UNASSIGNED: Multiphenotype genome-wide association studies (GWAS) may reveal pleiotropic genes, which would remain undetected using single phenotype analyses. Analysis of large pedigrees offers the added advantage of more accurately assessing trait heritability, which can help prioritise genetically influenced phenotypes for GWAS analysis. In this study we performed a principal component analysis (PCA), heritability (h2) estimation and pedigree-based GWAS of 37 @entity65 -related phenotypes in 330 related individuals forming a large pedigree from the Norfolk Island genetic isolate. PCA revealed 13 components explaining >75% of the total variance. Nine components yielded statistically significant h2 values ranging from 0.22 to 0.54 (P<0.05). The most heritable component was loaded with 7 phenotypic measures reflecting metabolic and @entity8 . A GWAS of this composite phenotype revealed statistically significant associations for 3 adjacent SNPs on chromosome 1p22.2 (P<1x10-8). These SNPs form a 42kb haplotype block and explain 11% of the genetic variance for this renal function phenotype. Replication analysis of the tagging SNP ( @entity68790 ) in an independent US cohort supports the association (P = 0.000011). Blood transcript analysis showed 35 genes were associated with @entity68790 (P<0.05). Gene set enrichment analysis of these genes revealed the most enriched pathway was @entity17262 metabolism (P = 0.0015). Overall, our findings provide convincing evidence for a major pleiotropic effect locus on chromosome 1p22.2 influencing risk of @entity8 via @entity17262 metabolism pathways in the Norfolk Island population. Further studies are now warranted to interrogate the functional relevance of this locus in terms of renal pathology and @entity65 risk.

A Phenomic Scan of the Norfolk Island Genetic Isolate Identifies a Major Pleiotropic Effect Locus Associated with XXXX Markers.

@entity68790 :: ('rs1396315', 'SNP') :: ['rs1396315'] @entity8 :: ('MESH:D007674', 'Disease') :: ['renal dysfunction'] @entity17262 :: ('D011687', 'Chemical') :: ['purine'] @entity65 :: ('MESH:D002318', 'Disease') :: ['cardiovascular disease']
@entity8 :: (MESH:D007674,Disease) :: ['Metabolic and Renal Disorder']
