It has been suggested that store-operated @entity141 entry (SOC) facilitates @entity2922 secretion and synthesis in @entity291 adrenal medullary (AM) cells. However, there has been no experimental result clearly showing that cation channel activity is enhanced by store @entity141 depletion. Thus the present experiments were undertaken to address the issue of whether @entity35 AM cells have SOC channels. Inhibition of the sarco(endo)plasmic reticulum @entity141 (SERCA) pump resulted in a sustained increase in intracellular @entity141 concentration ([ @entity141 ](i)) in @entity35 AM cells. This increase was completely suppressed by 2 mM @entity3411 but not by 100 muM D600. A bath application of @entity3411 , but not D600, produced an outward current at -60 mV in @entity35 AM cells, whereas exposure to a SERCA pump inhibitor did not affect either the whole cell current level or the @entity3411 -induced outward current. The refilling of intracellular store sites was suppressed by the addition of @entity3411 to the perfusate. RT-PCR revealed that transcripts for @entity32781 ( @entity32781 ) and 5 ( @entity66246 ) were present in @entity35 adrenal medullas. Immunocytochemistry showed that @entity32781 channels, which have been implicated in SOC in certain types of cells, were mainly localized in the endoplasmic reticulum (ER) and not in the plasma membrane, and that @entity31525 , a @entity141 sensor in the ER, was not expressed in @entity35 AM cells. On the basis of these results, we conclude that @entity35 AM cells lack the SOC mechanism.

Ca2+ pathway involved in the refilling of store sites in XXXX adrenal medullary cells.

@entity35 :: ('10116', 'Species') :: ['rat'] @entity31525 :: ('361618(Tax:10116)', 'Gene') :: ['STIM1'] @entity66246 :: ('140933(Tax:10116)', 'Gene') :: ['TRPC5'] @entity3411 :: ('CHEBI:49786', 'Chemical') :: ['Ni(2+)'] @entity2922 :: ('CHEBI:33567', 'Chemical') :: ['catecholamine'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity32781 :: ('89821(Tax:10116)', 'Gene') :: ['transient receptor potential channels 1', 'TRPC1'] @entity291 :: ('9913', 'Species') :: ['bovine']
@entity35 :: (10116,Species) :: ['rat']
