OBJECTIVE: To study the influence of multiple @entity1582 cells on differentiation of endothelial cells to form capillary-like networks and the role of @entity2252 ( @entity2252 ) in this process. METHODS: @entity1 multiple @entity1582 cell line RPMI8226 or fresh @entity1582 cells were co-cultured with @entity1 umbilical vein endothelial cells (HUVECs) in two different systems: the contact and the non-contact systems. The HUVECs cultured alone were used as control. The effects of soluble cytokine and adhesion molecule on angiogenesis in HUVECs co-cultured with MM cell were studied by modified Matrigel capillary-like networks formation assay and @entity2252 levels in co-culture system supernatant by enzyme-linked immunosorbent assay. In the contact co-culture system, the formation of capillary-like networks and the secretion of @entity2252 were detected again after MM cell was preincubated and cultured with anti- @entity2393 and anti- @entity1454 monoclonal antibodies. RESULTS: In RPMI8226 co-culture system, the number of capillary-like structure was increased in non-contact HUVECs system compared with that in monoculture, but the increase was lower than that of contact HUVECs system (75% vs. 113%). In fresh @entity1582 cells co-culture system, the numbers of capillary-like structure were 138% and 188% for the non-contact and the contact co-culture systems, respectively, above that in HUVECs cultured alone. The median @entity2252 concentrations in culture supernatants of mono-cultured, contact co-cultured with RPMI8226, non-contact co-cultured with RPMI8226, contact co-cultured with fresh @entity1582 cells and non-contact co-cultured with fresh @entity1582 cells were (12.4 +/- 5.1) ng/ ml, (38.5 +/- 8.2) ng/ml, (31.6 +/- 7.2) ng/ml, (37.1 +/- 8.7) ng/ml and(27.9 +/- 7.6) ng/ml, respectively. Either of the two adhesion molecule monoclonal antibodies reduced the capillary-like networks formation and the @entity2252 secretion in the contact co-culture system. CONCLUSION: @entity1 multiple @entity1582 cells stimulate differentiation of endothelial cells to form capillary-like networks in two different co-culture systems. @entity2252 takes part in this progress and is modulated by soluble cytokine and adhesion molecule expressed by both kinds of cells.

[ XXXX multiple @entity1582 cells stimulate differentiation of endothelial cells to form capillary-like networks in two different co-culture systems].

@entity1 :: ('9606', 'Species') :: ['human', 'Human'] @entity1582 :: ('MESH:D009101', 'Disease') :: ['myeloma'] @entity1454 :: ('3689', 'Gene') :: ['CD18'] @entity2252 :: ('627', 'Gene') :: ['brain derived neurotrophic factor', 'BDNF'] @entity2393 :: ('3688', 'Gene') :: ['CD29']
@entity1 :: (9606,Species) :: ['Human']
