The persistence of @entity1800 that it causes in the @entity1 population can be attributed to the maintenance of a @entity281 within neurons in sensory ganglia. Little is known about the effects of @entity281 on the host neuron. We have addressed the question of whether latent @entity2025 affects neuronal gene expression by using microarray transcript profiling of host gene expression in ganglia from latently infected versus mock-infected @entity19 trigeminal ganglia. @entity69967 -labeled cDNA probes from pooled ganglia harvested at 30 days postinfection or post-mock infection were hybridized to nylon arrays printed with 2,556 @entity19 genes. Signal intensities were acquired by phosphorimager. Mean intensities (n = 4 replicates in each of three independent experiments) of signals from mock-infected versus latently infected ganglia were compared by using a variant of Student's t test. We identified significant changes in the expression of @entity19 neuronal genes, including several with roles in gene expression, such as the @entity69968 gene, and neurotransmission, such as genes encoding @entity972 voltage-gated channels and a muscarinic @entity934 receptor. We confirmed the neuronal localization of some of these transcripts by using in situ hybridization. To validate the microarray results, we performed real-time reverse transcriptase PCR analyses for a selection of the genes. These studies demonstrate that latent @entity2025 can alter neuronal gene expression and might provide a new mechanism for how persistent @entity1800 can cause @entity102 .

XXXX of sensory neurons alters neuronal gene expression.

@entity1 :: ('9606', 'Species') :: ['human'] @entity69967 :: ('CHEBI:37973', 'Chemical') :: ['(33)P'] @entity972 :: ('MESH:D011188', 'Chemical') :: ['potassium'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity102 :: ('MESH:D002908', 'Disease') :: ['chronic disease'] @entity281 :: ('MESH:D007239', 'Disease') :: ['latent infection'] @entity1800 :: ('MESH:D014777', 'Disease') :: ['herpes simplex virus (HSV) and the diseases', 'viral infection'] @entity2025 :: ('MESH:D020031', 'Disease') :: ['HSV infection'] @entity69968 :: ('12748(Tax:10090)', 'Gene') :: ['Clk2'] @entity934 :: ('MESH:D000109', 'Chemical') :: ['acetylcholine']
@entity2025 :: (MESH:D020031,Disease) :: ['Latent herpes simplex virus infection']
