@entity141 entry under resting conditions may be important for contraction of vascular smooth muscle, but little is known about the mechanisms involved. @entity141 leakage was studied in the A7r5 smooth muscle-derived cell line by patch-clamp techniques. Two channels that could mediate @entity460 influx at resting membrane potentials were characterized. In 110 mM @entity8594 , one channel had a slope conductance of 6.0 +/- 0.6 pS and an extrapolated reversal potential of +41 +/- 13 mV (mean +/- SD, n = 8). The current rectified strongly, with no detectable outward current, even at +90 mV. Channel gating was voltage independent. A second type of channel had a linear current-voltage relationship, a slope conductance of 17.0 +/- 3.2 pS, and a reversal potential of +7 +/- 4 mV (n = 9). The open probability increased e-fold per 44 +/- 10 mV depolarization (n = 5). Both channels were also observed in 110 mM @entity141 . Noise analysis of whole-cell currents indicates that approximately 100 6-pS channels and 30 17-pS channels are open per cell. These 6-pS and 17-pS channels may contribute to resting @entity460 entry in vascular smooth muscle cells.

Multiple channels mediate XXXX leakage in the A7r5 smooth muscle-derived cell line.

@entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+'] @entity8594 :: ('MESH:C080430', 'Chemical') :: ['Ba2+'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium']
@entity460 :: (MESH:D002118,Chemical) :: ['calcium']
