Astrogliosis is a nearly ubiquitous response to a variety of insults to the central nervous system (CNS). This reaction is triggered rapidly, but can persist for years after the initial @entity130 . Little is known about the signaling mechanisms responsible for this activation and its chronic maintenance. Extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) activation is implicated in several functions important to the reactive glial phenotype such as cellular proliferation and motility. Here we utilize immunohistochemistry with a phosphorylation state-specific antibody ( @entity2986 ) to characterize the temporal and spatial pattern of ERK/MAPK activation in neurons and glia following a @entity75 ( @entity75 ) in @entity19 . Early activation (1 h) was primarily in perilesional neuronal elements, particularly of the hippocampus. Occasional perilesional glia were also positive for @entity2986 . Additionally, ependymal cells bilaterally stained prominently for @entity2986 . These patterns of @entity2986 immunoreactivity at 1 h were abolished by pretreatment with the selective @entity13438 inhibitor, @entity37257 . ERK/MAPK activation at later time points between 1 day (d) and 30 d was primarily restricted to perilesional astrocytes with maximum labeling at 3 d. However, @entity2986 -positive astrocytes represented only a subset of total GFAP-positive cells and were found more proximal to the lesion suggesting specific functional activation of these cells. Finally, immunostaining for the phosphorylated form of @entity268 response element-binding ( @entity15983 ) protein, a downstream target of the ERK/MAPK cascade, was increased in perilesional glia 7 d after @entity75 . Sustained activation of the ERK/MAPK signaling pathway in perilesional reactive glia suggests a critical role for this cascade in astrogliosis.

Transient neuronal but persistent astroglial activation of ERK/MAP kinase after focal brain injury in XXXX .

@entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity2986 :: ('13666(Tax:10090)', 'Gene') :: ['pERK'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity75 :: ('MESH:D001927', 'Disease') :: ['FSL', 'forebrain stab lesion'] @entity13438 :: ('17242(Tax:10090)', 'Gene') :: ['MEK'] @entity15983 :: ('12912(Tax:10090)', 'Gene') :: ['CREB'] @entity37257 :: ('MESH:C424127', 'Chemical') :: ['SL327'] @entity130 :: ('MESH:D014947', 'Disease') :: ['trauma']
@entity19 :: (10090,Species) :: ['mice']
