Increased accumulation of lipoproteins and @entity165 within cells from regenerated endothelium may be responsible for their reported dysfunction. This study compared the presence and uptake of oxidized forms of low-density lipoprotein (LDL) in cells derived from native and regenerated endothelium. Four weeks after balloon denudation, primary cultures of native and regenerated endothelial cells were prepared from porcine coronary arteries. Regenerated endothelium stained more strongly using an antibody against oxidized lipoproteins. The increase in oxidized forms of @entity69974 exhibited by cells from regenerated endothelium was not due to an increase in extracellular-induced oxidation of native LDL, measured as the production of @entity4466 -reactive substances, being identical in both cell types. Intracellular @entity165 and @entity14839 content were unchanged in regenerated cells. Using flow cytometry, accumulation of oxidized LDL was investigated further by quantifying the uptake of a mildly oxidized preparation of @entity9717 -labelled LDL. The parameters of uptake, EC(50) and E(max), were not different between cells from native and regenerated endothelium suggesting that the number of @entity69975 receptors was identical in the two cell types. Moreover, a negative correlation between the increased uptake of acetylated LDL and decreased @entity6708 production in response to @entity1812 was observed in cells from regenerated endothelium. Thus, the increased incorporation of modified LDL and their intracellular oxidation could be responsible for the alteration in NO production. The presence of oxidized forms of LDL may be a marker of endothelium regeneration and could be involved in the endothelial dysfunction of @entity1707 coronary arteries 4 weeks after balloon denudation.

Cells derived from regenerated endothelium of the porcine coronary artery contain more oxidized forms of XXXX without a modification in the uptake of oxidized LDL.

@entity6708 :: ('CHEBI:16356', 'Chemical') :: ['cGMP'] @entity1707 :: ('9823', 'Species') :: ['pig'] @entity14839 :: ('CHEBI:17002', 'Chemical') :: ['cholesterol ester'] @entity69974 :: ('100523371', 'Gene') :: ['apolipoprotein-B-100'] @entity4466 :: ('MESH:C029684', 'Chemical') :: ['thiobarbituric-acid'] @entity1812 :: ('MESH:D001920', 'Chemical') :: ['bradykinin'] @entity69975 :: ('396724(Tax:9823)', 'Gene') :: ['LOX-1'] @entity165 :: ('MESH:D002784', 'Chemical') :: ['cholesterol'] @entity9717 :: ('MESH:C024286', 'Chemical') :: ["1,1'-dioctadecyl-3,3,3',3-tetramethyl-indocarbocyanine perchlorate"]
@entity69974 :: (100523371,Gene) :: ['apolipoprotein-B-100']
