BACKGROUND: Moderate red wine consumption is inversely associated with @entity63 , and both red wine and purple grape juice ( @entity70338 ) contain @entity3088 with antioxidant and antiplatelet properties believed to be protective against cardiovascular events. Acute @entity299 are also associated with decreased platelet-derived @entity842 (NO) release. In this study, the effects of @entity70338 and @entity70338 -derived @entity3088 on platelet function and platelet NO production were determined. METHODS AND RESULTS: Incubation of platelets with dilute @entity70338 led to inhibition of aggregation, enhanced release of platelet-derived NO, and decreased @entity224 production. To confirm the in vivo relevance of these findings, 20 healthy subjects consumed 7 mL. kg(-1). d(-1) of @entity70338 for 14 days. Platelet aggregation was inhibited after @entity70338 supplementation, platelet-derived NO production increased from 3.5+/-1.2 to 6.0+/-1.5 pmol/10(8) platelets, and @entity224 release decreased from 29.5+/-5.0 to 19.2+/-3.1 arbitrary units (P<0.007 and P<0.05, respectively). @entity1881 levels increased significantly after @entity70338 consumption (from 15.6+/-0.7 to 17.6+/-0.9 micromol/L; P<0.009), and the plasma protein-independent antioxidant activity increased by 50.0% (P<0.05). Last, incubation of platelets with select flavonoid fractions isolated from @entity70338 consistently attenuated @entity224 levels but had variable effects on whole-blood aggregation, @entity1423 , and NO release. CONCLUSIONS: Both in vitro incubation and oral supplementation with @entity70338 decrease @entity1423 , increase platelet-derived NO release, and decrease @entity224 production. These findings may be a result of antioxidant-sparing and/or direct effects of select @entity3088 found in @entity70338 . The suppression of platelet-mediated @entity715 represents a potential mechanism for the beneficial effects of purple grape products, independent of @entity167 consumption, in @entity65 .

Select XXXX and whole juice from purple grapes inhibit platelet function and enhance @entity842 release.

@entity63 :: ('MESH:D007511', 'Disease') :: ['coronary ischemia'] @entity1423 :: ('MESH:D001791', 'Disease') :: ['platelet aggregation'] @entity167 :: ('CHEBI:16236', 'Chemical') :: ['alcohol'] @entity70338 :: ('CHEBI:26346', 'Chemical') :: ['PGJ'] @entity3088 :: ('MESH:D005419', 'Chemical') :: ['flavonoids'] @entity1881 :: ('MESH:D024502', 'Chemical') :: ['alpha-Tocopherol'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity299 :: ('MESH:D006331', 'Disease') :: ['cardiac events'] @entity715 :: ('MESH:D013927', 'Disease') :: ['thrombosis'] @entity224 :: ('MESH:D013481', 'Chemical') :: ['superoxide'] @entity65 :: ('MESH:D002318', 'Disease') :: ['cardiovascular disease']
@entity3088 :: (MESH:D005419,Chemical) :: ['flavonoids']
