BACKGROUND: It has recently been reported that sulindac has an apoptotic effect on KYN-2 cells, an undifferentiated @entity157 cell line. The present work investigates whether sulindac also has an apoptotic effect on well-differentiated @entity157 cells and what its potential mechanism might be. MATERIALS AND METHODS: Hep G2 cells were treated with sulindac at different concentrations. Apoptosis rate, cell proliferation and @entity665 incorporation were measured. The activities of @entity522 , acid and neutral sphingomyelinase and the changes of @entity4291 content were also assayed. RESULTS: Sulindac dose-dependently induced apoptosis in Hep G2 cells; both sulindac @entity5879 and @entity3287 had similar effects. The apoptosis was accompanied by an increase of @entity522 activity and a decrease of cell proliferation and @entity665 incorporation. No significant change could be observed for the activity of sphingomyelinase and @entity4291 content. CONCLUSION: Sulindac induces apoptosis and inhibits proliferation in Hep G2 cells. The effect may be mediated by a pathway related to @entity522 activation but independent of @entity4291 metabolism

Sulindac induces apoptosis, inhibits proliferation and activates XXXX in Hep G2 cells.

@entity5879 :: ('CHEBI:35850', 'Chemical') :: ['sulfone'] @entity522 :: ('836', 'Gene') :: ['caspase-3'] @entity665 :: ('CHEBI:53526', 'Chemical') :: ['3H-thymidine'] @entity157 :: ('MESH:D006528', 'Disease') :: ['hepatoma'] @entity3287 :: ('CHEBI:26822', 'Chemical') :: ['sulfide'] @entity4291 :: ('CHEBI:17636', 'Chemical') :: ['sphingomyelin']
@entity522 :: (836,Gene) :: ['caspase-3']
