@entity2550 protein 60 (Ye- @entity11217 ) has recently been found to be a dominant @entity404 and @entity405 T cell Ag in Yersinia-triggered @entity625 . The nature of this response with respect to the epitopes recognized and functional characteristics of the T cells is largely unknown. @entity404 + T cell clones specific for Ye- @entity11217 were raised from synovial fluid mononuclear cells from a @entity1 with Yersinia-triggered @entity625 . and their specificity was determined using three recombinant Ye- @entity11217 fragments, overlapping 18-mer synthetic peptides as well as truncated peptides. Functional characteristics were assessed by cytokine secretion analysis in culture supernatants after specific antigenic stimulation. @entity535 positions relevant for T cell activation were detected by single @entity493 substitutions within the epitopes. Fragment II comprising @entity535 sequence 182-371 was recognized by the majority of clones. All these clones were specific for peptide 319-342. Th1 clones and @entity688 -secreting clones occurred in parallel, sometimes with the same fine specificity. The 12-mer core epitope 322-333 is a degenerate MHC binder and is presented to some T cell clones in a "promiscuous" manner. This epitope is almost identical with a B27-restricted CTL epitope of Ye- @entity11217 . Cross-reactivity of Ye- @entity11217 -specific T cell clones with self- @entity11217 was not observed. In conclusion, an interesting Ye- @entity11217 T cell epitope has been identified and characterized. It remains to be determined whether this epitope is also relevant in other @entity625 @entity1 .

Multispecific @entity404 + T cell response to a single 12-mer epitope of the immunodominant heat-shock protein 60 of Yersinia enterocolitica in Yersinia-triggered XXXX : overlap with the B27-restricted @entity405 epitope, functional properties, and epitope presentation by multiple DR alleles.

@entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity404 :: ('920', 'Gene') :: ['CD4'] @entity625 :: ('MESH:D001168', 'Disease') :: ['reactive arthritis'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid', 'Amino acid'] @entity2550 :: ('MESH:D015009', 'Disease') :: ['Yersinia heat-shock'] @entity688 :: ('3586', 'Gene') :: ['IL-10'] @entity493 :: ('CHEBI:16449', 'Chemical') :: ['alanine'] @entity11217 :: ('3329', 'Gene') :: ['hsp60'] @entity405 :: ('925', 'Gene') :: ['CD8']
@entity625 :: (MESH:D001168,Disease) :: ['reactive arthritis']
