PURPOSE: In a series of @entity14 ( @entity14 ) @entity1 , we evaluated the sensitivities of the individual microsatellites recommended by the National @entity5 Institute (NCI) consensus workshop for detection of @entity2198 ( @entity124 ). On the basis of this evaluation, we developed a three-marker assay that assigns @entity124 ( @entity124 ) in a multiplex polymerase chain reaction. METHODS: Individual marker sensitivity was assessed in 18 @entity14 . Multiplex and NCI assays were then assessed in a series of 120 @entity1 with early-onset @entity694 . RESULTS: The sensitivity of microsatellite markers BAT25, BAT26, D2S123, D5S346, and D17S250 for ASI in @entity14 was 100%, 94%, 72%, 50%, and 50%, respectively. The three most accurate markers were combined and optimized in a multiplex assay that assigned @entity124 whenever at least two of three markers revealed ASI. In early-onset @entity694 , the prevalence of @entity124 determined by the multiplex assay and by the NCI assay was 16% and 23%, respectively. The @entity5 and @entity1 with @entity124 identified by the NCI assay lacked the traits characteristic of @entity124 seen in @entity5 and @entity1 identified by the multiplex assay: retention of heterozygosity (NCI additional 22% v multiplex 84%; P =.003), characteristic @entity5 morphology (0% v 64%; P =.006), and 5-year @entity5 survival rate (44% v 100%; P =.0003). CONCLUSION: The multiplex assay identifies @entity694 with @entity124 by assessing three highly accurate microsatellite markers. This assay identifies a smaller @entity124 cohort with more homogeneous clinical features and is superior as a marker of favorable prognosis. It merits prospective evaluation as a marker of prognosis and as a screening test for @entity14 .

Automated, multiplex assay for @entity2198 in XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity2198 :: ('MESH:D006316', 'Disease') :: ['high-frequency microsatellite instability'] @entity124 :: ('MESH:D053842', 'Disease') :: ['MSI-H', 'MSI', 'microsatellite instability'] @entity5 :: ('MESH:D009369', 'Disease') :: ['additional MSI-H tumors', 'tumor', 'cancer', 'Cancer', 'tumors'] @entity14 :: ('MESH:D015179', 'Disease') :: ['hereditary nonpolyposis colorectal cancer', 'HNPCC', 'HNPCC tumors', 'HNPCC cancers'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon cancer', 'colon cancers']
@entity14 :: (MESH:D015179,Disease) :: ['colorectal cancer']
