BACKGROUND: @entity4115 is a common problem and, to date, there is no agent to preserve testicular function following detorsion. Platelet-rich plasma ( @entity16447 ), with its rich growth factor composition, has proven beneficial in regenerative therapy. It is believed that @entity16447 has not been studied in testis for @entity63 /reperfusion (I/R) injury. OBJECTIVE: This study investigated the effect of @entity16447 in an I/R @entity35 model 1 month after detorsion. STUDY DESIGN: Of 24 adult male @entity35 , 18 were randomly assigned into three groups, with six in each: control, I/R and I/R  +  @entity16447 . The @entity16447 was prepared from the remaining six. Each group underwent right orchiectomy. @entity63 was performed by rotating the left testis 720   and fixing with a nylon suture for 4  h. Reperfusion occurred 4  h later by removing the suture, and @entity16447 was administered at a dose of 10   l (2000  *  10(9)/l) into the left testis via the intraparenchymal route. Animals were sacrificed at the fourth week, and testes were taken for @entity1234 ( @entity1865 ), @entity2041 ( @entity6172 ), @entity224 dismutase (SOD), @entity1589 ( @entity1589 ), @entity1810 ( @entity1810 ), and @entity4124 measurements. RESULTS: @entity63 /reperfusion caused a significant increase in @entity1865 , @entity1589 and @entity4124 activity, and significant decrease in @entity6172 levels and SOD activity. The @entity16447 treatment helped correct the alterations in SOD, @entity4124 , and @entity1589 activities and @entity1865 levels. However, the mean @entity1865 level and @entity1589 activity were not totally restored compared with the controls. Serum @entity251 levels of the I/R group were significantly lower compared with the control and I/R  +  @entity16447 groups. @entity1810 and @entity4124 protein expressions were significantly higher in the I/R group compared with the control group and were low with @entity16447 administration compared with I/R groups (summary Table). DISCUSSION: The findings of the present study suggest that @entity16447 , by inhibiting neutrophil infiltration and oxidative stress and increasing antioxidant defense, exerts protective effects on testicular tissues against I/R. This study had some limitations: a scoring system was not used in the assessment of spermatogenesis in the histopathological findings and specific testis cell types were not histologically assessed. CONCLUSIONS: In light of the biochemical, histological and, especially, hormonal findings, intraparenchymal @entity16447 injection may have a protective effect in testicular tissue against I/R injury.

Effects of platelet-rich plasma against experimental @entity63 /reperfusion injury in XXXX testis.

@entity251 :: ('MESH:D013739', 'Chemical') :: ['testosterone'] @entity63 :: ('MESH:D007511', 'Disease') :: ['Ischemia', 'ischemia'] @entity16447 :: ('287750(Tax:10116)', 'Gene') :: ['PRP'] @entity4115 :: ('MESH:D013086', 'Disease') :: ['Testicular torsion'] @entity2041 :: ('MESH:D005978', 'Chemical') :: ['glutathione'] @entity4124 :: ('25402(Tax:10116)', 'Gene') :: ['caspase-3'] @entity6172 :: ('D005978', 'Chemical') :: ['GSH'] @entity1234 :: ('MESH:D008315', 'Chemical') :: ['malondialdehyde'] @entity1810 :: ('59086(Tax:10116)', 'Gene') :: ['transforming growth factor b', 'TGF-b'] @entity35 :: ('10116', 'Species') :: ['rat', 'Sprague-Dawley rats'] @entity1589 :: ('303413(Tax:10116)', 'Gene') :: ['MPO', 'myeloperoxidase'] @entity224 :: ('MESH:D013481', 'Chemical') :: ['superoxide'] @entity1865 :: ('CHEBI:566274', 'Chemical') :: ['MDA']
@entity35 :: (10116,Species) :: ['rat']
