A standardized @entity515 extract from Humulus lupulus L. (hop extract) was investigated for its selective @entity48902 inhibitory properties. An in vitro model of @entity32 using lipopolysaccharide (LPS)-stimulated @entity1 peripheral blood mononuclear cells (PBMC) was used as a model to investigate the effect of hop extract on @entity1421 production. @entity48902 selective inhibition by the hop extract was investigated in a @entity16538 whole blood assay (WBA) and a @entity10178 WBA. To evaluate the in vivo activity of hop extract, it was administered orally to C57BL/6 @entity19 in which @entity32 of the right joint was induced by injecting zymosan intra-articularly. Ex vivo @entity1421 production of LPS-stimulated blood cells was determined. Also, the effect of hop extract on healthy and arthritic cartilage was investigated as well as effects on inflammatory joint @entity4 . Hop extract inhibited @entity1421 production by LPS-stimulated PBMC without compromising the metabolic activity of these cells. Furthermore, hop extract showed a decline in @entity1421 production in the @entity10178 whole blood assay (WBA) with an IC(50) of 20.4 microg/mL, while in the @entity16538 WBA no inhibition of @entity1421 production was observed. This indicates a @entity10178 selective inhibition. The @entity16538 inhibitor @entity16539 inhibited @entity1421 production in the @entity16538 WBA but not in the @entity10178 WBA. At 2 microM, @entity12698 inhibited @entity1421 production in the @entity10178 WBA by 92 % and in the @entity16538 WBA by 50 %. When hop extract was administered orally to C57BL/6 @entity19 in which joint @entity32 was induced with zymosan, @entity1421 production in ex vivo LPS-stimulated whole blood was significantly decreased by 24 %, suggesting that hop extract becomes bioavailable. Furthermore, oral administration of hop extract showed no negative or positive effects on healthy cartilage proteoglycan synthesis, or on zymosan-induced arthritic cartilage proteoglycan synthesis. However, no effect of oral administration of 1.25 mg hop extract daily was observed on joint @entity4 . In conclusion, this standardized @entity515 extract of Humulus lupulus could be a useful agent for intervention strategies targeting @entity32 and/or inflammatory @entity158 .

Selective inhibition of @entity10178 by a standardized @entity515 extract of Humulus lupulus in vitro and its activity in a XXXX model of zymosan-induced arthritis.

@entity1 :: ('9606', 'Species') :: ['human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammatory disorders', 'inflammation'] @entity16538 :: ('17708(Tax:10090)', 'Gene') :: ['COX-1'] @entity10178 :: ('17709(Tax:10090)', 'Gene') :: ['COX-2'] @entity12698 :: ('MESH:C105934', 'Chemical') :: ['celecoxib'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity1421 :: ('MESH:D015232', 'Chemical') :: ['PGE(2)'] @entity4 :: ('MESH:D004487', 'Disease') :: ['swelling'] @entity515 :: ('CHEBI:29334', 'Chemical') :: ['CO(2)'] @entity16539 :: ('MESH:C115461', 'Chemical') :: ['SC-560'] @entity158 :: ('MESH:D010146', 'Disease') :: ['pain'] @entity48902 :: ('17708;17709', 'Gene') :: ['COX-1/2']
@entity19 :: (10090,Species) :: ['mouse']
