116)', 'Gene') :: ['MUC1'] @entity30816 :: ('303887(Tax:10116)', 'Gene') :: ['MUC4', 'rMuc4'] @entity936 :: ('MESH:D015352', 'Disease') :: ['dry eye', 'dry eyes', 'VApal-treated dry eyes'] @entity7941 :: ('682837(Tax:10116)', 'Gene') :: ['MUC5AC'] @entity941 :: ('MESH:D019793', 'Chemical') :: ['fluorescein'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity68811 :: ('315451(Tax:10116)', 'Gene') :: ['rMuc16', 'MUC16']
@entity19453 :: (65202(Tax:10116),Gene) :: ['Mucin']
