UNASSIGNED: In @entity14 ( @entity14 ), an increase in the stromal (S) area with the reduction of the epithelial (E) parts has been suggested as an indication of @entity5 progression. Therefore, an automated image method capable of discriminating E and S areas would allow an improved diagnosis. Immunofluorescence staining was performed on @entity982 -embedded sections from @entity14 (16 samples from @entity1 with liver @entity3 and 18 without). @entity5 adjacent mucosa (n = 5) and normal mucosa (n = 4) were taken as controls. Epithelial cells were identified by an anti-keratin 8 ( @entity10585 ) antibody. Large tissue areas (5-63   mm(2)/slide) including @entity5 center, @entity5 front, and adjacent mucosa were scanned using an automated microscopy system (TissueFAXS). With our newly developed algorithms, we showed that there is more @entity10585 -immunoreactive E in the @entity5 center than in @entity5 adjacent and normal mucosa. Comparing @entity1 with and without @entity3 , the E/S ratio decreased by 20% in the @entity5 center and by 40% at @entity5 front in metastatic samples. The reduction of E might be due to a more @entity146 phenotype in @entity3 @entity1 . The novel software allowed a detailed morphometric analysis of @entity5 tissue compartments as tools for objective quantitative measurements, reduced analysis time, and increased reproducibility of the data.

Quantitative Image Analysis of Epithelial and Stromal Area in Histological Sections of XXXX : An Emerging Diagnostic Tool.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity10585 :: ('3856', 'Gene') :: ['K8'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'Noncancerous tumor'] @entity146 :: ('MESH:D001523', 'Disease') :: ['aggressive'] @entity982 :: ('MESH:D010232', 'Chemical') :: ['paraffin'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity14 :: ('MESH:D015179', 'Disease') :: ['CRC', 'colorectal tumors', 'colorectal cancer']
@entity14 :: (MESH:D015179,Disease) :: ['Colorectal Cancer']
