@entity842 (NO) is synthesized by @entity842 synthases (NOS) and plays an important role in @entity5 growth. In this study, inducible NOS ( @entity3623 ) expression was evaluated by immunohistochemistry in 34 @entity8205 (13 common @entity8205 , six Spitz naevi, and 15 so-called 'dysplastic naevi'), ten cutaneous @entity320 in situ, 50 stage I invasive @entity320 , and eight subcutaneous metastases of @entity320 . In addition, four samples of @entity8205 and four samples of invasive @entity320 were collected in order to perform western blot and northern blot analysis. By immunohistochemistry, @entity8205 never expressed @entity3623 . Among cases of @entity320 in situ, two were negative, seven displayed staining in less than 20% of @entity320 cells, and positivity was observed in 21-50% of @entity320 cells in only one case. @entity3623 expression was detected in 46 out of 50 invasive @entity320 (92%). Among these cases, 18 showed positivity in less than 20% of @entity320 cells, 18 showed positivity in 21-50% of @entity320 cells, and ten showed @entity3623 expression in more than 50% of cells. Statistical analysis revealed a significant difference in @entity3623 expression between @entity8205 and cutaneous @entity320 (p<0.001). In addition, @entity3623 expression was significantly higher in invasive @entity320 than in @entity320 in situ (p=0.01). Among primary cutaneous @entity320 , no significant correlation was found between @entity3623 expression and histopathological parameters (histotype, level, thickness and presence of regression/inflammatory infiltrate) and disease-specific survival. In subcutaneous @entity320 metastases, @entity3623 expression was diffuse in more than 50% of cells. Statistical analysis revealed that subcutaneous @entity320 metastases showed greater @entity3623 immunoreactivity than invasive @entity320 (p=0.02). Molecular analyses confirmed that @entity3623 mRNA and protein were highly expressed in @entity320 samples. In conclusion, @entity3623 was constantly absent in @entity8205 , whereas it was frequently expressed in @entity320 , with up-regulation of the enzyme paralleling @entity5 progression. These data suggest that @entity3623 may play a role in the @entity5 growth. In addition, @entity3623 may be useful as an immunohistochemical marker for @entity5 .

Inducible XXXX synthase expression in benign and malignant cutaneous melanocytic lesions.

@entity5 :: ('MESH:D009369', 'Disease') :: ['malignant transformation of melanocytes and in tumour', 'malignant melanocytic lesions', 'tumour'] @entity8205 :: ('MESH:D009508', 'Disease') :: ['melanocytic naevi'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['Nitric oxide', 'nitric oxide'] @entity3623 :: ('4843', 'Gene') :: ['iNOS'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma', 'melanomas']
@entity842 :: (MESH:D009569,Chemical) :: ['nitric oxide']
