BACKGROUND/AIMS: Topical @entity1138 ( @entity1139 ) therapy has been used for treatment of ocular surface @entity5 (OSSN) since 1994. Relatively few studies have reported the cellular changes in ocular surface following @entity1139 . METHODS: Impression cytology was studied in four @entity1 with ocular surface @entity5 , either primary or recurrence after previous excisional biopsy. The authors studied samples obtained using Millipore filters at intervals between 4 and 17 weeks after commencement of @entity1139 , and compared them with pretreatment cytology. RESULTS: @entity1139 induced changes of cytomegaly, cytoplasmic vacuolation, nucleomegaly with nuclear wrinkling, and binucleation or multinucleation were seen in some cells in all samples. However, nuclear/cytoplasmic (N/C) ratio in these enlarged cells was normal. These changes mimicked those seen following radiation therapy in uterine cervix. Changes of increased nuclear and cell size with increased N/C ratio were seen in some @entity6349 cells. The predominant form of cell death was apoptosis with fewer cells showing @entity39 . CONCLUSIONS: @entity1139 appears to produce cell death in OSSN by apoptosis and @entity39 . Cellular changes related to @entity1139 mimic those caused by radiation-cytomegaly, nucleomegaly, and vacuolation. @entity1139 related changes may persist in ocular surface epithelium for at least 8 months following @entity1139 therapy.

Impression cytology following @entity1138 therapy for ocular surface XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis'] @entity5 :: ('MESH:D009369', 'Disease') :: ['squamous neoplasia'] @entity1138 :: ('MESH:D016685', 'Chemical') :: ['mitomycin C'] @entity1139 :: ('CHEBI:27504', 'Chemical') :: ['MMC'] @entity6349 :: ('MESH:D004416', 'Disease') :: ['dysplastic']
@entity5 :: (MESH:D009369,Disease) :: ['squamous neoplasia']
