UNASSIGNED: @entity1 lung mast cells (HLMCs) play a central role in @entity565 pathogenesis through their relocation to the @entity3960 ( @entity3960 ) bundles. @entity2162 ( @entity2162 )-agonists are used to relieve bronchoconstriction in @entity565 , but may reduce @entity565 control, particularly when used as monotherapy. We hypothesized that HLMC and @entity1 @entity3960 cell (HASMC) responsiveness to @entity2162 agonists would be attenuated when HLMCs are in contact with HASMCs. Cells were cultured in the presence of the short-acting b2-agonist @entity3045 , and the long-acting b2-agonists @entity6410 and @entity69013 . Constitutive and @entity10685 -dependent HLMC @entity112 release, HASMC contraction, and @entity2162 phosphorylation at Tyr(350) were assessed. Constitutive HLMC @entity112 release was increased in HLMC-HASMC coculture and this was enhanced by @entity2162 agonists. Inhibition of @entity10685 -dependent HLMC mediator release by b2-agonists was greatly reduced in HLMC-HASMC coculture. These effects were reversed by neutralization of @entity6826 ( @entity6826 ) or @entity23690 ( @entity23690 ). @entity2162 agonists did not prevent HASMC contraction when HLMCs were present, but this was reversed by @entity5023 . @entity2162 phosphorylation at Tyr(350) occurred within 5 min in both HLMCs and HASMCs when the cells were cocultured, and was inhibited by neutralizing @entity6826 or @entity23690 . HLMC interactions with HASMCs via @entity23690 and Kit inhibit the potentially beneficial effects of @entity2162 agonists on these cells via phosphorylation of the @entity2162 . These results may explain the potentially adverse effects of b2-ARs agonists when used for @entity565 therapy. Targeting @entity6826 and @entity23690 may enhance @entity2162 efficacy, particularly in @entity228 -resistant @entity1 .

Bidirectional Counterregulation of XXXX Lung Mast Cell and Airway Smooth Muscle b2 Adrenoceptors.

@entity1 :: ('9606', 'Species') :: ['human', 'patients', 'Human'] @entity2162 :: ('154', 'Gene') :: ['b2-AR', 'b2 adrenoceptor'] @entity6826 :: ('4254', 'Gene') :: ['SCF', 'stem cell factor'] @entity69013 :: ('MESH:C549647', 'Chemical') :: ['olodaterol'] @entity10685 :: ('2209', 'Gene') :: ['Fc  RI'] @entity23690 :: ('23705', 'Gene') :: ['CADM1', 'cell adhesion molecule 1'] @entity565 :: ('MESH:D001249', 'Disease') :: ['asthma'] @entity3045 :: ('MESH:D000420', 'Chemical') :: ['albuterol'] @entity228 :: ('CHEBI:50858', 'Chemical') :: ['corticosteroid'] @entity3960 :: ('MESH:D018235', 'Disease') :: ['airway smooth muscle', 'ASM'] @entity112 :: ('MESH:D006632', 'Chemical') :: ['histamine'] @entity6410 :: ('MESH:C012629', 'Chemical') :: ['formoterol'] @entity5023 :: ('MESH:C065382', 'Chemical') :: ['fluticasone']
@entity1 :: (9606,Species) :: ['Human']
