PURPOSE: Tissue autofluorescence study is a promising means of endoscopic detection of @entity694 , but the mechanism of autofluorescence eruption has still not been verified. The purpose of this study was to precisely analyze the autofluorescence characteristics of freshly prepared normal @entity35 colon under UVA and violet light excitation. METHODS: Excised @entity35 colons were studied by using multichannel spectrophotometry, spectroscopic imaging, confocal microscopy, combined two-photon excited fluorescence and second-harmonic generation (SHG) microscopy, and fluorescence lifetime imaging microscopy. RESULTS: Spectroscopic analysis of freshly prepared colon sections revealed that the mucosa and the submucosa showed strong autofluorescence under UVA and violet light excitation. The combined images of two-photon and SHG microscopy revealed that the @entity33 are the important source of autofluorescence. @entity2125 seems to be one of the major substances involved in the autofluorescence of the mucosal layer on 365- nm light excitation. The autofluorescence spectra of the luminal surfaces were identical to those of the mucosa on crosssectional examinations with 365-nm excitation. The main origin of autofluorescence of the luminal surface with 365-nm excitation is the epithelial cells in the mucosa without overlay of submucosal fluorescence. CONCLUSIONS: The mucosal layer is the important source of the autofluorescence observed under excitation with UVA/violet light in multilayered @entity694 . Illumination of 365-nm wavelength light is a suitable means of analyzing the autofluorescence of @entity33 .

Precise analysis of the autofluorescence characteristics of XXXX colon under UVA and violet light excitation.

@entity35 :: ('10116', 'Species') :: ['rat'] @entity2125 :: ('MESH:D009243', 'Chemical') :: ['Nicotinamide adenine dinucleotide'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colonic structures', 'colonic neoplasia'] @entity33 :: ('MESH:D052016', 'Disease') :: ['mucosal epithelia']
@entity35 :: (10116,Species) :: ['rat']
