BACKGROUND _ AIMS: @entity68 may catalyze the production of reactive @entity26 species (ROS) during postischemic reoxygenation. Ferritin, a cellular @entity68 storage protein, can either represent a source of @entity68 or perform a cytoprotective action against ROS. The aim of this study was to address the role of ferritin in postischemic reperfusion. METHODS: Transcriptional and posttranscriptional mechanisms controlling ferritin gene expression were studied in reperfused @entity35 livers. RESULTS: Proteolysis reduced ferritin levels 2 hours after reperfusion, but a concomitant increase of synthesis, accompanied by enhanced transcription and accumulation of H and L ferritin subunit messenger RNAs (mRNAs), almost re-established normal ferritin content at 4 hours. Pretreatment with @entity19646 ( @entity19646 ) did not prevent the rise of ferritin mRNAs. RNA bandshift assays showed that the activity of the @entity68 regulatory proteins (IRPs), which control ferritin mRNA translation, declined early after reperfusion and recovered progressively thereafter. Pretreatment with either the antioxidant @entity898 or @entity19646 was sufficient to prevent almost completely down-regulation of @entity4124 activity. CONCLUSIONS: Postischemic reperfusion causes degradation of ferritin, possibly increasing @entity68 levels. However, induction of ferritin gene transcription, possibly mediated by ferritin-derived @entity68 and ROS-mediated inactivation of @entity4124 , which allows translation of ferritin mRNAs, counteracts this effect and concurs to reestablish the amount of ferritin, which may thus act to limit reperfusion damage.

Induction of ferritin synthesis in ischemic-reperfused XXXX liver: analysis of the molecular mechanisms.

@entity68 :: ('MESH:D007501', 'Chemical') :: ['iron', 'Iron'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity898 :: ('MESH:D000111', 'Chemical') :: ['N-acetyl cysteine'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity4124 :: ('25402(Tax:10116)', 'Gene') :: ['IRP'] @entity19646 :: ('60582(Tax:10116)', 'Gene') :: ['IL-1RA', 'interleukin 1 receptor antagonist']
@entity35 :: (10116,Species) :: ['rat']
