UNASSIGNED: The aim of this study was to evaluate the effect of a new anti-fibrotic agent, @entity3131 ( @entity15795 ), delivered using polyelectrolyte multilayer films on excisional wound healing. Polyelectrolyte multilayer films were prepared by layer-by-layer (LbL) sequential adsorption of chitosan and @entity14917 . The UV-spectrophotometer, FTIR and differential scanning calorimeter were used to characterize the LbL thin films. The @entity15795 was entrapped within the LbL thin films and its effect on excisional wound healing was studied in C57BL/6. The total protein, collagen content and @entity641 expression within the wound tissue were determined after application of @entity15795 using LbL thin films, chitosan hydrogel and @entity968 hydrogel. UV-spectrophotometer and FTIR studies showed a sequential adsorption of chitosan and alginate polymer layers to form LbL thin films. The thickness of LbL thin films with 15 bilayers was found to be 15  2 m. HPLC analysis showed a @entity15795 loading efficiency of 1.0  0.1mg in 1cm(2) area of LbL thin film. In vivo wound healing studies in C57BL/6 @entity19 showed an accelerated (<9days) wound contraction after treatment with the @entity15795 compared with blank LbL thin film and commercial @entity4279 gel (12days). The collagen content within the wound tissue was significantly (p<0.05) less after treatment with @entity15795 compared with blank film application. Western blot analysis showed gradual decrease in @entity641 expression within the wound tissue after treatment with @entity15795 . This study for the first time demonstrated that new anti-fibrotic agent @entity15795 loaded in LbL thin films can be utilized for excisional wound healing.

Effect of XXXX delivered using layer-by-layer thin film on excisional wound healing.

@entity15795 :: ('CHEBI:31263', 'Chemical') :: ['PFD'] @entity641 :: ('21803(Tax:10090)', 'Gene') :: ['TGF-b'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity3131 :: ('MESH:C093844', 'Chemical') :: ['pirfenidone'] @entity4279 :: ('MESH:D011206', 'Chemical') :: ['povidone-iodine'] @entity14917 :: ('MESH:C008970', 'Chemical') :: ['sodium alginate'] @entity968 :: ('MESH:D011092', 'Chemical') :: ['polyethylene glycol']
@entity3131 :: (MESH:C093844,Chemical) :: ['pirfenidone']
