UNASSIGNED: Gene alterations are a well-established mechanism leading to drug resistance in @entity3052 cells. A full understanding of the mechanisms of drug resistance in these cells will facilitate more effective chemotherapy. In this study, we investigated the mechanism(s) of drug resistance in the @entity1 @entity3052 cell line MOLT-3 and its @entity8779 -resistant derivative MOLT-3/IDR through complete mitochondrial and nuclear DNA analyses. We identified genetic differences between these two cell lines. The ND3 mutation site ( @entity52211 ) in the mitochondrial DNA sequence was unique to MOLT-3/IDR cells. Moreover, we identified five candidate genes harboring genetic alterations, including @entity36548 , via CGH array analysis. Sequencing of the @entity36548 exon revealed a @entity68995 mutation present within the stop codon in MOLT-3/IDR cells but absent from MOLT-3 cells. This mutation led to an additional 18 @entity3534 in the protein encoded by @entity36548 . Using real-time PCR, we determined an expression value for this gene of 0.35. Protein structure predictions confirmed a structural change in @entity36548 in MOLT-3/IDR cells that corresponded to the site of the mutation. We speculate that this mutation may be related to @entity8779 resistance.

Analysis to Estimate Genetic Variations in the XXXX -Resistant Derivative MOLT-3.

@entity1 :: ('9606', 'Species') :: ['human'] @entity3534 :: ('MESH:D000596', 'Chemical') :: ['amino acids'] @entity8779 :: ('MESH:D015255', 'Chemical') :: ['idarubicin'] @entity36548 :: ('2590', 'Gene') :: ['GALNT2'] @entity52211 :: ('p|SUB|T|61|I', 'ProteinMutation') :: ['p.Thr61Ile'] @entity3052 :: ('MESH:D015470', 'Disease') :: ['acute leukemia'] @entity68995 :: ('p|SUB|G|1716|K', 'ProteinMutation') :: ['G1716K']
@entity8779 :: (MESH:D015255,Chemical) :: ['Idarubicin']
