UNASSIGNED: The basis for acute @entity410 following @entity131 ( @entity131 ) remains unclear. Animal models of @entity131 have revealed @entity528 that could underlie @entity410 activity, but studying initiating events causing hyperexcitability is difficult in these models. In vitro models of @entity130 with cultured cortical neurons, a surrogate for @entity131 , allow facile investigation of cellular changes after injury but they have only demonstrated @entity130 . The goal of this study was to determine if neuronal hyperexcitability could be triggered by in vitro @entity130 . Controlled @entity130 was delivered to a spatially delimited region of a spontaneously active network of cultured @entity35 cortical neurons, yielding a region of stretch-injured neurons and adjacent regions of non-stretched neurons that did not directly experience @entity130 . Spontaneous electrical activity was measured in non-stretched and stretch-injured neurons, and in control neuronal networks not subjected to @entity130 . Non-stretched neurons in stretch-injured cultures displayed a three-fold increase in action potential firing rate and bursting activity 30-60min post-injury. Stretch-injured neurons, however, displayed dramatically lower rates of action potential firing and bursting. These results demonstrate that acute hyperexcitability can be observed in non-stretched neurons located in regions adjacent to the site of @entity130 , consistent with reports that @entity410 activity can arise from regions surrounding the site of localized @entity1545 . Thus, this in vitro procedure for localized @entity528 may provide a model to study the earliest cellular changes in neuronal function associated with acute post-traumatic @entity410 .

Effect of XXXX on action potential and network activity of @entity35 neocortical neurons in culture.

@entity1545 :: ('MESH:D020214', 'Disease') :: ['brain injury'] @entity410 :: ('MESH:D012640', 'Disease') :: ['seizure', 'seizures'] @entity131 :: ('MESH:D001930', 'Disease') :: ['TBI', 'traumatic brain injury'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity528 :: ('MESH:D009410', 'Disease') :: ['acute hyperexcitablility in cortical neurons', 'neuronal stretch injury'] @entity130 :: ('MESH:D014947', 'Disease') :: ['post-injury hypoexcitability', 'stretch injury', 'uniaxial stretch injury']
@entity131 :: (MESH:D001930,Disease) :: ['acute stretch injury']
