UNASSIGNED: It has been reported that remote @entity63 postconditioning was able to protect from a harmful @entity63 occurring in brain. In the present study, we investigated the role of @entity12220 MAPK signal pathway in the process of neuroprotection and anti- @entity66 following remote limb @entity63 postconditioning on @entity35 focal @entity2806 /reperfusion (I/R) model. Male @entity35 were divided randomly into four groups: the sham-operated group, I/R group, limb @entity63 postconditioning (LPostC) group, and LPostC+ @entity1441 ( @entity12220 MAPK inhibitor) group. Focal @entity63 was induced by transient @entity2500 . Limb @entity63 postconditioning was implemented by brief cycles of femoral @entity432 . At 24h after modeling, we analyzed the @entity51 score, assessed the cerebral tissue morphology by H-E staining, and evaluated neuronal @entity66 by TUNEL staining. The protein expression levels of p- @entity12220 or p-ATF2 (phospho-activating transcription factor 2) in the penumbra region were detected by western blotting or immunohistochemical staining. Our findings revealed that LPostC relieved @entity2806 /reperfusion injury by decreasing neurological score, improving neuronal morphological changes in the @entity63 penumbra area, and reducing neuronal @entity66 . In addition, LPostC or LPostC+ @entity1441 attenuated the increase in p- @entity12220 and p-ATF2 levels in @entity63 /reperfusion brain tissue. These results indicate that the protective effects of LPostC against @entity2806 may be related to the attenuation of neuronal @entity66 and the suppression of @entity24783 pathway.

The role of p38MAPK signal pathway in the neuroprotective mechanism of limb postconditioning against @entity35 XXXX /reperfusion injury.

@entity66 :: ('MESH:D004194', 'Disease') :: ['apoptosis'] @entity2806 :: ('MESH:D002545', 'Disease') :: ['cerebral I/R injury', 'cerebral ischemia'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemia', 'ischemic'] @entity2500 :: ('MESH:D020244', 'Disease') :: ['middle cerebral artery occlusion'] @entity1441 :: ('MESH:C093642', 'Chemical') :: ['SB203580'] @entity51 :: ('MESH:D009461', 'Disease') :: ['neurological deficit'] @entity432 :: ('MESH:D001157', 'Disease') :: ['artery occlusion'] @entity35 :: ('10116', 'Species') :: ['rat', 'Sprague-Dawley rats'] @entity12220 :: ('81649(Tax:10116)', 'Gene') :: ['p38'] @entity24783 :: ('81647(Tax:10116)', 'Gene') :: ['p38 MAPK-ATF2']
@entity2806 :: (MESH:D002545,Disease) :: ['cerebral ischemia']
