Poultry by-products are not often processed into high-value products. Rather than being transformed into meal for animal feed, a large quantity of @entity1573 skin could be used to produce collagen, which is valued for its unique functional properties. The purpose of this research project was to extract and characterize collagen from @entity1573 skin. Skins were first ground and then were heated to 40 or 60 degrees C to extract the fat. After mechanical separation, the collagen contained in the resulting solid phase was extracted with pepsin or @entity9373 . Types I and III collagen were then isolated and characterized by SDS PAGE, antigen labeling, determination of @entity669 residues, and transmission electron microscopy. The total collagen content of the skin was recovered from the solid phase following heat treatment at 40 degrees C. Extraction yields varied with the solubilization process: 38.9% of the collagen content in the solid phase was extracted with pepsin and 25.1% with @entity9373 . Ratios of type I to type III collagen fractionated using @entity1219 were 74.4:19.8% with pepsin and 62.4:31.7% with @entity9373 . Characterization tests further revealed the presence of telopeptides solely on @entity9373 -solubilized collagen. @entity1573 skin thus appears to be a good alternative source of high-quality collagen.

Extraction and characterization of collagen with or without telopeptides from XXXX skin.

@entity1573 :: ('9031', 'Species') :: ['chicken', 'Chicken'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine'] @entity1219 :: ('CHEBI:26710', 'Chemical') :: ['NaCl'] @entity9373 :: ('MESH:C031234', 'Chemical') :: ['ethylene diamine']
@entity1573 :: (9031,Species) :: ['chicken']
