BACKGROUND/AIMS: Our previous studies demonstrated enhanced neutrophil chemotaxis in bile duct-ligated, @entity3883 @entity35 . In the present study, we produced a reversible @entity3883 model in @entity35 . The efficacy of the present model in producing sufficient bile flow blockade and subsequent internal biliary drainage was assessed. Furthermore, the effect of internal biliary drainage on neutrophil chemotaxis was evaluated. METHODOLOGY: Bile duct was obstructed with a @entity2635 tape attached with a stainless steel coil. Internal biliary drainage was performed by removing the tape. @entity35 were subjected to either 10 days' @entity1448 or 4 days' @entity1448 followed by 6 days' internal biliary drainage. Some animals underwent conventional bile duct ligation and dissection for 4 or 10 days. Neutrophil chemotaxis was evaluated with a modified Boyden method using interleukin-8 (recombinant @entity35 Gro-beta) as chemoattractant. RESULTS: The present technique produced @entity3883 as evidenced by increases in serum @entity493 aminotransferase and total @entity648 throughout the observation period, the values of which were insignificant with those induced by the conventional method. Internal biliary drainage effectively normalized these values. Similarly, neutrophil chemotaxis was enhanced with both procedures, and increased neutrophil chemotaxis was significantly decreased after drainage. CONCLUSIONS: The present reversible @entity3883 method is as efficacious as the conventional method for producing @entity3883 , and internal biliary drainage could be readily available. With the present model, neutrophil overactivity in @entity3883 was effectively alleviated by internal biliary drainage. The result may support the role of preoperative biliary drainage in the prevention of postoperative septic complications.

Neutrophil chemotaxis in bile duct-obstructed XXXX , and effect of internal biliary drainage.

@entity3883 :: ('MESH:D041781', 'Disease') :: ['obstructive jaundice', 'sufficient obstructive jaundice'] @entity2635 :: ('CHEBI:60160', 'Chemical') :: ['polyester'] @entity648 :: ('MESH:D001663', 'Chemical') :: ['bilirubin'] @entity1448 :: ('MESH:D002779', 'Disease') :: ['bile duct obstruction'] @entity35 :: ('10116', 'Species') :: ['rat', 'Rats', 'rats'] @entity493 :: ('CHEBI:16449', 'Chemical') :: ['alanine']
@entity35 :: (10116,Species) :: ['rats']
