UNASSIGNED: Various particles such as Alum or @entity3440 are known to act as an adjuvant if co-administered with vaccine antigens. Several reports have demonstrated that the adjuvanticity is strongly affected by the physicochemical properties of particles such as the size, shape and surface charge, although the required properties and its relationship to the adjuvanticity are still controversial. @entity692 particle ( @entity19916 ) composed of @entity78 has been shown to work as adjuvant in @entity19 . However, the properties of @entity19916 required for the adjuvanticity have not been fully characterized yet. In this study, we examined the role of size or shape of HAps in the antibody responses after immunization with antigen. HAps whose diameter ranging between 100 and 400nm provided significantly higher antibody responses than smaller or larger ones. By comparison between sphere and rod shaped HAps, rod shaped HAps induced stronger inflammasome-dependent @entity635 production than the sphere shaped ones in vitro. However, sphere- and rod-shaped @entity19916 elicited comparable antibody response in WT @entity19 . Vice versa, @entity6198 (-/-), @entity34552 (-/-) or @entity13761 (-/-) @entity19 provided comparable level of antibody responses to @entity19916 adjuvanted vaccination. Collectively, our results demonstrated that the size rather than shape is a more critical property, and @entity635 production via @entity6198 inflammasome is dispensable for the adjuvanticity of HAps in @entity19 .

Optimization of physiological properties of XXXX as a vaccine adjuvant.

@entity19 :: ('10090', 'Species') :: ['mice'] @entity635 :: ('16176(Tax:10090)', 'Gene') :: ['IL-1b'] @entity78 :: ('MESH:C020243', 'Chemical') :: ['calcium phosphate'] @entity692 :: ('MESH:D017886', 'Chemical') :: ['Hydroxyapatite'] @entity34552 :: ('20905(Tax:10090)', 'Gene') :: ['Asc'] @entity3440 :: ('MESH:D012822', 'Chemical') :: ['silica'] @entity13761 :: ('12362(Tax:10090)', 'Gene') :: ['Caspase1'] @entity6198 :: ('216799(Tax:10090)', 'Gene') :: ['Nlrp3', 'NLRP3'] @entity19916 :: ('218772(Tax:10090)', 'Gene') :: ['HAp']
@entity692 :: (MESH:D017886,Chemical) :: ['hydroxyapatite']
