UNASSIGNED: Laboratory investigations often require centrifugation of blood samples for various erythrocyte tests. Although there is a lack of data about the effect of centrifugation at various g force levels on erythrocyte rheological properties. We aimed to investigate the effect of a 10-minute centrifugation at 500, 1000 or 1500g at 15  C of @entity35 , @entity235 , @entity1707 and @entity1 venous (K3- @entity1098 , 1.5mg/ml) blood samples. Hematological parameters, erythrocyte deformability, cell membrane stability, osmotic gradient ektacytometry (osmoscan) and @entity1423 were determined. Hematological and erythrocyte deformability parameters showed interspecies differences, centrifugation caused no significant alterations. Cell membrane stability for @entity1 erythrocytes centrifuged at higher g level showed less decrease in deformability. Osmoscan O min parameter showed slight elevation in @entity235 centrifuged aliquots. Erythrocyte aggregation parameters changed unexpectedly. @entity35 and @entity235 @entity1423 indices significantly dropped in centrifuged aliquots. @entity1707 @entity1423 indices increased significantly after centrifugation. @entity1 @entity1423 was the most stable one among the investigated species. The used centrifugation protocols caused the largest alterations in @entity1423 in a controversial way among the investigated species. On the other hand, erythrocyte deformability parameters were stable, cell membrane stability and osmoscan data show minor shifts.

The effect of centrifugation at various g force levels on rheological properties of @entity35 , @entity235 , @entity1707 and XXXX red blood cells.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity1707 :: ('9823', 'Species') :: ['Pig', 'pig'] @entity1423 :: ('MESH:D001791', 'Disease') :: ['erythrocyte aggregation'] @entity235 :: ('9615', 'Species') :: ['dog'] @entity35 :: ('10116', 'Species') :: ['rat', 'Rat'] @entity1098 :: ('MESH:D004492', 'Chemical') :: ['EDTA']
@entity1 :: (9606,Species) :: ['human']
