UNASSIGNED: Kisspeptin (Kp) and @entity68739 ( @entity68739 ) expressions have been reported to be in the placenta, and a possible involvement of the @entity68739 /Kps system in regulating trophoblast invasion and proliferation has been hypothesized. The aim of the present study was to investigate whether @entity68739 activation by kisspeptin-10 (Kp-10) could modulate in  vitro proliferation and @entity1420 (P4) secretion of @entity291 primary placental cell lines isolated from cotyledons of fetuses in the first trimester of pregnancy. The involvement of @entity68739 in the cell responses observed was also analyzed. Uteri from @entity291 at the first trimester of pregnancy were obtained from local abattoirs. Fetal cotyledon fragments were digested with collagenase in low @entity413 Dulbecco's Modified Eagle's Medium and cell lines were isolated. After being characterized for epithelial polygonal morphology, the presence of binucleate cells, male gender, and the expression of cytokeratin and zona occludens 2, cell lines were cultured in a low @entity413 Dulbecco's Modified Eagle's Medium-based expansion medium in the presence of 0.01, 0.1, 1, and 10   M Kp-10. Control cells were cultured in the absence of Kp-10. Cell population doubling time was evaluated for each culture passage (P) from P1 to P10. Cells were tested for @entity68739 mRNA expression analysis by real-time reverse transcription-polymerase chain reaction, and culture media were analyzed for P4 concentration by radioimmunoassay. Kisspeptin-10 modulated in  vitro proliferation of epithelial cell lines isolated from cotyledons recovered from @entity291 fetuses in the first trimester of pregnancy. Inhibitory (line A) or stimulatory (line B) effects of Kp-10 on cell proliferation were found in different cell lines and observed cell responses were found to be related to @entity68739 mRNA levels. Inhibition of cell proliferation matched with not significant variation of @entity68739 expression, whereas stimulation of cell proliferation was found to be related to @entity68739 upregulation. In both cell lines, no effect of Kp-10 on P4 secretion was found at any tested concentration. These results lead to the conclusion that the @entity68739 /Kps system is involved in the regulation of cell proliferation of @entity291 placental cotyledon cell lines isolated at the first trimester of pregnancy but, at this gestational stage, it may not be involved in modulating placental P4 secretion.

Effects of kisspeptin-10 on in  vitro proliferation and kisspeptin receptor expression in primary epithelial cell cultures isolated from XXXX placental cotyledons of fetuses at the first trimester of pregnancy.

@entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity291 :: ('9913', 'Species') :: ['cows', 'bovine'] @entity68739 :: ('528699(Tax:9913)', 'Gene') :: ['Kiss-1R', 'Kiss-1 receptor'] @entity1420 :: ('MESH:D011374', 'Chemical') :: ['progesterone']
@entity291 :: (9913,Species) :: ['bovine']
