The use of human monoclonal antibodies ( @entity9623 ) in the detection and treatment of @entity1 @entity5 has been limited by the apparent scarcity of @entity9623 to @entity5 cell surface antigens. Using peripheral blood lymphocytes from autologous @entity5 -immunized @entity1 , we isolated 36 @entity9623 that react to sections of @entity14 . Twenty of these @entity1 @entity9623 appear to be directed against cell surface antigens. Two-thirds of the @entity1 @entity9623 -producing cell lines were diploid @entity1 B-cells rather than @entity1 - @entity19 heterohybridomas. Direct antibody-binding assays performed with the @entity9623 indicated that they recognized antigenic determinants preferentially expressed on @entity5 cells. Experiments with paired specimens of air-dried, dissociated @entity694 cells and normal colonic mucosa cells suggested that the @entity9623 bound significantly more to the cell surfaces of @entity5 cells than to the surfaces of normal colonic mucosa cells. Similarly, tests with a panel of cryostat sections of paired @entity694 and normal colonic mucosa showed that @entity9623 bound to the @entity5 cells and not to the normal colonic mucosa. None of the @entity9623 bound to cells from frozen sections of normal breast, stomach, liver, skeletal muscle, or skin. Furthermore, the @entity1 @entity9623 did not react with carcinoembryonic antigen and human erythrocyte antigens as measured by various techniques. Our data also demonstrated that these transformed B-cells and hybridomas were stable producers of @entity1 @entity9623 . Thus, our studies show that these @entity5 -specific @entity1 @entity9623 may have the specificity and stability necessary for in vivo evaluation of their use in the detection and treatment of @entity5 .

Generation of @entity5 cell-reactive human monoclonal antibodies using peripheral blood lymphocytes from actively immunized @entity14 XXXX .

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal carcinoma'] @entity9623 :: ('CHEBI:34342', 'Chemical') :: ['MCA'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon tumor']
@entity1 :: (9606,Species) :: ['patients']
