BACKGROUND: @entity1576 caused by @entity1800 is associated with the development and exacerbation of childhood @entity565 . Little is known about the effects of respiratory @entity1800 in the absence of illness. Using quantitative PCR (qPCR) for common respiratory viruses and for two genes known to be highly upregulated in @entity1800 ( @entity12869 / @entity372 ), we screened 92 asthmatic and 69 healthy @entity1 without illness for @entity1729 . RESULTS: We found 21 viral qPCR-positive and 2 suspected @entity778 subjects with high expression of @entity12869 / @entity372 . We applied a dual RNA-seq workflow to these subjects, together with 25 viral qPCR-negative subjects, to compare qPCR with sequencing-based virus detection and to generate the airway transcriptome for analysis. RNA-seq virus detection achieved 86% sensitivity when compared to qPCR-based screening. We detected additional respiratory viruses in the two @entity12869 / @entity372 -high subjects and in two of the qPCR-negative subjects. Viral read counts varied widely and were used to stratify subjects into Virus-High and Virus-Low groups. Examination of the host airway transcriptome found that the Virus-High group was characterized by immune cell airway infiltration, downregulation of cilia genes, and dampening of type 2 @entity32 . Even the Virus-Low group was differentiated from the No-Virus group by 100 genes, some involved in @entity38270 signaling. CONCLUSIONS: @entity1729 without illness is not innocuous but may determine the airway function of these subjects by driving immune cell airway infiltration, cellular remodeling, and alteration of asthmogenic gene expression.

Dual RNA-seq reveals @entity1800 in asthmatic @entity1 without XXXX which are associated with changes in the airway transcriptome.

@entity1 :: ('9606', 'Species') :: ['children'] @entity778 :: ('MESH:D015658', 'Disease') :: ['virus-infected'] @entity12869 :: ('6355', 'Gene') :: ['CCL8'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity565 :: ('MESH:D001249', 'Disease') :: ['asthma'] @entity1800 :: ('MESH:D014777', 'Disease') :: ['viral infections', 'viral infection'] @entity1576 :: ('MESH:D012131', 'Disease') :: ['Respiratory illness'] @entity1729 :: ('MESH:D012141', 'Disease') :: ['Respiratory virus infection', 'respiratory virus infections'] @entity38270 :: ('8894', 'Gene') :: ['eIF2'] @entity372 :: ('6373', 'Gene') :: ['CXCL11']
@entity1576 :: (MESH:D012131,Disease) :: ['respiratory illness']
