GINS is a key component of the eukaryotic @entity22 ( @entity22 )-GINS (CMG) complex, which unwinds duplex DNA at the moving replication fork. Archaeal GINS complexes have been shown to stimulate the @entity69837 activity of their cognate @entity22 mainly by elevating its ATPase activity. Here, we report that GINS from the thermoacidophilic crenarchaeon @entity12204 (SsoGINS) is capable of DNA binding and binds preferentially to single-stranded DNA (ssDNA) over double-stranded DNA (dsDNA). Notably, SsoGINS binds more strongly to dsDNA with a 5' ssDNA tail than to dsDNA with a 3' tail and more strongly to an ssDNA fragment blocked at the 3' end than to one at the 5' end with a @entity1885 -streptavidin (SA) complex, suggesting the ability of the protein complex to slide in a 5'-to-3' direction along ssDNA. DNA-bound SsoGINS enhances DNA binding by SsoMCM. Furthermore, SsoGINS increases the @entity69837 activity of SsoMCM. However, the ATPase activity of SsoMCM is not affected by SsoGINS. Our results suggest that SsoGINS facilitates processive DNA unwinding by SsoMCM by enhancing the binding of the @entity69837 to DNA. We propose that SsoGINS stabilizes the interaction of SsoMCM with the replication fork and moves along with the @entity69837 as the fork progresses. IMPORTANCE: GINS is a key component of the eukaryotic Cdc45- @entity22 -GINS complex, a molecular motor that drives the unwinding of DNA in front of the replication fork. Archaea also encode GINS, which interacts with @entity22 , the @entity69837 . But how archaeal GINS serves its role remains to be understood. In this study, we show that GINS from the hyperthermophilic archaeon @entity12204 is able to bind to DNA and slide along ssDNA in a 5'-to-3' direction. Furthermore, @entity12204 GINS enhances DNA binding by @entity22 , which slides along ssDNA in a 3'-to-5' direction. Taken together, these results suggest that @entity12204 GINS may stabilize the interaction of @entity22 with the moving replication fork, facilitating processive DNA unwinding.

The XXXX GINS Complex Stimulates DNA Binding and Processive DNA Unwinding by Minichromosome Maintenance @entity69837 .

@entity1885 :: ('MESH:D001710', 'Chemical') :: ['biotin'] @entity69837 :: ('24868091', 'Gene') :: ['helicase'] @entity12204 :: ('2287', 'Species') :: ['Sulfolobus solfataricus', 'Sulfolobus'] @entity22 :: ('MESH:D007319', 'Disease') :: ['MCM', 'Cdc45-minichromosome maintenance']
@entity12204 :: (2287,Species) :: ['Sulfolobus solfataricus']
