@entity5 continues to defy current chemotherapeutic regimens. We tested the garlic compounds @entity12971 ( @entity22770 ) and @entity35864 ( @entity22770 ) for induction of apoptosis in @entity1 malignant @entity521 SH-SY5Y cells. Viability of @entity1 primary neurons was unaffected after 24 h treatment with 50 and 100 microM DAS and 50 microM DADS but slightly affected with 100 microM DADS. Treatment with 50 and 100 microM DAS or DADS significantly decreased viability in SH-SY5Y cells. Wright staining showed morphological features of apoptosis in SH-SY5Y cells treated with 50 and 100 microM DAS or DADS for 24 h. ApopTag assay demonstrated DNA fragmentation in apoptotic cells. Apoptosis was associated with an increase in [ @entity141 ](i), increase in @entity589 : @entity588 ratio, mitochondrial release of @entity4674 , increase in cytosolic @entity17205 / @entity17205 , and down regulation of inhibitor-of-apoptosis proteins and @entity2451 ( @entity2451 ). Activation of @entity8187 and @entity522 indicated involvement of intrinsic pathway of apoptosis. Calpain and @entity522 activities produced 145 kD spectrin break down product (SBDP) and 120 kD SBDP, respectively. Also, @entity522 activity cleaved inhibitor of @entity13779 ( @entity15975 ). Results strongly suggested that the garlic compounds DAS and DADS suppressed anti-apoptotic factors and activated calpain and intrinsic caspase cascade for apoptosis in SH-SY5Y cells.

Garlic compounds induced calpain and intrinsic caspase cascade for apoptosis in @entity1 malignant XXXX SH-SY5Y cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity35864 :: ('MESH:C028009', 'Chemical') :: ['diallyl disulfide'] @entity17205 :: ('56616', 'Gene') :: ['Diablo', 'Smac'] @entity589 :: ('581', 'Gene') :: ['Bax'] @entity588 :: ('596', 'Gene') :: ['Bcl-2'] @entity2451 :: ('4790', 'Gene') :: ['nuclear factor kappa B', 'NFkappaB'] @entity522 :: ('836', 'Gene') :: ['caspase-3'] @entity5 :: ('MESH:D009369', 'Disease') :: ['Malignant (N-type) neuroblastoma'] @entity12971 :: ('MESH:C038491', 'Chemical') :: ['diallyl sulfide'] @entity4674 :: ('54205', 'Gene') :: ['cytochrome c'] @entity22770 :: ('MESH:C025953', 'Chemical') :: ['DAS', 'DADS'] @entity8187 :: ('842', 'Gene') :: ['caspase-9'] @entity15975 :: ('1676', 'Gene') :: ['ICAD'] @entity13779 :: ('1677', 'Gene') :: ['caspase-activated DNase'] @entity521 :: ('MESH:D009447', 'Disease') :: ['neuroblastoma'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)']
@entity521 :: (MESH:D009447,Disease) :: ['neuroblastoma']
