Although microRNAs (miRNAs) are small, non-protein-coding entities, they have important roles in post-transcriptional regulation of most of the @entity1 genome. These small entities generate fine-tuning adjustments in the expression of mRNA, which can mildly or massively affect the abundance of proteins. Previously, we found that the expression of miR-30c-2-3p is induced by @entity4990 and has an important role in the regulation of cell proliferation in @entity43 cells. The goal here is to confirm that @entity23638 mRNA is a target of miR-30c-2-3p silencing, thereby further establishing the functional role of miR-30c-2-3p. Using a combination of bioinformatics, qRT-PCR, immunoblotting and luciferase assays, we uncovered a regulatory pathway between miR-30c-2-3p and the expression of the transcription factor, @entity23638 . @entity36733 triggers the expression of both miR-30c-2-3p and @entity23638 , which peak at 1 h and are absent 8 h post stimulation in SKOV-3 and @entity1 serous @entity43 cells. The 3  -untranslated region (3  -UTR) of @entity23638 was a predicted, putative target for miR-30c-2-3p, which we confirmed as a bona-fide interaction using a luciferase reporter assay. Specific mutations introduced into the predicted site of interaction between miR-30c-2-3p and the 3  -UTR of @entity23638 alleviated the suppression of the luciferase signal. Furthermore, the presence of anti-miR-30c-2-3p enhanced @entity23638 mRNA and protein after @entity4990 stimulation. Thus, the data suggest that after the expression of @entity23638 and miR-30c-2-3p are elicited by @entity4990 , subsequently miR-30c-2-3p negatively regulates the expression of @entity23638 through post-transcriptional silencing, which prevents further @entity23638 -related outcomes as a consequence of @entity4990 signaling.

XXXX Mediates Activating Transcription Factor 3 Expression Which Is a Target for Post-Transcriptional Silencing by miR-30c-2-3p.

@entity1 :: ('9606', 'Species') :: ['human', 'OVCAR-3'] @entity43 :: ('MESH:D010051', 'Disease') :: ['ovarian cancer'] @entity4990 :: ('MESH:C032881', 'Chemical') :: ['lysophosphatidic acid'] @entity36733 :: ('MESH:D008246', 'Chemical') :: ['Lysophosphatidic acids'] @entity23638 :: ('467', 'Gene') :: ['ATF3']
@entity4990 :: (MESH:C032881,Chemical) :: ['Lysophosphatidic Acid']
