OBJECTIVE: To study the effects of prolactin (PRL) on HLA-DR and @entity2936 expressions by @entity1 thyrocytes, and to investigate the possible mechanisms for PRL to affect the development of @entity2743 ( @entity2743 ). METHODS: Thyrocytes in secondary culture, which were from @entity2743 thyroid glands and the tissues adjacent to the lesions of @entity1376 and @entity652 (control group), were treated respectively with ovine PRL (oPRL), @entity379 ( @entity379 ), interlukin-4 (IL-4) and oPRL plus @entity379 (10 U/ml) or IL-4 (5 ng/ml) for 7 days. HLA-DR and @entity2936 expressions on the thyrocytes were determined by immunofluorescent staining and flow cytometry. RESULTS: oPRL (12.5 ng/ml-1000 ng/ml) had no significant direct effect on HLA-DR or @entity2936 expression. It did not significantly affect the stimulation of HLA-DR expressions on the two groups of thyrocyte treated with @entity379 or on @entity2743 thyrocytes treated with IL-4. oPRL could antagonize the stimulation of @entity2936 expressions by @entity379 and the inhibition by IL-4 on both groups of thyrocytes. The antagonizing effects were related to the concentrations of PRL. @entity379 -stimulated percentages of @entity2936 + thyrocytes and delta mean fluorescence intensity (dMF) in both thyrocyte sources were significantly reduced in the presence of 200 ng/ml oPRL (both @entity2743 and CONTROL: P < 0.01 and P < 0.05, respectively; CONTROL: P < 0.05) and 1000 ng/ml oPRL ( @entity2743 : P < 0.01; CONTROL: P < 0.05). oPRL caused significant increasing in IL-4-inhibited percentages of @entity2936 + cells from the two groups of thyrocytes at 12.5 ng/ml and 1000 ng/ml and dMF from @entity2743 thyrocytes at 1000 ng/ml (P < 0.05). CONCLUSIONS: PRL can exert indirect effects on @entity2936 expressions on thyrocytes by antagonizing the modulatory actions of @entity379 and IL-4 with dose-related effects. This may be one important mechanism for PRL to affect the development of @entity2743 .

Effects of prolactin on HLA-DR and @entity2936 expressions by XXXX thyrocytes.

@entity1 :: ('9606', 'Species') :: ['human'] @entity2743 :: ('MESH:D006111', 'Disease') :: ["Graves' disease", 'GD'] @entity2936 :: ('958', 'Gene') :: ['CD40'] @entity1376 :: ('MESH:C564546', 'Disease') :: ['multinodular goiter'] @entity379 :: ('3458', 'Gene') :: ['interferon-gamma', 'IFN-gamma'] @entity652 :: ('MESH:D000236', 'Disease') :: ['adenoma']
@entity1 :: (9606,Species) :: ['human']
