BACKGROUND: The cell line HaCaT/SM was derived from the @entity1 keratinocyte cell line HaCaT. HaCaT/SM cells display a high resistance against @entity599 mustard (SM). Intention of the presented study was to determine the cellular and molecular differences between HaCaT/SM and HaCaT so as to evaluate which changes might be responsible for being resistant against SM. METHODS: Both cell lines HaCaT and HaCaT/SM were analyzed with respect to their cell growth, nuclei perimeter, clonogenicity and secretion profile. Moreover DNA alkylation pattern under presence of SM was investigated. RESULTS: In comparison to HaCaT, the HaCaT/SM showed a significant smaller nuclei perimeter. For DNA alkylation a significant difference was observed over time. The clonogenicity of HaCaT/SM was increased to 150%. The secretion profile of these cells demonstrated a strong increase of @entity7741 , PDGF-AA, @entity2843 , @entity1552 , and a decrease of @entity1915 , @entity5737 , CXC1, @entity17184 , @entity25296 , @entity4938 , @entity687 , @entity371 , @entity6785 , Trappin-1. CONCLUSION: The @entity599 mustard (SM) resistant cell line HaCaT/SM demonstrates a wide range of significant differences to their origin cell line HaCaT. These differences might be responsible to provide resistance against SM and might also be useful to establish treatment concepts for @entity1 after SM exposure.

Characterization of XXXX mustard resistant keratinocyte cell line HaCaT/SM.

@entity1 :: ('9606', 'Species') :: ['human', 'humans'] @entity1552 :: ('7077', 'Gene') :: ['TIMP2'] @entity1915 :: ('374', 'Gene') :: ['AREG'] @entity5737 :: ('6352', 'Gene') :: ['CCL5'] @entity6785 :: ('4282', 'Gene') :: ['MIF'] @entity687 :: ('3576', 'Gene') :: ['CXCL8'] @entity2843 :: ('7076', 'Gene') :: ['TIMP1'] @entity25296 :: ('6372', 'Gene') :: ['CXCL6'] @entity17184 :: ('6376', 'Gene') :: ['CXC2/3'] @entity371 :: ('3627', 'Gene') :: ['CXCL10'] @entity599 :: ('MESH:D013455', 'Chemical') :: ['sulfur'] @entity7741 :: ('283', 'Gene') :: ['ANG'] @entity4938 :: ('5473', 'Gene') :: ['CXCL7']
@entity599 :: (MESH:D013455,Chemical) :: ['sulfur']
