Metabolic changes that contribute to differentiation are not well understood. Overwhelming evidence shows the critical role of glycolytic enzyme @entity2388 kinase ( @entity24525 ) in directing metabolism of proliferating cells. However, its role in metabolism of differentiating cells is unclear. Here we studied the role of @entity24525 in @entity1359 ( @entity4515 )-induced megakaryocytic differentiation in @entity1 @entity1778 K562 cells. We observed that @entity4515 treatment decreased @entity5 but increased @entity855 production, along with up-regulated expression of two @entity24525 isoforms ( @entity17341 and @entity24525 ) in an @entity3963 -dependent manner. Interestingly, silencing of @entity24525 ( @entity17341 and @entity24525 ) inhibited @entity4515 -induced megakaryocytic differentiation, as revealed by decreased expression of megakaryocytic differentiation marker @entity1428 and cell cycle behavior. Further, @entity4515 -induced @entity855 production reduced greatly upon @entity24525 silencing, suggesting that @entity24525 is required for @entity855 synthesis. In addition to metabolic effects, @entity4515 treatment also translocated @entity17341 , but not @entity24525 , into nucleus. @entity1366 knockdowns independently and together suggested the role of @entity3963 in the up-regulation of both the isoforms of @entity24525 , proposing a role of @entity3963 - @entity24525 isoform axis in differentiation. Collectively, our findings unravel @entity3963 guided @entity24525 -dependent metabolic changes during @entity4515 induction, which are important in megakaryocytic differentiation.

XXXX -Pyruvate Kinase Axis Permits @entity1359 -induced Megakaryocyte Differentiation in K562 Cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer-type anabolic metabolism'] @entity3963 :: ('5594', 'Gene') :: ['ERK2'] @entity4515 :: ('CHEBI:60755', 'Chemical') :: ['PMA'] @entity1359 :: ('CHEBI:37537', 'Chemical') :: ['phorbol 12-myristate 13-acetate'] @entity17341 :: ('5315', 'Gene') :: ['PKM2'] @entity24525 :: ('5313', 'Gene') :: ['PKR', 'PK'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP'] @entity1778 :: ('MESH:D007938', 'Disease') :: ['leukemia'] @entity1428 :: ('3690', 'Gene') :: ['CD61'] @entity1366 :: ('5595;5594', 'Gene') :: ['ERK1/2'] @entity2388 :: ('MESH:D019289', 'Chemical') :: ['pyruvate']
@entity3963 :: (5594,Gene) :: ['ERK2']
