BACKGROUND: Nerve growth factor (NGF) is known not only as a major factor for neuronal plasticity but also as a @entity158 stimulator. Although there have been several trials with NGF for its application in the regeneration or protection of the nervous system, the @entity158 induced by NGF remains a challenge to be overcome. In this study, the @entity158 induced by NGF gene therapy was evaluated. RESULTS: Vehicle or recombinant @entity235 NGF plasmid was administered into the intrathecal space of @entity235 . Twenty-four hours after the vehicle or NGF plasmid inoculation, @entity235 were subcutaneously treated with 150  mg/kg @entity2764 every day for 7  days. For @entity158 assessment, physical examination and electrophysiological recording were performed. Only in the vehicle-treated group, @entity353 occurred, while NGF plasmid inoculation significantly improved this physical abnormalities. In the vehicle-treated group, electrophysiological recordings showed that @entity8108 -reflex disappeared at 24  @entity8108 after the last @entity2764 treatment. However, in the NGF plasmid inoculated group, the @entity8108 -reflex were normal. In the results of immunohistochemistry, the NGF plasmid administration efficiently expressed in the dorsal root ganglia and significantly increased the @entity2764 -induced reduction of calcitonin gene-related peptide (CGRP) immunoreactive neurons, but not in substance P immunoreactive neurons, in the dorsal root ganglia. CONCLUSIONS: Given these results, we reason that NGF gene therapy in @entity2764 induced @entity1109 @entity235 does not induce @entity9 with this dosage, even with increasing the expression of CGRP.

SP, CGRP changes in @entity2764 induced @entity1109 XXXX with nerve growth factor gene therapy.

@entity2764 :: ('MESH:D011736', 'Chemical') :: ['pyridoxine'] @entity353 :: ('MESH:D015431', 'Disease') :: ['weight loss'] @entity235 :: ('9615', 'Species') :: ['dog', 'dogs'] @entity1109 :: ('MESH:D012678', 'Disease') :: ['neuropathic'] @entity158 :: ('MESH:D010146', 'Disease') :: ['pain'] @entity8108 :: ('D006859', 'Chemical') :: ['h', 'H'] @entity9 :: ('MESH:D009437', 'Disease') :: ['neuropathic pain']
@entity235 :: (9615,Species) :: ['dogs']
