OBJECTIVE: @entity1691 ( @entity1691 ) is a highly heritable disease with @entity1530 being the strongest susceptible gene. In order to survey the environmental triggers for @entity625 development, we used a high-throughput technique to screen the effects of 12,264 chemicals on the @entity1530 gene promoter. METHODS: Promoter reporter transfectants 293T- @entity1530 and HeLa- @entity1530 were tested using robotics with 12,264 chemicals. Chemicals that modulated @entity1530 promoter activity > 150% or < 40% were selected for further evaluation of IC50/EC50 and cell viability. RESULTS: The primary screening using the 293T- @entity1530 promoter reporter cell line yielded 5.1% hits that either suppressed (556 chemicals) or enhanced (68 chemicals) the @entity1530 promoter activity. A secondary reconfirmation screening was carried out with these 624 candidates using HeLa- @entity1530 promoter reporter cells under several different culture conditions. The yield of positive candidates was 130, of which 47 were derived from natural products. Based on the bio-information of those positive natural products, 21 chemicals were selected for analysis by dose-response IC50/EC50 experiments. Eight compounds showed potential pharmacological activities. Two suppressors are both derived from an herbal medicine (lei gong teng) that has been used for decades to treat @entity1200 . The 6 activators all belonged to a group of chemicals known as @entity3088 , widely distributed among dietary fruits and vegetables. CONCLUSION: Several common dietary products that contain certain @entity3088 might be environmental risk factors for @entity1691 ; the Chinese traditional herb lei gong teng might be a potential drug for @entity1 who are @entity1530 -positive. These results provide new research directions for the pathogenesis and therapeutics of @entity1691 .

High-throughput screening of chemical libraries for modulators of gene promoter activity of HLA-B2705: environmental pathogenesis and therapeutics of XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity1530 :: ('3106', 'Gene') :: ['HLA-B27'] @entity625 :: ('MESH:D001168', 'Disease') :: ['arthritis'] @entity3088 :: ('MESH:D005419', 'Chemical') :: ['flavonoids'] @entity1691 :: ('MESH:D013167', 'Disease') :: ['Ankylosing spondylitis', 'AS'] @entity1200 :: ('MESH:D007154', 'Disease') :: ['immune diseases']
@entity1691 :: (MESH:D013167,Disease) :: ['ankylosing spondylitis']
