UNASSIGNED: Microarray platforms, enabling simultaneous measurement of many allergens with a small serum sample are potentially powerful tools in @entity566 diagnostics. We here report the first study comparing a fully automated microarray system, Microtest Allergy System, with a manual microarray platform, ISAC, and two well-established singleplex @entity566 tests, skin prick test and ImmunoCAP, all tested on the same @entity1 . One hundred and three (103) adult @entity56 @entity1 attending the @entity566 clinic were included in the study. All @entity1 were tested with four @entity566 test methods (SPT, ImmunoCAP, Microtest and ISAC 112) and a total of 3485 pairwise test results were analysed and compared. The four methods showed comparable results with a positive/negative agreement of 81 - 88% for any pair of test methods compared, which is in line with data in the literature. The most prevalent allergens (cat, @entity235 , mite, timothy, birch and peanut) and their individual allergen components revealed an agreement between methods with correlation coefficients between 0.73 - 0.95. All four methods revealed deviating individual @entity1 results for a minority of @entity1 . These results indicate that microarray platforms are efficient and useful tools to characterize the specific IgE profile of @entity56 @entity1 using a small volume of serum sample. The results produced by the Microtest system were in agreement with diagnostic tests in current use. Further data collection and evaluation are needed for other populations, geographical regions and allergens. This article is protected by copyright. All rights reserved.

Evaluation of a novel automated @entity566 microarray platform compared with three other XXXX test methods.

@entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity566 :: ('MESH:D006967', 'Disease') :: ['allergy'] @entity56 :: ('MESH:D004342', 'Disease') :: ['allergic'] @entity235 :: ('9615', 'Species') :: ['dog']
@entity566 :: (MESH:D006967,Disease) :: ['allergy']
