The N @entity19 oligodendroglial cell line, immortalized with SV40 T antigen, simultaneously expresses oligodendroglial markers and @entity9327 ( @entity9327 ), an astroglial marker. This study examines the plasticity of N @entity19 cells with regard to @entity9327 expression, and its relationship to expression of SV40 T antigen, @entity899 , and a novel nuclear antigen detected by the A007 monoclonal antibody. Marked changes occur in @entity9327 levels and cell morphology when N @entity19 cells are switched from the permissive temperature (34 degrees C) to the non-permissive temperature (39 degrees C), and with @entity1919 elevation at 39 degrees C. At 34 degrees C, levels of @entity9327 are high; when cells are switched to 39 degrees C, @entity9327 levels decrease significantly, then increase slightly when @entity1928 is added. At both temperatures, the cells display feathery @entity9327 immunostaining. When @entity1928 is added at 39 degrees C, however, cells display bright fibrous @entity9327 staining in elongated processes. The changes in @entity9327 were compared to changes in T antigen and @entity899 . As expected, the decrease in T antigen at 39 degrees C was accompanied by movement of @entity899 from the nucleus to cytoplasm. Total @entity899 levels did not change, however, and @entity1928 did not alter the respective distribution or levels of @entity899 at either temperature. At both temperatures, the cell bodies and processes show internal expression of sulfatide, as demonstrated with the O4, Sulph I, and A007 antibodies. We show, for the first time, abundant nuclear immunoreactivity with the A007 monoclonal antibody in the N @entity19 cells. This nuclear reactivity is seen at 34 degrees C, but not at 39 degrees C, similar to @entity899 , and is not detected with the other @entity10839 antibodies. Double-label immunostaining shows that the nuclear A007 immunoreactivity is co-localized in nuclear structures with T antigen and @entity899 at 34 degrees C, but is not found in every nucleus containing these antigens. We conclude that regulation of @entity9327 expression and morphology in N @entity19 cells is dependent on a combination of T antigen expression and level of @entity268 and may be related to regulation of @entity899 and the A007 nuclear antigen.

Regulation of CNS glial phenotypes in N XXXX cells.

@entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity9327 :: ('14580(Tax:10090)', 'Gene') :: ['glial fibrillary acidic protein', 'GFAP'] @entity19 :: ('10090', 'Species') :: ['20.1'] @entity899 :: ('22060(Tax:10090)', 'Gene') :: ['p53'] @entity1919 :: ('MESH:D000242', 'Chemical') :: ['cyclic AMP'] @entity10839 :: ('CHEBI:18318', 'Chemical') :: ['sulfatide'] @entity1928 :: ('MESH:D005576', 'Chemical') :: ['forskolin']
@entity19 :: (10090,Species) :: ['20.1']
