@entity460 and @entity460 -dependent processes have been hypothesized to be involved in the induction of @entity302 . It has been shown that @entity302 neurons have altered @entity460 homeostatic mechanisms following epileptogenesis in the hippocampal neuronal culture (HNC) and @entity2134 models of @entity302 . To investigate the mechanisms causing these alterations in [ @entity141 ]i homeostatic processes following epileptogenesis, we utilized the HNC model of in vitro ' @entity302 ' which produces spontaneous recurrent @entity302 (SREDs). Using [ @entity141 ]i imaging, studies were initiated to evaluate the mechanisms mediating these changes in [ @entity141 ]i homeostasis. ' @entity302 ' neurons required much longer to restore a @entity2227 induced [ @entity141 ]i load to baseline levels than control neurons. Inhibition of @entity141 entry through voltage and receptor gated @entity141 channels and stretch activated @entity141 channels had no effect on the prolonged @entity2227 induced increase in [ @entity141 ]i in @entity302 neurons. Employing @entity2426 , an inhibitor of the sarco/endoplasmic reticulum @entity460 ATPase (SERCA), it was shown that @entity2426 inhibited sequestration of [ @entity141 ]i by SERCA was significantly decreased in ' @entity302 ' neurons. Using @entity141 induced @entity141 release (CICR) cell permeable inhibitors for the @entity8452 receptor (dantrolene) and the @entity9197 ( @entity15258 , @entity15258 ) mediated CICR, we demonstrated that CICR was significantly augmented in the ' @entity302 ' neurons, and determined that the @entity9197 mediated CICR was the major release mechanism altered in epileptogenesis. These data indicate that both inhibition of SERCA and augmentation of CICR activity contribute to the alterations accounting for the impaired @entity460 homeostatic processes observed in ' @entity302 ' neurons. The results suggest that persistent changes in [ @entity141 ]i levels following epileptogenesis may contribute to the long-term plasticity changes manifested in @entity302 and that understanding the basic mechanisms mediating these changes may provide an insight into the development of novel therapeutic approaches to treat @entity302 and prevent or reverse epileptogenesis.

Epileptogenesis induces long-term alterations in intracellular @entity460 release and sequestration mechanisms in the hippocampal neuronal culture model of XXXX .

@entity2227 :: ('CHEBI:29987', 'Chemical') :: ['glutamate'] @entity2426 :: ('MESH:D019284', 'Chemical') :: ['thapsigargin'] @entity8452 :: ('MESH:D012433', 'Chemical') :: ['ryanodine'] @entity15258 :: ('MESH:C109986', 'Chemical') :: ['2APB', '2-amino-ethoxydiphenylborate'] @entity2134 :: ('MESH:D010862', 'Chemical') :: ['pilocarpine'] @entity9197 :: ('3710', 'Gene') :: ['IP3 receptor'] @entity302 :: ('MESH:D004827', 'Disease') :: ['epileptic', 'Epileptic', 'epilepsy', 'epileptiform discharges'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium', 'Calcium'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+']
@entity302 :: (MESH:D004827,Disease) :: ['epilepsy']
