The @entity3903 gene, cellular homolog of the avian v- @entity4987 oncogene, encodes two alternatively spliced adaptor signaling proteins, @entity69670 (28 kDa) and @entity4987 (40 kDa). Both @entity69670 have been shown to activate kinase signaling and anchorage-independent growth in vitro and @entity69670 transformed cells readily form @entity5 in @entity19 . Affymetrix oligonucleotide arrays were used to analyse 86 lung @entity420 and 10 uninvolved lung tissues. @entity3903 mRNA expression was increased in more advanced (stage III versus stage I), larger (T(2-4) versus T(1)), and poorly differentiated @entity5 and in @entity5 from @entity1 demonstrating poor survival (P=0.00034). An overlapping series of 93 lung @entity420 (64 stage I and 29 stage III) and 10 uninvolved lung specimens were measured for quantitative differences in @entity69670 and @entity4987 protein levels using 2-D PAGE. @entity4987 protein spots were identified using mass spectrometry and 2-D Western blotting. A significant increase in levels of the @entity69670 oncoprotein and the phosphorylated isoform of @entity4987 was observed in @entity5 (P<0.05). No difference in protein level was evident between stages. Concordant with mRNA expression, @entity69670 and @entity4987 were increased in poorly differentiated @entity5 (P<0.05). @entity4987 immunohistochemical analysis of @entity5 tissue arrays using the same @entity5 series also demonstrated increased abundance of nuclear and cytoplasmic @entity4987 in more @entity5 (P<0.05). This study provides the first quantitative analysis of discrete @entity69670 and @entity4987 protein isoforms in @entity1 @entity2 and provides evidence that the @entity3903 proto-oncogene may foment a more @entity146 phenotype in @entity2 .

Increased XXXX proto-oncogene expression is associated with an @entity146 phenotype in lung @entity420 .

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity69670 :: ('12928(Tax:10090)', 'Gene') :: ['CRKI', 'CRKI and CRKII'] @entity146 :: ('MESH:D001523', 'Disease') :: ['aggressive'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'proliferative tumors', 'tumors'] @entity19 :: ('10090', 'Species') :: ['nude mice'] @entity4987 :: ('1398', 'Gene') :: ['CRKII', 'CRK', 'crk'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung tumors', 'lung cancers'] @entity420 :: ('MESH:D000230', 'Disease') :: ['adenocarcinomas'] @entity3903 :: ('23552', 'Gene') :: ['C-CRK']
@entity3903 :: (23552,Gene) :: ['C-CRK']
