@entity5 stem cells (CSCs) are defined as a subset of slow cycling and undifferentiated cells that divide asymmetrically to generate highly proliferative, invasive, and chemoresistant @entity5 cells. Therefore, CSCs are an attractive population of cells to target therapeutically. CSCs are predicted to contribute to a number of types of @entity5 including those in the blood, brain, lung, gastrointestinal tract, prostate, and ovary. Isolating and enriching a @entity5 cell population for CSCs will enable researchers to study the properties, genetics, and therapeutic response of CSCs. We generated a protocol that reproducibly enriches for @entity43 CSCs from @entity43 cell lines (SKOV3 and OVCA429). Cell lines are treated with 20   M @entity900 for 3 days. Surviving cells are isolated and cultured in a serum-free stem cell media containing cytokines and growth factors. We demonstrate an enrichment of these purified CSCs by analyzing the isolated cells for known stem cell markers @entity4729 , @entity2440 , and @entity5439 ( @entity5439 ) and cell surface expression of @entity15392 and @entity5439 . The CSCs exhibit increased chemoresistance. This method for isolation of CSCs is a useful tool for studying the role of CSCs in chemoresistance and @entity5 relapse.

Enrichment for chemoresistant XXXX stem cells from human cell lines.

@entity43 :: ('MESH:D010051', 'Disease') :: ['ovarian cancer'] @entity4729 :: ('5460', 'Gene') :: ['Oct4'] @entity2440 :: ('79923', 'Gene') :: ['Nanog'] @entity5439 :: ('8842', 'Gene') :: ['Prom1', 'CD133'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'Cancer', 'malignancies'] @entity900 :: ('MESH:D002945', 'Chemical') :: ['cisplatin'] @entity15392 :: ('57126', 'Gene') :: ['CD177']
@entity43 :: (MESH:D010051,Disease) :: ['ovarian cancer']
