The aim of this study was to investigate the effect of @entity78 coating crystallinity and composition on the proliferation and differentiation of @entity35 bone marrow (RBM) cells. Grit-blasted @entity702 substrates were provided with thin sputter-coated @entity78 (Ca-P) films of different composition. The Ca-P-coated substrates were used as-sputtered or were heat-treated. XRD measurements showed that the as-sputtered coatings had an amorphous structure, whereas the heat-treated substrates showed an amorphous-crystalline structure. RBM cells were cultured on these substrates and on noncoated @entity702 substrates. After specific culture times, the expression of osteogenic markers by the cells was studied. On the amorphous-crystalline coatings as well as on @entity702 substrates, RBM cells proliferated, expressed alkaline phosphatase and showed mineralization. More mineralization was found on the amorphous-crystalline coatings than on the @entity702 substrates. Some precipitation was also found on substrates that were incubated in complete culture medium without cells. This precipitate disappeared after prolonged incubation. Alkaline phosphatase expression differed on the various amorphous-crystalline Ca-P-coated substrates, but no difference was found in the mineralization on these substrates. The amorphous Ca-P coatings showed extensive dissolution and some signs of precipitation after longer culture periods. Proliferation and differentiation of RBM cells was not seen on the amorphous coatings, regardless of Ca-P composition. We conclude that amorphous-crystalline Ca-P coatings stimulate differentiation of RBM cells, with only limited differences between coatings of various composition. In contrast, Ca-P coatings with an amorphous structure inhibit the growth and differentiation of RBM cells. This effect was found on all amorphous substrates, regardless of Ca-P composition.

Effect of XXXX coating composition and crystallinity on the response of osteogenic cells in vitro.

@entity35 :: ('10116', 'Species') :: ['rat'] @entity702 :: ('MESH:D014025', 'Chemical') :: ['titanium'] @entity78 :: ('MESH:C020243', 'Chemical') :: ['calcium phosphate']
@entity78 :: (MESH:C020243,Chemical) :: ['calcium phosphate']
