UNASSIGNED: The expansion of myogenic progenitors (MPs) in the adult muscle stem cell niche is critical for the regeneration of skeletal muscle. Activation of quiescent MPs depends on the dismantling of the basement membrane and increased access to growth factors such as @entity540 ( @entity540 ). Here, we demonstrate using microRNA (miRNA) profiling in @entity19 and @entity1 myoblasts that the capacity of @entity540 to stimulate myoblast proliferation is mediated by @entity695 . @entity540 induces @entity695 expression and inhibition of @entity695 using pharmacological or genetic deletion decreases myoblast proliferation. Next generation RNA sequencing from @entity695 knockout myoblasts ( @entity4739 (CE/+) ; @entity695 (flox/flox) ) identified members of the basement membrane as the most abundant @entity695 targets. Using gain- and loss-of-function experiments, we confirm that @entity695 coordinately regulates @entity6099 , @entity41993 , @entity21510 , @entity5938 , @entity9294 and @entity9618 in myoblasts in vitro and in MPs in vivo. Induction of @entity540 and @entity695 and downregulation of its target genes precedes muscle regeneration during cardiotoxin (CTX)-induced @entity121 . Importantly, MP-specific @entity440 -induced deletion of @entity695 in adult skeletal muscle decreased the proliferation and formation of newly formed myofibers during both CTX-induced @entity121 and after a single bout of eccentric exercise. Our results identify a novel miRNA-based checkpoint of the basement membrane in the adult muscle stem cell niche. Strategies targeting @entity695 might provide useful clinical approaches to maintain muscle mass in disease states such as ageing that involve aberrant @entity540 signaling. Stem Cells 2016.

MicroRNA-29a in Adult Muscle Stem Cells Controls Skeletal Muscle Regeneration During Injury and Exercise Downstream of XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity21510 :: ('22795', 'Gene') :: ['Nid2'] @entity9618 :: ('6678', 'Gene') :: ['Sparc'] @entity41993 :: ('3915', 'Gene') :: ['Lamc1'] @entity440 :: ('MESH:D013629', 'Chemical') :: ['tamoxifen'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity695 :: ('407021', 'Gene') :: ['miR-29a'] @entity6099 :: ('2200', 'Gene') :: ['Fbn1'] @entity9294 :: ('3339', 'Gene') :: ['Hspg2'] @entity540 :: ('2247', 'Gene') :: ['fibroblast growth factor-2', 'FGF2'] @entity4739 :: ('5081', 'Gene') :: ['Pax7'] @entity5938 :: ('1282', 'Gene') :: ['Col4a1'] @entity121 :: ('MESH:D009135', 'Disease') :: ['muscle injury']
@entity540 :: (2247,Gene) :: ['Fibroblast Growth Factor-2']
