This study was undertaken to investigate electrophysiological properties of immortalized SV40-transfected @entity1 corneal endothelial cells (HCEC-SV40) combined with the analysis of intracellular @entity141 responses mediated by ligands for receptor @entity669 kinases (RTK). In addition, the effects of several @entity669 kinase inhibitors were tested on @entity141 inflow mediated by induction of capacitative @entity460 entry (CCE). Patch-clamp techniques and measurements of the intracellular free @entity141 ([ @entity141 ](i)) by @entity593 were performed using HCEC-SV40. Stimulation of fibroblast growth factor receptors (FGFR) (e.g. by basic-FGF) (10 ng ml(-1)) elicited activation of @entity141 permeable channels and a subsequent increase of cytosolic free @entity141 in HCEC-SV40. This effect could be disrupted by the L-type @entity141 channel blocker @entity594 (5 microM). In addition, @entity594 significantly reduced the magnitude of CCE. Inhibition of protein @entity669 kinases (PTKs) by @entity670 , @entity10755 , or @entity12079 51 (all 5 microM) also led to a reduction of CCE in HCEC-SV40. This study demonstrates for the first time that L-type @entity141 channel activity in HCEC-SV40 is linked to the activity of FGF receptor @entity669 kinases. These data regarding @entity141 inflow through @entity141 channels could be useful for investigation of culture and vitality conditions of HCEC.

@entity460 influx induced by activation of receptor @entity669 kinases in SV40-transfected XXXX corneal endothelial cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity670 :: ('MESH:D019833', 'Chemical') :: ['genistein'] @entity10755 :: ('MESH:C062898', 'Chemical') :: ['lavendustin A'] @entity593 :: ('MESH:D016257', 'Chemical') :: ['fura-2'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity594 :: ('MESH:D009543', 'Chemical') :: ['nifedipine'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity12079 :: ('CHEBI:38637', 'Chemical') :: ['tyrphostin'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine']
@entity1 :: (9606,Species) :: ['human']
