PURPOSE: In the dome of the bladder of the @entity1707 muscarinic receptor stimulation has been shown to release a factor from the urothelium that exerts an inhibitory effect on the underlying smooth muscle. We examined whether the urothelium in the trigone of the bladder also releases this factor, identified which receptors stimulate its release and investigated possible cross-talk among these receptor systems in the trigone. MATERIALS AND METHODS: Paired longitudinal strips of @entity1707 bladder were isolated, the urothelium was removed from 1 strip per pair and tissues were set up in gassed Krebs solution at 37C. Cumulative concentration-response curves to @entity5402 , @entity2647 or @entity112 were constructed. In some tissues a second @entity2647 curve was constructed in the presence of 1 microM. @entity5402 or 1 microM. @entity112 . In a further group of tissues the second @entity2647 curve was constructed in the presence of 1 microM. @entity5402 and 1 microM. @entity3897 or 1 microM. @entity112 and 100 nM. @entity8765 . RESULTS: In the presence of an intact urothelium contractile responses to @entity5402 and @entity112 but not to @entity2647 were @entity308 . In the presence of 1 microM. @entity5402 or 1 microM. @entity112 the responses of intact urothelium strips to @entity2647 were significantly @entity308 . This effect was absent in the presence of @entity3897 and @entity8765 , respectively. CONCLUSIONS: @entity5402 and @entity112 induce the release of a urothelium derived inhibitory factor in the bladder trigone. The factor appears to mediate cross-talk between these systems and the alpha-adrenoceptor system in this region of the bladder.

Urothelium derived inhibitory factor and cross-talk among receptors in the trigone of the bladder of the XXXX .

@entity1707 :: ('9823', 'Species') :: ['pig'] @entity308 :: ('MESH:D003866', 'Disease') :: ['depressed'] @entity8765 :: ('MESH:D011738', 'Chemical') :: ['mepyramine'] @entity2647 :: ('MESH:D010656', 'Chemical') :: ['phenylephrine'] @entity3897 :: ('MESH:D001285', 'Chemical') :: ['atropine'] @entity112 :: ('MESH:D006632', 'Chemical') :: ['histamine'] @entity5402 :: ('MESH:D002217', 'Chemical') :: ['carbachol', 'Carbachol']
@entity1707 :: (9823,Species) :: ['pig']
