In @entity526 , the small regulatory noncoding RNA (sRNA) RyhB and the global ferric uptake regulator (Fur) mediate @entity68 acquisition and storage control. @entity68 is both essential and potentially toxic for most living organisms, making the precise maintenance of @entity68 homeostasis necessary for survival. While the roles of these regulators in @entity68 homeostasis have been well studied in a nonpathogenic @entity526 , their impact on the production of virulence-associated factors is still unknown for a pathogenic @entity526 . We thus investigated the roles of RyhB and Fur in @entity68 homeostasis and virulence of the uropathogenic @entity526 (UPEC) strain CFT073. In a @entity19 model of @entity293 ( @entity293 ), deletion of fur alone did not attenuate virulence, whereas a   ryhB mutant and a   fur   ryhB double mutant showed significantly reduced bladder colonization. The   fur mutant was more sensitive to oxidative stress and produced more of the siderophores enterobactin, salmochelins, and aerobactin than the wild-type strain. In contrast, while RyhB was not implicated in oxidative stress resistance, the   ryhB mutant produced lower levels of siderophores. This decrease was correlated with the downregulation of shiA (encoding a transporter of shikimate, a precursor of enterobactin and salmochelin biosynthesis) and iucD (involved in aerobactin biosynthesis) in this mutant grown in minimal medium or in @entity1 urine. iucD was also downregulated in bladders infected with the   ryhB mutant compared to those infected with the wild-type strain. Our results thus demonstrate that the sRNA RyhB is involved in production of @entity68 acquisition systems and colonization of the urinary tract by pathogenic @entity526 .

The small RNA RyhB contributes to siderophore production and virulence of uropathogenic XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity68 :: ('MESH:D007501', 'Chemical') :: ['iron', 'Iron'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity293 :: ('MESH:D014552', 'Disease') :: ['urinary tract infection', 'UTI'] @entity526 :: ('562', 'Species') :: ['Escherichia coli', 'E. coli', 'E. coli strain']
@entity526 :: (562,Species) :: ['Escherichia coli']
