BACKGROUND: Little is known presently about the effects of nonsteroidal anti-inflammatory drugs (NSAIDs) on the longitudinal bone growth occurring in the growth plate. We have examined the effects of administration of different types of NSAIDs on the longitudinal growth of the growth plate using a fetal @entity35 metatarsal bone culture model. METHODS: Cultured fetal @entity35 metatarsal bones were used to study the effect of nonselective ( @entity3352 ) and selective @entity4645 ( @entity4645 , @entity12698 ) NSAIDs on longitudinal bone growth. The effect of NSAIDs on proliferation and apoptosis of growth plate chondrocytes were analyzed by @entity1603 incorporation and a TUNEL assay. @entity1421 ( @entity1421 ) production was measured by an ELISA kit. We also examined the effect of exogenous @entity1421 on the growth plate. RESULTS: NSAIDs caused a dose-dependent @entity851 of cultured metatarsal bones. Both nonselective and @entity4645 selective NSAIDs inhibit longitudinal bone growth. We found that NSAIDs suppressed the proliferation of chondrocytes and production of @entity1421 , and increased the apoptosis of chondrocytes. Supplemental @entity1421 could not reverse the effects of NSAIDs on the growth plate. CONCLUSIONS: Our data show that NSAIDs induce a dose-dependent @entity851 of cultured @entity35 metatarsal bones. A detailed analysis revealed decreased proliferation and increased apoptosis of chondrocytes in the growth plate, indicating that @entity4645 is responsible for @entity1421 production in growth plate chondrocytes. CLINICAL RELEVANCE: The data from the present study suggest that deleterious effects on the growth plate by chronic NSAIDs use should be considered for @entity1 who have chronic inflammatory diseases, such as @entity309 .

Nonsteroidal Anti-Inflammatory Drugs Cause Inhibition of the Growth Plate in Cultured XXXX Metatarsal Bones.

@entity1 :: ('9606', 'Species') :: ['children'] @entity1603 :: ('MESH:D001973', 'Chemical') :: ['BrdU'] @entity12698 :: ('MESH:C105934', 'Chemical') :: ['celecoxib'] @entity851 :: ('MESH:D006130', 'Disease') :: ['growth retardation'] @entity1421 :: ('MESH:D015232', 'Chemical') :: ['PGE2', 'Prostaglandin E2'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity3352 :: ('MESH:D007213', 'Chemical') :: ['indomethacin'] @entity309 :: ('MESH:D001172', 'Disease') :: ['juvenile rheumatoid arthritis'] @entity4645 :: ('29527(Tax:10116)', 'Gene') :: ['COX-2', 'cyclooxygenase-2']
@entity35 :: (10116,Species) :: ['Rat']
