Metabolic suppression by temperature is a key to successful organ preservation. Additional methods for inducing metabolic suppression may further improve organ preservation. Extracellular @entity520 has been shown to suppress warm @entity130 to various isolated cells. @entity520 may suppress enzymes with a pH optimum at the pH of the cytosol (pH 7.3). In this study, the combination of @entity2332 and @entity520 was used to determine if it would improve renal preservation. @entity235 kidneys were cold-stored (CS) for 48 h in University of Wisconsin (UW) solution with the pH adjusted to 6.4, 6.8, 7.4, or 7.8. Kidneys were also machine-perfused (MP) for 3 days with the @entity12742 perfusion solution (Belzer's machine perfusion solution, MPS) at pHs similar to those tested for CS. Renal function (serum @entity1011 , SCr) and survival were recorded in immediate contralateral nephrectomized recipients. On the basis of maximum SCr values, kidneys preserved by CS or MP were best preserved at pHs of 7.4 or 7.8. At a pH of 6.8, SCr values were elevated and returned to normal at a slower rate than in those preserved at higher pHs. This study shows that @entity520 is not cytoprotective to cold-stored @entity235 kidneys and causes preservation/reperfusion injury.

Optimal pH for simple cold storage or machine perfusion of XXXX kidneys with UW solution.

@entity235 :: ('9615', 'Species') :: ['dog', 'Dog'] @entity130 :: ('MESH:D014947', 'Disease') :: ['anoxic injury'] @entity520 :: ('MESH:D000138', 'Disease') :: ['acidosis', 'Acidosis'] @entity12742 :: ('MESH:C030691', 'Chemical') :: ['gluconate'] @entity1011 :: ('MESH:D003404', 'Chemical') :: ['creatinine'] @entity2332 :: ('MESH:D007035', 'Disease') :: ['hypothermia']
@entity235 :: (9615,Species) :: ['dog']
