In this study, we explored the mechanisms by which the angiotensin converting enzyme inhibitor (ACEI), @entity4688 , and the Ang II receptor blocker (ARB), @entity9886 suppress oxidative stress and @entity14185 activation-induced @entity32 responses in aged @entity35 kidney. The experimentations were carried out utilizing aged (24-month-old) Brown Norway Fischer 344 (F1) male @entity35 which were randomized into 3 groups and administered @entity4688 (40 mg/kg), @entity9886 (30 mg/kg) or placebo for 6 months (daily p.o.). The level of reactive species (RS), @entity6710 ( @entity29101 ), @entity2197 / @entity9492 and lipid peroxidation were measured. The activity of the pro- @entity32 transcription factor @entity14185 , and gene expression of proteins in upstream signaling cascades were measured by electro-mobility shift assay (EMSA) and Western blotting. @entity4688 and @entity9886 differentially attenuated redox imbalance and the redox-sensitive transcription factor, the @entity14185 pathway. Furthermore, stimulation of the @entity14185 activation pathway by phosphorylation of @entity12149 was attenuated by both compounds. Moreover, mediation of phosphorylation of @entity12149 by phosphorylation of IkB kinase ab (IKKab) and mitogen- and stress-activated protein kinase-1 ( @entity70092 ), were also inhibited by @entity4688 and @entity9886 . Finally, both compounds also lowered expression of @entity14185 -dependent @entity32 genes, such as @entity4645 ( @entity4645 ), and @entity1506 ( @entity1506 ). Only @entity9886 lowered levels of @entity12032 (5-LOX). These findings indicate that @entity4688 and @entity9886 differentially suppress @entity32 responses via inhibition of oxidative stress-induced @entity14185 activation in aged @entity35 kidney.

Inhibition of @entity14185 -induced XXXX responses by angiotensin II antagonists in aged @entity35 kidney.

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammatory'] @entity4688 :: ('MESH:D004656', 'Chemical') :: ['enalapril', 'Enalapril'] @entity9492 :: ('MESH:D019803', 'Chemical') :: ['GSSG'] @entity70092 :: ('314384(Tax:10116)', 'Gene') :: ['MSK-1'] @entity12149 :: ('25716(Tax:10116)', 'Gene') :: ['p65'] @entity1506 :: ('24599(Tax:10116)', 'Gene') :: ['inducible NO synthase', 'iNOS'] @entity12032 :: ('25290(Tax:10116)', 'Gene') :: ['5-lipoxygenase'] @entity9886 :: ('MESH:D019808', 'Chemical') :: ['losartan'] @entity2197 :: ('CHEBI:16856', 'Chemical') :: ['GSH'] @entity29101 :: ('CHEBI:33258', 'Chemical') :: ['ONOO(-)'] @entity14185 :: ('309165(Tax:10116)', 'Gene') :: ['NF-kB'] @entity6710 :: ('MESH:D030421', 'Chemical') :: ['peroxynitrite'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity4645 :: ('29527(Tax:10116)', 'Gene') :: ['COX-2', 'cyclooxygenase-2']
@entity32 :: (MESH:D007249,Disease) :: ['inflammatory']
