We investigated the in vivo and in vitro effects of lipopolysaccharide (LPS) treatment (4 mg/kg i.p.) on @entity840 airway smooth muscle reactivity and epithelial bioelectric responses to @entity2710 (MCh) and @entity1602 . Hyperosmolar challenge of the epithelium releases epithelium-derived relaxing factor (EpDRF). Using a two-chamber, whole body plethysmograph 18 h post-treatment, animals treated with LPS were hyporeactive to inhaled MCh aerosol. This could involve an increase in the release and/or actions of EpDRF, because LPS treatment enhanced EpDRF-induced smooth @entity121 in vitro in the isolated perfused @entity825 apparatus. In isolated perfused tracheas the basal transepithelial potential difference (Vt) was increased after LPS treatment. The increase in Vt was inhibited by @entity6478 and @entity3352 . Concentration-response curves for changes in Vt in response to serosally and mucosally applied MCh were biphasic (hyperpolarization, <3 x 10(-7)M; depolarization, >3 x 10(-7)M); MCh was more potent when applied serosally. The hyperpolarization response to MCh, but not the depolarization response, was potentiated after LPS treatment. In both treatment groups, mucosally applied hyperosmolar solution (using added @entity1219 ) depolarized the epithelium; this response was greater in tracheas from LPS-treated animals. The results of this study indicate that airway hyporeactivity in vivo after LPS treatment is accompanied by an increase in the release and/or actions of EpDRF in vitro. These changes may involve LPS-induced bioelectric alterations in the epithelium.

Hyperosmolar solution effects in @entity840 airways. IV. Lipopolysaccharide-induced alterations in airway reactivity and epithelial bioelectric responses to XXXX and @entity1602 .

@entity825 :: ('MESH:D055090', 'Disease') :: ['trachea'] @entity121 :: ('MESH:D009135', 'Disease') :: ['muscle relaxation'] @entity1219 :: ('CHEBI:26710', 'Chemical') :: ['NaCl'] @entity840 :: ('10141', 'Species') :: ['guinea pig'] @entity6478 :: ('MESH:D000584', 'Chemical') :: ['amiloride'] @entity3352 :: ('MESH:D007213', 'Chemical') :: ['indomethacin'] @entity2710 :: ('MESH:D016210', 'Chemical') :: ['methacholine'] @entity1602 :: ('MESH:D006944', 'Disease') :: ['hyperosmolarity']
@entity2710 :: (MESH:D016210,Chemical) :: ['methacholine']
