Lipases are the enzymes of choice for laundry detergent industries, owing to their @entity412 removing ability from the soiled fabric, which eventually reduces the usage of @entity4613 -based chemical cleansers in the detergent formulation. In this study, a novel thermo-alkaline lipase-producing strain identified as @entity8220 was isolated from the soil samples of olive oil mill. Enhanced lipase production was observed at 55 degrees C, pH 11 and after 48 h of incubation. Among the substrates tested, @entity6122 (a @entity505 source), peptone (a @entity1568 source) and olive oil at a concentration of 1% were suitable substrates for enhancing lipase production. @entity2400 and @entity9266 were suitable substrates for maximizing lipase production. The enzyme was purified to homogeneity by a single CM-Sephadex column chromatography and revealed molecular mass of 67 kDa. The enzyme (BL1) was active over a wide range of pH from 9.0 to 13.0, with an optimum at pH 11.0, exhibited maximal activity at 55 degreesC and retained more than 70% of its activity after incubation at 70 degrees C or pH 13 for 0.5 h or 24 h, respectively. The enzyme hydrolyzed both short and long-chain @entity1674 at comparable rates. BL1 was studied in a preliminary evaluation for use in detergent formulation solutions. This novel lipase showed extreme stability towards non-ionic and anionic surfactants after pre-incubation for 1 h at 40 degrees C, and good stability towards oxidizing agents. Additionally, the enzyme showed excellent stability and compatibility with various commercial detergents, suggesting its potential as an additive in detergent formulations.

Thermostable, alkaline and detergent-tolerant lipase from a newly isolated thermophilic XXXX .

@entity6122 :: ('MESH:D014994', 'Chemical') :: ['xylose'] @entity1568 :: ('MESH:D009584', 'Chemical') :: ['nitrogen'] @entity8220 :: ('1422', 'Species') :: ['Bacillus stearothermophilus'] @entity1674 :: ('MESH:D014280', 'Chemical') :: ['triacylglycerols'] @entity4613 :: ('D010710', 'Chemical') :: ['phosphate'] @entity2400 :: ('CHEBI:32599', 'Chemical') :: ['MgSO4'] @entity412 :: ('CHEBI:17855', 'Chemical') :: ['triglyceride'] @entity505 :: ('MESH:D002244', 'Chemical') :: ['carbon'] @entity9266 :: ('MESH:D011136', 'Chemical') :: ['Tween-80']
@entity8220 :: (1422,Species) :: ['Bacillus stearothermophilus']
