BACKGROUND: Ongoing complement activation in @entity1 with a ventricular assist device may contribute to observed @entity4461 and cellular aggregation by mediating leukocyte and platelet activation, formation of leukocyte-platelet conjugates, and the tissue factor pathway of coagulation. METHODS: Blood from 30 @entity1 was collected before ventricular assist device implantation and during the implantation period. Plasma levels of @entity556 -antithrombin III complexes, @entity4417 , and SC5b-9 were measured by commercial enzyme-linked immunosorbent assay. Flow cytometry was used to measure circulating monocyte tissue factor expression and circulating monocyteplatelet and granulocyte-platelet conjugates. RESULTS: @entity556 -antithrombin III complex level and monocyte tissue factor expression peaked in the early postoperative period, with maxima occurring on postoperative days 5 and 3, respectively. Levels of @entity4417 and SC5b-9 remained dramatically elevated over normal values for the duration of the study (6 and 5 times upper normal, respectively). Levels of monocyte-platelet conjugates were normal before implantation, decreased during the first 4 postoperative days, and then increased and remained elevated. Levels of granulocyte-platelet conjugates were elevated over the normal range before implantation and remained elevated from postoperative days 3 to 21. A positive correlation was found between levels of SC5b-9 and granulocyte-platelet conjugates (Spearman R=0.66; p < 0.001), and between levels of @entity4417 and @entity556 -antithrombin III complex (Spearman R=0.13; p=0.021). CONCLUSIONS: The data suggest a model in which complement mediates formation of @entity1423 and may indirectly contribute to @entity556 generation through monocyte tissue factor expression.

Monocyte tissue factor expression and ongoing complement generation in ventricular assist device XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity556 :: ('2147', 'Gene') :: ['Thrombin', 'thrombin'] @entity1423 :: ('MESH:D001791', 'Disease') :: ['leukocyte-platelet aggregates'] @entity4417 :: ('718', 'Gene') :: ['C3a'] @entity4461 :: ('MESH:D020141', 'Disease') :: ['hemostatic abnormalities']
@entity1 :: (9606,Species) :: ['patients']
