MAIN CONCLUSION: @entity972 -permeable slow activating vacuolar channels (SV) and @entity2865 -permeable channels in the vacuole of the @entity42721 @entity42721 were characterized in respect to @entity460 dependence, selectivity, and pharmacology. The patch-clamp method was used in the study of ion channel activity in the vacuoles from the @entity42721 @entity42721 . The whole-vacuole recordings allowed simultaneous observation of two types of currents-predominant slow activated currents recorded at positive voltages and fast activated currents recorded at negative voltages. Single-channel recordings carried out in the gradient of @entity10406 indicated that slow activated currents were carried by @entity972 -permeable slowly activating vacuolar channels (SV) and fast activated currents-by @entity2865 -permeable channels. Both types of the channels were dependent in an opposite way on @entity460 , since elimination of this ion from the cytoplasmic side caused inhibition of SV channels, but the open probability of @entity2865 -permeable channels even increased. The dependence of the activity of both channels on different types of ion channel inhibitors was studied. SV channels exhibited different sensitivity to @entity972 channel inhibitors. These channels were insensitive to 3  mM @entity8594 , but were blocked by 3  mM @entity5128 ( @entity1041 ). Moreover, the activity of the channels was modified in a different way by @entity460 channel inhibitors. 200    M @entity15311 was an effective blocker, but 50    M @entity3739 red evoked bursts of the channel activity resulting in an increase in the open probability. Different effectiveness of anion channel inhibitors was observed in @entity2865 -permeable channels. After the application of 100    M @entity8862 , a decrease in the open probability was recorded but the channels were still active. 50    M DIDS was more effective, as it completely blocked the channels.

Vacuolar ion channels in the @entity42721 XXXX : influence of ion channel inhibitors.

@entity8862 :: ('CHEBI:37256', 'Chemical') :: ['Zn(2+)'] @entity972 :: ('MESH:D011188', 'Chemical') :: ['Potassium', 'potassium'] @entity1041 :: ('MESH:D013662', 'Chemical') :: ['TEA'] @entity42721 :: ('3197', 'Species') :: ['liverwort', 'Marchantia polymorpha'] @entity10406 :: ('C522374', 'Chemical') :: ['KCl'] @entity5128 :: ('MESH:D019789', 'Chemical') :: ['tetraethyl ammonium'] @entity15311 :: ('MESH:C026226', 'Chemical') :: ['Gd(3+)'] @entity3739 :: ('MESH:D012428', 'Chemical') :: ['ruthenium'] @entity8594 :: ('MESH:C080430', 'Chemical') :: ['Ba(2+)'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity2865 :: ('CHEBI:17996', 'Chemical') :: ['chloride']
@entity42721 :: (3197,Species) :: ['Marchantia polymorpha']
