UNASSIGNED: Cry j1 is the major peptide allergen of @entity12261 (Sugi), @entity12261 . Since some allergens disrupt epidermal permeability barrier homeostasis, we hypothesized that Cry j1 might have a similar effect. Intracellular @entity460 level in cultured @entity1 keratinocytes was measured with a ratiometric fluorescent probe, @entity593 AM. Application of Cry j1 significantly increased the intracellular @entity460 level of keratinocytes, and this increase was inhibited by @entity22152 or a @entity12610 ( @entity12610 ) antagonist. We found that Cry j1 itself did not show protease activity, but application of Cry j1 to cultured keratinocytes induced a rapid (within 30  s) and transient increase of protease activity in the medium. This transient increase was blocked by @entity22152 or @entity12610 antagonist. The effect of Cry j1 on transepidermal water loss (TEWL) of cultured @entity1 skin was measured in the presence and absence of a @entity22152 and @entity12610 antagonist. Cry j1 significantly impaired the barrier function of @entity1 skin ex vivo, and this action was blocked by co-application of @entity22152 or @entity12610 antagonist. Our results suggested that interaction of Cry j1 with epidermal keratinocytes leads to the activation of @entity12610 , which induces elevation of intracellular @entity460 and disruption of barrier function. Blocking the interaction of Cry j1 with epidermal keratinocytes might @entity56 and prevent disruption of epidermal permeability barrier homeostasis.

@entity12261 ( @entity12261 ) pollen allergen induces elevation of intracellular XXXX in @entity1 keratinocytes and impairs epidermal barrier function of @entity1 skin ex vivo.

@entity12261 :: ('3369', 'Species') :: ['Japanese cedar', 'Cryptomeria japonica'] @entity1 :: ('9606', 'Species') :: ['human'] @entity22152 :: ('83716', 'Gene') :: ['trypsin inhibitor'] @entity593 :: ('MESH:D016257', 'Chemical') :: ['Fura-2'] @entity56 :: ('MESH:D004342', 'Disease') :: ['ameliorate allergic reaction'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity12610 :: ('2150', 'Gene') :: ['PAR-2', 'protease-activated receptor 2']
@entity460 :: (MESH:D002118,Chemical) :: ['calcium']
