OBJECTIVES: In this study, we have established an in vitro three-dimensional (3D) coculture, where normal endometrial stromal cells and @entity1204 cells were cocultured under defined hormonal conditions, to investigate the potential paracrine effects on synthesis and secretion of matrix metalloproteinases (MMPs) and, thus, @entity3 . METHODS: Endometrial stromal cells were obtained by biopsy, cultured in the presence of 100 nM @entity172 for 3 days, and then mixed with extracellular matrix (ECM) composed of collagen I and matrigel in a 4:1 ratio. After 3 more days in culture, a 3D coculture was established with @entity1 cells, an @entity1204 cell line, grown on top of the mixture under various ovarian @entity230 (no steroid, 100 nM @entity10769 (E2), or 1.0 microM @entity1420 (P4)) for 10 days. The expression and localization of @entity1476 , @entity1495 , and @entity1428 subunit were visualized by immunocytochemistry and analyzed by reverse transcription polymerase chain reaction (RT-PCR). The extent of @entity3 was quantified by Boyden's chamber assay. RESULTS: @entity1428 subunit was localized along the cell surface of @entity1 cell under all three hormonal conditions. @entity1476 was located in the cytoplasm of stromal cells and on the surface of @entity1 cells. Synthesis and secretion of stromal @entity1476 were increased in the presence of ovarian @entity230 . In contrast, expression of stromal @entity1495 was suppressed in the presence of ovarian @entity230 . No MMPs were synthesized in @entity1 cells. Invasion assay revealed that @entity1 cells achieved high tumoral invasiveness in the presence of @entity10769 . CONCLUSIONS: These findings suggest that stromal cell-derived @entity1476 is translocated to the surface of @entity1 cells. @entity1428 subunit might contribute, in part, to providing a binding site for @entity1476 . Thus, @entity1 cells invade by recruiting @entity1476 secreted by stromal cells, which is greatly enhanced in the presence of @entity10769 . The 3D coculture provides an excellent experimental system in which single parameters can be isolated from a complex in vivo system in the process of @entity1204 .

Localization of matrix metalloproteinases on XXXX cell invasion in vitro.

@entity1204 :: ('MESH:D016889', 'Disease') :: ['endometrial adenocarcinoma', 'endometrial cancer', 'endometrial cancer invasion'] @entity1 :: ('9606', 'Species') :: ['HEC-1A'] @entity172 :: ('MESH:D004967', 'Chemical') :: ['estrogen'] @entity1420 :: ('MESH:D011374', 'Chemical') :: ['progesterone'] @entity1476 :: ('4313', 'Gene') :: ['MMP-2'] @entity1428 :: ('3690', 'Gene') :: ['Integrin beta 3', 'integrin beta 3'] @entity3 :: ('MESH:D009362', 'Disease') :: ['cancer invasion'] @entity1495 :: ('4318', 'Gene') :: ['MMP-9'] @entity230 :: ('MESH:D013256', 'Chemical') :: ['steroids'] @entity10769 :: ('CHEBI:16469', 'Chemical') :: ['beta-estradiol']
@entity1204 :: (MESH:D016889,Disease) :: ['endometrial cancer']
