Target-soluble @entity8816 -sensitive factor attachment protein receptors (t-SNAREs) are receptors that facilitate vesicle and target membrane fusion. Syntaxin 4 is the t-SNARE critical for insulin-stimulated @entity413 transporter 4 ( @entity3240 )-plasma membrane fusion in adipocytes. @entity50304 is a novel IGF-I/insulin-regulated @entity413 transporter expressed in the @entity19 blastocyst. Similar to @entity3240 , @entity50304 translocates to the plasma membrane to increase @entity413 uptake at a stage in development when @entity413 serves as the main substrate. Any decrease in @entity50304 cell surface expression results in increased apoptosis and @entity393 . Previous studies have also shown that disruption of the syntaxin 4 ( @entity51525 ) gene results in early @entity2085 before embryonic d 7.5. We have now demonstrated that syntaxin 4 protein is localized predominantly to the apical plasma membrane of the @entity19 blastocyst. @entity51525 inheritance, as detected by protein expression, occurs with the expected Mendelian frequency up to embryonic d 4.5. In parallel, 22% of the blastocysts from @entity51525 +/- matings had no significant insulin-stimulated translocation of @entity50304 whereas 77% displayed either partial or complete translocation to the apical plasma membrane. This difference in @entity50304 translocation directly correlated with one-third of blastocysts from @entity51525 +/- mating having reduced rates of insulin-stimulated @entity413 uptake and 67% with wild-type rates. These data demonstrate that the lack of syntaxin 4 expression results in abnormal movement of @entity50304 in response to insulin, decreased insulin-stimulated @entity413 uptake, and increased apoptosis. Thus, syntaxin 4 functions as the necessary t-SNARE protein responsible for correct fusion of the @entity50304 -containing vesicle with the plasma membrane in the @entity19 blastocyst.

Syntaxin 4 expression affects XXXX transporter 8 translocation and embryo survival.

@entity2085 :: ('MESH:D020964', 'Disease') :: ['embryonic lethality'] @entity50304 :: ('56017(Tax:10090)', 'Gene') :: ['GLUT8'] @entity19 :: ('10090', 'Species') :: ['mouse', 'murine'] @entity8816 :: ('MESH:D005033', 'Chemical') :: ['N-ethylmaleimide'] @entity51525 :: ('20909(Tax:10090)', 'Gene') :: ['Stx4a'] @entity3240 :: ('20528(Tax:10090)', 'Gene') :: ['GLUT4'] @entity393 :: ('MESH:D034381', 'Disease') :: ['pregnancy loss'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity413 :: (MESH:D005947,Chemical) :: ['glucose']
