To study hepatocyte injure through establishing the @entity35 model of @entity879 (ALF). ALF @entity35 model was established by administration with @entity8688 and @entity558 , and then giving lowering blood @entity3265 (LBA) treatment. Besides, the intervention groups were injected with @entity7222 and @entity5906 . The control groups were injected saline. Blood @entity3265 , ALT, AST, @entity1505 and @entity568 in blood samples were test at 12 hrs and 24 hrs after treatment with LBA. Hepatocyte apoptosis were tested by TUNEL and DNA Ladder. Expression of @entity10740 and @entity32351 were detected by RT-PCR. RESULTS: showed that blood @entity3265 in @entity1578 group and intervention group compared with blank control group was significantly increased at 12 h, 24 h; intervention group compared with @entity1578 group was significantly reduced (P<0.05). Serum ALT, AST in 24 h group were higher than 12 h. 12 h intervention group was decreased compared with @entity1578 group, but there was no significant statistically difference (P>0.05). 24 h intervention group compared with @entity1578 group was significantly reduced (P<0.05). Except the control group, DNA ladder and the TUNEL results showed hepatocyte apoptosis rate increased in 24 h compared with 12 h. Intervention group compared with @entity1578 group was significantly reducing (P<0.05). @entity568 , @entity1505 , @entity10740 expression levels were increased with time (24 h>12 h). The @entity1578 and intervention group compared with blank control group was significantly increased; Intervention group compared with @entity1578 group was significantly reducing (P<0.05). @entity32351 gene was high expression in ALF @entity35 model. @entity32351 level in @entity1578 and intervention group compared with control group was significantly increased, and intervention group compared with @entity1578 group was significantly reducing (P<0.05). In conclusion, hepatocyte apoptosis is an important pathological change in ALF @entity35 mode, and lowing @entity3265 can reduce @entity99 and apoptosis. Blood @entity1505 , @entity568 and @entity32351 may be more sensitive injure indicators.

Lowering blood XXXX prevents hepatocyte injury and apoptosis.

@entity10740 :: ('301300(Tax:10116)', 'Gene') :: ['p53', 'P53'] @entity558 :: ('MESH:C536528', 'Disease') :: ['LPS'] @entity3265 :: ('MESH:D000641', 'Chemical') :: ['ammonia'] @entity879 :: ('MESH:D017114', 'Disease') :: ['acute hepatic failure'] @entity568 :: ('24498(Tax:10116)', 'Gene') :: ['IL-6'] @entity5906 :: ('D001224', 'Chemical') :: ['aspartate'] @entity1505 :: ('24835(Tax:10116)', 'Gene') :: ['TNF-a'] @entity7222 :: ('CHEBI:18257', 'Chemical') :: ['ornithine'] @entity1578 :: ('MESH:D017093', 'Disease') :: ['hepatic failure'] @entity8688 :: ('CHEBI:60313', 'Chemical') :: ['D-galactosamine'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity99 :: ('MESH:D056486', 'Disease') :: ['liver injury'] @entity32351 :: ('25353(Tax:10116)', 'Gene') :: ['SPP1']
@entity3265 :: (MESH:D000641,Chemical) :: ['ammonia']
