A dynamic model of @entity413 overflow metabolism in batch and fed-batch cultivations of @entity23208 under fully aerobic conditions is presented. Simulation based on the model describes cell growth, respiration, and @entity598 formation as well as @entity598 reconsumption during batch cultures, the transition of batch to fed-batch culture, and fed-batch cultures. @entity526 excreted @entity598 only when specific @entity413 uptake exceeded a critical rate corresponding to a maximum respiration rate. In batch cultures where the @entity413 uptake was unlimited, the overflow @entity598 made up to 9. 0 +/- 1.0% @entity505 / @entity505 of the @entity413 consumed. The applicability of the model to dynamic situations was tested by challenging the model with @entity413 and @entity598 pulses added during the fed-batch part of the cultures. In the presence of a @entity413 feed, @entity526 utilized @entity598 3 times faster than in the absence of @entity413 . The cells showed no significant difference in maximum specific uptake rate of endogenous @entity598 produced by @entity413 overflow and exogenous @entity598 added to the culture, the value being 0.12-0.18 g g-1 h-1 during the entire fed-batch culture period. @entity598 inhibited the specific growth rate according to a noncompetitive model, with the inhibition constant (ki) being 9 g of @entity598 /L. This was due to the reduced rate of @entity413 uptake rather than the reduced yield of biomass.

Modeling of overflow metabolism in batch and fed-batch cultures of XXXX .

@entity598 :: ('CHEBI:47622', 'Chemical') :: ['Acetate', 'acetate'] @entity505 :: ('MESH:D002244', 'Chemical') :: ['carbon'] @entity23208 :: ('316407', 'Species') :: ['Escherichia coli W3110'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity526 :: ('562', 'Species') :: ['E. coli']
@entity526 :: (562,Species) :: ['Escherichia coli']
