BACKGROUND: Brucella is a facultative, intracellular pathogen that causes severe disease in animals and @entity1 . Immunity against Brucella involves both humoral and cellular responses. To investigate the characteristics of immune response in @entity697 in Sprague-Dawley (SD) rats, IgG and its subclass specific immunoglobulins' (IgG1 and @entity17993 ) response in sera against @entity6469 biotype 1 infection were studied. METHODOLOGY: Thirty-six @entity35 were inoculated intraperitoneally with 0.1 ml apyrogenic saline containing 1x10(10) colony forming unit (CFU) of @entity6469 biotype 1 Korean @entity291 isolate. Four @entity35 were used as uninfected controls. The sera were collected from infected @entity35 at 3, 7, 14, 21, 28, 35, 42, 49, and 56 days post infection (DPI) and screened for Brucella specific antibody response by the rose bengal plate test (RBPT). IgG and its subclass specific immunoglobulins' (IgG1 and @entity17993 ) response in the sera were measured by a lipopolysaccharide (LPS) based indirect enzyme-linked immunosorbent assay (IELISA). RESULTS: Brucella specific IgG, IgG1 and @entity17993 responses in the sera of infected @entity35 were detected from 3 DPI by IELISA. IgG and IgG1 concentrations in sera reached the peak level at 35 DPI, and then the concentrations gradually declined to the end of the experiment. @entity17993 concentrations in the sera remained almost constant from 7 DPI until the end of this study. CONCLUSION: In @entity697 , @entity17993 response (indicative of a Th1 response) was found to be significantly dominant over IgG1 response (indicative of Th2 response) (P < 0.001).

Characteristics of the immune response during XXXX in @entity35 .

@entity1 :: ('9606', 'Species') :: ['humans'] @entity697 :: ('MESH:D000208', 'Disease') :: ['acute brucellosis'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity6469 :: ('235', 'Species') :: ['B. abortus'] @entity17993 :: ('367586(Tax:10116)', 'Gene') :: ['IgG2a'] @entity291 :: ('9913', 'Species') :: ['bovine']
@entity697 :: (MESH:D000208,Disease) :: ['acute brucellosis']
