Orexin is a hypothalamic neuropeptide that regulates feeding behavior in @entity35 . @entity70037 has recently been cloned in @entity1707 and was shown to stimulate food intake after intramuscular injection. This study was designed to determine whether intracerebroventricular (ICV) and intravenous injections of orexin could regulate appetite in @entity742 . Suffolk wethers were moved to indoor facilities, adapted to diets for 6 wk, and trained to stand in stanchions for 3 to 6 h each day for 2 wk before indwelling ICV cannulas were installed. These @entity742 were provided water and they consumed feed ad libitum. On the day before an experiment, each @entity742 was cannulated in a jugular vein. On the day of an experiment, @entity742 were placed in stanchions and allowed to stand for 1 h before use. @entity742 were then monitored over a 2-h control period before i.v. injection with saline or porcine @entity70037 (3 micrograms/kg BW) or ICV injection with artificial cerebrospinal fluid (CSF), orexin (0.03, 0.3, or 3 micrograms/kg BW) or in a second experiment with either orexin B (0.03, 0.3, 3 micrograms/kg BW), @entity38025 ( @entity38025 ; 0.3 microgram/kg BW), or orexin plus @entity38025 . Food intake was monitored for consecutive 2-h periods. The i.v. injections of orexin did not affect food intake or metabolite or hormone concentrations. In ICV @entity742 , orexin increased food intake at 2 (P < 0.04) and at 4 h (P < 0.02). Food intake was greatest with the 0.3 microgram/kg BW dosage of orexin (P < 0.05). In the first 2 h after injection, orexin had an effect similar to that of @entity38025 (0.23 kg for orexin and 0.2 kg for @entity38025 ). The combination of @entity38025 and orexin had a greater effect on food intake (to 0.34 kg) than did either orexin (P < 0.05) or @entity38025 (P < 0.008) alone. Differences were not apparent in the subsequent 2-h interval. No differences were noted in free @entity844 , @entity413 , @entity12550 , luteinizing hormone, or insulin concentrations following orexin injection. There was an effect of ICV orexin treatment on plasma @entity701 concentrations (P < 0.002). @entity701 was increased by orexin at the 0- to 2-h (P < 0.008) and in the 2- to 4-h (P < 0.009) intervals after orexin injection. These data indicate that central administration of orexin stimulates feed intake in @entity742 .

Effect of intracerebroventricular @entity70037 on food intake in XXXX .

@entity1707 :: ('9823', 'Species') :: ['pigs'] @entity844 :: ('CHEBI:35366', 'Chemical') :: ['fatty acid'] @entity742 :: ('9940', 'Species') :: ['sheep', 'Sheep'] @entity12550 :: ('443329(Tax:9940)', 'Gene') :: ['growth hormone'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity701 :: ('MESH:D006854', 'Chemical') :: ['Cortisol', 'cortisol'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity38025 :: ('443508(Tax:9940)', 'Gene') :: ['NPY', 'neuropeptide-Y'] @entity70037 :: ('397305(Tax:9823)', 'Gene') :: ['orexin-B', 'Orexin-B']
@entity742 :: (9940,Species) :: ['sheep']
