@entity1735 ligands for lymphocyte @entity1196 are expressed on high endothelial venules (HEVs) in peripheral lymph nodes and sites of chronic @entity32 and mediate the recruitment of lymphocytes from the blood into these tissues. In the @entity19 , these ligands, collectively termed the peripheral lymph node addressin (PNAd), have been shown to contain @entity3953 , @entity3167 , and @entity597 and to include several @entity4973 glycoproteins including GlyCAM-1, @entity2280 , and MAdCAM-1. Monoclonal antibody (MAb) @entity12688 , which binds a @entity597 -dependent epitope, recognizes PNAd in both @entity19 and @entity1 . In @entity1 , only @entity2280 has been identified among the glycoprotein species that react with @entity12688 . Although @entity8729 is highly expressed in tonsil HEVs, it was not found to react with @entity12688 or to support @entity1196 -mediated lymphocyte rolling. To further characterize @entity1 PNAd, MAbs were developed against purified PNAd immunoisolated from @entity1 tonsil. MAbs JG-1, JG-5, JG-9, and JG-10, like @entity12688 , bind HEVs in @entity1 tonsil and react similarly in Western blots, and JG-9 and JG-10 also block lymphocyte rolling on purified PNAd. In addition, by competitive ELISA on purified tonsil PNAd, all MAbs were found to react with overlapping epitopes. However, JG-1, JG-5, JG-9, and JG-10 do not recognize @entity19 PNAd, and unlike @entity12688 , they recognize determinants that are sensitive to neuraminidase. Strikingly, the epitope recognized by JG-1, although abundant in tonsil and peripheral lymph node, is absent from appendix @entity2101 in some samples of chronically inflamed skin, even though these HEVs are @entity12688 reactive. Moreover, although JG-5 and JG-9 react well with tonsil, peripheral lymph node, and inflamed skin HEVs, they react only with occasional endothelial cells in appendix tissues. These findings point to significant diversity in the @entity1735 determinants expressed by HEVs and recognized by @entity1196 and demonstrate their differential representation in different sites in vivo. These antibodies should be useful in probing the precise structure of @entity1 @entity1196 ligands.

Complexity and differential expression of @entity1735 epitopes associated with XXXX recognition of high endothelial venules.

@entity1 :: ('9606', 'Species') :: ['humans', 'human', 'man'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity3167 :: ('MESH:D019158', 'Chemical') :: ['sialic acid'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity12688 :: ('MESH:C069886', 'Chemical') :: ['MECA-79'] @entity1735 :: ('CHEBI:16646', 'Chemical') :: ['Carbohydrate', 'carbohydrate'] @entity1196 :: ('6402', 'Gene') :: ['L-selectin'] @entity3953 :: ('MESH:D005643', 'Chemical') :: ['fucose'] @entity8729 :: ('6403', 'Gene') :: ['P-selectin'] @entity2101 :: ('MESH:C538394', 'Disease') :: ['HEVs or HEVs'] @entity2280 :: ('947', 'Gene') :: ['CD34'] @entity4973 :: ('42005', 'Species') :: ['HEV'] @entity597 :: ('CHEBI:16189', 'Chemical') :: ['sulfate']
@entity1196 :: (6402,Gene) :: ['L-selectin']
