PURPOSE: To extract the Melaleuca sp. oil and to assess its in vitro inhibitory effect against @entity794 isolates obtained from @entity6250 and resistant to several antibiotics. METHODS: A total of 14 test-tubes containing Mueller-Hinton broth were used to determine the @entity6251 ( @entity6251 ). The following concentrations of the Melaleuca sp. oil were added to the first 11 tubes: 8; 4; 2; 1; 0.5; 0.2; 0.1; 0.05; 0.025; 0.0125 and 0.00625%. The 12th and 13th tubes, with and without oil, were used as the positive and negative controls, respectively. The experimental study was carried out in triplicate at 37  C for 18 hours. The @entity6252 ( @entity6252 ), able of killing all the microorganisms, was also determined. Two @entity794 isolates were obtained from @entity6250 of female @entity1 and the identification of the microorganisms ( @entity794 ) and the test for susceptibility to the antimicrobial agents were carried out by automation using the apparatus MicroScan(r). After identification, the isolates were preserved in liquid Trypticase Soy medium, and inoculated for determination of the @entity6251 and @entity6252 . RESULTS: The @entity6251 was 0.2% and the @entity6252 was 0.4%. CONCLUSION: The Melaleuca sp. oil showed antimicrobial properties in vitro against strains isolated from @entity6250 which were resistant to multiple antibiotics.

Antimicrobial activity of Melaleuca sp. oil against clinical isolates of antibiotics resistant XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity794 :: ('1280', 'Species') :: ['Staphylococcus aureus', 'S. aureus'] @entity6250 :: ('MESH:D038061', 'Disease') :: ['lower limb wounds'] @entity6251 :: ('MESH:C567712', 'Disease') :: ['MIC', 'Minimum Inhibitory Concentration'] @entity6252 :: ('MESH:D010585', 'Disease') :: ['Minimum Bactericidal Concentration', 'MBC']
@entity794 :: (1280,Species) :: ['Staphylococcus aureus']
