We have purified various @entity24635 isoenzymes from roots and hairy-root cultures of turnip ( @entity4335 ) which could potentially be used for commercial applications such as an enzyme immunoassays, diagnostic test kits, wastewater treatment and soil remediation. One of them, a basic @entity24635 called HR2, was secreted into the medium of turnip hairy-root cultures. HR2 had a pI of 9.6, a molecular mass of 39.3 kDa and showed great thermostability. The inactivation of HR2 by @entity274 in the absence of reductant substrates was studied. Under these conditions @entity274 acted as a suicide substrate. The kinetic constants calculated have been compared with those of a basic isoperoxidase from horseradish (Armoracia sp.) roots (HRP-C), which is commonly used in commercial kits. The results for HR2 indicated that it was more resistant to inactivation because it presented a lower inactivation efficiency and a higher value for the partition ratio (r=1250) than those described for HRP-C. These results make turnip @entity24635 HR2 suitable for use in systems in which high @entity274 concentrations are found. Such an application is demonstrated, namely an enzymic diagnostic kit for determination of @entity414 in which HR2 was found to be as efficient as the enzyme originally included in standard kits.

A @entity24635 isoenzyme secreted by turnip ( XXXX ) hairy-root cultures: inactivation by hydrogen peroxide and application in diagnostic kits.

@entity24635 :: ('106444911', 'Gene') :: ['peroxidase'] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H2O2'] @entity4335 :: ('3708', 'Species') :: ['Brassica napus'] @entity414 :: ('MESH:D014527', 'Chemical') :: ['uric acid']
@entity4335 :: (3708,Species) :: ['Brassica napus']
