Prolonged storage of organs for transplant results in tissue damage which may be compounded on reperfusion of the graft tissue. The effect of storage times was examined on hepatic mitochondrial @entity26 consumption and activities of complexes I, II-III, IV, and V in mitochondria isolated from @entity35 liver isografts stored for 25 min and 24 h pre- and posttransplantation. While Complex I activity was significantly (P < 0.05) inhibited under all the conditions studied, @entity3457 was only significantly (P < 0.05) reduced following transplantation of 24-h stored tissue. Complex IV activity remained unchanged under all the conditions studied. Although Complex V activity was significantly damaged within the first 25 min of @entity63 , activity values were partially recovered to control levels following 3 h of reperfusion after transplantation. Prolonged (24 h) storage induced @entity3457 which were irrecoverable. Mitochondria subjected to 25 min @entity63 alone also showed a significant (P < 0.01) decrease in @entity2125 -linked respiratory control indices due to a stimulated state 4 rate. The 24-h storage and transplantation brought about a significantly (P < 0.001) greater inhibition of respiratory control and state 3 respiration. @entity10697 -linked respiration parameters were significantly (P < 0.05) affected in livers subjected to prolonged (24 h) storage or transplantation. These data suggest that a loss of membrane integrity coupled with an inhibition of Complexes I and V and an involvement of Complex II-III in 24-h stored hepatic transplants accounts for mitochondrial @entity1576 in @entity8 . No indication of @entity16 was found in this study. This study shows that damage to specific mitochondrial complexes occurs as a consequence of hypothermic @entity63 .

Impairment of hepatic mitochondrial respiratory function following storage and orthotopic transplantation of XXXX livers.

@entity2125 :: ('MESH:D009243', 'Chemical') :: ['NAD(+)'] @entity8 :: ('MESH:D007674', 'Disease') :: ['hepatic transplantation injury'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemic injury', 'ischemia'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity16 :: ('MESH:D009422', 'Disease') :: ['Complex IV damage'] @entity10697 :: ('MESH:D005182', 'Chemical') :: ['FAD'] @entity1576 :: ('MESH:D012131', 'Disease') :: ['respiratory dysfunction'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity3457 :: ('OMIM:612348', 'Disease') :: ['Complex II-III activity', 'decreases in Complex V activity']
@entity35 :: (10116,Species) :: ['rat']
