Aneuploidy, an abnormal number of chromosomes, has previously been considered irremediable. Here, we report findings that euploid cells increased among cultured aneuploid cells after exposure to the protein @entity68247 , encoded by a @entity1 -specific gene that is ordinarily expressed in preimplantation embryos and occasionally in stem cells. For footprint-free delivery of @entity68247 to cells, we developed @entity68247 synthetic mRNAs and @entity7686 vectors that encode @entity1 @entity68247 . Applying the @entity68247 biologics to established cultures of @entity19 embryonic stem cells, most of which had become aneuploid and @entity5700 , dramatically increased the number of euploid cells within a few days. We then tested the biologics on non-immortalized primary @entity1 fibroblast cells derived from four individuals with Down syndrome   the most frequent @entity1467 . Within weeks after @entity68247 application to the cells in culture, fluorescent in situ hybridization with a chromosome 21-specific probe detected the emergence of up to 24% of cells with only two rather than three copies. High-resolution G-banded chromosomes further showed up to 40% of cells with a normal karyotype. These findings were confirmed by whole-exome sequencing. Similar results were obtained for cells with the trisomy 18 of @entity7440 . Thus a direct, efficient correction of aneuploidy in @entity1 fibroblast cells seems possible in vitro using @entity1 @entity68247 .

Correction of Down syndrome and XXXX in @entity1 cell cultures.

@entity1 :: ('9606', 'Species') :: ['mammalian', 'human'] @entity5700 :: ('MESH:D011123', 'Disease') :: ['polyploid'] @entity7686 :: ('11191', 'Species') :: ['Sendai virus'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity1467 :: ('MESH:D004314', 'Disease') :: ['autosomal trisomy of chromosome 21'] @entity68247 :: ('201516', 'Gene') :: ['ZSCAN4'] @entity7440 :: ('MESH:C580500', 'Disease') :: ['Edwards syndrome']
@entity7440 :: (MESH:C580500,Disease) :: ['Edwards syndrome aneuploidies']
