The influence of bacterial hemoglobin, VHb, on dechlorination and degradation of @entity44175 ( @entity44176 ) by recombinant @entity6402 sp. under variable @entity26 availability with an initial dissolved @entity26 concentration of 0.27 mM-0.72 mM was investigated in batch and continuous culture. Ability to express VHb was provided to recombinant @entity6402 by transformation with the VHb gene, vgb, on plasmid pSC160. 100% of 0.5 mM @entity11643 was degraded in cultures with 85% and 70% of total volume as headspace air in closed reactors by both wild type and recombinant @entity6402 . The recombinant cultures were able to dechlorinate and degrade 100% of the @entity44176 in less than 48 hours at 30 degrees C compared to more than 120 hours for wild type cultures. The rate and extent of @entity11643 degradation by recombinant cultures with 40% of total volume as headspace air was higher than those achieved by wild type cells at the end of the 168 hours of incubation period, 98 and 73%, respectively. The @entity2865 released: @entity11643 degraded molar ratio for cultures with 40% of total volume headspace air was nearly stoichiometric (molar ratio = 1.0) for recombinant strains, whereas it was non-stoichiometric (molar ratio = 0.24) for wild type cells. The results suggest a suicidal meta-pathway for wild type cells and a complete dechlorination and degradation pathway for recombinant cells under @entity519 conditions. The degradation and dechlorination ability of both types of cells was also investigated in continuous reactor studies by varying the dilution rate under @entity519 conditions. Regarding potential of the recombinant strain for @entity44176 degradation in either open ecosystems or closed bioreactor bioremediation systems, the stability of the plasmid containing vgb in the recombinant cells was also studied; the plasmid was 100% stable at 0.025 h(-1) dilution rate (approximately 1.7 d hydraulic retention time), even after one month.

Biodegradation of @entity44175 by recombinant @entity6402 expressing Vitreoscilla hemoglobin under variable levels of XXXX availability.

@entity11643 :: ('CHEBI:3796', 'Chemical') :: ['CBA'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity519 :: ('MESH:D000860', 'Disease') :: ['hypoxic'] @entity44176 :: ('MESH:C111607', 'Chemical') :: ['2-CBA'] @entity44175 :: ('MESH:C060415', 'Chemical') :: ['2-chlorobenzoate'] @entity2865 :: ('CHEBI:17996', 'Chemical') :: ['chloride'] @entity6402 :: ('292', 'Species') :: ['Burkholderia']
@entity26 :: (MESH:D010100,Chemical) :: ['oxygen']
