OBJECTIVES: A nuclear matrix protein ( @entity11620 ) was previously identified and reported to be present only in @entity1 @entity263 but absent in tissue from the same prostate containing either @entity1067 ( @entity1067 ) or normal prostate tissue. The @entity11620 protein was identified by high resolution two-dimensional @entity2975 gel electrophoresis (2D-PAGE) and exhibited a molecular mass of 56 kDa and an isoelectric point of 6.58. This work investigates the immunohistochemical characterization of PRO:4-216, a monoclonal antibody to @entity11620 . METHODS: Areas of the 2D-PAGE gels containing the @entity1 @entity263 nuclear matrix proteins near @entity11620 were isolated, eluted, and injected into @entity19 to develop monoclonal antibodies. Antibodies were screened by immunofluorescence for nuclear reactivity to a @entity1 @entity263 cell line (LnCaP) and by 1D and 2D Western blots for reactivity with @entity263 nuclear matrix proteins. Monoclonal antibodies from the selected clones were affinity purified. The monoclonal antibody PRO:4-216 was used to analyze frozen tissue from 20 cancerous, 22 @entity1067 , and 22 normal regions from fresh @entity1 prostate specimens. Tissue sections were analyzed for their immunohistochemical (IHC) (horseradish peroxidase) staining. RESULTS: Using a reference value for positive staining at an IHC score of greater than 50, 85% (17 of 20) of the cancerous, 5% (1 of 22) of the @entity1067 , and 9% (2 of 22) of the normal prostate tissues stained positive. The one @entity1067 and two normal tissues that stained positive were taken from prostates in which the adjacent cancerous tissue also demonstrated high IHC scores (greater than 225). CONCLUSIONS: These data demonstrate nuclear reactivity on fresh frozen @entity1 @entity263 tissue for the monoclonal antibody PRO:4-216. PRO:4-216 may aid in distinguishing normal prostate and @entity1067 from cancerous tissue.

Preliminary immunohistochemical characterization of a monoclonal antibody (PRO:4-216) prepared from @entity1 XXXX nuclear matrix proteins.

@entity1 :: ('9606', 'Species') :: ['human'] @entity263 :: ('MESH:D011471', 'Disease') :: ['prostate cancer'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity1067 :: ('MESH:D011470', 'Disease') :: ['benign prostatic hyperplasia', 'BPH'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity11620 :: ('5122', 'Gene') :: ['PC-1']
@entity263 :: (MESH:D011471,Disease) :: ['prostate cancer']
