OBJECTIVE: This study aims to measure the expression of toll-like receptors (TLR) in monocyte-derived dendritic cells (MoDC) from chronic severe @entity1956 (CSHB), to assess the contribution of TLRs in CSHB. METHODS: Peripheral blood was collected from 40 CSHB @entity1 , 30 @entity6194 ( @entity6194 ) @entity1 , and 30 healthy individuals who served as healthy controls (HCs). Purified monocytes were isolated by a combination of Histopaque-1.077 and @entity5750 Microbeads. MoDCs were induced with @entity2841 and @entity3646 for 6 days from @entity5750 (+) monocytes. The expression of TLRs in MoDC was measured using real-time PCR and flow cytometry. RESULTS: The expressions of @entity37047 , -7 were significantly higher in MoDC of CSHB than that of HCs, of which the level of @entity6800 was decreased. Particularly in CSHB @entity1 , the @entity6800 expression was further decreased compared to @entity6194 @entity1 . In non-survival CSHB @entity1 , @entity6800 level was significantly decreased, while @entity2938 expression was dramatically increased. Linear correlation analysis demonstrated significant correlations between @entity6800 level and disease severity markers (total @entity648 , prothrombin activity, @entity1011 , white blood cell count, and maximum volume of ascitic fluid) in individual CSHB @entity1 . CONCLUSIONS: @entity2938 and @entity6800 may be involved in the pathogenesis of CSHB, and @entity6800 may influence the prognosis of CSHB.

Correlation of the expression of toll-like receptors in monocyte-derived dendritic cells with prognosis of chronic severe XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity6800 :: ('7098', 'Gene') :: ['TLR-3'] @entity1956 :: ('MESH:D006509', 'Disease') :: ['hepatitis B'] @entity5750 :: ('929', 'Gene') :: ['CD14'] @entity648 :: ('MESH:D001663', 'Chemical') :: ['bilirubin'] @entity6194 :: ('MESH:D019694', 'Disease') :: ['chronic hepatitis B', 'CHB'] @entity37047 :: ('7096;7097', 'Gene') :: ['TLR-1, -2'] @entity2938 :: ('7097', 'Gene') :: ['TLR-2'] @entity1011 :: ('MESH:D003404', 'Chemical') :: ['creatinine'] @entity3646 :: ('3565', 'Gene') :: ['interleukin-4'] @entity2841 :: ('1437', 'Gene') :: ['granulocyte macrophage colony-stimulating factor']
@entity1956 :: (MESH:D006509,Disease) :: ['hepatitis B']
