UNASSIGNED: MicroRNAs (miRNAs) are non-coding RNAs of ~22 @entity2036 in length that regulate gene expression by interfering with the stability and translation of mRNAs. Their expression is regulated during development, under a wide variety of stress conditions and in several pathological processes. In nature, animals often face feast or famine conditions. We observed that subjecting early L4 larvae from @entity2199 to a 12-hr starvation period produced worms that are thinner and shorter than well-fed animals, with a decreased lipid accumulation, diminished progeny, reduced gonad size, and an increased lifespan. Our objective was to identify which of the 302 known miRNAs of @entity2199 changed their expression under starvation conditions as compared to well-fed worms by means of deep sequencing in early L4 larvae. Our results indicate that 13 miRNAs (miR-34-3p, the family of @entity69795 to miR-41-3p, miR-39-5p, miR-41-5p, miR-240-5p, miR-246-3p and miR-4813-5p) were upregulated, while 2 miRNAs (let-7-3p and miR-85-5p) were downregulated in 12-hr starved vs. well-fed early L4 larvae. Some of the predicted targets of the miRNAs that changed their expression in starvation conditions are involved in metabolic or developmental process. In particular, miRNAs of the @entity69796 family were upregulated 6-20 fold upon starvation. Additionally, we showed that the expression of @entity32264 , important in oogenesis, a validated target of @entity69795 , was downregulated when the expression of @entity69795 was upregulated. The expression of another reported target, the cell cycle regulator @entity60727 , was unchanged during starvation. This study represents a starting point for a more comprehensive understanding of the role of miRNAs during starvation in @entity2199 .

High-Throughput Profiling of XXXX Starvation-Responsive microRNAs.

@entity2199 :: ('6239', 'Species') :: ['C. elegans', 'Caenorhabditis elegans'] @entity60727 :: ('174058(Tax:6239)', 'Gene') :: ['lin-23'] @entity69796 :: ('260178(Tax:6239)', 'Gene') :: ['miR-35'] @entity69795 :: ('3565490(Tax:6239)', 'Gene') :: ['miR-35-3p'] @entity2036 :: ('MESH:D009711', 'Chemical') :: ['nucleotides'] @entity32264 :: ('172532(Tax:6239)', 'Gene') :: ['gld-1']
@entity2199 :: (6239,Species) :: ['Caenorhabditis elegans']
