Electrophoretic conditions to separate @entity3208 ( @entity214 )-protein complexes according to their relative molecular mass by capillary electrophoresis (CE) using linear @entity2975 as a sieving matrix were examined. Five purified proteins with relative molecular masses between 14 400 and 66 200 Da were separated on a coated fused- @entity3440 capillary with an internal diameter of 100 microm and an effective length of 24 cm (total length, 32.5 cm). @entity13266 was added to the solution of purified proteins as internal standard; @entity5643 was also added as reducing agent. The running buffer composition was 0.05 M @entity8973 (Tris), 0.035 M @entity2574 , 0.1% m/v @entity214 , 4% m/v @entity3323 , the resulting pH being 8.0. The applied voltage was 7 kV (reversed voltage polarity) in order to avoid high current intensities. Under optimized conditions, the five proteins were separated in less than 15 min, with a % relative standard deviation (RSD) between 0.2 and 0.4 for migration times in the same day. Good efficiency (values between 150 000 and 40 000 N/m) and resolution (values between 2 and 2.8) were obtained. The inverse of relative migration times was found to correlate with the logarithm of their relative molecular mass. Finally, cider proteins were analyzed and their relative molecular masses were determined. These results were compared with those obtained by @entity214 - @entity2975 gel electrophoresis ( @entity214 -PAGE).

Size-based separations of proteins by capillary electrophoresis using linear XXXX as a sieving medium: model studies and analysis of cider proteins.

@entity13266 :: ('MESH:D019817', 'Chemical') :: ['Benzoic acid'] @entity3208 :: ('MESH:D012967', 'Chemical') :: ['sodium dodecyl sulfate'] @entity3323 :: ('MESH:D020106', 'Chemical') :: ['acrylamide'] @entity8973 :: ('MESH:D014325', 'Chemical') :: ['tris(hydroxymethyl)aminomethane'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity3440 :: ('MESH:D012822', 'Chemical') :: ['silica'] @entity5643 :: ('CHEBI:41218', 'Chemical') :: ['beta-mercaptoethanol'] @entity2574 :: ('CHEBI:22660', 'Chemical') :: ['aspartic acid'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS']
@entity2975 :: (CHEBI:51135,Chemical) :: ['polyacrylamide']
