INTRODUCTION: @entity1274 seriously influences a @entity1 's daily life, and no effective treatments exist to date. Gene therapy is a potential treatment for regenerating hair cells to restore hearing. METHODS: In this study, we established a @entity18129 model to study hair cell regeneration by co-administration of @entity7913 and @entity1483 . After the injections, we assessed the survival of outer hair cells (OHC), inner hair cells (IHC), supporting cells (SC), @entity528 ( @entity528 ) and peripheral axons. Moreover, we used two viral vectors to detect the transgene distribution. RESULTS: Our results showed at 12h post-treatment, numerous OHC were missing in the basal turn. At 24h post-treatment, all OHCs in basal half of the cochlea were lost, and by 48h, OHC loss had spread to the apical coil. Four days after the injections, all OHCs were absent. At 1mo post-treatment, the organ of Corti had collapsed. In contrast, most of the SC remained 4d after the injections. The loss of @entity528 and peripheral axons was consistent with this time course post-treatment. The results of transgene distribution suggested the correlative gene can be transferred into the organ of Corti using adenoviruses (AdV) vectors and lentiviruses (LV) vectors in our @entity18129 model. Comparison with Existing Method(s): We assessed the details of @entity204 at more time point and chosen the time point for gene transfer in this model. CONCLUSIONS: We conclude that this @entity18129 model would be suitable for the study of hair cell regeneration.

Establishment of a model of XXXX in rats to study potential gene therapy for @entity1274 .

@entity1 :: ('9606', 'Species') :: ['patient'] @entity7913 :: ('MESH:D007612', 'Chemical') :: ['kanamycin'] @entity1483 :: ('MESH:D005665', 'Chemical') :: ['furosemide'] @entity1274 :: ('MESH:D006319', 'Disease') :: ['Sensorineural hearing loss'] @entity528 :: ('MESH:D009410', 'Disease') :: ['spiral ganglion neurons', 'SGN'] @entity204 :: ('MESH:D003643', 'Disease') :: ['HC death'] @entity18129 :: ('MESH:D015834', 'Disease') :: ['cochlear lesions', 'cochlear lesion']
@entity18129 :: (MESH:D015834,Disease) :: ['cochlear lesions']
