We previously proposed a new assay using the cytokinesis-block micronucleus (MN) technique to estimate the fraction of cells undergoing mitosis in vitro [dividing fraction (DF)], potential doubling time (Tpot), and radiosensitivity (in terms of MN frequency) of @entity1 @entity5 . In the present study, we applied this technique to primary @entity2 to evaluate their biological characteristics, and the assay results for the proliferative activity were compared with the treatment outcome. @entity5 tissues were disaggregated to single cells, which were cultured in the presence of cytochalasin B after (or without) radiation. At intervals, the proportion of multinucleate cells (its maximum value is the DF), the average number of nuclei/cell, and MNs in binucleate cells were scored. The Tpot was the extrapolated time for the nuclei:cell ratio to reach 2.0. Of the 71 @entity5 samples obtained, the DF and Tpot were evaluable in 61 (86%), and the MN frequency was evaluable in 52 (73%). The median DF and Tpot values were 23% and 7.7 days, respectively, for @entity420 (n = 41), 26% and 8.9 days for @entity957 (n = 13), 27% and 6.5 days for @entity152 (n = 3), and 30% and 7.0 days for @entity4153 (n = 4). There was no significant difference in the mean DF or Tpot values according to the histological type or disease stage. The mean MN frequency after 2 Gy of radiation (minus the 0 Gy frequency) was 0.15 for @entity420 , 0.17 for @entity957 , 0.16 for @entity152 , and 0.20 for @entity4153 . The MN frequency after radiation was positively correlated with both the DF and the baseline (at 0 Gy) MN frequency. In @entity1149 , a DF above the median was associated with an increased recurrence rate after operation, and the Tpot was correlated with the time until relapse in @entity1 who developed recurrence. Although the clinical significance of the MN frequency needs to be clarified in future studies, the DF and Tpot determined by this assay appear to be good parameters of @entity5 proliferative activity.

Proliferative activity and micronucleus frequency after radiation of XXXX cells as assessed by the cytokinesis-block method and their relationship to clinical outcome.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity4153 :: ('MESH:D018288', 'Disease') :: ['small cell carcinoma'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'Tumor', 'tumors'] @entity957 :: ('MESH:D002294', 'Disease') :: ['squamous cell carcinoma'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung cancers'] @entity1149 :: ('MESH:D002289', 'Disease') :: ['non-small cell lung cancer'] @entity152 :: ('MESH:D018287', 'Disease') :: ['large cell carcinoma'] @entity420 :: ('MESH:D000230', 'Disease') :: ['adenocarcinoma']
@entity2 :: (MESH:D008175,Disease) :: ['lung cancer']
