We have previously reported that @entity12386 , an actin-binding protein, plays an important role in phagocyte functions, such as respiratory burst, phagocytosis, and @entity6722 . On the other hand, it was recently found that @entity21357 ( @entity21357 ) phosphorylates @entity12386 . In this work, we investigated the roles of @entity21357 in activated phagocytes. The results of immunostaining showed that in dormant phagocytes the endogenous @entity21357 was diffusely distributed in the cytosol of macrophage-like U937 cells, and when activated by opsonized zymosan (OZ), it was translocated to plasma membranes. Green fluorescence protein (GFP)-conjugated @entity21357 was expressed in the phagocytes, and the GFP-positive cells were isolated by a fluorescence-activated cell sorter. The isolated wild-type @entity21357 -overexpressing cells produced @entity224 at a rate that was 3.2-fold higher than that of only GFP-expressing control cells, whereas the respiratory burst of dominant negative @entity21357 ( @entity69394 )-expressing cells decreased to 31% of that of the control cells. Phagocytic activity monitored by using @entity18523 -labeled OZ was also decreased in the @entity69394 -expressing cells. By immunoblotting using a specific anti-phosphorylated @entity12386 antibody, it was revealed that in the OZ-activated wild-type @entity21357 -GFP-expressing cells, the phosphorylated @entity12386 increased by 2.3-fold, and that in the OZ-activated @entity69394 -GFP-expressing cells, the phosphorylated @entity12386 decreased to 47% of that of only GFP-expressing cells (mock control). Furthermore, in the wild-type @entity21357 -expressing cells, OZ-evoked increase in filamentous actin was markedly enhanced, whereas in the dominant negative @entity21357 -expressing cells, the total level of F-actin was strongly suppressed. These results suggest that @entity21357 regulates the functions of phagocytes through phosphorylation of @entity12386 and enhances the formation of filamentous actin.

LIM kinase 1 modulates opsonized zymosan-triggered activation of macrophage-like U937 cells. Possible involvement of phosphorylation of XXXX and reorganization of actin cytoskeleton.

@entity6722 :: ('MESH:C565534', 'Disease') :: ['chemotaxis'] @entity12386 :: ('1072', 'Gene') :: ['cofilin'] @entity21357 :: ('3984', 'Gene') :: ['LIMK1', 'LIM motif-containing kinase', 'LIMK'] @entity224 :: ('MESH:D013481', 'Chemical') :: ['superoxide'] @entity69394 :: ('p|SUB|D|460|A', 'ProteinMutation') :: ['D460A'] @entity18523 :: ('MESH:C034657', 'Chemical') :: ['Texas Red']
@entity12386 :: (1072,Gene) :: ['cofilin']
