UNASSIGNED: Subcellular @entity20188 biosynthesis capacity based on local @entity68871 ( @entity3006 ) availability or locally boosted @entity3006 production was determined for plastids, cytosol and mitochondria. A @entity4661 synthase (GES) was targeted to plastids, cytosol, or mitochondria. Transient expression in Nicotiana benthamiana indicated local @entity3006 availability for each compartment but resulted in different product levels. A @entity3006 synthase from Picea abies (PaGDPS1) was shown to boost @entity3006 production. PaGDPS1 was also targeted to plastids, cytosol or mitochondria and PaGDPS1 and GES were coexpressed in all possible combinations. @entity4661 and @entity4661 -derived products were analyzed by GC-MS and LC-MS, respectively. GES product levels were highest for plastid-targeted GES, followed by mitochondrial- and then cytosolic-targeted GES. For each compartment local boosting of @entity3006 biosynthesis increased GES product levels. @entity3006 exchange between compartments is not equal: while no @entity3006 is exchanged from the cytosol to the plastids, 100% of @entity3006 in mitochondria can be exchanged to plastids, while only 7% of @entity3006 from plastids is available for mitochondria. This suggests a direct exchange mechanism for @entity3006 between plastids and mitochondria. Cytosolic PaGDPS1 competes with plastidial GES activity, suggesting an effective drain of @entity23159 from the plastids to the cytosol.

XXXX biosynthesis potential of plant subcellular compartments.

@entity20188 :: ('CHEBI:35187', 'Chemical') :: ['monoterpene'] @entity23159 :: ('MESH:C004809', 'Chemical') :: ['isopentenyl diphosphate'] @entity3006 :: ('MESH:D006153', 'Chemical') :: ['GDP'] @entity4661 :: ('MESH:C007836', 'Chemical') :: ['geraniol', 'Geraniol'] @entity68871 :: ('MESH:C511282', 'Chemical') :: ['geranyl diphosphate']
@entity20188 :: (CHEBI:35187,Chemical) :: ['Monoterpene']
