ETHNOPHARMACOLOGICAL EVIDENCE: Antrodia camphorata, a highly valued polypore mushroom native only to Taiwan, has been traditionally used as a medicine for anti- @entity32 . AIM OF THE STUDY: In this study, anti-inflammatory effects of Antrodia camphorata (AC) and its active compound, ergostatrien-3b-ol (ST1), were investigated in a @entity19 @entity63 model induced by skin flap surgery on the dorsal skin. MATERIALS AND METHODS: A U-shaped flap was elevated on the dorsal skin of the nine-week-old male @entity19 . @entity19 were randomly assigned to six groups for treatment (n=6) including normal skin/ @entity2771 (PG), surgical skin flap/PG, solid-state-cultured AC (S/AC), wood-cultured AC (W/AC), high-dose ST1 (H-ST1), low-dose ST1 (L-ST1). Antrodia camphorata was dissolved in 25 L PG and smeared on the skin flap every six hours for 24h. At the end of the experiment, each @entity19 was anesthetized, and skin tissues were collected from their back for histopathological analysis, extracting RNA and protein according to our previous reports. RESULTS: Skin-flap-induced @entity63 damage significantly increased the expression of the @entity13633 , @entity10178 , and @entity777 proteins and decreased the expression of @entity10181 protein. In addition, focal, moderate @entity39 with @entity32 was found in the epidermis, and moderate inflammatory cells and @entity39 with slight @entity4 was noted in the sub-dermis at 24h after skin flap surgery. However, treatment with solid-state-cultured or wood-cultured AC, or with its derived ST1 active compound, significantly reduced the @entity39 and @entity32 in both the epidermis and sub-dermis of the skin flap. The treatments also reduced the inflammatory response by decreasing the expression of @entity32 -related genes including @entity13633 , @entity777 , @entity637 , and NF-kB, as shown by changes in RNA and protein expression, when compared with the surgical skin flap procedure alone. CONCLUSIONS: These results demonstrated that methanolic extracts of wood-cultured fruiting bodies and solid-state-cultured mycelia from Antrodia camphorata have excellent anti-inflammatory activities and thus have great potential as an addition for hydrocolloid dressings.

Anti-inflammatory effects of Antrodia camphorata, a herbal medicine, in a @entity19 XXXX model.

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation', 'inflammatory cell infiltration'] @entity2771 :: ('MESH:D019946', 'Chemical') :: ['propylene glycol'] @entity10178 :: ('17709(Tax:10090)', 'Gene') :: ['COX2'] @entity63 :: ('MESH:D007511', 'Disease') :: ['skin ischemia', 'ischemia'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'Mice'] @entity39 :: ('MESH:D009336', 'Disease') :: ['coagulative necrosis', 'necrosis'] @entity4 :: ('MESH:D004487', 'Disease') :: ['edema'] @entity777 :: ('16193(Tax:10090)', 'Gene') :: ['IL-6'] @entity13633 :: ('18126(Tax:10090)', 'Gene') :: ['iNOS'] @entity637 :: ('21926(Tax:10090)', 'Gene') :: ['TNF-a'] @entity10181 :: ('18036(Tax:10090)', 'Gene') :: ['IkB']
@entity63 :: (MESH:D007511,Disease) :: ['skin ischemia']
