BACKGROUND/AIMS: @entity5 cells are resistant to @entity63 and starvation. @entity11 -associated oncogene homolog 1 ( @entity8889 ) is a positive transcriptional activator of Hedgehog (Hh) pathway and plays an essential role in the development of @entity5 , including @entity0 . However, how @entity8889 promotes cell survival remains elusive. The main purpose of this study is to investigate the pro-survival effect of @entity8889 under serum starvation and its molecular mechanism in ER-positive @entity0 cells. METHODS: Gene expression was determined by quantitative real-time PCR (QRT-PCR) and Western blot. The survival of @entity8889 stably transfected ER-positive @entity0 cell lines ( @entity8889 -MCF-7 and @entity8889 -T47D cells) and their untransfected control cells was estimated by WST-8 assay. Microarray analysis was performed to screen downstream Hh/ @entity8889 target genes in @entity8889 -overexpressed MCF-7 cells. Transcriptional activities of NF-kappaB were measured by luciferase assays. ChIP analysis was performed to explore whether @entity1837 was a direct target gene of @entity8889 . RESULTS: Serum starvation significantly up-regulated the expression of @entity8889 gene through activating PI3K/AKT pathway. Over-expression of @entity8889 markedly promoted cell survival under serum starvation. Microarray analysis revealed that 338 genes were differentially expressed in @entity8889 -MCF-7 cells compared with those in the control cells. Among these genes, @entity1837 ( @entity1837 ), coding an anti-apoptosis and pro-survival protein, was significantly up-regulated not only by Hh/ @entity8889 pathway, but also by serum starvation. However, ChIP assay revealed no binding of @entity8889 to @entity1837 promoter at the region of -1792 to -1568bp. Moreover, over-expression of @entity8889 resulted in enhanced trans-activation of transcriptional factor NF-kB. Suppression of NF-kB signaling with NF-kB inhibitor @entity30462 , significantly reduced the expression of @entity1837 and the cell survival under serum starvation. CONCLUSION: Serum starvation significantly up-regulated the expression of @entity8889 , which in turn increased its key target @entity1837 expression and enhanced NF-kB/ @entity1837 pathway, resulting in promoting cell survival under serum starvation. These findings may provide new insights into the pro-survival mechanisms of @entity8889 in @entity0 .    2015 S. Karger AG, Basel.

Up-Regulation of @entity11 -Associated Oncogene Homolog 1 Expression by Serum Starvation Promotes Cell Survival in ER-Positive XXXX Cells.

@entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemia'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancers', 'Cancer'] @entity1837 :: ('330', 'Gene') :: ['cIAP2', 'cellular inhibitor of apoptosis 2'] @entity8889 :: ('2735', 'Gene') :: ['Gli1'] @entity11 :: ('MESH:D005910', 'Disease') :: ['Glioma'] @entity30462 :: ('MESH:C434003', 'Chemical') :: ['Bay11-7082']
@entity0 :: (MESH:D001943,Disease) :: ['Breast Cancer']
