BACKGROUND: It has been suggested that in the granules of @entity35 mast cells there is some kind of @entity224 dismutase (SOD), but details of this SOD in mast cells remain unclear. In the present study, we studied the mode of existence of SOD in mast cells and its releasing mechanism from the granules. In addition, we discussed the physiological role of SOD in allergic events. METHODS: Purified @entity35 mast cells were disrupted with a sonic disrupter and granules (sample I) were separated from supernatant (sample II) by centrifugation. The granules were treated with 1 mM @entity141 , and the supernatant (sample III) was separated from the pellet (sample IV). Sample III was applied to a heparin column and the eluate was used as sample V. SOD activity was measured in these samples. RESULTS: SOD existed in mast cell granules as a heparin-binding and an inactive form. However, when granules were released and exposed to high @entity141 concentration, SOD was discharged from heparin and shifted to the active form. The expression of @entity9269 mRNA was enhanced when @entity273 ( @entity274 ) or sample III with the @entity3624 - @entity3624 oxidase system were added to the culture media. CONCLUSIONS: These findings suggest that in stimulated @entity35 mast cells, the released SOD may transform the generated @entity224 anion into @entity274 , and the generated @entity274 may enhance the expression of chemokine mRNA in the mast cells.

Roles of @entity224 dismutase in XXXX mast cell granules.

@entity9269 :: ('25542(Tax:10116)', 'Gene') :: ['macrophage inflammatory protein-1 alpha'] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H(2)O(2)'] @entity3624 :: ('MESH:D019820', 'Chemical') :: ['xanthine'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['hydrogen peroxide'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity224 :: ('MESH:D013481', 'Chemical') :: ['superoxide']
@entity35 :: (10116,Species) :: ['rat']
