The membrane-permeable, noncompetitive @entity671 ( @entity9423 inhibitor @entity38497 ( @entity15258 ) has been widely used to probe for @entity9423 involvement in @entity460 signaling pathways. However, a number of recent studies in different cell types revealed other sites of action of @entity15258 . In this study, we examined the influence of @entity15258 on capacitative @entity460 entry and intracellular @entity141 concentrations in @entity35 @entity32651 ( @entity35 m1) cells. @entity15258 was found to inhibit capacitative @entity460 entry, but at concentrations greater than 50 microM, a new effect of @entity15258 was observed. When capacitative @entity460 entry was blocked with @entity15311 , @entity15258 caused an increase in cytoplasmic @entity141 . This increase in intracellular @entity141 was not caused by altered buffering of cytoplasmic @entity141 and was not caused by or in any way affected by the depletion of intracellular @entity141 stores. Associated with the increase in intracellular @entity141 , in the presence of 2 mM @entity141 , @entity15258 activated single channels in the plasma membrane with a conductance of approximately 50 pS. These channels seem to be nonselective cation channels; monovalent cations are the major carriers of current, but finite permeability to @entity141 leads to a significant intracellular @entity141 signal. Experiments with excised patches indicate that @entity15258 activates these channels from the outer aspect of the cell membrane. This effect of @entity15258 further illustrates the complex actions of this compound and reveals the presence in @entity35 m1 cells of a novel, ligand-gated @entity460 -permeable channel.

@entity38497 activates a novel XXXX -permeable cation channel.

@entity671 :: ('MESH:D015544', 'Chemical') :: ['inositol 1,4,5-trisphosphate'] @entity35 :: ('10116', 'Species') :: ['rat', 'RBL-2H3'] @entity9423 :: ('25679(Tax:10116)', 'Gene') :: ['IP3-receptor', 'IP3)-receptor'] @entity15311 :: ('MESH:C026226', 'Chemical') :: ['Gd3+'] @entity15258 :: ('MESH:C109986', 'Chemical') :: ['2-APB'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity32651 :: ('MESH:D015471', 'Disease') :: ['basophilic leukemia'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+'] @entity38497 :: ('MESH:C504876', 'Chemical') :: ['2-aminoethoxydiphenyl borane']
@entity460 :: (MESH:D002118,Chemical) :: ['calcium']
