AIM: To experimentally assess protective effect of Immunovac-VP-4 vaccine against @entity778 . MATERIALS AND METHODS. Immunization of @entity19 with polycomponent vaccine Immunovac-VP-4 was performed using oral or mucosal route of administration (intranasally, orally, and with combined nasal-oral method). Immunized @entity19 were inoculated intranasally by influenza virus H5N2 adapted for @entity19 . Survival of @entity19 in experimental and control (intact) groups was assessed daily during 14 days. Survival and death rates of @entity19 were determined. Levels of cytokines in sera of @entity19 from both groups were measured by enzyme immunoassay. RESULTS: Half of experimental animals survived after triple subcutaneous administration of vaccine in dose 20 mcg and subsequent intranasal challenge with @entity778 . Single subcutaneous immunization with dose 400 mcg resulted in survival of 80 +/- 12.6% of @entity19 after challenge. Triple intranasal and combined intranasal-oral immunization as well as after triple subcutaneous immunization resulted in survival of half of challenged @entity19 . In control group challenge was lethal for 90 - 100% of @entity19 . Same methods of immunization lead to increase of @entity777 , IL-12, @entity21692 , and @entity60 levels. CONCLUSION: Data about significant protective effect after immunization with Immunovac-VP-4 against @entity778 were obtained. Immunovac-VP-4 administered by mentioned routes activated nasal-associated lymphoid tissue providing first line defense at entry site of @entity281 , which demonstrates need to further study of this vaccine during development of strategy for non-specific prophylaxis of @entity281 .

[Protective activity of Immunovac-VP-4 vaccine against XXXX administered by different methods].

@entity778 :: ('MESH:D015658', 'Disease') :: ['avian influenza virus H5N2'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity60 :: ('15978(Tax:10090)', 'Gene') :: ['IFN-gamma'] @entity21692 :: ('16168(Tax:10090)', 'Gene') :: ['IL-15'] @entity777 :: ('16193(Tax:10090)', 'Gene') :: ['IL-6'] @entity281 :: ('MESH:D007239', 'Disease') :: ['influenza infection']
@entity778 :: (MESH:D015658,Disease) :: ['avian influenza virus H5N2']
