UNASSIGNED: @entity310 -, @entity68885 -targeting drugs are highly effective in the treatment of @entity1569 . However, the precise molecular mechanism remains unknown. In psoriatic skin the presence of Langerhans cells (LC) is reduced, but the role of LC is poorly understood. The purpose of this study was to investigate the impact of @entity310 and @entity4452 / @entity4155 on the presence of LC in the skin during treatment. Therefore, psoriatic skin was investigated before and after 4 days of adalimumab or ustekinumab treatment. Furthermore, @entity310 and @entity4155 stimulation was investigated in an ex vivo model of epidermis and dermis from healthy normal skin kept in cultures at an air-liquid interphase for 4 days. In a gene array analysis we found that the two LC markers, @entity4896 and @entity9184 , were among the most up- or downregulated genes in psoriatic skin after anti- @entity310 therapy. Validation showed that both mRNA expression and protein level followed the same pattern and became significantly upregulated after 4 days of treatment. No changes were seen after ustekinumab treatment. In the ex vivo skin model, a decrease in the @entity4896 level was seen after @entity310 stimulation and it was caused by LC migration from epidermis. No response in LC migration was seen after @entity4155 stimulation. Taken together, we demonstrated that changes in the LC level in epidermis precede the histological and clinical changes during adalimumab treatment in psoriatic skin. Furthermore, @entity310 plays a prominent role in orchestrating LC migration in the skin. This seems not to be the true for the @entity4452 / @entity4155 pathway. This article is protected by copyright. All rights reserved.

Langerhans cell markers @entity4896 and XXXX are the most rapidly responding genes in lesional psoriatic skin following adalimumab treatment.

@entity9184 :: ('50489', 'Gene') :: ['CD207'] @entity1569 :: ('MESH:D011565', 'Disease') :: ['psoriasis'] @entity310 :: ('7124', 'Gene') :: ['TNFa'] @entity68885 :: ('51561;3605', 'Gene') :: ['IL-23- and IL-17'] @entity4155 :: ('3605', 'Gene') :: ['IL-17', 'IL-17A'] @entity4896 :: ('909', 'Gene') :: ['CD1a'] @entity4452 :: ('51561', 'Gene') :: ['IL-23']
@entity9184 :: (50489,Gene) :: ['CD207']
