PURPOSE: The proteomic profile of vitreous from second-trimester @entity1 embryos and young adults was characterized using mass spectrometry and analyzed for changes in protein levels that may relate to structural changes occurring during this time. This vitreous proteome was compared to previous reports to confirm proteins already identified and reveal novel ones. METHODS: Vitreous from 17 @entity1 embryos aged 14 to 20 @entity6677 ( @entity6677 ) and from a 12-, a 14-, a 15-, and a 28-year-old was individually analyzed using tandem mass spectrometry-based proteomics. Peptide spectral count associations with embryonic age were assessed using a general linear model of fold changes and Spearman's rank correlation. Differences between embryonic and young adult vitreous proteomes were also compared. Immunohistochemistry was used to evaluate three proteins in five additional fetal (10-18 @entity6677 ) @entity1 eyes. RESULTS: There were 1217 proteins identified in fetal and young adult @entity1 vitreous, 206 after quantile normalization and variance filtering. In embryos, the peptide counts of 37 proteins changed significantly from 14 to 20 @entity6677 : 75.7% increased, 24.3% decreased. Immunohistochemistry confirmed the absence of clusterin and cadherin in 10 and 14 @entity6677 eyes and their presence at 18 @entity6677 . Comparing embryonic to young adult vitreous, 47 proteins were significantly higher or lower. A total of 768 proteins not previously identified in the literature are presented. CONCLUSIONS: Proteins previously unreported in the @entity1 vitreous were identified. The @entity1 vitreous proteome undergoes significant changes during embryogenesis and young adulthood. A number of protein levels change considerably during the second trimester, with the majority decreasing.

Proteomic Analysis of Embryonic and Young XXXX Vitreous.

@entity1 :: ('9606', 'Species') :: ['human'] @entity6677 :: ('MESH:D016640', 'Disease') :: ['WG', 'weeks gestation']
@entity1 :: (9606,Species) :: ['Human']
