PURPOSE: To examine the applicability of culture supernatant of @entity1 amniotic cells on @entity540 ( @entity540 )-induced @entity7499 . METHODS: @entity1 amniotic epithelial and mesenchymal cells (AC) were obtained from @entity1 amniotic membranes by digesting with collagenase and maintained in serum-containing medium. The AC preparations predominantly contained cytokeratin-positive cells (91.2 +/- 3.1%, n = 4). The culture supernatant was prepared by cultivating AC in serum-free medium for 24 hours. Neovascularization was obtained by a micropocket assay with @entity55206 pellets containing @entity540 . Migration assay was carried out by a double-chamber method using @entity1 umbilical vein endothelial cells (HUVEC). Cell growth assay was done by @entity98 assay using HUVEC. RESULTS: @entity540 -induced @entity7499 was significantly reduced by administration of AC culture supernatant. When either control or AC culture supernatant was administered three times per day for 10 days, the area with @entity2295 was 13.2 +/- 3.2 mm2 and 4.0 +/- 1.4 mm2 for the control and AC culture supernatant-treated eyes, respectively (n = 7, p = 0.021). AC culture supernatant strongly inhibited @entity540 -induced @entity4222 and cell growth of HUVEC. CONCLUSIONS: Amniotic cell culture supernatant contains potent @entity2295 . This effect is explained in part by inhibition of @entity4222 and cell growth of vascular endothelial cells. AC culture supernatant may be applicable for treatment of @entity2687 with @entity2295 .

Suppression of @entity7499 by culture supernatant of XXXX amniotic cells.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity7499 :: ('MESH:D016510', 'Disease') :: ['corneal neovascularization'] @entity4222 :: ('MESH:D014085', 'Disease') :: ['migration'] @entity2295 :: ('MESH:D015861', 'Disease') :: ['neovascularization', 'inhibitors of neovascularization'] @entity540 :: ('2247', 'Gene') :: ['Basic fibroblast growth factor', 'bFGF', 'basic fibroblast growth factor'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity2687 :: ('MESH:D003316', 'Disease') :: ['corneal diseases'] @entity55206 :: ('MESH:C000257', 'Chemical') :: ['Hydron']
@entity1 :: (9606,Species) :: ['human']
