UNASSIGNED: @entity1371 (low serum @entity972 level) is a common electrolyte imbalance that can cause a defect in urinary concentrating ability, i.e., @entity3040 ( @entity3040 ), but the molecular mechanism is unknown. We employed proteomic analysis of inner medullary collecting ducts (IMCD) from @entity35 fed with a @entity972 -free diet for 1 day. IMCD protein quantification was performed by mass spectrometry using a label-free methodology. A total of 131 proteins, including the water channel @entity14354 , exhibited significant changes in abundance, most of which were decreased. Bioinformatic analysis revealed that many of the down-regulated proteins were associated with the biological processes of generation of precursor metabolites and energy, actin cytoskeleton organization, and cell-cell adhesion. @entity15 and immunoblotting studies further confirmed the down regulation of 18 selected proteins. Electron microscopy showed autophagosomes/autophagolysosomes in the IMCD cells of @entity35 deprived of @entity972 for only 1 day. An increased number of autophagosomes was also confirmed by immunofluorescence, demonstrating co-localization of @entity5615 and @entity20350 with @entity14354 and several other down-regulated proteins in IMCD cells. @entity14354 was also detected in autophagosomes in IMCD cells of @entity972 -deprived @entity35 by immunogold electron microscopy. Thus, enhanced autophagic degradation of proteins, most notably including @entity14354 , is an early event in @entity1371 -induced @entity3040 .

Autophagic degradation of XXXX is an early event in @entity1371 -induced @entity3040 .

@entity5615 :: ('25291(Tax:10116)', 'Gene') :: ['LC3'] @entity14354 :: ('25386(Tax:10116)', 'Gene') :: ['AQP2'] @entity20350 :: ('25328(Tax:10116)', 'Gene') :: ['Lamp1'] @entity972 :: ('MESH:D011188', 'Chemical') :: ['potassium'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity15 :: ('MESH:D009103', 'Disease') :: ['Targeted LC-MS/MS'] @entity3040 :: ('MESH:D018500', 'Disease') :: ['NDI', 'nephrogenic diabetes insipidus'] @entity1371 :: ('MESH:D007008', 'Disease') :: ['hypokalemia', 'Hypokalemia']
@entity14354 :: (25386(Tax:10116),Gene) :: ['aquaporin-2']
