BACKGROUND: In this study, we tested the hypothesis that @entity3009 (PG) receptor expression in the @entity35 kidney is subject to physiological regulation by dietary salt intake. METHODS: @entity35 were fed diets with 0.02 or 4% @entity1219 for two weeks. PG receptor expression was assayed in kidney regions and cells by ribonuclease protection assay and reverse transcription-polymerase chain reaction analysis. Functional correlates were studied by measurement of @entity1421 -induced @entity268 formation and @entity3449 secretion in juxtaglomerular (JG) cells isolated from animals on various salt intakes. RESULTS: @entity10571 and @entity10570 receptors were predominantly expressed, and the EP2 receptor was exclusively expressed in the @entity35 kidney medulla. The @entity23408 receptor was strongly expressed in glomeruli and in @entity3449 -secreting JG granular cells. IP receptor transcripts were found mainly in cortex. Maintaining @entity35 on a low- or high- @entity1219 diet did not affect the expression of @entity10571 or IP receptors, whereas @entity23408 transcripts in glomeruli were increased twofold by salt deprivation. Consistent with this, we found that @entity1421 -evoked @entity268 production and @entity3449 secretion by JG cells from salt-deprived animals were significantly higher compared with cells obtained from salt-loaded animals. In the outer medulla, @entity10570 transcripts correlated directly with salt intake, and mRNA abundance was increased twofold by a high- @entity1219 diet. CONCLUSIONS: Our results suggest that subtype-specific, regional changes in PG receptor expression are involved in the renal adaptation to changes in salt intake. The results are in accord with the general concept that renocortical @entity1421 stimulates @entity3449 secretion and maintains renal blood flow during low-salt states, whereas medullary @entity1421 promotes salt excretion in response to a high salt intake.

Differential regulation of renal prostaglandin receptor mRNAs by dietary salt intake in the XXXX .

@entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity23408 :: ('84023(Tax:10116)', 'Gene') :: ['EP4'] @entity10571 :: ('25637(Tax:10116)', 'Gene') :: ['EP1'] @entity1219 :: ('CHEBI:26710', 'Chemical') :: ['NaCl'] @entity1421 :: ('MESH:D015232', 'Chemical') :: ['PGE2'] @entity3009 :: ('CHEBI:26333', 'Chemical') :: ['prostaglandin'] @entity3449 :: ('24715(Tax:10116)', 'Gene') :: ['renin'] @entity35 :: ('10116', 'Species') :: ['rat', 'Rats', 'rats'] @entity10570 :: ('24929(Tax:10116)', 'Gene') :: ['EP3']
@entity35 :: (10116,Species) :: ['rat']
