OBJECTIVE: To investigate the role of @entity494 /222 in cell proliferation and apoptosis in @entity1 @entity263 cells, and examine the effects of @entity494 /222 on @entity14375 expression. METHODS: @entity263 cells were transfected with @entity494 /222 mimics or inhibitors. Cell proliferation was assessed by @entity98 assay. The expression levels of @entity494 /222 were detected with quantitative real-time PCR. Apoptosis was induced with @entity310 /CHX treatment, and evaluated by @entity5956 staining, @entity2667 (PI) flow cytometric analysis, @entity522 activity measurement, and Western blot analysis. Luciferase activity assay, quantitative real-time PCR, and Western blot were performed to evaluate the effects of @entity494 /222 on @entity14375 expression. RESULTS: Our results showed that @entity494 /222 could promote the proliferation of @entity263 cells, including LNCaP and PC3 cells. After transfection and apoptosis induction, @entity5956 staining and PI flow cytometric assay showed that apoptosis was dramatically decreased in @entity263 cells treated with @entity494 /222 mimics. Moreover, @entity522 activity was dramatically decreased, and the cleaved forms of @entity522 were reduced, in the @entity494 /222 mimic-treated group. On the contrary, @entity494 /222 knockdown sensitized the @entity263 cells to @entity310 /CHX-induced apoptosis. In addition, a negative correlation was observed between the expressions of @entity494 /222 and @entity14375 in @entity263 cells. @entity494 /222 could repress the expression of @entity14375 , which was confirmed by the luciferase reporter assay. CONCLUSION: @entity494 /222 promote cell proliferation and repress apoptosis, through suppressing @entity14375 , in @entity263 cells. Our results provide promising evidence for the miRNA-based therapeutic strategy of @entity263 .

Effects of microRNA-221/222 on cell proliferation and apoptosis in XXXX cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity263 :: ('MESH:D011471', 'Disease') :: ['prostate cancers', 'prostate cancer', 'Prostate cancer'] @entity5956 :: ('MESH:C017807', 'Chemical') :: ['Hoechst 33342'] @entity522 :: ('836', 'Gene') :: ['caspase-3'] @entity2667 :: ('MESH:D011419', 'Chemical') :: ['propidium iodide'] @entity310 :: ('7124', 'Gene') :: ['TNF-a'] @entity14375 :: ('843', 'Gene') :: ['caspase-10'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity494 :: ('407006', 'Gene') :: ['miR-221']
@entity263 :: (MESH:D011471,Disease) :: ['prostate cancer']
