UNASSIGNED: It is still challenging to identify causal genes governing @entity28 . @entity68328 , a quantitative trait locus (QTL) for resistance to @entity28 , was previously discovered from wild @entity17415 @entity19 and was fine-mapped to a 2.1-Mb genomic region of @entity19 chromosome 2, where no known gene with an effect on @entity1386 ( @entity1386 ) has been reported. The aim of this study was to identify a strong candidate gene for @entity68328 by an integration approach of transcriptome analysis (RNA-sequencing followed by real-time PCR analysis) and the causal inference test (CIT), a statistical method to infer causal relationships between diplotypes, gene expression and trait values. Body weight, body composition and biochemical traits were measured in F2 @entity19 obtained from an intercross between the C57BL/6JJcl strain and a congenic strain carrying @entity68328 on the C57BL/6JJcl background. The F2 @entity19 showed significant diplotype differences in 12 traits including body weight, @entity1386 weight and serum @entity165 / @entity412 levels. The transcriptome analysis revealed that @entity13384 , @entity69369 , @entity69370 and Gca genes were differentially expressed in the liver and that @entity69370 , @entity68243 and @entity68329 were differentially expressed in @entity1386 . However, CITs indicated statistical evidence that only the liver @entity13384 gene mediated between genotype and @entity1386 . @entity13384 expression was negatively associated with @entity1386 weight. The results suggested that @entity13384 is a putative quantitative trait gene for the @entity28 -resistant @entity68328 QTL discovered from the wild M. @entity17415 @entity19 . The finding provides a novel insight into a better understanding of the genetic basis for prevention of @entity28 .

Identification of a Putative Quantitative Trait Gene for Resistance to Obesity in XXXX Using Transcriptome Analysis and Causal Inference Tests.

@entity68328 :: ('100035194(Tax:10090)', 'Gene') :: ['Pbwg1.5'] @entity68329 :: ('50915(Tax:10090)', 'Gene') :: ['Grb14'] @entity69369 :: ('18779(Tax:10090)', 'Gene') :: ['Pla2r1'] @entity28 :: ('MESH:D009765', 'Disease') :: ['obesity'] @entity69370 :: ('14089(Tax:10090)', 'Gene') :: ['Fap'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity17415 :: ('10091', 'Species') :: ['m. castaneus', 'Mus musculus castaneus'] @entity13384 :: ('17076(Tax:10090)', 'Gene') :: ['Ly75'] @entity412 :: ('CHEBI:17855', 'Chemical') :: ['triglyceride'] @entity68243 :: ('71586(Tax:10090)', 'Gene') :: ['Ifih1'] @entity1386 :: ('MESH:D018205', 'Disease') :: ['WAT', 'white adipose tissue'] @entity165 :: ('MESH:D002784', 'Chemical') :: ['cholesterol']
@entity19 :: (10090,Species) :: ['Mice']
