Radiation therapy is a cornerstone of modern @entity5 treatment. Understanding the mechanisms behind normal tissue sensitivity is essential in order to minimize adverse side effects and yet to prevent @entity5 reoccurrence. The aim of this study was to identify biomarkers of radiation sensitivity to enable personalized @entity5 treatment. To investigate the mechanisms behind radiation sensitivity a pilot study was made where eight radiation-sensitive and nine normo-sensitive @entity1 were selected from a cohort of 2914 @entity0 @entity1 , based on acute tissue reactions after radiation therapy. Whole blood was sampled and irradiated in vitro with 0, 1, or 150mGy followed by 3h incubation at 37  C. The leukocytes of the two groups were isolated, pooled and protein expression profiles were investigated using isotope-coded protein labeling method (ICPL). First, leukocytes from the in vitro irradiated whole blood from normo-sensitive and extremely sensitive @entity1 were compared to the non-irradiated controls. To validate this first study a second ICPL analysis comparing only the non-irradiated samples was conducted. Both approaches showed unique proteomic signatures separating the two groups at the basal level and after doses of 1 and 150mGy. Pathway analyses of both proteomic approaches suggest that oxidative stress response, coagulation properties and acute phase response are hallmarks of radiation sensitivity supporting our previous study on oxidative stress response. This investigation provides unique characteristics of radiation sensitivity essential for individualized radiation therapy.

Unique proteomic signature for radiation sensitive @entity1 ; a comparative study between normo-sensitive and radiation sensitive @entity0 XXXX .

@entity5 :: ('MESH:D009369', 'Disease') :: ['cancer', 'local cancer'] @entity1 :: ('9606', 'Species') :: ['patients'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer']
@entity1 :: (9606,Species) :: ['patients']
