UNASSIGNED: Fermented black @entity9052  (FBG) is processed by the repeated steaming and drying of fresh @entity9052 followed by fermentation with @entity375 . It is known to possess anti  -oxidative effects. Skin wrinkle formation is associated with oxidative stress and inflammatory reactions. The aim of this study was to determine whether FBG possesses anti  -wrinkle activity using @entity1 fibroblasts  (HS68). According to the Korea Ministry of Food and Drug Safety  (MFDS) guidelines for the evaluation of the efficacy of functional anti  -wrinkle cosmetics, we attempted to elucidate the effects of FBG on @entity4173 , @entity3334 , @entity1476 , @entity1495 and tissue inhibitor of metalloproteinase  -2  ( @entity1552 ). In addition, the eye irritation potential of FBG was examined using the EpiOcular  -EIT kit. Our results revealed that FBG was not cytotoxic at concentrations  <10    g/ml. It was considered as safe for the eyes at concentrations of up to 100    g/ml. Treatment with FBG at concentrations from 0.3  to 10    g/ml significantly  (P<0.05) increased the @entity4173 expression levels from 117.61  1.51 to 129.95  4.47% in the @entity1 fibroblasts. By contrast, FBG significantly  (P<0.05) decreased the @entity3334 expression level from 18.41  4.95 to 27.41  3.96%. FBG at 3    g/ml also increased the expression of @entity1552 up to  154.55%. However, FBG at 10    g/ml decreased the expression levels of @entity1476 and @entity1495 to 45.15  and  66.65%, respectively. These results suggest that FBG has potential anti  -wrinkle effects as a potential ingredient in cosmetics.

Anti  -wrinkle effect of fermented black XXXX on @entity1 fibroblasts.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1552 :: ('7077', 'Gene') :: ['TIMP  -2'] @entity375 :: ('4932', 'Species') :: ['Saccharomyces cerevisiae'] @entity4173 :: ('1278', 'Gene') :: ['type  I procollagen'] @entity3334 :: ('4312', 'Gene') :: ['matrix metalloproteinase  (MMP)  -1', 'MMP  -1'] @entity1476 :: ('4313', 'Gene') :: ['MMP  -2'] @entity1495 :: ('4318', 'Gene') :: ['MMP  -9'] @entity9052 :: ('4054', 'Species') :: ['ginseng']
@entity9052 :: (4054,Species) :: ['ginseng']
