We have investigated the appearance and reorganization of tubulin-containing arrays induced by @entity3812 in the root meristem of @entity3505 @entity3505 , using immunostaining and electron microscopy. @entity3812 caused depolymerization of microtubules and formation of tubulin cortical strands composed of filamentous material only in C-mitotic cells. After prolonged exposure to the drug, both interphase and C-mitotic cells acquired needle-type bundles, arranged as different crystalloids and/or macrotubules. The unmodified tyrosinated form of @entity69169 was detected within microtubules in control cells, but was not found within cortical strands. It was identified, however, within needle-type bundles. The modified acetylated form of @entity69169 , which was absent in control cells, was detected within needle-type bundles. Thus, cortical strands were transitory arrays, transformed into needle-type bundles during prolonged exposure to @entity3812 . Cortical strands appeared in a cell cycle-dependent manner, whereas needle-type bundles were cell cycle stable arrays. The diverse morphological organization, intracellular distribution and stability of tubulin-containing arrays may be associated with heterogeneity of @entity69169 isoforms. We assume that non-microtubular arrays substitute for microtubules in conditions where normal tubulin polymerization is inhibited.

Time and cell cycle dependent formation of heterogeneous tubulin arrays induced by @entity3812 in XXXX root meristem.

@entity3505 :: ('4565', 'Species') :: ['Triticum aestivum', 'wheat'] @entity69169 :: ('543387(Tax:4565)', 'Gene') :: ['alpha-tubulin'] @entity3812 :: ('MESH:D003078', 'Chemical') :: ['colchicine', 'Colchicine']
@entity3505 :: (4565,Species) :: ['Triticum aestivum']
