PURPOSE: The aim of the study was to investigate the effect of @entity205 ( @entity9472 ) on @entity1810 / @entity15927 signaling in @entity35 gingival fibroblasts and to explore the mechanism of @entity9472 induced @entity8032 . METHODS: Healthy @entity35 gingival fibroblasts were cultured with different concentrations of @entity9472 and the cell proliferations were assessed with CCK-8 assay. The mRNA levels of @entity1810 , @entity15927 and collagen I were measured by real-time PCR. The protein level of @entity1810 , @entity15927 , p- @entity15927 and collagen I were determined using western blot and immumofluorescence. Cell migration ability was detected by cell wound scratch assay. The data was analyzed with SPSS 20.0 software package. RESULTS: The use of 200 ng/mL @entity9472 stimulated proliferation and @entity2522 . The mRNA levels of @entity1810 and collagen I were significantly promoted after @entity9472 exposure. The protein syntheses of @entity1810 , p- @entity15927 and Collagen I were also increased by @entity9472 stimulation. CONCLUSIONS: @entity9472 may activate @entity1810 / @entity15927 signaling pathway, thus promoting the proliferation and migration of @entity35 gingival fibroblasts as well as collagen accumulation, which eventually lead to @entity8032 .

[Effect of @entity205 on XXXX / @entity15927 signaling in @entity35 gingival fibroblasts].

@entity9472 :: ('D016572', 'Chemical') :: ['CsA'] @entity15927 :: ('25631(Tax:10116)', 'Gene') :: ['Smad3'] @entity2522 :: ('MESH:D005891', 'Disease') :: ['migration of gingival fibroblasts'] @entity205 :: ('MESH:D016572', 'Chemical') :: ['cyclosporine A'] @entity1810 :: ('59086(Tax:10116)', 'Gene') :: ['TGF-b', 'TGF-b1'] @entity35 :: ('10116', 'Species') :: ['rat', 'Sprague-Dawley rat'] @entity8032 :: ('MESH:D019214', 'Disease') :: ['gingival overgrowth']
@entity1810 :: (59086(Tax:10116),Gene) :: ['TGF-b']
