Bone marrow-derived dendritic cell (BMDC) subsets have distinct immunoregulatory functions. @entity630 cytokine-induced BMDC (BMDC1), compared with @entity61 cytokine-induced BMDC2, have superior activities for the differentiation and expansion of CTL. To evaluate the cellular interactions between dendritic cells and CD8+ T cells for the induction of CTL, BALB/c-derived BMDC subsets were cocultured with purified CD8+ T cells from C57BL/6 @entity19 . Our results demonstrate that BMDC1 support the generation of allogeneic CD8+ CTL in the absence of @entity1961 + Th cells. In contrast, BMDC0 ( @entity2304 - plus @entity3019 -induced BMDC) and BMDC2 failed to promote the differentiation of CD8+ CTL. Using Ab-blocking experiments and studies with gene knockout @entity19 , @entity2047 and @entity12213 are demonstrated to be critical for BMDC1-induced CTL differentiation. Unexpectedly, BMDC1 were able to induce CTL from CD8+ T cells isolated from @entity60 @entity19 . However, BMDC1 produced higher levels of @entity12421 than other BMDC subsets, and anti- @entity12421 mAb blocked BMDC1-dependent CTL generation. These results indicated an indispensable role of @entity12421 , but not @entity60 , during BMDC1-induced CTL differentiation. We conclude that @entity630 -cytokine-conditioned BMDC1 can bypass Th cell function for the differentiation of naive CD8+ T cells into CTL.

XXXX cytokine-conditioned bone marrow-derived dendritic cells can bypass the requirement for Th functions during the generation of CD8+ CTL.

@entity2304 :: ('12981(Tax:10090)', 'Gene') :: ['GM-CSF'] @entity2047 :: ('16183(Tax:10090)', 'Gene') :: ['IL-2'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity3019 :: ('16187(Tax:10090)', 'Gene') :: ['IL-3'] @entity60 :: ('15978(Tax:10090)', 'Gene') :: ['IFN-gamma', 'IFN-gamma-/- and IFN-gamma receptor-/-'] @entity12213 :: ('16414(Tax:10090)', 'Gene') :: ['LFA-1'] @entity61 :: ('15111(Tax:10090)', 'Gene') :: ['Th2'] @entity12421 :: ('15977(Tax:10090)', 'Gene') :: ['IFN-beta'] @entity1961 :: ('12504(Tax:10090)', 'Gene') :: ['CD4'] @entity630 :: ('57314(Tax:10090)', 'Gene') :: ['Th1']
@entity630 :: (57314(Tax:10090),Gene) :: ['Th1']
