T cells play a major part in the immune response in @entity2520 . In order to determine any selective T-cell receptor (TCR) beta-chain variable region (V beta) usage in the infiltrates of healthy/ @entity2522 (H/G) and @entity7278 ( @entity7278 ), cells were extracted from gingival biopsies, the CD4 and CD8 cells stained with antibodies to eight V beta regions, and two-colour flow cytometry used to analyse the data. The frequencies of CD4 and CD8 cells expressing each of the TCR-V beta families varied from 0 to 46% between individuals. A high percentage of CD4 and CD8 cells expressed the V beta 13 family in several @entity7278 biopsies, but, in a number of H/G tissues, a high percentage of T cells expressed up to three families including the V beta 13 region, these varying from individual to individual. The mean results showed a significantly greater percentage of V beta 5.2-3-positive CD4 cells (p = 0.003) and V beta 5.1- and 5.2-3-positive CD8 cells (p = 0.003 and 0.025, respectively) isolated from H/G than @entity7278 tissues. The percentage of V beta 3.1-positive CD4 cells extracted from H/G tissues was also higher but not quite significant at the 0.05 level (p = 0.051). Sections of gingival tissue in biopsies from H/G and @entity7278 were stained in situ; there were no significant differences in the mean expression of V beta 3.1-, 5.1- or 5.2-3-positive cells. A second aim was to determine the effect of @entity2523 on the TCR repertoire. There were no differences in the mean percentage of CD4 or CD8 cells expressing the eight TCR-V beta regions between the two groups after stimulation in vitro with @entity2523 outer-membrane antigens. There was, however, a trend towards a decrease in the percentage of positive CD4 and CD8 T cells after culture with the antigen. This was significant for CD4 cells from H/G expressing the V beta 5.1 and 5.3 TCRs (p = 0.032 and p = 0.038, respectively). This trend was not evident for V beta 5.2-3-positive CD4 cells or V beta 5.1-positive CD8 cells isolated from both H/G and @entity7278 nor for V beta 3.1-positive CD8 cells from @entity7278 . The results show that there may be restricted V beta usage in gingival tissues, particularly in H/G tissues. The V beta 5 and 3.1 families may be selected for in the gingival tissues and may also be involved in @entity2523 activation.

Expression of receptor beta-chain variable region by T cells in human XXXX .

@entity2523 :: ('837', 'Species') :: ['Porphyromonas gingivalis', 'P. gingivalis'] @entity2522 :: ('MESH:D005891', 'Disease') :: ['gingivitis'] @entity2520 :: ('MESH:D010510', 'Disease') :: ['periodontal diseases'] @entity7278 :: ('MESH:D055113', 'Disease') :: ['adult periodontitis', 'AP']
@entity2520 :: (MESH:D010510,Disease) :: ['periodontal disease']
