UNASSIGNED: @entity1 endometrial stem cells (hEnSCs) are a new source of adult multipotent stem cells with the ability of differentiation into many cell lineages. Many stem cell sources are desirable for differentiation into Schwann cells. Schwann-like cells derived from hEnSCs may be one of the ideal alternative cell sources for Schwann cell generation. In this study, for differentiation of hEnSCs into Schwann cells, hEnSCs were induced with @entity309 /FSK/PDGF-AA/HRG as an induction medium for 14  days. The cells were cultured in a @entity3145 ( @entity3145 ) and fibrin gel matrix. The viability of cultured cells in the fibrin gel and @entity3145 was analyzed with @entity40724 ( @entity98 ) assay for 7  days. The attachment of cells was analyzed with SEM and @entity14939 staining. The expression of S100 and P75 as Schwann cell markers was evaluated by immunocytochemistry and quantitative real-time PCR (RT-PCR). The evaluation of the @entity98 assay and gene expression showed that the survival rate and differentiation of hEnSCs into Schwann cells in the fibrin gel were better than those in the @entity3145 group. These results suggest that @entity1 EnSCs can be differentiated into Schwann cells in the fibrin gel better than in the @entity3145 , and the fibrin gel might provide a suitable three-dimensional (3D) scaffold for clinical applications for cell therapy of the nervous system.

Differentiation of XXXX Endometrial Stem Cells into Schwann Cells in Fibrin Hydrogel as 3D Culture.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity3145 :: ('MESH:D017695', 'Disease') :: ['TCP', 'tissue culture plate'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity40724 :: ('MESH:C000598529', 'Chemical') :: ['3-[4,5-dimethyl-2-thia-zolyl]-2, 5-diphenyl-2H-tetrazolium bromide'] @entity14939 :: ('MESH:C007293', 'Chemical') :: ['DAPI'] @entity309 :: ('MESH:D001172', 'Disease') :: ['RA']
@entity1 :: (9606,Species) :: ['Human']
