UNASSIGNED: This study investigated multidrug resistance in Shewanella xiamenensis isolated from an estuarine water sample in China during 2014. This strain displayed resistance or decreased susceptibility to @entity869 , @entity5646 , @entity6944 , @entity3524 , @entity2755 , @entity2177 , @entity2929 , @entity7913 and @entity799 . The antimicrobial resistance genes aacA3, blaOXA-199, qnrA1 and sul1 were identified by PCR amplification and by sequencing. Pulsed-field gel electrophoresis and DNA hybridization experiments showed that the @entity1536 resistance gene qnrA1 was chromosomally located. qnrA1 was located in a complex class 1 integron, downstream from an ISCR1, and bracketed by two copies of qacE  1-sul1 genes. This integron is similar to In825 with four gene cassettes aacA3, catB11c, dfrA1z and aadA2az. An IS26-mel-mph2-IS26 structure was also detected in the flanking sequences, conferring resistance to @entity7187 . This is the first identification of the class 1 integron in S. xiamenensis. This is also the first identification of the qnrA1 gene and IS26-mediated @entity7646 resistance genes in S. xiamenensis. Presence of a variety of resistance genetic determinants in environmental S. xiamenensis suggests the possibility that this species may serve as a potential vehicle of antimicrobial resistance genes in aquatic environments.

Identification of an integron containing the XXXX resistance gene qnrA1 in Shewanella xiamenensis.

@entity7913 :: ('MESH:D007612', 'Chemical') :: ['kanamycin'] @entity799 :: ('MESH:D015662', 'Chemical') :: ['trimethoprim-sulfamethoxazole'] @entity7646 :: ('CHEBI:25106', 'Chemical') :: ['macrolide'] @entity6944 :: ('MESH:C043266', 'Chemical') :: ['cefepime'] @entity2929 :: ('MESH:D004917', 'Chemical') :: ['erythromycin'] @entity2177 :: ('MESH:D002939', 'Chemical') :: ['ciprofloxacin'] @entity3524 :: ('MESH:D002439', 'Chemical') :: ['cefotaxime'] @entity7187 :: ('MESH:D018942', 'Chemical') :: ['macrolides'] @entity869 :: ('MESH:D000667', 'Chemical') :: ['ampicillin'] @entity2755 :: ('MESH:D002701', 'Chemical') :: ['chloramphenicol'] @entity5646 :: ('MESH:D001398', 'Chemical') :: ['aztreonam'] @entity1536 :: ('CHEBI:23765', 'Chemical') :: ['quinolone']
@entity1536 :: (CHEBI:23765,Chemical) :: ['quinolone']
