BACKGROUND: Ciliary beating by respiratory epithelial cells continuously purges pathogens from the lower airways. Here we investigated the effect of the fungal cell wall polysaccharides Galactomannan (GM) and Zymosan (Zym) on the adrenergic activated particle transport velocity ( @entity343 ) of tracheal epithelium. METHODS: Experiments were performed using tracheae isolated from male C57BL/6J @entity19 . Transport velocity of the cilia bearing epithelial cells was measured by analysing recorded image sequences. Generation of reactive @entity26 species (ROS) were determined using Amplex Red reagents. PCR experiments were performed on isolated tracheal epithelium to identify adrenergic receptor mRNA. RESULTS: The adrenergic receptors a1D, a2A, @entity8872 have been identified in isolated tracheal epithelium. We found @entity1425 responsible for an increase in @entity343 , which could only be reduced by selective b-receptor-inhibition. In addition, either GM or Zym prevented the @entity1425 induced @entity343 increase. Furthermore, we observed a strong ROS generation evoked by GM or Zym. However, @entity1425 induced increase in @entity343 recovered in the presence of GM and Zym after application of ROS scavengers. CONCLUSION: Both GM or Zym trigger reversible ROS generation in tracheal tissue leading to inhibition of the b-adrenergic increase in @entity343 .

Galactomannan and Zymosan Block the XXXX -Induced Particle Transport in Tracheal Epithelium.

@entity19 :: ('10090', 'Species') :: ['mice'] @entity1425 :: ('MESH:D004837', 'Chemical') :: ['epinephrine'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity343 :: ('187978', 'Species') :: ['PTV'] @entity8872 :: ('28905;3383', 'Gene') :: ['b1 and b2']
@entity1425 :: (MESH:D004837,Chemical) :: ['Epinephrine']
