@entity19 @entity20736 is associated with increased adherence of neutrophils to the vascular endothelium resulting in neutrophil infiltration and tissue damage. We examined the effects of @entity130 on the expression of the adhesion molecule P-selectin in several vital organs (i.e., heart, lungs, liver, kidneys, and small intestine) 2 h after induction of Noble-Collip drum @entity130 in anesthetized @entity35 . Total RNA was extracted from these organs and P-selectin mRNA was quantified by RNase protection assays. P-selectin mRNA was significantly increased over control nontraumatized, anesthetized @entity35 in all vital organs (P<0.05 or less), with the largest increase occurring in the lung (P<0.01). Immunohistochemical analysis showed increased expression of P-selectin protein in postcapillary venules of all vital organs after @entity130 . To further investigate the possible mechanisms of increased P-selectin mRNA transcription promoter activity during @entity130 , we quantified binding of proteins from nuclear extracts to the kappaB site (-218GGGGGTGACCC[-207]) of the P-selectin gene by electrophoretic mobility shift assay. We confirmed the results of NF-kappaB binding by demonstrating increases in p50 and @entity12992 , as well as decreases in IkappaB in cytoplasmic and nuclear extracts from the lungs of @entity130 @entity35 by Western blotting. Increased activity of the transcription factor, nuclear factor kappaB (NF-kappaB), occurred in all vital organs of the @entity130 @entity35 compared to sham-operated controls. Our findings suggest that severe @entity130 results in up-regulation of P-selectin at the transcriptional level, which is partly controlled by an NF-kappaB-responsive element in the region of the P-selectin promoter. This increased activation of NF-kappB binding may contribute to the widespread increases in P-selectin expression observed in several vital organs 2 h after @entity130 , which in turn may play a key role in the pathogenesis of @entity20736 .

P-selectin is up-regulated in vital organs during @entity19 XXXX .

@entity19 :: ('10090', 'Species') :: ['Murine'] @entity12992 :: ('317414(Tax:10116)', 'Gene') :: ['p52'] @entity20736 :: ('MESH:D012774', 'Disease') :: ['traumatic shock'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity130 :: ('MESH:D014947', 'Disease') :: ['trauma']
@entity20736 :: (MESH:D012774,Disease) :: ['traumatic shock']
