UNASSIGNED: We have previously reported decreased expression and activities of lysosomal cathepsins B and L in @entity6 kidney. Relevant morphological changes were observed in proximal tubules, suggesting that these cells are implicated in the early stages of the disease. The aim of the present study was to investigate the mechanisms that lead to these changes. The effects of high @entity413 (HG) and advanced glycation end products (AGEs) on cell viability, lysosomal enzymes and other effectors of cell signaling of cultured kidney cells were studied. HG increased viable mesangial cells (ihMC) in 48  h, while epithelial tubular cells were not affected (LLC-PK1 and MDCK). In contrast, the number of viable cells was markedly decreased, for all cell lines, by AGE-BSA. Concerning lysosomal enzymes, the main @entity462 -protease expressed by these cells was @entity467 , and its concentration was much higher in epithelial than in mesangial cells. Exposure to HG had no effect on the @entity467 activity, but AGE-BSA caused a marked decrease in LLC-PK1, and increased the enzyme activities in the other cell lines. The levels of @entity842 (NO) was increased by AGE-BSA in all cell lines, suggesting oxidative stress, and Western blotting has shown that, among the investigated proteins, @entity467 , @entity3470 and @entity29630 ( @entity29630 ) were the most significantly affected by exposure to AGE-BSA. As @entity3470 induces anabolism and inhibits autophagy, and @entity29630 is a master transcription factor for lysosomal enzymes, it is possible that this pathway plays a role in the inhibition of lysosomal enzymes in proximal tubule cells.

Impact of high XXXX and AGEs on cultured kidney-derived cells. Effects on cell viability, lysosomal enzymes and effectors of cell signaling pathways.

@entity29630 :: ('7942', 'Gene') :: ['transcription factor EB', 'TFEB'] @entity462 :: ('MESH:D003545', 'Chemical') :: ['cysteine'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetic'] @entity467 :: ('1508', 'Gene') :: ['cathepsin B'] @entity3470 :: ('21977(Tax:10090)', 'Gene') :: ['mTOR'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity413 :: (MESH:D005947,Chemical) :: ['glucose']
