@entity1110 -oxidase (NOX)-dependent reactive @entity26 species (ROS) production is involved in self-renewal of stem and progenitor cells. Herein, we investigated whether high @entity413 (25 mM/L) (HG)-dependent NOX-mediated ROS generation is involved in self-renewal of visceral adipose tissue-derived stem cells (ASCs) as well. To this end ASCs cultured in HG or normal @entity413 (5 mM/L) used as control, were evaluated for their stem cell identity. We demonstrated that freshly isolated ASCs are pluripotent as they differentiate into adipocytes in-vitro and form neovessels in-vivo. However, only HG-cultured ASCs expressed @entity4729 ( @entity4729 ) and @entity2440 and formed spheroids. The assembly of @entity20995 and @entity20994 subunits is crucial for NOX-enzymatic activity. By knock-down of @entity1114 phox the role of NOX-generated ROS in driving @entity938 de-differentiation has been provided. siRNA technology was also applied to demonstrate the role of @entity584 activity in mediating HG-induced @entity4729 and @entity2440 expression as well as spheroid formation. Additionally, by knock-down of @entity4729 we provided further evidence that @entity4729 is essential for HG-mediated stem cell identity. Soluble factors released by ASCs are key elements in their mechanism of action. We found that NOX and @entity584 activity are required for cytokine production by "spheroids". Finally, as HG-cultured ASCs, @entity6 @entity1 -derived ASCs expressed higher levels of @entity4729 and @entity2440 than ASCs derived from healthy subjects and engaged ROS and @entity584 activity to turn on their secretion program. Thereby, our data indicate that HG via NOX-dependent @entity584 activity induces @entity938 de-differentiation, and suggest that HG pre-conditioning might be exploited for @entity938 ex-vivo expansion.

High XXXX via NOX-dependent ROS generation and @entity584 activity promotes adipose-derived stem cell de-differentiation.

@entity1 :: ('9606', 'Species') :: ['patient'] @entity20995 :: ('653361', 'Gene') :: ['p47phox'] @entity4729 :: ('5460', 'Gene') :: ['Oct4', 'octamer-binding transcription factor 4', 'Oct-4'] @entity2440 :: ('79923', 'Gene') :: ['Nanog'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetic'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity584 :: ('207', 'Gene') :: ['Akt'] @entity1110 :: ('MESH:D009249', 'Chemical') :: ['NADPH'] @entity1114 :: ('5341', 'Gene') :: ['p47'] @entity938 :: ('29108', 'Gene') :: ['ASC'] @entity20994 :: ('4688', 'Gene') :: ['p67phox'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity413 :: (MESH:D005947,Chemical) :: ['glucose']
