Kunitz-type chymotrypsin inhibitor @entity70353 of @entity12638 @entity12638 is a basic peptide consisting of 62 @entity535 residues. To elucidate the mechanisms of transcriptional regulation of @entity70353 gene expression, we cloned it for genomic structure analysis. @entity70353 cDNA was used as a probe to screen a BAC sub-library. One positive clone containing the upstream sequences was isolated and the sequence result showed that @entity70353 gene consists of three exons spaced by two introns. In the 5'-flanking region, consensus TATA and CCAAT boxes were identified. Other binding sites for transcription factors such as NF-kappaB, GATA, C/EBP, COUP-TF/ @entity70354 , RORalpha1, SRY, and HOXA3 were also detected. Southern blot analysis suggested a single copy of @entity70353 gene in the @entity12638 genome. Northern blot analysis indicated that the expression of @entity70353 gene is transcriptionally regulated during development and is apparently tissue-specific. The @entity70353 mRNA was detected in fat body, ovary, @entity825 , and skin. We furthermore investigated the @entity70353 expression profiles after LPS and @entity526 injection. The fluctuations of @entity70353 transcript in challenged larvae confirm our proposal that @entity70353 is an immune responsible gene. According to our data, we discussed the transcriptional factors putatively responsible for the physiological role of @entity70353 in the @entity12638 hemolymph.

Genomic structure and expression analysis of the gene encoding a XXXX basic Kunitz-type chymotrypsin inhibitor.

@entity825 :: ('MESH:D055090', 'Disease') :: ['trachea'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity70353 :: ('692601(Tax:7091)', 'Gene') :: ['CIb1'] @entity12638 :: ('7091', 'Species') :: ['Bombyx mori', 'silkworm'] @entity526 :: ('562', 'Species') :: ['E. coli'] @entity70354 :: ('693036(Tax:7091)', 'Gene') :: ['HNF-4']
@entity12638 :: (7091,Species) :: ['silkworm']
