Cell fusion during @entity375 mating provides a model for signaling-controlled changes at the cell surface. We identified the @entity33370 gene in a screen for genes required for cell fusion in both mating types during mating. @entity33370 is a @entity5681 -inducible gene required for axial bud site selection in haploid @entity375 and for proteolytic maturation of a-factor. Two other bud site selection genes, @entity18708 , encoding a small GTPase, and @entity70441 , were also required for efficient cell fusion. Based on double mutant analysis, @entity33370 in a MATalpha strain acted genetically in the same pathway with @entity70442 , a fusion-dedicated gene. Electron microscopy of @entity33370 , @entity18708 , and @entity70442 prezygotes revealed similar defects in nuclear migration, vesicle accumulation, cell wall degradation, and membrane fusion during cell fusion. The @entity33370 and @entity18708 mutants exhibited defects in @entity5681 -induced morphogenesis. @entity33370 protease function was required in MATalpha strains for fusion during mating. The ability of the @entity18708 p GTPase to cycle was required for efficient cell fusion, as it is for bud site selection. During conjugation, vegetative functions may be redeployed under the control of @entity5681 signaling for mating purposes. Since @entity18708 has been reported to physically associate with @entity16791 and @entity18710 components of the @entity5681 response pathway, we suggest that the bud site selection genes @entity18708 and @entity33370 may act to mediate @entity5681 control of @entity70442 -based fusion events during mating.

A role for a protease in morphogenic responses during XXXX cell fusion.

@entity16791 :: ('851190(Tax:4932)', 'Gene') :: ['Cdc24p'] @entity18710 :: ('852499(Tax:4932)', 'Gene') :: ['Bem1p'] @entity70441 :: ('850345(Tax:4932)', 'Gene') :: ['BUD3'] @entity70442 :: ('855272(Tax:4932)', 'Gene') :: ['Fus2p', 'FUS2', 'fus2'] @entity375 :: ('4932', 'Species') :: ['yeast'] @entity33370 :: ('856240(Tax:4932)', 'Gene') :: ['Axl1p', 'axl1', 'AXL1'] @entity18708 :: ('853056(Tax:4932)', 'Gene') :: ['Rsr1p', 'Rsr1', 'rsr1', 'RSR1'] @entity5681 :: ('CHEBI:26013', 'Chemical') :: ['pheromone']
@entity375 :: (4932,Species) :: ['yeast']
