UNASSIGNED: Insect-borne plant viruses cause significant agricultural losses and jeopardize sustainable global food production. While blocking plant virus transmission would allow for crop protection, virus receptors in insect vectors are unknown. Here we identify membrane alanyl aminopeptidase N ( @entity10793 ) as a receptor for @entity4137 ) coat protein (CP) in the gut of the @entity6294 , @entity6294 using a far-western blot method. Pull-down and immunofluorescence binding assays, and surface plasmon resonance were used to confirm and characterize CP - @entity10793 interaction. @entity69592 virions and a peptide comprised of @entity69592 coat protein fused to GFP (CP-P-GFP) specifically bound to @entity10793 . Recombinant @entity10793 expressed in Sf9 cells resulted in internalization of CP-P-GFP, which was visualized by confocal microscopy; such internalization is an expected hallmark of a functional gut receptor. Finally, in assays with aphid gut-derived brush border membrane vesicles (BBMV), binding of CP-P-GFP competed with binding of GBP3.1, a peptide previously demonstrated to bind to @entity10793 in the aphid gut, and to impede @entity69592 uptake into the hemocoel; this finding supports the hypothesis that GBP3.1 and @entity69592 bind to and compete for the same @entity10793 receptor. These in vitro data combined with previously published in vivo experiments (S. Liu, S. Sivakumar, W.O. Sparks, W.A. Miller, B.C. Bonning, Virology 401:107-16, 2010, doi: 10.1016/j.virol.2010.02.009) support identification of @entity10793 as the first receptor in a plant virus vector. Knowledge of this receptor will provide for technologies based on @entity69592 - @entity10793 interaction designed to block plant virus transmission and to suppress aphid populations. IMPORTANCE: A significant proportion of global food production is lost to insect pests. Aphids, in addition to weakening plants by feeding on their sap, are responsible for transmitting about half of the plant viruses vectored by insects. Growers rely heavily on the application of chemical insecticides to manage both aphids and aphid-vectored plant @entity1800 . To increase our understanding of plant virus-aphid vector interaction, we provide in vitro evidence supporting earlier in vivo work for identification of a receptor protein in the aphid gut called aminopeptidase N, which is responsible for entry of the plant virus @entity4137 into the @entity6294 vector. Enrichment of proteins found on the surface of the aphid gut epithelium resulted in identification of this first aphid gut receptor for a plant virus. This discovery is particularly important as @entity778 binding to such a receptor may enable development of a non-chemical strategy for controlling aphid-vectored plant viruses to maximize food production.

In vitro Evidence Supports Membrane Alanyl Aminopeptidase N as a Receptor for a Plant Virus in the XXXX Vector.

@entity10793 :: ('290', 'Gene') :: ['APN'] @entity6294 :: ('7029', 'Species') :: ['Acyrthosiphon pisum', 'pea aphid'] @entity778 :: ('MESH:D015658', 'Disease') :: ['disruption of plant virus'] @entity1800 :: ('MESH:D014777', 'Disease') :: ['viral disease'] @entity4137 :: ('193121', 'Species') :: ['Pea enation mosaic virus', 'Pea enation mosaic virus (PEMV'] @entity69592 :: ('12209', 'Species') :: ['PEMV']
@entity6294 :: (7029,Species) :: ['Pea Aphid']
