OBJECTIVE: An isolate of @entity375 with reduced susceptibility to @entity2380 and @entity2059 was developed in the laboratory and used to identify genes that are differentially expressed in association with this phenotype. METHODS: @entity375 ATCC 9763 was passaged in increasing concentrations of @entity2059 . @entity2059 and @entity2380 MICs for the initial isolate (9763S) were 2 and 16 mg/L and for the final isolate (9763I) were 16 and > or =64 mg/L, respectively. Duplicate sets of total RNA from 9763S and 9763I were isolated and hybridized to Affymetrix S98 @entity375 arrays. To validate results, six differentially expressed genes were further examined by RT-PCR. RESULTS: Of the nearly 6400 open reading frames represented on the array, a total of 116 genes (1.8%) were found to be differentially expressed. Cell wall maintenance genes @entity69104 and CCW12, @entity5068 metabolism gene @entity41224 , small molecule transport genes @entity69105 and @entity69106 , and stress response gene @entity12091 were expressed at a level at least 2.5-fold higher than the expression level found in 9763S. Eleven energy generation genes, ionic homeostasis genes @entity69107 , @entity69108 and @entity69109 , and @entity5068 metabolism genes @entity69110 and ERG13 were expressed at least 2.5-fold lower than the expression level found in 9763S. CONCLUSIONS: Several genes found to be differentially expressed in this study have been shown previously to be differentially expressed in the fungal response to @entity5638 treatment. In addition, the potential role of @entity69105 and/or @entity69106 as mediators of drug efflux is intriguing and warrants further study.

Identification of genes differentially expressed in association with reduced azole susceptibility in XXXX .

@entity69108 :: ('853660(Tax:4932)', 'Gene') :: ['FRE2'] @entity2380 :: ('MESH:D015725', 'Chemical') :: ['fluconazole'] @entity12091 :: ('856450(Tax:4932)', 'Gene') :: ['CUP1-1'] @entity69106 :: ('856444(Tax:4932)', 'Gene') :: ['YHK8'] @entity41224 :: ('851799(Tax:4932)', 'Gene') :: ['UPC2'] @entity69107 :: ('850911(Tax:4932)', 'Gene') :: ['FRE1'] @entity69104 :: ('854173(Tax:4932)', 'Gene') :: ['TIR4'] @entity69109 :: ('855797(Tax:4932)', 'Gene') :: ['FRE4'] @entity5068 :: ('CHEBI:15889', 'Chemical') :: ['sterol'] @entity375 :: ('4932', 'Species') :: ['yeast', 'S. cerevisiae strain', 'S. cerevisiae'] @entity69105 :: ('854175(Tax:4932)', 'Gene') :: ['AUS1'] @entity5638 :: ('CHEBI:68452', 'Chemical') :: ['azole'] @entity2059 :: ('MESH:D017964', 'Chemical') :: ['Itraconazole', 'itraconazole'] @entity69110 :: ('855260(Tax:4932)', 'Gene') :: ['ERG8']
@entity375 :: (4932,Species) :: ['Saccharomyces cerevisiae']
