One major difficulty in interpreting the changes occurring during @entity409 is the co-existence of non-activated cells and proliferating hepatocytes at all stages of differentiation. The aim of this study was to investigate @entity6741 (BA) secretion into bile during normal ( @entity70178 ) and synchronized (SLR) @entity409 in @entity35 . Regeneration was synchronized by reversible inhibition of @entity2151 reductase by 10 h treatment with @entity1862 (HU) shortly after two-third partial hepatectomy. Total BA output as measured by GC-MS increased immediately after partial hepatectomy. This was followed by a further transient enhancement during the next day in the @entity70178 . HU treatment did not significantly modify total BA output, but after releasing synchronized regeneration a marked reduction was observed. This was followed by a recovery to reach values close to those of @entity70178 on day 7 of the regenerative process in SLR. Amidated BA output as measured by HPLC analysis revealed an early enhancement in the proportion of non-conjugated BAs in bile in @entity70178 . However, the profile of conjugated BAs, which was not affected by HU treatment, matched that of total BAs during the first stage of SLR. By contrast, the increase in BA output observed on day 3 of the regenerative process in this group was accounted for by an enhancement in non-conjugated BA secretion. On day 7 of the regenerative process, the proportion of conjugated BA in bile was restored to approximately 100% in this group. Most BA molecules were conjugated with @entity2226 rather than with @entity2228 in all experimental groups, during both @entity70178 and SLR. GC-MS determinations indicated that the magnitude of the @entity6742 predominance in all bile samples was significantly modified during @entity409 . This was increased early after partial hepatectomy and declined toward control values after few (2-3) days. Enhancement in the @entity6742 predominance was due to a reduction in the proportion of all other major BAs, above all @entity70179 and @entity17659 . By contrast, minor BAs in normal control @entity35 bile such as @entity70180 were increased during both @entity70178 and SLR, and remained at detectable levels up to day 7. Changes in the proportion of secreted BA species were similar in SLR and @entity70178 except that the early reduction in the proportion of BAs other than @entity6742 was more pronounced in SLR and the quantitative importance of the diversity in BA species was recovered earlier in SLR than in @entity70178 . In summary, these results indicate that profound changes in BA secretion during @entity35 @entity409 do occur. Most of them are probably related to the existence of retro-differentiation/re-differentiation processes which are speeded up by @entity1862 -induced synchronization of the wave of hepatocyte proliferation.

@entity6741 secretion during synchronized XXXX @entity409 .

@entity2226 :: ('MESH:D013654', 'Chemical') :: ['taurine'] @entity70178 :: ('29363(Tax:10116)', 'Gene') :: ['NLR'] @entity2228 :: ('CHEBI:57305', 'Chemical') :: ['glycine'] @entity1862 :: ('MESH:D006918', 'Chemical') :: ['hydroxyurea'] @entity409 :: ('MESH:D008107', 'Disease') :: ['liver regeneration'] @entity6742 :: ('MESH:D019826', 'Chemical') :: ['cholic acid'] @entity17659 :: ('MESH:C004821', 'Chemical') :: ['omega-muricholic acid'] @entity6741 :: ('CHEBI:3098', 'Chemical') :: ['bile acid'] @entity70179 :: ('MESH:C030712', 'Chemical') :: ['ursocholic acid'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity2151 :: ('CHEBI:26561', 'Chemical') :: ['ribonucleotide'] @entity70180 :: ('MESH:C002001', 'Chemical') :: ['allo-cholic acid']
@entity35 :: (10116,Species) :: ['rat']
