UNASSIGNED: Current vaccination is effective against encapsulated strains, but do not protect against nonencapsulated @entity328 ( @entity60263 ), which are increasing in both colonization and @entity66 . Vaccination with pneumococcal proteins has been assessed for the ability to protect against @entity66 , but several of these proteins are not expressed by @entity60263 . PspK, a @entity60263 virulence factor, has not been assessed for immunogenic potential or host modulatory effects. @entity1 cytokine expression was determined in both a @entity19 in vivo model as well as in an in vitro cell culture system. Systemic and mucosal @entity19 immunization studies were performed to determine immunogenic potential of PspK. Murine serum and saliva was collected to quantitate specific antibody isotype response as well as the ability of antibody and various proteins to inhibit epithelial cell adhesion. Host cytokine response was not reduced by PspK. @entity60263 were able to colonize the @entity19 nasopharynx as effectively as encapsulated pneumococci. Systemic and mucosal immunization provided protection from colonization by PspK(+) @entity60263 . Anti-PspK antibodies were recovered from immunized @entity19 and significantly reduced the ability of @entity60263 to adhere to @entity1 epithelial cells. A protein based pneumococcal vaccine is needed to provide broad protection against both encapsulated and nonencapsulated pneumococci in an era of increasing antibiotic resistance and vaccine escape mutants. We demonstrate that PspK may serve as a @entity60263 target for next generation pneumococcal vaccines. Immunization with PspK protected against pneumococcal colonization, which is requisite for @entity66 .

Immunization with Pneumococcal Surface Protein K of Nonencapsulated XXXX Provides Protection in a @entity19 Model of Colonization.

@entity1 :: ('9606', 'Species') :: ['human', 'Mammalian'] @entity60263 :: ('18008(Tax:10090)', 'Gene') :: ['NESp'] @entity66 :: ('MESH:D004194', 'Disease') :: ['pneumococcal disease'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity328 :: ('1313', 'Species') :: ['Streptococcus pneumoniae']
@entity328 :: (1313,Species) :: ['Streptococcus pneumoniae']
