PURPOSE: To describe the morphological distribution of host tissue and microbial biofilms on the intraocular surfaces of Boston type I keratoprostheses (KPros) explanted because of corneal melt. METHODS: Retrospective study of scanning electron microscopy (SEM) images from 4 explanted Boston type I KPros composed of @entity4625 and @entity702 . SEM images of KPro-associated ocular surfaces were reviewed for the presence of inflammatory cells, microbes, and/or biofilm formation. One sterile type I KPro was also imaged to serve as a (device only) control. RESULTS: All 4 KPros were explanted because of culture-negative, clinically "sterile" donor corneal melt with impending KPro extrusion. In all cases, the rough, irregular surfaces of the device harbored more adherent corneal epithelium and stromacytes, inflammatory cells, and bacteria than the smooth, polished surface of the KPro optic. Two KPros showed not only evidence of prior bacterial colonization but marked biofilm formation. CONCLUSIONS: SEM images of explanted KPros explanted for "sterile" corneal melt demonstrated evidence of biofilm formation despite negative donor corneal cultures and the absence of clinical suspicion for @entity281 . These results suggest that "sterile" corneal melt may be due to inflammatory host responses to low microbial burdens as seen in biofilms and/or released antigens after antibiotic-induced lysis. There was increased adherence of host tissue cells and microbial biofilms on the nonpolished surfaces of the KPro. Polishing the intraocular polymethylmethacrylate and @entity702 KPro surfaces may decrease microbial adhesion and biofilm formation in @entity1 subjects with KPros, but what impact this will have on rates of @entity86 is unknown.

Scanning Electron Microscopic Analysis of Biofilm Formation in Explanted XXXX Boston Type I Keratoprostheses.

@entity4625 :: ('MESH:D019904', 'Chemical') :: ['polymethylmethacrylate'] @entity1 :: ('9606', 'Species') :: ['human'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity86 :: ('MESH:D019106', 'Disease') :: ['postoperative endophthalmitis'] @entity702 :: ('MESH:D014025', 'Chemical') :: ['titanium']
@entity1 :: (9606,Species) :: ['Human']
