BACKGROUND: Grape @entity566 is considered rare; grape lipid transfer protein (LTP; @entity2739 v 1), an endochitinase and a @entity18228 ( @entity18228 ) have been reported as grape allergens. A considerable number of @entity1 have referred to our department for severe reactions to grapes, and several IgE binding proteins were detected. OBJECTIVES: The aim of this study was to identify and characterise the allergens involved in severe @entity56 to grapes and describe the population in which they occur. METHODS: @entity1 with reported severe @entity56 to grapes (n = 37) are described. Grape allergens were purified/fractionated by a combination of chromatographic techniques, identified by proteomic analysis and biochemically characterised. Immunoreactivity was assessed by blot (inhibitions) and RAST (inhibitions), and skin prick tests were performed with the isolated allergens. RESULTS: All subjects were polyallergic, sensitised and reactive to several additional foods and pollen. All @entity1 were sensitised to grape LTP. A 28-kDa expansin, a 37.5-kDa polygalacturonase-inhibiting protein, a 39-kDa beta-1,3-glucanase and a 60-kDa protein were identified as minor grape allergens. Endochitinase and @entity18228 did not play a role. Inhibition experiments revealed the possible cross-reactive role of LTP for clinical sensitivities to other LTP-containing plant foods, but also the involvement of cross-reactive @entity1735 determinants of minor allergens in IgE cross-reactivity. CONCLUSIONS: LTP is the major grape allergen, while additional minor allergens may contribute to clinical reactivity. Severe grape @entity566 presents in @entity1097 @entity1 who frequently react to other LTP-containing, plant-derived foods. The 'LTP syndrome' is the appropriate term to describe this condition.

Severe immediate XXXX to grapes: part of a lipid transfer protein-associated clinical syndrome.

@entity1 :: ('9606', 'Species') :: ['Patients', 'patients'] @entity566 :: ('MESH:D006967', 'Disease') :: ['allergy'] @entity1097 :: ('MESH:C566404', 'Disease') :: ['atopic'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['carbohydrate'] @entity2739 :: ('5212', 'Gene') :: ['Vit'] @entity18228 :: ('9519', 'Gene') :: ['thaumatin-like protein', 'TLP'] @entity56 :: ('MESH:D004342', 'Disease') :: ['allergic reactions']
@entity56 :: (MESH:D004342,Disease) :: ['allergic reactions']
