PURPOSE: To evaluate the roles of @entity6510 cannabinoid receptors in cellular functions of trabecular meshwork (TM) cells, including cell migration, adhesion, morphology and cytoskeleton changes. METHODS: @entity60606 , a selective @entity6510 receptor agonist, and @entity351 , a selective @entity6510 receptor antagonist, were used to characterize the cellular functions of cultured porcine TM cells. Fluorescence assisted transmigration invasion and motility assays (FATIMA) were conducted to study TM cell migration using soluble fibronectin as a chemoattractant. Wound healing assays were used to further study TM cell migration. Standard cell adhesion assays of TM cells were performed on fibronectin-coated plates. In morphological studies, @entity21112 -labeled @entity6286 staining was used to examine actin filaments, and immunocytochemistry using anti-paxillin antibodies was used to detect focal adhesions. RESULTS: In cell migration assays, @entity6510 agonist @entity60606 at nanomolar ranges led to a concentration-dependent inhibition of migration of TM cells toward soluble fibronectin. @entity6510 antagonist @entity351 antagonized @entity60606 -induced inhibition of migration of TM cells. In addition, @entity60606 caused a delay in wound healing of confluent trabecular meshwork monolayers and this effect of @entity60606 was antagonized by @entity351 . In cell adhesion assays, @entity60606 treatment led to a moderate, but significant decrease of adhesion of TM cells to fibronectin-coated surface. This effect of @entity60606 was concentration-dependent, and was antagonized by @entity351 . In morphological studies, @entity60606 treatment caused rounding of TM cells in contrast to well-spread control TM cells. In addition, there was a reduction and fragmentation of actin stress fibers stained with @entity21112 -labeled @entity6286 and a decrease of focal adhesions detected with an anti-paxillin antibody. CONCLUSIONS: @entity60606 modulates the migration, adhesion, morphology, and actin cytoskeleton of TM cells. These effects of @entity60606 are mediated through TM cell @entity6510 cannabinoid receptors.

XXXX cannabinoid receptor-mediated changes of trabecular meshwork cellular properties.

@entity6510 :: ('1268', 'Gene') :: ['CB1'] @entity60606 :: ('MESH:C424442', 'Chemical') :: ['Noladin ether', 'noladin ether'] @entity6286 :: ('MESH:D010590', 'Chemical') :: ['phalloidin'] @entity21112 :: ('CHEBI:52661', 'Chemical') :: ['Alexafluor 488'] @entity351 :: ('MESH:C089032', 'Chemical') :: ['SR141716A']
@entity6510 :: (1268,Gene) :: ['CB1']
