Golli proteins, products of the @entity10927 gene, function as a new type of modulator of intracellular @entity141 levels in oligodendrocyte progenitor cells (OPCs). Because of this, they affect a number of @entity141 -dependent functions, such as OPC migration and process extension. To examine further the @entity141 channels regulated by golli, we studied the store-operated @entity141 channels (SOCCs) in OPCs and acute brain slice preparations from golli knock-out and golli-overexpressing @entity19 . Our results showed that pharmacologically induced @entity141 release from intracellular stores evoked a significant extracellular @entity141 entry after store depletion in OPCs. They also indicated that, under these pharmacological conditions, golli promoted activation of @entity141 influx by SOCCs in cultured OPCs as well as in tissue slices. The canonical transient receptor potential family of @entity141 channels (TRPCs) has been postulated to be SOCC subunits in oligodendrocytes. Using a small interfering RNA knockdown approach, we provided direct evidence that @entity27097 is involved in store-operated @entity141 influx in OPCs and that it is modulated by golli. Furthermore, our data indicated that golli is probably associated with @entity27097 at OPC processes. Additionally, we found that @entity27097 expression is essential for the effects of golli on OPC proliferation. In summary, our data indicate a key role for golli proteins in the regulation of TRPC-mediated @entity141 influx, a finding that has profound consequences for the regulation of multiple biological processes in OPCs. More important, we have shown that extracellular @entity141 uptake through @entity27097 is an essential component in the mechanism of OPC proliferation.

Modulation of canonical transient receptor potential channel 1 in the proliferation of oligodendrocyte precursor cells by the golli products of the XXXX gene.

@entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity10927 :: ('17196(Tax:10090)', 'Gene') :: ['myelin basic protein'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity27097 :: ('22063(Tax:10090)', 'Gene') :: ['TRPC1']
@entity10927 :: (17196(Tax:10090),Gene) :: ['myelin basic protein']
