BACKGROUND: Rodent lungs undergo full histologic recovery within 1 week following unilateral lung contusion (LC). However, when LC is followed by @entity548 ( @entity548 ), healing is impaired. We hypothesize that the intravenous administration of mesenchymal stem cells (MSCs) in animals undergoing combined LC followed by @entity548 (LCHS) will improve wound healing. METHODS: Male @entity35 (n = 5-6 per group) were subjected to LCHS with or without the injection of a single intravenous dose of 5 * 10 MSCs following return of shed blood after @entity548 . @entity35 were sacrificed 7 days following injury. Flow cytometry was used to determine the T-regulatory cell (Treg) population in peripheral blood. Lung histology was graded using a well-established @entity651 ( @entity651 ). Components of the @entity651 include average inflammatory cells per high-power field over 30 fields, @entity4 , @entity3126 , and alveolar integrity, with total scores ranging from 0 to 11. Data were analyzed by analysis of variance followed by Tukey's multiple comparison test, expressed as mean (SD). p < 0.05 was considered significant. RESULTS: Seven days following isolated LC, animals demonstrated lung healing with an @entity651 unchanged from naive. The addition of @entity548 resulted in a persistently elevated @entity651 score, whereas the addition of MSCs to LCHS decreased the @entity651 score back to naive levels. The change in @entity651 was driven by a significant decrease in @entity4 scores. In @entity35 undergoing LC alone, 10.5% (3.3%) of CD4 cells were Tregs. The addition of @entity548 caused no significant change in Treg population (9.3% [0.7%]), whereas LCHS + MSC significantly increased the population to 18.2% (6.8%) in peripheral blood (p < 0.05 vs. LCHS). CONCLUSION: Impaired wound healing following @entity130 and @entity548 is improved by a single dose of MSCs given immediately after injury. This enhanced healing is associated with an increase in the Treg population and a significant @entity4 score as compared with animals undergoing LCHS. Further study into the role of Tregs in MSC-mediated wound healing is warranted.

Mesenchymal stem cells increase T-regulatory cells and improve healing following XXXX and @entity548 .

@entity3126 :: ('MESH:D011654', 'Disease') :: ['pulmonary edema'] @entity548 :: ('MESH:D006470', 'Disease') :: ['hemorrhagic shock', 'HS'] @entity4 :: ('MESH:D004487', 'Disease') :: ['edema', 'decrease in lung edema', 'interstitial edema'] @entity651 :: ('MESH:D055370', 'Disease') :: ['lung injury score', 'LIS'] @entity35 :: ('10116', 'Species') :: ['Sprague-Dawley rats', 'Rats', 'rats'] @entity130 :: ('MESH:D014947', 'Disease') :: ['trauma']
@entity130 :: (MESH:D014947,Disease) :: ['trauma']
