After bilateral infusions of @entity3812 or vehicle in the @entity35 nucleus basalis magnocellularis, the time course of changes in several cholinergic and neurotrophin-related markers were assessed. Animals were sacrificed at 3, 7, 14, 28, 35 and 84 days post-lesion, and both the NBM and cortical areas were assessed. Sections were stained immunohistochemically for @entity1877 acetyltransferase ( @entity4268 ) or p140trk (trk) or histochemically for acetylcholinesterase (AChE). @entity4268 activity and neurotrophin protein levels were assessed regionally. The number of @entity4268 immunoreactive NBM neuronal profiles decreased beginning 3 days post-lesion and reach maximal loss by 28 days post-lesion, with no recovery. Examination of trk-IR around the NBM revealed a time-dependent decrease in trk-IR of magnocellular neuron and an increase in trk-IR of astrocytes at 14 and 28 days post-lesion. The density of AChE-stained cortical fibers was maximally decreased 3 days post-lesion followed by an increase in fiber staining across the remaining time points. Cortical @entity4268 activity showed the largest decrease at 7 days followed by recovery 84 days after @entity3812 infusion. There was an increase in NGF in the parietal cortex after @entity3812 infusion but no change in BDNF level. These patterns of changes in the cholinergic and neurotrophin-related markers suggest an association between NGF and lesion-induced compensatory responses in the basal forebrain cholinergic system.

Time course of changes in cholinergic and neurotrophin-related markers after infusion of XXXX into the basal forebrain.

@entity35 :: ('10116', 'Species') :: ['rat'] @entity4268 :: ('8063', 'Species') :: ['ChAT'] @entity1877 :: ('MESH:D002794', 'Chemical') :: ['choline'] @entity3812 :: ('MESH:D003078', 'Chemical') :: ['colchicine']
@entity3812 :: (MESH:D003078,Chemical) :: ['colchicine']
