Transplantation of @entity2690 (INS)-secreting cells differentiated in vitro from stem cells promises a safer and easier treatment of severe @entity6 . A volatile bioactive compound, @entity3957 ( @entity3958 ), promotes cell differentiation; @entity1 tooth-pulp stem cells undergo hepatic differentiation. The aim of this study is to develop a novel protocol using @entity3958 to enhance pancreatic differentiation from the CD117(+) cell fraction of @entity1 tooth pulp. During the differentiation, the cells were exposed to 0.1   ng   ml(-1) @entity3958 . Immunocytochemistry, RT-PCR, determination of INS c-peptide content and flow cytometry of pancreatically related markers were carried out. Expression of WNT and the PI3K/AKT signaling pathway were also determined by PCR array. After differentiation, INS, glucagon ( @entity5445 ), @entity1943 (SST) and @entity3770 (PPY) were positive when examined by immunofluorescence. INS and @entity5445 were also determined flow-cytometrically. Only the cells expressing INS increased after @entity3958 exposure. The number of cells expressing @entity5445 was significantly decreased. Genes involved in canonical WNT and the WNT/ @entity460 pathways were highly expressed after @entity3958 exposure. @entity3958 accelerated INS synthesis and secretion by regenerated INS-producing cells from @entity1 teeth. All signaling pathway functions of the PI3K-AKT pathway were extremely activated by @entity3958 exposure. The matured INS-producing cells originating in @entity1 teeth were increased by @entity3958 in order to control blood- @entity413 level.

Regeneration of @entity2690 -producing pancreatic cells using a volatile bioactive compound and XXXX teeth.

@entity3958 :: ('CHEBI:36945', 'Chemical') :: ['H2S'] @entity1 :: ('9606', 'Species') :: ['human'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetes mellitus'] @entity2690 :: ('3630', 'Gene') :: ['insulin'] @entity3770 :: ('5539', 'Gene') :: ['pancreatic polypeptide'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity1943 :: ('MESH:D013004', 'Chemical') :: ['somatostatin'] @entity3957 :: ('MESH:D006862', 'Chemical') :: ['hydrogen sulfide'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity5445 :: ('2641', 'Gene') :: ['GCG']
@entity1 :: (9606,Species) :: ['human']
