Heat-shock proteins of the 70-kD ( @entity2285 ) family are targets of humoral and cellular immune responses following bacterial or parasitic @entity281 , including @entity1526 . In the present study, we measured antibodies in @entity1 sera reactive with @entity2285 s from the cytoplasm (cy- @entity2285 ), mitochondrion (mt- @entity2285 ), and endoplasmic reticulum ( @entity1477 ) of @entity1527 . Of the three hsp70s tested, only @entity1477 detected @entity1527 @entity281 in more than 90% of nontreated (NT) @entity1 , with cy- @entity2285 and @entity15653 detecting only 78% and 25% of NT @entity1 , respectively. Reactivity of leishmanial sera was 77% with cy- @entity2285 , 13% with @entity1477 , and 5% with mt- @entity2285 . Therefore, considering sensitivity and specificity, the best candidate for @entity1527 serodiagnosis is @entity1477 . Combination of @entity1477 with a @entity1527 24-kD flagellar @entity460 binding protein (FCaBP) increased the diagnostic sensitivity from 90% to 97% but increased leishmanial reactivity from 3% to 8%. To determine whether @entity2285 s are useful for discriminating between cured and noncured @entity1 treated with trypanocidal drugs, we tested sera from @entity15296 ( @entity15296 ) @entity1 and cured @entity1 who have positive conventional serology, termed @entity4785 ( @entity4785 ). The cy- @entity2285 and @entity1477 reacted with 74% and 68% of @entity15296 @entity1 sera, respectively, but these antigens did not discriminate @entity15296 from @entity4785 @entity1 (52% and 45% positive, respectively). The mt- @entity2285 was detected by sera from few @entity15296 @entity1 (18%) and no @entity4785 @entity1 . Although individual hsp70s were not useful for determining the effect of trypanocidal drugs on @entity1527 @entity281 in individual @entity1 , the majority of @entity15296 @entity1 sera (70-80%) reacted with two or three of the hsp70s. In contrast, no @entity4785 sera reacted with all three hsp70s, and 40% did not react with any of the hsp70s, indicating that the number of @entity2285 s detected decreases following successful treatment. Considered together, these results show that @entity1477 has potential as a diagnostic antigen and that absence of reactivity to all three @entity2285 s may be indicative of effective treatment.

Human antibody responses to XXXX 70-kD heat-shock proteins.

@entity1 :: ('9606', 'Species') :: ['human', 'patients', 'patient'] @entity1477 :: ('3309', 'Gene') :: ['grp78'] @entity1527 :: ('5693', 'Species') :: ['Trypanosoma cruzi', 'T. cruzi'] @entity2285 :: ('3308', 'Gene') :: ['hsp70'] @entity4785 :: ('MESH:D004213', 'Disease') :: ['TD', 'treated dissociated'] @entity15653 :: ('3313', 'Gene') :: ['mt-hsp70'] @entity1526 :: ('MESH:D014355', 'Disease') :: ["Chagas' disease"] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection', 'infections'] @entity15296 :: ('MESH:D019553', 'Disease') :: ['treated noncured', 'TNC'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium']
@entity1527 :: (5693,Species) :: ['Trypanosoma cruzi']
