BACKGROUND: In vitro maturation (IVM) of immature oocytes collected from ovary has been proposed for fertility preservation. In addition, quality of oocytes post IVM is one of the factors determining its developmental competence. By using the non-invasive Polscope system, both @entity189 ( @entity189 ) and zona pellucida (ZP) can be assessed in living oocytes. OBJECTIVE: The aim was to investigate the developmental potential of immature oocytes retrieved from ovarian tissue after IVM, as a method for fertility preservation, in @entity1 with @entity13374 . MATERIALS AND METHODS: The ovarian cortex from 26 @entity1 with malignant and benign diseases (21-45 years old), were obtained directly from collaborating hospitals, and transported to the IVF center on ice. In total 61 immature oocytes were aspirated, of which 18 (29.5%) were degenerated and discarded. The remaining 43 (70.5%) healthy oocytes were cultured in IVM culture media for 48 hr. The rate of maturity was assessed, and the ZP birefringence and @entity189 were imaged with Polscope technology. RESULTS: Overall 43 immature oocytes underwent IVM technology, of which 30.2% reached viable metaphase II (MII) oocytes. The ovarian tissues of 9 (34.6%) @entity1 were lacking oocytes at any stage. During polarized light microscopy examination, @entity189 could be visualized only in one of the MII oocytes, but high ZP birefringence's were observed in the majority of the oocytes post IVM (61.5%). CONCLUSION: Oocytes maturation post IVM from unstimulated ovaries showed a good developmental competence in gynecologic @entity1 . Further studies should be performed to advance the oocyte maturation program, such as co-culture system, for fertility preservation.

Developmental competence of immature oocytes aspirated from antral follicles in XXXX with @entity13374 .

@entity1 :: ('9606', 'Species') :: ['women', 'patients'] @entity189 :: ('MESH:D002277', 'Disease') :: ['meiotic spindle', 'MS'] @entity13374 :: ('MESH:D005831', 'Disease') :: ['gynecological diseases']
@entity1 :: (9606,Species) :: ['patients']
