OBJECTIVE: Previous studies have shown that chronic cardiac denervation impairs myocardial @entity413 oxidation. To investigate this further we tested whether the tissue content of @entity413 transporters, activity of glycolytic enzymes or metabolic capacity of @entity2388 dehydrogenase were altered. Moreover, we investigated whether the decline in @entity413 utilization was associated with an upregulation of proteins and enzymes involved in @entity844 handling. Chronic cardiac denervation results also in decreased left ventricular efficiency. We explored whether alterations in mitochondrial properties could be held responsible for this phenomenon. METHODS: Twelve adult @entity235 were included in the study. In 6 of them chronic cardiac denervation was accomplished by surgical ablation of the extrinsic nerve fibers. The other 6 @entity235 were sham-operated. Biopsies were obtained from the left ventricle after 4-5 weeks of denervation. The content or enzymatic activity of proteins involved in @entity844 and @entity413 handling was assessed. Features of @entity2227 oxidation were measured in freshly isolated mitochondria. RESULTS: The content or activity of a set of @entity844 handling proteins did not change during chronic cardiac denervation. In contrast GLUT1 content significantly increased in the chronically denervated left ventricle, while the active form of @entity2388 dehydrogenase declined (p < 0.05). @entity2227 oxidation characteristics in freshly isolated mitochondria were not affected by chronic denervation. CONCLUSION: The impairment of @entity413 oxidation in the chronically denervated myocardium is most likely caused by a decline of @entity2388 dehydrogenase in its active form. It is unlikely that the decrease in work efficiency is caused by alterations in mitochondrial properties.

Metabolic alterations in the chronically denervated XXXX heart.

@entity844 :: ('CHEBI:35366', 'Chemical') :: ['fatty acid'] @entity2227 :: ('CHEBI:29987', 'Chemical') :: ['glutamate', 'Glutamate'] @entity235 :: ('9615', 'Species') :: ['dogs'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity2388 :: ('MESH:D019289', 'Chemical') :: ['pyruvate']
@entity235 :: (9615,Species) :: ['dog']
