UNASSIGNED: Elevated expression levels of @entity4043 ( @entity4043 ) promote @entity5 development and progression. MAP kinase interacting kinases (MNKs) modulate the function of @entity4043 through the phosphorylation that is necessary for oncogenic transformation. Therefore, pharmacologic MNK inhibitors may provide a nontoxic and effective anticancer strategy. MNK1b is a truncated isoform of MNK1a that is active in the absence of stimuli. Using in vitro selection, high-affinity DNA aptamers to MNK1b were selected from a library of ssDNA. Selection was monitored using the enzyme-linked oligonucleotide assay (ELONA), and the selected aptamer population was cloned and sequenced. Four groups of aptamers were identified, and the affinities of one representative for rMNK1b were determined using ELONA and quantitative polymerase chain reaction. Two aptamers, named apMNK2F and apMNK3R, had a lower Kd in the nmol/l range. The secondary structure of the selected aptamers was predicted using mFold, and the QGRS Mapper indicated the presence of potential G-quadruplex structures in both aptamers. The selected aptamers were highly specific against @entity23815 , showing higher affinity to MNK1b than to MNK1a. Interestingly, both aptamers were able to produce significant translation inhibition and prevent @entity5 cell proliferation and migration and colony formation in @entity0 cells. These results indicate that @entity23815 aptamers have an attractive therapeutic potential.

Characterization of MNK1b DNA Aptamers That Inhibit Proliferation in MDA-MB231 XXXX Cells.

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity4043 :: ('1977', 'Gene') :: ['eIF4E', 'eukaryotic initiation factor 4E'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity23815 :: ('8569', 'Gene') :: ['MNK1']
@entity0 :: (MESH:D001943,Disease) :: ['Breast Cancer']
