@entity5445 ( @entity5445 ) is released from neuroendocrine cells in the intestine in the postprandial state and augments @entity413 -stimulated @entity2690 secretion from pancreatic beta cells. To develop non-beta cells that exhibit physiologically regulated @entity2690 secretion, we coexpressed the @entity8142 and @entity1 @entity2690 in primary @entity35 pituitary cells using adenovirus-mediated gene transfer. The transduced cells were analyzed in a perifusion system and after transplantation into @entity19 . Normal pituitary cells do not express the @entity8142 as shown by the absence of @entity8142 mRNA and the inability of @entity5445 to stimulate pituitary hormone secretion. Following transduction with an adenovirus carrying the @entity8142 cDNA, the pituitary cells expressed functional @entity5445 receptors as reflected by the ability of @entity5445 to stimulate secretion of pituitary hormones. When both the @entity8142 and @entity1 @entity2690 were introduced, @entity5445 stimulated cosecretion of @entity1 @entity2690 and endogenous pituitary hormones. @entity5445 was similar in potency to the hypothalamic-releasing hormones and stimulated hormone secretion in a dose-dependent fashion. In contrast to pancreatic beta cells, the hormone-releasing effect of @entity5445 on transduced pituitary cells was not dependent on the concentration of extracellular @entity413 . After transplantation of pituitary cells coexpressing @entity1 @entity2690 and @entity8142 into @entity19 , enteral @entity413 stimulated @entity2690 secretion. These results demonstrate a new approach to engineer physiologically regulated @entity2690 secretion by non-beta cells.

Engineering physiologically regulated @entity2690 secretion in non-beta cells by expressing XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity2690 :: ('3630', 'Gene') :: ['insulin'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity8142 :: ('2740', 'Gene') :: ['GLP-1 receptor'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity5445 :: ('2641', 'Gene') :: ['Glucagon-like peptide 1', 'GLP-1']
@entity8142 :: (2740,Gene) :: ['glucagon-like peptide 1 receptor']
