UNASSIGNED: In the @entity19 brain, the first post-mitotic cortical neurons formed during embryogenesis express store-operated channels (SOCs) sensitive to Pyr3, initially proposed as a blocker of the transient receptor potential channel of C type 3 ( @entity27175 channel). However, Pyr3 does not discriminate between Orai and @entity27175 channels, questioning the contribution of @entity27175 in SOCs. This study was undertaken to clarify the molecular identity and the pharmacological profile of native SOCs from E13 cortical neurons. The mRNA expression of STIM1-2 and @entity17832 -3 was assessed by quantitative reverse transcription polymerase chain reaction. E13 cortical neurons expressed STIM1-2 mRNAs, with @entity31749 being the predominant isoform. Only transcripts of @entity57413 were found but no @entity17832 and @entity57414 mRNAs. Blockers of Orai and TRPC channels (Pyr6, Pyr10, @entity69031 , SAR7334, and GSK-7975A) were used to further characterize the endogenous SOCs. Their activity was recorded using the fluorescent @entity141 probe @entity20048 . Cortical SOCs were sensitive to the Orai blockers Pyr6 and GSK-7975A, as well as to @entity69031 , @entity219 , @entity920 , and @entity2287 ions, the latter one being the most potent SOCs blocker tested (IC50    10 nM). SOCs were insensitive to the TRPC channel blockers Pyr10 and SAR7334. In addition, preventing mitochondrial @entity141 uptake inhibited SOCs which were unaffected by inhibitors of the @entity141 -independent phospholipase A2. Altogether, @entity57413 channels are present at the beginning of the embryonic @entity19 cortico-genesis and form the core component of native SOCs in the immature cortex. This @entity141 route is likely to play a role in the formation of the brain cortex.

Pharmacological Characterization of the Native Store-Operated Calcium Channels of Cortical Neurons from Embryonic XXXX Brain.

@entity57414 :: ('269999(Tax:10090)', 'Gene') :: ['Orai3'] @entity57413 :: ('269717(Tax:10090)', 'Gene') :: ['Orai2'] @entity69031 :: ('MESH:C562064', 'Chemical') :: ['EVP4593'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity27175 :: ('22065(Tax:10090)', 'Gene') :: ['TRPC3'] @entity17832 :: ('109305(Tax:10090)', 'Gene') :: ['Orai1'] @entity219 :: ('MESH:D015032', 'Chemical') :: ['zinc'] @entity920 :: ('MESH:D003300', 'Chemical') :: ['copper'] @entity31749 :: ('116873(Tax:10090)', 'Gene') :: ['STIM2'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity2287 :: ('MESH:D005682', 'Chemical') :: ['gadolinium'] @entity20048 :: ('MESH:C409648', 'Chemical') :: ['Fluo-4']
@entity19 :: (10090,Species) :: ['Mouse']
