Recently, developing drug delivery systems exhibiting controlled drug release at the @entity5 sites emerged as an attractive option for enhancing anticancer therapeutic efficacy. It seems, however, unlikely that single agent therapies will prove effective enough against the myriad cells present within the @entity5 . Therefore, next generation nanotherapeutics must not only find their way to the solid @entity5 but also must effectively destroy the diverse populations of cells promoting @entity5 growth. Nanoliposomes offer an important advantage in the delivery of a combination of drugs acting synergistically in @entity5 treatment. They allow controlling the pharmacokinetics and biodistribution of the drugs by uniform time and spatial co-delivery of the agents. However, successful translation of such complex formulations into the clinic relies on understanding critical physicochemical characteristics. These include: liposomes' membrane phase and dynamics, size distribution, state of encapsulated drug, internal environment of liposome, state of grafted @entity968 at the liposome surface, and in-vivo drug release rate. They determine the pharmacokinetics of the formulation and the bioavailability of the drugs. We encapsulated the combination of @entity1891 (VCR) and topotecan ( @entity9919 ) in the same nanoliposome (LipoViTo). Our in-vitro and in-vivo characterization of LipoViTo provides an explanation for the good therapeutic efficacy that was previously reported by us. Moreover, we have described how to study these critical features for a two-drug in one nanoliposome formulation. This characterization is an important step for a rational clinical development and for how to ensure liposome product quality of LipoViTo and other liposomal formulations alike.

Characterization of PEGylated nanoliposomes co-remotely loaded with topotecan and XXXX : relating structure and pharmacokinetics to therapeutic efficacy.

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'malignancy'] @entity9919 :: ('MESH:C026677', 'Chemical') :: ['TPT'] @entity1891 :: ('MESH:D014750', 'Chemical') :: ['vincristine'] @entity968 :: ('MESH:D011092', 'Chemical') :: ['polyethylene glycol']
@entity1891 :: (MESH:D014750,Chemical) :: ['vincristine']
