OBJECTIVE: To understand developmental characteristics of @entity150 ( @entity150 ) by evaluating genomic alterations and @entity876 protein expression in @entity5 , their recurrences, and in the morphologically normal urothelium of @entity150 @entity1 . METHODS: @entity5 and their respective recurrences, six low-grade and five high-grade cases, provided 19 samples that were submitted to laser microdissection capture followed by high resolution comparative genomic hybridization (HR-CGH). HR-CGH profiles went through two different analyses--all @entity5 combined or classified according to their respective histologic grades. In a supplementary analysis, 124 primary @entity5 , their recurrences, and normal urothelium biopsied during the period between @entity5 surgical resection and recurrence, were submitted to immunohistochemical analyses of the @entity876 protein. During the follow-up of at least 21 @entity1 , urinary bladder washes citologically negative for neoplastic cells were submitted to fluorescence in situ hybridization (FISH) to detect copy number alterations in centromeres 7, 17, and 9p21 region. RESULTS AND CONCLUSIONS: HR-CGH indicated high frequencies (80%) of gains in 11p12 and losses in 16p12, in line with suggestions that these chromosome regions contain genes critical for @entity1494 . Within a same @entity1 , @entity5 and their respective recurrences showed common genomic losses and gains, which implies that the genomic profile acquired by @entity5 was relatively stable. There were exclusive genomic alterations in low and in high grade @entity5 . Genes mapped in these regions should be investigated on their involvement in the @entity1494 . @entity5 from same @entity1 did not present similar levels of protein @entity876 expression; however, when cases were grouped according to @entity5 histologic grades, @entity876 expression was directly proportional to @entity5 grades. Biopsies taken during the follow-up of @entity1 with history of previously resected @entity150 revealed that 5/15 @entity1 with no histologic alterations had more than 25% of urothelial cells expressing the @entity876 protein, suggesting that the apparently normal urothelium was genomically unstable. No numerical alterations of the chromosomes 7, 17, and 9p21 region were found by FISH during the periods "free-of- @entity5 ." Our data are informative for further studies to better understand urinary bladder urothelial carcinogenesis.

Chromosomal imbalances in successive moments of XXXX bladder urothelial carcinoma.

@entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity1494 :: ('MESH:D001745', 'Disease') :: ['urinary bladder carcinogenesis'] @entity5 :: ('MESH:D009369', 'Disease') :: ['neoplasia', 'tumor', 'Tumors', 'Successive tumors', 'primary tumors', 'urothelial tumors', 'tumors'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity150 :: ('MESH:D001749', 'Disease') :: ['urinary bladder carcinomas', 'UBC']
@entity1 :: (9606,Species) :: ['human']
