In order to understand the regulatory mechanisms establishing and maintaining @entity29475 gene expression, structural information about the gene is a prerequisite. Therefore, we sequenced the 7.2-kb region of the @entity1 @entity29475 gene and mapped the positions of two partial cDNAs consisting of one of two 5' exons, AB (358 bp) or @entity1050 (568 bp), and a common 3' exon (exon II), which are separated by 5.4- and 1.0-kb introns, respectively. When the @entity535 sequence homologies were compared with those of other Hox genes belonging to the same paralogous group, exon @entity1050 exhibited the strongest homology: 73% of 91 aa residues exactly matched those of @entity1573 @entity69557 . An intermediate exon (90 bp) was detected within exon @entity1050 . It was surrounded by a splice acceptor and a donor at both the 5' and 3' ends, and one branchpoint site was found near the splice-acceptor site. Nucleotide sequence analysis along this region revealed two TATA boxes, one CAAT box, one GC box, and one each of the following binding sites--engrailed, eve-stripe2-hb3, and @entity32854 --just upstream of exon @entity1050 . A CpG island and two RARE repeats were detected within intron I. Northern blot analysis showed that at least four main transcripts were generated along this region: all fetal tissues tested (brain, lung, liver, and kidney) produced a 1.8-kb homeobox-containing transcript (HA-9A); a 2.2- and a 3.3-kb transcript were generated from exon @entity1050 and exon II (HA-9B), especially in fetal and adult kidneys as well as in adult skeletal muscle; the 1.0-kb transcript was likely to be generated by the intermediate exon in all adult and fetal tissues. Several weak bands without tissue specificity were likely to be contributed by the hybrid transcripts between @entity29475 and the other @entity42721 gene(s). Together, these results may account for the unique degree of conservation of the HOX cluster in general.

Genomic structure and sequence analysis of XXXX @entity29475 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity32854 :: ('1959', 'Gene') :: ['Krox20'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity29475 :: ('3205', 'Gene') :: ['HOXA-9'] @entity1050 :: ('MESH:D006223', 'Disease') :: ['CD'] @entity1573 :: ('9031', 'Species') :: ['chicken'] @entity69557 :: ('396096(Tax:9031)', 'Gene') :: ['Hoxa-9'] @entity42721 :: ('3197', 'Gene') :: ['HOXA']
@entity1 :: (9606,Species) :: ['human']
