Theiler's @entity19 @entity1651 virus (TMEV) is a positive-sense RNA virus belonging to the Cardiovirus genus in the family Picornaviridae. In addition to other host cellular factors and pathways, picornaviruses utilise @entity1457 proteins (Hsps) to facilitate their propagation in cells. This study investigated the localisation of Hsps 70 and 90 in TMEV-infected BHK-21 cells by indirect immunofluorescence and confocal microscopy. The effect of @entity23118 inhibitors @entity8801 (Nov) and @entity13906 (GA) on the development of cytopathic effect (CPE) induced by @entity281 was also examined. @entity23118 staining was uniformly distributed in the cytoplasm of uninfected cells but was found concentrated in the perinuclear region during late @entity281 where it overlapped with the signal for non-structural protein 2C within the viral replication complex. @entity187 redistributed into the vicinity of the viral replication complex during late @entity281 , but its distribution did not overlap with that of 2C. Inhibition of @entity23118 by GA and Nov had a negative effect on virus growth over a 48-h period as indicated by no observable CPE in treated compared to untreated cells. 2C was detected by Western analysis of GA-treated infected cell lysates at doses between 0.01 and 0.125   M, suggesting that processing of viral precursors was not affected in the presence of this drug. In contrast, 2C was absent in cell lysates of Nov-treated cells at doses above 10   M, although CPE was evident 48  hpi. This is the first study describing the dynamic behaviour of Hsps 70 and 90 in TMEV-infected cells and to identify @entity23118 as an important host factor in the life cycle of this virus.

Theiler's @entity19 @entity1651 virus XXXX induces a redistribution of @entity1457 proteins 70 and 90 in BHK-21 cells, and is inhibited by @entity8801 and @entity13906 .

@entity23118 :: ('111058(Tax:10090)', 'Gene') :: ['Hsp90'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity1457 :: ('MESH:D012769', 'Disease') :: ['heat shock'] @entity13906 :: ('MESH:C001277', 'Chemical') :: ['geldanamycin'] @entity8801 :: ('MESH:D009675', 'Chemical') :: ['novobiocin'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity187 :: ('15511(Tax:10090)', 'Gene') :: ['Hsp70'] @entity1651 :: ('MESH:D004679', 'Disease') :: ['encephalomyelitis']
@entity281 :: (MESH:D007239,Disease) :: ['infection']
