As an adjuvant to chemotherapy @entity221 has proven to be successful as a treatment for osteo- and @entity511 @entity1 . The aim of this study was to investigate whether @entity221 could increase cellular expression of heat-shock-protein 72 in @entity1 osteo- and @entity511 cells and how heat treatment would affect their susceptibility to natural killer cell (NK-cell)-mediated lysis. About 5-10% of the peripheral mononuclear blood cells (PBMC) in the @entity1 peripheral blood are natural killer cells (NK-cells). Natural @entity137 , mediated by NK-cells, is believed to play an important role in host defense against @entity5 . The exact mechanisms of recognition of target cells and subsequent NK-cell activation are not yet known. NK-cells, isolated from the peripheral blood of healthy donors, were enriched by magnetic cell-separation to a purity of 85-97%, assessed by FACS-analysis. The susceptibility of heat-treated (42.5 degrees C, 90 minutes) and @entity421 (MG63) and @entity511 (HTB94) cell lines to NK-killing was determined by a release assay of @entity880 dehydrogenase (LDH). Lysis by NK-cells was increased by heat treatment of the target cells from 16.6% + 4.5% to 33% + 15%, p=0.035, for @entity421 cells, (E/T ratio of 5:1) and from 13.7% + 3.1% to 27.9% + 16.9%, p=0.021, (E/T ratio of @entity19 ) for @entity511 cells. An increased expression of @entity1878 of chondro- and @entity421 cells after heat treatment was detected by the Western blot technique. The results of this study show that @entity221 increases @entity1878 expression in osteo- and @entity511 cells and their susceptibility to NK-cell-mediated lysis. These findings may lead to new therapeutic strategies, using @entity221 to improve immunological defense against chondro- and @entity421 cells.

@entity221 increases the susceptibility of chondro- and XXXX cells to natural killer cell-mediated lysis.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity511 :: ('MESH:D002813', 'Disease') :: ['chondrosarcoma'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity19 :: ('10090', 'Species') :: ['20:1'] @entity1878 :: ('3303', 'Gene') :: ['HSP72'] @entity421 :: ('MESH:D012516', 'Disease') :: ['untreated osteosarcoma', 'osteosarcoma'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity221 :: ('MESH:D005334', 'Disease') :: ['hyperthermia']
@entity421 :: (MESH:D012516,Disease) :: ['osteosarcoma']
