Understanding @entity5 clonality is critical to understanding the mechanisms involved in tumorigenesis and disease progression. In addition, understanding the clonal composition changes that occur within a @entity5 in response to certain micro-environment or treatments may lead to the design of more sophisticated and effective approaches to eradicate @entity5 cells. However, tracking @entity5 clonal sub-populations has been challenging due to the lack of distinguishable markers. To address this problem, a VDJ-seq protocol was created to trace the @entity2214 patterns of @entity1892 ( @entity1892 ) relapse by exploiting VDJ recombination and somatic hypermutation (SHM), two unique features of B cell lymphomas. In this protocol, Next-Generation sequencing (NGS) libraries with indexing potential were constructed from amplified rearranged @entity7639 ( @entity7639 ) VDJ region from pairs of primary diagnosis and relapse @entity1892 samples. On average more than half million VDJ sequences per sample were obtained after sequencing, which contain both VDJ rearrangement and SHM information. In addition, customized bioinformatics pipelines were developed to fully utilize sequence information for the characterization of @entity7639 -VDJ repertoire within these samples. Furthermore, the pipeline allows the reconstruction and comparison of the clonal architecture of individual @entity5 , which enables the examination of the clonal heterogeneity within the diagnosis @entity5 and deduction of @entity2214 patterns between diagnosis and @entity5 pairs. When applying this analysis to several diagnosis-relapse pairs, we uncovered key evidence that multiple distinctive @entity5 evolutionary patterns could lead to @entity1892 relapse. Additionally, this approach can be expanded into other clinical aspects, such as identification of minimal @entity66 , monitoring relapse progress and treatment response, and investigation of immune repertoires in non-lymphoma contexts.

VDJ-Seq: Deep Sequencing Analysis of Rearranged @entity7639 Gene to Reveal XXXX .

@entity2214 :: ('MESH:D007945', 'Disease') :: ['clonal evolution'] @entity66 :: ('MESH:D004194', 'Disease') :: ['residual disease'] @entity5 :: ('MESH:D009369', 'Disease') :: ['relapse tumor', 'tumor', 'tumors'] @entity1892 :: ('MESH:D016393', 'Disease') :: ['DLBCL', 'diffuse large B cell lymphoma'] @entity7639 :: ('3495', 'Gene') :: ['immunoglobulin heavy chain', 'IgH']
@entity1892 :: (MESH:D016393,Disease) :: ['Clonal Evolution Patterns of B Cell Lymphoma']
