@entity164 2 @entity6 results from the failure of beta-cells to adequately compensate for insulin resistance. Although the reduction of beta-cell mass is because of increased cell death and/or inadequate replication or neogenesis, the mechanism underlying beta-cell mass reduction is not fully understood. Here, we clarify the role of insulin signaling pathway in the beta-cell apoptosis using insulin resistant model @entity19 . Wild-type @entity19 and those carrying a mutation in the @entity3239 ( @entity21445 ) were fed either regular chow or a high-fat diet for 6 weeks and subsequently investigated for beta-cell apoptosis, @entity1642 , and oxidative stress. Insulin tolerance tests revealed that @entity21445 @entity19 fed a high-fat diet (mIRHF) had higher insulin resistance. Beta-cell apoptosis was increased 2-fold in the wild-type @entity19 fed a high-fat diet (wHF) compared with control @entity19 , whereas beta-cell apoptosis in mIRHF @entity19 did not increase compared with that in @entity21445 @entity19 . The expression of @entity1642 markers in isolated islets did not differ between the groups. Staining of @entity7599 and @entity11838 in islets of wHF @entity19 significantly increased, but the staining in mIRHF @entity19 was not different from that in control group. Gene expression of the antioxidant enzyme @entity183 was significantly higher in mIRHF @entity19 than those in the other 3 groups. A mutation in the @entity3239 attenuated the oxidative stress and apoptosis in beta-cells even though high caloric nutrient was loaded. Our results suggest that reduced insulin signaling protects beta-cells thorough decline of oxidative stress.

Mutation in XXXX Attenuates Oxidative Stress and Apoptosis in Pancreatic Beta-Cells Induced by Nutrition Excess: Reduced Insulin Signaling and ROS.

@entity7599 :: ('MESH:C040791', 'Chemical') :: ["8-hydroxy-2'-deoxyguanosine"] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetes'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity3239 :: ('16337(Tax:10090)', 'Gene') :: ['insulin receptor'] @entity21445 :: ('751557(Tax:10090)', 'Gene') :: ['mIR'] @entity164 :: ('MESH:D017827', 'Disease') :: ['Type'] @entity183 :: ('20656(Tax:10090)', 'Gene') :: ['MnSOD'] @entity1642 :: ('MESH:D015775', 'Disease') :: ['endoplasmic reticulum stress'] @entity11838 :: ('MESH:C027576', 'Chemical') :: ['4-hydroxy-2-nonenal']
@entity3239 :: (16337(Tax:10090),Gene) :: ['Insulin Receptor']
