Flow cytometry was used to examine the effect of hydrodynamic forces in a stirred tank bioreactor on the @entity10793 and @entity1116 receptor surface content of HL60 ( @entity1 @entity2799 ) cells. A step increase in @entity2146 rate from 80 to 400 rpm reduced the HL60 cell apparent growth rate and increased the @entity10793 receptor surface content per cell, on average, by 95%. In contrast, this step increase in @entity2146 rate to 400 rpm decreased the @entity1116 receptor surface content per cell, on average, by 10%. The protective effects of 0.1% Methocel A15LV, @entity968 ( @entity970 ), and @entity4832 ( @entity939 ) on @entity10793 and @entity1116 receptor surface content were examined under @entity2146 at 300 rpm in parallel 2 L bioreactor runs. The average @entity1116 receptor surface content was unaffected by the presence of Methocel A15LV or @entity970 , while @entity939 had a slight protective effect. In contrast, in terms of @entity10793 receptor content, HL60 cells agitated at 300 rpm with Methocel A15LV, @entity970 , or @entity939 behaved like cells agitated at 80 rpm with no media additives (McDowell and Papoutsakis, 1998). That is, Methocel A15LV, @entity970 , and @entity939 prevented the transduction of mechanical forces which affect @entity10793 cell content. HL60 cells cultured with 0.1% A15LV, @entity970 or @entity939 under conditions of mild @entity2146 (60 rpm) in spinner flasks exhibited @entity413 consumption and @entity880 production rates that were approximately 20% lower than values of cultures containing no additive. Under conditions of @entity2146 at 300 rpm in the 2 L bioreactor, the presence of A15LV, @entity970 , and @entity939 reduced the HL60 @entity413 consumption and @entity880 production rates by approximately 50%. Thus, media additives can dramatically reduce @entity880 accumulation in agitated bioreactors due to cell growth, in addition to providing protection from cellular injury.

Effects of methocel A15LV, XXXX , and @entity4832 on @entity10793 and @entity1116 receptor surface content and metabolism of HL60 cells cultured in stirred tank bioreactors.

@entity10793 :: ('290', 'Gene') :: ['CD13'] @entity1 :: ('9606', 'Species') :: ['human'] @entity2799 :: ('MESH:D015473', 'Disease') :: ['promyelocytic leukemia'] @entity939 :: ('CHEBI:17246', 'Chemical') :: ['PVA'] @entity4832 :: ('MESH:D011142', 'Chemical') :: ['polyvinyl alcohol'] @entity968 :: ('MESH:D011092', 'Chemical') :: ['polyethylene glycol'] @entity2146 :: ('MESH:D011595', 'Disease') :: ['agitation'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity970 :: ('CHEBI:46793', 'Chemical') :: ['PEG'] @entity1116 :: ('945', 'Gene') :: ['CD33']
@entity968 :: (MESH:D011092,Chemical) :: ['polyethylene glycol']
