OBJECTIVE: To investigate the anti- @entity5 effects of crude extract from Melia toosendan Sieb. et Zucc and its possible molecular mechanisms in vitro and in vivo. METHODS: Transonic alcohol- @entity3636 extraction method was used to extract toosendanin from the bark of Melia toosendan Sieb. et Zucc, and the content of @entity51324 in the crude extract was measured by high performance liquid chromatography (HPLC). Anti- @entity5 effects of crude extract from Melia toosendan Sieb. et Zucc were investigated in in vivo and in vitro studies. In the in vitro experiment, @entity1 @entity157 cell lines SMMC-7721 and Hep3B were co-incubated with toosendanin crude extract of different concentrations, respectively. In the in vivo experiment, BALB/c @entity19 were subcutaneously inoculated with @entity19 @entity157 H22 cells and treated with crude extract. RESULTS: HPLC revealed the content of toosendanin was about 15%. Crude extract from Melia toosendan Sieb. et Zucc inhibited @entity5 cells growth in a dose- and time-dependent manner. The 50% inhibitory concentration (IC50, 72 h) was 0.6 mg/L for SMMC-7721 cells and 0.8 mg/L for Hep3B cells. Both high-dose [0.69 mg/(kg d)] and low-dose [0.138 mg/(kg d)] crude extract could markedly suppress @entity5 growth, and the inhibition rate was greater than 50%. @entity1710 and @entity1711 staining showed @entity39 area in @entity5 and transmission electron microscopy displayed @entity39 and apoptotic @entity5 cells with apoptotic bodies. Immunohistochemistry showed that the expression of @entity589 and Fas increased and the expression of @entity588 reduced. CONCLUSIONS: Toosendanin extract has potent anti- @entity5 effects via suppressing proliferation and inducing apoptosis of @entity5 cells in vivo and in vitro. The mechanism of apoptosis involves in mitochondrial pathway and death receptor pathway.

Anticancer effects of crude extract from Melia toosendan Sieb. et XXXX in vitro and in vivo.

@entity1 :: ('9606', 'Species') :: ['human'] @entity589 :: ('581', 'Gene') :: ['Bax'] @entity588 :: ('596', 'Gene') :: ['Bcl-2'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrotic'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancers', 'cancer'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity3636 :: ('MESH:D002725', 'Chemical') :: ['chloroform'] @entity51324 :: ('MESH:C036454', 'Chemical') :: ['toosendanin'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['Hematoxylin'] @entity157 :: ('MESH:D006528', 'Disease') :: ['hepatocellular carcinoma']
@entity157 :: (MESH:D006528,Disease) :: ['Zucc on hepatocellular carcinoma']
