Changes in @entity26 concentration may influence various innate characteristics of stem cells. The effects of varying @entity26 concentration on @entity1 periodontal ligament stem cells (HPDLSCs) has not been explored, particularly under @entity519 -related conditions. First, HPDLSCs were cultured from the periodontium of @entity1 teeth using the outgrowth method. STRO-1 and @entity9502 expression of HPDLSCs was investigated by flow cytometry. To detect the multilineage differentiation capacities of @entity2520 were induced in cells. Next, HPDLSCs (passage 3) were exposed to normal @entity26 (21% O2) or @entity519 (2% O2) conditions for 7 days and cell proliferation was evaluated. After culture in osteogenic medium for 7 days, osteoblastic differentiation was evaluated by semi-quantitative reverse transcription-polymerase chain reaction analysis to detect 3 osteoblastic markers: @entity8087 , osteocalcin, and @entity1096 . In addition, each cell group was incubated with a @entity692 / @entity9039 carrier and transplanted subcutaneously into the back of immunocompromised @entity19 to investigate transplantation differences in vivo. HPDLSCs were isolated, cultured, and successfully identified. After exposure of HPDLSCs to @entity519 for 7 days, the proliferation rate was increased and showed higher osteogenic differentiation potential compared to control cells. After 12 weeks of transplantation, @entity519 -treated HPDLSCs differentiated into osteoblast-like cells that formed bone-like structures. These results suggest that @entity26 concentrations affect various aspects of HPDLSC physiology and that @entity519 enhances osteogenic differentiation both in vivo and in vitro. @entity26 concentration may be a critical parameter for HPDLSCs during expansion and differentiation.

Effects of XXXX on proliferation and osteogenic differentiation of periodontal ligament stem cells: an in vitro and in vivo study.

@entity1 :: ('9606', 'Species') :: ['human'] @entity8087 :: ('860', 'Gene') :: ['core-binding factor a 1/runt-related transcription factor 2'] @entity2520 :: ('MESH:D010510', 'Disease') :: ['HPDLSCs, osteogenic-like and adipogenic-like states'] @entity9502 :: ('4162', 'Gene') :: ['CD146'] @entity9039 :: ('MESH:C018392', 'Chemical') :: ['tricalcium phosphate'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen', 'Oxygen'] @entity692 :: ('MESH:D017886', 'Chemical') :: ['hydroxyapatite'] @entity519 :: ('MESH:D000860', 'Disease') :: ['hypoxia'] @entity1096 :: ('6696', 'Gene') :: ['osteopontin']
@entity519 :: (MESH:D000860,Disease) :: ['hypoxia']
