Many genes participate in the regulation of cell cycle progression from G1 to S phase. Functional loss of one or more of these genes has been reported to be associated with @entity105 and/or @entity5 progression and poor prognosis in many @entity5 . In a series of 126 @entity1 with @entity157 ( @entity157 ), we immunohistochemically evaluated @entity5 expression of the cell cycle-related gene protein products of Rb, @entity994 ( @entity994 ), and @entity876 . Positive immunostaining for Rb, @entity994 , and mutant @entity876 protein was detected in 58%, 33%, and 37% of the @entity5 , respectively. The proportion of HCCs exhibiting aberrant @entity876 protein expression increased significantly with advancing stage of disease (p < 0.001), poorer histological classification of differentiation (p < 0.01), and increasing @entity5 size (p < 0.01). A decrease in the proportion of HCCs expressing @entity994 protein was also associated with advancing clinical stage of disease (p < 0.01), and larger @entity5 size (p < 0.05). The only clinicopathological feature found to be associated with Rb status, was intrahepatic @entity3 , which occurred with a higher frequency in HCCs exhibiting positive immunoreactivity for Rb protein expression (p < 0.05). Multivariate survival analysis revealed that, amongst the protein products of the different genes evaluated, only positive immunostaining for aberrant @entity876 protein expression served as an independent prognostic indicator, being significantly associated with worse survival in @entity1 with @entity157 (p = 0.023). Analysis for relationships between gene products showed an inverse correlation between expression of aberrant @entity876 protein and @entity994 protein (p < 0.01), and also an inverse correlation between @entity994 protein and Rb protein expression (p < 0.05) in these cases of @entity157 . These findings demonstrate that positive immunostaining for mutant @entity876 protein expression is a significant indicator of @entity5 progression and poor prognosis, confirm that @entity994 protein expression is induced in a @entity876 -dependent manner, and suggest that Rb protein expression may be regulated to some extent by @entity994 in @entity157 .

Protein expression of @entity876 , p21WAF1, and Rb as prognostic indicators in XXXX with surgically treated @entity157 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancers', 'tumors'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity157 :: ('MESH:D006528', 'Disease') :: ['HCC', 'hepatocellular carcinoma'] @entity105 :: ('MESH:D063646', 'Disease') :: ['carcinogenesis'] @entity994 :: ('1026', 'Gene') :: ['p21', 'WAF1']
@entity1 :: (9606,Species) :: ['patients']
