OBJECTIVE: To assess the usefulness of enzyme-linked immunosorbent assay (ELISA) assessment of the combination of @entity50816 ( @entity50816 ) and @entity5858 in the diagnosis of bullous pemphigoid (BP). DESIGN: Retrospective study of serum samples from @entity1 with BP. SETTING: Tertiary care center. @entity1 : A total of 190 @entity1 with newly diagnosed BP and 78 controls with other @entity792 . INTERVENTION: Serum samples were tested using commercialized @entity50816 and @entity5858 ELISA and indirect immunofluorescence (IIF). MAIN OUTCOME MEASURES: The sensitivity and specificity of ELISA for the combination of @entity50816 and @entity5858 in the diagnosis of BP were contrasted with ELISA for each of the antigens alone and with IIF. RESULTS: The sensitivity and specificity of ELISA for the combination of @entity50816 and @entity5858 were 87% and 88%, respectively, compared with 79% and 90% for @entity5858 ELISA, 61% and 96% for @entity50816 ELISA, and 81% and 63% for IIF. The combination of @entity50816 ELISA and @entity5858 ELISA permitted 8% and 16% gains in sensitivity compared with each of @entity5858 ELISA and @entity50816 ELISA alone, respectively. Anti- @entity50816 antibodies were detected in 15 of 40 BP serum samples with no anti- @entity5858 antibodies (38%) and in 8 of 13 serum samples from @entity1 with BP and @entity33 (62%) compared with 2 of 22 samples of cicatricial pemphigoid (P = .002) and 0 of 16 epidermolysis bullosa @entity2653 serum samples (P < .001). The @entity5858 ELISA values were more closely correlated with initial extent of @entity4530 (r = 0.44, P < .001) than @entity50816 ELISA values (r = 0.16, P = .03). CONCLUSION: Since the combination of @entity50816 and @entity5858 ELISA only slightly increases the sensitivity of BP diagnosis over @entity5858 ELISA alone, @entity50816 ELISA could be adequately proposed in a minority of BP cases with @entity33 and in those with no circulating anti- @entity5858 antibodies.

Enzyme-linked immunosorbent assay for the combination of XXXX in the diagnosis of bullous pemphigoid.

@entity1 :: ('9606', 'Species') :: ['patients', 'PATIENTS'] @entity2653 :: ('MESH:C538175', 'Disease') :: ['acquisita'] @entity5858 :: ('1308', 'Gene') :: ['BPAG2'] @entity4530 :: ('MESH:D001768', 'Disease') :: ['BP lesions'] @entity33 :: ('MESH:D052016', 'Disease') :: ['mucosal involvement'] @entity50816 :: ('667', 'Gene') :: ['bullous pemphigoid antigen 1', 'BPAG1'] @entity792 :: ('MESH:D001327', 'Disease') :: ['autoimmune bullous diseases']
@entity50816 :: (667,Gene) :: ['bullous pemphigoid antigens 1 and 2']
