OBJECTIVE: The aim of this study is to investigate the sensitivity change and the preliminary mechanism of @entity43 cells on the resistant to chemotherapeutic drugs by inhibiting @entity21176 expression. METHODS: The @entity43 cell lines A2780 was administrated with antagomir-23a and @entity902 , and then the cell proliferation inhibition rate was determined by @entity98 assay. The cell cycle distribution was detected by flow cytometric analysis. The apoptotic morphological changes were analyzed by @entity2455 staining. The glycoprotein @entity7895 expression changes were detected by Western blot analysis. RESULTS: The cell proliferation inhibition rate increased significantly after the administration of @entity21176 and @entity902 (P<0.01). The middle concentration of drug efficacy IC50 in experimental group decreased by 83.76% compared with that in control group, which was 17.89  mol/L vs 110.18  mol/L (P<0.01). The cell lines A2780 were arrested in G0/G1 phase and apoptosis rate kept increasing (P<0.05). The cell nuclei stained by @entity2455 were obviously enhanced and demonstrated apoptosis morphology, such as condense, pyknosis. Compared with control group, the levels of @entity7895 protein expression in experimental group decreased along with the increase of the @entity900 concentration (P<0.05). CONCLUSION: The inhibition of @entity21176 expression could significantly increase the sensitivity of @entity900 towards @entity5 cells, and it was probably because the negative regulatory factors of @entity21176 target genes was released, and as a result, the expression of @entity7895 protein was inhibited.

Molecular mechanism of increased sensitivity of XXXX to @entity43 by inhibition of microRNA-23a expression.

@entity43 :: ('MESH:D010051', 'Disease') :: ['ovarian cancer'] @entity2455 :: ('MESH:D006690', 'Chemical') :: ['Hoechst33258'] @entity21176 :: ('407010', 'Gene') :: ['miR-23a'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity900 :: ('MESH:D002945', 'Chemical') :: ['cisplatin'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity7895 :: ('283871', 'Gene') :: ['P-gp'] @entity902 :: ('MESH:D010984', 'Chemical') :: ['platinum']
@entity900 :: (MESH:D002945,Chemical) :: ['cisplatin']
