BACKGROUND: Periodontal ligament fibroblasts (PDLFs), which can be differentiated into osteoblasts, are crucial cells for the regeneration of the periodontal tissue. Although @entity1024 ( @entity143 ) receptors were reported to be involved in bone formation by affecting osteoblasts, the existence and function of @entity143 receptors in PDLFs have not been confirmed. The purpose of this study was to examine the expression of @entity143 receptors and their role in @entity1 PDLFs. METHODS: @entity1 PDLFs were cultured and evaluated to identify the subunits of @entity143 receptors (NR) by reverse transcription-polymerase chain reaction, Western blot analysis, and immunocytochemistry. Then, the cells were assigned to four different groups: a control media group, a control media with @entity143 receptor antagonist group, a differentiation media group, and a differentiation media with @entity143 receptor antagonist group. Cell proliferation assay, @entity623 ( @entity623 ) activity analysis, and mineralization assay were performed to determine whether @entity143 receptors affected the function of PDLFs. RESULTS: @entity6806 , @entity13069 , and @entity21412 were detected in @entity1 PDLFs. There was no statistically significant difference in proliferation among the groups. However, the @entity143 receptor antagonist-treated group showed a significant reduction in @entity623 activity (P <0.05). Moreover, the @entity143 receptor antagonist-supplemented group presented no mineralization. CONCLUSIONS: This study revealed the existence of @entity143 receptors in @entity1 PDLFs and specified their subunits. Moreover, @entity143 receptors had a significant influence on the differentiation and mineralization of @entity1 PDLFs but did not affect their proliferation. These results suggest that @entity143 receptors may play an important role in the differentiation and mineral tissue formation of @entity1 PDLFs.

Identification of @entity1024 receptor subunit in XXXX periodontal ligament fibroblasts: potential role in regulating differentiation.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity1024 :: ('CHEBI:31882', 'Chemical') :: ['N-methyl-D-aspartate'] @entity6806 :: ('2902', 'Gene') :: ['NR1'] @entity143 :: ('CHEBI:6121', 'Chemical') :: ['NMDA'] @entity21412 :: ('2906', 'Gene') :: ['NR2D'] @entity623 :: ('250', 'Gene') :: ['ALP', 'alkaline phosphatase'] @entity13069 :: ('2904', 'Gene') :: ['NR2B']
@entity1 :: (9606,Species) :: ['human']
