In previous studies, we described a soluble @entity141 /calmodulin-dependent protein kinase which is the major @entity141 /calmodulin-dependent microtubule-associated protein 2 ( @entity19744 ) kinase in @entity35 brain [Schulman, H. (1984) J. Cell Biol. 99, 11-19; Kuret, J. A., _ Schulman, H. (1984) Biochemistry 23, 5495-5504]. We now demonstrate that this protein kinase has broad substrate specificity. Consistent with a multifunctional role in cellular physiology, we show that in vitro the enzyme can phosphorylate numerous substrates of both neuronal and nonneuronal origin including @entity7229 , @entity30300 , @entity12148 , glycogen synthase, and myosin light chains. We have used @entity19744 to purify the enzyme from @entity35 lung and show that the brain and lung kinases have nearly indistinguishable physical and biochemical properties. A @entity141 /calmodulin-dependent protein kinase was also detected in @entity35 heart, @entity35 spleen, and in the ring ganglia of the marine mollusk @entity11066 . Partially purified @entity19744 kinase from each of these three sources displayed endogenous phosphorylation of a 54 000-dalton protein. Phosphopeptide analysis reveals a striking homology between this phosphoprotein and the 53 000-dalton autophosphorylated subunit of the major @entity35 brain @entity141 /calmodulin-dependent protein kinase. The enzymes phosphorylated @entity19744 , @entity12148 , and @entity7229 at peptides that are identical with those phosphorylated by the @entity35 brain kinase. This enzyme may be a multifunctional @entity141 /calmodulin-dependent protein kinase with a widespread distribution in nature which mediates some of the effects of @entity141 on microtubules, intermediate filaments, and other cellular constituents in brain and other tissues.

XXXX /calmodulin-dependent microtubule-associated protein 2 kinase: broad substrate specificity and multifunctional potential in diverse tissues.

@entity35 :: ('10116', 'Species') :: ['rat'] @entity30300 :: ('29304(Tax:10116)', 'Gene') :: ['ribosomal protein S6'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+'] @entity12148 :: ('24949(Tax:10116)', 'Gene') :: ['synapsin I'] @entity19744 :: ('25595(Tax:10116)', 'Gene') :: ['MAP-2'] @entity7229 :: ('81818(Tax:10116)', 'Gene') :: ['vimentin'] @entity11066 :: ('6500', 'Species') :: ['Aplysia californica']
@entity141 :: (CHEBI:29108,Chemical) :: ['Ca2+']
