The objective of this study was to assess and compare the chromosome abnormalities present in sporadic and primary sclerosing @entity991 ( @entity201 ) and @entity253 . Histologic sections from 22 @entity1 with CCA (16 sporadic and 6 @entity991 associated), 5 of whom had associated @entity1141 , and 2 @entity991 @entity1 with @entity253 alone were assessed for chromosomal alterations with fluorescence in situ hybridization (FISH). FISH involved the use of a multiprobe set consisting of centromere-specific probes for chromosomes 3, 7, and 17 and a locus-specific probe for 9p21. The number of signals for each of these probes was enumerated in 50 nonoverlapping interphase nuclei, and the percentage of nuclei containing 0, 1, 2, and 3 or more signals was recorded for each probe. @entity986 expression was assessed using immunohistochemistry. Gain of at least 1 chromosome was identified in 19 of 22 (86%) invasive @entity5 and in 4 of 7 (57%) @entity253 . Gain of 2 or more chromosomes (polysomy) was observed in 17 of 22 (77%) invasive @entity5 and in 3 of 7 (43%) @entity253 . Homozygous loss of 9p21 was identified in 11 of 22 (50%) invasive @entity5 and in 3 of 7 (43%) @entity253 . The patterns of chromosomal abnormalities detected by FISH in @entity991 -associated and sporadic @entity201 were similar. Nine of 13 (69%) invasive @entity5 and 2 of 5 (40%) @entity253 with complete loss of @entity986 expression by immunohistochemistry showed allelic loss of 9p21 by FISH. Polysomy and homozygous 9p21 deletion are common in both sporadic and @entity991 -associated @entity201 and are frequently detectable in @entity991 -associated @entity253 .

An assessment of chromosomal alterations detected by fluorescence in situ hybridization and @entity986 expression in sporadic and @entity991 -associated XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity986 :: ('1029', 'Gene') :: ['p16'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumors'] @entity991 :: ('MESH:D015209', 'Disease') :: ['PSC', 'cholangitis (PSC)-associated cholangiocarcinomas'] @entity201 :: ('MESH:D018281', 'Disease') :: ['CCAs'] @entity253 :: ('MESH:D001660', 'Disease') :: ['biliary dysplasias', 'biliary dysplasia'] @entity1141 :: ('MESH:C535662', 'Disease') :: ['dysplasia']
@entity201 :: (MESH:D018281,Disease) :: ['cholangiocarcinomas']
