BACKGROUND/AIMS: Bone marrow-derived mesenchymal stem cells (BMSCs) have been confirmed to have capacity to differentiate toward hepatic myofibroblasts, which contribute to fibrogenesis in chronic @entity409 . @entity12097 (PPARx03B3;), a ligand-activated transcription factor, has gained a great deal of recent attention as it is involved in @entity82 and cell differentiation. However, whether it regulates the differentiation of BMSCs toward myofibroblasts remains to be defined. METHODS: @entity4314 or bile duct ligation was used to induce @entity19 @entity1575 . Expressions of PPARx03B3;, a-smooth muscle actin, collagen a1 (I) and collagen a1 (III) were detected by real-time RT-PCR and Western blot or immunofluorescence assay. RESULTS: PPARx03B3; expression was decreased in @entity19 fibrotic liver. In addition, PPARx03B3; was declined during the differentiation of BMSCs toward myofibroblasts induced by transforming growth factor b1. Activation of PPARx03B3; stimulated by natural or synthetic ligands suppressed the differentiation of BMSCs. Additionally, knock down of PPARx03B3; by siRNA contributed to BMSC differentiation toward myofibroblasts. Furthermore, PPARx03B3; activation by natural ligand significantly inhibited the differentiation of BMSCs toward myofibroblasts in liver fibrogenesis and alleviated @entity1575 . CONCLUSIONS: PPARx03B3; negatively regulates the differentiation of BMSCs toward myofibroblasts, which highlights a further mechanism implicated in the BMSC differentiation.

XXXX Negatively Regulates the Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells Toward Myofibroblasts in Liver Fibrogenesis.

@entity12097 :: ('19016(Tax:10090)', 'Gene') :: ['Peroxisome proliferator-activated receptor gamma'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis'] @entity4314 :: ('MESH:D002251', 'Chemical') :: ['Carbon tetrachloride'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity409 :: ('MESH:D008107', 'Disease') :: ['liver diseases'] @entity1575 :: ('MESH:D008103', 'Disease') :: ['liver fibrosis']
@entity12097 :: (19016(Tax:10090),Gene) :: ['Peroxisome Proliferator-Activated Receptor Gamma']
