OBJECTIVE: To investigate the effect of @entity43 cell on T cell JAK-STAT signal transduction pathway and its role in the @entity43 induced immunosupression. METHODS: @entity1 @entity43 cells of OVCAR3. CAOV3, and SKOV3 lines were cultured. @entity405 (+) T cells were isolated from the peripheral venous blood of healthy @entity1 . Then the supernatants of these @entity43 cell lines and RPMI-1640 were added into the culture of @entity405 (+) T cells (groups I, II, III, and control). @entity2454 ( @entity98 ) method was used to detect the growth of @entity405 (+) T cell. The cell cycle was examined by flow cytometry. The secretion of the Tc1 type cytokine interferon (IFN)-gamma mRNA and the secretion of the Tc2 type cytokine interleukin (IL)-10 mRNA were detected by RT-PCR. The expression of signaling molecules JAK and @entity7803 and the phosphorylated activation of @entity4104 and @entity11715 in the @entity405 (+) T cell were analyzed by Western blotting. RESULTS: The absorbance at the wavelength 570 nm of @entity405 (+) T cell culture was 0.23 +/- 0.03, 0.28 +/- 0.06, and 0.29 +/- 0.05 in the group I, II, and III, all significantly lower than that in the group IV (0.79 +/- 0.07, all P < 0.01). The percentages of @entity405 (+) T cells at the stage S and stage G(2)/M were lower, and those in stage G(1)/G(0) were higher in groups I, II, and III than in group IV (all P < 0.01). The @entity379 expression was significantly lower in groups I, II, and III in comparison with that in group IV. However, the expression of @entity688 was significantly higher in groups I, II, and III in comparison with that in group IV. The expression of @entity7803 protein, but not @entity19904 protein, was significantly lower in groups I, II, and III in comparison with that in group IV. The phosphorylated activation of @entity11715 was suppressed significantly in groups I, II, and III, whereas the phosphorylated activation of @entity4104 was suppressed only in group I. CONCLUSION: @entity43 may suppress T cell proliferation through inhibition of the JAK-STAT signal transduction pathway, which may be a mechanism of @entity43 induced immunosuppression.

[ XXXX cell inhibits T cell JAK-STAT signal transduction pathway, an experimental study].

@entity43 :: ('MESH:D010051', 'Disease') :: ['ovarian carcinoma', 'Ovarian carcinoma'] @entity1 :: ('9606', 'Species') :: ['Human', 'persons'] @entity4104 :: ('6774', 'Gene') :: ['STAT3'] @entity19904 :: ('3716', 'Gene') :: ['JAK1'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity2454 :: ('MESH:C022616', 'Chemical') :: ['Thiazolyl blue'] @entity379 :: ('3458', 'Gene') :: ['IFN-gamma'] @entity7803 :: ('3718', 'Gene') :: ['JAK3'] @entity11715 :: ('6776', 'Gene') :: ['STAT5'] @entity688 :: ('3586', 'Gene') :: ['IL-10'] @entity405 :: ('925', 'Gene') :: ['CD8']
@entity43 :: (MESH:D010051,Disease) :: ['Ovarian carcinoma']
