Exposure to high-dose radiation results in detrimental effects on survival. The effects of combined @entity130 , such as radiation in combination with @entity548 , the typical injury of victims exposed to a radiation blast, on survival and hematopoietic effects have yet to be understood. The purpose of this study was to evaluate the effects of @entity1161 ( @entity1161 ) combined with @entity548 (i.e., combined injury, CI) on survival and hematopoietic effects, and to investigate whether @entity548 ( @entity548 ) enhanced @entity1161 -induced mortality and @entity1583 . Male CD2F1 @entity19 (10 weeks old) were given one single exposure of y- radiation (60Co) at various doses (0.6 Gy/min). Within 2 hr after @entity1161 , animals under anesthesia were bled 0% (Sham) or 20% ( @entity548 ) of total blood volume via the submandibular vein. In these @entity19 , @entity548 reduced the LD50/30 for 30-day survival from 9.1 Gy ( @entity1161 ) to 8.75 Gy (CI) with a DMF of 1.046. @entity1161 resulted in @entity1431 , @entity882 , erythropenia, and bone marrow cell depletion, but decreased the @entity185 activation response. @entity1161 increased @entity635 , @entity777 , @entity636 , and @entity637 concentrations in serum, bone marrow, ileum, spleen, and kidney. Some of these adverse alterations were magnified by CI. @entity14190 production was increased in kidney and blood more after CI than @entity1161 . Furthermore, CI altered the global miRNAs expression in kidney and the ingenuity pathway analysis showed that miRNAs viz., @entity34638 , @entity29036 and miR-29b that were associated with @entity1210 and @entity32 . This study provides preliminary evidence that non-lethal @entity548 exacerbates @entity1161 -induced mortality and cell losses associated with high-dose y-radiation. We identified some of the initial changes occurring due to CI which may have facilitated in worsening the injury and hampering the recovery of animals ultimately resulting in higher mortality.

@entity548 Exacerbates Radiation Effects on Survival, @entity1431 , XXXX , Erythropenia, Bone Marrow Cell Depletion and Hematopoiesis, and Inflammation-Associated microRNAs Expression in Kidney.

@entity29036 :: ('723836(Tax:10090)', 'Gene') :: ['miR-30e'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity14190 :: ('13856(Tax:10090)', 'Gene') :: ['Erythropoietin'] @entity34638 :: ('75202(Tax:10090)', 'Gene') :: ['let-7e'] @entity548 :: ('MESH:D006470', 'Disease') :: ['hemorrhage', 'Hemo'] @entity882 :: ('MESH:D013921', 'Disease') :: ['thrombopenia'] @entity1161 :: ('MESH:D011832', 'Disease') :: ['RI', 'radiation injury'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity635 :: ('16176(Tax:10090)', 'Gene') :: ['IL-1b'] @entity185 :: ('12367(Tax:10090)', 'Gene') :: ['caspase-3'] @entity777 :: ('16193(Tax:10090)', 'Gene') :: ['IL-6'] @entity1583 :: ('MESH:D019337', 'Disease') :: ['hematopoietic syndrome'] @entity1431 :: ('MESH:D007970', 'Disease') :: ['leukocytopenia'] @entity636 :: ('16171(Tax:10090)', 'Gene') :: ['IL-17A'] @entity637 :: ('21926(Tax:10090)', 'Gene') :: ['TNF-a'] @entity130 :: ('MESH:D014947', 'Disease') :: ['trauma'] @entity1210 :: ('MESH:C536227', 'Disease') :: ['hematopoiesis']
@entity882 :: (MESH:D013921,Disease) :: ['Thrombopenia']
