In @entity4328 , the cadA gene encodes the cell adhesion molecule DdCAD-1, a protein of M(r) 24,000, which mediates @entity141 -dependent cell-cell adhesion during development. We have examined the effects of @entity268 , cell-cell contact, and growth conditions on cadA expression. cadA has a unique pattern of expression, which appears to be a combination of the expression patterns of early genes and aggregation-stage genes. Expression of the cadA gene in bacterially grown cells is activated at the beginning of the developmental cycle, followed by a period of rapid DdCAD-1 accumulation. The mRNA level reaches its maximum at 9 h of development and then declines to the basal level at approximately 18 h, while the protein level remains constant after reaching its maximum at 12 h. Pulse-chase experiments have demonstrated that DdCAD-1 has a significantly longer half-life than the average cellular protein. Transcription of the cadA gene is stimulated by exogenous @entity268 pulses, leading to a 3- to 5-fold increase in the transcription rate. In the fgdA mutant, which lacks a functional G alpha 2, @entity268 fails to enhance cadA expression, suggesting that @entity268 stimulates cadA transcription via a G protein-dependent pathway. However, inhibition of cell-cell contact has no effect on the synthesis of DdCAD-1. Growth conditions also have a major influence on cadA expression. Axenically grown cells produce a high level of cadA transcripts during vegetative growth. The mRNA level shows a steady decrease during development and is reduced to the basal level by 12 h. In contrast, the level of DdCAD-1 remains relatively high throughout development, suggesting that axenic growth affects the accumulation of cadA mRNA but not the stability of the protein. These results indicate that multiple mechanisms are involved to maintain a high level of DdCAD-1 during development.

Synthesis of the XXXX -dependent cell adhesion molecule DdCAD-1 is regulated by multiple factors during @entity4328 development.

@entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity4328 :: ('44689', 'Species') :: ['Dictyostelium discoideum'] @entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP']
@entity141 :: (CHEBI:29108,Chemical) :: ['Ca(2+)']
