OBJECTIVE: @entity1797 ( @entity1797 ) can lead to symptomatic conditions including @entity615 . The purpose of this study is to understand the extracellular matrix (ECM) changes in disc biology through comparative proteomic analysis of degenerated and non-degenerated @entity1 intervertebral disc (IVD) tissues of different ages. DESIGN: Seven non-degenerated (11-46 years of age) and seven degenerated (16-53 years of age) annulus fibrosus (AF) and nucleus pulposus (NP) samples were used. Proteins were extracted using @entity2097 , separated from large proteoglycans (PGs) by @entity11680 ( @entity6928 ) density gradient ultracentrifugation, and identified using liquid chromatography (LC) coupled with tandem mass spectrometry ( @entity15 / @entity15 ). For quantitative comparison, proteins were labeled with iTRAQ reagents. Collagen fibrils in the NP were assessed using @entity4653 ( @entity4653 ). RESULTS: In the AF, quantitative analysis revealed increased levels of @entity13588 , COMP and @entity56030 in degeneration when compared with samples from older individuals. Fibronectin showed increment with age and degeneration. In the NP, more @entity56030 and @entity66660 were present in degenerated samples of younger individuals. Reduced protein solubility was observed in degenerated and older non-degenerated samples correlated with an accumulation of type I collagen in the insoluble fibers. Characterization of collagen fibrils in the NP revealed smaller mean fibril diameters and decreased porosity in the degenerated samples. CONCLUSIONS: Our study identified distinct matrix changes associated with aging and degeneration in the @entity1798 ( @entity1798 ). The nature of the ECM changes, together with observed decreased in solubility and changes in fibril diameter is consistent with a fibrotic-like environment.

Fibrotic-like changes in degenerate XXXX @entity1798 revealed by quantitative proteomic analysis.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1797 :: ('MESH:D055959', 'Disease') :: ['Intervertebral disc degeneration', 'IDD'] @entity11680 :: ('CHEBI:63039', 'Chemical') :: ['caesium chloride'] @entity56030 :: ('8483', 'Gene') :: ['CILP'] @entity2097 :: ('MESH:D019791', 'Chemical') :: ['guanidine hydrochloride'] @entity615 :: ('MESH:D001416', 'Disease') :: ['sciatica and back pain'] @entity6928 :: ('MESH:C028019', 'Chemical') :: ['CsCl'] @entity66660 :: ('148113', 'Gene') :: ['CILP2'] @entity1798 :: ('MESH:C535531', 'Disease') :: ['intervertebral discs', 'IVDs'] @entity15 :: ('MESH:D009103', 'Disease') :: ['MS'] @entity13588 :: ('5654', 'Gene') :: ['HTRA1'] @entity4653 :: ('MESH:D004401', 'Disease') :: ['SEM', 'scanning electron microscopy']
@entity1 :: (9606,Species) :: ['human']
