Hematopoietic cell transplantation (HCT) is the only cure for @entity3458 ( @entity3458 ), but engraftment remains challenging in @entity1 lacking matched donors. Infusion of mesenchymal stromal cells (MSCs) at the time of HCT may promote @entity2132 . Experimental @entity19 models suggest MSC major histocompatibility complex compatibility with recipient impacts their in vivo function, suggesting autologous MSCs could be superior to third-party MSCs for promoting HCT engraftment. Here we tested whether bone marrow (BM)-derived MSCs from @entity3458 subjects have comparable functionality compared with MSCs from healthy volunteers. @entity3458 MSC doubling time and surface marker phenotype did not differ significantly from non- @entity3458 . Third-party and autologous ( @entity3458 ) T cell proliferation was suppressed in a dose-dependent manner by all MSCs. @entity3458 MSCs comparably expressed @entity3035 -2,3-dioxygenase, which based on transwell and blocking experiments appeared to be the dominant immunomodulatory pathway. The expression of key genes involved in hematopoietic stem cell (HSC)-MSC interactions was minimally altered between @entity3458 and non- @entity3458 MSCs. Expression was, however, altered by IFN-y stimulation, particularly @entity15874 , CXCL26, @entity17184 , CKITL, and @entity127 , indicating the potential to augment MSC expression by cytokine stimulation. These data demonstrate the feasibility of expanding BM-derived MSCs from @entity3458 @entity1 that phenotypically and functionally do not differ per International Society of Cell Therapy essential criteria from non- @entity3458 MSCs, supporting initial evaluation (primarily for safety) of autologous MSCs to enhance haploidentical HSC engraftment in @entity3458 .

Bone Marrow-Derived Mesenchymal Stromal Cells from XXXX with Sickle Cell Disease Display Intact Functionality.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity3035 :: ('CHEBI:28626', 'Chemical') :: ['indoleamine'] @entity15874 :: ('9547', 'Gene') :: ['CXCL14'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity3458 :: ('MESH:D000755', 'Disease') :: ['SCD', 'sickle cell disease'] @entity2132 :: ('MESH:D006086', 'Disease') :: ['hematopoiesis and ameliorate graft-versus-host disease'] @entity17184 :: ('6376', 'Gene') :: ['CX3CL1'] @entity127 :: ('182', 'Gene') :: ['JAG1']
@entity1 :: (9606,Species) :: ['Patients']
