@entity1566 produces prominent disruption of the blood-spinal cord barrier. We have defined the blood-spinal cord barrier breakdown to the protein luciferase (61 kDa) in the acutely injured @entity19 spinal cord and during revascularization. We show that newly formed and regenerating blood vessels that have abnormal permeability exhibit differential expression of the @entity413 -1 transporter ( @entity29408 ), and that its expression is dependent on astrocytes. There was overt extravasation of luciferase within the first hour after injury, a period that coincided with marked tissue disruption within the epicenter of the lesion. Although there was a significant reduction in the number of blood vessels relative to controls by 24 hr after injury, abnormal barrier permeability remained significantly elevated. A second peak of abnormal barrier permeability at 3-7 days postinjury coincided with prominent revascularization of the epicenter. The barrier to luciferase was restored by 21 days postinjury and vascularity was similar to that of controls. During wound-healing process, the cord was reorganized into distinct domains. Between 14 and 21 days postinjury, each domain consisted primarily of nonneuronal cells, including macrophages. Astrocytes were limited characteristically to the perimeter of each domain. Only blood vessels affiliated closely with astrocytes in the perimeter expressed @entity29408 , whereas blood vessels within each domain of the repairing cord did not express it. Together, these data demonstrate that both injured and regenerating vessels exhibit abnormal permeability and suggest that @entity29408 expression during revascularization is dependent on the presence of astrocytes.

Blood-spinal cord barrier after XXXX : relation to revascularization and wound healing.

@entity1566 :: ('MESH:D013119', 'Disease') :: ['Spinal cord injury'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity29408 :: ('20525(Tax:10090)', 'Gene') :: ['Glut-1']
@entity1566 :: (MESH:D013119,Disease) :: ['spinal cord injury']
