BACKGROUND AND AIM: Epithelial-mesenchymal transition (EMT) of biliary epithelial cells (BECs) plays an important role in @entity82 . This study investigated the effects of @entity3413 on the lipopolysaccharide (LPS)-induced EMT and related signal pathways in BECs. METHODS: BECs were exposed to LPS (2     g/mL) or @entity2533 (5   ng/ml) for 5   days. The EMT was assessed by a gain of mesenchymal cell markers ( @entity2277 , @entity6076 , slug, and @entity8945 ) and a loss of epithelial cell markers ( @entity5962 ). The effects of @entity3413 on the EMT induced by LPS or @entity2533 were determined by the changes in the levels of EMT markers and @entity2939 and in the JNK, @entity1435 , and NF-kB signaling pathways. RESULTS: Compared with the BECs treated with LPS alone, co-treatment with @entity3413 and LPS induced an increase in the expression of @entity5962 and decreases in the expression levels of mesenchymal cell markers. The LPS-induced @entity2939 expression level was slightly decreased by co-treatment with @entity3413 . LPS-induced BEC growth was markedly inhibited by co-treatment with @entity3413 . Furthermore, pretreatment with @entity3413 inhibited the LPS-induced EMT in BECs by downregulating NF-kB and JNK phosphorylation. The suppressive effects of @entity3413 pretreatment on the induction of the EMT by @entity2533 were also demonstrated in H69 cells. CONCLUSIONS: Our results demonstrate that LPS or @entity2533 promote the EMT in BECs that that pretreatment with @entity3413 inhibited the induced EMT by downregulating @entity2939 and NF-kB phosphorylation. This finding suggests that @entity3413 can be considered a new agent for preventing @entity82 associated with the EMT of BECs.

Statin pretreatment inhibits the LPS-induced EMT via the downregulation of XXXX and NF-kB in human biliary epithelial cells.

@entity82 :: ('MESH:D005355', 'Disease') :: ['biliary fibrosis'] @entity3413 :: ('MESH:D019821', 'Chemical') :: ['simvastatin'] @entity2939 :: ('7099', 'Gene') :: ['TLR4'] @entity5962 :: ('999', 'Gene') :: ['E-cadherin'] @entity2533 :: ('7040', 'Gene') :: ['TGF-b1'] @entity2277 :: ('7431', 'Gene') :: ['vimentin'] @entity1435 :: ('1432', 'Gene') :: ['p38'] @entity8945 :: ('7291', 'Gene') :: ['twist-1'] @entity6076 :: ('1000', 'Gene') :: ['N-cadherin']
@entity2939 :: (7099,Gene) :: ['TLR4']
