UNASSIGNED: To minimize the side effects and enhance the efficiency of chemotherapy, a novel folate-decorated hydrophilic cationic star-block terpolymer, [poly(l-glutamic acid y-hydrazide)-b-poly(N,N-dimethylaminopropyl methacrylamide)]3-g-poly(ethylene glycol) ((PGAH-b-PDMAPMA)3-g-PEG), with disulfide linkages between the PEG and PDMAPMA blocks, was developed for targeted co-delivery of doxorubicin and @entity588 small interfering RNA (siRNA) into @entity0 cells. The terpolymer was synthesized by a combination of ring-opening polymerization, reversible addition-fragmentation chain transfer polymerization, PEGylation and hydrazinolysis. The chemical structures of the polymers were confirmed by (1)H-NMR analysis. The terpolymer could conjugate doxorubicin via an acid-labile hydrazone linkage and simultaneously efficiently complex siRNA through electrostatic interaction at N/P ratios of    4:1 to form "two-in-one" nanomicelleplexes, which displayed a spherical shape and had an average size of 101.3nm. The doxorubicin loading efficiency and content were 61.0 and 13.23%, respectively. The @entity137 , drug release profile, targeting ability, cellular uptake and intracellular distribution of the nanomicelleplexes were evaluated in vitro. We found that the release behaviors of doxorubicin and siRNA had a pH/reduction dual dependency. They were released faster under reductive acidic conditions (pH 5.0, glutathione: 10mM) than under physiological conditions (pH 7.4). The folate-decorated nanomicelleplexes could deliver doxorubicin and @entity588 siRNA more efficiently into the same MCF-7 cell and exhibited a higher @entity137 than non-targeted nanomicelleplexes. These results indicate that the terpolymer could act as an efficient vehicle for targeted intracellular co-delivery of doxorubicin and therapeutic siRNA in @entity5 therapy.

Folate-decorated hydrophilic three-arm star-block terpolymer as a novel nanovehicle for targeted co-delivery of doxorubicin and XXXX siRNA in @entity0 therapy.

@entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity588 :: ('596', 'Gene') :: ['Bcl-2'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer']
@entity588 :: (596,Gene) :: ['Bcl-2']
