UNASSIGNED: The dire effects of @entity5 -induced @entity3085 undermine treatment and contribute to decreased survival rates. Therapeutic options for this syndrome are limited, and therefore efforts to identify signs of precachexia in @entity5 @entity1 are necessary for early intervention. The applications of molecular and functional imaging that would enable a whole-body "holistic" approach to this problem may lead to new insights and advances for diagnosis and treatment of this syndrome. Here we have developed a myoblast optical reporter system with the purpose of identifying early cachectic events. We generated a myoblast cell line expressing a dual tdTomato:GFP construct that was grafted onto the muscle of @entity19 -bearing @entity1 @entity788 xenografts to provide noninvasive live imaging of events associated with @entity5 -induced @entity3085 (i.e., @entity353 ). Real-time optical imaging detected a strong tdTomato fluorescent signal from skeletal muscle grafts in @entity19 with weight losses of only 1.2% to 2.7% and @entity5 burdens of only approximately 79 to 170 mm(3). Weight loss in cachectic animals was also associated with a depletion of lipid, @entity165 , @entity4857 , and @entity3929 levels, which may provide informative biomarkers of @entity3085 . Taken together, our findings demonstrate the utility of a reporter system that is capable of tracking @entity5 -induced @entity353 , an early marker of @entity3085 . Future studies incorporating resected tissue from @entity1 @entity1124 into a reporter-carrying @entity19 may be able to provide a risk assessment of @entity3085 , with possible implications for therapeutic development. @entity5 Res; 76(6); 1-10.   2015 AACR.

Detection of XXXX Using a Fluorescent Myoblast Reporter System and Analysis of Metabolite Abundance.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity788 :: ('MESH:D010190', 'Disease') :: ['pancreatic cancer'] @entity353 :: ('MESH:D015431', 'Disease') :: ['weight loss'] @entity1124 :: ('MESH:D021441', 'Disease') :: ['pancreatic ductal adenocarcinoma'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'Cancer'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity3085 :: ('MESH:D002100', 'Disease') :: ['cachexia'] @entity3929 :: ('D000409', 'Chemical') :: ['alanine'] @entity4857 :: ('CHEBI:27266', 'Chemical') :: ['valine'] @entity165 :: ('MESH:D002784', 'Chemical') :: ['cholesterol']
@entity788 :: (MESH:D010190,Disease) :: ['Pancreatic Cancer-Induced Cachexia']
