UNASSIGNED: Superficial mycoses are limited to the most external part of the skin and hair and caused by Malassezia sp., @entity12734 sp. and Candida sp. We report extracellular biosynthesis of @entity575 nanoparticles (AgNPs) by acidophilic actinobacteria (SF23, C9) and its in  vitro antifungal activity against fungi-causing superficial mycoses. The phylogenetic analysis based on the 16S rRNA gene sequence of strains SF23 and C9 showed that they are most closely related to Pilimelia columellifera subsp. pallida GU269552(T) . The detection of AgNPs was confirmed by visual observation of colour changes from colourless to brown, and UV-vis spectrophotometer analysis, which showed peaks at 432 and 427  nm, respectively. These AgNPs were further characterised by nanoparticle tracking analysis (NTA), Zeta potential, Fourier-transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). The FTIR analysis exhibited the presence of proteins as capping agents. The TEM analysis revealed the formation of spherical and polydispersed nanoparticles in the size range of 4-36  nm and 8-60  nm, respectively. The biosynthesised AgNPs were screened against fungi-causing superficial mycoses viz., Malassezia furfur, @entity12734 , @entity5132 and @entity9841 . The highest antifungal activity of AgNPs from SF23 and C9 against @entity12734 and the least against M.  furfur and @entity5132 was observed as compared to other tested fungi. The biosynthesised AgNPs were found to be potential anti-antifungal agent against fungi-causing superficial mycoses.

Acidophilic actinobacteria synthesised XXXX nanoparticles showed remarkable activity against fungi-causing superficial mycoses in humans.

@entity5132 :: ('5476', 'Species') :: ['Candida albicans', 'C.  albicans'] @entity9841 :: ('5482', 'Species') :: ['C.  tropicalis'] @entity12734 :: ('5551', 'Species') :: ['Trichophyton rubrum', 'T.  rubrum', 'Trichophyton'] @entity575 :: ('MESH:D012834', 'Chemical') :: ['silver']
@entity575 :: (MESH:D012834,Chemical) :: ['silver']
