INTRODUCTION: Disruption of the matrix of endodontic biofilms will aid in their removal from a root canal. Therefore, the aim of this study was to investigate the efficacy of @entity1098 and a @entity1702 ( @entity1702 ) to detach bacteria from biofilms. METHODS: Forty-eight-hour-old @entity797 biofilms were grown on glass coverslips and then treated for 1  hour by immersion in 17% @entity1098 or @entity1702 . @entity4613 -buffered saline served as a negative control. Then, residual biofilm cells on the substrate and the detached cells in the supernatant were collected. Viability was verified by the colony-forming unit (CFU) counting method. @entity15777 ( @entity4515 ) treatment in conjunction with quantitative polymerase chain reaction (qPCR) was also performed to detect the presence of @entity797 16S ribonucleic RNA genes. Data were analyzed using 1-way analysis of variance and Tukey or Kruskal-Wallis and Dunn tests. The Pearson R test evaluated the correlation between results from CFU and @entity4515 (a  =  5%). RESULTS: qPCR showed that @entity1098 detached 99% of biofilm cells, and @entity1702 detached 94% of biofilm cells (both P  <  .001). In contrast to @entity1098 , @entity1702 was highly antimicrobial. The treatment promoted an ample log 7 reduction of the attached cells (P  <  .001), and almost no live cells were detected in the supernatant (P  <  .001). Positive correlations between CFU and qPCR with @entity4515 were observed (r  =  0.959 and r  =  0.729). CONCLUSIONS: @entity1098 detached cells in biofilms with a minor antimicrobial effect. Besides a great antimicrobial effect, @entity1702 also detached biofilm cells. These dispersals of biofilms give insights into new endodontic biofilm removal strategies.

Effectiveness of @entity1098 and Modified Salt Solution to Detach and Kill Cells from XXXX Biofilm.

@entity4613 :: ('D010710', 'Chemical') :: ['Phosphate'] @entity4515 :: ('CHEBI:60755', 'Chemical') :: ['PMA'] @entity1702 :: ('MESH:D013651', 'Disease') :: ['MSS', 'modified salt solution'] @entity797 :: ('1351', 'Species') :: ['Enterococcus faecalis', 'E. faecalis'] @entity15777 :: ('MESH:C533957', 'Chemical') :: ['Propidium monoazide'] @entity1098 :: ('MESH:D004492', 'Chemical') :: ['EDTA']
@entity797 :: (1351,Species) :: ['Enterococcus faecalis']
