Culture conditions that support the growth of multi-and single-lineage hemopoietic colonies are also able to give rise to large @entity1582 colonies from bone marrow and peripheral blood samples of some @entity1 with @entity1582 . The culture system was used to determine the frequency of hemopoietic precursors and clonogenic @entity1582 progenitors in 71 @entity1 with @entity1582 studied in various clinical phases of the disease. The frequency of normal hemopoietic precursors in @entity1 with @entity8058 and smoldering @entity1582 were indistinguishable from normal controls. @entity1582 colonies were not observed in these subgroups. In contrast, @entity1 with active disease showed significantly reduced hemopoietic colony formation, even before the initiation of therapy. A further reduction was demonstrated for @entity1 with acute phase disease. A correlation between the frequency of hemopoietic colonies and the concentration of plasma cells in the plated sample was not observed. Large @entity1582 colonies with recloning potential were identified in cultures of specimens derived from 14 of the studied @entity1 . These colonies were most frequently (ten cases) obtained from @entity1 who had entered the acute phase of the disease. These @entity1 manifested @entity2131 ( @entity3043 ) and their marrow had a limited capacity to generate normal hemopoietic colonies. Three of the @entity1 that formed @entity1582 colonies were studied in chronic phase following chemotherapy and one @entity1 was examined at diagnosis. The @entity1582 colonies were composed exclusively of cells characterized by the same M protein as the @entity1 . Some of the cells within the colonies retained their ability to self renew extensively, as demonstrated by serial recloning studies. Colonies derived from six of the @entity1 are now propagated in semisolid and liquid medium for as many as nine to 34 generations. @entity1 that form @entity1582 colonies under these culture conditions represent a high-risk group with significantly shorter survival than @entity1 not able to give rise to @entity1582 colonies. A Cox proportional hazards model was fitted to the data to determine the prognostic role of @entity1582 colony growth in culture after accounting for the influence of other well established risk factors, such as concentration of plasma cells and disease status. The analysis indicated that @entity1582 colony growth in culture serves as a strong and independent predictive indicator of poor clinical prognosis.

Colony growth and self renewal of plasma cell precursors in XXXX .

@entity1 :: ('9606', 'Species') :: ['Patients', 'patients', 'patient'] @entity8058 :: ('MESH:D008998', 'Disease') :: ['benign monoclonal gammopathy'] @entity1582 :: ('MESH:D009101', 'Disease') :: ['multiple myeloma', 'myeloma', 'Myeloma'] @entity2131 :: ('MESH:C536572', 'Disease') :: ['marrow failure'] @entity3043 :: ('MESH:D010198', 'Disease') :: ['pancytopenia']
@entity1582 :: (MESH:D009101,Disease) :: ['multiple myeloma']
