BACKGROUND: @entity519 inducible factor-1a ( @entity1965 ) is the central transcriptional regulator of @entity519 responses during the progression of @entity977 . Although previous immunohistochemical studies revealed that @entity1965 is expressed in adreno-cortico-tropic-hormone (ACTH) @entity977 , the role of @entity1965 remains unclear. METHODS: AtT-20 cells were incubated under @entity519 conditions (1% O2) for 12 h. @entity1965 mRNA and protein expression levels were measured by real-time PCR and western blotting, respectively. BrdU was used to determine the effects of @entity519 on cell viability. AtT-20 cells were transfected with siRNA targeting @entity1965 , followed by @entity519 (1% O2) for 12 h. Apoptosis was determined by @entity10440 -FITC flow cytometry and Tdt-mediated dUTP nick end-labelling (TUNEL) assay. In addition, we examined interactions between @entity1965 , glucocorticoid receptor (GR), and dexamethasone under both normoxic and @entity519 conditions. RESULTS: @entity519 triggered the time-dependent proliferation of AtT-20 cells in association with increased @entity1965 mRNA and protein levels. However, the viability of AtT-20 cells decreased greatly when they were first transfected with @entity1965 -siRNA and then exposed to @entity519 . According to flow cytometry ( @entity10440 -FITC and PI staining) and TUNEL analyses, a greater percentage of cells were apoptotic when transfected with @entity1965 siRNA and subsequently cultured under @entity519 conditions compared to those in the normoxia and mock groups. After AtT-20 cells were cultured in 1% O2 and then treated with dexamethasone, @entity1965 levels significantly increased or decreased in normoxic or @entity519 conditions, respectively. Dexamethasone suppressed GR expression to a higher degree in @entity519 than normoxic conditions. Downregulation of GR by dexamethasone was greatly prevented in cells that were transfected with @entity1965 siRNA. CONCLUSIONS: These findings strongly suggest that @entity1965 exerts an antiapoptotic role and participates in the downregulation of GR by dexamethasone in @entity519 AtT-20 cells.

Effects of XXXX inducible factor-1a on apoptotic inhibition and glucocorticoid receptor downregulation by dexamethasone in AtT-20 cells.

@entity10440 :: ('11747(Tax:10090)', 'Gene') :: ['annexin V'] @entity519 :: ('MESH:D000860', 'Disease') :: ['Hypoxia', 'hypoxic', 'hypoxia'] @entity977 :: ('MESH:D010911', 'Disease') :: ['pituitary adenomas'] @entity1965 :: ('15251(Tax:10090)', 'Gene') :: ['HIF-1a']
@entity519 :: (MESH:D000860,Disease) :: ['hypoxia']
