This study was designed to evaluate the effect of active immunization using recombinant @entity1176 -I protein on reproductive function in @entity235 . Six male and six female @entity235 were randomly assigned to either a control group or an immunization group (n  =  3 males or 3 females/group). @entity235 (aged 16  weeks) were immunized against @entity1176 -I with a @entity7969 -binding protein-gonadotropin-releasing hormone I hexamer generated by recombinant DNA technology. Blood samples were taken at 4-week intervals after immunization. The serum concentrations of @entity251 and @entity53 and anti- @entity1176 -I antibodies were determined by RIA and ELISA, respectively. The results showed that active immunization with recombinant @entity1176 -I increased the serum levels of anti- @entity1176 antibodies (P  <  0.05) and reduced the serum concentrations of @entity251 (P  <  0.05) and @entity53 (P  <  0.05) as compared with the controls. At 28  weeks of age, testes and ovaries were taken surgically for morphologic evaluation. Histologic studies performed on testicular and ovarian tissues revealed clear signs of @entity194 in the recombinant @entity1176 -I-immunized @entity235 and a significant reduction (P  <  0.05) in the weights and sizes of paired testes and ovaries in the treated @entity235 . Microscopically, spermatogonia were visible, but no spermatids and spermatozoa were detected in the seminiferous tubules. Neither early antral nor antral follicles were found in the immunized group. These results demonstrate that recombinant @entity1176 -I is an effective immunogen in @entity235 .

Immunization of XXXX with recombinant GnRH-1 suppresses the development of reproductive function.

@entity251 :: ('MESH:D013739', 'Chemical') :: ['testosterone'] @entity7969 :: ('MESH:D008320', 'Chemical') :: ['maltose'] @entity235 :: ('9615', 'Species') :: ['Dogs', 'dogs'] @entity53 :: ('MESH:D004958', 'Chemical') :: ['estradiol'] @entity194 :: ('MESH:D001284', 'Disease') :: ['atrophy'] @entity1176 :: ('MESH:D007987', 'Chemical') :: ['GnRH']
@entity235 :: (9615,Species) :: ['dogs']
