UNASSIGNED: Small caliber synthetic vascular grafts are commonly used for bypass surgery and dialysis access sites but have high failure rates because of @entity8233 formation and @entity715 . Seeding synthetic grafts with endothelial cells (ECs) provides a biocompatible surface that may prevent graft failure. However, @entity1713 following exposure to blood flow still remains a major obstacle in the development of biosynthetic grafts. We tested the hypothesis that induced expression by the seeded EC, of vascular endothelial growth factor165 (VEGF165) and of @entity32879 , an extracellular matrix glycoprotein that has a crucial role in elastin fiber organization and increase EC adherence to surfaces, may improve long-term graft patency. Autologous ECs were isolated from venous segments, and were transduced with retroviral vectors expressing @entity32879 and VEGF165. The modified cells were seeded on expanded @entity736 (ePTFE) grafts and implanted in a large animal model. Three months after transplantation, all grafts seeded with modified EC were patent on a selective angiography, whereas only a third of the control grafts were patent. Similar results were shown at 6 months. Thus, seeding ePTFE vascular grafts with genetically modified EC improved long-term small caliber graft patency. The biosynthetic grafts may provide a novel therapeutic modality for @entity1 with @entity1095 and @entity1 requiring vascular access for hemodialysis.Gene Therapy advance online publication, 24 December 2015; doi:10.1038/gt.2015.104.

Co-expression of XXXX and VEGF165 increases long-term patency of synthetic vascular grafts seeded with autologous endothelial cells.

@entity736 :: ('CHEBI:53251', 'Chemical') :: ['polytetrafluoroethylene'] @entity1 :: ('9606', 'Species') :: ['patients'] @entity1095 :: ('MESH:D016491', 'Disease') :: ['peripheral vascular disease'] @entity1713 :: ('MESH:D012163', 'Disease') :: ['EC detachment'] @entity32879 :: ('10516', 'Gene') :: ['fibulin-5'] @entity715 :: ('MESH:D013927', 'Disease') :: ['thrombosis'] @entity8233 :: ('MESH:D058426', 'Disease') :: ['neointima']
@entity32879 :: (10516,Gene) :: ['fibulin-5']
