UNASSIGNED: @entity10397 ( @entity10397 ) is a histone chaperone that assembles acetylated histones H3/H4 onto newly synthesized DNA, allowing the de novo assembly of nucleosomes during replication. @entity10397 is an evolutionary conserved heterotrimeric protein complex. In @entity958 , the three @entity10397 subunits are encoded by @entity57600 , @entity57601 and @entity69546 . @entity57600 -4 mutants have reduced fertility due to a decrease in the number of cells that enter meiosis. Interestingly, the number of DNA double-strand breaks (DSBs), measured by scoring the presence of yH2AX, @entity57121 and @entity31342 foci, is higher than in @entity164 ( @entity164 ) plants, and meiotic recombination genes such @entity69547 / @entity69548 , @entity69549 , @entity57121 and @entity31342 are overexpressed. An increase in DSBs in this mutant does not have a significant effect in the mean chiasma frequency at metaphase I, nor a different number of @entity69550 nor @entity65651 foci per cell compared to @entity164 at pachytene. Nevertheless, this mutant does show a higher gene conversion (GC) frequency. To examine how an increase in DSBs influences meiotic recombination and synaptonemal complex (SC) formation, we analyzed double mutants defective for @entity57600 and different homologous recombination (HR) proteins. Most showed significant increases in both the mean number of synapsis initiation points (SIPs) and the total length of @entity69551 stretches in comparison with the corresponding single mutants. These experiments also provide new insight into the relationships between the recombinases in @entity958 , suggesting a prominent role for @entity31342 versus @entity57121 in establishing interhomolog interactions. In @entity958 an increase in the number of DSBs does not translate to an increase in the number of crossovers (COs) but instead in a higher GC frequency. We discuss different mechanisms to explain these results including the possible existence of CO homeostasis in plants.

Analysis of the Relationships between DNA Double-Strand Breaks, Synaptonemal Complex and Crossovers Using the XXXX -4 Mutant.

@entity10397 :: ('816521(Tax:3702)', 'Gene') :: ['CAF-1', 'Chromatin Assembly Factor 1'] @entity65651 :: ('829211(Tax:3702)', 'Gene') :: ['AtMUS81'] @entity69551 :: ('838831(Tax:3702)', 'Gene') :: ['AtZYP1'] @entity69548 :: ('816686(Tax:3702)', 'Gene') :: ['SAE2'] @entity958 :: ('3702', 'Species') :: ['Arabidopsis'] @entity69549 :: ('827854(Tax:3702)', 'Gene') :: ['AtBRCA1'] @entity69546 :: ('835935(Tax:3702)', 'Gene') :: ['MSI1'] @entity164 :: ('MESH:D017827', 'Disease') :: ['WT', 'wild-type'] @entity69547 :: ('824375(Tax:3702)', 'Gene') :: ['AtCOM1'] @entity57601 :: ('836584(Tax:3702)', 'Gene') :: ['FAS2'] @entity69550 :: ('826493(Tax:3702)', 'Gene') :: ['AtMLH1'] @entity57600 :: ('842858(Tax:3702)', 'Gene') :: ['FAS1', 'AtFAS1', 'Atfas1'] @entity31342 :: ('821860(Tax:3702)', 'Gene') :: ['AtDMC1'] @entity57121 :: ('832208(Tax:3702)', 'Gene') :: ['AtRAD51']
@entity57600 :: (842858(Tax:3702),Gene) :: ['Atfas1']
