The authors have previously described a model of in vivo tissue generation based on an implanted, microsurgically created vessel loop in a plastic chamber (volume, 0.45 ml) containing a @entity7050 ( @entity7050 ) scaffold. Tissue grew spontaneously in association with an intense angiogenic sprouting from the loop and almost filled the chamber, resulting in a mean amount of tissue in chambers of 0.23 g with no added matrix scaffold and 0.33 g of tissue in @entity7050 -filled chambers after 4 weeks of incubation. The aim of the present study was to investigate whether a greater volume of tissue could be generated when the same-size vessel loop was inserted into a larger (1.9 ml) chamber. In four groups of five @entity35 , an arteriovenous shunt sandwiched between two disks of @entity7050 , used as a scaffold for structural support, was placed inside a large polycarbonate growth chamber. Tissue and @entity7050 weight and volume, as well as histological characteristics of the newly formed tissue, were assessed at 2, 4, 6, and 8 weeks. Tissue weight and volume showed a strong linear correlation. Tissue weight increased progressively from 0.13 +/- 0.04 g at 2 weeks to 0.57 +/- 0.06 g at 6 weeks (p < 0.0005). @entity7050 weight decreased progressively from 0.89 +/- 0.07 g at 2 weeks to 0.20 +/- 0.09 g at 8 weeks (p < 0.0005). Histological examination of the specimens confirmed increased tissue growth and maturation over time. It is concluded that larger quantities of tissue can be grown over a longer period of time by using larger-size growth chambers.

Increasing the volume of vascularized tissue formation in engineered constructs: an experimental study in XXXX .

@entity35 :: ('10116', 'Species') :: ['rats'] @entity7050 :: ('MESH:C043435', 'Chemical') :: ['poly(DL-lactic-co-glycolic acid)', 'PLGA']
@entity35 :: (10116,Species) :: ['rats']
