1. Two mutants of @entity2624 were isolated which, although able to grow on @entity413 , are unable to grow with @entity4588 or @entity6853 as the sole source of @entity505 . 2. Genetic mapping of these mutants showed that they both contain a mutation in a gene (designated uncA) mapping at about minute 73.5 on the @entity526 chromosome. 3. The uncA(-) alleles were transferred by bacteriophage-mediated transduction into another strain of @entity526 and the transductants compared with the parent strain to determine the nature of the biochemical lesion in the mutants. 4. The mutants gave low aerobic growth yields when grown on limiting concentrations of @entity413 , but oxidase activities in membranes from both the mutants and the normal strain were similar. 5. Measurement of P/O ratios with @entity6853 as substrate indicated that a mutation in the uncA gene causes uncoupling of phosphorylation associated with electron transport. 6. Determination of the @entity1025 , @entity141 -stimulated @entity859 triphosphatase activities in the mutant and normal strains indicated that the uncA gene is probably the structural gene for @entity1025 , @entity141 -stimulated @entity859 triphosphatase. 7. @entity1025 , @entity141 -stimulated @entity859 triphosphatase therefore appears to be essential for oxidative phosphorylation in @entity526 .

Oxidative phosphorylation in @entity2624 . Mutations affecting magnesium ion- or calcium ion-stimulated XXXX triphosphatase.

@entity859 :: ('MESH:D000241', 'Chemical') :: ['adenosine'] @entity1025 :: ('CHEBI:18420', 'Chemical') :: ['Mg(2+)'] @entity4588 :: ('MESH:D019802', 'Chemical') :: ['succinate'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity6853 :: ('CHEBI:16004', 'Chemical') :: ['d-lactate'] @entity2624 :: ('83333', 'Species') :: ['Escherichia coli K 12'] @entity505 :: ('MESH:D002244', 'Chemical') :: ['carbon'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity526 :: ('562', 'Species') :: ['E. coli']
@entity859 :: (MESH:D000241,Chemical) :: ['adenosine']
