UNASSIGNED: Phospholipase D (PLD) hydrolyses @entity2434 to produce @entity8558 (PA) and @entity1877 . It has two isoforms, @entity67701 and @entity68714 , which are differentially expressed depending on the cell type. In mast cells it plays an important role in signal transduction. The aim of the present study was to clarify the role of @entity68714 in the secretory pathway. @entity35 cells, a mast cell line, transfected to overexpress catalytically active (PLD2CA) and inactive (PLD2CI) forms of @entity68714 were used. Previous observations showed that the Golgi complex was well organized in CA cells, but was disorganized and dispersed in CI cells. Furthermore, in CI cells, the microtubule organizing center was difficult to identify and the microtubules were disorganized. These previous observations demonstrated that @entity68714 is important for maintaining the morphology and organization of the Golgi complex. To further understand the role of @entity68714 in secretory and vesicular trafficking, the role of @entity68714 in the secretory process was investigated. Incorporation of @entity3167 was used to follow the synthesis and transport of glycoconjugates in the cell lines. The modified @entity3167 was subsequently detected by labeling with a fluorophore or @entity1885 to visualize the localization of the molecule after a pulse-chase for various times. Glycoconjugate trafficking was slower in the CI cells and labeled glycans took longer to reach the plasma membrane. Furthermore, in CI cells @entity3167 glycans remained at the plasma membrane for longer periods of time compared to @entity35 cells. These results suggest that @entity68714 activity plays an important role in regulating glycoconjugate trafficking in mast cells.

Phospholipase D2 Modulates the Secretory Pathway in XXXX Mast Cells.

@entity3167 :: ('MESH:D019158', 'Chemical') :: ['sialic acid'] @entity68714 :: ('25097(Tax:10116)', 'Gene') :: ['PLD2'] @entity1885 :: ('MESH:D001710', 'Chemical') :: ['biotin'] @entity2434 :: ('MESH:D010713', 'Chemical') :: ['phosphatidylcholine'] @entity8558 :: ('MESH:D010712', 'Chemical') :: ['phosphatidic acid'] @entity1877 :: ('MESH:D002794', 'Chemical') :: ['choline'] @entity35 :: ('10116', 'Species') :: ['RBL-2H3'] @entity67701 :: ('25096(Tax:10116)', 'Gene') :: ['PLD1']
@entity35 :: (10116,Species) :: ['RBL-2H3']
