UNASSIGNED: Mesenchymal stromal cells (MSCs) are immunomodulatory and tissue homeostatic cells that have shown beneficial effects in @entity8 and transplantation. Perivascular stromal cells (PSCs) identified within several different organs share characteristics of bone marrow-derived MSCs (BM-MSCs). These PSCs may also possess tissue-specific properties and play a role in local tissue homeostasis. We hypothesized that @entity1 kidney-derived PSCs (hkPSCs) would elicit improved kidney repair in comparison with BM-MSCs. Here we introduce a novel, clinical-grade isolation method of hkPSCs from cadaveric kidneys by enriching for the perivascular marker, @entity28557 . hkPSCs show strong transcriptional similarities to BM-MSCs but also show organotypic expression signatures, including the @entity47114 and @entity33455 nephrogenic transcription factors. Comparable to BM-MSCs, hkPSCs showed immunosuppressive potential and, when cocultured with endothelial cells, vascular plexus formation was supported, which was specifically in the hkPSCs accompanied by an increased @entity28557 expression. hkPSCs did not undergo myofibroblast transformation after exposure to transforming growth factor-b, further corroborating their potential regulatory role in tissue homeostasis. This was further supported by the observation that hkPSCs induced accelerated repair in a tubular epithelial wound scratch assay, which was mediated through hepatocyte growth factor release. In vivo, in a neonatal kidney injection model, hkPSCs reintegrated and survived in the interstitial compartment, whereas BM-MSCs did not show this potential. Moreover, hkPSCs gave protection against the development of @entity810 in vivo in a model of @entity5925 -mediated nephrotoxicity. Overall, this suggests a superior therapeutic potential for the use of hkPSCs and their secretome in the treatment of @entity8 . Stem Cells Translational Medicine 2017;6:405-418.

Clinical-Grade Isolated XXXX Kidney Perivascular Stromal Cells as an Organotypic Cell Source for Kidney Regenerative Medicine.

@entity1 :: ('9606', 'Species') :: ['human'] @entity33455 :: ('3237', 'Gene') :: ['HoxD11'] @entity8 :: ('MESH:D007674', 'Disease') :: ['kidney diseases'] @entity5925 :: ('MESH:D012206', 'Disease') :: ['rhabdomyolysis'] @entity810 :: ('MESH:D058186', 'Disease') :: ['acute kidney injury'] @entity28557 :: ('1464', 'Gene') :: ['NG2'] @entity47114 :: ('3236', 'Gene') :: ['HoxD10']
@entity1 :: (9606,Species) :: ['Human']
