BACKGROUND: Persistent infection %by with high risk @entity1082 (hrHPV) types causes cervical @entity5 but most @entity1 who test positive are at very low risk of @entity5 . Strategies are needed which can retain high sensitivity of hrHPV testing but reduce the number of false-positives. We showed previously that a combination DNA methylation triage assay for HPV types 16, 18 and 31 and @entity1 gene @entity20912 was useful to identify high grade @entity75 . OBJECTIVE: Assess whether measurement of DNA methylation in HPV type 33 can improve the previous classifier. METHODS: A London colposcopy referral group of 1493 @entity1 of whom 556 (37%) had histologically-confirmed @entity3146 ( @entity3146 ) 2 or 3 that included 114 HPV33 positive @entity1 with methylation measured for three L2 CpGs 5557, 5560 and 5566. Discrimination performance was assessed for the new classifier S5, built by adding HPV33 to the earlier classifier. RESULTS: HPV33 methylation measurement improved prediction among HPV33 positive @entity1 . Receiver operating characteristic analyses showed an area under the curve (AUC) for HPV33 methylation of 0.68 (95% CI 0.57-0.78). The earlier risk score was significantly improved by HPV33 methytlation (AUC = 0.82 vs 0.80; P < 0.001). For 90% sensitivity the specificity for CIN2/3 was 49% (95% CI 46-52%). CONCLUSIONS: Measurement of HPV33 DNA methylation contributes independent diagnostic information to @entity20912 and @entity1799 , HPV18 and HPV31, and is superior to genotyping. Other HPV and @entity1 methylation target regions might be useful to further improve S5.

HPV33 DNA methylation measurement improves cervical pre- @entity5 risk estimation of an @entity1799 , HPV18, HPV31 and \textit{ XXXX } methylation classifier.

@entity1 :: ('9606', 'Species') :: ['women', 'human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer', 'neoplasia'] @entity75 :: ('MESH:D001927', 'Disease') :: ['cervical lesions'] @entity20912 :: ('23136', 'Gene') :: ['EPB41L3'] @entity1799 :: ('333760', 'Species') :: ['HPV16'] @entity1082 :: ('10566', 'Species') :: ['human papillomavirus'] @entity3146 :: ('MESH:D018290', 'Disease') :: ['cervical intraepithelial neoplasia', 'CIN']
@entity20912 :: (23136,Gene) :: ['EPB41L3']
