UNASSIGNED: The presence of @entity5 stem cells (CSCs) has major implications in the choice of @entity5 treatment strategy and is responsible for @entity5 relapse. CSCs have been isolated and characterized in several types of @entity5 ; however, studies concerning the CSCs from @entity189 ( @entity189 ) are limited. Thus, the present study was designed to isolate and characterize the @entity5 stem-like side population (SP) cells from @entity189 samples. The fluorescence-activated cell sorting (FACS)-based @entity5956 dye exclusion technique identified that 3.9% of cells from @entity189 samples were @entity5 stem-like SP cells. Upon treatment with @entity1855 (ABC transporter inhibitor), the percentage of SP cells was significantly reduced to 0.7%, which confirms that the ABC transporter protein exhibits a significant role in drug exclusion. Fluorescence microscopy analysis revealed that the FACS purified SP cells showed increased expression of @entity10430 ( @entity855 transporter protein), @entity4729 and @entity1759 (stem cell surface protein). Furthermore, these SP cells exhibited increased mRNA expression of @entity10430 and anti-apoptotic factor @entity3752 , which contribute to multi-drug resistance and increased cell survival rate. Notably, the Wnt/b-catenin signaling pathways are altered in SP cells. In addition, using reverse transcription  -quantitative polymerase chain reaction analysis it was observed that the cells exhibited increased expression of @entity5741 and @entity18609 . In conclusion, data from the present study clearly demonstrated that the presence of @entity5 stem-like SP cells from @entity189 may be responsible for chemotherapeutic drug resistance, @entity5 recurrence and invasion.

Abnormal Wnt signaling and overexpression of XXXX contributes to drug efflux properties of side population cells in @entity189 .

@entity5956 :: ('MESH:C017807', 'Chemical') :: ['Hoechst 33342'] @entity4729 :: ('5460', 'Gene') :: ['Oct-4'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity1855 :: ('MESH:D014700', 'Chemical') :: ['verapamil'] @entity10430 :: ('9429', 'Gene') :: ['ABCG2'] @entity3752 :: ('648', 'Gene') :: ['Bmi-1'] @entity18609 :: ('8313', 'Gene') :: ['AXIN2'] @entity189 :: ('MESH:D002277', 'Disease') :: ['nasopharyngeal carcinoma', 'NPC'] @entity1759 :: ('960', 'Gene') :: ['CD44'] @entity5741 :: ('22943', 'Gene') :: ['DKK1'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity10430 :: (9429,Gene) :: ['ABCG2']
