A cell-by-cell analysis of the magnocellular elements in hypothalami of fifty Long-Evans (normal) and Brattleboro (diabetes insipidus) @entity35 was done using the unlabeled antibody enzyme technique (PAP) with primary antisera directed against @entity398 (OXY), @entity1951 ( @entity6085 ), and the neurophysins. The magnocellular neurons of the hypothalamus were found in the supraoptic ( @entity4525 ), paraventricular (PVN), and anterior commissural ( @entity8605 ) nuclei, a number of accessory nuclei, and as individual cells in the anterior @entity1640 area. @entity4525 was divided by the optic tract into the principal part and retrochiasmatic @entity4525 . In retrochiasmatic @entity4525 a majority of the cells contained vasopressin. Within the principal part of @entity4525 @entity398 -producing cells tended to be found rostrally and dorsally, while the vasopressin cells were more common caudally and ventrally. PVN was divided into three subnuclei, the medial, lateral, and posterior subnuclei, on the basis of cellular morphology and peptide content. The magnocellular cells of the medial and lateral PVN were closely packed together and nearly round, while those of posterior PVN were more separated and fusiform in shape with their long axis running in a medio-lateral direction. Medial PVN consisted primarily of @entity398 -producing cells, while lateral PVN was formed by a core of vasopressin-producing cells with a rim of @entity398 cells. Posterior PVN contained largely @entity398 -producing cells. Both @entity6085 and OXY cells were found in the accessory nuclei. In the Long-Evans @entity35 the @entity4525 had, on the average, 1443 OXY and 3236 @entity6085 cells; the PVN had 1174 OXY and 976 @entity6085 cells; and the accessory magnocellular groups in the hypothalamus (including the @entity8605 ) had 1286 OXY and 552 @entity6085 cells. The Brattleboro strain animal had similar numbers of cells in these nuclei. (The cells which contain @entity6085 in normal animals were identified in the Brattleboro @entity35 as large, neurophysin-negative cells.) Thus, a large fraction of the magnocellular @entity398 - and vasopressin-producing cells in the @entity35 were located outside of the PVN and @entity4525 . One accessory cell group in particular, @entity8605 , had 616 OXY cells, or about 50% as many as PVN. In each nucleus the sum of the numbers of OXY and @entity6085 cells was approximately the number of neurophysin cells.

Immunohistochemical analysis of magnocellular elements in @entity35 hypothalamus: distribution and numbers of cells containing neurophysin, XXXX , and vasopressin.

@entity398 :: ('MESH:D010121', 'Chemical') :: ['oxytocin'] @entity1640 :: ('MESH:D007027', 'Disease') :: ['hypothalamic'] @entity1951 :: ('MESH:D014667', 'Chemical') :: ['vasopressin'] @entity4525 :: ('304092(Tax:10116)', 'Gene') :: ['SON'] @entity8605 :: ('MESH:D016518', 'Disease') :: ['ACN'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity6085 :: ('MESH:D007177', 'Disease') :: ['ADH']
@entity398 :: (MESH:D010121,Chemical) :: ['oxytocin']
