Objective To test a novel gene carrier, named @entity10849 nanogel particles ( @entity8681 -NP), for delivering the mircroRNA (miRNA) into Kupffer cells (KCs) for silencing the expression of nuclear transcription factor kB (NF-kB) P65. Methods The capacity of @entity8681 -NPs to carry miRNA was tested by gel electrophoresis. RAW264.7 cell line was transfected with the cationic polymers which mixed together PEI-NP and miRNA. Then, the @entity137 of the RAW264.7 cells was detected by cell counting kit-8 (CCK-8); the cell apoptosis and transfection efficiency were analyzed by flow cytometry combined with @entity10440 - @entity2823 /PI staining; the gene and protein levels of NF-kB P65 were determined respectively by real-time quantitative PCR (qRT-PCR) and Western blotting. The distribution of the cationic polymers in RAW264.7 cells and liver KCs was observed by @entity1579 ( @entity1579 ). The protein level of NF-kB P65 in KCs was detected by immunohistochemistry. Results @entity8681 -NPs were able to completely combine with miRNA at the least mass ratio of 20:1. The highest transfection efficiency of 48 hours was (33.63  1.94)% in vitro. The protein level of NF-kB P65 at 48 hours of @entity8681 -NP/miRNA transfection into RAW264.7 cells was significantly inhibited. @entity1579 showed the cationic polymers in RAW264.7 cytoplasm and only in KCs of liver tissue after transfection. Immunohistochemistry indicated that the protein expression of NF-kB P65 in KCs was significantly lower than that in control group. Conclusion Macrophages and KCs were successfully transfected with the cationic polymers in vitro and in vivo, respectively. The expression of NF-kB P65 was knocked down significantly after transfection.

[Inhibition of nuclear factor kappa B mediated by miRNA plasmid carried by novel XXXX nanogel particles in Kupffer cells of rats].

@entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity10849 :: ('MESH:D011094|MESH:C505405', 'Chemical') :: ['polyethyleneimine'] @entity1579 :: ('MESH:D017096', 'Disease') :: ['TEM', 'transmission electron microscope'] @entity2823 :: ('MESH:D016650', 'Chemical') :: ['FITC'] @entity8681 :: ('CHEBI:53231', 'Chemical') :: ['PEI'] @entity10440 :: ('11747(Tax:10090)', 'Gene') :: ['annexin V']
@entity10849 :: (MESH:D011094|MESH:C505405,Chemical) :: ['polyethyleneimine']
