OBJECTIVES: 1) To determine if endothelial expression of adhesion molecules involved in leukocyte recruitment is increased in the brain and other organs in four different models of experimental @entity1960 , and 2) to investigate whether leukocyte infiltration occurs in the brain of colitic animals. METHODS: Endothelial @entity297 ( @entity297 ) and @entity1590 ( @entity1590 ) expression was quantified, using the dual radiolabeled antibody technique in @entity35 with @entity1959 ( @entity10776 )-induced @entity1960 , in @entity19 with dextran sulfate @entity1372 (DSS)-induced @entity1960 , in @entity915 @entity19 reconstituted with CD45RBhigh T-cells, and in @entity1618 -/- @entity19 . Leukocyte infiltration in the brain of TNBS-induced colitic @entity35 was assessed by myeloperoxidase activity and immunohistochemical staining with anti-CD45 monoclonal antibody. RESULTS: Marked upregulation of brain endothelial @entity297 (2- to 5.5-fold) was consistently found in colitic animals in the four models studied. Brain @entity297 strongly correlated with colon @entity297 and colon weight. By contrast, upregulation of brain @entity1590 in colitic animals was only observed in the CD45RBhigh transfer (3-fold) and the TNBS-induced (1.5-fold models). Heart and muscle @entity297 and @entity1590 were not upregulated in colitic animals in the majority of models studied. There was no leukocyte infiltration into the brain of TNBS-induced colitic @entity35 . CONCLUSIONS: Our study demonstrates a marked and specific upregulation of endothelial @entity297 in the brain of colitic animals. This activation of cerebral endothelial cells was not associated with an infiltration of leukocytes into brain tissue.

Brain endothelial adhesion molecule expression in experimental XXXX .

@entity297 :: ('25361(Tax:10116)', 'Gene') :: ['VCAM-1', 'vascular cell adhesion molecule-1'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity1372 :: ('MESH:D012964', 'Chemical') :: ['sodium'] @entity1618 :: ('16153(Tax:10090)', 'Gene') :: ['IL-10'] @entity1959 :: ('MESH:D014302', 'Chemical') :: ['trinitrobenzenesulfonic acid'] @entity1960 :: ('MESH:D003092', 'Disease') :: ['colitis'] @entity915 :: ('MESH:D053632', 'Disease') :: ['SCID'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity10776 :: ('CHEBI:53063', 'Chemical') :: ['TNBS'] @entity1590 :: ('25464(Tax:10116)', 'Gene') :: ['ICAM-1', 'intercellular adhesion molecule-1']
@entity1960 :: (MESH:D003092,Disease) :: ['colitis']
