Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in plant innate immunity. Overexpression of StMEK1( @entity336 ), a constitutively active MAPK kinase that activates @entity3785 -induced protein kinase (SIPK) and wound-induced protein kinase (WIPK), provokes @entity66 in Nicotiana benthamiana. Here we purified a 51-kD MAPK, which was activated in @entity415 ( @entity415 ) tubers treated with hyphal wall elicitor of a plant pathogen, and isolated the cDNA designated @entity69070 . The deduced @entity535 sequence of the @entity69070 showed strong similarity to stress-responsive MAPKs, such as @entity286 ( @entity286 ) SIPK and @entity958 ( @entity958 ) @entity32689 . To investigate the downstream signaling of @entity69070 , we identified several proteins phosphorylated by @entity69070 (PPSs) using an in vitro expression cloning method. To dissect the biological function of PPSs in the plant defense, we employed virus-induced gene silencing (VIGS) in N. benthamiana. VIGS of NbPPS3 significantly delayed cell @entity204 induced by the transient expression of StMEK1( @entity336 ) and treatment with hyphal wall elicitor. Furthermore, the mobility shift of NbPPS3 on @entity214 - @entity2975 gel was induced by transient expression of StMEK1( @entity336 ). The mobility shift of NbPPS3 induced by StMEK1( @entity336 ) was not compromised by VIGS of WIPK or SIPK alone, but drastically reduced by the silencing of both WIPK and SIPK. This work strongly supports the idea that PPS3 is a physiological substrate of @entity69070 and is involved in cell @entity204 activated by a MAPK cascade.

Involvement of PPS3 phosphorylated by elicitor-responsive mitogen-activated protein kinases in the regulation of plant cell XXXX .

@entity286 :: ('4097', 'Species') :: ['tobacco', 'Nicotiana tabacum'] @entity3785 :: ('MESH:D020156', 'Chemical') :: ['salicylic acid'] @entity336 :: ('MESH:C536170', 'Disease') :: ['DD'] @entity66 :: ('MESH:D004194', 'Disease') :: ['hypersensitive response-like cell death'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity958 :: ('3702', 'Species') :: ['Arabidopsis thaliana', 'Arabidopsis'] @entity415 :: ('4113', 'Species') :: ['potato', 'Solanum tuberosum'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity32689 :: ('818982(Tax:3702)', 'Gene') :: ['AtMPK6'] @entity69070 :: ('102599364', 'Gene') :: ['StMPK1'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS']
@entity204 :: (MESH:D003643,Disease) :: ['death']
