UNASSIGNED: Heparin has shown promise as a component of various biomaterial formulations, but its variable properties and inhibitory effects on some cell types have raised interest in use of @entity13237 as an alternative. In this study, we characterized the interactions of vascular @entity3960 ( @entity3960 ) and endothelial cells (EC) with heparin and @entity13237 immobilized onto chitosan-based films. Films were modified by blending chitosan with type I collagen and covalently attaching heparin or @entity13237 at various levels. Cell-material interactions were evaluated by quantifying cell spreading, shape and proliferation rate. ECs proliferated well on chitosan, but the polymer was a mediocre substrate for @entity3960 growth. Immobilizing heparin on chitosan further inhibited @entity3960 proliferation. However, blending collagen reversed the heparin inhibition of @entity3960 growth, resulting in a pro-proliferative effect of heparin immobilized on chitosan-collagen films. @entity13237 surfaces supported both @entity3960 and EC proliferation with or without the presence of collagen. The results indicate that inhibitory effects of heparin on @entity3960 are reversed by immobilization in the presence of collagen, and that @entity13237 may be superior to heparin as a biomaterial additive for promoting vascular cell growth in chitosan-based scaffolds. This article is protected by copyright. All rights reserved.

Morphological and Growth Responses of Vascular Smooth Muscle and Endothelial Cells Cultured on Immobilized Heparin and XXXX Surfaces.

@entity3960 :: ('MESH:D018235', 'Disease') :: ['smooth muscle', 'SMC'] @entity13237 :: ('MESH:D016264', 'Chemical') :: ['dextran sulfate', 'Dextran sulfate']
@entity13237 :: (MESH:D016264,Chemical) :: ['Dextran Sulfate']
