BACKGROUND: The transcription factor @entity13200 ( @entity13200 ) is a master regulatory gene necessary for the development and function of @entity11264 (+)CD25(+) regulatory T cells (Tregs). Mesenchymal stem cells ( @entity67441 ) have recently emerged as promising candidates for cell-based immunosuppression/tolerance induction protocols. Thus, we hypothesized that @entity67441 -based @entity13200 gene therapy would improve immunosuppressive capacity of @entity67441 and induce donor-specific allograft tolerance in @entity35 's liver allograft model. METHODS: The present study utilized a lentivirus vector to overexpress the therapeutic gene @entity13200 on @entity67441 . In vivo, Injections of 2  *  10(6) @entity67441 , @entity67441 or @entity13200 - @entity67441 into the portal vein were carried out immediately after liver transplantation. RESULTS: Successful gene transfer of @entity13200 in @entity67441 was achieved by lentivirus carrying @entity13200 and @entity13200 - @entity67441 engraftment in liver allograft was confirmed by fluorescence microscopy. @entity13200 - @entity67441 treatment significantly inhibited the proliferation of allogeneic ACI @entity11264 (+) T cells to splenocytes (SC) from the same donor strain or third-party BN @entity35 compared with @entity67441 . @entity13200 - @entity67441 suppressive effect on the proliferation of @entity11264 (+) T cells is contact dependent and associated with Programmed death ligand 1(PD-L1) upregulation in @entity67441 . Co-culture of @entity11264 (+) T cells with @entity13200 - @entity67441 results in a shift towards a Tregs phenotype. More importantly, @entity13200 - @entity67441 monotherapy achieved donor-specific liver allograft tolerance and generated a state of @entity11264 (+)CD25(+) @entity13200 (+) Tregs-dependent tolerance. CONCLUSION: @entity13200 -engineered @entity67441 therapy seems to be a promising and attractive cell therapy approach for inducing immunosuppression or transplant tolerance.

XXXX -modified bone marrow mesenchymal stem cells promotes liver allograft tolerance through the generation of regulatory T cells in @entity35 .

@entity35 :: ('10116', 'Species') :: ['rat'] @entity11264 :: ('24932(Tax:10116)', 'Gene') :: ['CD4'] @entity13200 :: ('317382(Tax:10116)', 'Gene') :: ['forkhead box P3', 'Foxp3'] @entity67441 :: ('312897(Tax:10116)', 'Gene') :: ['MSC', 'FUGW-MSC']
@entity13200 :: (317382(Tax:10116),Gene) :: ['Foxp3']
