The @entity630 -associated chemokines @entity12426 , @entity15369 , and @entity25414 coordinate migration of @entity14013 (+) @entity630 cells. The objective of this study was to evaluate the role of the innate immune system in stimulating chemokine expression in an experimental model of @entity936 and bridge the gap between innate and adaptive immunity. Desiccating stress (DS) induced very early (6 h) expression and production of @entity630 -associated chemokines in @entity267 and conjunctiva of C57BL/6 and @entity5350 knockout (KO) @entity19 , demonstrating that chemokine expression does not require innate T cells. We then demonstrated that activating the innate immune system prior to adoptive transfer of T cells to RAG1KO @entity19 increased disease severity. Interestingly, lack of induction of chemokines @entity12426 , @entity15369 , and @entity25414 in IFN-yKO @entity19 provided evidence that their expression requires IFN-y for induction. Treatment of @entity5350 KO mice with anti- @entity11386 prevented the increase of @entity12426 , @entity15369 , and @entity25414 in response to DS, compared with isotype controls. Additionally, DS increased the expression of @entity3132 in the conjunctiva. The expression of the @entity3132 ligand, @entity1968 early inducible gene 1, also increased at the ocular surface at both the protein and gene levels. Neutralization of @entity3132 at the ocular surface decreased the expression of @entity12426 , @entity15369 , @entity25414 , and IFN-y. In summary, upregulation of @entity12426 , @entity15369 , and @entity25414 expression in experimental @entity936 is T cell-independent, requiring IFN-y-producing @entity3132 (+) NK cells that are activated in response to DS-induced stress signals. This study provides insight into the events that trigger the initial immune response in @entity936 pathology.

Desiccating Stress-Induced Chemokine Expression in the Epithelium Is Dependent on Upregulation of XXXX /RAE-1 and Release of IFN-y in Experimental Dry Eye.

@entity12426 :: ('17329(Tax:10090)', 'Gene') :: ['CXCL9'] @entity267 :: ('MESH:D065306', 'Disease') :: ['cornea'] @entity14013 :: ('12766(Tax:10090)', 'Gene') :: ['CXCR3'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity25414 :: ('56066(Tax:10090)', 'Gene') :: ['CXCL11'] @entity11386 :: ('17059(Tax:10090)', 'Gene') :: ['NK1.1'] @entity936 :: ('MESH:D015352', 'Disease') :: ['dry eye'] @entity5350 :: ('19373(Tax:10090)', 'Gene') :: ['RAG1'] @entity3132 :: ('27007(Tax:10090)', 'Gene') :: ['NKG2D'] @entity630 :: ('57314(Tax:10090)', 'Gene') :: ['Th1'] @entity1968 :: ('MESH:D014212', 'Chemical') :: ['retinoic acid'] @entity15369 :: ('15945(Tax:10090)', 'Gene') :: ['CXCL10']
@entity3132 :: (27007(Tax:10090),Gene) :: ['NKG2D']
