@entity3 is one of the most common complications in @entity2 . However, no effective therapy for @entity3 has been established thus far. We have constructed a metastatic model of @entity1149 ( @entity1149 ) by injecting @entity1 @entity1149 cell lines directly into the left @entity1143 of BALB/c @entity19 . Because this model is easy to construct and the results are reproducible, we used this model for a preclinical evaluation of gene therapy for @entity3 of @entity1149 . We took the novel approach of in vivo lipofection of a suicidal gene to @entity2 cells metastasized to the @entity1143 . A @entity1 @entity2 cell line, PC14, was inoculated into the @entity1143 of @entity19 . After 1 day, a herpes simplex virus @entity1014 kinase gene expression plasmid was injected intrapleurally as a DNA-liposome complex, and @entity4540 was subsequently administered for 8 days. The survival rates of the @entity4540 -treated group were significantly better than those of the control groups. Flow cytometric analysis using a green fluorescent protein expression plasmid suggested that the transfection efficiency in the @entity1143 was 13.6%. Moreover, due to a bystander effect with PC14 cells, 10% of the gene transfer efficiency was sufficient to eradicate or suppress @entity3 . This preclinical study suggests the therapeutic feasibility of an in vivo lipofection-based suicidal gene/prodrug strategy for @entity3 of @entity1149 .

Suicidal gene therapy for XXXX of @entity2 by liposome-mediated transfer of herpes simplex virus @entity1014 kinase gene.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1143 :: ('MESH:D010996', 'Disease') :: ['pleural cavity'] @entity19 :: ('10090', 'Species') :: ['nude mice'] @entity1149 :: ('MESH:D002289', 'Disease') :: ['NSCLC', 'non-small cell lung cancer'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung cancers', 'lung cancer'] @entity3 :: ('MESH:D009362', 'Disease') :: ['pleural metastasis', 'Pleural metastasis'] @entity1014 :: ('MESH:D013936', 'Chemical') :: ['thymidine'] @entity4540 :: ('MESH:D015774', 'Chemical') :: ['ganciclovir']
@entity3 :: (MESH:D009362,Disease) :: ['pleural metastasis']
