Toll-like receptor (TLR)-mediated signalling plays a role in @entity1779 /reperfusion (I/R) injury. Modulation of TLRs has been reported to protect against cerebral I/R injury. This study examined whether modulation of @entity15905 with poly (I:C) will induce protection against cerebral I/R injury. @entity19 were treated with or without Poly (I:C) (n  =  8/group) 1  hr prior to @entity1779 (60  min.) followed by reperfusion (24  hrs). Poly (I:C) pre-treatment significantly reduced the @entity1188 volume by 57.2% compared with untreated I/R @entity19 . Therapeutic administration of Poly (I:C), administered 30  min. after @entity1779 , markedly decreased @entity1188 volume by 34.9%. However, Poly (I:C)-induced protection was lost in @entity15905 knockout @entity19 . In poly (I:C)-treated @entity19 , there was less @entity528 in the hippocampus compared with untreated I/R @entity19 . Poly (I:C) treatment induced @entity14055 phosphorylation, but it inhibited NF-kB activation in the brain. Poly (I:C) also decreased I/R-induced apoptosis by attenuation of Fas/ @entity9699 -mediated apoptotic signalling. In addition, Poly (I:C) treatment decreased microglial cell @entity185 activity. In vitro data showed that Poly (I:C) prevented @entity519 /reoxygenation (H/R)-induced interaction between Fas and @entity51424 as well as @entity34645 activation in microglial cells. Importantly, Poly (I:C) treatment induced co-association between @entity15905 and Fas. Our data suggest that Poly (I:C) decreases in cerebral I/R injury via @entity15905 which associates with Fas, thereby preventing the interaction of Fas and @entity51424 , as well as microglial cell @entity34645 activities. We conclude that @entity15905 modulation by Poly (I:C) could be a potential approach for protection against @entity64 .

Poly (I:C) therapy decreases XXXX /reperfusion injury via @entity15905 -mediated prevention of Fas/ @entity51424 interaction.

@entity34645 :: ('12367;12370', 'Gene') :: ['caspase-3 and -8'] @entity64 :: ('MESH:D020521', 'Disease') :: ['ischaemic stroke'] @entity19 :: ('10090', 'Species') :: ['mice', 'Mice'] @entity185 :: ('12367(Tax:10090)', 'Gene') :: ['caspase-3'] @entity14055 :: ('54131(Tax:10090)', 'Gene') :: ['IRF3'] @entity519 :: ('MESH:D000860', 'Disease') :: ['hypoxia'] @entity1188 :: ('MESH:D007238', 'Disease') :: ['infarct'] @entity9699 :: ('14103(Tax:10090)', 'Gene') :: ['FasL'] @entity1779 :: ('MESH:D002543', 'Disease') :: ['cerebral ischaemia'] @entity15905 :: ('142980(Tax:10090)', 'Gene') :: ['TLR3'] @entity528 :: ('MESH:D009410', 'Disease') :: ['neuronal damage'] @entity51424 :: ('14082(Tax:10090)', 'Gene') :: ['FADD']
@entity1779 :: (MESH:D002543,Disease) :: ['cerebral ischaemia']
