OBJECTIVES: Our aim was to identify patterns in differentially regulated proteins associated with the progression of @entity712 . We specifically studied proteomics in chronic reversibly (RDM) and irreversibly dysfunctional myocardium (IRDM), as well as end-stage failing myocardium (ESFM). METHODS: We studied biopsies from 9 @entity1 with stable @entity712 undergoing @entity453 ( @entity453 ) (EF 34% +/- 3%) and from 4 @entity1 with ESFM undergoing heart transplantation (EF 17% +/- 5%). In @entity453 @entity1 paired echocardiographic studies before and 6 months after revascularization classified dysfunctional myocardium as RDM or IRDM. Regions with preserved contractile function served as control. We used two-dimensional gel electrophoresis (2D-PAGE) and computerized image analysis to investigate myocardial protein expression. Proteins were identified by in-gel digestion and subsequent liquid chromatography-tandem mass spectrometry (LC- @entity15 / @entity15 ). RESULTS: Among 3 significantly altered protein spots in RDM we identified 2 up-regulated glycolytic enzymes. In IRDM 15 proteins were signficantly altered of which we identified 10, among these 6 were down-regulated mitochondrial enzymes. In ESFM 9 of 12 significantly altered protein spots were identified. Six were down-regulated mitochondrial enzymes. CONCLUSION: Myocardial metabolism may be involved in the progression of @entity712 to irreversible dysfunction and end-stage @entity712 .

Proteomic analysis identifies mitochondrial metabolic enzymes as major discriminators between different stages of the failing XXXX myocardium.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity453 :: ('MESH:D003324', 'Disease') :: ['CABG', 'coronary artery bypass surgery'] @entity712 :: ('MESH:D006333', 'Disease') :: ['chronic heart failure', 'heart failure'] @entity15 :: ('MESH:D009103', 'Disease') :: ['MS']
@entity1 :: (9606,Species) :: ['human']
