BACKGROUND: Despite our substantial understanding of molecular mechanisms and gene mutations involved in @entity5 , the technical approaches for diagnosis and prognosis of @entity5 are limited. In routine clinical diagnosis of @entity5 , the procedure is very basic: nuclear morphology is used as a common assessment of the degree of @entity5 , and hence acts as a prognostic and predictive indicator of the disease. Furthermore, though the atypical nuclear morphology of @entity5 cells is believed to be a consequence of oncogenic signaling, the molecular basis remains unclear. Another common characteristic of @entity1 @entity5 is @entity2007 , but the causes and its role in carcinogenesis are not well established. METHODS: We investigated the expression of the nuclear envelope proteins @entity4223 in @entity43 by immunohistochemistry and studied the consequence of @entity4223 suppression using siRNA in primary @entity1 ovarian surface epithelial cells in culture. We used immunofluorescence microscopy to analyze nuclear morphology, flow cytometry to analyze cellular DNA content, and fluorescence in situ hybridization to examine cell ploidy of the @entity4223 -suppressed cells. RESULTS: We found that nuclear lamina proteins @entity4223 are often absent (47%) in @entity43 cells and tissues. Even in @entity4223 -positive @entity43 , the expression is heterogeneous within the population of @entity5 cells. In most @entity5 cell lines, a significant fraction of the @entity4223 -negative population was observed to intermix with the @entity4223 -positive cells. Down regulation of @entity4223 in non-cancerous primary ovarian surface epithelial cells led to morphological deformation and development of @entity2007 . The @entity2007 cells became @entity851 due to a @entity876 -dependent induction of the cell cycle inhibitor @entity3544 . CONCLUSIONS: We conclude that the loss of nuclear envelope structural proteins, such as @entity4223 , may underlie two of the hallmarks of @entity5 in nuclear morphology and @entity2007 .

Nuclear envelope structural defects cause chromosomal numerical instability and XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity43 :: ('MESH:D010051', 'Disease') :: ['ovarian cancer'] @entity2007 :: ('MESH:D000782', 'Disease') :: ['aneuploidy', 'aneuploid'] @entity851 :: ('MESH:D006130', 'Disease') :: ['growth retarded'] @entity4223 :: ('4000', 'Gene') :: ['lamin A/C'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'cancer--aberrations', 'malignancy'] @entity3544 :: ('644914', 'Gene') :: ['p21'] @entity876 :: ('7157', 'Gene') :: ['p53']
@entity43 :: (MESH:D010051,Disease) :: ['aneuploidy in ovarian cancer']
