OBJECTIVE: To explore the relationship between CD44v6 expression, @entity876 gene mutation, cell @entity43 . METHODS: Eighty-five ovarian specimens (20 normal specimens, 20 @entity5 , 20 non- @entity189 and 25 @entity189 ) were collected. Cell ploidy and the distribution of cell population in each stage of cell cycle were detected by flow cytometry. By using techniques such as reverse transcription-polymerase chain reaction, southern blot and image analysis, the quantitative and qualitative expression of CD44v6 were determined; the mutation of @entity876 gene in the ovarian specimens was also determined by polymerase chain reaction-single strand conformation polymorphism with @entity575 staining. RESULTS: In the normal ovarian specimens, @entity5 , non-metastatic and @entity189 , the positive rates of CD44v6 expression and @entity876 gene mutation were 0%, 10%, 75%, 88% and 0%, 5%, 40%, 60%, respectively, both increased gradually. The mean dark density in the non-metastatic (3,820 +/- 289) and metastatic (10,132 +/- 1,521) groups was significantly different (P < 0.01). In the positive and negative CD44v6 @entity43 groups, the mean @entity189 cell percentage at the @entity11067 stage was 5.90% and 5.06% (P > 0.05), mean @entity189 cell percentage was 57% and 50% (P > 0.05) respectively. In the mutated and non-mutated @entity876 gene groups, their mean cell population at the @entity11067 stage was 11.15% and 5.85% (P < 0.05), mean @entity189 cell percentage was 74% and 36% (P < 0.05), respectively. CONCLUSIONS: The expression of CD44v6 is closely related to @entity189 @entity3 . The relationship between CD44v6 expression, @entity876 gene mutation and @entity189 @entity3 can be involved in the different mechanism. As compared to mutated @entity876 gene, CD44v6 appears to be more suitable as a metastatic marker.

[Study on the relationship between CD44v6, XXXX gene mutation and @entity43 @entity3 ].

@entity43 :: ('MESH:D010051', 'Disease') :: ['ovarian carcinoma', 'ploidy and ovarian metastasis'] @entity11067 :: ('p|SUB|G|2|M', 'ProteinMutation') :: ['G2M'] @entity575 :: ('MESH:D012834', 'Chemical') :: ['silver'] @entity5 :: ('MESH:D009369', 'Disease') :: ['benign tumors'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinoma', 'metastatic carcinomas', 'aneuploidy carcinoma'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis']
@entity876 :: (7157,Gene) :: ['p53']
