@entity4990 ( @entity4991 ) is a biolipid that has diverse biological activities implicated in @entity43 initiation and progression. Previous studies have shown the critical role of the Rho/Rho-associated kinase (ROCK) pathway in @entity4991 -induced @entity43 progression. However, detailed underlying mechanism by which the Rho/ROCK pathway induces @entity43 cell invasion is still incompletely understood. In the present study, we observed that the Rho/ROCK pathway is implicated in the production of proteolytic enzymes, leading to @entity4991 -induced @entity43 cell invasion. @entity4991 induced @entity1495 expression in @entity1 and PA-1 cells and @entity2290 ( @entity2290 ) expression in SKOV-3 cells. @entity4991 -induced proteolytic enzyme expression was required for the invasion of @entity43 cells expressing corresponding enzymes. Pretreatment of cells with a pharmacological inhibitor of Rho/ROCK (Y-27632) or overexpression of a dominant-negative mutant of Rho (Rho N19) profoundly inhibited @entity4991 -induced proteolytic enzyme expression as well as the invasive potential of @entity43 cells. In addition, transfection with dominant-negative Ras (Ras N17) significantly inhibited @entity4991 -induced Rho activation as well as @entity1495 and @entity2290 expression. Consistently, @entity12223 reduced @entity4991 -induced nuclear factor (NF)-kB activation that is critical for proteolytic enzyme expression and cellular invasion. Collectively, we demonstrate a mechanism by which @entity4991 promotes @entity43 progression through coordinate activation of a Ras/Rho/ROCK/NF-kB signaling pathway and the proteolytic enzyme secretion, providing novel biomarkers and promising therapeutic targets for @entity43 cell progression.

The Rho/ROCK pathway for XXXX -induced proteolytic enzyme expression and @entity43 cell invasion.

@entity43 :: ('MESH:D010051', 'Disease') :: ['ovarian cancer'] @entity1 :: ('9606', 'Species') :: ['CAOV-3'] @entity4990 :: ('MESH:C032881', 'Chemical') :: ['Lysophosphatidic acid'] @entity4991 :: ('CHEBI:52288', 'Chemical') :: ['LPA'] @entity12223 :: ('MESH:C108830', 'Chemical') :: ['Y-27632'] @entity1495 :: ('4318', 'Gene') :: ['MMP-9', 'matrix metalloproteinase (MMP)-9'] @entity2290 :: ('5328', 'Gene') :: ['urokinase-type plasminogen activator', 'uPA']
@entity4990 :: (MESH:C032881,Chemical) :: ['lysophosphatidic acid']
