OBJECTIVES: Beta-cell @entity3210 ( @entity1689 ) is considered as a late manifestation emanating from @entity82 . To ascertain the role of T-helper cells in b-cell dysfunction, we enumerated circulating T-cell subsets, examined their infiltration into pancreatic islets, and assessed islet functions. METHODS: Pancreatic tissues and peripheral blood were obtained from @entity1689 @entity1 with/without @entity6 . T cells were enumerated on flow cytometry and by immunostaining. Islets were assessed for @entity413 -stimulated @entity2690 release (GSIR) and apoptosis ( @entity4404 / @entity522 ). Islet proteins were probed for @entity2690 gene transcription factor. RESULTS: Circulating @entity1198 ) cells were higher (P < 0.003) in @entity1689 @entity1 with @entity6 in comparison with control and @entity1689 @entity1 without @entity6 . Intra-islet colocalization of Th1 and Th17 cells was evident. In comparison with the controls, 2%    0.87% b cells from @entity1689 @entity1 without @entity6 were apoptotic whereas GSIR was decreased by 60%    12%, and 40%    9% from @entity1689 @entity1 with @entity6 were apoptotic, with minimal GSIR (1.42%    0.9%) in the remaining 60% viable cells. Western blots of islet proteins revealed an increase in @entity6897 ( @entity6897 ) and a decrease in phosphorylated pancreatic duodenal homeobox ( @entity5908 ). CONCLUSIONS: T cell-mediated @entity32 is associated with b-cell dysfunction during progression of @entity1689 .

XXXX Contributes to b-Cell Dysfunction in Chronic Pancreatitis.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis'] @entity522 :: ('836', 'Gene') :: ['caspase-3'] @entity5908 :: ('3651', 'Gene') :: ['Pdx-1'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetes'] @entity4404 :: ('308', 'Gene') :: ['Annexin V'] @entity1689 :: ('MESH:D050500', 'Disease') :: ['CP'] @entity2690 :: ('3630', 'Gene') :: ['insulin'] @entity1198 :: ('51497', 'Gene') :: ['T-helper type 1 (Th1'] @entity3210 :: ('MESH:D010188', 'Disease') :: ['dysfunction and endocrine insufficiency in chronic pancreatitis'] @entity6897 :: ('6772', 'Gene') :: ['signal transducer and activator of transcription 1', 'STAT1'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity32 :: (MESH:D007249,Disease) :: ['T-Helper Cell-Mediated Islet Inflammation']
