It has been previously demonstrated that chemokine monocyte chemoattractant protein-1 ( @entity657 / @entity657 ) increases the excitability of nociceptive neurons after @entity1897 or @entity32 . Moreover, decreased nocifensive mechanical threshold in behavioral tests and increased @entity460 influx in cultured dorsal root ganglion neurons by @entity657 application have been reported. However, the effects of @entity657 on peripheral afferent terminals have not been studied yet. The present study aimed to examine the effect of @entity657 on the response of cutaneous unmyelinated afferents. For this purpose, single fiber recordings of mechanosensitive C-afferents were made in vitro from skin-saphenous nerve preparations excised from @entity35 euthanized by @entity515 . Since IB4-positive neurons were previously implicated in @entity657 induced @entity1931 , sensitivity to a, b-methylene ATP (metATP), an indicator of IB4-positive neurons, was also studied. Application of @entity657 100ng/ml to the receptive field elicited excitation in one half of mechanosensitive unmyelinated afferents in the skin. @entity657 also sensitized metATP insensitive fibers to mechanical stimulation, but not metATP sensitive fibers. The incidence of heat sensitive fibers was decreased in the @entity657 treated group with a decrease in the response threshold. These results demonstrate @entity657 is an effective stimulant of mechanosensitive unmyelinated peripheral afferents in the @entity35 skin.

Monocyte chemoattractant protein-1-induced excitation and sensitization to mechanical stimulation of mechanosensitive C-fiber afferents in XXXX skin.

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity1931 :: ('MESH:D006930', 'Disease') :: ['mechanical hyperalgesia'] @entity657 :: ('24770(Tax:10116)', 'Gene') :: ['CCL2', 'MCP-1'] @entity515 :: ('CHEBI:29334', 'Chemical') :: ['CO2'] @entity1897 :: ('MESH:D010523', 'Disease') :: ['peripheral nerve injury'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats']
@entity35 :: (10116,Species) :: ['rat']
