BACKGROUND: It has been reported that collagenases ( @entity5780 [ @entity5780 ] and @entity1235 [ @entity1235 ]) are associated with hair cycle, whereas the mechanism of the association is largely unknown. METHODS: The @entity19 were randomly allocated into four groups: saline, and 5, 10, and 15 nM SB-3CT. Immunohistochemical analysis was employed to examine @entity5780 and @entity1235 protein. Real-time polymerase chain reaction and enzyme-linked immunosorbent assay were performed to determine mRNA and protein levels of @entity1611 , @entity7424 , @entity641 , and @entity26637 . Growing hair follicles from anagen phase III-IV were scored based on @entity1710 and @entity1711 staining. Hair regrowth was also evaluated. RESULTS: Results showed that mRNA expressions of enzymes changed with a peak at late anagen and a trough at telogen after depilation. Immunostaining showed that the highest expression of @entity5780 was more than that of @entity1235 , and the highest expression of enzymes changed during anagen. The localizations of @entity5780 changed from dermal papilla, keratinocyte strand, out of root sheath, and basal plate at early anagen, to hair bulb, inner root sheath, and outer root sheath at late anagen. The localization of @entity1235 changed from partial keratinocyte to dermal papilla at early anagen and to outer root sheath at late anagen. @entity1611 , @entity7424 , and @entity641 have been shown to regulate hair growth. We found mRNA and protein expressions of @entity1611 and @entity7424 fluctuated with a peak at anagen and a decrease at catagen to telogen. In contrast, mRNA and protein expressions of @entity641 changed with highest and lowest levels at anagen and telogen, respectively. With selective inhibitor of collagenase IV, SB-3CT, @entity19 showed significant suppressed hair growth and decreased expression of @entity1611 , @entity7424 , and @entity641 . The MMPs agonist also significantly increased expression of @entity1611 , @entity7424 , and @entity641 . Meanwhile, SB-3CT treatment significantly suppressed hair growth. CONCLUSION: All these data suggest that the type IV collagenases, @entity5780 and @entity1235 , play important roles in hair cycle, and this could be mediated by induced expression of @entity1611 , @entity7424 , and @entity641 .

Collagenase IV plays an important role in regulating hair cycle by inducing XXXX , @entity7424 , and @entity641 expression.

@entity19 :: ('10090', 'Species') :: ['mice'] @entity641 :: ('21803(Tax:10090)', 'Gene') :: ['TGF-b'] @entity5780 :: ('17390(Tax:10090)', 'Gene') :: ['MMP-2', 'matrix metalloproteinase 2'] @entity26637 :: ('14433(Tax:10090)', 'Gene') :: ['GAPDH'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity1235 :: ('17395(Tax:10090)', 'Gene') :: ['matrix metalloproteinase 9', 'MMP-9'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hematoxylin'] @entity1611 :: ('22339(Tax:10090)', 'Gene') :: ['VEGF'] @entity7424 :: ('16000(Tax:10090)', 'Gene') :: ['IGF-1']
@entity1611 :: (22339(Tax:10090),Gene) :: ['VEGF']
