INTRODUCTION: Previously, we established a simple method for deriving mesenchymal stem cells (MSCs) from @entity1 induced pluripotent stem cells (iPSC-MSCs). These iPSC-MSCs were capable of forming osteogenic structures in scaffolds and nanofibers. The objective of this study is to systematically characterize the mesenchymal characteristics of the iPSC-MSCs by comparing them to bone marrow-derived MSCs (BM-MSCs). METHODS: Two iPSC-MSC lines (named as mRNA-iPSC-MSC-YL001 and lenti-iPSC-MSC-A001) and one BM-MSC line were used for the study. Cell proliferation, presence of mesenchymal surface markers, tri-lineage differentiation capability (osteogenesis, chondrogenesis, adipogenesis), and expression of " @entity3308 " genes were analyzed in these MSC lines. RESULTS: The iPSC-MSCs were similar to BM-MSCs in terms of cell morphology (fibroblast-like) and surface antigen profile: @entity2393 +, @entity1759 +, @entity12359 +, @entity5399 +, CD105+, @entity9536 -, @entity5750 -, @entity2279 -, @entity2280 -, @entity1195 - and HLA-DR-. A faster proliferative capability was seen in both iPSC-MSCs lines compared to the BM-MSCs. The iPSC-MSCs showed adequate capacity of osteogenesis and chondrogenesis compared to the BM-MSCs, while less adipogenic potential was found in the iPSC-MSCs. The iPSC-MSCs and the tri-lineage differentiated cells (osteoblasts, chondrocytes, adipocytes) all lack expression of " @entity3308 " genes: @entity4729 , @entity4731 , @entity21608 , @entity9637 , @entity50317 , @entity62723 , @entity15894 , @entity26534 , and SAL4. CONCLUSIONS: The MSCs derived from @entity1 iPSCs with our method have advanced proliferation capability and adequate osteogenic and chondrogenic properties compared to BM-MSCs. However, the iPSC-MSCs were less efficient in their adipogenicity, suggesting that further modifications should be applied to our method to derive iPSC-MSCs more closely resembling the na  ve BM-MSCs if necessary.

Mesenchymal stem cells derived from XXXX induced pluripotent stem cells retain adequate osteogenicity and chondrogenicity but less adipogenicity.

@entity1 :: ('9606', 'Species') :: ['human'] @entity26534 :: ('79727', 'Gene') :: ['LIN28a'] @entity3308 :: ('MESH:D020295', 'Disease') :: ['stemness'] @entity4729 :: ('5460', 'Gene') :: ['OCT4'] @entity5750 :: ('929', 'Gene') :: ['CD14'] @entity9637 :: ('6998', 'Gene') :: ['CRIPTO'] @entity1195 :: ('5788', 'Gene') :: ['CD45'] @entity1759 :: ('960', 'Gene') :: ['CD44'] @entity2279 :: ('5175', 'Gene') :: ['CD31'] @entity5399 :: ('7070', 'Gene') :: ['CD90'] @entity4731 :: ('6657', 'Gene') :: ['SOX2'] @entity21608 :: ('9573', 'Gene') :: ['GDF3'] @entity12359 :: ('4907', 'Gene') :: ['CD73'] @entity2393 :: ('3688', 'Gene') :: ['CD29'] @entity9536 :: ('3684', 'Gene') :: ['CD11b'] @entity2280 :: ('947', 'Gene') :: ['CD34'] @entity62723 :: ('55211', 'Gene') :: ['DPPA4'] @entity50317 :: ('8433', 'Gene') :: ['UTF1'] @entity15894 :: ('1789', 'Gene') :: ['DNMT3B']
@entity1 :: (9606,Species) :: ['human']
