BACKGROUND: Susceptibility of lipoprotein to oxidation is usually studied using purified lipoproteins. However, for large clinical studies or routine clinical assessment, a rapid less time-consuming method is desirable. Therefore, we studied @entity920 -mediated oxidation of the macromolecule fraction of plasma in comparison with oxidation directly in whole unfractionated plasma in a group of hyperlipidemic individuals. METHODS: Lag phase rate (LR), propagation phase rate (PR), lag time (LT), and maximal extent of oxidation were determined for @entity920 -mediated oxidation in plasma from 16 hyperlipidemic individuals. Oxidation parameters obtained for whole plasma (WP) and macromolecules were subjected to correlation analysis with plasma lipid concentrations and with the @entity3952 / @entity4722 ratio (18:1/18:2) in phospholipids (PL), @entity1674 (TG), and @entity8557 (CE). RESULTS: Total @entity165 (TC) concentration was significantly correlated with lag rate (negative, p<0.05), lag time (positive, p<0.01), and with maximal extent of oxidation (positive, p<0.05) for plasma macromolecules (PM). @entity412 concentration was not significantly correlated with lag rate, lag time, or propagation rate for plasma macromolecules. @entity412 concentration was positively correlated with the maximal extent of oxidation (p<0.01) for plasma macromolecules. In the @entity14839 fraction, the 18:1/18:2 ratio was significantly correlated (positive, p<0.05) with lag time. Phospholipid 18:1/18:2 ratio did not correlate with oxidative parameters. This ratio in @entity1674 correlated better, but statistical significance was not obtained. Parameters obtained with whole plasma did not significantly correlate with lipid values. CONCLUSIONS: Oxidation parameters obtained for plasma macromolecules correlated with lipid parameters known to be associated with oxidative susceptibility of lipoproteins and were unaffected by soluble antioxidants found in whole plasma or serum. Therefore, plasma macromolecules were superior to whole plasma for assessing lipoprotein susceptibility to oxidation. This approach will facilitate larger clinical trials by saving the time and labor involved in lipoprotein isolation.

Lipoprotein susceptibility to oxidation in the macromolecular fraction of plasma: relation to plasma lipids and oleate/ XXXX ratio in comparison with whole plasma in hypertriglyceridemia.

@entity14839 :: ('CHEBI:17002', 'Chemical') :: ['cholesterol ester'] @entity3952 :: ('MESH:D019301', 'Chemical') :: ['oleic acid'] @entity1674 :: ('MESH:D014280', 'Chemical') :: ['triglycerides'] @entity412 :: ('CHEBI:17855', 'Chemical') :: ['Triglyceride'] @entity920 :: ('MESH:D003300', 'Chemical') :: ['copper'] @entity8557 :: ('MESH:D002788', 'Chemical') :: ['cholesterol esters'] @entity4722 :: ('MESH:D019787', 'Chemical') :: ['linoleic acid'] @entity165 :: ('MESH:D002784', 'Chemical') :: ['cholesterol']
@entity4722 :: (MESH:D019787,Chemical) :: ['linoleate']
