The effect of diet composition on post-absorptive (15 h fast) @entity844 and @entity413 metabolism was investigated in five healthy @entity1 after 2 weeks on a low- @entity1735 (Low- @entity435 ) diet (30 % energy intake from @entity3363 , 55 % from fat, 15 % from protein) and after 2 weeks on a high- @entity1735 (High- @entity435 ) diet (energy intake 75, 10 and 15 % from @entity3363 , fat and protein respectively). The diets were isoenergetic and comprised real foods. Stable-isotope tracer methodology and indirect calorimetry were employed to measure @entity413 and @entity844 kinetics and oxidation. The relative contribution of @entity1735 to the total energy expenditure was significantly higher after the High- @entity435 diet. After the High- @entity435 diet, total and plasma fatty oxidation (2.4 (SE 0.7) and 2.1 (SE 0.4) micromol/kg per min respectively) were significantly lower than after the Low- @entity435 diet (4.8 (SE 0.5) and 4.6 (SE 0.8) micromol/kg per min for total and plasma fatty oxidation respectively). The rate of appearance (Ra) of non-esterified @entity1718 ( @entity3691 ) in plasma and the arterial @entity3691 concentration were both significantly lower following the High- @entity435 than the Low- @entity435 diet. However, even after the High- @entity435 diet, @entity3691 Ra was threefold higher than plasma @entity844 oxidation. Thus, the decrease in @entity844 oxidation after consumption of a high- @entity1735 diet for 2 weeks in healthy @entity1 is unlikely to result from decreased @entity844 delivery to the tissues. @entity413 Ra and arterial plasma @entity413 concentration were similar after the two diets. After the High- @entity435 diet, arterial @entity880 concentration was higher and total @entity1735 oxidation rate well exceeded @entity413 Ra in plasma. Therefore, alterations in intracellular mechanisms may limit @entity844 oxidation after high- @entity1735 diets.

Effect of isoenergetic low- and high- XXXX diets on substrate kinetics and oxidation in healthy @entity1 .

@entity1 :: ('9606', 'Species') :: ['men'] @entity844 :: ('CHEBI:35366', 'Chemical') :: ['fatty acid'] @entity3363 :: ('MESH:D002241', 'Chemical') :: ['carbohydrates'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['carbohydrate'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity3691 :: ('MESH:D005230', 'Chemical') :: ['NEFA'] @entity1718 :: ('MESH:D005227', 'Chemical') :: ['fatty acids'] @entity435 :: ('CHEBI:42485', 'Chemical') :: ['CHO'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose', 'Glucose']
@entity1735 :: (CHEBI:16646,Chemical) :: ['carbohydrate']
