Multiple molecular forms of @entity3776 dehydrogenase (G6PDH) were detected by activity staining in non-denaturing @entity2975 gels of cell-free extracts from a range of cyanobacteria including @entity13931 , @entity14221 , @entity26746 PCC 73110, @entity324 , Nostoc sp. MAC PCC 8009 and the marine strain @entity54244 . In most of the species tested, the profile of G6PDH activities was modulated by the growth of the cells in the presence of exogenous 10 mM @entity413 . Using an antiserum raised against a fragment of G6PDH from @entity13931 , it was shown that the different molecular forms of G6PDH all contained an antigenically related subunit, suggesting that the different forms arose from different quaternary structures involving the same monomer. An insertion mutant of @entity14221 was constructed in which the opcA gene, adjacent to zwf (encoding G6PDH), was disrupted. Although no reduction in the amount of G6PDH monomers (Zwf) was observed in the opcA mutant, activity staining of native gels indicated that most of this protein is not assembled into one of the active oligomeric forms. The oligomerization of G6PDH in extracts of the opcA mutant was stimulated in vitro by a factor present in crude extracts of the wild-type, suggesting that the product of the opcA gene is involved in the oligomerization and activation of G6PDH.

Multiple oligomeric forms of XXXX dehydrogenase in cyanobacteria and the role of OpcA in the assembly process.

@entity26746 :: ('1184', 'Species') :: ['Plectonema boryanum'] @entity54244 :: ('32051', 'Species') :: ['Synechococcus sp. WH7803'] @entity13931 :: ('103690', 'Species') :: ['Anabaena sp. PCC 7120'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity324 :: ('1148', 'Species') :: ['Synechocystis sp. PCC 6803'] @entity14221 :: ('1140', 'Species') :: ['Synechococcus sp. PCC 7942'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity3776 :: ('MESH:D019298', 'Chemical') :: ['glucose-6-phosphate']
@entity3776 :: (MESH:D019298,Chemical) :: ['glucose-6-phosphate']
