UNASSIGNED: Platelet-rich plasma ( @entity21652 ), which contains highly concentrated platelets, is produced by centrifuging whole blood. It is a safe and readily available source of a wide range of growth factors necessary for angiogenesis. Gelatin hydrogel granules have been designed and prepared for the controlled release of many growth factors. The angiogenic effect of @entity1 @entity21652 was examined in vitro, and the effect of its subcutaneous injection with gelatin hydrogel granules into @entity19 subcutis was evaluated. @entity1 @entity21652 was prepared using a double-spin method. The concentration of growth factors and the platelet count were examined in @entity21652 and in vitro, and the angiogenic activity of @entity1 umbilical vein endothelial cells (HUVECs) in co-culture with @entity1 dermal fibroblast cells (NHDFs) in the presence and absence of @entity21652 was evaluated. Then, in vivo, @entity21652 , either free or with gelatin hydrogel granules, was injected subcutaneously into tiebacks on @entity19 . Using a microscope and Kurabo angiogenesis image analyser software, the area containing newly formed capillaries was evaluated histologically and the microvascular network score was calculated. @entity21652 was shown to contain high concentrations of PDGF, @entity1611 and @entity641 and had an angiogenic effect on the co-culture system. @entity21652 with gelatin hydrogel granules significantly enlarged the area containing newly formed capillaries and promoted the microvascular network in @entity19 subcutaneous tissue. @entity21652 encapsulated in gelatin hydrogel microspheres shows promise for enhancing angiogenic effects in @entity19 subcutis and could represent a potential therapeutic combination for the treatment of @entity74 . Copyright    2015 John Wiley _ Sons, Ltd.

Angiogenic effect of platelet-rich plasma combined with gelatin hydrogel granules injected into XXXX subcutis.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity641 :: ('21803(Tax:10090)', 'Gene') :: ['TGFb'] @entity19 :: ('10090', 'Species') :: ['mice', 'murine'] @entity21652 :: ('19131(Tax:10090)', 'Gene') :: ['PRP'] @entity74 :: ('MESH:D030342', 'Disease') :: ['ischaemic disorders'] @entity1611 :: ('22339(Tax:10090)', 'Gene') :: ['VEGF']
@entity19 :: (10090,Species) :: ['murine']
