PURPOSE: To examine the effects of blocking @entity1995 mitogen-activated protein kinase (MAPK) on post-injury conjunctival @entity2318 in @entity19 . Its effects on the behaviors of cultured subconjunctival fibroblasts were also investigated. METHODS: An in vivo study was conducted using an adenoviral vector carrying a dominant-negative (DN)- @entity1995 gene. A circumferential incision was made in the equatorial conjunctiva by scissors in the right eye of generally anesthetized adult C57BL/6 @entity19 . DN- @entity1995 -expressing adenoviral vector was topically applied. The left control eye received non-functioning adenoviral vector. At 2, 5, and 7 days (each, n=22) the eyes were processed for histological or immunohistochemical examination to evaluate the tissue @entity2318 . The expressions of type-I collagen and growth factors were evaluated by real time-reverse transcriptase-polymerase chain reaction. The effects of @entity1995 inhibitor on the proliferation, @entity4222 , and fibrogenic gene/protein expression of cultured @entity1 fibroblasts were also studied. RESULTS: The in vivo DN- @entity1995 gene introduction blocked the phospho- @entity1995 expression with reduction of myofibroblast generation and suppression of mRNA expression of @entity1612 ( @entity1612 ) and @entity1750 ( @entity1750 ) in the @entity19 -injured conjunctiva. Blocking @entity1995 signal in the fibroblasts by a chemical inhibitor counteracted @entity641 's enhancement of expressions of type-I collagen, fibronectin, and @entity1612 . It also retarded cell @entity4222 , but cell proliferation was unchanged. CONCLUSIONS: Inhibiting @entity1995 signal impairs the fibrogenic reaction induced by the subconjunctival fibroblasts in vivo and in vitro, suggesting its potential effectiveness in preventing excessive @entity2318 following @entity726 filtering surgery.

Inhibition of p38MAP kinase suppresses fibrogenic reaction in conjunctiva in XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity1750 :: ('20296(Tax:10090)', 'Gene') :: ['monocyte/macrophage chemoattractant protein-1', 'MCP-1'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity2318 :: ('MESH:D002921', 'Disease') :: ['scarring'] @entity4222 :: ('MESH:D014085', 'Disease') :: ['migration'] @entity641 :: ('21803(Tax:10090)', 'Gene') :: ['TGFbeta1'] @entity1612 :: ('14219(Tax:10090)', 'Gene') :: ['CTGF', 'connective tissue growth factor'] @entity726 :: ('MESH:D005901', 'Disease') :: ['glaucoma'] @entity1995 :: ('26416(Tax:10090)', 'Gene') :: ['p38MAPK', 'p38']
@entity19 :: (10090,Species) :: ['mice']
