Correct targeting of enzymes represents an important biological mechanism to control post-translational modifications of neurotransmitter receptors. The metabotropic @entity2227 receptor type 7 ( @entity10384 ) exists in two splice variants (mGluR7a and mGluR7b), defined by different C-termini that are phosphorylated by @entity7131 ( @entity7131 ). Recently, the search for @entity10384 a binding partners yielded several proteins that interacted with its C-terminus. Here, a yeast two-hybrid screen using the @entity10384 b C-terminus identified both variants of the catalytic gamma-subunit of protein phosphatase 1 (PP1gamma1 and PP1gamma2) as binding partners. The minimal interacting region of PP1gamma1/2 contained the core domain and was homologous to a region of @entity30152 that is needed for functional expression. Although this core domain is highly conserved within the protein phosphatase family, PP1alpha1 and @entity46997 did not interact with mGluR7b. Binding between PP1gamma1 and mGluR7b might be regulated by alternative splicing, as the variant-specific distal part of the @entity10384 b C-terminus mediated the interaction. Within this domain, @entity1522 involved in the binding to PP1gamma1 were mapped and biochemical assays using recombinant and native proteins verified the proposed interaction. Finally, the expression pattern of PP1gamma1, PP1gamma2 and mGluR7b was analysed in various CNS regions. In summary, these results suggest a regulation of mGluR7b by PP1gamma.

The metabotropic XXXX receptor mGluR7b binds to the catalytic gamma-subunit of protein phosphatase 1.

@entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity2227 :: ('CHEBI:29987', 'Chemical') :: ['glutamate'] @entity30152 :: ('5499', 'Gene') :: ['PP1alpha'] @entity10384 :: ('14807(Tax:10090)', 'Gene') :: ['mGluR7'] @entity7131 :: ('MESH:D020151', 'Disease') :: ['protein kinase C', 'PKC'] @entity46997 :: ('5500', 'Gene') :: ['PP1beta']
@entity2227 :: (CHEBI:29987,Chemical) :: ['glutamate']
