OBJECTIVES: Diagnosis of @entity25443 ( @entity25443 ) remains challenging, especially in @entity468 without effusions. The objective of this study was to evaluate the sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction (RT-PCR) detecting @entity25444 (FCoV) RNA in peripheral blood mononuclear cells (PBMC) and serum in comparison with the same real-time RT-PCR in cell-free body cavity effusion. METHODS: This prospective case-control study included 92 @entity468 . Forty-three @entity468 had a definitive diagnosis of @entity25443 , established either by histopathological examination (n = 28) or by positive immunofluorescence staining of FCoV antigen in macrophages of effusions (n = 11), or by both methods (n = 4). Forty-nine control @entity468 had other diseases but similar clinical signs. Real-time RT-PCR was performed on PBMC of 37 @entity468 (21 @entity468 with @entity25443 , 16 controls), on serum of 51 @entity468 (26 @entity468 with @entity25443 , 25 controls) and on cell-free body cavity effusion of 69 @entity468 (36 @entity468 with @entity25443 , 33 controls). Sensitivity, specificity, positive and negative predictive value, including 95% confidence intervals (CI) were calculated. RESULTS: Real-time RT-PCR of PBMC, serum and cell-free body cavity effusion showed a specificity of 100% (95% CI 79.4-100% in PBMC, 86.3-100% in serum, 89.4-100% in cell-free body cavity effusion) and a sensitivity of 28.6% (95% CI 11.3-52.2%) in PBMC, 15.4% (95% CI 4.4-34.9%) in serum and 88.9% (95% CI 73.9-96.9%) in cell-free body cavity effusion to diagnose @entity25443 . CONCLUSIONS AND RELEVANCE: Although it is known that RT-PCR can often provide false positive results in healthy @entity468 , this real-time RT-PCR was shown to be a specific tool for the diagnosis of @entity25443 when applied in a clinical setting. Sensitivity in cell-free body cavity effusion was high but low in PBMC and serum. PBMC samples showed a higher sensitivity than serum samples, and is therefore a better choice if no effusion is present.

Comparison of real-time reverse transcriptase polymerase chain reaction of peripheral blood mononuclear cells, serum and cell-free body cavity effusion for the diagnosis of XXXX .

@entity25443 :: ('MESH:D016766', 'Disease') :: ['feline infectious peritonitis', 'FIP'] @entity468 :: ('9685', 'Species') :: ['cats'] @entity25444 :: ('12663', 'Species') :: ['feline coronavirus']
@entity25443 :: (MESH:D016766,Disease) :: ['feline infectious peritonitis']
