Toll like receptor (TLR) 7, 8 and 9 are intracellular TLRs which play important roles in host immune defense against @entity778 pathogens. In this study, TLR7, 8 and 9 were identified from golden pompano ( @entity69398 ), a marine teleost with great economic values. Sequence analysis revealed that the three TLRs contained several conserved characteristic features, including signal peptides, 25 @entity368 -rich repeat (LRR) motifs, a transmembrane domain and a TIR motif. These three TLRs shared high sequence identity and similarity with their counterparts from other teleosts. The phylogenetic tree analysis showed the three TLRs were clustered well with their piscine counterparts, confirming the correctness of their nomenclatures and closed relationships during evolution. Quantitative real-time PCR revealed that the three TLRs were ubiquitously expressed in all the tested tissues from normal pompano, with high expression in spleen and head kidney, indicating their role in immune reaction. Further, pompano TLR7 and TLR8 was up-regulated in spleen and head kidney from 12  h to 48  h following @entity17761 :C challenge, but remained no changes to Vibrio @entity281 . In contrast, pompano TLR9 could be induced by V.  @entity281 but remained apathetic to polyI:C challenge. These results indicated that pompano TLR7, 8 and 9 might have distinct roles in response to @entity778 pathogens. Our results provided the basis for further study on ligand specificity and signaling pathways of fish TLRs which are required for elucidating the immune functions of fish TLRs.

Molecular cloning and expression analysis of toll-like receptor genes (TLR7, TLR8 and TLR9) of golden pompano ( XXXX ).

@entity778 :: ('MESH:D015658', 'Disease') :: ['bacterial or virus'] @entity368 :: ('CHEBI:25017', 'Chemical') :: ['leucine'] @entity69398 :: ('173339', 'Species') :: ['Trachinotus ovatus'] @entity281 :: ('MESH:D007239', 'Disease') :: ['alginilyticus infection'] @entity17761 :: ('MESH:D011069', 'Chemical') :: ['polyI']
@entity69398 :: (173339,Species) :: ['Trachinotus ovatus']
