@entity117 ( @entity1281 ) and other nonsteroidal anti-inflammatory drugs (NSAIDs) inhibit colorectal tumorigenesis. Apoptosis is a critical determinant of tissue mass homeostasis and may play a role in @entity105 . We studied the effect of @entity1281 on the survival of a @entity1 @entity694 cell line using more sensitive methods than we had applied previously. @entity1281 induced apoptosis in HT-29 @entity694 cells at concentrations > or =1 mM as established by: (a) morphological changes consistent with apoptosis in cells examined by fluorescence microscopy and semi-thin cell sections, and (b) DNA strand breaks: 45% of the cells were TdT-mediated @entity1977 nick end labeling (TUNEL) positive at 3 mM at 72 hr, and 70% were positive by the comet assay. Electron microscopy also confirmed the induction of apoptosis by @entity1281 . @entity1281 -induced apoptosis was not associated with: (a) a ladder pattern on genomic DNA electrophoresis, or (b) a subdiploid peak on flow cytometry. Apoptotic bodies were virtually absent on standard morphological assessments and only a few were detected on semi-thin sections. For the above reasons, this apoptosis induced by @entity1281 is "atypical," and the unusual features of @entity1281 -induced apoptosis, besides their taxonomic value, may offer clues to the mechanisms that control the process of apoptosis or perhaps the @entity5 chemopreventive properties of this compound. These findings demonstrate that @entity1281 induces apoptosis in @entity1 @entity694 cells, bolstering the hypothesis that apoptosis may be a mechanism by which NSAIDs inhibit @entity694 . These findings should be examined in animal and/or clinical research studies in vivo.

Effect of XXXX on induction of apoptosis in HT-29 @entity1 @entity694 cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity117 :: ('MESH:D001241', 'Chemical') :: ['Aspirin'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon carcinogenesis', 'colon adenocarcinoma', 'colon cancer'] @entity1977 :: ('CHEBI:17625', 'Chemical') :: ['dUTP'] @entity105 :: ('MESH:D063646', 'Disease') :: ['carcinogenesis'] @entity1281 :: ('CHEBI:15365', 'Chemical') :: ['ASA']
@entity117 :: (MESH:D001241,Chemical) :: ['aspirin']
