UNASSIGNED: Hypermethylation of the promoter region of @entity5 suppressor genes is associated with @entity105 in @entity2 ( @entity2 ). Endobronchial ultrasound with needle aspiration (EBUS-NA) is a semi-invasive method for obtaining cell blocks from lymph nodes, which can be used for epigenetic analyses. To establish the relationship between methylation status of @entity986 , @entity2529 , @entity15893 , @entity1412 and @entity20197 genes in lymph nodes sampled by EBUS-NA, @entity5 staging and prognosis. Methylation status of @entity2529 , @entity986 , @entity15893 , @entity1412 and @entity20197 genes was assessed in EBUS-NA cell blocks from @entity2 @entity1 and related to stage and survival. Eighty-five consecutive @entity1 [mean age 67 (SD 8)] were included. Methylation of >= 1 gene was found in 43 malignant nodes (67  %). A higher prevalence of @entity15893 methylation was observed in @entity151 @entity1 [9/10 (90  %) vs. 15/53 (28  %); p  <  0.001   (2) test]. Methylation of @entity1412 and/or @entity986 was related to advanced staging in @entity1149 ( @entity1149 ) [15/29 (52  %) vs. 6/24 (25  %), p  =  0.048,   (2) test]. @entity1 with @entity1149 showing methylation of @entity1412 and/or @entity986 had also lower 6-month survival (p  =  0.019, log rank test), which persisted after adjustment for age and subtyping (HR  =  6, 95  % CI [1.8-19.5], p  =  0.003, Cox regression). Epigenetic analyses are feasible in EBUS-NA cell blocks and may identify methylation patterns associated with worse prognosis. Methylation of @entity986 and @entity1412 genes in @entity1149 @entity1 was associated with advanced staging and lower 6-month survival.

Assessment of methylation status of locoregional lymph nodes in XXXX using EBUS-NA.

@entity2529 :: ('1612', 'Gene') :: ['DAPK'] @entity1 :: ('9606', 'Species') :: ['Patients', 'patients'] @entity986 :: ('1029', 'Gene') :: ['p16'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung cancer', 'LC'] @entity1149 :: ('MESH:D002289', 'Disease') :: ['NSCLC', 'non-small cell lung cancer'] @entity15893 :: ('11186', 'Gene') :: ['RASSF1a'] @entity20197 :: ('1012', 'Gene') :: ['CDH13'] @entity151 :: ('MESH:D055752', 'Disease') :: ['small cell lung cancer'] @entity1412 :: ('MESH:D011125', 'Disease') :: ['APC'] @entity105 :: ('MESH:D063646', 'Disease') :: ['carcinogenesis']
@entity2 :: (MESH:D008175,Disease) :: ['lung cancer']
