Mitotic and cytokinetic processes harness cell machinery to drive chromosomal segregation and the physical separation of dividing cells. Here, we investigate the functional requirements for exocyst complex function during cell division in vivo, and demonstrate a common mechanism that directs anaphase cell elongation and cleavage furrow progression during cell division. We show that onion rings (onr) and funnel cakes (fun) encode the @entity238 homologs of the @entity69844 and @entity69845 exocyst subunits, respectively. In onr and fun mutant cells, contractile ring proteins are recruited to the equatorial region of dividing spermatocytes. However, cytokinesis is disrupted early in @entity8145 , leading to cytokinesis failure. We use high temporal and spatial resolution confocal imaging with automated computational analysis to quantitatively compare wild-type versus onr and fun mutant cells. These results demonstrate that anaphase cell elongation is grossly disrupted in cells that are compromised in exocyst complex function. Additionally, we observe that the increase in cell surface area in wild type peaks a few minutes into cytokinesis, and that onr and fun mutant cells have a greatly reduced rate of surface area growth specifically during cell division. Analysis by transmission electron microscopy reveals a massive build-up of cytoplasmic astral membrane and loss of normal Golgi architecture in onr and fun spermatocytes, suggesting that exocyst complex is required for proper vesicular trafficking through these compartments. Moreover, recruitment of the small GTPase @entity44339 and the @entity69846 Giotto to the cleavage site depends on wild-type function of the exocyst subunits @entity69844 and @entity69845 . Finally, we show that the exocyst subunit @entity69847 coimmunoprecipitates with @entity44339 . Our results are consistent with the exocyst complex mediating an essential, coordinated increase in cell surface area that potentiates anaphase cell elongation and cleavage @entity8145 .

Exocyst-Dependent Membrane Addition Is Required for Anaphase Cell Elongation and Cytokinesis in XXXX .

@entity69844 :: ('43163(Tax:7227)', 'Gene') :: ['Exo84'] @entity69845 :: ('40712(Tax:7227)', 'Gene') :: ['Sec8'] @entity69847 :: ('33563(Tax:7227)', 'Gene') :: ['Sec5'] @entity8145 :: ('MESH:D014063', 'Disease') :: ['furrow ingression'] @entity69846 :: ('42306(Tax:7227)', 'Gene') :: ['PITP'] @entity44339 :: ('42501(Tax:7227)', 'Gene') :: ['Rab11'] @entity238 :: ('7227', 'Species') :: ['Drosophila']
@entity238 :: (7227,Species) :: ['Drosophila']
