OBJECTIVE: To explore the reaction of normal skin fibroblasts from different sites of @entity1 body to cyclic stretch. METHODS: The normal skin tissues from scapular upper back and medial side of upper arm of 3 @entity1 were cultured in vitro. Fibroblasts of experimental group were loaded by cyclic stretch with 10% amplitude for 24, 36, and 48 hours respectively. Fibroblasts of control group were cultured without cyclic stretch. The morphologic changes were observed using inverted microscope. CCK-8 method was used to detect the proliferation of the fibroblasts. The expressions of @entity2393 mRNA, @entity20336 ( @entity20336 ) mRNA, @entity2533 ( @entity2533 ) mRNA, and @entity13239 chain ( @entity13239 ) mRNA were detected by real-time quantitative PCR. The protein levels of collagen type I and @entity2533 were detected by ELISA. RESULTS: The cultured cells showed a significantly increased cell proliferation ability, and apparent orientation after the applied strain. The proliferation activity, mRNA expression levels of @entity2393 , @entity20336 , and @entity2533 , protein levels of @entity2533 in back skin were significantly higher than those in arm skin (P<0.05) when the fibroblasts were loaded for 36 and 48 hours, but no significant difference between back skin and arm skin at 24 hours (P>0.05). There was no significant difference in mRNA expression level of @entity13239 and protein level of collagen type I between back skin and arm skin at 24, 36, and 48 hours (P>0.05). There was no significant difference in all above indexes between back skin and arm skin in control group (P>0.05). CONCLUSION: Fibroblasts from scapular upper back and medial side of upper arm display different reactions to cyclic stretch, which indicates that there exists site difference in the reactions of fibroblasts to cyclic stretch. It might be related with the incidence of @entity2805 in different sites of the body.

[REACTION OF XXXX FIBROBLASTS FROM DIFFERENT SITES TO THE MECHANICAL STRESS].

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity13239 :: ('1277', 'Gene') :: ['collagen type I alpha1', 'COL1A1'] @entity2533 :: ('7040', 'Gene') :: ['TGF-beta1', 'transform growth factor beta1'] @entity2393 :: ('3688', 'Gene') :: ['integrin beta1'] @entity2805 :: ('MESH:D017439', 'Disease') :: ['hypertrophic scar'] @entity20336 :: ('9564', 'Gene') :: ['P130Cas', 'p130Crk-associated substance']
@entity1 :: (9606,Species) :: ['HUMAN']
