UNASSIGNED: @entity68687 (HGA) is the toxic principle in ackee (Blighia sapida Koenig), a nutritious and readily available fruit which is a staple of the Jamaican working-class and rural population. The aril of the unripe fruit has high concentrations of HGA, the cause of Jamaican @entity391 , which is very often fatal. HGA is also present in the samara of several species of maple (Acer spp.) which are suspected to cause seasonal pasture @entity121 in North America and @entity1641 @entity121 in Europe, often fatal for @entity1641 . The aim of this study was to develop a method for quantifying HGA in blood that would be sensitive enough to provide toxicological evidence of ackee or maple poisoning. Analysis was carried out using solid-phase extraction (HILIC cartridges), @entity11521 derivatization and UHPLC-HRMS/MS detection. The method was validated in whole blood with a detection limit of 0.35 g/L (range: 0.8-500 g/L). This is the first method applicable in forensic toxicology for quantifying HGA in whole blood. HGA was quantified in two serum samples from @entity1641 suffering from @entity121 . The concentrations were 446.9 and 87.8 g/L. HGA was also quantified in dried arils of unripe ackee fruit (Suriname) and seeds of sycamore maple (Acer pseudoplatanus L.) (France). The concentrations were 7.2 and 0.74mg/g respectively.

A validated method for quantifying XXXX in whole blood by UHPLC-HRMS/MS.

@entity1641 :: ('9796', 'Species') :: ['equine', 'horses'] @entity391 :: ('MESH:D014839', 'Disease') :: ['vomiting sickness'] @entity68687 :: ('MESH:C041757', 'Chemical') :: ['Hypoglycin A'] @entity121 :: ('MESH:D009135', 'Disease') :: ['myopathy', 'atypical myopathy'] @entity11521 :: ('MESH:C496906', 'Chemical') :: ['dansyl']
@entity68687 :: (MESH:C041757,Chemical) :: ['hypoglycin A']
