UNASSIGNED: The aim of the present study was to examine the impact of low-frequency, low-intensity ultrasound (US)-stimulated microbubbles (USMB) on radiofrequency ablation (RFA) in the treatment of @entity19 with @entity1 @entity263 xenografts. The @entity5  -bearing @entity19 were divided into three groups: The USMB+RFA group was treated with USMB immediately followed by RFA, the RFA group was treated with RFA alone, and the control group remained untreated. The animals underwent enhanced US to calculate the @entity5 volumes, ablation volumes and ablation rates. Subsequently, the @entity5 were excised for @entity1710 and @entity1711 staining, to identify @entity39 in the @entity5 following the treatments, and immunohistochemical staining, to analyze the apoptotic index (AI), proliferative index (PI) and microvessel density (MVD) at 1, 4 and 7  days post-treatment. Each group contained five @entity19 at each time  -point. Necrosis was apparent in the center of the @entity5 in the treatment groups. Ablation lesion volumes of the USMB+RFA group were larger than those in the RFA group at 1 and 4  days post  -treatment (P=0.002 and P=0.022, respectively), and the ablation rates of the USMB+RFA group were significantly higher, compared with the RFA group at the three time  -points (all P<0.001). There were fewer apoptotic cells and more proliferative cells in the RFA group, compared with the control group 1,4 and 7  days post  -treatment (all P<0.05). The AI of the USMB+RFA group was higher than that of the control group and lower than that of the RFA group 1  day post-treatment (P=0.034 and P=0.016, respectively). The PI of the USMB+RFA group was lower than that of the control group and higher than that of the RFA group 4  and 7  days post-treatment (all P<0.05). No significant differences were observed in MVD among the three groups throughout the experiment. In conclusion, exposure to USMB prior to RFA produced larger volumes of ablation, compared with treatment with RFA alone, and increased AI and reduced PI in the residual @entity189 cells induced by RFA.

Low-frequency and low-intensity ultrasound-mediated microvessel disruption enhance the effects of radiofrequency ablation on @entity263 xenografts in XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity263 :: ('MESH:D011471', 'Disease') :: ['prostate cancer'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis'] @entity19 :: ('10090', 'Species') :: ['mice', 'nude mice'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinoma'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hematoxylin']
@entity19 :: (10090,Species) :: ['nude mice']
