Scrapie was transmitted to @entity19 from ten @entity742 , collected in the UK between 1985 and 1994. As in previous natural scrapie transmissions, the results varied between scrapie sources in terms of the incidence of disease, incubation periods and neuropathology in challenged @entity19 . This contrasted with the uniformity seen in transmissions of @entity9567 to @entity19 . The scrapie and @entity9567 isolates were characterized further by serial passage in @entity19 . Different TSE strains were isolated from each source according to the Sinc or @entity65885 genotype of the @entity19 used for passage. The same two @entity19 -passaged strains, 301C and 301V, were isolated from each of three @entity9567 sources. Despite the variation seen in the primary transmissions of scrapie, relatively few @entity19 -passaged scrapie strains were isolated and these were distinct from the @entity9567 -derived strains. The ME7 @entity11506 , which has often been isolated from independent @entity742 sources in the past, was identified in isolates from four of the @entity742 . However, a new distinct strain, 221C, was derived from a further four scrapie @entity742 . These results suggest that there is agent strain variation in natural scrapie in @entity742 and that the spectrum of strains present may have changed over the last 20 years. The tested sample is too small to come to any conclusions about whether the @entity6475 is present in @entity742 , but the study provides a framework for further more extensive studies.

Strain characterization of natural @entity742 scrapie and comparison with XXXX .

@entity9567 :: ('MESH:D016643', 'Disease') :: ['BSE'] @entity742 :: ('9940', 'Species') :: ['sheep'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity65885 :: ('18814(Tax:10090)', 'Gene') :: ['PrP'] @entity6475 :: ('MESH:D013180', 'Disease') :: ['BSE strain'] @entity11506 :: ('MESH:D012608', 'Disease') :: ['scrapie strain']
@entity9567 :: (MESH:D016643,Disease) :: ['BSE']
