UNASSIGNED: Short-hairpin RNA of frizzled-2 (shRNA- @entity13032 ) is known to suppress the proliferation of @entity157 ( @entity157 ) cells; however, its effect on @entity157 cell motility is unknown. In this study, suppression of @entity157 cell motility by shRNA- @entity13032 was analyzed, and introduction of shRNA- @entity13032 into @entity157 cells was facilitated with ultrasound (US) irradiation generated from a diagnostic US device, which was enhanced by the contrast-enhanced US reagent Sonazoid. The @entity157 cell lines HLF and @entity9294 /PRF/5 that were transfected with shRNA- @entity13032 were plated to form monolayers, following which the cell monolayers were scratched with a sterile razor. After 48 h, the cells were stained with @entity1710 and @entity1711 , and the distance between the growing edge of the cell layer and the scratch lines was measured. Total RNA from the cells was isolated and subjected to real-time quantitative PCR to quantify @entity1495 expression at 48  h after transfection of shRNA- @entity13032 . Starch- @entity6893 method was applied to analyze the generation of @entity5568 following US irradiation with the addition of Sonazoid in the liquid, and cell proliferation was analyzed 72  h later. The distances between the growing edge of the cell layer and the scratch lines and @entity1495 expression levels were significantly decreased with transfection of shRNA- @entity13032 (P<0.05). In the starch- @entity6893 method, absorbance significantly decreased with the addition of Sonazoid (P<0.05), which suggested that US irradiation with Sonazoid generated @entity5568 and enhanced sonoporation. ShRNA- @entity13032 suppressed cell proliferation of both cell lines at a mechanical index of 0.4. Motility of HLF cells and @entity9294 /PRF/5 cells was suppressed by shRNA- @entity13032 . Sonazoid enhanced sonoporation of the cells with the diagnostic US device and the suppression of proliferation of both @entity157 cell lines by shRNA- @entity13032 .

XXXX cell proliferation by short hairpin RNA of frizzled  2 with Sonazoid-enhanced irradiation.

@entity5568 :: ('D006861', 'Chemical') :: ['H2O2'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity13032 :: ('2535', 'Gene') :: ['FZ2', 'Fz2'] @entity6893 :: ('CHEBI:16382', 'Chemical') :: ['iodide'] @entity9294 :: ('3339', 'Gene') :: ['PLC'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hematoxylin'] @entity1495 :: ('4318', 'Gene') :: ['matrix metalloproteinase  9', 'MMP9'] @entity157 :: ('MESH:D006528', 'Disease') :: ['HCC', 'hepatocellular carcinoma']
@entity157 :: (MESH:D006528,Disease) :: ['Suppression of hepatocellular carcinoma']
