UNASSIGNED: A novel rhabdovirus was isolated from the serum of a healthy Japanese wild boar ( @entity1707 leucomystax) and identified using the rapid determination system for viral nucleic acid sequences ( @entity21822 ), next-generation sequencing, and electron microscopy. The virus was tentatively named @entity1707 rhabdovirus 1 (WBRV1). Phylogenetic analysis of the entire genome sequence indicated that WBRV1 is closely related to @entity69300 ( @entity15510 ), which was isolated from cultured cells of hepatocellular @entity189 tissue of tree shrew. @entity15510 has not been assigned to any genus of Rhabdoviridae till date. Analysis of the L gene indicated that WBRV1 belongs to the genus Vesiculovirus. These observations suggest that both @entity15510 and WBRV1 belong to a new genus of Rhabdoviridae. Next-generation genome sequencing of WBRV1 revealed 5 open reading frames of 1329, 765, 627, 1629, and 6336 bases in length. The WBRV1 gene sequences are similar to those of other rhabdoviruses. Epizootiological analysis of a population of @entity1707 in Wakayama prefecture in Japan indicated that 6.5% were positive for the WBRV1 gene and 52% were positive for WBRV1-neutralizing antibodies. Furthermore, such viral neutralizing antibodies were found in @entity12526 in another prefecture. WBRV1 was inoculated intranasally and intraperitoneally into @entity915 and BALB/c @entity19 and viral RNA was detected in @entity915 @entity19 , suggesting that WBRV1 can replicate in immunocompromised @entity19 . These results indicate this novel virus is endemic in wild animals and livestock in Japan.

Isolation and characterization of a novel Rhabdovirus from a XXXX ( @entity1707 ) in Japan.

@entity1707 :: ('9823', 'Species') :: ['wild boar', 'wild boars', 'Sus scrofa'] @entity15510 :: ('12295', 'Species') :: ['TRV'] @entity12526 :: ('9825', 'Species') :: ['domestic pigs'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinoma'] @entity915 :: ('MESH:D053632', 'Disease') :: ['SCID'] @entity69300 :: ('318558', 'Species') :: ['Tupaia rhabdovirus'] @entity21822 :: ('10991', 'Species') :: ['RDV']
@entity1707 :: (9823,Species) :: ['wild boar']
