The molecular mechanisms that link acute @entity788 (AP) and @entity881 remain unknown. To clarify the role of endothelial activation, we examined the effects of ascitic fluids from @entity35 with experimental @entity788 on the expression of adhesion molecules in @entity1 umbilical vein endothelial cells (HUVECs). Necrotizing @entity548 was induced with @entity7362 . Six and 24 h later, peritoneal exudates were collected, centrifuged and HUVECs were treated with the supernatants. The expression of @entity4105 , @entity2071 ( @entity2071 ) and @entity4725 ( @entity4725 ) was quantified by enzyme-linked immunosorbent assay. Induction of mRNA was assessed by reverse-transcriptase polymerase chain reaction. The activation of transcription factors was examined by electrophoretic mobility shift assay. The expression of @entity2071 in the tissues was examined immunohistochemically. @entity2071 and @entity4725 , but not @entity4105 expression was upregulated with comparable mRNA induction. Nuclear factor kappaB was activated, while @entity23601 binding activity was not altered. Immunohistochemically, enhanced @entity2071 expression was observed in the pancreas and lung, but not in the liver. Ascitic fluids may contain soluble factors responsible for the transcriptional activation of endothelial adhesion molecules, and @entity2071 may play roles in the pathogenesis of complicated AP.

Specific induction of adhesion molecules in XXXX vascular endothelial cells by @entity35 experimental @entity788 -associated ascitic fluids.

@entity1 :: ('9606', 'Species') :: ['human'] @entity788 :: ('MESH:D010190', 'Disease') :: ['pancreatitis'] @entity7362 :: ('MESH:D013656', 'Chemical') :: ['sodium taurocholate'] @entity548 :: ('MESH:D006470', 'Disease') :: ['hemorrhagic pancreatitis'] @entity4725 :: ('7412', 'Gene') :: ['vascular cell adhesion molecule-1', 'VCAM-1'] @entity23601 :: ('3727', 'Gene') :: ['activator protein-1'] @entity4105 :: ('6401', 'Gene') :: ['E-selectin'] @entity881 :: ('MESH:D009102', 'Disease') :: ['multiple organ failure'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity2071 :: ('3383', 'Gene') :: ['ICAM-1', 'intercellular adhesion molecule-1']
@entity1 :: (9606,Species) :: ['human']
