UNASSIGNED: Peritoneal fluid dwell impacts the peritoneum by creating an abnormal physiological microenvironment. Little is known about the precise effects of fluid dwell on the peritoneum, and no adequate in vitro models to analyze the impact of fluid dwell have been established. In this study, we developed a peritoneal fluid dwell model combined with an artificial peritoneal cavity and fluid stirring generation system to clarify the effects of different dwelling solutions on the peritoneum over time. To replicate the peritoneal cavity, we devised a reconstructed peritoneal cavity utilizing a mesothelial layer, endothelial layer, and collagen membrane chamber. The reconstructed peritoneal cavity was infused with Dulbecco's modified Eagle's medium, saline, lactated Ringer's solution or peritoneal dialysis solution with repeated 4-h dwells for 10 or 20 consecutive days. The above-described solutions induced epithelial-mesenchymal transition (EMT) and @entity1373 of mesothelial cells. All solution types modulated @entity842 synthase activities in mesothelial and endothelial cells and @entity842 concentrations in dwelling solutions. Inhibition of @entity842 synthase activity acted synergistically on mesothelial EMT and @entity1373 . The present findings suggest that solutions infused into the peritoneal cavity are likely to affect @entity842 production in the peritoneum and promote peritoneal @entity82 . Our newly devised peritoneal cavity model should be a promising tool for understanding peritoneal cellular kinetics and homeostasis.

Fluid dwell impact induces peritoneal XXXX in the peritoneal cavity reconstructed in vitro.

@entity1373 :: ('MESH:D006965', 'Disease') :: ['hyperplasia'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide']
@entity82 :: (MESH:D005355,Disease) :: ['fibrosis']
