PURPOSE: Currently, there are no molecular biomarkers for the early detection of @entity0 . This study focused on identifying surface proteins found on circulating extracellular vesicles (EVs) for detecting @entity0 . EXPERIMENTAL DESIGN: Circulating EVs, isolated from the plasma of 10 @entity1 with @entity0 (stages I and II) and 5 healthy controls, were analyzed using LC-MS/MS. @entity24965 ( @entity24965 ) was selected as a candidate biomarker. Two different ELISAs were used to measure @entity24965 in plasma samples from healthy controls (n = 81), @entity1 with @entity0 (n = 269), @entity0 @entity1 after surgical resection (n = 50), @entity1 with @entity0 (n = 64), and @entity1 with @entity66 (n = 98) in two cohorts. RESULTS: Plasma @entity24965 levels were significantly higher (P < 0.0001) in @entity1 with @entity0 than in all controls and returned to almost normal after @entity5 removal. The diagnostic accuracy of @entity24965 was AUC, 0.961 [95% confidence interval (CI), 0.924-0.983], sensitivity of 94.70%, and specificity of 86.36% in test cohort and 0.968 (0.933-0.988), 92.31%, and 86.62% in validation cohort for @entity0 by one type of ELISA. Furthermore, @entity24965 maintained diagnostic accuracy for @entity1 with @entity0 using the other type of ELISA [0.946 (0.905-0.972), 90.90%, and 77.14% in the test cohort; 0.943 (0.900-0.971), 89.23%, and 80.99% in the validation cohort]. CONCLUSIONS: @entity24965 on circulating EVs is a promising marker to improve identification of @entity1 with @entity0 and distinguish @entity0 from @entity0 and @entity66 . Clin Cancer Res; 1-10.   2015 AACR.

Identification of XXXX on Circulating Extracellular Vesicles as a Novel Biomarker for Early Breast Cancer Detection.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity0 :: ('MESH:D001943', 'Disease') :: ['benign breast tumors', 'breast cancer', 'early-stage breast cancer'] @entity66 :: ('MESH:D004194', 'Disease') :: ['noncancerous diseases'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity24965 :: ('10085', 'Gene') :: ['Developmental endothelial locus-1 protein', 'Del-1']
@entity24965 :: (10085,Gene) :: ['Developmental Endothelial Locus-1']
