Progenitor cells in the subventricular zone of the lateral ventricle and in the dentate gyrus of the hippocampus can proliferate throughout the life of the animal. To examine the proliferation and fate of progenitor cells in the subventricular zone and dentate gyrus after focal @entity2806 , we measured the temporal and spatial profiles of proliferation of cells and the phenotypic fate of proliferating cells in @entity63 brain in a model of @entity2500 in the adult @entity35 . Proliferating cells were labeled by injection of @entity1603 ( @entity1603 ) in a pulse or a cumulative protocol. To determine the temporal profile of proliferating cells, @entity63 @entity35 were injected with @entity1603 every 4 h for 12 h on the day preceding @entity204 . @entity35 were killed 2-14 days after @entity63 . We observed significant increases in numbers of proliferating cells in the ipsilateral cortex and subventricular zone 2-14 days with a peak at 7 days after @entity63 compared with the control group. To maximize labeling of proliferating cells, a single daily injection of @entity1603 was administered over a 14-day period starting the day after @entity63 . @entity35 were killed either 2 h or 28 days after the last injection of @entity1603 . A significant increase in numbers of @entity1603 immunoreactive cells in the subventricular zone was coincident with a significant increase in numbers of @entity1603 immunoreactive cells in the olfactory bulb 14 days after @entity63 and numbers of @entity1603 immunoreactive cells did not significantly increase in the dentate gyrus. However, 28 days after the last labeling, the number of @entity1603 labeled cells decreased by 90% compared with number at 14 days. Clusters of @entity1603 labeled cells were present in the cortex distal to the @entity1188 . Numerous cells immunostained for the polysialylated form of the neuronal cell adhesion molecule were detected in the ipsilateral subventricular zone. Only 6% of @entity1603 labeled cells exhibited glial fibrillary acidic protein immunoreactivity in the cortex and subcortex and no @entity1603 labeled cells expressed neuronal protein markers (neural nuclear protein and @entity19744 ). From these data we suggest that focal @entity2806 induces transient and regional specific increases in cell proliferation in the ipsilateral hemisphere and that proliferating progenitor cells may exist in the adult cortex.

Proliferation and differentiation of progenitor cells in the cortex and the subventricular zone in the adult XXXX after focal @entity2806 .

@entity1603 :: ('MESH:D001973', 'Chemical') :: ['BrdU', 'bromodeoxyuridine'] @entity2806 :: ('MESH:D002545', 'Disease') :: ['cerebral ischemia'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemia', 'ischemic'] @entity2500 :: ('MESH:D020244', 'Disease') :: ['embolic middle cerebral artery occlusion'] @entity1188 :: ('MESH:D007238', 'Disease') :: ['infarction'] @entity35 :: ('10116', 'Species') :: ['rat', 'Rats', 'rats'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death'] @entity19744 :: ('25595(Tax:10116)', 'Gene') :: ['microtubule associated protein-2']
@entity35 :: (10116,Species) :: ['rat']
