The median survival of @entity1 with @entity463 ( @entity463 ) remains poor. Innovative immunotherapies with dendritic cell (DC) vaccination might be combined with standard @entity2903 ( @entity2904 ) treatment. Here, we evaluated the influence of @entity2904 on the phenotype and function of DCs and CD8+ T cells. DCs were generated from the peripheral blood of healthy volunteers (HVs) and @entity463 @entity1 . DCs were analyzed by light microscopy and flow cytometry. Phagocytic activity was tested by @entity2823 -dextran engulfment. Mixed lymphocyte peptide cultures were followed by enzyme-linked immunospot (ELISPOT) and flow cytometry assays. @entity2904 was added to DC and T cell cultures at concentrations up to 500   M. Mature DCs were generated from HVs and @entity463 @entity1 . Cells displayed a typical DC morphology and a mature DC phenotype. Expression of @entity20120 was even higher in DCs generated from @entity1 under therapy than from HVs (75.2 vs. 51.1%). In contrast, @entity2936 (1.1 vs. 13.5%) and @entity12867 (26.5 vs. 52.9%) were lower expressed in @entity463 @entity1 at time of diagnosis. Immature DCs showed high phagocytic activity. Addition of @entity2904 at concentrations up to 50   M did neither impair the phenotype nor the function of DCs. In ELISPOT and flow cytometry assays, no impairment of CD8+ T cell responses to viral antigens could be observed. Taken together, @entity2904 does not impair the function of either DCs or the CD8+ T cells.

Therapeutical doses of XXXX do not impair the function of dendritic cells and CD8+ T cells.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity2936 :: ('958', 'Gene') :: ['CD40'] @entity20120 :: ('30835', 'Gene') :: ['CD209'] @entity2823 :: ('MESH:D016650', 'Chemical') :: ['FITC'] @entity2903 :: ('MESH:C047246', 'Chemical') :: ['temozolomide'] @entity2904 :: ('CHEBI:72564', 'Chemical') :: ['TMZ'] @entity463 :: ('MESH:D005909', 'Disease') :: ['glioblastoma multiforme', 'GBM'] @entity12867 :: ('8829', 'Gene') :: ['BDCA4']
@entity2903 :: (MESH:C047246,Chemical) :: ['temozolomide']
