BACKGROUND: The purpose of this study is to determine whether microRNA for pluripotent stem cells are also expressed in @entity0 and are associated with @entity3 and outcome. METHODS: We studied global microRNA profiles during differentiation of @entity1 embryonic stem cells (n =26) and in @entity0 @entity1 (n = 33) and @entity1 cell lines (n = 35). Using in situ hybridization, we then investigated MIR302 expression in 318 untreated @entity0 @entity1 (test cohort, n = 22 and validation cohort, n = 296). In parallel, using next-generation sequencing data from @entity0 @entity1 (n = 684), we assessed microRNA association with stem cell markers. All statistical tests were two-sided. RESULTS: In healthy tissues, the MIR302 (high)/ @entity20157 (low) asymmetry was exclusive for pluripotent stem cells. MIR302 was expressed in a small population of @entity5 cells @entity513 , but not in normal breast (P < .001). Furthermore, MIR302 was expressed in the @entity5 cells together with stem cell markers, such as @entity1759 and @entity3752 . Conversely, @entity20157 expression in 684 @entity0 negatively correlated with @entity1759 (Spearman correlation, Rho = -0.08, P = .04) and @entity3752 (Rho = -0.11, P = .004), but positively correlated with differentiation marker @entity11707 (Rho = 0.15, P < .001). @entity5 with lymph node @entity3 had @entity5 cells showing scattered expression of MIR302 and widespread repression of @entity20157 . Finally, overall survival was statistically significantly shorter in @entity1 with MIR302-positive @entity5 cells (P = .03). CONCLUSIONS: In healthy tissues the MIR302(high)/ @entity20157 (low) asymmetry was characteristic of embryonic and induced pluripotency. In @entity513 , the MIR302/ @entity20157 asymmetry was associated with stem cell markers, @entity3 , and shorter survival.

Pluripotent stem cell miRNAs and @entity3 in invasive XXXX .

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer', 'breast tumors'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'Primary tumors'] @entity3752 :: ('648', 'Gene') :: ['BMI1'] @entity11707 :: ('100133941', 'Gene') :: ['CD24'] @entity1759 :: ('960', 'Gene') :: ['CD44'] @entity513 :: ('MESH:D044584', 'Disease') :: ['within invasive ductal carcinoma', 'invasive ductal carcinoma'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity20157 :: ('406986', 'Gene') :: ['MIR203']
@entity0 :: (MESH:D001943,Disease) :: ['breast cancer']
