@entity375 ribosomal protein S14 (rpS14) binds to two different RNA molecules: (1). helix 23 of 18S rRNA during its assembly into 40S ribosomal subunits and (2). a stem-loop structure in @entity61751 pre-mRNA to repress expression of the @entity61751 gene. We used the three-dimensional structure of @entity4691 ribosomal protein S11, a bacterial homologue of rpS14, as a guide to identify conserved, surface-exposed @entity535 residues that are likely to contact RNA. Eight residues that met these criteria were mutated to @entity493 . Most of these mutations affected interaction of rpS14 with either helix 23 or the @entity61751 stem-loop RNA or both. Assembly of 40S ribosomal subunits and repression of @entity61751 were also affected. S11 contains an extended @entity1473 -terminal domain rich in basic @entity1522 , which interacts with rRNA. We systematically evaluated the importance of each of the last ten @entity535 residues in the basic, @entity1473 -terminal tail of @entity375 rpS14 for binding to RNA, by mutating each to @entity493 . Mutations in nine of these residues decreased binding of rpS14 to one or both of its RNA ligands. In addition, we examined the importance of four structural motifs in helix 23 of 18S rRNA for binding to rpS14. Mutations that altered either the terminal loop, the G-U base-pair closing the terminal loop, or the internal loop affected binding of rpS14 to helix 23.

Interactions of XXXX ribosomal protein rpS14 with RNA.

@entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity4691 :: ('274', 'Species') :: ['Thermus thermophilus'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity61751 :: ('853248(Tax:4932)', 'Gene') :: ['RPS14B'] @entity375 :: ('4932', 'Species') :: ['yeast', 'Yeast'] @entity493 :: ('CHEBI:16449', 'Chemical') :: ['alanine'] @entity1473 :: ('CHEBI:46883', 'Chemical') :: ['carboxy']
@entity375 :: (4932,Species) :: ['yeast']
