@entity1583 cells are attractive targets for gene therapy. However, no satisfactory vectors are currently available. A major problem with the most commonly used adenovirus vectors, based on @entity9504 ( @entity9504 ) or @entity11650 , is their low binding efficiency for @entity1583 cells. In this study we identify two adenovirus serotypes with high affinity for @entity1583 cells. The binding efficiency of prototype serotypes @entity3667 , @entity17615 , and Ad35p for different committed @entity1583 cell lines representing T cells (Jurkat), B cells (DG75), monocytes (U937-2), myeloblasts (K562), and granulocytes (HL-60) was evaluated and compared to that of Ad5v, the commonly used adenovirus vector, using flow cytometry. In contrast to Ad5v, which bound to less than 10% of the cells in all experiments, @entity17615 and Ad35p showed high binding efficiency for all of the different @entity1583 cell lines. @entity3667 bound to the lymphocytic cell lines to some extent but less well to the myelomonocytic cell lines. The abilities of the different serotypes to infect, replicate, and form complete infectious particles in the @entity1583 cell lines were also investigated by immunostaining, @entity12486 labeling of viral proteins, and titrations of cell lysates. @entity17615 and Ad35p infected the highest proportion of cells, and @entity17615 infected all of the cell lines investigated. The @entity17615 hexon was expressed equally well in K562 and A549 cells. Jurkat cells also showed high levels of expression of @entity17615 hexons, but the production of infectious particles was low. The binding properties of virions were correlated to their ability to infect and be expressed.

Adenovirus types 11p and 35p show high binding efficiencies for committed XXXX cell lines and are infective to these cell lines.

@entity9504 :: ('MESH:D000257', 'Disease') :: ['adenovirus type 2', 'Ad2'] @entity11650 :: ('8081', 'Gene') :: ['Ad5'] @entity3667 :: ('5664', 'Gene') :: ['Ad4p'] @entity17615 :: ('100188795', 'Gene') :: ['Ad11p'] @entity1583 :: ('MESH:D019337', 'Disease') :: ['hematopoietic', 'Hematopoietic'] @entity12486 :: ('CHEBI:37983', 'Chemical') :: ['(35)S']
@entity1583 :: (MESH:D019337,Disease) :: ['hematopoietic']
