The Disk Retention Assay (DRA) is an in vitro method developed to measure the available level of @entity4410 ( @entity4411 ) in mouthwash formulations. This method is based on the binding of the cationic @entity4411 molecule to the anionic surface of a cellulose filter disk. Aqueous @entity4411 solutions demonstrate a linear response (A545) for concentrations up to 0.3%. Higher levels of @entity4411 showed no increased response in the assay. Among common oral product ingredients, at relevant concentrations, surfactants are the primary compounds which inhibit @entity4411 detection and hence, chemical availability. @entity12185 decreased @entity4411 availability to 60% at 0.1%, to 10% at 0.5%, and to 24-33% for 0.2-0.4%. @entity6496 -80 decreased @entity4411 availability to 30% at 0.1% and 6% at 0.25%. A range (4-54%) of available levels of @entity4411 were determined for several commercial products containing 0.045-0.05% nominal levels of @entity4411 indicating significant formulation excipient influence. A plaque glycolysis (PG) assay was used to determine the biological activity of all mouthwash products analyzed by DRA. An experimental series of mouthwash formulations having nominal @entity4411 levels of 0-0.10% demonstrated a good correlation (r2 = 0.955) between the calculated available level of @entity4411 (DRA) and inhibition of plaque glycolysis. The calculated available level of @entity4411 from select commercial mouthwash products, also fit the established correlation with biological activity. The combination of DRA and plaque glycolysis methods are valuable tools which can be used during development to maximize the biological activity of @entity4411 mouthwash formulations prior to clinical evaluation.

Evaluation of XXXX -Containing Mouthwashes Using In Vitro Disk Retention and Ex Vivo Plaque Glycolysis Methods.

@entity4410 :: ('MESH:D002594', 'Chemical') :: ['cetylpyridinium chloride'] @entity4411 :: ('MESH:C015101', 'Chemical') :: ['CPC'] @entity6496 :: ('CHEBI:53426', 'Chemical') :: ['Polysorbate'] @entity12185 :: ('MESH:D020442', 'Chemical') :: ['Poloxamer-407']
@entity4410 :: (MESH:D002594,Chemical) :: ['Cetylpyridinium Chloride']
