BACKGROUND: Preclinical evaluations of chemotherapies depend on clinically relevant animal models for @entity788 . The injection of syngeneic @entity19 @entity420 cells is one efficient option to generate @entity189 in @entity19 with an intact immune system. However, this option is constrained by the paucity of appropriate cell lines. RESULTS: The @entity19 pancreatic @entity420 cell lines 6606PDA and 7265PDA were compared to the 6606l cell line isolated from a liver @entity3 from @entity19 suffering from @entity788 . In tissue culture 6606PDA and 6606l proliferated faster than 7265PDA. 7265PDA cells were, however, significantly more sensitive to @entity136 as assessed by @entity1603 -incorporation and @entity3460 exclusion assays in vitro. Within 1  week after injection of either one of these three cell lines into the pancreas of C57BL/6J @entity19 , @entity189 were observed by T2 weighted magnetic resonance imaging and histology. Three weeks after injecting 6606PDA or 6606l cells large @entity189 could be characterized, which were surrounded by extensive @entity2269 . After injection of 7265PDA cells, however, remission of @entity5 was observed between the first and the third week. Compared to 6606PDA cell derived @entity189 a higher apparent diffusion coefficient was quantified by diffusion weighted magnetic resonance imaging in these @entity5 . This correlated with reduced @entity5 cell density observed on histological sections. CONCLUSION: All three cell lines can be used in vitro for testing combinatorial therapies with @entity136 . The 6606PDA and 6606l cell lines but not the 7265PDA cell line can be used for evaluating distinct therapies in a @entity189 model using C57BL/6J @entity19 . Diffusion-weighted MRI proved to be an appropriate method to predict @entity5 remission.

Characterization of novel @entity189 cell lines for the analysis of therapeutical strategies fighting XXXX .

@entity1603 :: ('MESH:D001973', 'Chemical') :: ['BrdU'] @entity788 :: ('MESH:D010190', 'Disease') :: ['pancreatic cancer'] @entity19 :: ('10090', 'Species') :: ['mice', 'murine'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'tumors'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinomas', 'syngeneic carcinoma'] @entity2269 :: ('MESH:D058405', 'Disease') :: ['desmoplastic reaction'] @entity136 :: ('MESH:C056507', 'Chemical') :: ['gemcitabine'] @entity3460 :: ('MESH:D014343', 'Chemical') :: ['trypan blue'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity420 :: ('MESH:D000230', 'Disease') :: ['adenocarcinoma']
@entity788 :: (MESH:D010190,Disease) :: ['pancreatic cancer']
