The standard method of detecting neurogenesis uses @entity1603 ( @entity1603 ) to label DNA synthesis followed by double labeling with neuronal markers. However, DNA synthesis may occur in events unrelated to neurogenesis including aneuploidy and abortive cell cycle reentry. Hence, it is important to confirm neurogenesis with methods other than @entity1603 incorporation. To this end, we have generated transgenic nestin-CreER @entity19 that express @entity440 -inducible Cre recombinase under the control of a nestin enhancer. When crossed with a ubiquitous Enhanced Green Fluorescent Protein (EGFP)-Cre-reporter line, the bitransgenic animals can reveal the nestin-positive progenitors and their progeny with EGFP after @entity440 induction. This system has many applications including visualization of embryonic neural progenitors, detection of postnatally transformed radial glial cells, and labeling adult neural progenitors in the subventricular zone (SVZ). To examine the contribution of SVZ progenitors to cell replacement after @entity64 , @entity440 -induced @entity19 were challenged with focal @entity63 or combined @entity63 - @entity519 followed by @entity1603 injection. This analysis revealed only very few EGFP-positive cells outside the SVZ after focal @entity63 but robust DNA synthesis by hippocampal neurons without immediate cell @entity204 following @entity63 - @entity519 . These results suggest that the nestin-CreER system is a useful tool for detecting embryonic and adult neurogensis. They also confirm the existence of nonproliferative DNA synthesis by old neurons after experimental @entity1545 .

Nestin-CreER XXXX reveal DNA synthesis by nonapoptotic neurons following cerebral ischemia hypoxia.

@entity1545 :: ('MESH:D020214', 'Disease') :: ['brain injury'] @entity1603 :: ('MESH:D001973', 'Chemical') :: ['BrdU', 'bromodeoxyuridine'] @entity64 :: ('MESH:D020521', 'Disease') :: ['stroke'] @entity440 :: ('MESH:D013629', 'Chemical') :: ['tamoxifen'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemia'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity519 :: ('MESH:D000860', 'Disease') :: ['hypoxia'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death']
@entity19 :: (10090,Species) :: ['mice']
