UNASSIGNED: The present study was designed to assess the possible protective effects of @entity369 ( @entity534 ) against lipopolysaccharide (LPS) induced inflammatory response in juvenile Jian @entity7136 ( @entity7136 . Jian) in  vivo and in enterocytes in  vitro. Firstly, inflammatory response was established by exposing enterocytes to different concentrations of LPS for 24  h. Secondly, the protective effects of @entity534 against subsequent LPS exposure were studied in enterocytes. Finally, we investigated whether dietary @entity534 supplementation could attenuate immune challenge induced by LPS in  vivo. The result indicated that 10  mg/L LPS could induced inflammatory response in enterocytes. Cells exposed to LPS (10-30  mg/L) alone for 24  h resulted in a significant increase in @entity880 dehydrogenase release (LDH) (P  <  0.05). The cell viability, protein content, alkaline phosphatase activity were decreased by LPS (P  <  0.05). Moreover, LPS exposure significantly increased TNF-a, IL-1b, and IL-6 mRNA expression in  vitro (P  <  0.05). However, pre-treatment with @entity534 remarkably prevented the increase of TNF-a, IL-1b, and IL-6 by inhibiting the excessive activation of TLR4-Myd88 signaling pathway through down-regulating TLR4, Myd88, NFkB p65, and MAPK p38 mRNA expression (P < 0.05). Interestingly, the experiment in  vivo showed that @entity534 pre-supplementation could attenuate immune challenge induced by LPS via TLR4-Myd88 signaling pathway, and thus protect fish against LPS-induced inflammatory response. In conclusion, all of these results indicated pre-supplementation with @entity534 decreased LPS induced @entity1200 and regulated TLR4-Myd88 signaling pathway in juvenile Jian @entity7136 in  vivo and in enterocytes in  vitro.

In  vitro and in  vivo protective effect of @entity369 against lipopolysaccharide induced inflammatory response in the intestine of juvenile Jian XXXX ( @entity7136 var. Jian).

@entity534 :: ('CHEBI:16467', 'Chemical') :: ['Arg'] @entity1200 :: ('MESH:D007154', 'Disease') :: ['immune damage'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity7136 :: ('7962', 'Species') :: ['carp', 'Cyprinus carpio var'] @entity369 :: ('CHEBI:29016', 'Chemical') :: ['arginine']
@entity7136 :: (7962,Species) :: ['carp']
