UNASSIGNED: A distributed network of neurons regulates wake, non-rapid eye movement (NREM) sleep, and REM sleep. However, there are also glia in the brain, and there is growing evidence that neurons and astroglia communicate intimately to regulate behaviour. To identify the effect of optogenetic stimulation of astrocytes on sleep, the promoter for the astrocyte-specific cytoskeletal protein, @entity9327 ( @entity9327 ) was used to direct the expression of channelrhodopsin-2 (ChR2) and the linked reporter gene, enhanced yellow fluorescent protein (EYFP), in astrocytes. rAAV- @entity9327 -ChR2 ( @entity59440 )-EYFP or rAAV- @entity9327 -EYFP was microinjected (750  nL) into the posterior hypothalamus (bilateral) of @entity19 . Three weeks later baseline sleep was recorded (0  Hz) and 24  h later optogenetic stimulation applied during the first 6  h of the lights-off period. @entity19 with ChR2 were given 5, 10 or 30  Hz stimulation for 6  h (10-ms pulses; 1  mW; 1  min on 4  min off). At least 36  h elapsed between the stimulation periods (5, 10, 30  Hz) and although 0  Hz was always first, the order of the other three stimulation rates was randomised. In @entity19 with ChR2 (n  =  7), 10  Hz, but not 5 or 30  Hz stimulation increased both NREM and REM sleep during the 6-h period of stimulation. Delta power did not increase. In control @entity19 (no ChR2; n  =  5), 10  Hz stimulation had no effect. This study demonstrates that direct stimulation of astrocytes powerfully induces sleep during the active phase of the sleep-wake cycle and underlines the inclusion of astrocytes in network models of @entity25 .

Optogenetic stimulation of astrocytes in the posterior hypothalamus increases sleep at night in C57BL/6J XXXX .

@entity19 :: ('10090', 'Species') :: ['mice', 'Mice'] @entity59440 :: ('p|SUB|H|134|R', 'ProteinMutation') :: ['H134R'] @entity25 :: ('MESH:D012893', 'Disease') :: ['sleep-wake regulation'] @entity9327 :: ('14580(Tax:10090)', 'Gene') :: ['glial fibrillary acidic protein', 'GFAP']
@entity19 :: (10090,Species) :: ['mice']
