UNASSIGNED: We studied the reproducibility and stability of limbal stem cell deficiency (LSCD) in @entity19 following controlled @entity267 . In one method, corneal and limbal epithelia were entirely removed with a 0.5  mm metal burr. In the other, limbus to limbus epithelial removal with the burr was followed by @entity130 to the limbus. These two methods were compared with a previously published one. Unwounded corneas were used as control. The corneas were examined monthly for three months by slit lamp with @entity941 staining. Immunofluorescence staining for @entity15251 and 8 on corneal wholemount and cross sections were performed to determine the phenotype of the epithelium. Mechanical shaving of the epithelium, with or without @entity130 , resulted in a reproducible state of LSCD marked by superficial neovascularization, reduce of @entity15251 expression and presence of goblet cells on the @entity267 . The phenotype was stable in 100% of the eyes up to at least three months. @entity130 produced a more severe phenotype with more significant stromal opacification. These @entity267 models may be useful for studying the mechanisms leading to limbal stem cell deficiency.

Stability of limbal stem cell deficiency after mechanical and @entity130 in XXXX .

@entity267 :: ('MESH:D065306', 'Disease') :: ['cornea', 'injuries to the corneal and limbal epithelia', 'corneal injury'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity941 :: ('MESH:D019793', 'Chemical') :: ['fluorescein'] @entity15251 :: ('268482(Tax:10090)', 'Gene') :: ['cytokeratin 12', 'keratin 12'] @entity130 :: ('MESH:D014947', 'Disease') :: ['Thermal injury', 'thermal injury']
@entity19 :: (10090,Species) :: ['mice']
