The skin of @entity1 with an extensive @entity130 is repaired by a process that leaves a @entity2805 without sweat glands and therefore loses the function of perspiration. The aim of this study was to identify whether the key factors related to sweat gland development could directly reprogram fibroblasts into sweat gland-like cells. After introducing the NF-kB and @entity7763 genes into fibroblasts, we found that stably transfected fibroblasts expressed specific markers of sweat glands, including @entity787 , @entity5822 , @entity6881 and @entity4166 , both at the protein and mRNA levels. The immunofluorescence staining also showed positive expression of @entity787 , @entity5822 , @entity6881 and @entity4166 in induced fibroblasts, but there were no positive cells in the control groups. The expression of @entity4851 and @entity4910 , downstream genes of NF-kB and @entity7763 , were also significantly increased during regeneration. The induced fibroblasts were implanted into an animal model. Twenty days later, @entity1496 -starch perspiration tests showed that 7 out of the 10 cell-treated paws were positive for perspiration, with a distinctive black point-like area appearing in the center of the paw. Contralateral paws tested negative. Histological examination of skin biopsies from experimental and control paws revealed that sweat glands were fully reconstructed in the test paws, with integral, secretory and ductal portions, but were not present in the control paws. This is the first report of successful reprogramming of fibroblasts into sweat gland-like cells, which will provide a new cell source for sweat gland regeneration in @entity1 with extensive deep burns.

Direct reprogramming of XXXX fibroblasts into sweat gland-like cells.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity7763 :: ('51176', 'Gene') :: ['Lef-1'] @entity1496 :: ('MESH:D007455', 'Chemical') :: ['iodine'] @entity4166 :: ('3880', 'Gene') :: ['CK19'] @entity4910 :: ('595', 'Gene') :: ['Cyclin D1'] @entity787 :: ('1084', 'Gene') :: ['CEA'] @entity5822 :: ('3855', 'Gene') :: ['CK7'] @entity4851 :: ('6469', 'Gene') :: ['Shh'] @entity2805 :: ('MESH:D017439', 'Disease') :: ['hypertrophic scar'] @entity6881 :: ('3861', 'Gene') :: ['CK14'] @entity130 :: ('MESH:D014947', 'Disease') :: ['deep burn injury']
@entity1 :: (9606,Species) :: ['human']
