UNASSIGNED: The @entity2125 - @entity12931 oxidoreductase [complex I (CI), EC 1.6.5.3] of the mitochondrial respiratory chain is the principal entry point of electrons, and vital in maintaining metabolism and the redox balance. In a variety of eukaryotic organisms, except animal and fungi (Opisthokonta), it contains an extra domain composed of putative gamma carbonic anhydrases subunits, named the CA domain, which was proposed to be essential for complex I assembly. There are two kinds of carbonic anhydrase subunits: CAs (of which there are three) and @entity5846 ( @entity5846 ) (of which there are two). In plants, the CA domain has been linked to photorespiration. In this work, we report that @entity958 mutant plants affected in two specific CA subunits show a lethal phenotype. Double homozygous knockouts ca1ca2 embryos show a significant @entity245 compared to the non-homozygous embryos, which show a @entity164 ( @entity164 ) phenotype in the same silique. Mutant embryos show impaired mitochondrial membrane potential and mitochondrial reactive @entity26 species (ROS) accumulation. The characteristic embryo greening does not take place and fewer but larger oil bodies are present. Although seeds look dark brown and wrinkled, they are able to germinate 12 d later than @entity164 seeds. However, they die immediately, most likely due to oxidative stress.Since the CA domain is required for complex I biogenesis, it is predicted that in ca1ca2 mutants no complex I could be formed, triggering the lethal phenotype. The in vivo composition of a functional CA domain is proposed.

The CA domain of the respiratory complex I is required for normal embryogenesis in XXXX .

@entity2125 :: ('MESH:D009243', 'Chemical') :: ['NADH'] @entity12931 :: ('MESH:D014451', 'Chemical') :: ['ubiquinone'] @entity958 :: ('3702', 'Species') :: ['Arabidopsis'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity245 :: ('MESH:D002658', 'Disease') :: ['developmental delay'] @entity164 :: ('MESH:D017827', 'Disease') :: ['WT', 'wild-type'] @entity5846 :: ('MESH:C536058', 'Disease') :: ['carbonic anhydrase-like proteins', 'CALs']
@entity958 :: (3702,Species) :: ['Arabidopsis thaliana']
