UNASSIGNED: In recent years, microRNA has become a hotspot in research on diseases, especially in the initiation and progression of different types of @entity5 . In this study, we found that miR-218 could inhibit growth and metabolism in @entity11 by directly targeting @entity37565 . First, we obtained data from the Chinese @entity11 (CGGA) database to analyze miR-218 expression in different grades of @entity11 . The effects of miR-218 on cell cycle progression and cell proliferation in U87 and U251 cell lines were investigated by flow cytometry, specifically CCK8 assay and tablet cloning, respectively. @entity413 consumption and @entity880 production of @entity11 cell lines were measured by correlative test kits. Furthermore, we used Western blot analysis and luciferase reporter assay to identify the direct and functional target of miR-218. Data from the CGGA database and real-time quantitative reverse transcription-PCR demonstrated that miR-218 was obviously reduced in @entity1 @entity463 tissues, as well as in the cell lines. When miR-218 level was elevated in vitro, cell cycle progression was arrested in the G1 phase, and cell proliferation was dramatically inhibited. Both @entity413 consumption and @entity880 production of @entity11 cells were significantly reduced. Western blot analysis and luciferase reporter assay revealed that @entity37565 was a direct target of miR-218 in @entity11 cells. This investigation demonstrated that elevated @entity37565 expression could partly weaken the effect of miR-218 in vitro. This study also showed that miR-218 may be a repressor in @entity11 by directly targeting @entity37565 , as well as a potential therapeutic target in @entity11 .

MiR-218 Inhibited Growth and Metabolism of XXXX @entity463 Cells by Directly Targeting @entity37565 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity11 :: ('MESH:D005910', 'Disease') :: ['Glioma Genome Atlas', 'glioma', 'gliomas'] @entity37565 :: ('1870', 'Gene') :: ['E2F2'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity463 :: ('MESH:D005909', 'Disease') :: ['glioblastoma'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose', 'Glucose']
@entity1 :: (9606,Species) :: ['Human']
