The induction of @entity1495 is particularly important for the invasiveness of various @entity5 cells. @entity57037 , a natural @entity1524 derivative extracted from traditional Chinese medicines, is known to inhibit the proliferation of a variety of @entity5 cells, but the effect of osthole on the invasiveness of @entity5 cells is largely unknown. This study determines whether and by what mechanism osthole inhibits invasion in CL1-5 @entity1 @entity2220 cells. Herein, we found that osthole effectively inhibited the migratory and invasive abilities of CL1-5 cells. A zymographic assay showed that osthole inhibited the proteolytic activity of @entity1495 in CL1-5 cells. Inhibition of migration, invasion, and @entity1476 and/or @entity1495 proteolytic activities was also observed in other @entity2220 cell lines (H1299 and A549). We further found that osthole inhibited @entity1495 expression at the messenger RNA and protein levels. Moreover, a chromatin immunoprecipitation assay showed that osthole inhibited the transcriptional activity of @entity1495 by suppressing the DNA binding activity of nuclear factor (NF)-kB in the @entity1495 promoter. Using reporter assays with point-mutated promoter constructs further confirmed that the inhibitory effect of osthole requires an NF-kB binding site on the @entity1495 promoter. Western blot and immunofluorescence assays demonstrated that osthole inhibited NF-kB activity by inhibiting IkB-a degradation and NF-kB @entity9135 nuclear translocation. In conclusion, we demonstrated that osthole inhibits NF-kB-mediated @entity1495 expression, resulting in @entity2 cell invasion and migration, and osthole might be a potential agent for preventing the invasion and @entity3 of @entity2 .

Osthole inhibits the invasive ability of @entity1 XXXX cells via suppression of NF-kB-mediated matrix metalloproteinase-9 expression.

@entity1 :: ('9606', 'Species') :: ['human'] @entity9135 :: ('5970', 'Gene') :: ['p65'] @entity1476 :: ('4313', 'Gene') :: ['MMP2'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity1524 :: ('MESH:C030123', 'Chemical') :: ['coumarin'] @entity2 :: ('MESH:D008175', 'Disease') :: ['suppression of lung cancer', 'lung cancer'] @entity57037 :: ('MESH:C046627', 'Chemical') :: ['Osthole'] @entity1495 :: ('4318', 'Gene') :: ['MMP-9', 'matrix metalloproteinase (MMP)-9'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity2220 :: ('MESH:C538231', 'Disease') :: ['lung adenocarcinoma']
@entity2220 :: (MESH:C538231,Disease) :: ['lung adenocarcinoma']
