Candida boidinii produces significant amounts of @entity8372 from @entity6122 , and assays of crude homogenates for @entity3650 ( @entity6122 ) reductase (XYL1p) have been reported to show relatively high activity with @entity2125 as a cofactor even though XYL1p purified from this @entity375 does not have such activity. A gene coding for XYL1p from C. boidinii (CbXYL1) was isolated by amplifying the central region using primers to conserved domains and by genome walking. CbXYL1 has an open reading frame of 966 bp encoding 321 @entity1522 . The C. boidinii XYL1p is highly similar to other known @entity375 @entity3650 reductases and is most closely related to the @entity1110 -linked XYL1p of @entity11900 . Cell homogenates from C. boidinii and recombinant @entity375 were tested for XYL1p activity to confirm the previously reported high ratio of @entity2125 : @entity1110 linked activity. C. boidinii grown under fully aerobic conditions showed an @entity2125 : @entity1110 activity ratio of 0.76, which was similar to that observed with the XYL1p from @entity28558 XYL1, but which is much lower than what was previously reported. Cells grown under low aeration showed an @entity2125 : @entity1110 activity ratio of 2.13. Recombinant @entity375 expressing CbXYL1 showed only @entity2125 -linked activity in cell homogenates. Southern hybridization did not reveal additional bands. These results imply that a second, unrelated gene for XYL1p is present in C. boidinii.

Molecular characterization of a gene for @entity3650 reductase ( CbXYL1) from Candida boidinii and its expression in XXXX .

@entity6122 :: ('MESH:D014994', 'Chemical') :: ['xylose'] @entity2125 :: ('MESH:D009243', 'Chemical') :: ['NADH'] @entity8372 :: ('MESH:D014993', 'Chemical') :: ['xylitol'] @entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity1110 :: ('MESH:D009249', 'Chemical') :: ['NAD(P)H', 'NADPH'] @entity28558 :: ('4924', 'Species') :: ['Pichia stipitis'] @entity3650 :: ('CHEBI:15693', 'Chemical') :: ['aldose'] @entity11900 :: ('28985', 'Species') :: ['Kluyveromyces lactis'] @entity375 :: ('4932', 'Species') :: ['yeast', 'Saccharomyces cerevisiae', 'S. cerevisiae']
@entity375 :: (4932,Species) :: ['Saccharomyces cerevisiae']
