AIM: The detection of circulating @entity5 cells in the bloodstream before and after surgical manipulation, and the qualitative detection of potential shedding of @entity5 cells during surgical manipulation of @entity1 with @entity2184 . METHODS: 202 @entity1 treated for a newly diagnosed @entity2184 were included in the study. Blood samples were acquired 24   h before and 30   min after the basic surgical steps. Detection of potential circulating @entity320 cells was extrapolated from the presence of @entity11442 and @entity3059 / @entity3059 transcripts by reverse transcription PCR. RESULTS: Based on the measurement of @entity11442 transcripts, as a result of the first and second surgical manipulation there were three and zero transitions from negative to positive respectively, while there were two and one transitions from positive to negative, respectively. According to @entity3059 / @entity3059 transcripts, there were 19 and 5 transitions from negative to positive respectively, and 15 and 2 transitions from positive to negative, respectively. No statistically significant differences were documented, concerning the presence of circulating @entity5 cells in the blood samples acquired before and after the first surgical manipulation or the second one. CONCLUSION: The change in the percentage of @entity1 with detected @entity5 cells in their bloodstream was not statistically significant. The frequent shifts from negative to positive samples as well as from positive to negative samples comparing preoperative to postoperative samples indicates discontinuous shedding or variation due to measurements close to the threshold of detection. As a conclusion, the surgical manipulation does not seem to have a measurable contribution to the spread of @entity320 cells in the bloodstream.

@entity5 cells in the bloodstream of @entity1 with XXXX : influence of surgical manipulation on the dissemination of @entity5 cells in the bloodstream.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumour'] @entity2184 :: ('MESH:C536494', 'Disease') :: ['uveal melanoma'] @entity3059 :: ('2315', 'Gene') :: ['Mart1', 'MelanA'] @entity11442 :: ('7299', 'Gene') :: ['tyrosinase'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma']
@entity2184 :: (MESH:C536494,Disease) :: ['uveal melanoma']
