BACKGROUND: Cytostatic isolated lung perfusion has been advocated for treating @entity358 of soft tissue @entity242 . Different techniques of isolated lung perfusion have been developed. METHODS: Isolated lung perfusion with and without @entity632 was performed on white @entity1707 during 15 minutes either by a single-pass system (n = 7) or by a recirculating-blood perfusion system (n = 7). Three animals with endovenous drug application served as controls. Leakage was assessed using isotopic tracers. Perfusion-induced lung tissue injury was determined by postperfusion chest radiographs, by angiotensin-converting enzyme-to-protein ratio in the plasma and in the bronchioalveolar lavage fluid, and by wet-to-dry weight ratio and histologic examination of lung biopsy specimens at 20 and 50 minutes. @entity632 concentration in lung tissue and plasma was compared between the three study groups. RESULTS: All isolated lung perfusion studies were successfully performed without significant @entity262 (< 0.6%). Wet-to-dry weight ratio was significantly lower after single-pass as compared with recirculating-blood perfusion and endovenous drug application at both time points (5.0 +/- 1.1 and 5.3 +/- 0.8 for single-pass versus 6.6 +/- 1.1 and 6.9 +/- 0.5 for recirculating-blood versus 6.6 +/- 0.2 and 5.9 +/- 0.7 for the control group, respectively; p < 0.05). Angiotensin-converting enzyme-to-protein plasma ratio in the single-pass group was significantly lower only at 20 minutes (6.3 +/- 2.4 versus 9.3 +/- 1.0 versus 9.7 +/- 1.9, respectively; p < 0.05) but not at 50 minutes. Angiotensin-converting enzyme-to-protein ratio in bronchoalveolar lavage fluid, histology of lung biopsy specimens, and chest radiographs did not differ significantly between the three groups. @entity632 lung tissue concentration was not significantly different after single-pass (17.5 micrograms/g) and recirculating-blood perfusion (21.9 micrograms/g), but was significantly higher than after endovenous drug application (3.0 micrograms/g; p < 0.01). CONCLUSIONS: Both isolated lung perfusion techniques resulted in a sixfold to sevenfold higher @entity632 lung tissue concentration than after endovenous application. Isolated lung perfusion-induced @entity651 was similar for both techniques, but recirculating-blood perfusion appeared to result in more @entity647 and was technically more demanding than single-pass perfusion.

Isolated lung perfusion: single-pass system versus recirculating blood perfusion in XXXX .

@entity632 :: ('MESH:D004317', 'Chemical') :: ['doxorubicin', 'Doxorubicin'] @entity1707 :: ('9823', 'Species') :: ['pigs'] @entity242 :: ('MESH:D012509', 'Disease') :: ['sarcoma'] @entity262 :: ('MESH:D034721', 'Disease') :: ['systemic leakage'] @entity651 :: ('MESH:D055370', 'Disease') :: ['lung injury'] @entity358 :: ('MESH:D008171', 'Disease') :: ['pulmonary metastasis'] @entity647 :: ('MESH:D055371', 'Disease') :: ['acute lung injury']
@entity1707 :: (9823,Species) :: ['pigs']
