Deregulated signaling by the epidermal growth factor receptor family of proteins is encountered in @entity1 @entity5 including @entity0 . Cell cycle and apoptosis-regulatory protein-1 ( @entity7136 -1), a novel, perinuclear phosphoprotein, is a regulator of apoptosis signaling by epidermal growth factor receptors. @entity7136 -1 expression is diminished in @entity1 @entity0 , and correlates inversely with @entity1 @entity0 grades which could be attributed to increased methylation. The expression of @entity7136 -1, on the other hand, interferes with the ability of @entity1 @entity0 cells to invade through the matrigel-coated membranes, to form colonies in the soft agar, and to grow as s.c. @entity5 in @entity466 ( @entity466 ) @entity19 . To test whether @entity7136 -1 is a suppressor of @entity1 @entity0 growth, we generated transactivator of transcription (TAT)-tagged @entity7136 -1 peptides. Treatment of @entity1 @entity0 cells with affinity purified, TAT- @entity7136 -1 1-198, 197-454, and 896-1150 peptides caused inhibition of @entity1 @entity0 cell proliferation and elevated apoptosis. In contrast, TAT-tagged enhanced green fluorescent protein or @entity7136 -1 (1-198( @entity70247 )) peptide failed to inhibit cell proliferation or induce apoptosis. Apoptosis by @entity7136 -1 peptides, with the exception of @entity7136 -1 (1-198( @entity70247 )), involves the activation of p38 stress-activated protein kinase and caspase-9. Moreover, administration of TAT- @entity7136 -1 (1-198), but not TAT-tagged enhanced green fluorescent protein or TAT- @entity7136 -1 (1-198( @entity70247 )), inhibits growth of @entity1 @entity0 cell-derived @entity5 xenografts in @entity466 @entity19 . We conclude that @entity7136 -1 is a suppressor of @entity1 @entity0 growth, and its expression is diminished in @entity5 , in part, by methylation-dependent silencing.

Transactivator of transcription-tagged cell cycle and apoptosis regulatory protein-1 peptides suppress the growth of @entity1 XXXX cells in vitro and in vivo.

@entity1 :: ('9606', 'Species') :: ['human'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancers', 'breast cancer'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors', 'malignancies'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity70247 :: ('p|SUB|Y|192|F', 'ProteinMutation') :: ['Y192/F'] @entity7136 :: ('7962', 'Species') :: ['CARP'] @entity466 :: ('MESH:D016511', 'Disease') :: ['SCID', 'severe combined immunodeficiency']
@entity0 :: (MESH:D001943,Disease) :: ['breast cancer']
