To study the mechanisms contributing to the recruitment of a selective leukocyte subset in @entity32 involving the airways as may occur in @entity565 , we examined whether @entity56 exposure induces the expression of cell adhesion molecules (CAMs) on the bronchial endothelium of passively sensitized @entity1 bronchi. @entity1 bronchial tissue obtained from @entity1 undergoing @entity2 surgery was passively sensitized with serum from @entity1 with @entity12131 who were sensitive to house dust mite. We incubated the tissues for 30, 120, 240, and 480 min in the presence or absence of the dust mite allergen. The tissues were stained immunohistochemically for @entity2071 ( @entity2071 ), @entity4105 , and @entity4725 ( @entity4725 ). @entity2071 was constitutively expressed in both the epithelium and endothelium in all tissues but after allergen stimulation significantly increased at 240 and 480 min. @entity4105 expression also existed constitutively and increased significantly at 120 and 240 min with allergen exposure. The constitutive expression of @entity4725 was less than that of @entity2071 and @entity4105 . Following allergen exposure, @entity4725 expression increased significantly at 30, 120, 240, and 480 min, and at 480 min reached an almost 3.5-fold increase from baseline expression. The @entity310 level in the supernatants significantly increased at 120 min after allergen stimulation, and the @entity1356 level increased in 4 of 15 samples. We also examined the induction of CAMs by @entity310 , @entity1356 , and @entity3646 on @entity1 bronchial tissue. @entity310 and @entity1356 increased the expression of @entity2071 , @entity4105 , and @entity4725 , whereas @entity3646 induced only that of @entity4725 . In addition, neutralizing antibody against @entity310 and @entity1356 partially blocked the upregulation of CAMs on passively sensitized bronchial tissue after allergen exposure. Thus, both an IgE-dependent @entity56 response and selected cytokines are able to upregulate endothelial CAMs in @entity1 bronchial tissue. These observations provide further evidence that leukocyte infiltration into the site of @entity32 as occurs in @entity12131 is in part regulated by the expression of @entity2071 , @entity4725 , and @entity4105 .

Allergen exposure induces the expression of endothelial adhesion molecules in passively sensitized XXXX bronchus: time course and the role of cytokines.

@entity1 :: ('9606', 'Species') :: ['human', 'patients', 'Human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['allergic inflammation'] @entity12131 :: ('MESH:C565292', 'Disease') :: ['atopic asthma'] @entity4725 :: ('7412', 'Gene') :: ['vascular cell adhesion molecule 1', 'VCAM-1'] @entity1356 :: ('3553', 'Gene') :: ['IL-1beta', 'interleukin (IL)-1beta'] @entity310 :: ('7124', 'Gene') :: ['TNF-alpha'] @entity565 :: ('MESH:D001249', 'Disease') :: ['asthma'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung cancer'] @entity4105 :: ('6401', 'Gene') :: ['E-selectin'] @entity56 :: ('MESH:D004342', 'Disease') :: ['allergic'] @entity2071 :: ('3383', 'Gene') :: ['ICAM-1', 'intercellular adhesion molecule 1'] @entity3646 :: ('3565', 'Gene') :: ['IL-4']
@entity1 :: (9606,Species) :: ['human']
