Blow flies (Diptera: Calliphoridae) are typically the first insects to arrive at @entity1 remains and @entity12358 . Predictable succession patterns and known larval development of necrophagous insects on vertebrate remains can assist a forensic entomologist with estimates of a minimum post-mortem interval (PMImin) range. However, adult blow flies are infrequently used to estimate the PMImin, but rather are used for a confirmation of larval species identification. Cuticular @entity9436 have demonstrated potential for estimating adult blow @entity238 age, as @entity9436 are present throughout blow @entity238 development, from egg to adult, and are stable structures. The goal of this study was to identify @entity7294 profiles associated with the adults of a North American native blow @entity238 species, Cochliomyia macellaria (Fabricius) and a North American invasive species, Chrysomya rufifacies (Macquart). Flies were reared at a constant temperature (25  C), a photoperiod of 14:10 (L:D) (h), and were provided water, sugar and powdered milk ad libitum. Ten adult females from each species were collected at day 1, 5, 10, 20, and 30 post-emergence. @entity7294 compounds were extracted and then identified using gas chromatography-mass spectrometry (GC-MS) analysis. A total of 37 and 35 compounds were detected from C. macellaria and Ch. rufifacies, respectively. There were 24 and 23 n- @entity22568 and @entity5498 -branched alkane hydrocarbons from C. macellaria and Ch. rufifacies, respectively (10 compounds were shared between species), used for statistical analysis. Non-metric multidimensional scaling analysis and permutational multivariate analysis of variance were used to analyze the @entity7294 profiles with significant differences (P<0.001) detected among post-emergence age cohorts for each species, and unique @entity7294 profiles detected as each adult blow @entity238 species aged. This work provides empirical data that serve as a foundation for future research into improving PMImin estimates made by forensic practitioners and potentially increase the use of adult insects during death investigations.

XXXX profiles throughout adult Calliphoridae aging: A promising tool for forensic entomology.

@entity1 :: ('9606', 'Species') :: ['human'] @entity12358 :: ('MESH:D001474', 'Disease') :: ['carrion'] @entity22568 :: ('CHEBI:32878', 'Chemical') :: ['alkene'] @entity7294 :: ('CHEBI:24632', 'Chemical') :: ['hydrocarbon', 'Hydrocarbon'] @entity9436 :: ('MESH:D006838', 'Chemical') :: ['hydrocarbons'] @entity5498 :: ('CHEBI:32875', 'Chemical') :: ['methyl'] @entity238 :: ('7227', 'Species') :: ['fly']
@entity7294 :: (CHEBI:24632,Chemical) :: ['Hydrocarbon']
