The purpose of this study was to investigate the expression of @entity7 ( @entity7 ) -C in @entity1 @entity1721 to elucidate its role in lymph node @entity3 and @entity5 progression. The expression of @entity9961 and @entity7802 genes was examined in 5 @entity418 cell lines, 12 fresh biopsy specimens and 48 archival surgical specimens of @entity1 @entity418 tissues by RT-PCR and immunohistochemistry. Immunohistochemistry using antibodies against @entity2280 (endothelial cell specific) was also carried out and microvessels were quantified by counting vessels in a 200x field in the most vascular area of the @entity5 . Of the 5 @entity1 @entity418 cell lines, 4 constitutively expressed @entity9961 mRNA. In 8 (66.7%) of 12 cases, @entity9961 mRNA was detected in only @entity5 tissues but not in normal mucosa by RT-PCR. There was a significant relationship between @entity9961 and @entity7802 mRNA expression. Out of the 48 surgical specimens of @entity418 , 19 (39.6%) and 10 (20.8%) exhibited intense @entity9961 immunoreactivity in the cytoplasm of many @entity5 cells and the stromal cells, respectively. In contrast, @entity7802 was mainly expressed on the lymphatic endothelial cells. Normal and @entity6349 esophageal squamous epithelium exhibited no or faint cytoplasmic staining of @entity9961 . @entity9961 expression correlated with depth of @entity5 invasion, @entity5 stage, venous invasion, lymphatic invasion and lymph node @entity3 . Vessel count was significantly higher in the @entity9961 positive @entity5 than in the negative @entity5 . These results overall suggest that @entity9961 may play a role in @entity5 progression via lymphangiogenesis and angiogenesis in @entity1 @entity418 .

Clinicopathological significance of @entity7 ( @entity7 )-C in @entity1 XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity7802 :: ('2324', 'Gene') :: ['flt-4', 'Flt-4'] @entity7 :: ('7422', 'Gene') :: ['vascular endothelial growth factor', 'VEGF'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'tumors'] @entity9961 :: ('7424', 'Gene') :: ['VEGF-C'] @entity1721 :: ('MESH:C562729', 'Disease') :: ['esophageal squamous cell carcinomas'] @entity418 :: ('MESH:D004938', 'Disease') :: ['esophageal carcinomas', 'esophageal carcinoma'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity2280 :: ('947', 'Gene') :: ['CD34'] @entity6349 :: ('MESH:D004416', 'Disease') :: ['dysplastic']
@entity1721 :: (MESH:C562729,Disease) :: ['esophageal squamous cell carcinomas']
