The aim of this study was to investigate whether synaptic plasticity and metaplasticity in slice cultures of the young @entity35 hippocampus were comparable to previously reported synaptic plasticity and metaplasticity in acute adult hippocampal slices. This is relevant since differences do exist between the preparations as a result of age and the ex vivo maintenance. We prepared and maintained slice cultures from 5- to 6-day-old @entity35 according to the porous membrane method. After 12-16 days in vitro, extracellular @entity4365 ( @entity4365 ) and @entity2198 ( @entity2198 ) protocols were applied to the Schaffer collaterals, and extracellular field potentials were recorded in area @entity1249 . @entity4365 and @entity2198 induced long-term @entity308 (LTD) and long-term potentiation (LTP), respectively. LTP could be reversed by @entity4365 , as could LTD by @entity2198 60 min after induction. Plotting the amount of LTD and LTP versus stimulation protocol demonstrated frequency-dependence of the sign and extent of plasticity. Priming activation of group 1 metabotropic @entity2227 receptors (mGluRs) with @entity11724 facilitated subsequent LTP, revealing a metaplastic effect similar to that observed in acute slices. Immunohistochemistry for group 1 mGluR subtypes mGluR1alpha and @entity10092 showed both receptors to be present in these cultures. We conclude that synaptic plasticity and mGluR-mediated metaplasticity are largely comparable to those effects found in acute in vitro techniques.

Properties of long-term synaptic plasticity and metaplasticity in organotypic slice cultures of XXXX hippocampus.

@entity2198 :: ('MESH:D006316', 'Disease') :: ['high-frequency stimulation', 'HFS'] @entity308 :: ('MESH:D003866', 'Disease') :: ['depression'] @entity2227 :: ('CHEBI:29987', 'Chemical') :: ['glutamate'] @entity1249 :: ('310218(Tax:10116)', 'Gene') :: ['CA1'] @entity10092 :: ('14805(Tax:10090)', 'Gene') :: ['mGluR5'] @entity11724 :: ('MESH:C010117', 'Chemical') :: ['DHPG'] @entity4365 :: ('MESH:C565121', 'Disease') :: ['LFS', 'low-frequency stimulation'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats']
@entity35 :: (10116,Species) :: ['rat']
