Spongospora subterranea is responsible for significant @entity415 root and @entity66 globally. Study of this obligate (non-culturable) pathogen that infects below-ground plant parts is technically difficult. The capacity to measure the dynamics and patterns of root infections can greatly assist in determining the efficacy of control treatments on disease progression. This study used qPCR and histological analysis in time-course experiments to measure temporal patterns of pathogen multiplication and disease development in @entity415 (and @entity523 ) roots and tubers. Effects of delayed @entity281 and fungicidal seed tuber and soil treatments were assessed. This study found roots at all plant developmental ages were susceptible to @entity281 but that delaying @entity281 significantly reduced pathogen content and resultant disease at final harvest. The pathogen was first detected in roots 15-20 days after inoculation (DAI) and the presence of zoosporangia noted 15-45 DAI. Following initial @entity281 pathogen content in roots increased at a similar rate regardless of plant age at inoculation. All fungicide treatments (except soil-applied mancozeb which had a variable response) suppressed pathogen multiplication and root and @entity66 . In contrast to delayed inoculation, the fungicide treatments slowed disease progress (rate) rather than delaying onset of @entity281 . Trials under suboptimal temperatures for disease expression provided valuable data on root @entity281 rate, demonstrating the robustness of monitoring root @entity281 . These results provide an early measure of the efficacy of control treatments and indicate two possible patterns of disease suppression by either delayed @entity281 which then proceeds at a similar rate or diminished epidemic rate.

Monitoring Spongospora subterranea Development in XXXX Roots Reveals Distinct Infection Patterns and Enables Efficient Assessment of Disease Control Methods.

@entity415 :: ('4113', 'Species') :: ['potato'] @entity523 :: ('4081', 'Species') :: ['tomato'] @entity66 :: ('MESH:D004194', 'Disease') :: ['tuber disease'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection', 'initiation of infection']
@entity415 :: (4113,Species) :: ['Potato']
