BACKGROUND: @entity4373 ( @entity4373 ) regulation is extensive and shapes the functional complexity of higher organisms. However, the contribution of alternative splicing to fungal biology is not well studied. RESULTS: This study provides sequences of the transcriptomes of the plant wilt pathogen @entity23696 , using two different strains and multiple methods for cDNA library preparations. We identified alternatively spliced mRNA isoforms in over a half of the multi-exonic fungal genes. Over one-thousand isoforms involve TopHat novel splice junction; multiple types of combinatory alternative splicing patterns were identified. We showed that one Verticillium gene could use four different 5' splice sites and two different 3' donor sites to produce up to five mature mRNAs, representing one of the most sophisticated alternative splicing model in eukaryotes other than animals. Hundreds of novel intron types involving a pair of new splice sites were identified in the @entity23696 genome. All the types of @entity4373 events were validated by using RT-PCR. Functional enrichment analysis showed that @entity4373 genes are involved in most known biological functions and enriched in @entity855 biosynthesis, @entity458 , morphogenesis, signal transduction etc., predicting that the @entity4373 regulation modulates mRNA isoform output and shapes the @entity23696 proteome plasticity of the pathogen in response to the environmental and developmental changes. CONCLUSIONS: These findings demonstrate the comprehensive alternative splicing mechanisms in a fungal plant pathogen, which argues the importance of this fungus in developing complicate genome regulation strategies in eukaryotes.

Transcriptome analysis reveals the complexity of alternative splicing regulation in the fungus XXXX .

@entity4373 :: ('MESH:C536589', 'Disease') :: ['Alternative splicing', 'AS'] @entity458 :: ('MESH:D020018', 'Disease') :: ['sexual/asexual reproduction'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP'] @entity23696 :: ('27337', 'Species') :: ['Verticillium dahliae', 'V. dahliae']
@entity23696 :: (27337,Species) :: ['Verticillium dahliae']
