OBJECTIVE: To investigate the correlation between the expressions of apoptosis-promoting gene @entity2310 , apotosis-inhibiting gene @entity1976 , and Fas/ @entity23975 in gastric mucosa in the course of stress ulcer. METHODS: Fifty-six @entity35 were exposed to @entity2700 ( @entity2700 ) and then randomly divided into 7 groups of 8 @entity35 : @entity2700 2 h, RS 3.5 h, and 2 h, 5 h, 8 h, 12 h, and 24 h after termination of @entity2700 . Another 8 @entity35 were used as controls. The stomachs of @entity35 were taken out at the above mentioned time points to observe histologically. Terminal deoxynucleatidyl transferase mediated @entity1977 nick and labeling (TUNEL) technique was used to examine the apoptotic cells in the gastric mucosa. The expression of @entity1976 / @entity2310 protein was examined by immunohistochemical assay. In situ hybridization was used to detect the mRNA expression of @entity1976 , Fas, and @entity23975 . RESULTS: (1) Apototic cells were seen sporadically in the gastric mucosa of the control @entity35 . The number of apoptotic cells began to increase since 2 hours after the beginning of WPS and reached its peak 5 hours after the termination of @entity2700 . Since 8 - 12 hours after the termination of @entity2700 the number of apoptosis began to decrease, however, still remained at a level significantly higher than the normal level (P < 0.05). (2) Fas mRNA and @entity23975 mRNA were not expressed in the controls. Fas mRNA was detected 5 - 8 hours after the termination of @entity2700 at the base of mucosa at a low level and disappeared 8 hours after the termination of @entity2700 . @entity23975 mRNA was detected since @entity2700 3.5h to 8 hours after the termination of @entity2700 . (3) In normal gastric mucosa, Bcl-1 mRNA and protein were expressed in gastric gland zone at a middle level and @entity2310 protein was expressed in the epithelial cells. In @entity2700 @entity35 @entity2310 protein expression began to be detected in the epithelial cells lining the gastric pits since @entity2700 2 h, then increased, reached the peak 2 - 5 hours after the termination of @entity2700 , and then gradually decreased and remained a little higher than normal level 24 hours later (P < 0.05). @entity1976 expression began to decrease since the termination of @entity2700 and then increased 8 - 12 hours after the termination of @entity2700 and reached the normal level 24 hours later (P > 0.05). Correlation analysis showed that the expression of @entity2310 protein was correlated with the quantity of @entity356 . CONCLUSION: The alterations of Bcl and @entity2310 may be responsible for the development of @entity75 and recovery. The expression of Fas/ @entity23975 has no effect on the @entity1819 related to @entity2700 . Overexpression of @entity1976 may inhibit the Fas/ @entity23975 induced apoptosis.

[Expression and function of apoptosis-related genes XXXX / @entity2310 and Fas/ @entity23975 in the course of stress ulcer].

@entity2700 :: ('MESH:D003681', 'Disease') :: ['WRS', 'water immersion-restraint stress'] @entity1976 :: ('24224(Tax:10116)', 'Gene') :: ['Bcl-2'] @entity1819 :: ('MESH:D013272', 'Disease') :: ['gastric mucosa apoptosis'] @entity2310 :: ('24887(Tax:10116)', 'Gene') :: ['Bax'] @entity75 :: ('MESH:D001927', 'Disease') :: ['mucosa lesion'] @entity23975 :: ('25385(Tax:10116)', 'Gene') :: ['FasL'] @entity1977 :: ('CHEBI:17625', 'Chemical') :: ['dUTP'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity356 :: ('MESH:D013274', 'Disease') :: ['apotosis in gastric mucosa']
@entity1976 :: (24224(Tax:10116),Gene) :: ['Bcl-2']
