Alcaligenes latus has been known to produce @entity13099 ( @entity13100 ) in a growth-associated manner even under nutrient-sufficient conditions. However, the @entity13100 content obtained by fed-batch culture was always low, at ca. 50%, which makes the recovery process inefficient. In this study, the effect of applying @entity1568 limitation on the production of @entity13100 by A. latus was examined. In flask and batch cultures, the @entity13100 synthesis rate could be increased considerably by applying @entity1568 limitation. The @entity13100 content could be increased to 87% by applying @entity1568 limitation in batch culture, which was considerably higher than that typically obtainable (50%) under @entity1568 -sufficient conditions. In fed-batch culture, cells were first cultured by the DO-stat feeding strategy without applying @entity1568 limitation. @entity1568 limitation was applied at a cell concentration of 76 g (dry cell weight)/liter, and the @entity969 concentration was maintained within 5 to 20 g/liter. After 8 h of @entity1568 limitation, the cell concentration, @entity13100 concentration, and @entity13100 content reached 111.7 g (dry cell weight)/liter, 98.7 g/liter, and 88%, respectively, resulting in a productivity of 4.94 g of @entity13100 /liter/h. The highest @entity13100 productivity, 5.13 g/liter/h, was obtained after 16 h.

@entity13099 Production with High Productivity and High Polymer Content by a Fed-Batch Culture of Alcaligenes latus under XXXX Limitation.

@entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity1568 :: ('MESH:D009584', 'Chemical') :: ['Nitrogen', 'nitrogen'] @entity13100 :: ('CHEBI:53388', 'Chemical') :: ['PHB'] @entity13099 :: ('MESH:C003182', 'Chemical') :: ['poly(3-hydroxybutyrate)']
@entity1568 :: (MESH:D009584,Chemical) :: ['Nitrogen']
