Abstract Amorphous @entity3440 nanoparticles ( @entity3758 -NPs) have been studied for their toxic and genotoxic potential. Although contradictory data have been reported and the possible modes of action are not fully elucidated, aneugenic events have been reported, indicating the microtubule (MT) network as a potential target. To investigate this, we examined the effects of 59   nm (10     g/ml) and 174   nm (7.5     g/ml) @entity3758 -NPs on MTs in mitotic and interphase A549 @entity1 @entity2 cells. No gross morphological changes of the mitotic spindle or induction of multipolar spindles were observed upon @entity3758 -NPs treatment. The influence of @entity3758 -NPs on the interphase MTs network dynamics was investigated by in situ depolymerisation/repolymerisation experiments. Results showed a clear increase in MT dynamics after @entity3758 -NP treatment. Consistent with this, reduced levels of MT acetylation were observed. In addition, live cell microscopy demonstrated that @entity3758 -NP treatment reduced A549 cell motility. The @entity3758 -NP doses and conditions (serum-free) used in this study did not induce significant cell @entity137 or MN frequencies. Therefore, the effects on MT dynamics, MT acetylation and migration observed, are direct effects of the @entity3758 -NPs and not a consequence of NP overload or toxic or genotoxic effects.

Amorphous XXXX nanoparticles alter microtubule dynamics and cell migration.

@entity1 :: ('9606', 'Species') :: ['human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung carcinoma'] @entity3758 :: ('CHEBI:30563', 'Chemical') :: ['SiO2'] @entity3440 :: ('MESH:D012822', 'Chemical') :: ['silica']
@entity3440 :: (MESH:D012822,Chemical) :: ['silica']
