A protein encoded by At1g32080 was consistently identified in proteomic studies of Arab @entity958 oroplast envelope membranes, but its function remained unclear. The protein, designated AtLrgB, may have evolved from a gene fusion of lrgA and lrgB. In bacteria, two homologous operons, lrgAB and cidAB, participate in an emerging mechanism to control cell deat @entity204 lysis.     We aim to characterize AtLrgB using reverse genetics and cell biological and biochemical analysis.     AtLrgB is expressed in leaves, but not in roots. T-DNA insertion mutation of AtLrgB produced plants with interveinal chlorotic and premature necrotic leaves. Overexpression of full-length AtLrgB (or its LrgA and LrgB domains, separately), under the control of CaMV @entity3260 promoter, produced plants exhibiting vein @entity24194 delayed greening. At the end of light period, the T-DNA mutant had high starch and low sucr @entity969 tents in leaves, while the 35S:AtLrgB plants had low starch and high sucr @entity969 tents. Metabolite profiling revealed that AtLrgB appeared not to directly transport triose phos @entity1391 hexo @entity20251 yeas @entity375 ls, AtLrgB could augment nyst @entity8701 uced membrane permeability.     Our work indicates that AtLrgB is a new player in chloroplast development, carb @entity505 titioning and leaf senescence, although its molecular mechanism remains to be established.

A chloroplast envelope membrane protein containing a putative LrgB domain related to the control of bacterial XXXX and lysis is required for chloroplast development in @entity958 .

@entity24194 :: ('MESH:D000747', 'Disease') :: ['veinal chlorosis'] @entity958 :: ('3702', 'Species') :: ['Arabidopsis'] @entity3260 :: ('10641', 'Species') :: ['CaMV'] @entity20251 :: ('MESH:D006600', 'Chemical') :: ['hexose phosphates'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity1391 :: ('CHEBI:26020', 'Chemical') :: ['phosphate'] @entity505 :: ('MESH:D002244', 'Chemical') :: ['carbon'] @entity375 :: ('4932', 'Species') :: ['yeast'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death'] @entity8701 :: ('MESH:D009761', 'Chemical') :: ['nystatin']
@entity204 :: (MESH:D003643,Disease) :: ['death']
