BACKGROUND: Tracheal intubation of laboratory @entity19 remains essential yet challenging for most researchers. The aim of this study was to investigate whether this procedure can be more efficiently and safely accomplished by a novel method using slim and torqueable guidewires to guide access to the @entity825 . METHODS: This study was carried out in an animal laboratory affiliated to a tertiary medical center. @entity19 weighing 22 to 28  g were subjected to various open-chest experiments after being anesthetized with intraperitoneal @entity3652 (100  mg/kg) and @entity3303 (10  mg/kg). The oropharyngeal cavity was opened with angled tissue forceps, and the @entity825 was transilluminated using an external light. The vocal cords were then crossed using either the Conventional method with a 38-mm-long, end-blunted stiff needle as a guide for insertion of a 22-gauge, 25-mm-long intravenous catheter into the @entity825 , or the Modified method utilizing using a 0.014-inch-thin torqueable wire as the guide to introduce an identical tube over it into the @entity825 . The epithelial integrity of the @entity825 was later examined histologically when the animals were sacrificed either immediately after the surgery or at 28  days post-surgery, depending on the corresponding research protocols. RESULTS: Orotracheal intubation was successfully completed in all @entity19 using either the Conventional (N   =   42) or the Modified method (N   =   50). With the Modified method, intubation took less time (1.73 vs. 2.17  min, Modified vs. Conventional, p   <   0.001) and fewer attempts (1.0 vs. 1.33, p   <   0.001), and there were fewer procedural difficulties (0  % vs. 16.7  %, p   =   0.009) and complications (0  % vs. 11.9  %, p   =   0.041) compared with the Conventional method. Histological analysis revealed a significantly lower incidence of immediate (0  % vs. 39  %, p   <   0.001) and late (0  % vs. 58  %, p   <   0.001) injuries to the tracheal epithelial lining with the Modified method compared to the Conventional method. CONCLUSIONS: Tracheal intubation for laboratory @entity19 can be completed efficiently, safely and atraumatically using the proposed Modified method employing readily available inexpensive instruments.

Efficacious and safe orotracheal intubation for laboratory XXXX using slim torqueable guidewire-based technique: comparisons between a modified and a conventional method.

@entity19 :: ('10090', 'Species') :: ['mice', 'Mice'] @entity3303 :: ('MESH:D008012', 'Chemical') :: ['lidocaine hydrochloride'] @entity825 :: ('MESH:D055090', 'Disease') :: ['trachea'] @entity3652 :: ('MESH:D007649', 'Chemical') :: ['ketamine']
@entity19 :: (10090,Species) :: ['mice']
