BACKGROUND/PURPOSE: Antiangiogenic agents offer a new approach to the treatment of aggressive @entity5 , yet very few agents are available for current use. The authors have shown previously the efficacy of antiangiogenic therapy in experimental @entity3969 , using an investigative antibody. They hypothesized that @entity1945 , administered in a regimen targeting endothelial cells, would suppress @entity5 growth and @entity7499 in experimental @entity3969 . METHODS: Experimental @entity5 were induced in the left kidneys of athymic @entity19 by injection of cultured @entity3969 cells. Topotecan (0.36, 0.6, 1.0, 2.0, and 3.0 mg/kg) or vehicle was injected intraperitoneally in 2 cycles over a 6-week period. Fluorescein angiograms and platelet endothelial cell adhesion molecule-1 staining of @entity5 were performed to ascertain vascular architecture. Endothelial apoptosis was assessed by @entity23929 -mediated @entity1977 nick end labeling assay. RESULTS: @entity5 weights were reduced significantly in treated versus control animals, even in the lowest-dose group. Endothelial cell staining and angiography results showed relatively sparse vascularity in treated xenografts. Endothelial apoptosis was observed in treated but not control @entity5 . CONCLUSIONS: Topotecan, delivered in an "antiangiogenic" regimen, even at very low doses, significantly inhibited growth of experimental @entity3969 . No adverse effects were noted at low doses. Thus, the established chemotherapy agent topotecan may be useful in a novel role: as antiangiogenic therapy. J Pediatr Surg 36:1781-1784.

Novel use of an established agent: Topotecan is anti-angiogenic in experimental XXXX .

@entity7499 :: ('MESH:D016510', 'Disease') :: ['angiogenesis'] @entity23929 :: ('21673(Tax:10090)', 'Gene') :: ['TdT'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'primary tumors', 'Tumor', 'neoplasms', 'tumors'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity1977 :: ('CHEBI:17625', 'Chemical') :: ['dUTP'] @entity3969 :: ('MESH:D009396', 'Disease') :: ['Wilms tumors', 'Wilms tumor'] @entity1945 :: ('MESH:D019772', 'Chemical') :: ['topotecan']
@entity3969 :: (MESH:D009396,Disease) :: ['Wilms tumor']
