UNASSIGNED: Genetic background plays an important role in the development of @entity2074 . A genome-wide association study (GWAS) showed that nine loci are associated with the disease, six of which contain genes that are involved in Wnt signaling ( @entity17731 , @entity18182 , @entity13023 , @entity43125 , @entity18181 , @entity19922 ). To obtain insight in the role of these genes, we performed expression studies on affected and unaffected @entity1 's tissues. Surgically obtained nodules and cords from eight Dupuytren's @entity1 were compared to @entity1 -matched control tissue (unaffected transverse palmar fascia). The Wnt-related genes found in the GWAS, the classical Wnt-downstream protein @entity3546 , as well as (myo)fibroblast markers were analyzed using real-time qPCR and immunohistochemical stainings for mRNA levels and protein levels, respectively. The collagen-coding genes @entity13239 and @entity1414 were highly upregulated on mRNA level, both in cords and nodules. Three Wnt-related genes were found to be differently regulated compared to control tissue: @entity17731 was downregulated in nodules, @entity13023 was upregulated in nodules, and @entity18181 was upregulated in nodules and cords. Immunohistochemistry revealed significantly less staining of @entity17731 in cords, but significantly more staining for @entity13023 in nodules. There was significantly more staining of @entity1970 in nodules and cord and @entity3546 in nodules than in control tissue. We found differences in expression, both at mRNA and protein level, in several Wnt-related genes found earlier to be associated with @entity2074 . Of these, @entity13023 was upregulated and found in close association with both @entity1970 and @entity3546 expressing cells, making it a candidate pro-fibrotic mediator in @entity2074 .

Further evidence of the involvement of the Wnt signaling pathway in XXXX .

@entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity1414 :: ('1281', 'Gene') :: ['COL3A1'] @entity17731 :: ('7472', 'Gene') :: ['Wnt2', 'WNT2'] @entity19922 :: ('23213', 'Gene') :: ['SULF1'] @entity18182 :: ('54361', 'Gene') :: ['WNT4'] @entity18181 :: ('6424', 'Gene') :: ['SFRP4'] @entity13239 :: ('1277', 'Gene') :: ['COL1A1'] @entity1970 :: ('58', 'Gene') :: ['a-SMA'] @entity13023 :: ('7477', 'Gene') :: ['Wnt7b', 'WNT7B'] @entity3546 :: ('1499', 'Gene') :: ['b-catenin'] @entity2074 :: ('MESH:D004387', 'Disease') :: ["Dupuytren's disease"] @entity43125 :: ('340419', 'Gene') :: ['RSPO2']
@entity2074 :: (MESH:D004387,Disease) :: ['Dupuytren's disease']
