The direct intraparenchymal administration of oncolytic viral vectors by @entity14782 ( @entity14782 ) represents a promising new treatment strategy for @entity11 . However, there is no evidence to suggest that oncolytic viruses as large as @entity3307 ) can be administered by @entity14782 , as this has not been systematically examined in an animal model. In this study, the administration of a herpes simplex viral vector, @entity3307 , has been evaluated in detail in the gray and white matter of both @entity35 and @entity1707 models, using high flow-rate infusions, co-infusing heparin or preinfusing the tissue with an isotonic albumin solution. @entity35 @entity3307 infusions at both slow (0.5  l min(-1)) and high infusion rates (2.5  l min(-1)) led to extensive tissue damage and negligible cell transduction. Co-infusion with heparin led to extensive @entity548 . Preinfusion of tissue with an isotonic albumin solution facilitated widespread vector distribution and cell transduction in white matter only. Using this approach in @entity1707 brain led to widespread vector distribution with extensive transduction of astrocytes and activated microglia. In @entity35 brain, enhanced green fluorescent protein expression peaked 48 h after vector administration and was associated with a vigorous immune response. These findings indicate that direct infusions of @entity3307 -based viral vectors into the brain lead to minimal vector distribution, negligible cell transduction and extensive damage. Tissue preinfusion with an isotonic solution prior to vector administration represents an effective technique for achieving widespread @entity3307 distribution.

Evaluation and optimization of the administration of a selectively replicating herpes simplex viral vector to the brain by XXXX .

@entity1707 :: ('9823', 'Species') :: ['pig'] @entity548 :: ('MESH:D006470', 'Disease') :: ['hemorrhage'] @entity14782 :: ('MESH:C564835', 'Disease') :: ['convection-enhanced delivery', 'CED'] @entity11 :: ('MESH:D005910', 'Disease') :: ['malignant gliomas'] @entity35 :: ('10116', 'Species') :: ['rat', 'Rat'] @entity3307 :: ('10298', 'Species') :: ['herpes simplex virus-1 (HSV-1', 'HSV1', 'HSV-1']
@entity14782 :: (MESH:C564835,Disease) :: ['convection-enhanced delivery']
