Spontaneous rhythmic activity is a ubiquitous feature of developing neural structures that has been shown to be essential for the establishment of functional CNS connectivity. However, the primordial origin of these rhythms remains unknown. Here, we describe two types of rhythmic activity in distinct parts of the developing CNS isolated ex vivo on microelectrode arrays, the expression of which was found to be strictly dependent upon the movement of the artificial @entity2304 (aCSF) flowing over the inner wall of the ventricles or over the outer surface of the CNS. First, whole embryonic @entity19 hindbrain-spinal cord preparations (stages E12.5-E15.5) rhythmically expressed waves of activity originating in the hindbrain and propagating in the spinal cord. Interestingly enough, the frequency of this rhythm was completely determined by the speed of the aCSF flow. In particular, at all stages considered, hindbrain activity was abolished when the perfusion was stopped. Immature rhythmic activity was also recorded in the isolated newborn (P0-P8) @entity19 cortex under normal aCSF perfusion. Again, this rhythm was abolished when the perfusion flow was stopped. In both structures, this phenomenon was not due to changes in temperature, @entity26 level, or pH of the bath, but to the movement itself of the aCSF. These observations challenge the so-called "spontaneous" nature of rhythmic activity in immature neural networks and suggest that the movement of @entity2304 in the ventricles and around the brain in vivo may mechanically drive rhythmogenesis in the developing CNS.

Artificial XXXX motion ensures rhythmic activity in the developing CNS ex vivo: a mechanical source of rhythmogenesis?

@entity2304 :: ('12981(Tax:10090)', 'Gene') :: ['CSF'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity19 :: ('10090', 'Species') :: ['mouse']
@entity2304 :: (12981(Tax:10090),Gene) :: ['CSF']
