The extraction and quantitation of @entity6738 metabolites from cell lysate was accomplished using a @entity6738 -reactive fluorous tag and capillary liquid chromatography coupled to mass spectrometry (capLC- @entity15 ). Selective fluorous tagging for @entity16623 and @entity3125 provided a 30-fold increase in sensitivity using electrospray ionization @entity15 . Separation of fluorous tagged @entity6738 resulted in good separation of all components, and tandem @entity15 was able to differentiate structural @entity6738 isomers. The average limit of detection for @entity6738 standards was 37 nM (range 1.5-250 nM), with linearity of R(2) > 0.99. Reproducibility for metabolites in cell lysate averaged 9% RSD. @entity1 aortic endothelial cells (HAECs) were exposed to varying levels of @entity413 , and their @entity6738 metabolite levels were quantified. Significant metabolite changes were seen in glycolysis and the propanoate pathway from a @entity413 challenge. Using an untargeted approach, 120 @entity6738 metabolites were found to change in @entity1602 HAECs. From this list of compounds, multiple metabolites from the @entity36435 and @entity6635 metabolic pathways were discovered. This system provides excellent sensitivity and quantitation of @entity6738 metabolites without the need for isotope standards or labels.

Extraction and Quantitation of @entity16623 and XXXX from @entity1 Cells Using Fluorous Tagging and @entity15 .

@entity1 :: ('9606', 'Species') :: ['Human'] @entity6635 :: ('D014364', 'Chemical') :: ['tryptophan'] @entity16623 :: ('MESH:D007659', 'Chemical') :: ['ketones'] @entity1602 :: ('MESH:D006944', 'Disease') :: ['hyperglycemic'] @entity3125 :: ('MESH:D000447', 'Chemical') :: ['aldehydes'] @entity36435 :: ('D010428', 'Chemical') :: ['pentose phosphate'] @entity15 :: ('MESH:D009103', 'Disease') :: ['MS'] @entity6738 :: ('CHEBI:23019', 'Chemical') :: ['carbonyl'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity3125 :: (MESH:D000447,Chemical) :: ['Aldehydes']
