Microbeam radiation treatment (MRT) using synchrotron radiation has shown great promise in the treatment of @entity1313 , with a demonstrated ability to eradicate the @entity5 while sparing normal tissue in small animal models. With the goal of expediting the advancement of MRT research beyond the limited number of synchrotron facilities in the world, we recently developed a compact laboratory-scale microbeam irradiator using @entity505 nanotube (CNT) field emission-based X-ray source array technology. The focus of this study is to evaluate the effects of the microbeam radiation generated by this compact irradiator in terms of @entity5 control and normal tissue damage in a @entity19 @entity1313 model. @entity19 with U87MG @entity1 @entity463 were treated with sham irradiation, low-dose MRT, high-dose MRT or 10 Gy broad-beam radiation treatment (BRT). The microbeams were 280  m wide and spaced at 900  m center-to-center with peak dose at either 48 Gy (low-dose MRT) or 72 Gy (high-dose MRT). Survival studies showed that the @entity19 treated with both MRT protocols had a significantly extended life span compared to the untreated control group (31.4 and 48.5% of life extension for low- and high-dose MRT, respectively) and had similar survival to the BRT group. Immunostaining on MRT @entity19 demonstrated much higher DNA damage and apoptosis level in @entity5 tissue compared to the normal brain tissue. Apoptosis in normal tissue was significantly lower in the low-dose MRT group compared to that in the BRT group at 48 h postirradiation. Interestingly, there was a significantly higher level of cell proliferation in the MRT-treated normal tissue compared to that in the BRT-treated @entity19 , indicating rapid normal tissue repairing process after MRT. Microbeam radiation exposure on normal brain tissue causes little apoptosis and no macrophage infiltration at 30 days after exposure. This study is the first biological assessment on MRT effects using the compact CNT-based irradiator. It provides an alternative technology that can enable widespread MRT research on mechanistic studies using a preclinical model, as well as further translational research towards clinical applications.

Treating XXXX with Microbeam Radiation Generated by a Compact @entity505 -Nanotube-Based Irradiator: Initial Radiation Efficacy Study.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1313 :: ('MESH:D001932', 'Disease') :: ['brain tumor', 'brain tumors'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'Mice'] @entity505 :: ('MESH:D002244', 'Chemical') :: ['carbon'] @entity463 :: ('MESH:D005909', 'Disease') :: ['glioblastoma']
@entity1313 :: (MESH:D001932,Disease) :: ['Brain Tumor']
