UNASSIGNED: The @entity18935 cytoskeleton is highly conserved among eukaryotes and is essential for cellular processes regulating growth and differentiation. In fungi, filamentous @entity18935 (F- @entity18935 ) orchestrates hyphal tip structure and extension via organization of exocytic and endocytic processes at the hyphal tip. Although highly conserved, there are key differences among actins of fungal species as well as between @entity1 and fungal actins. For example, the F- @entity18935 stabilizing molecules, @entity6286 and @entity13298 , bind to @entity18935 structures in @entity375 and @entity1 cells, whereas @entity6286 does not bind @entity18935 structures of @entity54 . These discrepancies suggest structural differences between @entity54 @entity18935 filaments and those of @entity1 and @entity375 cells. Additionally, fungal @entity18935 kinetics are much faster than those of @entity1 , displaying 5-fold faster nucleation and 40-fold faster @entity4334 exchange rates. Limited published studies suggest that these faster @entity18935 kinetics are required for normal growth and morphogenesis of @entity375 cells. In the current work, we show that replacement of @entity54 @entity18935 with @entity375 @entity18935 generates a morphologically normal strain, suggesting that @entity54 @entity18935 kinetics are similar to those of @entity375 . In contrast to wild type A. fumigatus, F- @entity18935 in this strain binds @entity6286 , and pharmacological stabilization of these @entity18935 structures with @entity13298 inhibits germination and alters morphogenesis in a dose-dependent manner. We also show that @entity1 b- @entity18935 cannot support @entity54 viability, even though the @entity3689 sequences of @entity1 and @entity54 actins are 89.3% identical. Our findings show that minor differences in @entity18935 protein sequence account for loss of @entity6286 and @entity13298 sensitivity in @entity54 species.

Differential Support of @entity54 Morphogenesis by XXXX and @entity1 Actins.

@entity1 :: ('9606', 'Species') :: ['mammalian', 'humans', 'human'] @entity13298 :: ('MESH:C057531', 'Chemical') :: ['jasplakinolide'] @entity6286 :: ('MESH:D010590', 'Chemical') :: ['phalloidin'] @entity18935 :: ('850504(Tax:4932)', 'Gene') :: ['actin'] @entity54 :: ('746128', 'Species') :: ['Aspergillus'] @entity4334 :: ('D009711', 'Chemical') :: ['nucleotide'] @entity375 :: ('4932', 'Species') :: ['yeast'] @entity3689 :: ('D000596', 'Chemical') :: ['amino acid']
@entity375 :: (4932,Species) :: ['Yeast']
