UNASSIGNED: e21053 Background: In the majority of cases metastases show an organ-specific pattern of spread. The soil theory states that different organs have special abilities to arrest, attract, and promote specific types of @entity5 cells. In the seed concept @entity5 cells co-opt chemokine-mediated signalling (seed-factor). We investigated the expression of the chemokine receptors [CR] on circulating @entity5 cells [CTC] in a cohort of @entity1 with solid @entity5 . METHODS: Twenty millilitre blood were collected after informed consent from 68 consecutive @entity1 affected by @entity320 [ @entity320 ] (n=29) or @entity189 [ @entity189 ] (n=39). Blood was enriched for @entity5 cells by @entity1195 depletion using a magnetic bead separation technique. Potential CTC were identified using flow cytometry with the surface marker EpCAM and intracellular cytokeratin 7-8 ( @entity189 specimens) or @entity320 surface chondroitin sulphate proteoglycan ( @entity320 specimes). Multiparameter cytometry assessed surface expression of the combinations of @entity436 and @entity14284 and for @entity320 specimens also @entity9350 and @entity26245 . RESULTS: Forty-nine @entity1 (71%) [28 @entity189 ; 21 @entity320 ] showed presence of CTC. The median number of CTC was 3 (range: 1-94)/10mL [ @entity189 : 2 (range: 1-21)/10mL; @entity320 4 (range: 1-94)/10mL]. Median number of CR-positive cells and positivity rates are depicted in the table. Altogether @entity436 was significantly higher expressed on CTC than @entity14284 (p = 0.015). Expression rates of @entity436 and @entity14284 did not significantly differ between @entity189 and @entity320 . No correlation was observed between expression of CR on CTC and presence of liver or lung metatastasis. The median number of cells negative for expression of CR was significantly higher compared to the median number of cells positive for at least one of the CR examined (p=0.013). CONCLUSIONS: CTC expressed the CR @entity436 , @entity14284 , @entity9350 and @entity26245 . A correlation between expression of CR and metastatic pattern was not observed and a consistent number of CTC did not express the CR we investigated. [Table: see text].

Analysis of XXXX expression on circulating @entity5 cells (CTC) in @entity1 with solid @entity5 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'tumors', 'tumour'] @entity9350 :: ('1236', 'Gene') :: ['CCR7'] @entity1195 :: ('5788', 'Gene') :: ['CD45'] @entity189 :: ('MESH:D002277', 'Disease') :: ['C', 'carcinoma'] @entity26245 :: ('10803', 'Gene') :: ['CCR9'] @entity14284 :: ('1235', 'Gene') :: ['CCR6'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma', 'M', 'metastatic melanoma'] @entity436 :: ('7852', 'Gene') :: ['CXCR4']
@entity436 :: (7852,Gene) :: ['chemokine receptor']
