UNASSIGNED: @entity571 ( @entity571 ) is a @entity102 affecting the cartilage of over 15% of Canadians. Synovial fluid mesenchymal progenitor cells (sfMPCs) are present in joints and are thought to contribute to healing. @entity571 sfMPCs have a greater proliferative ability but decreased chondrogenic potential. However, little is known about the factors influencing/regulating the differences between normal and @entity571 sfMPCs. Recently, our lab has shown that sfMPC chondrogenic differentiation in vitro is favorably biased toward a similar osmotic environment as they experience in vivo. The current study now examines the expression and functionality of a variety of ion channels in sfMPCs derived from normal individuals and early @entity571 @entity1 . Results indicated that there is differential ion channel regulation at the functional level and expression level in early @entity571 sfMPCs. All ion channels were upregulated in early @entity571 compared to normal sfMPCs with the exception of @entity4030 at the mRNA level. At the protein level, @entity23128 was over expressed in early @entity571 sfMPCs, while @entity19378 and @entity4030 were unchanged between normal and early @entity571 sfMPCs. At the functional level, the inward rectifying @entity972 channel was under expressed in early @entity571 sfMPCs, however the membrane potential was unchanged between normal and early @entity571 sfMPCs. In the synovial environment itself, a number of differences in ion concentration between normal and early @entity571 synovial fluid were observed. These findings suggest that normal and @entity571 progenitor cells demonstrate functional differences in how they interact with the synovial ion environment.

Ion channel expression and function in normal and osteoarthritic XXXX synovial fluid progenitor cells.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity571 :: ('MESH:D010003', 'Disease') :: ['OA', 'Osteoarthritis'] @entity23128 :: ('59341', 'Gene') :: ['TRPV4'] @entity4030 :: ('3778', 'Gene') :: ['KCNMA1'] @entity102 :: ('MESH:D002908', 'Disease') :: ['chronic disease'] @entity19378 :: ('3768', 'Gene') :: ['KCNJ12'] @entity972 :: ('MESH:D011188', 'Chemical') :: ['potassium']
@entity1 :: (9606,Species) :: ['human']
