BACKGROUND/AIM: Lymphatic and blood vessel invasion are important independent prognostic factors in @entity14 , but identification of the separate components remains difficult. The aim of the present study was to compare routine @entity1710 and @entity1711 (H_E) and elastica staining with immunohistochemistry using D2-40 and @entity2279 . MATERIALS AND METHODS: A total of 75 surgical specimens of @entity14 were examined for blood and lymphatic vessel invasion, by comparing stains. RESULTS: The minimum clinical follow-up of survivors was 5 years. During that time, 45 @entity1 died, 34 from their @entity5 . Lymphatic invasion by H_E was found in 19% compared to 40% detected with D2-40 (p<0.001). Lymphatic invasion was not associated with @entity5 (H_E, p=0.923; D2-40, p=0.724) but was significantly associated with N-stage, (H_E, p=0.001; D2-40, p<0.001). No significant association between lymphatic invasion (H_E or D2-40) and @entity5 -specific survival was found on univariate analysis. Blood vessel invasion by elastic detection was detected in 53% compared to 32% detected with @entity2279 (p=0.090). Blood vessel invasion was associated with @entity5 , (elastica, p=0.028; @entity2279 , p=0.839) but was not associated with N-stage (elastica, p=0.377; @entity2279 , p=0.519). On univariate analysis of blood vessel invasion was associated with @entity5 -specific survival (elastica, p=0.009) when detected by elastica, but not when detected by @entity2279 , (p=0.611). Lymphatic invasion (D2-40) was associated with blood vessel invasion (elastic) (p=0.019). On multivariate analysis, blood vessel invasion with elastica had independent prognostic value (hazard ratio=2.55, 95% confidence interval=1.23-5.28; p=0.012). CONCLUSION: The results of the present study indicate that immunohistochemistry using D2-40 improves the identification of lymphatic invasion compared to use of H_E staining only; however, its prognostic value was limited. Elastica staining improves the detection rate of blood vessel invasion (compared to @entity2279 ) and venous invasion detected with elastica had independent prognostic value in @entity1 undergoing curative resection for @entity14 .

Comparison of Methods to Identify Lymphatic and Blood Vessel Invasion and their Prognostic Value in @entity1 with Primary Operable XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['T-stage', 'cancer'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity2279 :: ('5175', 'Gene') :: ['CD31'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hematoxylin'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal cancer']
@entity14 :: (MESH:D015179,Disease) :: ['Colorectal Cancer']
