The 5' upstream region (about 3.1kb) of the cellulose synthase operon (bcs operon) has been isolated by cloning from @entity20196 BPR 2001. The expression level of the upstream region was determined using @entity969 synthase cDNA as a reporter gene in the shuttle vector pSA19. The expression occurred with the 1.1-kb upstream sequence from the ATG start codon of the bcs operon but not with the 241-bp upstream sequence in A. xylinum, although neither the 1.1-kb nor the 241-bp upstream sequence caused any expression as a promoter in @entity526 . The level of expression with the 1. 1-kb upstream sequence in A. aceti was 75% of that in A. xylinum. These results suggest that the upstream region functions as a specific promoter for the Acetobacter genus. The expression was reduced by the introduction of the 241-bp upstream region between the lac promoter and the reporter gene in @entity526 and was not detected in A. xylinum. This suggests that the short upstream region composed of 241bp contains the site(s) which causes a negative regulation on the transcription for bcs operon. The production of recombinant protein with the ribosome-binding site (RBS) of A. xylinum obtained from the bcs operon, was reduced to about half in @entity526 , and that with the site of the lac promoter was also reduced to about half in A. xylinum. This shows that a species-specific predominance occurs during interaction between mRNA and 16S rRNA in the RBS between A. xylinum and @entity526 .

Control of expression by the cellulose synthase (bcsA) promoter region from XXXX BPR 2001.

@entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity526 :: ('562', 'Species') :: ['Escherichia coli', 'E. coli'] @entity20196 :: ('28448', 'Species') :: ['Acetobacter xylinum strain']
@entity20196 :: (28448,Species) :: ['Acetobacter xylinum']
