UNASSIGNED: The aim of the present study was to elucidate the intracellular mechanisms that cause neuronal cell death following exposure to excitatory neurotransmitter  -induced @entity2359 , @entity2359 and oxidative stress. @entity1 SH  -SY5Y @entity521 cells were exposed to various stimuli, including @entity3264 , @entity2955 ( @entity2955 ), and @entity413 oxidase, and cell viability was determined by @entity98 assay. Early apoptosis and @entity39 were examined by @entity4404 / @entity2667 double staining and flow cytometric analysis. Intracellular @entity460 ion concentration and mitochondrial membrane potential were assessed by Fluo  -3a and JC  -1 staining, respectively. In addition, protein expression of @entity13997 ( @entity13997 ) kinase  1 and @entity13997 kinase  3 were evaluated by western blotting. @entity3264 , @entity2955 and @entity413 oxidase treatment decreased cell viability. @entity3264 induced apoptosis and @entity39 , whereas, @entity2955 induced cell @entity39 and @entity413 oxidase induced apoptosis. Furthermore, @entity3264 , @entity2955 or @entity413 oxidase treatment significantly increased intracellular @entity460 concentrations (P<0.05). The effect of @entity3264 on mitochondrial membrane potential varied with high and low concentrations, whereas @entity2955 and @entity413 oxidase significantly increased the mitochondrial membrane potential in the SH  -SY5Y cells (P<0.05). @entity3264 significantly upregulated expression levels of @entity13997 kinase  1 (P<0.05), but not @entity13997 kinase  3. These findings demonstrate that the response of SH  -SY5Y cells varies with the stimuli. Furthermore, @entity13997 kinase  1 may specifically regulate programmed @entity39 in @entity3264  -mediated excitatory @entity137 , but not in cell damage induced by either @entity2955 or @entity413 oxidase.

Stimulus-dependent neuronal cell responses in SH-SY5Y XXXX cells.

@entity1 :: ('9606', 'Species') :: ['Human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity3264 :: ('D018698', 'Chemical') :: ['glutamate', 'Glutamate'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis'] @entity4404 :: ('308', 'Gene') :: ['Annexin  V'] @entity2667 :: ('MESH:D011419', 'Chemical') :: ['propidium iodide'] @entity13997 :: ('MESH:C563663', 'Disease') :: ['receptor  -interacting protein', 'RIP'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity521 :: ('MESH:D009447', 'Disease') :: ['neuroblastoma'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity2955 :: ('MESH:D016627', 'Chemical') :: ['6  -OHDA', '6-OHDA', '6  -hydroxydopamine'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity2359 :: ('MESH:D020258', 'Disease') :: ['neurotoxicity', 'neurotoxins']
@entity521 :: (MESH:D009447,Disease) :: ['neuroblastoma']
