Tricyclic antidepressant ( @entity2974 ) drugs are also used as adjunctive therapy to treat @entity1118 . To monitor @entity1 compliance to therapy, urine specimens may be preferred since collection is non-invasive, and the specimen can provide a longer detection window. @entity2974 drugs are frequently monitored by immunoassay; however, poor antibody specificity may compromise results. The purpose for this study was to develop a confirmation method for determining @entity2974 in urine specimens by ultra-pressure liquid chromatography-tandem mass spectrometry (UPLC- @entity15 / @entity15 ). Our method can quantify 9 @entity2974 drugs in less than 5 min. Method validation experiments were performed, and the coefficient variation for inter- and intra-day precision was within 12% for each analyte at five different concentrations. Accuracy studies had good agreement with another laboratory that performs testing by GC/ @entity15 . HIGHLIGHTS: 1. Tricyclic antidepressants are commonly used to treat @entity308 , @entity148 , and @entity9 . 2. A confirmation method by ultra-pressure liquid chromatography-tandem mass spectrometry (UPLC- @entity15 / @entity15 ) to determine @entity2974 in urine specimens was developed. 3. Urine testing for @entity2974 is preferred for monitoring adherence to therapy due to ease of collection.

Method Validation of a Tricyclic Antidepressant Drug Panel in Urine by UPLC- XXXX / @entity15 .

@entity1 :: ('9606', 'Species') :: ['patient'] @entity308 :: ('MESH:D003866', 'Disease') :: ['depression'] @entity2974 :: ('CHEBI:30956', 'Chemical') :: ['TCA'] @entity148 :: ('MESH:D001008', 'Disease') :: ['anxiety'] @entity15 :: ('MESH:D009103', 'Disease') :: ['MS'] @entity1118 :: ('MESH:D059350', 'Disease') :: ['chronic pain'] @entity9 :: ('MESH:D009437', 'Disease') :: ['neuropathic pain']
@entity15 :: (MESH:D009103,Disease) :: ['MS']
