Both @entity172 and leptin play an important role in the regulation of physiological processes of endochondral bone formation in linear growth. @entity172 receptors ( @entity1782 and @entity1783 ) are known as members of the superfamily of nuclear @entity134 hormone receptors and are detected in all zones of growth plate chondrocytes. They can be regulated in a ligand-independent manner. Whether leptin regulates ERs in the growth plate is still not clear. To explore this issue, chondrogenic ATDC5 cells were used in the present study. Messenger RNA and protein analyses were performed by quantitative PCR and Western blotting. We found that both @entity1782 and @entity1783 were dynamically expressed during the ATDC5 cell differentiation for 21 days. Leptin (50 ng/ml) significantly upregulated @entity1782 and @entity1783 mRNA and protein levels 48 h after leptin stimulation (P<0.05) at day 14. The up-regulation of @entity1782 and @entity1783 mRNA by leptin was shown in a dose-dependent manner, but the most effective dose of leptin was different (100 and 1,000 ng/ml, respectively). Furthermore, we confirmed that leptin augmented the phosphorylation of @entity1366 in a time-dependent manner. A maximum eightfold change was observed at 15 min. Finally, a specific @entity1366 inhibitor, @entity5732 , blocked leptin-induced ERs regulation in ATDC5 cells, indicating that @entity1366 mediates, partly, the effects of leptin on ERs. These data demonstrate, for the first time, that leptin regulates the expression of ERs in growth plate chondrocytes via ERK signaling pathway, thereby suggesting a crosstalk between leptin and @entity172 receptors in the regulation of bone formation.

Leptin regulates XXXX receptor gene expression in ATDC5 cells through the extracellular signal regulated kinase signaling pathway.

@entity172 :: ('MESH:D004967', 'Chemical') :: ['estrogen', 'Estrogen'] @entity1782 :: ('2099', 'Gene') :: ['ERa'] @entity5732 :: ('MESH:C113580', 'Chemical') :: ['UO126'] @entity1783 :: ('2100', 'Gene') :: ['ERb'] @entity134 :: ('CHEBI:35341', 'Chemical') :: ['steroid'] @entity1366 :: ('5595;5594', 'Gene') :: ['ERK1/2']
@entity172 :: (MESH:D004967,Chemical) :: ['estrogen']
