Expression of @entity14357 ( @entity14357 ), one member of an inducible @entity563 / @entity1889 kinase family, is induced by FSH/ @entity268 in @entity35 granulosa cells cultured in defined medium. The FSH-stimulated pattern of @entity14357 expression is biphasic, and transcriptional activation of the @entity14357 gene depends on an intact Sp1/Sp3 binding site within the proximal promoter. To determine whether @entity14357 was expressed in a hormone-dependent and physiologically relevant manner in vivo, the cellular levels of @entity14357 messenger RNA (mRNA) and protein as well as the subcellular localization of this kinase were analyzed in ovaries containing follicles and corpora lutea at specific stages of differentiation. To stimulate follicular development and luteinization, hypophysectomized ( @entity5698 were treated with @entity53 (E; HE) and FSH (FSH; HEF) followed by hCG (hCG; HEF/hCG). To analyze @entity14357 in functional corpora lutea, PRL was administered to HEF/hCG @entity35 , or ovaries of pregnant @entity35 were obtained on day 7, 15, or 22 of gestation. In situ hybridization indicated that @entity14357 mRNA was low/undetectable in granulosa cells of H and HE @entity35 . An acute injection (i.v.) of FSH to HE @entity35 rapidly increased @entity14357 mRNA at 2 and 8 h. @entity14357 mRNA was also elevated in granulosa cells of preovulatory follicles of HEF @entity35 and in luteal cells of HEF/hCG and pregnant @entity35 . Northern blots and Western blots confirmed the in situ hybridization data, indicating that the amount and cellular localization @entity14357 protein were related to that of @entity14357 mRNA. When the subcellular localization of this kinase was analyzed by immunohistochemistry, @entity14357 protein was nuclear in granulosa cells and some thecal cells of large preovulatory follicles. In contrast, @entity14357 protein was cytoplasmic in luteal cells as well as some cells within the stromal compartment. Intense immunostaining was also observed in oocytes present in primordial follicles, but not in growing follicles. Collectively, these results show that FSH and LH stimulate marked increases in the cellular content of @entity14357 , as well as dramatic changes in the subcellular distribution of this kinase. The specific nuclear vs. cytoplasmic compartmentalization of @entity14357 in granulosa cells and luteal cells, respectively, indicates that @entity14357 controls distinct functions in proliferative vs. terminally differentiated granulosa cells.

Expression and localization of serum/glucocorticoid-induced kinase in the XXXX ovary: relation to follicular growth and differentiation.

@entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity14357 :: ('29517(Tax:10116)', 'Gene') :: ['serum/glucocorticoid-inducible kinase', 'sgk', 'Sgk'] @entity5698 :: ('10117', 'Species') :: ['H) rats'] @entity53 :: ('MESH:D004958', 'Chemical') :: ['estradiol'] @entity563 :: ('CHEBI:17822', 'Chemical') :: ['serine'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity1889 :: ('CHEBI:26986', 'Chemical') :: ['threonine']
@entity35 :: (10116,Species) :: ['rat']
