BACKGROUND: The second messengers @entity669 kinase (TK) and @entity7131 ( @entity7131 ) have been implicated in mediating the cellular signaling cascade during @entity99 preconditioning (IPC). We evaluated the role of TK and @entity7131 on the modulation of the transcription factor nuclear factor kappa B (NFkappaB) and its inhibitor @entity39441 during IPC. STUDY DESIGN: Yorkshire @entity1707 underwent routine harvest. IPC livers underwent 15 minutes of @entity63 and 15 minutes of in situ perfusion before harvest, with or without pretreatment with a TK inhibitor ( @entity670 ) or a @entity7131 inhibitor ( @entity668 ). During cold storage and reperfusion, tissue extracts were analyzed for @entity39441 phosphorylation and NFkappaB levels and for TK and @entity7131 activity by Western blot. RESULTS: Control @entity1707 livers demonstrated no change in the levels of TK, @entity7131 , @entity39441 , or NFkappaB before @entity63 . IPC grafts demonstrated activation of TK and @entity7131 with increased @entity39441 phosphorylation and NFkappaB levels before @entity63 . IPC grafts pretreated with @entity670 demonstrated inhibition of TK activation but not of @entity7131 activation. @entity670 -pretreated grafts also demonstrated inhibition of @entity39441 phosphorylation and a lack of NFkappaB translocation to the nucleus throughout the entire experiment. IPC grafts pretreated with @entity668 demonstrated inhibition of @entity7131 activation but not TK activation. @entity668 -pretreated grafts also demonstrated @entity39441 phosphorylation before @entity63 and enhanced nuclear levels of NFkappaB. CONCLUSIONS: Data suggest that the role of TK in IPC might be mediated in part by NFkappaB, but @entity7131 does not depend on NFkappaB for its effect. Two parallel signaling pathways might explain these data.

Regulation of NFkappaB in XXXX preconditioning.

@entity1707 :: ('9823', 'Species') :: ['pigs', 'pig'] @entity99 :: ('MESH:D056486', 'Disease') :: ['hepatic ischemic'] @entity63 :: ('MESH:D007511', 'Disease') :: ['cold ischemia', 'ischemia'] @entity670 :: ('MESH:D019833', 'Chemical') :: ['genistein', 'Genistein'] @entity668 :: ('MESH:C016299', 'Chemical') :: ['Chelerythrine', 'chelerythrine'] @entity7131 :: ('MESH:D020151', 'Disease') :: ['protein kinase C', 'PKC'] @entity39441 :: ('406188(Tax:9823)', 'Gene') :: ['IkappaB alpha'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine']
@entity99 :: (MESH:D056486,Disease) :: ['hepatic ischemic']
