Intact actin microfilaments are required for insulin-regulated @entity413 transporter isoform 4 ( @entity2368 ) translocation to the plasma membrane. Lipoxygenase (LO) metabolites have recently been shown to contribute to the regulation of actin cytoskeleton rearrangement. In the present investigation, ventricular cardiomyocytes were used to study the effects of two structurally different LO inhibitors ( @entity3642 and @entity2604 ) on insulin signalling events, @entity413 uptake, @entity2368 translocation and the actin network organization. Insulin stimulation increased @entity413 uptake 3-fold in control cells, whereas LO inhibition completely blocked this effect. This was paralleled by a slight reduction in the insulin-induced @entity669 phosphorylation of insulin receptor substrate (IRS)-1 and @entity21320 . However, inhibition of 12-LO did not affect the association of @entity4357 3-kinase with @entity10175 and the phosphorylation of @entity584 / @entity25449 in response to insulin. Addition of @entity11903 almost completely restored the insulin action in cells exposed to @entity2604 . Insulin stimulation increased cell surface @entity2368 2-fold in control cells, whereas LO inhibition abrogated the insulin-stimulated @entity2368 translocation. LO inhibition induced a prominent disassembly of actin fibres compared with control cells. In conclusion, we show here that @entity11903 plays a role in the organization of the actin network in cardiomyocytes. LO inhibition blocks @entity2368 translocation without affecting downstream insulin signalling. These data suggest that LO metabolites participate in the regulation of @entity413 transport by contributing to a rearrangement of actin cytoskeletal elements.

Eicosanoids participate in the regulation of cardiac XXXX transport by contribution to a rearrangement of actin cytoskeletal elements.

@entity10175 :: ('3667', 'Gene') :: ['IRS-1'] @entity4357 :: ('MESH:D010716', 'Chemical') :: ['phosphatidylinositol'] @entity3642 :: ('MESH:C007628', 'Chemical') :: ['esculetin'] @entity584 :: ('207', 'Gene') :: ['Akt'] @entity2604 :: ('MESH:D009637', 'Chemical') :: ['nordihydroguaiaretic acid'] @entity25449 :: ('2185', 'Gene') :: ['protein kinase B'] @entity11903 :: ('MESH:D019377', 'Chemical') :: ['12(S)-hydroxyeicosatetraenoic acid'] @entity2368 :: ('6517', 'Gene') :: ['GLUT4'] @entity21320 :: ('8660', 'Gene') :: ['IRS-2'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity413 :: (MESH:D005947,Chemical) :: ['glucose']
