OBJECTIVES: Microbial @entity7944 ( @entity7944 ), such as @entity4334 -binding oligomerization domains (NODs), are essential for @entity1 innate immune response. This study was designed to determine the effect of @entity18506 and @entity6820 agonist on innate immune responses and antitumor activity in @entity957 ( @entity957 ) cells. MATERIALS AND METHODS: NODs expression was examined by RT-PCR, and @entity687 production by NODs agonist was examined by ELISA. Western blot analysis was performed to determine the MAPK activation in response to their agonist. Cell proliferation was determined by @entity98 assay. Flow cytometry and Western blot analysis were performed to determine the @entity7192 -induced cell death. RESULTS: The levels of NODs were apparently expressed in @entity957 cells. NODs agonist, Tri-DAP and @entity7192 , led to the production of @entity687 and MAPK activation. @entity6820 agonist, @entity7192 , inhibited the proliferation of YD-10B cells in a dose-dependent manner. Also, the ratio of @entity4404 -positive cells and cleaved @entity9190 was increased by @entity7192 treatment in YD-10B cells, suggesting that @entity7192 -induced cell death in YD-10B cells may be owing to apoptosis. CONCLUSIONS: Our results indicate that NODs are functionally expressed in @entity957 cells and can trigger innate immune responses. In addition, @entity6820 agonist inhibited cell proliferation and induced apoptosis. These findings provide the potential value of @entity7192 as novel candidates for antitumor agents of @entity957 .

@entity6820 ( XXXX ) activation induces apoptosis of @entity1 @entity957 cells.

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity4404 :: ('308', 'Gene') :: ['Annexin V'] @entity7192 :: ('CHEBI:59414', 'Chemical') :: ['MDP'] @entity957 :: ('MESH:D002294', 'Disease') :: ['OSCC', 'oral squamous cell carcinoma'] @entity7944 :: ('MESH:C536309', 'Disease') :: ['PRRs', 'Pattern-recognition receptors'] @entity687 :: ('3576', 'Gene') :: ['IL-8'] @entity6820 :: ('64127', 'Gene') :: ['NOD2'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity9190 :: ('1302', 'Gene') :: ['PARP'] @entity4334 :: ('D009711', 'Chemical') :: ['nucleotide'] @entity18506 :: ('10392', 'Gene') :: ['NOD1']
@entity6820 :: (64127,Gene) :: ['NOD2']
