BACKGROUND: Oxidative stress and @entity7499 are important elements in the pathogenesis of inflammatory diseases and @entity5 . Our aim was to evaluate the role of both and of antioxidant capacity in the @entity420 sequence in @entity355 . METHODS: In mucosal specimens from 59 @entity1 grouped as having symptomatic @entity354 , @entity355 , or @entity420 in the esophagus, plus controls, we measured @entity3335 activity, superoxidase dismutase activity, @entity2041 content, and total aromatic DNA adducts. To evaluate blood vessel densities and angioarchitecture, we used immunohistochemistry and a modified whole-mount technique. Sections were stained with endothelium-specific markers and smooth muscle cell actin. RESULTS: The @entity189 sequence revealed progressively increased oxidative stress (increased @entity3335 activity), decreased antioxidant capacity ( @entity2041 content), and simultaneous formation of DNA adducts. Pooled data show a negative correlation between @entity2041 content and DNA adducts (-0.28; P =.05). This sequence was also characterized by increased intensity in microvessels and an increasing percentage of immature blood vessels. In addition, the whole-mount technique offered 3-dimensional evidence that the rich new vascular bed is highly abnormal, with repeated twists, bends, or turns, even in nonmalignant Barrett esophagus. CONCLUSIONS: Increased oxidative stress, decreased antioxidant capacity, and a negative correlation between @entity2041 content and DNA adduct formation indicate a link between oxidative stress and @entity355 . Simultaneously, this transformation acquires angiogenic capacity, strong neovascularization, and abnormal angioarchitecture.

Simultaneous progression of oxidative stress and @entity7499 in XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity3335 :: ('4353', 'Gene') :: ['myeloperoxidase'] @entity7499 :: ('MESH:D016510', 'Disease') :: ['angiogenesis'] @entity355 :: ('MESH:D001471', 'Disease') :: ['Barrett epithelium', 'malignant transformation of Barrett epithelium'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity2041 :: ('MESH:D005978', 'Chemical') :: ['glutathione'] @entity189 :: ('MESH:D002277', 'Disease') :: ['reflux disease-metaplasia-carcinoma'] @entity354 :: ('MESH:D005764', 'Disease') :: ['gastroesophageal reflux disease'] @entity420 :: ('MESH:D000230', 'Disease') :: ['metaplasia-dysplasia-adenocarcinoma', 'adenocarcinoma']
@entity5 :: (MESH:D009369,Disease) :: ['malignant transformation of Barrett esophagus']
