BACKGROUND: Increased permeability and uptake of proinflammatory bacterial endotoxins from gut microbiota has been suggested as a mechanism for @entity28 that causes @entity28 -associated insulin resistance. We hypothesized that intestinal barrier function may be restored after Roux-en-Y gastric bypass (RYGB) surgery and thereby contribute to decreased @entity32 . The objective of this study was to investigate levels of the permeability-regulating tight-junction proteins in @entity1 small intestinal mucosa before and after RYGB surgery. METHODS: Paired intraindividual jejunal mucosa samples were retrieved at the time of surgery and 6 to 8 months after surgery. Mucosal cell surface area was calculated by histomorphometry. Mucosal samples were analyzed by proteomics to find patterns of protein regulations. Based on these findings further analyses were performed by Western blotting. Ussing chambers were used to analyze permeability in the retrieved mucosal samples. RESULTS: Mucosal surface area was significantly decreased after surgery. Global protein expression analysis showed a significant increase in the @entity10585 ( @entity10585 ), which was confirmed by Western blotting. Further analyses showed a significant increase in @entity21487 expression after surgery, whereas @entity11177 and zonula occludens-1 levels were decreased. Expressions of @entity3670 and vinculin were unchanged. Ussing chamber experiments revealed a linear correlation between the epithelial electrical resistance and @entity14187 protein expression. CONCLUSION: Several alterations were found in the rerouted small intestine after surgery, indicating a decreased jejunal mucosal surface area and decreased paracellular permeability. These changes could contribute to decreased uptake of luminal microbiota-derived inflammatory mediators such as endotoxins after RYGB.

Expression of tight-junction proteins in XXXX proximal small intestinal mucosa before and after Roux-en-Y gastric bypass surgery.

@entity1 :: ('9606', 'Species') :: ['human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity21487 :: ('1365;1364', 'Gene') :: ['claudin-3 and -4'] @entity28 :: ('MESH:D009765', 'Disease') :: ['obesity-associated chronic inflammation', 'obesity'] @entity10585 :: ('3856', 'Gene') :: ['Ck8', 'cytokeratin-8'] @entity11177 :: ('100506658', 'Gene') :: ['occludin'] @entity3670 :: ('9076', 'Gene') :: ['claudin-1, -2, -5'] @entity14187 :: ('1365', 'Gene') :: ['claudin-3']
@entity1 :: (9606,Species) :: ['human']
