PURPOSE: Ten eleven translocation (TET) enzyme activity is essential for active DNA demethylation in biological processes, and their altered expression has been observed in various @entity5 . Therefore, we investigated DNA methylation and mRNA levels of all TETs in @entity14 ( @entity14 ) @entity1 . METHODS: TET mRNA levels were evaluated using quantitative RT-PCR in primary @entity5 and histopathologically unchanged colorectal tissues from @entity1 who underwent radical surgical colon resection (n  =  113). DNA methylation levels of the TET CpG island were assessed using bisulfite DNA sequencing and high-resolution melting analysis. RESULTS: We found reduced transcript levels of @entity10279 , @entity5312 and @entity10280 in @entity5 tissue compared with their histopathologically unchanged counterparts (p  =  0.000011; p  =  0.000001; p  =  0.00031, respectively). Importantly, multivariate Cox regression analysis revealed favorable overall survival (OS) and disease-free survival (DFS) outcomes for @entity1 with high @entity5312 mRNA levels in histopathologically unchanged tissue (HR(OS)  =  0.091, 95  % CI 0.011-0.77, p  =  0.028; HR(DFS)  =  0.21, 95  % CI 0.04-1.06, p  =  0.059). Moreover, we found no DNA methylation in the @entity5312 and @entity10280 promoter regions in @entity5 and histopathologically unchanged tissue. In contrast, we reported @entity10279 DNA hypermethylation in a small fraction of @entity1 (n  =  12/113). CONCLUSION: To best of our knowledge, our study is the first to investigate TET mRNA levels in a cohort of @entity14 @entity1 and correlate them with @entity1 ' prognosis. Present study provides the evidence that @entity5312 mRNA expression may be an independent prognostic factor for disease recurrence and outcome. Additionally, our findings initially indicate the importance of DNA methylation in regulating @entity10279 expression.

Clinical significance of DNA methylation mRNA levels of TET family members in XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5312 :: ('54790', 'Gene') :: ['TET2'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancerous', 'malignancies'] @entity10280 :: ('200424', 'Gene') :: ['TET3'] @entity10279 :: ('80312', 'Gene') :: ['TET1'] @entity14 :: ('MESH:D015179', 'Disease') :: ['CRC', 'colorectal cancer']
@entity14 :: (MESH:D015179,Disease) :: ['colorectal cancer']
