UNASSIGNED: The bacterium @entity6464 is the causative agent of @entity6690 , a systemic, ubiquitous disease of fish in the salmon family, characterized by high mortality and morbidity. Probiotics are a promising approach for prevention of @entity6690 in aquaculture. A bacterial strain with anti-A. salmonicida properties, @entity10679 V4, was isolated and the mechanisms underlying these properties were investigated. Anti-A. salmonicida compounds present in cell-free supernatant of V4 were purified and structurally identified as members of the iturin, macrolactin, and @entity68872 groups. The compounds contributed jointly to inhibition of A. salmonicida, and the diversity of the compounds was related to the versatility of their mode of action. Addition of the compounds to A. salmonicida cell suspensions reduced cell density. Analyses by confocal microscopy and scanning electron microscopy revealed cell membrane disruption, deletion of cellular content, and cell lysis of A. salmonicida. The V4 genome was sequenced, and gene clusters involved in synthesis of anti-Aeromonas compounds were detected and identified. A possible probiotic effect on growth performance of @entity5660 ( @entity5660 ) was investigated by addition of 0, 1, and 3  % (v/w) V4. Relative to control, mortality was reduced 27.25  % in the 1  % addition group and 81.86  % in the 3  % addition group. Feed coefficient ratio was reduced 19.49  % and weight gain ratio was increased 71.22  % in the 1  % addition group. Our findings demonstrate that V4 is an effective probiotic strain in @entity5660 and has clear potential for both control of @entity6690 and growth promotion of aquaculture animals.

Characterization and mechanism of anti-Aeromonas salmonicida activity of a marine probiotic strain, XXXX V4.

@entity5660 :: ('8022', 'Species') :: ['O. mykiss', 'Oncorhynchus mykiss', 'rainbow trout'] @entity10679 :: ('1390', 'Species') :: ['Bacillus velezensis'] @entity6464 :: ('645', 'Species') :: ['Aeromonas salmonicida'] @entity6690 :: ('MESH:D005667', 'Disease') :: ['furunculosis'] @entity68872 :: ('MESH:C054843', 'Chemical') :: ['difficidin']
@entity10679 :: (1390,Species) :: ['Bacillus velezensis']
