UNASSIGNED: @entity6621 is the prototypic poxvirus. The 192 kilobase double-stranded DNA viral genome encodes most if not all of the viral replication machinery. The @entity6621 DNA polymerase is encoded by the @entity69276 gene. Sequence analysis indicates that E9 is a member of the B family of replicative polymerases. The enzyme has both polymerase and 3'-5' exonuclease activities, both of which are essential to support viral replication. Genetic analysis of E9 has identified residues and motifs whose alteration can confer temperature-sensitivity, drug resistance ( @entity17720 , @entity7091 , @entity5538 arabinsode, @entity7076 ) or altered fidelity. The polymerase is involved both in DNA replication and in recombination. Although inherently distributive, E9 gains processivity by interacting in a 1:1 stoichiometry with a heterodimer of the @entity31907 and D4 proteins. @entity31907 binds to both @entity41666 and serves as a bridge within the holoenzyme. The @entity31907 /D4 heterodimer has been purified and can confer processivity on purified E9. The interaction of @entity31907 with D4 is mediated by the N'-terminus of @entity31907 . The D4 protein is an enzymatically active @entity5537 DNA glycosylase. The DNA-scanning activity of D4 is proposed to keep the holoenzyme tethered to the DNA template but allow polymerase translocation. The crystal structure of D4, alone and in complex with A201-50 and/or DNA has been solved. Screens for low molecular weight compounds that interrupt the A201-50/D4 interface have yielded hits that disrupt processive DNA synthesis in vitro and/or inhibit plaque formation. The observation that an active DNA repair enzyme is an integral part of the holoenzyme suggests that DNA replication and repair may be coupled.

The XXXX DNA polymerase and its processivity factor.

@entity17720 :: ('MESH:D010746', 'Chemical') :: ['phosphonoacetic acid'] @entity7091 :: ('MESH:D016590', 'Chemical') :: ['aphidicolin'] @entity69276 :: ('3707598(Tax:10245)', 'Gene') :: ['E9L'] @entity41666 :: ('397', 'Gene') :: ['E9 and D4'] @entity5538 :: ('MESH:D003596', 'Chemical') :: ['cytosine'] @entity31907 :: ('28935', 'Gene') :: ['A20'] @entity7076 :: ('MESH:C059262', 'Chemical') :: ['cidofovir'] @entity5537 :: ('MESH:D014498', 'Chemical') :: ['uracil'] @entity6621 :: ('10245', 'Species') :: ['Vaccinia virus', 'vaccinia virus']
@entity6621 :: (10245,Species) :: ['vaccinia virus']
