UNASSIGNED: In this work we investigated how modifications of the @entity141 homeostasis in anterior lens epithelial cells (LECs) are associated with different types of @entity231 (cortical or nuclear) and how the progression of the @entity231 (mild or moderate) affects the @entity141 signaling. We systematically analyzed different aspects of intra- and inter-cellular @entity141 signaling in the @entity1 LECs, which are attached to surgically isolated lens capsule (LC), obtained during @entity231 surgery. We monitored the temporal and spatial changes in intracellular @entity141 concentration after stimulation with @entity934 by means of @entity593 fluorescence captured with an inverted microscope. In our analysis we compared the features of @entity141 signals in individual cells, synchronized activations, spatio-temporal grouping and the nature of intercellular communication between LECs. The latter was assessed by using the methodologies of the complex network theory. Our results point out that at the level of individual cells there are no significant differences when comparing the features of the signals with regard either to the type or the stage of the @entity231 . On the other hand, noticeable differences are observed at the multicellular level, despite inter-capsule variability. LCs associated with more developed @entity231 were found to exhibit a slower collective response to stimulation, a less pronounced spatio-temporal clustering of LECs with similar signaling characteristics. The reconstructed intercellular networks were found to be sparser and more segregated than in LCs associated with mild @entity231 . Moreover, we show that spontaneously active LECs often operate in localized groups with quite well aligned @entity141 activity. The presence of spontaneous activity was also found to affect the stimulated @entity141 responses of individual cells. Our findings indicate that the @entity231 progression entails the impairment of intercellular signaling thereby suggesting the functional importance of altered @entity141 signaling of LECs in cataractogenesis.

The Analysis of Intracellular and Intercellular Calcium Signaling in XXXX Anterior Lens Capsule Epithelial Cells with Regard to Different Types and Stages of the @entity231 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity231 :: ('MESH:D002386', 'Disease') :: ['cataracts', 'cataract'] @entity593 :: ('MESH:D016257', 'Chemical') :: ['Fura-2'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+'] @entity934 :: ('MESH:D000109', 'Chemical') :: ['acetylcholine']
@entity1 :: (9606,Species) :: ['Human']
