UNASSIGNED: Enhanced respiration during ripening in climacteric fruits is sometimes associated with an uncoupling between the @entity855 synthesis and the mitochondrial electron transport chain. While the participation of two energy-dissipating systems, one of which is mediated by the alternative oxidase (AOX) and the other mediated by the uncoupling protein (UCP), has been linked to fruit ripening, the relation between the activation of both mitochondrial uncoupling systems with the transient increase of @entity1786 synthesis ( @entity1786 peak) remains unclear. To elucidate this question, @entity1786 emission and the two uncoupling (AOX and UCP) pathways were monitored in harvested @entity2732 fruit during the ripening, from green to fully yellow skin. The results confirmed the typical climacteric behavior for @entity2732 fruit: an initial increase in endogenous @entity1786 emission which reaches a maximum (peak) in the intermediate ripening stage, before finally declining to a basal level in ripe fruit. Respiration of intact fruit also increased and achieved higher levels at the end of ripening. On the other hand, in purified mitochondria extracted from fruit pulp the total respiration and respiratory control decrease while an increase in the participation of AOX and UCP pathways was markedly evident during @entity2732 ripening. There was an increase in the AOX capacity during the transition from green fruit to the intermediate stage that accompanied the transient @entity1786 peak, while the @entity3612 consumption triggered by UCP activation increased by 80% from the beginning to end stage of fruit ripening. Expression analyses of AOX (AOX1 and 2) and UCP (UCP1-5) genes revealed that the increases in the AOX and UCP capacities were linked to a higher expression of AOX1 and UCP (mainly UCP1) genes, respectively. In silico promoter analyses of both genes showed the presence of @entity1786 -responsive cis-elements in UCP1 and UCP2 genes. Overall, the data suggest a differential activation of AOX and UCP pathways in regulation related to the @entity1786 peak and induction of specific genes such as AOX1 and UCP1.

Involvement of AOX and UCP pathways in the post-harvest ripening of XXXX fruits.

@entity2732 :: ('3649', 'Species') :: ['papaya'] @entity3612 :: ('D013481', 'Chemical') :: ['O2'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP'] @entity1786 :: ('MESH:C036216', 'Chemical') :: ['ethylene']
@entity2732 :: (3649,Species) :: ['papaya']
