OBJECTIVE: To get initial experience with alternative sampling (self-sampling) for HPV testing as the means of @entity5 screening program. DESIGN: Original work. SETTING: Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University in Olomouc. METHODS: Based on expression of interest, 215 self-sampling kits were posted to @entity1 . Evalyn   Brush Vaginal swabs obtained by self-sampling were analyzed for the presence of @entity281 by Cobas 4800 HPV (Roche) followed by genotyping using PapilloCheck   HPV-Screening (Greiner Bio-One). Sixty @entity1 randomly chosen from our sample were sent a questionnaire focused on their experience with self-sampling. RESULTS: One hundred seventy-four of 215 (81%) distributed self-sampling devices have been delivered to analysis. All cervicovaginal swabs were sampled correctly and it was possible to analyze them by Cobas 4800 HPV test. Similarly, 98% (171/174) samples were analyzable by PapilloCheck   HPV-Screening.One hundred twenty-five (72%) of 174 tested samples were HPV negative. Low risk @entity281 was detected only in 7 samples (4%), and high risk HPV (hrHPV) @entity281 was present in 42 samples (24%). The most frequently detected hrHPV genotypes were @entity1799 (11/42; 26%) and HPV53 (6/42; 14%). @entity325 was detected in 10 cases, in 5 of them lrHPV @entity281 was find also.Of the 60 questionnaires, 48 (80%) were returned. From this group, 47 (98%) @entity1 rated their experience with self-sampling device as good to excellent. User manual of self-sampling device was considered good to excellent by all @entity1 (100%). All @entity1 also rated the convenience of self-sampling device using as good to excellent. As expected, most of the @entity1 (n = 42 [88%]) preferred self-sampling to physician sampling. CONCLUSION: Cervicovaginal self-sampling leads to valid results of HPV screening using two molecular genetics methods and was accepted by Czech @entity1 very well. The self-sampling as an opportunity to participate in @entity5 screening could increase the attendance of the screening program and would help to reduce the incidence and mortality for this disease in the Czech population.

[Utilization of self-sampling kits for HPV testing in  XXXX screening - pilot study].

@entity1 :: ('9606', 'Species') :: ['women'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cervical cancer'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection', 'HPV infection'] @entity1799 :: ('333760', 'Species') :: ['HPV16'] @entity325 :: ('MESH:D060085', 'Disease') :: ['HrHPV co-infection']
@entity5 :: (MESH:D009369,Disease) :: ['cervical cancer']
