Intraepithelial lymphocytes (IELs) are considered to drive immune surveillance of the epithelial layer to the mucosa, which is initially exposed to exogenous antigens. However, how IELs are activated by orally administered antigens remains unclear. To clarify this mechanism, we fed ovalbumin (OVA) to T cell receptor transgenic (TCR-Tg) @entity19 with OVA-specific MHC class II-restricted TCR and found that the cytotoxic activity of IELs was increased against both @entity30414 target cells, but notably reduced after depleting CD8 + IELs. Cytoplasmic staining showed that the production of @entity60 and @entity2047 was increased in @entity19 fed with OVA both in the supernatant of cultured IELs with immobilized anti- @entity31887 mAb and in fresh @entity1961 + IELs. In contrast, the cytotoxic activity against @entity30414 target cells and the production of @entity2047 and @entity60 was decreased in @entity1619 cells from @entity19 fed with OVA. However, when the @entity1619 cells from these @entity19 were cultured with OVA and @entity2047 , @entity60 production recovered. The decreased response demonstrated the clonal anergy of T cells. Furthermore, @entity5 growth was enhanced in TCR-Tg @entity19 carrying an OVA-transfected counterpart @entity47959 @entity1892 (OVA- @entity47959 ) and fed with OVA. These results indicate that the oral administration of soluble antigens can activate @entity1961 + IELs in an antigen-specific manner but induces @entity6306 in the spleen. In addition, @entity630 -type cytokines produced by activated @entity1961 + IEL might provide a bystander effect on the cytotoxic activity of IELs.

Oral antigen induces antigen-specific activation of intraepithelial XXXX + lymphocytes but suppresses their activation in spleen.

@entity31887 :: ('12503(Tax:10090)', 'Gene') :: ['CD3'] @entity6306 :: ('MESH:C566417', 'Disease') :: ['hyporesponsiveness'] @entity1619 :: ('MESH:D015458', 'Disease') :: ['splenic T'] @entity2047 :: ('16183(Tax:10090)', 'Gene') :: ['IL-2'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity60 :: ('15978(Tax:10090)', 'Gene') :: ['IFN-gamma'] @entity1961 :: ('12504(Tax:10090)', 'Gene') :: ['CD4'] @entity1892 :: ('MESH:D016393', 'Disease') :: ['B cell lymphoma'] @entity47959 :: ('21929(Tax:10090)', 'Gene') :: ['A20'] @entity30414 :: ('71481(Tax:10090)', 'Gene') :: ['NK and LAK'] @entity630 :: ('57314(Tax:10090)', 'Gene') :: ['Th1']
@entity1961 :: (12504(Tax:10090),Gene) :: ['CD4']
