Bone marrow-derived mesenchymal stem cells (MSCs), which have beneficial effects in @entity647 ( @entity647 ), can serve as a vehicle for gene therapy. @entity24287 ( @entity24287 ), a counter-regulatory enzyme of @entity14206 that degrades angiotensin (Ang) II into Ang 1-7, has a protective role against @entity647 . Because @entity24287 expression is severely reduced in the injured lung, a therapy targeted to improve @entity24287 expression in lung might attenuate @entity647 . We hypothesized that MSCs overexpressing @entity24287 would have further benefits in lipopolysaccharide (LPS)-induced @entity647 @entity19 , when compared with MSCs alone. MSCs were transduced with @entity24287 gene (MSC- @entity24287 ) by a lentiviral vector and then infused into wild-type (WT) and @entity24287 knockout ( @entity24287 (-/y)) @entity19 following an LPS-induced @entity651 . The results demonstrated that the @entity651 of @entity647 @entity19 was alleviated at 24 and 72 h after MSC- @entity24287 transplantation. MSC- @entity24287 improved the lung histopathology and had additional anti-inflammatory effects when compared with MSCs alone in both WT and @entity24287 (-/y) @entity647 @entity19 . MSC- @entity24287 administration also reduced pulmonary vascular permeability, improved endothelial barrier integrity, and normalized lung eNOS expression relative to the MSC group. The beneficial effects of MSC- @entity24287 could be attributed to its recruitment into the injured lung and enhanced local expression of @entity24287 protein without changing the serum @entity24287 levels after MSC- @entity24287 transplantation. The biological activity of the increased @entity24287 protein decreased the Ang II amount and increased the Ang 1-7 level in the lung when compared with the @entity647 and MSC-only groups, thereby inhibiting the detrimental effects of accumulating Ang II. Therefore, compared to MSCs alone, the administration of MSCs overexpressing @entity24287 resulted in a further improvement in the inflammatory response and pulmonary endothelial function of LPS-induced @entity647 @entity19 . These additional benefits could be due to the degradation of Ang II that accompanies the targeted overexpression of @entity24287 in the lung.

Mesenchymal Stem Cells Overexpressing Angiotensin-Converting Enzyme 2 Rescue Lipopolysaccharide-Induced XXXX .

@entity19 :: ('10090', 'Species') :: ['mice'] @entity651 :: ('MESH:D055370', 'Disease') :: ['lung injury', 'intratracheal lung injury'] @entity14206 :: ('11421(Tax:10090)', 'Gene') :: ['ACE'] @entity24287 :: ('70008(Tax:10090)', 'Gene') :: ['ACE2', 'Angiotensin converting enzyme 2'] @entity647 :: ('MESH:D055371', 'Disease') :: ['acute lung injury', 'ALI']
@entity651 :: (MESH:D055370,Disease) :: ['Lung Injury']
