OBJECTIVE: To choose the best procedure on preparation of acellular @entity291 pericardium (ABP) guided bone regeneration (GBR) material. METHODS: The BP was decellularized with 0. 25% Trypsin + 0.5% Triton X-100. The acellular @entity291 pericardiums (ABPs) were treated with @entity1391 -buffered saline(PBS) (group A), 95% @entity653 (group B), EDAC (group C), and EDAC and 95% @entity653 (group D) respectively. The treated ABPs were implanted subcutaneously in the back of SD @entity35 respectively at random and no material was implanted as control. Seven @entity35 were sacrificed at 2 weeks, twelve at 4 weeks, twelve at 8 weeks, seven at 16 weeks. Local reaction was studied grossly. The amount of @entity1358 ( @entity1358 ) and the percentage of @entity528 were reckoned by images analysis system. RESULTS: The ABPs were replaced by fibroblasts completely in group A at 8 weeks, in group C at 16 weeks, but only less than 50% till 16 weeks in groups B and D. In all groups, the depth of surrounding fibres attenuated time-dependently. The @entity1358 amount of the groups B and D was higher than that of the control group, and the ABP of the groups B and D degraded partly at 16 weeks. CONCLUSION: The ABP treated with EDAC can be replaced by the surrounding tissues and has good biocompatibility.

[Experimental study on acellular XXXX pericardium guided bone regeneration material].

@entity653 :: ('MESH:D005990', 'Chemical') :: ['glycerol'] @entity1358 :: ('MESH:D002292', 'Disease') :: ['antigen presenting cell', 'APC'] @entity1391 :: ('CHEBI:26020', 'Chemical') :: ['phosphate'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity291 :: ('9913', 'Species') :: ['bovine'] @entity528 :: ('MESH:D009410', 'Disease') :: ['ABP degeneration']
@entity291 :: (9913,Species) :: ['bovine']
