@entity3123 still has the highest mortality rate in comparison to any other @entity5 . Major reasons are late detection of disease, highly aggressive @entity5 growth and the early formation of @entity3 . Thus, novel effective therapies are urgently needed to improve the outcome of the @entity1 . Overexpression of the @entity470 ( @entity470 ) and its ligands has been implicated in the oncogenesis of @entity3123 and associated with an unfavorable prognosis. Consequently, the @entity470 represents a specific target antigen suitable for immunotherapy. We generated a recombinant immunotoxin by fusing the anti- @entity470 single chain fragment 425( @entity10390 ) to a truncated mutant of Pseudomonas Exotoxin A (ETA'). Using the expression vector pBM1.1, functional 425( @entity10390 )-ETA' was periplasmically expressed under osmotic stress conditions in the presence of compatible solutes. The 72 kDa His10-tagged fusion protein was purified by a combination of metal-ion affinity and molecular size chromatography. Binding activity and specificity of the immunotoxin to the @entity470 -positive @entity3123 cell line L3.6pl was confirmed by flow cytometry and ELISA. Finally, 425( @entity10390 )-ETA' showed significant @entity137 toward this cell line reaching 50% inhibition of cell proliferation at a concentration (IC50) of 7.5 ng/ml. This is the first report documenting the specific @entity137 of a recombinant immunotoxin towards metastatic @entity3123 cells, suggesting that @entity470 -specific antibody toxins may become valuable therapeutic reagents for the treatment of @entity3123 .

The recombinant anti-EGF receptor immunotoxin 425( @entity10390 )-ETA' suppresses growth of a highly metastatic XXXX cell line.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity3123 :: ('MESH:C562463', 'Disease') :: ['pancreatic carcinoma', 'Pancreatic carcinoma'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity', 'cytotoxicity'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'malignancy'] @entity470 :: ('1956', 'Gene') :: ['EGFR', 'epidermal growth factor receptor'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastases'] @entity10390 :: ('652070', 'Gene') :: ['scFv']
@entity3123 :: (MESH:C562463,Disease) :: ['pancreatic carcinoma']
