Mast cell (MC) degranulation is the foundation of the acute phase of @entity2023 ( @entity2023 ). Previously, downregulation of @entity19986 ( @entity19986 ) was shown to suppress MC activation in an @entity2023 @entity19 model. Binding of microRNA-135a (miR-135a) to @entity19986 was also observed, and overexpression of this miRNA decreased @entity19986 mRNA and protein expression. However, the effects of miR-135a on MCs during @entity2023 are currently unknown. In the present study, we utilized a lentiviral (LV) vector to intranasally administer miR-135a to ovalbumin (OVA)-sensitized @entity2023 @entity19 . Following miR-135a treatment, the total serum IgE concentration observed during @entity2023 was significantly reduced. In the nasal mucosa, the expression of T-box expressed in T cells ( @entity19985 ) was higher, whereas that of @entity19986 was lower in the @entity2023 @entity19 following miRNA treatment. Notably, during @entity2023 , the ratio of type 1 T-helper cells ( @entity630 ) to type 2 ( @entity61 ) cells in the spleen is unbalanced, favoring @entity61 . However, administering miR-135a to the @entity2023 @entity19 appeared to balance this ratio by increasing and decreasing the percentage of @entity630 and @entity61 cells, respectively. MiR-135a also appeared to strongly suppress the infiltration of eosinophils and MCs into the nasal mucosa, and it was specifically localized in the MCs, suggesting that its influence is modulated through regulation of @entity19986 in these cells. Additional work identifying the full therapeutic potential of miR-135a in the treatment of @entity2023 and diseases involving allergen-induced @entity32 is warranted.

Intranasal Administration of Lentiviral miR-135a Regulates Mast Cell and Allergen-Induced @entity32 by Targeting XXXX .

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity2023 :: ('MESH:D006255', 'Disease') :: ['AR', 'allergic rhinitis'] @entity19986 :: ('14462(Tax:10090)', 'Gene') :: ['GATA-3', 'GATA binding protein 3'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity61 :: ('15111(Tax:10090)', 'Gene') :: ['Th2'] @entity19985 :: ('57765(Tax:10090)', 'Gene') :: ['T-bet'] @entity630 :: ('57314(Tax:10090)', 'Gene') :: ['Th1']
@entity19986 :: (14462(Tax:10090),Gene) :: ['GATA-3']
