@entity3252 is one of the most common infections all over the world. Most cases are asymptomatic, except in immunosuppressed individuals and fetuses, which can be seriously damaged. Prenatal diagnosis should be made as soon as possible since treatment of the mother can minimize fetal sequelae. Our aim in this study was to test the polymerase chain reaction technique (PCR) in 86 samples of amniotic fluid from @entity1 who seroconverted during pregnancy. DNA was amplified using external primers and, in a second step, internal primers, in a nested PCR system. Samples were also inoculated into @entity19 and the newborn were evaluated by @entity3204 serology, skull x-ray, transfontanel ultrasound, fundoscopic examination, lumbar puncture and clinical examination. PCR was positive in seven cases and negative in 79. Among PCR-positive cases, two were negative by inoculation into @entity19 and by clinical evaluation; among PCR-negative ones, three had clinical evidence of @entity3252 and one was positive after inoculation into @entity19 . PCR showed values of sensitivity = 62.5% and specificity = 97.4%; the values of inoculation into @entity19 where 42.9% and 100%, respectively. Although PCR should not be used alone for prenatal diagnosis of @entity3251 , it is a promising method and deserves more studies to improve its efficacy.

Prenatal XXXX diagnosis from amniotic fluid by PCR.

@entity1 :: ('9606', 'Species') :: ['women'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity3252 :: ('MESH:D014123', 'Disease') :: ['toxoplasmosis', 'Toxoplasmosis'] @entity3251 :: ('MESH:D014125', 'Disease') :: ['congenital toxoplasmosis'] @entity3204 :: ('5811', 'Species') :: ['T. gondii']
@entity3252 :: (MESH:D014123,Disease) :: ['toxoplasmosis']
