AIM: Existing evidence suggests that endothelial lipase ( @entity68617 ) plays an important role in high-densitylipoprotein (HDL) metabolism. Because @entity95 are a useful animal model for the study of @entity1 lipid metabolism and @entity731 , we characterized @entity95 @entity68617 ( @entity68617 ) expression and investigated its relationship with plasma HDL levels in normal and hyperlipidemic @entity95 . METHODS: We cloned the @entity68617 cDNA and analyzed the @entity68617 tissue expression using Northern blotting, real-time RT-PCR, Western blotting, and in situ hybridization. We evaluated the effects of @entity68617 antisense on plasma HDL levels. RESULTS: We found that @entity68617 mRNA was highly expressed in @entity165 synthesis-related organs, including the liver, testis, and adrenal along with its expression in the lung, kidney, bone marrow, and small intestine. Interestingly, @entity8577 ( @entity8577 ) @entity95 , a model of @entity1 @entity753 , had lower plasma levels of HDLs than normal @entity95 . The plasma HDL levels in @entity8577 @entity95 were inversely associated with high levels of plasma @entity68617 proteins and hepatic expression of @entity68617 mRNA. Injection of @entity68617 -specific antisense oligonucleotides into @entity95 resulted in the elevation of plasma large HDLs. Furthermore, we demonstrated that @entity68617 mRNA was expressed by both endothelial cells and macrophages in the lesions of @entity731 of @entity8577 @entity95 . CONCLUSIONS: @entity68617 is expressed in multiple tissues and may have many physiological and pathophysiological functions, such as in the regulation of @entity165 metabolism and @entity731 . Our results suggest that @entity68617 is an important regulator of plasma HDL levels in @entity95 .

Endothelial lipase mediates HDL levels in normal and hyperlipidemic XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity753 :: ('OMIM:143890', 'Disease') :: ['familial hypercholesterolemia'] @entity95 :: ('9986', 'Species') :: ['rabbits', 'rabbit'] @entity8577 :: ('MESH:D065627', 'Disease') :: ['Watanabe heritable hyperlipidemic', 'WHHL'] @entity68617 :: ('305584(Tax:10116)', 'Gene') :: ['EL', 'rEL'] @entity731 :: ('MESH:D050197', 'Disease') :: ['atherosclerosis', 'aortic atherosclerosis'] @entity165 :: ('MESH:D002784', 'Chemical') :: ['cholesterol']
@entity95 :: (9986,Species) :: ['rabbits']
