INTRODUCTION: Endothelium dysfunction plays a critical role in @entity731 . MicroRNAs are endogenous non-coding RNAs that suppress gene expression by binding to the 3' untranslated regions of target genes. @entity21072 can regulate the proliferation and apoptosis of @entity5 cells, however, the roles of @entity21072 in endothelial cells (ECs) remain unclear. Therefore, this study aims to investigate the roles and mechanisms of @entity21072 on ECs proliferation and apoptosis. MATERIALS AND METHODS: @entity21072 and @entity2842 expressions were examined using quantitative RT-PCR, ELISA assay and western blot. Bioinformatics analysis and luciferase reporter assay were used to examine the regulatory relationship between @entity21072 and @entity2842 . CCK8 assay, @entity1603 incorporation assay and flow cytometry were used to analyze the roles of @entity21072 and @entity2842 on the proliferation of @entity1 umbilical vein endothelial cells (HUVECs). The effects of @entity21072 and @entity2842 on HUVECs apoptosis were examined by tunnel staining and western blot. RESULTS: @entity21072 was down-regulated in @entity1 with @entity453 compared with healthy controls. @entity2842 was a novel target gene of @entity21072 . @entity21072 significantly promoted HUVECs proliferation by altering cell cycle distribution, and it also inhibited HUVECs apoptosis by affecting the expression of cleaved caspase 3. Effects of @entity21072 on HUVECs proliferation and apoptosis were significantly reversed by overexpression of @entity2842 . CONCLUSIONS: @entity21072 could affect HUVECs proliferation and apoptosis by directly targeting @entity2842 . This is the first report to disclose the roles and mechanisms of @entity21072 on HUVECs proliferation and apoptosis, which may provide a theoretical basis for clarifying the mechanisms of @entity731 .

MicroRNA-495 regulates the proliferation and apoptosis of @entity1 umbilical vein endothelial cells by targeting chemokine XXXX .

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity1603 :: ('MESH:D001973', 'Chemical') :: ['BrdU'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity2842 :: ('6347', 'Gene') :: ['CCL2'] @entity453 :: ('MESH:D003324', 'Disease') :: ['coronary artery disease'] @entity21072 :: ('574453', 'Gene') :: ['MiR-495', 'miR-495'] @entity731 :: ('MESH:D050197', 'Disease') :: ['atherosclerosis']
@entity2842 :: (6347,Gene) :: ['CCL2']
