Some dental implants are coated with @entity692 (HA), which preferentially binds to bone. Several matrix proteins have an @entity369 - @entity2228 - @entity2574 (RGD) sequence where cells attach via an integrin receptor. We hypothesized that coating an HA surface with an RGD-containing peptide might enhance the attachment and differentiation of osteoblasts. The HA disks (diameter 34 mm, thickness 1 mm) were treated with a solution (50 mM Tris/HCl and 150 mM @entity1219 , pH 7.4) containing the peptide EEEEEEEPRGDT, in which the E repetition exerts a high affinity to HA. After washing with @entity1391 -buffered saline, KUSA/A1 @entity19 osteoblastic cells were inoculated onto the HA surface and cultured. After 30 min, the number of cells attached to the surface was counted. The DNA content and alkaline phosphatase (ALP) activity were measured after 10 days in culture. Expression of bone matrix proteins was also examined by means of reverse transcriptase-polymerase chain reaction at 7 days; the mineralized area of the culture was also evaluated by staining with @entity6033 after 10 days. Treatment with the peptide stimulated cell attachment and increased DNA content and ALP activity. Furthermore, matrix protein expression and mineralized nodule formation were enhanced to a greater extent on the peptide-treated surface than on the nontreated surface. Our results indicate that coating an HA surface with RGD-containing peptide enhances osteoblast attachment and differentiation. This peptide treatment of HA-coated implants may stimulate the osseointegration of the implants.

Enhancement of osteogenesis on XXXX surface coated with synthetic peptide (EEEEEEEPRGDT) in vitro.

@entity2228 :: ('CHEBI:57305', 'Chemical') :: ['glycine'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity1219 :: ('CHEBI:26710', 'Chemical') :: ['NaCl'] @entity6033 :: ('MESH:C004468', 'Chemical') :: ['Alizarin Red S'] @entity692 :: ('MESH:D017886', 'Chemical') :: ['hydroxyapatite'] @entity1391 :: ('CHEBI:26020', 'Chemical') :: ['phosphate'] @entity2574 :: ('CHEBI:22660', 'Chemical') :: ['aspartic acid'] @entity369 :: ('CHEBI:29016', 'Chemical') :: ['arginine']
@entity692 :: (MESH:D017886,Chemical) :: ['hydroxyapatite']
