UNASSIGNED: Snake venom protein from @entity24682 (DA), one of the major venomous species in Taiwan, causes @entity6243 leading to @entity204 . Although horse-derived antivenin is a major treatment, relatively high and detrimental side effects are seen occasionally. In our study, yolk immunoglobulin (IgY) was purified from eggs, and DA protein was recognized using Western blotting and an enzyme-linked immunosorbent assay (ELISA), similar to therapeutic horse antivenin. The ELISA also indicated that specific IgY antibodies were elicited after the fifth booster, plateaued, and lasted for at least three months. To generate monoclonal single-chain variable fragment (scFv) antibodies, we used phage display technology to construct two libraries with short or long linkers, containing 6.24 * 10(8) and 5.28 * 10(8) transformants, respectively. After four rounds of bio-panning, the eluted phage titer increased, and the phage-based ELISA indicated that the specific clones were enriched. @entity4334 sequences of 30 individual clones expressing scFv were analyzed and classified into four groups that all specifically recognized the DA venom protein. Furthermore, based on mass spectrometry, the scFv-bound protein was deduced to be snake venom metalloproteinase proteins. Most importantly, both IgY and mixed scFv inhibited the lethal effect in @entity19 injected with the minimum lethal dosage of the DA protein. We suggest that, together, these antibodies could be applied to the development of diagnostic agents or treatments for snakebite envenomation in the future.

Antibodies against Venom of the Snake XXXX .

@entity6243 :: ('MESH:D006474', 'Disease') :: ['hemorrhagic symptoms'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity24682 :: ('36307', 'Species') :: ['Deinagkistrodon acutus'] @entity4334 :: ('D009711', 'Chemical') :: ['Nucleotide'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death']
@entity24682 :: (36307,Species) :: ['Deinagkistrodon acutus']
