Bone morphogenetic proteins (BMPs) and their receptors (BMPRs) are now known to have important roles in @entity1 ovarian folliculogenesis. This study determined the expression of the mRNA encoding for BMPs and their receptors in the @entity1573 ovary and explored possible roles for them. The expression of the mRNA for @entity63150 , and @entity69420 was determined and quantified by a semiquantitative RT-PCR. The mRNAs for all the BMPs and receptors determined were present in both the granulosa (G) and theca (T) cells of the F1, F2, and F3 follicles. All BMP mRNAs increased in G cells with follicular development, whereas only @entity37412 mRNA had this trend in the T cells. @entity69421 mRNAs in T were similar between follicles. BMPR-IA mRNA was similar in F2G and F3G but lower in F1G. BMPR-IB mRNA was similar in G of all follicles, and @entity69420 mRNA increased with development. In the T, each receptor subtype showed equal distribution between follicles. mRNA levels for @entity69420 were higher in G than in T, suggesting that the G is a major target for BMPs. @entity69422 stimulated basal, @entity2090 -, and gonadotropin-stimulated @entity1420 production by cultured G cells, with differential responses between cells from the F1 and F3/4. This suggests involvement in follicular differentiation. @entity69422 reversed the inhibitory effects of transforming growth factor (TGF)-alpha on basal and gonadotropin-stimulated G cell @entity1420 production, with greater effect in the F1 than in the F3/4. This effect suggests an important role for BMPs interacting with @entity20979 in modulating the effects of gonadotropins and @entity2090 on follicular differentiation. Finally, @entity37412 stimulated G cell proliferation, but @entity2089 inhibited @entity20979 + @entity2090 - and/or FSH-stimulated G cell proliferation, suggesting a role in the control of follicular growth during development. These effects of @entity69422 on the G cell function showed relationships with the expression levels of the BMPs and the @entity69420 .

BMPs and BMPRs in @entity1573 ovary and effects of XXXX on granulosa cell proliferation and @entity1420 production in vitro.

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity69421 :: ('378779;396165;420868', 'Gene') :: ['BMP-2, -4, and -6'] @entity63150 :: ('378779;396165', 'Gene') :: ['BMP-2, -4, -6, -7'] @entity1420 :: ('MESH:D011374', 'Chemical') :: ['progesterone'] @entity37412 :: ('395494(Tax:9031)', 'Gene') :: ['BMP-7'] @entity69422 :: ('396165;395494', 'Gene') :: ['BMP-4 and -7'] @entity1573 :: ('9031', 'Species') :: ['chicken'] @entity2089 :: ('396165(Tax:9031)', 'Gene') :: ['BMP-4'] @entity20979 :: ('414743(Tax:9031)', 'Gene') :: ['TGF-alpha'] @entity69420 :: ('374147(Tax:9031)', 'Gene') :: ['BMPR-IB and -II', 'BMPR-IA, -IB, and -II', 'BMPR-II'] @entity2090 :: ('100327329(Tax:9031)', 'Gene') :: ['IGF-I']
@entity69422 :: (396165;395494,Gene) :: ['BMP-4 and -7']
