PURPOSE: Transcription factors regulating the epithelial-to-mesenchymal transition (EMT) program contribute to @entity105 and @entity3 in many @entity5 , including cutaneous @entity320 . However, little is known about the role of EMT factors in the growth and metastatic dissemination of @entity2184 cells. Here, we analyzed the expression and functions of the EMT factors @entity18075 , @entity8945 , and @entity4245 in @entity2184 cell lines and @entity5 . METHODS: @entity18075 , @entity8945 , and @entity4245 mRNA levels were measured using qPCR in five @entity2184 cell lines and in 30 @entity5 . Gene expression was used to determine class 1 and class 2 signatures in the @entity5 . Short hairpin RNA was used to downregulate the expressions of the EMT factors; then, growth and transwell invasion assays were performed. RESULTS: @entity18075 , @entity8945 , and @entity4245 were expressed in all five @entity2184 lines, with @entity18075 having the highest protein levels. @entity18075 mRNA was significantly elevated in highly metastatic class 2 @entity5 for which survival data were not available, whereas a high gene expression of @entity8945 was associated with a worse prognosis in a separate @entity5 cohort analyzed by expression profiling. The genetic downregulation of @entity18075 in @entity27521 , OMM1, and 92.1 resulted in a more than 50% reduction in invasion, but only suppressed growth in OMM1 cells. Suppression of @entity8945 in Mel290 and OMM1 reduced growth and invasion by more than 50%. The downregulation of @entity4245 in the 92.1 cell line reduced invasion by 50%, but did not interfere with growth. CONCLUSIONS: The downregulation of @entity18075 , @entity8945 , and @entity4245 reduces the invasive properties of @entity2184 cells, and the elevated mRNA levels of @entity18075 and @entity8945 are associated with a more @entity146 clinical phenotype in @entity2184 samples. Therefore, these factors could represent new therapeutic targets in @entity1 with @entity320 .

EMT-associated factors promote invasive properties of XXXX cells.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity4245 :: ('6615', 'Gene') :: ['Snail1'] @entity146 :: ('MESH:D001523', 'Disease') :: ['aggressive'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'primary tumors', 'tumors'] @entity18075 :: ('6935', 'Gene') :: ['ZEB1'] @entity2184 :: ('MESH:C536494', 'Disease') :: ['uveal melanoma'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity8945 :: ('7291', 'Gene') :: ['Twist1'] @entity105 :: ('MESH:D063646', 'Disease') :: ['carcinogenesis'] @entity27521 :: ('83984', 'Species') :: ['OCM1'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma', 'ocular melanoma']
@entity2184 :: (MESH:C536494,Disease) :: ['uveal melanoma']
