@entity34 ( @entity34 ) is an evolving @entity5 treatment with promising results in treating @entity5 by photoactivation of a photosensitizer with a specific wavelength. The second generation photosensitizer @entity37 (NPe6) was reported to have significant efficacy in killing @entity5 cells in vitro and in vivo. Though topical application might yield a higher local concentration and less systemic side effect, no data concerning topical absorption of NPe6 is available even though the drug has already been used in clinical trial for several years. To evaluate the possibility of topical delivery of NPe6 via an animal model, escalated concentrations of NPe6 were applied to BALB/c @entity19 skin for a different time periods after barrier disruption with tape stripping. Since NPe6 fluorescence intensity and drug concentration in tissue was well correlated, we evaluated drug penetration depth with frozen sections of treated and non-treated skin under a fluorescence microscope. An on-line fluorescence imaging system was used to monitor the NPe6 fluorescence kinetics in the skin. The fluorescence microscope confirmed successful topical delivery of NPe6 in @entity19 skin with or even without barrier disruption. Orange to red NPe6 fluorescence appeared at the epidermis, dermis, and even the muscular layer when using 10 mg/ml NPe6 application. The fluorescence intensity peaked at 1 h and revealed a dose-dependent response pattern. NPe6 treated versus non-treated skin showed a statistically significant difference by Student's t-test (P<0.05). The results described here suggest that topical delivery of NPe6 is possible. It showed fast and deep penetration into @entity19 skin. This implies that NPe6 might be useful as a topical photosensitizer for @entity34 in treating @entity1489 .

Pilot study of topical delivery of XXXX (NPe6): implication of topical NPe6-photodynamic therapy.

@entity5 :: ('MESH:D009369', 'Disease') :: ['malignant tumors', 'cancer'] @entity1489 :: ('MESH:D012878', 'Disease') :: ['skin cancers'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity37 :: ('MESH:C053434', 'Chemical') :: ['mono-L-aspartyl chlorin e6'] @entity34 :: ('MESH:D016609', 'Disease') :: ['Photodynamic therapy', 'PDT']
@entity37 :: (MESH:C053434,Chemical) :: ['mono-L-aspartyl chlorin e6']
