UNASSIGNED: In this study, we investigate the effect of short hairpin RNA-mediated gene silencing of @entity3752 expression on chemosensitivity of @entity1759 (+) @entity189 cancer stem-like cells. The sequence-specific short hairpin RNA lentivirus targeting at @entity1 @entity3752 was synthesized and used to infect @entity1759 (+) nasopharyngeal cells that were sorted by flow cytometry. We also employed flow cytometry to detect transfection efficiency. Real-time polymerase chain reaction was used to detect @entity3752 and its downstream repressor genes @entity986 ( @entity986 ) and @entity986 (ARF) messenger RNA, while each protein expression level of @entity3752 , @entity986 ( @entity986 ), @entity986 (ARF), and @entity876 was confirmed by Western blotting protocol. @entity5 spheroid assay was used to evaluate the self-renewal capacity. @entity2454 assay and colony formation assay were applied to detect proliferation capacity and colony-forming capacity under different concentrations of chemotherapeutic drugs @entity1487 or @entity900 . Transwell cell migration and invasion assay were employed to observe migration and invasion capacity after cells were exposed to @entity900 for 24 hours. The constructed short hairpin RNA lentivirus targeting @entity3752 gene successfully infected into the @entity1759 (+) @entity189 cells and effectively inhibited the @entity3752 messenger RNA and protein expression level, while the expression level of Bim-1 target genes, @entity986 ( @entity986 ), @entity986 (ARF), and @entity876 was significantly increased (P < .05). Notably, the proliferation, colony formation, migration, and invasion capabilities of the sequence-specific short hairpin RNA lentivirus-infected @entity1759 (+) @entity189 cells reduced significantly under chemotherapeutic treatments (P < .05). Our results indicated that @entity3752 may play an important role in the chemosensitivity of @entity1759 (+) @entity189 cancer stem-like cells. @entity3752 may be a potential new target for the treatment of @entity189 displaying chemotherapy resistance.

Effect of shRNA-Mediated Gene Silencing of XXXX Expression on Chemosensitivity of @entity1759 + @entity189 Cancer Stem-Like Cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity986 :: ('1029', 'Gene') :: ['p16', 'p14', 'INK4a'] @entity3752 :: ('648', 'Gene') :: ['Bmi-1'] @entity5 :: ('MESH:D009369', 'Disease') :: ['Tumor'] @entity900 :: ('MESH:D002945', 'Chemical') :: ['cisplatin'] @entity189 :: ('MESH:D002277', 'Disease') :: ['nasopharyngeal carcinoma'] @entity1759 :: ('960', 'Gene') :: ['CD44'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity2454 :: ('MESH:C022616', 'Chemical') :: ['3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide'] @entity1487 :: ('MESH:D005472', 'Chemical') :: ['5-fluorouracil']
@entity3752 :: (648,Gene) :: ['Bmi-1']
