Susceptibility testing of fungi by flow cytometry (also called fluorescence-activated cell sorting [FACS]) using vital staining with @entity2937 showed a good correlation with the standard M27-A procedure for assessing MICs. In this study we determined MICs for blood culture isolates from @entity1 with @entity4963 by NCCLS M27-A and FACS methods and correlated the clinical outcome of these @entity1 with in vitro antifungal resistance test results. A total of 24 @entity1 with @entity4963 for whom one or more blood cultures were positive for a Candida sp. were included. Susceptibility testing was performed by NCCLS M27-A and FACS methods. The correlation of MICs (NCCLS M27-A and FACS) and clinical outcome was calculated. In 83% of the cases, the MICs of @entity2380 determined by FACS were within 1 dilution of the MICs determined by the NCCLS M27-A method. For proposed susceptibility breakpoints, there was 100% agreement between the M27-A and FACS methods. In the FACS assay, a @entity2380 MIC of <1 microg/ml was associated with cure (P < 0.001) whereas an MIC of > or =1 microg/ml was associated with @entity204 (P < 0.001). The @entity55658 -derived @entity2380 MICs did not correlate with outcome (P = 1 and P = 0.133).

Antifungal susceptibility testing of XXXX by flow cytometry correlates with clinical outcome.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity2937 :: ('942', 'Gene') :: ['FUN-1'] @entity2380 :: ('MESH:D015725', 'Chemical') :: ['fluconazole'] @entity4963 :: ('MESH:D058387', 'Disease') :: ['candidemia'] @entity55658 :: ('p|SUB|M|27|A', 'ProteinMutation') :: ['M27-A'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death']
@entity2380 :: (MESH:D015725,Chemical) :: ['fluconazole']
