UNASSIGNED: @entity2648 are added as a preservative to a variety of cured meats, including bacon, to kill bacteria, extend shelf-life, and improve quality. During cooking, @entity2648 in the meat can be converted to carcinogenic @entity15028 ( @entity144 ), the formation of which are mitigated by the addition of anti-oxidants. In the past, the United States Department of Agriculture (USDA) Food Safety and Inspection Service (FSIS) monitored @entity144 in pan-fried bacon, but FSIS terminated monitoring of @entity144 in the 1990s due to the very low levels found. FSIS recently chose to conduct a risk assessment of @entity144 in cooked bacon to determine if current levels warrant routine monitoring of @entity144 again. To meet FSIS needs, we developed, validated, and implemented a new method of sample preparation and analysis to test cooked bacon for 5 @entity144 of most concern, which consist of @entity1232 , - @entity21677 , - @entity67406 , - @entity23966 , and - @entity29276 . Sample preparation was based on the QuEChERS (quick, easy, cheap, effective, rugged, and safe) approach and analysis by gas chromatography - tandem mass spectrometry. Ruggedness was improved markedly in the analysis of the complex fatty extracts by backflushing the guard column, injection liner, and half of the analytical column after every injection. Validation results were acceptable with recoveries of 70-120% and <20% RSDs for the 5 @entity144 , with a reporting limit of 0.1 ng/g. NA concentrations in 48 samples were all <15 ng/g, with @entity30333 ng/g and many <0.1 ng/g. Also, microwave cooking of bacon gave slightly lower concentrations overall compared to pan-frying.

Analysis of XXXX in cooked bacon by QuEChERS sample preparation and gas chromatography - tandem mass spectrometry with backflushing.

@entity15028 :: ('MESH:D009602', 'Chemical') :: ['nitrosamines'] @entity144 :: ('MESH:C015378', 'Chemical') :: ['NAs'] @entity30333 :: ('100507249', 'Gene') :: ['most <1'] @entity21677 :: ('MESH:C034281', 'Chemical') :: ['diethylamine'] @entity29276 :: ('MESH:C032519', 'Chemical') :: ['pyrrolidine'] @entity1232 :: ('MESH:D004128', 'Chemical') :: ['N-nitroso -dimethylamine'] @entity23966 :: ('MESH:C032727', 'Chemical') :: ['piperidine'] @entity67406 :: ('MESH:C045274', 'Chemical') :: ['dibutylamine'] @entity2648 :: ('MESH:D009573', 'Chemical') :: ['Nitrites', 'nitrites']
@entity15028 :: (MESH:D009602,Chemical) :: ['nitrosamines']
