OBJECTIVE: To examine epigenetic events of aberrant promoter methylation as diagnostic markers in primary @entity213 using a novel multigene approach. Promoter methylation-mediated silencing is a hallmark of several established @entity5 suppressor genes. Changes in DNA methylation have been reported to occur early in @entity105 and therefore are potentially important early indicators of existing disease. DESIGN: A multicandidate gene probe panel interrogated DNA for aberrant methylation status in 22 @entity5 genes using the methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) assay. Aberrant promoter hypermethylation was confirmed using methylation-specific polymerase chain reaction after @entity9736 treatment. SETTING: Primary care medical center. SUBJECTS: We examined fresh-frozen primary @entity135 specimens from 28 @entity1 , including 21 late-stage (19 stage IV and 2 stage III) and 7 early-stage (6 stage II and 1 stage I) @entity5 . RESULTS: Promoter hypermethylation was observed in 14 of the 28 @entity1 (50%). Genes for @entity4882 , @entity1412 , and @entity10428 were most frequently hypermethylated, occurring in 11 (39%) for @entity4882 , 7 (25%) for @entity10428 , and 6 (21%) for @entity1412 . Aberrant methylation of @entity10428 was solely a stage IV event. Methylation-specific polymerase chain reaction after @entity9736 treatment with conventional and real-time polymerase chain reaction confirmed aberrant methylation for @entity4882 and @entity10428 . CONCLUSIONS: Promoter methylation profiling of primary @entity213 using multiple target genes identified @entity4882 , @entity1412 , and @entity10428 as frequent epigenetic events. The clinical implications of these genes as diagnostic and treatment biomarkers are highly relevant as attractive targets for @entity5 therapy, given the reversible nature of epigenetic gene silencing.

Methylation of multiple genes as diagnostic and therapeutic markers in primary XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity135 :: ('MESH:D006258', 'Disease') :: ['head and neck tumor'] @entity10428 :: ('55743', 'Gene') :: ['CHFR'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'tumors'] @entity1412 :: ('MESH:D011125', 'Disease') :: ['APC'] @entity9736 :: ('MESH:C042345', 'Chemical') :: ['bisulfite'] @entity4882 :: ('5915', 'Gene') :: ['RARB'] @entity105 :: ('MESH:D063646', 'Disease') :: ['carcinogenesis'] @entity213 :: ('MESH:C535575', 'Disease') :: ['head and neck squamous cell carcinoma']
@entity213 :: (MESH:C535575,Disease) :: ['head and neck squamous cell carcinoma']
