INTRODUCTION: @entity1067 causes partial @entity1876 (pBOO), and many @entity1 with pBOO are affected by not only voiding symptoms but also storage symptoms. We previously suggested that enhancement of @entity750 ( @entity750 )-induced @entity1494 in the pBOO bladder may be one cause of storage symptoms. However, little is known about the presence of @entity750 in @entity35 bladders. In this study, we hypothesized that mast cells are a source of @entity750 and investigated the distribution of mast cells and @entity750 in the bladders of @entity35 with pBOO. METHODS: The bladders of female @entity35 were subjected to pBOO and sham operations for 1  week, were isolated, and were fixed for light or electron microscopy. Mast cells and @entity750 in the bladders were detected by @entity11979 staining and immunohistochemical staining, respectively. The mast cells were counted under a light microscope. Degranulated mast cells were observed under an electron microscope and counted under a light microscope. RESULTS: Mast cells were present in the mucosa/submucosa region in sham @entity35 bladders. Their number was increased in the detrusor muscle/subserosa/serosa region, especially the subserosal layer, in pBOO @entity35 bladders. The localization of mast cells almost matched that of @entity750 -positive cells in consecutive sections. Degranulated mast cells were present in sham and pBOO @entity35 bladders, but the proportion of degranulated mast cells was significantly increased in every region in pBOO @entity35 bladders compared with that in sham @entity35 bladders. CONCLUSION: These results suggest that mast cells contain @entity750 and are more abundant locally in the subserosal layer of pBOO @entity35 bladders. @entity750 released from mast cells could stimulate 5-HT2 receptors on the detrusor muscle, and this may underlie storage symptoms. FUNDING: Asahi Kasei Pharma Corp.

Mast Cell Accumulation and Degranulation in XXXX Bladder with Partial Outlet Obstruction.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity1494 :: ('MESH:D001745', 'Disease') :: ['bladder contraction'] @entity1067 :: ('MESH:D011470', 'Disease') :: ['Benign prostatic hyperplasia'] @entity11979 :: ('MESH:D014048', 'Chemical') :: ['toluidine blue'] @entity750 :: ('MESH:D012701', 'Chemical') :: ['5-hydroxytryptamine', '5-HT'] @entity35 :: ('10116', 'Species') :: ['rat', 'Sprague-Dawley rats', 'rats'] @entity1876 :: ('MESH:D001748', 'Disease') :: ['bladder outlet obstruction']
@entity35 :: (10116,Species) :: ['Rat']
