BACKGROUND: Platelet-rich plasma ( @entity16447 ) can provide an assortment of growth factors, but how @entity16447 effects bone regeneration is still unknown. The aim of the study was to explore an optimal method of using @entity16447 and bone marrow stromal cells (BMSCs). METHODS: An in vitro experiment was first conducted to determine an appropriate quantity of @entity16447 . BMSCs were cultured with @entity16447 of different concentrations to assess cell proliferation and osteogenic differentiation. Following the in vitro study, a @entity35 femoral @entity1347 model was used. Five collagen mixtures consisting of different concentrations of @entity16447 and BMSCs were prepared as follows, i) BMSCs and @entity16447 (platelet 20 x 10(4)/  l), ii) BMSCs and @entity16447 (platelet 100 x 10(4)/  l), iii) BMSCs and @entity16447 (platelet 500 x 10(4)/  l), iv) BMSCs, and v) @entity16447 group (platelet 100 x 10(4)/  l), were used to fill defect. New bone formation was evaluated by soft X- @entity16039 and histologic analyses were performed at 2, 4, 6 and 8 weeks postoperatively. RESULTS: The cell proliferation increased @entity16447 concentration-dependently. Cellular alkaline phosphatase activity was higher in moderate concentration than high or low concentration group's in vitro study. In vivo study, the bone fill percentage of newly formed bone in BMSCs and @entity16447 (platelet 100 x 10(4)/  l) was 46.9% at 8 weeks and increased significantly compared with other groups. CONCLUSION: BMSCs with moderate level of @entity16447 significantly enhanced bone formation in comparison with BMSCs or @entity16447 transplant in a @entity35 femoral defect model.

The Bone Regeneration Using Bone Marrow Stromal Cells with Moderate Concentration Platelet-Rich Plasma in Femoral Segmental Defect of XXXX .

@entity16447 :: ('287750(Tax:10116)', 'Gene') :: ['PRP'] @entity1347 :: ('MESH:C537775', 'Disease') :: ['segmental defect'] @entity16039 :: ('255564', 'Species') :: ['ray'] @entity35 :: ('10116', 'Species') :: ['rat']
@entity35 :: (10116,Species) :: ['Rats']
