BACKGROUND: Evidence implicated the diagnostic significance of microRNAs in whole urine/urine sediments in @entity189 of the bladder (UCB). However, the contaminated blood cells in @entity1 with haematouria significantly altered the expression profiles of urinary microRNA, influencing the test accuracy. METHODS: MicroRNA profiles of the urine supernatants of UCB @entity1 and controls without any @entity5 and profiles of malignant and corresponding normal mucosa tissues from the @entity1 were determined by microRNA microarray and compared to identify differentially expressed microRNAs. The differential expression was verified in the tissues of an independent @entity1 cohort by RT-qPCR. The diagnostic significance of selected microRNAs as biomarkers in the urine supernatant was investigated in the expanded cohorts. RESULTS: @entity31586 and microRNA-125b were down-regulated in the urine supernatants of UCB @entity1 . The degree of down-regulation was associated with the @entity5 grade. A diagnostic model was developed using a combined index of the levels of @entity31586 and microRNA-125b in the urine supernatant with a sensitivity of 86.7%, a specificity of 81.1% and a positive predicted value ( @entity2286 ) of 91.8%. Discriminating between high- and low-grade UCB, the model using the level of microRNA-125b alone exhibited a sensitivity of 81.4%, a specificity of 87.0% and a @entity2286 of 93.4%. CONCLUSIONS: The results revealed a unique microRNA expression signature in the urine supernatants of UCB @entity1 for the development of molecular diagnostic tests. An effective cell-free urinary microRNA-based model was developed using a combined index of the levels of @entity31586 and microRNA-125b to detect UCB with good discriminating power, high sensitivity and high specificity.

Cell-free urinary XXXX and microRNA-125b are diagnostic markers for the non-invasive screening of bladder cancer.

@entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'malignancy'] @entity2286 :: ('12211', 'Species') :: ['PPV'] @entity189 :: ('MESH:D002277', 'Disease') :: ['urothelial carcinoma'] @entity31586 :: ('407055', 'Gene') :: ['MicroRNA-99a', 'microRNA-99a']
@entity31586 :: (407055,Gene) :: ['microRNA-99a']
