BACKGROUND: Perls' stain is routinely used to demonstrate @entity68 in liver biopsies. We tested the hypothesis that it may be unnecessary in cases, where no @entity68 or another similar pigment was seen on the routine @entity1710 and @entity1711 ( @entity8108 and E) stained section. AIM: The aim of this study was to evaluate the efficiency of @entity8108 and E stain in demonstrating @entity68 in liver biopsies as well as to determine the possibility of replacing Perls' stain with @entity8108 and E stain. MATERIALS AND METHODS: Two hundred pairs of slides of liver biopsies were taken from the archival files of the Department of Pathology from 2006 to 2011. Perls' and @entity8108 and E slides were independently reviewed for the presence of @entity68 . RESULTS: Hundred and one cases showed the presence of @entity68 using @entity8108 and E stain. 84 of 86 cases showed positive @entity68 using both Perls' and @entity8108 and E stains. Seventeen cases were positive using @entity8108 and E stain but negative with Perls'. Only two cases did not show the presence of @entity68 using @entity8108 and E stain. Ninety-seven cases were negative using both Perls' and @entity8108 and E stains. @entity8108 and E stain showed a sensitivity, specificity, accuracy, positive predictive valve, and negative predictive value of 97.67%, 85.08%, 90.5%, 83.16%, and 97.98%, respectively. CONCLUSION: We demonstrate that the @entity8108 and E stain is a sensitive method to detect @entity68 pigment in liver biopsies, particularly when present in large quantities. A negative @entity8108 and E stain might obviate the need for extra Perls' staining, thus saving costs and shortening report turn-around times.

XXXX and @entity1711 stain shows a high sensitivity but sub-optimal specificity in demonstrating @entity68 pigment in liver biopsies.

@entity8108 :: ('D006859', 'Chemical') :: ['H'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hematoxylin'] @entity68 :: ('MESH:D007501', 'Chemical') :: ['iron']
@entity1710 :: (MESH:D006416,Chemical) :: ['Hematoxylin']
