Apart from control of circulating fluid, atrial natriuretic peptide (ANP) exhibits anti-inflammatory effects in the lung. However, molecular mechanisms of ANP anti-inflammatory effects are not well-understood. Peripheral @entity44753 ( @entity44753 ) dynamics is essential for agonist-induced regulation of vascular endothelial permeability. Here we studied the role of @entity44753 -dependent signaling in ANP protective effects against endothelial cell (EC) barrier dysfunction and @entity647 induced by @entity794 -derived peptidoglican-G (PepG). PepG-induced @entity455 was accompanied by @entity44753 destabilization and disruption of @entity44753 network. ANP attenuated PepG-induced @entity44753 disassembly, NFkB signaling and activity of @entity44753 -associated Rho activator @entity52905 leading to attenuation of EC inflammatory activation reflected by expression of adhesion molecules @entity8024 and @entity6580 . ANP-induced EC barrier preservation and @entity44753 stabilization were linked to phosphorylation and inactivation of @entity44753 -depolymerizing protein stathmin. Expression of stathmin phosphorylation-deficient mutant abolished ANP protective effects against PepG-induced @entity32 and EC permeability. In contrast, siRNA-mediated stathmin knockdown prevented PepG-induced @entity1897 disassembly and endothelial barrier dysfunction. ANP protective effects in a @entity19 model of PepG-induced @entity651 were associated with increased phosphorylation of stathmin, while exacerbated @entity651 in the ANP knockout @entity19 was accompanied by decreased pool of stable @entity44753 . Stathmin knockdown in vivo reversed exacerbation of @entity651 in the ANP knockout @entity19 . These results show a novel @entity44753 -mediated mechanism of endothelial barrier protection by ANP in pulmonary EC and animal model of PepG-induced @entity651 via stathmin-dependent control of @entity44753 assembly.

Role of microtubules in attenuation of PepG-induced XXXX by atrial natriuretic peptide.

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity455 :: ('MESH:D014652', 'Disease') :: ['vascular endothelial dysfunction'] @entity19 :: ('10090', 'Species') :: ['mice', 'murine'] @entity52905 :: ('16800(Tax:10090)', 'Gene') :: ['GEF-H1'] @entity794 :: ('1280', 'Species') :: ['Staphylococcus aureus'] @entity651 :: ('MESH:D055370', 'Disease') :: ['lung injury'] @entity1897 :: ('MESH:D010523', 'Disease') :: ['peripheral MT'] @entity6580 :: ('22329(Tax:10090)', 'Gene') :: ['VCAM1'] @entity8024 :: ('15894(Tax:10090)', 'Gene') :: ['ICAM1'] @entity44753 :: ('MESH:C567137', 'Disease') :: ['MT', 'microtubule'] @entity647 :: ('MESH:D055371', 'Disease') :: ['acute lung injury']
@entity455 :: (MESH:D014652,Disease) :: ['vascular endothelial dysfunction']
