The aims of this study were to ascertain whether @entity7292 ( @entity4673 ) concentrations below those used in clinical practice inhibit bacterial growth, and in parallel study the cytotoxic effects on @entity1 fibroblasts. The cytoprotective effects of fetal calf serum (FCS) were also evaluated. The cytotoxic effects of eight different @entity7292 concentrations were determined by assessing mitochondrial activity of viable cells capable of cleaving @entity34175 . Antimicrobial activity of @entity4673 , range: 7-550 x 10(-5)%, was tested against @entity794 , @entity8171 , and @entity796 . @entity7292 concentrations exerting antimicrobial effects were: @entity794 , >70 x 10(-5)%; @entity796 , >/=270 x 10(-5)%, and @entity8171 , >/=550 x 10(-5)%. With 2% FCS, the lowest @entity4673 concentration studied (7 x 10(-5)%) showed cytotoxic effects (cell survival 71 +/- 19%) at only 2 h of incubation. Under these conditions @entity4673 @entity137 was time- and concentration-dependent in all exposure periods. @entity137 was greatly enhanced causing 76% fibroblast growth inhibition at concentrations of 14 x 10(-5)% and contact time of 2 h. The @entity4673 concentration of 7 x 10(-5)% was also cytotoxic with 5% FCS in the media compared with controls, although cell survival was higher than with 2% FCS. The cytoprotective action of FCS was clearly shown at the concentration of 10% at which @entity4673 @entity137 of 7 x 10(-5)% to 28 x 10(-5)% was partially or completely inhibited. Our results show that @entity4673 at concentrations 100-700 times more diluted than that normally used in clinical practice retained effective inhibitory activity against some of the above-mentioned microorganisms. However, even these concentrations are cytotoxic for cultured fibroblasts. Thus, @entity7292 concentrations up to 100 times more diluted can be used, since they possess bacterial growth-inhibiting power, are less cytotoxic and therefore favour wound healing.

@entity7292 : antimicrobial activity related to @entity137 in cultured XXXX fibroblasts.

@entity1 :: ('9606', 'Species') :: ['human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['Cytotoxicity', 'cytotoxicity'] @entity796 :: ('287', 'Species') :: ['Pseudomonas aeruginosa', 'P. aeruginosa'] @entity7292 :: ('MESH:D012835', 'Chemical') :: ['Silver nitrate', 'silver nitrate'] @entity794 :: ('1280', 'Species') :: ['Staphylococcus aureus', 'S. aureus'] @entity8171 :: ('546', 'Species') :: ['C. freundii', 'Citrobacter freundii'] @entity4673 :: ('CHEBI:32130', 'Chemical') :: ['AgNO3'] @entity34175 :: ('MESH:D013778', 'Chemical') :: ['tetrazolium salts']
@entity1 :: (9606,Species) :: ['human']
