UNASSIGNED: The presence of interval sentinel lymph nodes in @entity320 is documented in several studies, but controversies still exist about the management of these lymph nodes. In this study, an immunohistochemical evaluation of @entity5 cell proliferation and neo-angiogenesis has been performed with the aim of establishing a correlation between these two parameters between positive and negative interval sentinel lymph nodes. This retrospective study reviewed data of 23 @entity1 diagnosed with @entity320 . Bioptic specimens of interval sentinel lymph node were retrieved, and immunohistochemical reactions on tissue sections were performed using Ki67 as a marker of proliferation and @entity2279 as a blood vessel marker for the study of angiogenesis. The entire stained tissue sections for each case were digitized using Aperio Scanscope Cs whole-slide scanning platform and stored as high-resolution images. Image analysis was carried out on three selected fields of equal area using IHC Nuclear and Microvessel analysis algorithms to determine positive Ki67 nuclei and vessel number. @entity1 were divided into positive and negative interval sentinel lymph node groups, and the positive interval sentinel lymph node group was further divided into interval positive with @entity3944 and interval positive with macrometastasis subgroups. The analysis revealed a significant difference between positive and negative interval sentinel lymph nodes in the percentage of Ki67-positive nuclei and mean vessel number suggestive of an increased cellular proliferation and angiogenesis in positive interval sentinel lymph nodes. Further analysis in the interval positive lymph node group showed a significant difference between micro- and macrometastasis subgroups in the percentage of Ki67-positive nuclei and mean vessel number. Percentage of Ki67-positive nuclei was increased in the macrometastasis subgroup, while mean vessel number was increased in the @entity3944 subgroup. The results of this study suggest that the correlation between @entity5 cell proliferation and neo-angiogenesis in interval sentinel lymph nodes in @entity320 could be used as a good predictive marker to distinguish interval positive sentinel lymph nodes with @entity3944 from interval positive lymph nodes with macrometastasis subgroups.

Interval sentinel lymph nodes in XXXX : a digital pathology analysis of Ki67 expression and microvascular density.

@entity1 :: ('9606', 'Species') :: ['Patients', 'patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity2279 :: ('5175', 'Gene') :: ['CD31'] @entity3944 :: ('MESH:D061206', 'Disease') :: ['micrometastasis'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma']
@entity320 :: (MESH:D008545,Disease) :: ['melanoma']
