UNASSIGNED: The present study explores anti-MRSA ( @entity3256 resistant @entity794 ) activity of a bioactive phytochemical constituent, @entity14685 obtained from the medicinal herb, Nigella sativa Linn. Based on the initial assessment on crude extract of seeds of Nigella sativa Linn, the pure active constituent was employed in the study. Total of 99 @entity794 strains which comprised of 40 type and 59 clinical strains were selected for the study. Minimum inhibitory concentration (MIC), bactericidal activity, post-antibiotic effect (PAE) and propensity to select resistant mutants were determined using standard protocols. Results revealed that @entity14685 exhibited MIC in the range of 8 - 16  g ml(-1) and MIC90 of 16  g ml(-1) against @entity794 strains. It was bactericidal to @entity794 by demonstrating >3 log kill. It showed a longer post-antibiotic effect (PAE) of 3.2  0.2 h. Upon exposure to high density inoculum of @entity794 , it did not select resistant mutants. Transmission electron microscopy of @entity14685 treated @entity794 showed no lysis but damage to cell wall and cell membrane which corroborated well with the salt tolerance and bacteriolysis assays. In conclusion, MIC90 , bactericidal property, longer PAE, absence of resistant mutant selection and damages in cell membrane and cell wall imply a promising anti-MRSA activity of @entity14685 . This article is protected by copyright. All rights reserved.

In vitro profiling of anti-MRSA activity of XXXX against selected type and clinical strains.

@entity3256 :: ('MESH:D008712', 'Chemical') :: ['methicillin'] @entity794 :: ('1280', 'Species') :: ['Staphylococcus aureus', 'MRSA'] @entity14685 :: ('MESH:C003466', 'Chemical') :: ['thymoquinone']
@entity14685 :: (MESH:C003466,Chemical) :: ['thymoquinone']
