@entity513 in situ of the breast (DCIS) is surrounded by a layer of myoepithelial cells. Our previous studies have suggested that these myoepithelial cells exert paracrine @entity5 -suppressive effects on invasion of @entity0 cells. @entity558 ( @entity558 ), concentrated 10-100x of @entity37007 -1, @entity37007 -3, and @entity37007 -4, @entity1 myoepithelial cell lines, block Matrigel invasion of a series of @entity189 cell lines. Immunoprecipitation of @entity24208 , a recently described serpin, from these @entity558 abolishes this anti-invasive effect. Both @entity558 and @entity24208 -immunoprecipitated @entity558 , however, exert equal antiproliferative effects on a series of ER+ and ER- cell lines including MCF-7, T47D, MDA-MB-231, and MDA-MB-468. These antiproliferative effects are characterized by induction of a G2/M arrest, a twofold increase in @entity994 ( @entity994 / @entity994 ) transcription and expression, and a threefold increase in apoptosis in the @entity0 lines examined. The antiproliferative effects mediated by myoepithelial cell @entity558 do not manifest themselves in an autocrine manner, are not mediated by @entity2533 , nor involve ER- or @entity876 -dependent pathways. Neither the antiproliferative nor the anti-invasive effects of myoepithelial cell @entity558 is observed with nonmyoepithelial cell @entity558 . The in vitro observations of our present study may have relevance in explaining the increased degree of apoptosis exhibited by DCIS cells in vivo. Our findings illustrate another way myoepithelial cells function as natural paracrine @entity5 suppressors.

The @entity1 myoepithelial cell exerts antiproliferative effects on XXXX cells characterized by p21WAF1/ @entity994 induction, G2/M arrest, and apoptosis.

@entity1 :: ('9606', 'Species') :: ['human'] @entity558 :: ('MESH:C536528', 'Disease') :: ['CM', 'Conditioned medium'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast carcinoma'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity37007 :: ('MESH:C537627', 'Disease') :: ['HMS'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinoma'] @entity513 :: ('MESH:D044584', 'Disease') :: ['Ductal carcinoma'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity24208 :: ('5268', 'Gene') :: ['maspin'] @entity2533 :: ('7040', 'Gene') :: ['TGF-beta1'] @entity994 :: ('1026', 'Gene') :: ['CIP1', 'p21', 'WAF1']
@entity0 :: (MESH:D001943,Disease) :: ['breast carcinoma']
