PURPOSE: Acanthamoeba attachment (adsorption) to hydrogel contact lenses is enhanced by @entity796 biofilm. The effect of @entity11141 on Acanthamoeba attachment to biofilm-coated and uncoated hydrogel lenses was investigated. DESIGN: Experimental study. @entity1 AND CONTROLS: A minimum of 16 replicates were used for each test condition; a control condition using clean lenses without biofilm was included. METHODS: Four groups of hydrogel contact lenses (etafilcon A) were pretreated with @entity796 to form a biofilm. In addition, two more groups remained untreated. Quartered lenses of all six groups were then incubated in a suspension of A. castellanii trophozoites. Two batches of lenses had either 3 or 30 mM @entity11141 added to the bacterial suspension (stage 1 intervention). Two other batches of lenses had salicylate added to the amoebal suspension (stage 2 intervention). One of the batches, which had a stage 1 intervention, had salicylate added at the second stage as well. The remaining batches received no salicylate exposure and included lenses with and without biofilm coating. MAIN OUTCOME MEASURE: The outcome measure in this study was the number of Acanthamoeba trophozoites attached, per square centimeter, to the hydrogel surfaces. RESULTS: Biofilm coating from @entity796 gave a significantly increased attachment of A. castellanii trophozoites to the contact lens. When introduced at a first (biofilm) stage, second (trophozoite attachment) stage, or with intervention at both stages, 30 mM @entity11141 reduced amoebal attachment to the hydrogel lens. When applied to both stages and when applied at stage 2 to the biofilm coated contact lenses, 3 mM @entity11141 reduced amoebal attachment. The 3 mM concentration was not effective for the lower level of amoebae attachment to uncoated (nonbiofilm) lenses. CONCLUSIONS: @entity11141 successfully reduced amoebal trophozoite attachment to hydrogel lenses. This was the result of one of the following possibilities or a combination thereof: inhibition of biofilm formation; a direct effect on the amoebae; an alteration in the biofilm-amoebal attachment and resulting modification of the hydrogel lens surface. The results of this study suggest the major action is at stage 2 (on amoebal attachment to lenses) and favors alteration of the biofilm-amoebal attachment mechanism. This study demonstrates @entity2697 's potential benefit as a component of contact lens care solutions, designed to reduce microbial attachment and the risk of @entity281 .

@entity2697 inhibition of Acanthamoeba attachment to contact lenses: a model to reduce risk of XXXX .

@entity1 :: ('9606', 'Species') :: ['PARTICIPANTS'] @entity796 :: ('287', 'Species') :: ['Pseudomonas aeruginosa', 'P. aeruginosa'] @entity2697 :: ('CHEBI:30762', 'Chemical') :: ['salicylate'] @entity11141 :: ('MESH:D012980', 'Chemical') :: ['Sodium salicylate', 'sodium salicylate'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection']
@entity281 :: (MESH:D007239,Disease) :: ['infection']
