@entity35 liver 60 S ribosomal subunits were modified with @entity36991 . After treatment with @entity273 , the cross-linked proteins were extracted and then separated into 24 fractions by chromatography on carboxymethylcellulose. Each protein fraction was then analyzed by diagonal @entity2975 - @entity3208 gel electrophoresis (Sommer, A., and Traut, R.R. (1974) Proc. Natl. Acad. Sci. U. S. A. 71, 3946-3950). The pieces of gel containing cross-linked protein spots that were shifted from the diagonal line were labeled with 125I. The labeled protein was extracted from the gel and identified by three kinds of two-dimensional gel electrophoresis, followed by autoradiography. Fifty-three @entity2915 involving 35 protein species containing two acidic proteins were identified. From these and previous results, a preliminary model of the protein topography of the 60 S ribosomal subunit was constructed and discussed in relation to other functional data on 60 S ribosomal proteins.

Cross-linking study on protein topography of XXXX liver 60 S ribosomal subunits with @entity36991 .

@entity3208 :: ('MESH:D012967', 'Chemical') :: ['sodium dodecyl sulfate'] @entity36991 :: ('MESH:C005905', 'Chemical') :: ['2-iminothiolane'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['hydrogen peroxide'] @entity2915 :: ('MESH:D006948', 'Disease') :: ['cross-linked protein pairs'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity35 :: ('10116', 'Species') :: ['Rat']
@entity35 :: (10116,Species) :: ['rat']
