UNASSIGNED: 184 Background: @entity263 shows a wide variation in the clinical incidence and mortality rates of different geographical regions. While it is the most common @entity2675 in Western countries, it is much less frequent in Asian countries. We investigated genomic changes in @entity263 from UK and China using microarrays to determine the genetic similarities and differences as well as the underlying mechanisms. METHODS: We determined genome-wide genomic alterations using Affymetrix SNP array 6.0, and evaluated data using fluorescence in situ hybridisation (FISH) and immunohistochemistry. In addition, we assess @entity342 induced @entity5435 and @entity9493 co-localization and fusion. Microsatellite analysis was used for AR CAG repeat polymorphism in UK and Chinese population. RESULTS: Genome-wide analysis of 32 UK and 39 Chinese samples revealed that losses of 21q22 (leading to @entity5435 : @entity9493 fusion) and 10q23.3 ( @entity983 ) were at much higher frequency in @entity263 . Using FISH analysis of 160 UK and 143 Chinese samples, we showed that @entity983 deletion and @entity9493 rearrangements were at a significantly higher frequency in samples from UK than China (p<0.001 for both). We found that @entity983 and @entity9493 protein were also differentially expressed (p<0.001) in the two populations. Investigating this further, we induced @entity5435 and @entity9493 gene proximity and @entity5435 : @entity9493 fusion in two immortalised prostate epithelial cell lines by exposure to high dose of @entity342 . This @entity342 treatment did not cause increased global DNA damage but was associated with low expression of @entity15660 , which is involved in repairing double-strand breaks. Overexpression of @entity15660 by transfection inhibited @entity342 induced @entity5435 : @entity9493 fusion. We found that AR CAG repeat lengths, which associated with AR activity, are significantly shorter in the UK than Chinese @entity1 (p<0.05). CONCLUSIONS: We revealed genomic differences in @entity263 comparing the high-risk (Western) and low-risk (Chinese) populations. We further demonstrated that @entity5435 : @entity9493 fusion can be induced by @entity342 . The difference of CAG repeat length between the two populations are potentially associated with @entity5435 : @entity9493 fusion positive @entity263 .

The different genetic alterations between Western and XXXX and the underlying mechanisms.

@entity5435 :: ('7113', 'Gene') :: ['TMPRSS2'] @entity1 :: ('9606', 'Species') :: ['patients'] @entity263 :: ('MESH:D011471', 'Disease') :: ['prostate cancers', 'prostate cancer', 'Prostate cancer', 'Western than Chinese prostate cancers'] @entity2675 :: ('MESH:D018567', 'Disease') :: ['male cancer'] @entity9493 :: ('2078', 'Gene') :: ['ERG'] @entity983 :: ('5728', 'Gene') :: ['PTEN'] @entity342 :: ('CHEBI:50113', 'Chemical') :: ['androgen'] @entity15660 :: ('9271', 'Gene') :: ['PIWIL1']
@entity263 :: (MESH:D011471,Disease) :: ['Chinese prostate cancers']
