The most common subtype of @entity788 is @entity1124 ( @entity1124 ). @entity1124 resembles ductal cells morphologically. To study pancreatic ductal cell (PDC) and @entity788 ( @entity788 )/ @entity1124 biology, it is essential to have reliable in vitro culture conditions. Here we describe a methodology to isolate, culture, and passage PDCs and duct-like cells from the @entity19 pancreas. It can be used to isolate cells from genetically engineered @entity19 models (GEMMs), providing a valuable tool to study disease models in vitro to complement in vivo findings. The culture conditions allow epithelial cells to outgrow fibroblast and other "contaminating" cell types within a few passages. However, the resulting cultures, although mostly epithelial, are not completely devoid of fibroblasts. Regardless, this protocol provides guidelines for a robust in vitro culture system to isolate, maintain, and expand primary pancreatic ductal epithelial cells. It can be applied to virtually all GEMMs of @entity660 and @entity5 that arise from ductal structures. Because most @entity189 resemble ductal structures, this protocol has utility in the study of other @entity5 in addition to @entity1124 , such as @entity0 .

Culturing primary XXXX pancreatic ductal cells.

@entity788 :: ('MESH:D010190', 'Disease') :: ['pancreatic intraepithelial neoplasia', 'PanIN', 'pancreatic cancer'] @entity1124 :: ('MESH:D021441', 'Disease') :: ['PDAC', 'pancreatic ductal adenocarcinoma'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast and prostate cancers'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancers'] @entity660 :: ('MESH:D010182', 'Disease') :: ['pancreatic disease and other diseases'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinomas']
@entity19 :: (10090,Species) :: ['mouse']
