OBJECTIVES: @entity1301 (NP) is present ubiquitously in both aquatic and terrestrial environments. This compound is considered an important endocrine disruptor and its toxic/oestrogenic activity has been investigated in a number of in vitro and in vivo studies. @entity1 exposure to NP may occur by cutaneous absorption, ingestion of contaminated food or water and inhalation. Moreover, while the cytotoxic effects of NP are known and studied, its effects on cell death and related mechanisms are not known. Our group decided to investigate NP effects on @entity356 ( @entity15206 ), in particular NP effects on @entity15206 cell cycle and apoptosis. MATERIALS AND METHODS: Cell cycle was analysed by flow cytometry, @entity3544 and @entity993 induction, and apoptosis was analysed by flow cytometry and @entity4404 assays, Fas, @entity12 , @entity8186 and @entity522 activation. RESULTS: We have demonstrated that NP affected cell cycle and apoptosis in a time- and dose-dependent manner, reaching the most notable effect at concentration of 10(-7)m, for 48 h. Flow cytometry revealed that treatment with 10(-7) m NP led to accumulation of cells at G(2) /M transition and increased percentage population of apoptotic cells. Results have shown that NP at concentration 10(-7) m induced marked increase in Fas and @entity12 expression. Moreover, 10(7) m NP induced activation of caspases 8 and 3, but not activation of @entity8187 . CONCLUSIONS: The results reported suggest that gastric cells can be useful as screening population for appropriate study of developmental @entity137 .

Effects of 4-nonylphenol on proliferation of XXXX gastric cells.

@entity1 :: ('9606', 'Species') :: ['Human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity522 :: ('836', 'Gene') :: ['caspase 3'] @entity4404 :: ('308', 'Gene') :: ['annexin-V'] @entity993 :: ('10671', 'Gene') :: ['p27'] @entity3544 :: ('644914', 'Gene') :: ['p21'] @entity8186 :: ('841', 'Gene') :: ['caspase 8'] @entity8187 :: ('842', 'Gene') :: ['caspase 9'] @entity12 :: ('356', 'Gene') :: ['Fas-L'] @entity1301 :: ('MESH:C025256', 'Chemical') :: ['Nonylphenol'] @entity15206 :: ('MESH:C535607', 'Disease') :: ['AGS'] @entity356 :: ('MESH:D013274', 'Disease') :: ['a gastric epithelial cell line']
@entity15206 :: (MESH:C535607,Disease) :: ['AGS']
