The sequence of the @entity1792 synthase gene (gltA) of 13 ehrlichial species (Ehrlichia chaffeensis, @entity4209 , Ehrlichia muris, an Ehrlichia species recently detected from Ixodes ovatus, @entity38695 , @entity4210 , @entity4210 , the @entity1 @entity8116 [ @entity4210 , @entity16451 , @entity67768 , @entity42427 , @entity30953 , and @entity42427 helminthoeca) have been determined by degenerate PCR and the Genome Walker method. The ehrlichial gltA genes are 1,197 bp ( @entity42427 and @entity30953 ) to 1,254 bp (A. marginale and A. @entity67768 ) long, and GC contents of the gene vary from 30.5% (Ehrlichia sp. detected from I. ovatus) to 51.0% (A. @entity67768 ). The percent identities of the gltA nucleotide sequences among ehrlichial species were 49.7% ( @entity30953 versus A. @entity67768 ) to 99.8% ( @entity4210 versus @entity4210 ). The percent identities of deduced @entity535 sequences were 44.4% ( @entity42427 versus E. muris) to 99.5% ( @entity4210 versus @entity4210 ), whereas the homology range of 16S rRNA genes was 83.5% ( @entity30953 versus the Ehrlichia sp. detected from I. ovatus) to 99.9% ( @entity4210 , @entity4210 , and @entity4210 ). The architecture of the phylogenetic trees constructed by gltA @entity377 sequences or @entity535 sequences was similar to that derived from the 16S rRNA gene sequences but showed more-significant bootstrap values. Based upon the alignment analysis of the ehrlichial gltA sequences, two sets of primers were designed to amplify tick-borne Ehrlichia and @entity42427 genogroup Ehrlichia (N. helminthoeca, @entity42427 , and @entity30953 ), respectively. Tick-borne Ehrlichia species were specifically identified by restriction fragment length polymorphism (RFLP) patterns of AcsI and XhoI with the exception of E. muris and the very closely related ehrlichia derived from I. ovatus for which sequence analysis of the PCR product is needed. Similarly, @entity42427 genogroup Ehrlichia species were specifically identified by RFLP patterns of RcaI digestion. If confirmed this technique will be useful in rapidly identifying Ehrlichia spp.

XXXX synthase gene sequence: a new tool for phylogenetic analysis and identification of Ehrlichia.

@entity1 :: ('9606', 'Species') :: ['human'] @entity4209 :: ('944', 'Species') :: ['Ehrlichia canis'] @entity1792 :: ('MESH:D019343', 'Chemical') :: ['citrate'] @entity8116 :: ('MESH:D007960', 'Disease') :: ['granulocytic ehrlichiosis'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity377 :: ('CHEBI:36976', 'Chemical') :: ['nucleotide'] @entity30953 :: ('950', 'Species') :: ['Ehrlichia risticii', 'E. risticii'] @entity42427 :: ('951', 'Species') :: ['Neorickettsia', 'Ehrlichia sennetsu', 'E. sennetsu'] @entity16451 :: ('770', 'Species') :: ['Anaplasma marginale'] @entity67768 :: ('769', 'Species') :: ['Anaplasma centrale', 'centrale'] @entity38695 :: ('779', 'Species') :: ['Cowdria ruminantium'] @entity4210 :: ('948', 'Species') :: ['Ehrlichia phagocytophila', 'Ehrlichia equi', 'E. phagocytophila', 'E. equi', 'HGE agent', 'HGE] agent']
@entity1792 :: (MESH:D019343,Chemical) :: ['Citrate']
