AIMS: @entity3396 is a ubiquitously expressed @entity460 -activated intracellular @entity462 protease. Altered expression of @entity262 has been implicated in @entity5 progression and response to chemotherapy. METHODS AND RESULTS: The aim of the current study was to confirm previous data that suggested that @entity3396 expression is associated with relapse-free survival in trastuzumab treated @entity0 @entity1 (n=93). An expanded @entity1 cohort from Nottingham was used (n=194; including 72 of the previous cohort), and an independent @entity1 cohort from Newcastle (n=87). All @entity1 received trastuzumab following adjuvant therapy according to local guidelines with expression of @entity3396 investigated using standard immunohistochemistry. Results show that @entity3396 expression is associated with relapse-free survival in both the Nottingham (P=0.01) and Newcastle (P=0.019) cohorts, with high expression associated with adverse relapse-free survival. Expression was also associated with poor relapse-free survival when @entity1 cohorts were combined (n=281, P=0.01). @entity3396 remained, from multivariate analysis, an independent marker for relapse-free survival in the Newcastle cohort (hazard ratio (HR) =5.169; 95% confidence interval (CI) =1.468-18.200; P=0.011). CONCLUSIONS: @entity3396 expression is associated with poor relapse-free @entity0 @entity1 treated with trastuzumab. Further work is warranted to standardise and develop methodology with a view to potentially introducing assessment of this important biomarker into clinical practice. This article is protected by copyright. All rights reserved.

XXXX is associated with adverse relapse free @entity0 : a confirmatory study.

@entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity3396 :: ('823', 'Gene') :: ['Calpain-1', 'calpain-1'] @entity462 :: ('MESH:D003545', 'Chemical') :: ['cysteine'] @entity0 :: ('MESH:D001943', 'Disease') :: ['survival in breast cancer', 'breast cancer'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity262 :: ('MESH:D034721', 'Disease') :: ['calpain system proteins'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium']
@entity3396 :: (823,Gene) :: ['Calpain-1']
