AIMS: To investigate effect of metallic nanoparticles, @entity575 (AgNPs) and gold nanoparticles (AuNPs) as antitumor treatment in vitro against @entity1 @entity0 cells (MCF-7) and their associated mechanisms. This could provide new class of engineered nanoparticles with desired physicochemical properties and may present newer approaches for therapeutic modalities to @entity0 in @entity1 . MATERIALS AND METHODS: A @entity1 @entity0 cell line (MCF-7) was used as a model of cells. Metallic nanoparticles were characterized using UV-visible spectra and transmission electron microscopy (TEM). Cytotoxic effects of metallic nanoparticles on MCF-7 cells were followed by colorimetric SRB cell viability assays, microscopy, and cellular uptake. Nature of cell death was further investigated by DNA analysis and flow cytometry. RESULTS: Treatment of MCF-7 with different concentrations of 5-10nm diameter of AgNPs inhibited cell viability in a dose-dependent manner, with IC50 value of 6.28 M, whereas treatment of MCF-7 with different concentrations of 13-15nm diameter of AuNPs inhibited cell viability in a dose-dependent manner, with IC50 value of 14.48 M. Treatment of cells with a IC50 concentration of AgNPs generated progressive accumulation of cells in the S phase of the cell cycle and prevented entry into the M phase. The treatment of cells with IC50 concentrations of AuNPs similarly generated progressive accumulation of cells in sub-G1 and S phase, and inhibited the entrance of cells into the M phase of the cell cycle. DNA fragmentation, as demonstrated by electrophoresis, indicated induction of apoptosis. CONCLUSIONS: Our engineered @entity575 nanoparticles effectively inhibit the proliferation of @entity1 @entity0 cell line MCF-7 in vitro at high concentration (1000  M) through apoptotic mechanisms, and may be a beneficial agent against @entity1 @entity189 but further detailed study is still needed.

Anti-proliferative Activities of Metallic Nanoparticles in an in Vitro XXXX .

@entity1 :: ('9606', 'Species') :: ['women', 'human'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinoma'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast carcinoma', 'breast cancer'] @entity575 :: ('MESH:D012834', 'Chemical') :: ['silver']
@entity0 :: (MESH:D001943,Disease) :: ['Breast Cancer Model']
