UNASSIGNED: The role of endocannabinoid system in @entity320 development and progression is actually not fully understood. This study was aimed at clarifying whether cannabinoid-type 1 ( @entity6510 ) receptor may function as @entity5 -promoting or -suppressing signal in @entity1 @entity320 . @entity6510 receptor expression was measured in @entity1 @entity320 cell lines by real-time PCR. A genetic deletion of @entity6510 receptors in selected @entity320 cells was carried out by using three different short hairpin RNAs (shRNAs). Performance of target gene silencing was verified by real-time PCR and Western blot. The effects of @entity6510 receptor silencing on cell growth, clonogenicity, migration capability, cell cycle progression, and activation of mitogenic signals was tested. Lentiviral shRNAs vectors targeting different regions of the @entity1 @entity6510 gene led to a significant reduction in @entity6510 receptor mRNA and a near complete loss of @entity6510 receptor protein, compared to control vector (LV-c). The number of viable cells, the colony-forming ability and cell migration were significantly reduced in cells transduced with @entity6510 lentiviral shRNAs compared to LV-c. Cell cycle analyses showed arrest at G1/S phase. p-Akt and @entity7952 expression were decreased in transduced versus control cells. Findings of this study suggest that @entity6510 receptor might function as @entity5 -promoting signal in @entity1 @entity320 .

Tumor-promoting effects of cannabinoid receptor type 1 in XXXX @entity320 cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity6510 :: ('1268', 'Gene') :: ['CB1'] @entity7952 :: ('9451', 'Gene') :: ['p-ERK'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma', 'cutaneous melanoma']
@entity1 :: (9606,Species) :: ['human']
