The chronic effects of @entity4742 ( @entity4743 ) and @entity2847 on the expression of galanin in the @entity35 anterior pituitary were examined and compared with the expression of prolactin and vasoactive intestinal peptide ( @entity12180 ) after the same type of hormonal manipulation. Total pituitary RNA was subjected to Northern blot analysis 2, 7, and 28 days after @entity35 were implanted sc with a 10-mg DES pellet with or without concurrent treatment with @entity2847 (5 mg/kg per day). @entity172 treatment resulted in a rise in galanin, prolactin, and @entity12180 messenger RNA (mRNA) that was particularly evident for galanin. Coadministration of @entity2847 with DES suppressed the response to @entity172 with galanin, prolactin, and @entity12180 mRNA levels being in general lower than when treatment with @entity172 alone was used. In the combined treatment group there was an increase in the levels for the three mRNA species over the control group. Pituitary content of galanin and prolactin measured by radioimmunoassay exhibited a temporal pattern similar to that of corresponding mRNA levels after administration of @entity172 alone or in combination with @entity2847 . The rise in galanin content (up to 78-fold) was higher than the increase in prolactin immunoreactivity (up to 6.5-fold compared to control). @entity2847 partially suppressed the @entity172 -induced increase in the content of these peptides. The changes in galanin and prolactin peptide levels in the plasma paralleled those found in the pituitary. This study demonstrates that the neuropeptides galanin and @entity12180 are coregulated with prolactin after @entity172 and @entity2847 administration. Since galanin and @entity12180 are known to stimulate prolactin secretion it is possible that they mediate, at least in part, the effects of @entity172 and @entity2847 on pituitary size and/or prolactin release.

@entity2847 inhibits galanin gene expression in the XXXX pituitary gland.

@entity172 :: ('MESH:D004967', 'Chemical') :: ['estrogen', 'Estrogen'] @entity2847 :: ('MESH:D001971', 'Chemical') :: ['bromocriptine', 'Bromocriptine'] @entity4743 :: ('CHEBI:37628', 'Chemical') :: ['DES'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity12180 :: ('117064(Tax:10116)', 'Gene') :: ['VIP'] @entity4742 :: ('MESH:D004054', 'Chemical') :: ['diethylstilbestrol']
@entity35 :: (10116,Species) :: ['rat']
