OBJECTIVE: The diagnosis and follow-up of @entity1 with T1 @entity150 relies invasive procedures. We developed a non-invasive method for detection of T1 @entity150 based on a feasible non-radioactive molecular approach. MATERIALS AND METHODS: Urine DNA samples were collected from 24 @entity1 with @entity1606 ( @entity1606 ) of the bladder and were processed by denaturing gradient gel electrophoresis (DGGE) analysis. Urine samples obtained from 10 individuals with no clinical evidence of @entity5 were used as controls. RESULTS: Ten @entity1 out of 24 (41%) had @entity876 mutations in their @entity5 samples. Seven of these presented the same mutation in matched urine samples. The @entity876 mutation pattern found in urine was always identical to that identified in the @entity5 . Decision diagnostic criteria showed that molecular screening by DGGE of amplified DNA from urine sediment had 69.2 % sensitivity, 100% specificity, 95.8 % accuracy, 100% prediction of positive result and 95.4 % prediction of negative result, respectively. No @entity876 mutation was found in the urine from control subjects. CONCLUSION: DGGE analysis of urine samples could be a useful tool for the early detection of T1 @entity150 or its recurrence, potentially leading to a reduction in the frequency of invasive procedures used for the management of this disease.

Diagnosis of XXXX by denaturing gradient gel electrophoresis urinalysis.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'genitourinary malignancy', 'primary tumor'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity150 :: ('MESH:D001749', 'Disease') :: ['bladder cancer'] @entity1606 :: ('MESH:D002295', 'Disease') :: ['TCC', 'T1 transitional cell carcinoma']
@entity150 :: (MESH:D001749,Disease) :: ['T1 bladder transitional cell carcinoma']
