OBJECTIVE: To compare the regenerative capacity of diseased bladder in a large animal model of @entity1494 with regeneration in healthy bladder using a highly porous collagen scaffold. MATERIALS AND METHODS: Highly porous @entity291 type I collagen scaffolds with a diameter of 32 mm were prepared. In 12 fetal @entity742 a @entity1494 was surgically created at 79 days' gestation. @entity742 were born at full term (140 days' gestation). After 1 week the @entity1494 was reconstructed and augmented with a collagen scaffold (group 1). In nine normal newborn @entity742 the bladder was augmented with a collagen scaffold 1 week after birth (group 2). Functional (video-urodynamics) and histological evaluation was performed at 1 and 6 months after surgery. RESULTS: The survival rate was 58% in group 1 and 100% in group 2. Cystograms were normal in all @entity742 , besides low-grade reflux in both groups. Urodynamics showed comparable capacity between both groups and a trend to lower compliance in group 1. Histological evaluation at 1 month revealed a non-confluent urothelial layer, an immature submucosa, and initial ingrowth of smooth muscle cells. At 6 months both groups showed normal urothelial lining, standard extracellular matrix development, and smooth muscle cell ingrowth. CONCLUSIONS: Bladder tissue regeneration with a collagen scaffold in a diseased bladder model and in healthy bladder resulted in comparable functional and histological outcome, with a good quality of regenerated tissue involving all tissue layers. Improvements may still be needed for larger augmentations or more severely diseased bladders.

Tissue engineering of diseased bladder using a collagen scaffold in a XXXX model.

@entity291 :: ('9913', 'Species') :: ['bovine'] @entity742 :: ('9940', 'Species') :: ['Lambs', 'sheep', 'lambs'] @entity1494 :: ('MESH:D001745', 'Disease') :: ['bladder exstrophy', 'bladder lesion']
@entity1494 :: (MESH:D001745,Disease) :: ['bladder exstrophy']
