BACKGROUND: The prognostic values of intratumoral microvessel density (iMVD), @entity5 cell proliferation rate, DNA content (ploidy), and @entity876 protein expression are controversial or have not been well studied in @entity1 with @entity1358 ( @entity1358 ) confined to the kidney. METHODS: A uniform group of 52 clear cell (conventional) RCCs confined to the kidney (classified as T1N0M0 or T2N0M0) were analyzed for iMVD, @entity1758 score, DNA content, S-phase fraction, and @entity876 protein expression by immunohistochemical methods or flow cytometry. iMVD was evaluated in a single area (X200, 1.15 mm2) representative of the highest MVD (neovascular "hot spot") after independently highlighting endothelial cells with antibodies specific for factor VIII-related antigen (F8/86) and @entity2279 (JC/70A). The @entity1758 antibody (Ki-67 antigen) score was used as a marker for the @entity5 cell proliferation rate. DNA content and S-phase fraction were determined by flow cytometry using paraffin embedded tissue. @entity876 expression was assessed using the D07 antibody. RESULTS: The median time of clinical follow-up was > 9 years. Eleven @entity1 died of disease; the median time to @entity204 was 26 months. iMVD counts using antifactor VIII and anti- @entity2279 were tightly correlated (correlation coefficient = 0.89). S-phase fraction was higher in @entity5 than in diploid @entity5 (mean, 12.4% vs. 4.3%; P = 0.01). Using univariate survival analyses, @entity5 size (stage classification pT1 vs. PT2; P = 0.01) and nuclear grade (P = 0.04) were associated with shortened survival. No statistically significant differences in survival were found for iMVD, @entity1758 score, DNA content, S-phase fraction, or @entity876 expression. Only two cases strongly expressed @entity876 protein; both @entity5 were of high nuclear grade. Using multivariate survival analyses, nuclear grade and @entity5 size were the only independent prognostic factors (best model P = 0.002). CONCLUSIONS: In this study, nuclear grade and @entity5 size were found to be independent predictors of survival in locally confined clear cell (conventional) @entity1358 , as has been shown previously for locally confined @entity1358 in general. @entity1758 score, iMVD counts, DNA content, S-phase fraction, and @entity876 expression did not contribute additional prognostic information.

Appraisal of intratumoral microvessel density, @entity1758 score, DNA content, and @entity876 protein expression as prognostic indicators in XXXX with locally confined @entity1358 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors', 'aneuploid tumors'] @entity2279 :: ('5175', 'Gene') :: ['CD31'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity1358 :: ('MESH:D002292', 'Disease') :: ['renal cell carcinoma', 'RCC'] @entity1758 :: ('57534', 'Gene') :: ['MIB-1'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death']
@entity1 :: (9606,Species) :: ['patients']
