PURPOSE: We investigated whether @entity9381 injected in the bladder would affect preganglionic parasympathetic nerve endings in intramural ganglia. MATERIALS AND METHODS: @entity840 bladders were injected with 5 U of botulinum toxin. At 24 hours bladders were collected and processed for immunohistochemistry using @entity669 hydroxylase, and intact and cleaved @entity28124 . To identify the different populations of affected fibers coursing the ganglia we performed double immunoreactions for cleaved @entity28124 and VAChT, TH or CGRP. RESULTS: VAChT immunoreactive fibers were identified in axons and varicosities of presynaptic to postganglionic parasympathetic neurons. Those fibers were also immunoreactive to SV2 and @entity28124 . The rare CGRP and TH immunoreactive fibers coursing in the ganglia did not express SV2 or @entity28124 . After @entity9381 injection the cleaved form of @entity28124 was abundantly expressed in parasympathetic fibers. CONCLUSIONS: Botulinum toxin injection in the bladder wall affects preganglionic parasympathetic nerve terminals. This could contribute to the strong effect of botulinum toxin on bladder smooth muscle activity.

Effect of @entity9381 on intramural parasympathetic ganglia: an experimental study in the XXXX bladder.

@entity28124 :: ('100724783', 'Gene') :: ['SNAP-25'] @entity840 :: ('10141', 'Species') :: ['Guinea pig'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine'] @entity9381 :: ('MESH:C542870', 'Chemical') :: ['onabotulinumtoxinA']
@entity840 :: (10141,Species) :: ['guinea pig']
