UNASSIGNED: The miR-19 family ( @entity34947 and @entity38205 ) are key oncogenic components of the @entity10014 cluster. Overexpression of miR-19 is strongly associated with @entity5 invasion and @entity3 , and poor prognosis of @entity5 @entity1 . However, the underlying mechanisms remain largely unknown. In the present study, we found that enforced expression of miR-19 including @entity34947 and @entity38205 triggered epithelial-mesenchymal transition (EMT) of @entity2 cells A549 and HCC827 as shown by mesenchymal-like morphological conversion, downregulation of epithelial proteins (e.g., @entity5962 , @entity11178 ), and a-catenin), upregulation of mesenchymal proteins (e.g., @entity2277 , @entity8946 , @entity6076 , and @entity4245 ), formation of stress fibers, and reduced cell adhesion. In addition, enhanced @entity4222 and invasion were observed in the @entity5 cells A549 and HCC827 undergoing EMT. In contrast, silencing of endogenous miR-19 reversed EMT and reduced the @entity4222 and invasion abilities of A549 and @entity1 cells. DNA microarray results revealed significant changes of the expression of genes related to EMT, @entity4222 , and @entity3 of miR-19-expressing A549 cells. Moreover, siRNA-mediated knockdown of @entity983 , a target of miR-19, also resulted in EMT, @entity4222 , and invasion of A549 and @entity1 cells, suggesting that @entity983 is involved in miR-19-induced EMT, @entity4222 and invasion of @entity2 cells. Furthermore, @entity2 cells undergoing EMT induced by miR-19 demonstrated reduced proliferation in vitro and in vivo, and enhanced resistance to apoptosis caused by @entity310 . Taken together, these findings suggest that miR-19 triggers EMT, which has an important role in the invasion and @entity2 cells, accompanied by the reduced proliferation of cells.Laboratory Investigation advance online publication, 22 June 2015; doi:10.1038/labinvest.2015.76.

MicroRNA-19 triggers epithelial-mesenchymal transition of XXXX cells accompanied by growth inhibition.

@entity1 :: ('9606', 'Species') :: ['HCC827', 'patients'] @entity11178 :: ('7082', 'Gene') :: ['ZO-1 (zona occludens 1'] @entity34947 :: ('406979', 'Gene') :: ['miR-19a'] @entity10014 :: ('407975', 'Gene') :: ['miR-17-92'] @entity4245 :: ('6615', 'Gene') :: ['snail1'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity4222 :: ('MESH:D014085', 'Disease') :: ['migration'] @entity310 :: ('7124', 'Gene') :: ['TNF-a'] @entity2 :: ('MESH:D008175', 'Disease') :: ['migration of lung cancer', 'lung cancer'] @entity5962 :: ('999', 'Gene') :: ['E-cadherin'] @entity983 :: ('5728', 'Gene') :: ['PTEN'] @entity2277 :: ('7431', 'Gene') :: ['vimentin'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity8946 :: ('2335', 'Gene') :: ['fibronectin 1'] @entity6076 :: ('1000', 'Gene') :: ['N-cadherin'] @entity38205 :: ('406980', 'Gene') :: ['miR-19b-1']
@entity2 :: (MESH:D008175,Disease) :: ['lung cancer']
