Platelets play critical roles in hemostasis and @entity715 through their aggregation following activation of integrin alphaIIbbeta3. However, the molecular mechanism of the integrin activation inside platelets remains largely unknown. Pharmacological experiments have demonstrated that @entity7131 ( @entity7131 ) plays an important role in @entity1423 . Because @entity7131 inhibitors can have multiple substrates and given that non- @entity7131 - @entity4621 -binding signaling molecules have been demonstrated to play important roles, the precise involvement of @entity7131 in cellular functions requires re-evaluation. Here, we have established an assay for analyzing the @entity141 -induced aggregation of permeabilized platelets. The aggregation of platelets was inhibited by the addition of the @entity369 - @entity2228 - @entity492 - @entity563 peptide, an integrin-binding peptide inhibitor of alphaIIbbeta3, suggesting that the aggregation was mediated by the integrin. The aggregation was also dependent on exogenous @entity855 and platelet cytosol, indicating the existence of essential cytosolic factors required for the aggregation. To examine the role of @entity7131 in the aggregation assay, we immunodepleted @entity10185 and beta from the cytosol. The @entity7131 -depleted cytosol lost the aggregation-supporting activity, which was recovered by the addition of purified @entity10185 . Furthermore, the addition of purified @entity10185 in the absence of cytosol did not support the aggregation, whereas the cytosol containing less @entity7131 supported it efficiently, suggesting that additional factors besides @entity7131 would also be required. Thus, we directly demonstrated that @entity10185 is involved in the regulation of @entity141 -induced @entity1423 .

Direct demonstration of involvement of protein kinase Calpha in the XXXX -induced @entity1423 .

@entity2228 :: ('CHEBI:57305', 'Chemical') :: ['glycine'] @entity1423 :: ('MESH:D001791', 'Disease') :: ['platelet aggregation'] @entity7131 :: ('MESH:D020151', 'Disease') :: ['protein kinase C', 'PKC'] @entity4621 :: ('CHEBI:37532', 'Chemical') :: ['phorbol ester'] @entity492 :: ('CHEBI:29995', 'Chemical') :: ['aspartate'] @entity563 :: ('CHEBI:17822', 'Chemical') :: ['serine'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+'] @entity715 :: ('MESH:D013927', 'Disease') :: ['thrombosis'] @entity369 :: ('CHEBI:29016', 'Chemical') :: ['arginine'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP'] @entity10185 :: ('5578', 'Gene') :: ['PKCalpha']
@entity141 :: (CHEBI:29108,Chemical) :: ['Ca2+']
