We describe an automated assay for @entity1420 (P4) in human serum and plasma with the Abbott AxSYM random-access immunoassay analyzer. In this one-step competitive assay, P4 immobilized onto latex microparticles competes with sample P4 for binding to a conjugate of alkaline phosphatase (AP) and anti-P4 antibody. Total CVs ranged from 3.4% to 8.2% in multiple precision studies conducted according to the 20-day NCCLS EP5-T protocol. The detection limit (zero calibrator + 2 SD) was 0.10 microg/L across 36 experiments. Values for diluted samples were 83-116% of expected. Recovery of P4 added to serum specimens was 92-115%. Cross-reactivities with 43 natural and synthetic @entity230 were 0-6.3%. No significant interference was detected from @entity648 , protein, erythrocytes, hemoglobin, @entity1674 , or @entity165 . In a multisite correlation study, AxSYM P4 results compared well with results from a commercial RIA method (n = 1156; r = 0.976; slope = 1.03; y-intercept = 0.04). Assay throughput is >80 tests per hour in batch mode, 60 tests per hour with mixed load list configurations.

Rapid, automated assay for XXXX on the Abbott AxSYM analyzer.

@entity1420 :: ('MESH:D011374', 'Chemical') :: ['progesterone'] @entity648 :: ('MESH:D001663', 'Chemical') :: ['bilirubin'] @entity1674 :: ('MESH:D014280', 'Chemical') :: ['triglycerides'] @entity230 :: ('MESH:D013256', 'Chemical') :: ['steroids'] @entity165 :: ('MESH:D002784', 'Chemical') :: ['cholesterol']
@entity1420 :: (MESH:D011374,Chemical) :: ['progesterone']
