Although surgery remains the mainstay of curative treatment for @entity14 ( @entity14 ), many @entity1 still have high chance to experience disease relapse. It is therefore imperative to identify prognostic markers that can help predict the clinical outcomes of @entity14 . Aberrant microRNA expression holds great potential as diagnostic and prognostic biomarker for @entity14 . Here we aimed to investigate clinical potential of miR-34a-5p as a prognostic marker for @entity14 recurrence and its functional significance. First, we validated that miR-34a-5p was downregulated in @entity14 tissues (P<0.05). The expression level of tissue miR-34a-5p was then evaluated in two independent cohorts of 268 @entity14 @entity1 . miR-34a-5p expression was positively correlated with disease-free survival in two independent cohorts (cohort I: n=205, P<0.001; cohort II: n=63, P=0.006). Moreover, the expression of miR-34a-5p was an independent prognostic factor for @entity14 recurrence by multivariate analysis (P<0.001 for cohort I, P=0.007 for cohort II). Ectopic expression of miR-34a-5p in @entity876 wild-type @entity694 cell HCT116 significantly inhibited cell growth, migration, invasion and @entity3 . miR-34a-5p induced cell apoptosis, cell cycle arrest at G1 phase and @entity876 transcription activity in HCT116 cells, but not in the HCT116 @entity876 knockout ( @entity876 (-/-)) cells. miR-34a-5p significantly suppressed the HCT116 growth in vivo, whereas it showed no effect on the HCT116 @entity876 (-/-) xenograft, indicating that the growth-inhibiting effect by miR-34a-5p was dependent on @entity876 . In addition, the expression level of miR-34a-5p in @entity1 with @entity876 -positive expression was higher than that in @entity1 with @entity876 -negative expression (P<0.01). In conclusion, miR-34a-5p inhibits recurrence of @entity14 through inhibiting cell growth, migration and invasion, inducing cell apoptosis and cell cycle arrest in a @entity876 -dependent manner.Oncogene advance online publication, 3 November 2014; doi:10.1038/onc.2014.348.

miR-34a-5p suppresses @entity14 @entity3 and predicts recurrence in XXXX with @entity14 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity14 :: ('MESH:D015179', 'Disease') :: ['CRC', 'colorectal cancer', 'CRC tumour'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon cancer']
@entity1 :: (9606,Species) :: ['patients']
