Bacterial beta-galactosidase cDNA was injected without lipofectin into 41 sites in @entity235 muscle and expression was seen in 22 of them. The cDNA and lipofectin was injected into 35 similar sites and expression was seen in 21. Expression was seen in a maximum of 2.5% of muscle fibers and 23.21% of nonmuscle cells. A total of 106 muscle sites were injected with the minigene with and without lipofectin. In 4 of the 45 sites injected with the minigene without lipofectin @entity1 @entity4714 was expressed around the periphery of 0.3% of the fibers. Bacterial beta-galactosidase cDNA was injected into the peritoneal cavity of 4 pups, 2 of which also received lipofectin. In all 4, expression was seen in liver, spleen, and mesenteric lymph node. In the 2 pups that received lipofectin, expression was also seen in the diaphragm, intercostal, and abdominal muscles of 1 and in the diagphragm and intercostal muscles of the other. These experiments show that @entity1 @entity4714 transgene expression can be obtained in @entity235 muscle. However, other methods will be required to increase the degree of expression before gene therapy trials can be undertaken.

Direct dystrophin and reporter gene transfer into XXXX muscle in vivo.

@entity235 :: ('9615', 'Species') :: ['dog'] @entity1 :: ('9606', 'Species') :: ['human'] @entity4714 :: ('1756', 'Gene') :: ['dystrophin']
@entity235 :: (9615,Species) :: ['dog']
