UNASSIGNED: b-1,3-glucanosyltransferases play essential roles in cell wall biosynthesis in @entity375 . @entity11900 has six putative b-1,3-glucanosyltransferase genes. KlGAS1-1 and KlGAS1-2 are homologs of @entity375 gene @entity62623 . RT-qPCR indicated transcription level of KlGAS1-1 was significantly reduced while heterologous protein (thermostable xylanase B) secretion was enhanced during medium optimization. To evaluate if these two events were related, and to improve xylanase B secretion in @entity11900 , we constructed KlGAS1-1 and KlGAS1-2 single deletion strains and double deletion strain respectively. KlGAS1-1 gene deletion resulted in the highest xylanase B activity among the three mutants. Only the double deletion strain showed morphology similar to that of @entity62623 deletion mutant in @entity375 . The two single deletion strains differed in terms of cell wall thickness and xylanase B secretion. Transcription levels of b-1,3-glucanosyltransferase genes and genes related to protein secretion and transport were assayed. The b-1,3-glucanosyltransferase genes displayed transcription complementation in the cell wall synthesis process. KlGAS1-1 and KlGAS1-2 affected transcription levels of secretion- and transport-related genes. Differences in protein secretion ratio among the three deletion strains were associated with changes of transcription levels of secretion- and transport-related genes. Our findings indicate that KlGAS1-1 deletion is an effective tool for enhancing industrial-scale heterologous protein secretion in @entity11900 .

Characterization of two @entity62623 genes and their effects on expression and secretion of heterologous protein xylanase B in XXXX .

@entity11900 :: ('28985', 'Species') :: ['Kluyveromyces lactis', 'K. lactis'] @entity375 :: ('4932', 'Species') :: ['yeast', 'Saccharomyces cerevisiae'] @entity62623 :: ('855355(Tax:4932)', 'Gene') :: ['GAS1']
@entity11900 :: (28985,Species) :: ['Kluyveromyces lactis']
