@entity4353 ( @entity3060 ) gene product, a transcription factor of the basic-helix-loop-helix type, is thought to play a role in the regulation of genes encoding the enzymes necessary for melanogenesis. These include @entity11442 , @entity22919 and @entity22920 . Melanocyte-specific isoform of @entity4353 , @entity3060 -M, is expressed in normal and malignant melanocytes. The presence of two other isoforms of @entity4353 , @entity3060 -A and @entity3060 -H, which differ from @entity3060 -M in the @entity1472 -terminus, was demonstrated also in some non-melanocytic lineages. Here we have analyzed the presence of all three known isoforms of @entity3060 mRNA in a panel of 17 @entity1 @entity320 cell lines by a reverse transcriptase-polymerase chain reaction using isoform-specific primers. While, as expected, the predominant form in @entity320 cell lines was @entity3060 -M, low amounts of @entity3060 -A mRNA was found in almost all @entity320 , as well as in most of 20 @entity5 cell lines of the non-melanocyte origin (lung and @entity694 , @entity421 and @entity521 ). The expression of @entity3060 -H was not detected, with a few exceptions, in the tested cell lines. @entity22284 transcription factor was reported earlier to regulate positively the melanocyte-specific promoter of the @entity3060 gene. We found here that the @entity22284 mRNA was expressed in all @entity320 cell lines, even in those that had repressed the @entity3060 -M and were amelanotic. This suggests that additional factors, besides @entity22284 , are required for the @entity3060 expression. The @entity56292 ( @entity56292 ), a gene originally isolated from melanocytes and containing a strong transcription activation domain, was also found expressed in all @entity320 and most @entity5 cell lines. Together, these data indicate that the @entity3060 -M isoform is the major type of @entity3060 mRNA present in @entity1 @entity320 cell lines and show that the expression of the isoform @entity3060 -A and the @entity56292 is not restricted to malignant melanocytes and occurs in a wide range of @entity5 cell lines.

Expression of genes for @entity4353 isoforms, @entity22284 and @entity56292 , in XXXX @entity320 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity3060 :: ('4286', 'Gene') :: ['MITF'] @entity1472 :: ('CHEBI:46882', 'Chemical') :: ['amino'] @entity4353 :: ('MESH:D008850', 'Disease') :: ['Microphthalmia', 'microphthalmia'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'non-melanocyte tumor'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon carcinomas'] @entity22919 :: ('7217', 'Gene') :: ['TRP-1'] @entity56292 :: ('4435', 'Gene') :: ['MSG1', 'melanocyte-specific gene 1'] @entity421 :: ('MESH:D012516', 'Disease') :: ['osteosarcomas'] @entity22920 :: ('1638', 'Gene') :: ['TRP-2'] @entity521 :: ('MESH:D009447', 'Disease') :: ['neuroblastomas'] @entity11442 :: ('7299', 'Gene') :: ['tyrosinase'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma', 'melanomas'] @entity22284 :: ('5077', 'Gene') :: ['Pax 3', 'Pax3']
@entity1 :: (9606,Species) :: ['human']
