INTRODUCTION: Delta-opioid receptor (DOR) and its endogenous ligands distribute in trigeminal system and play a very important role in modulating peripheral @entity158 . DOR activation can trigger p44/42 mitogen-activated protein kinase ( @entity2508 ) and Akt signaling pathways, which participate in anti-inflammatory and neuroprotective effects. In this study, our purpose was to determine the dynamic changes of DOR in @entity4494 ( @entity4494 ) neurons during the process of acute @entity32 and elucidate its possible mechanism. METHODS: Forty @entity35 were used to generate lipopolysaccharide-induced acute @entity11624 animal models at 6, 12, and 24  hours and sham-operated groups. @entity697 was confirmed by @entity1710 - @entity1711 staining, and @entity4494 neuron activation was determined by anti- @entity5019 immunohistochemistry. DOR protein and gene expression in @entity4494 was investigated by immunohistochemistry, Western blotting, and real-time polymerase chain reaction, and DOR expression in trigeminal nerves and dental pulp was also determined by immunohistochemistry. To further investigate the mechanism of DOR modulating acute @entity32 , the change of pErk1/2 and pAkt in @entity4494 was examined by immunohistochemistry. RESULTS: Lipopolysaccharide could successfully induce acute @entity11624 and activated @entity4494 neurons. @entity697 could dynamically increase DOR protein and gene expression at 6, 12, and 24  hours in @entity4494 , and DOR dimerization was significantly increased at 12 and 24  hours. @entity697 also induced the dynamic change of DOR protein in trigeminal nerve and dental pulp. Furthermore, @entity2508 and Akt signaling pathways were inhibited in @entity4494 after acute @entity11624 . CONCLUSIONS: Increased DOR expression and dimerization may play important roles in peripheral acute @entity158 .

Dynamic Regulation of Delta-Opioid Receptor in @entity35 Trigeminal Ganglion Neurons by Lipopolysaccharide-induced XXXX .

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation', 'dental pulp inflammation'] @entity697 :: ('MESH:D000208', 'Disease') :: ['Acute pulpitis'] @entity2508 :: ('50689;116590', 'Gene') :: ['ERK1/2'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity4494 :: ('MESH:D045888', 'Disease') :: ['TG', 'trigeminal ganglion'] @entity5019 :: ('314322(Tax:10116)', 'Gene') :: ['c-Fos'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hematoxylin'] @entity11624 :: ('MESH:D011671', 'Disease') :: ['pulpitis'] @entity158 :: ('MESH:D010146', 'Disease') :: ['inflammatory pain'] @entity35 :: ('10116', 'Species') :: ['rats']
@entity697 :: (MESH:D000208,Disease) :: ['Acute Pulpitis']
