OBJECTIVE: To repair experimental osteochondral knee lesions in @entity1707 using tissue engineering. MATERIAL AND METHODS: Eight 40-kg @entity1707 underwent surgery. Cartilage and periosteal biopsies of their control knee were taken. Cartilage and periosteal cells were independently isolated, cultured and seeded in biodegradable @entity6987 and @entity1989 polymers that were fixed on the bottom of an @entity4713 in the @entity1707 's experimental knee, with bioabsorbable Mitek implants. Four months later the @entity1707 were sacrificed and the knees were analyzed with nuclear magnetic resonance imaging (NMRI), macroscopic assessment, histology, electron microscopy (EM), @entity4653 ( @entity4653 ) and @entity4653 element analysis. RESULTS: All the defects were filled with cartilage-like tissue according to the NMRI evaluation and the visual examination. Hyaline-like cartilage was obtained in 3 defects and fibrocartilage in 5. The EM showed chondrocytes in the repair tissue. The @entity4653 showed appropriate integration to the bone and the surrounding tissue. @entity4653 element analysis showed sulphurized matrix attached to the bone with @entity460 and @entity6546 as predominant elements. DISCUSSION: Tissue engineering enabled the production of tissues similar to normal ones. The polymer fixation system was effective.

[Results of the experimental repair of osteochondral lesions in a XXXX model using tissue engineering].

@entity1707 :: ('9823', 'Species') :: ['pigs', 'pig'] @entity6987 :: ('MESH:D011454', 'Chemical') :: ['PGA'] @entity4713 :: ('MESH:D010007', 'Disease') :: ['osteochondral defect'] @entity6546 :: ('MESH:D010710', 'Chemical') :: ['phosphates'] @entity1989 :: ('CHEBI:60159', 'Chemical') :: ['PLA'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity4653 :: ('MESH:D004401', 'Disease') :: ['SEM', 'scanning electron microscopy']
@entity1707 :: (9823,Species) :: ['pig']
