BACKGROUND: Cellular function, cell viability and the cytokine network of @entity1 monocytes are influenced by the specific composition of peritoneal dialysis (PD) fluids. In an in vitro study using isolated @entity1 blood monocytes, we investigated the effect of peritoneal dialysates containing @entity1522 ( @entity1472 ) or @entity413 polymer ( @entity367 -poly) instead of @entity413 ( @entity367 ) as the osmotic agent, and @entity3157 (Bic) or PBS instead of @entity880 (Lac) as a buffer. METHODS: The following parameters were studied: mitochondrial dehydrogenase activity (using the @entity98 assay), @entity650 and @entity687 release (ELISA) and cellular @entity650 mRNA expression after lipopolysaccharide (LPS) stimulation (using RT-PCR). FACS flow cytometry with annexin V and @entity2667 as markers and fluorescence microscopic methods were used to study the effects of the test fluids on cell @entity39 and apoptosis. RESULTS: @entity367 /Lac pH 5.5 and @entity367 -poly/PBS pH 7.4 both significantly reduced mitochondrial dehydrogenase activity by more than 50% after 60 minutes of incubation (30.5 +/- 7.6%, 42.5 +/- 6.5%, referred to RPMI 1640 as 100%). Amino/Bic and @entity367 /Bic were both superior (Mtt assay > 63%). The rate of @entity39 cells after 15 minutes of incubation measured by FACS was mostly increased with @entity367 /Lac pH 5.5 (29.9 +/- 4.0%). The rate of apoptotic cells, however, was not significantly different between the test solutions. The concentration of @entity650 in the supernatant of stimulated monocytes was highest with @entity367 /Bic (1023 +/- 278 pg/ml) and Amino/Bic (776 +/- 296 pg/ml) an lowest with @entity367 /lac pH 5.5 (46 +/- 22 pg/ml) and @entity367 -poly/PBS (32 +/- 13 pg/ml). @entity687 release from stimulated monocytes showed a similar pattern. @entity367 -poly/PBS showed a suppressive effect on @entity650 mRNA expression (ratio @entity650 /beta-Actin, 0.4 +/- 0.25 vs. RPMI 1.5 +/- 3.6). CONCLUSIONS: @entity3157 buffered solutions both with @entity413 or @entity1522 as osmotic agents were superior when regarding cell metabolism, viability and cytokine release, while @entity880 buffered solutions and @entity367 -poly/PBS showed some reduced biocompatibility pattern for monocytes in vitro.

Effect of alternative peritoneal dialysis solutions on cell viability, apoptosis/ XXXX and cytokine expression in @entity1 monocytes.

@entity1 :: ('9606', 'Species') :: ['human'] @entity650 :: ('3569', 'Gene') :: ['IL-6', 'interleukin (IL)-6'] @entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity1472 :: ('CHEBI:46882', 'Chemical') :: ['Amino'] @entity2667 :: ('MESH:D011419', 'Chemical') :: ['propidium iodide'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrotic', 'necrosis'] @entity3157 :: ('CHEBI:17544', 'Chemical') :: ['bicarbonate', 'Bicarbonate'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity367 :: ('CHEBI:18237', 'Chemical') :: ['Glu'] @entity687 :: ('3576', 'Gene') :: ['IL-8'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity39 :: (MESH:D009336,Disease) :: ['necrosis']
