CONTEXT: Immunohistochemical staining for @entity4166 ( @entity4166 ) is a useful ancillary technique for diagnosing @entity3557 ( @entity1377 ) in histologic specimens. Although similar results have been obtained on aspirate smears, to our knowledge the utility of @entity4166 immunolocalization in cell block preparations as an adjunct to fine-needle aspiration diagnosis of @entity1377 has not been examined. OBJECTIVE: The purpose of this study was to determine whether @entity4166 immunostaining of cell block preparations of thyroid aspirates is a useful ancillary technique for diagnosing @entity1377 . MATERIALS AND METHODS: Using a monoclonal antibody to @entity4166 and a standard avidin- @entity1885 complex technique, immunostaining was performed on paraffin-embedded cell blocks of 57 cases with the following cytologic diagnoses: (a) @entity1377 (20 cases); (b) atypical cytology, cannot exclude @entity1377 (19 cases); and (c) nonneoplastic thyroid (18 cases). The staining reaction in each case was graded on the basis of percentage of epithelial cells stained (1+, <10%; 2+, <10%-50%; 3+, >50). Tissue follow-up was available in all cases. RESULTS: Nineteen (95%) of 20 cases with an unequivocal diagnosis of @entity1377 were positive for @entity4166 (3+). Tissue follow-up confirmed @entity1377 in all 20 cases. Of the 19 cases with a diagnosis of atypical cytology, cannot exclude @entity1377 , 7 (37%) cases displayed 3+ immunostaining and subsequent excision confirmed @entity1377 in all 7 cases. The remaining 12 cases with 1+ immunostaining included surgically confirmed @entity5651 (6 cases), @entity652 (2 cases), @entity1669 (3 cases), and @entity1377 (1 case). The follicular cells in 18 cases with a cytologic diagnosis of nonneoplastic thyroid showed 1+ immunostaining. Histologic follow-up of these cases confirmed the nonneoplastic cytologic diagnoses. CONCLUSIONS: @entity4166 immunostaining of cell block preparations of thyroid aspirates serves as a useful tool for the diagnosis of @entity1377 . Strong immunostaining (3+) for @entity4166 aids in accurate diagnosis of @entity5 in cytomorphologically equivocal cases of @entity1377 .

XXXX immunolocalization in cell block preparation of thyroid aspirates. An adjunct to fine-needle aspiration diagnosis of @entity3557 .

@entity3557 :: ('MESH:C536915', 'Disease') :: ['papillary thyroid carcinoma'] @entity1377 :: ('MESH:D002291', 'Disease') :: ['papillary carcinoma'] @entity4166 :: ('3880', 'Gene') :: ['Cytokeratin 19', 'cytokeratin 19', 'CK-19'] @entity1885 :: ('MESH:D001710', 'Chemical') :: ['biotin'] @entity1669 :: ('MESH:D013967', 'Disease') :: ['lymphocytic thyroiditis'] @entity5 :: ('MESH:D009369', 'Disease') :: ['malignancy'] @entity5651 :: ('MESH:D006042', 'Disease') :: ['goiter'] @entity652 :: ('MESH:D000236', 'Disease') :: ['adenoma']
@entity4166 :: (3880,Gene) :: ['Cytokeratin 19']
