PURPOSE: To investigate the prognostic significance of apoptosis in granulosa cells recovered from @entity1 participating in IVF-ET. METHODS: Seventy-three @entity1 underwent @entity43 for IVF and embryo transfer. After follicle aspiration, recovered granulosa cells were stained by @entity588 monoclonal antibody with FITC and @entity2667 (PI). Fluorescence was detected by flowcytometry, then the apoptotic index (Al) and @entity588 positivity were assessed. RESULTS: The pregnant group showed a significantly lower apoptotic index in granulosa cells compared with the nonpregnant group (p < 0.0001). @entity1 's age, basal serum FSH concentration, serum E2 concentrations at hCG day, number of retrieved oocytes, fertilization rates. number of embryos transferred, and @entity588 positivity were not different between the two groups. Linear regression analysis of AI to serum basal FSH showed a positive correlation. Al in granulosa cells of 6.14% or below could predict a successful pregnancy with a sensitivity of 87.5% and a specificity of 73.7%. CONCLUSION: Our results indicate that apoptotic analysis within granulosa cells can be used as a prognostic indicator for IVF success.

Prognostic implication of apoptosis in XXXX luteinized granulosa cells during IVF-ET.

@entity1 :: ('9606', 'Species') :: ['women', 'patients', 'Patient'] @entity43 :: ('MESH:D010051', 'Disease') :: ['ovarian hyperstimulation'] @entity588 :: ('596', 'Gene') :: ['BCL2'] @entity2667 :: ('MESH:D011419', 'Chemical') :: ['propidium iodide']
@entity1 :: (9606,Species) :: ['human']
