UNASSIGNED: Prions are protein-based infectious entities associated with fatal @entity75 in animals, but also modify a range of host-cell phenotypes in the budding @entity375 , @entity375 . Many questions remain about the evolution and biology of prions. Although several functionally distinct prion-forming proteins exist in @entity375 , [HET-s] of @entity25667 is the only other known fungal prion. Here we investigated prion-like, protein-based epigenetic transmission in the @entity6739 @entity6739 . We show that @entity6739 cells can support the formation and maintenance of the prion form of the @entity375 @entity16578 translation factor [PSI(+)], and that the formation and propagation of these @entity16578 aggregates is inhibited by @entity2097 , indicating commonalities in prion propagation machineries in these evolutionary diverged @entity375 . A proteome-wide screen identified the Ctr4 @entity920 transporter subunit as a putative prion with a predicted prion-like domain. Overexpression of the ctr4 gene resulted in large Ctr4 protein aggregates that were both detergent and proteinase-K resistant. Cells carrying such [CTR(+)] aggregates showed increased sensitivity to oxidative stress, and this phenotype could be transmitted to aggregate-free [ctr(-)] cells by transformation with [CTR(+)] cell extracts. Moreover, this [CTR(+)] phenotype was inherited in a non-Mendelian manner following mating with na  ve [ctr(-)] cells, but intriguingly the [CTR(+)] phenotype was not eliminated by @entity2097 - @entity5209 treatment. Thus, Ctr4 exhibits multiple features diagnostic of other fungal prions and is the first example of a prion in @entity6739 . These findings suggest that transmissible protein-based determinants of traits may be more widespread among fungi.

The XXXX transport-associated protein Ctr4 can form prion-like epigenetic determinants in @entity6739 .

@entity6739 :: ('4896', 'Species') :: ['Schizosaccharomyces pombe', 'S. pombe', 'fission yeast'] @entity75 :: ('MESH:D001927', 'Disease') :: ['brain diseases'] @entity2097 :: ('MESH:D019791', 'Chemical') :: ['guanidine', 'guanidine hydrochloride'] @entity16578 :: ('851752(Tax:4932)', 'Gene') :: ['Sup35'] @entity25667 :: ('5145', 'Species') :: ['Podospora anserina'] @entity920 :: ('MESH:D003300', 'Chemical') :: ['copper'] @entity375 :: ('4932', 'Species') :: ['yeast', 'Saccharomyces cerevisiae', 'yeasts', 'S. cerevisiae'] @entity5209 :: ('CHEBI:36807', 'Chemical') :: ['hydrochloride']
@entity920 :: (MESH:D003300,Chemical) :: ['copper']
