PURPOSE: The purpose of this study was to explore gene expression changes in fatigued @entity1 with nonmetastatic @entity263 receiving localized external beam radiation therapy (EBRT). METHODS: Fatigue was measured in 40 @entity1 with @entity263 (20 receiving EBRT and 20 controls on active surveillance) using the Functional Assessment of Cancer Therapy-Fatigue (FACT-F). EBRT subjects were followed from baseline to midpoint and end point of EBRT, while controls were seen at one time point. EBRT subjects were categorized into high- and low- @entity1295 groups based on change in FACT-F scores from baseline to EBRT completion. Full genome microarray was performed from peripheral leukocyte RNA to determine gene expression changes related to @entity1295 phenotypes. Real-time polymerase chain reaction and enzyme-linked immunosorbent assay confirmed the most differentially expressed gene in the microarray experiment. RESULTS: At baseline, mean FACT-F scores were not different between EBRT subjects (44.3    7.16) and controls (46.7    4.32, p = .24). Fatigue scores of EBRT subjects decreased at treatment midpoint (38.6    9.17, p = .01) and completion (37.6    9.9, p = .06), indicating worsening @entity1295 . Differential expression of 42 genes was observed between @entity1295 groups when EBRT time points were controlled. Membrane-spanning four domains, subfamily A, member ( @entity12950 ) was the most differentially expressed gene and was associated with @entity1295 at treatment end point (r = -.46, p = .04). CONCLUSION: Fatigue intensification was associated with @entity12950 downregulation, suggesting that @entity1295 during EBRT may be related to impairment in B-cell immune response. The 42 differentially expressed @entity1295 -related genes are associated with @entity2041 biosynthesis, y-glutamyl cycle, and antigen presentation pathways.

Genomic Profile of Fatigued XXXX Receiving Localized Radiation Therapy.

@entity1 :: ('9606', 'Species') :: ['men'] @entity263 :: ('MESH:D011471', 'Disease') :: ['prostate cancer'] @entity2041 :: ('MESH:D005978', 'Chemical') :: ['glutathione'] @entity1295 :: ('MESH:D005221', 'Disease') :: ['fatigue'] @entity12950 :: ('931', 'Gene') :: ['MS4A1']
@entity1 :: (9606,Species) :: ['Men']
