AIMS: The pathogenic potential of @entity8177 (A.) butzleri isolates on @entity1 (HT-29/B6) and porcine epithelial (IPEC- @entity1 ) cells was investigated by in vitro assays. METHODS AND RESULTS: Five out of six A.  butzleri isolates were able to adhere and invade HT-29/B6 cells while only four isolates adhered and two invaded IPEC- @entity1 cells. Two non- or poorly invasive A.  butzleri isolates were highly cytotoxic to differentiated HT-29/B6 cells but none to IPEC- @entity1 cells as determined by WST-assays. Epithelial integrity of cell monolayers, monitored by measurement of the transepithelial electrical resistance (TER), was decreased by all A.  butzleri isolates in HT-29/B6 and IPEC- @entity1 cells to 30 -15  % and 90  - 50%, respectively. CONCLUSION: The A.  butzleri strain-specific pathomechanisms observed with the @entity1 colon cell line HT-29/B6, like adhesion, invasion and @entity137 might all contribute to epithelial barrier dysfunction, which could explain a leak-flux type of @entity294 in @entity1 . In contrast, porcine cells seem to be less susceptible to A.  butzleri. SIGNIFICANCE AND IMPACT OF STUDY: A.  butzleri has enteric pathogenic potential, characterized by defined interactions with @entity1 epithelial cells and strain-specific pathomechanisms. This article is protected by copyright. All rights reserved.

XXXX isolates exhibit pathogenic potential in intestinal epithelial cell models.

@entity8177 :: ('28197', 'Species') :: ['Arcobacter'] @entity1 :: ('9606', 'Species') :: ['humans', 'human', 'J2'] @entity294 :: ('MESH:D003967', 'Disease') :: ['diarrhea'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity']
@entity8177 :: (28197,Species) :: ['Arcobacter butzleri']
