@entity2 is a severe disease threatening @entity1 health worldwide. Distant hematogenous metastasis results in poor prognosis and @entity2 @entity1 . In the present study, we investigated the effect of circulatory platelets (PLTs) on hematogenous metastasis of @entity1149 ( @entity1149 ). Laser scanning confocal microscopy was employed to assay the expression of @entity8729 in @entity2 tissue, paracancerous tissue and distant tissue, respectively. Meanwhile, fluorescence-activated cell sorting (FACS) was used to determine @entity8729 activation in peripheral blood. Purified PLTs were co-cultured with A549 cells and @entity1 vascular endothelial cells (HuvECs). Subsequently, the formation of @entity2 cell complexes and their effects on rolling and adhesion of A549 on the surface of vascular endothelium were assayed. Integrin a3, a5, b1, @entity2071 and @entity4725 mRNAs and proteins were measured by reverse RT-PCR and western blot analysis, respectively. The expression of @entity8729 in lung @entity420 tissue was significantly stronger compared to that in paracancerous and distant tissues. @entity8729 activation in peripheral @entity2220 was markedly enhanced. The rolling rate of A549 on HuvECs was significantly slowed down after co-culture of activated PLTs and A549 cells. The mRNA and protein levels of integrin a3, a5, b1, @entity2071 and @entity4725 were significantly increased after the co-culture. In conclusion, the @entity2 cell complexes protected the @entity2 cells from mechanical injury under blood flow. Furthermore, up-regulated integrin a3, a5, b1 and endothelial cell adhesion molecules @entity2071 and @entity4725 promoted the adhesion of A549 on vascular endothelial cells, which may be responsible for hematogenous metastasis of @entity2 .

XXXX -mediated platelet activation promotes adhesion of @entity1149 cells on vascular endothelial cells under flow.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity4725 :: ('7412', 'Gene') :: ['VCAM-1'] @entity1149 :: ('MESH:D002289', 'Disease') :: ['NSCLC', 'non-small cell lung carcinoma'] @entity2 :: ('MESH:D008175', 'Disease') :: ['PLT-lung cancer', 'Lung cancer', 'lung cancer', 'death of lung cancer'] @entity8729 :: ('6403', 'Gene') :: ['P-selectin'] @entity2071 :: ('3383', 'Gene') :: ['ICAM-1'] @entity420 :: ('MESH:D000230', 'Disease') :: ['adenocarcinoma'] @entity2220 :: ('MESH:C538231', 'Disease') :: ['blood in lung adenocarcinoma']
@entity8729 :: (6403,Gene) :: ['P-selectin']
