The findings of this study represent the first report, to the authors' knowledge, of CCAAT/enhancer binding protein ( @entity6863 ) cDNA sequence in a fish species. @entity6863 epsilon of @entity2386 was 1861 bp in length (ORF of 822 bp) encoding for 274 @entity1522 , with a calculated molecular weight of 30 kDa. @entity2386 @entity6863 beta was found to be 1561 bp in length (ORF of 1041 bp), encoding for 347 @entity1522 and a calculated molecular weight of 39 kDa. These genes were expressed in various fish organs, tissues and secretions. @entity6863 epsilon was detected by Northern blot from total RNA of head and posterior kidney, heart and spleen. However, RT-PCR also detected @entity6863 epsilon in brain, spleen and peritoneal cavity fluid and peripheral blood leucocyte cDNA. @entity6863 beta was detected by Northern blot analysis in the head and posterior kidney, spleen, intestine, liver, brain, heart, gill and testis and further found by RT-PCR to be detected in mucus, peritoneal cavity fluid, peripheral blood leucocytes and eye cDNA. Phylogenetic analysis placed the @entity2386 @entity6863 beta within the same cluster as previously reported @entity6863 beta sequences. However, @entity2386 @entity6863 epsilon sequence data were not found to cluster with the three reported @entity1 @entity6863 epsilon sequences currently available. Understanding @entity6863 transcriptional gene control in commercially important fish species may lead to a better control of disease.

Molecular cloning and expression of CCAAT/enhancer binding proteins in XXXX @entity2386 .

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity2386 :: ('8255', 'Species') :: ['Japanese flounder'] @entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity6863 :: ('1050', 'Gene') :: ['C/EBP']
@entity2386 :: (8255,Species) :: ['Japanese flounder']
