@entity601 are the most common @entity600 associated with @entity6 . Although the teratogenic effects of excess @entity413 have been investigated in in vivo and in vivo studies, a cellular basis for @entity601 has not been elucidated. We used @entity35 embryo culture to study the organogenesis period of development, with excess @entity413 added to the serum medium to induce @entity601 . Light and electron microscopic examination of control 12-day-old embryos grown 48 hours in culture revealed blastlike cells with few organelles or cellular processes. Twelve-day-old embryos cultured in excess @entity413 had advanced cellular maturation with differentiation, including the presence of free polysomes and copious cell processes, regardless of whether they had an open neural tube. Cytoarchitectural changes such as decreased numbers of mitotic figures with mitotic cells in the mantle layer were focally distributed throughout the neural epithelium but with predominance at the site of failed closure. In vivo studies failed to demonstrate neural processes in day 12 normal embryos. Fourteen-day-old embryos grown in utero also had foci of cell processes in the neural tube but to a much lesser degree than that observed in the in vitro day 12 @entity413 -exposed embryos. The cellular aberrations in the excess @entity413 -treated embryos are characteristic of a premature maturational change. Since they are present in excess @entity413 -exposed embryos with or without failure of neural tube closure, these maturational and cytoarchitectural changes may contribute to the cellular basis for @entity601 .

Ultrastructural analysis of XXXX induced by in vitro hyperglycemic conditions.

@entity6 :: ('MESH:D003920', 'Disease') :: ['diabetic pregnancies'] @entity600 :: ('MESH:D000014', 'Disease') :: ['malformations'] @entity601 :: ('MESH:D009436', 'Disease') :: ['Neural tube defects', 'neural tube anomalies', 'neural tube defects'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose', 'd-glucose']
@entity600 :: (MESH:D000014,Disease) :: ['malformations of the embryonic neural axis']
