BACKGROUND: Post-transcriptional regulation by heterogeneous ribonucleoproteins (hnRNPs) is an important regulatory paradigm in @entity5 development. Our proteomic analysis revealed @entity24061 overexpression in @entity1697 as compared with normal mucosa; its role in @entity105 remains unknown. Here in we determined the @entity24061 associated protein networks and its clinical significance in @entity957 ( @entity957 ). METHODS: Immunoprecipitation (IP) followed by tandem mass spectrometry was used to identify the binding partners of @entity24061 in @entity5 cell lines. Ingenuity pathway analysis (IPA) was carried out to unravel the protein interaction networks associated with @entity24061 and key interactions were confirmed by co-IP-western blotting. @entity24061 expression was analyzed in 183 OSCCs, 44 @entity1697 and 106 normal tissues using immunohistochemistry (IHC) and correlated with clinico-pathological parameters and follow up data over a period of 91  months. Kaplan-Meier survival and Cox-multivariate-regression analyses were used to evaluate the prognostic significance of @entity24061 in @entity957 . RESULTS: We identified 345 binding partners of @entity24061 in @entity5 cells. IPA unraveled novel protein-protein interaction networks associated with @entity24061 and suggested its involvement in multiple cellular processes: DNA repair, replication, chromatin remodeling, cellular proliferation, RNA splicing and stability, thereby directing the fate of @entity5 cells. Protein-protein interactions of @entity24061 with 14-3-3  , @entity25931 and @entity7966 were confirmed using co-IP-western blotting. IHC analysis showed significant overexpression of nuclear @entity24061 in @entity1697 [p  =  0.001, Odds ratio (OR)  =  5.1, 95  % CI  =  2.1-11.1) and OSCCs (p  =  0.001, OR  =  8.1, 95  % CI  =  4.5-14.4) in comparison with normal mucosa. @entity957 @entity1 showing nuclear @entity24061 overexpression had significantly reduced recurrence free survival [p  =  0.026, Hazard ratio  =  1.95, 95  % CI  =  1.0-3.5] by Kaplan-Meier survival and Cox-multivariate-regression analyses and has potential to define a high-risk subgroup among @entity957 @entity1 with @entity66 . CONCLUSIONS: Our findings suggest novel functions of @entity24061 in cellular proliferation and survival, besides RNA splicing and stability in @entity5 . Association of nuclear @entity24061 with poor prognosis in @entity957 @entity1 taken together with its associated protein networks in @entity5 warrant future studies designed to explore its potential as a plausible novel target for molecular therapeutics.

Nuclear heterogeneous nuclear ribonucleoprotein D is associated with poor prognosis and interactome analysis reveals its novel binding partners in XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity25931 :: ('3190', 'Gene') :: ['hnRNPK'] @entity1697 :: ('MESH:D009062', 'Disease') :: ['oral dysplasia'] @entity66 :: ('MESH:D004194', 'Disease') :: ['nodal negative disease'] @entity7966 :: ('6280', 'Gene') :: ['S100A9'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer', 'oral cancer'] @entity957 :: ('MESH:D002294', 'Disease') :: ['OSCC', 'oral squamous cell carcinoma'] @entity24061 :: ('3184', 'Gene') :: ['hnRNPD'] @entity105 :: ('MESH:D063646', 'Disease') :: ['oral carcinogenesis']
@entity5 :: (MESH:D009369,Disease) :: ['oral cancer']
