BACKGROUND: Theoretically @entity1 embryonic stem cells (hESCs) have the capacity to self-renew and differentiate into all @entity1 cell types. Therefore, the greatest promise of hESCs-based therapy is to replace the damaged tissues of @entity1 suffering from traumatic or @entity179 by the exact same type of cells derived from hESCs. Allograft immune rejection is one of the obstacles for hESCs-based clinical applications. Human @entity4107 leads to @entity404 (+) T cells-mediated allograft rejection. Hence, we focus on optimizing hESCs for clinic application through gene modification. RESULTS: Transcription activator-like effector nucleases (TALENs) were used to target MHC class II transactivator ( @entity4637 ) in hESCs efficiently. @entity4637 (-/-) hESCs did not show any difference in the differentiation potential and self-renewal capacity. Dendritic cells (DCs) derived from @entity4637 (-/-) hESCs expressed @entity8730 and @entity2937 but without the constitutive HLA II. Fibroblasts derived from @entity4637 (-/-) hESCs were powerless in IFN-y inducible expression of HLA II. CONCLUSION: We generated HLA II defected hESCs via deleting @entity4637 , a master regulator of constitutive and IFN-y inducible expression of HLA II genes. @entity4637 (-/-) hESCs can differentiate into tissue cells with non-HLA II expression. It's promising that @entity4637 (-/-) hESCs-derived cells could be used in cell therapy (e.g., T cells and DCs) and escape the attack of receptors' @entity404 (+) T cells, which are the main effector cells of cellular immunity in allograft.

Functional disruption of human leukocyte antigen II in XXXX embryonic stem cell.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity404 :: ('920', 'Gene') :: ['CD4'] @entity2937 :: ('942', 'Gene') :: ['CD86'] @entity4637 :: ('4261', 'Gene') :: ['CIITA'] @entity8730 :: ('9308', 'Gene') :: ['CD83'] @entity179 :: ('MESH:D019636', 'Disease') :: ['degenerative diseases'] @entity4107 :: ('3126', 'Gene') :: ['leukocyte antigen (HLA) II']
@entity1 :: (9606,Species) :: ['human']
