The house dust mite (HDM), Dermatophagoidesfarinae (D. farina), is one of the most important indoor allergen sources and a major elicitor of @entity56 ; itscharacterization is important in the diagnosis and immunotherapy of mite allergen-relevant diseases. This study aims to characterize a novel allergen, the @entity8786 -derived serpin (Der f 27). In this study, the total RNA of @entity8786 was extracted, and the Der f 27 gene was cloned and expressed. The allergenicity of recombinant Der f 27 protein was determined by enzyme-linked immunosorbent assay, and Western-blotting with the sera of @entity565 @entity1 , and skin prick test (SPT) in @entity56 @entity1 subjects. A r-Der f 27 @entity56 @entity19 model was established. The cloned Der f 27 gene has been presented at the Gene Bank with an accession number of KM009995. The IgE levels of r-Der f 27 in the serum from r-Der f 27 SPT positive @entity56 @entity1 were 3 folds more than healthy subjects. The Der f 27 SPT positive ratewas 42.1% in 19 DM-SPT positive @entity1 . Airway hyperresponsiveness, serum specific IgE, and levels of @entity58 in the spleen cell culture supernatant were significantly increased in @entity56 @entity19 sensitized to r-Der f 27. In conclusion, Der f 27 is a new subtype of house mite allergen.

Expression, purification and characterization of Der f 27, a new allergen from XXXX .

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity565 :: ('MESH:D001249', 'Disease') :: ['asthma'] @entity8786 :: ('6954', 'Species') :: ['D. farinae'] @entity56 :: ('MESH:D004342', 'Disease') :: ['allergic asthma', 'allergic'] @entity58 :: ('16189(Tax:10090)', 'Gene') :: ['interleukin-4']
@entity8786 :: (6954,Species) :: ['dermatophagoides farinae']
