AIM: To study the effect of @entity41629 (NC), a newly discovered heptadecapeptide, and @entity4596 , a @entity36226 agonist, on excitatory non-adrenergic non-cholinergic (eNANC) @entity15496 responses in @entity840 isolated bronchus. METHODS: An eNANC response was induced by electric field stimulation (EFS) in the preparation via activation of the sensory nerve terminals. The effect of NC and @entity4596 was analyzed on the response. RESULTS: Nociceptin 0.001 - 0.1 micromol/L inhibited the eNANC @entity15496 which was induced by EFS but not by @entity2951 in @entity840 bronchus. The @entity15496 inhibited by NC 0.01 micromol/L was (43 +/- 31) % compared with the control. After pretreatment with @entity1510 0.1 micromol/L, the @entity15496 was inhibited by (46 +/- 28) %, without marked change compared with the above figure. IC50 (95 % of confidence limits) was 6.12 (3.8 - 9.9) nmol/L. @entity4596 also inhibited the EFS-evoked eNANC @entity15496 and the effect was abolished after pretreatment with @entity1510 . IC50 (95 % of confidence limits) was 1.08 (0.5 - 2.2) micromol/L. @entity2951 0.01 - 1 micromol/L caused a cumulative @entity15496 response in the preparation. Moreover, the effect of @entity2951 was not affected by pretreatment with NC 0.01 micromol/L or @entity4596 0.1 micromol/L. The @entity15496 induced by exogenous @entity14945 , were also unaffected by treatment with NC 0.01 micromol/L or @entity4596 0.1 micromol/L in isolated bronchus. CONCLUSION: Nociceptin inhibits EFS-induced eNANC @entity15496 , which is not reversed by @entity1510 , while @entity4596 inhibits EFS-induced eNANC response via activation of opioid receptor in @entity840 airways.

Inhibition by nociceptin on excitatory non-adrenergic non-cholinergic response in XXXX airways.

@entity41629 :: ('MESH:C096012', 'Chemical') :: ['nociceptin'] @entity2951 :: ('MESH:D002211', 'Chemical') :: ['Capsaicin', 'capsaicin'] @entity840 :: ('10141', 'Species') :: ['guinea pig'] @entity4596 :: ('MESH:D019900', 'Chemical') :: ['U-50488H'] @entity1510 :: ('MESH:D009270', 'Chemical') :: ['naloxone'] @entity14945 :: ('MESH:D015288', 'Chemical') :: ['neurokinin A'] @entity36226 :: ('100135587(Tax:10141)', 'Gene') :: ['kappa-opioid receptor'] @entity15496 :: ('MESH:D015877', 'Disease') :: ['constriction']
@entity840 :: (10141,Species) :: ['guinea pig']
