The aim was to characterize the variation in the cellular in vitro radiosensitivities in @entity957 of the head and neck, and to test for a possible correlation between different measures of radiosensitivity and the clinical and histopathological data. Cellular in vitro radiosensitivities were assessed in @entity5 biopsies from 71 @entity1 using the modified Courtenay-Mills soft agar clonogenic assay combined with an immunocytochemical analysis. Radiosensitivity was quantified as the surviving fraction after a radiation dose of 2 Gy irrespective of cell type (overall @entity19593 ), or based on identification of cell type ( @entity5 cell @entity19593 , fibroblast @entity19593 ). Sixty-three biopsies were from primary @entity5 , and eight were from recurrences. Overall plating efficiency ranged from 0.005 to 1.60% with a median of 0.052%. The majority of the colonies obtained from the biopsies were fibroblast marker-positive; the proportion of @entity5 marker-positive colonies ranged from 1 to 88% with a median of 15%. The median overall @entity19593 was 0.47 (range 0.24-0.96), the median @entity5 cell @entity19593 was 0.50 (range 0.11-1.0) and the median fibroblast @entity19593 was 0.49 (range 0.24-1.0). Comparing data from independent experiments, the overall @entity19593 was significantly correlated with the @entity19593 of fibroblasts (2P = 0.006) but not with the @entity5 cell @entity19593 . The @entity5 cell and fibroblast radiosensitivities measured in the same individuals were not correlated (r= 0.06, 95% CI [-0.19, 0.30]):This finding seems to preclude a strong correlation between the radiosensitivity of @entity5 cells and fibroblasts. Concerning the clinical characteristics, neither of the measures of @entity5 radiosensitivity was correlated with T- and N-category, stage, @entity5 size, sex and age. However, the @entity5 cell radiosensitivity decreased with increasing grade of histopathological differentiation (2P = 0.012). The same tendency was found in two independent analyses of the same @entity1 material. This correlation was not significant in case of the overall @entity19593 or the fibroblast @entity19593 .

In vitro radiosensitivity of XXXX cells and fibroblasts derived from head and neck carcinomas: mutual relationship and correlation with clinical data.

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumours', 'tumour'] @entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity957 :: ('MESH:D002294', 'Disease') :: ['squamous cell carcinomas'] @entity19593 :: ('6426', 'Gene') :: ['SF2']
@entity5 :: (MESH:D009369,Disease) :: ['tumour']
