@entity5929 ( @entity5929 ), occurring primarily in @entity1 with @entity565 or @entity1577 ( @entity1577 ), is a hypersensitivity reaction to @entity54 (Af), and is characterized by increased serum IgE levels and peripheral blood and @entity5930 . We evaluated the IgE and cytokine profile in @entity5929 through enzyme-linked immunosorbent assay (ELISA), and evaluated eosinophil activity with the @entity29074 ( @entity29074 ) assay. IgE and cytokines were measured in supernatants from cultures of peripheral blood mononuclear cells (PBMC) from three subject groups: @entity5929 @entity1 , @entity1 with @entity565 , and healthy individuals. All cultures for the three subject groups were studied in the presence and absence of two purified Af antigens (the 35-kD antigen and @entity1457 protein 1). We found that increased in vitro levels of IgE in unstimulated PBMC culture supernatants correlated significantly with serum IgE concentrations in @entity5929 @entity1 . We measured a decrease in IgE levels of up to 75% of baseline values in supernatants from PBMC cultured with Af antigens. @entity1199 ( @entity1199 ) and @entity379 ( @entity379 ) concentrations in cultures with Af were increased in @entity5929 , whereas concentrations of @entity3646 did not differ in the three subject groups. An inverse relation was noted between the changes in IgE and @entity379 measured in 4 of 5 @entity5929 @entity1 . The PBMC supernatants also promoted @entity29074 activity in purified eosinophils from @entity5929 @entity1 , and to a lesser extent in purified eosinophils from healthy subjects. These results show that the 35-kD antigen and @entity70410 from Af downregulate IgE in vitro but are capable of inducing @entity1712 in @entity5929 . Further studies could result in the characterization of epitopes leading to these disparate effects. An identification of the IgE-down-regulating epitopes in Af antigens might have therapeutic significance.

IgE down regulation and cytokine induction by XXXX antigens in @entity1 allergic bronchopulmonary aspergillosis.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity1712 :: ('MESH:D004802', 'Disease') :: ['eosinophilia'] @entity29074 :: ('8288', 'Gene') :: ['eosinophil peroxidase', 'EPO'] @entity565 :: ('MESH:D001249', 'Disease') :: ['asthma'] @entity1457 :: ('MESH:D012769', 'Disease') :: ['heat shock'] @entity5930 :: ('MESH:D011657', 'Disease') :: ['pulmonary eosinophilia'] @entity70410 :: ('6856', 'Gene') :: ['HSP1'] @entity54 :: ('746128', 'Species') :: ['Aspergillus fumigatus'] @entity5929 :: ('MESH:D001229', 'Disease') :: ['Allergic bronchopulmonary aspergillosis', 'ABPA'] @entity379 :: ('3458', 'Gene') :: ['IFN-gamma', 'interferon-gamma'] @entity1199 :: ('3558', 'Gene') :: ['IL-2', 'Interleukin-2'] @entity3646 :: ('3565', 'Gene') :: ['IL-4'] @entity1577 :: ('MESH:D003550', 'Disease') :: ['CF', 'cystic fibrosis']
@entity54 :: (746128,Species) :: ['Aspergillus']
