@entity9925 ) is on the verge of global eradication. Due to asymptomatic shedding, eradication certification requires environmental and clinical surveillance. Current environmental surveillance methods involve collection and processing of 400-mL to 1-L grab samples by a two-phase separation method, where sample volume limits detection sensitivity. Filtration of larger sample volumes facilitates increased detection sensitivity. This study describes development of a pumpless in-field filtration system for @entity9925 recovery from environmental waters. Recovery of PV types 1, 2, and 3 were compared for glass wool, ViroCap, and NanoCeram ( @entity37190 only) filters. Seeded experiments were performed using 10(5) plaque forming units of PV inoculated into 10-L volumes of secondary effluent, surface water, or a 50:50 mixture of each at pH 7.0. Filter eluates were plated onto buffalo @entity3176 kidney cells for virus enumeration by plaque assay. Across all water types, recovery from glass wool filters for @entity37190 , PV2, and PV3 averaged 17%, 28%, and 6%, respectively. Recovery from ViroCaps for @entity37190 , PV2, and PV3 averaged 44%, 70%, and 81%, respectively. 10-L samples of moderate turbidity water were processed through ViroCap filters in less than 30 minutes using a pumpless, bag-mediated filtration system. Bag-mediated filtration offers a simple, compact, and efficient method for enhanced environmental PV surveillance.

Development of a novel bag-mediated filtration system for environmental recovery of XXXX .

@entity37190 :: ('83483', 'Gene') :: ['PV1'] @entity3176 :: ('9534', 'Species') :: ['green monkey'] @entity9925 :: ('138950', 'Species') :: ['poliovirus', 'Poliovirus (PV']
@entity9925 :: (138950,Species) :: ['poliovirus']
