BACKGROUND: Exocytosis of eosinophil granule-derived mediators is thought to be an important effector response contributing to @entity32 . Secretion from many cell types has been shown to be dependent on the formation of a docking complex composed of soluble @entity8816 -sensitive factor attachment protein (SNAP) receptors (SNAREs) located on the vesicle (v-SNAREs) and the target membrane (t-SNAREs). The SNARE isoforms @entity10048 , @entity24835 , and @entity62676 have been described in secretory processes in myeloid cells. Previously, we have demonstrated that the @entity14442 @entity10048 is a candidate @entity14442 involved in eosinophil exocytosis and is localized to a pool of @entity5737 -positive vesicles that translocate to the cell periphery after @entity379 -induced degranulation. OBJECTIVE: We sought to determine whether eosinophils express the t-SNARE isoforms @entity24835 and @entity62676 as potential binding targets for @entity10048 during exocytosis. METHODS: @entity1 peripheral blood eosinophils (>97%) from atopic subjects were subjected to RT-PCR and sequence analysis by using specific primers for @entity24835 and @entity62676 . Protein expression and localization was determined by means of Western blot analysis of eosinophil subcellular fractions and confirmed with confocal laser scanning microscopy. RESULTS: @entity377 sequences obtained from PCR products exhibited nearly identical (>95%) homology with reported sequences for @entity1 @entity24835 and @entity62676 . Both @entity24835 and @entity62676 were present in plasma membranes, with some staining in endoplasmic reticulum and Golgi membranes. Negligible expression was detected in crystalloid and small secretory granules. CONCLUSIONS: The plasma membrane-associated t-SNAREs @entity24835 and @entity62676 are expressed in @entity1 eosinophils and are likely candidates for association with @entity10048 during docking, which is followed by exocytosis. These findings support a role for SNARE molecules in eosinophil mediator release.

Expression of eosinophil target SNAREs as potential cognate receptors for XXXX in exocytosis.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['allergic inflammation'] @entity5737 :: ('6352', 'Gene') :: ['RANTES'] @entity14442 :: ('10490', 'Gene') :: ['v-SNARE'] @entity24835 :: ('8773', 'Gene') :: ['SNAP-23'] @entity377 :: ('CHEBI:36976', 'Chemical') :: ['Nucleotide'] @entity8816 :: ('MESH:D005033', 'Chemical') :: ['N-ethylmaleimide'] @entity10048 :: ('6844', 'Gene') :: ['VAMP-2'] @entity379 :: ('3458', 'Gene') :: ['IFN-gamma'] @entity62676 :: ('6810', 'Gene') :: ['syntaxin-4']
@entity10048 :: (6844,Gene) :: ['vesicle-associated membrane protein-2']
