Antimicrobial resistance in @entity3064 is a growing public health concern and an important pathogen in @entity580 . We investigated the genes involved in resistance to @entity2888 and cephalosporins in clinical A. baumannii isolates from a tertiary medical centre in Malaysia. A. baumannii was isolated from 167 clinical specimens and identified by sequencing of the 16S rRNA and rpoB genes. The MIC for @entity2892 , meropenem, @entity5505 and @entity6944 were determined by the E-test method. The presence of carbapenemase and cephalosporinase genes was investigated by PCR. The isolates were predominantly nonsusceptible to carbapenems and @entity2886 (>70%) with high MIC values. ISAba1 was detected in all @entity2893 -nonsusceptible A. baumannii harbouring the blaOXA-23-like gene. The presence of blaOXA-51-like and ISAba1 upstream of blaOXA-51 was not associated with nonsusceptibility to carbapenems. A. baumannii isolates harbouring ISAba1-blaADC (85.8%) were significantly associated with nonsusceptibility to cephalosporins (P<0.0001). However, ISAba1-blaADC was not detected in a minority (<10%) of the isolates which were nonsusceptible to cephalosporins. The acquired OXA-23 enzymes were responsible for nonsusceptibility to carbapenems in our clinical A. baumannii isolates and warrant continuous surveillance to prevent further dissemination of this antibiotic resistance gene. The presence of ISAba1 upstream of the blaADC was a determinant for @entity5898 resistance. However, the absence of this ISAba1-blaADC in some of the isolates may suggest other resistance mechanisms and need further investigation.

Molecular characterization of carbapenemase and cephalosporinase genes among clinical isolates of XXXX in a tertiary medical centre in Malaysia.

@entity2892 :: ('MESH:D015378', 'Chemical') :: ['imipenem'] @entity6944 :: ('MESH:C043266', 'Chemical') :: ['cefepime'] @entity5898 :: ('CHEBI:23066', 'Chemical') :: ['cephalosporin'] @entity2886 :: ('MESH:D002511', 'Chemical') :: ['cephalosporins'] @entity2888 :: ('MESH:D015780', 'Chemical') :: ['carbapenems'] @entity2893 :: ('CHEBI:46765', 'Chemical') :: ['carbapenem'] @entity580 :: ('MESH:D003428', 'Disease') :: ['nosocomial infections'] @entity3064 :: ('470', 'Species') :: ['Acinetobacter baumannii'] @entity5505 :: ('MESH:D002442', 'Chemical') :: ['ceftazidime']
@entity3064 :: (470,Species) :: ['Acinetobacter baumannii']
