UNASSIGNED: Tissue decellularization strategies have enabled engineering of scaffolds that preserve native extracellular matrix (ECM) structure and composition. In this study, we developed decellularized retina (decell-retina) thin films. We hypothesized that these films, mimicking the retina niche, would promote @entity1 retinal progenitor cell ( @entity69150 ) attachment, proliferation and differentiation. Retinas isolated from @entity291 eyes were decellularized using 1% w/v @entity3208 ( @entity214 ) and pepsin digested. The resulting decell-retina was biochemically assayed for composition and cast dried to develop thin films. Attachment, viability, morphology, proliferation and gene expression of @entity69150 cultured on the films were studied in vitro. Biochemical analyses of decell-retina compared to native retina indicated the bulk of DNA (94%) was removed, while the majority of sulfated GAGs (55%), collagen (83%), hyaluronic acid (87%), and key growth factors were retained. The decell-retina films supported @entity69150 attachment and growth, with cell number increasing 1.5-fold over a week. RT-PCR analysis revealed @entity69150 expression of @entity8695 , rod outer membrane, neural retina-specific @entity368 zipper neural and cone-rod homeobox gene on decell-retina films, indicating photoreceptor development. In conclusion, novel decell-retina films show promise as potential substrates for culture and/or transplantation of retinal progenitor cells to treat @entity989 . STATEMENT OF SIGNIFICANCE: In this study, we report the development of a novel biomaterial, based on decellularized retina (decell-retina) that mimics the retina niche and promotes @entity1 retinal progenitor cell ( @entity69150 ) attachment, proliferation and differentiation. We estimated, for the first time, the amounts of collagen I, GAGs and HA present in native retina, as well as the decell-retina. We demonstrated that retinas can be decellularized using ionic detergents and can be processed into mechanically stable thin films, which can act as substrates for culturing hRPCs. @entity8695 , @entity46747 , @entity43960 and @entity13464 gene expression on the decell-retina films indicated photoreceptor development from RPCs. These results support the potential of decell-retina as a cell delivery platform to treat and manage @entity989 like @entity13785 .

Decellularized retinal matrix: Natural platforms for XXXX retinal progenitor cell culture.

@entity1 :: ('9606', 'Species') :: ['human'] @entity46747 :: ('6094', 'Gene') :: ['ROM1'] @entity3208 :: ('MESH:D012967', 'Chemical') :: ['sodium dodecyl sulfate'] @entity368 :: ('CHEBI:25017', 'Chemical') :: ['leucine'] @entity8695 :: ('6010', 'Gene') :: ['Rhodopsin', 'rhodopsin'] @entity69150 :: ('8634', 'Gene') :: ['hRPC'] @entity43960 :: ('4901', 'Gene') :: ['NRL'] @entity13464 :: ('1406', 'Gene') :: ['CRX'] @entity13785 :: ('MESH:D006009', 'Disease') :: ['AMD'] @entity989 :: ('MESH:D012164', 'Disease') :: ['retinal degenerative disease', 'retinal degenerative disorders'] @entity291 :: ('9913', 'Species') :: ['bovine'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS']
@entity1 :: (9606,Species) :: ['human']
