@entity13880 synthesis after incubation with @entity8454 (ALA) is highly variable from one cell to another within a single cell population and in @entity1 @entity463 in vivo. To improve @entity13880 synthesis, we attempted to modify the @entity13880 synthesis pathway in a C6 @entity11 cell model. To perform this experiment we used confocal microspectrofluorometry to analyse the effects of a highly purified form of @entity69862 ( @entity69862 ) on the kinetics of @entity13880 synthesis after ALA administration to living C6 cells. Our results show that @entity13880 fluorescence was maximal (seven-fold higher than basal values) 3 to 4 hrs. after the beginning of incubation with ALA. @entity69862 depressed this increase in fluorescence nearly to basal levels not only in C6 cells but also in HT29 and HepG2 cells. Fluorescence spectra shape were not affected by @entity69862 , except for intensity. ALA/ @entity13880 -induced photocytoxicity was perfectly correlated with fluorescence intensity recorded in cell cytoplasm. ALA alone (100 microg/ml) did not induce a significant decrease in cell survival, but irradiation of 25 J/cm2 leading to an overall cell death of 60%. @entity69862 added together with ALA suppressed ALA/ @entity13880 -induced @entity6337 . The fact that the @entity69862 -induced decrease in @entity13880 synthesis was not the same in animal and plant cells suggests that the @entity7170 metabolic pathway differs due to the relative amounts of substrate or the effect of inhibitor and that another chemical would be needed alone or in combination with @entity69862 to improve @entity13880 synthesis.

Protoporphyrin IX fluorescence kinetics in C6 @entity463 cells after XXXX incubation: effect of a protoporphyrinogen oxidase inhibitor.

@entity1 :: ('9606', 'Species') :: ['human'] @entity6337 :: ('MESH:D017484', 'Disease') :: ['phototoxicity'] @entity7170 :: ('CHEBI:8337', 'Chemical') :: ['porphyrin'] @entity11 :: ('MESH:D005910', 'Disease') :: ['glioma'] @entity13880 :: ('MESH:C028025', 'Chemical') :: ['PpIX'] @entity8454 :: ('MESH:D000622', 'Chemical') :: ['delta-aminolevulinic acid'] @entity463 :: ('MESH:D005909', 'Disease') :: ['glioblastomas'] @entity69862 :: ('MESH:C475571', 'Chemical') :: ['FP846', 'sulfentrazone']
@entity8454 :: (MESH:D000622,Chemical) :: ['delta-aminolevulinic acid']
