BACKGROUND: Increased numbers of eosinophils in the airways is characteristic of @entity565 . However, it remains unclear whether airway eosinophils enhance or reduce the release of neuropeptides in the airways in vivo. This study was conducted to elucidate the influence of airway eosinophil accumulation on the ultrasonically nebulized distilled water (UNDW)-induced bronchoconstriction in our newly developed animal model, which is mediated by sensory neuropeptides. METHODS: @entity840 were transnasally treated with 100 mg/kg of platelet activating factor ( @entity5567 ), or vehicle, twice a week for 3 weeks. We then conducted three experiments. In the first, UNDW was inhaled 20 min after aerosolized antigen challenge, and bronchoalveolar lavage (BAL) was performed in @entity5567 -treated and passively sensitized animals. In the second, @entity5567 -treated animals were exposed for 20 s to ascending doses of @entity2710 at intervals of 5 min In the third, passively sensitized animals were administered selective NK1 antagonist, @entity13631 , selective @entity10125 antagonist, @entity21313 , or vehicle, intravenously 5 min before UNDW-induced bronchoconstriction. RESULTS: The proportion of eosinophils in BAL fluid was significantly increased in @entity840 treated with @entity5567 , compared with the vehicle. The @entity5567 treatment did not affect antigen-induced immediate @entity748 response, UNDW-induced bronchoconstriction, or bronchial responsiveness to inhaled @entity2710 . @entity13631 , but not @entity21313 , inhibited the UNDW-induced bronchoconstriction. CONCLUSION: We conclude that eosinophils accumulated in the airways, caused by repeated intranasal administration of @entity5567 , does not affect the release of substance P induced by UNDW inhalation, or the action of released substance P in vivo.

Airway eosinophil accumulation on sensory neuropeptide release in a XXXX model of distilled-water-induced bronchoconstriction.

@entity748 :: ('MESH:D013224', 'Disease') :: ['asthmatic'] @entity13631 :: ('MESH:C085215', 'Chemical') :: ['SR 140333'] @entity565 :: ('MESH:D001249', 'Disease') :: ['asthma'] @entity840 :: ('10141', 'Species') :: ['Guinea pigs', 'guinea pigs'] @entity21313 :: ('MESH:C073839', 'Chemical') :: ['SR 48968'] @entity10125 :: ('MESH:C006007', 'Chemical') :: ['NK2'] @entity5567 :: ('CHEBI:52450', 'Chemical') :: ['PAF'] @entity2710 :: ('MESH:D016210', 'Chemical') :: ['methacholine']
@entity840 :: (10141,Species) :: ['guinea pig']
