An early endosome antigen previously reported by F.T. Mu could be stained on cytoplasmic vesicles of HEp-2 cells. Here, we have investigated autoantibodies against cytoplasmic vesicular antigens, especially against @entity21216 . Twelve sera were selected on the basis of cytoplasmic vesicular staining patterns of HEp-2 cells. Protein-immunoprecipitation using 35S-methionine labeled HeLa lysates, and RNA immunoprecipitation using 32P-labeled HeLa lysates were conducted to characterize the cognate antigens. Nine of 12 sera reacted with proteins in the range of 162-180 kDa, three of which were found to react specifically with the 162 kDa 35S methionine labeled recombinant @entity21216 . These proteins were not associated with common RNA. Although complete clinical information was not available, some of the @entity1 had @entity309 ( @entity309 ). In addition, the RNA-IPP results suggest that other @entity1 included one each with @entity1252 , SSc, @entity6558 , and Sj  gren's syndrome. Anti- @entity21216 antibody was found in 25%(3 of 12) of sera known to stain cytoplasmic vesicles. The reactive sera came mostly from @entity1 with @entity309 . The sera was from one case each of clinical-confirmed @entity309 , @entity1252 and Sj  gren's syndrome.

[Detection of anti- XXXX antibody in sera showing cytoplasmic vesicular staining, taken from @entity1 with connective tissue diseases].

@entity1 :: ('9606', 'Species') :: ['patients'] @entity1252 :: ('MESH:D008180', 'Disease') :: ['SLE'] @entity21216 :: ('8411', 'Gene') :: ['early endosome antigen 1'] @entity309 :: ('MESH:D001172', 'Disease') :: ['rheumatoid arthritis', 'RA'] @entity6558 :: ('MESH:D017285', 'Disease') :: ['polymyositis']
@entity21216 :: (8411,Gene) :: ['early endosome antigen 1']
