The @entity2856 ( @entity2856 ) has been shown to mediate mitogenesis and suppression of apoptosis. Certain mutations in the @entity1473 terminus of the receptor abrogate the antiapoptotic activity but not the mitogenic activity. However, truncation of the receptor by deletion of the @entity1473 -terminal 108 @entity1522 enhances suppression of apoptosis by the @entity2856 , which suggests that the @entity1473 terminus has a negative regulatory role. To investigate this further, a series of @entity1 expression vectors were generated that encoded either the @entity1473 terminus of the receptor or the @entity1473 terminus plus the kinase domain. In some cases, the first 16 @entity1522 of SRC were included at the @entity2733 terminus to provide a site for myristylation. In transient transfection assays, the membrane-targeted @entity1473 -terminal construct, MyCF, was found to induce apoptosis in MCF-7 @entity0 cells and C6 @entity463 cells, whereas the @entity1473 -terminal construct without the myristylation signal, @entity1577 , was poorly cytotoxic. MyKCF, which encodes the kinase domain as well as the @entity1473 terminus, had intermediate @entity137 . The @entity137 of MyCF was diminished by point mutations that were previously shown to abrogate suppression of apoptosis in the context of the full-length receptor. MCF-7 cells stably expressing the @entity1577 or the MyCF proteins exhibited decreased clonogenicity in soft agar and increased sensitivity to UV irradiation. These results indicate that expression of the @entity2856 @entity1473 terminus promotes apoptosis of @entity5 cells, possibly by interfering with signals necessary for cell survival.

Expression of the @entity2856 C terminus as a myristylated protein leads to induction of apoptosis in XXXX cells.

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast carcinoma'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity2733 :: ('CHEBI:29345', 'Chemical') :: ['NH2'] @entity2856 :: ('3480', 'Gene') :: ['insulin-like growth factor I receptor', 'IGF-IR'] @entity463 :: ('MESH:D005909', 'Disease') :: ['glioblastoma'] @entity1577 :: ('MESH:D003550', 'Disease') :: ['CF'] @entity1473 :: ('CHEBI:46883', 'Chemical') :: ['COOH']
@entity5 :: (MESH:D009369,Disease) :: ['tumor']
