PURPOSE: To detect cell specific apoptosis factors, Fas and @entity12 , and the common intracellular apoptosis modulators, @entity9302 (ICE)-like protease ( @entity9302 ), @entity588 , @entity1836 and @entity589 in lens epithelial cells (LEC) of @entity1 @entity231 . To study the effects of Fas-stimulating monoclonal antibody on inhibition of LEC proliferation. METHODS: Reverse-transcriptase-polymerase chain reaction (RT-PCR) was used to detect Fas, @entity12 , @entity9302 , @entity588 , @entity1836 and @entity589 , after cDNA was synthesized from the total RNA isolated from @entity1 @entity231 LEC obtained by capsulotomy during @entity231 surgery. Fas-stimulating monoclonal antibody was added at the concentrations of 10, 30, 100, 300 and 1000 ng/ml to the incubation medium of @entity1 @entity231 LEC; and the specimens were incubated for 24 h at 37 degrees C with 5% @entity515 circulation and 100% humidity. The specimens were then stained with @entity5956 , and the number of apoptotic cells was counted. RESULTS: Fas, @entity9302 , @entity588 , @entity1836 and @entity589 mRNA were detected by RT-PCR. @entity12 mRNA was not detected by RT-PCR. At each concentration, Fas-stimulating monoclonal antibody significantly inhibited LEC proliferation. CONCLUSIONS: @entity1 @entity231 LEC expressed mRNA of Fas and various modulators of apoptosis pathways. Fas-stimulating monoclonal antibody may have the potential to prevent posterior capsule opacification after @entity231 surgery by inhibiting LEC proliferation.

Inhibition of lens epithelial cells by Fas-specific antibody activating Fas- XXXX system.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity589 :: ('581', 'Gene') :: ['Bax'] @entity588 :: ('596', 'Gene') :: ['Bcl-2'] @entity231 :: ('MESH:D002386', 'Disease') :: ['cataracts', 'cataractous', 'cataract'] @entity5956 :: ('MESH:C017807', 'Chemical') :: ['Hoechst 33342'] @entity1836 :: ('598', 'Gene') :: ['Bcl-xL'] @entity515 :: ('CHEBI:29334', 'Chemical') :: ['CO(2)'] @entity12 :: ('356', 'Gene') :: ['Fas ligand'] @entity9302 :: ('834', 'Gene') :: ['interleukin-1 beta converting enzyme', 'caspase 1']
@entity12 :: (356,Gene) :: ['Fas ligand']
