BACKGROUND: Integrins participate in cell-cell and cell-matrix interactions. In this study we determined whether alphaII(b)beta3 integrin is involved in @entity3 of @entity1 @entity263 cells. METHODS: @entity263 @entity5049 and DU-145 cell lines express alphaII(b)beta3. Northern blotting, 5'-RACE, and immunofluorescent localization confirmed expression of alphaIIb integrin in @entity263 cells. We used orthotopic/ectopic site of implantation and lung colonization assays in @entity915 @entity19 to determine whether alphaII(b)beta3 participates in metastatasis of @entity5 cells. RESULTS: Immunofluorescent localization of alphaIIb integrin in fibronectin-adherent DU-145 and @entity5049 cells is remarkably different. In DU-145 cells the integrin localizes to focal contact sites, whereas it is predominantly intracellular in @entity5049 cells. Both @entity5 cell lines are tumorigenic when implanted subcutaneously or intraprostatically in @entity915 @entity19 , but only DU-145 cells injected intraprostatically metastasize. Flow cytometry with a mAb directed to alphaII(b)beta3 revealed higher expression of alphaII(b)beta3 in DU-145 @entity5 cell suspensions isolated from the prostate when compared to DU-145 @entity5 cells from the subcutis. Function-blocking mAbs to alphaII(b)beta3 inhibit lung colonization of tail vein-injected DU-145 cells. CONCLUSIONS: Altogether, the data suggest that alphaII(b)beta3 integrin participates in the metastatic progression of @entity263 .

Role of alphaII(b)beta3 integrin in prostate cancer XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity263 :: ('MESH:D011471', 'Disease') :: ['prostate adenocarcinoma', 'prostatic adenocarcinoma', 'Prostate adenocarcinoma'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity915 :: ('MESH:D053632', 'Disease') :: ['SCID'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity5049 :: ('57332', 'Gene') :: ['PC-3']
@entity3 :: (MESH:D009362,Disease) :: ['metastasis']
