BACKGROUND: Although several in vivo antigenicity assays using parenteral immunization are operational, no adequate enteral sensitization models are available to study @entity5419 and allergenicity of food proteins. OBJECTIVE: This paper describes the development of an enteral model for @entity5419 research in the Brown Norway (BN) @entity35 . METHODS: The animals were exposed to ovalbumin either ad libitum via the drinking water (0.002 to 20 mg/mL) continuously for 6 weeks or by gavage (1 mg/mL per @entity35 ). Gavage dosing was performed either daily, twice a week, once a week or once every 2 weeks during a period of 6 weeks. No adjuvants were used during the sensitization studies. RESULTS: After intra-gastric administration of ovalbumin once or twice a week or once every two weeks, no or only a very low frequency of ovalbumin-specific antibody responses were detected. Daily intra-gastric dosing with ovalbumin resulted in antigen-specific IgG as well as IgE responses in almost all animals tested. Upon ad libitum exposure, ovalbumin-specific IgG but no ovalbumin-specific IgE was detected. The cellular response was examined by determination of @entity56 ( @entity56 ) reactions in the animals dosed by daily gavage and in the ad libitum exposed @entity35 . Both sensitization protocols sensitized for @entity56 . The response was most pronounced in ad libitum exposed @entity35 at day 28 of exposure. CONCLUSIONS: These studies show that the BN @entity35 may provide a suitable animal model for inducing specific IgG and IgE responses as well as specific @entity56 ( @entity56 ) to ovalbumin upon exposure via the enteral route without the use of adjuvants.

Oral sensitization to food proteins: a Brown XXXX model.

@entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity56 :: ('MESH:D004342', 'Disease') :: ['DTH', 'delayed-type hypersensitivity', 'T-cell mediated hypersensitivity'] @entity5419 :: ('MESH:D005512', 'Disease') :: ['food allergy']
@entity35 :: (10116,Species) :: ['Norway rat']
