The aerobic @entity375 @entity11900 and the predominantly fermentative @entity375 share many of the genes encoding the enzymes of @entity505 and energy metabolism. The physiological features that distinguish the two @entity375 appear to result essentially from different organization of regulatory circuits, in particular @entity413 repression and gluconeogenesis. We have isolated the KlCAT8 gene (a homologue of @entity375 @entity15518 , encoding a DNA binding protein) as a multicopy suppressor of a fog1 mutation. The Fog1 protein is a homologue of the Snf1 complex components @entity69989 , @entity69990 , and @entity69991 of @entity375 . While @entity15518 controls the key enzymes of gluconeogenesis in @entity375 , KlCAT8 of @entity11900 does not (I. Georis, J. J. Krijger, K. D. Breunig, and J. Vandenhaute, Mol. Gen. Genet. 264:193-203, 2000). We therefore examined possible targets of KlCat8p. We found that the @entity16374 coenzyme A synthetase genes, KlACS1 and KlACS2, were specifically regulated by KlCAT8, but very differently from the @entity375 counterparts. KlACS1 was induced by @entity598 and @entity880 , while KlACS2 was induced by @entity1068 , both under the control of KlCAT8. Also, KlJEN1, encoding the @entity880 -inducible and @entity413 -repressible @entity880 permease, was found under a tight control of KlCAT8.

Three target genes for the transcriptional activator Cat8p of @entity11900 : @entity16374 coenzyme A synthetase genes KlACS1 and KlACS2 and XXXX permease gene KlJEN1.

@entity15518 :: ('855323(Tax:4932)', 'Gene') :: ['CAT8'] @entity69989 :: ('856749(Tax:4932)', 'Gene') :: ['Gal83p'] @entity16374 :: ('CHEBI:40574', 'Chemical') :: ['acetyl'] @entity598 :: ('CHEBI:47622', 'Chemical') :: ['acetate'] @entity505 :: ('MESH:D002244', 'Chemical') :: ['carbon'] @entity69990 :: ('852032(Tax:4932)', 'Gene') :: ['Sip1p'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity11900 :: ('28985', 'Species') :: ['Kluyveromyces lactis', 'K. lactis'] @entity69991 :: ('852664(Tax:4932)', 'Gene') :: ['Sip2p'] @entity375 :: ('4932', 'Species') :: ['yeast', 'Saccharomyces cerevisiae', 'yeasts', 'S. cerevisiae'] @entity1068 :: ('MESH:D000431', 'Chemical') :: ['ethanol'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity880 :: (MESH:D019344,Chemical) :: ['lactate']
