UNASSIGNED: The dorsal root ganglion (DRG) and its anatomically and functionally associated spinal nerve and ventral and dorsal roots are important components of the peripheral sensory-motor system in mammals. The cells within these structures use a number of peptides as intercellular signaling molecules. We performed a variety of @entity3928 ( @entity3928 )-based characterizations of peptides contained within and secreted from these structures, and from isolated and cultured DRG cells. Liquid chromatography-Fourier transform @entity3928 was utilized in DRG and nerve peptidome analysis. In total, 2724 peptides from 296 proteins were identified in tissue extracts. Neuropeptides are among those detected, including @entity10132 , little SAAS, and known hemoglobin-derived peptides. Solid phase extraction combined with direct matrix-assisted laser desorption/ionization time-of-flight @entity3928 was employed to investigate the secretome of these structures. A number of peptides were detected in the releasate from semi-intact preparations of DRGs and associated nerves, including neurofilament- and @entity2610 -related peptides. A smaller set of analytes was observed in releasates from cultured DRG neurons. The peptide signals observed in the releasates have been mass-matched to those characterized and identified in homogenates of entire DRGs and associated nerves. This data aids our understanding of the chemical composition of the @entity1 peripheral sensory-motor system, which is involved in key physiological functions such as nociception, thermoreception, itch sensation, and proprioception.

Peptidomics and Secretomics of the XXXX Peripheral Sensory-Motor System.

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity3928 :: ('MESH:C536030', 'Disease') :: ['mass spectrometry', 'MS'] @entity2610 :: ('4155', 'Gene') :: ['myelin basic protein'] @entity10132 :: ('796', 'Gene') :: ['calcitonin gene-related peptide I']
@entity1 :: (9606,Species) :: ['Mammalian']
