UNASSIGNED: As a possible marker of oxidative stress, many studies have measured whole blood reduced @entity2041 ( @entity6172 ) and @entity9492 ( @entity9492 ). However, large differences in @entity6172 and @entity9492 levels reported in different studies, calls for a reliable standardized method. In this study, we validate not only analytical performance of new measurement method for @entity6172 and @entity9492 , but also the clinical utility of these markers in a @entity19 model with chronic oxidative stress. Twenty @entity19 were randomized into four treatment groups according to @entity68 burden: 0mg, 5mg, 10mg, or 15mg of @entity68 were injected into the peritoneum per day over 4 weeks. To prevent artifactual @entity6172 auto-oxidation, we pretreated the sample with @entity8816 ( @entity17462 ) immediately after sample collection. After protein precipitation using @entity44254 , @entity9492 and @entity6172 - @entity17462 were measured using liquid chromatography-tandem mass spectrometry (LC- @entity15 / @entity15 ). The mean @entity6172 / @entity9492 ratios of the @entity19 model were 163.1, 31.0, 27.9, and 12.8 for control, 5mg, 10mg, and 15mg injection groups, respectively, showing a decrease in the @entity6172 / @entity9492 ratios according to the amount of oxidative stress induced. Inter-assay coefficients of variation were 4.1% for @entity6172 - @entity17462 and 7.3% for @entity9492 . Recoveries were 98.0-105.9% for @entity6172 - @entity17462 and 98.0-107.3% for @entity9492 . No ion suppression was observed at the retention time for @entity6172 - @entity17462 and @entity9492 . This study suggests an accurate method that can be used for @entity2041 measurement using LC- @entity15 / @entity15 , and showed that @entity6172 / @entity9492 ratio could provide an assessment of the degree of oxidative stress.

Validation of a liquid chromatography tandem mass spectrometry method to measure oxidized and reduced forms of @entity2041 in whole blood and verification in a XXXX model as an indicator of oxidative stress.

@entity9492 :: ('MESH:D019803', 'Chemical') :: ['GSSG', 'oxidized glutathione'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity44254 :: ('MESH:C003366', 'Chemical') :: ['sulfosalicylic acid'] @entity2041 :: ('MESH:D005978', 'Chemical') :: ['glutathione'] @entity8816 :: ('MESH:D005033', 'Chemical') :: ['N-ethylmaleimide'] @entity6172 :: ('D005978', 'Chemical') :: ['GSH'] @entity15 :: ('MESH:D009103', 'Disease') :: ['MS'] @entity17462 :: ('CHEBI:44485', 'Chemical') :: ['NEM'] @entity68 :: ('MESH:D007501', 'Chemical') :: ['iron']
@entity19 :: (10090,Species) :: ['mouse']
