UNASSIGNED: The present study aimed to investigate whether premature rupture of the fetal membrane, combined with subclinical @entity5870 , affects pregnancy outcomes. In addition, the association between premature rupture of the fetal membrane ( @entity4942 ) and the levels of @entity1476 ( @entity1476 ), an inactive proenzyme that can be activated by other factors or signals in @entity1 , was examined. In total, 80 pregnant @entity1 with @entity4942 were classified into the experimental and control groups, according to their final placental pathological diagnosis results. The 40 @entity1 in the experimental group suffered from subclinical @entity5870 , while the 40 @entity1 in the control group exhibited no lesions of the placenta or fetal membrane. Tissue samples were collected and the total protein and mRNA were extracted for western blot and quantitative polymerase chain reaction analyses. ELISA was performed in order to detect the levels of @entity1476 in the serum of the two groups of @entity1 . The rates of cesarean section, @entity13424 , postpartum @entity548 , preterm incidence, @entity8052 , residual placental blood and stillbirth were all significantly higher in the experimental group compared with the control group. In addition, the mRNA and protein expression levels of @entity1476 were reduced in the experimental group compared with the control group. ELISA results indicated that the serum @entity1476 concentrations were also reduced in the @entity1 with @entity4942 . Therefore, the present study demonstrated that the @entity4942 , combined with subclinical @entity5870 , significantly affected pregnancy outcomes and was associated with reduced levels of @entity1476 .

Premature rupture of the fetal membrane combined with subclinical @entity5870 negatively affects pregnancy outcomes by a mechanism associated with reduced levels of XXXX .

@entity1 :: ('9606', 'Species') :: ['humans', 'patients'] @entity548 :: ('MESH:D006470', 'Disease') :: ['hemorrhage'] @entity1476 :: ('4313', 'Gene') :: ['matrix metalloproteinase-2', 'MMP-2'] @entity5870 :: ('MESH:D002821', 'Disease') :: ['chorioamnionitis'] @entity13424 :: ('MESH:D011645', 'Disease') :: ['puerperal infection'] @entity8052 :: ('MESH:D010921', 'Disease') :: ['placenta accreta'] @entity4942 :: ('MESH:D005322', 'Disease') :: ['PROM']
@entity1476 :: (4313,Gene) :: ['matrix metalloproteinase-2']
