Kluyveromyces marxianus converts whey-borne sugar into @entity1046 , an environmentally friendly solvent with many applications. K. marxianus DSM 5422 presumably synthesizes @entity1046 from acetyl-SCoA. @entity68 limitation as a trigger for this synthesis is explained by a diminished aconitase and @entity4588 dehydrogenase activity (both enzymes depend on @entity68 ) causing diversion of acetyl-SCoA from the tricarboxic acid cycle to @entity13507 synthesis. @entity920 limitation as another trigger for @entity13507 synthesis in this @entity375 refers to involvement of the electron transport chain (all ETC complexes depend on @entity68 and complex IV requires @entity920 ). This hypothesis was checked by using several ETC inhibitors. @entity12930 was ineffective but @entity68545 partially inhibited complex II and initiated @entity13507 synthesis. @entity8581 and @entity3789 as complexes III and IV inhibitors initiated @entity13507 synthesis only at moderate levels while higher concentrations disrupted all respiration and caused @entity1068 formation. A restricted supply of @entity26 (the terminal electron acceptor) also initiated some @entity13507 synthesis but primarily forced @entity1068 production. A switch from aerobic to anaerobic conditions nearly stopped @entity13507 synthesis and induced @entity1068 formation. Iron-limited ester formation was compared with anaerobic @entity1068 production; the @entity13507 yield was lower than the @entity1068 yield but a higher market price, a reduced number of process stages, a faster process, and decreased expenses for product recovery by stripping favor biotechnological @entity13507 production.

Studies on the mechanism of synthesis of XXXX in Kluyveromyces marxianus DSM 5422.

@entity1046 :: ('MESH:C007650', 'Chemical') :: ['ethyl acetate'] @entity4588 :: ('MESH:D019802', 'Chemical') :: ['succinate'] @entity8581 :: ('MESH:D000968', 'Chemical') :: ['Antimycin A'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity12930 :: ('MESH:C030290', 'Chemical') :: ['Malonate'] @entity3789 :: ('CHEBI:17514', 'Chemical') :: ['cyanide'] @entity920 :: ('MESH:D003300', 'Chemical') :: ['copper', 'Copper'] @entity375 :: ('4932', 'Species') :: ['yeast'] @entity13507 :: ('CHEBI:35701', 'Chemical') :: ['ester'] @entity1068 :: ('MESH:D000431', 'Chemical') :: ['ethanol'] @entity68545 :: ('MESH:D002261', 'Chemical') :: ['carboxin'] @entity68 :: ('MESH:D007501', 'Chemical') :: ['iron', 'Iron']
@entity1046 :: (MESH:C007650,Chemical) :: ['ethyl acetate']
