Molecular epizootiology of piroplasmids ( @entity743 spp., Theileria spp.) and @entity14990 was studied in mammals from southern Europe (mainly from Spain, but also from Portugal and France). Partial amplification and sequencing of the 18s rRNA gene was used for molecular diagnosis. In some particular cases ( @entity744 and @entity14097 ) the complete 18s rRNA gene was sequenced. Blood samples were taken from domestic animals showing clinical symptoms: 10 @entity235 , 10 @entity1641 , 10 @entity291 , 9 @entity742 and 1 @entity1237 . In addition, DNA samples were isolated from blood of 12 healthy @entity235 and from spleen of 10 wild red foxes ( @entity13616 ). The results of the survey were the following: Piroplasmid infections: Approximately from 50 to 70% of wild or domestic mammals (symptomatic) were infected. Piroplasmids detected in ruminants were: @entity291 : @entity14097 , T. annulata and Theileria sp. (type C). @entity742 and @entity1237 : @entity744 . Piroplasmids present in canids were: @entity743 canis vogeli, @entity743 @entity235 , Theileria annae and B. equi. The only piroplasmid found in asymptomatic @entity235 was B. equi. Piroplasmids found in @entity1641 were: B. equi and @entity14991 canis. @entity14991 infections in canids: @entity14991 was absent of domestic @entity235 samples, whereas all foxes studied were infected by this protozoa. Genetic analysis showed that most of piroplasmid and Hepatozoon isolates from southern Europe matched unambigously with previously described species, as demonstrated by the high level sequence identity between them, usually between 99 and 100%. Minor differences, usually detected in hypervariable regions of 18s rRNA gene are probably due to strain variations or rare genetic polymorphisms. A possible exception was @entity14097 , which shows a relatively lower degree of homology (94%) with regard to other @entity14097 isolates from several countries. The same is true for @entity744 , that showed a 94% identity with regard to @entity743 sp. from South African @entity291 and a 92% with rapport to @entity14097 from Portugal.

Molecular studies on XXXX , Theileria and Hepatozoon in southern Europe. Part I. Epizootiological aspects.

@entity1641 :: ('9796', 'Species') :: ['horses', 'horse'] @entity742 :: ('9940', 'Species') :: ['sheep', 'Sheep'] @entity235 :: ('9615', 'Species') :: ['canis canis', 'dog', 'dogs'] @entity13616 :: ('9627', 'Species') :: ['Vulpes vulpes'] @entity14991 :: ('136241', 'Species') :: ['B. canis', 'H. canis'] @entity14990 :: ('MESH:C531834', 'Disease') :: ['Hepatozoon canis'] @entity744 :: ('236', 'Species') :: ['B. ovis'] @entity743 :: ('MESH:D001404', 'Disease') :: ['Babesia'] @entity291 :: ('9913', 'Species') :: ['cows', 'cow', 'COW'] @entity1237 :: ('9925', 'Species') :: ['goat'] @entity14097 :: ('5865', 'Species') :: ['B. bovis']
@entity743 :: (MESH:D001404,Disease) :: ['Babesia']
