Nuclear transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotency but cause no @entity1200 . Although ntESCs are able to differentiate into somatic cells, the ability of ntESCs to differentiate into primordial germ cells (PGCs) has not been examined. In this work, we examined the capacity of @entity19 ntESCs to differentiate into PGCs in vitro. ntESCs aggregated to form embryoid bodies (EB) in EB culture medium supplemented with @entity7739 ( @entity7739 ) as the differentiation factor. The expression level of specific PGC genes was compared at days 4 and 8 using real time PCR. Flow cytometry and immunocytochemical staining were used to detect @entity29921 as a specific PGC marker. ntESCs expressed particular genes related to different stages of PGC development. Flow cytometry and immunocytochemical staining confirmed the presence of @entity29921 protein in a small number of cells. There were significant differences between cells that differentiated into PGCs in the group treated with @entity7739 compared to non-treated cells. These findings indicate that ntESCs can differentiate into putative PGCs. Improvement of ntESC differentiation into PGCs may be a reliable means of producing mature germ cells.

The ability of XXXX nuclear transfer embryonic stem cells to differentiate into primordial germ cells.

@entity19 :: ('10090', 'Species') :: ['mouse'] @entity29921 :: ('13206(Tax:10090)', 'Gene') :: ['Mvh'] @entity1200 :: ('MESH:D007154', 'Disease') :: ['immunological disorders'] @entity7739 :: ('12159(Tax:10090)', 'Gene') :: ['BMP4', 'bone morphogenetic protein 4', 'Bmp4']
@entity19 :: (10090,Species) :: ['mouse']
