To characterize the defect in glomerular permeability leading to @entity633 in @entity35 made @entity1985 acutely by infusion of @entity9850 (HDM) or chronically by administration of @entity632 ( @entity632 ) (Adria), we developed and validated a tissue accumulation method for simultaneous determination of the glomerular sieving coefficients (GSC) of anionic 131I-labeled @entity291 albumin (BSA-pI 4.9) and 125I-labeled charge-modified neutral BSA (nBSA-pI 7.5 to 8.0). Total filtered marker was calculated by adding marker excreted in the urine to that filtered but reabsorbed by the tubules. The latter was determined by subtracting interstitial marker present in the left kidney, rendered non-filtering by ureteral ligation during @entity2656 diuresis, from the total marker accumulating within the right, filtering kidney. Experiments showed that markers circulated and were excreted intact and were neither degraded nor deiodinated during the period of the clearance studies. In control animals the GSC of nBSA (0.026 +/- 0.004) greatly exceeded that of BSA (0.0006 +/- 0.0002), demonstrating the normal charge dependence of permeability. Both proteinuric groups had marked increases in the GSC of BSA (HDM: 0.021 +/- 0.005; Adria: 0.025 +/- 0.004), which correlated with appearance of @entity35 albumin in their urine. HDM @entity35 also had a twofold increase in the GSC of nBSA (0.049 +/- 0.005), indicating alteration of the size dependence of permeability. The absolute increase of GSC of BSA and nBSA was similar, suggesting that @entity633 resulted from appearance of new "pores" in the glomerular filter that were not charge selective for proteins of the size of albumin. Thus, infusion of HDM, which binds to and neutralizes GBM anions, appears to produce @entity633 by inducing a structural change in the glomerular filter. Conversely, Adria @entity35 had no significant increase in the GSC of nBSA (0.031 +/- 0.005), indicating no significant change in the size dependence of permeability for proteins of the size of albumin. In these animals, the GSC of the anionic BSA approached that of the neutral nBSA, indicating that @entity632 induces @entity633 by markedly reducing the normal charge dependence of permeability.

Glomerular sieving of anionic and neutral @entity291 albumins in proteinuric XXXX .

@entity632 :: ('MESH:D004317', 'Chemical') :: ['Adriamycin', 'doxorubicin'] @entity2656 :: ('MESH:D008353', 'Chemical') :: ['mannitol'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity633 :: ('MESH:D000419', 'Disease') :: ['albuminuria'] @entity9850 :: ('MESH:D006583', 'Chemical') :: ['hexadimethrine'] @entity1985 :: ('MESH:D009404', 'Disease') :: ['nephrotic'] @entity291 :: ('9913', 'Species') :: ['bovine']
@entity35 :: (10116,Species) :: ['rats']
