UNASSIGNED: Economically important plant species, such as Eucalyptus globulus, are often rooting recalcitrant. We have previously shown that far-red light enrichment applied to E.  globulus donor-plants improved microcutting rooting competence and increased rooting zone/shoot @entity1735 ratio. To better understand this developmental response, the relative expression profiles of genes involved in auxin signaling ( @entity16258 , ARF8, @entity21178 ), biosynthesis (YUC3) and transport (AUX1, @entity41543 , @entity7071 ); @entity969 cleavage (SUS1, CWINV1), transport (SUC5), @entity12015 phosphorylation (HXK1, @entity18493 ) and starch biosynthesis (SS3) were quantified during adventitious rooting of E.  globulus microcuttings derived from donor plants exposed to far-red or white light. Expression of auxin transport-related genes increased in the first days of root induction. Far-red enrichment of donor plants induced @entity16258 , ARF8 and @entity21178 in microcuttings. The first two gene products could activate GH3 and other rooting related genes, whereas @entity21178 deregulation of the repressor ARF17 may relief rooting inhibition. Increased sink strength at the basal stem with @entity969 unloading in root tissue mediated by SUC and subsequent hydrolysis by SUS1 were also supported by gene expression profile. @entity948 phosphorylation and starch biosynthesis could also contribute to proper @entity505 allocation at the site of rooting, as evidenced by increased expression of related genes. These data are in good agreement with increased contents of hexoses and starch at the cutting base severed from far-red exposed donor plants. To sum up, pathways integrating auxin and @entity1735 metabolism were activated in microcuttings derived from donor plants exposed to far red light enrichment, thereby improving rooting response in E.  globulus.

Concerted transcription of auxin and XXXX homeostasis-related genes underlies improved adventitious rooting of microcuttings derived from far-red treated Eucalyptus globulus Labill mother plants.

@entity948 :: ('MESH:D005632', 'Chemical') :: ['Fructose'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['carbohydrate'] @entity12015 :: ('CHEBI:18133', 'Chemical') :: ['hexose'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity16258 :: ('382', 'Gene') :: ['ARF6'] @entity41543 :: ('5300', 'Gene') :: ['PIN1'] @entity18493 :: ('2316', 'Gene') :: ['FLN1'] @entity505 :: ('MESH:D002244', 'Chemical') :: ['carbon'] @entity21178 :: ('26523', 'Gene') :: ['AGO1'] @entity7071 :: ('7013', 'Gene') :: ['PIN2']
@entity1735 :: (CHEBI:16646,Chemical) :: ['carbohydrate']
