Recent reports indicate that the cytochrome P450 isozyme, @entity37453 , is expressed at high levels in the inner regions of the @entity840 adrenal cortex and may contribute to xenobiotic and/or @entity134 metabolism in the gland. In the present studies, immunohistochemical and in situ hybridization techniques were employed to definitively establish the localization of @entity37453 within the adrenal cortex. In male @entity840 of various ages, @entity37453 protein and mRNA were highly localized to the @entity4091 ( @entity4091 ); none was detectable in the @entity4091 ( @entity4091 ), @entity4091 ( @entity4091 ) or the medulla. In contrast, the steroidogenic P450 isozyme, @entity37452 , was distributed throughout the @entity4091 and @entity4091 . From the earliest stages of development of the @entity4091 , @entity37453 staining was intense. As @entity840 aged, the @entity4091 progressively enlarged and comprised a proportionately greater amount of the cortex. At all ages, @entity37453 was uniformly distributed throughout only the @entity4091 . Coinciding with the age-related growth of the @entity4091 and increase in adrenal @entity37453 content was an increase in adrenal xenobiotic-metabolizing activity. The results establish that @entity37453 has an intraadrenal localization that is unique among P450 isozymes, suggesting novel regulatory mechanisms and indicating that @entity37453 may serve as a specific marker for @entity4091 cells. The increase in @entity37453 expression with age probably accounts for increasing levels of xenobiotic metabolism and may also contribute to an increase in intraadrenal @entity701 degradation in older animals.

Localization of @entity37453 in the XXXX adrenal cortex by immunohistochemistry and in situ hybridization.

@entity37453 :: ('100169696(Tax:10141)', 'Gene') :: ['CYP2D16'] @entity840 :: ('10141', 'Species') :: ['guinea pigs', 'guinea pig'] @entity37452 :: ('100729670', 'Gene') :: ['CYP17'] @entity4091 :: ('MESH:D006562', 'Disease') :: ['zona glomerulosa', 'ZR', 'zona reticularis', 'zona fasciculata', 'ZG', 'ZF'] @entity134 :: ('CHEBI:35341', 'Chemical') :: ['steroid'] @entity701 :: ('MESH:D006854', 'Chemical') :: ['cortisol']
@entity840 :: (10141,Species) :: ['guinea pig']
