The effect of @entity3800 on the oligosaccharide structure of follicle-stimulating hormone (FSH) isoforms was studied in adult male @entity35 treated with a potent @entity1176 (GnRH) antagonist. Animals were castrated (Cx), and 24 h later were treated with physiological doses of either @entity3033 (T) or @entity8048 ( @entity587 ) (0.25 mg/ @entity35 in @entity4336 oil, daily, s.c.) for 7 days. The antiandrogen @entity12194 (F; 5 mg/day/ @entity35 , twice a day, s.c.) was administered to either @entity1619 or Cx- @entity587 @entity35 to block @entity342 action. The GnRH antagonist @entity70538 ( @entity35951 mg/kg/day, s.c.) was injected to both Cx- and Cx- @entity587 treated rats. FSH serum levels reached normal values in Cx @entity35 treated with either T or @entity587 , whereas those treated with F retained Cx conditions. Both Cx-Ant and Cx-Ant- @entity587 treated animals presented normal serum FSH levels. Concanavalin A affinity chromatography was used to isolate pituitary FSH isoforms according to their @entity1735 inner structure. An increased proportion of FSH isoforms bearing complex-type oligosaccharides was observed in Cx @entity35 treated with T or @entity587 , whereas the proportion of these isoforms was reduced in Cx and @entity1619 -F or Cx- @entity587 -F @entity35 . These results demonstrate that functional @entity3800 are needed to complete the oligosaccharide processing of FSH. In addition, the proportion of the different pituitary FSH isoforms after @entity587 replacement was equivalent to that found when @entity1176 action was blocked, indicating that @entity3800 are involved in the regulation of @entity1735 incorporation to the FSH molecule by acting mainly at the pituitary level, independently of GnRH action.

Effect of dihydrotestosterone on pituitary follicle-stimulating hormone isoforms in adult male @entity35 treated with a XXXX antagonist.

@entity3033 :: ('MESH:D043343', 'Chemical') :: ['testosterone propionate'] @entity8048 :: ('MESH:C030445', 'Chemical') :: ['dihydrotestosterone propionate'] @entity1619 :: ('MESH:D015458', 'Disease') :: ['Cx-T'] @entity70538 :: ('MESH:C042691', 'Chemical') :: ['Org 30276'] @entity4336 :: ('381124', 'Species') :: ['corn'] @entity12194 :: ('MESH:D005485', 'Chemical') :: ['flutamide'] @entity35951 :: ('85333(Tax:10116)', 'Gene') :: ['Ant; 1'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['carbohydrate'] @entity342 :: ('CHEBI:50113', 'Chemical') :: ['androgen'] @entity1176 :: ('MESH:D007987', 'Chemical') :: ['GnRH', 'gonadotropin-releasing hormone'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity587 :: ('CHEBI:16330', 'Chemical') :: ['DHT'] @entity3800 :: ('MESH:D000728', 'Chemical') :: ['androgens']
@entity1176 :: (MESH:D007987,Chemical) :: ['gonadotropin-releasing hormone']
