UNASSIGNED: @entity8786 mite is a predominant source of indoor allergens causing high incidence of @entity566 worldwide. @entity1 with different genetic background respond differently to the mite components, and thus the @entity2483 ( @entity2483 ) is preferred to the conventional @entity566 test based on crude mite extract. In this study, proteome and culprit components in the @entity8786 whole body extract that sensitized the @entity56 @entity1 were studied by using @entity214 -PAGE (1DE) and 2DE-IgE immunoblotting followed by LC- @entity15 / @entity15 and database search for protein identification. From the 1DE, the mite extract revealed 105 proteins that could be classified into seven functionally different groups: allergens, structural components, enzymes, enzyme inhibitor, receptor proteins, transporters, and binding/regulatory/cell signaling proteins. From the 2DE, the mite extract produced 94 spots; 63 were bound by IgE in sera of 20 @entity8786 @entity56 @entity1 . One more protein that was not revealed by the 2DE and protein staining reacted with IgE in 2 @entity56 @entity1 . Proteins in 40 spots could be identified as 35 different types. Three of them reacted to IgE of >50% of the @entity56 @entity1 , and hence they are major allergens: tropomyosin or Der f 10 (75%), @entity9276 hydratase (70%), and one uncharacterized protein (55%). Aconitate hydratase is a novel @entity8786 major allergen unraveled in this study. Several mite minor allergens that have never been previously reported are also identified. The data have clinical applications in the @entity2483 for tailor-designed allergen-specific immunotherapy.

Proteome, Allergenome, and Novel Allergens of House Dust Mite, XXXX .

@entity1 :: ('9606', 'Species') :: ['patients', 'People'] @entity566 :: ('MESH:D006967', 'Disease') :: ['allergy'] @entity9276 :: ('MESH:D000156', 'Chemical') :: ['aconitate'] @entity8786 :: ('6954', 'Species') :: ['Dermatophagoides farinae', 'D. farinae'] @entity56 :: ('MESH:D004342', 'Disease') :: ['allergic'] @entity2483 :: ('MESH:C566443', 'Disease') :: ['CRD', 'component-resolved diagnosis'] @entity15 :: ('MESH:D009103', 'Disease') :: ['MS'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS']
@entity8786 :: (6954,Species) :: ['Dermatophagoides farinae']
