Tissue @entity32 often results in peritoneal scar tissue formation. The objective of this study was to determine whether mesothelial cells which line the peritoneal cavity express matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs), and if their expression is regulated by @entity2533 , a key regulator of tissue @entity82 . For this purpose we used @entity1 cells, a cell line derived from @entity1 normal mesothelial cells, and for comparative analysis we used U-937 cells, a @entity1 monocytic/macrophage cell line. The cells were treated with @entity2533 (1 ng/ml) for various time periods and the levels of MMP and @entity2843 mRNA and protein expression were determined using quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. The results indicate that the mesothelial cells and macrophages express @entity3334 (collagenase-1), @entity1549 (stromelysin-1), @entity2843 and @entity1552 mRNA and protein at various levels, with significantly higher TIMPs than MMPs, and higher @entity3334 than @entity1549 (p < 0.001). The mesothelial cells express significantly less @entity3334 , higher @entity1549 and similar levels of @entity2843 mRNA compared to macrophages. In a time-dependent manner, treatment of the mesothelial cells with @entity2533 resulted in a significant decrease in the expression of @entity3334 , while increasing the expression of @entity2843 mRNA (p = 0.05). In contrast, @entity1549 and @entity1552 expression was unaffected in mesothelial cells and in macrophages, compared to untreated controls. There was a significant increase in secreted @entity3334 and @entity1552 by mesothelial cells following @entity2533 treatments in a time-dependent manner (p = 0.05 and p = 0.01), without affecting the secretion of these proteins by macrophages. A major portion of @entity3334 in the culture conditioned media of both cell types was found in complex with @entity2843 . The ratios of @entity3334 /TIMPs production were significantly higher than @entity1549 /TIMPs in mesothelial cells and macrophages, and progressively decreased following @entity2533 treatments (p < 0.05). In conclusion, these results indicate that mesothelial cells express MMP and @entity2843 mRNA and protein, and their expression is differentially regulated by @entity2533 , a mechanism that in part may influence the outcome of peritoneal tissue repair and adhesion formation.

Expression of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinases in mesothelial cells and their regulation by XXXX .

@entity1 :: ('9606', 'Species') :: ['human', 'Met-5A'] @entity32 :: ('MESH:D007249', 'Disease') :: ['injury and pelvic inflammation'] @entity1552 :: ('7077', 'Gene') :: ['TIMP-2'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis'] @entity3334 :: ('4312', 'Gene') :: ['MMP-1'] @entity1549 :: ('4314', 'Gene') :: ['MMP- 3', 'MMP-3'] @entity2843 :: ('7076', 'Gene') :: ['TIMP', 'TIMP-1'] @entity2533 :: ('7040', 'Gene') :: ['transforming growth factor-beta1']
@entity2533 :: (7040,Gene) :: ['transforming growth factor-beta1']
