In an earlier publication we had reported the preparation of a rabbit antiserum specific for @entity35 Clara cell secretory proteins. This rabbit anti-rat Clara cell serum was found to react with two proteins in @entity35 lung lavage by crossed-immunoelectrophoresis. Immunoblotting of @entity35 lung lavage proteins, after @entity214 ( @entity214 ) @entity2975 gel electrophoresis, disclosed three bands of reactivity with anti-Clara cell serum. The relative molecular masses of these three proteins were about 200 (protein A) 55 (protein B) and about 12 kDa ( @entity38012 ). Anti-Clara cell antibodies eluted from Sepharose-4B-linked @entity38012 (as well as the antiserum raised by immunizing @entity95 with @entity38012 ) reacted with @entity38012 . Anti-Clara cell antiserum unbound to @entity38012 (as well as antiserum raised by immunizing @entity95 with protein B) reacted with protein B only. In non- @entity214 polyacrylamide gel electrophoresis, protein B migrated as a single band, slightly cathodic to albumin; @entity38012 resolved into three bands, all anodic to albumin. Immunoblots of isoelectric focusing gels showed three bands (pI 5.2-5.7) that reacted with antibody to @entity38012 , and four bands corresponding to protein B were seen in the pI range 4.6-5.0. As determined by immunoperoxidase staining of @entity1604 fixed methacrylate embedded 1 micron thick sections of @entity35 lung, protein(s) A (and @entity38012 ) and protein B were present in the same cells and in the same granules. Protein B was resistant to trypsin digestion, whereas @entity38012 were readily degraded by trypsin. @entity35 Clara cell secretory proteins consist of at least two antigenic types that appear to be functionally distinct, and each antigenic type displays charge microheterogeneity.

Antigenic, molecular and functional heterogeneity of Clara cell secretory proteins in the XXXX .

@entity95 :: ('9986', 'Species') :: ['rabbits'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity38012 :: ('25268(Tax:10116)', 'Gene') :: ['proteins A and C', 'protein C'] @entity35 :: ('10116', 'Species') :: ['rat', 'Rat'] @entity1604 :: ('MESH:C003043', 'Chemical') :: ['paraformaldehyde'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS', 'sodium dodecylsulphate']
@entity35 :: (10116,Species) :: ['rat']
