UNASSIGNED: @entity957 ( @entity957 ) is a type of head and @entity5 with a high mortality rate. @entity2345 ( @entity2345 ) is the pre-cancerous lesion of @entity957 , whose molecular mechanisms in @entity957 tumorigenesis remain largely unclear. Activation of the Wnt/b-catenin signaling pathway plays an important role in oral mucous carcinogenesis, although rare mutations of Wnt signaling molecules are found in @entity957 , suggesting an epigenetic mechanism mediating aberrant Wnt/b  -catenin signaling in @entity957 . @entity21124 ( @entity21124 ) is an Wnt antagonist, and its downregulation and methylation have been reported in a number of @entity5 . However, the expression and methylation of @entity21124 in the development of @entity2345 have yet to be reported. In the present study, we investigated the @entity21124 expression level by immuno-histochemical staining and semi  -quantitative RT-PCR in normal oral, @entity2345 and @entity957 tissues, as well as the methylation status by methylation-specific PCR and @entity9736 genomic sequencing. The results showed that @entity21124 was readily expressed in normal oral mucous tissues, but decreased gradually in @entity2345 early, moderately advanced and advanced tissues, and was less expressed in @entity957 tissues. Moreover, @entity21124 was able to translocate from the nuclear to cytoplasm in @entity2345 and @entity957 tissues. Furthermore, @entity21124 was frequently methylated in @entity957 cases with betel quid chewing habit, but not in normal oral mucous and different stages of @entity2345 tissues, suggesting @entity21124 methylation is tumor-specific in the development of @entity2345 . Thus, the results demonstrated that @entity21124 is frequently downregulated or silenced by promoter methylation in the carcinogenesis of @entity2345 , which serves as a potential epigenetic biomarker for the early detection of @entity957 .

Expression and promoter methylation of @entity21124 in the development of XXXX .

@entity2345 :: ('MESH:D009914', 'Disease') :: ['OSF', 'Oral submucous fibrosis'] @entity5 :: ('MESH:D009369', 'Disease') :: ['neck malignancy', 'malignancies'] @entity957 :: ('MESH:D002294', 'Disease') :: ['OSCC', 'Oral squamous cell carcinoma'] @entity9736 :: ('MESH:C042345', 'Chemical') :: ['bisulfite'] @entity21124 :: ('11197', 'Gene') :: ['WIF1', 'Wnt inhibitory factor-1']
@entity2345 :: (MESH:D009914,Disease) :: ['oral submucous fibrosis']
