AIM: To investigate three isoforms of survivin in @entity14 . METHODS: We used the LightCycler Technology (Roche), along with a common forward primer and reverse primers specific for the splice variants and two common hybridization probes labeled with @entity941 and LightCycler-Red fluorophore (LC-Red 640). Real time quantitative polymerase chain reaction (PCR) was performed on cDNAs from 52 @entity5 specimens from @entity14 @entity1 and 10 unrelated normal colorectal tissues. In the @entity1 group, @entity787 ( @entity787 ) and CA19-9 @entity5 markers were also measured immunochemically. RESULTS: Wild type survivin mRNA isoform was expressed in 48% of the 52 @entity5 samples, survivin-2b in 38% and survivin-    x3 in 29%, while no expression was found in normal tissues. The mRNA expression of wild type survivin presented a significant correlation with the expression of the ratio of survivin-2b, survivin-    x3, survivin-2b/wild type survivin and survivin-    x3/wild type survivin (P < 0.001). The mRNA expression of wild-survivin and survivin-    x3 was related with @entity5 size and invasion (P = 0.006 and P < 0.005, respectively). A significant difference was found between survivin-2b and morphologic @entity5 type. Also, the ratio of survivin-  Ex3/wild-survivin was significantly associated with prognosis. No association was observed between the three isoforms and grade, @entity3 , Dukes stage and gender. The three isoforms were not correlated with @entity787 and CA19-9. CONCLUSION: Survivin isoforms may play a role in cell apoptosis and their quantification could provide information about clinical management of @entity1 suffering from @entity14 .

Survivin isoforms and clinicopathological characteristics in XXXX using real-time qPCR.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity787 :: ('1084', 'Gene') :: ['CEA', 'carcinoembryonic antigen'] @entity941 :: ('MESH:D019793', 'Chemical') :: ['fluorescein'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal cancer', 'colorectal adenocarcinomas'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis']
@entity14 :: (MESH:D015179,Disease) :: ['colorectal adenocarcinomas']
