Synthesis of @entity2630 ), a major physiological modulator of plasmin generation, is regulated by growth factors and changes in cell shape. To evaluate the specific relationship between @entity9378 gene expression and cytoarchitecture, serum-free cultures of quiescent @entity35 kidney (NRK) cells were exposed to @entity4620 ( @entity4620 ) at concentrations that disrupt microfilament structure. Treatment with 1-10 microM @entity4620 resulted in an increased 1) incidence of rounded cells, 2) relative @entity9378 mRNA content, and 3) fraction of @entity9378 protein-expressing cells. Abrupt increases in each response were evident at a final concentration of 5 microM @entity4620 . Maximal levels of induced @entity9378 transcripts (18-fold that of control) occurred 4 hours post- @entity4620 addition and declined thereafter but remained elevated (by at least tenfold) for 24 hours. Assessment of the metabolic requirements for @entity4620 -induced @entity9378 expression by using the protein synthesis inhibitors @entity5808 and @entity1880 indicated that @entity9378 transcripts were regulated in a complex manner in response to @entity4620 . The predominant mode of induction reflected secondary (protein synthesis-dependent) metabolic processes, although a minor, albeit significant, primary (protein synthesis-independent) pathway was also evident. @entity9378 mRNA levels in NRK cells maintained in serum- and @entity4620 -free agarose suspension culture were low or undetectable. Relative abundance of @entity9378 transcripts in suspended cells cultured in the presence of @entity4620 , however, closely approximated that of plastic-adherent, @entity4620 -treated cells (13-fold over control). NRK cells in suspension culture with or without @entity4620 were morphologically identical, remaining spherical and unattached. It appears, therefore, that cell rounding alone is not a sufficient stimulus to induce @entity9378 expression in quiescent NRK cells and that perturbation of the actin skeleton as a consequence of @entity4620 treatment is a critical event in the inductive response. A protein @entity669 kinase is likely involved in the @entity4620 -mediated signal-transduction cascade, since induced @entity9378 expression can be down-regulated by @entity670 and @entity11615 but not by @entity11616 or tyrphostin B46.

Cell shape-dependent pathway of XXXX gene expression requires cytoskeletal reorganization.

@entity5808 :: ('MESH:D011691', 'Chemical') :: ['puromycin'] @entity2630 :: ('24617(Tax:10116)', 'Gene') :: ['plasminogen activator inhibitor type-1 (PAI-1'] @entity1880 :: ('MESH:D003513', 'Chemical') :: ['cycloheximide'] @entity4620 :: ('MESH:D003699', 'Disease') :: ['CD', 'cytochalasin D'] @entity670 :: ('MESH:D019833', 'Chemical') :: ['genistein'] @entity9378 :: ('29366(Tax:10116)', 'Gene') :: ['PAI-1'] @entity11615 :: ('MESH:C020754', 'Chemical') :: ['herbimycin A'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine'] @entity11616 :: ('MESH:C058819', 'Chemical') :: ['calphostin C']
@entity9378 :: (29366(Tax:10116),Gene) :: ['plasminogen activator inhibitor type-1']
