Wear particles from prosthetic implants have been shown to cause @entity32 and @entity3564 . These particle-induced pathologies are manifestations of adverse cellular responses to phagocytosed particles. In this study, phagocytosis of @entity691 particles was analyzed using flow cytometry (FCM), and the clinical utility of FCM in diagnosing particle-induced @entity603 was examined. Ultra high molecular weight @entity691 particles exhibited natural autofluorescence at @entity2822 wavelengths when determined by FCM. Using this autofluorescent property of the particles, peripheral blood monocytes (PBMs) phagocytosing the particles could be detected by autofluorescence emission from intracellular particles. This autofluorescence from PBMs increased with particles/cell ratio in a dose-dependent manner. Particle phagocytosis was also detectable in joint fluid cells obtained from the @entity1 with particle-induced @entity603 following @entity2720 ( @entity2720 ). Phenotypic analysis indicated that phagocytes were typically @entity5750 (+) @entity4071 (-) macrophages, with occasional @entity5750 (+) @entity4071 (+) macrophages. Interestingly, decreased autofluorescence intensity of @entity5750 (+) cells was observed after arthroscopic drainage, suggesting that FCM was useful in examining whether the treatment was successful. In summary, these results indicate that FCM analysis offers a simple and useful method of detecting phagocytosis of @entity691 particles and estimating the severity of particle-induced @entity603 in post- @entity2720 @entity1 .

Flow cytometric technique for the detection of phagocytosed wear particles in XXXX with @entity2720 .

@entity691 :: ('MESH:D020959', 'Chemical') :: ['polyethylene'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammatory synovitis'] @entity1 :: ('9606', 'Species') :: ['patients'] @entity5750 :: ('929', 'Gene') :: ['CD14'] @entity2822 :: ('CHEBI:37926', 'Chemical') :: ['fluorescein isothiocyanate'] @entity603 :: ('MESH:D013585', 'Disease') :: ['synovitis'] @entity4071 :: ('2214', 'Gene') :: ['CD16'] @entity2720 :: ('MESH:D007592', 'Disease') :: ['total joint arthroplasty', 'TJA'] @entity3564 :: ('MESH:D057068', 'Disease') :: ['periprosthetic osteolysis']
@entity1 :: (9606,Species) :: ['patients']
