Tumor biological factors @entity11261 , @entity2289 , @entity10815 , S-phase fraction (SPF), @entity1758 (Ki-67), @entity876 , and @entity1722 were assessed in 100 node-negative @entity0 @entity1 . Their prognostic impact on disease-free (DFS) as well as overall survival (OS) was compared to that of traditional factors @entity5 size, grading, and @entity134 hormone receptor status. Antigen levels of @entity11261 , its inhibitor @entity2289 , and @entity10815 were determined in @entity5 tissue extracts by immunoenzymatic methods. SPF was determined by flow cytofluorometry, @entity1758 , @entity876 , and @entity1722 /neu by immunohistochemistry in adjacent routinely @entity406 -fixed paraffin sections. Median follow-up in all @entity1 still alive at time of analysis was 76 months. Univariate analysis determined @entity2289 (p = 0.0001), @entity11261 (p = 0.0437), @entity1758 (p = 0.0214), and SPF (p = 0.0248) as statistically significant prognostic factors for DFS. In contrast, @entity5 size, @entity134 hormone receptor status, grading, @entity876 , @entity1722 , and @entity10815 failed to be of prognostic value. In multivariate analysis, including the statistically significant prognostic factors @entity2289 , @entity11261 , @entity1758 , and SPF, only @entity2289 (p = 0.0003, relative risk: 4.7) proved to be of independent statistical significance for DFS. Regarding OS, @entity2289 was the only statistically significant prognostic factor in univariate (p = 0.0001) as well as multivariate analysis (p = 0.0000, relative risk: 7.1). Thus, factors describing the invasive and metastatic capacity of @entity5 cells ( @entity11261 , @entity2289 ) and factors related to their proliferative activity (SPF, @entity1758 ) provide valuable prognostic information in node-negative @entity0 @entity1 .

Prognostic impact of XXXX biological factors on survival in node-negative @entity0 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity10815 :: ('1509', 'Gene') :: ['cathepsin D', 'cathepsin. D'] @entity2289 :: ('5054', 'Gene') :: ['PAI-1'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity1722 :: ('2064', 'Gene') :: ['HER-2', 'HER-2/neu'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity406 :: ('MESH:D005557', 'Chemical') :: ['formalin'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity134 :: ('CHEBI:35341', 'Chemical') :: ['steroid'] @entity1758 :: ('57534', 'Gene') :: ['MIB1'] @entity11261 :: ('118471', 'Gene') :: ['uPA']
@entity5 :: (MESH:D009369,Disease) :: ['tumor']
