The @entity1534 iceA gene was recently identified as a genetic marker for the development of peptic @entity1066 in a Western population. To assess the significance of iceA subtypes of @entity1534 in relation to peptic @entity1066 , 140 Japanese clinical isolates (88 from Fukui and 52 from Okinawa) were characterized. Sequence analysis of the iceA1 gene from 25 representative Japanese strains was also carried out to identify the differences in iceA between the @entity1066 group and the @entity3621 group. The iceA1 genotype was not correlated with the presence of peptic ulceration in either area. In addition, sequence analysis led to identification of five deletions and five point mutations (a nonsense mutation or a 1-bp insertion) within the iceA1 open reading frame corresponding to previously published sequences. These mutations were identified in both clinical groups ( @entity1066 and @entity3621 groups) in each area. Local DNA sequence analysis revealed that the endpoints of all five deletions coincided with direct repeats. We also found four strains that carried longer iceA1 open reading frames compared with that for strain 60190. In conclusion, carriage of an iceA1 strain does not seem to be a risk factor for peptic @entity1066 in Japanese subjects. The critical mutations in the iceA1 gene in some isolates from @entity1 with peptic ulcers suggested that IceA does not participate in the pathogenesis of peptic @entity1066 in Japan. We also found deletion hot spots that were associated with direct repeats in iceA1 and that favored a small-deletion model of slipped mispairing events during replication. We showed that iceA1 sequence variations may be useful tools for analysis of the population genetics of @entity1534 .

Sequence analysis and clinical significance of the iceA gene from XXXX strains in Japan.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity1066 :: ('MESH:D014456', 'Disease') :: ['ulcer'] @entity3621 :: ('MESH:D005756', 'Disease') :: ['gastritis'] @entity1534 :: ('210', 'Species') :: ['H. pylori', 'Helicobacter pylori']
@entity1534 :: (210,Species) :: ['Helicobacter pylori']
