AIM: Transplantation of mesenchymal stem cells (MSCs) for the treatment of @entity323 ( @entity323 ) is hampered by apoptosis of the transplanted cells. In @entity323 , there is increased oxidative stress and decreased NO in the corpora cavernosa, and reactive @entity26 species (ROS) induce apoptosis of the transplanted cells. In this study we examined whether and how autophagy was involved in ROS-induced apoptosis of MSCs. METHODS: @entity19 C3H10 MSCs were treated with @entity5568 to simulate the high oxidative condition in @entity323 . Cell viability was measured using @entity98 assay. Apoptosis was analyzed by flow cytometry. Apoptosis- and autophagy-related proteins were detected with Western blot assays. Intracellular autophagosome accumulation was studied using transmission electron microscopy. RESULTS: Treatment of MSCs with @entity5568 (50-400  mol/L) inhibited the cell viability in concentration- and time-dependent manners. Furthermore, @entity5568 (300  mol/L) induced apoptosis, as well as activated autophagy in MSCs. Pretreatment with lysosome inhibitor @entity503 (10  mol/L) or PI3K inhibitor @entity2456 (5 mmol/L) significantly enhanced @entity5568 -induced cell death. Pretreatment with @entity14032 inhibitor @entity4287 (10  mol/L) abrogated @entity5568 -induced accumulation of LC3-II, and attenuated @entity5568 -induced reduction of @entity1379 levels in MSCs. CONCLUSION: ROS induce autophagy to counteract apoptosis in MSCs by activation of @entity14032 . Thus, augmentation of autophagy may reduce apoptosis, prolonging MSC survival and improving MSC-based therapeutic efficacy for @entity323 .

ROS activates XXXX -mediated autophagy to counteract apoptosis in @entity19 mesenchymal stem cells in vitro.

@entity5568 :: ('D006861', 'Chemical') :: ['H2O2'] @entity323 :: ('MESH:D007172', 'Disease') :: ['diabetic erectile dysfunction', 'diabetic ED', 'ED'] @entity2456 :: ('MESH:C025946', 'Chemical') :: ['3-methyladenine'] @entity19 :: ('10090', 'Species') :: ['Mouse'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity4287 :: ('MESH:C432165', 'Chemical') :: ['SP600125'] @entity14032 :: ('26419(Tax:10090)', 'Gene') :: ['JNK'] @entity1379 :: ('12043(Tax:10090)', 'Gene') :: ['Bcl-2'] @entity503 :: ('MESH:D002738', 'Chemical') :: ['chloroquine']
@entity14032 :: (26419(Tax:10090),Gene) :: ['JNK']
