UNASSIGNED: Although the mitochondrial metabolism responses to warm acclimation have been widely studied in fish, the time course of this process is less understood. Here, we characterise changes of @entity5660 (Oncorhyncus mykiss) cardiac mitochondrial metabolism during acute warming from 10 to 16  C, and during the subsequent warm acclimation for 39 days (D). We repeatedly measured mitochondrial @entity3612 consumption in cardiac permeabilized fibers and functional integrity of mitochondria (i.e. mitochondrial coupling and cytochrome c effect) at two assay temperatures (10 and 16  C), as well as @entity3736 synthase (CS) and @entity880 dehydrogenase (LDH) activities at room temperature. LDH and CS activities significantly increased between D0 (10  C acclimated fish) and D1 (acute warming to 16  C), while mitochondrial @entity3612 consumption measured at respective in vivo temperatures did not change. Enzymatic activities and mitochondrial @entity3612 consumption rates significantly decreased by D2, and remained stable during warm acclimation (D2-39). The decrease in rates of @entity3612 between D0 and D1 coincided with an increased cytochrome c effect and a decreased mitochondrial coupling, suggesting a @entity2383 of mitochondria during acute warming. We suggest that after two days of warm acclimation, a new homeostasis is reached, which may involve @entity409 . Interestingly, from D2 onward, there was a lack of differences in mitochondrial @entity3612 consumption rates between the assay temperatures, suggesting that warm acclimation reduces the acute thermal sensitivity of mitochondria. This study provides significant knowledge on the thermal sensitivity of cardiac mitochondria that is essential to delineate the contribution of cellular processes to warm acclimation.

Dynamic changes in cardiac mitochondrial metabolism during warm acclimation in XXXX .

@entity5660 :: ('8022', 'Species') :: ['rainbow trout'] @entity409 :: ('MESH:D008107', 'Disease') :: ['removal of dysfunctional mitochondria'] @entity3736 :: ('C102006', 'Chemical') :: ['citrate'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity3612 :: ('D013481', 'Chemical') :: ['O2'] @entity2383 :: ('MESH:D028361', 'Disease') :: ['structural/functional impairment']
@entity5660 :: (8022,Species) :: ['rainbow trout']
