PURPOSE: We aimed to investigate the methylation pattern in @entity150 and assess the diagnostic potential of such epigenetic changes in urine. EXPERIMENTAL DESIGN: The methylation status of 7 genes ( @entity4882 , @entity2529 , @entity5962 , @entity986 , @entity9288 , @entity4191 , and @entity8724 ) in 98 cases of @entity150 and 4 cases of @entity2735 was analyzed by methylation-specific PCR. Twenty-two cases had paired voided urine samples for analysis. RESULTS: In transitional cell @entity5 tissues, aberrant methylation was frequently detected in @entity4882 (87.8%), @entity2529 (58.2%), @entity5962 (63.3%), and @entity986 (26.5%), whereas methylation of @entity9288 (13.3%), @entity4191 (5.1%), and @entity8724 (5.1%) is not common. No association between methylation status and grading or muscle invasiveness was demonstrated. In 22 paired voided urine samples of @entity150 , methylation of @entity2529 , @entity4882 , @entity5962 , and @entity986 could be detected in 45.5%, 68.2%, 59.1%, and 13.6% of the cases, respectively. The sensitivity of methylation analysis (90.9%) was higher than that of urine cytology (45.5%) for @entity5 detection. Methylation of @entity4882 (50%), @entity2529 (75%), and @entity5962 (50%) was also detected in @entity2735 . In 7 normal urothelium samples and 17 normal urine controls, no aberrant methylation was detected except for @entity4882 methylation in 3 normal urothelium samples (42.9%) and 4 normal urine samples (23.5%), respectively. CONCLUSIONS: Our results demonstrated a distinct methylation pattern in @entity150 with frequent methylation of @entity4882 , @entity2529 , @entity5962 , and @entity986 . Detection of gene methylation in routine voided urine using selected markers appeared to be more sensitive than conventional urine cytology.

Hypermethylation of multiple genes in @entity5 tissues and voided urine in XXXX patients.

@entity2529 :: ('1612', 'Gene') :: ['DAPK'] @entity2735 :: ('MESH:D002278', 'Disease') :: ['carcinoma in situ'] @entity986 :: ('1029', 'Gene') :: ['p16'] @entity5 :: ('MESH:D009369', 'Disease') :: ['carcinoma tumor', 'cancer'] @entity9288 :: ('1030', 'Gene') :: ['p15'] @entity5962 :: ('999', 'Gene') :: ['E-cadherin'] @entity8724 :: ('4255', 'Gene') :: ['MGMT'] @entity150 :: ('MESH:D001749', 'Disease') :: ['bladder transitional cell carcinoma', 'bladder cancer'] @entity4191 :: ('2950', 'Gene') :: ['GSTP1'] @entity4882 :: ('5915', 'Gene') :: ['RARbeta']
@entity150 :: (MESH:D001749,Disease) :: ['urinary bladder cancer']
