UNASSIGNED: Vascular smooth muscle (VSM) expresses @entity460 /calmodulin-dependent protein kinase II (CaMKII)-   and -y isoforms. CaMKII   promotes VSM proliferation and vascular remodeling. We tested CaMKIIy function in vascular remodeling after injury. CaMKIIy protein decreased 90% 14 d after @entity130 in @entity35 carotid artery. Intraluminal transduction of adenovirus encoding CaMKIIyC rescued expression to 35% of uninjured controls, inhibited @entity8233 formation (>70%), inhibited VSM proliferation (>60%), and increased expression of the cell-cycle inhibitor @entity7504 (>2-fold). Comparable doses of CaMKII  2 adenovirus had no effect. Similar dynamics in CaMKIIy mRNA and protein expression were observed in ligated @entity19 carotid arteries, correlating closely with expression of VSM differentiation markers. Targeted deletion of CaMKIIy in smooth muscle resulted in a 20-fold increase in neointimal area, with a 3-fold increase in the cell proliferation index, no change in apoptosis, and a 60% decrease in @entity7504 expression. In cultured VSM, CaMKIIy overexpression induced @entity10740 mRNA (1.7 fold) and protein (1.8-fold) expression; induced the @entity10740 target gene @entity7504 (3-fold); decreased VSM cell proliferation (>50%); and had no effect on expression of apoptosis markers. We conclude that regulated CaMKII isoform composition is an important determinant of the injury-induced vasculoproliferative response and that CaMKIIy and -   isoforms have nonequivalent, opposing functions.-Saddouk, F. Z., Sun, L.-Y., Liu, Y. F., Jiang, M., Singer, D. V., Backs, J., Riper, D. V., Ginnan R., Schwarz, J. J., Singer, H. A. @entity141 /calmodulin-dependent protein kinase II-y (CaMKIIy) negatively regulates vascular smooth muscle cell proliferation and vascular remodeling.

XXXX /calmodulin-dependent protein kinase II-y (CaMKIIy) negatively regulates vascular smooth muscle cell proliferation and vascular remodeling.

@entity10740 :: ('301300(Tax:10116)', 'Gene') :: ['p53'] @entity7504 :: ('24525(Tax:10116)', 'Gene') :: ['p21'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity130 :: ('MESH:D014947', 'Disease') :: ['balloon injury'] @entity8233 :: ('MESH:D058426', 'Disease') :: ['neointima']
@entity141 :: (CHEBI:29108,Chemical) :: ['Ca2+']
