Angiogenesis represents a compensatory response targeted to preserve the integrity of tissues subjected to @entity63 . The aim of the present study was to examine whether reparative angiogenesis is impaired in spontaneously @entity101 @entity35 (SHR), as a function of progression of @entity101 . In addition, the potential of gene therapy with @entity1 @entity40753 (HK) in revascularization was challenged in SHR and normotensive @entity35 -Kyoto @entity35 (WKY) that underwent excision of the left femoral artery. Expression of @entity7 and HK was upregulated in @entity63 hindlimb of WKY but not of SHR. Capillary density was increased in @entity63 adductor muscle of WKY (from 266+/-20 to 633+/-73 capillaries/mm(2) at 28 days, P<0.001), whereas it remained unchanged in SHR (from 276+/-20 to 354+/-48 capillaries/mm(2), P=NS), thus compromising perfusion recovery as indicated by @entity1003 flow ratio (0.61+/-0.08 versus 0.92+/-0.07 in WKY at 28 days, P<0.05). In separate experiments, saline or 5x10(9) pfu adenovirus containing the HK gene (Ad. @entity2834 -cHK) or the beta-galactosidase gene (Ad. @entity2834 -LacZ) was injected intramuscularly at 7 days after the induction of @entity63 . Ad. @entity2834 -cHK augmented capillary density and accelerated hemodynamic recovery in both strains, but these effects were more pronounced in SHR (P<0.01). Our results indicate that native angiogenic response to @entity63 is impaired in SHR, possibly as a result of defective modulation of endothelial cell mitogens. Supplementation with @entity7199 , one of the growth factors found to be deficient in SHR, restores physiological angiogenic response utilitarian for tissue healing. Our discoveries may have important implications in vascular medicine for therapeutic benefit.

Rescue of impaired angiogenesis in spontaneously @entity101 @entity35 by intramuscular @entity1 XXXX gene transfer.

@entity1 :: ('9606', 'Species') :: ['human'] @entity101 :: ('MESH:D006973', 'Disease') :: ['hypertension', 'hypertensive'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemic', 'ischemia'] @entity7 :: ('7422', 'Gene') :: ['vascular endothelial growth factor'] @entity40753 :: ('3816', 'Gene') :: ['tissue kallikrein'] @entity1003 :: ('MESH:D001778', 'Disease') :: ['reduced plantar blood'] @entity2834 :: ('12305', 'Species') :: ['CMV'] @entity35 :: ('10116', 'Species') :: ['Wistar', 'rats'] @entity7199 :: ('MESH:D007610', 'Chemical') :: ['kallikrein']
@entity40753 :: (3816,Gene) :: ['tissue kallikrein']
