Alternative RNA splicing can be regulated in a highly cell- and tissue-specific or developmentally specific manner. In neurons, the functions of many gene products, such as those of @entity26303 genes are regulated by alternative splicing. In this paper the mechanism of neural-specific RNA splicing is investigated using @entity26303 genes as models. First, we confirm the splicing patterns of @entity26303 transcripts during neural differentiation of P19 @entity189 ( @entity189 ) cells. The full-length form of @entity26304 was expressed in the neuronal state. In contrast, both the full-length and truncated forms of @entity26303 C were expressed constitutively in all differentiation states. However, two alternatively spliced forms with either 42- or 117- @entity377 insertions in the @entity669 kinase domain were detected only in the neuronal state. Thus, the expression of functional @entity26304 and C was found to be regulated by alternative splicing during neural differentiation. To examine the molecular basis of neural-specific splicing, and how splicing regulation is modulated in different neurons. The expression of a number of general splicing factors was studied. The mRNA levels of the splicing factors @entity29174 , U2AF SF3a, @entity55672 and @entity65202 was found to decrease rapidly during differentiation. In contrast, Nova, an RNA-binding protein was expressed in the neuronal state. We also found that the levels of two SR proteins, members of a family of splicing factors, increased in the neuronal state. These results suggest that the stoichiometric balance among some splicing factors, including SR proteins, may be associated with the alternative splicing of @entity26303 transcripts during differentiation.

Changes in pre-mRNA splicing factors during neural differentiation in P19 XXXX cells.

@entity26303 :: ('18211(Tax:10090)', 'Gene') :: ['trk'] @entity377 :: ('CHEBI:36976', 'Chemical') :: ['nucleotide'] @entity189 :: ('MESH:D002277', 'Disease') :: ['embryonal carcinoma', 'EC'] @entity55672 :: ('53610(Tax:10090)', 'Gene') :: ['p54nrb'] @entity29174 :: ('110809(Tax:10090)', 'Gene') :: ['ASF/SF2'] @entity65202 :: ('19205(Tax:10090)', 'Gene') :: ['PTB'] @entity26304 :: ('18212(Tax:10090)', 'Gene') :: ['trk B'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine']
@entity189 :: (MESH:D002277,Disease) :: ['embryonal carcinoma']
