Changes in membrane lipid composition play important roles in plant adaptation to and survival after freezing. Plant response to cold and freezing involves three distinct phases: cold acclimation, freezing, and post-freezing recovery. Considerable progress has been made toward understanding lipid changes during cold acclimation and freezing, but little is known about lipid alteration during post-freezing recovery. We previously showed that @entity68644 ( @entity68644 ) is involved in lipid hydrolysis and @entity958 freezing tolerance. This study was undertaken to determine how lipid species change during post-freezing recovery and to determine the effect of two PLDs, @entity68645 , on lipid changes during post-freezing recovery. During post-freezing recovery, hydrolysis of plastidic lipids, @entity10675 and plastidic @entity3634 , is the most prominent change. In contrast, during freezing, hydrolysis of extraplastidic phospholipids, @entity2434 and @entity3633 , occurs. Suppression of @entity30847 decreased phospholipid hydrolysis and @entity8558 production in both the freezing and post-freezing phases, whereas ablation of PLDdelta increased lipid hydrolysis and @entity8558 production during post-freezing recovery. Thus, distinctly different changes in lipid hydrolysis occur in freezing and post-freezing recovery. The presence of @entity30847 correlates with phospholipid hydrolysis in both freezing and post-freezing phases, whereas the presence of PLDdelta correlates with reduced lipid hydrolysis during post-freezing recovery. These data suggest a negative role for @entity30847 and a positive role for PLDdelta in freezing tolerance.

Differential degradation of extraplastidic and plastidic lipids during freezing and post-freezing recovery in XXXX .

@entity68644 :: ('829733(Tax:3702)', 'Gene') :: ['PLD', 'phospholipase D'] @entity958 :: ('3702', 'Species') :: ['Arabidopsis thaliana'] @entity68645 :: ('820816;829733', 'Gene') :: ['PLDalpha1 and PLDdelta'] @entity2434 :: ('MESH:D010713', 'Chemical') :: ['phosphatidylcholine'] @entity8558 :: ('MESH:D010712', 'Chemical') :: ['phosphatidic acid'] @entity30847 :: ('820816(Tax:3702)', 'Gene') :: ['PLDalpha1'] @entity3633 :: ('MESH:C483858', 'Chemical') :: ['phosphatidylethanolamine'] @entity3634 :: ('MESH:D010715', 'Chemical') :: ['phosphatidylglycerol'] @entity10675 :: ('MESH:C009909', 'Chemical') :: ['monogalactosyldiacylglycerol']
@entity958 :: (3702,Species) :: ['Arabidopsis thaliana']
