PURPOSE: To examine the effects of @entity899 status and @entity2307 treatment on potentially lethal damage repair, referring to the response of intratumor quiescent cells. METHODS: @entity1 head and @entity213 cells transfected with mutant @entity876 (SAS/mp53) or with neo vector as a control (SAS/neo) were injected subcutaneously into both hind legs of Balb/cA @entity19 . @entity19 bearing the @entity5 received @entity1603 ( @entity1603 ) continuously to label all proliferating (P) cells in the @entity5 . The @entity19 then received gamma-rays with or without subsequent @entity2307 administration. Right after or 24 h after gamma-ray irradiation alone or 24 h after @entity2307 administration following irradiation, the @entity5 were excised, minced, and trypsinized. The @entity5 cell suspensions thus obtained were incubated with a cytokinesis blocker (cytochalasin-B), and the micronucleus (MN) frequency in cells without @entity1603 labeling [quiescent (Q) cells] was determined using immunofluorescence staining for @entity1603 . The MN frequency in total (P+Q) @entity5 cells was determined from the @entity5 that were not pretreated with @entity1603 . RESULTS: On the whole, larger values of MN frequency and surviving fraction were observed in SAS/mp53 cells than in SAS/neo cells, and Q cells showed lower MN frequencies than total cells. Without @entity2307 , SAS/neo @entity5 cells, especially Q cells within SAS/neo @entity5 , showed large potentially lethal damage repair (PLDR) capacities, compared with total or Q @entity5 cells within SAS/mp53 @entity5 that showed little PLDR capacity. @entity2307 treatment inhibited the PLDR in SAS/neo @entity5 very effectively, but showed no apparent effect on either total or Q @entity5 cells within SAS/mp53 @entity5 . CONCLUSION: PLDR in vivo was thought to be a @entity899 -dependent event whether in total or Q @entity5 cell populations.

Effects of @entity899 status and XXXX treatment on potentially lethal damage repair, with emphasis on the response of intratumor quiescent cells.

@entity1 :: ('9606', 'Species') :: ['Human'] @entity1603 :: ('MESH:D001973', 'Chemical') :: ['BrdU', "5-bromo-2'-deoxyuridine"] @entity899 :: ('22060(Tax:10090)', 'Gene') :: ['p53'] @entity19 :: ('10090', 'Species') :: ['mice', 'nude mice', 'Mice'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity2307 :: ('MESH:C009687', 'Chemical') :: ['wortmannin', 'Wortmannin'] @entity876 :: ('7157', 'Gene') :: ['TP53'] @entity213 :: ('MESH:C535575', 'Disease') :: ['neck squamous cell carcinoma']
@entity2307 :: (MESH:C009687,Chemical) :: ['wortmannin']
