UNASSIGNED: Multidrug-resistant (MDR) @entity3064 is an opportunistic @entity1 pathogen that has become highly problematic in the clinical environment. Novel therapies are desperately required. To assist in identifying new therapeutic targets, the antagonistic interactions between A. baumannii and the most common @entity1 fungal pathogen, @entity5132 , were studied. We have identified that the @entity5132 quorum-sensing molecule, @entity37389 , has cross-kingdom interactions, affecting the viability of A. baumannii. To gain an understanding of its mechanism, the transcriptional profile of A. baumannii exposed to @entity37389 was examined. @entity37389 caused dysregulation of a large number of genes involved in cell membrane biogenesis, multidrug efflux pumps (AcrAB-like, AdeIJK-like), and A. baumannii virulence traits such as biofilm formation (csuA, csuB, ompA) and motility (pilZ, pilH). We also observed a strong induction in genes involved in cell division (minD, minE, ftsK, ftsB, and ftsL). These transcriptional data were supported by functional assays, showing that @entity37389 disrupts A. baumannii cell membrane integrity, alters cell morphology, and impairs virulence characteristics such as biofilm formation and twitching motility. Moreover, we show that A. baumannii uses efflux pumps as a defence mechanism against this eukaryotic signalling molecule. Owing to its effects on membrane integrity, @entity37389 was tested to see if it potentiated the activity of the membrane-acting polymyxin antibiotic, @entity3273 . When co-administered, @entity37389 increased sensitivity to @entity3273 for otherwise resistant strains. These data provide mechanistic understanding into the antagonistic interactions between diverse pathogens and may provide important insights into novel therapeutic strategies.

The Impact of a Cross-Kingdom Signalling Molecule of XXXX on @entity3064 physiology.

@entity1 :: ('9606', 'Species') :: ['human'] @entity3273 :: ('MESH:D003091', 'Chemical') :: ['colistin'] @entity5132 :: ('5476', 'Species') :: ['Candida albicans', 'C. albicans'] @entity37389 :: ('MESH:D005204', 'Chemical') :: ['Farnesol', 'farnesol'] @entity3064 :: ('470', 'Species') :: ['Acinetobacter baumannii']
@entity5132 :: (5476,Species) :: ['Candida albicans']
