Using endogenous lipid substrates, assays of lipid phosphorylation indicated that neuronal nuclei had a considerable superiority in @entity8558 (PA) formation when compared with homogenates and other subfractions of cerebral cortex. This predominance of neuronal nuclear PA labelling was linked to a sizable pool of nuclear @entity13286 that expanded significantly with incubation. PA was also the dominant product of neuronal nuclear lipid phosphorylation reactions. Nuclear envelope preparations and the parent neuronal nuclei showed specific rates of PA formation that were comparable, based upon membrane phospholipid contents. As well, using an exogenous @entity2366 substrate, the distribution of @entity2366 kinase activities closely followed phospholipid contents of subfractions derived from the neuronal nucleus during envelope preparation. This evidence suggested an association between @entity2366 kinase and the neuronal nuclear envelope. Nuclear PA formation increased in the presence of @entity5597 , while @entity5597 decreased PA formation in other subfractions. Likely @entity5597 exerted its effect on nuclear @entity2366 kinase, as @entity5597 did not elevate levels of nuclear @entity13286 . @entity7985 was present in the nuclei and inhibitors of this enzyme did decrease PA formation, indicating @entity13286 from inositides as substrates for nuclear @entity2366 kinase. The nuclear envelope fraction had a considerably lower specific @entity7985 activity than the parent nuclei, and showed an activation of PA formation by @entity5597 , but a less efficient handling of the exogenous @entity2366 substrate. It is possible that @entity7985 and @entity2366 kinase are closely situated within the neuronal nuclei, and a loss of the former activity may compromise the latter.

XXXX is the prominent product of endogenous neuronal nuclear lipid phosphorylation, an activity enhanced by @entity5597 , linked to phospholipase C and associated with the nuclear envelope.

@entity2366 :: ('CHEBI:41847', 'Chemical') :: ['diacylglycerol'] @entity8558 :: ('MESH:D010712', 'Chemical') :: ['phosphatidic acid'] @entity7985 :: ('5335', 'Gene') :: ['phosphoinositidase C', 'Phosphoinositidase C'] @entity5597 :: ('MESH:D013110', 'Chemical') :: ['sphingosine'] @entity13286 :: ('MESH:D004075', 'Chemical') :: ['diacylglycerols']
@entity8558 :: (MESH:D010712,Chemical) :: ['Phosphatidic acid']
