The role of @entity8613 in the transduction of the hyperprolinaemic and hypertrehalosaemic signal of the endogenous neuropeptide Mem-CC was investigated in the cetoniid beetle Pachnoda sinuata. Flight and injection of Mem-CC into the haemocoel of the beetle induce an increase of @entity268 levels in the fat body of the beetle. This increase is tissue-specific and does not occur in brain and flight muscles. An elevation of @entity268 levels was also found when in vitro preparations of fat body tissue were subjected to Mem-CC. Elevation of the @entity268 concentration after injection of Mem-CC is time- and dose-dependent: the maximum response is measured after 1 min, and a dose of 25 pmol Mem-CC is needed. Injection of cpt- @entity268 , a @entity268 analogue which penetrates the cell membrane, causes a stimulation of @entity1521 synthesis but no mobilisation of @entity1735 reserves. The same is measured when @entity1922 , an inhibitor of phosphodiesterase, is injected. @entity6708 seems not to be involved in synthesis of @entity1521 nor @entity1735 release, because injection of cpt- @entity6708 has no influence on the levels of @entity1521 , @entity493 and @entity3363 in the haemolymph. Although glycogen phosphorylase of the fat body is activated by Mem-CC in a time- and dose-dependent manner, it cannot be stimulated by cpt- @entity268 . The combined data suggest that @entity268 is involved in regulation of @entity1521 levels by Mem-CC but not in regulation of @entity3363 . @entity11879 has no effect on metabolites in the haemolymph and is not capable of activating glycogen phosphorylase, indicating that it is not involved in the regulation of substrates in this beetle. Furthermore, the requirements of the receptor of Mem-CC are different for eliciting a hypertrehalosaemic and a hyperprolinaemic effect, respectively, suggesting that differentiation in signal transduction begins at the receptor level.

Cyclic AMP mediates the elevation of XXXX by AKH peptides in the cetoniid beetle, Pachnoda sinuata.

@entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity6708 :: ('CHEBI:16356', 'Chemical') :: ['cGMP'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['carbohydrate'] @entity1922 :: ('MESH:D015056', 'Chemical') :: ['IBMX'] @entity8613 :: ('MESH:D009712', 'Chemical') :: ['cyclic nucleotides'] @entity11879 :: ('MESH:D009655', 'Chemical') :: ['Octopamine'] @entity1521 :: ('CHEBI:26271', 'Chemical') :: ['proline'] @entity493 :: ('CHEBI:16449', 'Chemical') :: ['alanine'] @entity3363 :: ('MESH:D002241', 'Chemical') :: ['carbohydrates']
@entity1521 :: (CHEBI:26271,Chemical) :: ['proline']
