BACKGROUND: Arctium lappa (Niubang), a Chinese herbal medicine, is used to treat tissue @entity32 . This study investigates the effects of @entity24738 (AC), isolated from A. lappa, on anti-CD3/ @entity1361 Ab-stimulated cell proliferation and cytokine gene expression in primary @entity1 T lymphocytes. METHODS: Cell proliferation was determined with enzyme immunoassays and the tritiated @entity1014 uptake method. Cytokine production and gene expression were analyzed with reverse transcription-polymerase chain reaction. RESULTS: AC inhibited primary @entity1 T lymphocytes proliferation activated by anti-CD3/ @entity1361 Ab. Cell viability test indicated that the inhibitory effects of AC on primary @entity1 T lymphocyte proliferation were not due to direct @entity137 . AC suppressed @entity1199 ( @entity1199 ) and @entity379 ( @entity379 ) production in a concentration-dependent manner. Furthermore, AC decreased the @entity1199 and @entity379 gene expression in primary @entity1 T lymphocytes induced by anti-CD3/ @entity1361 Ab. Reporter gene analyses revealed that AC decreased NF-AT-mediated reporter gene expression. CONCLUSION: AC inhibited T lymphocyte proliferation and decreased the gene expression of @entity1199 , @entity379 and NF-AT.

@entity24738 from Arctium lappa inhibits @entity1199 and interferon gene expression in primary XXXX T lymphocytes.

@entity1 :: ('9606', 'Species') :: ['human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity24738 :: ('MESH:C071942', 'Chemical') :: ['arctigenin'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity379 :: ('3458', 'Gene') :: ['interferon-y', 'IFN-y'] @entity1014 :: ('MESH:D013936', 'Chemical') :: ['thymidine'] @entity1199 :: ('3558', 'Gene') :: ['interleukin-2', 'IL-2'] @entity1361 :: ('940', 'Gene') :: ['CD28']
@entity1 :: (9606,Species) :: ['human']
