Purpose. This study aimed to determine the dynamic changes of @entity4026 -related microRNAs (miRNAs) and cytokines over the course of experimental autoimmune anterior @entity1596 (EAAU) and elucidate the possible immunopathogenesis. Materials and Methods. @entity1596 was induced in Lewis @entity35 using @entity291 melanin-associated antigen. The inflammatory activity of the anterior chamber was clinically scored, and leukocytes in the @entity7462 were quantified. RNA was extracted from the iris/ciliary bodies and popliteal lymph nodes to reveal the dynamic changes of eight target miRNAs (miR-155-5p, miR-146a-5p, miR-182-5p, miR-183-5p, miR-147b, @entity69304 , @entity23803 , and miR-223-3p) and six cytokine mRNAs (IFN-y, @entity7940 , @entity69305 , @entity3426 , @entity568 , and @entity13358 ). In situ hybridization of miRNA and enzyme-linked immunosorbent assay quantification of cytokines were performed to confirm the results. Results. Disease activity and leukocyte quantification were maximum at day 15 after immunization. The profiling of miRNA revealed downregulation of miR-146a-5p, miR-155-5p, miR-223-3p, and miR-147b and upregulation of miR-182-5p, miR-183-5p, and @entity23803 . Cytokine analysis revealed IFN-y, @entity7940 , @entity69305 , @entity3426 , and @entity568 overexpression, with @entity13358 downregulation. Conclusions. Dynamic changes of miRNAs were observed over the course of EAAU. By initiating @entity4026 signaling, the expressions of downstream cytokines and effector cells from the Th17 and Th1 lineages were sequentially activated, contributing to the disease.

Expression of MicroRNAs in the Eyes of Lewis XXXX with Experimental Autoimmune Anterior @entity1596 .

@entity69304 :: ('100314074(Tax:10116)', 'Gene') :: ['miR-21-5p'] @entity7940 :: ('301289(Tax:10116)', 'Gene') :: ['IL-17'] @entity69305 :: ('84405(Tax:10116)', 'Gene') :: ['IL-12A'] @entity13358 :: ('25325(Tax:10116)', 'Gene') :: ['IL-10'] @entity568 :: ('24498(Tax:10116)', 'Gene') :: ['IL-6'] @entity1596 :: ('MESH:D014605', 'Disease') :: ['Uveitis', 'uveitis'] @entity7462 :: ('MESH:C562390', 'Disease') :: ['aqueous humor'] @entity23803 :: ('100314154(Tax:10116)', 'Gene') :: ['miR-9-3p'] @entity3426 :: ('24494(Tax:10116)', 'Gene') :: ['IL-1b'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity4026 :: ('81736(Tax:10116)', 'Gene') :: ['NF-kB'] @entity291 :: ('9913', 'Species') :: ['bovine']
@entity35 :: (10116,Species) :: ['Rats']
