ETHNOPHARMACOLOGICAL RELEVANCE: Pyrostegia venusta is a native Brazilian plant which has a variety of uses in traditional folk medicine including the treatment of @entity1694 . However, its effectiveness on melanogenesis is not yet elucidated. AIM OF THE STUDY: This study aimed to investigate the melanogenic activity of hydroalcoholic extracts from the leaves and flowers of @entity69858 on @entity19 @entity19 @entity320 cells. MATERIALS AND METHODS: Different concentrations of the hydroalcoholic extracts of flowers and leaves of @entity69858 were evaluated in trials of spontaneous melanin content (4 days), and cell viability by the @entity98 assay in @entity19 @entity19 cells, and in the mushroom tyrosinase activity in vitro. RESULTS: Both extracts, leaves (0.1; 0.3; 1 and 3  g/mL) and flowers (0.03 and 0.1  g/mL) increased the melanin content in a concentration dependent manner after 4 days of incubation on @entity320 cells. Leaves extract promoted enhancement of melanogenesis with maximum effect of 33.3  3% (3  g/mL), and the flower extract increased in 23.4  3% (0.1  g/mL). The cell viability test using @entity98 showed that in the same tested concentrations of both extracts no cell death was detected. Actually, either extract was not able to cause any change in the tyrosinase activity. HPLC analysis of @entity69858 extracts found 0.09% and 1.08% of @entity13579 on leaves and flowers extracts, respectively. CONCLUSIONS: The leaves and flowers extracts of @entity69858 stimulates B16F10 melanogenesis at very low concentrations. These findings support the folk medicinal use of @entity69858 on the treatment of @entity4182 , such as @entity1694 .

Hyperpigmentant activity of leaves and flowers extracts of Pyrostegia venusta on @entity19 B16F10 XXXX .

@entity4182 :: ('MESH:D017496', 'Disease') :: ['hypopigmentation diseases'] @entity19 :: ('10090', 'Species') :: ['B16F10', 'murine'] @entity1694 :: ('MESH:D014820', 'Disease') :: ['vitiligo'] @entity13579 :: ('MESH:D000481', 'Chemical') :: ['allantoin'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity69858 :: ('38123', 'Species') :: ['P. venusta'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma']
@entity320 :: (MESH:D008545,Disease) :: ['melanoma']
