A minority of @entity1 with @entity3686 develop @entity8 in their allografts after renal transplantation. Clinically, the @entity8 appears indistinguishable from @entity8 , in which the target of autoantibodies had been identified as the NC1 domain of the alpha 3 chain of type IV collagen, alpha 3(IV)NC1. However, in the majority of cases, @entity3686 is due to mutations in the gene encoding the alpha 5 chain of type IV collagen, located on the X chromosome. Neither chain is detectable in the @entity631 ( @entity631 ) of most @entity1 with @entity3686 . We investigated the targets of the alloantibodies of 12 @entity3686 @entity1 who developed post-transplant @entity8 by Western blotting onto recombinant NC1 domains made in insect cells. Binding to these antigens, for both typical @entity7860 and @entity3686 anti- @entity631 antibodies, was strong and conformation-sensitive. Nine antibodies showed selective binding to alpha 5(IV)NC1. This specificity was confirmed by the demonstration of binding to a 26 kDa band of collagenase-solubilized @entity1 @entity631 , and/or binding to normal epidermal as well as renal basement membranes by indirect immunofluorescence. One antibody showed binding to alpha 5 and alpha 3(IV)NC1, while two showed predominant binding to alpha 3(IV)NC1. All seven @entity1 whose pedigree or mutation analysis showed X-linked inheritance had predominant anti-alpha 5 reactivity. One with predominant anti-alpha 3 reactivity had a @entity19787 mutation. These findings show that human @entity8 can be associated with antibodies directed towards different molecular targets. Alpha 5(IV)NC1 is the primary target in most @entity1 with @entity3686 who develop post-transplant @entity8 .

Targets of alloantibodies in XXXX after renal transplantation.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity8 :: ('MESH:D007674', 'Disease') :: ['renal disease', "Goodpasture's disease of native kidneys", 'anti-GBM disease'] @entity3686 :: ('MESH:D009394', 'Disease') :: ['X-linked Alport syndrome', 'Alport', 'Alport syndrome'] @entity19787 :: ('1285', 'Gene') :: ['COL4A3'] @entity7860 :: ('MESH:D019867', 'Disease') :: ['Goodpasture'] @entity631 :: ('MESH:D005921', 'Disease') :: ['glomerular basement membrane', 'GBM']
@entity8 :: (MESH:D007674,Disease) :: ['Alport anti-glomerular basement membrane disease']
