@entity12020 ( @entity12020 ) is a profibrotic factor acting downstream and independently of @entity2533 to mediate @entity8 . Although @entity32 is often involved in the initiation and/or progression of @entity82 , the role of inflammatory cytokines in regulation of glomerular @entity12020 expression, cellular proliferation, and extracellular matrix accumulation is unknown. We studied two such cytokines, @entity310 and @entity379 , for their effects on cultured mesangial cells in the presence or absence of @entity2533 , as a model for progressive @entity8 . Short-term treatment with @entity310 , like @entity2533 , significantly increased secreted @entity12020 per cell, but unlike @entity2533 inhibited cellular replication. @entity310 combined with @entity2533 further increased @entity12020 secretion and mRNA levels and reduced proliferation. Surprisingly, however, @entity310 treatment decreased baseline collagen type I protein and mRNA levels and largely blocked their stimulation by @entity2533 . Long-term treatment with @entity2533 or @entity310 alone no longer increased @entity12020 protein levels. However, the combination synergistically increased @entity12020 . @entity379 had no effect on either @entity12020 or collagen activity and produced a mild inhibition of @entity2533 -induced collagen only at a high concentration (500 U/ml). In summary, we report a strong positive regulatory role for @entity310 , but not @entity379 , in @entity12020 production and secretion, including that driven by @entity2533 . The stimulation of @entity12020 release by @entity310 , unlike @entity2533 , is independent of cellular proliferation and not linked to increased collagen type I accumulation. This suggests that the paradigm of @entity2533 -driven @entity12020 with subsequent collagen production may be overridden by an as yet undefined inhibitory mechanism acting either directly or indirectly on matrix metabolism.

@entity310 , but not @entity379 , regulates XXXX ( @entity12020 ), collagen type I, and proliferation in mesangial cells: possible roles in the progression of @entity8 .

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity8 :: ('MESH:D007674', 'Disease') :: ['renal fibrosis'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis'] @entity310 :: ('7124', 'Gene') :: ['TNF-alpha'] @entity2533 :: ('7040', 'Gene') :: ['TGF-beta'] @entity379 :: ('3458', 'Gene') :: ['IFN-gamma'] @entity12020 :: ('1490', 'Gene') :: ['CCN2', 'Connective tissue growth factor']
@entity12020 :: (1490,Gene) :: ['CCN2']
