UNASSIGNED: Numerous hypotheses of temporal lobe epileptogenesis have been proposed, and several involve hippocampal mossy cells. Building on previous hypotheses we sought to test the possibility that after @entity130 surviving mossy cells develop into super-connected @entity410 -generating hub cells. If so, they might require more cellular machinery and consequently have larger somata, elongate their dendrites to receive more synaptic input, and display higher frequencies of miniature excitatory synaptic currents (mEPSCs). To test these possibilities @entity2134 -treated @entity19 were evaluated using @entity9449 -immunocytochemistry, whole-cell recording, and @entity18663 -labeling. @entity302 @entity2134 -treated @entity19 displayed substantial loss of @entity9449 -positive hilar neurons. Somata of surviving neurons were 1.4-times larger than in controls. @entity18663 -labeled mossy cells also were larger in @entity302 @entity19 , but dendritic length per cell was not significantly different. The average frequency of mEPSCs of mossy cells recorded in the presence of @entity1022 and @entity1027 was 3.2-times higher in @entity302 @entity2134 -treated @entity19 as compared to controls. Other parameters of mEPSCs were similar in both groups. Average input resistance of mossy cells in @entity302 @entity19 was reduced to 63% of controls, which is consistent with larger somata and would tend to make surviving mossy cells less excitable. Other intrinsic physiological characteristics examined were similar in both groups. Increased excitatory synaptic input is consistent with the hypothesis that surviving mossy cells develop into aberrantly super-connected @entity410 -generating hub cells, and soma @entity1184 is indirectly consistent with the possibility of axon sprouting. However, no obvious evidence of hyperexcitable intrinsic physiology was found. Furthermore, similar @entity1184 and @entity2597 has been reported for other neuron types in the dentate gyrus, suggesting mossy cells are not unique in this regard. Thus, findings of the present study reveal @entity302 -related changes in mossy cell anatomy and synaptic input but do not strongly support the hypothesis that mossy cells develop into @entity410 -generating hub cells.    2014 Wiley Periodicals, Inc.

Surviving mossy cells enlarge and receive more excitatory synaptic input in a XXXX model of temporal lobe epilepsy.

@entity410 :: ('MESH:D012640', 'Disease') :: ['seizure'] @entity2597 :: ('MESH:D003240', 'Disease') :: ['hyper-connectivity'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity1027 :: ('MESH:D001640', 'Chemical') :: ['bicuculline'] @entity1022 :: ('MESH:D013779', 'Chemical') :: ['tetrodotoxin'] @entity1184 :: ('MESH:D006984', 'Disease') :: ['hypertrophy'] @entity2134 :: ('MESH:D010862', 'Chemical') :: ['pilocarpine'] @entity18663 :: ('CHEBI:27870', 'Chemical') :: ['biocytin', 'Biocytin'] @entity302 :: ('MESH:D004827', 'Disease') :: ['epileptic', 'Epileptic', 'epilepsy'] @entity130 :: ('MESH:D014947', 'Disease') :: ['epileptogenic injuries'] @entity9449 :: ('14800(Tax:10090)', 'Gene') :: ['GluR2']
@entity19 :: (10090,Species) :: ['mouse']
