Monoassociation of germfree @entity1618 gene deficient ( @entity1618 -/-) 129SvEv but not wild-type @entity19 with @entity797 induces severe @entity1960 . Bacterial strain-specific effects on the development of @entity32 are not understood. We investigated the molecular mechanisms of @entity60325 ( @entity1 clinical isolate, colitogenic) and @entity797 @entity39256 (endogenous isolate from an @entity1618 -/- @entity19 ) in initiating @entity1960 using @entity1618 -/- @entity19 . Monoassociation of @entity1618 -/- @entity19 for 14 weeks revealed significant differences in @entity32 (3.6 +/- 0.2 and 2.4 +/- 0.6 for OG1RF and @entity39256 , respectively) (n = 5 @entity19 in each group) (histological scoring (0-4)). Consistent with the tissue pathology, gene expression of the pro-inflammatory chemokine interferon-gamma inducible protein-10 (IP-10) was significantly higher in intestinal epithelial cells (IEC) derived from @entity60325 monoassociated @entity1618 -/- @entity19 . We further compared the differentially @entity797 induced @entity1960 on the epithelial level by 2D-SDS PAGE coupled with @entity215 . Proteome analysis identified 13 proteins which were differentially regulated during disease progression in the epithelium of @entity797 -monoassociated @entity1618 -/- @entity19 . Regulation of @entity70524 / @entity70524 protein expression and @entity8837 phosphorylation was validated in primary IEC and epithelial cell lines, suggesting a protective role for @entity70524 / @entity70524 in the process of disease progression. @entity70524 / @entity70524 protein expression was further characterized in epithelial cell lines using siRNA-mediated knock-down. Our study demonstrates @entity797 -specific induction of @entity1960 in @entity1618 -/- @entity19 after 14 weeks of monoassociation. Our study suggests that @entity70524 / @entity70524 protein expression and @entity8837 activation are decreased in severe @entity1960 .

@entity797 strains differentially regulate @entity70524 / @entity70524 protein expression and @entity8837 activation in intestinal epithelial cells in the context of XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['colonic inflammation', 'chronic intestinal inflammation'] @entity60325 :: ('474186', 'Species') :: ['E. faecalis OG1RF'] @entity70524 :: ('18571(Tax:10090)', 'Gene') :: ['Alix', 'AIP1'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity215 :: ('MESH:D013771', 'Disease') :: ['MALDI-TOF MS'] @entity1618 :: ('16153(Tax:10090)', 'Gene') :: ['IL-10', 'Interleukin 10'] @entity797 :: ('1351', 'Species') :: ['Enterococcus faecalis', 'E. faecalis', 'E. faecalis strain'] @entity1960 :: ('MESH:D003092', 'Disease') :: ['chronic experimental colitis', 'colitis', 'chronic colitis'] @entity39256 :: ('111636(Tax:10090)', 'Gene') :: ['ms2'] @entity8837 :: ('26417;26413', 'Gene') :: ['ERK1/2']
@entity1960 :: (MESH:D003092,Disease) :: ['chronic experimental colitis']
