We previously identified @entity69437 and @entity38981 as novel topoisomerase I-interacting proteins that share 80% @entity535 identity. Here we report the characterization of their subcellular localization. In a number of @entity19 and @entity1 cells, @entity69437 and @entity38981 ( @entity69438 ) colocalized to punctate or elongated cytoplasmic bodies (< 5 microm long and several per cell) that were larger and more elongated in @entity5 cell lines than in fibroblasts and myoblasts. A search for potential colocalizing proteins identified TRIM family members that localize to morphologically similar cytoplasmic bodies, which were then tested for colocalization with @entity69438 . TRIM5delta, expressed as a GFP fusion, colocalized with @entity69438 immunostaining and appeared to serve as a scaffold for the assembly of endogenous @entity69438 proteins. TRIM family members contain a RING domain, B-box(es), and coiled-coil regions, which have a characteristic order and spacing (RBCC domain). RING-dependent ubiquitin ligase activity and multimerization via the coiled-coil region may be defining properties of the RBCC/TRIM protein family. We found that TRIM5delta with a deleted coiled-coil region or a mutated RING domain failed to colocalize with @entity69438 . Additionally, @entity47960 delta ubiquitylated itself in a RING finger- and @entity69439 -dependent manner. @entity69438 each contain a PHR-similarity region, repeated twice on the putative ubiquitin ligases PAM, highwire and RPM-1, which also contain a RING and B-box. Thus, four protein modules found on each of these putative ubiquitin ligases, a RING, a B-box and two PHR repeats, are present on @entity69438 and @entity47960 delta that are colocalized to cytoplasmic bodies.

@entity69437 and XXXX colocalize to cytoplasmic bodies with the RBCC/tripartite motif protein, TRIM5delta.

@entity1 :: ('9606', 'Species') :: ['human'] @entity69439 :: ('7322', 'Gene') :: ['UbcH5B'] @entity47960 :: ('85363', 'Gene') :: ['TRIM5'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity69437 :: ('53339', 'Gene') :: ['BTBD1'] @entity38981 :: ('84464', 'Gene') :: ['BTBD2'] @entity69438 :: ('84464;53339;55643', 'Gene') :: ['BTBD1/2']
@entity38981 :: (84464,Gene) :: ['BTBD2']
