OBJECTIVE: To develop a rapid method to quantify @entity3999 in muscle biopsy specimens from @entity1 with @entity3124 ( @entity3124 ). DESIGN: Percutaneous muscle biopsy specimens at different stages of @entity3124 were examined by light microscopy and @entity1579 ( @entity1579 ) and by horizontal pore gradient @entity214 electrophoresis ( @entity214 -PAGE). The @entity3999 /actin ratio was calculated densitometrically. Neurophysiological examinations were performed at various times during the course of @entity3124 . SETTING: All @entity1 were treated in intensive care units at Karolinska Hospital. @entity1 AND @entity1 : We studied 11 @entity1 with @entity3124 , 5 @entity1 with @entity16 , and 42 control individuals. MEASUREMENTS AND RESULTS: The histopathological changes included in all cases muscle fiber @entity194 , degeneration, regeneration, nuclear changes, and reduction in myofibrillar ATPase activity in both type I and II fibers. In severely affected muscles fiber type differentiation was lost. On @entity1579 preferential loss of thick filaments was the dominant finding. In some @entity1 changes were present only in parts of the specimen. The neurophysiological examinations indicated @entity121 in five @entity1 and combined @entity121 and @entity16 in five and suggested @entity16 in one. The @entity214 -pore PAGE used showed a technical variation of 4-5%. Quantitative results were obtained within 1 day and night. The mean value of the @entity3999 /actin ratio in controls was 1.37+/-0.21 and in @entity3124 @entity1 0.37+/-0.17, without overlapping with the control values. CONCLUSIONS: Considering the diagnostic difficulty using morphological and neurophysiological methods, especially in early stages of @entity3124 , we suggest including @entity214 -pore PAGE to determine the @entity3999 /actin ratio for rapid diagnosis of @entity3124 .

Electrophoretic determination of the XXXX /actin ratio in the diagnosis of @entity3124 .

@entity1 :: ('9606', 'Species') :: ['patients', 'PATIENTS', 'PARTICIPANTS'] @entity3124 :: ('MESH:D016638', 'Disease') :: ['CIM', 'critical illness myopathy'] @entity16 :: ('MESH:D009422', 'Disease') :: ['axonal neuropathies', 'neuropathy'] @entity1579 :: ('MESH:D017096', 'Disease') :: ['transmission electron microscopy', 'TEM'] @entity3999 :: ('79784', 'Gene') :: ['myosin'] @entity194 :: ('MESH:D001284', 'Disease') :: ['atrophy'] @entity121 :: ('MESH:D009135', 'Disease') :: ['myopathy'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS']
@entity3999 :: (79784,Gene) :: ['myosin']
