BACKGROUND: Egg white proteins are usually subjected to heating, making them edible for the majority of @entity1 with egg @entity566 . OBJECTIVE: We sought to investigate the underlying mechanisms responsible for the reduced allergenicity displayed by heat-treated egg white allergens. METHODS: C3H/HeJ @entity19 were orally sensitized with @entity10328 (OVA) or ovomucoid and challenged with native or heated proteins to evaluate their allergenicity. Immunoreactivity was assessed by immunoblotting using sera from @entity1 with egg @entity566 . In vitro gastrointestinal digestion of native and heated OVA and ovomucoid was studied by @entity214 -PAGE and liquid chromatography. Intestinal uptake of intact native and heated OVA and ovomucoid by @entity1 intestinal epithelial (Caco-2) cells was investigated. @entity35 basophil @entity1778 cells passively sensitized with mouse serum and @entity1 basophils passively sensitized with serum from @entity1 with egg @entity566 were used to assess the effector cell activation by heated, digested, and transported OVA and ovomucoid. RESULTS: Heated OVA and ovomucoid did not induce symptoms of @entity2021 in sensitized @entity19 when administered orally. Heating did not completely destroy IgE-binding capacity of OVA or ovomucoid but enhanced in vitro digestibility of OVA. Digestion of both OVA and ovomucoid diminished mediator release in @entity35 basophil @entity1778 assay and basophil activation. Heating of allergens prevented transport across @entity1 intestinal epithelial cells in a form capable of triggering basophil activation or T-cell activation. CONCLUSION: Heat treatment reduces allergenicity of OVA and ovomucoid. This is partially a result of the enhanced gastrointestinal digestibility of heated OVA and the inability of heated OVA or ovomucoid to be absorbed in a form capable of triggering basophils.

Mechanisms underlying differential food XXXX response to heated egg.

@entity1 :: ('9606', 'Species') :: ['children', 'human'] @entity10328 :: ('282665(Tax:10090)', 'Gene') :: ['ovalbumin'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity566 :: ('MESH:D006967', 'Disease') :: ['allergy'] @entity1778 :: ('MESH:D007938', 'Disease') :: ['leukemia'] @entity35 :: ('10116', 'Species') :: ['rat', 'Rat'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS'] @entity2021 :: ('MESH:D000707', 'Disease') :: ['anaphylaxis']
@entity566 :: (MESH:D006967,Disease) :: ['allergy']
