Formation of oligosaccharides occurs both in the cytosol and in the lumen of the endoplasmic reticulum (ER). Luminal oligosaccharides are transported into the cytosol to ensure that they do not interfere with proper functioning of the glycan-dependent quality control machinery in the lumen of the ER for newly synthesized glycoproteins. Once in the cytosol, free oligosaccharides are catabolized, possibly to maximize the reutilization of the component @entity3363 . An endo- @entity10814 ( @entity68945 ) is a key enzyme involved in the processing of free oligosaccharides in the cytosol. This enzyme activity has been widely described in animal cells, but the gene encoding this enzyme activity has not been reported. Here, we report the identification of the gene encoding @entity1 cytosolic @entity68945 . After 11 steps, the enzyme was purified 150,000-fold to homogeneity from hen oviduct, and several internal @entity535 sequences were analyzed. Based on the internal sequence and examination of expressed sequence tag (EST) databases, we identified the @entity1 orthologue of the purified protein. The @entity1 protein consists of 743 aa and has no apparent signal sequence, supporting the idea that this enzyme is localized in the cytosol. By expressing the cDNA of the putative @entity1 @entity68945 in COS-7 cells, the enzyme activity in the soluble fraction was enhanced 100-fold over the basal level, confirming that the @entity1 gene identified indeed encodes for @entity68945 . Careful gene database surveys revealed the occurrence of @entity68945 homologues in @entity238 , @entity2199 , and @entity958 , indicating the broad occurrence of @entity68945 in higher eukaryotes. This gene was expressed in a variety of @entity1 tissues, suggesting that this enzyme is involved in basic biological processes in eukaryotic cells.

Endo- XXXX , an enzyme involved in processing of free oligosaccharides in the cytosol.

@entity1 :: ('9606', 'Species') :: ['human'] @entity2199 :: ('6239', 'Species') :: ['Caenorhabditis elegans'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity958 :: ('3702', 'Species') :: ['Arabidopsis thaliana'] @entity10814 :: ('10724', 'Gene') :: ['beta-N-acetylglucosaminidase'] @entity238 :: ('7227', 'Species') :: ['Drosophila melanogaster'] @entity68945 :: ('64772', 'Gene') :: ['ENGase'] @entity3363 :: ('MESH:D002241', 'Chemical') :: ['sugars']
@entity10814 :: (10724,Gene) :: ['beta-N-acetylglucosaminidase']
