UNASSIGNED: @entity238 Sarcomere Length Short ( @entity68821 ) is a recently identified @entity17454 protein homology 2 (WH2) domain protein involved in skeletal muscle thin filament regulation. @entity68821 was shown to be important for the establishment of the proper length and organization of sarcomeric actin filaments. Here we present the first detailed characterization of the biochemical activities of the tandem WH2 domains of @entity68821 ( @entity68821 ). Our results revealed that @entity68821 binds both monomeric and filamentous actin and shifts the monomer : filament equilibrium towards monomeric actin. In addition, @entity68821 can bind to but fails to depolymerize @entity6286 -, or @entity13298 -bound actin filaments. These interactions endow @entity68821 with two major activities in the regulation of actin dynamics: @entity68821 sequesters actin monomers into non-polymerizable complexes and enhances actin filament disassembly by severing, which is modulated by tropomyosin. We also show that @entity59931 does not influence the activities of the WH2 domains of @entity68821 in actin dynamics. In conclusion, the tandem WH2 domains of @entity68821 are multifunctional regulators of actin dynamics. Our findings suggest that the activities of the WH2 domains do not reconstitute the presumed biological function of the full-length protein. Consequently, the interactions of the WH2 domains of @entity68821 with actin must be tuned in the cellular context by other modules of the protein and/or sarcomeric components for its proper functioning.

Biochemical Activities of the XXXX Homology Region 2 Domains of Sarcomere Length Short.

@entity68821 :: ('41377(Tax:7227)', 'Gene') :: ['SALS', 'SALS-WH2'] @entity59931 :: ('33834(Tax:7227)', 'Gene') :: ['profilin'] @entity17454 :: ('MESH:D014923', 'Disease') :: ['Wiskott - Aldrich syndrome'] @entity13298 :: ('MESH:C057531', 'Chemical') :: ['jasplakinolide'] @entity6286 :: ('MESH:D010590', 'Chemical') :: ['phalloidin'] @entity238 :: ('7227', 'Species') :: ['Drosophila melanogaster']
@entity17454 :: (MESH:D014923,Disease) :: ['Wiskott-Aldrich Syndrome']
