The derivatization of biogenic @entity4478 such as @entity7224 , @entity7226 , @entity11667 and @entity8037 with @entity42817 in solid phase extraction cartridges is described. Different types of filling materials were tested in order to have the highest retention of the different analytes. The best results were obtained by using C18 cartridges. The optimal conditions were: @entity6115 solution buffered at pH 12, 2 mM @entity42817 ( @entity1324 - @entity3157 solution 20 mM (pH 9-9.5), 2 + 3 v/v) as reagent concentration, room temperature and 30 min reaction time. The developed procedure was applied to the determination of these @entity4663 in urine samples from healthy controls and @entity5 @entity1 using HPLC with @entity69882 as internal standard. The concentrations ranged from 0.5 to 5 micrograms @entity19264 and the detection limits were 10 ng @entity19264 for all @entity4663 . By concentrating the urine extracts, the detection limits were improved down to 2 ng @entity19264 . The accuracy and the precision of the method were tested. The proposed dansylation method is advantageous with respect to solution dansylation. It improves the total analysis time, avoids high temperatures that can affect the thermal stability of the derivatives and could make possible the automation of the procedure.

Urine @entity4663 determination using XXXX derivatization in solid-phase extraction cartridges and HPLC.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity1324 :: ('MESH:D000096', 'Chemical') :: ['acetone'] @entity8037 :: ('MESH:D013096', 'Chemical') :: ['spermine'] @entity6115 :: ('CHEBI:32952', 'Chemical') :: ['amine'] @entity7226 :: ('MESH:D002103', 'Chemical') :: ['cadaverine'] @entity7224 :: ('MESH:D011700', 'Chemical') :: ['putrescine'] @entity4478 :: ('MESH:D000588', 'Chemical') :: ['amines'] @entity3157 :: ('CHEBI:17544', 'Chemical') :: ['bicarbonate'] @entity4663 :: ('MESH:D011073', 'Chemical') :: ['polyamines'] @entity69882 :: ('MESH:C015127', 'Chemical') :: ['1,7-diaminoheptane'] @entity19264 :: ('16728(Tax:10090)', 'Gene') :: ['mL-1'] @entity42817 :: ('MESH:C029038', 'Chemical') :: ['dansyl chloride'] @entity11667 :: ('MESH:D013095', 'Chemical') :: ['spermidine']
@entity42817 :: (MESH:C029038,Chemical) :: ['dansyl chloride']
