UNASSIGNED: Osteoprotegrin (OPG), receptor activator of nuclear factor kB (RANK) and @entity5298 ( @entity5298 ) are signal transducers which have pleiotropic actions. Each @entity5 necrosis factor receptor superfamily member has unique structural attributes which directly couples them to signalling pathways involved in cell proliferation, differentiation and survival. Previous studies have clinically linked OPG, RANK and @entity5298 to increasing @entity5 burden, metastatic bone involvement and @entity172 status. This study aimed to establish the potential implications of targeting endogenously produced OPG and RANK in the @entity0 cell line MDA-MB  -231 in vitro. Subsequently this study also aimed to explore the potential links between these molecules with regards to @entity11968 ( @entity11968 ) signalling and extracted bone proteins (BME). OPG and RANK expression was successfully suppressed using hammerhead ribozyme technology. Subsequently effects were explored in MDA-MB  -231 cell proliferation, matrix adhesion, migration and invasion in  vitro function assays. Reduced OPG expression resulted in @entity0 cell migration and invasion. These increases, particularly invasion, appeared to however be reduced under the influence of the exogenous stimuli ( @entity11968 and BME). In contrast, suppression of RANK in MDA-MB  -231 @entity0 cells resulted in decreased @entity5 cell proliferation, matrix-adhesion, motility and invasion with little cumulative effect being noted after the addition of exogenous stimuli. The complexity of the bone environment underpins the vast number of soluble factors and signalling pathways which can influence @entity5 behaviour and progression. Further work into elucidating all the pathways affected could potentially lead to better identification of those @entity1 most at risk.

Importance of osteoprotegrin and receptor activator of nuclear factor kB in @entity0 response to XXXX and the bone microenvironment in vitro.

@entity172 :: ('MESH:D004967', 'Chemical') :: ['estrogen'] @entity1 :: ('9606', 'Species') :: ['patients'] @entity11968 :: ('3082', 'Gene') :: ['hepatocyte growth factor', 'HGF'] @entity0 :: ('MESH:D001943', 'Disease') :: ['osteotropic breast cancer', 'breast cancer', 'increased breast cancer'] @entity5 :: ('MESH:D009369', 'Disease') :: ['osteotropic cancer', 'cancer', 'tumour'] @entity5298 :: ('8600', 'Gene') :: ['RANK ligand', 'RANKL']
@entity11968 :: (3082,Gene) :: ['hepatocyte growth factor']
