The presence of four different isoforms of luteinizing hormone-releasing hormones (LHRH) and one LHRH receptor ( @entity23042 ) has been reported in vertebrates. In the @entity1 genome only LHRH-I and @entity36209 genes have been identified. The @entity1 LHRH-I gene is composed of four exons separated by three introns. Three LHRH receptor or receptor-like genes have been demonstrated. The well-established type-I LHRH receptor ( @entity23042 -I) gene is composed of three exons separated by two introns. In this study we investigated the expression of transcript forms of @entity23042 -I in @entity1 @entity1067 ( @entity1067 ) with reverse transcriptase-polymerase chain reaction (RT-PCR) using gene specific primers. Thirty-five @entity1 @entity1067 specimens were obtained at surgery. Normal @entity1 pituitaries collected at autopsy served as control. RNA extraction and RT-PCR with gene-specific primers for @entity23042 -I forward (F1)/reverse (R1), @entity23042 -I F2/R3, @entity23042 -I F1'/R2' were carried out to determine the mRNA expression for @entity23042 -I transcript forms. The expected PCR products amplified with gene specific primers were @entity4960 with 319 bp, @entity23042 -I F2/R3 with 309 bp and @entity23042 -I F1'/R2' with 219 bp. PCR products for @entity4960 were detected in 21 (60%) and for @entity23042 -I F2/R3 in 5 of 35 (14%) @entity1067 samples. No PCR products for @entity23042 -I F1'/R2' were found. In conclusion, we detected mRNA for @entity23042 -I in @entity1 @entity1067 specimens. Our results suggest that @entity23042 -I gene may have more than two splice variants or uncharacterised transcript forms of @entity23042 -I. Our findings support the merit of further investigation of the expression of @entity23042 -I and its transcript forms in @entity1 @entity1067 .

Expression of mRNA for @entity1 type-I LHRH receptor transcript forms in XXXX @entity1067 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity1067 :: ('MESH:D011470', 'Disease') :: ['benign prostatic hyperplasia', 'BPH'] @entity23042 :: ('2798', 'Gene') :: ['LHRH-R'] @entity36209 :: ('2797', 'Gene') :: ['LHRH-II'] @entity4960 :: ('910', 'Gene') :: ['LHRH-R-I F1/R1']
@entity1 :: (9606,Species) :: ['human']
