OBJECTIVE: To put the insight into the @entity4965 ( @entity4966 )-induced effect on the differential expression of subcellular proteins in @entity1 normal liver cell line (L-02). METHODS: The membrane proteins and nuclear proteins of @entity4966 -treated (8.0 mmol/L) group and controls were extracted by subcellular proteome extraction kit, respectively. The @entity4966 -induced differentially expressions were analyzed by a two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization tandem time-of-flight spectrometry (MALDI-TOF-MS). Bioinformatics analysis was used to reveal the biological processes and predict transmembrane domains of differential expressed proteins. The expression of @entity855 synthase subunit beta ( @entity68093 ), @entity68094 ( @entity68094 ) and far up steam element-binding protein 1 ( @entity68095 ) were measured under @entity4966 treatment by Western blot. RESULTS: After @entity4966 treatment for 24 h in L-02 cells, 14 membrane proteins and 18 nuclear proteins were identified as differential expression. After treated with @entity4966 in concentrations of 0, 2.0, 4.0 and 8.0 mmol/L for 24 h, the relative levels of @entity68093 expression were 1.00  0.03, 1.21  0.14, 1.25  0.12 and 1.48  0.17 (F = 8.51, P = 0.007), the relative levels of @entity68094 expression were 1.00  0.09, 1.22  0.15, 1.43  0.21, 1.53  0.17 (F = 6.57, P = 0.015), respectively; the relative levels of @entity68095 expression were 1.00  0.11, 0.91  0.07, 0.73  0.04 and 0.67  0.03 (F = 15.81, P = 0.001), respectively, which were consistent with the results in proteomics. The bioinformatics analysis showed that the most dominant biological process were involved in RNA processing (10 proteins, P = 2.46*10(-6)), especially in RNA splicing (9 proteins, P = 1.77*10(-7)). CONCLUSION: The exposure of @entity4966 could alter the expression of membrane proteins and nuclear proteins in L-02 cells. These abnormal expressed proteins involved in RNA splicing would provide novel clues for further understanding of @entity4966 -induced hepatotoxicity.

[Investigation of XXXX -induced effects on subcellular proteomes in L-02 hepatic cells].

@entity1 :: ('9606', 'Species') :: ['human'] @entity68093 :: ('506', 'Gene') :: ['ATP5B'] @entity4966 :: ('CHEBI:16602', 'Chemical') :: ['TCE'] @entity68095 :: ('8880', 'Gene') :: ['FUBP1'] @entity68094 :: ('3188', 'Gene') :: ['heterogeneous nuclear ribonucleoprotein H2', 'hnRNP H2'] @entity4965 :: ('MESH:D014241', 'Chemical') :: ['trichloroethylene'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity4965 :: (MESH:D014241,Chemical) :: ['trichloroethylene']
