BACKGROUND: MicroRNA (miRNA) expression is known to be deregulated in cervical @entity189 . However, no data is available about the miRNA expression pattern for the minimal deviation @entity420 ( @entity1865 ) of uterine cervix. We sought to detect deregulated miRNAs in @entity1865 in an attempt to find the most dependable miRNA or their combinations to understand their tumorigenesis pathway and to identify diagnostic or prognostic biomarkers. We also investigated the association between those miRNAs and their target genes, especially @entity125 and @entity126 . METHODS: We evaluated miRNA expression profiles via miRNA microarray and validated them using.real-time PCR assays with 24 @entity406 -fixed, @entity982 -embedded tissue blocks of @entity1865 and 11 normal proliferative endocervical tissues as control. Expression for @entity68343 was assessed by immunohistochemistry. RESULTS: MiRNA-135a-3p, 192-5p, 194-5p, and 494 were up-regulated, whereas miR-34b-5p, 204-5p, 299-5p, 424-5p, and 136-3p were down-regulated in @entity1865 compared with normal proliferative endocervical tissues (all P<0.05). Considering the second-order Akaike Information Criterion consisting of likelihood ratio and number of parameters, miR-34b-5p showed the best discrimination power among the nine candidate miRNAs. A combined panel of miR-34b-5p and 194-5p was the best fit model to discriminate between @entity1865 and control, revealing 100% sensitivity and specificity. @entity125 and @entity126 , respective target genes of miR-34b-5p and miR-204-5p, were more frequently expressed in @entity1865 than in control (63% vs. 18%; 52% vs. 18%, respectively, P<0.05). MiR-34b-5p expression level was higher in @entity125 -negative samples compared with @entity125 -positive ones (P<0.05). Down-regulated @entity20991 was associated with poor @entity1 survival (P=0.036). CONCLUSIONS: @entity1865 showed distinctive expression profiles of miRNAs, @entity125 , and @entity126 from normal proliferative endocervical tissues. In particular, miR-34b-5p and 194-5p might be used as diagnostic biomarkers and @entity20991 as a prognostic predictor for @entity1865 . The miR-34b-5p/ @entity125 pathway as well as @entity126 might be important oncogenic contributors to @entity1865 .

MicroRNA expression profiling and @entity125 and @entity126 expression in minimal deviation XXXX .

@entity20991 :: ('574452', 'Gene') :: ['miR-494'] @entity1 :: ('9606', 'Species') :: ['patient'] @entity1865 :: ('CHEBI:566274', 'Chemical') :: ['MDA'] @entity406 :: ('MESH:D005557', 'Chemical') :: ['formalin'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinomas'] @entity125 :: ('4851', 'Gene') :: ['Notch1'] @entity982 :: ('MESH:D010232', 'Chemical') :: ['paraffin'] @entity68343 :: ('4851;4853', 'Gene') :: ['Notch1 and 2'] @entity420 :: ('MESH:D000230', 'Disease') :: ['adenocarcinoma'] @entity126 :: ('4853', 'Gene') :: ['Notch2']
@entity420 :: (MESH:D000230,Disease) :: ['adenocarcinoma of uterine cervix']
