AIMS: @entity1875 ( @entity2848 ), an intravenous anesthetic agent, is previously reported to attenuate oxidative stress- and @entity32 -induced endothelial cell dysfunction. This study investigated its effect on endothelial cell biology. MAIN METHODS: Cultured @entity1 umbilical vein endothelial cells (HUVECs) were treated with @entity2848 and subject to measurements for @entity842 (NO) production, autophagy flux, signal transduction, migration, and in vitro angiogenesis. KEY FINDINGS: Non-cytotoxic @entity2848 treatment was found to significantly upregulate inducible @entity842 synthase ( @entity3623 ) but downregulate constitutive @entity9554 expression. It also potentiated @entity558 -induced @entity2071 overexpression and NO overproduction. Mechanistically, the @entity2848 -activated signal transduction in PI3K/ @entity584 , @entity1366 , p38 MAPK, and @entity4675 pathways were involved in the @entity2848 -driven NO overproduction. @entity2848 exhibited a stimulatory effect on autophagy flux by increasing expression of autophagy markers including @entity3470 , @entity8783 , @entity10614 , and LC3I/II, as well as a late endosomal indicator, @entity21219 . However, @entity2848 appeared to antagonize the @entity21219 upregulation by @entity558 . Functionally, @entity2848 enhanced in vitro migratory and angiogenic capacities of HUVECs, but this enhancement was drastically abrogated by the presence of autophagy inhibitors, indicating a pro-angiogenic contribution of @entity2848 -enhanced autophagy in cultured HUVECs. SIGNIFICANCE: Our findings support the notion that @entity2848 enhances motility and angiogenic capacity of cultured HUVECs through an autophagy-involved regulatory mechanism.

@entity1875 -enhanced autophagy increases motility and angiogenic capacity of cultured XXXX umbilical vascular endothelial cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity558 :: ('MESH:C536528', 'Disease') :: ['LPS'] @entity9554 :: ('4846', 'Gene') :: ['NOS3'] @entity21219 :: ('338382', 'Gene') :: ['Rab7'] @entity584 :: ('207', 'Gene') :: ['Akt'] @entity10614 :: ('9474', 'Gene') :: ['Atg5'] @entity3470 :: ('21977(Tax:10090)', 'Gene') :: ['mTOR'] @entity8783 :: ('8678', 'Gene') :: ['Beclin-1'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity1875 :: ('MESH:D015742', 'Chemical') :: ['Propofol'] @entity2071 :: ('3383', 'Gene') :: ['ICAM-1'] @entity4675 :: ('5599', 'Gene') :: ['JNK'] @entity3623 :: ('4843', 'Gene') :: ['NOS2'] @entity1366 :: ('5595;5594', 'Gene') :: ['ERK1/2'] @entity2848 :: ('CHEBI:50459', 'Chemical') :: ['PPF']
@entity1 :: (9606,Species) :: ['human']
