Telomerase is an RNA-dependent DNA polymerase that synthesizes TTAGGG telomeric DNA onto chromosome ends to compensate for sequence loss during DNA replication. It has been detected in 85-90% of all primary @entity1 @entity5 , implicating that the telomerase seems to be reactivated in @entity5 and that such activity may play a role in the tumorigenic process. The purpose of this study was to evaluate telomerase activity, @entity1 telomerase RNA ( @entity7066 ), and @entity2215 ( @entity2215 ) in @entity5 and to determine their potential relationships to clinicopathologic parameters. Frozen and corresponding @entity48909 -fixed @entity982 -embedded tissue samples were obtained from 51 @entity1 with @entity356 and analyzed for telomerase activity by using a TRAPeze ELISA kit. Tissue sections of all the samples were further investigated for @entity7066 and @entity2215 by in situ hybridization and a sensitive immunohistochemical technique, respectively. Telomerase activity was detected in 37 (73%) @entity5 . Telomerase positivity from methacarn-fixed @entity982 blocks was found to be 35% of that from frozen tissues. @entity7066 was overexpressed in 46 (90%) samples: 33/37 (89%) with and 13/14 (93%) without telomerase activation. Expression of @entity2215 was demonstrated in 40 (78%) cases: 30/37 (81%) with and 10/14 (71%) without telomerase. Telomerase activity correlated well with depth of invasion (P =.037) and @entity5 differentiation (P =.022), whereas @entity7066 significantly correlated with nodal @entity3 (P=.047) and @entity5 size (P=.023). These data suggest that reactivated telomerase may play a significant role in the tumorigenesis of @entity356 and may reflect, along with enhanced @entity7066 , the malignant potential of the @entity5 . It is noteworthy that methacarn-fixed tissue cannot as yet substitute for the frozen section in the TRAP assay.

Expression of telomerase activity, XXXX telomerase RNA, and @entity2215 in @entity356 .

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity48909 :: ('MESH:C046544', 'Chemical') :: ['methacarn'] @entity7066 :: ('7012', 'Gene') :: ['hTR'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'stomach cancer', 'cancers', 'tumors'] @entity982 :: ('MESH:D010232', 'Chemical') :: ['paraffin'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity2215 :: ('7015', 'Gene') :: ['TERT', 'telomerase reverse transcriptase'] @entity356 :: ('MESH:D013274', 'Disease') :: ['gastric cancer', 'gastric adenocarcinoma']
@entity1 :: (9606,Species) :: ['human']
