The @entity994 protein, a cyclin-dependent kinase (CDK) inhibitor, is capable of binding to both cyclin-CDK and the @entity1364 ( @entity1364 ). Through its binding to @entity1364 , @entity994 can regulate the function of @entity1364 differentially in replication and repair. To gain an understanding of the precise mechanism by which @entity994 affects @entity1364 function, we have designed a new assay for replication factor C (RFC)-catalyzed loading of @entity1364 onto DNA, a method that utilizes a primer-template DNA attached to agarose beads via @entity1885 -streptavidin. Using this assay, we showed that RFC remains transiently associated with @entity1364 on the DNA after the loading reaction. Addition of @entity994 did not inhibit RFC-dependent @entity1364 loading; rather, @entity994 formed a stable complex with @entity1364 on the DNA. In contrast, the formation of a @entity994 - @entity1364 complex on the DNA resulted in the displacement of RFC from the DNA. The nonhydrolyzable analogs of @entity855 , @entity12865 ( @entity12865 ) and @entity17723 , each stabilized the primer recognition complex containing RFC and @entity1364 in the absence of @entity994 . RFC in the ATPgammaS-activated complex was no longer displaced from the DNA by @entity994 . We propose that @entity994 stimulates the dissociation of the RFC from the @entity1364 -DNA complex in a process that requires @entity855 hydrolysis and then inhibits subsequent @entity1364 -dependent events in DNA replication. The data suggest that the conformation of RFC in the primer recognition complex might change on hydrolysis of @entity855 . We also suggest that the @entity994 - @entity1364 complex that remains attached to DNA might function to tether cyclin-CDK complexes to specific regions of the genome.

Cyclin-dependent kinase inhibitor XXXX modulates the DNA primer-template recognition complex.

@entity994 :: ('1026', 'Gene') :: ['p21'] @entity1364 :: ('5111', 'Gene') :: ['proliferating cell nuclear antigen', 'PCNA'] @entity1885 :: ('MESH:D001710', 'Chemical') :: ['biotin'] @entity12865 :: ('MESH:C022571', 'Chemical') :: ['ATPgammaS', "adenosine-5'-O-(3-thiotriphosphate)"] @entity17723 :: ('MESH:D000266', 'Chemical') :: ['adenyl-imidodiphosphate'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity994 :: (1026,Gene) :: ['p21']
