UNASSIGNED: Lipopolysaccharide (LPS), the endotoxin of gram-negative bacteria, has detrimental effects on structure and function of the @entity291 corpus luteum (CL) in vivo. The objective was to investigate whether these effects were mediated directly by LPS or via LPS-induced release of PGF2a. @entity291 ovaries with a mid-cycle CL were collected immediately after slaughter and isolated perfused for 240 min. After 60 min of equilibration, LPS (0.5  g/mL) was added to the medium of five ovaries, whereas an additional six ovaries were not treated with LPS (control). After 210 min of perfusion, all ovaries were treated with 500 iu of hCG. In the effluent perfusate, concentrations of @entity1420 (P4) and @entity4126 a were measured every 10 and 30 min, respectively. Punch biopsies of the CL were collected every 60 min and used for RT-qPCR to evaluate mRNA expression of receptors for LPS ( @entity34919 ) and LH ( @entity64636 ), the cytokine @entity17299 , steroidogenic ( @entity27294 , @entity50161 ), angiogenic (VEGFA121, @entity12270 ) and vasoactive ( @entity40612 ) factors, and factors of @entity3009 synthesis (PGES, PGFS, @entity17439 ) and apoptosis ( @entity36388 , -8, -9). Treatment with LPS abolished the hCG-induced increase in P4 (P <=0.05); however, there was only a tendency (P=0.10) for increased release of @entity4126 a at 70 min after LPS challenge. Furthermore, mRNA abundance of @entity34919 , @entity17299 , @entity36388 , @entity68853 , PGES, PGFS, and VEGFA121 increased (P <=0.05) after LPS treatment, whereas all other factors remained unchanged (P>0.05). In conclusion, reduced P4 responsiveness to hCG in LPS-treated ovaries in vitro was not due to reduced steroidogenesis, but was attributed to enhanced apoptosis. However, an impact of luteal PGF2a could not be excluded.

Lipopolysaccharide enhances apoptosis of corpus luteum in isolated perfused XXXX ovaries in vitro.

@entity4126 :: ('782922(Tax:9913)', 'Gene') :: ['PGF2'] @entity68853 :: ('507481(Tax:9913)', 'Gene') :: ['CASP8'] @entity17299 :: ('280943(Tax:9913)', 'Gene') :: ['TNFA'] @entity1420 :: ('MESH:D011374', 'Chemical') :: ['progesterone'] @entity12270 :: ('281161(Tax:9913)', 'Gene') :: ['FGF2'] @entity36388 :: ('408016(Tax:9913)', 'Gene') :: ['CASP3'] @entity27294 :: ('281507(Tax:9913)', 'Gene') :: ['STAR'] @entity40612 :: ('281137(Tax:9913)', 'Gene') :: ['EDN1'] @entity3009 :: ('CHEBI:26333', 'Chemical') :: ['prostaglandin'] @entity50161 :: ('281824(Tax:9913)', 'Gene') :: ['HSD3B'] @entity34919 :: ('281534(Tax:9913)', 'Gene') :: ['TLR2, -4', 'TLR2'] @entity291 :: ('9913', 'Species') :: ['bovine', 'Bovine'] @entity64636 :: ('281900(Tax:9913)', 'Gene') :: ['LHCGR'] @entity17439 :: ('282020(Tax:9913)', 'Gene') :: ['PTGFR']
@entity291 :: (9913,Species) :: ['bovine']
