Mitochondrial membrane potential (delta psi(m)) was determined in intact isolated nerve terminals using the membrane potential-sensitive probe JC-1. Oxidative stress induced by @entity274 (0.1-1 mM) caused only a minor decrease in delta psi(m). When complex I of the respiratory chain was inhibited by @entity3788 (2 microM), delta psi(m) was unaltered, but on subsequent addition of @entity274 , delta psi(m) started to decrease and collapsed during incubation with 0.5 mM @entity274 for 12 min. The @entity855 level and [ @entity855 ]/[ @entity857 ] ratio were greatly reduced in the simultaneous presence of @entity3788 and @entity274 . @entity274 also induced a marked reduction in delta psi(m) when added after @entity4626 (10 microM), an inhibitor of @entity7257 . @entity274 (0.1 or 0.5 mM) inhibited @entity15706 dehydrogenase and decreased the steady-state @entity1110 level in nerve terminals. It is concluded that there are at least two factors that determine delta psi(m) in the presence of @entity274 : (a) The @entity2125 level reduced owing to inhibition of @entity15706 dehydrogenase is insufficient to ensure an optimal rate of respiration, which is reflected in a fall of delta psi(m) when the @entity7257 is not functional. (b) The greatly reduced @entity855 level in the presence of @entity3788 and @entity274 prevents maintenance of delta psi(m) by @entity7257 . The results indicate that to maintain delta psi(m) in the nerve terminal during @entity274 -induced oxidative stress, both complex I and @entity7257 must be functional. Collapse of delta psi(m) could be a critical event in @entity528 in @entity63 or @entity191 when @entity274 is generated in excess and complex I of the respiratory chain is simultaneously impaired.

Depolarization of in situ mitochondria due to hydrogen peroxide-induced oxidative stress in nerve terminals: inhibition of XXXX dehydrogenase.

@entity2125 :: ('MESH:D009243', 'Chemical') :: ['NADH'] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H2O2'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemia'] @entity15706 :: ('MESH:C029743', 'Chemical') :: ['alpha-ketoglutarate'] @entity3788 :: ('MESH:D012402', 'Chemical') :: ['rotenone'] @entity1110 :: ('MESH:D009249', 'Chemical') :: ['NAD(P)H'] @entity191 :: ('MESH:D010300', 'Disease') :: ["Parkinson's disease"] @entity7257 :: ('514', 'Gene') :: ['F0F1-ATPase'] @entity857 :: ('MESH:D000244', 'Chemical') :: ['ADP'] @entity528 :: ('MESH:D009410', 'Disease') :: ['neuronal injury'] @entity4626 :: ('MESH:D009840', 'Chemical') :: ['oligomycin'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity15706 :: (MESH:C029743,Chemical) :: ['alpha-ketoglutarate']
