UNASSIGNED: 31 Background: New biomarkers are needed to better predict outcome after radical prostatectomy for clinically @entity263 . Recent data suggest that subpopulations of @entity5 stem-like cells (CSC) are present in @entity263 , and the CSC phenotype has been associated with high tumorigenicity, drug resistance, and disease progression. We sought to determine whether the expression of genes associated with the CSC phenotype are associated with disease recurrence. METHODS: A nested case-control study was performed. Cases were @entity1 who underwent radical prostatectomy and had disease recurrence (n=147). Controls were @entity1 who underwent radical prostatectomy and did not have disease recurrence (n=59). Cases and controls were matched based on D'Amico risk stratification group. Laser capture microdissection with mRNA extraction and quantitative RT-PCR were used to measure the expression of 9 novel candidate CSC-associated genes ( @entity18609 , @entity3752 , @entity5439 , CD44a, @entity3546 , @entity12974 , @entity13455 , @entity125 , @entity3309 ) in @entity406 -fixed, paraffin-embedded radical prostatectomy specimens. The Wilcoxon test was used to examine the association between CSC-associated gene expression and D'Amico risk stratification group. The optimal chi-square approach and recursive partitioning analysis were used to determine the association between CSC-associated gene expression and disease recurrence. RESULTS: Complete gene expression data were evaluable for 206 out of 261 @entity1 (79%). The median age was 64 years (range, 42-78 years). High expression of @entity3309 (p=0.04) and CD44a (p=0.05) were associated with D'Amico low risk group. The optimal chi-square approach showed that @entity18609 was associated with disease recurrence (recurrence rate: @entity18609 <=  3.83 = 80% versus @entity18609 > 3.83 = 43%, p=0.0015). Recursive partitioning analysis confirmed an association between @entity18609 and disease recurrence. CONCLUSIONS: A novel finding of the current study was that a low gene expression level of @entity18609 was associated with disease recurrence. High gene expression levels of @entity3309 and CD44a were associated with D'Amico low risk group. Further analysis of the Wnt/ @entity3546 pathway is ongoing in this cohort.

Association between @entity5 stem-like cell gene expression and clinical outcome in XXXX : A nested case-control study.

@entity1 :: ('9606', 'Species') :: ['patients', 'men'] @entity263 :: ('MESH:D011471', 'Disease') :: ['localized prostate cancer', 'prostate cancer'] @entity5439 :: ('8842', 'Gene') :: ['CD133'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity3752 :: ('648', 'Gene') :: ['Bmi1'] @entity13455 :: ('4824', 'Gene') :: ['Nkx3-1'] @entity18609 :: ('8313', 'Gene') :: ['Axin2'] @entity406 :: ('MESH:D005557', 'Chemical') :: ['formalin'] @entity3546 :: ('1499', 'Gene') :: ['beta-catenin', 'CTNNB1'] @entity125 :: ('4851', 'Gene') :: ['Notch1'] @entity3309 :: ('4070', 'Gene') :: ['TACSTD2'] @entity12974 :: ('3673', 'Gene') :: ['ITGA2']
@entity263 :: (MESH:D011471,Disease) :: ['localized prostate cancer']
