OBJECTIVE: To investigate the effects of in utero exposure to high-dose @entity15523 ( @entity11559 ) on testicular cell apoptosis in late embryonic and pubertal male @entity35 offspring. METHODS: Twenty pregnant Sprague-Dawley (SD) @entity35 were divided into two groups. During gestation day (GD) 12 to GD 19, control group was given 1 ml day(-1) of olive oil and experimental group was given @entity11559 500 mg kg(-1) day(-1) by gavage. On GD 19.5 and postnatal day (PND) 45, the testes were removed. Morphological analysis of the testes was observed by transmission electron microscopy and @entity1710 and @entity1711 (H_E) staining. Testicular cell apoptosis was detected by terminal deoxynucleotidyl transferase @entity1977 nick end labeling (TUNEL). The expression of @entity1976 , @entity2310 , and @entity10740 was presented by immunohistochemistry (IHC) and western blot. Data of the two groups was compared using independent samples t-test and Mann-Whitney test by SPSS 20.0. RESULTS: H_E staining showed that spermatogenetic cells were significantly decreased in @entity11559 exposed pubertal @entity35 testis. The apoptosis index of testes in @entity11559 -treated group was significantly lower on GD 19.5 but higher on PND 45 than that of the controls ( p < 0.01). IHC and western blot revealed significantly increased expression of @entity1976 in GD 19.5 @entity35 testis and @entity2310 and @entity10740 in PND 45 @entity35 testis after @entity11559 exposure, compared with the control ( p < 0.05). CONCLUSION: In utero exposure of high-dose @entity11559 resulted in opposite effects on testicular cell apoptosis in late embryonic and pubertal @entity35 offspring. The overexpression of @entity1976 , @entity2310 , and @entity10740 might be related to the occurrence of abnormal apoptosis and finally produce @entity4810 .

In utero exposure of high-dose XXXX resulted in opposite effects on testicular cell apoptosis in late embryonic and pubertal male @entity35 offspring.

@entity10740 :: ('301300(Tax:10116)', 'Gene') :: ['p53'] @entity11559 :: ('CHEBI:35861', 'Chemical') :: ['DBP'] @entity1976 :: ('24224(Tax:10116)', 'Gene') :: ['Bcl-2'] @entity4810 :: ('MESH:D007248', 'Disease') :: ['male infertility'] @entity2310 :: ('24887(Tax:10116)', 'Gene') :: ['Bax'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity1977 :: ('CHEBI:17625', 'Chemical') :: ['dUTP'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hematoxylin'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity15523 :: ('MESH:D003993', 'Chemical') :: ['di- n-butyl phthalate']
@entity15523 :: (MESH:D003993,Chemical) :: ['di- n-butyl phthalate']
