OBJECTIVE: To study cytotoxic effects of @entity4447 ( @entity9188 ) on the @entity1 lens epithelium cells and to identify the biological mechanism for these effects. METHODS: In this experimental study, @entity1 lens epithelium cells (FHL124 cells) were cultured in Eagle's minimum essential medium supplemented with 5% fetal calf serum. The effects of @entity9188 on FHL124 cells growth were tested by @entity98 , and apoptosis was detected by TUNEL assay. Gene changes were detected by real-time PCR (Taqman). @entity9188 -induced changes in cell @entity460 level were measured by real-time fluorometric single-cell digital imaging techniques after @entity593 incorporation. RESULTS: @entity9188 inhibited the growth of @entity9058 124 cells in vitro in a dose-dependent manner, given an IC50 value of 1.5 micromol/L. @entity9188 induced apoptosis of FHL124 cells as showed by TUNEL assay. @entity9188 provoked an endoplasmic reticulum (ER) stress response identified through an up regulation of @entity12465 , @entity5593 and @entity14920 (F = 8.51, P = 0.0005). @entity9188 depleted the @entity460 store and consequently lead to a decrease of @entity460 signaling (P = 0.0018). Moreover, @entity9188 had a moderate effect on the @entity460 influx pathway. CONCLUSIONS: @entity9188 inhibits the growth and induces apoptosis of @entity1 lens epithelium cells. @entity9188 provokes an ER stress which could be the cause of apoptic processes.

[Mechanism of @entity4447 induced apoptosis in cultured XXXX lens epithelium cells].

@entity1 :: ('9606', 'Species') :: ['human'] @entity4447 :: ('MESH:C006632', 'Chemical') :: ['arsenic trioxide'] @entity12465 :: ('83939', 'Gene') :: ['EIF2A'] @entity9188 :: ('CHEBI:30621', 'Chemical') :: ['As2O3'] @entity9058 :: ('MESH:D051359', 'Disease') :: ['FHL'] @entity593 :: ('MESH:D016257', 'Chemical') :: ['Fura-2'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity14920 :: ('22926', 'Gene') :: ['ATF6'] @entity5593 :: ('2081', 'Gene') :: ['ERN1']
@entity1 :: (9606,Species) :: ['human']
