CONTEXT: @entity1892 ( @entity1892 ) is the most common cause of early-onset @entity295 in developed countries, and determination of the @entity1892 colonization status in pregnant @entity1 near term is essential for the provision of prophylactic measures to prevent early-onset disease. OBJECTIVES: To determine if @entity1892 recovery rates and/or result turnaround times for vaginal or combined vaginal/rectal swab specimens from pregnant @entity1 near term are enhanced if swabs are inoculated initially onto selective versus nonselective agar media, in addition to the standard Centers for Disease Control and Prevention method. DESIGN: Prospective laboratory analysis. SETTING: Urban health region/centralized diagnostic microbiology laboratory. @entity1 : Pregnant @entity1 presenting for routine obstetrical care and collection of vaginal or combined vaginal/rectal swab specimens for @entity1892 testing at 35 to 37 weeks' gestation. INTERVENTION: Culture of specimens directly onto selective (5% @entity742 blood with @entity3273 and @entity802 ) or nonselective (5% @entity742 blood) agar media, in addition to @entity18282 broth enrichment and terminal subculture. MAIN OUTCOME MEASURES: @entity1892 recovery rate and culture result turnaround time. RESULTS: A total of 639 specimens were tested, with 128 (20%) positive for @entity1892 . Sixty-three isolates were recovered on direct agar media at 24 hours, of which 16 (12.5%) were isolated on selective plates only. An additional 38 isolates were recovered at 48 hours from direct plates. Twenty-seven (21.1%) isolates that failed to grow on direct plates were recovered from the @entity18282 broth subculture only. Three (2.3%) isolates not recovered from @entity18282 broths were detected at 48 hours on the direct selective (2 isolates) and nonselective (1 isolate) agar plates. A 24-hour result turnaround time was achieved for 63 (49.2%) and 47 (36.7%) of the 128 culture-positive specimens for direct selective and nonselective plates, respectively (chi2 = 76.63, P <.001). CONCLUSIONS: Use of direct selective agar media, in addition to @entity18282 broth enrichment, for the determination of the @entity1892 colonization status in pregnant @entity1 near term results in decreased turnaround time for reporting positive results.

Comparison of direct selective versus nonselective agar media plus XXXX broth enrichment for determination of group B streptococcus colonization status in pregnant @entity1 .

@entity1 :: ('9606', 'Species') :: ['women', 'patients', 'PATIENTS'] @entity18282 :: ('10611', 'Gene') :: ['LIM'] @entity295 :: ('MESH:D018805', 'Disease') :: ['neonatal sepsis'] @entity3273 :: ('MESH:D003091', 'Chemical') :: ['colistin'] @entity742 :: ('9940', 'Species') :: ['sheep'] @entity1892 :: ('MESH:D016393', 'Disease') :: ['GBS', 'Group B streptococcus'] @entity802 :: ('MESH:D009268', 'Chemical') :: ['nalidixic acid']
@entity18282 :: (10611,Gene) :: ['LIM']
