Previous studies showed that the distribution of antigenic site specificity of neutralizing antibodies to type 3 @entity9925 obtained with the inactivated @entity9925 vaccine can be deficient as compared with that obtained following @entity9925 @entity281 . This observation was shown by the relatively low capacity of sera from inactivated- @entity9925 -vaccine-immunized @entity1 to neutralize @entity9925 cleaved at antigenic site 1. We investigated possibilities for improving the situation in a @entity19 model. Balb/c @entity19 were immunized with intact or trypsin-cleaved type 3 @entity9925 (Saukett strain). Sera from @entity19 immunized with the intact virus readily neutralized the intact virus but neutralized the cleaved virus only rarely. In contrast, cleaved-virus-immunized mice produced antibodies that were able to neutralize the cleaved virus as well as the intact one. @entity19 immunized with a 100-fold-higher dose of the intact virus produced significant levels of antibodies to the cleaved virus, too. Somewhat surprisingly, @entity19 immunized with high doses of the cleaved virus produced antibodies specific for the intact loop between beta sheets B and C of VP1 (virion protein 1), which should be cleaved in the immunogen. This was shown by a higher titer of antibodies to intact Saukett virus than to the corresponding cleaved virus, as well as to a type 1/type 3 hybrid @entity9925 in which only the BC loop @entity1522 were derived from type 3 @entity9925 . The cleavage-induced enhanced availability of antigenic determinants residing outside the BC loop was also shown by increased neutralization titers of monoclonal antibodies specific for some of these other determinants. These results indicate that by using a trypsin-cleaved type 3 @entity9925 as a parenteral immunogen, it is possible to change the distribution of antigenic site specificities of neutralizing antibodies to resemble that following @entity9925 @entity281 .

Improved distribution of antigenic site specificity of poliovirus-neutralizing antibodies induced by a protease-cleaved immunogen in XXXX .

@entity1 :: ('9606', 'Species') :: ['persons'] @entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity9925 :: ('138950', 'Species') :: ['poliovirus'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'Mice'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection']
@entity19 :: (10090,Species) :: ['mice']
