@entity14782 ( @entity14782 ) is a novel approach for delivering drugs directly into @entity1313 by intracranial infusion, enabling the distribution of high drug concentrations over large tissue volumes. This study was designed to present a method for binding @entity1499 ( @entity1500 ) to unique crystalline, highly ordered and superparamagnetic maghemite nanoparticles via human serum albumin (HSA) coating, optimized for @entity14782 treatments of @entity11 . Naked nanoparticles and HSA- or @entity968 ( @entity970 )-coated nanoparticles with/without @entity1500 were studied. In vitro results showed no @entity137 and a similar cell-kill efficacy of the @entity1500 -loaded particles via HSA coating to that of free @entity1500 , while @entity1500 -loaded particles via @entity970 coating showed low efficacy. In vivo, the @entity970 -coated nanoparticles provided the largest distributions in normal @entity35 brain and long clearance times, but due to their low efficacy in vitro, were not considered optimal. The naked nanoparticles provided the smallest distributions and shortest clearance times. The HSA-coated nanoparticles (with/without @entity1500 ) provided good distributions and long clearance times (nearly 50% of the distribution volume remained in the brain 3 weeks post treatment). No @entity1500 -related @entity137 was noted. These results suggest that the formulation in which HSA was bound to our nanoparticles via a unique precipitation method, and @entity1500 was bound covalently to the HSA, could enable efficient and stable drug loading with no apparent @entity137 . The cell-kill efficacy of the bound @entity1500 remained similar to that of free @entity1500 , and the nanoparticles presented efficient distribution volumes and slow clearance times in vivo, suggesting that these particles are optimal for @entity14782 .

@entity14782 of XXXX -loaded maghemite nanoparticles.

@entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity1313 :: ('MESH:D001932', 'Disease') :: ['brain tumors'] @entity14782 :: ('MESH:C564835', 'Disease') :: ['Convection-enhanced delivery', 'CED'] @entity11 :: ('MESH:D005910', 'Disease') :: ['gliomas'] @entity1500 :: ('CHEBI:44185', 'Chemical') :: ['MTX'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity1499 :: ('MESH:D008727', 'Chemical') :: ['methotrexate'] @entity970 :: ('CHEBI:46793', 'Chemical') :: ['PEG'] @entity968 :: ('MESH:D011092', 'Chemical') :: ['polyethylene glycol']
@entity1499 :: (MESH:D008727,Chemical) :: ['methotrexate']
