Intracellular pools of three @entity9931 molecular species of coenzyme A, CoASH, @entity6625 , and @entity1285 , in @entity2624 cells were studied by @entity8595 cycling method in replacement culture. The sizes and compositions of @entity9931 pools starved for a @entity505 source changed within minutes after the addition of one of various @entity505 sources. A large @entity6625 pool formed after the addition of @entity413 , @entity8691 , @entity3913 , @entity653 , or @entity5121 , but there was little change when @entity413 , @entity969 , @entity7969 , @entity4588 , or @entity598 was added. The beta-anomer of @entity413 was assimilated 10 times faster than the alpha-anomer. Intracellular @entity9931 pools also changed with stress: in the pH, incubation temperature, or with osmotic stress. The sizes and compositions of @entity9931 pools were not affected by pH changing between 4 and 8, but the breakdown of @entity6625 and CoASH was greater at pH 9 than at pH 4 to 8. Production of @entity6625 was greatest at 40 degrees C, and at 50 degrees C, an @entity6625 pool did not form at all and the size of the CoASH pool declined. When the organism was stressed by the addition of @entity1219 at concentrations of more than 0.6 M, little @entity6625 was produced. The total @entity9931 pool (the sum of the concentrations of CoASH, @entity6625 , and @entity1285 ) remained within the limits of 0.83-1.40 nmol/mg of dry cell weight (0.30-0.52 mM). Whenever @entity6625 increased, CoASH decreased. Therefore, the @entity6625 /CoASH ratio is an important index of facultative anaerobes that reflects the state of @entity505 and energy metabolism in vivo.

Changes in size of intracellular pools of coenzyme A and its thioesters in @entity2624 cells to various XXXX sources and stresses.

@entity8691 :: ('CHEBI:48095', 'Chemical') :: ['D-fructose'] @entity7969 :: ('MESH:D008320', 'Chemical') :: ['maltose'] @entity4588 :: ('MESH:D019802', 'Chemical') :: ['succinate'] @entity1285 :: ('MESH:D008316', 'Chemical') :: ['malonyl-CoA'] @entity5121 :: ('MESH:D013012', 'Chemical') :: ['sorbitol'] @entity1219 :: ('CHEBI:26710', 'Chemical') :: ['NaCl'] @entity598 :: ('CHEBI:47622', 'Chemical') :: ['acetate'] @entity653 :: ('MESH:D005990', 'Chemical') :: ['glycerol'] @entity9931 :: ('MESH:D003065', 'Chemical') :: ['CoA'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['L-glucose', 'D-glucose'] @entity3913 :: ('MESH:D008358', 'Chemical') :: ['D-mannose'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity8595 :: ('MESH:D000214', 'Chemical') :: ['acyl-CoA'] @entity505 :: ('MESH:D002244', 'Chemical') :: ['carbon'] @entity6625 :: ('MESH:D000105', 'Chemical') :: ['acetyl-CoA'] @entity2624 :: ('83333', 'Species') :: ['Escherichia coli K-12']
@entity505 :: (MESH:D002244,Chemical) :: ['carbon']
