Some strains of @entity526 related to acute @entity2335 or @entity1960 produce a toxin named cytotoxic necrotizing factor 1 (CNF-1). CNF-1 mediates its effects on epithelial cells or phagocytes via the permanent activation of small GTP-binding proteins, caused by the toxin-induced deamidation of Glu(63) of p21 Rho. The behavior of peripheral blood T lymphocytes during the acute phase of @entity1960 has been poorly investigated. Our study was conducted to test whether (i) peripheral blood T lymphocytes can be activated by CNF-1 and (ii) CNF-1-activated T lymphocytes are cytotoxic against intestinal epithelial cells. Activation of T lymphocytes by CNF-1 was assessed by electrophoresis, flow cytometry, confocal microscopy, and electron microscopy studies. Assays for migration and adherence of CNF-1-treated T lymphocytes were performed in Transwell chambers with T84 intestinal epithelial cells grown on @entity6027 semipermeable filters. CNF-1 induced a decrease in the electrophoretic mobility of the GTP-binding protein Rho in treated T lymphocytes. CNF-1 provoked an increase in the content of actin stress fibers and pseudopodia in T lymphocytes. Several adherence molecules were clustered into cytoplasmic projections in CNF-1-treated T lymphocytes and adherence of such lymphocytes on the basolateral pole of T84 was increased, resulting in @entity137 toward epithelial cells. Such enhanced adherence in response to CNF-1 was dependent on p42-44(MAP) kinase activation of T lymphocytes. Taken together, these results suggest that CNF-1, by acting on T lymphocytes, may increase in an important fashion the virulence of certain strains of @entity526 against the intestinal epithelia.

Rho GTPase is activated by cytotoxic necrotizing factor 1 in peripheral blood T lymphocytes: potential XXXX for intestinal epithelial cells.

@entity6027 :: ('CHEBI:61433', 'Chemical') :: ['polycarbonate'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity1960 :: ('MESH:D003092', 'Disease') :: ['colitis', 'bacterial colitis'] @entity2335 :: ('MESH:D003556', 'Disease') :: ['cystitis'] @entity526 :: ('562', 'Species') :: ['Escherichia coli', 'E. coli']
@entity137 :: (MESH:D064420,Disease) :: ['cytotoxicity']
