UNASSIGNED: The @entity29117 genome encodes homologues of divisome proteins including FtsZ and FtsA. FtsZ of this bacterium (Dr-FtsZ) has been recently characterized. In this paper, we study FtsA of @entity29117 (Dr-FtsA) and its involvement in regulation of FtsZ function. Recombinant Dr-FtsA showed neither ATPase nor GTPase activity and its polymerization was @entity855 dependent. Interestingly, we observed that Dr-FtsA, when compared with @entity526 FtsA (Ec-FtsA), has lower affinity for both Dr-FtsZ and Ec-FtsZ. Also, Dr-FtsA showed differential effects on GTPase activity and sedimentation characteristics of Dr-FtsZ and Ec-FtsZ. For instance, Dr-FtsA stimulated GTPase activity of Dr-FtsZ while GTPase activity of Ec-FtsZ was reduced in the presence of Dr-FtsA. Stimulation of GTPase activity of Dr-FtsZ by Dr-FtsA resulted in depolymerization of Dr-FtsZ. Dr-FtsA effects on GTPase activity and polymerization/depolymerisation characteristics of Dr-FtsZ did not change significantly in the presence of @entity855 . Recombinant @entity526 expressing Dr-FtsA showed cell division inhibition in spite of in trans expression of Dr-FtsZ in these cells. These results suggested that Dr-FtsA, although it lacks ATPase activity, is still functional and differentially affects Dr-FtsZ and Ec-FtsZ function in vitro.

Dr-FtsA, an Actin Homologue in XXXX Differentially Affects Dr-FtsZ and Ec-FtsZ Functions In Vitro.

@entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP'] @entity29117 :: ('1299', 'Species') :: ['D. radiodurans', 'Deinococcus radiodurans'] @entity526 :: ('562', 'Species') :: ['E. coli']
@entity29117 :: (1299,Species) :: ['Deinococcus radiodurans']
