Enzyme-catalyzed synthesis of @entity69052 is reported. It is the first example of direct esterification of @entity13266 which provides good yields. The reaction was performed in a heterogeneous medium by Candida rugosa lipase powder suspended in a @entity4315 / @entity8336 mixture. The impact of some factors was examined. @entity13266 does not inhibit the lipase until 100 mM. Above 90 mM, @entity1042 inhibits the enzyme. This inhibition is partially eliminated by increasing @entity13266 concentration. Below 90 mM, @entity1042 mainly interacts with the water adsorbed on the biocatalyst. A minimum water content is necessary to activate the biocatalyst. Water must be provided proportionally to the lipase content. Toluene, necessary for @entity13266 solubilization, also acts negatively on reaction kinetics. This is attributed to a modification of @entity13266 partition between the biocatalytic and the organic phases. Copyright 1998 John Wiley _ Sons, Inc.

Optimized enzymatic synthesis of XXXX in organic medium. Operating conditions and impact of different factors on kinetics

@entity13266 :: ('MESH:D019817', 'Chemical') :: ['benzoic acid', 'Benzoic acid'] @entity4315 :: ('CHEBI:29021', 'Chemical') :: ['hexane'] @entity1042 :: ('MESH:D000432', 'Chemical') :: ['methanol'] @entity8336 :: ('MESH:D014050', 'Chemical') :: ['toluene'] @entity69052 :: ('MESH:C044605', 'Chemical') :: ['methyl benzoate']
@entity69052 :: (MESH:C044605,Chemical) :: ['methyl benzoate']
