Neural transplantation as an experimental therapy for @entity191 @entity1 has been shown to be effective in several clinical trials. Further benefit, however, may be expected if the grafting is combined with a treatment of neurotrophic factors thus improving the survival and growth of grafted embryonic dopaminergic neurons. Continuous trophic support may be needed and therefore requires the long-term delivery of neurotrophic factors to the brain. We demonstrate here that the implantation of polymer-encapsulated cells genetically engineered to continuously secrete @entity10157 to the adult @entity35 striatum improves dopaminergic graft survival and function. Near complete compensation of @entity2955 -induced rotation was already achieved within 3 weeks postgrafting in @entity35 that received @entity10157 -releasing capsules in addition to dopaminergic cell grafts of cultured tissue. @entity35 without trophic factor supply showed only little recovery at the same time point and sham grafted @entity35 showed no recovery. The number of @entity669 hydroxylase-immunoreactive cells per graft was increased 2.6-fold in the presence of @entity10157 6 weeks postgrafting. Similarly, @entity669 hydroxylase-immunoreactive fibers around the graft were increased by 53%. Moreover, these fibers showed a preferential growth towards the trophic factor-releasing capsule. Taken together, these results provide evidence that encapsulated genetically engineered cells are an effective means of long-term trophic factor supply into the adult @entity35 brain and that the delivery of @entity10157 can sustain dopaminergic graft function and survival.

Implants of polymer-encapsulated genetically modified cells releasing XXXX improve survival, growth, and function of fetal dopaminergic grafts.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity35 :: ('10116', 'Species') :: ['rat', 'Rats', 'rats'] @entity191 :: ('MESH:D010300', 'Disease') :: ['Parkinsonian'] @entity10157 :: ('25453(Tax:10116)', 'Gene') :: ['glial cell line-derived neurotrophic factor'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine'] @entity2955 :: ('MESH:D016627', 'Chemical') :: ['6-hydroxydopamine']
@entity10157 :: (25453(Tax:10116),Gene) :: ['glial cell line-derived neurotrophic factor']
