Polycomb group (PcG) protein-dependent histone methylation and ubiquitination drives chromatin compaction leading to reduced @entity5 suppressor expression and increased @entity5 cell survival. Green tea @entity6398 and @entity13710 ( @entity4970 ) hydrolase inhibitors are important candidate chemopreventive agents. Previous studies indicate that @entity3089 ( @entity3090 ), a potent green tea @entity3978 , suppresses PcG protein level and @entity1489 cell survival. Inhibition of AdoHcy hydrolase with @entity55584 (DZNep) inhibits methyltransferases by reducing @entity5498 group availability. In the present study, we examine the impact of @entity3090 and DZNep cotreatment on @entity1489 cell function. @entity3090 and DZNep, independently and in combination, reduce the level of PcG proteins including @entity16719 , @entity16718 , @entity38612 , @entity3751 and @entity3752 . This is associated with reduced H3K27me3 and H2AK119ub formation, histone modifications associated with closed chromatin. @entity17029 level is also reduced and acetylated H3 formation is increased. These changes are associated with increased @entity5 suppressor expression and reduced cell survival and are partially reversed by vector-mediated maintenance of @entity3752 level. The reduction in PcG protein level is associated with increased ubiquitination and is reversed by proteasome inhibitors, suggesting proteasome-associated degradation.

XXXX and DZNep reduce polycomb protein level via a proteasome-dependent mechanism in @entity1489 cells.

@entity3978 :: ('CHEBI:26195', 'Chemical') :: ['polyphenol'] @entity3751 :: ('7703', 'Gene') :: ['Mel18'] @entity16718 :: ('8726', 'Gene') :: ['eed'] @entity38612 :: ('23512', 'Gene') :: ['Suz12'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity4970 :: ('CHEBI:16680', 'Chemical') :: ['AdoHcy'] @entity1489 :: ('MESH:D012878', 'Disease') :: ['skin cancer'] @entity16719 :: ('2146', 'Gene') :: ['Ezh2'] @entity3752 :: ('648', 'Gene') :: ['Bmi-1'] @entity3089 :: ('MESH:C045651', 'Chemical') :: ['(-)-epigallocatechin-3-gallate'] @entity13710 :: ('MESH:D012435', 'Chemical') :: ['S-adenosylhomocysteine'] @entity3090 :: ('CHEBI:4806', 'Chemical') :: ['EGCG'] @entity55584 :: ('MESH:C048460', 'Chemical') :: ['3-deazaneplanocin A'] @entity5498 :: ('CHEBI:32875', 'Chemical') :: ['methyl'] @entity17029 :: ('3065', 'Gene') :: ['Histone deacetylase 1'] @entity6398 :: ('MESH:D059808', 'Chemical') :: ['polyphenols']
@entity3089 :: (MESH:C045651,Chemical) :: ['(-)-Epigallocatechin-3-gallate']
