INTRODUCTION: @entity2858 near full-length genome ( @entity645 -NFLG) sequencing from plasma is an attractive multidimensional tool to apply in large-scale population-based molecular epidemiological studies. It also enables genotypic resistance testing (GRT) for all drug target sites allowing effective intervention strategies for control and prevention in high-risk population groups. Thus, the main objective of this study was to develop a simplified subtype-independent, cost- and labour-efficient @entity645 -NFLG protocol that can be used in clinical management as well as in molecular epidemiological studies. METHODS: Plasma samples (n=30) were obtained from @entity2858 B (n=10), @entity2858 C (n=10), CRF01_AE (n=5) and CRF01_AG (n=5) infected individuals with minimum viral load >1120 copies/ml. The amplification was performed with two large amplicons of 5.5 kb and 3.7 kb, sequenced with 17 primers to obtain @entity645 -NFLG. GRT was validated against ViroSeq    @entity2858 Genotyping System. RESULTS: After excluding four plasma samples with low-quality RNA, a total of 26 samples were attempted. Among them, NFLG was obtained from 24 (92%) samples with the lowest viral load being 3000 copies/ml. High (>99%) concordance was observed between @entity645 -NFLG and ViroSeq    when determining the drug resistance mutations (DRMs). The @entity69371 connection mutation was additionally detected by NFLG in two samples. CONCLUSIONS: Our high efficiency subtype-independent @entity645 -NFLG is a simple and promising approach to be used in large-scale molecular epidemiological studies. It will facilitate the understanding of the @entity2858 pandemic population dynamics and outline effective intervention strategies. Furthermore, it can potentially be applicable in clinical management of drug resistance by evaluating DRMs against all available antiretrovirals in a single assay.

Subtype-independent near full-length XXXX genome sequencing and assembly to be used in large molecular epidemiological studies and clinical management.

@entity2858 :: ('11676', 'Species') :: ['HIV-1'] @entity645 :: ('57667', 'Species') :: ['HIV'] @entity69371 :: ('p|SUB|N|384|I', 'ProteinMutation') :: ['N384I']
@entity2858 :: (11676,Species) :: ['HIV-1']
