Previous studies have revealed that @entity558 can activate transcription of the @entity1618 gene promoter through an @entity10591 ( @entity10591 ) binding site in @entity19 macrophage RAW264.7. In this study, we determined that, in addition to @entity10591 , @entity19775 and delta were also involved in @entity558 -induced gene expression of @entity1618 . By transient transfection with 5'-deletion mutants of the @entity1618 promoter, we found that there were two @entity558 -responsive elements in the promoter of the @entity19 @entity1618 gene. Analysis of these two regions by gel shift assay suggested that @entity10591 and @entity19775 and delta were bound to these two regions, respectively. By site-directed mutagenesis, we found that disruption at both the @entity10591 and C/EBP binding sites almost completely blocked the @entity558 response. By gel shift assay and Western blotting, we found that the DNA binding complex and protein expression of @entity19775 and delta were increased by @entity558 treatment, but these results were not found for @entity10591 . Overexpression of @entity19775 or @entity44758 , respectively, activated the promoter of the @entity1618 gene, and they were enhanced by @entity558 . Coimmunoprecipitation experiments in intact cells indicated that @entity558 stimulated interaction between @entity10591 and @entity19775 and delta. These results suggested that the interaction between @entity10591 and @entity19775 and delta induced by @entity558 cooperatively activated expression of the @entity1618 gene. The increase of @entity19775 and delta proteins and the enhancement of transactivation activity of @entity19775 and delta by @entity558 treatment, at least in part, explain the activation of @entity1618 gene expression.

Functional cooperation of simian virus 40 promoter factor 1 and CCAAT/enhancer-binding protein beta and delta in lipopolysaccharide-induced gene activation of @entity1618 in XXXX macrophages.

@entity558 :: ('MESH:C536528', 'Disease') :: ['LPS'] @entity19775 :: ('12608(Tax:10090)', 'Gene') :: ['C/EBPbeta'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity1618 :: ('16153(Tax:10090)', 'Gene') :: ['IL-10'] @entity10591 :: ('20683(Tax:10090)', 'Gene') :: ['Sp1', 'SV40 promoter factor 1'] @entity44758 :: ('12609(Tax:10090)', 'Gene') :: ['C/EBPdelta']
@entity19 :: (10090,Species) :: ['mouse']
