BACKGROUND: We previously showed that mitochondrial respiratory function is abnormal in @entity235 with chronic @entity712 ( @entity712 ). Mitochondrial permeability transition pores ( @entity5212 ) can affect mitochondrial inner membrane potential (DeltaPsim) and mitochondrial function in normal cardiomyocytes. The potential impact of @entity5212 on DeltaPsim and mitochondrial respiratory function in @entity712 has not yet been determined. We tested the hypothesis that @entity205 , a potent blocker of the @entity5212 , can improve mitochondrial function in @entity712 . METHODS: Cardiomyocytes were isolated from the left ventricular myocardium of 7 @entity235 with @entity712 produced by intracoronary microembolizations and from 7 normal @entity235 . Cardiomyocytes were treated for 24 hours with @entity205 . DeltaPsim, cytochrome c oxidase protein expression, mitochondrial cytochrome c oxidase-dependent respiration (CDOR) and @entity855 synthesis were measured. RESULTS: DeltaPsim, protein expression of cytochrome c oxidase, CDOR and the rate of @entity855 synthesis were decreased in @entity712 compared to normal controls. Inhibition of @entity5212 in failing cardiomyocytes with low dose of @entity205 (0.2 microM) increased DeltaPsim, preserved expression of cytochrome c oxidase, improved CDOR and the rate of @entity855 synthesis. CONCLUSION: @entity5212 opening contributes to the loss of mitochondrial function observed in the failing heart. Inhibition of @entity5212 opening represents a potential therapeutic target for the treatment of @entity712 .

Inhibition of mitochondrial permeability transition pores by @entity205 improves cytochrome C oxidase function and increases rate of XXXX synthesis in failing cardiomyocytes.

@entity5212 :: ('MESH:D015632', 'Chemical') :: ['MPTP'] @entity712 :: ('MESH:D006333', 'Disease') :: ['HF', 'heart failure'] @entity235 :: ('9615', 'Species') :: ['dogs'] @entity205 :: ('MESH:D016572', 'Chemical') :: ['cyclosporine A'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity855 :: (MESH:D000255,Chemical) :: ['ATP']
