UNASSIGNED: Selective @entity750 reuptake inhibitors are known to have a @entity74 that are often linked to the endocrine system e.g. hormonal imbalances, @entity0 , @entity250 , and @entity1866 . The mechanisms behind most of these disorders are not known in details. In this study we investigated whether the endocrine effect due to SSRI exposure could be detected in well adopted in vitro steroidogenesis assays, two versions of the aromatase enzyme inhibition assay and the H295R cell assay. The five drugs @entity837 , @entity905 , @entity3755 , @entity903 and @entity3756 , were shown to inhibit the aromatase enzyme in both types of aromatase assays. The IC50 values ranged from 3 to 600 M. All five SSRIs, were further investigated in the H295R cell line. All compounds altered the @entity479 secretion from the cells, the lowest observed effect levels were 0.9 M and 3.1 M for @entity3756 and @entity3755 , respectively. In general the H295R cell assay was more sensitive to @entity18060 exposure than the two aromatase assays, up to 20 times more sensitive. This indicates that the H295R cell line is a better tool for screening endocrine disrupting effects. Our findings show that the endocrine effects of SSRIs may, at least in part, be due to interference with the steroidogenesis.

Effects of selective XXXX reuptake inhibitors on three sex steroids in two versions of the aromatase enzyme inhibition assay and in the H295R cell assay.

@entity18060 :: ('D017367', 'Chemical') :: ['SSRI'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast enlargement'] @entity905 :: ('MESH:D005473', 'Chemical') :: ['fluoxetine'] @entity3755 :: ('MESH:D016666', 'Chemical') :: ['fluvoxamine'] @entity903 :: ('MESH:D017374', 'Chemical') :: ['paroxetine'] @entity3756 :: ('MESH:D020280', 'Chemical') :: ['sertraline'] @entity1866 :: ('MESH:D008599', 'Disease') :: ['menstrual cycle disorders'] @entity837 :: ('MESH:D015283', 'Chemical') :: ['citalopram'] @entity74 :: ('MESH:D030342', 'Disease') :: ['range of disorders'] @entity750 :: ('MESH:D012701', 'Chemical') :: ['serotonin'] @entity479 :: ('D013256', 'Chemical') :: ['steroid'] @entity250 :: ('MESH:D012735', 'Disease') :: ['sexual dysfunction']
@entity750 :: (MESH:D012701,Chemical) :: ['serotonin']
