BACKGROUND: Bacterial attachment plays an important role in the initiation of @entity253 formation and stent blockage. In vitro studies were conducted to determine the effects of adherence factors, namely pili and glycocalyx production, and culture media, including brain heart infusion broth, modified Vogel and Bonner medium, and @entity1 bile, on the adherence of @entity526 to plastic stents. METHODS: Clinical isolates of @entity526 with different adherence mechanisms, that is, piliated (P+) or nonpiliated (P-), glycocalyx producing (G+) and nonglycocalyx producing (G-), were obtained from clogged stents. Adherence studies were conducted by using the modified Robbins device, and stents were removed at regular intervals to determine the number of attached bacteria/cm(2) with the viable plate count method. @entity691 stents were used to compare the adherence curves of @entity526 with different adherence factors in brain heart infusion broth. The effects of different culture media on the adherence of P+G+ @entity526 to @entity691 stents were determined. In addition, the adherence of P+G+ @entity526 to different plastics in brain heart infusion broth and @entity1 bile was compared. RESULTS: P+G+ @entity526 adhered better than P-G+ and P-G- @entity526 to @entity691 stents. Modified Vogel and Bonner medium, which stimulates glycocalyx production, enhanced the attachment of P+G+ @entity526 , whereas @entity1 bile decreased @entity526 attachment to @entity691 stents, despite an increase in glycocalyx production. There was a difference in adherence of P+G+ @entity526 to @entity691 , @entity2636 , and Teflon stents in brain heart infusion broth, but the differences were nullified in the presence of @entity1 bile. CONCLUSIONS: P+G+ @entity526 with both adherence factors adhere best to plastic stents. Media such as modified Vogel and Bonner medium that stimulate glycocalyx production also enhance bacterial attachment. The toxic effects of @entity3645 in @entity1 bile on the bacteria might alter the adherence mechanism and reduce @entity526 attachment.

Effects of adherence factors and XXXX bile on bacterial attachment and biliary stent blockage: an in vitro study.

@entity1 :: ('9606', 'Species') :: ['human'] @entity691 :: ('MESH:D020959', 'Chemical') :: ['Polyethylene', 'polyethylene'] @entity253 :: ('MESH:D001660', 'Disease') :: ['biliary sludge'] @entity3645 :: ('MESH:D001647', 'Chemical') :: ['bile salts'] @entity2636 :: ('MESH:D011140', 'Chemical') :: ['polyurethane'] @entity526 :: ('562', 'Species') :: ['Escherichia coli', 'E coli']
@entity1 :: (9606,Species) :: ['human']
