We developed a minimally invasive method for producing left ventricular (LV) pressure overload in @entity19 . With the use of this technique, we quickly and reproducibly banded the transverse aorta with low surgical morbidity and mortality. Minimally invasive transverse aortic banding (MTAB) acutely and chronically increased LV systolic pressure, increased heart weight-to-body weight ratio, and induced @entity296 . We used this technique to determine whether reduced insulin signaling in the heart altered the cardiac response to pressure overload. @entity19 with cardiac myocyte-restricted knockout of the @entity3239 have smaller hearts than @entity164 ( @entity164 ) controls. Four weeks after MTAB, @entity164 and @entity3239 @entity19 had comparably increased LV systolic pressure, increased cardiac mass, and induction of mRNA for beta-myosin heavy chain and @entity14662 . However, @entity3239 hearts were more dilated, had depressed LV systolic function by echocardiography, and had greater interstitial @entity82 than @entity164 @entity19 . Expression of connective tissue growth factor was increased in banded @entity3239 hearts compared with @entity164 hearts. Thus lack of insulin signaling in the heart accelerates the transition to a more decompensated state during cardiac pressure overload. The use of the MTAB approach should facilitate the study of the pathophysiology and treatment of pressure-overload @entity1184 .

Minimally invasive aortic banding in XXXX : effects of altered cardiomyocyte insulin signaling during pressure overload.

@entity14662 :: ('230899(Tax:10090)', 'Gene') :: ['atrial natriuretic factor'] @entity296 :: ('MESH:D009202', 'Disease') :: ['myocardial fibrosis'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis'] @entity19 :: ('10090', 'Species') :: ['mice', 'Mice'] @entity1184 :: ('MESH:D006984', 'Disease') :: ['hypertrophy'] @entity3239 :: ('16337(Tax:10090)', 'Gene') :: ['insulin receptor (CIRKO)', 'CIRKO'] @entity164 :: ('MESH:D017827', 'Disease') :: ['WT', 'wild-type']
@entity19 :: (10090,Species) :: ['mice']
