OBJECTIVE: To explore new methods for the early diagnosis of @entity788 through detection of @entity984 and @entity876 mutations in pancreatic juice and stool. METHODS: 201 @entity1 in PUMC Hospital from 1994 - 2000 and 60 control individuals were enrolled in this study. @entity984 point mutation was detected by PCR-RFLP while @entity876 mutation was detected by PCR-SSCP. RESULTS: @entity984 mutation was found in pancreatic juice in 87.8% (36/41) of @entity788 @entity1 and 23.5% (4/17) of benign @entity660 @entity1 . In 261 stool specimens, amplification found mutations successfully in 235 @entity1 (90%). @entity984 mutation was found in stool in 88% (66/75) of @entity788 @entity1 , 51.1% (24/47) of benign @entity660 @entity1 and 19.6% (9/46) of normal individuals. @entity876 mutation was found in pancreatic juice in 47.4% (18/38) of @entity788 @entity1 and 12.5% (2/16) of benign @entity660 @entity1 . @entity876 mutation was found in stool in 37.1% (23/62) and 19.1% (4/21) of chronic @entity788 @entity1 . CONCLUSION: @entity984 mutation in pancreatic juice has higher diagnosis sensitivity and specificity, and therefore may be used as a supplement in the diagnosis of @entity788 . Detection of @entity984 mutation combined with @entity876 mutation in stool can aid in the screening of @entity788 .

Detecting XXXX and @entity876 gene mutation from stool and pancreatic juice for diagnosis of early @entity788 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity788 :: ('MESH:D010190', 'Disease') :: ['pancreatitis', 'pancreatic cancer'] @entity984 :: ('3845', 'Gene') :: ['K-ras'] @entity660 :: ('MESH:D010182', 'Disease') :: ['pancreatic disease'] @entity876 :: ('7157', 'Gene') :: ['p53']
@entity984 :: (3845,Gene) :: ['K-ras']
