The action of @entity15258 ( @entity15258 ) on @entity141 signalling in HeLa cells and cardiac myocytes was investigated. Consistent with other studies, we found that superfusion of cells with @entity15258 rapidly inhibited @entity671 (InsP(3))-mediated @entity141 release and store-operated @entity141 entry (SOC). In addition to abrogating hormone-evoked @entity141 responses, @entity15258 could antagonise @entity141 signals evoked by a membrane permeant InsP(3) @entity13507 . @entity15258 also slowed the recovery of intracellular @entity141 signals consistent with an effect on @entity141 ATPases. The inhibitory action of @entity15258 on InsP(3) receptors (InsP(3)Rs), SOC channels and @entity141 pumps persisted for several minutes after washout of the compound. Application of @entity15258 to unstimulated cells had no effect on subsequent @entity141 responses suggesting that it has a use-dependent action. Mitochondria in cells treated with @entity15258 showed a rapid and slowly reversible swelling. @entity15258 did not cause the mitochondria to depolarise, but it reduced the extent of mitochondrial @entity460 uptake. Although @entity15258 has been demonstrated not to affect voltage-operated @entity141 channels or @entity8452 receptors, we found that it gave a concentration-dependent long-lasting inhibition of @entity141 signalling in electrically-stimulated cardiac myocytes, where InsP(3)Rs and SOC channels do not play a significant role. Our data suggest that @entity15258 has multiple cellular targets, a use-dependent action, is difficult to reverse and may affect @entity141 signalling in cell types where InsP(3) and SOC are not active.

@entity15258 ( @entity15258 ) antagonises @entity671 -induced XXXX release, inhibits @entity460 pumps and has a use-dependent and slowly reversible action on store-operated @entity460 entry channels.

@entity671 :: ('MESH:D015544', 'Chemical') :: ['inositol 1,4,5-trisphosphate'] @entity8452 :: ('MESH:D012433', 'Chemical') :: ['ryanodine'] @entity15258 :: ('MESH:C109986', 'Chemical') :: ['2-APB', '2-aminoethoxydiphenyl borate'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity13507 :: ('CHEBI:35701', 'Chemical') :: ['ester']
@entity460 :: (MESH:D002118,Chemical) :: ['calcium']
