BACKGROUND: @entity13236 is produced by certain T helper cells subsets (Th17, Th22) and at lower levels by y-   T cells, NKT and innate lymphoid cells. Th22 cells are unique immune cells that regulate tissue responses by @entity13236 production. The exact discrimination between Th17 cells that co-produce @entity13236 and single @entity13236 -producing Th22 cells has not been possible until the present study. Isolation of pure Th22 cells without co-expression of cytokines of other T-cell subsets is essential to better understand their function in @entity1 . The aim of this study is the isolation and characterization of viable, @entity1 @entity13236 -producing CD4+ T cells that do not produce @entity4155 . METHODS: Isolation of viable Th22 cells was performed with the combination of two cytokine secretion assays detecting @entity4155 - and @entity13236 -producing cells in a single purification step. RESULTS: The newly developed cytokine secretion assay consists of anti- @entity13236 and anti- @entity4155 catch antibodies, which via @entity1885 -streptavidin interaction are bound to the biotinylated surface of the target cell, and anti- @entity13236 and @entity4155 detection antibody labelled with a fluorescent dye, which detects cytokines bound to these catch antibodies. A unique population of @entity1 Th22 cells, which do not produce @entity4155 , was sorted, and cytokine expression pattern was confirmed by quantitative PCR analysis and ELISA. The presented technique allows the detection and isolation of pure @entity1 Th22 cells. CONCLUSIONS: This technique may allow the purification of any single cytokine-producing cell subset, and the combination of several different cytokine secretion assays can be used to purify and characterize novel and unique cell subsets.

A novel, dual cytokine-secretion assay for the purification of XXXX Th22 cells that do not co-produce @entity4155 .

@entity13236 :: ('50616', 'Gene') :: ['IL-22', 'Interleukin-22'] @entity1 :: ('9606', 'Species') :: ['humans', 'human'] @entity4155 :: ('3605', 'Gene') :: ['IL-17A'] @entity1885 :: ('MESH:D001710', 'Chemical') :: ['biotin']
@entity1 :: (9606,Species) :: ['human']
