UNASSIGNED: Environmental temperature modulates @entity6145 (TH)-dependent metamorphosis in some amphibian species. The North American bullfrog - Rana (Lithobates) catesbeiana - tadpole is naturally adapted to a wide range of temperatures over multiple seasons. Cold temperatures delay while warmer temperatures accelerate metamorphosis. Exogenous TH exposure of premetamorphic tadpoles results in a rapid precocious induction of metamorphosis at warm temperatures (20-25  C). The same exposure at cold temperatures (4-5  C) does not elicit an overt metamorphic response. However, a molecular memory of TH exposure is established such that cold, TH-exposed tadpoles returned to permissive warm temperatures will rapidly execute TH-induced genetic programs. Previous mRNA profiling has identified TH-regulated transcription factors encoded by thra, thrb, thibz, @entity55615 , and @entity69008 as components of the molecular memory after one week post-exposure. However, a further hierarchy may exist within the initiation phase since many gene transcripts demonstrated tissue-specific patterns. Whether the molecular memory is organ autonomous or requires additional modulating factors is unknown. Herein we examine tail fin and back skin and determine that thibz is the only transcript that is TH-responsive after 2 days post-exposure at low temperature in both tissues in the intact animal. In back skin, @entity69008 is also TH-responsive under these conditions. Serum-free tail fin organ culture (C-Fin) reveals that the thibz response is organ autonomous whereas cultured back skin (C-Skin) results suggest that thibz and @entity69008 require an additional factor for induction from elsewhere within the intact animal. Subsequent investigations are now possible to identify endogenous factors that modulate the molecular memory in intact animals.

Identification of organ-autonomous constituents of the molecular memory conferred by XXXX exposure in cold temperature-arrested metamorphosing Rana (Lithobates) catesbeiana tadpoles.

@entity6145 :: ('CHEBI:60311', 'Chemical') :: ['thyroid hormone'] @entity55615 :: ('687', 'Gene') :: ['klf9'] @entity69008 :: ('51191', 'Gene') :: ['cebp1']
@entity6145 :: (CHEBI:60311,Chemical) :: ['thyroid hormone']
