OBJECTIVES: Bone marrow stromal cells (BMSC) transplantation proves successful in treating @entity8 and injury in many studies. However, their reparative capacity is limited by the poor homing ability in vivo, which is decided mainly by the local expression of chemoattractants. Our study explored the mechanical effects of @entity2835 ( @entity2835 ) on BMSCs homing ability in treated kidney tissues. METHODS: @entity35 were injected with red fluorescent protein (RFP)-labelled BMSCs and sonicated with microbubble-mediated ultrasound. Then, we tested kidney micro-environment changes induced and their influence on stem cell homing ability. RESULTS: The results showed that the mechanical effects of @entity2835 would increase local and transient levels of chemoattractants (i.e. cytokines, integrins and growth factors) in targeted kidney tissues. Transmission electron microscopy showed that vascular endothelial cell was discontinuous in the @entity2835 group post-treatment, becoming smooth 72  h later. Confocal laser scanning microscopy and RT-PCR showed up to eight times more stem cells in the peritubular regions of experimental kidneys on days 1 and 3 post-treatment compared with the contralateral kidney. CONCLUSIONS: These results confirmed that renal micro-environment changes caused by appropriate @entity2835 may promote BMSC homing ability toward treated kidney tissues without @entity8 and cell damage. KEY POINTS:     This experiment showed a feasible strategy in promoting stem cell homing ability.     The treatment uses diagnostic ultrasound during enhancement with IV microbubbles.     A suitable micro-environment was important for targeted stem cell homing and retention.     The method is effective for stem cell homing to @entity8 .     More work is required with larger animals before potential @entity1 trials.

Effects of diagnostic ultrasound-targeted microbubble destruction on the homing ability of bone marrow stromal cells to the XXXX .

@entity2835 :: ('MESH:D008105', 'Disease') :: ['ultrasound targeted microbubble destruction', 'UTMD'] @entity1 :: ('9606', 'Species') :: ['human'] @entity8 :: ('MESH:D007674', 'Disease') :: ['kidney diseases', 'renal toxicity', 'kidney disease'] @entity35 :: ('10116', 'Species') :: ['Rats']
@entity8 :: (MESH:D007674,Disease) :: ['kidney parenchyma']
