Kappa-opioid receptor (OR) stimulation with a selective agonist, U50,488H (U50), known to mediate the delayed cardioprotection of @entity209 ( @entity209 ) against @entity204 in @entity35 ventricular myocytes, has been shown to act via @entity7131 ( @entity7131 ). We attempted to identify the @entity7131 isoform(s) that is activated, thus triggering delayed cardioprotection of @entity209 and pretreatment with 10 microM U50 (U50 pretreatment, UP). Release of @entity880 dehydrogenase and exclusion of @entity3460 by isolated @entity35 ventricular myocytes were used as indices of cell injury and @entity204 , respectively. Both @entity209 and UP induced translocation of @entity7131 , but not other @entity7131 isoforms, -alpha and -delta, from cytosolic to membrane fractions. This was accompanied by reductions in @entity204 induced by lethal simulated @entity63 . The effects of @entity209 and UP were attenuated and abolished by 1 microM @entity14719 , a selective kappa-OR antagonist, administered before and during preconditioning/pretreatment, respectively. The effects were mimicked by 10 nM @entity1359 , a @entity7131 activator, but attenuated by 5 microM @entity668 , a @entity7131 inhibitor. More importantly, 0.1 microM epsilonV1-2, a selective @entity7131 inhibitor administered before and during @entity209 /UP, also attenuated the effects of both treatments on @entity204 and translocation of @entity7131 . On the other hand, 5 microM @entity12703 , a selective @entity7131 -delta inhibitor, did not alter the effects of either treatment on @entity204 . The results indicate that both @entity209 and UP activate @entity7131 , leading to delayed cardioprotection in @entity35 ventricular myocytes.

Protein kinase C-epsilon is a trigger of delayed cardioprotection against myocardial ischemia of kappa-opioid receptor stimulation in XXXX ventricular myocytes.

@entity35 :: ('10116', 'Species') :: ['rat'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemia'] @entity12703 :: ('MESH:C085746', 'Chemical') :: ['rottlerin'] @entity668 :: ('MESH:C016299', 'Chemical') :: ['chelerythrine'] @entity1359 :: ('CHEBI:37537', 'Chemical') :: ['phorbol-12-myristate-13-acetate'] @entity7131 :: ('MESH:D020151', 'Disease') :: ['protein kinase C', 'PKC-epsilon', 'PKC'] @entity14719 :: ('MESH:C051844', 'Chemical') :: ['nor-binaltorphimine'] @entity3460 :: ('MESH:D014343', 'Chemical') :: ['trypan blue'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity209 :: ('MESH:D008659', 'Disease') :: ['metabolic inhibition preconditioning', 'MIP'] @entity204 :: ('MESH:D003643', 'Disease') :: ['cell injury/death', 'injury/death', 'death']
@entity35 :: (10116,Species) :: ['rat']
