BACKGROUND: Studies in @entity1 have shown that haemostatic and inflammatory pathways both play important roles in the pathogenesis of @entity2720 . The aim of this study was to assess mRNA expression of haemostatic and inflammatory factors in cultured @entity1641 fibroblast-like synoviocytes exposed to lipopolysaccharide (LPS), fibrinogen and thrombin. Synovial membranes were collected from metacarpo-phalangeal joints of 6 skeletally mature @entity1641 euthanized for non-orthopaedic reasons. Passage 4 fibroblast-like synoviocytes were left non-treated or treated with either 0.1   g/ml LPS, 5  mg/ml fibrinogen or 5 U/ml thrombin and harvested at time points 0, 6, 24 and 48  h. mRNA expression of @entity36621 ( @entity36621 ), @entity10661 ( @entity10661 ), @entity70481 ( @entity70481 ), tissue factor (TF), @entity70482 ( @entity70482 ), urokinase plasminogen activator (uPA), vascular endothelial growth factor (VEGF) and protease activator receptor 1 (PAR-1) was assessed using quantitative real time reverse transcriptase PCR. RESULTS: LPS caused a significant increase in mRNA expression of @entity36621 , @entity10661 , @entity70481 and uPA, and a decrease in TF, @entity70482 and PAR-1 when compared to non-treated cells. Treatment with thrombin resulted in increased mRNA expression of @entity36621 , @entity10661 , @entity70481 and @entity70482 , and a decreased PAR-1 expression compared to non-treated cells. The fibrinogen-treated synoviocytes showed significantly increased mRNA expression of @entity10661 , @entity70481 , TF and @entity70482 , and decreased PAR-1 expression compared to non-treated cells. CONCLUSION: LPS, fibrinogen and thrombin induced an increased gene expression of inflammatory markers in isolated @entity1641 fibroblast-like synoviocytes. LPS caused changes in gene expression promoting increased fibrinolysis, while fibrinogen and thrombin changed the gene expression resulting potentially in reduced fibrinolysis. Overall, it appeared that both inflammatory and haemostatic stimuli affected expression of genes involved in inflammatory and haemostatic pathways, supporting their importance in @entity1641 @entity2720 .

mRNA expression of genes involved in inflammation and haemostasis in XXXX fibroblast-like synoviocytes following exposure to lipopolysaccharide, fibrinogen and thrombin.

@entity1 :: ('9606', 'Species') :: ['humans'] @entity70481 :: ('100034136(Tax:9796)', 'Gene') :: ['monocyte chemotactic protein 1', 'MCP-1'] @entity1641 :: ('9796', 'Species') :: ['horses', 'equine'] @entity10661 :: ('100034196(Tax:9796)', 'Gene') :: ['IL-6', 'interleukin-6'] @entity36621 :: ('100033905(Tax:9796)', 'Gene') :: ['serum amyloid A', 'SAA'] @entity2720 :: ('MESH:D007592', 'Disease') :: ['joint diseases', 'joint disease'] @entity70482 :: ('100033931(Tax:9796)', 'Gene') :: ['PAI-1', 'plasminogen activator inhibitor 1']
@entity1641 :: (9796,Species) :: ['equine']
