BACKGROUND: The Polymerase Chain Reaction (PCR) method can overcome the limitations of conventional methodology. The aim of this study is to evaluate three primer pairs broadly used @entity1075 , F4/R2 and JPF/JPR, for detection of Brucella by PCR, in @entity1 and animal serum samples and to determine analytic sensitivity of primers. METHODS: Total of 68 serum samples were collected during the acute phase of @entity3377 . 10-fold serial dilutions were prepared from bacterial suspension and serum suspension using @entity61406 . DNA was isolated using boiling. The best dilution from DNA was determined for PCR with three primer pairs. PCR was performed using primer pairs on all bacterial dilutions, serum dilutions, 1/200 dilutions and serum samples. Comparison of sensitivity between three primer pairs was performed with statistical analysis. RESULTS: The best DNA dilution was 1/200. From 68 serum samples, 54 cases (79.41%), 44 cases (64.70%) and 35 cases (51.47%), were positive by PCR with @entity1075 , F4/R2 and JPF/JPR respectively. @entity1075 , F4/R2 and JPF/JPR were able to identify 9 * 10(2), 9 and 9 * 10(5) bacteria in 1 ml of bacterial suspension and 9 * 10(4), 9 * 10(5) and 9 * 10(7) bacteria in 1 ml of dilution 1/200 of Serum dilutions respectively. The differences between primers by statistical analysis were significant for @entity1 , animal and total samples. CONCLUSION: No band was observed in dilutions one of DNA isolated from serum. Therefore, to decrease the effects of inhibitors, DNA was diluted. When DNA isolation is boiling, @entity1075 have the greatest sensitivity for purified bacteria and serum in the detection of Brucella respectively. DNA isolation by boiling can decrease the PCR costs.

Evaluation of different primers for detection of Brucella in XXXX and animal serum samples by using PCR method.

@entity1 :: ('9606', 'Species') :: ['human'] @entity3377 :: ('MESH:D002006', 'Disease') :: ['brucellosis'] @entity61406 :: ('430066', 'Species') :: ['Brucella abortus S19'] @entity1075 :: ('930', 'Gene') :: ['F4/R2 and B4/B5', 'B4/B5']
@entity1 :: (9606,Species) :: ['human']
