INTRODUCTION: Resistance to Carbapenems in Klebsiella may be due to @entity2893 hydrolysing enzymes. Accurate detection of carbapenemase must be done for @entity1 treatment and epidemiological purposes. AIM: To detect carbapenemase production by performing Modified Hodge Test (MHT), @entity1062 ( @entity1062 ) for @entity23041 ( @entity23041 ) and PCR for blaKPC gene, to evaluate the performance of MHT using MacConkey Agar (MCA) and to access the value of MHT for carbapenemase detection. MATERIAL AND METHODS: Using a prospective laboratory study design, 153 Extended Spectrum Beta-Lactamases (ESBL) producing @entity3274 from clinical samples of @entity1 admitted in the Kasturba Medical College were collected from January 2014 to December 2015. Isolates resistant to carbapenems by disk diffusion were subjected to MHT on MCA and @entity23977 ( @entity23977 ). All isolates were tested for ( @entity23041 ) production by Imipenem and Imipenem-EDTA @entity1062 and subjected to PCR for the presence of blaKPC gene. RESULTS: Out of 153 isolates, 54 were resistant to one of the carbapenems. Among these, 13 were positive for MHT on @entity23977 , while 23 were positive by MHT on MCA. Number of @entity23041 producers was 23 (42.5%), while blaKPC was detected in 2 out of the 54 isolates. CONCLUSION: Though detection of drug resistance gene remains the method of choice, it can be performed only in centers with adequate resources. Hence, for most laboratories in resource poor countries, the MHT performed on MCA with concomitant @entity1062 for @entity23041 detection seem to be a better option for detection of @entity2893 resistance.

Approach to Carbapenemase Detection in XXXX in Routine Diagnostic Laboratories.

@entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity23977 :: ('MESH:C535871', 'Disease') :: ['MHA', 'Mueller Hinton agar'] @entity23041 :: ('MESH:C535600', 'Disease') :: ['MBL', 'Metallo-b-Lactamases'] @entity3274 :: ('573', 'Species') :: ['Klebsiella pneumoniae'] @entity2893 :: ('CHEBI:46765', 'Chemical') :: ['Carbapenem'] @entity1062 :: ('MESH:D013736', 'Disease') :: ['CDT', 'Combined Disk Test']
@entity3274 :: (573,Species) :: ['Klebsiella pneumoniae']
