According to the @entity5 stem cells (CSCs) theory, @entity5 may be heterogeneous in which a small population of CSCs drive the progression of @entity5 . Because of their intrinsic abilities, CSCs may survive a variety of treatments and then lead to therapeutic resistance and @entity5 recurrence. Pancreatic CSCs have been reported to be responsible for the @entity788 , including suppression of immune protection. Thus, development of immune strategies to eradicate pancreatic CSCs may be of great value for the treatment of @entity788 . In this study, we enriched pancreatic CSCs by culturing Panc-1 cells under sphere-forming conditions. Panc-1 CSCs expressed low levels of HLA-ABC and @entity2937 , as measured by flow cytometry analysis. We further found that the Panc-1 CSCs modulate immunity by inhibiting lymphocyte proliferation which is promoted by phytohemagglutinin (PHA) and anti-CD3 monoclonal antibodies. The monocyte derived dendritic cells (DCs) were charged with total lysates generated from Panc-1 CSCs obtained from @entity5 sphere culturing. After co-culturing with lymphocytes at different ratios, the Panc-1 CSCs lysates modified DC effectively promoted lymphocyte proliferation. The activating efficiency reached 72.4% and 74.7% at the ratios of 1   10 and 1   20 with lymphocytes. The activated lymphocytes secreted high levels of INF-y and @entity1199 , which are strong antitumor cytokines. Moreover, Panc-1 CSCs lysates modified DC induced significant cytotoxic effects of lymphocytes on Panc-1 CSCs and parental Panc-1 cells, respectively, as shown by @entity880 dehydrogenase (LDH) assay. Our study demonstrates that the development of CSCs-based vaccine is a promising strategy for treating @entity788 .

Dendritic Cells Loaded with XXXX Stem Cells (CSCs) Lysates Induce Antitumor Immune Killing Effect In Vitro.

@entity788 :: ('MESH:D010190', 'Disease') :: ['pancreatic cancer', 'malignant behaviors of pancreatic cancer'] @entity2937 :: ('942', 'Gene') :: ['CD86'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'malignant tumors', 'cancer'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity1199 :: ('3558', 'Gene') :: ['IL-2']
@entity788 :: (MESH:D010190,Disease) :: ['Pancreatic Cancer']
