@entity1199 augments the ability of natural killer (NK) cells to kill @entity2800 cells in vitro, and may have a role in the eradication of @entity41 ( @entity41 ) in @entity3052 @entity1 . The ability to enhance lysis of @entity3052 cells without the @entity137 of @entity1199 would be a significant improvement in the use of biologics against @entity3052 . Recent interest in IL-12 suggested that this cytokine might meet these criteria. The aim of this study was to evaluate the ability of IL-12 to enhance the in vitro lysis of the @entity706 cell lines in a standard 51Chromium release assay. Effector cells from normal volunteers were incubated with varying concentrations of IL-12 or @entity1199 for 18-20 h, then the 51Cr-labeled target cells from five different cell lines of @entity3052 origin were added for 4 h. Percent lysis was determined and plotted over four effector:target (E:T) ratios. Our results indicated that IL-12 was able to enhance lysis of all cell lines tested at > or =5 units/ml. When @entity1199 was added to the culture at a low dose along with IL-12, there appeared to be a synergistic effect. Although anti-gamma interferon was able to inhibit the cytolytic potential of effectors activated by IL-12, the lysis could not be completely blocked. Thus, it appears that IL-12 has the ability to stimulate NK lysis indirectly through the induction of gamma interferon as well as an alternate mechanism not related to gamma interferon. Thus, IL-12 may have a beneficial role in the treatment of @entity706 .

Interleukin-12 (IL-12) enhances lysis of XXXX cell lines in vitro.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity41 :: ('MESH:D018365', 'Disease') :: ['MRD', 'minimal residual disease'] @entity706 :: ('MESH:D008223', 'Disease') :: ['non-lymphoid leukemia'] @entity2800 :: ('MESH:D007951', 'Disease') :: ['myeloid leukemia'] @entity3052 :: ('MESH:D015470', 'Disease') :: ['AML'] @entity1199 :: ('3558', 'Gene') :: ['IL-2']
@entity706 :: (MESH:D008223,Disease) :: ['non-lymphoid leukemia']
