BACKGROUND/AIMS: For laboratory diagnostics in @entity409 , many enzymes have been used for the assessment of hepatocellular function. Among them, two transaminases, @entity493 and @entity492 aminotransferase, have been regarded as the most sensitive indicators of @entity16 . However, the enhanced enzyme activities of the enzymes do not exactly indicate or represent the cause and progression of diseases in the @entity1 with @entity409 . To overcome such limitations, immunological methods have been suggested as one of the alternatives for the replacement or supplement of the conventional enzymatic analysis. METHODS: In the hope of developing a new assay system for measuring the @entity6571 concentration rather than its activity, we have developed a new assay using fluorescence labeled anti- @entity6571 monoclonal antibodies. Blood was obtained from a normal population of 234 @entity1 and 43 @entity409 @entity1 . The linearity, limit of detection, and performance of the new assay system were tested and evaluated. The comparability of assay was examined with an ELISA and biochemical assays. RESULTS: The linearity fell in the range of 0-1 mg/L of @entity6571 (R=0.995), and the analytical detection limit was 12 microg/L of @entity6571 . The mean recovery of the control was 102.4 % in a working range. The precision of the intra- and inter-assay in a range of 50-800 microg/L was CVs < 7% and CVs < 6%, respectively. In the normal population, the mean @entity6571 concentration was 35.5 microg/L. The mean @entity6571 concentration in @entity1 with @entity409 was 266.5 microg/L. The new assay system correlated well with an ELISA and biochemical assay for quantification of @entity6571 concentration (R=0.92 and 0.88, respectively; N=43). CONCLUSIONS: We have developed a new immunological assay using generated monoclonal antibodies to @entity1 cytosolic @entity6571 and used them for the development of a fluorescent assay measuring the enzyme mass. Cytosolic @entity6571 mass in sera could be measured reproducibly by the immunological method. In conclusion, this study has provided us with a new type of tool for an accurate measurement of the enzyme amount in circulation.

[Development of a method for the immunological measurement of XXXX aminotransferase with monoclonal antibodies].

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity6571 :: ('26503', 'Gene') :: ['AST'] @entity16 :: ('MESH:D009422', 'Disease') :: ['hepatocellular damage'] @entity409 :: ('MESH:D008107', 'Disease') :: ['liver diseases', 'liver disease'] @entity492 :: ('CHEBI:29995', 'Chemical') :: ['aspartate'] @entity493 :: ('CHEBI:16449', 'Chemical') :: ['alanine']
@entity492 :: (CHEBI:29995,Chemical) :: ['aspartate']
