OBJECTIVE: To study the feasibility and the diagnostic and prognostic interest of automated analysis of 1p, 19q, 9p and 10q status by FISH technique in @entity5 . METHODS: We analyzed a retrospective series of 33 consecutive @entity11 with oligodendroglial histology (originally diagnosed as 24 @entity3271 and 9 @entity816 ). For all cases, automated FISH analysis of 1p, 19q, 9p and 10q status were performed and compared to clinical and histological data, @entity8171 , IDH1R132H and alpha-internexin status (studied by immunohistochemistry) and overall survival (OS). Manual analysis of 9p and 10q status were also performed and compared to automated analysis to verify the concordance of the two methods. RESULTS: The 33 @entity11 were reclassified into 13 low-grade @entity3271 ( @entity3271 ), 10 @entity3271 (OIII), 3 diffuse @entity816 (AII), 3 @entity816 (AIII) and 4 @entity463 ( @entity463 ) according to the WHO 2016 histological criteria. The 1p and/or 19q imbalanced status were restricted to @entity816 with no correlation to their grade or their OS. Chromosome 9p deletion was restricted to OIII (70%) and @entity463 (100%) and was correlated with a shorter OS in the total cohort (p = 0.0007), the @entity3271 cohort (p = 0.03) and the @entity816 cohort (p = 0.001). Concordance between 9p manual and automated analysis was satisfactory (81%, k = 0.69). Chromosome 10q deletion was restricted to GBMs (50%) and was correlated with a poor OS in both the total cohort (p = 0.003) and the @entity816 ( @entity816 ) cohort (p = 0.04). Concordance between manual and automated analysis was satisfactory (79%, k = 0.62). CONCLUSION: Automated analysis of 1p, 19q, 9p and 10q status by FISH is a reliable technique which allows for refined classification of @entity5 . 1p and/or 19q imbalanced status is evidence of astrocytic differentiation. 9p deletion is found in high grade @entity3271 and @entity816 with a poor OS. 10q is related to @entity463 status and a poor OS.

Contribution of 1p, 19q, 9p and 10q Automated Analysis by FISH to the Diagnosis and Prognosis of XXXX According to WHO 2016 Guidelines.

@entity8171 :: ('546', 'Gene') :: ['ATRX'] @entity5 :: ('MESH:D009369', 'Disease') :: ['oligodendroglial tumors'] @entity11 :: ('MESH:D005910', 'Disease') :: ['gliomas'] @entity816 :: ('MESH:D001254', 'Disease') :: ['anaplastic astrocytomas', 'oligoastrocytomas', 'AS', 'astrocytoma', 'astrocytomas'] @entity463 :: ('MESH:D005909', 'Disease') :: ['glioblastomas', 'GBM'] @entity3271 :: ('MESH:D009837', 'Disease') :: ['oligodendrogliomas', 'anaplastic oligodendrogliomas', 'oligodendroglioma', 'OII']
@entity5 :: (MESH:D009369,Disease) :: ['Oligodendroglial Tumors']
