UNASSIGNED: In this study, we developed a novel technique of irreversible @entity1690 by pudendal nerve transection (PNT) using 40 female @entity35 for studying the pathophysiology of stress @entity322 associated with childbirth. Of the 40 @entity35 , 10 served as controls and the remaining underwent bilateral PNT at the anastomotic lumbosacral trunk level. Urethral morphological changes following bilateral PNT were assessed with serial @entity1710 and @entity1711 (H_E) and immunohistochemistry (IHC) staining methods at 50, 90, and 130 days post intervention. Leak point pressure (LPP) measurement was used to determine the effect of @entity130 on urethral outlet resistance after the transection. H_E and IHC staining showed irreversible loss of striated muscle mass of the sphincter region and increase in collagen deposition compatible with @entity2057 . LPP measurements also significantly decreased following bilateral PNT. In conclusion, a novel method of irreversible @entity1887 was developed. This model effectively decreased urethral outlet resistance and caused irreversible striated @entity2057 . We suggest that this technique can be used to develop a permanent @entity1690 model for the preclinical testing of treatment modalities exclusively triggering the pudendal nerve. This article is protected by copyright. All rights reserved.

A novel method of urinary @entity1690 : Serial histopathology evaluation in a @entity35 model of XXXX .

@entity1690 :: ('MESH:D046628', 'Disease') :: ['sphincter deficiency'] @entity322 :: ('MESH:D014549', 'Disease') :: ['urinary incontinence'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity1887 :: ('MESH:D000309', 'Disease') :: ['sphincter insufficiency'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hematoxylin'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity2057 :: ('MESH:D009133', 'Disease') :: ['muscle atrophy'] @entity130 :: ('MESH:D014947', 'Disease') :: ['pudendal injury']
@entity322 :: (MESH:D014549,Disease) :: ['urinary incontinence']
