BACKGROUND: Since the identification of @entity8265 ( @entity8265 ) as the antigen for the @entity1701 ( @entity1701 ), several antigen-specific immunoassays have been reported for @entity1593 ( @entity1593 ) screening. A first objective was to evaluate the suitability for @entity1593 screening of three different IgA @entity8265 ELISAs, two of them based on @entity840 liver @entity8265 (gp- @entity8265 ) (an in-house ELISA with a partially purified extract and a commercial ELISA with purified gp- @entity8265 antigen) and a third recombinant @entity1 @entity8265 (rh- @entity8265 ) ELISA. The results are compared with @entity1701 and with the final clinical diagnosis. A second objective was to analyze antibody reactivities in those patients with anti- @entity8265 and @entity1701 . METHODS: ELISA and @entity1701 tests were used to measure IgA anti- @entity8265 levels in sera from 259 @entity1 (107 had @entity1593 and 72 had @entity6 ). RESULTS: The purified gp- @entity8265 ELISA was highly sensitive (97.7%) and specific (98.8%) in the detection of @entity1593 , almost equaling @entity1701 . Rh- @entity8265 ELISA did not improved the sensitivity of @entity1701 , but its specificity was slightly superior. Immunoblot analysis with partially purified gp- @entity8265 extract, the antigen most frequently used for anti- @entity8265 detection, showed that the majority of false positives were due to IgA reactivities to contaminant proteins present in the liver antigenic extract. This low specificity was particularly problematic in diabetics. CONCLUSION: Purified @entity8265 ELISAs, either with purified @entity840 liver or recombinant human antigens, can be used as quantitative and observer-independent alternatives to the traditional and time-consuming @entity1701 in the screening of @entity1593 .

Anti-transglutaminase IgA ELISA: clinical potential and drawbacks in XXXX diagnosis.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity1593 :: ('MESH:D002446', 'Disease') :: ['celiac disease', 'CD'] @entity6 :: ('MESH:D003920', 'Disease') :: ['Type I diabetes mellitus'] @entity840 :: ('10141', 'Species') :: ['guinea pig'] @entity1701 :: ('MESH:D016736', 'Disease') :: ['EMA discrepancies', 'anti-endomysial antibodies', 'EMA'] @entity8265 :: ('7052', 'Gene') :: ['tissue transglutaminase', 'tTG']
@entity1593 :: (MESH:D002446,Disease) :: ['celiac disease']
