OBJECTIVE: To investigate whether @entity69243 suppresses osteogenic differentiation of @entity19 mesenchymal stem cells (C3H10T1/2) by targeting @entity21602 , a predicted target in @entity6619 . METHODS: @entity21602 3'-UTR-luciferase vector (pmiR-RB-REPORT) was constructed and dual-luciferase reporter gene assay was employed to examine the effect of @entity69243 on @entity21602 3'-UTR-luciferase activity to identify whether @entity21602 was the target gene of @entity69243 . @entity69243 mimics (group M), @entity69243 inhibitor (group In), @entity69243 mimics negative control (group MC), and @entity69243 inhibitor negative control (group InC) were transfected into the C3H10T1/2 cells, respectively, and followed by induction of osteogenic differentiation. After 48 hours, the real-time fluorescent quantitative PCR (qRT-PCR) and Western blot assays were performed to detect the relative expressions of @entity21602 mRNA and protein. At 14 days, to realize the regulation role of @entity69243 in osteogenic differentiation, the extracellular @entity460 deposition during the osteogenesis of C3H10T1/2 cells was tested by @entity622 staining. RESULTS: Dual-luciferase reporter gene assay showed that @entity69243 could combine with @entity21602 mRNA 3'-UTR specificity, and inhibited its luciferase activity (P < 0.05). After 48 hours, no significant difference was shown in the relative expression of @entity21602 mRNA between group M and group MC as well as between group In and group InC by qRT-PCR assay (P > 0.05); however, the results of Western blot assay showed that the relative expression of @entity21602 protein was significantly decreased in group M and increased in group In when compared with groups MC and InC (P < 0.05). At 14 days after osteogenic induction, @entity622 staining showed that the extracellular @entity460 deposition of group M was obviously less than that of group MC, and it was obviously more in group In than in group InC. CONCLUSION: @entity21602 may be the target gene of @entity69243 . And @entity31462 -5p can suppress osteogenic differentiation of C3H10T1/2 cells by directly targeting @entity21602 .

[ @entity31462 -5P SUPPRESSES OSTEOGENIC DIFFERENTIATION OF XXXX C3H10T1/2 CELLS BY TARGETING @entity21602 ].

@entity21602 :: ('17129(Tax:10090)', 'Gene') :: ['Smad5'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity69243 :: ('104795667', 'Gene') :: ['miR-93-5p'] @entity622 :: ('MESH:C010078', 'Chemical') :: ['Alizarin red'] @entity31462 :: ('723968(Tax:10090)', 'Gene') :: ['miR-93'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity6619 :: ('MESH:D012825', 'Chemical') :: ['silicon']
@entity19 :: (10090,Species) :: ['MOUSE']
