AIM: To study expression of topoisomerases (TI) I and II alpha (DNA-bound enzymes involved in transcription and replication) in renal tissue as markers of activity and prognosis of @entity631 ( @entity631 ) decisive for choice of immunodepressive therapy. MATERIAL AND METHODS: TI expression was studied immunohistochemically in renal biopsies from 177 @entity1 with different morphological variants of @entity631 and in the samples of unaffected kidney tissue removed in 12 @entity1 for @entity5 . RESULTS: There are definite differences between proliferative and non-proliferative @entity631 variants--elevation of TI levels and monocytic infiltration in proliferative @entity631 . Focal-segmental @entity631 is characterized by a high TI II alpha level in mesangial cells and monocytic infiltration of the glomeruli which are typical for @entity32 . A statistical relationship between TI levels in mesangial cells and glomerular epithelium suggests a pathogenetic relation between these links of the pathological process. Molecular markers of activation and proliferation of cells and direct inductors of the inflammatory process (cells of monocytic infiltrate) closely correlated with the activity index--an integral indicator of inflammatory activity, as well as with the integral indicator of @entity3808 processes in renal tissue-- @entity1463 index. Monocytic infiltration in the interstitium correlated both with morphological manifestations of activity, progression of @entity1310 and their clinical equivalents. In high TI expression @entity631 resistance to immunodepressive therapy rose. To overcome the resistance, immunodepressive therapy must be more active--large doses and duration of treatment. In @entity1 with @entity1988 and mesangiocapillary @entity631 renal prognosis was worse in the presence of high TI expression in mesangial cells and epithelium of the renal canaliculi. CONCLUSION: The authors are the first to demonstrate TI expression in renal tissue of @entity631 @entity1 , correlation of its level with activity of renal process as well as its role in prediction of response to treatment and the rate of @entity177 . It is suggested that high TI expression entails a progressive course of @entity631 .

[Clinical implications of DNA-topoisomerases examination in renal biopsies from @entity1 with XXXX ].

@entity1 :: ('9606', 'Species') :: ['patients'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity1310 :: ('MESH:D009393', 'Disease') :: ['nephritis'] @entity5 :: ('MESH:D009369', 'Disease') :: ['local tumors'] @entity3808 :: ('MESH:C538213', 'Disease') :: ['sclerotic'] @entity1988 :: ('MESH:D008181', 'Disease') :: ['lupus nephritis'] @entity1463 :: ('MESH:D012598', 'Disease') :: ['sclerosis'] @entity177 :: ('MESH:D051437', 'Disease') :: ['renal failure progression'] @entity631 :: ('MESH:D005921', 'Disease') :: ['GN', 'glomerulosclerosis', 'glomerulonephritis']
@entity1310 :: (MESH:D009393,Disease) :: ['nephritis']
