Latent transforming growth factor (TGF)-beta-binding protein (LTBP) is required for the assembly, secretion, matrix association, and activation of latent TGF-beta complex. To elucidate the cell specific expression of the genes of @entity2535 and their splice variants and the factors that regulate the gene expression, we cultured primary @entity1 glomerular endothelial cells (HGEC) under different conditions. Basal expression of @entity2535 mRNA was suppressed in HGEC compared to WI-38 @entity1 embryonic lung fibroblasts. High @entity413 , @entity274 , and @entity2535 upregulated and @entity7 ( @entity7 ) further downregulated @entity2535 mRNA in HGEC. RT-PCR with a primer set for LTBP-1S produced many clones but no clone was gained with a primer set for LTBP-1L. Of 12 clones selected randomly, Sca I mapping and DNA sequencing revealed that only one was LTBP-1S and all the others were LTBP-1Sdelta53. @entity2535 , but not high @entity413 , @entity274 or @entity7 , tended to increase LTBP-1Sdelta53 mRNA. In conclusion, HGEC express @entity2535 mRNA which is suppressed at basal state but upregulated by high @entity413 , @entity274 , and @entity2535 and downregulated by @entity7 . Major splice variant of @entity2535 in HGEC was LTBP-1S 53. Modification of LTBP-1S 53 gene in HGEC may abrogate fibrotic action of @entity2535 but this requires confirmation.

Expression and regulation of latent TGF-beta binding protein-1 transcripts and their splice variants in XXXX glomerular endothelial cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H(2)O(2)'] @entity2535 :: ('4052', 'Gene') :: ['LTBP-1', 'TGF-beta1'] @entity7 :: ('7422', 'Gene') :: ['vascular endothelial growth factor', 'VEGF'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity1 :: (9606,Species) :: ['human']
