BACKGROUND: Although glacial @entity1543 ( @entity12190 ) treatment of bloody cervical samples has reduced the rate of unsatisfactory Pap Tests, recent studies suggest that it may negatively impact high-risk (hr)-HPV test results. The objectives of this study were to compare the levels of genomic DNA between @entity12190 treated and nontreated ThinPrep(  ) samples using the Hologic(  ) Cervista(  ) HPV-HR assay and to compare the adequacy of the ThinPrep(  ) Pap Test between aliquoted and nonaliquoted samples. METHODS: Prior to @entity12190 treatment, 2.5 ml of the cervical sample was prealiquoted from 102 bloody ThinPrep(  ) vials. Both @entity12190 treated and nontreated samples were analyzed for hr-HPV using the Cervista(  ) HPV HR assay. The levels of genomic DNA were measured and compared between these samples. In addition, ThinPrep(  ) Pap Test adequacy rates were calculated and compared on aliquoted and nonaliquoted cervical samples. RESULTS: Of the 102 cervical samples, 95 (93%) nontreated aliquots contained satisfactory levels of genomic DNA as compared to 36 (35%) @entity12190 -treated samples (p   <   0.00001). Ninety-nine (97%) aliquoted cervical samples were satisfactory for cytologic evaluation as compared to 1,326 (96%) @entity12190 treated samples from 2013 (nonaliquoted); not statistically significant (p   =   0.7968). CONCLUSION: The levels of genomic DNA were significantly decreased in @entity12190 treated vs non-treated TP samples. Aliquoting from the TP sample prior to treatment with @entity12190 enables accurate measurement of DNA without affecting the adequacy of the TP cytology slide. Diagn. Cytopathol. 2016.    2016 Wiley Periodicals, Inc.

Maximizing the adequacy of Hologic(  ) Cervista(  ) HPV HR results on ThinPrep(  ) Pap samples treated with glacial XXXX .

@entity12190 :: ('2548', 'Gene') :: ['GAA'] @entity1543 :: ('MESH:D019342', 'Chemical') :: ['acetic acid']
@entity1543 :: (MESH:D019342,Chemical) :: ['acetic acid']
