AIM: Insufficient attention for the Ki-67 immunohistochemistry has been given to the importance of tissue handling for surgical @entity0 specimens. We sought to investigate the effect of fixation status on the Ki-67. METHODS: We examined the effect of fixative, time to and duration of fixation using surgical specimens, and finally, compared the paired Ki-67 index in the @entity5 between core needle and surgical specimen. RESULTS: The Ki-67 was significantly higher when 10% neutral buffered @entity406 was used (p=0.0276). Insufficient fixation caused a @entity353 in the Ki-67 index (p=0.0177), but not significant in @entity2931 receptor (ER) and @entity1 @entity1722 ( @entity1722 ). Sixteen hours delayed time to fixation also caused a reduction of the Ki-67 (p=0.0284), but not significant in ER. Prolonged fixation significantly led to a gradual reduction in the Ki-67 in a time-dependent manner, but not in both ER and @entity1722 . Finally, cutting the @entity5 before fixation improved fixation status and consequently caused an increased level of the Ki-67 index (p=0.0181), which resulted in a strong correlation of the Ki-67 between core needle and surgical specimen (r=0.8595). CONCLUSIONS: Tissue handling of surgical specimen is critical for assessing the Ki-67 compared with ER and @entity1722 . We should pay more attention to tissue fixation status for the standard assessment of the Ki-67 index.

The importance of tissue handling of surgically removed XXXX for an accurate assessment of the Ki-67 index.

@entity1 :: ('9606', 'Species') :: ['human'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity353 :: ('MESH:D015431', 'Disease') :: ['drastic reduction'] @entity2931 :: ('CHEBI:50114', 'Chemical') :: ['oestrogen'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumour'] @entity406 :: ('MESH:D005557', 'Chemical') :: ['formalin'] @entity1722 :: ('2064', 'Gene') :: ['epidermal growth factor receptor 2', 'HER2']
@entity0 :: (MESH:D001943,Disease) :: ['breast cancer']
