BACKGROUND: Toxoplasmosis was recently included as a neglected disease by the Center for Disease Control. @entity17577 is one clinical presentation of congenital or acquired @entity281 . The laboratory diagnosis is being used worldwide to support the clinical diagnosis and imaging. The aim of this study was to evaluate the use of serology and molecular methods to monitor acute OT in immunocompetent @entity1 during treatment. METHODS: Five immunocompetent @entity1 were clinically diagnosed with acute OT. The clinical evaluation was performed by ophthalmologic examination using the Early Treatment @entity6 Study, best-corrected visual acuity, slit lamp biomicroscopy, fundoscopic examination with indirect binocular ophthalmoscopy color fundus photography, @entity941 angiography and spectral optical coherence tomography (OCT). Serology were performed by ELISA (IgA, IgM, IgG) and confirmed by ELFA (IgG, IgM). Molecular diagnoses were performed in peripheral blood by cPCR using the @entity3204 B1 gene as the marker. Follow-up exams were performed on day +15 and day +45. RESULTS: Only five non-immunocompromised male @entity1 completed the follow up and their data were used for analysis. The mean age was 41.2      11.3  years (median: 35; range 31-54  years). All of them were positive for IgG antibodies but with different profiles for IgM and IgA, as well as PCR. For all @entity1 the OCT exam showed active lesions with the inner retinal layers being abnormally hyper-reflective with full-thickness disorganization of the retinal reflective layers, which assumed a blurred reflective appearance and the retina was thickened. CONCLUSIONS: The presence of IgA and IgM confirmed the acute @entity281 and thus was in agreement with the clinical evaluation. Our results show the adopted treatment modified the serological profile of IgM antibodies and the PCR results, but not the IgG and IgA antibodies and that imaging is a good tool to follow-up @entity1 .

A Brazilian report using serological and molecular diagnosis to monitoring XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity6 :: ('MESH:D003920', 'Disease') :: ['Diabetic Retinopathy'] @entity17577 :: ('MESH:D014126', 'Disease') :: ['Ocular toxoplasmosis'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity941 :: ('MESH:D019793', 'Chemical') :: ['fluorescein'] @entity3204 :: ('5811', 'Species') :: ['Toxoplasma gondii']
@entity17577 :: (MESH:D014126,Disease) :: ['acute ocular toxoplasmosis']
