UNASSIGNED: Ovarian steroidogenesis is embedded in a sensitive network of regulatory mechanisms crucial for @entity1 fertility. The endocannabinoid system (ECS) represents an intrinsic modulating system involved in the regulation of endocrine functions. In the present study we characterized the ECS in the @entity1 granulosa cell line KGN and its impact on gonadotropin sensitivity and @entity479 hormone synthesis under basal and FSH- stimulated conditions. Expression studies were performed and @entity53 was measured. @entity6510 , @entity12332 , DAGL, @entity10131 , @entity70087 , @entity44666 , @entity44665 and @entity13812 were expressed without FSH-dependent effects. Treatment with selective cannabinoid receptor agonists reduced basal but not FSH-stimulated @entity53 and @entity4819 . @entity1420 was not altered by ECS manipulation. @entity6510 agonist changed the expression of miRNAs associated with granulosa cell function, e.g. @entity21176 , miR-24, miR-181a and @entity28756 . Present data indicate a modulating role of the intrinsic ovarian ECS in the regulation of @entity53 synthesis.

The endocannabinoid system in the XXXX granulosa cell line KGN.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1420 :: ('MESH:D011374', 'Chemical') :: ['Progesterone'] @entity4819 :: ('1588', 'Gene') :: ['CYP19'] @entity10131 :: ('2166', 'Gene') :: ['FAAH'] @entity6510 :: ('1268', 'Gene') :: ['CB1'] @entity44665 :: ('222236;2822', 'Gene') :: ['NAPE-PLD'] @entity13812 :: ('7442', 'Gene') :: ['TRPV1'] @entity21176 :: ('407010', 'Gene') :: ['miR-23a'] @entity12332 :: ('1269', 'Gene') :: ['CB2'] @entity53 :: ('MESH:D004958', 'Chemical') :: ['estradiol'] @entity44666 :: ('11343', 'Gene') :: ['MAGL'] @entity28756 :: ('407037', 'Gene') :: ['miR-320a'] @entity70087 :: ('9290', 'Gene') :: ['GPR55'] @entity479 :: ('D013256', 'Chemical') :: ['steroid']
@entity1 :: (9606,Species) :: ['human']
