1. A @entity1 @entity11 cell line, NG97, was established from tissue obtained from a @entity1 diagnosed with a @entity816 . 2. The NG97 cell line has been subcultured for more than 100 passages in standard culture media without feeder layer or collagen coatings. 3. NG97 cells grow in vitro as two subpopulations with distinct morphological appearance: stellate cells with pleomorphic nuclei, and small round cells with few processes. The cells have a doubling time of about 72 h and a plating efficiency of 1%. The injection of NG97 cells into congenitally athymic @entity19 induced the formation of solid @entity5 masses that could be retransplanted every 4 weeks. The cells obtained from @entity5 mass when cultivated in vitro had a morphology comparable to those of the initial culture. 4. This cell line may prove useful for cellular and molecular studies as well as in studies of @entity11 treatment.

Establishment and partial characterization of a continuous @entity1 XXXX cell line: NG97.

@entity1 :: ('9606', 'Species') :: ['human', 'patient'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity11 :: ('MESH:D005910', 'Disease') :: ['gliomas', 'glioma'] @entity816 :: ('MESH:D001254', 'Disease') :: ['grade III astrocytoma']
@entity11 :: (MESH:D005910,Disease) :: ['malignant glioma']
