UNASSIGNED: @entity4823  ( @entity4824 ) has been widely used to treat @entity571  ( @entity571 ) in @entity1 . However, the effects of @entity4824 on genes related to cartilage metabolism are still unknown. In the present study, to elucidate the chondroprotective action of @entity4824 on @entity571 , we examined the effects of @entity4824  (0.1-10  mM) on the expression of the sirtuin  (SIRT) genes as well as type  II collagen and matrix metalloproteinases  (MMPs) using a @entity1 chondrocyte cell line  @entity1 . @entity1  cells were incubated in the absence or presence of @entity4824 . RT-PCR analyses revealed that @entity4824 markedly increased the mRNA expression of type  II collagen  ( @entity794 ). By contrast, the levels of @entity3334 and  @entity1495 mRNA were only slightly increased by @entity4824 . Furthermore, western blot analyses revealed that @entity4824 significantly increased the protein level of @entity794 . Importantly, @entity4824 enhanced the mRNA expression and protein level of @entity4093 , an upstream-regulating gene of @entity794 . Moreover, a @entity4093 inhibitor suppressed @entity4824 -induced @entity794  gene expression. Together these observations suggest that @entity4824 enhances the mRNA expression and protein level of @entity4093 and its downstream gene @entity794 in chondrocytes, thereby possibly exhibiting chondroprotective action on @entity571 .

Effect of @entity4823 on expression of type II collagen, matrix metalloproteinase and sirtuin genes in a XXXX chondrocyte cell line.

@entity1 :: ('9606', 'Species') :: ['humans', 'human', 'SW  1353'] @entity3334 :: ('4312', 'Gene') :: ['MMP-1'] @entity571 :: ('MESH:D010003', 'Disease') :: ['OA', 'osteoarthritis'] @entity4093 :: ('23411', 'Gene') :: ['SIRT1'] @entity4823 :: ('MESH:D005944', 'Chemical') :: ['Glucosamine'] @entity794 :: ('1280', 'Gene') :: ['COL2A1'] @entity4824 :: ('CHEBI:5417', 'Chemical') :: ['GlcN'] @entity1495 :: ('4318', 'Gene') :: ['MMP-9']
@entity1 :: (9606,Species) :: ['human']
