UNASSIGNED: The transcription factor NF-kB mediates inflammation and stress signals in cells. To test NF-kB in the control of @entity99 , we inactivated NF-kB in liver of C57BL/6 @entity19 through inactivation of @entity1992 gene, which was achieved by crossing floxed @entity1992 and @entity14415 -cre @entity19 to generate L- @entity1992 -KO @entity19 . KO @entity19 did not exhibit any alterations in growth, reproduction and body weight on a Chow diet. However, the @entity19 exhibited an improvement in @entity1720 on a high fat diet (HFD). @entity99 was enhanced in the @entity19 as indicated by increased @entity2388 tolerance, Akt phosphorylation and decreased gene expression in hepatic gluconeogenesis. In the liver, a decrease in intracellular @entity268 was observed with decreased @entity15983 phosphorylation. Cyclic @entity4334 phosphodiesterase 3B ( @entity45044 ), a @entity268 degrading enzyme, was increased in mRNA and protein. The increase was a result of absence of NF-kB activity. NF-kB was found to inhibit @entity45044 transcription through three DNA-binding sites in the gene promoter in response to @entity637 . Body composition, food intake, energy expenditure, @entity99 were not significantly altered in KO @entity19 on HFD. These data suggest that NF-kB may inhibit @entity99 by up-regulating @entity268 through suppression of @entity45044 gene transcription.

Inactivation of NF-kB @entity1992 ( @entity1992 ) in liver improved insulin sensitivity and inhibited XXXX /PKA pathway.

@entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity1992 :: ('19697(Tax:10090)', 'Gene') :: ['p65'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity14415 :: ('11657(Tax:10090)', 'Gene') :: ['Alb'] @entity15983 :: ('12912(Tax:10090)', 'Gene') :: ['CREB'] @entity45044 :: ('18576(Tax:10090)', 'Gene') :: ['PDE3B'] @entity4334 :: ('D009711', 'Chemical') :: ['nucleotide'] @entity1720 :: ('MESH:D007333', 'Disease') :: ['systemic insulin sensitivity'] @entity99 :: ('MESH:D056486', 'Disease') :: ['Hepatic insulin sensitivity', 'systemic and hepatic inflammation', 'hepatic insulin sensitivity'] @entity637 :: ('21926(Tax:10090)', 'Gene') :: ['TNF-a'] @entity2388 :: ('MESH:D019289', 'Chemical') :: ['pyruvate']
@entity268 :: (CHEBI:17489,Chemical) :: ['cAMP']
