We have evaluated the use of cytochrome P450 (CYP), 3A4 genotype and Fourier transform-infrared (RT-IR)/Raman spectroscopy as diagnostic tools for detection of @entity0 . CYP is involved in catalytic activity of oxidative metabolism of many chemicals in fatty tissues, and it plays a major role in biotransformation and detoxication of environmental contaminants. FT-IR and Raman spectroscopy have been used to develop methods for @entity5 assessment. Thus, the hypothesis was that a) @entity5322 gene expression level may have effect on the clinical presentation of @entity0 ; and b) a combination spectroscopy and genotype analysis may strengthen the level of diagnosis. In parallel studies we compared by reverse-transcription-polymerase chain reaction (RT-PCR), the @entity5322 mRNA transcript levels, and by FT-IR the pathology of breast tissues. RNA was isolated from @entity1 breast biopsies and cultured @entity5 cells (MCF-7). A comparison of the levels of RT-PCR was made between @entity5322 , a genotype associated with @entity1 @entity5 , testing 3 normal breast tissues, 2 specimen from @entity0 . Two variants of @entity5322 mRNA were observed, of which a 380-bp was displayed in 4 out of 5 pathologically determined @entity5 , and a 260-bp fragment was associated with normal tissues. The predictive value of the @entity5322 for the detection of @entity5 tissues was greater than that observed with the @entity4188 . FT-IR signal patterns were distinct for @entity5 tissues as compared with that of normal tissue. Our findings demonstrated the importance of @entity5322 as molecular biomarker for determining the presence of @entity0 . This data in association with FT-IR/Raman spectroscopy and pathology, it can be an ideal test for predicting the clinical presentation of @entity0 .

Incorporating cytochrome P450 3A4 genotype expression and FT-IR/Raman spectroscopy data as means of identification of XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer', 'breast tumors', 'breast reduction and 7 breast tumors'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'tumors'] @entity5322 :: ('1576', 'Gene') :: ['CYP 3A4', 'CYP 3A4 genotype and 1B1'] @entity4188 :: ('1545', 'Gene') :: ['CYP 1B1']
@entity0 :: (MESH:D001943,Disease) :: ['breast tumors']
