AIMS: Pancreatic b-cells synthesize and release @entity750 (5 @entity750 , @entity750 ); however, the role of @entity750 receptors on @entity413 stimulated insulin secretion (GSIS) and the mechanisms mediating this function is not fully understood. The aims of this study were to determine the expression profile of @entity750 receptors in @entity19 MIN6 b-cells and to examine the effects of pharmacological activation of @entity750 receptor @entity68865 on GSIS and mitochondrial function. MATERIALS AND METHODS: mRNA levels of @entity750 receptors in MIN6 cells were quantified by RT qPCR. GSIS was assessed in MIN6 cells in response to global serotonergic activation with @entity750 and pharmacological @entity68865 activation or inhibition with BW723C86 or @entity21484 , respectively. In response to @entity68865 activation also was evaluated the mRNA and protein levels of @entity7111 and PPARy by RT-qPCR and western blotting and mitochondrial function by @entity26 consumption rate (OCR) and @entity855 cellular content. RESULTS: We found that mRNA levels of most @entity750 receptors were either very low or undetectable in MIN6 cells. By contrast, @entity68865 mRNA was present at moderate levels in these cells. Preincubation (6 h) of MIN6 cells with @entity750 or BW723C86 reduced GSIS and the effect of @entity750 was prevented by @entity21484 . Preincubation with BW723C86 increased @entity7111 and PPARy mRNA and protein levels and decreased mitochondrial respiration and @entity855 content in MIN6 cells. CONCLUSIONS: Our results indicate that prolonged @entity68865 activation in @entity19 b-cells decreases @entity413 -stimulated insulin secretion and mitochondrial activity by mechanisms likely dependent on enhanced @entity7111 /PPARy expression.

Prolonged Activation of the @entity68865 Serotonin Receptor Impairs XXXX Stimulated Insulin Secretion and Mitochondrial Function in MIN6 Cells.

@entity19 :: ('10090', 'Species') :: ['murine'] @entity68865 :: ('15559(Tax:10090)', 'Gene') :: ['Htr2b'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity750 :: ('MESH:D012701', 'Chemical') :: ['5HT', 'serotonin', 'hydroxytryptamine'] @entity21484 :: ('MESH:C092756', 'Chemical') :: ['SB204741'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP'] @entity7111 :: ('19017(Tax:10090)', 'Gene') :: ['PGC1a']
@entity413 :: (MESH:D005947,Chemical) :: ['Glucose']
