@entity11920 ( @entity11920 ) and 2B (CYP2B) mRNA and protein expression was examined in primary cultured @entity35 hepatocytes under basal cell culture conditions and in response to three prototypic @entity11920 inducers, i.e. @entity1068 , @entity1324 , and @entity34878 . Xenobiotic treatment for 24 hr, initiated after hepatocytes had been maintained in culture for 72 hr, resulted in 2-8-fold increases in @entity11920 protein levels, relative to untreated cells. A >/=2-fold increase in @entity11920 protein levels was detected at the lowest concentration (1 mM) of each of the xenobiotics examined. The increase in @entity11920 protein expression was not accompanied by any significant increase in 2E1 mRNA expression. In contrast, CYP2B protein and mRNA levels were increased by @entity1324 or @entity34878 at concentrations greater than 1 mM. @entity1068 (up to 100 mM) failed to significantly increase CYP2B protein or mRNA levels. The maximal increases measured for CYP2B protein and mRNA ( approximately 25-fold and approximately 90-fold, respectively) after treatment of hepatocytes with @entity1324 were comparable to those measured in our laboratory, and reported by others, for @entity109 treatment of primary cultured @entity35 hepatocytes. The results of this study show that the pattern of expression of @entity11920 B in this primary cultured @entity35 hepatocyte system and the magnitude of induction parallel those reported for @entity35 liver in vivo in response to these xenobiotics. This primary hepatocyte culture system provides opportunities for studies of the role of @entity11920 in the metabolism and bioactivation of drugs, chemicals, and putative carcinogens, as well as mechanistic studies on xenobiotic-mediated regulation of @entity11920 expression.

Xenobiotic-enhanced expression of cytochromes P450 2E1 and 2B in primary cultured XXXX hepatocytes.

@entity1324 :: ('MESH:D000096', 'Chemical') :: ['acetone'] @entity11920 :: ('25086(Tax:10116)', 'Gene') :: ['CYP2E1', 'CYP2E1 and 2', 'Cytochrome P450 2E1'] @entity109 :: ('MESH:D010634', 'Chemical') :: ['phenobarbital'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity34878 :: ('CHEBI:30953', 'Chemical') :: ['pyrazine'] @entity1068 :: ('MESH:D000431', 'Chemical') :: ['Ethanol', 'ethanol']
@entity35 :: (10116,Species) :: ['rat']
