BACKGROUND: Elevated fasting levels of branched chain @entity3534 (BCAAs: @entity4857 , @entity6778 , @entity368 ) in venous blood are associated with a variety of @entity209 , including increased risk of @entity2175 ( @entity2175 ). Fasting @entity18200 levels are influenced by non-dietary factors. However, it is unknown whether fasting BCAAs can be altered through manipulation of dietary intake alone. OBJECTIVE: To test whether a specific dietary intervention, using differences in @entity18200 intake, alters fasting @entity18200 levels independent of other factors. DESIGN: Five healthy male volunteers underwent 4 days of a low and 4 days of a high @entity18200 content dietary intervention (ClinicalTrials.gov [NCT02110602]). All food and supplements were provided. Fasting BCAAs were measured from venous blood samples by mass spectrometry at baseline and after each intervention. RESULTS: Diets were isocaloric; contained equal percentages of calories from @entity1735 , fats, and protein; and differed from each other in @entity18200 content (1.5  0.1 vs. 14.0  0.6 g for @entity4857 ; 4.5  0.9 g vs. 13.8  0.5 g for @entity6778 ; 2.1  0.2 g vs. 27.1  1.0 g for @entity368 ; p<0.0001 for all). Fasting @entity4857 was significantly lower (p=0.02) and fasting @entity6778 and @entity368 were numerically lower following the low @entity18200 content vs. the high @entity18200 content diet levels. The inter-individual response to the dietary interventions was variable and not explained by adherence. CONCLUSION: Short-term dietary manipulation of @entity18200 intake led to modest changes in fasting levels of BCAAs. The approach from our pilot study can be expanded to test the metabolic implications of dietary @entity18200 manipulation.

A pilot, short-term dietary manipulation of branched chain @entity3534 has modest influence on fasting levels of branched chain XXXX .

@entity3534 :: ('MESH:D000596', 'Chemical') :: ['amino acids'] @entity2175 :: ('MESH:D003924', 'Disease') :: ['type 2 diabetes', 'T2D'] @entity18200 :: ('51742', 'Gene') :: ['BCAA'] @entity368 :: ('CHEBI:25017', 'Chemical') :: ['leucine'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['carbohydrate'] @entity6778 :: ('CHEBI:24898', 'Chemical') :: ['isoleucine'] @entity4857 :: ('CHEBI:27266', 'Chemical') :: ['valine'] @entity209 :: ('MESH:D008659', 'Disease') :: ['metabolic impairments']
@entity3534 :: (MESH:D000596,Chemical) :: ['amino acids']
