Biofilms are microbial sessile communities attached to surfaces that are known for causing many medical problems. A bacterial biofilm of clinical relevance is formed by the gram-negative bacteria @entity796 . During the formation of a biofilm, the initial adhesion of the cells is of crucial importance, and the characteristics of the contact surface have great influence on this step. In the present study, we aimed to use matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI- @entity215 MS) profiling as a new methodology to monitor @entity796 biofilm development. Biofilms were grown within @entity7502 tubes containing a glass slide, and were harvested after 3, 5, 7, 9, or 12 days of inoculation. Planktonic cells were obtained separately by centrifugation as control. Two independent MALDI- @entity215 experiments were performed, one by collecting biofilms from both the glass slide and the @entity7502 tube internal surface, and the other by acquiring biofilms from these surfaces separately. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) were used to evaluate the morphological progression of the biofilm. The molecular results showed that MALDI profiling is able not only to distinguish between different biofilm stages, but it is also appropriate to indicate when the biofilm cells are released at the dispersion stage, which occurred first on @entity7502 surface. Finally, the present study pointed out that MALDI profiling may emerge as a promising tool for the clinical diagnostic and prognostic workup of biofilms formation and control.

Use of MALDI- XXXX mass spectrometry to analyze the molecular profile of @entity796 biofilms grown on glass and plastic surfaces.

@entity215 :: ('MESH:D013771', 'Disease') :: ['TOF'] @entity796 :: ('287', 'Species') :: ['Pseudomonas aeruginosa', 'P. aeruginosa'] @entity7502 :: ('MESH:D011126', 'Chemical') :: ['polypropylene']
@entity215 :: (MESH:D013771,Disease) :: ['TOF']
