BACKGROUND: High expression of the long non-coding RNA nuclear-enriched abundant transcript 1 ( @entity23844 ) in whole blood has been reported in @entity14 @entity1 ; however, its' clinical significance and origin are unclear. We evaluated the diagnostic and prognostic value, and origin of whole blood @entity23844 in @entity14 . METHODS: Expression of @entity23844 variants, @entity23844 _v1 and @entity23844 _v2 were determined using real-time quantitative PCR. The diagnostic value of whole blood @entity23844 expression was evaluated in test (n = 60) and validation (n = 200) cohorts of @entity14 @entity1 and normal controls (NCs). To identify the origin of @entity23844 , its expression was analyzed in blood, matched @entity5 tissues, para- @entity5 tissues, metastatic tissues, and also immune cells from @entity1 or NCs. Function of @entity23844 in colorectal cell lines was also assessed. The correlation of @entity23844 expression with clinical outcomes was assessed in 191 @entity1 . RESULTS: Whole blood @entity23844 expression was significantly higher in @entity14 @entity1 than in NCs. @entity23844 _v1 and @entity23844 _v2 expression were highly accurate in distinguishing @entity14 @entity1 from NCs (area under the curve: 0.787 and 0.871, respectively). Knockdown of @entity23844 _v1 in vitro could inhibit cell invasion and proliferation, while knockdown of @entity23844 _v2 promoted cell growth. However, whole blood expression was not correlated with matched tissues. An elevated expression was seen in neutrophils from @entity14 @entity1 . Furthermore, high expression of @entity23844 _v1 was correlated with worse overall survival. In contrast, high expression of @entity23844 _v2 alone was correlated with better overall survival. CONCLUSION: Whole blood @entity23844 expression is a novel diagnostic and prognostic biomarker of overall survival in @entity14 . Elevated @entity23844 may derive from neutrophils.

Nuclear-enriched abundant transcript 1 as a diagnostic and prognostic biomarker in XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity23844 :: ('283131', 'Gene') :: ['NEAT1'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'primary tumor'] @entity14 :: ('MESH:D015179', 'Disease') :: ['CRC', 'colorectal cancer']
@entity14 :: (MESH:D015179,Disease) :: ['colorectal cancer']
