PURPOSE: We investigated whether @entity9381 injected in the bladder would affect preganglionic parasympathetic nerve endings in intramural ganglia. MATERIALS AND METHODS: @entity840 bladders were injected with 5 U of botulinum toxin. At 24 hours bladders were collected and processed for immunohistochemistry using @entity669 hydroxylase, and intact and cleaved @entity28124 . To identify the different populations of affected fibers coursing the ganglia we performed double immunoreactions for cleaved @entity28124 and VAChT, TH or CGRP. RESULTS: VAChT immunoreactive fibers were identified in axons and varicosities of presynaptic to postganglionic parasympathetic neurons. Those fibers were also immunoreactive to SV2 and @entity28124 . The rare CGRP and TH immunoreactive fibers coursing in the ganglia did not express SV2 or @entity28124 . After @entity9381 injection the cleaved form of @entity28124 was abundantly expressed in parasympathetic fibers. CONCLUSIONS: Botulinum toxin injection in the bladder wall affects preganglionic parasympathetic nerve terminals. This could contribute to the strong effect of botulinum toxin on bladder smooth muscle activity.

Effect of XXXX on intramural parasympathetic ganglia: an experimental study in the @entity840 bladder.

@entity28124 :: ('100724783', 'Gene') :: ['SNAP-25'] @entity840 :: ('10141', 'Species') :: ['Guinea pig'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine'] @entity9381 :: ('MESH:C542870', 'Chemical') :: ['onabotulinumtoxinA']
@entity9381 :: (MESH:C542870,Chemical) :: ['onabotulinumtoxinA']
