We previously reported that HLA-unrestricted CTLs against @entity2355 were induced from @entity694 @entity1 by stimulating peripheral blood lymphocytes (PBLs) with recombinant @entity2355 @entity6621 (rVMUC-1). We have performed adoptive immunotherapy (AI) for two gastric and two @entity694 @entity1 , using the rVMUC-1-stimulated T lymphocytes. A significant level of HLA-unrestricted cytotoxicity against @entity2355 was induced in the two @entity694 @entity1 (pA and pB) during the first adoptive immunotherapy, but extremely reduced during the second AI. During the second stimulation phase, the rate of CD3+CD56+ @entity405 + cells were significantly increased and that of CD3+CD56- @entity404 + cells were significantly decreased in the two @entity694 @entity1 as compared to the first AI. CD3+CD56+ @entity405 + and CD3+CD56- @entity404 + cells were isolated from the second AI of the @entity694 @entity1 (pB) and designated as D856 and D4, respectively. The D4 cells demonstrated a high level of HLA-unrestricted CTL activity against @entity2355 , but D856 cells did not. When D856 cells were mixed with D4 cells at a D856/D4 ratio of 1:3, 1:2, and 1:1 and used as effector cells, the HLA-unrestricted and @entity2355 -specific CTL activity of D4 cells was suppressed in a D856/D4 ratio-dependent manner. Further, D856 cells were highly lytic for the D4 cells demonstrating HLA-unrestricted cytotoxicity against @entity2355 . It is concluded that the reduction in HLA-unrestricted cytotoxicity against @entity2355 during the second AI is attributed to the D856 cells killing @entity2355 -specific CTLs (D4). Thus, the CD3+CD56+ @entity405 + cells seem likely to behave as a suppressor T cell.

CD3+CD56+ XXXX + cells demonstrating a suppressor T cell-like function in the peripheral blood of @entity694 @entity1 .

@entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity404 :: ('920', 'Gene') :: ['CD4'] @entity2355 :: ('4582', 'Gene') :: ['MUC-1'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon cancer'] @entity405 :: ('925', 'Gene') :: ['CD8'] @entity6621 :: ('10245', 'Species') :: ['vaccinia virus']
@entity405 :: (925,Gene) :: ['CD8']
