BACKGROUND: Tissue transglutaminase enzyme-linked immunosorbent assay (tTG-ELISA) has recently been proposed as a simple and fast screening test for @entity1593 ( @entity1593 ). The rate of false-positive and false-negative tests with tTG-ELISA, however, has not been definitively established. Therefore, the aim of our study was to investigate anti-tTG antibodies (TGA) not only in untreated @entity1 with @entity1593 and in healthy controls, but also in a large group of @entity1 with other @entity792 . METHODS: The presence of TGA was investigated in sera from 111 @entity1 with untreated @entity1593 , 96 @entity1 with other autoimmune conditions (28 with @entity409 , 46 with @entity1723 , 10 with @entity29 , 12 with @entity12010 ) and from 100 healthy controls using @entity840 tTG-ELISA (gp-TG/ELISA) and highly purified recombinant @entity1 tTG-ELISA (h-TG/ELISA). Western blotting with @entity840 tTG was also performed. RESULTS: Ninety-four @entity1 with @entity1593 who tested positive for antiendomysial antibodies (AEA) and one who tested negative for AEA showed antibodies against the gp-TG. Among the controls, 50% of @entity1 with @entity409 and 6.5% of @entity1 with @entity1723 tested positive with gp-TG/ELISA. Western blotting experiments revealed that the high rate of positive tests observed using ELISA among the control group sera is attributable to impurities in the gp-TG preparation. However, h-TG/ELISA tests were positive for the sera from all @entity1 who tested positive for AEA and from one control who tested negative for AEA, whereas h-TG/ELISA tests were negative for all @entity1593 @entity1 who tested negative for AEA and for other controls who tested negative for AEA. CONCLUSIONS: The frequency of false-negative and false-positive tests represents the major limit to the use of gp-tTG/ELISA. However, because h-TG/ELISA is both simple and fast, it could be used in large screening programs for @entity1593 .

Antitissue transglutaminase antibodies outside XXXX .

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity1593 :: ('MESH:D002446', 'Disease') :: ['celiac disease', 'CD'] @entity840 :: ('10141', 'Species') :: ['guinea pig'] @entity409 :: ('MESH:D008107', 'Disease') :: ['autoimmune liver disease'] @entity29 :: ('MESH:D015212', 'Disease') :: ['inflammatory bowel syndrome'] @entity1723 :: ('MESH:D003922', 'Disease') :: ['insulin-dependent diabetes mellitus'] @entity12010 :: ('MESH:D016884', 'Disease') :: ['type 1 polyglandular syndrome'] @entity792 :: ('MESH:D001327', 'Disease') :: ['autoimmune diseases']
@entity1593 :: (MESH:D002446,Disease) :: ['celiac disease']
