Three experimental approaches were used to examine the maturation of @entity1024 ( @entity143 ) receptors in the neostriatum and compare their developmental profile to that of the non- @entity143 receptors [ @entity140 ( @entity140 ) and @entity4176 (KA)]. The first, and least conventional approach utilized infrared videomicroscopy to measure @entity143 -induced swelling in single cells in a brain slice. The results demonstrated that @entity143 receptors display an incremental pattern of postnatal development with no responses at postnatal day (PND) 3, weak responses at PND 7, the largest responses by PND 14 and slight decreases at PNDs 21 and 28. At PNDs 3 and 7, KA-induced cell swelling was proportionately greater than @entity143 -induced cell swelling suggesting earlier maturation of this non- @entity143 receptor subtype. The second approach used whole-cell patch clamp analysis to examine @entity143 currents and compare their maturation to @entity140 /KA-induced currents. Though the data are still preliminary, a very similar developmental pattern emerged. @entity143 -induced currents were small and developed slowly after PND 7. In contrast, @entity140 /KA-induced currents were larger and appeared to develop earlier. Finally, @entity3390 ( @entity3390 ) binding was measured in homogenates of neostriatal tissue. The ontogeny of binding resembled a step function with increases between PNDs 3 and 7 and PNDs 14 and 21. Binding peaked at PND 28 and then declined slightly in the adult (PND 60). The affinity of @entity3390 for the receptor did not change during postnatal development. These findings demonstrate the pattern of functional development of @entity2227 receptors in the neostriatum. The @entity143 receptor subtype displays minimal functional development until PND 14. In contrast, neostriatal @entity140 /KA receptor function appears to precede @entity143 receptor function.

Postnatal development of XXXX receptor-mediated responses in the neostriatum.

@entity1024 :: ('CHEBI:31882', 'Chemical') :: ['N-methyl-D-aspartate'] @entity2227 :: ('CHEBI:29987', 'Chemical') :: ['glutamate'] @entity4176 :: ('MESH:D007608', 'Chemical') :: ['kainate'] @entity140 :: ('MESH:D018350', 'Chemical') :: ['alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid', 'AMPA'] @entity143 :: ('CHEBI:6121', 'Chemical') :: ['NMDA'] @entity3390 :: ('MESH:D016291', 'Chemical') :: ['MK-801', 'dizocilpine']
@entity2227 :: (CHEBI:29987,Chemical) :: ['glutamate']
