OBJECTIVE: To investigate the alteration of oncogenes and @entity5 suppressor genes in @entity356 . METHODS: Southern blot, PCR/SSCP and DNA sequencing techniques were used in 33 @entity356 to detect @entity1916 , @entity470 , @entity1722 , @entity23698 , c-Ha-ras, @entity876 , @entity986 and @entity18962 , for the presence of amplification, deletion, mutation and rearrangement. RESULTS: Most @entity5 (70%) haboured one or more altered genes. The number and type of gene alteration were different among individuals. Rearrangement of @entity1916 was noted in 2/33 cases (6%), and amplification of @entity1916 , @entity1722 and @entity23698 in 8/33 cases (24.2%), 1/33 cases (3%) and 2/18 cases (11%) respectively. Homozygous deletion of @entity986 was seen in 6/33 cases (18%). Loss of heterozygousity was also noted in @entity18962 5/17 (29%) and @entity876 2/13 (16%). The mutation rate of @entity876 in exon 5-8 was 20/33 (61%). Point mutation of @entity876 was found at both early and advanced @entity5 . In contrast, amplification of oncogenes and loss of @entity5 suppressor genes were correlated with poorly differentiated and @entity3 . CONCLUSIONS: @entity356 is a gradually developed process, results from sequencial alteration of multigenes. The malignant phenotype is associated with the degree of @entity74 .

[Correlation of multiple gene changes with malignant phenotype of human XXXX ].

@entity986 :: ('1029', 'Gene') :: ['P16', 'p16'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity470 :: ('1956', 'Gene') :: ['EGFR'] @entity18962 :: ('4830', 'Gene') :: ['nm23-H1'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity1916 :: ('4233', 'Gene') :: ['c-met'] @entity23698 :: ('208', 'Gene') :: ['AKT-2'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastatic tumors'] @entity74 :: ('MESH:D030342', 'Disease') :: ['genetic abnormality'] @entity356 :: ('MESH:D013274', 'Disease') :: ['Gastric carcinogenesis', 'gastric carcinogenesis', 'gastric carcinomas'] @entity1722 :: ('2064', 'Gene') :: ['c-erbB-2']
@entity356 :: (MESH:D013274,Disease) :: ['gastric carcinoma']
