@entity8415 such as @entity2469 ( @entity19594 ) are cytotoxic to suspensions of isolated hepatocytes. The exact mechanism of @entity137 is unknown but may involve peroxidation of cellular lipids, alkylation of cellular macromolecules, or alterations in cellular @entity460 homeostasis. These studies were designed to examine lipid peroxidation as a mechanism of organic @entity273 -induced cell death. Hepatocytes isolated from @entity19 were more susceptible to the cytotoxic effects of @entity19594 than were @entity35 hepatocytes. @entity19594 -induced cell death was preceded by @entity1234 formation which was also greater in @entity19 than @entity35 hepatocytes. Species differences in lipid peroxidation were due to intrinsic properties of hepatocyte membranes as lipids isolated from @entity19 liver and peroxidized with @entity68 / @entity9050 formed approximately eightfold more @entity1234 than lipids isolated from @entity35 liver. Initiation of lipid peroxidation in @entity19 and @entity35 hepatocytes with @entity68 / @entity9050 caused the formation of @entity1234 equal to that seen with @entity19594 and a slight depletion of cellular @entity2197 . As with @entity19594 , @entity1234 formation induced by @entity68 / @entity9050 was greater in @entity19 than in @entity35 hepatocytes. However, @entity68 / @entity9050 had no effect on hepatocyte viability or morphology from either species. Furthermore, @entity19594 -induced @entity1234 and @entity17487 formation in isolated @entity35 hepatocytes were completely blocked by @entity4489 whereas cell @entity137 was altered only slightly. Therefore, these data do not support a role for lipid peroxidation in the acute @entity137 of @entity19594 to suspensions of isolated @entity35 hepatocytes.

Organic XXXX -induced lipid peroxidation and cell death in isolated hepatocytes.

@entity8415 :: ('CHEBI:35924', 'Chemical') :: ['Organic hydroperoxides'] @entity4489 :: ('MESH:D011398', 'Chemical') :: ['promethazine'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity', 'cytotoxicity'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity17487 :: ('MESH:D004980', 'Chemical') :: ['ethane'] @entity19594 :: ('CHEBI:64090', 'Chemical') :: ['TBHP'] @entity2197 :: ('CHEBI:16856', 'Chemical') :: ['GSH'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['hydroperoxide'] @entity1234 :: ('MESH:D008315', 'Chemical') :: ['malondialdehyde'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity2469 :: ('MESH:D020122', 'Chemical') :: ['tert-butyl hydroperoxide'] @entity9050 :: ('CHEBI:38290', 'Chemical') :: ['ascorbate'] @entity68 :: ('MESH:D007501', 'Chemical') :: ['iron']
@entity273 :: (MESH:D006861,Chemical) :: ['hydroperoxide']
