BACKGROUND: @entity2391 displays anti-inflammatory and immunosuppressive activity and has been considered for the treatment of @entity309 ( @entity309 ). Information about the effects of this molecule on joint cells is scarce, however. OBJECTIVE: To investigate the effects of @entity2391 on the production of @entity1193 ( @entity1193 ) in @entity1 articular chondrocytes and synovial fibroblasts. METHODS: Chondrocytes and synovial fibroblasts were stimulated with @entity2391 alone or in combination with @entity1356 . @entity1193 concentrations in culture supernatants and cell lysates were determined by ELISA. Expression of mRNA encoding the secreted sIL1Ra or the intracellular icIL1Ra1 isoforms was quantified by real time reverse transcriptase-polymerase chain reaction. RESULTS: In chondrocytes, @entity2391 alone had no effect, but dose dependently enhanced the secretion of @entity1193 induced by @entity1356 . Chondrocyte cell lysates contained undetectable or low levels of @entity1193 , even after stimulation with @entity1356 and @entity2391 . Consistently, @entity1356 and @entity2391 induced sIL1Ra mRNA expression in chondrocytes, while expression of icIL1Ra1 was not detectable. @entity1 articular chondrocytes thus mainly produce secreted @entity1193 . In synovial fibroblasts, @entity2391 alone dose dependently increased @entity1193 secretion. In addition, @entity2391 enhanced the stimulatory effect of @entity1356 on @entity1193 production. In synovial cell lysates, @entity2391 and @entity1356 also increased @entity1193 levels. Consistently, @entity2391 and @entity1356 induced the expression of both sIL1Ra and icIL1Ra1 mRNA in synovial fibroblasts. CONCLUSION: @entity2391 increases @entity1193 production in joint cells, which may be beneficial in cartilage damaging diseases such as @entity309 or @entity571 .

Interferon beta stimulates interleukin 1 receptor antagonist production in XXXX articular chondrocytes and synovial fibroblasts.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity571 :: ('MESH:D010003', 'Disease') :: ['osteoarthritis'] @entity1356 :: ('3553', 'Gene') :: ['IL1beta', 'interleukin (IL) 1beta'] @entity1193 :: ('3557', 'Gene') :: ['IL1Ra', 'interleukin-1 receptor antagonist'] @entity2391 :: ('3456', 'Gene') :: ['Interferon (IFN) beta', 'IFNbeta'] @entity309 :: ('MESH:D001172', 'Disease') :: ['rheumatoid arthritis', 'RA']
@entity1 :: (9606,Species) :: ['human']
