UNASSIGNED: The lateral line found in some amphibians and fishes has two distinctive classes of sensory organs: mechanoreceptors (neuromasts) and electroreceptors (ampullary organs). Hair cells in neuromasts can be damaged by @entity5292 antibiotics and they will regenerate rapidly afterward. @entity5292 sensitivity and the capacity for regeneration have not been investigated in ampullary organs. We treated @entity30721 ( @entity30721 ) larvae with @entity5154 and observed loss and regeneration of sensory hair cells in both organs by labeling with DASPEI and @entity4653 ( @entity4653 ). The numbers of sensory hair cells in both organs were reduced to the lowest levels at 6 hours post-treatment (hpt). New sensory hair cells began to appear at 12 hpt and were regenerated completely in 7 days. To reveal the possible mechanism for ampullary hair cell regeneration, we analyzed cell proliferation and the expression of neural placodal gene eya1 during regeneration. Both cell proliferation and eya1 expression were concentrated in peripheral mantle cells and both increased to the highest level at 12 hpt, which is consistent with the time course for regeneration of the ampullary hair cells. Furthermore, we used Texas Red-conjugated @entity801 in an uptake assay following pre-treatment with a cation channel blocker ( @entity6478 ) and found that entry of the antibiotic was suppressed in both organs. Together, our results indicate that ampullary hair cells in @entity30721 larvae can be damaged by @entity5154 exposure and they can regenerate rapidly. We suggest that the mechanisms for @entity5292 uptake and hair cell regeneration are conserved for mechanoreceptors and electroreceptors. This article is protected by copyright. All rights reserved.

@entity5154 Damage and Regeneration of Hair Cells in Both Mechanoreceptor and Electroreceptor Lateral Line Organs of the Larval @entity30721 ( XXXX ).

@entity801 :: ('MESH:D005839', 'Chemical') :: ['gentamicin'] @entity6478 :: ('MESH:D000584', 'Chemical') :: ['amiloride'] @entity5292 :: ('D000617', 'Chemical') :: ['Aminoglycoside', 'aminoglycoside'] @entity5154 :: ('MESH:D009355', 'Chemical') :: ['neomycin'] @entity30721 :: ('27689', 'Species') :: ['Acipenser baerii', 'Siberian sturgeon'] @entity4653 :: ('MESH:D004401', 'Disease') :: ['SEM', 'scanning electron microscopy']
@entity30721 :: (27689,Species) :: ['Acipenser baerii']
