Both flagellin (fliC) and @entity6026 (INF-y-inducing factor) have been developed as adjuvants for improving immunogenicity in DNA-vaccinated hosts. An @entity2858 gag plasmid encodes a protein harboring broad epitopes for cytotoxic T-lymphocytes. In this study, the immunogenicity of BALB/c @entity19 immunized with an @entity2858 gag plasmid (pVAX/gag) combined with a chimeric plasmid encoding @entity6026 fused to flagellin (pcDNA3/ @entity6026 _fliC) or a single plasmid encoding @entity6026 (pcDNA3/ @entity6026 ) and/or flagellin (pcDNA3/fliC) was assessed. Through in vitro transcription and translation, it was demonstrated that both mRNA and protein were appropriately expressed by each construct. The @entity6026 and flagellin fusion protein, which could be detected in supernatants from transfected cells, was effective in inducing IFN-y by lymphocytes. Following i.m. immunization, expressions of flagellin or @entity6026 were detected in muscle cells by immunohistochemistry analysis from 72   hr. At 12 weeks post-immunization, both gag-specific IgG in sera and spleen cell proliferation were high in all @entity19 groups. However, the @entity9749 / @entity6527 ratio, @entity630 cytokine ( @entity2047 ) production and proportion of gag-specific CD3(+) CD8(+) IFN-y-secreting cells were significantly higher in the @entity19 group co-immunized with pVAX/gag plasmid and pcDNA3/ @entity6026 _fliC than in the @entity19 immunized with pVAX/gag plasmid combined with either pcDNA3/fliC or @entity6026 plasmid or both. These findings suggest that a chimeric plasmid encoding @entity6026 fused to flagellin can be used as an adjuvant-like plasmid to improve the @entity630 immune response, particularly for induction of CD3(+) CD8(+) IFN-y-secreting cells in gag plasmid-vaccinated @entity19 .

Improvement in T helper 1-related immune responses in BALB/c @entity19 immunized with an XXXX gag plasmid combined with a chimeric plasmid encoding interleukin-18 and flagellin.

@entity6026 :: ('16173(Tax:10090)', 'Gene') :: ['IL-18', 'pcDNA3/IL-18'] @entity2047 :: ('16183(Tax:10090)', 'Gene') :: ['IL-2 and IFN-y'] @entity2858 :: ('11676', 'Species') :: ['HIV-1'] @entity19 :: ('10090', 'Species') :: ['mice', 'murine'] @entity6527 :: ('105243590', 'Gene') :: ['IgG1'] @entity630 :: ('57314(Tax:10090)', 'Gene') :: ['Th1'] @entity9749 :: ('668478(Tax:10090)', 'Gene') :: ['IgG2a']
@entity2858 :: (11676,Species) :: ['HIV-1']
