AIMS/HYPOTHESIS: Sympathetic inputs inhibit insulin secretion through alpha2-adrenergic receptors coupled with Gi protein. High adrenergic tonus generated by exposure of homeothermic animals to cold reduces insulin secretion. In this study we evaluate the participation of @entity5484 in cold-induced regulation of insulin secretion. METHODS: Static insulin secretion studies, western blotting and immunohistochemistry were used in this investigation. RESULTS: Exposure of @entity35 to cold during 8 days promoted 60% ( n=15, p<0.05) reduction of basal serum insulin levels concentration accompanied by reduction of the area under insulin curve during i.p. GTT (50%, n=15, p<0.05). Isolated islets from cold-exposed @entity35 secreted 57% ( n=6, p<0.05) less insulin following a @entity413 challenge. Previous sympathectomy, partially prevented the effect of cold exposure upon insulin secretion. Islets isolated from cold-exposed @entity35 expressed 51% ( n=6, p<0.5) more @entity5484 than islets from control @entity35 , while the inhibition of @entity5484 expression by antisense oligonucleotide treatment partially restored insulin secretion of islets obtained from cold-exposed @entity35 . Cold exposure also induced an increase of 69% ( n=6, p<0.05) in PGC-1 protein content in pancreatic islets. Inhibition of islet PGC-1 expression by antisense oligonucleotide abrogated cold-induced @entity5484 expression and partially restored insulin secretion in islets exposed to cold. CONCLUSION/INTERPRETATION: Our data indicate that sympathetic tonus generated by exposure of @entity35 to cold induces the expression of PGC-1, which participates in the control of @entity5484 expression in pancreatic islets. Increased @entity5484 expression under these conditions could reduce the beta-cell ATP/ @entity857 ratio and negatively regulate insulin secretion.

Peroxisome proliferator-activated receptor gamma coactivator-1-dependent uncoupling protein-2 expression in pancreatic islets of XXXX : a novel pathway for neural control of insulin secretion.

@entity35 :: ('10116', 'Species') :: ['rats'] @entity857 :: ('MESH:D000244', 'Chemical') :: ['ADP'] @entity5484 :: ('54315(Tax:10116)', 'Gene') :: ['UCP-2'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity35 :: (10116,Species) :: ['rats']
