UNASSIGNED: As a possible route for invasion of the central nervous system (CNS), circulating @entity9925 ) in the blood is believed to traverse the blood-brain barrier (BBB), resulting in @entity3328 . However, the underlying mechanism is poorly understood. In this study, we demonstrated that @entity19 @entity69 receptor 1 ( @entity16344 ) is responsible for PV attachment to the cell surface, allowing invasion into the CNS via BBB. PV interacts with the apical domain of @entity16344 on @entity19 brain capillary endothelial cells (MBEC4) in a dose-dependent manner, via its capsid protein (VP1). We found that F-G, G-H, and @entity8108 -I loops in VP1 are important for this binding. However, C-D, D-E, and E-F loops in VP1-fused Venus proteins efficiently penetrate MBEC4 cells. These results imply that VP1 functional domain responsible for cell-attachment is different from that involved in viral permeation of the brain capillary endothelium. We observed that co-treatment of MBEC4 cells with excess PV particles but not dextran resulted in blockage of @entity69 transport into cells. Using the transwell in vitro BBB model, @entity69 co-treatment inhibited permeation of PV into MBEC4 cells and delayed further viral permeation via @entity16344 knockdown. Together, @entity16344 as a positive mediator of PV-host cell attachment and PV permeation of MBEC4 cells, our results indicate a novel role of @entity8282 as a cellular receptor for @entity1 receptor (hPVR)/ @entity27278 -independent PV invasion of the CNS.

@entity69 Receptor 1 Facilitates @entity9925 Permeation of XXXX Brain Capillary Endothelial Cells.

@entity1 :: ('9606', 'Species') :: ['human PV'] @entity16344 :: ('22042(Tax:10090)', 'Gene') :: ['mTfR1'] @entity3328 :: ('MESH:D011051', 'Disease') :: ['paralytic poliomyelitis'] @entity27278 :: ('5817', 'Gene') :: ['CD155'] @entity9925 :: ('138950', 'Species') :: ['poliovirus (PV'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity69 :: ('7018', 'Gene') :: ['transferrin'] @entity8282 :: ('7037', 'Gene') :: ['TfR1'] @entity8108 :: ('D006859', 'Chemical') :: ['H']
@entity19 :: (10090,Species) :: ['Mouse']
