RATIONALE: @entity25218 ( @entity25218 ) is a low molecular weight drug of the @entity3096 group, which has an anti-vascular effect in @entity5 causing endothelial cell apoptosis and activation of cytokines. @entity3096 -based compounds have been reported to lead to an upregulation in the expression of lysophosphatidylcholines (LPC)-type lipids in solid @entity5 . A study employing TLC/MALDI-MS and MALDI-MS imaging to examine LS174T @entity14 xenografts following administration of @entity25218 has been conducted into this effect. METHODS: LS174T @entity14 xenografts grown in male immune-deficient @entity19 were treated with 27.5  mg/kg @entity25218 . The control (before treatment) and 4  h and 24  h post-treatment @entity5 were excised and divided into two. MALDI-MS imaging experiments were carried out on 12    m cryosections sections taken from one half of the @entity5 and from the other half the lipids were extracted and analysed by TLC/MALDI-MS. These experiments were carried out in triplicate. RESULTS: Statistical analysis of the MALDI-MS imaging data set indicated an increased amount of LPC in the 24  h post-treated sample and a decreased amount of PC in the 24  h post-treated sample, compared with the 4  h post-treated sample and the control. These effects were confirmed by the TLC/MALDI-MS data. The lipid extracts were separated into six spots on the TLC plate. These were identified as arising from different lipids classes, i.e. LPC, @entity19846 (SM), @entity2434 (PC) and @entity25579 (PE). The TLC/MALDI-MS data indicated that LPC were highly expressed in the 4  h and 24  h post-treated @entity5 samples compared with the control. Examination of the mass spectrometric images confirms this increase and demonstrates additionally that the increase in the signals arising from LPC appears to be localised primarily within the central areas of the xenograft. CONCLUSIONS: An increase in expression of LPC lipids in solid @entity5 treated with @entity25218 has been demonstrated and shown to be localised in the central area of the @entity5 . Copyright    2015 John Wiley _ Sons, Ltd.

Thin-layer chromatography/matrix-assisted laser desorption/ionisation mass spectrometry and matrix-assisted laser desorption/ionisation mass spectrometry imaging for the analysis of phospholipids in LS174T XXXX xenografts treated with the vascular disrupting agent @entity25218 .

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumour', 'tumours'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity25579 :: ('MESH:D010714', 'Chemical') :: ['phosphatidylethanolamines'] @entity25218 :: ('MESH:C066668', 'Chemical') :: ['5,6-Dimethylxanthenone-4-acetic acid', 'DMXAA'] @entity2434 :: ('MESH:D010713', 'Chemical') :: ['phosphatidylcholines'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal adenocarcinoma'] @entity19846 :: ('MESH:D013109', 'Chemical') :: ['sphingomyelins'] @entity3096 :: ('CHEBI:47916', 'Chemical') :: ['Flavonoid', 'flavonoid']
@entity14 :: (MESH:D015179,Disease) :: ['colorectal adenocarcinoma']
