Mutagenesis assays using @entity19 have been recently developed and applied to the studies on the mutagenesis. The present study was undertaken to clarify whether the mutagenesis assay with @entity19 could detect the mutations induced by the internal beta-ray irradiation from 89Sr or the external gamma-ray irradiation. The @entity19 used were Muta @entity19 , which carries 80 copies of the bacterial lacZ gene per cell as a target of mutagenesis. Female animals were given intraperitoneal injections of @entity330 (50 mg/kg per day) for five days, a single intravenous injection of 89Sr (7.4 and 74 MBq/kg), or daily irradiation with 1.5 Gy gamma-rays for five days. The liver, spleen, and bone marrow were collected 15 days after the treatment with each agent. After the genomic DNA was extracted from each tissue, mutation analysis at lacZ locus was carried out. The spontaneous lacZ mutant frequencies were 2-4 x 10(-5). The frequencies of mutants induced by @entity330 in the liver, spleen and bone marrow were 68, 55, and 11 x 10(-5), respectively. In contrast, the mutant frequencies detected after the treatment with gamma-rays were not so high in all three tissues as those treated with @entity330 . The injection of 89Sr at a dose of 74 MBq/kg induced mutation at significantly higher frequencies in the bone marrow, but not in the liver and spleen. The results clearly showed that the mutation assay system used here could detect mutagenic effects of the local irradiation from 89Sr, but was relatively insensitive to the beta- and gamma-ray irradiation compared with the chemical mutagens such as @entity330 .

Mutant frequencies in lacZ XXXX following the internal irradiation from 89Sr or the external gamma-ray irradiation.

@entity19 :: ('10090', 'Species') :: ['transgenic mice', 'mouse strain'] @entity330 :: ('MESH:D005038', 'Chemical') :: ['ethylnitrosourea']
@entity19 :: (10090,Species) :: ['transgenic mice']
