UNASSIGNED: Virus-like particles of @entity1082 -VLP), resulting from the self-assembly of the capsid proteins (L1 or L1 and L2), have been widely used to study HPV as they are similar to the native virion. Moreover, two prophylactic vaccines, @entity14849 (  ) and Cervarix(  ) , are based on HPV-VLP L1. Analytical techniques currently used to characterize HPV-VLP, such as @entity214 -PAGE, Western blot, ELISA, are time-consuming and semi-quantitative. In this study, capillary electrophoresis (CE) was evaluated for the analysis of intact @entity1799 -VLP. The usefulness of capillary inner wall coating as well as various BGEs, pH and detergent additives were investigated. Reproducible HPV-VLP analysis in CE was achieved using @entity968 coated capillary and a BGE containing high salt concentration and low @entity214 concentration. The developed method enables HPV-VLP detection in less than 10 min (migration times RSD : 1.6%). The identity of HPV-VLP peak was confirmed by comparison with a sample obtained from a wild-type baculovirus and with VLP-based vaccine, @entity14849 (  ) , after adjuvant dissolution. Finally, we applied the developed methodology to VLP-based vaccines, demonstrating that CE could be successfully used for vaccine quality control. This article is protected by copyright. All rights reserved.

Study of intact virus-like particles of XXXX by capillary electrophoresis.

@entity14849 :: ('MESH:C512720', 'Chemical') :: ['Gardasil'] @entity1799 :: ('333760', 'Species') :: ['HPV16'] @entity1082 :: ('10566', 'Species') :: ['human papillomavirus (HPV'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS'] @entity968 :: ('MESH:D011092', 'Chemical') :: ['poly(ethylene oxide)']
@entity1082 :: (10566,Species) :: ['human papillomavirus']
