INTRODUCTION: Aptamers are oligonucleotides that have high affinity and specificity for their molecular targets which are emerging as a new class of molecules for radiopharmaceuticals development. In this study, aptamers selected to @entity794 were evaluated for @entity2348 identification. METHODS: Anti S. aureus aptamers were labeled with (99m)Tc by the direct method. The radiolabel yield and complex stability were assessed by thin-layer chromatography (TLC). Three groups of Swiss @entity19 containing 6 animals each were used. The first group was infected intramuscularly in the right thigh with @entity794 . The second group was infected in the same way with @entity5132 and the third group was injected with zymosan to induce @entity32 . After 24h, radiolabeled aptamers (22.2MBq) were injected by the tail vein. The @entity19 were euthanized 4h post injection and tissue sample activities measured in a gamma counter. RESULTS: The (99m)Tc labeled aptamers were stable in saline, plasma and cystein excess. Radiolabeled aptamers showed increased uptake in the kidneys for all groups indicating a main @entity8 , which is consistent with the hydrophilic nature and small size of aptamers. The radiopharmaceutical showed rapid blood clearance indicated by a reduced dose (% ID/g) in the blood. The biodistribution showed that aptamers were able to identify the @entity281 foci caused by @entity794 displaying a target/non-target ratio of 4.0  0.5. This ratio for @entity19 infected with @entity5132 was 2.0  0.4 while for @entity19 with @entity32 was 1.2  0.2. Histology confirmed the presence of @entity281 in groups 1 and 2, and @entity32 in group 3. CONCLUSIONS: The biodistibution study demonstrated a statistically higher uptake in the @entity794 foci relative to @entity32 and @entity5132 infected areas. These results highlight the potential of aptamers labeled directly with (99m)Tc for @entity2348 diagnosis by scintigraphy.

Identification of XXXX @entity281 by aptamers directly radiolabeled with technetium-99m.

@entity32 :: ('MESH:D007249', 'Disease') :: ['aseptic inflammation', 'inflammation'] @entity8 :: ('MESH:D007674', 'Disease') :: ['renal excretion'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity2348 :: ('MESH:D001424', 'Disease') :: ['bacterial infection'] @entity794 :: ('1280', 'Species') :: ['Staphylococcus aureus', 'S. aureus'] @entity5132 :: ('5476', 'Species') :: ['C. albicans'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection']
@entity794 :: (1280,Species) :: ['Staphylococcus aureus']
