RATIONALE AND OBJECTIVES: The aim of the present study was to determine the possible mechanisms underlying the endothelial cell damage induced by iodinated @entity8 ( @entity8 ). METHODS: The cultured @entity1 skin microvascular endothelial cells (HMVECs) were exposed to various contrast media, and the cell viability was measured by mitochondrial enzyme activity. Nuclear damage was assessed by @entity5956 staining and a fluorescent single-cell gel electrophoresis. The effects of contrast materials on the cellular @entity855 content and intracellular free @entity141 concentration were subsequently examined. RESULTS: Although the iodinated @entity8 tested all caused the cell injury in HMVECs, ionic @entity8 including @entity4946 and @entity7567 were more potent in producing the cell damage than nonionic @entity8 . It is unlikely that the contrast material-induced cell damage is associated with hyperosmolality, since hyperosmolar solution of @entity2656 or @entity1219 had no marked influence on the endothelial cell viability. Nuclear damage was noted in cells exposed to @entity4946 . @entity4946 lowered cellular @entity855 content while elevating the intracellular free @entity141 concentration. It was notable that the @entity8 -induced endothelial cell damage was reversed by the chelation of intracellular @entity141 with @entity18650 but not by the removal of extracellular @entity141 . CONCLUSIONS: Both ionic and nonionic contrast materials caused nuclear damage of endothelial cells. The decrease in tissue @entity855 content and elevation of intracellular @entity141 are likely to contribute to the contrast materials-induced endothelial cell damage.

Calcium-dependent injury of XXXX microvascular endothelial cells induced by a variety of iodinated radiographic contrast media.

@entity1 :: ('9606', 'Species') :: ['human'] @entity2656 :: ('MESH:D008353', 'Chemical') :: ['mannitol'] @entity8 :: ('MESH:D007674', 'Disease') :: ['RCM', 'radiographic contrast materials'] @entity5956 :: ('MESH:C017807', 'Chemical') :: ['Hoechst 33342'] @entity1219 :: ('CHEBI:26710', 'Chemical') :: ['NaCl'] @entity4946 :: ('MESH:D003973', 'Chemical') :: ['Amidotrizoate', 'amidotrizoate'] @entity7567 :: ('MESH:D007485', 'Chemical') :: ['ioxaglate'] @entity18650 :: ('MESH:C025603', 'Chemical') :: ["1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetra-acetic acid"] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity1 :: (9606,Species) :: ['human']
