1. The correction of @entity520 with @entity4388 remains controversial. Experiments in vitro have suggested possible deleterious effects after alkalinization of the extracellular fluid. Disequilibrium of @entity544 and @entity3157 across cell membranes after alkali administration, leading to the phenomenon of 'paradoxical' intracellular @entity520 , has been held responsible for some of these adverse effects. 2. Changes in intracellular pH in suspensions of leucocytes from healthy volunteers were monitored using a fluorescent intracellular dye. The effect in vitro of increasing extracellular pH with @entity4388 was studied at different @entity4388 concentrations. @entity880 and @entity6137 were added to the extracellular buffer to mimic conditions of @entity520 . 3. The addition of a large bolus of @entity4388 caused intracellular acidification as has been observed previously. The extent of the intracellular @entity520 was dependent on several factors, being most evident at higher starting intracellular pH. When @entity4388 was added as a series of small boluses the reduction in intracellular pH was small. Under conditions of initial @entity520 this was rapidly followed by intracellular alkalinization. 4. Although intracellular acidification occurs after addition of @entity4388 to a suspension of @entity1 leucocytes in vitro, the effect is minimal when the conditions approximate those seen in clinical practice. We suggest that the observed small and transient lowering of intracellular pH is insufficient grounds in itself to abandon the use of @entity4388 in @entity1 @entity520 .

@entity3157 therapy and intracellular XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity4388 :: ('MESH:D017693', 'Chemical') :: ['sodium bicarbonate'] @entity3157 :: ('CHEBI:17544', 'Chemical') :: ['bicarbonate'] @entity520 :: ('MESH:D000138', 'Disease') :: ['acidosis', 'metabolic acidosis'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['Lactic acid'] @entity544 :: ('MESH:D002245', 'Chemical') :: ['carbon dioxide'] @entity6137 :: ('MESH:C029658', 'Chemical') :: ['propionic acid']
@entity520 :: (MESH:D000138,Disease) :: ['acidosis']
