UNASSIGNED: @entity262 ( @entity262 ) is the most popular technology for @entity470 gene mutation analysis in China. Cutoff Ct or   Ct values were used to differentiate low mutation abundance cases from no mutation cases. In this study, all of 359 NSCLC samples were tested by @entity262 . Seventeen samples with larger Ct or   Ct than cutoff values were retested by PCR-sequencing. TKI treatment responses were monitored on the cases with @entity262 negative and PCR-sequencing positive results. One exon 18 @entity58183 case, 67 exon 19 deletion cases, 2 exon 20 insertion cases, 1 exon 20 @entity9513 case, 60 exon 21 @entity9512 cases, 5 exon 21 @entity20570 cases and 201 wild type cases were identified by @entity262 . Another 22 cases were evaluated as wild type but had later amplification fluorescent curves. Seventeen out of these 22 cases were retested by PCR-sequencing. It turns out that 3 out of 3 cases with exon 19 deletion later amplifications, 2 out of 2 cases with @entity9512 later amplifications and 4 out of 12 cases with @entity9513 later amplifications were identified as mutation positive. Two cases with exon 19 deletion and @entity9512 respectively were treated by TKI and got responses. Our study indicated that PCR-sequencing might be a complementary way to confirm @entity262 results with later amplifications.

PCR-sequencing is a complementary method to amplification refractory mutation system for XXXX gene mutation analysis in FFPE samples.

@entity470 :: ('1956', 'Gene') :: ['EGFR'] @entity20570 :: ('p|SUB|L|861|Q', 'ProteinMutation') :: ['L861Q'] @entity262 :: ('MESH:D034721', 'Disease') :: ['Amplification Refractory Mutation System', 'ARMS'] @entity9512 :: ('p|SUB|L|858|R', 'ProteinMutation') :: ['L858R'] @entity9513 :: ('p|SUB|T|790|M', 'ProteinMutation') :: ['T790M'] @entity58183 :: ('p|SUB|G|719|X', 'ProteinMutation') :: ['G719X']
@entity470 :: (1956,Gene) :: ['EGFR']
