The expression of Fas ligand ( @entity9699 ) on @entity5 cells ( @entity5 @entity9699 ) has been implicated in their evasion of immune surveillance. In this study, we investigated the cellular mechanism for @entity9699 -associated immune escape using @entity320 @entity19 -derived cells as a model. Transfectants carrying @entity9699 -specific ribozymes expressed low levels of @entity9699 ( @entity9699 (low) @entity5 cells) as compared with those carrying enhanced green fluorescent protein-N1 plasmids ( @entity9699 (high) @entity5 cells). When injected s.c. into C57BL/6 @entity19 , @entity9699 (low) @entity5 cells grew more slowly than did @entity9699 (high) @entity320 cells. @entity9699 (high) @entity5 cells showed more intensive neutrophilic infiltration accompanied by multiple @entity39 areas than did @entity9699 (low) @entity5 cells. The average size of @entity9699 (low) @entity5 , but not of @entity9699 (high) @entity5 , was significantly enhanced in @entity19 depleted of neutrophils. Consistently, a local injection of LPS to recruit/activate neutrophils significantly delayed @entity5 formation by @entity9699 (low) @entity5 cells, and slightly retarded that of @entity9699 (high) @entity5 cells in both C57BL/6 and nonobese @entity6 / @entity915 @entity19 . Neutrophils killed @entity9699 (low) @entity320 cells more effectively than @entity9699 (high) @entity320 cells in vitro. The resistance of @entity9699 (high) @entity320 cells to being killed by neutrophils was correlated with impaired neutrophil activation, as demonstrated by reductions in @entity1235 secretion, reactive @entity26 species production, and the surface expression of @entity3804 and the transcription of @entity9699 . Local transfer of casein-enriched or @entity4515 -treated neutrophils delayed @entity5 formation by @entity320 cells. Taken together, inactivation of neutrophils by @entity5 @entity9699 is an important mechanism by which @entity5 cells escape immune attack.

Fas ligand on XXXX cells mediates inactivation of neutrophils.

@entity6 :: ('MESH:D003920', 'Disease') :: ['diabetic'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrotizing'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity19 :: ('10090', 'Species') :: ['mice', 'B16F10'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity4515 :: ('CHEBI:60755', 'Chemical') :: ['PMA'] @entity915 :: ('MESH:D053632', 'Disease') :: ['SCID'] @entity1235 :: ('17395(Tax:10090)', 'Gene') :: ['gelatinase B'] @entity9699 :: ('14103(Tax:10090)', 'Gene') :: ['FasL'] @entity3804 :: ('16409(Tax:10090)', 'Gene') :: ['CD11b'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma']
@entity5 :: (MESH:D009369,Disease) :: ['tumor']
