Two subtypes of the vesicular @entity2227 transporter are expressed differentially in two excitatory afferents synapsing on to Purkinje cells: @entity18358 (BNPI) in axon terminals of cerebellar granule cells (i.e. parallel fibres; PFs) and @entity53793 ( @entity53793 ) in those of the inferior olivary neurons (climbing fibres; CFs). In the present study, we examined their expression in the developing @entity19 cerebellum. By in situ hybridization, the inferior olivary nucleus selectively expressed @entity53793 mRNA through postnatal life. In the cerebellum, both subtypes were transcribed in the external and internal granular layers during the first postnatal week. Thereafter, @entity18358 mRNA showed marked upregulation in the internal granular layer, whereas @entity53793 mRNA disappeared from the external and internal granular layers by the end of the third postnatal week. By immunohistochemistry, @entity1577 terminals consistently exhibited @entity53793 immunoreactivity in the postnatal cerebellum. By contrast, in the first 10 days of postnatal life, @entity53793 predominated in PF terminals, despite the transcription of both transporters in developing granule cells. During the second 10 days, @entity53793 in PF terminals was replaced with @entity18358 from deep regions of the molecular layer upwards, correlating with @entity27324 . This replacement was accomplished by postnatal day 30. Taking that late-borne PFs are laid down successively on earlier ones in the molecular layer, the deep-to-superficial replacement represents maturation-linked switching from @entity53793 to @entity18358 in individual PFs, and is likely to be regulated at both the transcription and translation levels.

Subtype switching of vesicular @entity2227 transporters at parallel fibre-Purkinje cell synapses in developing XXXX cerebellum.

@entity2227 :: ('CHEBI:29987', 'Chemical') :: ['glutamate'] @entity53793 :: ('140919(Tax:10090)', 'Gene') :: ['DNPI', 'VGluT2'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity27324 :: ('MESH:D014178', 'Disease') :: ['dendritic translocation of CFs'] @entity18358 :: ('72961(Tax:10090)', 'Gene') :: ['VGluT1'] @entity1577 :: ('MESH:D003550', 'Disease') :: ['CF']
@entity19 :: (10090,Species) :: ['mouse']
