UNASSIGNED: @entity880 dehydrogenase (LDH) is a terminal enzyme in anaerobic glycolytic pathway. It widely exists in various organisms and is in charge of converting the glycolysis product @entity2388 to @entity880 . Most parasites, including @entity2440 , predominantly depend on glycolysis to provide energy. Bioinformatic analysis predicts that the LDHs from many species have more than one transmembrane region, suggesting that it may be a membrane protein. @entity2441 LDH (CsLDH) has been confirmed as a transmembrane protein mainly located in the tegument. The antibodies against CsLDH can inhibit the worm's energy metabolism, kill the worm, and may have the same effects on @entity1 @entity5 cells. In this study, we cloned and characterized @entity1 @entity3343 (HsLDHA), HsLDHB, and CsLDH. Semi-quantitative real-time RCP showed that HsLDHB only existed in hepatocarcinoma cell SMMC-7721. Confocal microscopy and Western blot experiments revealed that HsLDHB was localized in the plasma membrane of SMMC-7721 cells, and the antibodies against CsLDH could cross-react with it. This cross-reaction could inhibit the enzymatic activity of HsLDHB. The @entity5 cells co-cultured with anti-CsLDH sera showed a significant decrease in cell proliferation rate and increases in @entity8187 and reactive @entity26 species (ROS) levels. Therefore, anti-CsLDH antibodies can induce the apoptosis of @entity5 cells SMMC-7721 and may serve as a new tool to inhibit @entity5 .

Antibodies against @entity2440 LDH could cross-react with LDHB localizing on the plasma membrane of XXXX hepatocarcinoma cell SMMC-7721 and induce apoptosis.

@entity1 :: ('9606', 'Species') :: ['human'] @entity3343 :: ('3939', 'Gene') :: ['LDHA'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity8187 :: ('842', 'Gene') :: ['caspase 9'] @entity2441 :: ('143401', 'Species') :: ['C. sinensis'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactic acid', 'Lactate'] @entity2440 :: ('79923', 'Species') :: ['Clonorchis sinensis'] @entity2388 :: ('MESH:D019289', 'Chemical') :: ['pyruvic acid']
@entity1 :: (9606,Species) :: ['human']
