UNASSIGNED: Immunity to infections with @entity805 ) can involve vigorous human leukocyte antigen (HLA) class I-restricted @entity405 (+) cytotoxic T cell (CTL) responses. MV, albeit regarded monotypic, is known to undergo molecular evolution across its RNA genome. To address which regions of the MV proteome are eligible for recognition by @entity405 (+) CTLs and how different HLA class I loci contribute to the epitope display, we interrogated the naturally processed and presented MV peptidome extracted from cell lines expressing in total a broad panel of 16 different common @entity17374 molecules. The repertoire and abundance of MV peptides were bona fide identified by nanoHPLC-MS/MS. -Eighty-nine MV peptides were discovered and assignment to an @entity1529 , -B, or -C allele, based on HLA-peptide affinity prediction, was in most cases successful. Length variation and presentation by multiple HLA class I molecules was common in the MV peptidome. More than twice as many unique MV epitopes were found to be restricted by @entity1530 than by @entity1529 , while MV peptides with supra-abundant expression rates (>5,000   cc) were rather associated with @entity1529 and @entity18698 . In total, 59 regions across the whole MV proteome were identified as targeted by HLA class I. Sequence coverage by epitopes was highest for internal proteins transcribed from the MV-P/V/C and -M genes and for hemagglutinin. At the genome level, the majority of the HLA class I-selected MV epitopes represented codons having a higher non-synonymous mutation rate than silent mutation rate, as established by comparison of a set of 58 unique full length MV genomes. Interestingly, more molecular variation was seen for the epitopes expressed at rates >= 1,000 cc. These data for the first time indicate that HLA class I broadly samples the MV proteome and that CTL pressure may contribute to the genomic evolution of MV.

XXXX Epitope Presentation by HLA: Novel Insights into Epitope Selection, Dominance, and Microvariation.

@entity1530 :: ('3106', 'Gene') :: ['HLA-B'] @entity1529 :: ('3105', 'Gene') :: ['HLA-A'] @entity17374 :: ('3105;3106;3107', 'Gene') :: ['HLA-A, -B, and -C'] @entity805 :: ('11234', 'Species') :: ['measles virus (MV'] @entity18698 :: ('3107', 'Gene') :: ['HLA-C'] @entity405 :: ('925', 'Gene') :: ['CD8']
@entity805 :: (11234,Species) :: ['Measles Virus']
