Frizzled related proteins (Frps) are secreted proteins structurally similar to frizzled receptors; they bind Wnt via the @entity462 -rich domain and antagonize the Wnt signaling pathway. In this study, we have investigated the mechanisms regulating the transcriptional regulation of @entity35 @entity69662 ( @entity69662 ) promoter. From previous findings, we know that the transcriptional activation domain of @entity69662 resides in the region -202 to -144 relative to the transcription start site, and that it is essential for efficient promoter activity. The study presented here was designed to identify trans-acting factors that bind to this critical domain of the @entity69662 promoter and to elucidate the pathway involved in the regulation of @entity69662 expression. Electrophoretic mobility shift assay (EMSA) demonstrated that specific DNA-protein binding activities fall into two adjacent core sequences with (CTTTGGGGG) at -197 to -189 and (AGATGATGTAA) at -151 to -141 of the @entity69662 promoter. Reporter assay showed that these core sequences are both required for the activation of @entity69662 promoter. Mutation within either one or both core sequence drastically reduced the promoter activity. Southwestern blotting showed that the estimated molecular mass of the distinct binding protein to the (AGATGATGTAA) domain is about 43 kDa. Further EMSA suggested @entity14889 as the trans-acting factor in the DNA-protein complex, which was out competed by @entity14889 consensus oligonucleotides and supershifted by anti- @entity14889 antibody. Overexpression of PKA and @entity14889 also transactivated @entity69662 promoter, and dominant-negative @entity14889 inhibited the promoter activity in transient reporter assays. More importantly, @entity14889 , phosphorylated @entity14889 and the adaptor protein @entity7052 were found binding to the endogenous @entity69662 promoter using chromatin immunoprecipitation assay. Collectively, our results demonstrate the induction of @entity69662 promoter activity by PKA and @entity14889 in vitro, and the binding of @entity14889 and @entity7052 to the @entity69662 promoter core motif in vivo.

Transcriptional regulation of the promoter of the @entity35 frizzled related protein gene by XXXX .

@entity462 :: ('MESH:D003545', 'Chemical') :: ['cysteine'] @entity69662 :: ('89803(Tax:10116)', 'Gene') :: ['Frp', 'rFrp'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity7052 :: ('54244(Tax:10116)', 'Gene') :: ['CBP'] @entity14889 :: ('81646(Tax:10116)', 'Gene') :: ['CREB']
@entity14889 :: (81646(Tax:10116),Gene) :: ['CREB']
