Circadian rhythms are recurring near-twenty four hour patterns driven by an endogenous circadian timekeeping system. The master pacemaker in this system is the hypothalamic @entity10789 ( @entity10789 ). Recently interest has been drawn to how the @entity10789 clock responds to immune system stimulation. A major signalling component in the immune system is nuclear factor (NF)-kB. In the present study we examined the role of NF-kB in @entity10789 function. Whilst serum shocked fibroblasts showed rhythmic nuclear localisation of @entity1992 and @entity1992 -dependent transcription, there were no circadian changes in the @entity10789 in expression of the NF-kB components @entity1992 , @entity16181 , p-IkB or p-IKK. Chronic treatment with the NF-kB inhibitor @entity17727 did not impact on circadian or diurnal rhythms. Phase-shifting light pulses did not impact on @entity10789 expression of @entity1992 , and @entity17727 treatment did not attenuate the behavioural or molecular response to light pulses. Peripheral treatment with lipopolysaccharide resulted in increased NF-kB component expression in the @entity10789 . In vitro experiments with @entity10789 slice cultures showed that treatment with NF-kB inhibitors did not markedly alter rhythmic changes in @entity17989 ::LUC expression. Further, @entity10789 slices from nf-kb::luc @entity19 did not show any evidence for circadian rhythms in NF-kB-mediated transcription. Experiments utilising older @entity19 (~16 months old) showed that @entity10789 treatment in vitro with @entity17727 resulted in increased amplitude of rhythmic @entity17989 ::LUC expression, and @entity558 treatment resulted in altered @entity17989 ::LUC rhythm acrophase. Overall, we interpret our results as providing evidence for the involvement of NF-kB in the suprachiasmatic circadian clock following immune stimulation, but not under basal conditions. This article is protected by copyright. All rights reserved.

NF-kB signalling is involved in immune-modulation, but not basal functioning, of the XXXX suprachiasmatic circadian clock.

@entity17989 :: ('18627(Tax:10090)', 'Gene') :: ['PER2'] @entity1992 :: ('19697(Tax:10090)', 'Gene') :: ['p65'] @entity558 :: ('MESH:C536528', 'Disease') :: ['LPS'] @entity10789 :: ('MESH:D002527', 'Disease') :: ['suprachiasmatic nucleus', 'SCN'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity17727 :: ('C020972', 'Chemical') :: ['PDTC'] @entity16181 :: ('19696(Tax:10090)', 'Gene') :: ['c-Rel']
@entity19 :: (10090,Species) :: ['mouse']
