UNASSIGNED: The existence of @entity356 stem cells (CSCs) has not been definitively proven and specific cell surface markers for identifying gastric CSCs have largely not been identified. Our research aimed to isolate potential gastric CSCs and clarify their clinical significance, while defining markers for GCSC identification and verification. Here, we report that spheroid cells possess stem cell-like properties, and overexpress certain stem cell markers. @entity5439 or @entity1759 -positive cells also exhibit properties of CSCs. The expression of @entity4729 , @entity4731 , @entity8889 , @entity1759 , @entity5439 , p-AKT, and @entity7952 was significantly higher in metastatic lesions compared to that in primary lesions. Elevated expression of some of these proteins was correlated with a more @entity146 phenotype and poorer prognosis, including @entity4729 , @entity4731 , @entity8889 , @entity1759 , and @entity7952 . Multivariate Cox proportional hazards model analysis showed that only @entity1759 is an independent factor. Knockdown of @entity1759 down-regulated the stem cell-like properties, which was accompanied by the down-regulation of p- @entity3963 and @entity4729 . @entity4729 overexpression could reverse the decreased CSCs properties induced by @entity1759 knockdown. Taken together, our research revealed that spheroid cell culture, and @entity5439 or @entity1759 -labeled FACS methods can be used to isolate gastric CSCs. Some CSC markers have clinical significance in predicting the prognosis. @entity1759 is an independent prognostic factor and maintains the properties of CSCs in @entity1759 -p- @entity3963 - @entity4729 positive feedback loop.

Identification of stem-like cells and clinical significance of candidate stem cell markers in XXXX .

@entity4729 :: ('5460', 'Gene') :: ['Oct4'] @entity5439 :: ('8842', 'Gene') :: ['CD133'] @entity146 :: ('MESH:D001523', 'Disease') :: ['aggressive'] @entity3963 :: ('5594', 'Gene') :: ['ERK'] @entity1759 :: ('960', 'Gene') :: ['CD44'] @entity4731 :: ('6657', 'Gene') :: ['Sox2'] @entity8889 :: ('2735', 'Gene') :: ['Gli1'] @entity7952 :: ('9451', 'Gene') :: ['p-ERK'] @entity356 :: ('MESH:D013274', 'Disease') :: ['gastric cancer']
@entity356 :: (MESH:D013274,Disease) :: ['gastric cancer']
