The localization of caspases and their substrates in different cellular compartments may be one way to regulate apoptosis. @entity522 -dependent proteolysis of inhibitor @entity13779 ( @entity15975 ) activates @entity13779 (CAD), which induces apoptotic internucleosomal DNA degradation. The nuclear localization of @entity15975 , pro- and active- @entity522 molecules remains a controversial issue. Using a combination of immunodetection of endogenous molecules and confocal microscopy, we analysed the kinetics of the @entity522 and CAD activation induced by @entity24344 triggering in Jurkat cells. Through a semi-quantitative image analysis, we showed a constitutive nuclear localization of @entity522 and @entity15975 in non-apoptotic cells. @entity24344 stimulation induced 7A6 apoptotic antigen expression, which could be related to three different sequential patterns of nuclear chromatin organization. Active- @entity522 first appeared in the cytoplasm and was next observed in the nucleus. Simultaneously, the amount of @entity15975 located in the nucleus decreased, whereas the amount of @entity15975 located in the cytoplasm remained unchanged. Thus, our experiments using in situ immunodetection of endogenous molecules show that the @entity15975 cleavage by the active- @entity522 probably takes place in the nucleus. These results provide new perspectives about the subcellular compartmentation and traffic of caspases during the apoptotic process.

Constitutive nuclear localization and initial cytoplasmic apoptotic activation of endogenous XXXX evidenced by confocal microscopy.

@entity13779 :: ('1677', 'Gene') :: ['caspase-activated deoxyribonuclease'] @entity24344 :: ('MESH:C535950', 'Disease') :: ['FAS'] @entity522 :: ('836', 'Gene') :: ['procaspase-3', 'pro-caspase 3', 'caspase-3', 'Caspase-3'] @entity15975 :: ('1676', 'Gene') :: ['ICAD']
@entity522 :: (836,Gene) :: ['caspase-3']
