OBJECTIVE: Chemokines have a range of biologic activities, including regulation of leukocyte trafficking, modulation of hematopoietic cell proliferation, and adhesion to extracellular matrix molecules. Specifically, @entity14638 ; @entity14638 ; @entity14638 , @entity14638 ) and @entity1570 ( @entity1570 , @entity1570 , @entity1570 ) are chemotactic for @entity1 B cells, and their ligands @entity436 and @entity22750 are differentially expressed on B cells, including malignant B cells. We investigated the expression of these chemokine/chemokine receptors in eyes with primary intraocular @entity1892 (PIOL). DESIGN: Observational case series ( @entity1 tissue study). METHODS: Three freshly enucleated eyes with PIOL and a normal autopsied eye were frozen and sectioned. The sections were evaluated using immunohistochemistry (avidin- @entity1885 -complex immunoperoxidase technique) for @entity436 , @entity22750 , @entity14638 , and @entity1570 to detect the expression and location. Reverse transcriptase-polymerase chain reaction was used to detect chemokine transcripts of @entity436 , @entity22750 , @entity14638 , and @entity1570 in PIOL and @entity989 ( @entity989 ) cells after microdissection-either by laser capture (Arcturus) or by manual dissection-from frozen sections. MAIN OUTCOME MEASURES AND RESULTS: The three PIOL eyes showed similar pathology, with typical diffuse large B- @entity706 cells subjacent to the @entity989 . The eyes also demonstrated a similar chemokine profile. High expression levels of @entity436 and @entity22750 were found limited to the @entity706 cells. In contrast, @entity14638 protein was expressed in the @entity989 but not located in other ocular resident cells. @entity1570 was barely detected in a few @entity989 cells. @entity436 and @entity22750 transcripts were detected abundantly in @entity706 cells, whereas @entity14638 and @entity1570 transcripts were detected only in the @entity989 and not the malignant cells. No chemokine expression was detected on the @entity989 cells in the normal control eye. CONCLUSIONS: Chemokines and chemokine receptors selective for B cells were identified in @entity989 and malignant B cells, respectively. @entity14638 , and possibly @entity1570 , attracts both normal and malignant B-cells while promoting migration of only small numbers of T cells and macrophages. We propose that B-cell chemokines may be involved in the pathogenesis of PIOL by selectively attracting @entity706 cells to the @entity989 from the choroidal circulation. Our data suggest that inhibition of B-cell chemoattractants could be a future strategy for the treatment of PIOL.

Expression of chemokine receptors, @entity436 and XXXX , and chemokines, @entity14638 and @entity1570 , in the eyes of @entity1 with primary intraocular lymphoma.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1885 :: ('MESH:D001710', 'Chemical') :: ['biotin'] @entity706 :: ('MESH:D008223', 'Disease') :: ['lymphoma'] @entity1570 :: ('6387', 'Gene') :: ['CXCL12', 'SCYB12', 'stromal cell-derived factor-1', 'SDF-1'] @entity22750 :: ('643', 'Gene') :: ['CXCR5'] @entity14638 :: ('10563', 'Gene') :: ['CXCL13', 'SCYB13', 'BLC', 'BCA-1', 'B-lymphocyte chemoattractant (BLC)'] @entity1892 :: ('MESH:D016393', 'Disease') :: ['B-cell lymphoma'] @entity989 :: ('MESH:D012164', 'Disease') :: ['retinal pigment epithelium', 'RPE'] @entity436 :: ('7852', 'Gene') :: ['CXCR4']
@entity22750 :: (643,Gene) :: ['CXCR5']
