Dysregulation of the cell cycle is important in oncogenesis. We analyzed the potential inactivation of the CIP/KIP family of the cyclin E/CDK/RB pathway by gene promoter hypermethylation in @entity1778 . The methylation-specific polymerase chain reaction (MSP) with primers for methylated (M-MSP) and unmethylated (U-MSP) alleles of the @entity994 , @entity993 , and @entity8755 genes was used to study five leukemic cell lines, 50 @entity3052 ( @entity3052 ) samples, and 25 @entity981 ( @entity981 ) samples. @entity994 was hemizygously methylated in Raji and Jurkat but remained unmethylated in U937, HL60, and NB4. @entity993 was hemizygously methylated in Raji but unmethylated in the other cell lines. @entity8755 was completely methylated in Raji and NB4, hemizygously methylated in U937, and unmethylated in HL60 and Jurkat. At diagnosis, @entity994 methylation was not detected in any case of @entity3052 or @entity981 . @entity993 methylation occurred in 2 (4%) @entity3052 @entity1 and in 1 (4%) @entity981 @entity1 . @entity8755 methylation occurred in 1 (2%) @entity3052 @entity1 and in 1 (4%) @entity981 @entity1 . Therefore, methylation inactivation of the @entity986 /CDK/RB pathway in @entity1778 is infrequent. A review of the literature showed a marked variation in the frequencies of methylation of these genes, which might be attributable to difference in methodologies used to detect gene methylation.

Epigenetic inactivation of the CIP/KIP cell-cycle control pathway in XXXX .

@entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity981 :: ('MESH:D054198', 'Disease') :: ['ALL', 'acute lymphoblastic leukemia'] @entity986 :: ('1029', 'Gene') :: ['INK4'] @entity993 :: ('10671', 'Gene') :: ['p27'] @entity3052 :: ('MESH:D015470', 'Disease') :: ['acute myeloid leukemia', 'AML'] @entity1778 :: ('MESH:D007938', 'Disease') :: ['leukemias', 'leukemia'] @entity8755 :: ('1028', 'Gene') :: ['p57'] @entity994 :: ('1026', 'Gene') :: ['p21']
@entity3052 :: (MESH:D015470,Disease) :: ['acute leukemias']
