@entity1358 ( @entity1358 ) frequently metastasizes to the bone marrow. These metastases are characterized by extensive @entity690 . The mechanism, however, by which @entity1358 cells metastasize to bone marrow remains poorly understood. To unravel the role of bone marrow cells in this context, we performed cell adhesion and migration assays using @entity1 @entity1358 cell lines to analyze the influence of resident bone marrow cells on @entity703 cells. The strongest adhesion of @entity1358 cells was observed to osteoblasts. Moreover, conditioned medium of osteoblasts (OB-CM) significantly increased @entity1358 cell migration. By gene expression analysis @entity34614 was identified as a transcript whose expression could be elevated more than twofold in @entity1358 cells when exposed to OB-CM. Suppression of @entity34614 expression in @entity1358 cells by siRNA reduced their ability to migrate in the presence of OB-CM. Furthermore, the @entity1358 cells secreted high amounts of the chemokine @entity2842 when @entity5 cells migrated under the influence of osteoblast-secreted factors. @entity2842 neutralization strongly reduced the migratory ability of the @entity1358 cells. Silencing the expression of @entity34614 in @entity1358 cells resulted in a twofold reduction of @entity2842 protein expression indicating a @entity34614 -dependent expression of the chemokine. Taken together, our data show that osteoblasts are the major cell type of the bone marrow that affect @entity1358 cells by secreting factors that increase the expression of @entity34614 and @entity2842 in the @entity5 cells leading to enhanced cell migration. These data suggest an osteoblast-induced autocrine mechanism for a facilitated homing of @entity1358 cells to the bone marrow.

Osteoblast-secreted factors enhance the expression of XXXX and @entity2842 -dependent migration of @entity703 cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity34614 :: ('53827', 'Gene') :: ['dysadherin'] @entity2842 :: ('6347', 'Gene') :: ['CCL2'] @entity1358 :: ('MESH:D002292', 'Disease') :: ['Renal cell carcinoma', 'RCC'] @entity690 :: ('MESH:D030981', 'Disease') :: ['osteolytic lesions'] @entity703 :: ('MESH:D007680', 'Disease') :: ['renal tumor']
@entity34614 :: (53827,Gene) :: ['dysadherin']
