BACKGROUND: @entity2533 is involved in the pathogenesis of both drug-induced @entity8032 and @entity29883 . Ribozymes enzymatically cleave target mRNAs and are expected to be utilized as the basis of novel nucleic acid-based therapies. We designed a chimeric DNA-RNA ribozyme targeting @entity2533 mRNA and examined its effect on growth of gingival fibroblasts in culture. METHODS: Chimeric DNA-RNA hammerhead ribozyme with sequence complementary to the loop structure of @entity1 @entity2533 mRNA was used. We evaluated transfer of the chimeric ribozyme by hemagglutinating virus of Japan (HVJ)-envelope into cultured @entity1 gingival fibroblasts in vitro and @entity35 gingival tissues in vivo. We then examined effects of the chimeric ribozyme to @entity2533 on proliferation and DNA synthesis in @entity1 gingival fibroblasts. We also examined effects of the chimeric ribozyme to @entity2533 on expression of @entity2533 , type IV collagens, and fibronectin mRNAs and expression of @entity2533 protein in @entity1 gingival fibroblasts. RESULTS: Chimeric ribozyme was sufficiently distributed into @entity1 fibroblasts in vitro and @entity35 gingivae in vivo. Chimeric ribozyme to @entity2533 significantly inhibited expression of @entity2533 , type IV collagen, and fibronectin mRNAs and @entity2533 protein in @entity1 gingival fibroblasts. Mismatch ribozyme had no effect on expression of these molecules. Chimeric ribozyme to @entity2533 also significantly inhibited proliferation and DNA synthesis in gingival fibroblasts. CONCLUSION: Chimeric DNA-RNA ribozyme targeting @entity2533 may be a useful gene therapy agent for treatment of @entity7138 .

Inhibition of growth of XXXX gingival fibroblasts by chimeric DNA-RNA hammerhead ribozyme targeting transforming growth factor-beta 1.

@entity1 :: ('9606', 'Species') :: ['human'] @entity2533 :: ('7040', 'Gene') :: ['TGF-beta1', 'Transforming growth factor (TGF)-beta1'] @entity7138 :: ('MESH:D005885', 'Disease') :: ['gingival hyperplasia'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity29883 :: ('MESH:C562884', 'Disease') :: ['hereditary gingival fibromatosis'] @entity8032 :: ('MESH:D019214', 'Disease') :: ['gingival overgrowth']
@entity1 :: (9606,Species) :: ['human']
