UNASSIGNED: Understanding how @entity413 metabolism is finely regulated at molecular and cellular levels in the liver is critical for knowing its relationship to related pathologies, such as @entity6 . In order to gain insight the regulation of @entity413 metabolism, we studied the liver expressed isoforms aldolase B and @entity948 -1,6-bisphosphatase ( @entity24154 ), key enzymes in gluconeogenesis, analyzing their cellular localization in hepatocytes under different metabolic conditions and their protein-protein interaction in vitro and in vivo . We observed that @entity413 , @entity2690 , glucagon and adrenaline differentially modulate the intracellular distribution of aldolase B and @entity24154 . Interestingly, the in vitro protein-protein interaction analysis between aldolase B and @entity24154 showed a specific and regulatable interaction between them, whereas aldolase A (muscle isozyme) and @entity24154 showed no interaction. The affinity of the aldolase B and @entity24154 complex was modulated by intermediate metabolites, but only in the presence of @entity3529 . We observed a decreased association constant in the presence of @entity860 , @entity28235 , @entity16117 and inhibitory concentrations of @entity4567 . Conversely, the association constant of the complex increased in the presence of @entity12036 and non-inhibitory concentrations of @entity4567 . Notably, i n vivo fluorescence resonance energy transfer studies confirmed the interaction between aldolase B and @entity24154 . Also, the co-expression of aldolase B and @entity24154 in cultured cells suggested that @entity24154 guides the cellular localization of aldolase B. Our results provide further evidence that metabolic conditions modulate aldolase B and @entity24154 activity at the cellular level via the regulation of their interaction, suggesting that their association confers a catalytic advantage for both enzymes.

A new level of regulation in gluconeogenesis: Metabolic state modulates the intracellular localization of aldolase B and its interaction with liver XXXX -1,6-bisphosphatase.

@entity3529 :: ('D011188', 'Chemical') :: ['K(+)'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetes'] @entity948 :: ('MESH:D005632', 'Chemical') :: ['fructose'] @entity12036 :: ('MESH:D004099', 'Chemical') :: ['dihydroxyacetone phosphate'] @entity2690 :: ('3630', 'Gene') :: ['insulin'] @entity24154 :: ('2203', 'Gene') :: ['FBPase-1'] @entity16117 :: ('MESH:C027618', 'Chemical') :: ['fructose-6-phosphate'] @entity4567 :: ('MESH:C029063', 'Chemical') :: ['fructose-1,6-bisphosphate'] @entity28235 :: ('MESH:C027652', 'Chemical') :: ['fructose-2,6-bisphosphate'] @entity860 :: ('MESH:D000249', 'Chemical') :: ['adenosine monophosphate'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity948 :: (MESH:D005632,Chemical) :: ['fructose']
