The purpose of this study was to investigate the mechanism of effects of interferon-alpha ( @entity3027 ) on @entity1860 ( @entity1860 ). Bone marrow mononuclear cells (BMMNC) were obtained from heparinized blood of @entity1860 @entity1 by Ficoll-Paque density gradient centrifugation. The expressions of @entity4896 , @entity8730 , @entity2937 , HLA-ABC, HLA-DR and @entity2071 on DC induced by @entity3027 + @entity2841 , @entity3027 + @entity2841 + @entity3646 and @entity3646 + @entity2841 for 7 days in vitro were assayed by flow cytometry. The morphologic features were observed by transmission and optical microscopy. The mixed lymphocyte reactions (MLR) with DC were evaluated by @entity98 assay. The results showed that the DC cultured in different cytokine combinations expressed significantly higher levels of @entity4896 , HLA-ABC, HLA-DR, @entity2937 , @entity2071 , and @entity8730 than those in the precultured. The DC growing with @entity3027 + @entity2841 expressed significantly higher levels of HLA-ABC, HLA-DR than those in @entity2841 + @entity3646 . The @entity2937 expression and MLR levels in @entity3027 + @entity2841 + @entity3646 increased significantly. The expression rate of DC antigens and MLR in the IFN resistant group significantly lower than those in the newly diagnosed and the effectively treated groups after at least 6 months of @entity3027 treatment (P < 0.05). The DC from the IFN resistant group did not express significantly @entity2937 and MLR in @entity3027 + @entity2841 + @entity3646 groups compared to those in the newly diagnosed and IFN effective treated groups. It is concluded that the BMMNC from @entity1860 cultured in combination with @entity3027 and other cytokines can be induced into DC with typical morphologic and immunophenotypic characteristics. Addition of @entity3027 + @entity2841 + @entity3646 to DC cultures can significantly up-regulate the expression of major histocompatibility complex molecules, co-stimulatory molecules and various adhesion molecules. The deficiency of DC differentiation and function may play a role in the development of clinical resistance to @entity3027 .

[In vitro cytokines-induced differentiation in mononuclear cell derived dendritic cells from XXXX ].

@entity1 :: ('9606', 'Species') :: ['patients'] @entity2841 :: ('1437', 'Gene') :: ['GM-CSF'] @entity2937 :: ('942', 'Gene') :: ['CD86'] @entity8730 :: ('9308', 'Gene') :: ['CD83'] @entity3027 :: ('3439', 'Gene') :: ['IFN-alpha'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity2071 :: ('3383', 'Gene') :: ['CD54'] @entity3646 :: ('3565', 'Gene') :: ['IL-4'] @entity1860 :: ('MESH:D015464', 'Disease') :: ['CML', 'chronic myeloid leukemia'] @entity4896 :: ('909', 'Gene') :: ['CD1a']
@entity1860 :: (MESH:D015464,Disease) :: ['chronic myeloid leukemia']
