PURPOSE: To investigate the changes in Tenon's capsule fibroblast (HTF) proliferation and their expressions of @entity2533 ( @entity2533 ) and matrix metalloproteinases (MMPs) in @entity1 with long-term use of topical antiglaucoma medications, and to evaluate the potential effect of these medications on the outcome of @entity726 filtering surgery for @entity725 ( @entity725 ). METHODS: HTFs isolated from the conjunctiva of ten @entity725 @entity1 and ten control @entity1 with @entity231 or @entity1713 were tested for their in vitro proliferation capacity. RT-PCR (reverse-transcription polymerase chain reaction) was performed to detect the expressions of @entity2533 and MMP mRNAs in the HTFs, and the expression of @entity6474 protein was detected immunohistochemically. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of @entity2533 in the supernatant of the HTFs treated with the topical antiglaucoma agents. RESULTS: The HTFs from the @entity725 @entity1 showed significantly higher proliferative activity than those from the control @entity1 (P   =   0.0013). The HTFs in the @entity725 group showed significantly higher expressions of @entity6474 and @entity1934 mRNA and lower @entity1495 mRNA than the control HTFs. @entity1549 mRNA was not detected in the HTFs in @entity725 groups, but lowly expressed in the control HTFs. The HTFs in the @entity725 group had a significantly higher expression of @entity6474 protein; @entity2533 protein was not detected in the control HTFs, but was highly expressed in the @entity725 group. Treatment of the HTFs with latanoprost resulted in a 4-fold increase in @entity2533 expression in both groups, which was comparable for the expression after the exposure. CONCLUSIONS: Long-term use of some topical antiglaucoma medications might be associated with enhanced HTF proliferation and abnormal expressions of @entity2533 and MMPs, which might have promoted scarring of the filtering @entity4530 following @entity726 filtering surgery.

Long-term topical antiglaucoma medications cause enhanced Tenon's capsule fibroblast proliferation and abnormal @entity2533 and MMP expressions: potential effects on XXXX filtering surgery.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity725 :: ('MESH:C562750', 'Disease') :: ['primary open-angle glaucoma', 'POAG'] @entity231 :: ('MESH:D002386', 'Disease') :: ['cataract'] @entity4530 :: ('MESH:D001768', 'Disease') :: ['blebs'] @entity1713 :: ('MESH:D012163', 'Disease') :: ['retinal detachment'] @entity726 :: ('MESH:D005901', 'Disease') :: ['glaucoma'] @entity1549 :: ('4314', 'Gene') :: ['MMP-3'] @entity2533 :: ('7040', 'Gene') :: ['TGF-b', 'transforming growth factor-b', 'TGF-b1'] @entity1934 :: ('7043', 'Gene') :: ['TGF-b3'] @entity1495 :: ('4318', 'Gene') :: ['MMP-9'] @entity6474 :: ('7042', 'Gene') :: ['TGF-b2']
@entity726 :: (MESH:D005901,Disease) :: ['glaucoma']
