PURPOSE: We have previously demonstrated that systemic administration of @entity3033 (TP) can accelerate the functional recovery from hind limb @entity912 following @entity1930 in the @entity35 . In this study, we looked at the molecular mechanisms underlying this phenomenon. METHODS: Castrated adult male @entity35 received a right side sciatic nerve crush at the level of the sciatic notch, with the left side serving as control. Half the animals received a subcutaneous implant of TP, the others were sham implanted. Animals were sacrificed at 1, 3, 7 and 10 days post-operative. Lumbar spinal cord tissue was harvested and in situ hybridization was performed using a cytoskeletal cDNA probe complementary to betaII-tubulin. RESULTS: On the injured side, sciatic motoneuron tubulin mRNA levels were increased in alt groups at alt time points. At 3 and 7 days post- op, the TP treated group had significantly higher levels of tubulin mRNA. CONCLUSIONS: These results suggest that @entity251 enhances the rate of regeneration by increasing the neuronal cytoskeletal response after @entity21 . Further, these results, coupled with the results from previous experiments in other rodent models, suggest a common mechanism for gonadal @entity134 action on regenerating motoneurons across species.

Androgenic enhancement of betaII-tubulin mRNA in spinal motoneurons following XXXX .

@entity3033 :: ('MESH:D043343', 'Chemical') :: ['testosterone propionate'] @entity251 :: ('MESH:D013739', 'Chemical') :: ['testosterone'] @entity912 :: ('MESH:D010243', 'Disease') :: ['paralysis'] @entity134 :: ('CHEBI:35341', 'Chemical') :: ['steroid'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity21 :: ('MESH:D001480', 'Disease') :: ['axonal injury'] @entity1930 :: ('MESH:D020426', 'Disease') :: ['sciatic nerve injury']
@entity1930 :: (MESH:D020426,Disease) :: ['sciatic nerve injury']
