PURPOSE: To generate @entity7944 ( @entity7944 ) from @entity19 induced pluripotent stem (iPS) cells. METHODS: Recently, a protocol for self-organizing optic cup morphogenesis in three-dimensional culture was reported, and it was suggested that ocular tissue derived from neural ectoderm could be differentiated. We demonstrated that a @entity7944 -like double-layered structure could be induced in simple culture by using a modified Eiraku differentiation protocol. RESULTS: Differentiation of a @entity7944 -like double-layered structure could be promoted by @entity6913 ( @entity6913 ) inhibitor. @entity16899 and @entity23070 were expressed in both thin layers, and induced @entity7944 -like cells expressed ciliary marker genes, such as @entity6834 , @entity68961 , @entity11579 , @entity54432 , @entity68962 , @entity5004 , @entity7739 , and @entity17736 . Increases in cytoplasmic and nuclear b-catenin in aggregates of the @entity7944 -like double-layered structure were confirmed by Western blot analysis. In addition, tankyrase inhibitor prevented the induction of the @entity7944 -like double-layered structure by @entity6913 inhibitor. Dye movement from pigmented cells to nonpigmented cells in the @entity19 iPS cell-derived @entity7944 -like structure was observed in a fluid movement experiment, consistent with the physiological function of @entity7944 in vivo. CONCLUSIONS: We could differentiate @entity7944 from @entity19 iPS cells in the present study. In the future, we hope that this @entity7944 -like complex will become useful as a graft for transplantation therapy in pathologic @entity551 due to @entity66 , and as a screening tool for the development of drugs for diseases associated with @entity7944 function.

Induction of Functional 3D Ciliary Epithelium-Like Structure From XXXX Induced Pluripotent Stem Cells.

@entity16899 :: ('14609(Tax:10090)', 'Gene') :: ['Connexin43'] @entity11579 :: ('21808(Tax:10090)', 'Gene') :: ['tgfb2'] @entity6913 :: ('56637(Tax:10090)', 'Gene') :: ['glycogen synthase kinase 3b', 'GSK-3b'] @entity68962 :: ('67866(Tax:10090)', 'Gene') :: ['wfdc1'] @entity66 :: ('MESH:D004194', 'Disease') :: ['CE dysfunction'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity6834 :: ('12444(Tax:10090)', 'Gene') :: ['cyclinD2'] @entity7944 :: ('MESH:C536309', 'Disease') :: ['CE', 'ciliary epithelium'] @entity5004 :: ('18423(Tax:10090)', 'Gene') :: ['otx1'] @entity23070 :: ('11826(Tax:10090)', 'Gene') :: ['aquaporin1'] @entity17736 :: ('12162(Tax:10090)', 'Gene') :: ['BMP7'] @entity551 :: ('MESH:D007022', 'Disease') :: ['ocular hypotension'] @entity68961 :: ('22771(Tax:10090)', 'Gene') :: ['zic1'] @entity54432 :: ('56847(Tax:10090)', 'Gene') :: ['aldh1a3'] @entity7739 :: ('12159(Tax:10090)', 'Gene') :: ['BMP4']
@entity19 :: (10090,Species) :: ['Mouse']
