PURPOSE: Mesenchymal stem/stromal cells (MSCs) facilitate the regeneration of injured tissue. Our group has previously shown that @entity1 MSCs (hMSCs) or hMSC-derived factors suppress excessive inflammatory response in the @entity267 following @entity130 in vivo. We here investigated direct effects of hMSC-derived factors on cultures of chemically injured @entity1 corneal epithelial progenitor cells (hCEP), independent of systemic anti-inflammatory effects that hMSCs have been shown to have in vivo. METHODS: We injured hCEP by incubation in 20% @entity1068 for 30 seconds, and cultured the cells in fresh medium or in medium derived from cultures of @entity1 dermal fibroblasts (hFbs), hMSCs, or @entity310 -activated hMSCs. After 24 hours, we evaluated the survival, proliferation, and apoptosis of the cells. RESULTS: The hMSC-conditioned medium enhanced survival and proliferation and inhibited apoptosis of chemically injured hCEP. In addition, the conditioned medium accelerated the wound healing of corneal epithelium in tissue cultures of @entity95 corneas following injury. The effects of the hMSC-conditioned medium were increased by preincubating hMSCs with @entity310 . The increased effectiveness of the medium from the preactivated hMSCs was in part explained by increased concentration of the multifunctional protein @entity25917 that inhibits apoptosis and promotes survival of cells. CONCLUSIONS: Together, the data account for beneficial effects of hMSCs on tissue-endogenous stem cells involving hCEP, and provide a basis for using MSCs or MSC-derived factors to treat @entity66 and other tissues.

Effects of mesenchymal stem/stromal cells on cultures of corneal epithelial progenitor cells with XXXX injury.

@entity1 :: ('9606', 'Species') :: ['human'] @entity267 :: ('MESH:D065306', 'Disease') :: ['cornea'] @entity66 :: ('MESH:D004194', 'Disease') :: ['diseases of the cornea'] @entity310 :: ('7124', 'Gene') :: ['TNF-a'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity25917 :: ('6781', 'Gene') :: ['stanniocalcin-1'] @entity1068 :: ('MESH:D000431', 'Chemical') :: ['ethanol'] @entity130 :: ('MESH:D014947', 'Disease') :: ['chemical injury']
@entity1068 :: (MESH:D000431,Chemical) :: ['ethanol']
