The phosphorylation status of cellular proteins results from an equilibrium between the activities of protein kinases and protein phosphatases (PPases). Reversible protein phosphorylation is an important aspect of signal transduction that regulate many biological processes in eukaryotic cells. The @entity375 genome encodes 40 PPases, including seven members of the protein phosphatase 2C subfamily ( @entity9792 to @entity65053 ). In contrast to other PPases, the cellular roles of PTCs have not been investigated in detail. Here, we sought to determine the cellular role of @entity65055 in @entity375 with disruption of PTC genes. We found that cells with   @entity65055 disruption were tolerant to the cell wall-damaging agents Congo red (CR) and calcofluor white (CFW); however, cells with simultaneous disruption of @entity9792 and @entity65055 were very sensitive to these agents. Thus, simultaneous disruption of @entity9792 and @entity65055 gave a synergistic response to cell wall damaging agents. The level of phosphorylated @entity19624 increased significantly after CR treatment in   @entity9792 cells and more so in   @entity9792  @entity65055 cells; therefore, deletion of @entity65055 enhanced @entity19624 phosphorylation in the   @entity9792 disruptant. The level of transcription of @entity68420 upon exposure to CR increased to a greater extent in the   @entity9792  @entity65055 double disruptant than the   @entity9792 single disruptant. The   @entity9792  @entity65055 double disruptant cells showed normal vacuole formation under standard growth conditions, but fragmented vacuoles were present in the presence of CR or CFW. Our analyses indicate that @entity375 @entity65055 participates in the negative regulation of @entity19624 phosphorylation and vacuole morphogenesis under cell wall stress conditions.

Type 2C protein phosphatase XXXX participates in activation of the @entity19624 -mediated cell wall integrity pathway in @entity375 .

@entity68420 :: ('853696(Tax:4932)', 'Gene') :: ['KDX1'] @entity65055 :: ('850441(Tax:4932)', 'Gene') :: ['ptc6', 'PTC6'] @entity65053 :: ('856475(Tax:4932)', 'Gene') :: ['PTC7'] @entity9792 :: ('851558(Tax:4932)', 'Gene') :: ['PTC1', 'ptc1'] @entity375 :: ('4932', 'Species') :: ['S.  cerevisiae', 'Saccharomyces cerevisiae'] @entity19624 :: ('856425(Tax:4932)', 'Gene') :: ['Slt2']
@entity65055 :: (850441(Tax:4932),Gene) :: ['Ptc6']
