UNASSIGNED: Aristolochic acids (AA) are nephrotoxic agents found in Aristolochia species whose consumption leads to the onset of a progressive tubulointerstitial @entity82 . This AA- @entity8 was first reported during the Belgian outbreak of the 1990's in which more than a hundred @entity1 consumed slimming pills containing an Aristolochia species and Magnolia officinalis. The @entity1 developed an @entity454 requiring dialysis or transplantation. @entity1060 and @entity1059 are bioactive compounds from M. officinalis known for their potent antioxidant activity. As they can alleviate oxidative stress, we investigated their respective effects on AA-mediated tubulotoxicity using @entity1  cells. @entity1060 and @entity1059 were able to reduce the oxidative stress associated with AA-treatment. @entity137 alleviation was further investigated and overall cell viability measurements unexpectedly revealed that both compounds worsened the survival of AA-treated cells. Flow cytometry analyses of @entity4404 /PI stained cells indicated that the @entity16296 efficiently prevented AA-induced apoptosis; but favored @entity39 . Microscopy observations highlighted extensive vacuolization; other types of cell death, including autophagy, paraptosis or accelerated senescence were excluded. Ki-67 index and cell cycle analysis indicated that both @entity1060 and @entity1059 inhibited proliferation by blocking the cell cycle at the G1 phase; they also prevented the AA-induced G2/M arrest.

New in  vitro insights on a cell death pathway induced by @entity1060 and XXXX in aristolochic acid tubulotoxicity.

@entity1 :: ('9606', 'Species') :: ['patients', 'HK-2'] @entity8 :: ('MESH:D007674', 'Disease') :: ['nephropathy'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis'] @entity137 :: ('MESH:D064420', 'Disease') :: ['Cytotoxicity'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis'] @entity4404 :: ('308', 'Gene') :: ['annexin V'] @entity16296 :: ('MESH:D017705', 'Chemical') :: ['lignans'] @entity1060 :: ('MESH:C005498', 'Chemical') :: ['magnolol', 'Magnolol'] @entity1059 :: ('MESH:C005499', 'Chemical') :: ['honokiol'] @entity454 :: ('MESH:D007676', 'Disease') :: ['end-stage kidney disease']
@entity1059 :: (MESH:C005499,Chemical) :: ['honokiol']
