OBJECTIVE: To define whether anti-ribosomal P (anti-P) autoantibodies from @entity1 with @entity12319 ( @entity12319 ) impair the function of hippocampal neurons that express the neuronal surface P antigen ( @entity68314 ) when accessing the brain via circulating blood. METHODS: We used anti-P antibodies from @entity1 with @entity12319 and rabbit-generated anti-P and anti- @entity68314 antibodies. Primary hippocampal neurons from @entity19 were analyzed to determine antibody cell surface binding (double immunofluorescence), intracellular calcium variations ( @entity593 AM), and apoptosis ( @entity185 activation). Hippocampal-dependent @entity2414 was assessed in @entity19 subjected to a water maze test 24 hours after an intravenous injection of anti-P or anti- @entity68314 , using lipopolysaccharide (LPS) to permeate the blood-brain barrier. Presence of antibodies and apoptosis in the hippocampus was studied using immunohistochemistry and TUNEL assays. RESULTS: Hippocampal neurons expressed @entity68314 on the cell surface, as revealed by anti-P and anti- @entity68314 staining colocalization, and responded to both anti-P and anti- @entity68314 by exhibiting increased intracellular @entity460 levels. Neuronal apoptosis was induced when anti-P was directly injected by stereotaxis into the hippocampus or added to primary cultures. Upon LPS treatment, intravenously injected anti-P impaired memory but did not elicit neuronal apoptosis in the hippocampus, where it was detectable in low amounts. Anti- @entity68314 antibodies also impaired memory. CONCLUSION: Anti-P antibodies interact with @entity68314 on the surface of hippocampal neurons leading to apoptotic death or to functional perturbations, results that are likely dependent on the concentration of these antibodies. Circulating anti-P can access the @entity2414 without requiring @entity528 when the blood-brain barrier is disrupted. @entity68314 can mediate antibody-driven diffuse @entity75 , and anti-P might contribute to the @entity73 that is frequently observed in @entity1252 .

Anti-ribosomal P protein autoantibodies from XXXX with @entity73 memory in @entity19 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity75 :: ('MESH:D001927', 'Disease') :: ['brain dysfunction'] @entity73 :: ('MESH:D003072', 'Disease') :: ['cognitive impairment'] @entity1252 :: ('MESH:D008180', 'Disease') :: ['SLE'] @entity185 :: ('12367(Tax:10090)', 'Gene') :: ['caspase 3'] @entity68314 :: ('195018(Tax:10090)', 'Gene') :: ['NSPA'] @entity593 :: ('MESH:D016257', 'Chemical') :: ['Fura 2'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity2414 :: ('MESH:D008569', 'Disease') :: ['spatial flexible memory', 'hippocampus and impair memory'] @entity528 :: ('MESH:D009410', 'Disease') :: ['neuronal death'] @entity12319 :: ('MESH:D020945', 'Disease') :: ['neuropsychiatric systemic lupus erythematosus', 'NPSLE']
@entity1 :: (9606,Species) :: ['patients']
