UNASSIGNED: @entity519 is the main threat to morphological and functional integrity of isolated pancreatic islets. Lack of @entity26 seems to be of particular importance for functionality of encapsulated islets. The present study was initiated as an experimental model for the environment experienced by @entity1 islets in a confined space present during culture, shipment and in an implanted macrodevice. Quadruplicate aliquots of isolated @entity1 islets (n = 12) were cultured for 24 hours at 37  C in normoxia using 24-well plates equipped with 8  m-pore size filter inserts and filled with islet aliquots adjusted to obtain a seeding density of 75, 150, 300 or 600 IEQ/cm  . After culture viability, @entity413 -stimulated insulin release, DNA content as well as @entity589 and @entity588 gene expression were measured. Culture supernatants were collected to determine production of @entity7 and @entity2842 . Viability correlated inversely with IEQ seeding density (r = -0.71, p<0.001), while the correlation of @entity7 and @entity2842 secretion with seeding density was positive (r = 0.78, p<0.001; r = 0.54, p<0.001). Decreased viability corresponded with a significant increase of the @entity589 / @entity588 mRNA ratio at 300 and 600 IEQ/cm   and with a sigificantly reduced @entity413 -stimulated insulin secretion and insulin content compared to 75 or 150 IEQ/cm   (p<0.01). The present study demonstrates that the seeding density is inversely correlated with islet viability and in vitro function. This is associated with a significant increase of VEFG and @entity2842 release suggesting a @entity519 and proinflammatory islet microenvironment.

High Seeding Density Induces Local @entity519 and Triggers a Proinflammatory Response in Isolated XXXX Islets.

@entity1 :: ('9606', 'Species') :: ['human'] @entity589 :: ('581', 'Gene') :: ['Bax'] @entity588 :: ('596', 'Gene') :: ['Bcl-2'] @entity7 :: ('7422', 'Gene') :: ['VEGF'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity2842 :: ('6347', 'Gene') :: ['MCP-1'] @entity519 :: ('MESH:D000860', 'Disease') :: ['Hypoxia', 'hypoxic'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity1 :: (9606,Species) :: ['Human']
