The KS 300 is a multifunctional software image analysis system using an object-oriented programming environment. The possibility of its application for the inner ear was studied by using specimens from @entity1 and squirrel monkeys, immunostained for the brain-derived @entity460 -binding protein, @entity4483 protein. Grey images were used for measurements. The cell borders were outlined by hand, using a digitizer. The absolute grey values of the pixels changed when the brightness of the images or other conditions changed. By contrast, the relative grey values, i.e. the absolute grey values correlated to the mean grey values of the histoimage, remained constant. By utilizing these relative grey values, it was possible to compare cells both between different specimens and between different areas within the same specimen. The different grey values of @entity4494 cells stained for @entity4483 protein are objective quantitative measurements and are believed to reflect differences in their function. In some regions of both @entity1 and squirrel monkey specimens, relatively intensely stained cells predominated, whereas in other regions, relatively weakly stained cells were mainly observed. Thus, our image analysis system using the relative grey values has proved suitable for quantitative analysis of immunostained specimens in order to compare them and to assess cell function.

Image analysis of the XXXX inner ear.

@entity1 :: ('9606', 'Species') :: ['humans', 'human'] @entity4494 :: ('MESH:D045888', 'Disease') :: ['spiral ganglion'] @entity4483 :: ('6285', 'Gene') :: ['S-100'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium']
@entity1 :: (9606,Species) :: ['human']
