We investigated the temporal course of microglia activation in different brain regions after permanent @entity2500 in @entity19 and compared this microglia response with the appearance of apoptotic cells, Microglia activation and morphological changes of microglial cells were visualized using an immunohistochemical method with a polyclonal antibody recognizing the @entity19 CR3 complement receptor. Cells showing morphological and biochemical features of apoptosis were identified using the terminal deoxynucleotidyl transferase nick end-labeling (TUNEL) method and light microscopy. As early as 30 min after onset of @entity2500 activated microglia with @entity1184 cell bodies and stout processes were detected in the periphery of the @entity2806 as identified by diffusion-weighted magnetic resonance imaging. A wider distribution and a progressive increase in the number of activated microglia was found with increasing time. Only few TUNEL-positive cells with apoptotic features were observed within the lesion area at 6 h after onset of @entity2806 . From 12 h after @entity2500 onward a tremendous increase in the number of TUNEL-positive cells was found. Within the thalamus from 24 h onward microglia cells with few processes, irregular morphology and fragmented appearance were detected. Microglia activation in the thalamus progressed up to 4 weeks after @entity2500 , but had declined after 90 days. @entity528 in the thalamus as determined by anti-neuronal nuclei immunohistochemistry progressed from 6 days after @entity2500 onward. Only a few TUNEL-positive cells were found in the thalamus. In summary, microglia activation both in the primary @entity75 area and in the secondarily affected thalamus preceded the manifestation of tissue injury. These observations encourage further studies on the role of microglia in focal @entity2806 .

Time course of microglia activation and apoptosis in various brain regions after permanent focal @entity2806 in XXXX .

@entity2806 :: ('MESH:D002545', 'Disease') :: ['ischemic lesion', 'cerebral ischemia'] @entity2500 :: ('MESH:D020244', 'Disease') :: ['MCA occlusion', 'middle cerebral artery (MCA) occlusion'] @entity1184 :: ('MESH:D006984', 'Disease') :: ['hypertrophic'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity75 :: ('MESH:D001927', 'Disease') :: ['cortical lesion'] @entity528 :: ('MESH:D009410', 'Disease') :: ['Neuronal degeneration']
@entity19 :: (10090,Species) :: ['mice']
