This study investigates the distribution of leucocytes, @entity3138 +, @entity404 +, @entity405 + lymphocyte subpopulations and MHC class II expressing cells in the sow endometrium following post-ovulatory insemination in relation to clinical findings and pregnancy outcome. Crossbred multiparous sows were inseminated once either at 15-20 h after ovulation [experiment 1, slaughtered at 20-25 h (5-6 h after artificial insemination (AI), group 1-A, n = 4), at 70 h after ovulation (group 1-B, n = 4), on day 11 (group 1-C, n = 4, first day of standing oestrus = day 1) or on day 19 (group 1-D, n = 4)] or 30 h after ovulation [experiment 2, slaughtered at 5-6 h after AI (group 2-A, n = 4) or on day 19 (group 2-D, n = 3)]. The uterine horns were flushed to control for the presence of @entity12179 and neutrophils and/or for recovery of oocytes and/or embryos. Mesometrial uterine samples were plastic embedded and stained. Cryofixed uterine samples were analysed by immunohistochemistry using mAbs to lymphocyte subpopulations and MHC class II molecules. Light microscopy was used to examine @entity11644 ( @entity11644 ) and glandular epithelia (GE), and connective tissue layers, both subepithelially (SL) and glandular (GL). In experiment 1, group 1-A, only one sow had @entity12179 in the utero-tubal junction (UTJ). Marked/moderated numbers of neutrophils and @entity12179 were observed in the flushings of two sows. In group 1-B, altogether 23 of 48 oocytes were cleaved. Day 11 (1-C), embryos with small diameter were observed. Day 19 (1-D), no embryos were found but small pieces of foetal membrane were observed in one of the sows. In group 1-A, large numbers of neutrophils were found within the @entity11644 and SL but with high individual variation. For T lymphocyte subpopulations, in the @entity11644 , most @entity3138 + cells were found in group 1-A. For both @entity11644 and GE in all groups, the number of @entity405 + cells was significantly larger than that of @entity404 + cells. In experiment 2, group 2-A, no sow had @entity12179 in the UTJ or in the uterine flushings. At day 19, no sow was pregnant. In group 2-A, large numbers of neutrophils were found within the @entity11644 and SL but with high individual variation. At day 19, high E2 levels showed a hormonal prooestrous stage but the endometrial neutrophil infiltration normally expected at pro-oestrus was absent. In conclusion, post-ovulatory insemination (about 18 h after ovulation) resulted in @entity73 . In sows post-ovulatory inseminated at a later stage (30 h after ovulation), no sow was pregnant. In both experiments, disturbed immune cell patterns were observed in some individuals.

Influence of post-ovulatory insemination on sperm distribution, pregnancy and the infiltration by cells of the immune system, and the distribution of @entity3138 , XXXX , @entity405 and MHC class II expressing cells in the sow endometrium.

@entity404 :: ('920', 'Gene') :: ['CD4'] @entity11644 :: ('MESH:C564120', 'Disease') :: ['SE', 'surface'] @entity73 :: ('MESH:D003072', 'Disease') :: ['impaired spermatozoa transport within the uterus and embryonic degeneration'] @entity3138 :: ('914', 'Gene') :: ['CD2'] @entity12179 :: ('OMIM:102530', 'Disease') :: ['spermatozoa'] @entity405 :: ('925', 'Gene') :: ['CD8']
@entity404 :: (920,Gene) :: ['CD4']
