UNASSIGNED: The gut microbiota influences many aspects of host metabolism. We have previously shown that the presence of a gut microbiota remodels lipid composition. Here we investigated how interaction between gut microbiota and dietary lipids regulates lipid composition in liver and plasma, and gene expression in liver. Germ-free and conventionally raised @entity19 were fed lard or fish oil diet for 11 weeks. We performed lipidomics analysis of liver and serum and microarray analysis of liver. As expected, most of the variation in the lipidomics dataset was induced by the diet and abundance of most lipid classes differed between @entity19 fed lard and fish oil. However, the gut microbiota also affected lipid composition. The gut microbiota increased hepatic levels of @entity165 and @entity8557 in @entity19 fed lard but not in @entity19 fed fish oil. Serum levels of @entity165 and @entity8557 were not affected by the gut microbiota. Genes encoding enzymes involved in @entity165 biosynthesis were downregulated by the gut microbiota in @entity19 fed lard and were expressed at a low level in @entity19 fed fish oil independent of microbial status. In summary, we show that gut microbiota-induced regulation of hepatic @entity165 metabolism is dependent on dietary lipid composition.

Interaction between dietary lipids and gut microbiota regulates hepatic XXXX metabolism.

@entity19 :: ('10090', 'Species') :: ['mice'] @entity8557 :: ('MESH:D002788', 'Chemical') :: ['cholesteryl esters'] @entity165 :: ('MESH:D002784', 'Chemical') :: ['cholesterol']
@entity165 :: (MESH:D002784,Chemical) :: ['cholesterol']
