UNASSIGNED: The eukaryotic translation initiation factor (eIF) 4G is required during protein synthesis to promote the assembly of several factors involved in the recruitment of a 40S ribosomal subunit to an mRNA. Although many eukaryotes express two @entity69712 isoforms that are highly similar, the @entity69712 isoforms in plants, referred to as @entity69712 and eIFiso4G, are highly divergent in size, sequence, and domain organization but both can interact with eIF4A, @entity69713 , @entity69714 isoforms, and the poly(A)-binding protein. Nevertheless, @entity69712 and eIFiso4G from @entity3505 exhibit preferences in the mRNAs they translate optimally. For example, mRNA containing the 5'-leader (called   ) of @entity21104 preferentially uses @entity69712 in @entity3505 germ lysate. In this study, the @entity69712 isoform specificity of    was used to examine functional differences of the @entity69712 isoforms in @entity958 . As in @entity3505 ,   -mediated translation was reduced in an @entity69712 null mutant. Loss of the @entity69715 isoform, which is similar in sequence to @entity3505 eIFiso4G, did not substantially affect   -mediated translation. However, loss of the @entity69716 isoform substantially reduced   -mediated translation. @entity69716 is substantially divergent from @entity69715 and is present only in the Brassicaceae, suggesting a recent evolution. @entity69716 isoforms exhibit sequence-specific differences in regions representing partner protein and RNA binding sites. Loss of any @entity69712 isoform also resulted in a substantial reduction in reporter transcript level. These results suggest that @entity69716 appeared late in plant evolution and exhibits more functional similarity with @entity69712 than with @entity69715 during   -mediated translation.

Eukaryotic Initiation Factor @entity69715 and XXXX Are Isoforms Exhibiting Distinct Functional Differences in Supporting Translation in @entity958 .

@entity69716 :: ('816939(Tax:3702)', 'Gene') :: ['eIFiso4G2'] @entity21104 :: ('12242', 'Species') :: ['tobacco mosaic virus'] @entity69713 :: ('543417(Tax:4565)', 'Gene') :: ['eIF4B'] @entity69714 :: ('543485(Tax:4565)', 'Gene') :: ['eIF4E'] @entity69712 :: ('825194(Tax:3702)', 'Gene') :: ['eif4g', 'eIF4G'] @entity958 :: ('3702', 'Species') :: ['Arabidopsis'] @entity3505 :: ('4565', 'Species') :: ['wheat'] @entity69715 :: ('835897(Tax:3702)', 'Gene') :: ['eIFiso4G1']
@entity69716 :: (816939(Tax:3702),Gene) :: ['eIFiso4G2']
