Withania somnifera is one of the most valuable medicinal plants synthesizing secondary metabolites known as @entity17933 . Despite pharmaceutical importance, limited information is available about the biosynthesis of @entity17933 . Chemo-profiling of leaf and root tissues of Withania suggest differences in the content and/or nature of @entity17933 in different chemotypes. To identify genes involved in chemotype and/or tissue-specific @entity12980 biosynthesis, we established transcriptomes of leaf and root tissues of distinct chemotypes. Genes encoding enzymes for intermediate steps of @entity25164 backbone biosynthesis with their alternatively spliced forms and paralogous have been identified. Analysis suggests differential expression of large number genes among leaf and root tissues of different chemotypes. Study also identified differentially expressing transcripts encoding cytochrome P450s, glycosyltransferases, methyltransferases and transcription factors which might be involved in chemodiversity in Withania. @entity778 induced gene silencing of the @entity5068    7-reductase (WsDWF5) involved in the synthesis of @entity62003 , @entity12980 backbone, suggests role of this enzyme in biosynthesis of @entity17933 . Information generated, in this study, provides a rich resource for functional analysis of @entity12980 -specific genes to elucidate chemotype- as well as tissue-specific @entity12980 biosynthesis. This genomic resource will also help in development of new tools for functional genomics and breeding in Withania.

Comparative transcriptome analysis of different chemotypes elucidates XXXX biosynthesis pathway from medicinal plant Withania somnifera.

@entity778 :: ('MESH:D015658', 'Disease') :: ['Virus'] @entity17933 :: ('MESH:D054358', 'Chemical') :: ['withanolides'] @entity62003 :: ('MESH:C002537', 'Chemical') :: ['24-methylene cholesterol'] @entity12980 :: ('CHEBI:74716', 'Chemical') :: ['withanolide'] @entity25164 :: ('CHEBI:26873', 'Chemical') :: ['terpenoid'] @entity5068 :: ('CHEBI:15889', 'Chemical') :: ['sterol']
@entity12980 :: (CHEBI:74716,Chemical) :: ['withanolide']
