Although growth and body composition traits are quantitative traits of medical and agricultural importance, the genetic and molecular basis of those traits remains elusive. Our previous genome-wide quantitative trait locus (QTL) analyses in an intersubspecific backcross population between C57BL/6JJcl (B6) and wild @entity17415 @entity19 revealed a major growth QTL (named @entity68326 ) on a proximal region of @entity19 chromosome 2. Using the B6.Cg- @entity68326 intersubspecific congenic strain created, we revealed 12 closely linked QTLs for body weight and body composition traits on an approximately 44.1-Mb wild-derived congenic region. In this study, we narrowed down genomic regions harboring three ( @entity68326 .12, @entity68327 and @entity68328 ) of the 12 linked QTLs and searched for possible candidate genes for the QTLs. By phenotypic analyses of F2 intercross populations between B6 and each of four B6.Cg- @entity68326 subcongenic strains with overlapping and non-overlapping introgressed regions, we physically defined @entity68326 .12 affecting body weight to a 3.8-Mb interval (61.5-65.3 Mb) on chromosome 2. We fine-mapped @entity68327 for body length to an 8.0-Mb interval (57.3-65.3) and @entity68328 for abdominal white fat weight to a 2.1-Mb interval (59.4-61.5). The wild-derived allele at @entity68326 .12 and @entity68327 uniquely increased body weight and length despite the fact that the wild @entity19 has a smaller body size than that of B6, whereas it decreased fat weight at @entity68328 . Exome sequencing and candidate gene prioritization suggested that @entity20647 and @entity68329 are putative candidate genes for @entity68326 .12 and that @entity13384 and @entity68330 are putative candidate genes for @entity68328 . These genes had nonsynonymous SNPs, but the SNPs were predicted to be not harmful to protein functions. These results provide information helpful to identify wild-derived quantitative trait genes causing enhanced growth and resistance to @entity28 .

Fine mapping and candidate gene search of quantitative trait loci for growth and XXXX using @entity19 intersubspecific subcongenic intercrosses and exome sequencing.

@entity68328 :: ('100035194(Tax:10090)', 'Gene') :: ['Pbwg1.5'] @entity68329 :: ('50915(Tax:10090)', 'Gene') :: ['Grb14'] @entity28 :: ('MESH:D009765', 'Disease') :: ['obesity'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity20647 :: ('14526(Tax:10090)', 'Gene') :: ['Gcg'] @entity17415 :: ('10091', 'Species') :: ['Mus musculus castaneus'] @entity13384 :: ('17076(Tax:10090)', 'Gene') :: ['Ly75'] @entity68330 :: ('16420(Tax:10090)', 'Gene') :: ['Itgb6'] @entity68326 :: ('112257(Tax:10090)', 'Gene') :: ['Pbwg1'] @entity68327 :: ('493012(Tax:10090)', 'Gene') :: ['Pbwg1.3']
@entity28 :: (MESH:D009765,Disease) :: ['obesity']
