UNASSIGNED: Mesoporous @entity3440 -based particles are promising candidates for biomedical applications. Here, we address the importance of macrophage activation status for internalization of AMS6 (approx. 200  nm in diameter) versus AMS8 (approx. 2   m) mesoporous @entity3440 particles and the role of different phagocytosis receptors for particle uptake. To this end, @entity2823 -conjugated @entity3440 particles were used. AMS8 were found to be non-cytotoxic both for @entity6824 -stimulated (anti-inflammatory) and @entity2841 -stimulated (pro-inflammatory) macrophages, whereas AMS6 exhibited @entity137 towards @entity6824 -stimulated, but not @entity2841 -stimulated macrophages; this @entity137 was, however, mitigated in the presence of serum. AMS8 triggered the secretion of pro-inflammatory cytokines in @entity6824 -activated cells. Class A scavenger receptor ( @entity13242 ) expression was noted in both @entity6824 and @entity2841 -stimulated macrophages, although the expression was higher in the former case, and gene silencing of @entity13242 resulted in a decreased uptake of AMS6 in the absence of serum. @entity2841 -stimulated macrophages expressed higher levels of the @entity3913 receptor @entity14452 compared to @entity6824 -stimulated cells, and uptake of AMS6, but not AMS8, was reduced following the downregulation of @entity14452 in @entity2841 -stimulated cells; particle uptake was also suppressed by mannan, a competitive ligand. These studies demonstrate that macrophage activation status is an important determinant of particle uptake and provide evidence for a role of different macrophage receptors for cell uptake of @entity3440 particles.

Macrophage activation status determines the internalization of mesoporous XXXX particles of different sizes: Exploring the role of different pattern recognition receptors.

@entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity', 'cytotoxicity'] @entity13242 :: ('4481', 'Gene') :: ['SR-A'] @entity14452 :: ('4360', 'Gene') :: ['CD206'] @entity2823 :: ('MESH:D016650', 'Chemical') :: ['FITC'] @entity3913 :: ('MESH:D008358', 'Chemical') :: ['mannose'] @entity3440 :: ('MESH:D012822', 'Chemical') :: ['silica'] @entity6824 :: ('1435', 'Gene') :: ['M-CSF'] @entity2841 :: ('1437', 'Gene') :: ['GM-CSF']
@entity3440 :: (MESH:D012822,Chemical) :: ['silica']
