OBJECTIVE: To identify unique epigenetic signature in @entity5 stem cells (CSCs) in @entity213 ( @entity213 ). STUDY DESIGN: Molecular and microarray studies. SETTING: Tertiary referral center. SUBJECTS AND METHODS: Head and neck CSCs were isolated in @entity213 cells by @entity1759 staining and flow cytometry sorting. CSCs with highest @entity1759 expression ( @entity1759 (hi)) and non-stem cells (non-SCs) with lowest @entity1759 expression ( @entity1759 (low)) were then characterized for @entity3308 gene expression and their responses to chemotherapeutic agents, followed by high-throughput epigenetic profiling using the Illumina BeadChip Array, targeting 28,544 CpG sites covering more than 14,956 genes. RESULTS: @entity1759 (hi) CSCs expressed higher levels of stem cell markers and were more resistant to chemotherapeutic agents as compared to @entity1759 (low) non-SCs. By DNA methylation microarray analysis, 17 hypomethylated and 9 hypermethylated genes were identified in @entity1759 (hi) CSCs as compared to non-SCs in most @entity213 cell lines. Cluster analysis using these 26 genes showed that @entity1759 (hi) CSCs were epigenetically distinct from the @entity1759 (low) non-SCs in all 5 @entity213 cell lines. CONCLUSION: A unique epigenetic profile consisting of 17 hypomethylated and 9 hypermethylated genes was seen in @entity213 CSCs. These genes may be critically required in maintaining the @entity3308 or pluripotency of CSCs and may represent novel molecular targets for anticancer therapies aimed at eradicating CSCs in @entity213 .

Distinct epigenetic profiling in XXXX stem cells.

@entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity1759 :: ('960', 'Gene') :: ['CD44'] @entity3308 :: ('MESH:D020295', 'Disease') :: ['stemness'] @entity213 :: ('MESH:C535575', 'Disease') :: ['head and neck squamous cell carcinoma', 'HNSCC']
@entity213 :: (MESH:C535575,Disease) :: ['head and neck squamous cell carcinoma']
