It is unclear how mechanical stress influences bone cells. Mechanical stress causes @entity1642 ( @entity1642 ) in the bone. Osteoblast lineage cells are thought to sense @entity1642 and regulate bone remodeling. We therefore investigated the effects of @entity1642 on @entity1 osteoblast-like @entity421 cells: SaOS-2 cells in vitro. The conditioned medium of the SaOS-2 cells after 24 h of @entity1642 (24 h- @entity1642 CM) showed such @entity2527 phenotype inductions as significantly increasing the number of @entity5272 -resistant acid phosphatase ( @entity62674 ) positive multinuclear cells in @entity35 bone marrow cells and @entity62674 -positive cells in @entity1 preosteoclastic cells: FLG 29.1 cells. An enzyme-linked immunosorbent assay showed @entity4382 ( @entity4382 ) protein to increase 7-fold in the 24 h- @entity1642 CM. A Northern analysis showed that @entity4382 mRNA increased 4-fold in the SaOS-2 cells after 6 h- @entity1642 ; however, no @entity650 mRNA expression was detected. Furthermore, the anti-human @entity4382 antibody significantly neutralized the @entity2527 phenotype induction of the 24 h- @entity1642 CM. The @entity4382 mRNA up-regulation in SaOS-2 cells by the 6 h- @entity1642 was not inhibited by the anti-human @entity2533 antibody, but it was significantly inhibited by @entity3352 . An enzymeimmunoassay showed @entity1421 to increase 7-fold in the 1 h- @entity1642 CM. These findings thus suggest that @entity1642 induces osteoblasts to produce @entity4382 (mediated by @entity2918 ) and thus stimulates bone remodeling.

Fluid shear stress increases @entity4382 expression in XXXX osteoblast-like cells: its role in osteoclast induction.

@entity1 :: ('9606', 'Species') :: ['human'] @entity5272 :: ('MESH:C029768', 'Chemical') :: ['tartrate'] @entity650 :: ('3569', 'Gene') :: ['IL-6'] @entity62674 :: ('25732(Tax:10116)', 'Gene') :: ['TRAP'] @entity4382 :: ('3589', 'Gene') :: ['IL-11', 'interleukin-11'] @entity1421 :: ('MESH:D015232', 'Chemical') :: ['prostaglandin E2'] @entity2918 :: ('MESH:D011453', 'Chemical') :: ['prostaglandins'] @entity421 :: ('MESH:D012516', 'Disease') :: ['osteosarcoma'] @entity2533 :: ('7040', 'Gene') :: ['transforming growth factor-beta1'] @entity2527 :: ('MESH:D001862', 'Disease') :: ['osteoclastic'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity3352 :: ('MESH:D007213', 'Chemical') :: ['indomethacin'] @entity1642 :: ('MESH:D015775', 'Disease') :: ['fluid shear stress', 'FSS']
@entity1 :: (9606,Species) :: ['human']
