UNASSIGNED: @entity4742 ( @entity35937 ) is used as a kind of animal feed additive and affects @entity1 's health through the food chain. The purpose of this study is to detect the residue level of @entity35937 in 576 @entity1 urine samples directly. @entity35937 -BSA was used to immunize Balb/c mice. The monoclonal antibody was produced by hybridoma that was screened through cell fusion techniques. Finally, we developed the indirect competitive ELISA method to analyze 576 @entity1 urine samples from Zhejiang Province, China. The IC50 of this method was 3.33  ng/mL. The LOD and LOQ were 0.16 and 0.54  ng/mL. Linear range of the standard curve was from LOD to 12.50  ng/mL. There was no cross-reactivity with two kinds of @entity172 and two structural analogs with @entity35937 . Five hundred seventy-six urine samples were analyzed by the indirect competitive ELISA method, and the detection rate was 98.78%. The mean concentration and geometric mean were 4.70 and 3.50  ng/mL. The indirect competitive ELISA method based on monoclonal antibody was sensitive and reliable for the detection of @entity35937 in @entity1 urine samples. The results warned us to pay more attention to @entity1 health and food safety.

Investigation of @entity4742 residue level in XXXX urine samples by a specific monoclonal antibody.

@entity1 :: ('9606', 'Species') :: ['human', 'people'] @entity172 :: ('MESH:D004967', 'Chemical') :: ['estrogens'] @entity4742 :: ('MESH:D004054', 'Chemical') :: ['Diethylstilbestrol'] @entity35937 :: ('D004054', 'Chemical') :: ['DES']
@entity1 :: (9606,Species) :: ['human']
