@entity17577 is the major cause of posterior uvetis in European populations. The clinical diagnosis of toxoplasmic @entity6519 is based upon ophthalmoscopic findings, which are often but not always typical. Laboratory testing is therefore important to confirm the etiology of the disease. In the present 2-year prospective study, the relative diagnostic sensitivities of the three analytical techniques (enzyme-linked immunosorbent assay [ELISA], immunoblotting, and PCR) were compared by using a group of @entity1 (n = 19) with suspected @entity17577 . The relative specificities of the three techniques were assessed by including two control groups of @entity1 : one with nontoxoplasmic and noninflammatory @entity2194 (n = 48) and the other with nontoxoplasmic and inflammatory @entity2194 (n = 20). All 19 of the clinically suspect @entity1 had serological evidence of exposure to @entity3204 : 17 had been previously infected, and 2 had current @entity281 . The analysis of paired @entity7462 and serum samples by ELISA and immunoblotting revealed the local production of specific antibodies of the immunoglobulin G type in 63% (12 of 19) and 53% (10 of 19) of @entity1 , respectively. PCR analysis of @entity7462 samples confirmed the presence of @entity3204 DNA in 28% (5 of 18) of cases. When combined, ELISA, immunoblotting, and PCR findings confirmed the toxoplasmic origin of retinal lesions in 83% (15 of 18) of @entity1 . The relative specificities of the three techniques were 89% for ELISA and immunoblotting and 100% for PCR.

Comparison of enzyme-linked immunosorbent assay, immunoblotting, and PCR for diagnosis of toxoplasmic XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity2194 :: ('MESH:D005128', 'Disease') :: ['ocular disease'] @entity7462 :: ('MESH:C562390', 'Disease') :: ['aqueous humor'] @entity17577 :: ('MESH:D014126', 'Disease') :: ['Ocular toxoplasmosis', 'ocular toxoplasmosis'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity6519 :: ('MESH:D002825', 'Disease') :: ['chorioretinitis'] @entity3204 :: ('5811', 'Species') :: ['T. gondii', 'Toxoplasma gondii']
@entity6519 :: (MESH:D002825,Disease) :: ['chorioretinitis']
