BACKGROUND: To observe the inhibition effects of the Buthus matensii Karsch (BmK) scorpion venom extracts on the growth of @entity1 @entity0 MCF-7 cells, and to explore its mechanisms. METHODS: Two common @entity5 cells (SMMC7721, MCF-7) were examined for the one which wasmore sensitivity to scorpion venom by @entity98 method. Cell cycle was determined by flow cytometry. Immunocytochemistry was applied to detect apoptosis-related protein @entity522 and @entity588 levels, while the expression of cell cycle-related protein @entity4910 was shown by Western blotting. RESULTS: Our data indicated that MCF-7 was the more sensitive cell line to scorpion venom. The extracts of scorpion venom could inhibit the growth and proliferation of MCF-7 cells. Furthermore, the extract of scorpion venom induced apoptosis through @entity522 up-regulation while @entity588 down-regulation in MCF-7 cells. In addition, the extracts of scorpion venom blocked the cells from G0/G1 phase to S phase and decreased cell cycle-related protein @entity4910 level after drug intervention compared with the negative control group. CONCLUSIONS: These results showed that the BmK scorpion venom extracts could inhibit the growth of MCF-7 cells by inducing apoptosis and blocking cell cycle in G0/G1 phase. The BmK scorpion venom extracts will be very valuable for the treatment of @entity0 .

Inhibition effects of scorpion venom extracts (Buthus matensii Karsch) on the growth of @entity1 XXXX MCF-7 cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity522 :: ('836', 'Gene') :: ['Caspase-3'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity588 :: ('596', 'Gene') :: ['Bcl-2'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity4910 :: ('595', 'Gene') :: ['Cyclin D1'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT']
@entity0 :: (MESH:D001943,Disease) :: ['breast cancer']
