BACKGROUND @entity2132 ( @entity2132 ) is the major cause of morbidity and mortality after stem cell transplantation. The development of early prediction methods is therefore of importance. Our aim was to analyze the usefulness of early donor chimerism monitoring after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in T cells and in @entity404 + and @entity405 + (lineage chimerism) for @entity2132 prediction. MATERIAL AND METHODS Chimerism was analyzed in 76 consecutive adult @entity1 using RQ-PCR TaqMan technology on DNA extracted from Pan T, @entity404 +, and @entity405 + cell subsets on Day 5, 10, 15 and 30 after allo-HSCT. RESULTS The threshold of chimerism predictive for @entity2132 was the same for all tested cell subsets. In @entity3052 ( @entity3052 ) @entity1 treated with myeloablative conditioning (MAC), the threshold predictive for @entity2132 was 95% and 99% for Day 10 and Day 15, respectively. In @entity1 treated with reduced intensity conditioning (RIC), the threshold predictive for chronic graft versus host disease was 98% on Day 10. The differences were statistically significant. CONCLUSIONS Chimerism analysis in T cell subsets by RQ-PCR on Day 10 and Day 15 after transplantation is useful for prediction of aGvHD ( @entity3052 @entity1 after MAC) and cGvHD ( @entity1 after RIC). However, there was no difference in the results between chimerism in the T cell subsets. Our RQ-PCR protocol was highly sensitive and proved effective for analysis of lineage chimerism.

Real-Time PCR Analysis of Chimerism in T Cell Subsets as an Early Predictor of XXXX Following Allogeneic Stem Cell Transplantation.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity404 :: ('920', 'Gene') :: ['CD4'] @entity3052 :: ('MESH:D015470', 'Disease') :: ['acute myeloid leukemia', 'AML'] @entity2132 :: ('MESH:D006086', 'Disease') :: ['Graft-versus-host-disease', 'GvHD', 'acute graft versus host disease'] @entity405 :: ('925', 'Gene') :: ['CD8']
@entity2132 :: (MESH:D006086,Disease) :: ['Graft-Versus-Host Disease']
