BACKGROUND: Immunophenotype is an essential parameter in the diagnosis of @entity1583 . @entity450 ( @entity450 ) is used in the analysis of bone marrow or peripheral blood samples but is less frequently used in the evaluation of tissue biopsies with suspected @entity1583 . The aim of this study was to analyze the role of @entity450 in the diagnosis of biopsies from @entity1 with a suspected @entity1503 . METHODS: A total of 422 consecutive biopsies were studied using standard morphology, immunohistochemistry (IHC), and @entity450 . Results of @entity450 were obtained in less than 3 h and were interpreted independently from morphology and IHC. RESULTS: A strong correlation between @entity5 and abnormal pattern of @entity450 was observed (218 of 250) with the exception of @entity978 (P < 0.001). Overall, negative predictive value was 0.52 and positive predictive value was 1. Light chain restriction was observed in 182 of 201 @entity1892 and in 0 of 142 non-B-cell disorders by @entity450 . In contrast, light chain pattern could only be evaluated in 38 of 91 cases by IHC. @entity450 allowed a rapid diagnosis of infrequent or high-grade @entity5 such as @entity2058 or @entity981 . The addition of @entity450 in the routine study of tissue biopsies facilitates the diagnosis of double pathology in five (1%) @entity1 . CONCLUSIONS: @entity450 is a fast and reliable methodology for phenotyping tissue samples, which easily detects infrequent @entity1583 , disease-specific phenotypes and clonality in @entity1892 . Moreover, the simultaneous recognition of different cell populations allows the @entity1892 , or double pathologies.

Routine use of immunophenotype by flow cytometry in tissues with suspected XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity981 :: ('MESH:D054198', 'Disease') :: ['T-lymphoblastic lymphoma'] @entity5 :: ('MESH:D009369', 'Disease') :: ['malignant disease', 'malignancies'] @entity2058 :: ('MESH:D054747', 'Disease') :: ['histiocytic sarcoma'] @entity450 :: ('MESH:D006940', 'Disease') :: ['Flow cytometry', 'FCM'] @entity1503 :: ('MESH:D006402', 'Disease') :: ['hematological disorder'] @entity1892 :: ('MESH:D016393', 'Disease') :: ['B-cell lymphomas', 'diagnosis of composite cell lymphomas', 'B-cell lymphoma'] @entity1583 :: ('MESH:D019337', 'Disease') :: ['hematological malignancies'] @entity978 :: ('MESH:D006689', 'Disease') :: ['Hodgkin disease']
@entity1583 :: (MESH:D019337,Disease) :: ['hematological malignancies']
