OBJECTIVE: The purpose of the present study was to compare the permeability and regulation of paracellular transport in @entity1 cervical cells with those in epithelial cells of other organs. METHODS: Cervical cells (ECE16-1, Caski, and HT3) were grown on filters, and transepithelial electrical conductance ( @entity1422 ) and the permeability to @entity18855 (PPyr) were determined. RESULTS: Cervical cultures were characterized by high @entity1422 (83-125 mS.cm-2) and high PPyr (6.2-18 x 10(-6). @entity8639 ). The @entity1422 was not significantly affected by cell density but was increased by 20% by lowering extracellular @entity460 to 0.45 mmol/L or less. The high values of @entity1422 and PPyr and the regulation by extracellular @entity460 indicate that all three cervical cell lines have "leaky" tight junctional complexes. Addition of extracellular @entity855 ( @entity855 ) at 50 mumol/L to the cervical cultures evoked a biphasic change in @entity1422 that was unique to the cervical cells: an initial increase, followed by a sustained decrease by 30% from baseline @entity1422 . The decrease of @entity1422 was associated with a decrease in PPyr by 17%, indicating that @entity855 had an effect on the tight junctional/paracellular permeability. The @entity855 effect was reversible either by washing or by chemical hydrolysis with ATPase. The non-cervical cell lines all responded to extracellular @entity855 with a transient increase in @entity1422 , but not with the pronounced decrease. CONCLUSION: The permeability of the paracellular pathway can be regulated in cervical epithelia by mechanisms that may be different from those in epithelial cells from other organs.

Characterization of paracellular permeability in cultured @entity1 cervical epithelium: regulation by extracellular XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity8639 :: ('653677', 'Gene') :: ['sec-1'] @entity18855 :: ('MESH:C005047', 'Chemical') :: ['pyranine'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity1422 :: ('MESH:D013915', 'Disease') :: ['GT'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['adenosine triphosphate', 'ATP']
@entity855 :: (MESH:D000255,Chemical) :: ['adenosine triphosphate']
