OBJECTIVE: To determine effects of @entity4823 and @entity1281 on @entity235 chondrocytes in 3-dimensional culture. SAMPLE POPULATION: Chondrocytes isolated from articular cartilage of 2 adult female @entity235 recently euthanatized for reasons unrelated to @entity854 . PROCEDURE: Chondrocytes were cultured in a 3-dimensional agarose-based medium alone (control), with @entity4823 (100 microg/ml; GL), or with @entity1281 (18 microg/ml; @entity5427 ). Supernatant and agarose plugs from 4 wells/group/d were collected on days 3, 6, and 12 of culture. Agarose plugs were evaluated for percentage of viable cells, percentage of cells producing pericellular or territorial matrix, glycosaminoglycan (GAG) concentration, and @entity22609 production. @entity1421 concentration in supernatants was determined. RESULTS: Chondrocytes in all groups had characteristics indicative of viability and differentiation; however, on day 12, a lower percentage of viable cells was detected in the @entity5427 group, compared with the other 2 groups. On day 6, GAG concentration in the @entity5427 group was significantly greater than concentrations in the other 2 groups. On day 12, GAG concentrations in the GL and @entity5427 groups were significantly less than in the control group. Within the GL and @entity5427 groups, cell viability was significantly less on day 12, compared with day 3. Significant differences in @entity1421 concentration among or within groups and evidence of @entity22609 production were not detected. CONCLUSIONS: 3-dimensional culture of @entity235 chondrocytes allows for production of hyaline cartilage matrix constituents and growth of cells with morphologic characteristics similar to those of articular cartilage. @entity1281 and @entity4823 , at the single concentration evaluated, had detrimental effects on chondrocyte viability, GAG production, or both.

In vitro effects of @entity4823 and @entity1281 on XXXX chondrocytes in three-dimensional culture.

@entity5427 :: ('MESH:D017204', 'Disease') :: ['AS'] @entity235 :: ('9615', 'Species') :: ['canine', 'dogs'] @entity1421 :: ('MESH:D015232', 'Chemical') :: ['PGE2', 'Prostaglandin E2'] @entity4823 :: ('MESH:D005944', 'Chemical') :: ['glucosamine'] @entity22609 :: ('403826(Tax:9615)', 'Gene') :: ['type-II collagen', 'type II collagen'] @entity854 :: ('MESH:D018376', 'Disease') :: ['orthopedic abnormalities'] @entity1281 :: ('CHEBI:15365', 'Chemical') :: ['acetylsalicylate', 'Acetylsalicylate']
@entity235 :: (9615,Species) :: ['canine']
