UNASSIGNED: @entity14301 complex spotting (LP), the result of an incompletely dominant mutation in TRPM1, produces a collection of unique depigmentation patterns in the @entity1641 . Although the LP mutation allows for expression of the various patterns, other loci are responsible for modification of the extent of white. Pedigree analysis of families segregating for high levels of patterning indicated a single dominant gene, named Pattern-1 (PATN1), as a major modifier of LP. Linkage analysis in two half-sibling families segregating for PATN1 identified a 15-Mb region on ECA3p that warranted further investigation. Whole transcriptome sequencing of skin samples from @entity1641 with and without the PATN1 allele was performed to identify genic SNPs for fine mapping. Two Sequenom assays were utilized to genotype 192 individuals from five LP-carrying breeds. The initial panel highlighted a 1.6-Mb region without a clear candidate gene. In the second round of fine mapping, SNP ECA3:23  658  @entity68791 in the 3'-UTR of @entity68792 ( @entity68792 ) reached a significance level of P  =  1.063  *  10(-39) . Sequencing of @entity68792 did not identify any coding polymorphisms specific to PATN1 @entity1641 . Genotyping of the @entity68792 3'-UTR SNP in 54 additional LP animals and 327 @entity1641 from nine breeds not segregating for LP further supported the association (P  =  4.17  *  10(-115) ). This variant is a strong candidate for PATN1 and may be particularly useful for LP breeders to select for high levels of white patterning.

Variant in the XXXX gene associated with PATN1, a modifier of @entity14301 complex spotting.

@entity68792 :: ('100055114(Tax:9796)', 'Gene') :: ['RING finger and WD repeat domain 3', 'RFWD3'] @entity1641 :: ('9796', 'Species') :: ['horse', 'horses'] @entity68791 :: ('c|SUB|T|447|G', 'DNAMutation') :: ['447T>G'] @entity14301 :: ('9691', 'Species') :: ['Leopard']
@entity68792 :: (100055114(Tax:9796),Gene) :: ['RFWD3']
