Using the Evans Blue procedure, we previously found strain-related differences in plasma volumes in 5 inbred @entity35 strains. Because albumin binds strongly with Evans blue, this protein is important in the Evans blue method of plasma volume determination. Therefore, we speculated that interstrain differences in plasma albumin concentration (PAC) could distort calculated plasma volumes. To address this concern, we used ELISA techniques to measure PAC in these inbred @entity35 strains. In study A, the blood volume was measured by using Evans blue dye, and albumin was measured at the start of @entity548 . In study B, blood volume was not measured, and albumin was measured twice, near the start and end of @entity548 (approximately 14 min apart). Neither study revealed any interstrain differences in PAC, which decreased after @entity548 in all 5 strains. No correlation was found between PAC and plasma volume, survival time, blood @entity880 , or blood base excess. Percentage changes in PAC during @entity548 were greater in salt-sensitive compared with Lewis @entity35 . Moreover, these percentage changes were associated with survival time in Fawn hooded @entity101 @entity35 . Our data show that the plasma volumes we measured previously were not misrepresented due to variations in PAC.

Relationship between Plasma Albumin Concentration and Plasma Volume in 5 Inbred XXXX Strains.

@entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity101 :: ('MESH:D006973', 'Disease') :: ['hypertensive'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity548 :: ('MESH:D006470', 'Disease') :: ['hemorrhage']
@entity35 :: (10116,Species) :: ['Rat']
