BACKGROUND _ AIMS: High doses of radiation induce severe DNA damage in intestinal epithelial cells, especially crypt cells, and cause @entity1944 , but the underlying molecular mechanisms remain unclear. Kr  ppel-like factor 5 ( @entity70209 ), a @entity219 finger-containing transcription factor, is induced by various stress stimuli and is involved in cell proliferation and survival. The role of @entity70209 in radiation-induced @entity1944 was investigated here. METHODS: Wild type @entity19 were treated with 8 or 15 Gy @entity348 ( @entity348 ). @entity70209 content and cellular localization in the small intestines of irradiated @entity19 were detected by Western blot and immunohistochemical analysis. @entity19 with intestinal-specific knockdown of @entity70209 ( @entity34745 -Cre; @entity70209 (fl/+) @entity19 ) were generated and their response to radiation was compared with controls. Morphological changes were determined by @entity1710 and @entity1711 staining. Proliferation was examined by @entity14160 immunostaining. The molecular response of the small intestine after @entity70209 knockdown was investigated using microarrays. RESULTS: @entity70209 expression correlated with the progression of @entity16 . Decreased levels of @entity70209 in the gut were associated with increased damage to the intestinal mucosa and reduced epithelial proliferation after @entity348 . Our microarray data disclosed that @entity70209 knockdown down-regulated genes related to DNA damage repair pathways such as @entity4334 excision repair, mismatch repair, non-homologous end joining and the @entity11807 pathway, which may suggest a novel function of @entity70209 . CONCLUSIONS: Our study illustrates that @entity70209 may modulate DNA repair pathways to prevent @entity1944 induced by @entity348 . @entity70209 signaling provides a novel field for identification of potential therapeutic targets for the treatment of radiation-induced @entity16 .

Kr  ppel-Like Factor 5 Promotes Epithelial Proliferation and DNA Damage Repair in the Intestine of Irradiated XXXX .

@entity1944 :: ('MESH:D007410', 'Disease') :: ['intestinal injury'] @entity70209 :: ('12224(Tax:10090)', 'Gene') :: ['Klf5', 'KLF5'] @entity14160 :: ('17345(Tax:10090)', 'Gene') :: ['Ki67'] @entity19 :: ('10090', 'Species') :: ['mice', 'Mice'] @entity1711 :: ('MESH:D004801', 'Chemical') :: ['eosin'] @entity219 :: ('MESH:D015032', 'Chemical') :: ['zinc'] @entity34745 :: ('22349(Tax:10090)', 'Gene') :: ['Vil'] @entity16 :: ('MESH:D009422', 'Disease') :: ['intestinal damage'] @entity1710 :: ('MESH:D006416', 'Chemical') :: ['hematoxylin'] @entity348 :: ('MESH:C535338', 'Disease') :: ['TBI', 'total body irradiation'] @entity4334 :: ('D009711', 'Chemical') :: ['nucleotide'] @entity11807 :: ('MESH:D005199', 'Disease') :: ['Fanconi anemia']
@entity19 :: (10090,Species) :: ['Mice']
