BACKGROUND: Subcutaneous xenograft is a common method to establish animal models of @entity1 @entity1721 ( @entity1721 ). However, the growth microenvironment of transplanted @entity5 is different from @entity5 . Orthotopic implantation models can provide more biologically relevant context in which to study the disease. So far, an orthotopic implantation model of @entity1721 has rarely been reported. METHODS: The @entity1 @entity1721 cell line KYSE30 was transfected with pLVX-Luciferase plasmids. KYSE30-Luciferase cells were isolated and injected into the flanks of @entity19 to develop a subcutaneous @entity5 . An orthotopic implantation model was established using the fragments derived from the subcutaneous @entity5 . Fluorescence imaging was used to observe the development of the orthotopic @entity5 . @entity1710 and @entity1711 staining was performed to evaluate the invasion and @entity3 of the @entity5 . RESULTS: KYSE30 cells were successfully transfected with pLVX-Luciferase plasmids. A @entity5 was developed in all @entity19 . The @entity19 experienced body @entity353 . The @entity5 infiltrated into the esophageal muscularis propria. However, neither distant organ nor lymph node @entity3 was found. The progression of the @entity5 was monitored by in vivo fluorescence imaging. CONCLUSION: The orthotopic implantation model can be established by sewing the fragments of @entity1 @entity1721 to the abdominal esophagus of a nude @entity19 . The progression of an orthotopic implantation @entity5 can be monitored in real time by in vivo fluorescence imaging.

New orthotopic implantation model of @entity1 @entity1721 in athymic XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity353 :: ('MESH:D015431', 'Disease') :: ['weight loss'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'primary tumors', 'implanted tumor', 'primary tumor', 'tumors'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'nude mice'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity1721 :: ('MESH:C562729', 'Disease') :: ['esophageal squamous cell carcinoma', 'ESCC'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['Hematoxylin'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis']
@entity19 :: (10090,Species) :: ['nude mice']
