UNASSIGNED: Neurons in the silkmoth @entity1315 ( @entity1315 ) are well characterized in terms of their morphology and odor-evoked firing activity. However, their intrinsic electrical properties including voltage-gated ionic currents and synaptic connectivity remain unclear. To address this, whole cell current- and voltage-clamp recordings were made from second-order projection neurons (PNs) and two morphological types of local interneurons (LNs) in the silkmoth @entity1315 . The two morphological types of LNs exhibited distinct physiological properties. One morphological type of LN showed a spiking response with a voltage-gated @entity1372 channel gene expression, whereas the other type of LN was nonspiking without a voltage-gated @entity1372 channel gene expression. Voltage-clamp experiments also revealed that both of two types of LNs as well as PNs possessed two types of voltage-gated @entity972 channels and @entity460 channels. In dual whole cell recordings of spiking LNs and PNs, activation of the @entity6841 elicited depolarization responses in the paired spiking LN, whereas activation of the spiking LN induced no substantial responses in the paired @entity6841 . However, simultaneous recording of a nonspiking LN and a @entity6841 showed that activation of the nonspiking LN induced hyperpolarization responses in the @entity6841 . We also observed bidirectional synaptic transmission via both chemical and electrical coupling in the pairs of spiking LNs. Thus our results indicate that there were two distinct types of LNs in the silkmoth @entity1315 , and their functional connectivity to PNs was substantially different. We propose distinct functional roles for these two different types of LNs in shaping odor-evoked firing activity in PNs.

Two types of local interneurons are distinguished by morphology, intrinsic membrane properties, and functional connectivity in the moth XXXX .

@entity6841 :: ('MESH:C565820', 'Disease') :: ['PN'] @entity1372 :: ('MESH:D012964', 'Chemical') :: ['sodium'] @entity1315 :: ('MESH:D004833', 'Disease') :: ['antennal lobe', 'AL'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity972 :: ('MESH:D011188', 'Chemical') :: ['potassium']
@entity1315 :: (MESH:D004833,Disease) :: ['antennal lobe']
