PURPOSE: We investigated whether @entity20157 could modulate the radiation sensitivity of @entity463 ( @entity463 ) cells and which target gene(s) could be involved. METHODS AND MATERIALS: Three @entity1 @entity11 ( @entity11 ) cell lines and normal @entity1 astrocytes were transfected with control microRNA, pre- @entity20157 , or antisense @entity20157 . Real-time PCR (RT-PCR), clonogenic assays, immunofluorescence, and invasion/migration assays were performed. To predict the target(s), bioinformatics analyses using microRNA target databases were performed. RESULTS: Overexpression of @entity20157 increased the radiation sensitivity of all 3 @entity1 @entity11  cell lines and prolonged radiation-induced y- @entity6714 foci formation. Bioinformatics analyses suggested that @entity20157 could be involved in post-transcriptional control of DNA repair, PI3K/ @entity584 , @entity7242 , and JAK/ @entity4104 and the vascular signaling pathway. Western blot analysis validated the fact that @entity20157 downregulated ATM, @entity6545 , @entity7242 , @entity10411 , PI3K- @entity584 , JAK- @entity4104 , @entity7 , @entity7738 , and @entity1476 . Overexpression of @entity20157 inhibited invasion and migration potentials, downregulated @entity19099 and @entity2277 , and upregulated @entity3670 and @entity11178 . CONCLUSIONS: These data demonstrate that @entity20157 potentially controls DNA damage repair via the PI3K/ @entity584 and JAK/ @entity4104 pathways and may collectively contribute to the modulation of radiation sensitivity in @entity11  cells by inhibiting DNA damage repair, prosurvival signaling, and epithelium-mesenchyme transition. Taken together, these findings demonstrate that @entity20157 could be a target for overcoming the radiation resistance of @entity463 .

MicroRNA-203 Modulates the Radiation Sensitivity of @entity1 XXXX Cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity11178 :: ('7082', 'Gene') :: ['ZO1'] @entity7738 :: ('3091', 'Gene') :: ['HIF-1a'] @entity7242 :: ('6714', 'Gene') :: ['SRC'] @entity7 :: ('7422', 'Gene') :: ['VEGF'] @entity1476 :: ('4313', 'Gene') :: ['MMP2'] @entity4104 :: ('6774', 'Gene') :: ['STAT3'] @entity3670 :: ('9076', 'Gene') :: ['Claudin-1'] @entity584 :: ('207', 'Gene') :: ['AKT'] @entity11 :: ('MESH:D005910', 'Disease') :: ['MG', 'malignant glioma'] @entity19099 :: ('6591', 'Gene') :: ['SLUG'] @entity10411 :: ('5338', 'Gene') :: ['PLD2'] @entity2277 :: ('7431', 'Gene') :: ['Vimentin'] @entity6545 :: ('5888', 'Gene') :: ['RAD51'] @entity463 :: ('MESH:D005909', 'Disease') :: ['GBM', 'glioblastoma'] @entity6714 :: ('3014', 'Gene') :: ['H2AX'] @entity20157 :: ('406986', 'Gene') :: ['miR-203']
@entity11 :: (MESH:D005910,Disease) :: ['Malignant Glioma']
