OBJECTIVE: Micro RNAs (miRNAs) are a class of non-coding regulatory RNAs. We performed a transcriptome-wide analysis of subcutaneous adipose tissue and in vitro studies to identify miRNAs and co-regulated target transcripts associated with insulin sensitivity (SI) and @entity28 in @entity1 . METHODS: We selected 20 insulin-resistant (IR, SI=2.0  0.7) and 20 insulin-sensitive (IS, SI=7.2  2.3) subjects from a cohort of 117 metabolically characterized non-diabetic Caucasians for comparison. RESULTS: After global profiling, 3 miRNAs had marginally different expressions between IR and IS subjects. A total of 14 miRNAs were significantly correlated with %fat mass, body mass index (BMI), or SI. The qRT-PCR validated the correlation of @entity11216 -148a-3p with BMI (r=-0.70, P=2.73X10-6). MiRNA target filtering analysis identified @entity16610 ( @entity16610 ) as one of the target genes of @entity11216 -148a-3p. @entity16610 expression in adipose tissue was positively correlated with BMI (r=0.47, p=8.42X10-7) and was inversely correlated with @entity11216 -148a-3p (r=-0.34). Differentiation of @entity21117 preadipocytes showed up-regulation of @entity11216 -148a-3p and down-regulation of @entity16610 in differentiated adipocytes. After transfecting @entity11216 -148a-3p mimics into HeLa-S3 cells, @entity16610 was down-regulated, while transfection of adipose stem cells with @entity11216 -148a-3p inhibitor up-regulated @entity16610 . CONCLUSIONS: Our results indicate that @entity11216 -148a-3p-mediated regulation of @entity16610 expression may play a mechanistic role in @entity28 .

Obesity associated modulation of miRNA and co-regulated target transcripts in XXXX adipose tissue of non-diabetic subjects.

@entity1 :: ('9606', 'Species') :: ['human'] @entity28 :: ('MESH:D009765', 'Disease') :: ['obesity'] @entity11216 :: ('220972', 'Gene') :: ['miR'] @entity21117 :: ('MESH:C537340', 'Disease') :: ['SGBS'] @entity16610 :: ('1786', 'Gene') :: ['DNA methyltransferase 1', 'DNMT1']
@entity1 :: (9606,Species) :: ['human']
