BACKGROUND: Left and right @entity694 can display different clinical, pathologic, and genetic characteristics. The purpose of this study was to characterize multiple molecular genetic alterations in sporadic @entity694 and to correlate them with the location of the @entity5 and with lymph node @entity3 . METHODS: One hundred and twenty-five cases of sporadic @entity694 (50 in the right colon and 75 in the left colon in @entity1 with no family history of @entity694 ) were studied. Status of the @entity876 gene was assessed by polymerase chain reaction (PCR), single strand conformation polymorphism, and sequencing at exons 5-8. @entity124 was analyzed with five microsatellite markers at chromosome 18. The mismatch repair genes @entity6756 and @entity4311 were studied in @entity5 found to have microsatellite instability by PCR and sequencing. RESULTS: In the 125 cases studied, there were 53 @entity5 with mutations of the @entity876 gene. @entity876 mutations correlated with lymph node @entity3 from right @entity694 cases (61%), and all cases with @entity876 mutations and microsatellite instability were AJCC/UICC Stage III (Dukes Stage C). In the right @entity694 cases the rate of microsatellite instability was related to the @entity5 size (19% in @entity5 measuring < 4 cm, and 34% in @entity5 measuring > 4 cm). No correlation between microsatellite instability and @entity876 mutations was detected. In the left @entity694 cases, @entity876 mutations were detected in 41% of @entity5 and microsatellite instability in 14%; neither finding was related to the @entity5 size. Mutations of the @entity6756 and @entity4311 mismatch repair genes were detected in 7 of 24 cases with marked microsatellite instability. CONCLUSIONS: @entity124 is prone to occur in sporadic right @entity694 during @entity5 growth and is not associated significantly with mutations in the @entity6756 and @entity4311 mismatch repair genes or in the @entity876 gene. Concomitant detection of microsatellite instability and @entity876 mutations in right @entity694 is associated with the presence of lymph node @entity3 .

@entity124 and @entity876 mutations in sporadic right and left XXXX : different clinical and molecular implications.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity6756 :: ('4292', 'Gene') :: ['hMLH1'] @entity124 :: ('MESH:D053842', 'Disease') :: ['Microsatellite instability'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity4311 :: ('4436', 'Gene') :: ['hMSH2'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis', 'metastases'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon carcinomas', 'colon carcinoma']
@entity694 :: (MESH:D003110,Disease) :: ['colon carcinoma']
