There is now strong evidence that the ultraviolet A (UVA) part of the solar spectrum contributes to the development of @entity1489 . Its effect on the skin immune system, however, has not been fully investigated. Here, we analyzed the effects of UVA radiation on dermal dendritic cells (DDC), which, in addition, provided further characterization of these cells. Dermal sheets were obtained from normal @entity1 skin and irradiated, or not, with UVA at 2 or 12 J per cm2. After a 2 d incubation, the phenotype of emigrant cells was analyzed by double immunostaining and flow cytometry. Results showed that migratory DDC were best characterized by @entity4897 expression and that only few cells co-expressed the Langerhans cell marker @entity9184 . Whereas the DC extracted from the dermis displayed an @entity44 phenotype, emigrant DDC showed increased expression of HLA-DR and acquired co-stimulation and maturation markers. We showed here that UVA significantly decreased the number of viable emigrant DDC, a process related to increased apoptosis. Furthermore, UVA irradiation impaired the phenotypic and functional maturation of migrating @entity7725 into potent antigen-presenting cells, in a concentration-dependent manner. The results provide further evidence that UVA are immunosuppressive and suggest an additional mechanism by which solar radiation impairs immune response.

UVA radiation impairs phenotypic and functional maturation of XXXX dermal dendritic cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity9184 :: ('50489', 'Gene') :: ['Langerin'] @entity1489 :: ('MESH:D012878', 'Disease') :: ['skin cancers'] @entity4897 :: ('911', 'Gene') :: ['CD1c'] @entity7725 :: ('CHEBI:10101', 'Chemical') :: ['DDC'] @entity44 :: ('MESH:D013724', 'Disease') :: ['immature']
@entity1 :: (9606,Species) :: ['human']
