AIMS: The aim of this study was to determine whether @entity6756 inactivation precedes the progression of @entity75 ( @entity75 ) into @entity75 with cytologic @entity1141 ( @entity75 /D) and to define the histologic stage at which promoter methylation and inactivation of @entity6756 occur. METHODS AND RESULTS: By using the MassARRAY EpiTYPER assay and immunohistochemistry, we examined methylation levels and the protein expression status of @entity6756 in 33 @entity75 /Ds with @entity5115 and compared the results with those of control @entity1127 ( @entity1127 ) and SSLs. The methylation level of @entity6756 was higher in the @entity2483 of @entity75 /Ds (P = 0.005), and it significantly differed with regard to the degree of @entity1141 (P = 0.002). The methylation levels of @entity6756 in the @entity6349 component of @entity75 /Ds tended to be higher than those of control SSLs and @entity1127 (P = 0.063 and P = 0.017, respectively). The loss of @entity6756 protein expression was identified only in 13 of 33 (39.39%) @entity6349 components of @entity75 /Ds. CONCLUSION: Promoter methylation and loss of protein expression of @entity6756 are not parallel processes that occur concurrently. @entity6756 methylation is an early molecular event which occurs even in @entity4306 . However, the loss of @entity6756 expression is a much later step, found in approximately 40% of @entity75 /Ds at various histologic stages. Notably, the loss of @entity6756 protein expression does not necessarily precede the development of cytologic @entity1141 in @entity75 . This article is protected by copyright. All rights reserved.

Reappraisal of XXXX promoter methylation and protein expression status in the serrated neoplasia pathway.

@entity6756 :: ('4292', 'Gene') :: ['hMLH1'] @entity5115 :: ('MESH:C567703', 'Disease') :: ['conventional epithelial dysplasia'] @entity1127 :: ('MESH:D011127', 'Disease') :: ['HPs', 'hyperplastic polyps'] @entity75 :: ('MESH:D001927', 'Disease') :: ['SSL', 'sessile serrated lesion'] @entity4306 :: ('MESH:C537262', 'Disease') :: ['HP'] @entity2483 :: ('MESH:C566443', 'Disease') :: ['dysplastic component than in the non-dysplastic component'] @entity6349 :: ('MESH:D004416', 'Disease') :: ['dysplastic'] @entity1141 :: ('MESH:C535662', 'Disease') :: ['dysplasia']
@entity6756 :: (4292,Gene) :: ['hMLH1']
