Macrophages play a central role during the pathogenesis of @entity32 . In normal intestinal mucosa surface expression of typical macrophage markers such as @entity5750 , @entity4071 , @entity9536 or T-cell co-stimulatory molecules such as @entity7938 or @entity2937 is low indicating anergy and low pro-inflammatory activity of these cells. During @entity29 ( @entity29 ) the mucosa is invaded by a population of macrophages displaying these markers, secreting higher cytokine levels and representing an activated cell population. @entity1116 (+) cells (macrophages) were isolated from normal and @entity30 mucosa and mRNA was isolated by polyT magnetic beads. A subtractive screening was performed subtracting mRNA from normal macrophages from those of @entity30 macrophages. Oxidative burst activity was determined by flow cytometry. Seventy clones were obtained by the subtractive mRNA screening. Sequencing showed > 99% homology to mRNA of @entity2842 ( @entity2842 ) for three clones. Five clones obtained by subtraction revealed > 99% homology to mRNA of @entity13508 (subunit gp91). Differential expression of the @entity13508 subunit gp91 and the cytosolic @entity1110 oxidase subunit @entity1116 was confirmed by RT-PCR and 'virtual' Northern blots. The fluorescence ratio of stimulated versus unstimulated cells was 0.9 +/- 0.16 in control macrophages indicating a lack of oxidative burst activity. In @entity30 this ratio was significantly increased to 1.80 +/- 0.8 (P = 0.004) confirming the molecular data. In conclusion @entity1110 oxidase mRNA is down-regulated or absent in macrophages from normal mucosa correlating with a lack of oxidative burst activity. In @entity29 macrophage-oxidative burst activity is increased and @entity1110 oxidase mRNA induced. Inhibition of @entity1110 oxidase could be a new therapeutical target in @entity29 and reduce mucosal tissue damage in active @entity29 .

Subtractive screening reveals up-regulation of @entity1110 oxidase expression in XXXX intestinal macrophages.

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity2937 :: ('942', 'Gene') :: ['CD86'] @entity5750 :: ('929', 'Gene') :: ['CD14'] @entity7938 :: ('941', 'Gene') :: ['CD80'] @entity29 :: ('MESH:D015212', 'Disease') :: ['inflammatory bowel disease', 'IBD'] @entity1110 :: ('MESH:D009249', 'Chemical') :: ['NADPH'] @entity2842 :: ('6347', 'Gene') :: ['MCP-1', 'monocyte chemoattractant protein-1'] @entity4071 :: ('2214', 'Gene') :: ['CD16'] @entity13508 :: ('4519', 'Gene') :: ['cytochrome b'] @entity9536 :: ('3684', 'Gene') :: ['CD11b'] @entity30 :: ('MESH:D003424', 'Disease') :: ["Crohn's disease"] @entity1116 :: ('945', 'Gene') :: ['p67', 'CD33']
@entity30 :: (MESH:D003424,Disease) :: ['Crohn's disease']
