OBJECTIVE: To access the production of a novel uterine luminal fluid 250 kD protein (ULF-250) during the various phases of the estrous cycle in relation to @entity172 concentration, and to validate that the production and secretion of ULF-250 are regulated by @entity53 and @entity1420 . METHODS: An immunohistochemical method was used to localize ULF-250 in @entity35 uteri during each phase of the estrous cycle, and in uteri of ovariectomized @entity35 treated with @entity53 and @entity1420 . RESULTS: Positive immunostaining of ULF-250 occurred in the epithelial cells of the uterus at all phases of the estrous cycle; whereas the stroma was immunonegative. During the proestrus phase of the cycle, the glandular epithelial cells and glandular luminal content were stained strongly. During the estrus phase of the cycle, intense staining occurred in the glandular and uterine luminal epithelial cells, including the luminal content of the glands. In the metestrus phase of the cycle, only uterine epithelial cells were stained; during the diestrus phase, intense staining of the secreted contents of the uterine cavity and the glandular lumen occurred. The distribution of ULF-250 in the uteri of ovariectomized female @entity35 treated with @entity53 alone and @entity53 plus @entity1420 were examined. In both groups, intense staining of the glandular luminal epithelial cells of the uterine endometrium occurred; in the @entity53 -treated animals, only the luminal contents were stained. The present findings suggest that @entity1420 inhibits the secretion of ULF-250 that is stimulated by @entity53 . CONCLUSIONS: ULF-250 is produced by the glandular and luminal epithelial cells of the uterine endometrium and fluctuates with the phases of the estrous cycle. Its production is stimulated by @entity53 and its secretion is regulated by @entity1420 .

Immunolocalization of uterine luminal fluid protein (ULF-250) in XXXX uterus.

@entity172 :: ('MESH:D004967', 'Chemical') :: ['estrogen'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity1420 :: ('MESH:D011374', 'Chemical') :: ['progesterone'] @entity53 :: ('MESH:D004958', 'Chemical') :: ['estradiol']
@entity35 :: (10116,Species) :: ['rat']
