We examined the effects of dissolved @entity842 (NO) gas on cytoplasmic @entity460 levels ([ @entity141 ](i)) in C6 @entity11 cells under anoxic conditions. The maximum elevation (27 +/- 3 nM) of [ @entity141 ](i) was reached at 10 microM NO. A second application of NO was ineffective if the first was >0.5 microM. The NO donor @entity21677 /NO mimicked the effects of NO. Acute exposure of the cells to low @entity460 levels was without effect on the NO-evoked response. @entity2426 (TG) increased [ @entity141 ](i) and was less effective if cells were pretreated with NO. @entity54413 inhibited the effects of NO at a molar ratio of 10:1. @entity8263 was without effect on the NO-evoked response. If cells were pretreated with TG or exposed chronically to nominal amounts of @entity460 , NO decreased [ @entity141 ](i). The results suggest that C6 @entity11 cells have two receptors for NO. One receptor (NO(A)) elevates [ @entity141 ](i) and resides on the endoplasmic reticulum (ER). The other receptor ( @entity32737 ) decreases [ @entity141 ](i) and resides on the plasmalemma or the ER. The latter receptor dominates when the level of @entity460 within intracellular stores is diminished.

Regulation of cytoplasmic @entity460 levels by two XXXX receptors.

@entity2426 :: ('MESH:D019284', 'Chemical') :: ['Thapsigargin'] @entity32737 :: ('CHEBI:27986', 'Chemical') :: ['NO(B)'] @entity21677 :: ('MESH:C034281', 'Chemical') :: ['diethylamine'] @entity11 :: ('MESH:D005910', 'Disease') :: ['glioma'] @entity8263 :: ('MESH:C016276', 'Chemical') :: ['8-Bromo-cGMP'] @entity54413 :: ('CHEBI:5656', 'Chemical') :: ['Hemoglobin'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)']
@entity842 :: (MESH:D009569,Chemical) :: ['nitric oxide']
