A new one-step ELISA was developed for the determination of the concentration of @entity1003 factor XIII subunit A (FXIII-A) in plasma and in cell lysates. Monoclonal antibodies directed against different epitopes on FXIII-A were used for the assay. The capture antibody was biotinylated on its @entity1735 moiety and the detection antibody was labelled with horseradish peroxidase. The antigen-antibody reaction was carried out in the well of a streptavidin-coated microplate. Complex formation with @entity35871 ( @entity35871 ) and association to fibrinogen did not influence the accessibility of the antibodies to FXIII-A. The method could be performed within 2 h and demonstrated good reproducibility, recovery and sensitivity. Plasma samples could be assayed after storage at -20 degrees C for at least 6 months. However, in the case of platelet lysates freezing and rethawing resulted in a significant loss of FXIII-A. FXIII-A concentrations measured in the plasma samples of healthy individuals and @entity1 correlated well with the concentrations of complexed plasma FXIII (A2B2) and with the results of FXIII activity measurements. A reference range of 46-82 fg/platelet was established for platelet FXIII-A.

Enzyme-linked immunosorbent assay for the determination of XXXX factor XIII A-subunit in plasma and in cell lysates.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity35871 :: ('2165', 'Gene') :: ['FXIII-B', 'FXIII subunit B'] @entity1003 :: ('MESH:D001778', 'Disease') :: ['blood coagulation'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['carbohydrate']
@entity1003 :: (MESH:D001778,Disease) :: ['blood coagulation']
