OBJECTIVE: To study the mechanism of docetaxel-induced apoptosis. METHODS: Morphological study, DNA gel electrophoresis, flow cytometry and fluorescin labeled @entity4404 to detect apoptosis, RT-PCR to detect the gene related with apoptosis. RESULTS: @entity1 @entity2 A549 cells treated with docetaxel induced cell cycle arrest at G2M phase, leading to apoptosis. The morphology of A549 showed nuclear chromatine condensation and fragmentation. Typical ladder pattern of DNA fragmentation was observed. Sub-G1 peak was found by flow cytometry. Transcription of Fas gene was enhanced, while no change in @entity517 and @entity588 genes. Annexin labeling results revealed the co-existence of cell apoptosis and @entity39 in docetaxel-treated A549 cells. CONCLUSION: Docetaxel induces apoptosis and @entity39 of @entity1 @entity2 . The induction of apoptosis may be related to expression of Fas.

[The mechanism of docetaxel-induced apoptosis in @entity1 XXXX cells].

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity588 :: ('596', 'Gene') :: ['bcl-2'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis'] @entity4404 :: ('308', 'Gene') :: ['Annexin V'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung cancer'] @entity517 :: ('4609', 'Gene') :: ['c-myc']
@entity2 :: (MESH:D008175,Disease) :: ['lung cancer']
