OBJECTIVE: To assess in vitro and in vivo the potential utility of electropermeabilization (EP) as an anticancer drug delivery system for the treatment of @entity1606 ( @entity1606 ). MATERIALS AND METHODS: To analyse the effects of EP on the internalization of @entity632 and @entity1732 by cells, aliquots of a suspension of YTS-1 @entity189 cells (derived from a @entity1 @entity1606 line) were mixed with a solution of @entity632 or @entity1732 and then exposed immediately to an electric field (1000 V/cm, 1 Hz, 100-micros square wave, eight pulses). After a 2-h incubation the concentration of each drug in the cells was measured by high-performance liquid chromatography (for @entity632 ) and by bioassay (for @entity1732 ). The concentrations of drugs in the cells were compared with untreated cells. To analyse the effects of EP on @entity137 , the same treatments were applied to a suspension of cells plus @entity632 or @entity1732 and then the cells incubated for 6 h with tritiated @entity1014 ( @entity665 ), monitoring the incorporation of @entity665 into the cells. Cells with no electrotreatment acted as controls. To assess the effects on @entity5 in vivo, YTS-1 cells were transplanted subcutaneously into @entity19 ; when the @entity5 had reached 7 mm in diameter, EP was applied (using electrical pulses as before, 10 min after the direct injection of @entity1732 into the @entity5 ). @entity5 were then measured regularly and compared with sham-treated @entity5 , @entity5 treated with electric pulses alone, and @entity5 treated with @entity1732 alone. Survival was also compared. RESULTS: There were no significant differences in the levels of @entity632 between cells with and with no EP, whereas there was a marked difference for @entity1732 . Growth inhibition by @entity632 with and with no EP was similar, while the growth-inhibitory effects of @entity1732 were almost doubled. There was a reduction in @entity5 size only in the group treated with @entity1732 plus electric pulses and two of five @entity5 disappeared completely. Survival in this group was also significantly better than in the other groups. CONCLUSION: EP after administering @entity1732 might be an effective treatment for @entity1606 .

Effects of electropermeabilization after the administration of anticancer drugs on XXXX .

@entity632 :: ('MESH:D004317', 'Chemical') :: ['adriamycin'] @entity1 :: ('9606', 'Species') :: ['human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumour', 'Tumours', 'tumours'] @entity19 :: ('10090', 'Species') :: ['nude mice'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinoma'] @entity665 :: ('CHEBI:53526', 'Chemical') :: ['[3H]-TdR'] @entity1606 :: ('MESH:D002295', 'Disease') :: ['transitional cell carcinoma', 'TCC'] @entity1732 :: ('MESH:D001761', 'Chemical') :: ['bleomycin'] @entity1014 :: ('MESH:D013936', 'Chemical') :: ['thymidine']
@entity1606 :: (MESH:D002295,Disease) :: ['transitional cell carcinoma']
