Lymph node @entity3 is a significant factor in @entity356 prognosis. It is well known that @entity5 cells secrete lymphangiogenic factors, thereby promoting lymphangiogenesis. However, the effects of lymphatic endothelial cell (LEC)-secreted factors on the process of lymphangiogenesis and @entity5 cell @entity3 remain unclear. We established an animal model and successfully isolated LECs from afferent lymph vessels of sentinel lymph nodes (SLNs) in animal models. A microarray analysis was performed to characterize gene expression profile in afferent LECs induced by @entity5 cells. There were significant differences in 846 genes between normal LECs and LECs with lymph node @entity3 . Among these genes, we found that expression of @entity2851 was upregulated, which was confirmed by quantitative reverse-transcriptase polymerase chain reaction. In a coculture system, @entity356 cells induced @entity2851 secretion from LECs, which was associated with the NF-kB pathway. @entity2851 stimulated LECs migration and tube formation involving FAK-ERK1/2- @entity3547 activation and reorganization of F-actin. In @entity1 @entity356 specimens, @entity7416 expression was positively correlated with @entity2802 ( @entity5 , node, @entity3 ) stage and lymphatic vessel density. These results suggested that LECs of afferent SLNs had specific expression profiles, which were distinct from those of normal lymphatic vessels and appeared to promote @entity3 . The expression pattern described in our study, including @entity2851 in LECs and its receptor @entity7416 in @entity5 cells, offers a promising therapeutic target for @entity356 .

Lymphatic endothelial cell-secreted @entity2851 stimulates lymphangiogenesis and XXXX of @entity356 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'Tumor', 'metastatic cancer'] @entity3547 :: ('387', 'Gene') :: ['RhoA'] @entity2851 :: ('2919', 'Gene') :: ['CXCL1'] @entity2802 :: ('10178', 'Gene') :: ['TNM'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity7416 :: ('3579', 'Gene') :: ['CXCR2'] @entity356 :: ('MESH:D013274', 'Disease') :: ['gastric cancer']
@entity3 :: (MESH:D009362,Disease) :: ['metastasis']
