The study of @entity235 bone marrow-derived mesenchymal stem cells (MSCs) has a prominent position in veterinary cell-based applications. Yet the plethora of breeds, their different life spans, and interbreed variations provide unclearness on what can be achieved specifically by such therapies. In this study, we compared a set of morphological, physiological, and genetic markers of MSCs derived from large @entity235 breeds, namely, Border collie, German shepherd, Labrador, Malinois, Golden retriever, and Hovawart. We compared colony-forming units (CFUs) assay, population doubling time (PDT), senescence-associated b-galactosidase (SA-b-gal) activity, telomere length, and gene expression of MSCs, as well as the ability of cells to differentiate to osteogenic, adipogenic, and chondrogenic phenotypes. The influence of the culture media a-MEM, low- @entity413 DMEM, and high- @entity413 DMEM, used in cell isolation and expansion, was investigated in the presence and absence of @entity24976 ( @entity24976 ). Initial cell yield was not affected by culturing medium, but MSCs expanded best in a-MEM supplemented with @entity24976 . After isolation, the number of MSCs was similar among breeds--as shown by equivalent CFUs--except in the Hovawart samples, which had fivefold less CFU. Telomere lengths were similar among breeds. MSCs divided actively only for 4 weeks in culture (PDT   =      50 h/division), except Border collie cells divided for a longer time than cells from other groups. The percentage of senescent cells increased linearly in all breeds with time, with a faster rate in German shepherd, Labrador, and Golden retriever. Border collie cells underwent efficient osteogenic differentiation, Hovawart cells performed the best in chondrogenic differentiation, and Labrador cells in both, while German shepherd cells had the lower differentiation potential. MSCs from all breeds preserved the same adipogenic differentiation potential. In conclusion, despite variations, isolated MSCs can be expanded and differentiated in vitro, and all breeds are eligible for MSC-based therapies.

XXXX Mesenchymal Stem Cell Potential and the Importance of @entity235 Breed: Implication for Cell-Based Therapies.

@entity235 :: ('9615', 'Species') :: ['canine', 'dog'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity24976 :: ('403857(Tax:9615)', 'Gene') :: ['basic fibroblast growth factor', 'bFGF']
@entity235 :: (9615,Species) :: ['Canine']
