@entity7 and its @entity4149 kinase receptors have been identified as key mediators of the regulation of pathologic blood vessel growth and maintenance in the promotion of angiogenesis and @entity5 growth. Therefore, an alternative approach to destroying @entity5 endothelium would be to make this tissue particularly sensitive to @entity7 -mediated drug delivery. To verify this hypothesis, we generated a protein containing @entity1655 fused to melittin. Melittin is a small linear peptide composed of 26 @entity3689 residues that can exert toxic or inhibitory effects on many types of @entity5 cells. This protein is a cytolytic peptide that attacks lipid membranes, leading to significant @entity137 . In the present study, the @entity10056 expression system was used to express the fusion protein. Under optimal conditions, stable @entity1655 -melittin production was achieved using a series of purification steps. The activity of @entity1655 -melittin fusion protein was compared with melittin for its ability to suppress the growth of @entity5 cell line in  vitro. The fusion toxin selectively inhibited growth of @entity1 @entity157 HepG-2 cell line with high expression of @entity319 . We found that sensitivity of @entity319 transfected 293 cells to @entity1655 -melittin enhanced as the cellular @entity319 density increased. In an in  vivo initial experiment, the fusion protein inhibited @entity5 growth in xenografts assays. Furthermore, successful expression and characterization of the fusion protein demonstrated its efficacy for use as a novel treatment strategy for @entity5 .

Preparation and functional characterization of @entity1 XXXX -melittin fusion protein with analysis of the antitumor activity in vitro and in vivo.

@entity1 :: ('9606', 'Species') :: ['human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity7 :: ('7422', 'Gene') :: ['VEGF', 'Vascular endothelial growth factor'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity10056 :: ('4922', 'Species') :: ['Pichia pastoris'] @entity319 :: ('3791', 'Gene') :: ['VEGFR-2'] @entity1655 :: ('MESH:C467484', 'Chemical') :: ['VEGF165'] @entity3689 :: ('D000596', 'Chemical') :: ['amino acid'] @entity157 :: ('MESH:D006528', 'Disease') :: ['hepatocellular carcinoma'] @entity4149 :: ('D014443', 'Chemical') :: ['tyrosine']
@entity7 :: (7422,Gene) :: ['vascular endothelial growth factor']
