Telomerase activity was examined in @entity5 and compared with gene expression of the two core components of telomerase--the reverse transcriptase subunit ( @entity2215 ) and the RNA subunit ( @entity7066 )--and the proliferative index. We investigated 32 @entity5 across three to five sampled regions (20 @entity1653 , 1 @entity5513 , 1 @entity977 , 8 @entity11 , and 2 @entity2946 ). Telomerase activity was demonstrated by the telomeric repeat amplification protocol (TRAP) assay in seven (22%) @entity5 (four @entity1313 , two @entity1653 and @entity424 ) but could not be detected in the 18 (100%) @entity1653 . @entity2215 and @entity7066 mRNA were detected using reverse-transcription polymerase chain reaction (RT-PCR). @entity2215 mRNA was present in 20 (63%) @entity5 . Whereas @entity2215 mRNA transcripts were consistently low or absent in @entity1653 , @entity1313 exhibited elevated @entity2215 mRNAs. Multiple regions of @entity463 showed differences in telomerase activity and in the presence of @entity2215 mRNA. RT-PCR analysis revealed, for the first time in @entity5 , the presence of @entity2215 mRNA spliced products, corresponding to full-length mRNA as well as spliced mRNAs with critical reverse transcriptase motifs deleted. Only @entity5 with marked telomerase activity showed all @entity2215 spliced messages simultaneously. The absence of a positive correlation between telomerase activity and @entity2215 mRNA could not be attributed to the presence of @entity2215 spliced variants. We found a significant correlation between telomerase activity scores and Ki-67 proliferation index. A positive association is also seen between Ki-67 staining and the degree of @entity2215 mRNA expression. This shows that there seems to be a relationship between telomerase activity or the degree of @entity2215 expression and proliferation rate in @entity5 .

Telomerase activity and expression of telomerase reverse transcriptase correlated with cell proliferation in XXXX and @entity1313 in vivo.

@entity5513 :: ('MESH:D009464', 'Disease') :: ['acoustic schwannoma'] @entity7066 :: ('7012', 'Gene') :: ['hTR'] @entity424 :: ('MESH:D004806', 'Disease') :: ['one ependymoma'] @entity1313 :: ('MESH:D001932', 'Disease') :: ['malignant brain tumors'] @entity5 :: ('MESH:D009369', 'Disease') :: ['intracranial tumors', 'tumors'] @entity11 :: ('MESH:D005910', 'Disease') :: ['gliomas'] @entity1653 :: ('MESH:D008579', 'Disease') :: ['atypical meningiomas', 'benign meningiomas', 'meningiomas'] @entity977 :: ('MESH:D010911', 'Disease') :: ['pituitary adenoma'] @entity2215 :: ('7015', 'Gene') :: ['hTERT'] @entity463 :: ('MESH:D005909', 'Disease') :: ['glioblastomas'] @entity2946 :: ('MESH:D008527', 'Disease') :: ['medulloblastomas']
@entity1653 :: (MESH:D008579,Disease) :: ['meningiomas']
