Transgenic @entity286 ( @entity286 .) plants ubiquitously accumulating a single-chain variable-fragment (scFv) antibody against @entity2271 ( @entity2272 ) to high concentrations in the endoplasmic reticulum (RA plants) show a wilty phenotype. High stomatal conductance and loss of @entity515 and light dependence of stomatal conductance are typical features of these plants. @entity2272 was applied to these plants either via the petioles or by daily spraying over several weeks in order to normalise the phenotype. During the long-term experiments, scFv protein concentrations, total and (calculated) free @entity2272 contents, and stomatal conductance and its dependence on @entity515 concentration and light intensity were monitored. The wilty phenotype of transgenic plants could not be normalised by short-term treatment with @entity2272 via the petioles. Only a daily long-term treatment during plant development normalised the physiological behaviour completely. Scanning electron microscopy of stomata showed morphological changes in RA plants compared with wild-type plants that, for structural reasons, prevented regular stomatal movements. After long-term treatment with @entity2272 this defect could be completely eliminated. Guard-cell-specific expression of the anti- @entity2272 scFv did not cause any changes in physiological behaviour compared to the wild type. In addition, mesophyll-specific expression starting in leaves that were already fully differentiated resulted in normal phenotypes, too. We conclude that changes in distribution and availability of @entity2272 in the cells of developing leaves of RA plants cause the development of structural features in stomata that prevent normal function.

Prevention of stomatal closure by immunomodulation of endogenous @entity2271 and its reversion by XXXX treatment: physiological behaviour and morphological features of @entity286 stomata.

@entity2272 :: ('CHEBI:2365', 'Chemical') :: ['ABA'] @entity286 :: ('4097', 'Species') :: ['Nicotiana tabacum L', 'tobacco'] @entity2271 :: ('MESH:D000040', 'Chemical') :: ['abscisic acid'] @entity515 :: ('CHEBI:29334', 'Chemical') :: ['CO(2)']
@entity2271 :: (MESH:D000040,Chemical) :: ['abscisic acid']
