Integrin-associated intracellular @entity141 oscillations modulate cell migration, probably by controlling integrin-mediated release of the cell rear during migration. @entity3818 ( @entity3818 ), via its @entity669 phosphorylation activity, plays a key role in integrin signaling. In @entity1 U87 @entity816 cells, expression of the dominant negative @entity3818 -related non-kinase domain (FRNK) inhibits the @entity141 -sensitive component of serum-dependent migration. We investigated how integrin-associated @entity141 signaling might be coupled to focal adhesion (FA) dynamics by visualizing the effects of @entity141 spikes on FAs using green fluorescent protein (GFP)-tagged @entity3818 and FRNK. We report that @entity141 spikes are temporally correlated with movement and disassembly of FAs, but not their formation. FRNK transfection did not affect generation of @entity141 spikes, although cell morphology was altered, with fewer FAs of larger size and having a more peripheral localization being observed. Larger sized FAs in FRNK-transfected cells were not disassembled by @entity141 spikes, providing a possible explanation for impaired @entity141 -dependent migration in these cells. Stress fiber end movements initiated by @entity141 spikes were visualized using GFP-tagged @entity19077 ( @entity19077 ). @entity141 -associated movements of stress fiber ends and FAs had similar kinetics, suggesting that stress fibers and FAs move in a coordinated fashion. This indicates that increases in @entity141 likely trigger disassembly of adhesive structures that involves disruption of integrin-extracellular matrix interactions, supporting a key role for @entity141 -sensitive inside-out signaling in cell migration. A rapid increase in @entity669 phosphorylation of @entity3818 was found in response to an elevation in @entity141 induced by @entity2426 , and we propose that this represents the initial triggering event linking @entity141 signaling and FA dynamics to cell motility.

Calcium oscillations trigger focal adhesion disassembly in @entity1 U87 XXXX cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity2426 :: ('MESH:D019284', 'Chemical') :: ['thapsigargin'] @entity19077 :: ('4638', 'Gene') :: ['myosin light chain kinase', 'MLCK'] @entity816 :: ('MESH:D001254', 'Disease') :: ['astrocytoma'] @entity3818 :: ('5747', 'Gene') :: ['FAK', 'Focal adhesion kinase'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine']
@entity816 :: (MESH:D001254,Disease) :: ['astrocytoma']
