BACKGROUND/AIM: There is evidence of a possible etiological role of @entity1 papillomaviruses (HPVs) in the development of @entity5 . Loss of function of the wild-type @entity876 @entity5 suppressor gene product by binding to E6 oncoproteins of high-risk HPVs is considered an important event in @entity5 development. The aim of this study was to verify the prevalence of @entity281 and @entity876 mutation in @entity5 tissue samples and in the adjacent normal mucosa in @entity1 from a high-risk area in Italy. METHODS: DNA from 33 biopsy specimens (17 @entity5 sample biopsies and 16 samples of adjacent normal mucosa) was screened for HPV DNA using two polymerase chain reaction based procedures. Restriction fragment length polymorphism analysis was used for typing. Screening of @entity876 mutations was performed with polymerase chain reaction-single strand conformation polymorphism analysis and DNA sequencing. RESULTS: Overall, 8 of 17 @entity1 presented HPV DNA; @entity1799 was detected in 4 of 8 samples. Samples from @entity5 and adjacent mucosa were positive for mucosal HPVs in 7 of 17 and 4 of 16 cases, respectively. In 1 case, HPV DNA was detected in the normal mucosa only. None of the samples contained HPVs of the @entity8323 or cutaneous groups. Mutations of @entity876 were detected in two HPV DNA negative samples. In both cases, the mutation was present in the @entity5 only. CONCLUSIONS: Our results are in favor of the involvement of both aberrant @entity876 expression and @entity281 in the development of @entity5 . The high @entity281 rate in @entity1 from a high-risk region suggests that subjects harboring HPVs (in particular @entity1799 ) in the esophagus should be considered at risk of @entity5 .

Detection of human papillomavirus DNA and XXXX gene mutations in esophageal cancer samples and adjacent normal mucosa.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'esophageal tumor', 'tumors', 'esophageal malignancies', 'esophageal tumors'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity281 :: ('MESH:D007239', 'Disease') :: ['HPV infection'] @entity1799 :: ('333760', 'Species') :: ['HPV 16'] @entity8323 :: ('MESH:D004819', 'Disease') :: ['epidermodysplasia verruciformis']
@entity876 :: (7157,Gene) :: ['p53']
