UNASSIGNED: @entity340 are usually treated by surgical intervention addressed for the replacement of the damaged valve with a biosynthetic or mechanical prosthesis. Although this approach guarantees a good quality of life for @entity1 , it is not free from drawbacks (structural @entity51 , and reintervention). To overcome these limitations, the @entity340 ( @entity340 ) is developing new strategies to synthesize novel types of valve substitutes, by identifying efficient sources of both ideal scaffolds and cells. In particular, a natural matrix, able to interact with cellular components, appears to be a suitable solution. On the other hand, the well-known Wharton's jelly mesenchymal stem cells (WJ-MSCs) plasticity, regenerative abilities, and their immunomodulatory capacities make them highly promising for @entity340 applications. In the present study, we investigated the possibility to use porcine valve matrix to regenerate in vitro the valve endothelium by WJ-MSCs differentiated along the endothelial lineage, paralleled with @entity1 umbilical vein endothelial cells (HUVECs), used as positive control. Here, we were able to successfully decellularize porcine heart valves, which were then recellularized with both differentiated-WJ-MSCs and HUVECs. Data demonstrated that both cell types were able to reconstitute a cellular monolayer. Cells were able to positively interact with the natural matrix and demonstrated the surface expression of typical endothelial markers. Altogether, these data suggest that the interaction between a biological scaffold and WJ-MSCs allows the regeneration of a morphologically well-structured endothelium, opening new perspectives in the field of @entity340 .

XXXX Mesenchymal Stem Cells Reendothelialize Porcine Heart Valve Scaffolds: Novel Perspectives in Heart Valve Tissue Engineering.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity51 :: ('MESH:D009461', 'Disease') :: ['deterioration, nonstructural dysfunction'] @entity340 :: ('MESH:D006349', 'Disease') :: ['Heart valve diseases', 'HVTE', 'heart valve tissue engineering']
@entity1 :: (9606,Species) :: ['Human']
