The constitutive and the heat-shock-induced expression of members of heat-shock protein families changed during vegetative development and conidiation of @entity1980 as determined by two-dimensional gel electrophoresis. Western blot and ELISA analyses revealed the highest amounts of the constitutive heat-shock protein 70 (HSC70) in conidiating aerial hyphae and dormant conidia. During conidial germination the amount of HSC70 decreased and subsequently increased during vegetative growth. Stationary mycelia and young aerial hyphae exhibited the lowest HSC70 level. The stationary-phase-dependent decrease in HSC70 was accompanied by a concomitant increase in its nuclear localization, whereas no significant changes in the amount of nuclear HSC70 were found during aerial hyphae development. The @entity268 content during aerial hyphae development was inversely correlated with that of HSC70. To examine possible causal relations between HSC70 expression and @entity268 content, the @entity1920 -cyclase-deficient mutant crisp (cr-1) was analysed, which exhibits low concentrations of endogenous @entity268 . This mutant, however, showed a lower constitutive HSC70 level, compared to the bdA strain. Treatment of the bd strain and cr-1 mutant with 20 microM @entity5987 did not result in significant changes of the constitutive HSC70 level, but in the level of heat-induced HSC/HSP70. In a developmental mutant (acon-2) which is defective in a differentiation step toward conidiation, the expression of HSC70 in aerial hyphae was delayed until the first proconidial chains were observed. It is concluded that the differential expression of HSC/HSP70 does not depend on different nuclear levels of HSC70 or on changes in @entity268 concentrations, but rather on developmental genes controlling conidiation.

Differential HSC70 expression during asexual development of XXXX .

@entity1980 :: ('5141', 'Species') :: ['Neurospora crassa'] @entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity1920 :: ('CHEBI:16027', 'Chemical') :: ['adenylate'] @entity5987 :: ('MESH:D015124', 'Chemical') :: ['8-bromo-cAMP']
@entity1980 :: (5141,Species) :: ['Neurospora crassa']
