Metabolomics is the comprehensive assessment of endogenous metabolites of a biological system in a holistic context, and its property consists with the global view of @entity1058 ( @entity1058 ). Suanzaoren decoction (SZRD), an ancient @entity1058 formulae, has been used for treating @entity22 for centuries, and its mechanism remains unclear completely. This paper was designed to explore globally metabolomic characters of the @entity22 and the therapeutic effects of SZRD. Ultra-performance liquid-chromatography/electrospray-ionization synapt high-definition mass spectrometry (UPLC/ESI-SYNAPT-HDMS) combined with pattern recognition approaches including principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and orthogonal projection to latent structures discriminate analysis (OPLS-DA) were integrated to approximate the comprehensive metabolic signature and discover differentiating metabolites. The changes in metabolic profiling were restored to their baseline values after SZRD treatment according to the PCA score plots. Altogether, the current metabonomic approach based on UPLC/ESI-SYNAPT-HDMS indicate 20 ions (9 in the negative mode, 11 in the positive mode) as "differentiating metabolites". The alterations in these metabolites were associated with perturbations in @entity535 and @entity844 metabolism, in response to @entity22 through immune and nervous system. Of note, we found that SZRD increases sleep activity and exhibits binding affinity for @entity750 receptors. These results implicate the therapeutic effects of SZRD may mediate through serotonergic activation. Our findings also show the robust UPLC/ESI-SYNAPT-HDMS techniques is promising for metabolites profiling analysis of @entity1058 and open new perspectives to using metabolomics platform to resolve special @entity1058 issues.

Metabolomic study of XXXX and intervention effects of Suanzaoren decoction using ultra-performance liquid-chromatography/electrospray-ionization synapt high-definition mass spectrometry.

@entity844 :: ('CHEBI:35366', 'Chemical') :: ['fatty acid'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity750 :: ('MESH:D012701', 'Chemical') :: ['serotonin'] @entity1058 :: ('MESH:C562377', 'Disease') :: ['traditional Chinese medicine', 'TCM'] @entity22 :: ('MESH:D007319', 'Disease') :: ['insomnia']
@entity22 :: (MESH:D007319,Disease) :: ['insomnia']
