UNASSIGNED: Epigenetic changes play an important role in the pathogenesis of @entity11 and have the potential to become clinically useful biomarkers. The aim of this study was the evaluation of the profile of promoter methylation of 13 genes selected based on their anticipated diagnostic and/or prognostic value. Methylation-specific PCR (MSP) was used to assess the methylation status of @entity8724 , @entity11833 , @entity6756 , @entity14050 , @entity9288 ( @entity9288 ), @entity986 , @entity986 ( @entity986 ), @entity15893 , @entity10429 , @entity43289 , @entity16154 , @entity983 , and @entity4882 in a subset of 95 @entity11 of different grades. Additionally, the methylation status of @entity8724 and @entity16154 was analyzed using pyrosequencing (PSQ). The results revealed that the methylation index of individual @entity11 @entity1 correlates with World Health Organization (WHO) @entity5 grade and @entity1 's age. @entity15893 , @entity10429 , @entity43289 , and @entity8724 were most frequently methylated, whereas the @entity9288 -ARF-INK4A locus, @entity983 , @entity4882 , and @entity14050 were methylated to a lesser extent. @entity11833 , @entity6756 , and @entity16154 were unmethylated. @entity10429 methylation correlated with WHO @entity5 grade and @entity1 's age. PSQ confirmed significantly higher methylation levels of @entity8724 and @entity16154 as compared with normal, non-cancerous brain tissue. To conclude, DNA methylation of a whole panel of selected genes can serve as a tool for @entity146 prediction.

The methylation of a panel of genes differentiates low-grade from high-grade XXXX .

@entity1 :: ('9606', 'Species') :: ['patient', 'patients'] @entity6756 :: ('4292', 'Gene') :: ['hMLH1'] @entity11833 :: ('2067', 'Gene') :: ['ERCC1'] @entity43289 :: ('2627', 'Gene') :: ['GATA6'] @entity986 :: ('1029', 'Gene') :: ['CDKN2A', 'p16INK4A', 'p14ARF'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity9288 :: ('1030', 'Gene') :: ['CDKN2B', 'INK4B', 'p15INK4B'] @entity14050 :: ('472', 'Gene') :: ['ATM'] @entity146 :: ('MESH:D001523', 'Disease') :: ['glioma aggressiveness'] @entity11 :: ('MESH:D005910', 'Disease') :: ['gliomas', 'glioma'] @entity8724 :: ('4255', 'Gene') :: ['MGMT'] @entity983 :: ('5728', 'Gene') :: ['PTEN'] @entity16154 :: ('57447', 'Gene') :: ['NDRG2'] @entity15893 :: ('11186', 'Gene') :: ['RASSF1A'] @entity10429 :: ('864', 'Gene') :: ['RUNX3'] @entity4882 :: ('5915', 'Gene') :: ['RARb']
@entity11 :: (MESH:D005910,Disease) :: ['gliomas']
