OBJECTIVE: To investigate the expression of chemokine @entity1570 ( @entity436 @entity436 in @entity1 gingival mesenchymal stem cells (GMSCs) and the migration potential of GMSCs stimulated with @entity1570 . METHODS: @entity1 GMSCs were isolated by single-cell cloning method. Their cell surface markers were characterized by flow cytometry, and the rate of colony formation was evaluated. Differentiation assay was used to detect the differentiation potential of GMSCs. The expression of chemokine SDF-l receptor @entity436 in GMSCs was detected by immunocytochemical staining. The chemotactic effect of @entity1570 on GMSCs was detected using a 24-multiwell Transwell cell culture chamber. The number of net migrated cells was counted in different microscope fields. RESULTS: @entity1 GMSCs possessed high self-renewal potential and formed single-cell colonies cultured in vitro. GMSCs expressed mesenchymal stem cells-associated markers @entity1759 , @entity12359 , @entity5399 , CD105, and @entity2394 , and the expression of hemopoietic stem cell surface markers @entity5750 , @entity2280 , and @entity1195 was negative. GMSCs differentiated into osteoblasts and adipocytes under defined culture conditions. The colony forming unit-fibroblastic for GMSCs was 21.4%/  2.8%. Immunocytochemical staining demonstrated that GMSCs expressed chemokine @entity436 @entity436 . The number of GMSCs migrating at concentrations of 100 ng.mL-1 and 200 ng.mL-1 of SDF-l in the Transwell cell culture chamber was significantly higher than that of the negative control (189.3  4.4, 164.6  4.9 cells/field vs. 47.8  2.5 cells/field, P<0.01). Treatment with the @entity436 neutralizing antibody, an antagonist for @entity436 , significantly reduced the migratory effect compared with the negative controls (29.0  2.4 cells/field vs. 47.8  2.5 cells/field, P<0.01). CONCLUSION: @entity1 GMSCs express chemokine SDF-l receptor @entity436 . @entity1570 may participate in regulating chemotaxis of @entity1 GMSCs. Results suggest that the migration induced by @entity1570 is mediated by @entity436 .

[Culturing and characterization of XXXX gingival mesenchymal stem cells and their chemotactic responses to @entity1570 ].

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity5750 :: ('929', 'Gene') :: ['CD14'] @entity1570 :: ('6387', 'Gene') :: ['stromal cell-derived factor-1', 'SDF-1'] @entity1195 :: ('5788', 'Gene') :: ['CD45'] @entity1759 :: ('960', 'Gene') :: ['CD44'] @entity5399 :: ('7070', 'Gene') :: ['CD90'] @entity12359 :: ('4907', 'Gene') :: ['CD73'] @entity2280 :: ('947', 'Gene') :: ['CD34'] @entity2394 :: ('214', 'Gene') :: ['CD166'] @entity436 :: ('7852', 'Gene') :: ['SDF-1 receptor', 'CXCR4', 'SDF-1) receptor']
@entity1 :: (9606,Species) :: ['human']
