Since dendritic cells (DCs) play a critical role in establishing antigen-specific adaptive immune responses, in the past several years, therapeutic strategies using genetically modified DCs against @entity5 and @entity166 have attracted increasing attention. In the present study, we demonstrated that RGD fiber-mutant adenovirus vector (AdRGD) exhibited markedly superior gene transduction efficiency in @entity19 bone marrow-derived DCs (mBM-DCs) compared to conventional adenovirus vector (Ad). Likewise, this vector exhibited superior major histocompatibility complex class I-restricted presentation of antigen derived from the delivered gene in mBM-DCs. In order to investigate the effect of @entity9504 on the DC-differentiation process (maturation), we used three types of AdRGD and three conventional Ad to transduce mBM-DCs. These vectors carried either no transgene, LacZ gene, or @entity33933 gene. Infection by any of the Ad vectors enhanced the expression of MHC class II molecules in mBM-DCs. @entity8271 , @entity11787 , and @entity14636 expression and IL-12 production were more efficient in AdRGD-infected mBM-DCs than in conventional Ad-infected cells. Contrary to our expectations, endocytotic activity of mBM-DCs decreased only slightly upon @entity9504 , whereas antigen uptake by lipopolysaccharide (LPS)-driven mature mBM-DCs was significantly impaired. However, our reverse transcription-polymerase chain reaction analysis revealed that @entity9504 resulted in the upregulation of the chemokine receptor @entity13386 and downregulation of @entity14012 in mBM-DCs and LPS-stimulated cells. We, therefore, concluded that @entity9504 directly influenced DC-maturation, although the effects were milder than under LPS-stimulation. In addition, this change in the immunologic properties of DCs resulted primarily from an increase in the number of Ad-particles capable of invading the cells rather than from the expression of foreign genes. @entity281 caused greater induction of maturation than conventional @entity9504 , irrespective of the type of transgene inserted.

Gene transduction efficiency and maturation status in XXXX bone marrow-derived dendritic cells infected with conventional or RGD fiber-mutant adenovirus vectors.

@entity9504 :: ('MESH:D000257', 'Disease') :: ['Ad-infection'] @entity13386 :: ('12775(Tax:10090)', 'Gene') :: ['CCR7'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity8271 :: ('12519(Tax:10090)', 'Gene') :: ['CD80'] @entity33933 :: ('20431(Tax:10090)', 'Gene') :: ['gp100'] @entity14636 :: ('21939(Tax:10090)', 'Gene') :: ['CD40'] @entity11787 :: ('12524(Tax:10090)', 'Gene') :: ['CD86'] @entity281 :: ('MESH:D007239', 'Disease') :: ['AdRGD-infection'] @entity14012 :: ('12458(Tax:10090)', 'Gene') :: ['CCR6'] @entity166 :: ('MESH:D003141', 'Disease') :: ['infectious diseases']
@entity19 :: (10090,Species) :: ['mouse']
