BACKGROUND: @entity8674 is the most common @entity74 in Malaysia. Confirmation of the b-globin gene mutations involved in @entity2395 is usually carried out by molecular analysis of DNA extracted from leukocytes in whole blood. Molecular analysis is generally carried out when affected @entity1 are around 1 - 2 years as clinical symptoms are expressed during this period. Blood taking at this age can be distressing for the @entity1 . High yield and pure DNA extracted from non-invasive sampling methods can serve as alternative samples in molecular studies for @entity74 especially in pediatric cases. METHODS: In this study, mouthwash, saliva, and buccal cytobrush samples were collected from @entity2395 major @entity1 who had previously been characterized using DNA extracted from peripheral blood. DNA was extracted from mouthwash, saliva, and buccal cytobrush samples using the conventional inexpensive @entity3971 - @entity3636 method and was measured by spectrophotometry for yield and purity. Molecular characterization of b-globin gene mutations was carried out using the @entity262 ( @entity262 ). RESULTS: DNA extracted from mouthwash, saliva, and buccal cytobrush samples produced high concentration and pure DNA. The purified DNA was successfully amplified using @entity262 . Results of the b-globin gene mutations using DNA from the three non-invasive samples were in 100% concordance with results from DNA extracted from peripheral blood. CONCLUSIONS: The conventional in-house developed methods for non-invasive sample collection and DNA extraction from these samples are effective and negate the use of more expensive commercial kits. In conclusion, DNA extracted from mouthwash, saliva, and buccal cytobrush samples provided sufficiently high amounts of pure DNA suitable for molecular analysis of @entity2395 .

Non-Invasive DNA Sampling for Molecular Analysis of XXXX : Amiable Alternative Sampling Methods with Accurate Results for Pediatric @entity1 .

@entity1 :: ('9606', 'Species') :: ['children', 'patients', 'child'] @entity3636 :: ('MESH:D002725', 'Chemical') :: ['chloroform'] @entity262 :: ('MESH:D034721', 'Disease') :: ['ARMS', 'amplification refractory mutation system'] @entity3971 :: ('MESH:D019800', 'Chemical') :: ['phenol'] @entity74 :: ('MESH:D030342', 'Disease') :: ['genetic diseases', 'genetic disorder'] @entity8674 :: ('MESH:D017086', 'Disease') :: ['Beta-thalassemia'] @entity2395 :: ('MESH:D013789', 'Disease') :: ['b-thalassemia', 'thalassemia']
@entity8674 :: (MESH:D017086,Disease) :: ['Beta-Thalassemia']
