Butyltin (BT) compounds are known for their worldwide contamination. Dibutyltin (DBT) is used as a stabilizer in plastic products, and as a deworming agent in poultry. Poultry products have been shown to contain measurable levels of DBT. Drinking water has also been reported to contain BTs due to leaching from PVC pipes. We, and others, have found measurable levels of DBT in @entity1 blood. BTs appear to increase the risk of @entity5 in exposed individuals. In previous studies we have shown that the @entity5 killing function of natural killer (NK) lymphocytes was greatly diminished after as little as a 1 h exposure to DBT and the inhibition continued even after removal of the compound. We also showed that there was a significant decrease in NK cell lysis of K562 target cells after an exposure to 1.5 microM DBT for 24 h. This 24 h exposure also decreased the ability of NK cells to bind to @entity5 cells. Loss of binding function was not seen when NK cells were exposed to 5-10 microM DBT for 1 h. However, NK cells exposed to 5 microM DBT for 1 h and then incubated in DBT-free media for 24, 48, or 96 h, showed a significant loss of @entity5 -binding function within 24 h. The effects of DBT exposure on seven cell surface molecules that are involved in NK-cell interactions with target cells were investigated. The results indicated that the exposure of NK cells to 1.5 microM DBT for 24 h decreased the expression of @entity3138 , @entity1453 , @entity4071 , @entity2935 . There was no decrease in expression of any of the markers studied when NK cells were exposed to 5 microM DBT for 1 h, consistent with the fact that a 1-h exposure had no effect on the ability of NK cells to bind @entity5 cells. However, when NK cells were exposed to 5 microM DBT for 1 h followed by 24, 48 or 96 h incubations in DBT-free media there was decreased expression of several of the cells surface molecules with the most dramatic decreases being in @entity4071 and @entity2940 .

Expression of functionally relevant cell surface markers in dibutyltin-exposed XXXX natural killer cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity2940 :: ('4684', 'Gene') :: ['CD56'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer and other viral infections'] @entity3138 :: ('914', 'Gene') :: ['CD2'] @entity4071 :: ('2214', 'Gene') :: ['CD16'] @entity1453 :: ('3683', 'Gene') :: ['CD11a'] @entity2935 :: ('3687', 'Gene') :: ['CD11c']
@entity1 :: (9606,Species) :: ['human']
