Reactivity of the @entity10811 -binding Helix pomatia agglutinin (HPA) in @entity5 has been associated with poor prognosis and @entity3 development. In our @entity16911 /FEMX-I @entity1 @entity320 model, the binding of @entity15069 correlates with experimental lung @entity3 formation in athymic @entity19 . In the present study, the metastatic potential of 2 @entity1 @entity320 cell lines ( @entity16911 and FEMX-I) was assessed in relation to @entity1735 and invasive phenotype. Immunocytological and invasion assays highlighted significant differences between these 2 cell lines. Immuno-cytochemical analysis confirmed the widespread expression of HPA-binding glycoconjugates on @entity16911 but not FEMX-I cells. One of these HPA-binding glycoconjugates, the Tn antigen, was expressed highly on the surface of @entity16911 cells but only weakly in the cytoplasm of FEMX-I cells. The sialyl Tn antigen was expressed in FEMX-I but not in @entity16911 cells. There was no difference between the cell lines in adhesion/rate of trapping in athymic nude @entity19 lung tissues. In Matrigel invasion assays, @entity16911 cells demonstrated an invasion potential more than 6 times greater than that observed with FEMX-I cells. Matrigel invasion of @entity16911 cells was inhibited after incubation with HPA (89%) compared to controls with HPA and @entity26243 blocking sugar or without HPA (p < 0.0005 at 5 df). In contrast, there was no inhibitory effect with the anti-Tn antibody @entity19 . Invasion of FEMX-I cells was not affected by the lectin and the IE3 antibody. Immuno-cytochemical analysis revealed expression of the terminal @entity3649 - and @entity25656 -binding lectin galectin 3 ( @entity14813 ) in these @entity320 cell lines. Expression of both the lectin and its receptor may be a contributory feature in the pulmonary invasion of @entity16911 @entity320 cells. Overall, our findings suggest that HPA-binding glycoconjugates other than the @entity70164 -O-Ser/Thr of the Tn antigen may be important in the extracellular matrix invasion of @entity16911 @entity320 cells.

Invasion potential and @entity10811 expression in a XXXX @entity320 model.

@entity1 :: ('9606', 'Species') :: ['human'] @entity14813 :: ('3958', 'Gene') :: ['Mac-2'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumours'] @entity16911 :: ('4015', 'Gene') :: ['LOX'] @entity15069 :: ('CHEBI:61431', 'Chemical') :: ['HPA'] @entity19 :: ('10090', 'Species') :: ['mouse', 'IE3', 'nude mice'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['carbohydrate'] @entity70164 :: ('CHEBI:40356', 'Chemical') :: ['alphaGalNAc'] @entity3649 :: ('MESH:D005690', 'Chemical') :: ['galactose'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity26243 :: ('CHEBI:28037', 'Chemical') :: ['GalNAc'] @entity25656 :: ('MESH:C066929', 'Chemical') :: ['polylactosamine'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma'] @entity10811 :: ('CHEBI:28800', 'Chemical') :: ['N-acetylgalactosamine']
@entity1 :: (9606,Species) :: ['human']
