The influences of test variables on the outcome of susceptibility testing with @entity11794 were tested with isolates of Candida spp. and @entity54 . Among six growth conditions tested with a range of inoculum sizes, the highest control growth yields were obtained in Sabouraud broth for all fungi, followed by RPMI 1640 (pH 7) for Candida spp. and antibiotic medium 3 ( @entity40319 ) for A. fumigatus. RPMI 1640 gave unacceptably low growth yields with A. fumigatus. The @entity11794 MICs under these various conditions ranged over more than 4 twofold dilutions for 7 of 16 fungi tested when a 50% inhibition (50% inhibitory concentration [IC(50)]) endpoint was used and for 12 of 16 fungi tested when an 80% inhibition (IC(80)) endpoint was used. A multifactorial design to study the influences of six test variables on control growth and the MIC showed that, for 14 isolates of Candida spp., the @entity413 concentration and the medium composition were the most common factors significantly influencing both control growth yields and the MIC. For eight A. fumigatus isolates, incubation time (24 versus 48 h) and temperature (30 versus 35 degrees C) significantly affected control optical density (OD) values, while growth medium ( @entity40319 versus Sabouraud broth) was the most common process variable affecting the MICs. Tests with @entity40319 from three suppliers showed significant variations in control OD values related to supplier, but IC(50)s fell within a 2- or 3-dilution range for 19 (86%) of the 22 isolates tested. We recommend that, at present, @entity40319 is superior to RPMI 1640 for testing of the susceptibilities of both @entity375 and filamentous fungi to @entity11794 and that a minimum incubation time of 48 h is necessary to test A. fumigatus adequately.

Influences of methodological variables on susceptibility testing of @entity11794 against Candida species and XXXX .

@entity40319 :: ('MESH:C038773', 'Chemical') :: ['AM3'] @entity54 :: ('746128', 'Species') :: ['Aspergillus fumigatus'] @entity375 :: ('4932', 'Species') :: ['yeasts'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity11794 :: ('MESH:C105417', 'Chemical') :: ['caspofungin']
@entity54 :: (746128,Species) :: ['Aspergillus fumigatus']
