Pitanga (Eugenia uniflora L.) is a member of the Myrtaceae family and is of particular interest due to its medicinal properties that are attributed to specialized metabolites with known biological activities. Among these molecules, @entity6399 are the most abundant in essential oils that are found in the leaves and represent compounds with potential pharmacological benefits. The @entity9534 diversity observed in Myrtaceae is determined by the activity of different members of the @entity9534 synthase and @entity56107 cyclase families. Therefore, the aim of this study was to perform a de novo assembly of transcripts from E. uniflora leaves and to annotation to identify the genes potentially involved in the @entity25164 biosynthesis pathway and @entity9534 diversity. In total, 72,742 unigenes with a mean length of 1048bp were identified. Of these, 43,631 and 36,289 were annotated with the NCBI non-redundant protein and Swiss- @entity8109 databases, respectively. The gene ontology categorized the sequences into 53 functional groups. A metabolic pathway analysis with KEGG revealed 8,625 unigenes assigned to 141 metabolic pathways and 40 unigenes predicted to be associated with the biosynthesis of @entity6399 . Furthermore, we identified four putative full-length @entity9534 synthase genes involved in @entity200 and @entity199 biosynthesis, and three putative full-length @entity56107 cyclase genes involved in the @entity10114 biosynthesis. The expression of these genes was validated in different E. uniflora tissues.

De novo assembly of Eugenia uniflora L. transcriptome and identification of genes from the XXXX biosynthesis pathway.

@entity6399 :: ('MESH:D013729', 'Chemical') :: ['terpenoids'] @entity8109 :: ('6534', 'Gene') :: ['Prot'] @entity199 :: ('MESH:D039821', 'Chemical') :: ['monoterpenes'] @entity9534 :: ('CHEBI:35186', 'Chemical') :: ['terpene'] @entity10114 :: ('MESH:D014315', 'Chemical') :: ['triterpenes'] @entity25164 :: ('CHEBI:26873', 'Chemical') :: ['terpenoid'] @entity200 :: ('MESH:D012717', 'Chemical') :: ['sesquiterpenes'] @entity56107 :: ('MESH:C520393', 'Chemical') :: ['oxidosqualene']
@entity25164 :: (CHEBI:26873,Chemical) :: ['terpenoid']
