OBJECTIVE: To observe the genes expression of @entity519 inducible factor la ( @entity7738 ) and @entity2032 by inducing chondrogenic differentiation of @entity1 bone marrow mesenchymal stem cells (hBMSCs) so as to provide a fundamental basis for HIF involving in the mechanism of chondrogenesis. METHODS: High density pellet of hBMSCs was obtained by centrifugation and cultured with @entity8108 -DMEM medium containing 2% fetal bovine serum (control group) and with chondrogenic medium (chondrogenic induction group) under @entity519 (2% O2) for 3 weeks. Immunohistochemistry staining was utilized to identify extracellular proteoglycan and collagen type II at 3 weeks after culture. Western blot was applied for measuring @entity7738 and @entity2032 protein levels at 1 week after culture. Real-time quantitative PCR was performed to detect the genes expressions of @entity7738 , @entity2032 , @entity1935 , collagen type II, collagen type X, and Aggrecan at 1, 2, and 3 weeks after culture. RESULTS: @entity11979 staining showed sparse nucleus in the control group, and dense nucleus in the chondrogenic induction group; extracellular matrix staining was deeper in the chondrogenic induction group than the control group. Immunohistochemical staining for collagen type II was positive in cytoplasm; when compared with the chondrogenic induction group, the control group showed sparse and light-coloured nucleus. At 1 week after culture, the protein expression levels of @entity7738 and @entity2032 in the chondrogenic induction group were significantly lower than those in the control group (t = 8.345, P = 0.001; t = 7.683, P = 0.002). When compared with control group, the @entity7738 mRNA expression was significantly down-regulated at 1 week and significantly up-regulated at 2 weeks in chondrogenic induction group (P < 0.05), but no significant difference was found at 3 weeks between the 2 groups (P > 0.05). And the mRNA expression of @entity2032 was significantly down-regulated and mRNA expression of @entity1935 was significantly up-regulated after chondrogenic differentiation when compared with the control group (P < 0.01). The mRNA expressions of collagen type II and collagen type X were significantly up-regulated at 2 and 3 weeks after chondrogenic differentiation when compared with the control group (P < 0.05). And the mRNA expression of Aggrecan was significantly up-regulated at each time point after chondrogenic differentiation (P < 0.05). CONCLUSION: @entity7738 may involve the hBMSCs chondrogenic differentiation under @entity519 , while @entity2032 expression is depressed throughout the period and may have negative effect on differentiation.

[HYPOXIA INDUCIBLE FACTOR la/2a GENES EXPRESSION IN CHONDROGENIC DIFFERENTIATION OF XXXX BONE MARROW MESENCHYMAL STEM CELLS].

@entity1 :: ('9606', 'Species') :: ['human'] @entity7738 :: ('3091', 'Gene') :: ['HIF-1a'] @entity11979 :: ('MESH:D014048', 'Chemical') :: ['Toluidine blue'] @entity2032 :: ('2034', 'Gene') :: ['HIF-2a', 'HIF- 2a'] @entity519 :: ('MESH:D000860', 'Disease') :: ['hypoxia'] @entity8108 :: ('D006859', 'Chemical') :: ['H'] @entity1935 :: ('6662', 'Gene') :: ['Sox-9']
@entity1 :: (9606,Species) :: ['HUMAN']
