UNASSIGNED: In the "liquidus tracking" (LT) approach to cryopreservation both the temperature and the concentration of cryoprotectant (CPA) are controlled such that solution composition "tracks" the liquidus (melting point) line for that system. Ice crystal formation is prevented but the tissue is not exposed to CPA concentrations exceeding those experienced by cells during conventional cryopreservation. This approach is particularly appropriate for articular cartilage because chondrocytes in situ are exquisitely susceptible to damage by the crystallisation of ice. This project aimed to develop a suitable process for tissue to be used in the surgical repair of damaged @entity1 knee joints. A high proportion of the chondrocytes should be alive. @entity1 articular cartilage was obtained from deceased donors and @entity3733 ( @entity3733 ) was used as the CPA, cooling was at 0.14  C/min and warming at 0.42  C/min. The vehicle solution was CPTes2. A program of increasing @entity3733 concentration was developed for cooling and this gave satisfactory tissue concentrations but reduction of @entity3733 concentration during warming was inadequate, resulting in higher tissue concentrations than required. Biomechanical testing indicated a compressive modulus of 9.5  1.3MPa in LT-processed cartilage, with control values of 11.6  0.8MPa (p>0.05, Student's t-test). Measurement of GAG synthesis sometimes approached 65% or 85% of control, but the variability of replicate data prevented firm conclusions. Ideally allograft tissue should score 1A or above on the Noyes scale and the donor age should be less than 46years but the cartilage used in this study did not meet these standards.

A liquidus tracking approach to the cryopreservation of XXXX cartilage allografts.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity3733 :: ('MESH:D004121', 'Chemical') :: ['dimethyl sulphoxide', 'DMSO']
@entity1 :: (9606,Species) :: ['human']
