The CC chemokine eotaxin is potent eosinophil-selective chemoattractant, and it is thought that the function of eotaxin is closely related to the recruitment of eosinophils in certain inflammatory reactions. In order to learn more about the biological role of this molecule, we have developed a new sandwich ELISA method to measure @entity1 eotaxin using two monoclonal anitibodies and purified recombinant eotaxin as a standard. The minimal detectable concentration of eotaxin in this assay was 1.5 pg/ml, and the working range was 3.1--200 pg/ml with low CVs (< 10%). Both within- and between-run precision levels were less than 6.7% of the CVs. The dilution curves of two serum and two spiked plasma samples showed good linearity and the recovery range was 92.8--103.3%. No cross-reactivity was found with other similar chemokines. @entity2842 , @entity12869 , @entity7721 , @entity12866 , @entity28955 and @entity5737 . This assay was sensitive enough to measure the circulating eotaxin levels of healthy volunteers. However, the eotaxin levels in serum samples (mean+/-SD; 68.6+/-13.4 pg/ml, n=15) were significantly higher than those in matched plasma samples (19.2+/-5.4 pg/ml) separated from blood collected in tubes containing @entity1098 . Kinetic studies revealed that the eotaxin levels in serum markedly increased depending on the elapsed time before separation from blood cells, but such changes in @entity1098 -plasma were negligible up to 4 h at 25 degrees C. Our new ELISA is an accurate and useful method for quantifying @entity1 eotaxin in blood and demonstrates that the process of preparing blood samples affects the measurement of the eotaxin levels.

Development of a sensitive enzyme-linked immunosorbent assay for eotaxin and measurement of its levels in XXXX blood.

@entity1 :: ('9606', 'Species') :: ['human'] @entity12869 :: ('6355', 'Gene') :: ['MCP-2'] @entity12866 :: ('6357', 'Gene') :: ['MCP-4'] @entity5737 :: ('6352', 'Gene') :: ['RANTES'] @entity7721 :: ('6354', 'Gene') :: ['MCP-3'] @entity2842 :: ('6347', 'Gene') :: ['MCP-1'] @entity28955 :: ('6369', 'Gene') :: ['eotaxin-2'] @entity1098 :: ('MESH:D004492', 'Chemical') :: ['EDTA']
@entity1 :: (9606,Species) :: ['human']
