UNASSIGNED: Bone marrow mesenchymal stromal cells (BM-MSCs) are multipotent cells capable of differentiating toward osteoblatic and adipocytic phenotypes. BM-MSCs play several key roles including bone remodeling, establishment of @entity1583 and immune tolerance induction. Here, we investigated the effect of resveratrol ( @entity5842 ), a therapeutically promising natural polyphenol, on the commitment of @entity1 BM-MSCs primary cultures. Cell differentiation was evaluated by means of morphological analysis, specific staining and expression of osteogenic and adipocytic master genes ( @entity8087 , @entity6864 ). To maintain BM-MSC multipotency, all experiments were performed on cells at very early passages. At any concentration @entity5842 , added to standard medium, did not affect the phenotype of confluent BM-MSCs, while, when added to osteogenic or adipogenic medium, 1 M @entity5842 enhances the differentiation toward osteoblasts or adipocytes, respectively. Conversely, the addition of higher @entity5842 concentration (25 M) to both differentiation media resulted exclusively in BM-MSCs adipogenesis. Surprisingly, the analysis of @entity5842 molecular effects demonstrated that the compound completely substitutes insulin, a key component of adipogenic medium. We also observed that @entity5842 treatment is associated to enhanced phosphorylation of CREB, a critical effector of insulin adipogenic activity. Finally, our observations contribute to the mechanistic elucidation of the well-known @entity5842 positive effect on insulin sensitivity and @entity6 .

Resveratrol mimics insulin activity in the adipogenic commitment of XXXX bone marrow mesenchymal stromal cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity5842 :: ('11886', 'Species') :: ['RSV'] @entity8087 :: ('860', 'Gene') :: ['Runx-2'] @entity6 :: ('MESH:D003920', 'Disease') :: ['type 2 diabetes mellitus'] @entity1583 :: ('MESH:D019337', 'Disease') :: ['hematopoietic niche'] @entity6864 :: ('5468', 'Gene') :: ['PPARy']
@entity1 :: (9606,Species) :: ['human']
