One possible way to expand the @entity1 heart donor pool is to include non-heart-beating @entity1 donors. To begin validating this approach, we developed an ex vivo cardiac perfusion circuit to support large @entity1 hearts in Langendorff mode and beating-ejecting mode and to assess and improve their ischemic tolerance. In vivo hemodynamic data and heparinized blood (4.0 +/- 0.5 L) were collected from 6 anesthetized @entity1707 . Hearts were isolated and connected to a recirculating perfusion circuit primed with autologous buffered blood (pH, 7.40). After retrograde aortic perfusion in Langendorff mode, the left atrium was gravity-filled at 10-20 mmHg, and the left ventricle began to eject against a compliance chamber in series with a systemic reservoir set to a hydraulic afterload of 100-120 mmHg. Left ventricular function was restored and maintained in all 6 hearts for 30 min. Cardiac output, myocardial @entity26 consumption, @entity64 work, aortic pressure, left atrial pressure, and heart rate were measured. The mean myocardial @entity26 consumption was 4.8 +/- 2.7 mL/min/100 g (95.8% of in vivo value); and mean @entity64 work, 5.3 +/- 1.1 g x m/100 g (58.95% of in vivo value). One resuscitated heart was exposed to 30 min of normothermic @entity63 , then flushed with Celsior and re-resuscitated. The ex vivo perfusion method described herein restored left ventricular ejection function and allowed assessment of ischemic tolerance in large @entity1 hearts, potentially a 1st step toward including non-heart-beating @entity1 donors in the @entity1 donor pool.

Ex vivo resuscitation of adult XXXX hearts.

@entity1 :: ('9606', 'Species') :: ['mammalian', 'human'] @entity1707 :: ('9823', 'Species') :: ['pigs'] @entity64 :: ('MESH:D020521', 'Disease') :: ['stroke'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemic arrest'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen']
@entity1707 :: (9823,Species) :: ['pig']
