The major autoantigens to anti-mitochondrial antibody (AMA) in @entity2835 ( @entity2835 ) have previously been identified to be PDC-E2, BCOADC-E2, and @entity19098 . However, analysis of these autoantigens to AMA cannot be examined using the two routine assays; immmunofluorescence and ELISA. Moreover, there are some problems in specificity and sensitivity in these routine assays. So, analysis with Western blotting using native mitochondrial protein as the antigen is required; it allows the identification of the molecular weights for the proteins which react with AMA in @entity1 ' sera. However, since the antigen-proteins used are not unified, molecular weights of AMA corresponding proteins vary among laboratories. In the present study, as the first step to help address this issue, we investigated the antigen specificity of protein bands detected by Western blotting using our in-house @entity291 and porcine heart mitochondrial proteins. Three major recombinant mitochondrial proteins were prepared. The antigen specificity was examined by the absorption tests preincubated with the three recombinant mitochondrial proteins. The molecular weights of developing our @entity291 and porcine heart mitochondrial proteins using @entity214 -PAGE were multiple protein bands including 74, 52, 50, and 43 kDa protein bands. Of them, the 74, 50, and 43 kDa protein bands were absorbed with preincubations of recombinant PDC-E2, BCOADC-E2, and @entity19098 protein, respectively. AMA specificity of these three major proteins with our Western blotting was confirmed.

Definition of antigen specificity for antimitochondrial proteins detected by Western blotting using native mitochondrial proteins in XXXX .

@entity2835 :: ('MESH:D008105', 'Disease') :: ['PBC', 'primary biliary cirrhosis'] @entity1 :: ('9606', 'Species') :: ['patients'] @entity19098 :: ('506888(Tax:9913)', 'Gene') :: ['OGDC-E2'] @entity291 :: ('9913', 'Species') :: ['bovine'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS']
@entity2835 :: (MESH:D008105,Disease) :: ['primary biliary cirrhosis']
