The complete @entity377 sequence of the linear DNA plasmid (pRS224-1) from the plant-pathogenic fungus Rhizoctonia solani isolate H-16 was determined; and its unique RNA transcripts were characterized. The pRS224-1 DNA consists of 4,986 @entity2036 . A computer-based study of the folding of pRS224-1 at both termini predicted hairpin-loop structures. The hairpin loops consisted of the left and right termini of 236 and 264 @entity2036 , respectively, and share no sequence homology. Unique poly(A) RNAs, 4.7 kb and 7.4 kb in length and hybridizing with the pRS224 DNA, were found in mycelial cells of R. solani H-16. Transcript product-mapping allowed the prediction of the locations of different expression signals. The 7.4-kb transcript is generated from the left terminal region of the complementary strand, via the full-length sense-strand, to the right terminal region of the complementary strand. The 4.7-kb transcript is generated from the center region of the sense strand to the right terminal region of the complementary strand. One @entity5545 ( @entity5545 ) found in pRS224-1 is 887 @entity1522 long and has a potential coding capacity of 102 kDa. The @entity5545 contains the highly conserved domains characteristic of reverse transcriptase sequences, including the highly conserved YXDD sequence.

Complete XXXX sequence of the linear DNA plasmid pRS224 with hairpin loops from Rhizoctonia solani and its unique transcriptional form.

@entity5545 :: ('MESH:D004410', 'Disease') :: ['ORF', 'open reading frame'] @entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity377 :: ('CHEBI:36976', 'Chemical') :: ['nucleotide'] @entity2036 :: ('MESH:D009711', 'Chemical') :: ['nucleotides']
@entity377 :: (CHEBI:36976,Chemical) :: ['nucleotide']
