BACKGROUND: Previously published reports indicated that some enzymes of the central @entity505 metabolism (CCM), particularly those involved in glycolysis and the @entity6626 cycle, may contribute to regulation of DNA replication. However, vast majority of such works was performed with the use of @entity5 cells, in the light of @entity105 . On the other hand, recent experiments conducted on bacterial models provided evidence for the direct genetic link between CCM and DNA replication. Therefore, we asked if silencing of genes coding for glycolytic and/or Krebs cycle enzymes may affect the control of DNA replication in normal @entity1 fibroblasts. RESULTS: Particular genes coding for these enzymes were partially silenced with specific siRNAs. Such cells remained viable. We found that silencing of certain genes resulted in either less efficient or delayed enterance to the S phase. This concerned following genes: @entity26256 , @entity20678 , @entity49368 , @entity7501 , @entity19992 , @entity3343 , @entity7510 , @entity44010 , @entity6497 , @entity39806 , FH and @entity69794 . Decreased levels of expression of @entity26256 , GADPH, @entity7510 , @entity44010 , FH and @entity69794 caused also a substantial impairment in DNA synthesis efficiency. CONCLUSIONS: The presented results illustrate the complexity of the influence of genes coding for enzymes of glycolysis and the @entity6626 cycle on the control of DNA replication in @entity1 fibroblasts, and indicate which of them are especially important in this process.

Effects of partial silencing of genes coding for enzymes involved in glycolysis and XXXX cycle on the enterance of @entity1 fibroblasts to the S phase.

@entity1 :: ('9606', 'Species') :: ['human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity7501 :: ('2597', 'Gene') :: ['GAPDH'] @entity49368 :: ('7167', 'Gene') :: ['TPI'] @entity44010 :: ('50', 'Gene') :: ['ACO2'] @entity26256 :: ('3099', 'Gene') :: ['HK2'] @entity19992 :: ('2023', 'Gene') :: ['ENO1'] @entity20678 :: ('5213', 'Gene') :: ['PFKM'] @entity6626 :: ('CHEBI:27093', 'Chemical') :: ['tricarboxylic acid'] @entity69794 :: ('4191', 'Gene') :: ['MDH2'] @entity3343 :: ('3939', 'Gene') :: ['LDHA'] @entity505 :: ('MESH:D002244', 'Chemical') :: ['carbon'] @entity105 :: ('MESH:D063646', 'Disease') :: ['carcinogenesis'] @entity7510 :: ('1442', 'Gene') :: ['CS1'] @entity39806 :: ('6389', 'Gene') :: ['SDHA'] @entity6497 :: ('8801', 'Gene') :: ['SUCLG2']
@entity6626 :: (CHEBI:27093,Chemical) :: ['tricarboxylic acid']
