1. A method is described for the @entity969 -gradient sedimentation analysis of ribosomes in a post-mitochondrial supernatant of @entity35 skeletal muscle. 2. An essential feature of the method involves the use of buffer of ionic strength 0.3 for homogenization of the muscle tissue. 3. Polyribosomes can be prepared by precipitation from post-mitochondrial supernatant of skeletal muscle by adjustment of the @entity4337 content of the medium. These polyribosomes stimulate cell-free @entity535 incorporation in vitro in an energy-dependent system. 4. Ribosome aggregates of uniform size distribution can be obtained by adjustment of the ionic strength of the post-mitochondrial supernatant, followed by differential sucrose-gradient centrifugation. 5. In vivo, @entity35 skeletal-muscle polyribosomes became labelled by @entity3209 -labelled @entity535 within 15min., and radioactivity was associated with the light ribosome species within 45min. 6. Electron microscopy of the polyribosomes revealed aggregations containing more than 40 single ribosomes.

Preparation and some properties of XXXX skeletal-muscle polyribosomes.

@entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity3209 :: ('CHEBI:36927', 'Chemical') :: ['(14)C'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity4337 :: ('MESH:D011189', 'Chemical') :: ['potassium chloride']
@entity35 :: (10116,Species) :: ['rat']
