AIM: To investigate the expression pattern of @entity1656 signaling in odontoblast-like cells stimulated by lipopolysaccharide (LPS) in vitro, and in injured @entity35 dental pulp in vivo. METHODOLOGY: @entity19 odontoblast-like cells (MDPC-23) were exposed to LPS. Expression of @entity1656 -related genes was detected by Real-time PCR. A @entity35 @entity11624 model was established by @entity130 and LPS plus @entity130 followed by analysis of expression of @entity24805 by immunohistochemical staining. One-way analysis of variance (ANOVA) was performed to examine the effect of differing concentrations of LPS on cell proliferation, and least significant difference (LSD) test was used for paired comparisons. For independent sample, t-test was performed to compare the expression of @entity1656 signaling genes between LPS group and control group in vitro. RESULTS: The in vitro study revealed the proliferation of MDPC-23 cells on exposure to 10 ng mL(-1) to 1  g mL(-1) LPS. Expression of @entity1656 , 2 was significantly higher in the LPS group than that in the control group on day 1 and day 3 (P  0.05). The levels of both @entity61733 and @entity1657 were higher in the study group than in the control group on day 3 (P=0.019 and P=0.034) and day 5 (P  0.001 and P=0.046), respectively. In addition, @entity49547 levels were significantly higher in the study group than in the control group on day 5 (P=0.005). The in vivo study demonstrated positive staining for @entity24805 , both in the @entity130 ( @entity130 ) group and in the LPS plus @entity130 (LMI) group from day 3 to day 7, which showed very weak or absent staining on day 14, thereby demonstrating the dynamic nature of the change. CONCLUSIONS: Both in vitro and in vivo activation and dynamic expression of @entity1656 signaling in dental pulp cells after injury were found. @entity1656 signaling activation by LPS stimulation or @entity130 showed a similar pattern in vivo. This article is protected by copyright. All rights reserved.

Activation and dynamic expression of XXXX signaling in dental pulp cells after injury in vitro and in vivo.

@entity19 :: ('10090', 'Species') :: ['Mouse'] @entity1657 :: ('29146(Tax:10116)', 'Gene') :: ['Jagged1'] @entity49547 :: ('29577(Tax:10116)', 'Gene') :: ['Hes1'] @entity1656 :: ('25496(Tax:10116)', 'Gene') :: ['Notch 1', 'Notch'] @entity11624 :: ('MESH:D011671', 'Disease') :: ['pulpitis'] @entity61733 :: ('84010(Tax:10116)', 'Gene') :: ['Delta1'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity24805 :: ('29492(Tax:10116)', 'Gene') :: ['Notch2'] @entity130 :: ('MESH:D014947', 'Disease') :: ['mechanical injury', 'MI']
@entity1656 :: (25496(Tax:10116),Gene) :: ['Notch']
