C3H/He and Balb/c @entity19 bearing SCC VII and EMT6/KU @entity5 , respectively, received continuous administration of @entity1603 ( @entity1603 ) for 5 days using implanted mini-osmotic pumps to label all proliferating (P) cells. @entity4571 was administered intraperitoneally before @entity900 injection and/or @entity5 were locally heated at 40 degrees C for 60 min immediately after @entity900 injection. The @entity5 were then excised, minced and trypsinized. The @entity5 cell suspensions were incubated with cytochalasin-B (a cytokinesis-blocker), and the micronucleus (MN) frequency in cells without @entity1603 labeling (quiescent (Q) cells) was determined using immunofluorescence staining for @entity1603 . The MN frequency in total (P+Q) @entity5 cells was determined from @entity5 that had not been pretreated with @entity1603 labeling. The sensitivity to @entity900 was evaluated in terms of the frequency of induced micronuclei in binuclear @entity5 cells (MN frequency). In both @entity5 systems, the MN frequency in Q cells was lower than that in the total cell population. @entity4571 treatment elevated the MN frequency in total SCC VII cells. Mild heating raised the MN frequency more markedly in Q cells than in total cells. The combination of @entity4571 and mild heat treatment increased the MN frequency more markedly than either treatment alone. In total SCC VII cells, @entity4571 increased 195mPt- @entity900 uptake. Mild heating elevated 195mPt- @entity900 uptake in total @entity19 /KU cells. @entity900 -sensitivity of Q cells was lower than that of total cells in both @entity5 systems. @entity4571 sensitized @entity5 cells including a large acutely @entity519 fraction, such as those of SCC VII @entity5 , through inhibition of the fluctuations in @entity5 blood flow. @entity5 cells including a large chronically @entity519 fraction such as Q cells were thought to be sensitized by mild heating through an increase in @entity5 blood flow.

Augmentation in chemosensitivity of intratumor quiescent cells by combined treatment with XXXX and mild hyperthermia.

@entity1603 :: ('MESH:D001973', 'Chemical') :: ['BrdU', "5-bromo-2'-deoxyuridine"] @entity19 :: ('10090', 'Species') :: ['mice', 'EMT6'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'Tumor', 'tumors'] @entity900 :: ('MESH:D002945', 'Chemical') :: ['cisplatin', 'Cisplatin'] @entity519 :: ('MESH:D000860', 'Disease') :: ['hypoxic'] @entity4571 :: ('MESH:D009536', 'Chemical') :: ['Nicotinamide', 'nicotinamide']
@entity4571 :: (MESH:D009536,Chemical) :: ['nicotinamide']
