PURPOSE: The aim of this study was to establish a simple tool to predict good-quality embryos in @entity52 ( @entity52 ) by using cumulus cells (CCs) or peripheral blood cells (PBCs). METHODS: Mitochondrial DNA was extracted from CCs and PBCs in @entity1 undergoing @entity52 . Using real-time polymerase chain reaction, mtDNA copy number in a single cell was calculated. Embryo quality was assessed when it was transferred or frozen. RESULTS: CCs were obtained from 60 oocyte cumulus-cell complexes (OCCCs) in 30 @entity1 , and PBCs were collected from 18 @entity1 . For the 30 @entity1 in the study, the median age was 37  years old (range, 24-43), and the mean body mass index was 21.4 (standard error, 2.0). mtDNA content of CCs and PBCs was highly correlated (Pearson's r   =   0.900, p   <   0.0001). The median mtDNA content of CCs for good- and poor-quality embryos was 140 and 57, respectively (p   <   0.0001). The median mtDNA content of PBCs for good- and poor-quality embryos was 36 and 13, respectively (p   =   0.604). The logistic regression model indicated that mtDNA content in CCs was the only parameter that predicted good-quality embryos (p   =   0.020). The receiver operating characteristic curve for obtaining good-quality embryos by mtDNA copy number in CCs had an area under the curve of 0.823, and using a threshold of 86, positive and negative predictive values were 84.4 and 82.1  %, respectively. CONCLUSIONS: The determination of mtDNA content in CCs can be used to predict good-quality embryos.

Mitochondrial DNA copy number in cumulus cells is a strong predictor of obtaining good-quality embryos after XXXX .

@entity52 :: ('MESH:C566179', 'Disease') :: ['IVF', 'in vitro fertilization'] @entity1 :: ('9606', 'Species') :: ['patients', 'women']
@entity52 :: (MESH:C566179,Disease) :: ['IVF']
