UNASSIGNED: @entity15743 is known to be abnormally expressed in @entity157  ( @entity157 ) cells, and the overexpression of @entity15743 inhibits the proliferation, invasion and @entity3 of various @entity5 cells. MicroRNAs (miRNAs or miRs) are closely related to the proliferation, invasion and @entity3 of @entity157 . The present study aimed to explore the effects of @entity15743 on the proliferation, invasion and @entity3 of @entity157 cells, and to elucidate the mechanisms involved. A cell line with moderate levels of @entity15743 expression was selected from the SMMC-7721, HepG2, SK-Hep-1 and @entity5959 @entity157 cells and validated by reverse transcription-PCR  (RT-PCR). The effects of @entity15743 on @entity157 cell viability were determined by @entity98 assay; cell migration and invasion were determined by Transwell cell invasion assay, and apoptosis was determined by flow cytometery  (FCM). Western blot analysis was used to measure the expression levels of the apoptosis-related proteins, @entity589 , @entity588 , @entity1476 , @entity1495 , as well as to examine epithelial-mesenchymal transition  (EMT), and @entity5962 , @entity2277 , and @entity4357  3-kinase  ( @entity10412 )/ @entity584 signaling pathway-related protein expression. The mRNA expression levels of @entity15743 were highest in the SMMC-7721 cells and lowest in the SK-Hep  -1 and @entity5959 cells; moderate levels were observed in the HepG2 cells. The HepG2 cell line was selected as the cell line for use in the follow-up experiments, where we measured cell viability, and the expression of @entity10412 / @entity584 , @entity589 , @entity588 , @entity1476 and @entity1495 , and @entity5962 and @entity2277 . @entity15743 overexpression significantly inhibited the proliferation, invasion and @entity3 of the HepG2 cells, as shown by western blot anlaysis. This was achieved through the upregulation of @entity589 expression, the downregulation of @entity588 expression and the decrease in the expression of @entity1476 and @entity1495 . In addition the epxression of @entity5962 increased and @entity2277 expression decreased. @entity15743 overexpression inhibited @entity10412 expression and @entity584 phosphorylation. The results of this study suggest that the overexpression of @entity15743 inhibits the proliferation, invasion and @entity3 of HepG2 @entity157 cells, and that these effects are associated with the @entity10412 / @entity584 signaling pathway.

Suppressive effects of microRNA-16 on the proliferation, invasion and XXXX of @entity157 cells.

@entity589 :: ('581', 'Gene') :: ['Bax'] @entity588 :: ('596', 'Gene') :: ['Bcl-2'] @entity1476 :: ('4313', 'Gene') :: ['MMP-2', 'matrix metalloproteinase  (MMP)-2'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity5962 :: ('999', 'Gene') :: ['E-cadherin'] @entity584 :: ('207', 'Gene') :: ['Akt'] @entity5959 :: ('284424', 'Gene') :: ['Huh  -7'] @entity15743 :: ('51573', 'Gene') :: ['miR-16', 'miR  -16'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity10412 :: ('5293', 'Gene') :: ['PI3K', 'PI3k'] @entity2277 :: ('7431', 'Gene') :: ['vimentin'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity1495 :: ('4318', 'Gene') :: ['MMP-9'] @entity157 :: ('MESH:D006528', 'Disease') :: ['HCC', 'hepatocellular carcinoma'] @entity4357 :: ('MESH:D010716', 'Chemical') :: ['phosphatidylinositol']
@entity3 :: (MESH:D009362,Disease) :: ['metastasis']
