PURPOSE: To explore the expression profile of the @entity1 lens and to provide a resource for microarray studies, expressed sequence tag (EST) analysis has been performed on cDNA libraries from adult lenses. METHODS: A cDNA library was constructed from two adult (40 year old) @entity1 lenses. Over two thousand clones were sequenced from the unamplified, un-normalized library. The library was then normalized and a further 2200 sequences were obtained. All the data were analyzed using GRIST (GRouping and Identification of Sequence Tags), a procedure for gene identification and clustering. RESULTS: The lens library (by) contains a low percentage of non-mRNA contaminants and a high fraction (over 75%) of apparently full length cDNA clones. Approximately 2000 reads from the unamplified library yields 810 clusters, potentially representing individual genes expressed in the lens. After normalization, the content of crystallins and other abundant cDNAs is markedly reduced and a similar number of reads from this library (fs) yields 1455 unique groups of which only two thirds correspond to named genes in GenBank. Among the most abundant cDNAs is one for a novel gene related to @entity1160 synthetase, which was designated "lengsin" (LGS). Analyses of ESTs also reveal examples of alternative transcripts, including a major alternative splice form for the lens specific membrane protein @entity12343 . Variant forms for other transcripts, including those encoding the apoptosis inhibitor @entity22769 and the armadillo repeat protein @entity68970 , are also described. CONCLUSIONS: The lens cDNA libraries are a resource for gene discovery, full length cDNAs for functional studies and microarrays. The discovery of an abundant, novel transcript, lengsin, and a major novel splice form of @entity12343 reflect the utility of unamplified libraries constructed from dissected tissue. Many novel transcripts and splice forms are represented, some of which may be candidates for @entity74 .

Expressed sequence tag analysis of adult XXXX lens for the NEIBank Project: over 2000 non-redundant transcripts, novel genes and splice variants.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1160 :: ('CHEBI:28300', 'Chemical') :: ['glutamine'] @entity68970 :: ('421', 'Gene') :: ['ARVCF'] @entity12343 :: ('3982', 'Gene') :: ['MP19'] @entity74 :: ('MESH:D030342', 'Disease') :: ['genetic diseases'] @entity22769 :: ('79444', 'Gene') :: ['Livin']
@entity1 :: (9606,Species) :: ['human']
