The use of autogenous venous graft with intraluminal injection of Schwann cells to enhance nerve regeneration of @entity1347 was evaluated in a @entity95 tibial nerve-repair model. Schwann cells were isolated from the excised @entity95 tibial nerve by using the @entity7055 differential adhesion method. The cultured cells were identified by immunocytochemical labeling for S-100 protein. @entity4957 in 4-cm segments were created in 24 animals, which were then divided into three groups. In Group 1, the @entity4957 was repaired with interposition vein graft alone; in Group 2, the nerve defect was repaired with a vein graft with intraluminal injection of Schwann-cell suspension; in Group 3, the nerve defect was repaired by autogenous nerve graft alone. At 2 months postoperatively, electrophysiologic evaluation showed that an evoked muscle action potential was recorded for the animals in Group 2, with vein grafting plus Schwann cells, and for those in Group 3, with autogenous nerve grafting, but not for those in Group 1, where vein grafting alone was used. The average motor nerve conduction velocity in the group with vein grafting and Schwann cells was 3.4 +/- 1.5 m/sec, which was slower than the nerve grafting group (7.8 +/- 1.8 m/sec). Histologic analysis confirmed there was formation of new nerve fascicles with myelination in the vein graft filled with Schwann cells. No nerve regrowth was found in the vein grafts without Schwann cells. These results suggested that isolated Schwann cells are able to survive in a vein graft, and that the vein graft with intraluminal seeded Schwann cells could be an alternative for repairing injured nerves with long gaps.

Autogenous venous graft with one-stage prepared Schwann cells as a conduit for repair of XXXX .

@entity1347 :: ('MESH:C537775', 'Disease') :: ['long segmental nerve defects'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity4957 :: ('MESH:D020429', 'Disease') :: ['tibial nerve defect', 'Tibial nerve defects'] @entity7055 :: ('MESH:D011107', 'Chemical') :: ['polylysine']
@entity1347 :: (MESH:C537775,Disease) :: ['long segmental nerve defects']
