This study aimed to isolate and evaluate the cellulase activity of cellulolytic bacteria in hot springs of Dehloran, Ilam province, Iran. Water and sludge samples were collected from the hot springs and the bacterial enrichment was performed in a medium containing @entity579 barn and @entity5070 cellulose (CMC). The cultures were incubated at 50   C in aerobic conditions. The bacteria were isolated on CMC agar (1%) medium. Cellulase assay of the isolates was measured by the evaluation of endoglucanase enzyme activity, which is also called as @entity5070 cellulase (CMCase). The isolated thermotolerant bacteria were then identified and optimized for the production of CMCase. Moreover, stabilizing elements of the enzyme were identified with in silico approach. The chosen isolate was identified as @entity69380 sp. IDAH9. The identified strain produced the most thermostable CMCase at a concentration of 5.6 g/L of @entity1329 , 9 g/L CMCase or 12 g/L @entity579 bran, 0/6% @entity9266 , and 0.2% @entity969 . The produced enzyme showed 80% of the residual activity after 1 h of incubation at 65   C. In silico data indicated that the remaining residual activity was due to the redundant stabilizing elements in the protein structure. Consequently, @entity69380 can be isolated from the extreme environment and has a thermostable endoglucanase which may be used for various applications after studying them.

Characterization of a thermostable endoglucanase produced by XXXX sp. IDAH9.

@entity9266 :: ('MESH:D011136', 'Chemical') :: ['Tween-80'] @entity69380 :: ('139208', 'Species') :: ['Isoptericola variabilis', 'I. variabilis'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity5070 :: ('CHEBI:41402', 'Chemical') :: ['carboxymethyl'] @entity1329 :: ('MESH:D000645', 'Chemical') :: ['ammonium sulfate'] @entity579 :: ('4530', 'Species') :: ['rice']
@entity69380 :: (139208,Species) :: ['Isoptericola variabilis']
