@entity10273 ( @entity10273 ) is a 56 kDa collagenous protein that promotes attachment of mesenchymal cells. Previous studies have shown that the presence of @entity10273 is restricted to cementum in adult @entity1 tissues. In this study, we report generation of a monoclonal antibody against @entity10273 and its use for the investigation of @entity10273 in developing @entity291 tooth germs. @entity19 were immunized with @entity10273 purified from @entity291 cementum, and a monoclonal antibody, 3G9, was produced. Immunohistochemical staining of @entity291 tooth germ at root forming stage using 3G9 antibody showed that the tissue distribution of @entity10273 expression was limited to cementum matrix and cementoblasts during cementogenesis. Alveolar bone did not stain with the 3G9 antibody, whereas anti-type I collagen stained positively. @entity10273 was purified from @entity291 tooth germs with immunoaffinity purification using the 3G9 antibody. Examination of the immunoaffinity-purified fraction showed that @entity10273 existed in tooth germ as a 65 kDa protein. The protein was susceptible to bacterial collagenase. To investigate the possible biological function of @entity10273 during cementogenesis, we isolated dental follicle cells from the @entity291 tooth germ, and showed that they adhered to surfaces containing @entity10273 . These data demonstrate that @entity10273 is expressed by @entity291 cementoblasts as a 65 kDa protein and that the @entity10273 may have a function in cementogenesis.

Expression of cementum-derived attachment protein in XXXX tooth germ during cementogenesis.

@entity1 :: ('9606', 'Species') :: ['human'] @entity19 :: ('10090', 'Species') :: ['Mice'] @entity291 :: ('9913', 'Species') :: ['bovine'] @entity10273 :: ('MESH:D019962', 'Disease') :: ['CAP', 'Cementum-derived attachment protein']
@entity291 :: (9913,Species) :: ['bovine']
