KEY MESSAGE: Identification and characterization of a semi-dwarfing gene ds-3 encoding a mutant DELLA protein regulating plant height through gibberellin signaling pathway. Lodging is one of the most important factors causing severe yield loss in @entity4335 . Utilization of semi-dwarf varieties has been proved the most effective way to increase lodging resistance and yield in many crops. To develop semi-dwarf germplasm in @entity4335 , we identified a semi-dwarf mutant ds-3 which showed a reduced response to phytohormones gibberellins (GAs). Genetic analysis indicated the dwarfism was controlled by a single semi-dominant gene, ds-3. The DS-3 gene was mapped to a genomic region on chromosome C07, which is syntenic to the region of a previously identified semi-dwarf gene ds-1 (BnaA06. @entity68909 ). In this region, DS-3 (BnaC07. @entity68909 ) gene was identified to encode a DELLA protein that functions as a repressor in GA signaling pathway. A substitution of @entity1521 to @entity368 was identified in ds-3 in the conserved VHYNP motif, which is essential for GA-dependent interaction between gibberellin receptor GID1 and DELLA proteins. Segregation analysis in the F2 population derived from the cross between ds-1 and ds-3 demonstrated that BnaA06. @entity68909 displayed a stronger effect on plant height than BnaC07. @entity68909 , indicating that different @entity68909 genes may play different roles in stem elongation. In addition to BnaA06. @entity68909 and BnaC07. @entity68909 , two more @entity68909 genes (BnaA09. @entity68909 and BnaC09. @entity68909 ) were identified in the @entity4335 ( @entity4335 ) genome. Reverse-transcription polymerase chain reaction (RT-PCR) and yeast two-hybrid (Y2H) assays suggest that both BnaA09. @entity68909 and BnaC09. @entity68909 are transcribed in leaves and stems and can mediate GA signaling in vivo. These genes represent potential targets for screening ideal semi-dwarfing alleles for @entity4335 breeding.

XXXX DS-3, encoding a DELLA protein, negatively regulates stem elongation through gibberellin signaling pathway.

@entity68909 :: ('106445425', 'Gene') :: ['RGA'] @entity1521 :: ('CHEBI:26271', 'Chemical') :: ['proline'] @entity4335 :: ('3708', 'Species') :: ['B. napus', 'Brassica napus', 'oilseed rape'] @entity368 :: ('CHEBI:25017', 'Chemical') :: ['leucine']
@entity4335 :: (3708,Species) :: ['Brassica napus']
