UNASSIGNED: A semi-quantitative multiplex PCR assay for the diagnosis of @entity6981 ( @entity6981 ) was evaluated in a prospective study in a population of Dutch @entity1 with complaints of abnormal vaginal discharge. The PCR targets @entity8439 , Atopobium vaginae, Megasphaera phylotype 1, @entity10265 and @entity10265 iners. Together with a short questionnaire, a vaginal swab for PCR and vaginal smear for microscopy were taken by their general practitioner or gynaecologist. Data from 151 @entity1 (median age 32) were available. Nugent Score (NS) was used to classify the samples and 83 samples were classified as normal (NS 0-3), 13 as intermediate (NS 4-6), and 55 as @entity6981 (NS 7-10). In @entity1 with a NS of 7-10, PCR detected @entity8439 , Atopobium vaginae and Megasphaera phylotype 1 in respectively, 96  %, 87  % and 60  %, whereas in @entity1 with a NS of 1-3 these species were detected in 27  %, 6  % and 2  % (P <0.001). A ratio of @entity10265 over @entity10265 iners of <1 (as calculated from the quantification cycle value (Cq)) was present in @entity1 with a NS of 7-10 in 66  % versus 33  % in @entity1 with a NS of 1-3 (P <0.001). The @entity6981 -PCR displayed a sensitivity of 92  % and specificity of 96  % with a positive predictive value of 94  % and a negative predictive value of 95  %. The @entity10265 -index improved the correct classification of samples where only one of the other bacterial species was detected. Compared to the Nugent Score this multiplex qPCR offers a convenient tool for performing observer independent diagnosis of @entity6981 .

A multiplex real-time PCR assay for routine diagnosis of XXXX .

@entity1 :: ('9606', 'Species') :: ['women'] @entity8439 :: ('2702', 'Species') :: ['Gardnerella vaginalis'] @entity6981 :: ('MESH:D016585', 'Disease') :: ['BV', 'bacterial vaginosis'] @entity10265 :: ('47770', 'Species') :: ['Lactobacillus crispatus', 'Lactobacillus']
@entity6981 :: (MESH:D016585,Disease) :: ['bacterial vaginosis']
