CONTEXT: Differentiation between @entity652 and @entity1373 or even normal parathyroid tissue is difficult and based mainly on the surgeon's skill. Exploration of genes that express differentially in these various tissues using microarrays and other sophisticated research tools will enable identification and perhaps development of new methods of perioperative diagnosis. OBJECTIVE: To assemble a panel of kinase genes to differentiate @entity3243 from normal and @entity5072 parathyroid tissue. DESIGN: RNA was extracted from @entity652 , @entity1373 , and normal parathyroid tissue and hybridized to a microarray containing 359 @entity1 cDNAs of known kinase genes. Signals of exposure were scanned and quantified with software for digital image analysis. Semiquantitative reverse transcriptase polymerase chain reaction analysis of sample genes was performed, up-regulated or down-regulated, to validate the microarray results. RESULTS: The ratio values considered significant (<0.5 or >1.5) suggest that genes up-regulated in @entity3243 are those responsible for blood vessel angiogenesis and genes belonging to the cyclin-dependent kinase inhibitor groups. Genes down-regulated in @entity3243 are related to cellular growth and apoptosis--genes from the mitogen-activated protein kinase group and DNA-dependent protein kinase group. An interesting gene down-regulated in the @entity3243 samples is related to the @entity563 / @entity1889 protein kinases that exert a key function in @entity460 handling. A panel of 5 genes was defined: @entity42371 , @entity994 and the gene for @entity7 from the up-regulated group, and the gene for protein kinase C and @entity15188 from the down-regulated group. Reverse transcriptase polymerase chain reaction confirmed the microarray results for these genes. CONCLUSIONS: The kinase genes panel presented can be used to differentiate @entity3243 from normal and @entity5072 parathyroid tissue in particular when histopathology fails to provide a decisive diagnosis.

Differential expression of kinase genes in primary hyperparathyroidism: @entity652 versus normal and XXXX parathyroid tissue.

@entity1 :: ('9606', 'Species') :: ['human'] @entity7 :: ('7422', 'Gene') :: ['vascular endothelial growth factor'] @entity5072 :: ('MESH:D000796', 'Disease') :: ['hyperplastic'] @entity1373 :: ('MESH:D006965', 'Disease') :: ['hyperplasia'] @entity15188 :: ('6446', 'Gene') :: ['SGK'] @entity42371 :: ('1032', 'Gene') :: ['p19'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity563 :: ('CHEBI:17822', 'Chemical') :: ['serine'] @entity3243 :: ('MESH:D010282', 'Disease') :: ['parathyroid adenoma'] @entity652 :: ('MESH:D000236', 'Disease') :: ['adenoma'] @entity994 :: ('1026', 'Gene') :: ['p21'] @entity1889 :: ('CHEBI:26986', 'Chemical') :: ['threonine']
@entity5072 :: (MESH:D000796,Disease) :: ['hyperplastic']
