Cord blood (CB) is increasingly used as a source of hematopoietic stem cells (HSC) for transplantation. Low incidence and severity of @entity2132 ( @entity2132 ) and a robust graft-versus- @entity1778 (GvL) effect are observed following CB transplantation (CBT). However, its main disadvantages are a limited number of HSC per unit, delayed immune reconstitution and a higher incidence of @entity281 . Unmanipulated grafts contain accessory cells that may facilitate HSC engraftment. Therefore, the effects of accessory cells, particularly natural killer (NK) cells, on @entity1 CB HSC (CBSC) functions were assessed in vitro and in vivo. CBSC cultured with autologous CB NK cells showed higher levels of @entity9843 expression, a higher migration index and a higher number of colony forming units (CFU) after short-term and long-term cultures. We found that CBSC secreted @entity12426 following interaction with CB NK cells. In addition, recombinant @entity12426 increased CBSC clonogenicity, recapitulating the effect observed of CB NK cells on CBSC. Moreover, the co-infusion of CBSC with CB NK cells led to a higher level of CBSC engraftment in NSG @entity19 model. The results presented in this work offer the basis for an alternative approach to enhance HSC engraftment that could improve the outcome of CBT.

Natural Killer Cells Improve Hematopoietic Stem Cell Engraftment by Increasing Stem Cell Clonogenicity In Vitro and in a Humanized XXXX Model.

@entity1 :: ('9606', 'Species') :: ['human'] @entity12426 :: ('17329(Tax:10090)', 'Gene') :: ['CXCL9'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity9843 :: ('12767(Tax:10090)', 'Gene') :: ['CXCR4'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity1778 :: ('MESH:D007938', 'Disease') :: ['leukemia'] @entity2132 :: ('MESH:D006086', 'Disease') :: ['GvHD', 'graft-versus-host disease']
@entity19 :: (10090,Species) :: ['Mouse']
