BACKGROUND: @entity9052 has been shown to exert antistress effects both in vitro and in vivo. However, the effects of @entity9052 on stress in brain cells are not well understood. This study investigated how Korean Red @entity9052 (KRG) controls @entity273 -induced apoptosis via regulation of @entity4357 -3 kinase (PI3K)/Akt and @entity172 receptor (ER)-b signaling. METHODS: @entity1 @entity521 SK-N-SH cells were pretreated with KRG and subsequently exposed to @entity274 . The ability of KRG to inhibit oxidative stress-induced apoptosis was assessed in @entity98 @entity137 assays. Apoptotic protein expression was examined by Western blot analysis. The roles of ER-b, PI3K, and p-Akt signaling in KRG regulation of apoptosis were studied using small interfering RNAs and/or target antagonists. RESULTS: Pretreating SK-N-SH cells with KRG decreased expression of the proapoptotic proteins p-p53 and @entity522 , but increased expression of the antiapoptotic protein @entity588 . KRG pretreatment was also associated with increased ER-b, PI3K, and p-Akt expression. Conversely, ER-b inhibition with small interfering RNA or inhibitor treatment increased p-p53 and @entity522 levels, but decreased @entity588 , PI3K, and p-Akt expression. Moreover, inhibition of PI3K/Akt signaling diminished p-p53 and @entity522 levels, but increased @entity588 expression. CONCLUSION: Collectively, the data indicate that KRG represses oxidative stress-induced apoptosis by enhancing PI3K/Akt signaling via upregulation of ER-b expression.

Korean Red @entity9052 inhibits apoptosis in @entity521 cells via XXXX receptor b-mediated @entity4357 -3 kinase/Akt signaling.

@entity172 :: ('MESH:D004967', 'Chemical') :: ['estrogen'] @entity1 :: ('9606', 'Species') :: ['Human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H2O2'] @entity522 :: ('836', 'Gene') :: ['caspase-3'] @entity588 :: ('596', 'Gene') :: ['BCL2'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['hydrogen peroxide'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity521 :: ('MESH:D009447', 'Disease') :: ['neuroblastoma'] @entity9052 :: ('4054', 'Species') :: ['Ginseng', 'ginseng'] @entity4357 :: ('MESH:D010716', 'Chemical') :: ['phosphatidylinositol']
@entity172 :: (MESH:D004967,Chemical) :: ['estrogen']
