The metabolism of high grade @entity816 was studied in 15 @entity1 using intra-tumoural microdialysis. Two catheters were implanted during a stereotactic biopsy procedure: one in the @entity5 and one in the peri-tumoural tissue. The @entity1 were fully mobilized the same day as the operation. Microdialysis samples were collected the next day and subsequently analysed for @entity413 , @entity880 , @entity2388 , @entity2227 and @entity653 . The main objective was to establish base-line levels of the studied substances. In addition, an in vitro experiment was performed in order to estimate recovery for the flow rates and catheters used. @entity413 showed a tendency to be lower in @entity5 than in peri-tumoural tissue, indicating a high energy demand of the @entity5 . @entity880 was significantly higher in @entity5 tissue. This supports previous reports that high grade astrocytomas use glycolysis rather than respiration to meet their energy demand. The @entity5 were also classified into necrotic and non-necrotic, according to the radiological finding. The @entity5 showed significantly higher levels of @entity2227 . They also presented a tendency to higher levels of @entity653 than the @entity5 . These findings might be explained by the release of intracellular @entity2227 and of cell-membrane @entity653 by cell destruction. We believe that microdialysis in awake and mobilized @entity1 will prove to be a valuable tool in investigating metabolic events in malignant @entity1313 especially during therapy.

Baseline levels of XXXX metabolites, @entity2227 and @entity653 in malignant glioma assessed by stereotactic microdialysis.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity2227 :: ('CHEBI:29987', 'Chemical') :: ['glutamate'] @entity1313 :: ('MESH:D001932', 'Disease') :: ['brain tumours'] @entity5 :: ('MESH:D009369', 'Disease') :: ['necrotic tumours', 'tumours', 'non-necrotic tumours', 'tumour'] @entity653 :: ('MESH:D005990', 'Chemical') :: ['glycerol'] @entity816 :: ('MESH:D001254', 'Disease') :: ['astrocytoma'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['Lactate', 'lactate'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose', 'Glucose'] @entity2388 :: ('MESH:D019289', 'Chemical') :: ['pyruvate']
@entity413 :: (MESH:D005947,Chemical) :: ['glucose']
