The @entity4910 gene, localized to chromosome band 11q13, is amplified in approximately 15% of @entity1 primary @entity0 . From 30 to 40% of the @entity5 presenting this amplification show concomitant amplification at the @entity7167 locus in 8p12. Similarly, @entity0 cells bear @entity4910 and @entity7167 coamplified, resulting in the formation of a hybrid intrachromosomal amplification assembling 11q13 and 8p12 sequences. To learn whether similar amplified structures arise in @entity0 , we used a two-color FISH approach on interphase nuclei. A cohort of 225 @entity0 was analyzed by Southern blotting and a subset of 12 @entity5 presenting the 11q13-8p12 coamplification was selected for further study by interphase FISH. In 6/12 @entity5 the FISH signals for 11q13 and 8p12 probes formed colocalizing clusters of green and red spots in the nuclei. The FISH patterns were identical to those observed on MDA-MB-134 interphase nuclei hybridized with 11q13 and 8p12. These data, suggesting the formation in these @entity5 of a hybrid amplification domain in which 11q13 and 8p12 sequences are joined, were reinforced by dual-color FISH on extended chromatin showing that the said were sequentially aligned in these @entity5 . Furthermore, 3/6 nuclei with colocalized 11q13 and 8p12 amplifications showed fusion of centromeric sequences from chromosomes 8 and 11. Our data strongly suggest the occurrence, in approximately 3% of primary @entity0 , of a recurrent rearrangement involving the proximal portions of 8p and 11q and resulting in the formation of a hybrid amplified structure composed of 11q13 and 8p12 sequences.

@entity4910 and @entity7167 coamplification results in the colocalization of 11q13 and 8p12 sequences in XXXX nuclei.

@entity1 :: ('9606', 'Species') :: ['human'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast tumors', 'MDA-MB-134 breast cancer'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumors'] @entity4910 :: ('595', 'Gene') :: ['CCND1'] @entity7167 :: ('2260', 'Gene') :: ['FGFR1']
@entity0 :: (MESH:D001943,Disease) :: ['breast tumor']
