BACKGROUND: Neurotransmitters had progressive effects on various @entity5 via their different type of receptors. OBJECTIVE: This study was conducted to determine the pattern of @entity750 receptors, respectively, 5HTR2A and 5 @entity19476 gene expression in MCF-7 cells and evaluate their selective antagonist effects on them. METHOD: RT-PCR was performed to determine the pattern of @entity750 receptor gene expression in @entity1 @entity0 cell line (MCF-7). MCF-7 cells were cultured and treated via different doses of tropisetron (5HTR3A antagonist) and @entity6009 (5HTR2A antagonist) for 48 hours. Oxidative and reductive enzyme activity was carried out by @entity98 assay. Subsequently, nuclear morphology of cells was observed by mixed dye florescent staining. To validate cell proliferation inhibition, Real time PCR was carried out for determining the descending rate of @entity1364 ( @entity1364 ) gene expression in treating MCF-7 cells. Assessment of quantification of apoptosis and its discrimination with @entity39 at single cell level using Flowcytometry technique was performed. RESULTS: Results showed that 5HTR2A and 5 @entity19476 have expression in MCF-7 cells. Based on our finding, tropisetron and @entity6009 had suppression effects on MCF-7 cells proliferation. (93.35% in tropisetron 50   moll-1 and 72.36% in @entity6009 25  moll-1 concentration). CONCLUSION: Therefore, the use of tropisetron and @entity6009 as an antagonist of @entity750 receptor may be as new approaches are recommended for the treatment of @entity0 cells.

Alternative Viewpoint against @entity0 Based on Selective XXXX Receptors 5HTR3A and 5HTR2A Antagonists that can mediate Apoptosis in MCF-7 Cell Line.

@entity1 :: ('9606', 'Species') :: ['human'] @entity19476 :: ('3359', 'Gene') :: ['HTR3A'] @entity1364 :: ('5111', 'Gene') :: ['proliferating cell nuclear antigen', 'PCNA'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancers'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity750 :: ('MESH:D012701', 'Chemical') :: ['serotonin'] @entity6009 :: ('MESH:D007650', 'Chemical') :: ['Ketanserin', 'ketanserin']
@entity750 :: (MESH:D012701,Chemical) :: ['Serotonin']
