UNASSIGNED: This study aimed to investigate the role of miRNAs in HPV-mediated cervical @entity5 and @entity5 cases in Indian population. We analysed the @entity281 and its genotypes in uterine cervical @entity5 (n   =   80), @entity5 (n   =   200) and normal cervical samples (n   =   150) by consensus sequence PCR followed by type specific PCRs. Also, microRNA profiling was done in a subset of cervical @entity5 (n   =   20), @entity5 cases (n   =   50) and normal samples (n   =   30) by real-time quantitative PCR (qRT-PCR). The prevalence of @entity281 in @entity5 was found to be 81  % (65/80) and 94  % (188/200) in @entity5 cases, with most predominant high-risk HPV @entity1799 (HR- @entity1799 ) in 83  % of @entity5 and 91  % of pre- @entity5 cases, respectively. Whereas in controls, the @entity281 was found to be very low (5  %). The miRNA profiling revealed that in cervical @entity5 , 100 miRNAs were significantly (p   <   0.001) differentially expressed with 70 miRNAs upregulated and 30 miRNAs downregulated. In @entity5 cases, 383 miRNA were found to be differentially expressed (p   <   0.001), of which 350 miRNAs were upregulated and 33 miRNAs were downregulated. We also observed that 182 miRNAs were differentially expressed (p   <   0.001) in @entity1799 /18-positive ( @entity1 /HeLa) cell lines compared with HPV-negative ( @entity1 ) cell line. In addition, we identified the novel microRNAs such as @entity69701 , @entity43357 , @entity69702 , @entity35482 and @entity69703 in cervical @entity1613 and @entity5 cases in Indian population. Taken together, the study demonstrates a crucial role of microRNAs in @entity5 , which may serve as potential early diagnostic markers for cervical @entity105 .

Novel MicroRNA signatures in HPV-mediated cervical @entity105 in Indian XXXX .

@entity1 :: ('9606', 'Species') :: ['SiHa', 'C33A'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer', 'pre-cancer', 'cervical cancer'] @entity281 :: ('MESH:D007239', 'Disease') :: ['HPV infection'] @entity35482 :: ('574508', 'Gene') :: ['miR-505'] @entity1799 :: ('333760', 'Species') :: ['type-16', 'HPV-16'] @entity1613 :: ('MESH:D011230', 'Disease') :: ['precancerous lesions'] @entity69703 :: ('100313843', 'Gene') :: ['miR-711'] @entity43357 :: ('574502', 'Gene') :: ['miR-500'] @entity105 :: ('MESH:D063646', 'Disease') :: ['carcinogenesis'] @entity69701 :: ('100126307', 'Gene') :: ['miR-892b'] @entity69702 :: ('100126306', 'Gene') :: ['miR-888']
@entity1 :: (9606,Species) :: ['women']
