The production of microRNAs (miRNA) is influenced by various stimuli, including environmental stresses. We hypothesized that reactive @entity26 species (ROS)-associated stress could regulate macrophage miRNA synthesis. miRNAs undergo unique steps of maturation processing through either one of two pathways of cytoplasmic processing. Unlike the canonical pathway, the regulation of alternative cytoplasmic processing of miRNA has not been fully elucidated yet. We cultured bone marrow derived macrophages (BMDM) from wild type (WT) and @entity14099 (phox-/-) @entity19 and profiled miRNA expression using microarrays. We analyzed 375 miRNAs including four endogenous controls to normalize the data. At resting state, @entity14099 (phox-/-) BMDM has the markedly reduced expression of @entity771 compared to WT BMDM, without other significant differences. Unlike majority of miRNAs, @entity771 goes through the unique alternative processing pathway, in which @entity46946 plays a key role. In spite of significant reduction of mature @entity771 , however, its precursor form, pre- @entity771 , was similar in both BMDMs, suggesting that the processing of pre- @entity771 is impaired in @entity14099 (phox-/-) BMDM. Moreover, @entity14099 (phox-/-) BMDM expressed significantly reduced level of @entity46946 . In contrast, @entity46946 mRNA levels were similar in WT and @entity14099 (phox-/-) BMDM, suggesting a post-transcriptional defect of @entity46946 production in @entity14099 (phox-/-) macrophages, which resulted in impaired processing of pre- @entity771 . In order to examine the functional significance of @entity771 in macrophages, we cultured BMDMs from @entity771 knock-out @entity19 . Of interest, @entity771 -deficient BMDM exhibited reduced ROS generation upon zymosan stimulation, compared to WT BMDM. Our studies suggest functional crosstalk between ROS and @entity771 in the regulation of macrophage oxidant stress.

@entity14099 phox and reactive XXXX species production modulate expression of microRNA-451 in macrophages.

@entity14099 :: ('386649(Tax:10090)', 'Gene') :: ['p47'] @entity46946 :: ('239528(Tax:10090)', 'Gene') :: ['Ago2'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity771 :: ('723870(Tax:10090)', 'Gene') :: ['mir-451', 'miR-451']
@entity26 :: (MESH:D010100,Chemical) :: ['oxygen']
