UNASSIGNED: Although cell-mediated immunity based diagnostics can be integral assays for early detection of various diseases of dairy @entity291 , processing of blood samples for these tests is time-sensitive, often within 24 hours of collection, to maintain white blood cell viability. Therefore, to improve utility and practicality of such assays, the objective of this study was to assess the use of a novel white blood cell preservation technology in whole @entity291 blood. Blood samples from ten healthy @entity291 were each divided into an unpreserved control sample and a test sample preserved with commercially-available cell transport medium. Samples were maintained at room temperature and stimulated with the mitogens pokeweed and concanavalinA, as well as with interleukin-12 p40. Stimulation was completed on days 1, 5, and 8 post-sampling. Viability of white blood cells was assessed through @entity12028 production determined with a commercial enzyme linked immunosorbent assay. In addition, mononuclear cell viability was assessed with @entity2667 flow cytometry. Greater @entity12028 production was observed on days 5 and 8 post-collection in preserved samples, with both pokeweed and concanavalinA stimulating positive @entity12028 production on day 5 post-collection. A greater proportion of the amount of @entity12028 produced on day 1 continued to be produced on days 5 and 8 post-collection with concanavalinA stimulation (with or without interleukin 12) as compared to pokeweed stimulation. Additionally, viable mononuclear cells were still present at eight days post-collection, with a higher mean proportion detected at days 5 and 8 in all stimulated preserved samples. This practical and simple method to extend in vitro white blood cell viability could benefit the efficient utilization of cell-based blood tests in ruminants.

Novel Cell Preservation Technique to Extend XXXX In Vitro White Blood Cell Viability.

@entity291 :: ('9913', 'Species') :: ['bovine', 'cows'] @entity12028 :: ('281237(Tax:9913)', 'Gene') :: ['interferon gamma'] @entity2667 :: ('MESH:D011419', 'Chemical') :: ['propidium iodide']
@entity291 :: (9913,Species) :: ['Bovine']
