In this study, four different laboratory scale gluten-free (GF) sourdoughs were developed from buckwheat or teff flours. The fermentations were initiated by the spontaneous biota of the flours and developed under two technological conditions (A and B). Sourdoughs were propagated by continuous back-slopping until the stability was reached. The composition of the stable biota occurring in each sourdough was assessed using both culture-dependent and -independent techniques. Overall, a broad spectrum of @entity880 bacteria (LAB) and @entity375 species, belonging mainly to the genera @entity1336 , @entity26044 , Leuconostoc, Kazachstania and Candida, were identified in the stable sourdoughs. Buckwheat and teff sourdoughs were dominated mainly by obligate or facultative heterofermentative LAB, which are commonly associated with traditional @entity3505 or rye sourdoughs. However, the spontaneous fermentation of the GF flours resulted also in the selection of species which are not consider endemic to traditional sourdoughs, i.e. @entity26044 , Leuconostoc holzapfelii, @entity1336 gallinarum, @entity1336 vaginalis, @entity5210 , @entity1336 graminis and @entity26043 . In general, the composition of the stable biota was strongly affected by the fermentation conditions, whilst @entity1336 dominated in all buckwheat sourdoughs. @entity1336 pontis is described for the first time as dominant species in teff sourdough. Among @entity375 , @entity375 and @entity7408 dominated teff sourdoughs, whereas the solely Kazachstania barnetti was isolated in buckwheat sourdough developed under condition A. This study allowed the identification and isolation of LAB and @entity375 species which are highly competitive during fermentation of buckwheat or teff flours. Representatives of these species can be selected as starters for the production of sourdough destined to GF bread production.

Biodiversity of XXXX bacteria and @entity375 in spontaneously-fermented buckwheat and teff sourdoughs.

@entity3505 :: ('4565', 'Species') :: ['wheat'] @entity7408 :: ('5478', 'Species') :: ['Candida glabrata'] @entity26044 :: ('1255', 'Species') :: ['Pediococcus', 'Pediococcus pentosaceus'] @entity5210 :: ('1599', 'Species') :: ['Lactobacillus sakei'] @entity26043 :: ('137591', 'Species') :: ['Weissella cibaria'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactic acid'] @entity1336 :: ('1590', 'Species') :: ['Lactobacillus', 'Lactobacillus plantarum'] @entity375 :: ('4932', 'Species') :: ['yeasts', 'Saccharomyces cerevisiae']
@entity880 :: (MESH:D019344,Chemical) :: ['lactic acid']
