BACKGROUND: Pancreatic stellate cells (PSCs) play a critical role in @entity1124 ( @entity1124 ). Activated PSCs are the main source of @entity82 in @entity1689 in @entity1124 . The majority of studies on @entity991 are based on in vitro experiments relying on immortalized cell lines derived from diseased @entity1 @entity788 or from animal models. These PSCs are usually activated and may not represent the biological context of their tissue of origin. PURPOSE: (1) To isolate and culture primary @entity1 @entity991 from different disease contexts with minimal impact on their state of activation. (2) To perform a comparative analysis of phenotypes of @entity991 derived from different contexts. METHODS: PSCs were isolated from normal @entity788 , chronic @entity788 , and @entity1124 using a hybrid method of digestion and outgrowth. To minimize activation by serum compounds, cells were cultured in a low-serum environment (2.5  % fetal bovine serum (FBS)). Expression patterns of commonly used markers for @entity991 phenotype and activity were compared between primary @entity991 lines derived from different contexts and correlated to expression in their original tissues. RESULTS: Isolation was successful from 14 of 17 tissues (82  %). @entity991 displayed stable viability and phenotype in low-serum environment. Expression profiles of isolated @entity991 and matched original tissues were closely correlated. @entity1124 -derived @entity991 tended to have a higher status of activation if compared to @entity991 derived from non-cancerous tissues. CONCLUSIONS: Primary @entity1 PSCs isolated from different contexts and cultured in a low-serum environment maintain a phenotype that reflects the stromal activity present in their tissue of origin.

Isolation and culture of primary XXXX pancreatic stellate cells that reflect the context of their tissue of origin.

@entity1 :: ('9606', 'Species') :: ['human'] @entity788 :: ('MESH:D010190', 'Disease') :: ['pancreatitis', 'pancreas'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis'] @entity1124 :: ('MESH:D021441', 'Disease') :: ['PDAC', 'pancreatic ductal adenocarcinoma'] @entity991 :: ('MESH:D015209', 'Disease') :: ['Isolated PSC', 'PSC'] @entity1689 :: ('MESH:D050500', 'Disease') :: ['chronic pancreatitis and of desmoplasia']
@entity1 :: (9606,Species) :: ['human']
