The replicase protein nsP2 of @entity3597 ( @entity3598 ) has a 648RRR nuclear localization signal and is transported to the nucleus. @entity3598 -RDR has a single @entity535 change which disrupts this sequence and nsP2 nuclear transport. In BHK cells, @entity3599 and @entity3598 -RDR replicate to high titers, but @entity3598 -RDR is less virulent in @entity19 . We compared the replication of @entity3599 and @entity3598 -RDR in adult @entity19 brain. Both @entity3599 and @entity3598 -RDR were neuroinvasive following intraperitoneal inoculation. @entity3599 spread rapidly throughout the brain, whereas @entity3598 -RDR infection was confined to small foci of cells. Both viruses infected neurons and oligodendrocytes. Both viruses induced apoptosis in cultured BHK cells but not in the cells of the adult @entity19 brain. @entity3598 -RDR infection of @entity19 lacking alpha/beta interferon receptors resulted in widespread virus distribution in the brain. Thus, a component of the viral replicase plays an important role in the neuropathogenesis of @entity3598 .

A single XXXX change in the nuclear localization sequence of the nsP2 protein affects the neurovirulence of @entity3597 .

@entity3598 :: ('10271', 'Species') :: ['SFV'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity3599 :: ('338478', 'Species') :: ['SFV4'] @entity3597 :: ('11033', 'Species') :: ['Semliki Forest virus']
@entity535 :: (CHEBI:33704,Chemical) :: ['amino acid']
