PURPOSE: Oxidative stress is known to disrupt the integrity of @entity989 ( @entity989 ) tight junctions. The goal of this study is to evaluate the effect of @entity2995 (TA) on the junctional integrity of @entity989 under oxidative stress and to identify the underlying mechanisms. METHODS: Second passage porcine @entity989 cells were cultured on 6-well membrane inserts until 4 weeks after reaching confluence. Cells were incubated with TA (10(-5) M) for 30 min. @entity2823 -containing medium was added to the upper chamber (cell's apical side). The cells were then challenged with 1 mM @entity273 ( @entity274 ). After 5 h, the fluorescence intensity of the medium from lower chamber (cell's basolateral side) was measured using a fluorescence spectrofluorophotometer. This transepithelial flux of @entity2823 -dextran was measured until the 21st day. The immunolocalization of @entity11177 and F-actin was examined with fluorescence microscope. Reduced @entity2041 ( @entity2197 )/ @entity9492 ( @entity9492 ) ratio was determined by a colorimetric assay kit. RESULTS: Non-lethal oxidative stress by @entity274 increased transepithelial flux of @entity2823 -dextran significantly. TA inhibited this increase and preserved the lower flux through the whole experimental period. This permeability change by @entity274 was reversible and recovered to the normal level within 3 weeks. In immunohistological study, @entity274 reduced linear @entity11177 staining at the cell border and increased actin stress fibers. TA prevented @entity274 -induced disruption of junctional assembly of @entity11177 and F-actin. @entity2041 assay demonstrated that intracellular @entity2197 / @entity9492 ratio decreased significantly with @entity274 , while TA preserved this ratio by up-regulating @entity2197 synthesis. CONCLUSIONS: TA has a protective effect against oxidative stress-induced disruption of @entity989 tight junction by preserving cellular redox state.

XXXX prevents oxidative stress-induced tight junction disruption of retinal pigment epithelial cells.

@entity2995 :: ('MESH:D014222', 'Chemical') :: ['triamcinolone acetonide'] @entity9492 :: ('MESH:D019803', 'Chemical') :: ['GSSG', 'oxidized glutathione'] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H(2)O(2)'] @entity11177 :: ('100506658', 'Gene') :: ['occludin'] @entity2041 :: ('MESH:D005978', 'Chemical') :: ['glutathione', 'Glutathione'] @entity2197 :: ('CHEBI:16856', 'Chemical') :: ['GSH'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['Hydrogen Peroxide'] @entity2823 :: ('MESH:D016650', 'Chemical') :: ['FITC'] @entity989 :: ('MESH:D012164', 'Disease') :: ['retinal pigment epithelium', 'RPE']
@entity2995 :: (MESH:D014222,Chemical) :: ['Triamcinolone acetonide']
