A new cell line, designated UHKBR-01, was successfully established from a @entity3653 ( @entity106 )-induced @entity35 mammary @entity5 . @entity106 was administered orally at a dose of 4 mg/ml per @entity35 on the first day of the experiment and thereafter at weekly intervals of same dosage, until the @entity35 have reached a weight of around 150-200 g. The @entity5 grew rapidly after the injection, and were transplanted into @entity19 one the harvest size (2.5 x 2 x 1 mm(3)) was reached, it was transplanted onto @entity19 . We have developed a cell line from a portion of the @entity106 -induced @entity189 of the @entity19 . The UHKBR-01 cell exhibited a slow increase in growth rate during the time of culture and was highly tumourigenic in @entity19 . The cells have been grown in culture for over 40 passages. Characterization of the cell line was performed. This included morphology by light and transmission electron microscopy, karyotype, growth rate, @entity5 antigen expression and xenograft implantation into @entity19 . These cells exhibit ultrastructural and immunohistochemical features of epithelial cells of mammary origin. The above analyses also demonstrated that UHKBR-01 cells were @entity2931 - and @entity1420 -receptor positive, in likeness to other @entity0 cell lines such as MDA-MB-231 and MCF-7. The cell line grows as monolayers of oval-shaped cells with large folded nuclei accompanied by a rich supply of mitochondria. This report describes the first in vitro cell line from transplantable @entity106 -induced @entity189 of @entity19 , which presents unique characteristics that may prove to be a good experimental model for investigating @entity0 biology.

A XXXX cell line derived from @entity106 -induced @entity189 .

@entity3653 :: ('CHEBI:254496', 'Chemical') :: ['7,12-dimethylbenz[a]anthracene'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer', 'established breast cancer'] @entity1420 :: ('MESH:D011374', 'Chemical') :: ['progesterone'] @entity2931 :: ('CHEBI:50114', 'Chemical') :: ['oestrogen'] @entity19 :: ('10090', 'Species') :: ['nude mice'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumour', 'tumours'] @entity189 :: ('MESH:D002277', 'Disease') :: ['mammary carcinoma', 'carcinoma'] @entity106 :: ('MESH:D015127', 'Chemical') :: ['DMBA'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats']
@entity35 :: (10116,Species) :: ['rat']
