BACKGROUND: Anterior cruciate ligament (ACL) tear is considered a risk factor for @entity571 development. The purpose of our study was to investigate the expression levels of the apoptotic enzyme @entity522 , pro-inflammatory cytokines @entity1356 ( @entity1356 ) and @entity650 ( @entity650 ) and degrading enzyme @entity1550 ( @entity1550 ), all indicative of @entity528 and @entity571 development in @entity1 ' chondrocytes after @entity6151 . METHODS: We investigated the correlation between grade of cartilage degradation and time from injury or @entity1 ' age. @entity1356 , @entity650 and @entity1550 mRNA expression levels were investigated in normal (n   =   4) and chondrocytes from @entity1 with @entity6151 (n   =   33) using real-time polymerase chain reaction (PCR). Moreover, @entity1550 and @entity522 protein expression levels were evaluated by western blot analysis. Trend analysis and correlation coefficient were performed to derive the relations between gene expression ( @entity1550 , @entity650 , @entity1356 ) and grading of @entity1844 and between gene expression ( @entity1550 , @entity650 , @entity1356 ) and @entity1 ' age, respectively. RESULTS: Correlations were established between grade of cartilage degradation and time from injury. @entity1550 , @entity650 , @entity1356 and @entity522 expression levels were significantly upregulated in chondrocytes from @entity1347 knee compared to normal. Among the @entity1 with @entity1347 knees, a significant upregulation of @entity1550 was observed in @entity1 with ACL-rupture   >   18  months from the time of injury to arthroscopy compared to @entity1 with @entity130 up to 18  months, whereas @entity650 and @entity1356 expression was higher in chondrocytes from @entity1 with more than 10  months @entity130 compared to those that underwent surgery within the first 10  months after injury.      association was observed between @entity1356 , @entity650 and @entity1550 expression levels and @entity1844 or @entity1 ' age. CONCLUSION: Our results showed that increased levels of apoptotic, inflammatory and catabolic factors in chondrocytes are associated with time from injury and could contribute to cartilage degradation and @entity571 development after @entity6151 .

Molecular changes indicative of @entity528 and @entity571 development in @entity1 with XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity650 :: ('3569', 'Gene') :: ['IL-6', 'interleukin-6'] @entity1844 :: ('MESH:D002357', 'Disease') :: ['cartilage defects'] @entity522 :: ('836', 'Gene') :: ['caspase-3', 'caspase 3'] @entity571 :: ('MESH:D010003', 'Disease') :: ['osteoarthritis'] @entity1356 :: ('3553', 'Gene') :: ['IL-1b', 'interleukin-1b'] @entity1347 :: ('MESH:C537775', 'Disease') :: ['ACL-deficient'] @entity1550 :: ('4322', 'Gene') :: ['MMP-13', 'matrix metalloproteinase 13', 'MMP13'] @entity6151 :: ('MESH:D057971', 'Disease') :: ['ACL rupture'] @entity528 :: ('MESH:D009410', 'Disease') :: ['cartilage degeneration'] @entity130 :: ('MESH:D014947', 'Disease') :: ['ACL injury', 'ACL-injury']
@entity130 :: (MESH:D014947,Disease) :: ['anterior cruciate ligament injury']
