UNASSIGNED: This study was to investigate the effects of Rosa laevigata Michx. (RLM) extract on reactive @entity26 species (ROS) production and mitochondrial membrane potential (MMP) in lens epithelial cells (LECs) cultured under high @entity413 . SRA01/04 cell models of @entity6 were established by high @entity413 culture, and these cells were administrated with RLM extract. Levels of ROS and MMP in SRA01/04 cells were detected with fluorescent probes. @entity13975 expression level, @entity584 phosphorylation, and @entity13791 translocation were detected by Western blot. RLM treatment could exert protective effects on SRA01/04 cells under high @entity413 condition, decreasing the ROS production and elevating the MMP in these cells. Western blot analysis indicated that the expression level of @entity13975 was significantly elevated in SRA01/04 cells after the RLM treatment. Moreover, when @entity13975 was interfered with siRNA, the effects of RLM on the levels of ROS and MMP were diminished, indicating that @entity13975 induction was necessary for the function of RLM. Furthermore, @entity13975 inducing effects of RLM were mediated by the PI3K/ @entity584 and @entity13791 /ARE pathways. The effects would be abolished when either pathway was inhibited by pharmacological manipulation or siRNA silencing. RLM inhibits ROS production and elevates MMP, through the induction of @entity13975 expression, in LECs under high @entity413 condition. The protective effects of RLM are mediated by the PI3K/ @entity584 and @entity13791 /ARE pathways. Our findings provide theoretic basis and experimental evidence for the application of RLM in the treatment of @entity6 .

Effects of Rosa laevigata Michx. extract on reactive XXXX species production and mitochondrial membrane potential in lens epithelial cells cultured under high @entity413 .

@entity6 :: ('MESH:D003920', 'Disease') :: ['diabetic cataract'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity584 :: ('207', 'Gene') :: ['Akt'] @entity13791 :: ('4780', 'Gene') :: ['Nrf2'] @entity13975 :: ('3162', 'Gene') :: ['HO-1'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity26 :: (MESH:D010100,Chemical) :: ['oxygen']
