Although nearly one fifth of all @entity1 @entity5 have an infectious aetiology, the causes for the majority of @entity5 remain unexplained. Despite the enormous data output from high-throughput shotgun sequencing, viral DNA in a clinical sample typically constitutes a proportion of host DNA that is too small to be detected. Sequence variation among @entity778 genomes complicates application of sequence-specific, and highly sensitive, PCR methods. Therefore, we aimed to develop and characterize a method that permits sensitive detection of sequences despite considerable variation. We demonstrate that our low-stringency in-solution hybridization method enables detection of <100 viral copies. Furthermore, distantly related proviral sequences may be enriched by orders of magnitude, enabling discovery of hitherto unknown viral sequences by high-throughput sequencing. The sensitivity was sufficient to detect retroviral sequences in clinical samples. We used this method to conduct an investigation for novel retrovirus in samples from three @entity5 types. In accordance with recent studies our investigation revealed no @entity281 in @entity1 @entity1892 cells, cutaneous @entity784 or @entity14 biopsies. Nonetheless, our generally applicable method makes sensitive detection possible and permits sequencing of distantly related sequences from complex material.

Investigation of XXXX Cancers for Retrovirus by Low-Stringency Target Enrichment and High-Throughput Sequencing.

@entity1 :: ('9606', 'Species') :: ['human'] @entity778 :: ('MESH:D015658', 'Disease') :: ['virus'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancers', 'cancer'] @entity784 :: ('MESH:D016399', 'Disease') :: ['T-cell lymphoma'] @entity281 :: ('MESH:D007239', 'Disease') :: ['retroviral infections'] @entity1892 :: ('MESH:D016393', 'Disease') :: ['B-cell lymphoma'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal cancer']
@entity1 :: (9606,Species) :: ['Human']
