Juvenile hormone @entity27716 kinase (JHDK) is an enzyme involved in @entity12808 degradation. In the present article, a putative JHDK cDNA (LdJHDK) was cloned from the Colorado @entity415 beetle Leptinotarsa decemlineata. The cDNA consists of 814 bp, containing a 555 bp open reading frame encoding a 184 @entity3689 protein. LdJHDK reveals a high degree of identity to the previously reported insect JHDKs. It possesses three conserved purine @entity4334 -binding elements, and contains three EF-hand motifs (helix-loop-helix structural domains). LdJHDK mRNA was mainly detected in hindgut and Malpighian tubules. Besides, a trace amount of LdJHDK mRNA was also found in thoracic muscles, brain-corpora cardiaca-corpora allata complex, foregut, midgut, ventral ganglia, fat body, epidermis, and hemocytes. Moreover, LdJHDK was expressed throughout all developmental stages. Within the first, second, and third larval instar, the expression levels of LdJHDK were higher just before and right after the molt, and were lower in the intermediate instar. In the fourth larval instar, the highest peak of LdJHDK occurred 56 h after ecdysis. Ingestion of double-stranded RNA (dsRNA) against LdJHDK successfully knocked down the target gene, increased @entity12808 titer, and significantly upregulated LdKr-h1 mRNA level. Knockdown of LdJHDK significantly impaired adult emergence. Thus, we provide a line of experimental evidence in L. decemlineata to support that LdJHDK encodes function protein involved in @entity12808 degradation.

CHARACTERIZATION AND FUNCTIONAL STUDY OF A PUTATIVE JUVENILE HORMONE DIOL KINASE IN THE COLORADO XXXX BEETLE Leptinotarsa decemlineata (Say).

@entity415 :: ('4113', 'Species') :: ['potato'] @entity12808 :: ('MESH:C566556', 'Disease') :: ['JH'] @entity4334 :: ('D009711', 'Chemical') :: ['nucleotide'] @entity3689 :: ('D000596', 'Chemical') :: ['amino acid'] @entity27716 :: ('CHEBI:23824', 'Chemical') :: ['diol']
@entity415 :: (4113,Species) :: ['POTATO']
