UNASSIGNED: Although local cell delivery is an option to repair tissues, particularly using chitosan-based hydrogels, significant attrition of injected cells prior to engraftment has been a problem. To address this problem, we explored the possibility of stabilizing the chitosan-gelatin (CG) injectable hydrogels using (1) controlled release of @entity3403 ( @entity3105 ) to prevent premature degradation due to increased gelatinase activity ( @entity1476 and @entity1495 ), and (2) transglutaminase (TG) to in situ cross-link gelatin to improve the mechanical stability. We prepared @entity3105 -loaded @entity7050 nanoparticles, loaded into the CG hydrogels, measured @entity3105 release for 5  days, and modeled using a single-compartmental assumption. Next, we assessed the influence of TG and @entity3105 on hydrogel compression properties by incubating hydrogels for 7  days in PBS. We evaluated the effect of these changes on retention of fibroblasts and alterations in @entity1476 / @entity1495 activity by seeding 500,000 fibroblasts for 5  days. These results showed that 90  % of @entity3105 released from cross-linked CG hydrogels after 4  days, unlike CG hydrogels where 90  % of @entity3105 was released within the first day. Addition of TG enhanced the CG hydrogel stability significantly. More than 60  % of seeded fibroblasts were recovered from the CG-TG hydrogels at day 5, unlike 40  % recovered from CG-hydrogels. Inhibition of @entity1476 / @entity1495 were observed. In summary, controlled release of @entity3105 from CG hydrogels cross-linked with TG shows a significant potential as a carrier for cell delivery.

Improving the stability of chitosan-gelatin-based hydrogels for cell delivery using transglutaminase and controlled release of XXXX .

@entity1476 :: ('4313', 'Gene') :: ['MMP-2'] @entity3403 :: ('MESH:D004318', 'Chemical') :: ['doxycycline'] @entity7050 :: ('MESH:C043435', 'Chemical') :: ['PLGA'] @entity1495 :: ('4318', 'Gene') :: ['MMP-9'] @entity3105 :: ('D004317', 'Chemical') :: ['DOX']
@entity3403 :: (MESH:D004318,Chemical) :: ['doxycycline']
