STUDY DESIGN: @entity1 anulus cells were cultured under control and experimental conditions to study associations between proliferation and clinical-demographic features of subjects from which cells were obtained. Statistical multiple regression analyses were applied to develop mathematic models relating proliferation to age, gender, Thompson score (denoting stage of @entity1797 ), and status (control donor [postmortem]; surgical @entity1 ). OBJECTIVES: To identify the effect of donor characteristics on proliferative capacities of @entity1 disc cells. SUMMARY OF BACKGROUND DATA: As therapeutic options for @entity1797 increase, novel biologic options are important future considerations. Little is known about the influence of clinical-demographic features on cell proliferation. METHODS: Anulus cells were studied in two designs: 1) Cells from 12 individuals were grown in monolayer with 50 ng/mL interleukin growth factor-1 ( @entity442 ), 100 ng/mL insulin, or control conditions. 2) Cells from nine individuals were grown in three-dimensional culture with 10 ng/mL @entity442 or control conditions. Cell proliferation data and data on age, gender, Thompson score, and status were collected. Standard statistical analyses were used to develop correlation models. RESULTS: Data from monolayer experiments produced significant models fitting proliferation in the presence of low serum, 50 ng/mL @entity442 , or insulin, with age, gender, Thompson score, and status (respective R2: 0.827, 0.680, 0.850). Three-dimensional cultures exposed to 10 ng/mL @entity442 resulted in proliferation that correlated in a significant negative manner with Thompson score (r = -0.798). CONCLUSIONS: Clinical-demographic prognostic indicators may help predict levels of proliferation. Greater age, greater @entity1797 , female gender, and surgical derivation had deleterious effects on proliferation potential in this model.

Clinical and demographic prognostic indicators for XXXX disc cell proliferation in vitro: pilot study.

@entity1 :: ('9606', 'Species') :: ['human', 'patient', 'Human'] @entity1797 :: ('MESH:D055959', 'Disease') :: ['disc degeneration'] @entity442 :: ('3479', 'Gene') :: ['IGF-I']
@entity1 :: (9606,Species) :: ['human']
