INTRODUCTION: @entity1547 is one of the most common @entity166 affecting the oral cavity. Among the oral bacteria, mutans streptococci have been implicated as major cariogenic bacteria as they can produce high levels of @entity1547 causing substances such as @entity880 and extracellular polysaccharides. AIM: The aim of the study was to detect the presence of @entity6586 and @entity69101 in dental plaque by using Polymerase Chain Reaction (PCR) method, quantification of these micro-organisms using Modified Sucrose- @entity3637 (SB-20M) agar medium and to correlate their presence in Caries Active (CA) and @entity1547 ( @entity1547 ) pre-school @entity1 . MATERIALS AND METHODS: Sixty-eight pre-school @entity1 , in the age group of 3-5 years were divided equally into 34 CA and 34 @entity1547 @entity1 . @entity2413 samples were obtained for detection of these microorganisms by PCR method and quantification was done using SB-20M culture medium. The data was analyzed using statistical software SPSS version 16. For statistical analysis, the frequencies and means of Colony Forming Units (CFU) were used with CI = 95%. For bivariate analysis, @entity16043 exact test was used at 5% level of significance. The comparison of mean of number of CFU of @entity6586 and @entity69101 was made by Mann Whitney U test and Spearman's Rho test at 1% level of significance was used for correlation between dmft and CFU in CA group. RESULTS: The results showed that @entity69101 was significantly higher in CA group as compared to @entity1547 group whereas @entity6586 showed no significant difference. On quantification of these micro-organisms, @entity69101 was present in significantly higher numbers in CA group as compared to @entity1547 group. On correlating the CFU/ml of the micro-organisms with the dmft index, both the micro-organisms showed a positive correlation. CONCLUSION: We conclude that @entity6586 and @entity69101 were detected in higher numbers in CA @entity1 as compared to @entity1547 @entity1 . PCR is a sensitive, specific, rapid and an effective method for the detection of oral microorganisms.

Distribution of @entity6586 and @entity69101 in Dental Plaque of Indian Pre-School XXXX Using PCR and SB-20M Agar Medium.

@entity6586 :: ('1309', 'Species') :: ['Streptococcus mutans', 'S. mutans'] @entity1 :: ('9606', 'Species') :: ['children'] @entity2413 :: ('MESH:D003773', 'Disease') :: ['Dental plaque'] @entity3637 :: ('MESH:D001414', 'Chemical') :: ['Bacitracin'] @entity1547 :: ('MESH:D003731', 'Disease') :: ['Caries Free', 'Dental caries', 'CF', 'dental caries'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactic acid'] @entity69101 :: ('1310', 'Species') :: ['Streptococcus sobrinus', 'S. sobrinus'] @entity16043 :: ('76720', 'Species') :: ['Fisher'] @entity166 :: ('MESH:D003141', 'Disease') :: ['infectious diseases']
@entity1 :: (9606,Species) :: ['Children']
