UNASSIGNED: @entity1457 proteins (HSPs) are over-expressed in a wide range of @entity1 @entity5 . It results in the stimulation of the immune system and consequently in elevated concentration of anti-HSP autoantibodies. Elevated anti-HSP autoantibodies were found in @entity0 @entity1 , and they are associated with @entity5 metastasis. Therefore, screening these autoantibodies could be of diagnostic and prognostic values. Protein microarrays have already demonstrated their great potential as a diagnostic tool. However, protein diversity requires optimization of the microarray fabrication to achieve high sensitivity and specificity. In this study, seven HSPs were immobilized on six different surface chemistries. After evaluation and optimization with purified antibodies of the six surface chemistries, two surfaces were selected to detect anti-HSP autoantibodies in @entity0 sera. Multiplex detection of anti-HSP autoantibodies allowed discrimination of @entity0 @entity1 (50) from healthy controls (26) with a sensitivity of 86  % and a specificity of 100  %. Graphical abstract Receiver operating characteristic (ROC) curve analysis for the discrimination between @entity0 @entity1 and healthy controls: detection of individual autoantibody on optimal surface chemistry ( @entity1473 or chitosan surface) and combination (black line) of the detection of 7 autoantibodies.

Anti- @entity1457 protein autoantibody profiling in XXXX using customized protein microarray.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancers'] @entity1457 :: ('MESH:D012769', 'Disease') :: ['Heat shock'] @entity1473 :: ('CHEBI:46883', 'Chemical') :: ['COOH']
@entity0 :: (MESH:D001943,Disease) :: ['breast cancer']
