Probiotics based on Bacillus strains have been increasingly proposed for prophylactic and therapeutic use against several gastro-intestinal diseases. We studied safety for two Bacillus strains included in a popular East European probiotic. @entity3881 that was sensitive to all antibiotics listed by the European Food Safety Authority. @entity10948 was resistant to @entity2755 and @entity3402 . Both were non-hemolytic and did not produce Hbl or Nhe enterotoxins. No bceT and cytK toxin genes were found. Study of acute @entity137 in BALB/c @entity19 demonstrated no treatment-related @entity204 . The oral LD(50) for both strains was more than 2 x 10(11) CFU. @entity137 studies were performed on @entity19 , @entity95 , and @entity1707 and showed no signs of @entity137 or histological changes in either organs or tissues. We demonstrated that while certain risks may exist for the B. licheniformis strain considering antibiotic resistance, @entity3881 may be considered as non-pathogenic and safe for @entity1 consumption.

The safety of two Bacillus probiotic strains for XXXX use.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1707 :: ('9823', 'Species') :: ['pigs'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity', 'Chronic toxicity'] @entity10948 :: ('1402', 'Species') :: ['Bacillus licheniformis strain'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity95 :: ('9986', 'Species') :: ['rabbits'] @entity2755 :: ('MESH:D002701', 'Chemical') :: ['chloramphenicol'] @entity3881 :: ('1423', 'Species') :: ['Bacillus subtilis strain', 'B. subtilis strain'] @entity204 :: ('MESH:D003643', 'Disease') :: ['deaths'] @entity3402 :: ('MESH:D002981', 'Chemical') :: ['clindamycin']
@entity1 :: (9606,Species) :: ['human']
