@entity191 ( @entity191 ) is a @entity66 caused by both genetic and environmental factors. @entity8095 is of particular interest in @entity191 since it is a major component of Lewy bodies and mutations in the @entity8095 gene were identified in familial @entity191 . Oxidative stress and @entity409 are implicated in the pathogenesis of @entity191 but their interactions as well as their effect on aggregates formation are not yet clear. We therefore examined the roles of oxidative stress and proteasomal inhibition on protein aggregates induction in na  ve and neuronally differentiated @entity521 SH-SY5Y cells. @entity521 cells were stably transfected with @entity164 ( @entity164 ) and @entity20098 mutant @entity8095 . Na  ve and transfected cells were exposed to oxidative stress induced by @entity3788 , SIN-I, @entity37962 and to proteasomal inhibition by @entity16628 . Proteasomal inhibition caused a dose-dependent decrease in viability and induced protein aggregates formation containing @entity8095 and ubiquitin. Proteasomal inhibition induced significantly increased @entity8095 aggregation in cells expressing mutant @entity8095 . Exposure to reactive @entity26 species (ROS) combined with proteasomal inhibition increased aggregates formation. Inclusion body formation and cell death of differentiated @entity521 cells overexpressing @entity8095 can serve as a valuable model for elucidating the molecular components that cause @entity179 in @entity191 as well as evaluating pharmacological interventions.

Proteasomal inhibition hypersensitizes differentiated XXXX cells to oxidative damage.

@entity8095 :: ('6622', 'Gene') :: ['Alpha-synuclein', 'alpha-synuclein'] @entity66 :: ('MESH:D004194', 'Disease') :: ['multifactorial disease'] @entity3788 :: ('MESH:D012402', 'Chemical') :: ['rotenone'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity409 :: ('MESH:D008107', 'Disease') :: ['proteasomal dysfunction'] @entity191 :: ('MESH:D010300', 'Disease') :: ["Parkinson's disease", 'PD'] @entity37962 :: ('CHEBI:30812', 'Chemical') :: ['FeCl(2,)'] @entity179 :: ('MESH:D019636', 'Disease') :: ['neurodegeneration'] @entity164 :: ('MESH:D017827', 'Disease') :: ['WT', 'wild type'] @entity521 :: ('MESH:D009447', 'Disease') :: ['neuroblastoma', 'Neuroblastoma'] @entity20098 :: ('c|SUB|A|53|T', 'DNAMutation') :: ['A53T'] @entity16628 :: ('MESH:C067713', 'Chemical') :: ['lactacystin']
@entity521 :: (MESH:D009447,Disease) :: ['neuroblastoma']
