UNASSIGNED: Two-dimensional (2D) monolayer cell culture models are the most common method used to investigate @entity5 cells in vitro. In the few last decades, a multicellular spheroid model has gained attention due to its adjacency to @entity5 in vivo. The aim of the present study was to investigate immunohistochemical differences between these two cell culture systems. The FaDu, CAL27 and @entity1 @entity213 ( @entity213 ) cell lines were seeded out in monolayer and multicellular spheroids. The @entity1 and @entity1 cells were treated with increasing doses of @entity900 and irradiation. CAL27 cells were not used in theproliferation experiments, since the spheroids of CAL27 cells were not able to process the reagent in CCK-8 assays. Furthermore, they were stained to present alterations of the following antigens: Ki-67, vascular endothelial growth factor receptor, epithelial growth factor and survivin. Differences in growth rates and expression patterns were detected in certain @entity213 cell lines. The proliferation rates showed a significant divergence of cells grown in the three-dimensional model compared with cells grown in the 2D model. Overall, multicellular spheroids are a promising method to reproduce the immunohistochemical aspects and characteristics of @entity5 cells, and may show different response rates to therapeutic options.

Evaluation of spheroid XXXX cell models in comparison to monolayer cultures.

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity1 :: ('9606', 'Species') :: ['SCC25', 'FaDu'] @entity900 :: ('MESH:D002945', 'Chemical') :: ['cisplatin'] @entity213 :: ('MESH:C535575', 'Disease') :: ['head and neck squamous cell carcinoma', 'HNSCC']
@entity213 :: (MESH:C535575,Disease) :: ['head and neck squamous cell carcinoma']
