Glycation occurs in vivo as a result of the non-enzymatic reaction of @entity3363 (and/or their autoxidation products) with proteins, DNA or lipids. Protein glycation causes loss-of-function and consequently, the development of @entity6 . Glycation also boosts @entity6960 , which can be directly related with the higher prevalence of aggregating diseases in @entity6 @entity1 . However, the molecular mechanism connecting glycation with aggregation still remains unclear. Previously we described mechanistically how glycation of hen egg-white lysozyme (HEWL) with @entity8690 induced its aggregation. Here we address the question whether the @entity8690 -induced aggregation is a general process or it depends on the chemical nature of the glycating agent. Glycation of HEWL with glycolaldehyde occurs through two different scenarios depending on the HEWL concentration regime (both within the  M range). At low HEWL concentration non-crosslinking fluorescent advanced glycation end-products (AGEs) are formed on Lys side chains, which do not change the protein structure but inhibit its enzymatic activity. These AGEs have a little impact on HEWL surface hydrophobicity and therefore, a negligible effect on its aggregation propensity. Upon increasing HEWL concentration, the glycation mechanism shift towards the formation of intermolecular crosslinks, which trigger a polymerization cascade involving the formation of @entity1423 . These results notably differ with the aggregation-modulation mechanism of ribosylated HEWL directed by hydrophobic interactions. Additionally, their comparison constitutes the first experimental evidence showing that the mechanism underlying the aggregation of a glycated protein depends on the chemical nature of the glycating agent.

Glycation of lysozyme by glycolaldehyde provides new mechanistic insights in @entity6 -related XXXX .

@entity1 :: ('9606', 'Species') :: ['people'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetic', 'diabetic-related diseases'] @entity1423 :: ('MESH:D001791', 'Disease') :: ['insoluble spherical-like aggregates'] @entity8690 :: ('MESH:D012266', 'Chemical') :: ['ribose'] @entity6960 :: ('MESH:D001796', 'Disease') :: ['protein aggregation'] @entity3363 :: ('MESH:D002241', 'Chemical') :: ['carbohydrates']
@entity6960 :: (MESH:D001796,Disease) :: ['protein aggregation']
