AIMS: In the era of potentially disease modifying agents like JAK inhibitors, accurate grading and differentiation of bone marrow (BM) @entity82 has become more relevant to assess staging of disease and therapeutic effects. However, different @entity82 grading models were used in the past without uniformity including the proposal by the World Health Organization. Current scoring systems are based only on reticulin @entity82 . Therefore additional assessment of collagen and the grade of @entity16455 appear to be essential to discriminate all components of the complex BM fibrous matrix. METHODS AND RESULTS: We evaluated problems and pitfalls regarding staining techniques and the interpretation of reticulin @entity82 on a total of 352 samples. Furthermore, we propose a minor modification of the current grading, and separate scoring for collagen deposition and @entity16455 . Reproducibility of gradings was tested among 11 haematopathologists in a blinded assessment. Overall the inter-rater reliability of all three grading systems ranged between 0.898 and 0.926. CONCLUSIONS: standardized assessment of BM @entity82 with differentiation between reticulin, collagen, and @entity16455 is recommended to evaluate the various components of the fibrous matrix which may be delinked after therapy. In this regard quality of staining and application of laboratory standards enable a highly reproducible scoring. This article is protected by copyright. All rights reserved.

Problems and pitfalls in grading of bone marrow fibrosis, collagen deposition and XXXX - a consensus based study.

@entity16455 :: ('MESH:D010026', 'Disease') :: ['osteosclerosis'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis']
@entity16455 :: (MESH:D010026,Disease) :: ['osteosclerosis']
