INTRODUCTION: Mechanisms of detachment of fetal membrane after parturition in @entity291 are poorly understood. Glucocorticoids trigger the initiation of parturition and may facilitate the placental maturation. We compared the disappearance of trophoblast binucleate cells (BNCs) and expression of transforming growth factor-b (TGFB) in term placentomes between spontaneous and induced parturition to investigate the influences of glucocorticoids on the placental maturity. METHODS: @entity291 were delivered spontaneously (SP group) or after the administration of @entity3009 ( @entity22517 ) F2a ( @entity22517 group); @entity619 , PGF2a, and @entity11111 ( @entity9170 group); and @entity2995 , PGF2a, and @entity5935 ( @entity40006 group) and placentomes were collected immediately after parturition. The number of BNCs in @entity1710 and @entity1711 stained section was examined. Protein localization and mRNA levels of TGFB and its receptor (TGFBR) were analyzed using immunohistochemistry and qRT-PCR, respectively. RESULTS: @entity12574 is characteristically localized in the maternal septum in caruncle in contrast to @entity12271 and @entity68293 , which are mainly found in cotyledonary villi and maternal epithelial cells. @entity68294 and @entity68295 colocalized in BNCs. The number of BNCs was lower in the SP group than in @entity22517 and @entity9170 groups. mRNA levels of @entity12574 , @entity68294 and @entity68295 in the SP group differed from @entity22517 and @entity9170 groups. There was no difference between SP and @entity40006 groups in all analyses. DISCUSSION: These results indicate that parturition inductions using PGF2a or @entity619 were not able to induce disappearance of BNCs and change of TGFB signaling. Results in the @entity40006 group suggest that investigation into types, dose, and dosage schedule of glucocorticoids may facilitate placental maturation.

Localization of TGF-b and TGF-b receptor in XXXX term placentome and expression differences between spontaneous and induced parturition.

@entity2995 :: ('MESH:D014222', 'Chemical') :: ['triamcinolone acetonide'] @entity9170 :: ('D003907', 'Chemical') :: ['DEX'] @entity68295 :: ('535376(Tax:9913)', 'Gene') :: ['TGFBR2'] @entity5935 :: ('MESH:D001623', 'Chemical') :: ['betamethasone'] @entity11111 :: ('MESH:D004964', 'Chemical') :: ['estriol'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity3009 :: ('CHEBI:26333', 'Chemical') :: ['prostaglandin'] @entity40006 :: ('CHEBI:17750', 'Chemical') :: ['BET'] @entity619 :: ('MESH:D003907', 'Chemical') :: ['dexamethasone'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hematoxylin'] @entity12574 :: ('282089(Tax:9913)', 'Gene') :: ['TGFB1'] @entity68293 :: ('538957(Tax:9913)', 'Gene') :: ['TGFB3'] @entity68294 :: ('282382(Tax:9913)', 'Gene') :: ['TGFBR1'] @entity291 :: ('9913', 'Species') :: ['Cows', 'cattle'] @entity12271 :: ('534069(Tax:9913)', 'Gene') :: ['TGFB2'] @entity22517 :: ('D010400', 'Chemical') :: ['PG']
@entity291 :: (9913,Species) :: ['bovine']
