@entity3373 is downregulated in various @entity5 including @entity263 . However, the underlying mechanisms of @entity3373 downregulation are not fully understood. Here, we reported that @entity3373 was silenced through DNA hypermethylation and @entity876 mutation status in laser capture microdissected (LCM) @entity263 and matched adjacent normal tissues. In 22 of 27 (81%) prostate tissues, @entity3373 was significantly downregulated in the @entity5 compared with the normal tissues. Further studies on @entity3373 downregulation mechanism showed that @entity3373 is methylated at the promoter region in both @entity263 tissues and 50 different types of @entity5 cell lines. In seven @entity5 cell lines with @entity3373 hypermethylation, @entity11508 treatment dramatically induced @entity3373 expression. Interestingly, we also found a significant correlation between @entity3373 expression and the status of @entity876 gene in both LCM prostate tissues and 47 @entity5 cell lines. In 29 cell lines with mutant @entity876 , @entity3373 levels were downregulated in 28 lines (97%), whereas in 18 cell lines with wild-type @entity876 (WT @entity876 ), @entity3373 levels were downregulated in only 6 lines (33%, P < 0.001). Electrophoretic mobility shift assay showed that @entity876 binds to the @entity876 response element upstream of @entity3373 , but the binding was inhibited by hypermethylation. To further confirm that @entity876 binding to @entity3373 could regulate @entity3373 expression, we transfected WT @entity876 and MUT @entity876 into PC-3 cells and found that @entity3373 is upregulated by WT @entity876 but not with MUTp53. The apoptotic cells are increased after WT @entity876 transfection. In summary, this is the first report documenting that downregulation of @entity3373 is through DNA methylation and @entity876 mutation pathways in @entity263 .

MicroRNA-145 is regulated by DNA methylation and @entity876 gene mutation in XXXX .

@entity263 :: ('MESH:D011471', 'Disease') :: ['prostate cancer'] @entity3373 :: ('406937', 'Gene') :: ['miR-145', 'MiR-145'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancers', 'cancer'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity11508 :: ('MESH:C014347', 'Chemical') :: ["5-aza-2'-deoxycytidine"]
@entity263 :: (MESH:D011471,Disease) :: ['prostate cancer']
