BACKGROUND: We evaluated three commercially available enzymes for pancreatic digestion by comparing key parameters during the islet isolation process, as well as islet quality post-isolation. METHODS: Retrospectively compared and analyzed islet isolations from pancreata using three different enzyme groups: Liberase HI (n=63), Collagenase @entity15392 (NP) (n=43), and Liberase @entity1 Tissue Free Collagenase/Thermolysin (MTF @entity6344 ) (n=115). A standardized islet isolation and purification method was used. Islet quality assessment was carried out using islet count, viability, in vitro @entity413 -stimulated insulin secretion (GSIS), @entity413 -stimulated @entity26 consumption rate (  OCR), and in vivo transplantation model in @entity19 . RESULTS: Donor characteristics were not significantly different among the three enzyme groups used in terms of age, sex, hospital stay duration, cause of @entity204 , body mass index (BMI), hemoglobin A1c (HbA1c), @entity63 ( @entity63 ), and pancreas weight. Digestion efficacy (percentage of digested tissue by weight) was significantly higher in the Liberase MTF C/T group (73.5    1.5 %) when compared to the Liberase HI group (63.6    2.3 %) (p<0.001) and the Collagenase NB1/NP group (61.7    2.9%) (p<0.001). The stimulation index for GSIS was significantly higher in the Liberase MTF C/T group (5.3    0.5) as compared to the Liberase HI (2.9    0.2) (p<0.0001) and the Collagenase NB1/NP (3.6    2.9) (p=0.012) groups. Furthermore, the Liberase MTF C/T enzymes showed the highest success rate of transplantation in diabetic NOD Scid @entity19 (65%), which was significantly higher than the Liberase HI (42%, p=0.001) and the Collagenase NB1/NP enzymes (41%, p<0.001). CONCLUSIONS: Liberase MTF C/T is superior to Liberase HI and Collagenase NB1/NP in terms of digestion efficacy and @entity413 -stimulated insulin secretion in vitro. Moreover, Liberase MTF C/T had a significantly higher success rate of transplantation in diabetic NOD Scid @entity19 compared to Liberase HI and Collagenase NB1/NP enzymes.

The Choice of Enzyme for XXXX Pancreas Digestion is a Critical Factor for Increasing the Success of Islet Isolation.

@entity1 :: ('9606', 'Species') :: ['Mammalian'] @entity6344 :: ('c|SUB|C||T', 'DNAMutation') :: ['C/T'] @entity63 :: ('MESH:D007511', 'Disease') :: ['CIT', 'cold ischemia time'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity15392 :: ('57126', 'Gene') :: ['NB1/Neutral Protease'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity1 :: (9606,Species) :: ['Human']
