In most transmissible spongiform @entity75 prions accumulate in the @entity3329 ( @entity3329 ) long before they are detectable in the central nervous system. While a considerable body of evidence showed that B lymphocytes and follicular dendritic cells play a major role in prion colonization of lymphoid organs, the contribution of various other cell types, including antigen-presenting cells, to the accumulation and the spread of prions in the @entity3329 are not well understood. A comprehensive study to compare prion titers of candidate cell types has not been performed to date, mainly due to limitations in the scope of animal bioassays where prohibitively large numbers of @entity19 would be required to obtain sufficiently accurate data. By taking advantage of quantitative in vitro prion determination and magnetic-activated cell sorting, we studied the kinetics of prion accumulation in various splenic cell types at early stages of prion @entity281 . Robust estimates for infectious titers were obtained by statistical modelling using a generalized linear model. Whilst prions were detectable in B and T lymphocytes and in antigen-presenting cells like dendritic cells and macrophages, highest infectious titers were determined in two cell types that have previously not been associated with prion pathogenesis, plasmacytoid dendritic (pDC) and natural killer (NK) cells. At 30 days after @entity281 , NK cells were more than twice, and pDCs about seven-fold, as infectious as lymphocytes respectively. This result was unexpected since, in accordance to previous reports prion protein, an obligate requirement for prion replication, was undetectable in pDCs. This underscores the importance of prion sequestration and dissemination by antigen-presenting cells which are among the first cells of the immune system to encounter pathogens. We furthermore report the first evidence for a release of prions from lymphocytes and DCs of scrapie-infected @entity19 ex vivo, a process that is associated with a release of exosome-like membrane vesicles.

Plasmacytoid dendritic cells sequester high prion titres at early stages of prion XXXX .

@entity75 :: ('MESH:D001927', 'Disease') :: ['encephalopathies'] @entity3329 :: ('MESH:D008206', 'Disease') :: ['lymphoreticular system', 'LRS'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection']
@entity281 :: (MESH:D007239,Disease) :: ['infection']
