The membrane-bound hydrogenases of @entity19062 , @entity18394 , Alcaligenes latus, and @entity1486 were purified extensively and compared. @entity3208 - @entity2975 gel electrophoresis of each hydrogenase revealed two prominent protein bands, one near 60 kilodaltons and the other near 30 kilodaltons. The migration distances during nondenaturing @entity2975 gel electrophoresis were similar for all except A. vinelandii hydrogenase, which migrated further than the other three. The @entity535 composition of each hydrogenase was determined, revealing substantial similarity among these enzymes. This was confirmed by calculation of S delta Q values, which ranged from 8.0 to 26.7 S delta Q units. S delta Q is defined as sigma j(Xi,j-Xk,j)2, where i and k identify the proteins compared and Xj is the content (residues per 100) of a given @entity535 of type j. The hydrogenases of this study were also compared with an enzyme-linked immunosorbent assay. Antibody raised against @entity19063 hydrogenase cross-reacted with all four hydrogenases, but to various degrees and in the order @entity19063 greater than A. latus greater than A. eutrophus greater than A. vinelandii. Antibody raised against A. eutrophus hydrogenase also cross-reacted with all four hydrogenases, following the pattern of cross-reaction A. eutrophus greater than A. latus = @entity19063 greater than A. vinelandii. Antibody raised against @entity19063 hydrogenase inhibited @entity19063 hydrogenase activity to a greater extent than the A. eutrophus and A. latus activities; no inhibition of A. vinelandii hydrogenase activity was detected. The results of these experiments indicated remarkable homology of the hydrogenases from these four microorganisms.

Molecular and immunological comparison of membrane-bound, H2-oxidizing hydrogenases of XXXX , @entity18394 , Alcaligenes latus, and @entity1486 .

@entity19063 :: ('373177', 'Species') :: ['B. japonicum'] @entity3208 :: ('MESH:D012967', 'Chemical') :: ['Sodium dodecyl sulfate'] @entity18394 :: ('106590', 'Species') :: ['Alcaligenes eutrophus'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity19062 :: ('375', 'Species') :: ['Bradyrhizobium japonicum'] @entity1486 :: ('354', 'Species') :: ['Azotobacter vinelandii']
@entity19062 :: (375,Species) :: ['Bradyrhizobium japonicum']
