@entity1 Cripto-1 ( @entity43746 ) is a cell membrane protein that is overexpressed in several different types of @entity1 @entity189 . In the present study we investigated the mechanisms that regulate the expression of @entity43746 gene in @entity5 cells. We cloned a 2,481 bp 5'-flanking region of the @entity1 @entity43746 gene into a luciferase reporter vector and transfected NTERA-2 @entity1 @entity189 cells and LS174-T @entity694 cells to test for promoter activity. Activity of @entity43746 promoter in both cell lines was modulated by two TGF-beta family members, @entity2533 and @entity13062 . In particular, @entity2533 significantly up-regulated @entity43746 promoter activity, whereas a dramatic reduction in @entity43746 promoter activity was observed with @entity13062 in NTERA-2 and LS174-T cells. Changes in the @entity43746 promoter activity following @entity2533 and @entity13062 treatments correlated with changes in @entity43746 mRNA and protein expression in NTERA-2 and LS174-T cells. We also identified three Smad binding elements (SBEs) within the @entity43746 promoter and point mutation of SBE1 (-2,197/-2,189) significantly reduced response of the @entity43746 promoter to both @entity2533 and @entity13062 in NTERA-2 and LS174-T cells. Chromatin immunoprecipitation assay also demonstrated binding of @entity1594 to a @entity43746 promoter DNA sequence containing SBE1 in LS174-T cells. Finally, @entity13062 inhibited migration of LS174-T cells and F9 @entity19 @entity189 cells by downregulation of @entity43746 protein. In conclusion, these results suggest a differential modulation of @entity43746 gene expression in embryonal and @entity694 cells by two different members of the TGF-beta family.

Regulation of @entity1 Cripto-1 gene expression by @entity2533 and @entity13062 in embryonal and XXXX cells.

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity13062 :: ('652', 'Gene') :: ['BMP-4'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinomas', 'embryonal carcinoma'] @entity2533 :: ('7040', 'Gene') :: ['TGF-beta1'] @entity1594 :: ('4089', 'Gene') :: ['Smad-4'] @entity43746 :: ('21667(Tax:10090)', 'Gene') :: ['CR-1'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon cancer']
@entity694 :: (MESH:D003110,Disease) :: ['colon cancer']
