Massive increases in extracellular @entity935 have been reported in the @entity63 rodent striatum, implicating this neurotransmitter in toxic events. We have examined whether @entity935 receptor antagonists are protective against @entity519 insult, using brain slices containing the rostral striatum obtained from adult male C57/BLIcrfa( @entity19 . Slices were subjected in vitro to 20 min @entity1568 @entity519 , with or without addition of: (i) 50 microM @entity1767 ( @entity9767 antagonist and sigma ligand), (ii) 10 microM @entity10095 (selective @entity37746 antagonist), (iii) 10 microM @entity12174 (selective @entity9767 antagonist), (iv) 10 microM @entity10095 and 10 microM @entity12174 in combination, and (v) 10 microM @entity3390 (noncompetitive @entity143 receptor antagonist). Subsequently, slices were reoxygenated, fixed 2 h postinsult, and processed for light microscopy. Damage was assessed by calculating pyknotic profiles as a percentage of total neuronal profiles present. No pyknotic profiles were detected in normoxic control tissue, but this phenotype predominated in most slices subject to @entity519 alone (60.1 +/- 30.6% pyknotic profiles). Marked protection was produced by @entity1767 (7.1 +/- 7.6%, P = 0.002), @entity3390 (8.6 +/- 6.9%, P = 0.007), and the combined application of @entity10095 and @entity12174 (5.9 +/- 9.4%, P = 0.001). No protection was demonstrated for @entity10095 or @entity12174 when applied separately. These data suggest that @entity12807 in the rostral @entity19 striatum is mediated via @entity143 , D1, and D2 receptors. Protection against @entity12807 by @entity935 receptor antagonists requires the combined blockade of both classes of @entity935 receptor.

Protection of striatal neurons by joint blockade of D1 and D2 receptor subtypes in an in vitro model of XXXX .

@entity12807 :: ('MESH:D002534', 'Disease') :: ['hypoxic damage'] @entity10095 :: ('MESH:C534628', 'Chemical') :: ['SCH23390'] @entity1568 :: ('MESH:D009584', 'Chemical') :: ['nitrogen'] @entity37746 :: ('13488(Tax:10090)', 'Gene') :: ['D1 receptor'] @entity12174 :: ('MESH:C045989', 'Chemical') :: ['eticlopride'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemic'] @entity935 :: ('MESH:D004298', 'Chemical') :: ['dopamine'] @entity19 :: ('10090', 'Species') :: ['t) mice', 'mouse'] @entity519 :: ('MESH:D000860', 'Disease') :: ['hypoxic', 'hypoxia'] @entity143 :: ('CHEBI:6121', 'Chemical') :: ['NMDA'] @entity1767 :: ('MESH:D006220', 'Chemical') :: ['haloperidol'] @entity9767 :: ('13489(Tax:10090)', 'Gene') :: ['D2 receptor'] @entity3390 :: ('MESH:D016291', 'Chemical') :: ['MK-801']
@entity12807 :: (MESH:D002534,Disease) :: ['cerebral hypoxia']
