We investigated, in a @entity95 model, the effects of castration and @entity251 replacement on: 1) the hemodynamics of the corpus cavernosum; 2) alpha-1 adrenergic receptor protein expression; 3) neural NO synthase protein expression and activity; 4) phosphodiesterase type 5 activity; and 5) trabecular smooth muscle/connective tissue balance. One week after bilateral orchiectomy, animals were treated for 7 days with vehicle alone, @entity251 , or @entity53 . Intact control animals received vehicle only. Systemic arterial blood and @entity551 ( @entity551 ) were measured in each animal before and after electrical stimulation of the cavernosal nerve. Alpha1-adrenergic receptor protein expression was determined by ligand binding studies. NO synthase expression and activity were determined by Western blot analyses and conversion of @entity534 to @entity7792 , respectively. Phosphodiesterase type 5 activity was determined by hydrolysis of @entity6708 ( @entity6708 ) in tissue extracts in the absence or presence of 100 nM @entity7855 . Smooth muscle content was assessed by Masson's trichrome staining and computer-assisted histomorphometry. Castration significantly reduced @entity551 , but it did not alter systemic arterial blood pressure during stimulation of the cavernosal nerve. @entity251 , but not @entity53 , treatment prevented the effects of castration and @entity551 to values similar to those obtained in intact animals. Castration reduced expression of alpha1-adrenergic receptor, and this reduction was prevented or reversed by @entity251 replacement. Neural NO synthase protein expression and total activity were not altered significantly by castration or after @entity251 replacement. However, phosphodiesterase type 5 activity increased in castrated animals treated with @entity251 . Castration significantly reduced trabecular smooth muscle content, and this reduction was restored by @entity251 (but not @entity53 ) treatment. The results of this study demonstrate that @entity342 deprivation alters the functional responses and structure of erectile tissue.

Effects of castration and XXXX replacement on erectile function in a @entity95 model.

@entity7792 :: ('CHEBI:16349', 'Chemical') :: ['citrulline'] @entity6708 :: ('CHEBI:16356', 'Chemical') :: ['cGMP', "guanosine 3',5'-cyclic monophosphate"] @entity251 :: ('MESH:D013739', 'Chemical') :: ['testosterone', 'Testosterone'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity534 :: ('CHEBI:16467', 'Chemical') :: ['L-arginine'] @entity53 :: ('MESH:D004958', 'Chemical') :: ['estradiol'] @entity7855 :: ('MESH:C101426', 'Chemical') :: ['sildenafil'] @entity342 :: ('CHEBI:50113', 'Chemical') :: ['androgen'] @entity551 :: ('MESH:D007022', 'Disease') :: ['intracavernosal pressures', 'restored ICP', 'ICP']
@entity342 :: (CHEBI:50113,Chemical) :: ['androgen']
