Sphingomonas sp. strain HV3 (formerly Pseudomonas sp. HV3), which degrades aromatics and chloroaromatics, harbors a mega-plasmid, pSKY4. A sequenced 4 kb fragment of the plasmid reveals a novel gene organization for @entity6153 meta-pathway genes. The putative meta operon starts with the cmpF gene encoding a 2-hydroxymuconic @entity26489 hydrolase. The gene has a 6 bp overlap with the previously characterized ring-cleavage gene, @entity6153 2,3-dioxygenase, cmpE. Downstream of cmpE is a 429 bp open reading frame of unknown function. Gene cmpC, encoding a 2-hydroxymuconic @entity26489 dehydrogenase, starts 44 bp further downstream. It has the highest homology to 2-hydroxymuconic @entity26489 dehydrogenases of dmp and xyl pathways and to XylC from the marine oligotroph Cycloclasticus oligotrophus. The gene organization is different from other known meta pathways. This is the first report of organization of plasmid-encoded meta-pathway genes in the genus Sphingomonas.

Novel organization of XXXX meta-pathway genes in Sphingomonas sp. HV3 pSKY4 plasmid.

@entity26489 :: ('CHEBI:26643', 'Chemical') :: ['semialdehyde'] @entity6153 :: ('MESH:C034221', 'Chemical') :: ['catechol']
@entity6153 :: (MESH:C034221,Chemical) :: ['catechol']
