@entity273 ( @entity274 ), an oxidant generated by inflammatory cells, is an important mediator of injury of endothelial cells (ECs). Here we show that @entity274 induces up-regulation of the expression of Fas, a death signal, in @entity1 ECs in culture. Flow cytometric analysis with a mAb against @entity1 Fas showed that incubation for 24 h with @entity274 induced a dose-dependent increase in the level of Fas in ECs. Coincubation with catalase, which rapidly degrades @entity274 , inhibited @entity274 -induced up-regulation of Fas. @entity274 also induced a dose-dependent increase in Fas mRNA level. A significant increase in Fas mRNA levels was observed from 6 h after stimulation with @entity274 . @entity8870 , a protein phosphatase inhibitor, significantly enhanced Fas mRNA and protein levels in @entity274 -treated ECs. On the other hand, @entity670 , a @entity669 kinase inhibitor, inhibited @entity274 -induced Fas mRNA expression. Furthermore, a flow cytometric method with @entity2667 staining and electron microscopic analysis showed that incubation with an agonistic Ab against Fas (anti-Fas IgM) induced apoptosis in @entity274 -treated cells. These findings suggest that @entity274 induces up-regulation of Fas in ECs and that activation of protein @entity669 kinase may be involved in the mechanism of @entity274 -induced Fas expression. Therefore, Fas-mediated apoptosis may have a pathologic role in @entity274 -induced @entity130 and thereby provide a new therapeutic target.

XXXX induces up-regulation of Fas in @entity1 endothelial cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H2O2'] @entity670 :: ('MESH:D019833', 'Chemical') :: ['genistein'] @entity2667 :: ('MESH:D011419', 'Chemical') :: ['propidium iodide'] @entity130 :: ('MESH:D014947', 'Disease') :: ['EC injury'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['Hydrogen peroxide'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine'] @entity8870 :: ('MESH:D014638', 'Chemical') :: ['Vanadate']
@entity273 :: (MESH:D006861,Chemical) :: ['Hydrogen peroxide']
