UNASSIGNED: Dysfunction of the intestinal epithelial barrier and leakage of luminal antigens and bacteria across the barrier have been linked to various @entity1 diseases. Intestinal permeability is regulated by intercellular structures, termed "tight junction" (Tj), which are disrupted after @entity348 ( @entity348 ). In this study, we investigated radiation-induced alterations in Tj-related proteins in the jejunum, ileum and colon of a non- @entity1 primate (NHP) model. NHPs were total-body irradiated with 6.7 and 7.4 Gy and intestines were procured at day 4, 7 and 12. Radiation exposure was found to induce significant increases in @entity29616 mRNA early (day 4) in all three gut segments and @entity17290 mRNA levels were repressed through day 12. @entity310 was highly induced in the jejunum and colon at early time points, but little induction was found in the ileum. @entity3670 was induced only in the colon on day 4 postirradiation. Unlike the colon and jejunum, the ileum levels of @entity17291 were significantly downregulated through day 12 postirradiation. Western blot analysis revealed increased levels of @entity21486 on day 4 and of @entity17264 on day 7 postirradiation in all three gut segments. @entity5962 was downregulated on day 4 postirradiation in all segments, but remained reduced in the jejunum only until day 12. Taken together, these data suggest that exposure to radiation causes segment-specific alterations in the expression of Tj-related proteins. Interruption of Tjs may be a key factor contributing to injury to the intestinal mucosal barrier and increased intestinal permeability.

Segmental Differences in Radiation-Induced Alterations of Tight Junction-Related Proteins in Non- XXXX Primate Jejunum, Ileum and Colon.

@entity1 :: ('9606', 'Species') :: ['human'] @entity17291 :: ('1366', 'Gene') :: ['claudin-7'] @entity29616 :: ('9071', 'Gene') :: ['claudin-10'] @entity310 :: ('7124', 'Gene') :: ['TNF-alpha'] @entity21486 :: ('9075', 'Gene') :: ['claudin-2'] @entity3670 :: ('9076', 'Gene') :: ['Claudin-1'] @entity5962 :: ('999', 'Gene') :: ['E-cadherin'] @entity17290 :: ('1364', 'Gene') :: ['claudin-4'] @entity348 :: ('MESH:C535338', 'Disease') :: ['total-body irradiation', 'TBI'] @entity17264 :: ('50848', 'Gene') :: ['JAM-1']
@entity1 :: (9606,Species) :: ['Human']
