BACKGROUND AND AIMS: Ischemic preconditioning (IPC) protects tissues against @entity63 and reperfusion (I/R) injury. The aim of this study was to examine the impact of IPC on protection and regeneration of hepatocytes after prolonged I/R injury. METHODS: A @entity35 model of segmental (70%) @entity99 was used to determine the effect of 10-min IPC preceding 40, 60, 90, or 120 min of @entity409 . The effect was assessed by comparing cytolysis markers and @entity39 areas of the liver, as well as the regenerative capacity of hepatocytes using the proliferating cell nuclear antigen labeling index (PCNA-LI) and weight of the @entity2806 . Protein kinase B/ @entity2308 ( @entity2308 ) and @entity2311 were investigated immunohistochemically to determine the effect of IPC on activation of survival and anti-apoptotic signals. RESULTS: In the model of 40 min I/R, which resulted in focal @entity39 of the liver, IPC significantly protected against I/R injury by reducing the area of focal @entity39 , level of PCNA-LI and immunoreactivities to @entity2308 and @entity2311 . In contrast, IPC did not prevent @entity2806 in the 90- and 120-min @entity63 model with massive @entity409 . However, IPC enhanced the regenerative capacity of the remaining hepatocytes with higher levels of PCNA-LI, number of @entity2308 -positive cells and mean weight of the liver lobe postoperatively than in the controls. CONCLUSIONS: In a model of focal @entity39 of the liver, IPC protected hepatocytes against I/R injury. In addition, in a model of @entity11659 , IPC maintained the regenerative capacity of the remaining hepatocytes by enhancing the survival signals.

Ischemic preconditioning enhances regenerative capacity of hepatocytes in long-term ischemically damaged XXXX livers.

@entity2308 :: ('24185(Tax:10116)', 'Gene') :: ['Akt'] @entity2806 :: ('MESH:D002545', 'Disease') :: ['ischemic liver lobe', 'ischemic damage'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrotic', 'necrosis'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemia', 'ischemic'] @entity409 :: ('MESH:D008107', 'Disease') :: ['liver ischemia', 'liver necrosis'] @entity2311 :: ('58918(Tax:10116)', 'Gene') :: ['caspase-9'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity99 :: ('MESH:D056486', 'Disease') :: ['hepatic ischemia'] @entity11659 :: ('MESH:D047508', 'Disease') :: ['massive necrosis']
@entity35 :: (10116,Species) :: ['rat']
