BACKGROUND: Immunophenotyping has replaced cytochemical staining as the preferred technique for classifying @entity3052 . However, some @entity3052 ( @entity3052 ) lack lineage-associated markers. In our experience, @entity22252 ( @entity22252 ) is expressed in immature @entity235 monocytes. We hypothesized that @entity22252 is a useful marker for monocytic @entity3052 . OBJECTIVES: The objective was to compare @entity22252 expression in neoplastic cells from @entity235 with @entity706 , @entity1072 ( @entity1072 ), @entity981 ( @entity1619 ), and @entity3052 . METHODS: @entity22252 results were retrieved from medical records of @entity235 with @entity3052 . Smears from @entity235 with @entity706 or @entity1778 were also prospectively stained for @entity22252 activity. @entity1072 was based on persistent @entity6113 (10  *  10(9) /L) and @entity3052 on >=   20% blasts in blood or bone marrow. @entity1619 was classified based on positive phenotyping for T- or B-lymphocyte antigens, and @entity3052 on positive phenotyping for CD11b, CD11c or @entity31622 , or cytochemical staining for @entity2863 esterase, Sudan Black B, or myeloperoxidase. RESULTS: There was no @entity22252 activity in all 49 @entity706 and 7 CLLs. Weak @entity22252 activity was seen in 31% of 14 @entity1619 (all @entity1619 ). @entity22252 activity was seen in all 20 @entity3052 (P  <  .001 vs @entity1619 ) with strong activity in 64% (vs 25% @entity1619 ) in most neoplastic cells (median 75% vs 9% @entity1619 , P  =  .020). Of @entity3052 , 80% were @entity16129 + (vs 39% @entity1619 , P  =  .027) and 100% were MHCII- (vs 43% @entity1619 , P  =  .002). CONCLUSIONS: @entity22252 activity may be useful for @entity3052 confirmation in @entity235 , particularly if neoplastic cells only express @entity16129 + on immunophenotyping.

@entity22252 is a useful cytochemical marker for the diagnosis of acute myelomonocytic and XXXX in the @entity235 .

@entity6113 :: ('MESH:D008218', 'Disease') :: ['lymphocytosis'] @entity981 :: ('MESH:D054198', 'Disease') :: ['acute lymphoid leukemia'] @entity1619 :: ('MESH:D015458', 'Disease') :: ['T-ALL', 'ALL'] @entity706 :: ('MESH:D008223', 'Disease') :: ['lymphoma', 'lymphomas'] @entity235 :: ('9615', 'Species') :: ['canine', 'dogs'] @entity1072 :: ('MESH:D015451', 'Disease') :: ['CLL', 'chronic lymphocytic leukemia'] @entity31622 :: ('607076(Tax:9615)', 'Gene') :: ['CD14'] @entity3052 :: ('MESH:D015470', 'Disease') :: ['acute myeloid leukemias', 'AML', 'acute leukemia'] @entity2863 :: ('MESH:C006972', 'Chemical') :: ['chloroacetate'] @entity1778 :: ('MESH:D007938', 'Disease') :: ['leukemia'] @entity16129 :: ('415130(Tax:9615)', 'Gene') :: ['CD34'] @entity22252 :: ('403548(Tax:9615)', 'Gene') :: ['Alkaline phosphatase', 'ALP', 'alkaline phosphatase']
@entity1778 :: (MESH:D007938,Disease) :: ['monocytic leukemia']
