PURPOSE: Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) play important roles in the @entity32 in @entity66 models. However, their role in vein graft (VG) remodeling is undefined. We aimed to investigate the effect of EVs from @entity1958 MSCs (ADMSC-EVs) on VG intimal @entity1373 and to explore the possible mechanisms. METHODS: After generation and characterization of control-EVs and ADMSC-EVs in vitro, we investigated their effect on the proliferation and migration of vascular smooth muscle cells (VSMCs) in vitro. Next, we established a @entity19 model of VG transplantation. @entity19 underwent surgery and received control-EVs or ADMSC-EVs by intraperitoneal injection every other day for 20  days. VG remodeling was evaluated after 4  weeks. We also assessed the effect of ADMSC-EVs on macrophage migration and inflammatory cytokine expression. RESULTS: Significant inhibitory effects of ADMSC-EVs on in vitro VSMC proliferation (p   <   0.05) and migration (p   <   0.05) were observed compared with control-EVs. The extent of intimal @entity1373 was significantly decreased in ADMSC-EV-treated @entity19 compared with control-EV-treated @entity19 (26        8.4 vs. 45        9.0   m, p   <   0.05). A reduced presence of macrophages was observed in ADMSC-EV-treated @entity19 (p   <   0.05). Significantly decreased expression of inflammatory cytokines @entity777 and @entity1750 ( @entity1750 ) was also found in the ADMSC-EV-treated group (both p   <   0.05). In addition, phosphorylation of @entity1994 , @entity8837 , and @entity1995 in VGs was decreased in the ADMSC-EV-treated group. CONCLUSIONS: We demonstrated that ADMSC-EVs exert an inhibitory effect on VG @entity8233 formation by regulating VSMC proliferation and migration, macrophage migration, inflammatory cytokine expression, and the related signaling pathways.

Extracellular Vesicles Derived from XXXX Mesenchymal Stem Cells Regulate the Phenotype of Smooth Muscle Cells to Limit Intimal @entity1373 .

@entity32 :: ('MESH:D007249', 'Disease') :: ['reduction of inflammation'] @entity66 :: ('MESH:D004194', 'Disease') :: ['multiple disease'] @entity1750 :: ('20296(Tax:10090)', 'Gene') :: ['monocyte chemoattractant protein-1', 'MCP-1'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'Mice'] @entity1373 :: ('MESH:D006965', 'Disease') :: ['hyperplasia'] @entity1958 :: ('230796(Tax:10090)', 'Gene') :: ['adipose'] @entity777 :: ('16193(Tax:10090)', 'Gene') :: ['interleukin (IL)-6'] @entity1994 :: ('11651(Tax:10090)', 'Gene') :: ['Akt'] @entity1995 :: ('26416(Tax:10090)', 'Gene') :: ['p38'] @entity8837 :: ('26417;26413', 'Gene') :: ['Erk1/2'] @entity8233 :: ('MESH:D058426', 'Disease') :: ['neointima']
@entity1958 :: (230796(Tax:10090),Gene) :: ['Adipose']
