BACKGROUND: @entity9150 is generally regarded as a safe microorganism and is used to produce many biochemicals, including @entity8854 . @entity2518 ( @entity6053 ) is an @entity8854 derived non-protein @entity3689 , and is widely applied in fields such as medicine and agriculture. RESULTS: The products of the gltX, hemA, and hemL genes participate in the synthesis of @entity6053 from @entity8854 . Their annotated @entity9150 homologs were shown to be functional using heterologous complementation and overexpression techniques. Coexpression of hemA and hemL in native host led to the accumulation of @entity6053 , suggesting the potential of @entity9150 to produce @entity6053 for research and commercial purposes. To improve @entity6053 production, we constructed recombinant @entity9150 strains expressing hemA and hemL derived from different organisms. Transcriptome analysis indicated that the dissolved @entity26 level and @entity8798 concentration had major effects on @entity6053 synthesis. The downstream pathway of @entity1446 biosynthesis was inhibited using small molecules or introducing genetic modifications. Small-scale flask cultures of engineered @entity9150 produced 1.79  g/L of @entity6053 . CONCLUSION: Functional characterization of the key enzymes indicated complex regulation of the @entity1446 biosynthetic pathway in @entity9150 . Systematic analysis and molecular genetic engineering of @entity9150 may facilitate its development as a system for large-scale synthesis of @entity6053 .

Engineering @entity9150 to produce XXXX from glucose.

@entity9150 :: ('1718', 'Species') :: ['Corynebacterium glutamicum', 'C. glutamicum'] @entity8854 :: ('CHEBI:29985', 'Chemical') :: ['L-glutamate'] @entity8798 :: ('CHEBI:29033', 'Chemical') :: ['Fe(2+)'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity2518 :: ('CHEBI:17549', 'Chemical') :: ['5-Aminolevulinic acid'] @entity3689 :: ('D000596', 'Chemical') :: ['amino acid'] @entity6053 :: ('CHEBI:27432', 'Chemical') :: ['ALA'] @entity1446 :: ('MESH:D006418', 'Chemical') :: ['heme']
@entity2518 :: (CHEBI:17549,Chemical) :: ['5-aminolevulinic acid']
