Gordonia sp. C-6 can desulfurize @entity70238 (BT) as the pathway similar to the "4S" pathway of @entity31422 ( @entity20207 )-desulfurizing, but the strain can not grow with @entity20207 as the sole @entity599 source. At current, there were not related reports on BT-desulfurizing genes at home or abroad. The @entity20207 -desulfurizing genes of Rhodocossus erythropolis DS-3, dszA, dszB, dszC and dszABC were introduced into Gordonia sp. C-6 respectively using a Rhodococcus- @entity526 shuttle vector, to construct new recombinant strains Gordonia sp. CRA, Gordonia sp. @entity11103 , Gordonia sp. @entity14 and Gordonia sp. CRABC, the enzyme activities of which was respectively 76.8 micromol x (g x h)(-1), 51.6 micromol x (g x h)(-1) and 62.4 micromol x (g x h)(-1), increasing by 1.5 times compared with 35.2 micromol x (g x h)(-1), 21.3 micromol x (g x h)(-1) and 25.5 micromol x (g x h)(-1) of wild strain Rhodocossus erythropolis DS-3. Of the recombinant strains, only recombinant strains Gordonia sp. @entity14 with DszC and Gordonia sp. CRABC with DszABC exhibite significant growth with @entity20207 as the sole @entity599 source, Gordonia sp. CRA with DszA and Gordonia sp. @entity11103 with DszB could not live with @entity20207 as the sole @entity599 source. The results show that, @entity20207 monoxygenase and BT monoxygenase, catalyzed the first two steps of @entity20207 and BT oxidation, respectively, are the key enzymes responsible for substrate-recognition, however, the enzymes catalyzed the last two steps have the similar substrate specificity. The active sites of the two monoxygenase could be predicted by comparing with the sequence differences of their @entity1522 .

[Functional correlation of XXXX and @entity70238 desulfurization enzymes].

@entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity31422 :: ('MESH:C016366', 'Chemical') :: ['dibenzothiophene'] @entity11103 :: ('MESH:C536600', 'Disease') :: ['CRB'] @entity14 :: ('MESH:D015179', 'Disease') :: ['CRC'] @entity599 :: ('MESH:D013455', 'Chemical') :: ['sulfur'] @entity20207 :: ('CHEBI:23686', 'Chemical') :: ['DBT'] @entity70238 :: ('MESH:C088015', 'Chemical') :: ['benzothiophene'] @entity526 :: ('562', 'Species') :: ['E. coli']
@entity31422 :: (MESH:C016366,Chemical) :: ['dibenzothiophene']
