UNASSIGNED: A new method of fabricating all- @entity852 devices with integrated electrodes and fluidic tubing is described. As opposed to expensive @entity852 (PS) fabrication techniques that use hot embossing and bonding with a heated lab press, this approach involves solvent-based etching of channels and lamination-based bonding of a PS cover, all of which do not need to occur in a clean room. PS has been studied as an alternative microchip substrate to @entity16549 , as it is more hydrophilic, biologically compatible in terms of cell adhesion, and less prone to absorption of hydrophobic molecules. The etching/lamination-based method described here results in a variety of all-PS devices, with or without electrodes and tubing. To characterize the devices, micrographs of etched channels (straight and intersected channels) were taken using confocal and scanning electron microscopy. Microchip-based electrophoresis with repetitive injections of @entity941 was conducted using a three-sided PS (etched pinched, twin-tee channel) and one-sided PDMS device. Microchip-based flow injection analysis, with @entity935 and NO as analytes, was used to characterize the performance of all-PS devices with embedded tubing and electrodes. Limits of detection for @entity935 and NO were 130 nM and 1.8  M, respectively. Cell immobilization studies were also conducted to assess all-PS devices for cellular analysis. This paper demonstrates that these easy to fabricate devices can be attractive alternative to other PS fabrication methods for a wide variety of analytical and cell culture applications.

Fabrication and Characterization of All- XXXX Microfluidic Devices with Integrated Electrodes and Tubing.

@entity935 :: ('MESH:D004298', 'Chemical') :: ['dopamine'] @entity852 :: ('MESH:D011137', 'Chemical') :: ['polystyrene'] @entity16549 :: ('CHEBI:61468', 'Chemical') :: ['PDMS'] @entity941 :: ('MESH:D019793', 'Chemical') :: ['fluorescein']
@entity852 :: (MESH:D011137,Chemical) :: ['Polystyrene']
