The two-electrode voltage-clamp technique was used to evaluate the effect of protein kinase C (PKC) activation on ion current flow in @entity1238 oocytes injected with cRNA coding for the @entity1 vanilloid receptor ( @entity13812 ). In the presence of 30 nM @entity10670 ( @entity10671 ), current evoked by an effective concentration (EC(30)) of @entity2951 (CAP) was potentiated by 638+/-117% (n=8). PDBu exhibited an EC(50) of about 17+/-3 nM for this effect (n=8). Potentiation was not observed when @entity13812 expressing oocytes were exposed to both 30 nM PDBu and 1 microM @entity866 . In the presence of 300 nM PDBu, the EC(50) for CAP shifted from 899+/-78 to 139+/-2 1 nM (n=11 and 5, respectively). In the presence of 30 nM PDBu, the maximal current amplitude evoked by application of CAP increased by 86+/-21% (n=10), in a @entity866 sensitive manner. Application of 1 microM PDBu alone elicited a @entity4413 sensitive current within 3 min of exposure. This effect was observed in the absence of previous exposure of the oocyte to CAP and was abolished in the presence of 1 microM @entity866 . No current was elicited during a 10 min application of 300 nM PDBu, the longest interval assessed. Prior to 30 nM PDBu exposure, no current was evoked at temperature ramps from room temperature (22-23 degrees C) up to 37 degrees C at pH 6.8, 7.0, or 7.4. Following PDBu treatment, @entity13812 mediated current was evoked at 26 degrees C at pH 7.0. Likewise, following 30 nM PDBu treatment, current was evoked by application of pH 6.8 alone and a further increase in current amplitude was evoked by heat at 24 degrees C in a @entity866 sensitive manner. These data provide direct evidence that PKC activation can increase @entity13812 current evoked by candidate physiological activators, pH and heat. This observation provides an empirical foundation for explaining some types of @entity158 in terms of PKC activation, small decreases in tissue pH levels, and small increases in skin temperature, all of which can accompany inflammatory conditions.

Activation of protein kinase C sensitizes @entity1 XXXX to @entity2951 and to moderate decreases in pH at physiological temperatures in @entity1238 oocytes.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1238 :: ('8355', 'Species') :: ['Xenopus laevis'] @entity2951 :: ('MESH:D002211', 'Chemical') :: ['capsaicin'] @entity4413 :: ('MESH:C071423', 'Chemical') :: ['capsazepine'] @entity10670 :: ('MESH:D015240', 'Chemical') :: ['phorbol-12,13-dibutyrate'] @entity866 :: ('MESH:D019311', 'Chemical') :: ['staurosporine'] @entity158 :: ('MESH:D010146', 'Disease') :: ['inflammatory pain'] @entity10671 :: ('CHEBI:17598', 'Chemical') :: ['PDBu'] @entity13812 :: ('7442', 'Gene') :: ['VR1']
@entity13812 :: (7442,Gene) :: ['VR1']
