@entity1951 and @entity398 neuroendocrine cells within the @entity2339 ( @entity4525 ) display mRNA expression for the @entity143 receptor subunits, @entity4474 and @entity4319 , @entity4477 and @entity4475 . The @entity4319 subunit confers slow decay kinetics (relative to @entity4474 / @entity4476 receptors) and high @entity1760 sensitivity to @entity143 receptor responses--properties which may contribute to the @entity143 receptor-mediated bursting manifested by these cells. Therefore, we examined @entity4319 protein expression and its developmental profile in the @entity4525 and compared it to that in the cortex and cerebellum--areas which have been studied previously. We performed Western blot analysis on @entity4525 homogenates from embryonic, postnatal (PN7, 14, 21), and adult @entity35 using an @entity4319 -specific antibody. Adult @entity4319 levels in the @entity4525 and PVN were similar but low relative to those of cortex. @entity4525 @entity4319 protein levels rose in the first postnatal week, remained high through PN21, and later declined to significantly lower levels in the adult. A similar profile was observed in cerebellum, where @entity4319 expression displayed a sharp peak at PN14 and later declined to minimal or undetectable levels in the adult. In contrast, @entity4319 continued to be overexpressed through adulthood in the cortex. The ontogenic pattern for @entity4474 expression, which included unregulation during early postnatal life and adulthood, was similar in the @entity4525 and cortex. A different pattern was observed for the cerebellum, where @entity4474 levels increased gradually after ED17 to reach significantly greater adult levels. Of all three areas studied, the @entity4525 displayed the earliest developmental rise in @entity4474 levels. @entity4525 explant cultures proved to be a useful preparation, since they contained neurons which synthesized @entity4474 and @entity4319 subunits in quantities similar to those of ED17 @entity4525 . Our findings suggest that @entity143 receptors on @entity4525 neuroendocrine cells are assembled using @entity4474 and @entity4319 subunits, and that their plastic expression in early postnatal life may play a role during development.

Developmental plasticity of @entity4474 and XXXX subunit expression in the supraoptic nucleus of the @entity35 hypothalamus.

@entity398 :: ('MESH:D010121', 'Chemical') :: ['oxytocin'] @entity4477 :: ('24411(Tax:10116)', 'Gene') :: ['NR2C'] @entity4319 :: ('24410(Tax:10116)', 'Gene') :: ['NR2B'] @entity4475 :: ('24412(Tax:10116)', 'Gene') :: ['NR2D'] @entity4476 :: ('24409(Tax:10116)', 'Gene') :: ['NR2A'] @entity1951 :: ('MESH:D014667', 'Chemical') :: ['Vasopressin'] @entity4474 :: ('24408(Tax:10116)', 'Gene') :: ['NR1'] @entity2339 :: ('MESH:D007029', 'Disease') :: ['supraoptic nucleus of the adult hypothalamus'] @entity143 :: ('CHEBI:6121', 'Chemical') :: ['NMDA'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity4525 :: ('304092(Tax:10116)', 'Gene') :: ['SON'] @entity1760 :: ('MESH:D008274', 'Chemical') :: ['magnesium']
@entity4319 :: (24410(Tax:10116),Gene) :: ['NR2B']
