OBJECTIVE: Acids, such as those used in adhesive dentistry, have been shown to solubilize bioactive molecules from dentin. These @entity2483 ( @entity2483 ) may promote cell proliferation and differentiation, and ultimately contribute to dentin regeneration. The objective of this study was to evaluate the potential for varying concentrations of @entity2483 extracted from @entity1 dentin by @entity8718 of a range of pHs to stimulate proliferation and mineralization of two different cultured pulp cell populations. METHODS: @entity2483 were solubilized from powdered @entity1 dentin (7 days - 4  C) by @entity8718 of pH 1, 3, and 5 and also, @entity1098 . Extracts were dialyzed for 7 days against distilled water and lyophilized. Undifferentiated @entity19 dental pulp cells (OD-21) and cells of the odontoblast-like cell line (MDPC-23) were seeded in six-well plates (1*10(5)) and cultured for 24h in DMEM (Dulbecco's modified Eagle's medium) containing 10% (v/v) @entity2241 ( @entity2241 ). The cells were washed with serum-free medium and then treated with different concentrations of @entity2483 (0.01, 0.1, 1.0 and 10.0 g/ml) daily in serum free medium for 7 days. After 3, 5 (MDPC-23 only), and 7 days of treatment, cell proliferation was measured using 10vol% @entity30367 solution, which was added to each well for 1h. Cell numbers were first measured by cell counting ( @entity3460 ; n=5) and Alamar blue fluorescence to validate the assay, which was then used for the subsequent assessments of proliferation. Mineralization was assessed by @entity6033 assay after 12 days exposure to @entity2483 (n=5). Controls were media-only (DMEM) and @entity619 ( @entity9170 ; positive control). Results were analysed by ANOVA/Tukey's (p <=0.05). RESULTS: There was a linear correlation between cell counts and Alamar blue fluorescence (R(2)>0.96 for both cell types) , verifying the validity of the Alamar blue assay for these cell types. In general, there was a dose-dependent trend for enhanced cell proliferation with higher concentration of @entity2483 for both cell lines, especially at 10.0 g/ml. @entity9170 exposure resulted in significantly higher mineralization, but did not affect cell proliferation. @entity2483 exposure demonstrated significantly greater mineralization than media-only control for 10 g/ml for all extracts, and at lower concentrations for @entity1098 and pH 5 extracts. SIGNIFICANCE: @entity1 @entity2483 solubilized by acids at pH levels found in commercial dentin adhesives enhanced cell proliferation and mineralization of @entity19 and @entity35 undifferentiated dental pulp cells when presented in adequate concentration.

Dentin matrix components extracted with XXXX enhance cell proliferation and mineralization.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity8718 :: ('MESH:C030242', 'Chemical') :: ['phosphoric acid'] @entity9170 :: ('D003907', 'Chemical') :: ['DEX'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity30367 :: ('MESH:C005843', 'Chemical') :: ['Alamar blue'] @entity6033 :: ('MESH:C004468', 'Chemical') :: ['Alizarin Red S'] @entity619 :: ('MESH:D003907', 'Chemical') :: ['dexamethasone'] @entity3460 :: ('MESH:D014343', 'Chemical') :: ['Trypan blue'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity2483 :: ('MESH:C566443', 'Disease') :: ['DMC', 'dentin matrix components'] @entity1098 :: ('MESH:D004492', 'Chemical') :: ['EDTA'] @entity2241 :: ('MESH:D005315', 'Disease') :: ['FBS', 'fetal bovine serum']
@entity8718 :: (MESH:C030242,Chemical) :: ['phosphoric acid']
