Even with current promising antitumor antibodies, their antitumor effects on stroma-rich solid @entity5 have been insufficient. We used mild @entity221 with the intent of improving drug delivery by breaking the stromal barrier. Here, we provide preclinical evidence of cetuximab + mild @entity221 therapy. We used four in vivo @entity788 xenograft @entity19 models with different stroma amounts (scarce, MIAPaCa-2; moderate, BxPC-3; and abundant, Capan-1 and Ope-xeno). Cetuximab (1 mg/kg) was given systemically, and the @entity19 leg @entity5 were concurrently heated using a water bath method for 30 min at three different temperatures, 25  C (control), 37  C (intra-abdominal organ level), or 41  C (mild @entity221 ) (n = 4, each group). The evaluated variables were the antitumor effects, represented by @entity5 volume, and in vivo cetuximab accumulation, indirectly quantified by the immunohistochemical fluorescence intensity value/cell using antibodies against human IgG Fc. At 25  C, the antitumor effects were sufficient, with a cetuximab accumulation value (florescence intensity/cell) of 1632, in the MIAPaCa-2 model, moderate (1063) in the BxPC-3 model, and negative in the Capan-1 and Ope-xeno models (760, 461). By applying 37  C or 41  C heat, antitumor effects were enhanced shown in decreased @entity5 volumes. These enhanced effects were accompanied by boosted cetuximab accumulation, which increased by 2.8-fold (2980, 3015) in the BxPC-3 model, 2.5- or 4.8-fold (1881, 3615) in the Capan-1 model, and 3.2- or 4.2-fold (1469, 1922) in the Ope-xeno model, respectively. Cetuximab was effective in treating even stroma-rich and k-ras mutant @entity788 @entity19 models when the drug delivery was improved by combination with mild @entity221 .

Cetuximab delivery and antitumor effects are enhanced by mild XXXX in a xenograft @entity19 model of @entity788 .

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancers', 'tumors'] @entity788 :: ('MESH:D010190', 'Disease') :: ['pancreatic cancer'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity221 :: ('MESH:D005334', 'Disease') :: ['hyperthermia']
@entity221 :: (MESH:D005334,Disease) :: ['hyperthermia']
