In a population-based case-control study we have previously shown that 14% of healthy Manitobans carry one or two mutations in the @entity6820 locus, a gene highly associated with @entity30 ( @entity30 ). The @entity6820 protein is the receptor responsible for recognition of bacterial peptidoglycans, and it is plausible that @entity6820 is involved in the recognition of mycobacteria. Thirty-seven percent of Manitobans with @entity30 had >or=1 @entity6820 mutation, leading to a threefold increased risk of @entity30 for single-mutant carriers and a 30-fold increased risk for double-mutant carriers. In the same population groups, we assessed the seroprevalence for Mycobacterium @entity3068 and found it to be 35%, with no differences between @entity30 , @entity31 ( @entity31 ), and controls. Because of high rates of @entity30 and @entity31 in Manitoba, we assessed whether there was an interaction between carrying a @entity6820 mutation and M. @entity3068 seropositivity. An enzyme-linked immunosorbent assay for serum antibodies to M. @entity3068 in @entity291 was adapted for @entity1 use. DNA was purified from whole blood. Subjects were genotyped for three @entity6820 variants, @entity20717 , @entity69880 , and @entity20716 . Multivariate logistic regression analysis showed that @entity6820 gene mutations significantly associated with @entity30 , but M. @entity3068 serology did not. Furthermore, there was no interaction between @entity6820 mutation status and M. @entity3068 serology status. For those with the @entity6820 mutation, the likelihood of @entity30 subjects having positive M. @entity3068 serology was similar to that of controls (odds ratio, 1.31; 95% confidence interval, 0.55-3.11). No interaction could be proven for @entity31 or by combining @entity30 and @entity31 compared to controls. In conclusion, we could not find an interaction between the @entity6820 genotype and M. @entity3068 serology in relationship to @entity30 or @entity31 .

Testing the interaction between @entity6820 status and serological response to Mycobacterium XXXX in cases of inflammatory bowel disease.

@entity1 :: ('9606', 'Species') :: ['human'] @entity69880 :: ('c|INS|1007|C', 'ProteinMutation') :: ['Cins1007fs'] @entity31 :: ('MESH:D003093', 'Disease') :: ['ulcerative colitis', 'UC'] @entity30 :: ('MESH:D003424', 'Disease') :: ['CD', "Crohn's disease"] @entity20716 :: ('p|SUB|R|702|W', 'ProteinMutation') :: ['R702W'] @entity6820 :: ('64127', 'Gene') :: ['NOD-2'] @entity3068 :: ('MESH:D010283', 'Disease') :: ['paratuberculosis'] @entity20717 :: ('p|SUB|G|908|R', 'ProteinMutation') :: ['G908R'] @entity291 :: ('9913', 'Species') :: ['cattle']
@entity3068 :: (MESH:D010283,Disease) :: ['paratuberculosis']
