OBJECTIVE: To explore the biological functions of @entity5447 ubiquitylation-promoting complex 2 (KPC2) in the repair process of @entity1566 ( @entity1566 ) by studying the expression and cellular localization of KPC2 in @entity35 @entity1566 models. METHODS: Fifty-six adult @entity35 were randomly divided into 2 groups: in the control group (n = 7), simple T9 laminectomy was performed; in the experimental group (n = 49), the @entity1566 model was established at T9, 7 @entity35 were used to detect follow indexs at 6 hours, 12 hours, 1 day, 3 days, 5 days, 7 days, and 14 days after SC. Western blot analysis was used to detect the protein expressions of @entity5447 , KPC2, CyclinA and @entity1975 ( @entity1975 ) after @entity1566 . Immunohistochemistry was used to observed the cellular localization of KPC2 after @entity1566 , double-labeling immunofluorescence staining to observe the co-localization of KPC2 with @entity2141 ( @entity2141 ), @entity40 ( @entity40 ) and @entity1975 . In vitro astrocytes proliferation model was used to further validate these results, Western blot to detect KPC2, @entity5447 , and @entity1975 expressions. The interaction of @entity5447 , KPC1, and KPC2 in cell proliferation was analyzed by co-immunoprecipitation. RESULTS: The Western blot analysis showed a significant down-regulation of @entity5447 and a concomitant up-regulation of KPC2, CyclinA, and @entity1975 after SC. Immunohistochemistry staining revealed a wide distribution of KPC2 positive signals in the gray matter and white matter of the spinal cord. The number of KPC2 positive cells in the experimental group was significantly higher than that in the control group (t = 10.982, P = 0.000). Double-labeling immunofluorescence staining revealed the number of KPC2/ @entity2141 co-expression cells in the gray matter of spinal cord was (0.43    0.53)/visual field in the control group and (0.57    0.53)/visual field in the experimental group, showing no significant difference (t = 0.548, P = 0.604); in the white matter of spinal cord, the number of KPC2/ @entity1975 co-expression cells was (3.86    0.90)/visual field in the control group and (0.71    0.49)/visual field in the experimental group, showing significant difference (t = 7.778, P = 0.000). And then, the number of KPC2/ @entity1975 co-expression cells were (0.57    0.53)/visual field in the control group and (5.57    1.13)/visual field in the experimental group, showing significant difference (t = 8.101, P = 0.000). Concomitantly, there was a similar kinetic in proliferating astrocytes in vitro. The Western blot analysis showed a significant down-regulation of @entity5447 and a concomitant up-regulation of KPC2 and @entity1975 after serum stimulated. Co-immunoprecipitation demonstrated increased interactions between @entity5447 , KPC1, and KPC2 after stimulation. CONCLUSION: The up-regulated expression of KPC2 after @entity1566 is related to the down-regulation of @entity5447 , this event may be involved in the proliferation of astrocytes after @entity1566 .

[EXPRESSION AND ROLE OF @entity5447 UBIQUITYLATION-PROMOTING COMPLEX 2 IN ASTROCYTES AFTER SPINAL CORD INJURY IN XXXX ].

@entity35 :: ('10116', 'Species') :: ['rat', 'Sprague-Dawley rats', 'rats'] @entity2141 :: ('287847(Tax:10116)', 'Gene') :: ['neuronal nuclei', 'NeuN'] @entity1975 :: ('25737(Tax:10116)', 'Gene') :: ['PCNA', 'proliferating cell nuclear antigen'] @entity40 :: ('24387(Tax:10116)', 'Gene') :: ['GFAP', 'glial fibrillary acidic protein'] @entity1566 :: ('MESH:D013119', 'Disease') :: ['spinal cord injury', 'SCI'] @entity5447 :: ('83571(Tax:10116)', 'Gene') :: ['P27kip1', 'Kip1', 'p27kip1']
@entity35 :: (10116,Species) :: ['RATS']
