OBJECTIVES: To study the resistance of biofilms developed by non-pigmented rapidly growing mycobacteria (NPRGM) against @entity4509 , @entity2177 and @entity4581 in an in vitro model using clinical strains of different species. DESIGN: Antimicrobial susceptibilities of different clinical strains of @entity7665 , @entity7665 chelonae, @entity7665 fortuitum, @entity7665 peregrinum, @entity7665 mucogenicum and @entity7665 mageritense using conventional techniques were measured. Biofilm resistance was measured by using the sandwich technique developed by Anderl et al. using a concentration of antibiotic of 50mg/L. Penetration of antibiotics through biofilm was measured using the same technique with minimal modifications. RESULTS: NPRGM biofilms showed drug resistance (percentages of viable bacteria >1% of those of controls) against antibiotics that are commonly used for the treatment of @entity281 caused by these organisms, although there are intraspecies differences between strains. We have detected differences in antibiotic penetration through biofilms with an important permeability barrier for @entity2177 . However, other mechanisms must be probably more important to explain the antimicrobial resistance of NPRGM biofilm. CONCLUSIONS: Biofilms formed by NPRGM are resistant to @entity4509 , @entity2177 and @entity4581 . As no resistance differences between the tested antibiotics have been observed, it is likely that biofilm permeability of antibiotics is of low importance for antimicrobial resistance of biofilms.

Importance of antibiotic penetration in the antimicrobial resistance of biofilm formed by non-pigmented rapidly growing mycobacteria against XXXX , @entity2177 and @entity4581 .

@entity4581 :: ('MESH:D017291', 'Chemical') :: ['clarithromycin'] @entity2177 :: ('MESH:D002939', 'Chemical') :: ['ciprofloxacin'] @entity4509 :: ('MESH:D000583', 'Chemical') :: ['amikacin'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infections'] @entity7665 :: ('36809', 'Species') :: ['Mycobacterium', 'Mycobacterium abscessus']
@entity4509 :: (MESH:D000583,Chemical) :: ['amikacin']
