UNASSIGNED: @entity2041 peroxidase (GPx) is an essential member of the antioxidant systems of living organisms and may be involved in immune defense against pathogenic invasion. In the current study, two @entity4259 -dependent @entity2041 peroxidases (AbSeGPxs) that shared 54.3% identity were identified from the disk abalone Haliotis discus discus. The open reading frames (ORFs) of AbSeGPx-a and AbSeGPx-b coded for 222 and 220 @entity1522 , respectively, with a characteristic @entity16266 residue encoded by an opal stop codon (TGA). The conserved @entity16266 insertion sequence (SECIS) element was predicted in the 3' untranslated region (UTR) of both isoforms, and they were found to form two stem-loop structures. @entity3689 comparison and phylogenetic studies revealed that the AbSeGPxs were closely related to those in other mollusk species and were evolutionarily distinct from those of other taxonomic groups. The SYBR Green qPCR was employed in investigating the transcripts of AbSeGPxs. The expression of AbSeGPxs mRNA was examined in different embryonic developmental stages and differential expression patterns for AbSeGPx-a and AbSeGPx-b were noted. Meanwhile, the highest expression of AbSeGPxs was detected in the hepatopancreas of healthy adult animals. Next, transcriptional levels were profiled in hemocytes of adults to determine the immune responses of AbSeGPxs to microbial infections. The results revealed the significant up-regulation of AbSeGPx-a in a time-dependent manner after bacterial ( @entity5125 and @entity8966 ) and viral (viral hemorrhagic septicemia virus) infections. Consequently, these findings indicate that AbSeGPx-a and AbSeGPx-b might be involved in the embryonic development of disk abalone and the regulation of immune defense system of adult animals.

Two variants of @entity4259 -dependent XXXX peroxidase from the disk abalone Haliotis discus discus: Molecular characterization and immune responses to bacterial and viral stresses.

@entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity8966 :: ('670', 'Species') :: ['Vibrio parahaemolyticus'] @entity4259 :: ('MESH:D012643', 'Chemical') :: ['selenium'] @entity2041 :: ('MESH:D005978', 'Chemical') :: ['glutathione', 'Glutathione'] @entity5125 :: ('1639', 'Species') :: ['Listeria monocytogenes'] @entity16266 :: ('MESH:D017279', 'Chemical') :: ['selenocysteine'] @entity3689 :: ('D000596', 'Chemical') :: ['Amino acid']
@entity2041 :: (MESH:D005978,Chemical) :: ['glutathione']
