PURPOSE: To determine the effects of @entity1457 treatment on cold @entity231 formation in @entity291 lenses. METHODS: A laser scanning system (ScanTox) was used to analyze the optical quality of @entity291 lenses during a cooling and warming cycle. @entity1880 , a compound that prevents new protein synthesis was used to inhibit inducible @entity1457 protein 70 (Hsp70) production during heat stress. @entity1880 was also used to verify that the induction of Hsp70 takes place in lenses during heat stress. Western blots determined the relative accumulation of Hsp70 in @entity1133 soluble lens fractions. Lenses from animals approximately 2 years of age (n=60) were used in this experiment. RESULTS: The decrease in relative light transmittance during cold @entity231 varies for each group of lenses, with the greatest decrease appearing in the @entity1457 group in culture medium and the least in the control groups and the @entity1457 group with @entity1880 . The primary result is that @entity1457 were most affected by the cold @entity231 (57% decrease in intensity of refracted beam), and that this effect is prevented by @entity1880 . Western blot results show an increase of Hsp70 with @entity1457 in the @entity1133 soluble lens fractions. CONCLUSIONS: The results show that @entity1457 treatment increased both light scattering and the presence of Hsp70. Also cycloheximde prevented both the @entity1457 effect and the expression of Hsp70 in @entity291 lenses.

Hsp70 in XXXX lenses during temperature stress.

@entity231 :: ('MESH:D002386', 'Disease') :: ['cataract'] @entity1133 :: ('MESH:D014508', 'Chemical') :: ['urea'] @entity1880 :: ('MESH:D003513', 'Chemical') :: ['cycloheximide', 'Cycloheximide'] @entity1457 :: ('MESH:D012769', 'Disease') :: ['heat shock lenses', 'heat shock'] @entity291 :: ('9913', 'Species') :: ['bovine']
@entity291 :: (9913,Species) :: ['bovine']
