Exposure to air pollution particles can be associated with increased @entity1 morbidity and mortality. The mechanism(s) of @entity651 remains unknown. We tested the hypothesis that lung exposure to oil @entity238 ash (an emission source air pollution particle) causes in vivo free radical production. Electron spin resonance (ESR) in conjunction with the spin trap @entity48922 ( @entity48922 ) was used to detect radical adducts. @entity35 were instilled with 500 micrograms of either oil @entity238 ash or saline. Twenty-four hours later, ESR spectroscopy of the @entity3636 extract from lungs of animals exposed to the oil @entity238 ash gave a spectrum consistent with a @entity505 -centered radical adduct (hyperfine coupling constants alpha N = 15.0 G and alpha H beta = 2.5 G), while those spectra from lungs instilled with saline revealed a much weaker signal. This signal was reproduced by instilling animals with the soluble fraction of the oil @entity238 ash, which contains soluble metal compounds. The same signal was observed after instillation of either a mixture of @entity5336 , @entity3406 , and @entity68 sulfates or VOSO4 alone. We conclude that, after instillation of an air pollution particle in the @entity35 , ESR analysis of lung tissue demonstrates in vivo free radical production. This generation of free radicals appears to be associated with soluble metals in the oil @entity238 ash.

In vivo evidence of free radical formation in the XXXX lung after exposure to an emission source air pollution particle.

@entity1 :: ('9606', 'Species') :: ['human'] @entity3406 :: ('MESH:D009532', 'Chemical') :: ['nickel'] @entity68 :: ('MESH:D007501', 'Chemical') :: ['iron'] @entity48922 :: ('MESH:C043438', 'Chemical') :: ['alpha-(4-pyridyl 1-oxide)-N-tert-butylnitrone', '4-POBN'] @entity3636 :: ('MESH:D002725', 'Chemical') :: ['chloroform'] @entity651 :: ('MESH:D055370', 'Disease') :: ['lung injury'] @entity505 :: ('MESH:D002244', 'Chemical') :: ['carbon'] @entity35 :: ('10116', 'Species') :: ['rat', 'Rats'] @entity238 :: ('7227', 'Species') :: ['fly'] @entity5336 :: ('MESH:D014639', 'Chemical') :: ['vanadium']
@entity35 :: (10116,Species) :: ['rat']
