We identified cDNA encoding a functional @entity14950 (growth hormone secretagogue-receptor 1a (GHS-R1a)) in a urodele amphibian, the Japanese fire belly newt (Cynops pyrrhogaster). Two functional receptor proteins, composed of 378- and 362-amino acids, were deduced from the identified cDNA because two candidate initiation methionine sites were found. The long-chain receptor protein shared 80%, 69%, and 59% identities with the bullfrog GHS-R1a, @entity1 GHS-R1a and tilapia GHS-R1a-like receptor, respectively. Phylogenetic analysis suggested that the newt receptor is grouped to the clade of the tetrapod homologs, and very closed to anuran amphibians. In functional analyses, homologous newt @entity9315 , heterologous bullfrog and @entity35 @entity9315 , and a GHS-R1a agonist, GHRP-6 increased intracellular Ca(2+) concentration in @entity1 embryonic kidney (HEK) 293 cells stably expressed newt GHS-R1a. The responsiveness was much greater in the short-chain receptor than in the long-chain receptor. Both receptors preferred to bind Ser(3)- @entity9315 including newt and @entity35 @entity9315 than Thr(3)- @entity9315 with bullfrog @entity9315 . GHRP-6 has a similar affinity to bullfrog @entity9315 . GHS-R1a mRNA was expressed in the brain, pituitary, intestine, pancreas, testis and fat body with high level, and eyes, heart, stomach, liver, gall bladder, kidney and dorsal skin with low level. In a fasting experiment, gene expression of GHS-R1a in the brain and pituitary increased 4days after fasting, and the increased level decreased to the initial level 2weeks after fasting. These changes are consistent with the change in @entity9315 mRNA. In contrast, expression of @entity9315 and GHS-R1a mRNA in the stomach decreased on day 4 after fasting, and increased 2weeks after fasting. These results indicate that @entity9315 and its receptor system are present and altered by energy states in this newt.

XXXX in Japanese fire belly newt, Cynops pyrrhogaster.

@entity1 :: ('9606', 'Species') :: ['human'] @entity14950 :: ('2693', 'Gene') :: ['ghrelin receptor'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity9315 :: ('59301(Tax:10116)', 'Gene') :: ['ghrelin']
@entity14950 :: (2693,Gene) :: ['Ghrelin receptor']
