@entity2227 dehydrogenase (GDH) has been encapsulated into @entity19 erythrocytes by a @entity2421 dialysis/isotonic resealing method. Although a low GDH entrapment yield was achieved (3.8%), this percentage appeared sufficient enough to metabolize high quantities of @entity3265 . Carrier cell recovery yield was 56%. Due to the decrease in cell volume and haemoglobin content, constant mean cell haemoglobin concentration (MCHC) values were obtained. The osmotic fragility curves (OFC) indicated that dialyzed/resealed-RBCs are more resistant to @entity2421 haemolysis than native-RBCs. The successful in vitro @entity3265 degradation by GDH-RBCs was reflected in its total disappearance from the incubation medium at around 48 h. In contrast, initial @entity3265 levels were not affected during the incubation in the presence of native-RBCs and remained constant. Two different methods were used for the preparation of hyperammonaemic @entity19 model. Since the intraperitoneal (i.p.) administration of @entity6190 produced high @entity3265 levels that lasted only a few minutes, the i.p. administration of urease was chosen, given that it generated elevated @entity3265 levels for longer periods of time. Hyperammonaemic @entity19 quickly removed high levels of circulating @entity3265 in the presence of GDH-RBCs, whereas in the presence of native-RBCs @entity3265 was slowly metabolized. These results suggest that loaded GDH-erythrocytes can be used as a potential carrier systems for the in vivo removal of high levels of @entity3265 from blood.

In vitro and in vivo study of @entity2227 dehydrogenase encapsulated into XXXX erythrocytes by a @entity2421 dialysis procedure.

@entity2227 :: ('CHEBI:29987', 'Chemical') :: ['Glutamate'] @entity3265 :: ('MESH:D000641', 'Chemical') :: ['ammonia'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity6190 :: ('MESH:C018824', 'Chemical') :: ['ammonium acetate'] @entity2421 :: ('MESH:D009123', 'Disease') :: ['hypotonic']
@entity19 :: (10090,Species) :: ['mouse']
