Low-level laser therapy (LLLT) is used in the treatment of chemoradiotherapy- @entity33 ( @entity33 ). In @entity135 , @entity5 cells may lie in the LLLT irradiation field, and LLLT might promote @entity5 progression. We therefore investigated the effect of LLLT on proliferation, cell cycle distribution, and apoptosis in a @entity1 @entity1697 cell line ( @entity1 ), non-malignant epithelial cells (BEAS-2B), and fibroblasts in vitro. The cell lines were subjected to LLLT on three consecutive days for 15 min. Cell proliferation was assessed using the @entity98 assay, cell cycle distribution by flow cytometry and @entity2667 DNA staining, and apoptosis using an @entity4404 - @entity2823 assay. Controls were sham-treated, but not exposed to the laser treatment. LLLT treatment resulted in increased fibroblast proliferation (p < 0.001), whereas decreased cell proliferation was observed after LLLT treatment of BEAS-2B (p = 0.003) and @entity1 cells (p < 0.001). In @entity1 cells, an increased percentage of S-phase cells and decreased percentage of G1-phase cells were observed (p < 0.001). Moreover, a proapoptotic effect of LLLT was observed in @entity1 cells (p = 0.02). LLLT did not exhibit a @entity5 -promoting effect in this in vitro study.

Differential responses of fibroblasts, non-neoplastic epithelial cells, and XXXX cells to low-level laser therapy.

@entity1 :: ('9606', 'Species') :: ['human', 'SCC-25'] @entity135 :: ('MESH:D006258', 'Disease') :: ['head and neck cancer'] @entity1697 :: ('MESH:D009062', 'Disease') :: ['oral carcinoma'] @entity2667 :: ('MESH:D011419', 'Chemical') :: ['propidium-iodide'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity4404 :: ('308', 'Gene') :: ['Annexin V'] @entity33 :: ('MESH:D052016', 'Disease') :: ['or radiotherapy-induced oropharyngeal mucositis', 'ORM'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity2823 :: ('MESH:D016650', 'Chemical') :: ['FITC']
@entity1697 :: (MESH:D009062,Disease) :: ['oral carcinoma']
