The aim of this work was the partial purification and subsequent evaluation of chitinase expression during the various growth phases of @entity12267 . Initially, PbCTS1r was expressed as a recombinant protein and displayed enzymatic activity against 4-MU-[N-acetylglucosamine ( @entity4826 )]3 and @entity61878 2. Two proteins, 45 kDa and 39 kDa in size, were partially purified from @entity12267 @entity375 crude extract using cation-exchange chromatography coupled with HPLC and were characterised as PbCTS1 and PbCTS2, respectively. Anti-PbCTS1r antibody recognised two proteins in the crude extracts of @entity375 and the transitional stage between mycelial and @entity375 phases. In crude extracts of mycelium, only the 45 kDa protein was detected. However, quantitative real-time polymerase chain reaction led to the detection of small quantities of Pbcts2 transcript in the mycelial phase. In the @entity375 cell wall extract, only the 39 kDa protein was detected. Moreover, both proteins were secreted by the @entity375 parasitic phase, suggesting that these proteins participate in the modulation of the fungal environment. Phylogenetic analysis of the predicted PbCTS1 and PbCTS2 proteins indicated that they code for distinct chitinases in @entity12267 . During evolution, @entity12267 could have acquired the paralogues Pbcts1 and Pbcts2 for growth and survival in diverse environments in both saprophytic and parasitic phases.

Distinct chitinases are expressed during various growth phases of the human pathogen XXXX .

@entity4826 :: ('CHEBI:506227', 'Chemical') :: ['GlcNAc'] @entity61878 :: ('MESH:C025158', 'Chemical') :: ['4-MU-(GlcNAc)'] @entity375 :: ('4932', 'Species') :: ['yeast'] @entity12267 :: ('121759', 'Species') :: ['Paracoccidioides brasiliensis', 'P. brasiliensis']
@entity12267 :: (121759,Species) :: ['Paracoccidioides brasiliensis']
