L-type @entity460 channels can modulate neuronal transduction in the spinal cord. However, their role in noxious information processing in animals that are physiologically intact has not been elucidated. We evaluated the effects of L-type @entity460 channel blockers @entity3988 and @entity1855 on somatic and visceral nociception at the level of the spinal cord. Intrathecal catheters were inserted at the L4-5 level in @entity35 . The tail flick (TF) test and @entity14 ( @entity14 ) test were used to assess somatic and visceral antinociceptive effects, respectively. Motor function was assessed by posture and muscle tone in the limbs. TF latency and @entity14 threshold were measured before and for 180 min after the intrathecal administration of @entity1855 (50, 100, 300, and 500 microg), @entity3988 (100, 300, 500, and 1000 microg), or isotonic @entity2827 solution. The percent maximal possible effect (%MPE) was calculated by transforming response threshold in TF and @entity14 tests. Intrathecally administered @entity3988 or @entity1855 increased both TF latency and @entity14 threshold in a dose-dependent fashion. Isotonic @entity2827 solution, @entity3988 100 microg, and @entity1855 50 microg did not increase %MPE in either test. @entity3988 300 or 500 microg or @entity1855 300 or 500 microg significantly (P < 0.05) increased %MPE, with the peak effects 5 min after administration and short-duration antinociception. %MPE was 100% until 15 min after the administration of @entity3988 1000 microg, and significant antinociception continued until 180 min in the TF test. Motor paralysis was observed after the administration of the larger dose of each drug. We demonstrated that intrathecally administered L-type @entity460 channel blockers @entity3988 or @entity1855 produced both somatic and visceral antinociception and motor block dose-dependently. IMPLICATIONS: We examined the effects of intrathecally administered L-type @entity460 channel blockers @entity3988 and @entity1855 on somatic and visceral nociception in @entity35 . L-type @entity460 channel blockers produced antinociceptive effects, suggesting a possible clinical application to control @entity158 .

Antinociceptive effects of intrathecal L-type XXXX channel blockers on visceral and somatic stimuli in the @entity35 .

@entity1855 :: ('MESH:D014700', 'Chemical') :: ['verapamil'] @entity3988 :: ('MESH:D004110', 'Chemical') :: ['diltiazem', 'Diltiazem'] @entity14 :: ('MESH:D015179', 'Disease') :: ['CD', 'colorectal distension'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity35 :: ('10116', 'Species') :: ['Sprague-Dawley rats', 'rats'] @entity158 :: ('MESH:D010146', 'Disease') :: ['pain'] @entity2827 :: ('MESH:D012965', 'Chemical') :: ['sodium chloride']
@entity460 :: (MESH:D002118,Chemical) :: ['calcium']
