BACKGROUND: The antitumor effects of antiplatelet agents in @entity356 cells are not well known. In this study, the possibility of @entity356 treatment with an antiplatelet agent, mainly @entity117 , was examined both in vivo and in vitro. METHODS: For in vivo experiments, @entity5 -bearing @entity19 were treated by an antiplatelet antibody or @entity117 , and the @entity5 growth was compared. For in vitro experiments, @entity1 @entity356 cell lines were used to confirm the @entity5 cell growth and inhibition by reducing the platelet count or using @entity117 . We also examined several cytokines by using an ELISA assay and conducted microRNA microarray analysis of @entity1 @entity5 cells to determine the influence of platelets or @entity117 . RESULTS: In vivo experiments showed that @entity5 growth was inhibited by halving the circulating platelet count by using an antiplatelet antibody or peroral daily @entity117 . In vitro experiments showed that the proliferation rates of @entity356 cell lines were increased after coincubation with platelets and that the effect was inhibited by @entity117 . Although the expression of @entity650 , platelet-derived growth factor, transforming growth factor-b, and @entity1421 did not correlate with @entity5 growth inhibition by @entity117 , seven microRNAs showed altered expression in @entity5 cells in response to coincubation with platelets or addition of @entity117 . Cells transfected with mir-4670-5p showed a significant increase in proliferation compared to negative control cells. CONCLUSIONS: Our study showed that platelets increased the proliferation of @entity356 cells and that this increase was inhibited by antiplatelet antibody or @entity117 . Mir-4670-5p may play an important role in these responses.

Antitumor effect of antiplatelet agents in XXXX cells: an in vivo and in vitro study.

@entity1 :: ('9606', 'Species') :: ['MKN-45', 'human'] @entity650 :: ('3569', 'Gene') :: ['interleukin-6'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity117 :: ('MESH:D001241', 'Chemical') :: ['aspirin'] @entity1421 :: ('MESH:D015232', 'Chemical') :: ['prostaglandin E2'] @entity356 :: ('MESH:D013274', 'Disease') :: ['gastric cancer']
@entity356 :: (MESH:D013274,Disease) :: ['gastric cancer']
