INTRODUCTION: @entity1073 ( @entity1073 ) is a clinicopathological entity characterized by an aggressive clinical course, morphological features, and overexpression of @entity4910 due to juxtaposition of the @entity4910 locus (and @entity4910 gene coding for the @entity4910 ) to the @entity7513 gene. This phenomenon is caused by t(11;14)(q13;q32). The morphological diagnosis of @entity1073 may pose difficulties. Ancillary methods are available to support the diagnosis. @entity1 AND METHODS: We studied a group of 32 @entity1 with @entity1073 ; 24 @entity1 and 8 @entity1 . The median age at the diagnosis was 64 years. We characterized the investigated group by histology, and to analyze the immunohistochemical (IHC) profile we used a panel of antibodies including anti- @entity4910 . Polymerase chain reaction (PCR) was used to detect the rearrangement of @entity4910 / @entity7513 in 26 cases (in 11 @entity1 , the DNA was isolated from frozen tissues or from nucleated cells of bone-marrow aspirate or peripheral blood, in 15 @entity1 we utilized paraffin-embedded material). Dual color fluorescence in situ hybridization (FISH) on interphase nuclei detecting the t(11;14)(q13;q32) was applied in all 32 cases. RESULTS: @entity4910 IHC was positive in 29 of 30 cases tested (97%). In six, the result was weak and difficult to rely on to support the diagnosis. PCR revealed the fusion gene in 14 of the 26 cases (54%). The best yield was obtained from fresh and frozen samples (8 of 11 positive). Using FISH, we identified the translocation in all 32 @entity1 , the findings being easily interpretable in 29 @entity1 . In three cases, the intensity of red and green signals was weaker and difficult to read though the co-hybridized signals were identified. The classical pattern of the translocation was observed in 26 @entity1 , while in 3 we found variant patterns suggesting a loss of the V segment of the @entity7513 gene (2x) and a shift in the breakpoint region at chromosome 11 (1x). CONCLUSION: The diagnosis of @entity1073 should be supported by a complex laboratory approach. Interphase FISH seems a useful complementary method to morphology and IHC. It is applicable to various tissues and cells prepared as tissue imprints or histological sections.

XXXX : improved diagnostics using a combined approach of immunohistochemistry and identification of t(11;14)(q13;q32) by polymerase chain reaction and fluorescence in situ hybridization.

@entity7513 :: ('3492', 'Gene') :: ['IgH'] @entity1 :: ('9606', 'Species') :: ['men', 'women', 'patients', 'PATIENTS'] @entity4910 :: ('595', 'Gene') :: ['cyclin D1', 'Cyclin D1', 'bcl-1', 'CCND1'] @entity1073 :: ('MESH:D020522', 'Disease') :: ['Mantle cell lymphoma', 'MCL']
@entity1073 :: (MESH:D020522,Disease) :: ['Mantle cell lymphoma']
