BACKGROUND: Cyclooxygenases (COX) play a key role in prostaglandin metabolism and are important for @entity5 development and progression. The aim of this study was to analyze the prognostic impact of @entity2247 expression in a cohort of lymph node-negative @entity0 @entity1 not treated in the adjuvant setting. METHODS: @entity2247 expression was determined by immunohistochemistry (IHC) in @entity5 tissue of 193 node-negative @entity0 @entity1 . Additionally, mRNA expression was determined in corresponding @entity5 samples using microarray based gene-expression data. Univariate and multivariate Cox regression analyses adjusted for age at diagnosis, @entity5 size, histological grade, @entity1 epithelial growth factor receptor 2 ( @entity1722 ), estrogen receptor (ER) and @entity7273 ( @entity7273 ) were performed to evaluate the association of both @entity2247 protein and mRNA expression with survival. Survival rates were determined by the Kaplan-Meier method. Correlations between @entity2247 expression and established prognostic factors were analyzed using the Chi-square test. A potential correlation between @entity2247 protein expression and @entity2247 mRNA expression was assessed utilizing the Kruscal-Wallis-H-test. RESULTS: @entity2247 protein expression was positive in 24.9% of the @entity0 samples. Univariate analysis showed that @entity2247 protein expression was associated with shorter disease-free survival (DFS) (P   =   0.0001), @entity3 ( @entity3 ) (P   =   0.002) as well as @entity0 specific @entity1349 ( @entity1349 ) (P   =   0.043). In multivariate analysis @entity2247 expression retained its significance independent of established prognostic factors for shorter DFS (P   <   0.001, HR   =   2.767, 95% CI   =   1.563-4.901) and for inferior @entity3 (P   =   0.002, HR   =   2.7, 95% CI   =   1.469-5.263) but not for @entity1349 (P   =   0.096, HR   =   1.929, 95% CI   =   0.889-4.187). In contrast, @entity2247 mRNA expression was not related to survival and failed to show a correlation with protein expression (P   =   0.410). CONCLUSIONS: The present findings support the hypothesis that @entity2247 protein but not mRNA expression is associated with an unfavorable outcome in node-negative @entity0 .

Prognostic influence of cyclooxygenase-2 protein and mRNA expression in node-negative @entity0 XXXX .

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity7273 :: ('5241', 'Gene') :: ['PR', 'progesterone receptor'] @entity3 :: ('MESH:D009362', 'Disease') :: ['MFS', 'metastasis-free survival'] @entity1349 :: ('MESH:D011475', 'Disease') :: ['OS', 'overall survival'] @entity2247 :: ('4513', 'Gene') :: ['COX-2'] @entity1722 :: ('2064', 'Gene') :: ['HER2']
@entity1 :: (9606,Species) :: ['patients']
