@entity11502 (ANG II)-induced interleukin (IL)-6 synthesis requires @entity1110 -oxidase-derived @entity224 anions. Since @entity1110 -oxidase activation by cytokines involves @entity12032 (LOX)-derived @entity8285 ( @entity8285 ) formation, we postulated that @entity8285 is involved in the ANG II-dependent @entity1110 -oxidase activation. Therefore, 5-LOX expression and LTB(4) formation following ANG II (100nM) stimulation were determined in @entity35 aortic smooth muscle cells (SMC). Reactive @entity26 species (ROS)-formation and @entity568 mRNA expression were analyzed following ANG II and @entity8285 (0.6 microM) stimulation. 5-LOX mRNA and protein were detected in SMC. ANG II-induced @entity8285 formation at 2.5min and was followed by an increase in ROS-formation and @entity568 mRNA expression. Blockade of 5-LOX by @entity20463 (200nM) abrogated @entity8285 -formation, ROS-formation, and @entity568 mRNA expression. Moreover, @entity8285 -induced ROS-formation and @entity568 mRNA expression was abolished by @entity1110 -oxidase inhibition using @entity3056 (DPI 10 microM). In conclusion, the present study demonstrates that ANG II enhances LTB(4)-formation in an 5-LOX dependent manner. @entity8285 activates the vascular type @entity1110 -oxidase, leading to an increase in @entity568 transcripts.

XXXX is involved in the angiotensin II-induced @entity1110 -oxidase activation.

@entity8285 :: ('MESH:D007975', 'Chemical') :: ['leukotriene B(4)', 'LTB(4)'] @entity20463 :: ('MESH:C060893', 'Chemical') :: ['MK886'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity12032 :: ('25290(Tax:10116)', 'Gene') :: ['5-lipoxygenase'] @entity568 :: ('24498(Tax:10116)', 'Gene') :: ['IL-6'] @entity1110 :: ('MESH:D009249', 'Chemical') :: ['NAD(P)H'] @entity3056 :: ('MESH:C007517', 'Chemical') :: ['diphenyleneiodonium chloride'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity224 :: ('MESH:D013481', 'Chemical') :: ['superoxide'] @entity11502 :: ('MESH:D000804', 'Chemical') :: ['Angiotensin II']
@entity12032 :: (25290(Tax:10116),Gene) :: ['5-Lipoxygenase']
