@entity1 blood mononuclear leukocytes exposed in vitro to perfluorochemical blood substitutes (Fluosol-DA and @entity37553 ) generated increased procoagulant activity that was time dependent. Mononuclear leukocytes incubated with 10 percent Fluosol-DA for 4 hours generated 3.43-fold more procoagulant activity than control cells. At 24 hours of incubation with 10 percent Fluosol-DA, cells generated 10.49-fold more procoagulant than control. Cells incubated with 10 or 20 percent @entity37553 generated 2.5- or 3.4-fold greater procoagulant than controls, respectively. The perfluorochemical emulsifier ( @entity12185 ) also stimulated 3.4-fold more activity than control cells. Stimulated oxidative metabolism ( @entity224 anion generation) was significantly impaired by Fluosol-DA but not by @entity37553 or @entity12185 . No significant perfluorochemical-induced @entity137 was measured by @entity3460 dye exclusion or @entity880 dehydrogenase release. Electron microscopic analysis showed progressive uptake of the perfluorochemicals by monocytes but not by lymphocytes. Thus, perfluorochemicals may differentially activate cellular initiators of @entity3459 while impairing other metabolic responses of mononuclear phagocytes. @entity1 receiving perfluorochemical preparations should be monitored for @entity854 and for disorders of the mononuclear phagocyte system.

Perfluorochemical blood substitutes differentially alter XXXX monocyte procoagulant generation and oxidative metabolism.

@entity1 :: ('9606', 'Species') :: ['Patients', 'Human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity37553 :: ('MESH:C008807', 'Chemical') :: ['FC-43'] @entity3459 :: ('MESH:D025861', 'Disease') :: ['coagulation'] @entity854 :: ('MESH:D018376', 'Disease') :: ['abnormalities of hemostasis'] @entity3460 :: ('MESH:D014343', 'Chemical') :: ['trypan blue'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity224 :: ('MESH:D013481', 'Chemical') :: ['superoxide'] @entity12185 :: ('MESH:D020442', 'Chemical') :: ['Pluronic F68']
@entity1 :: (9606,Species) :: ['human']
