OBJECTIVE: @entity2057 ( @entity2057 ) is an @entity14827 . It is characterized by selective loss of spinal cord motor neurons leading to @entity2057 and is the result of mutation or deletion of the survival motor neuron (SMN) gene. Currently, there are no effective therapies for this disease. Stem cell therapy is a new prospect for @entity2057 @entity1 . This study aimed to investigate whether mesenchymal stem cells (MSCs) can be differentiated into neuron-like cells (NLCs) in @entity2057 @entity1 in order to provide a basis for stem cell therapy for @entity2057 . METHODS: @entity2057 was definitively diagnosed using polymerase chain reaction-restriction fragment length polymerphhism (PCR-RFLP). Two @entity1 without @entity11280 gene deletion were used as controls. MSCs were isolated and purified from @entity2057 @entity1 and controls, and induced into NLCs by @entity540 and @entity7651 . The NLCs were identified by immunofluourescence staining with @entity3508 and NF monoclonal antibodies. RESULTS: @entity2057 @entity1 showed the deletion of @entity11280 exon 7. The morphous and proliferative speed of MSCs between @entity2057 @entity1 and controls were similar. After 6-day induction, MSCs of the two groups displayed similar morphology to that of neurons, with long processes forming extensive networks. @entity3508 and NF, the neuronal markers, were detected in the differentiated NLCs of the two groups. CONCLUSIONS: @entity11280 deletion appears not to affect the proliferation and differentiation of MSCs. MSCs of @entity2057 @entity1 can be differentiated into NLCs.

[An experimental research on differentiation of mesenchymal stem cells derived from XXXX with spinal muscular atrophy into neuron-like cells].

@entity1 :: ('9606', 'Species') :: ['children', 'patients'] @entity7651 :: ('MESH:C038044', 'Chemical') :: ['baicalin'] @entity540 :: ('2247', 'Gene') :: ['bFGF'] @entity3508 :: ('2026', 'Gene') :: ['NSE'] @entity11280 :: ('6606', 'Gene') :: ['SMN1'] @entity2057 :: ('MESH:D009133', 'Disease') :: ['Spinal muscular atrophy', 'muscle atrophy', 'SMA'] @entity14827 :: ('MESH:D020271', 'Disease') :: ['autosomal recessive neurodegenerative disease']
@entity1 :: (9606,Species) :: ['children']
