To investigate simultaneously localization and relative activity of MMPs during extracellular matrix (ECM) remodeling in @entity1732 -induced @entity3794 in @entity35 , we analyzed the time course of the expression, activity and/or concentration of gelatinases @entity11017 and @entity6929 , collagenase @entity17533 , matrylisin @entity20742 , @entity6930 and @entity11018 , both in alveolar space (cellular and extracellular compartments) and in lung tissue. MMP and @entity6930 expression was detected (immunohistochemistry) in lung tissue. MMP activity (zymography) and @entity6930 concentration (ELISA) were evaluated in lung tissue homogenate (LTH), BAL supernatant (BALs) and BAL cell pellet (BALp) 3, 7, 14, and 28 days after @entity1732 intratracheal instillation. Immunohistochemistry showed an extensive MMP and @entity6930 expression from day 7 in a wide range of structural and inflammatory cells in treated @entity35 . @entity11017 was present mainly in epithelia, @entity6929 in inflammatory cells. @entity11017 and @entity6929 activity was increased respectively in BAL fluid and BAL cells, with a peak at day 7. @entity6930 and @entity11018 concentration (ELISA) enhancement was delayed at day 14. In conclusion gelatinases and their inhibitors are significantly activated during @entity1732 -induced @entity3794 . Marked changes in gelatinases activity are observed early in the alveolar compartment, with a prevailing extracellular activity of @entity11017 and a predominant intracellular distribution of @entity6929 , while enzyme activity changes in lung parenchyma were less evident. In the repairing phase the reduction of gelatinases activity is synchronous with a peak of alveolar concentration of their inhibitors.

Time course of matrix metalloproteases and tissue inhibitors in XXXX -induced @entity3794 .

@entity17533 :: ('300339(Tax:10116)', 'Gene') :: ['MMP-1'] @entity6930 :: ('116510(Tax:10116)', 'Gene') :: ['TIMP-1', 'TIMP'] @entity3794 :: ('MESH:D011658', 'Disease') :: ['pulmonary fibrosis'] @entity11018 :: ('29543(Tax:10116)', 'Gene') :: ['TIMP-2'] @entity6929 :: ('81687(Tax:10116)', 'Gene') :: ['MMP-9'] @entity1732 :: ('MESH:D001761', 'Chemical') :: ['bleomycin'] @entity20742 :: ('25335(Tax:10116)', 'Gene') :: ['MMP-7'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity11017 :: ('81686(Tax:10116)', 'Gene') :: ['MMP-2']
@entity1732 :: (MESH:D001761,Chemical) :: ['bleomycin']
