Changes in the proportion of peripheral blood T cell subsets after subcutaneous inoculation of @entity291 with @entity2497 Bacille Calmette-Guerin (BCG) were studied. @entity291 were injected with approximately 8 x 10(6) BCG bacillus and blood samples collected at weekly intervals for flow-cytometric analyses to determine the proportion of @entity9214 +, CD8+ and gammadelta T cells. In addition, whole blood samples were stimulated in vitro with @entity2499 purified protein derivative (PPD) and the secreted @entity12028 quantified by ELISA. Results showed cellular and cytokine changes which could be categorized into three phases. The first phase occurred within the first 2 weeks after vaccination involving an increase in proportion of @entity9777 + gammadelta T cells and a concomitant increase in the secretion of @entity12028 . These two responses peaked at 2 weeks and waned thereafter. The second phase involved an increase in the @entity9214 /CD8 ratio as a result of an increase in the proportion of @entity9214 + T cells between 4 and 6 weeks. The third phase involved a decrease in the @entity9214 /CD8 ratio due to an increase in the proportion of CD8+ T cells between 8 and 10 weeks. Surprisingly, the @entity12028 response was associated with changes in the gammadelta rather than the @entity9214 + or CD8+ T cells, suggesting that this cytokine was secreted by gammadelta-T cells. These results are consistent with the reported ability of gammadelta T cells to act rapidly and bridging the innate and classically adaptive immune responses.

Vaccination of XXXX with Mycobacteria @entity2497 Bacilli Calmete Guerin (BCG) induced rapid increase in the proportion of peripheral blood gammadelta T cells.

@entity9777 :: ('751809(Tax:9913)', 'Gene') :: ['WC1'] @entity12028 :: ('281237(Tax:9913)', 'Gene') :: ['IFN-gamma'] @entity2499 :: ('28903', 'Species') :: ['M. bovis'] @entity9214 :: ('407098(Tax:9913)', 'Gene') :: ['CD4'] @entity291 :: ('9913', 'Species') :: ['Calves', 'cattle'] @entity2497 :: ('1765', 'Species') :: ['Mycobacterium bovis']
@entity291 :: (9913,Species) :: ['calves']
