UNASSIGNED: @entity40807 (NF) is one of the main aporphine alkaloids existing in the traditional Chinese medicine Folium Nelumbinis (lotus leaves). Modern pharmacological studies have demonstrated that NF has a broad spectrum of bioactivities, such as anti- @entity778 and anti-hyperlipidemic effects, and has been recommended as a leading compound for new drug development. However, the metabolites and biotransformation pathway of NF in vivo have not yet been comprehensively investigated. The present study was performed to identify the metabolites of NF for exploring in vivo fates. @entity35 plasma and urine samples were collected after oral administration and prepared by liquid-liquid extraction with @entity1046 . A method based on ultra fast liquid chromatography with tandem mass spectrometry was applied to identify the metabolites. Q1 (first quadrupole) full scan combined with multiple reaction monitoring (MRM) survey scan were used for the detection of metabolites. MRM-information dependent acquisition (IDA) of enhanced product ions (MRM-IDA-EPI) was used for the structural identification of detected metabolites. A total of ten metabolites were identified, including phase I (demethylation, oxidation and dehydrogenation) and phase II (glucuronidation, sulfation and @entity2041 ) biotransformation products. In which, demethylation is the main metabolic pathway of NF in the body. The results can help to better understand the disposition and pharmacological mechanism of NF in the body. This article is protected by copyright. All rights reserved.

Metabolism profiles of XXXX in @entity35 by using ultra fast liquid chromatography with tandem mass spectrometry.

@entity778 :: ('MESH:D015658', 'Disease') :: ['HIV'] @entity1046 :: ('MESH:C007650', 'Chemical') :: ['ethyl acetate'] @entity40807 :: ('MESH:C008692', 'Chemical') :: ['Nuciferine'] @entity2041 :: ('MESH:D005978', 'Chemical') :: ['glutathione'] @entity35 :: ('10116', 'Species') :: ['Rat']
@entity40807 :: (MESH:C008692,Chemical) :: ['nuciferine']
