AIMS: In @entity28 , infiltration of adipose tissue by proinflammatory immune cells causes chronic low-grade @entity32 . We investigated the chemokine profiles of @entity1 visceral adipocytes by the reverse transcription polymerase chain reaction and the effect of @entity1 neutrophil elastase (HNE) on @entity2842 ( @entity2842 ) mRNA and protein levels. MAIN METHODS: @entity1 adipocytes were obtained from cryopreserved omental preadipocytes of subjects with a body mass index (BMI) <30kg/m(2) or >30kg/m(2) and were cultured to assess chemokine production. KEY FINDINGS: Chemokine responses associated with @entity28 -related @entity32 were well preserved in cultured @entity1 adipocytes derived from cryopreserved preadipocytes. Visceral adipocytes from subjects with a BMI >30kg/m(2) expressed mRNA for @entity2842 , regulated on activation, normal T cell expressed and secreted ( @entity5737 ), @entity18974 ( @entity18974 ), @entity687 ( @entity687 ), lymphotactin-b, and fractalkine. Although visceral adipocytes from subjects with a BMI <30kg/m(2) also expressed @entity2842 , @entity5737 , @entity18974 , and @entity687 mRNA, neither lymphotactin-b nor fraktalkine mRNA was detected. Interestingly, expression of @entity2842 mRNA was decreased significantly after exposure to HNE (85*10(3) M/L), suggesting the induction of nuclear factor-kappa B repressing factor. SIGNIFICANCE: Adipocytes from subjects with a BMI >30kg/m(2) or <30kg/m(2) have different chemokine profiles. Only adipocytes from subjects with a BMI >30kg/m(2) express lymphotactin-b and fractalkine mRNA. Differential chemokine profiles of visceral adipocytes contribute to infiltration of adipose tissue by adaptive immune cells. Neutrophil activation is involved in induction of nuclear factor-kappa B repressing factor, resulting in regulation of immune cell trafficking.

Chemokine profiles of XXXX visceral adipocytes from cryopreserved preadipocytes: Neutrophil activation and induction of nuclear factor-kappa B repressing factor.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity5737 :: ('6352', 'Gene') :: ['RANTES'] @entity28 :: ('MESH:D009765', 'Disease') :: ['obesity'] @entity18974 :: ('6374', 'Gene') :: ['ENA-78', 'epithelial cell-derived neutrophil-activating peptide-78'] @entity2842 :: ('6347', 'Gene') :: ['MCP-1', 'monocyte chemoattractant protein-1'] @entity687 :: ('3576', 'Gene') :: ['interleukin-8', 'IL-8']
@entity1 :: (9606,Species) :: ['human']
