UNASSIGNED: This study tested the hypothesis that @entity851 in @entity4576 is associated with expression of the genes that code for uncoupling proteins (UCP) 2 and 3, members of the mitochondrial channel proteins involved in nutrient sensing and metabolism. The specific objective was to contrast the levels of UCP2 messenger RNA (mRNA) in fast vs slow growing catfish as well as in fed vs fasted catfish. Two distinct UCP2 transcripts were identified and named UCP2a and UCP2b, respectively. @entity377 and @entity535 sequence of catfish UCP2s were highly similar to UCP2 and other UCPs from other fish and mammals (>75%). Expression of UCP2a mRNA was detectable at very low levels in various metabolically active tissues, whereas the expression of UCP2b mRNA was readily detectable in the muscle and heart. In a 21-wk feeding study, fish that grew faster had a greater percent body fat at the end of the study (P < 0.01). Expression of UCP2b mRNA tended to be lower (P < 0.10) in fast growing fish in the middle of the study although levels were similar at the beginning and the end of the study. In the fed vs fasted study, expression of UCP2b mRNA in muscle was increased (P < 0.05) in fish assigned to 30  d of fasting. Our results suggest that, based on the @entity377 and @entity535 sequence similarities and tissue mRNA distribution, catfish UCP2b may be the analog to UCP3. Moreover, our results suggest selection toward growth and associated fat accumulation appears to be independent of muscle UCP2b mRNA expression and UCP2b-mediated mechanisms.

Relationship between expression of muscle-specific uncoupling protein 2 messenger RNA and genetic selection toward growth in XXXX .

@entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity851 :: ('MESH:D006130', 'Disease') :: ['increased growth'] @entity377 :: ('CHEBI:36976', 'Chemical') :: ['nucleotide', 'Nucleotide'] @entity4576 :: ('7998', 'Species') :: ['channel catfish']
@entity4576 :: (7998,Species) :: ['channel catfish']
