BACKGROUND: Mesenchymal stem cells (MSC) are multipotent progenitor cells characterized by their ability to both self-renew and differentiate into tissues of mesodermal origin. The plasticity or transdifferentiation potential of MSC is not limited to mesodermal derivatives, since under appropriate cell culture conditions and stimulation by bioactive factors, MSC have also been differentiated into endodermal (hepatocytes) and neuroectodermal (neurons) cells. The potential of MSC for hepatogenic and neurogenic differentiation has been well documented in different animal models; however, few reports are currently available on large animal models. In the present study we sought to characterize the hepatogenic and neurogenic differentiation and multipotent potential of @entity291 MSC (bMSC) isolated from bone marrow (BM) of abattoir-derived fetuses. RESULTS: Plastic-adherent bMSC isolated from fetal BM maintained a fibroblast-like morphology under monolayer culture conditions. Flow cytometric analysis demonstrated that bMSC populations were positive for MSC markers CD29 and CD73 and pluripotency markers @entity18820 and @entity27189 ; whereas, were negative for hematopoietic markers @entity59136 and @entity33854 . Levels of mRNA of hepatic genes a-fetoprotein (AFP), @entity32771 ( @entity32771 ), @entity68772 ( @entity68772 ), @entity68773 ( @entity68773 ), @entity669 aminotransferase (TAT) and cytochrome P450 (CYP3A4) were up-regulated in bMSC during a 28-Day period of hepatogenic differentiation. Functional analyses in differentiated bMSC cultures evidenced an increase (P < 0.05) in @entity32771 and @entity1133 production and glycogen storage. bMSC cultured under neurogenic conditions expressed NESTIN and @entity61583 proteins at 24 h of culture; whereas, at 144 h also expressed @entity68774 and PrPC. Levels of @entity61583 and @entity68774 mRNA were up-regulated at the end of the differentiation period. Conversely, bMSC expressed lower levels of @entity27189 mRNA during both hepatogenic and neurogenic differentiation processes. CONCLUSION: The expression patterns of linage-specific markers and the production of functional metabolites support the potential for hepatogenic and neurogenic differentiation of bMSC isolated from BM of abattoir-derived fetuses. The simplicity of isolation and the potential to differentiate into a wide variety of cell lineages lays the foundation for bMSC as an interesting alternative for investigation in MSC biology and eventual applications for regenerative therapy in veterinary medicine.

Hepatogenic and neurogenic differentiation of bone marrow mesenchymal stem cells from abattoir-derived XXXX fetuses.

@entity32771 :: ('280717(Tax:9913)', 'Gene') :: ['ALB', 'albumin'] @entity27189 :: ('538951(Tax:9913)', 'Gene') :: ['NANOG'] @entity59136 :: ('281051(Tax:9913)', 'Gene') :: ['CD34'] @entity1133 :: ('MESH:D014508', 'Chemical') :: ['urea'] @entity68774 :: ('353111(Tax:9913)', 'Gene') :: ['TRKA'] @entity68772 :: ('280699(Tax:9913)', 'Gene') :: ['alpha1 antitrypsin', 'a1AT'] @entity33854 :: ('407152(Tax:9913)', 'Gene') :: ['CD45'] @entity18820 :: ('282316(Tax:9913)', 'Gene') :: ['OCT4'] @entity68773 :: ('281194(Tax:9913)', 'Gene') :: ['CNX32', 'connexin 32'] @entity61583 :: ('281294(Tax:9913)', 'Gene') :: ['MAP2'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine'] @entity291 :: ('9913', 'Species') :: ['bovine']
@entity291 :: (9913,Species) :: ['bovine']
