Detection of @entity2737 is based on evaluation of the well-known signs of @entity32 like rubor (redness), calor (heat), @entity5 (swelling), and dolor ( @entity158 ) by medical doctors and/or time-consuming procedures requiring special machinery. There is currently no rapid diagnostic device available for the indication of @entity2737 , which would especially be helpful in home care of chronic @entity1066 @entity1 . In this study, a new concept for a fast diagnostic tool for @entity2737 based on @entity16977 and elastase triggered release of dye from a peptidoglycan matrix was investigated. The matrix consisted of alginate/agarose and peptidoglycan covalently labeled with Remazol brilliant blue. @entity16977 activity in @entity6081 and @entity6227 wound fluids was significantly elevated upon @entity281 (4830    1848 U mL(-1)) compared to noninfected wounds (376    240 U mL(-1)). Consequently, incubation of 8% (w/v) labeled agarose/peptidoglycan blend layers with @entity281 wound fluid samples for 2 h at 37   C resulted in a 4-fold higher amount of dye released than measured for noninfected wounds. For alginate/peptidoglycan beads, a 7-fold higher amount of dye was released in case of @entity281 wound fluid samples compared to noninfected ones. Apart from @entity16977 , proteases [i.e., gelatinase matrix metalloproteinase @entity1476 and @entity1495 and elastase] were detected in wound fluids (e.g., using Western blotting). When dosed in ratios typical for wounds, a slight synergistic effect was measured for peptidoglycan hydrolysis (i.e., dye release) between @entity16977 and these proteases. Incubation of a double-layer system consisting of stained and nonstained peptidoglycan with @entity281 wound fluids resulted in a color change from yellow to blue, thus allowing simple @entity227 .

Novel peptidoglycan-based diagnostic devices for detection of XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity227 :: ('MESH:D014786', 'Disease') :: ['visual detection of wound infection'] @entity16977 :: ('4069', 'Gene') :: ['lysozyme', 'Lysozyme'] @entity6081 :: ('MESH:D013530', 'Disease') :: ['postoperative wounds'] @entity1476 :: ('4313', 'Gene') :: ['MMP-2'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity1066 :: ('MESH:D014456', 'Disease') :: ['ulcer'] @entity2737 :: ('MESH:D014946', 'Disease') :: ['wound infection'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infected', 'infection'] @entity158 :: ('MESH:D010146', 'Disease') :: ['pain'] @entity1495 :: ('4318', 'Gene') :: ['MMP-9'] @entity6227 :: ('MESH:D003668', 'Disease') :: ['decubitus']
@entity2737 :: (MESH:D014946,Disease) :: ['wound infection']
