It has been argued on both evolutionary and functional grounds that genes must be shared by the @entity1 sex chromosomes. The only direct evidence for such genes is our previous finding that loci on the @entity1 X ( @entity3778 ) and Y ( @entity3778 ) chromosomes encode a species-specific @entity3058 recognised by the monoclonal antibody 12E7. These loci map to the regions of the sex chromosomes which pair at meiosis, and @entity3778 has been shown to escape X-inactivation. We have used immunoprecipitation and Western blot analysis combined with one- and two-dimensional @entity2975 gel electrophoresis to compare the products of @entity3778 and @entity3778 . The @entity1 specific molecule recognised by the 12E7 antibody is a membrane-associated polypeptide of mol. wt. 32.5 kd and pI = 5.0. No difference in size or charge has been detected between X and Y encoded forms of this molecule confirming that @entity3778 is a functional homologue of @entity3778 . An intracellular polypeptide of mol. wt. 29 kd and pI = 7.0 present in the cytoplasm of both @entity1 and @entity19 cells is also recognised by the 12E7 antibody.

Biochemical analysis of an antigen produced by both XXXX sex chromosomes.

@entity1 :: ('9606', 'Species') :: ['mammalian', 'human'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity3058 :: ('963', 'Gene') :: ['cell surface antigen'] @entity3778 :: ('4267', 'Gene') :: ['MIC2X', 'MIC2Y'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide']
@entity1 :: (9606,Species) :: ['human']
