BACKGROUND: @entity2440 is a group-I bio-carcinogen for @entity201 ( @entity201 ). Although the epidemiological evidence links @entity14007 and @entity201 , the underlying molecular mechanism involved in this process is poorly understood. In the present study, we investigated expression of oncogenes and @entity5 suppressors, including @entity69631 , @entity69632 , p53 and @entity995 in @entity2441 induced hamster @entity201 model. METHODS: Different histochemical/immunohistochemical techniques were performed to detect @entity201 in 4 groups of hamsters: uninfected control (Ctrl.), infected with @entity2441 (Cs), ingested @entity1232 ( @entity8576 ), and both Cs infected and @entity8576 introduced (Cs+ @entity8576 ). The liver tissues from all groups were analyzed for gene/protein expressions by quantitative PCR (qPCR) and western blotting. PRINCIPAL FINDINGS: @entity201 was observed in all hamsters of Cs+ @entity8576 group with well, moderate, and poorly differentiated types measured in 21.8%    1.5%, 13.3%    1.3%, and 10.8%    1.3% of total tissue section areas respectively. All @entity201 differentiations progressed in a time dependent manner, starting from the 8th week of @entity281 . @entity201 stroma was characterized with increased collagen type I, mucin, and proliferative cell nuclear antigen (PCNA). The qPCR analysis showed @entity69631 , @entity69632 and p16INK4 were over-expressed, whereas p53 was under-expressed in the Cs+ @entity8576 group. We observed no change in RB1 at mRNA level but found significant down-regulation of @entity995 protein. The apoptosis related genes, BAX and caspase 9 were found downregulated in the @entity201 tissue. Gene/protein expressions were matched well with the pathological changes of different groups except the @entity8576 group. Though the hamsters in the @entity8576 group showed no marked pathological lesions, we observed over-expression of Akt/PKB and p53 genes proposing molecular interplay in this group which might be related to the @entity201 initiation in this animal model. CONCLUSIONS/SIGNIFICANCE: The present findings suggest that oncogenes, @entity69631 and @entity69632 , and @entity5 suppressors, p53 and @entity995 , are involved in the carcinogenesis process of @entity2441 induced @entity201 in hamsters.

Involvement of @entity69631 , @entity69632 , and @entity5 Suppressors in Development of Intrahepatic Cholangiocarcinoma of Syrian Golden Hamsters Induced by XXXX and @entity1232 .

@entity69632 :: ('101839492', 'Gene') :: ['CDK4'] @entity8576 :: ('CHEBI:59990', 'Chemical') :: ['NDMA'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity69631 :: ('101839602', 'Gene') :: ['PSMD10'] @entity14007 :: ('MESH:D003003', 'Disease') :: ['clonorchiasis'] @entity2441 :: ('143401', 'Species') :: ['C. sinensis'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity201 :: ('MESH:D018281', 'Disease') :: ['cholangiocarcinoma', 'CCA'] @entity2440 :: ('79923', 'Species') :: ['Clonorchis sinensis'] @entity1232 :: ('MESH:D004128', 'Chemical') :: ['N-nitrosodimethylamine'] @entity995 :: ('MESH:D012175', 'Disease') :: ['RB']
@entity2440 :: (79923,Species) :: ['Clonorchis sinensis']
