Emergency Preservation and Resuscitation (EPR) represents a novel approach to treat exsanguination @entity1167 ( @entity1167 ) victims, using an aortic flush to induce @entity2332 during @entity1167 , followed by delayed resuscitation with cardiopulmonary bypass (CPB). The status of the blood-brain barrier (BBB) integrity after prolonged hypothermic @entity1167 is unclear. The objective of this study was to assess BBB permeability in two EPR models in @entity35 , associated with poor outcome. @entity35 subjected to @entity131 ( @entity131 ) and na  ve @entity35 served as positive and negative controls, respectively. HYPOTHESIS: The BBB will be disrupted after @entity131 , but intact after prolonged hypothermic @entity1167 . METHODS: Four groups were studied: (1) EPR-IC (ice cold)-75 min @entity1167 at 15 degrees C; (2) EPR-RT (room temperature)-20 min @entity1167 at 28 degrees C; (3) @entity131 ; (4) sham. @entity35 in EPR groups were subjected to rapid @entity548 , followed by @entity1167 . @entity35 in the @entity131 group had a controlled cortical impact to the left hemisphere. Na  ves were subjected to the same anesthesia and surgery. 1h after insult, @entity35 were injected with @entity23045 (EB), a marker of BBB permeability for albumin. @entity35 were sacrificed after 5h and EB absorbance was quantified in brain samples. RESULTS: @entity131 produced an approximately 10-fold increase in EB absorbance in the left (injured) hemisphere vs. left hemisphere for all other groups (p=0.001). In contrast, EB absorbance in either EPR group did not differ from sham. CONCLUSION: BBB integrity to albumin is not disrupted early after resuscitation from prolonged @entity1167 treated with EPR. Neuroprotective adjuncts to @entity2332 in this setting should focus on agents that penetrate the BBB. These findings also have implications for deep hypothermic @entity1167 .

Blood-brain barrier integrity in a XXXX model of emergency preservation and resuscitation.

@entity131 :: ('MESH:D001930', 'Disease') :: ['TBI', 'traumatic brain injury'] @entity548 :: ('MESH:D006470', 'Disease') :: ['hemorrhage'] @entity23045 :: ('MESH:D005070', 'Chemical') :: ['Evans Blue'] @entity1167 :: ('MESH:D006323', 'Disease') :: ['cardiac arrest', 'circulatory arrest', 'CA'] @entity35 :: ('10116', 'Species') :: ['Rats', 'rats'] @entity2332 :: ('MESH:D007035', 'Disease') :: ['hypothermia']
@entity35 :: (10116,Species) :: ['rat']
