The Tenuivirus @entity2829 stripe virus (MStV) shares many properties with viruses in the genus Phlebovirus of the family Bunyaviridae. Besides genome organization and gene expression strategies, one property shared by these plant- and vertebrate-infecting viruses is that transcription gives rise to virus-specific mRNAs containing nonviral 5'-terminal @entity377 sequences. The 5'-terminal @entity2036 are believed to be derived from host mRNA sequences as a result of "cap-snatching." We investigated whether specific @entity377 sequences could serve as primer donors for cap-snatching in vivo. @entity2247 ( @entity2247 ) plants were singly and doubly infected with MStV and the Hordeivirus @entity18084 ). A reverse transcription-PCR assay was used to identify chimeric @entity18084 /MStV RNAs. Specific reverse transcription-PCR products were detected from doubly infected plants by using one PCR primer corresponding to the 5' termini of the @entity18084 RNAs (alpha, beta, and gamma) and a second primer complementary to MStV RNA 4. The resulting cDNAs were cloned, and @entity377 sequence analysis showed them to be chimeric, containing @entity18084 5'-terminal sequences as well as MStV RNA 4 sequences. All clones contained the @entity18084 RNA 5' primer @entity377 sequence, but they also showed characteristics common to Tenuivirus mRNAs. More than 80% of the clones contained @entity18084 RNA nucleotides not present on the PCR primer. Several lacked the exact 5' terminus of MStV RNA 4, a feature also seen for viruses in the Bunyaviridae. These data show that heterologous virus RNAs ( @entity18084 ) can serve as primer donors for MStV mRNA capped RNA-primed transcription in doubly infected plants.

In vivo transfer of @entity2247 stripe mosaic hordeivirus ribonucleotides to the 5' terminus of XXXX stripe tenuivirus RNAs.

@entity2247 :: ('4513', 'Species') :: ['Barley', 'Hordeum vulgare'] @entity2829 :: ('4577', 'Species') :: ['maize'] @entity2036 :: ('MESH:D009711', 'Chemical') :: ['nucleotides'] @entity377 :: ('CHEBI:36976', 'Chemical') :: ['nucleotide'] @entity18084 :: ('12327', 'Species') :: ['BSMV', 'barley stripe mosaic virus (BSMV']
@entity2829 :: (4577,Species) :: ['maize']
