4(a-l-Rhamnosyloxy)-benzyl isothiocyanate (glucomoringin isothiocyanate; GMG-ITC) is released from the precursor 4(a-l-rhamnosyloxy)-benzyl glucosinolate (glucomoringin; GMG) by myrosinase (b-thioglucoside glucohydrolase; E.C. 3.2.1.147) catalyzed hydrolysis. GMG is an uncommon member of the glucosinolate group as it presents a unique characteristic consisting in a second glycosidic residue within the side chain. It is a typical glucosinolate found in large amounts in the seeds of Moringa oleifera Lam., the most widely distributed plant of the Moringaceae family. GMG was purified from seed-cake of M. oleifera and was hydrolyzed by myrosinase at neutral pH in order to form the corresponding GMG-ITC. This bioactive phytochemical can play a key role in counteracting the inflammatory response connected to the oxidative-related mechanisms as well as in the control of the neuronal cell death process, preserving spinal cord tissues after injury in @entity19 . @entity1566 was induced in @entity19 by the application of vascular clips (force of 24g) for 1min., via four-level T5-T8 after laminectomy. In particular, the purpose of this study was to investigate the dynamic changes occurring in the spinal cord after ip treatment with bioactive GMG-ITC produced 15min before use from myrosinase-catalyzed hydrolysis of GMG (10mg/kg body weight+5 l Myr @entity19 /day). The following parameters, such as histological damage, distribution of reticular fibers in connective tissue, nuclear factor (NF)-kB translocation and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha ( @entity10181 -a) degradation, expression of inducible Nitric Oxide Synthases (iNOS), as well as apoptosis, were evaluated. In conclusion, our results show a protective effect of bioactive GMG-ITC on the secondary damage, following @entity1566 , through an antioxidant mechanism of neuroprotection. Therefore, the bioactive phytochemical GMG-ITC freshly produced before use by myrosinase-catalyzed hydrolysis of pure GMG, could prove to be useful in the treatment of @entity1566 .

4(a-l-Rhamnosyloxy)-benzyl isothiocyanate, a bioactive phytochemical that attenuates secondary damage in an experimental model of XXXX .

@entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity1566 :: ('MESH:D013119', 'Disease') :: ['Spinal cord trauma', 'spinal cord injury', 'spinal cord trauma'] @entity10181 :: ('18036(Tax:10090)', 'Gene') :: ['IkB']
@entity1566 :: (MESH:D013119,Disease) :: ['spinal cord injury']
