Background. Natural killer (NK) and natural killer T (NKT) cells contribute to the innate host defense but their role in @entity2348 remains controversial. Methods. C57BL/6 @entity19 were infected intratracheally with 5 * 10(5)   cfu of @entity328 . Animals were divided into sham group (Sham); pretreated with isotype control antibody (CON) group; pretreated with anti-asialo @entity11688 antibody (NKd) group; and pretreated with anti- @entity776 monoclonal antibody (NKTd) group before bacterial challenge. Serum and tissue samples were analyzed for bacterial load, cytokine levels, splenocyte apoptosis rates, and cell characteristics by flow cytometry. Splenocyte miRNA expression was also analyzed and survival was assessed. Results. NK cell depletion prolonged survival. Upon inhibition of NKT cell activation, spleen NK (CD3-/NK1.1+) cells increased compared to all other groups. Inhibition of NKT cell activation led to higher bacterial loads and increased levels of serum and splenocyte IFN-y. Splenocyte miRNA analysis showed that @entity46066 and @entity53504 were downregulated, while miR-125a-5p was upregulated, in anti- @entity776 treated animals. These changes were moderate after NK cell depletion. Conclusions. NK cells appear to contribute to mortality in @entity2258 . Inhibition of NKT cell activation resulted in an increase in spleen NK (CD3-/NK1.1+) cells and a higher IFN-y production, while altering splenocyte miRNA expression.

NK and NKT Cell Depletion Alters the Outcome of XXXX : Relationship with Regulation of Interferon-y Production.

@entity53504 :: ('387222(Tax:10090)', 'Gene') :: ['miR-29a'] @entity776 :: ('12479(Tax:10090)', 'Gene') :: ['CD1d'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity2348 :: ('MESH:D001424', 'Disease') :: ['bacterial sepsis'] @entity11688 :: ('210582(Tax:10090)', 'Gene') :: ['GM1'] @entity2258 :: ('MESH:D011018', 'Disease') :: ['pneumococcal pneumonia'] @entity46066 :: ('723944(Tax:10090)', 'Gene') :: ['miR-200c'] @entity328 :: ('1313', 'Species') :: ['Streptococcus pneumoniae']
@entity2258 :: (MESH:D011018,Disease) :: ['Experimental Pneumococcal Pneumonia']
