UNASSIGNED: In microbial processes for production of proteins, biomass and @entity1568 -containing commodity chemicals, @entity855 requirements for @entity1568 assimilation affect product yields on the energy producing substrate. In @entity375 , a current host for heterologous protein production and potential platform for production of @entity1568 -containing chemicals, uptake and assimilation of @entity3104 requires 1 @entity855 per incorporated @entity3655 . @entity1133 assimilation by this @entity375 is more energy efficient but still requires 0.5 @entity855 per @entity3655 produced. To decrease @entity855 costs for @entity1568 assimilation, the @entity375 gene encoding @entity855 -dependent urease ( @entity57716 ) was replaced by a @entity6739 gene encoding @entity855 -independent urease (ure2), along with its accessory genes ureD, ureF and ureG. Since @entity6739 ure2 is a @entity3411 -dependent enzyme and @entity375 does not express native @entity3411 -dependent enzymes, the @entity6739 high-affinity @entity3406 -transporter gene (nic1) was also expressed. Expression of the @entity6739 genes into @entity57716   @entity375 yielded an in vitro @entity855 -independent urease activity of 0.44  0.01  molmin(-1)mg protein(-1) and restored growth on @entity1133 as sole @entity1568 source. Functional expression of the Nic1 transporter was essential for growth on @entity1133 at low @entity3411 concentrations. The maximum specific growth rates of the engineered strain on @entity1133 and @entity3104 were lower than those of a @entity57716 reference strain. In @entity413 -limited chemostat cultures with @entity1133 as @entity1568 source, the engineered strain exhibited an increased release of @entity3265 and reduced @entity1568 content of the biomass. Our results indicate a new strategy for improving @entity375 -based production of @entity1568 -containing chemicals and demonstrate that @entity3411 -dependent enzymes can be functionally expressed in @entity375 .

Functional expression of a heterologous @entity3406 -dependent, @entity855 -independent urease in XXXX .

@entity3406 :: ('MESH:D009532', 'Chemical') :: ['nickel'] @entity6739 :: ('4896', 'Species') :: ['Schizosaccharomyces pombe', 'S. pombe'] @entity1568 :: ('MESH:D009584', 'Chemical') :: ['nitrogen'] @entity1133 :: ('MESH:D014508', 'Chemical') :: ['Urea', 'urea'] @entity3265 :: ('MESH:D000641', 'Chemical') :: ['ammonia'] @entity3655 :: ('CHEBI:16134', 'Chemical') :: ['NH3'] @entity57716 :: ('852507(Tax:4932)', 'Gene') :: ['DUR1,2', 'dur1,2'] @entity3411 :: ('CHEBI:49786', 'Chemical') :: ['Ni(2+)'] @entity3104 :: ('MESH:D000644', 'Chemical') :: ['ammonium'] @entity375 :: ('4932', 'Species') :: ['yeast', 'Saccharomyces cerevisiae', 'S. cerevisiae'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity375 :: (4932,Species) :: ['Saccharomyces cerevisiae']
