PURPOSE: The overall aim of this study was to develop a novel treatment for @entity14 based on the use of gene therapy. Genetic modification of T lymphocytes has been used to specifically target and kill @entity5 cell lines directly. To test the efficacy of this method with clinically relevant materials, this study investigated the potential of T lymphocytes derived from @entity1 with advanced @entity14 to target autologous primary @entity5 material. METHODS T lymphocytes isolated preoperatively were modified genetically with recombinant retroviruses encoding @entity12711 -based chimeric immune receptors and were tested for functional activity against freshly isolated autologous @entity5 cells harvested from hepatic colorectal metastases. RESULTS: @entity1 -derived T cells were successfully transduced, and chimeric immune receptor expression was confirmed. Carcinoembryonic antigen expression on freshly isolated @entity14 cells was also demonstrated by molecular and immunohistochemical techniques. T cells expressing the anticarcinoembryonic antigen receptor were specifically activated by coculture with disaggregated or intact, diced @entity5 , whereas control non-carcinoembryonic antigen-targeted T-cell populations failed to activate. CONCLUSIONS: These results indicate that gene-targeted primary T lymphocytes depict specific functional activity against autologous @entity14 cells. This evidence indicates that chimeric immune receptor-expressing T cells may be able to circumvent the mechanisms used by @entity5 cells to avoid immune cell activity in vivo. This study emphasizes the potential of this approach as a therapy for carcinoembryonic antigen-expressing primary @entity14 and its metastases.

Gene therapy of XXXX -derived T lymphocytes to target and eradicate colorectal hepatic metastases.

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity1 :: ('9606', 'Species') :: ['patients', 'Patient'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal disease', 'colorectal tumor', 'colorectal cancer'] @entity12711 :: ('919', 'Gene') :: ['CD3zeta']
@entity1 :: (9606,Species) :: ['patient']
