OBJECTIVE: To evaluate the biocompatibility and osteoinductive properties of Bioroot    RCS (BR, Septodont, France) compared to Kerr's Pulp Canal Sealer    (PCS, Kerr, Italy) using the @entity19 pulp-derived stem cell line A4, which have an osteo/odontogenic potential in vitro and contribute to efficient bone repair in vivo. METHODS: A4 cells were cultured at the stem cell stage in the presence of solid disks of BR or PCS, whereas untreated A4 cells were used as control. After 3, 7, 10 days of direct contact with the sealers, cell viability was quantified using @entity3460 exclusion assay. Immunolabelings were performed to assess the expression of odontoblast markers i.e. type 1 collagen, @entity12281 or BSP. Finally, sealer-treated cells were induced toward osteo/odontogenic differentiation to assess the impact of the sealers on mineralization by Von Kossa staining. Statistical significance was evaluated by one-way analysis of variance and t-test (p<0.05). RESULTS: BR did not alter the viability and morphology of A4 pulpal cells compared to control group (p>0.05); however, living cell percentage of PCS was significantly lower compared to control and BR groups (p<0.05). BR preserved the intrinsic ability of A4 cells to express type 1 collagen, @entity12281 or BSP at the stem cell stage. It did not alter the integrity of collagen fibers surrounding the cells and promoted overexpression of BSP and @entity12281 at the cell surface. In contrast to PCS, BR did not compromise the mineralization potential of pulpal A4 stem cells. SIGNIFICANCE: Bioroot    RCS was not as cytotoxic as PCS. It did not recruit the pulpal stem cells toward differentiation but preserve their osteo-odontogenic intrinsic properties. Bioroot    RCS might provide more suitable environment to induce stem cells for hard tissue deposition.

In vitro bioactivity of Bioroot    RCS, via A4 XXXX pulpal stem cells.

@entity19 :: ('10090', 'Species') :: ['mouse'] @entity12281 :: ('13406(Tax:10090)', 'Gene') :: ['DMP1'] @entity3460 :: ('MESH:D014343', 'Chemical') :: ['Trypan Blue']
@entity19 :: (10090,Species) :: ['mouse']
