The @entity1 tissue kallikrein family (KLK for protein; KLK for gene) includes 15 members. Twelve kallikreins, including @entity22546 , are concurrently upregulated in @entity43 . However, the mechanism of this phenomenon remains unclear. In this study, we measured @entity22546 expression in a large series of ovarian tissue cytosols and examined possible mechanisms of @entity22546 up-regulation in @entity43 . Using a newly developed enzyme-linked immunosorbent assay (ELISA) with two monoclonal antibodies, we quantified @entity22546 expression in ovarian tissue cytosols, and confirmed the upregulation of @entity22546 in @entity43 and its unfavourable prognostic value. We then examined @entity22546 mRNA expression using reverse transcription-polymerase chain reaction and established its good concordance with @entity22546 protein expression. This finding suggested that the @entity22546 gene is under transcriptional regulation. We then scrutinised a few mechanisms that could explain @entity22546 upregulation. The relative abundance of two @entity22546 mRNA transcripts was studied; we found the same differential expression pattern in all samples, regardless of @entity22546 levels. Genomic mutation screening of all exons and the 5'-flanking region of the @entity22546 gene identified two linked single- @entity377 polymorphisms in the 5'-untranslated region, but neither correlated with @entity22546 expression. Ovarian cell lines were separately treated with five @entity134 hormones. None of the treatments produced significant effects on @entity22546 expression. We conclude that @entity22546 is transcriptionally upregulated in @entity43 , but probably not through alternative mRNA transcript expression, genomic mutation, or @entity134 hormone induction.

Transcriptional upregulation of @entity1 tissue kallikrein 6 in XXXX : clinical and mechanistic aspects.

@entity1 :: ('9606', 'Species') :: ['human'] @entity43 :: ('MESH:D010051', 'Disease') :: ['ovarian cancer'] @entity22546 :: ('5653', 'Gene') :: ['KLK6'] @entity377 :: ('CHEBI:36976', 'Chemical') :: ['nucleotide'] @entity134 :: ('CHEBI:35341', 'Chemical') :: ['steroid']
@entity43 :: (MESH:D010051,Disease) :: ['ovarian cancer']
