BACKGROUND _ AIMS: Hepatic stellate cells play an important role in liver fibrogenesis, and @entity99 may be involved in the termination of this response. METHODS: Molecular mechanisms of hepatic stellate cell killing were studied in hepatic stellate cell/Kupffer cell cocultures. RESULTS: Lipopolysaccharide stimulation of hepatic stellate cell/Kupffer cell cocultures, but not of hepatic stellate cell monocultures, induced profound alterations of hepatic stellate cell morphology and @entity99 . Kupffer cell-induced hepatic stellate cell killing required hepatic stellate cell/Kupffer cell contacts and was prevented by @entity619 , @entity1421 , @entity5 @entity39 factor-related apoptosis-inducing ligand ( @entity2205 ) receptor 2 antagonists, and down-regulation of receptor-interacting protein, but not by antioxidants, @entity5 @entity39 factor receptor, or @entity1197 antagonists. Hepatic stellate cell @entity204 was characterized by activation of @entity15418 , terminal deoxynucleotidyl transferase-mediated @entity9342 nick-end labeling negativity, lack of gross @entity460 overload, and @entity2205 trafficking to the plasma membrane. Inhibition of @entity8187 , but not of caspases 3, 8, or 10, prevented @entity99 . Lipopolysaccharide induced a @entity619 - and @entity1421 -sensitive expression of @entity2205 in Kupffer cells. @entity3048 , @entity1182 ( @entity8186 -inhibitory protein), and receptor-interacting protein were up-regulated during hepatic stellate cell transformation; however, @entity2205 addition did not induce @entity99 . Hepatic stellate cell susceptibility toward Kupffer cell-induced @entity204 paralleled receptor-interacting protein and @entity2205 -receptor expression levels. CONCLUSIONS: Activated Kupffer cell can effectively kill hepatic stellate cell by a @entity8187 - and receptor-interacting protein-dependent mechanism, possibly involving @entity2205 . The data may suggest a novel form of @entity99 .

XXXX -dependent killing of hepatic stellate cells by activated Kupffer cells.

@entity1182 :: ('8837', 'Gene') :: ['FLIP'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity1421 :: ('MESH:D015232', 'Chemical') :: ['prostaglandin E(2)'] @entity2205 :: ('8743', 'Gene') :: ['TRAIL'] @entity1197 :: ('355', 'Gene') :: ['CD95'] @entity3048 :: ('8797', 'Gene') :: ['TRAIL receptors 1 and 2'] @entity8186 :: ('841', 'Gene') :: ['caspase 8'] @entity8187 :: ('842', 'Gene') :: ['caspase 9'] @entity619 :: ('MESH:D003907', 'Chemical') :: ['dexamethasone'] @entity15418 :: ('836;841;842', 'Gene') :: ['caspases 3, 8, and 9'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity99 :: ('MESH:D056486', 'Disease') :: ['hepatic stellate cell death'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death'] @entity9342 :: ('MESH:C027078', 'Chemical') :: ['deoxyuridine triphosphate']
@entity8187 :: (842,Gene) :: ['Caspase 9']
