@entity1028 ( @entity1028 ) is associated with increased endothelin ( @entity2232 ), both systemically and locally in the aorta. Also, elastase activity is increased in @entity1 @entity1028 , and elastase perfusion of the aorta induces @entity439 formation in animal models of @entity1028 . However, whether elastase directly affects the @entity2232 -induced mechanisms of aortic smooth muscle contraction is unclear. Isometric contraction and 45Ca2+ influx were measured in aortic strips isolated from male @entity35 and treated with elastase (5 U/mL). To avoid degradation of the extracellular matrix proteins by elastase, experiments were performed in the presence of @entity2362 (10 mg/mL). In normal Krebs solution (2.5 mmol/L @entity141 ), @entity2232 (10(-7) mol/L) caused contraction of aortic strips that was inhibited by elastase (5 U/mL). The elastase-induced inhibition of @entity2232 contraction was slow in onset (4.6+/-0.4 minutes), time-dependent, complete in 34+/-3 minutes, and reversible. In @entity141 -free Krebs solution, @entity5834 (25 mmol/L) caused a small contraction that was not inhibited by elastase, suggesting that elastase does not inhibit @entity141 release from the intracellular stores. Membrane depolarization by 96 mmol/L @entity4338 , which stimulates @entity141 entry from the extracellular space, caused a contraction that was inhibited by elastase in a concentration-dependent, time-dependent, and reversible fashion. The reversible inhibitory effects of elastase, particularly in the presence of @entity2362 , suggest that they are not due to dissolution of the extracellular matrix or smooth muscle contractile proteins. Elastase also inhibited @entity2232 and @entity4338 -induced 45Ca2+ influx. Thus, elastase directly inhibits @entity2232 -induced @entity141 entry mechanisms of vascular smooth muscle contraction, which may explain the role of elastase and @entity2232 during the development of @entity1028 .

Elastase-induced suppression of endothelin-mediated XXXX entry mechanisms of vascular contraction.

@entity1 :: ('9606', 'Species') :: ['human'] @entity2362 :: ('2006', 'Gene') :: ['elastin'] @entity2232 :: ('1906', 'Gene') :: ['ET-1'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+'] @entity5834 :: ('MESH:D002110', 'Chemical') :: ['caffeine'] @entity439 :: ('MESH:D000783', 'Disease') :: ['aneurysm'] @entity35 :: ('10116', 'Species') :: ['Sprague-Dawley rats'] @entity1028 :: ('MESH:D017544', 'Disease') :: ['Abdominal aortic aneurysm', 'AAA'] @entity4338 :: ('CHEBI:32588', 'Chemical') :: ['KCl']
@entity141 :: (CHEBI:29108,Chemical) :: ['Ca2+']
