Beta-adrenergic activation stimulates @entity4305 ( @entity4305 ), enhancing metabolic rate. In vitro, most work has studied brown adipocytes, however, few have investigated more established adipocyte lines such as the @entity19 3T3-L1 line. To assess the effect of beta-adrenergic activation, mature 3T3-L1s were treated for 6 or 48 hours with or without @entity3044 (10 and 100  M) following standard differentiation supplemented with @entity6145 (T3; 1 nM). The highest dose of @entity3044 increased lipid content following 48 hours of treatment. This concentration enhanced @entity4305 mRNA and protein expression. The increase in @entity4305 following 48 hours of @entity3044 increased @entity26 consumption rate. Further, coupling efficiency of the electron transport chain was disturbed and an enhancement of glycolytic rate was measured alongside this, indicating an attempt to meet the energy demands of the cell. Lastly, markers of beige adipocytes (protein content of @entity23918 and gene transcript of @entity61185 ) were also found to be upregulated at 48 hours of @entity3044 treatment. This data indicates that mature 3T3-L1 adipocytes are responsive to @entity3044 and induce @entity4305 expression and activity. Further, this finding provides a model for further pharmaceutical and nutraceutical investigation of @entity4305 in 3T3-L1s.

XXXX Increases Uncoupling, Glycolysis, and Markers of Beiging in Mature 3T3-L1 Adipocytes.

@entity61185 :: ('12705(Tax:10090)', 'Gene') :: ['CITED1'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity4305 :: ('22227(Tax:10090)', 'Gene') :: ['uncoupling protein 1', 'UCP1'] @entity3044 :: ('MESH:D007545', 'Chemical') :: ['isoproterenol'] @entity23918 :: ('21942(Tax:10090)', 'Gene') :: ['CD137'] @entity6145 :: ('CHEBI:60311', 'Chemical') :: ['thyroid hormone']
@entity3044 :: (MESH:D007545,Chemical) :: ['Isoproterenol']
