KEY MESSAGE: Extensive modulation of numerous @entity4059 transcripts in the embryogenic culture of Arabidopsis indicates a substantial role of auxin signaling in the mechanism of somatic embryogenesis induction. @entity2531 ( @entity2531 ) is induced by auxin in plants and auxin signaling is considered to play a key role in the molecular mechanism that controls the embryogenic transition of plant somatic cells. Accordingly, the expression of @entity4059 ( @entity4059 ) genes in embryogenic culture of Arabidopsis was analyzed. The study revealed that 14 of the 22 ARFs were transcribed during @entity2531 in Arabidopsis. RT-qPCR analysis indicated that the expression of six ARFs (ARF5, ARF6, ARF8, ARF10, ARF16, and ARF17) was significantly up-regulated, whereas five other genes (ARF1, ARF2, ARF3, ARF11, and ARF18) were substantially down-regulated in the @entity2531 -induced explants. The activity of ARFs during @entity2531 was also monitored with GFP reporter lines and the ARFs that were expressed in areas of the explants engaged in @entity2531 induction were detected. A functional test of ARFs transcribed during @entity2531 was performed and the embryogenic potential of the arf mutants and overexpressor lines was evaluated. ARFs with a significantly modulated expression during @entity2531 coupled with an impaired embryogenic response of the relevant mutant and/or overexpressor line, including ARF1, ARF2, ARF3, ARF5, ARF6, ARF8, and ARF11 were indicated as possibly being involved in @entity2531 induction. The study provides evidence that embryogenic induction strongly depends on ARFs, which are key regulators of the auxin signaling. Some clues on the possible functions of the candidate ARFs, especially ARF5, in the mechanism of embryogenic transition are discussed. The results provide guidelines for further research on the auxin-related functional genomics of @entity2531 and the developmental plasticity of somatic cells.

Expression profiling of XXXX genes during somatic embryogenesis induction in Arabidopsis.

@entity2531 :: ('MESH:D013001', 'Disease') :: ['SE', 'Somatic embryogenesis'] @entity4059 :: ('MESH:D007020', 'Disease') :: ['AUXIN RESPONSE FACTOR', 'ARF']
@entity4059 :: (MESH:D007020,Disease) :: ['AUXIN RESPONSE FACTOR']
