@entity8974 (TB) ( @entity8974 ), a promising oral antifungal agent used worldwide, has been used in the treatment of superficial @entity3217 . In our study, we demonstrated that TB dose-dependently decreased cell number in various cultured @entity1 malignant cells. Flow cytometry analysis revealed that TB interrupts the cell cycle at the G0/G1 transition. The TB-induced cell cycle arrest in @entity694 cell line (COLO 205) occurred when the cyclin-dependent kinase (cdk) system was inhibited just as the levels of @entity876 , @entity994 / @entity994 and @entity5438 / @entity13525 proteins were augmented. In the TB-treated COLO 205, the binding between @entity876 protein and @entity876 consensus binding site in @entity994 / @entity994 promoter DNA probe was increased. Pretreatment of COLO 205 with @entity876 -specific antisense oligodeoxynucleotide decreased the TB-induced elevations of @entity876 and @entity994 / @entity994 proteins, which in turn led to arrest in the cell cycle at the G0/G1 phase. Moreover, in the @entity876 null cells, HL60, TB treatment did not induce cell cycle arrest. Taken together, these results suggest an involvement of the @entity876 -associated signaling pathway in the TB-induced antiproliferation in COLO 205. We further examined whether administration of TB could affect the growth of @entity5 derived from @entity1 @entity694 cells in an in vivo setting. COLO 205 cells implanted subcutaneously in @entity19 formed solid @entity5 ; subsequent intraperitoneal injections of TB (50 mg/kg) led to obvious decline in @entity5 size, up to 50-60%. In these @entity5 , increases in the @entity994 / @entity994 , @entity5438 / @entity13525 and @entity876 proteins and the occurrence of apoptosis were observed. Combined treatment with TB and @entity10612 (ND), a clinically used anticancer agent, potentiated the apoptotic effect in COLO 205. These findings demonstrate for the first time that TB can inhibit the proliferation of @entity5 cells in vitro and in vivo.

In vitro and in vivo studies of the anticancer action of @entity8974 in @entity1 XXXX cell lines: G0/G1 @entity876 -associated cell cycle arrest.

@entity1 :: ('9606', 'Species') :: ['human'] @entity8974 :: ('MESH:C041359', 'Chemical') :: ['Terbinafine', 'Lamisil'] @entity3217 :: ('MESH:D015821', 'Disease') :: ['mycosis'] @entity5438 :: ('3429', 'Gene') :: ['p27'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity19 :: ('10090', 'Species') :: ['nude mice'] @entity10612 :: ('MESH:D015739', 'Chemical') :: ['nocodazole'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity13525 :: ('1027', 'Gene') :: ['Kip1'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon cancer'] @entity994 :: ('1026', 'Gene') :: ['Cip1', 'p21']
@entity5 :: (MESH:D009369,Disease) :: ['cancer']
