Agrobacterium-mediated plant genetic transformation involves a complex interaction between the bacterium and the host plant. Relatively little is known about the role plant genes and proteins play in this process. We previously identified an @entity958 mutant, @entity4468 , that is resistant to Agrobacterium transformation. We show here that @entity4468 contains a T-DNA insertion into the 3'-untranslated region of the cellulose synthase-like gene @entity4468 . @entity4468 transcripts are greatly reduced in the @entity4468 mutant. Genetic complementation of @entity4468 with wild-type genomic copies of the @entity4468 gene restores both transformation competence and the wild-type level of @entity4468 transcripts. The @entity4468 promoter shows a distinct pattern of expression in @entity958 plants. In particular, the promoter is active in the elongation zone of roots, the root tissue that we previously showed is most susceptible to Agrobacterium-mediated transformation. Disruption of the @entity4468 gene in the @entity4468 mutant results in reduced numbers and rate of growth of lateral roots and reduced ability of the roots to bind A. tumefaciens cells under certain conditions. No major differences in the linkage structure of the non-cellulosic polysaccharides could be traced to the defective @entity4468 gene.

Agrobacterium-mediated root transformation is inhibited by mutation of an XXXX cellulose synthase-like gene.

@entity958 :: ('3702', 'Species') :: ['Arabidopsis'] @entity4468 :: ('831734(Tax:3702)', 'Gene') :: ['rat4', 'CSLA9']
@entity958 :: (3702,Species) :: ['Arabidopsis']
