BACKGROUND: @entity2946 ( @entity2946 ) is the most common @entity1313 with the propensity to disseminate at an early stage, and is associated with high morbidity. New treatment strategies are needed to improve cure rates and to reduce @entity73 associated with current therapies. Extracellular Vesicles (EVs) are important players in cell-to-cell communication in health and diseases. A clearer understanding of cell-to-cell communication in @entity5 can be achieved by studying EV secretion in medullospheres. This can reveal subtle modifications induced by the passage from adherent to non-adherent growth, as spheres may account for the adaptation of @entity5 cells to the mutated environment. METHODS: Formation of medullospheres from @entity2946 cell lines stabilized in adherent conditions was obtained through culture conditioning based on @entity258 and specialized medium. EVs collected by ultracentrifugation, in adherent conditions and as spheres, were subjected to electron microscopy, NanoSight measurements and proteomics. RESULTS: Interestingly, @entity68 carrier proteins were only found in EVs shed by CSC-enriched @entity5 cell population of spheres. We used @entity68 chelators when culturing @entity2946 cell lines as spheres. @entity68 chelators induced a decrease in number/size of spheres and in stem cell populations able to initiate in vitro spheres formation. CONCLUSIONS: This work suggests a not yet identified role of @entity68 metabolism in @entity2946 progression and invasion and opens the possibility to use chelators as adjuvants in anti-tumoral chemotherapy.

Proteomic analysis of extracellular vesicles from medullospheres reveals a role for XXXX in the @entity5 .

@entity1313 :: ('MESH:D001932', 'Disease') :: ['malignant childhood brain tumor'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity73 :: ('MESH:D003072', 'Disease') :: ['life-long cognitive and functional deficits'] @entity258 :: ('MESH:D009800', 'Disease') :: ['low attachment flasks'] @entity2946 :: ('MESH:D008527', 'Disease') :: ['Medulloblastoma', 'MB'] @entity68 :: ('MESH:D007501', 'Chemical') :: ['iron', 'Iron']
@entity68 :: (MESH:D007501,Chemical) :: ['iron']
