UNASSIGNED: @entity5721 ( @entity5721 ) is a primary concern in liver transplantation especially when @entity1560 is present. @entity3791 (AZ), a specific carbonic anhydrases (CAs) inhibitor, has been suggested to protect against @entity519 . Here, we hypothesized that AZ administration could be @entity1560 against cold @entity5721 . Obese Zucker @entity35 livers were preserved in Institut Georges Lopez (IGL-1) storage solution for 24 hours at 4  C and "ex vivo" perfused for 2 hours at 37  C. Alternatively, @entity35 were also treated with intravenous injection of AZ (30 mg/kg) before liver recovery. @entity99 , hepatic function and vascular resistance were determined. @entity1250 protein levels and CA hydratase activity were assessed, as well as other parameters involved in @entity5721 (endothelial @entity842 synthase (eNOS), mitogen activated protein kinases (MAPKs) family, @entity519 inductible factor 1 alpha ( @entity16705 ) and @entity5301 ( @entity5301 )). We demonstrated that AZ administration protects efficiently steatotic liver against cold @entity5721 . AZ protection was associated with better function, decreased vascular resistance and activation of eNOS. This was consistent with an effective MAPKs inactivation. Finally, no effect on @entity16705 / @entity5301 pathway was observed. The present study demonstrated that AZ administration is a suitable pharmacological strategy for preserving @entity1560 against cold @entity5721 .

XXXX Protects Steatotic Liver Grafts against Cold Ischemia Reperfusion Injury.

@entity5721 :: ('MESH:D015427', 'Disease') :: ['Ischemia reperfusion injury', 'IRI'] @entity1560 :: ('MESH:D005234', 'Disease') :: ['efficient to protect fatty livers', 'fatty liver grafts', 'steatosis'] @entity3791 :: ('MESH:D000086', 'Chemical') :: ['Acetazolamide'] @entity5301 :: ('24335(Tax:10116)', 'Gene') :: ['erythropoietin', 'Epo'] @entity519 :: ('MESH:D000860', 'Disease') :: ['hypoxic', 'hypoxia'] @entity16705 :: ('29560(Tax:10116)', 'Gene') :: ['HIF-1a'] @entity1250 :: ('54231(Tax:10116)', 'Gene') :: ['CA II'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity99 :: ('MESH:D056486', 'Disease') :: ['Liver injury']
@entity3791 :: (MESH:D000086,Chemical) :: ['Acetazolamide']
