NO was detected in @entity291 trabecular meshwork (TM). @entity291 eyes were perfused (posterior ciliary artery). In some eyes (operated eyes) a NO electrode was inserted adjacent to the TM (scleral flap). Vascular perfusion/intraocular pressures (VPP/IOP) were recorded. In operated eyes, @entity1425 (1 nM-100 microM) increased NO (maximally 979.9 +/- 117.6 nM, mean +/- @entity2832 ). @entity6839 (1 mM) shifted the @entity1425 -NO concentration-response curve rightward (2.94 log units) without significantly changing the maximal response (903.0 +/- 67.7 nM, mean +/- @entity2832 ). The non-selective NO synthase (NOS) inhibitor @entity7293 (100 microM) virtually abolished the NO response to @entity1425 . @entity7293 alone (1 microM-100 microM) significantly reduced tonic NO generation (maximally 109.5 +/- 24.9 nM, mean +/- @entity2832 ), whereas @entity6839 alone (1 microM-1 mM) had no effect. In unoperated eyes, @entity1425 (1 nM-100 microM) reduced IOP (maximally 2.56 +/- 0.64 mmHg, mean +/- @entity2832 ). @entity1425 (100 microM) mildly increased VPP (4.6 +/- 1.3 mmHg, mean +/- @entity2832 ). Baseline @entity7462 formation rate (11.5 +/- 3.2 microl/min, mean +/- @entity2832 ) was unaffected. Effluent perfusate (effusate) total @entity6836 was determined by enzymatically reducing all @entity8326 to @entity6836 , then assessing resultant @entity6836 (Griess assay). @entity1425 (1 nM-1 microM) increased effusate @entity6836 (maximally 15.8 +/- 4.9 microM, mean +/- @entity2832 ). @entity6839 (1 mM) reduced, and @entity7293 (100 microM) virtually abolished effusate @entity6836 response to @entity1425 . @entity7293 alone (1 microM-100 microM) reduced tonic effusate @entity6836 (maximally from 5.8 +/- 1.6 microM to 1.1 +/- 0.9 microM, mean +/- @entity2832 ), whereas @entity6839 alone (1 microM-1 mM) had no effect. NO is generated tonically in @entity291 TM and increases in response to @entity1425 .

Real-time direct measurement of nitric oxide in XXXX perfused eye trabecular meshwork using a clark-type electrode.

@entity8326 :: ('CHEBI:29794', 'Chemical') :: ['NO(3)(-)'] @entity6839 :: ('MESH:D013999', 'Chemical') :: ['timolol', 'Timolol'] @entity1425 :: ('MESH:D004837', 'Chemical') :: ['Epinephrine', 'epinephrine'] @entity7462 :: ('MESH:C562390', 'Disease') :: ['aqueous humor'] @entity7293 :: ('CHEBI:28229', 'Chemical') :: ['L-NMMA'] @entity6836 :: ('CHEBI:29785', 'Chemical') :: ['NO(2)(-)'] @entity291 :: ('9913', 'Species') :: ['bovine', 'Bovine'] @entity2832 :: ('MESH:C066906', 'Chemical') :: ['SDM']
@entity291 :: (9913,Species) :: ['bovine']
