The ability of the testis to convert irreversibly @entity3800 into @entity172 is related to the presence of a microsomal enzymatic complex named aromatase. In rodents, germ cell production of @entity172 is known, although the regulation of the cytochrome p450 aromatase ( @entity18946 ) gene expression is not completely elucidated. In the present study, we have investigated the putative effects of @entity230 ( @entity251 , @entity586 ( @entity587 ) and @entity53 ) on @entity18946 gene expression in purified adult @entity35 pachytene spermatocytes (PSs) and round spermatids (RSs). Using a highly specific quantitative competitive RT-PCR method we established that @entity251 enhances in a dose- and time-related manner aromatase gene expression in PSs and RSs; @entity586 induces the same effect. Furthermore, @entity251 increases the @entity53 output in both germ cell populations whereas 5alpha- @entity587 was inefficient, therefore suggesting that the effect of @entity3800 on @entity18946 gene transcription was independent of @entity172 formation. In fact @entity53 inhibits the @entity18946 gene expression in PSs and RSs. @entity1299 , an @entity172 receptor antagonist, has no effect on @entity251 -stimulated aromatase expression in PSs and RSs. By contrast, @entity1299 suppresses the inhibitory effect of @entity53 on @entity18946 mRNA expression in PSs and RSs. Similarly, @entity12193 , a non-steroidal anti- @entity342 specific for @entity342 receptors, abolishes the @entity251 -stimulated aromatase expression in PSs and RSs. These observations show that @entity3800 up-regulate aromatase gene expression in purified adult @entity35 germ cells whereas @entity172 exert an opposite effect, which may suggest the presence of @entity342 and @entity172 responsive elements on the aromatase promoter(s).

XXXX control the aromatase gene expression in purified germ cells from the adult male @entity35 .

@entity172 :: ('MESH:D004967', 'Chemical') :: ['estrogen', 'estrogens'] @entity251 :: ('MESH:D013739', 'Chemical') :: ['testosterone'] @entity586 :: ('MESH:D013196', 'Chemical') :: ['5alpha-DHT', '5alpha-dihydrotestosterone'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity53 :: ('MESH:D004958', 'Chemical') :: ['estradiol'] @entity12193 :: ('MESH:C021277', 'Chemical') :: ['nilutamide'] @entity342 :: ('CHEBI:50113', 'Chemical') :: ['androgen'] @entity230 :: ('MESH:D013256', 'Chemical') :: ['steroids'] @entity587 :: ('CHEBI:16330', 'Chemical') :: ['DHT'] @entity1299 :: ('MESH:C070081', 'Chemical') :: ['ICI 182780'] @entity18946 :: ('25147(Tax:10116)', 'Gene') :: ['Cyp19', 'p450 arom'] @entity3800 :: ('MESH:D000728', 'Chemical') :: ['androgens']
@entity230 :: (MESH:D013256,Chemical) :: ['Steroids']
