The distribution of several @entity1447 (P-450) isoenzymes between proximal tubular (PT) and distal tubular (DT) cells of the @entity35 kidney was determined. Western blot analysis of microsomes prepared from liver and kidney cortical homogenates revealed that @entity11920 protein was expressed in @entity35 kidney microsomes at approximately 10% of hepatic levels. Microsomes from renal cortical, PT, and DT cells all expressed @entity11920 , with DT microsomes expressing slightly higher levels than PT microsomes. In contrast, @entity16506 hydroxylation activity was markedly higher in microsomes from PT cells than in those from DT cells. Northern blot analysis of total RNA from PT and DT cells exhibited a pattern of @entity11920 mRNA distribution similar to that of @entity11920 protein. @entity11042 protein expression in renal cortical microsomes was approximately 10% of that in liver microsomes but was significantly higher in microsomes from PT cells than in those from DT cells. @entity69062 was not detected in microsomes from either cortical, PT, or DT cells, but was detected in microsomes isolated from total liver or kidney cortical homogenates. @entity4921 /2 expression was detected in all tissues tested. The peroxisomal proliferator clofibrate enhanced the level of @entity4921 /2 in microsomes from both total liver and kidney cortical homogenates but not in microsomes from cortical, PT, or DT cells. @entity69063 protein and CYP4A mRNA expression were detected in microsomes from total liver and kidney cortical homogenates and from renal cortical, PT, and DT cells using Western and Northern blot analyses, respectively. @entity27146 hydroxylation activity, an indicator of CYP4A, was comparable in PT and DT cells. Clofibrate elevation of CYP4A in cortical, PT, and DT microsomes was not as great as that detected in total kidney cortical microsomes. These results establish the distribution of several P-450 isoenzymes between different cell populations of the @entity35 kidney. Furthermore, these results present evidence that the level of induction of certain P-450 isoenzymes in the kidney is cell type-specific.

Cellular distribution of cytochromes P-450 in the XXXX kidney.

@entity1447 :: ('25251(Tax:10116)', 'Gene') :: ['cytochrome P-450'] @entity69063 :: ('24306;170509', 'Gene') :: ['CYP4A2/3'] @entity69062 :: ('25642;266682', 'Gene') :: ['CYP3A1/2'] @entity16506 :: ('MESH:D002753', 'Chemical') :: ['chlorzoxazone'] @entity4921 :: ('24300(Tax:10116)', 'Gene') :: ['CYP2B1'] @entity11042 :: ('29277(Tax:10116)', 'Gene') :: ['CYP2C11'] @entity27146 :: ('MESH:C030358', 'Chemical') :: ['Lauric acid'] @entity11920 :: ('25086(Tax:10116)', 'Gene') :: ['CYP2E1'] @entity35 :: ('10116', 'Species') :: ['rat']
@entity35 :: (10116,Species) :: ['rat']
