Blastocyst culture requires strictly defined culture media to sustain its viability and quality. Although blastocyst media are commercially available, they do not meet all the needs and research focused on blastocyst-promoting agents is on the way. The aims of the study were to evaluate the significance of @entity7424 ( @entity7424 ) and II ( @entity10247 ); @entity6354 ( @entity6354 ) and a mixture of insulin, @entity3654 and @entity4259 (ITS) on the development of embryos exposed to oxidative stress. C3B6F1 @entity19 were stimulated with 5 IU of pregnant mare serum gonadotropin following by administration of 5 IU of @entity1641 chorionic gonadotropin and mating with @entity7840 males. The @entity19 were killed 40 h after eCG injection by cervical dislocation and then the 2 cell embryos were flushed out from the fallopian tubes. To evaluate whether the growth factors may compensate the unfavorable--oxidative milieu created by @entity273 ( @entity274 ), the embryos were transferred to 1/ control medium, 2/ control medium+0.1 mM ( @entity274 ) or 3/ control medium+ @entity274 enriched with 10(-7) g/ml of @entity7424 , @entity10247 , @entity6354 or a mixture of insulin (5x10(-6) g/ml), @entity3654 (5 x10(-6) g/ml) and @entity4259 (5x10(-9) g/ml; ITS). Embryos were evaluated 96-144 hours following eCG injection. In the study the dynamics of embryo development and blastocyst cell numbers (including inner cell mass) were assessed. The morphological evaluation comprised viability and apoptosis (TUNEL). In oxidative stress setting, @entity7424 , @entity10247 , @entity6354 and ITS minimized the negative influence of @entity274 , and embryos developed faster than in control conditions. Blastocysts cultured with @entity273 and growth factors or ITS displayed normal morphology and had more cells--also within the inner cell mass--than those treated only with @entity274 . The positive TUNEL reactions were sporadically observed in embryos cultured with @entity273 supplemented with growth factors. @entity7424 , @entity10247 , @entity6354 and ITS have a positive effect on pre-implantation embryo development in detrimental culture conditions of oxidative stress.

Evaluation of @entity19 preimplantation embryos exposed to oxidative stress cultured with insulin-like growth factor I and II, @entity6354 , insulin, @entity3654 and XXXX .

@entity1641 :: ('9796', 'Species') :: ['equine'] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H2O2'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity4259 :: ('MESH:D012643', 'Chemical') :: ['selenium'] @entity6354 :: ('13645(Tax:10090)', 'Gene') :: ['EGF', 'epidermal growth factor'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['hydrogen peroxide'] @entity10247 :: ('16002(Tax:10090)', 'Gene') :: ['IGF-II'] @entity7424 :: ('16000(Tax:10090)', 'Gene') :: ['insulin-like growth factors I', 'IGF-I'] @entity3654 :: ('22041(Tax:10090)', 'Gene') :: ['transferrin'] @entity7840 :: ('CHEBI:35299', 'Chemical') :: ['DBA']
@entity4259 :: (MESH:D012643,Chemical) :: ['selenium']
