Devrieseasis caused by Devriesea agamarum is a highly prevalent disease in captive desert lizards, resulting in severe @entity1909 and in some cases mass mortality. In this study, we assessed the contribution of autovaccination to devrieseasis control by evaluating the capacity of 5 different @entity406 -inactivated D. agamarum vaccines to induce a humoral immune response in bearded dragons ( @entity34450 ). Each vaccine contained one of the following adjuvants: CpG, incomplete Freund's, Ribi, @entity12525 , or curdlan. Lizards were administrated one of the vaccines through subcutaneous injection and booster vaccination was given 3 weeks after primo-vaccination. An indirect ELISA was developed and used to monitor lizard serological responses. Localized adverse effects following subcutaneous immunization were observed in all but the Ribi adjuvanted vaccine group. Following homologous experimental challenge, the incomplete Freund's as well as the Ribi vaccine were observed to confer protection in bearded dragons against the development of D. agamarum associated @entity295 but not against @entity1909 . Subsequently, two-dimensional gelelectrophoresis followed by immunoblotting and mass spectrometry was conducted with serum obtained from 3 lizards that showed seroconversion after immunisation with the Ribi vaccine. Fructose-bisphosphate aldolase and aldo-keto reductase of D. agamarum reacted with serum from the latter lizards. Based on the demonstrated seroconversion and partial protection against D. agamarum associated disease following the use of @entity406 -inactivated vaccines as well as the identification of target antigens in Ribi vaccinated bearded dragons, this study provides promising information towards the development of a vaccination strategy to control devrieseasis in captive lizard collections.

Autovaccination Confers Protection against Devriesea agamarum Associated @entity295 but Not XXXX in Bearded Dragons ( @entity34450 ).

@entity295 :: ('MESH:D018805', 'Disease') :: ['septicemia'] @entity406 :: ('MESH:D005557', 'Chemical') :: ['formalin'] @entity34450 :: ('103695', 'Species') :: ['Pogona vitticeps'] @entity12525 :: ('CHEBI:33130', 'Chemical') :: ['aluminium hydroxide'] @entity1909 :: ('MESH:D003872', 'Disease') :: ['dermatitis']
@entity1909 :: (MESH:D003872,Disease) :: ['Dermatitis']
