OBJECTIVE: To explore the expression of caveolin-1 ( @entity3895 -1) induced by interleukin-17F/IL-17 receptor C ( @entity4654 / @entity13771 ) in @entity35 pulmonary microvascular endothelial cells (PMVECs). METHODS: Cultured PMVECs were divided into two groups of time-dependent experiment and @entity13771 signal pathway intervention according to the results of time-dependent experimental group: namely peak time of @entity4654 -induced @entity3895 -1 and its phosphorylation (p- @entity3895 -1) expression of PMVEC for formulating the incubation time between @entity4654 and PMVEC in @entity13771 signal pathway intervention group. Time-dependent experiment group: Western blot was used to detect the expression of @entity3895 -1 after @entity4654 stimulation for 0, 0.5, 1.5, 3.0, 6.0, 12.0, 24.0 h. @entity3895 -1 and its phosphorylation expression after @entity4654 challenge for 0, 10, 30, 60, 90, 120 min were evaluated by Western blot. @entity13771 signal pathway intervention group: PMVECs were divided into two groups after a 3-day pre-treatment of siRNA. The first group received a 60-min stimulation of 100 ng/ml @entity4654 before detecting the expression of p- @entity3895 -1 while the second group was subject to a 24-hour stimulation of 100 ng/ml @entity4654 before detection. In addition, control, meaningless-siRNA, @entity13771 -siRNA, @entity4654 and meaningless-siRNA + @entity4654 groups were set as references for two groups respectively. RESULTS: @entity4654 up-regulated the expression of @entity3895 -1 in a time-dependent manner. At 0, 0.5, 1.5, 3.0, 6.0, 12.0, 24.0 h, the relative expression levels of @entity3895 -1 were (1.139    0.134), (1.276    0.166), (1.604    0.080), (2.115    0.231), (2.763    0.226), (3.309    0.493) and (3.963    0.169). At 1.5 h, it was significantly higher than 0 h, peaked at 24.0 h and remained significantly higher than 0, 0.5, 1.5, 3.0, 6.0, 12.0 h (all P < 0.05). And @entity4654 increased the expression of p- @entity3895 -1 in a time-dependent manner. At 0, 10, 30, 60, 90, 120 min, the relative expression levels of p- @entity3895 -1 were (0.540    0.085), (0.880    0.084), (1.437    0.297), (1.491    0.212), (1.017    0.210) and (0.882    0.074). At 10 min, p- @entity3895 -1 was significantly higher than 0 min, peaked at 60 min, remained significantly higher than 0, 10, 30, 90, 120 min (all P < 0.05) and gradually decreased. At 120 min, it was still higher than 0 min. Compared with @entity4654 group, @entity13771 -siRNA significantly inhibited @entity4654 -induced @entity3895 -1 and its phosphorylation (2.126    0.318 vs 3.897    0.424 and 1.014    0.136 vs 1.431    0.298, all P < 0.05). CONCLUSIONS: @entity4654 up-regulates the expressions of @entity3895 -1 and p- @entity3895 -1 in a time-dependent manner in PMVECs. And the silenced expression of @entity13771 in PMVECs by @entity13771 -siRNA significantly inhibits the @entity4654 -induced expressions of @entity3895 -1 and p- @entity3895 -1.

[Effects of IL-17F/IL-17RC on the expression of caveolae-1 in XXXX pulmonary microvascular endothelial cells].

@entity35 :: ('10116', 'Species') :: ['rat'] @entity13771 :: ('84818', 'Gene') :: ['IL-17RC'] @entity4654 :: ('112744', 'Gene') :: ['IL-17F'] @entity3895 :: ('10537', 'Species') :: ['Cav']
@entity35 :: (10116,Species) :: ['rat']
