In higher plants, the terminal step of @entity620 ( @entity1281 ) biosynthesis is catalyzed by the enzyme @entity69920 dehydrogenase (EC 1.3.2.3, GalLDH). We generated @entity1281 -deficient transgenic @entity286 BY-2 cell lines by antisense expression of the GalLDH cDNA that was amplified from BY-2 cells using PCR. Two transgenic cell-lines, AS1-1 and AS2-2, having a marked expression of antisense RNA were analyzed. Antisense suppression of GalLDH mRNA led to a significant decline in the GalLDH activity. The @entity1281 levels in the transgenic cell lines were found to be 30% lower than the wild-type BY-2 cells. In synchronous cultures, division of @entity19 and @entity19 cells was restrained with a concomitant decrease in mitotic index that was probably due to a decline in @entity1281 levels. The rate of cell growth was also found to be less than that of the wild-type cells. Interestingly, there was a significant phenotypic difference between the transgenic and wild-type cells. The calli of AS1-1 and AS2-2 appeared to be sticky and soft. Back extrusion method also showed that @entity1281 -deficient BY-2 callus was rheologically soft. Furthermore, microscopic analysis revealed that @entity19 and @entity19 cells were abnormally slender, suggesting a potential for a significant and a uni-axial elongation. Thus, we observed that decline in the @entity1281 levels has an adverse effect on the division, growth and structure of a plant cell.

Generation and properties of @entity620 -deficient transgenic XXXX cells expressing antisense RNA for @entity69920 dehydrogenase.

@entity286 :: ('4097', 'Species') :: ['tobacco'] @entity620 :: ('MESH:D001205', 'Chemical') :: ['L-ascorbic acid'] @entity19 :: ('10090', 'Species') :: ['AS1-1', 'AS2-2'] @entity69920 :: ('MESH:C534014', 'Chemical') :: ['L-galactono-1,4-lactone'] @entity1281 :: ('CHEBI:15365', 'Chemical') :: ['AsA']
@entity286 :: (4097,Species) :: ['tobacco']
