Because gut-derived factors carried in mesenteric lymph are implicated in multiple @entity881 and have been shown to injure endothelial cells, we investigated several cellular pathways by which this process could occur. To accomplish this, mesenteric lymph (5%, v/v) collected at 1 to 3 h postshock from male @entity35 undergoing @entity130 (5-cm laparotomy) and @entity548 (90 min of mean arterial pressure [MAP] of 30 mmHg; T/HS) was tested for endothelial cell @entity137 on @entity1 umbilical vein endothelial cells (HUVECs). Over 30 pharmacologic agents that had been reported to inhibit endothelial cell @entity204 were tested for their ability to prevent T/HS lymph-induced HUVEC cell @entity204 . These included agents documented to protect against oxidant-mediated, calcium-mediated, and arachidonic acid pathway-mediated @entity130 and @entity204 . These pharmacologic inhibitors were preincubated with HUVECs for 1 h or were added to the HUVECs simultaneously with lymph, and were then incubated for 18 h. Controls were lymph alone, inhibitor alone, or medium alone. Mitochondrial tetrazolium (MTT) and LDH release assays were used to determine cell viability. The inhibitors that significantly protected HUVECs from the @entity137 of T/HS lymph (P < 0.001) included the antioxidant combination of vitamins C and E and the antioxidant-lipooxygenase inhibitor nordihydroguaretic acid (NDGA). These agents were equally effective when added simultaneously with lymph or preincubated with the HUVECs, suggesting an extracellular or membrane-bound process. In summary, the inhibitors that provided protection from toxic lymph appear to work at the membrane and are involved in limiting membrane peroxidation. Based on this study, it appears that an oxidant pathway is involved in T/HS lymph-induced @entity130 and @entity204 .

The role of oxidant-mediated pathways in the XXXX of endothelial cells exposed to mesenteric lymph from @entity35 subjected to @entity548 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity548 :: ('MESH:D006470', 'Disease') :: ['hemorrhagic shock'] @entity881 :: ('MESH:D009102', 'Disease') :: ['organ dysfunction syndrome'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death'] @entity130 :: ('MESH:D014947', 'Disease') :: ['trauma', 'endothelial cell injury']
@entity137 :: (MESH:D064420,Disease) :: ['cytotoxicity']
