Adiponectin belongs to the group of biologically active substances secreted by adipocytes and referred to as adipokines. Disturbances in its secretion and/or action are thought to be involved in the pathogenesis of some @entity209 . However, regulation of adiponectin secretion is poorly elucidated. In the present study, short-term regulation of adiponectin secretion in primary @entity35 adipocytes was investigated. Isolated @entity35 adipocytes were incubated in Krebs-Ringer buffer containing 5 mM @entity413 and insulin alone or in the combination with @entity1425 , @entity8279 , @entity859 A(1) receptor antagonist ( @entity3743 ), @entity2365 , @entity17293 or inhibitor of mitochondrial electron transport ( @entity3788 ). Adipocyte exposure for 2 h to insulin (1-100 nM) significantly increased secretion of adiponectin compared with secretion observed without insulin. Furthermore, secretion of adiponectin from adipocytes incubated with @entity413 and insulin was reduced by 1 and 2 microM @entity1425 , but not by 0.25 and 0.5 microM @entity1425 . Under similar conditions, 1 and 2 mM @entity8279 substantially diminished secretion of adiponectin, whereas 0.5 mM @entity8279 was ineffective. Secretion of adiponectin was found to be effectively decreased by @entity3743 . Moreover, adipocyte exposure to @entity3788 also resulted in a substantial diminution of secretory response of adipocytes incubated for 2 h with @entity413 and insulin. It was also demonstrated that @entity2365 and @entity17293 (0.06-0.5 mM) failed to affect secretion of leptin. The obtained results indicated that in short-term regulation of adiponectin secretion, insulin and @entity1425 exert the opposite effects. These effects appeared as early as after 2 h of exposure. Moreover, deprivation of energy or blockade of @entity859 action substantially decreased secretion of adiponectin.

Short-term regulation of adiponectin secretion in XXXX adipocytes.

@entity859 :: ('MESH:D000241', 'Chemical') :: ['adenosine'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity2365 :: ('CHEBI:7896', 'Chemical') :: ['palmitate'] @entity1425 :: ('MESH:D004837', 'Chemical') :: ['epinephrine'] @entity3788 :: ('MESH:D012402', 'Chemical') :: ['rotenone'] @entity17293 :: ('MESH:C022776', 'Chemical') :: ['2-bromopalmitate'] @entity8279 :: ('CHEBI:50095', 'Chemical') :: ['dibutyryl-cAMP'] @entity209 :: ('MESH:D008659', 'Disease') :: ['metabolic diseases'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity3743 :: ('MESH:C051360', 'Chemical') :: ['DPCPX']
@entity35 :: (10116,Species) :: ['rat']
