UNASSIGNED: We evaluated the bone regeneration and healing effect of Medicarpin (med) in cortical @entity79 model that heals by intramembranous ossification. For the study, female @entity35 were ovariectomized and rendered @entity6092 . A drill hole injury was generated in mid femoral bones of all the animals. Med treatment was commenced the day after and continued for 15 days. @entity4727 was taken as a reference standard. Fifteen days post-treatment, animals were sacrificed. Bones were collected for histomorphometry studies at the injury site by micro-computed tomography ( CT) and confocal microscopy. RNA and protein was harvested from newly generated bone. For immunohistochemistry, 5 m sections of decalcified femur bone adjoining the drill hole site were cut. By  CT analysis and @entity5384 labeling of newly generated bone it was found that med promotes @entity79 and new bone formation at the injury site and was comparable to @entity4727 in many aspects. Med treatment led to increase in the @entity6037 and @entity9356 signals indicating expansion of osteoprogenitors at the injury site as evaluated by qPCR and immunohistochemical localization. It was observed that med promoted bone regeneration by activating canonical @entity29972 and notch signaling pathway. This was evident by increased transcript and protein levels of @entity29972 and notch signaling components in the defect region. Finally, we confirmed that med treatment leads to elevated @entity79 in pre-osteoblasts by co localization of beta catenin with osteoblast marker alkaline phosphatase. In conclusion, med treatment promotes new bone regeneration and healing at the injury site by activating @entity29972 /canonical and notch signaling pathways. This study also forms a strong case for evaluation of med in delayed union and non-union @entity174 cases.

Medicarpin, a Natural Pterocarpan, Heals Cortical Bone Defect by Activation of Notch and XXXX Canonical Signaling Pathways.

@entity6037 :: ('367218(Tax:10116)', 'Gene') :: ['Runx-2'] @entity174 :: ('MESH:D050723', 'Disease') :: ['fracture'] @entity79 :: ('MESH:D001847', 'Disease') :: ['bone defect', 'bone healing'] @entity6092 :: ('MESH:C567172', 'Disease') :: ['osteopenic'] @entity9356 :: ('81613(Tax:10116)', 'Gene') :: ['osteocalcin'] @entity35 :: ('10116', 'Species') :: ['Sprague-Dawley rats'] @entity4727 :: ('24694(Tax:10116)', 'Gene') :: ['PTH'] @entity5384 :: ('MESH:C007740', 'Chemical') :: ['calcein'] @entity29972 :: ('114487(Tax:10116)', 'Gene') :: ['Wnt']
@entity29972 :: (114487(Tax:10116),Gene) :: ['Wnt']
