UNASSIGNED: Present study was designed to develop novel nano-structured lipid carriers (NLCs) formulated by lipid mixture and chemical permeation enhancer-based hydrogel for an effective transdermal delivery of @entity1499 ( @entity6822 ). The prepared NLCs were optimized with different preparative variables such as particle size <200nm, poly-dispersity index (PDI) <0.2, and entrapment efficiency    85%. The drug incorporated into NLCs-gel base showed excellent spread ability without any grittiness during rheological behavior and texture profile analysis. The in vitro release showed biphasic release pattern with initial fast release of drug (>50%) in 8h followed by sustained release (up to 85%) by the end of 48thh. NLCs showed greater uptake in @entity1 hyper-proliferative keratinocyte cell line (HaCaT). NLCs showed increased expression of inflammatory mediators as well asapoptosis in U937 monocytic cells. The greater expression of pro-apoptotic gene Bim regulated by NF-kB-IkB and @entity4012 is supported by fold regulations calculated for various apoptotic and pro-inflammatory biomarkers carried out by RT-PCR. The immunocytochemistry to detect @entity650 expression and immunofluorescence assay suggested that induced apoptosis occurs in experimentally induced in vitro @entity625 model treated with NLCs- @entity6822 . We saw reduced @entity32 and triggered apoptosis through NF-kB _ @entity4012 pathways induced by @entity6822 loaded NLCs in rheumatoid arthritic cells. In addition, formulated NLCs exhibit better skin permeation with higher permeation flux _ enhancement ratio as shown by confocal laser scanning microscopy (CLSM). Moreover, histopathological examinations of skin are suggestive of safety potential of NLCs.

Nanostructured lipid carrier mediates effective delivery of XXXX to induce apoptosis of rheumatoid arthritis via NF-kB and @entity4012 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity650 :: ('3569', 'Gene') :: ['IL-6'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity625 :: ('MESH:D001168', 'Disease') :: ['arthritis'] @entity6822 :: ('D008727', 'Chemical') :: ['MTX'] @entity4012 :: ('2308', 'Gene') :: ['FOXO1'] @entity1499 :: ('MESH:D008727', 'Chemical') :: ['methotrexate']
@entity1499 :: (MESH:D008727,Chemical) :: ['methotrexate']
