PURPOSE: After @entity1566 ( @entity1566 ) a @entity66 is generated in the area affected that forms a barrier for axon growth and myelination, preventing functional recovery. Recently, we have described a synthetic glycolipid ( @entity44774 ) that inhibited proliferation of @entity1 @entity11 cells. We show now that @entity44774 inhibited the proliferation of astrocytes and microglial cells, the principal cellular components of the @entity66 , and promoting axonal outgrowth and myelin production in vitro. METHODS: Glial cells were inhibited with @entity44774 (IC50   10  M) and studied by RT-PCR, Western Blotting, immunoprecipitation and fluorescence microscopy. Axonal outgrowth in dorsal root ganglia (DRG) and myelin production by oligodendrocytes were analyzed by immunocytochemistry. Adult @entity35 were assayed in @entity1566 model and the recovery of treated animals (n=6) and controls (n=6) was followed. RESULTS: The @entity44774 was localized in the cytosol of glial cells, forming a complex with RhoGDIa, a regulator of RhoGTPases. Treatment of astroglial cultures with @entity44774 increase the expression of BDNF receptor genes (TrkBT1, @entity12715 Full). @entity44774 reduced the astroglial marker GFAP, while increasing production of @entity2610 in oligodendrocytes and promoted axonal outgrowth from DRG neurons. Local injection of @entity44774 , near a @entity1566 , promoted the recovery of lesioned animals analyzed by BBB test (P< 0.05). CONCLUSIONS: We propose that inhibition of astrocytes and microglia by @entity44774 could be diminished the @entity66 formation, inducing the re-growth and myelination of axons, these elements constitute a new approach for @entity1566 therapy.

Inhibition of glial proliferation, promotion of axonal growth and myelin production by synthetic glycolipid: A new approach for XXXX treatment.

@entity1 :: ('9606', 'Species') :: ['human'] @entity2610 :: ('4155', 'Gene') :: ['myelin basic protein'] @entity66 :: ('MESH:D004194', 'Disease') :: ['glial scar'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity44774 :: ('8567', 'Gene') :: ['IG20'] @entity11 :: ('MESH:D005910', 'Disease') :: ['glioma'] @entity1566 :: ('MESH:D013119', 'Disease') :: ['spinal cord injury', 'SCI', 'spinal cord contusion'] @entity12715 :: ('4915', 'Gene') :: ['TrkB']
@entity1566 :: (MESH:D013119,Disease) :: ['spinal cord injury']
