A method of plant culture was developed for growing large leaves of glandless @entity2708 on single stems. Chloroplasts isolated from these leaves actively reduced @entity9588 when assayed for the Hill reaction. Hill reaction activity increased 133% when the 0.5 m @entity969 isolation medium was replaced with 10% (w/v) @entity968 , both buffered at pH 7.6. The presence of 2 or 5% (w/v) bovine serum albumin in the @entity969 buffer did not increase Hill activity. @entity9588 reduction in the dark occurred in all assays, and the possibility of @entity6319 as the reductant is discussed. Half-life of the chloroplasts stored in 10% @entity653 at -23 C was 23 days. The @entity3104 ion at 0.01 m enhanced Hill reaction activity up to 171%. Leaves containing chloroplasts with the highest Hill reaction activity were found near the 8th node below the apex. Leaf water potentials less than -28 bars reduced the activity about 50%. Daylight conditions during the winter months in the greenhouse reduced the activity about 30%.

Some factors affecting the Hill reaction activity in XXXX chloroplasts.

@entity2708 :: ('3635', 'Species') :: ['cotton'] @entity6319 :: ('MESH:D006072', 'Chemical') :: ['gossypol'] @entity653 :: ('MESH:D005990', 'Chemical') :: ['glycerol'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity3104 :: ('MESH:D000644', 'Chemical') :: ['ammonium'] @entity9588 :: ('MESH:C007931', 'Chemical') :: ['Ferricyanide', 'ferricyanide'] @entity968 :: ('MESH:D011092', 'Chemical') :: ['polyethylene glycol']
@entity2708 :: (3635,Species) :: ['cotton']
