OBJECTIVES: The purpose of the present study was to investigate leakage of plasma proteins in connection with the inflammatory airway reaction following exposure to dust in a @entity1707 house. Inhalation of @entity1707 -house dust causes intense @entity32 with influx of inflammatory cells, predominantly neutrophils, into the airways. The aim of the study was to compare the concentration of three different proteins in bronchoalveolar lavage (BAL) fluid as markers for the @entity32 . METHODS: In twenty healthy, non-allergic, non-smokers, not previously exposed to farm dust, BAL was performed approximately 2 weeks before and 24 h after 3 h of exposure to @entity1707 dust in a @entity1707 -confinement building. Differential cell count and protein concentration were assessed in BAL fluid. @entity63357 (66.5 kDa) and @entity70335 (720 kDa) were quantified by the use of enzyme-linked immunosorbent assay (ELISA) techniques, and @entity70336 (80 kDa) by zone immunoelectrophoresis assay. The coefficient of variation for repeated protein measurements was <9%. RESULTS: @entity70335 concentration increased six-fold, from 68.0 (36.1-99.9) microg/l, mean (95% CI) before exposure to 411.2 (254.0-568.4) microg/l after exposure (P < 0.001). @entity70336 and @entity63357 increased from 19.7 (16.2-23.1) mg/l and 1.8 (1.4-2.2) mg/l, 2.6 and 1.9 times, respectively (P < 0.001). There was significant correlation between the exposure-induced increased protein levels in BAL fluid, although @entity70335 was a better discriminator of pre- and post-exposure concentrations than were @entity63357 and @entity70336 . There was a significant correlation between the exposure-induced BAL-fluid neutrophilia and the increase in @entity70335 and @entity70336 , but not for @entity63357 . This correlation was found only when pre- and post- differences, but not ratios, of plasma proteins were compared. CONCLUSIONS: The levels of plasma proteins increased in BAL fluid following exposure to @entity1707 -house dust. @entity70335 was a better marker of this plasma leakage than were @entity63357 and @entity70336 .

@entity63357 , @entity70336 and XXXX in bronchoalveolar lavage fluid following exposure to organic dust in healthy subjects.

@entity1707 :: ('9823', 'Species') :: ['pig', 'swine'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity70335 :: ('100153288(Tax:9823)', 'Gene') :: ['alpha2-macroglobulin', 'alpha2-Macroglobulin'] @entity70336 :: ('396996(Tax:9823)', 'Gene') :: ['Transferrin', 'transferrin'] @entity63357 :: ('396960(Tax:9823)', 'Gene') :: ['albumin', 'Albumin']
@entity70335 :: (100153288(Tax:9823),Gene) :: ['alpha2-macroglobulin']
