OBJECTIVE: @entity213 ( @entity213 ) represents as a common @entity5 with increasing incidence in the worldwide. The fact of its poor survival rate urgently requires developing efficient predictive biomarkers for clinical use. MicroRNAs (miRNAs) recently represent as a novel direction for early diagnosis and prognosis prediction in @entity213 therapy. In this study, we comprehensively investigated the function and putative target of miRNA-451 in vitro. METHODS: The expression of miRNA-451 was detected in @entity213 tissues and cell lines by real-time PCR. Forced expression or inhibition of miRNA-451 was done by transient transfection of mimics or inhibitor of miRNA-451 into indicated cells, respectively. Cell proliferation was evaluated by cell counting and crystal staining. Afterwards, we perform western blot to verify the expression of the miRNA-451 predicted target, @entity517 , after miRNA-451 was overexpressed. RESULTS: We showed that miRNA-451 was downregulated in paired @entity213 tissues as well as in cell lines. And overexpression of miRNA-451 in cells with low endogenous expression of miRNA-451 accelerated proliferation. To the contrast, knockdown of miRNA-451 in cells with high levels of miRNA-451 significantly reduced cell growth rate. Furthermore, we used bioinformatics and cellular methods to predict and prove that @entity517 was targeted by miRNA-451, since forced expression of miRNA-451 leaded to decreased @entity517 protein expression in @entity213 cells. CONCLUSION: Our findings identify miRNA-451 as a potential biomarker and suggest a key role of miRNA-451- @entity517 pathway in @entity213 cell transformation, which could represent a novel therapeutic strategy in @entity213 treatment.

MicoRNA-451 is a novel XXXX suppressor via targeting @entity517 in head and neck squamous cell carcinomas.

@entity5 :: ('MESH:D009369', 'Disease') :: ['malignancy'] @entity517 :: ('4609', 'Gene') :: ['c-myc'] @entity213 :: ('MESH:C535575', 'Disease') :: ['Head and neck squamous cell carcinoma', 'HNSCC']
@entity5 :: (MESH:D009369,Disease) :: ['tumor']
