Polar organic solvents, such as @entity37409 ( @entity37410 ), @entity6442 , and @entity3733 , have been demonstrated to induce differentiation in a number of neoplastic cell lines, including @entity1 @entity694 cells. Although the mechanism of action of these agents is yet unknown, one possibility is that polar solvents induce a change in lateral mobility of membrane lipids, important to the maturational process. To determine the relationship between polar solvent treatment and changes in membranes, we examined the effects of exposure to @entity37410 on membrane fluidity in @entity1 @entity694 cells (DLD-1; clone A). Membrane viscosity was assessed by determining lipid lateral diffusion following photobleaching of a fluorescent lipid probe in individual intact cells. Exposure of cells to @entity37410 led to a significant @entity101 following 2 days of treatment, with maximal changes occurring after 11 days. @entity37410 induced these effects over a limited concentration range with 1.0% @entity37410 in the medium having the maximal effect, and 0.5% or 1.5% having less or no effect. Growth of cells with @entity6442 (0.8%) also led to increases in membrane viscosity. The observed membrane changes correlated well with the effect of @entity37410 on differentiation in these cells as previously reported, as well as with cell growth rate and morphology in the present study. The increase in viscosity caused by prolonged @entity37410 treatment was reversible, with a return to untreated levels by 9-11 days after removal of @entity37410 . Thus, there is a strong correlation between the attainment of more benign, better differentiated phenotype in polar solvent-treated clone A cells and increases in membrane viscosity.

Polar solvent-induced changes in membrane lipid lateral diffusion in @entity1 XXXX cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity37410 :: ('CHEBI:7438', 'Chemical') :: ['NMF'] @entity37409 :: ('MESH:C002950', 'Chemical') :: ['N-methylformamide'] @entity101 :: ('MESH:D006973', 'Disease') :: ['increase in membrane viscosity'] @entity3733 :: ('MESH:D004121', 'Chemical') :: ['dimethyl sulfoxide'] @entity6442 :: ('MESH:D004126', 'Chemical') :: ['N,N-dimethylformamide'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon cancer']
@entity694 :: (MESH:D003110,Disease) :: ['colon cancer']
