UNASSIGNED: Arrest of replication fork progression is one of the most common causes for increasing the genomic instability. In bacteria, PriA, a conserved DEXH-type helicase, plays a major role in recognition of the stalled forks and restart of DNA replication. We took advantage of PriA's ability to specifically recognize stalled replication forks to determine the genomic loci where replication forks are prone to stall on the @entity526 genome. We found that PriA binds around oriC upon @entity6244 starvation which reduces the @entity4334 supply and causes replication fork stalling. PriA binding quickly disappeared upon readdition of @entity6244 . Furthermore, @entity1603 was incorporated at around oriC upon release from @entity6244 starvation. Our results indicate that reduced supply of DNA replication precursors causes replication fork stalling preferentially in the 600 kb segment centered at oriC. This suggests that replication of the vicinity of oriC requires higher level of @entity4334 precursors. The results also point to a possibility of slow fork movement and/or the presence of multiple fork arrest signals within this segment. Indeed, we have identified rather strong fork stall/pausing signals symmetrically located at    50 kb away from oriC. We speculate that replication pausing and fork-slow-down shortly after initiation may represent a novel checkpoint that ensures the presence of sufficient @entity4334 supply prior to commitment to duplication of the entire genome.

Fork restart protein, PriA, binds around oriC after depletion of XXXX precursors: replication fork arrest near the replication origin.

@entity1603 :: ('MESH:D001973', 'Chemical') :: ['BrdU'] @entity4334 :: ('D009711', 'Chemical') :: ['nucleotide'] @entity6244 :: ('MESH:D013941', 'Chemical') :: ['thymine'] @entity526 :: ('562', 'Species') :: ['Escherichia coli']
@entity4334 :: (D009711,Chemical) :: ['nucleotide']
