UNASSIGNED: @entity5 is associated with @entity281 by certain types of @entity1082 ). The gene variants differ in immune responses and oncogenic potential. The @entity32454 proteins encoded by high-risk HPV play a key role in cellular transformation. HPV-33 and HPV-58 types are highly prevalent among Chinese @entity1 . To study the gene intratypic variations, polymorphisms and positive selections of HPV-33 and HPV-58 E6/E7 in southwest China, HPV-33 (E6, E7: n = 216) and HPV-58 (E6, E7: n = 405) @entity32454 genes were sequenced and compared to others submitted to GenBank. Phylogenetic trees were constructed by Maximum-likelihood and the Kimura 2-parameters methods by MEGA 6 (Molecular Evolutionary Genetics Analysis version 6.0). The diversity of secondary structure was analyzed by PSIPred software. The selection pressures acting on the E6/E7 genes were estimated by PAML 4.8 (Phylogenetic Analyses by Maximun Likelihood version4.8) software. The positive sites of HPV-33 and HPV-58 E6/E7 were contrasted by ClustalX 2.1. Among 216 HPV-33 E6 sequences, 8 single @entity4334 mutations were observed with 6/8 non-synonymous and 2/8 synonymous mutations. The 216 HPV-33 E7 sequences showed 3 single @entity4334 mutations that were non-synonymous. The 405 HPV-58 E6 sequences revealed 8 single @entity4334 mutations with 4/8 non-synonymous and 4/8 synonymous mutations. Among 405 HPV-58 E7 sequences, 13 single @entity4334 mutations were observed with 10/13 non-synonymous mutations and 3/13 synonymous mutations. The selective pressure analysis showed that all HPV-33 and 4/6 HPV-58 E6/E7 major non-synonymous mutations were sites of positive selection. All variations were observed in sites belonging to major histocompatibility complex and/or B-cell predicted epitopes. @entity69288 and R145 (I/N) were observed in both HPV-33 and HPV-58 E6.

@entity32454 Gene Polymorphisms in XXXX Types-58 and 33 Identified in Southwest China.

@entity1 :: ('9606', 'Species') :: ['women'] @entity5 :: ('MESH:D009369', 'Disease') :: ['Cancer of the cervix'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity32454 :: ('25479186', 'Gene') :: ['E6 and E7'] @entity1082 :: ('10566', 'Species') :: ['human papillomavirus (HPV'] @entity69288 :: ('p|SUB|K|93|N', 'ProteinMutation') :: ['K93N'] @entity4334 :: ('D009711', 'Chemical') :: ['nucleotide']
@entity1082 :: (10566,Species) :: ['Human Papillomavirus']
