UNASSIGNED: @entity1460 consumption is increasing in many Western diets; however, recent reviews suggest that the prevalence of soy @entity566 can be as high as 0.5% for the general population and up to 13% for @entity1 . The immunoglobulin-E (IgE) binding of sera from six soy-sensitive adult @entity1 subjects to @entity1460 proteins separated by 2D gel electrophoresis was studied. Synthetic peptide sets spanning the mature glycinin subunit A2 and A3 primary sequences were used to map the IgE-binding regions. Putative epitopes identified in this study were also localized on glycinin hexamer models using bioinformatics software. We identified linear IgE-binding epitopes of the major storage protein Gly m 6 by screening individual soy-sensitive @entity1 sera. These epitopes were then further analysed by 3D in silico model localization and compared to other plant storage protein epitopes. Web-based software applications were also used to study the ability to accurately predict epitopes with mixed results. A total of nine putative IgE-binding epitopes were identified in the glycinin A3 (A3.1-A3.3) and A2 (A2.1-A2.6) subunits. Most @entity1 ' sera IgE bound to only one or two epitopes, except for one patient's serum which bound to four different @entity30844 . Two epitopes (A3.2 and A2.4) overlapped with a previously identified epitope hot spot of 11S globulins from other plant species. Most epitopes were predicted to be exposed on the surface of the 3D model of the glycinin hexamer. @entity3689 sequence alignments of @entity1460 acidic glycinins and other plant globulins revealed one dominant epitope hot spot among the four reported hot spots. This study may be helpful for future development of soy @entity566 immunotherapy and diagnosis.

Using patient serum to epitope map XXXX glycinins reveals common epitopes shared with many legumes and tree nuts.

@entity1 :: ('9606', 'Species') :: ['children', 'human', 'patients', 'patient'] @entity566 :: ('MESH:D006967', 'Disease') :: ['allergy'] @entity30844 :: ('MESH:C537089', 'Disease') :: ['A2 epitopes'] @entity1460 :: ('3847', 'Species') :: ['Soybean', 'soybean'] @entity3689 :: ('D000596', 'Chemical') :: ['Amino acid']
@entity1460 :: (3847,Species) :: ['soybean']
