INTRODUCTION: The saliva of blood-feeding animals (e.g., mosquitoes, ticks, bats) has pharmacological activities that facilitate efficient blood-sucking. We previously identified a unique anti-platelet protein, anopheline anti-platelet protein (AAPP), from the salivary gland of female Anopheles stephensi ( @entity1 @entity311 vector mosquito). AAPP specifically blocks platelet adhesion to collagen by binding directly to collagen and subsequently aggregating platelets. To examine the potential of AAPP as a therapeutic agent, we investigated the in vivo anti- @entity715 effects of AAPP. MATERIALS AND METHODS: Effects of AAPP on whole blood/platelet aggregation in @entity19 were examined. AAPP was also challenged in an established model of @entity1151 in @entity19 . We simultaneously investigated the side-effects of the protein (prolongation of bleeding time and coagulation time). @entity117 was used as a positive control for comparison of anti- @entity715 effects. RESULTS AND CONCLUSIONS: AAPP inhibited whole blood aggregation induced by collagen at 10mg/kg body weight. AAPP prevented @entity204 at a lower dose (3mg/kg) without prolongation of bleeding time compared with @entity117 (100mg/kg) that compromised hemostasis. AAPP and @entity117 did not affect coagulation time. These results indicate that AAPP has great potential as a new anti-platelet agent with a better risk/benefit ratio than that seen with @entity117 (the most widely used anti-platelet agent).

Anopheline anti-platelet protein from a @entity311 vector mosquito has anti- XXXX effects in vivo without compromising hemostasis.

@entity1 :: ('9606', 'Species') :: ['human'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity117 :: ('MESH:D001241', 'Chemical') :: ['Aspirin', 'aspirin'] @entity1151 :: ('MESH:D011655', 'Disease') :: ['pulmonary thromboembolism'] @entity311 :: ('MESH:D008288', 'Disease') :: ['malaria'] @entity204 :: ('MESH:D003643', 'Disease') :: ['pulmonary death'] @entity715 :: ('MESH:D013927', 'Disease') :: ['thrombotic']
@entity715 :: (MESH:D013927,Disease) :: ['thrombotic']
