AKXD recombinant inbred (RI) strains develop a variety of @entity1778 and @entity706 due to somatically acquired insertions of retroviral DNA into the genome of hematopoetic cells that can mutate cellular proto-oncogenes and @entity5 suppressor genes. We generated a new set of @entity5 from nine AKXD RI strains selected for their propensity to develop @entity1892 , the most common type of @entity1 @entity5 . We employed a PCR technique called viral insertion site amplification (VISA) to rapidly isolate genomic sequence at the site of provirus insertion. Here we describe 550 VISA sequence tags (VSTs) that identify 74 common insertion sites (CISs), of which 21 have not been identified previously. Several suspected proto-oncogenes and @entity5 suppressor genes lie near CISs, providing supportive evidence for their roles in @entity5 . Furthermore, numerous previously uncharacterized genes lie near CISs, providing a pool of candidate disease genes for future research. Pathway analysis of candidate genes identified several signaling pathways as common and powerful routes to @entity5 , including Notch, E-protein, @entity1991 , and Ras signaling. Misregulation of several Notch signaling genes was confirmed by quantitative RT-PCR. Our data suggest that analyses of insertional mutagenesis on a single genetic background are biased toward the identification of cooperating mutations. This @entity5 collection represents the most comprehensive study of the genetics of @entity8624 and @entity706 development in @entity19 . We have deposited the VST sequences, CISs in a genome viewer, histopathology, and molecular @entity5 typing data in a public web database called VISION (Viral Insertion Sites Identifying Oncogenes), which is located at http://www. @entity19 -genome.bcm.tmc.edu/vision .

Retroviral insertions in the VISION database identify molecular pathways in XXXX @entity706 and @entity706 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity1991 :: ('18033(Tax:10090)', 'Gene') :: ['NFkappaB'] @entity706 :: ('MESH:D008223', 'Disease') :: ['lymphoma', 'lymphomas'] @entity5 :: ('MESH:D009369', 'Disease') :: ['blood cancer', 'tumor', 'cancer', 'hematopoietic cancers', 'tumors'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity1778 :: ('MESH:D007938', 'Disease') :: ['leukemias'] @entity1892 :: ('MESH:D016393', 'Disease') :: ['B-cell tumors'] @entity8624 :: ('MESH:D015448', 'Disease') :: ['B-cell leukemia']
@entity19 :: (10090,Species) :: ['mouse']
