UNASSIGNED: Toll-like receptors (TLRs) recognize pathogen-derived molecules and play a critical role during the host innate and adaptive immune response. Brucella spp. are intracellular gram-negative bacteria including several virulent species, which cause a chronic @entity2927 in a wide range of @entity1 hosts known as @entity3377 . A new Brucella species, @entity25907 , was recently isolated from wild rodents and found to be highly pathogenic in @entity19 . Using this species-specific model, it was previously found that CD8(+) T cells are required to control this @entity281 . In order to find out the role of TLR-mediated responses in the control of this pathogen, the course of @entity281 of @entity25907 was analyzed over 3 weeks in wild-type (WT) and TLR knock out (KO) @entity19 including @entity8932 (-/-), @entity1990 (-/-), @entity14540 (-/-), @entity8932 *4(-/-) and @entity8932 *4*9(-/-). WT and single @entity8932 , @entity1990 and @entity14540 KO @entity19 similarly control @entity281 in liver and spleen. In contrast, bacterial clearance was delayed in @entity8932 *4(-/-) and @entity8932 *4*9(-/-) @entity19 at 7 and 14 days post- @entity281 . This defect correlated with impaired maturation and pro-inflammatory cytokine production in @entity25907 -infected dendritic cells from @entity8932 *4(-/-) and @entity8932 *4*9(-/-) @entity19 . Finally, it was found that Tc cells from @entity8932 *4(-/-) and @entity8932 *4*9(-/-) @entity19 showed reduced ability to inhibit growth of @entity25907 in macrophages, suggesting the involvement of @entity8932 and 4 in the generation of specific Tc cells. Our findings indicate that @entity8932 and @entity1990 are required to control @entity25907 @entity281 in @entity19 and that this effect could be related to its participation in the maturation of dendritic cells and the generation of specific CD8(+) Tc cells.

Toll-Like Receptors 2 and 4 Cooperate in the Control of the Emerging Pathogen XXXX .

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity25907 :: ('444163', 'Species') :: ['B. microti', 'Brucella microti'] @entity3377 :: ('MESH:D002006', 'Disease') :: ['brucellosis'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity2927 :: ('MESH:D015047', 'Disease') :: ['zoonotic infection'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity8932 :: ('24088(Tax:10090)', 'Gene') :: ['TLR2'] @entity1990 :: ('21898(Tax:10090)', 'Gene') :: ['TLR4'] @entity14540 :: ('81897(Tax:10090)', 'Gene') :: ['TLR9']
@entity25907 :: (444163,Species) :: ['Brucella microti']
