There is increasing evidence that in the fetal and postnatal development of the adrenal gland, trophic and differentiating effects of ACTH are locally modulated by a species-specific pattern of growth factors. As we have shown previously in @entity1 adult adrenocortical cells (HAC) in culture, @entity442 and, even more, @entity5100 enhance the steroidogenesis and ACTH responsiveness. We now examined the secretion of IGFs and their binding proteins (IGFBP) in the medium of 12 serum free primary cultures of HAC by specific RIAs and [125I]IGF ligand blot or by immunoblot, and their long-term regulation by ACTH. HAC secrete 0.41 and 0.91 ng @entity442 and @entity5100 /5 x 10(5) cells per day, respectively, and their secretion is significantly stimulated 2- and 1.6-fold, respectively, by ACTH. HAC secrete at least three IGFBPs. The 43-46 kDa and the 29 kDa proteins correspond to glycosylated and fragmented forms of @entity2301 , and the 36 kDa protein to @entity10513 . The most abundant protein is the 24 kDa IGFBP, with identical electrophoretic mobility to @entity12561 . @entity2301 , as measured by RIA, is in the range of 1 ng/day. None of the IGFBPs is significantly changed by ACTH. Thus, we have evidence for a local IGF system, and the IGF-levels are in a range compatible with a physiological auto or paracrine action on steroidogenesis.

Is there a local IGF-system in XXXX adrenocortical cells?

@entity1 :: ('9606', 'Species') :: ['human'] @entity12561 :: ('3487', 'Gene') :: ['IGFBP-4'] @entity2301 :: ('3486', 'Gene') :: ['IGFBP-3'] @entity442 :: ('3479', 'Gene') :: ['IGF-I'] @entity10513 :: ('3485', 'Gene') :: ['IGFBP-2'] @entity5100 :: ('3481', 'Gene') :: ['IGF-II']
@entity1 :: (9606,Species) :: ['human']
