UNASSIGNED: Tendon and ligament (T/L) pathologies account for a significant portion of @entity85 and disorders. Tissue engineering has emerged as a promising solution in the regeneration of both tissues. Specifically, the use of multipotent @entity1 mesenchymal stromal cells (hMSC) has shown great promise to serve as both a suitable cell source for tenogenic regeneration and a source of trophic factors to induce tenogenesis. Using four donor sets, we investigated the bidirectional paracrine tenogenic response between @entity1 hamstring tenocytes (hHT) and bone marrow-derived hMSC. Cell metabolic assays showed that only one hHT donor experienced sustained notable increases in cell metabolic activity during co-culture. Histological staining confirmed that co-culture induced elevated collagen protein levels in both cell types at varying time-points in two of four donor sets assessed. Gene expression analysis using qPCR showed the varied up-regulation of anabolic and catabolic markers involved in extracellular matrix maintenance for @entity16591 . Furthermore, analysis of hMSC/hHT co-culture secretome using a reporter cell line for @entity2533 , a potent inducer of tenogenesis, revealed a trend of higher @entity2533 bioactivity in hMSC secretome compared to hHT. Finally, hHT cytoskeletal immunostaining confirmed that both cell types released soluble factors capable of inducing favorable tenogenic morphology, comparable to control levels of soluble @entity2533 . These results suggest a potential for @entity2533 -mediated signaling mechanism that is involved during the paracrine interplay between the two cell types that is reminiscent of T/L matrix remodeling/turnover. These findings have significant implications in the clinical use of hMSC for common T/L pathologies. J. Cell. Biochem. 117: 684-693, 2016.    2015 Wiley Periodicals, Inc.

Cross-Talk Between XXXX Tenocytes and Bone Marrow Stromal Cells Potentiates Extracellular Matrix Remodeling In Vitro.

@entity1 :: ('9606', 'Species') :: ['human'] @entity85 :: ('MESH:D009140', 'Disease') :: ['musculoskeletal injuries'] @entity2533 :: ('7040', 'Gene') :: ['TGF-b', 'TGF-b1'] @entity16591 :: ('9242', 'Gene') :: ['hMSC and hHT']
@entity1 :: (9606,Species) :: ['Human']
