UNASSIGNED: High-risk @entity1082 (HR-HPV) is a major etiologic agent in a subset of @entity213 ( @entity213 ), and its recognition has prognostic and predictive implications. The availability of a sensitive and specific test to assess HR-HPV status is limited. We evaluate an RNA in situ hybridization (ISH) method using branch chain technology to detect HR-HPV and compare its results with DNA ISH, @entity986 immunohistochemistry, and polymerase chain reaction (PCR). Tissue sections from 54 @entity1 were stained with a manual RNA ISH assay (ViewRNA), which detects 14 HR-HPV types, an automated DNA ISH assay, and @entity986 immunohistochemistry. Most cases (83%, n=45) were also tested on an automated platform for 14 @entity8811 limited to @entity1799 /18. PCR was performed in all cases and was successful in 93% (n=50). The RNA ISH assay produced results in 96% of the cases with strong signals and was easily interpreted. HR-HPV was detected in more cases (63%, n=34) by RNA ISH than by DNA ISH (39%, n=21). Compared with PCR, both ISH platforms were 94% specific. RNA ISH was more sensitive (91%) than DNA ISH (65%), and RNA ISH correlated more strongly with @entity986 immunostaining. @entity1799 represented 89% of HR-HPV detected. The cocktail @entity1799 /18 platform was concordant with the pooled HR-HPV assay in all expected cases. The automated assay demonstrated high concordance (96%) with the manual version, showed decreased background, and should allow for easy implementation into the workflow of the diagnostic pathology laboratory.

Performance of a Branch Chain RNA In Situ Hybridization Assay for the Detection of High-risk XXXX in @entity213 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity986 :: ('1029', 'Gene') :: ['p16'] @entity8811 :: ('MESH:D013631', 'Disease') :: ['HR-HPV types and 1'] @entity1799 :: ('333760', 'Species') :: ['HPV 16'] @entity1082 :: ('10566', 'Species') :: ['human papillomavirus'] @entity213 :: ('MESH:C535575', 'Disease') :: ['HNSCCs', 'head and neck squamous cell carcinomas']
@entity1082 :: (10566,Species) :: ['Human Papillomavirus']
