UNASSIGNED: A species D human adenovirus ( @entity43799 ) isolated from diarrhoeal faeces of an allogenic haematopoietic @entity8 ( @entity8 ) recipient was found to be non typeable by sequencing of loops 1 and 2 of the hexon main neutralization epitope ("imputed serology"). In contrast to species @entity42649 , @entity43799 infections are rarely observed in @entity8 @entity1 . Therefore, the whole genome of this isolate was sequenced and phylogenetically analysed. In addition, micro neutralisation testing with type specific antisera was performed. A complete genomic sequence of 35.2 kb in length with a GC content of 57% was obtained and found to be distantly related to HAdV-D27 (96.25% identity). Imputed serology implicated a new type with a nucleotide sequence identity of only 96.11% to HAdV-D37 (loop 1) and 95.76% to HAdV-D30 and -D37 (loop 2). Micro neutralisation testing confirmed that this clinical isolate was not neutralised by HAdV-D37 or -D30 specific antisera. The penton base gene showed a novel sequence clustering with HAdV-D38, but bootscan analysis indicated an intra-penton recombination event with HAdV-D60. Another recombination event was detected within the early genes region E3 with the 12.2kDa and the CR1-alpha genes derived from HAdV-D58. Moreover, the E4 region was derived from HAdV-D13 but all these genes had evolved significantly from their ancestors. By contrast, the recombinant fiber gene was almost 100% identical to HAdV-D29. In conclusion, the genomics of this novel @entity1 adenovirus, designated as the HAdV-D70 [P70H70F29] prototype, supported the significance of multiple recombinations in the phylogeny of species D human adenoviruses.

XXXX Adenovirus type 70: A novel, multiple recombinant species D adenovirus isolated from diarrheal faeces of a haematopoietic stem cell transplantation recipient.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity42649 :: ('129951', 'Species') :: ['HAdV-C'] @entity8 :: ('MESH:D007674', 'Disease') :: ['SCT', 'stem cell transplant'] @entity43799 :: ('130310', 'Species') :: ['HAdV-D']
@entity1 :: (9606,Species) :: ['Human']
