Gene transfer into the peritoneal cavity by nonviral methods may provide an effective therapeutic approach for @entity6378 . Herein, we investigated the feasibility and the effectiveness of ultrasound-microbubble-mediated delivery of naked plasmid DNA into the peritoneal cavity in @entity35 . Following the intraperitoneal or the intravenous administration of a mixture of plasmid DNA (100 microg) and ultrasound contrast agent microbubbles, an ultrasound transducer was applied on the abdominal wall. The reporter pTRE plasmid encoding @entity39685 was used to evaluate transfection efficiency. @entity39685 expression was induced by @entity3403 in drinking water. We detected less than 10% apoptotic cells and no inflammatory reaction in peritoneal tissues following the ultrasound-microbubble-mediated transfection. More importantly, the insonation significantly improved the transfection efficiency in peritoneal tissues. The transfection efficiency by intraperitoneal delivery route was higher than the intravenous route. The reporter gene, pTRE- @entity39685 , was readily detected in the parietal peritoneum, mesentery, greater omentum and adipose tissue. The peak of transgene expression occurred 2 days after transfection and the transgene expression diminished in a time-dependent manner thereafter. Overall, the effectiveness and simplicity of the ultrasound-microbubble-mediated system may provide a promising nonviral means for improving gene delivery for treating @entity6378 in vivo.

Ultrasound-contrast agent mediated naked gene delivery in the peritoneal cavity of adult XXXX .

@entity35 :: ('10116', 'Species') :: ['rats'] @entity3403 :: ('MESH:D004318', 'Chemical') :: ['doxycycline'] @entity6378 :: ('MESH:D010532', 'Disease') :: ['peritoneal diseases'] @entity39685 :: ('81516(Tax:10116)', 'Gene') :: ['Smad7']
@entity35 :: (10116,Species) :: ['rat']
