OBJECTIVE: To assess the effect of two @entity3457 ( @entity3457 ) protocols with the use of two @entity460 ( @entity141 ) ionophores, @entity1360 and @entity3365 (calcimycin), on the intracellular @entity141 level in @entity19 and @entity1 oocytes and the fertilization rates. DESIGN: Comparison of two @entity141 ionophores, @entity1360 and @entity3365 , regarding their capacity to increase the intracellular @entity141 level and to support subsequent oocyte activation and development. SETTING: University hospital research laboratory. @entity1 (S)/ANIMAL(S): @entity1 undergoing intracytoplasmic sperm injection (ICSI) treatment and B6D2F1 @entity19 . INTERVENTION(S): Assisted oocyte activation and microinjection of @entity19 and @entity1 oocytes with sperm. MAIN OUTCOME MEASURE(S): Measurement of the fertilizing and @entity141 -releasing ability of @entity1 sperm. RESULT(S): @entity1360 was more potent than @entity3365 in provoking @entity141 increases in both @entity19 and @entity1 oocytes with significantly higher amplitude and area under the receiver operating characteristic curve. The oocyte activation rate was significantly higher when @entity19 oocytes were activated with the use of the @entity1360 - rather than the @entity3365 -based @entity3457 protocol. Furthermore, oocyte activation rate was higher when @entity1 in  vitro matured oocytes were activated with the @entity1360 -based @entity3457 protocol, but the difference did not reach statistical significance. CONCLUSION(S): In both @entity19 and @entity1 oocytes, the @entity3457 protocol that used @entity1360 was more efficient than the one that used @entity3365 . Bearing in mind that @entity1 fertilization is successful when the total dose of @entity141 released reaches a minimal threshold, the use of @entity1360 for @entity1 @entity3457 might be justified instead of the use of @entity3365 .

Effect of two XXXX protocols used to overcome fertilization failure on the activation potential and @entity460 releasing pattern.

@entity1 :: ('9606', 'Species') :: ['mammalian', 'Patients', 'human', 'PATIENT'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity1360 :: ('MESH:D015759', 'Chemical') :: ['Ionomycin', 'ionomycin'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity3365 :: ('MESH:D000001', 'Chemical') :: ['A23187'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity3457 :: ('OMIM:612348', 'Disease') :: ['AOA', 'assisted oocyte activation']
@entity3457 :: (OMIM:612348,Disease) :: ['assisted oocyte activation']
