A regulatory role for @entity7131 ( @entity7131 ) and @entity1813 has been implicated in the control of @entity0 cell growth and function. Here we report on the effects of the two @entity7131 agonists @entity864 ( @entity865 ) and @entity10186 on @entity1426 metabolism, @entity1421 ( @entity1421 ) production, and growth in MDA MB 231 @entity1 @entity0 cells. @entity865 caused a dose-dependent increase in @entity1421 production which was maximal at 100 nM and which was blocked in the presence of an equimolar concentration of @entity10186 . @entity10186 alone had no effect on @entity1421 synthesis. Both @entity865 and @entity10186 stimulated @entity1426 release and reduced @entity844 incorporation into @entity4357 , their combined effect being less than additive in co-incubation. @entity1356 ( @entity1356 ) induced a tenfold and twofold synergistic increase in @entity1421 production in the presence of @entity865 (10 nM) and @entity10186 (10 nM) respectively. @entity10186 caused a dose-dependent inhibition of the @entity4621 -potentiated @entity1356 response. Treatment of MDA MB 231 cells for 4 days with @entity865 (10 nM) or @entity10186 (10 nM) inhibited cell growth by 74% and 20% respectively. Co-treatment with both @entity7131 agonists reversed the anti-proliferative effect of @entity865 to that seen with @entity10186 alone. In contrast the anti-proliferative action of @entity2367 , another @entity7131 modulator, was unaffected in the presence of @entity10186 . @entity865 also induced morphological changes in MDA MB 231 cells which were prevented by co-treatment with @entity10186 . This study further supports a regulatory role for @entity7131 in the control of eicosanoid synthesis and growth in @entity1 @entity0 cells. Although the findings are consistent with @entity10186 acting as an antagonist/weak agonist in relation to @entity865 action, the mechanistic basis for this differential action of @entity865 and @entity10186 is uncertain.

Differential effects of @entity7131 agonists on prostaglandin production and growth in @entity1 XXXX cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity844 :: ('CHEBI:35366', 'Chemical') :: ['fatty acid'] @entity10186 :: ('MESH:C046785', 'Chemical') :: ['Bryostatin 1', 'bryostatin 1'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity1356 :: ('3553', 'Gene') :: ['Interleukin-1beta', 'IL-1beta'] @entity1421 :: ('MESH:D015232', 'Chemical') :: ['PGE2', 'prostaglandin E2'] @entity1426 :: ('MESH:D016718', 'Chemical') :: ['arachidonic acid'] @entity7131 :: ('MESH:D020151', 'Disease') :: ['protein kinase C', 'PKC'] @entity4621 :: ('CHEBI:37532', 'Chemical') :: ['phorbol ester'] @entity2367 :: ('CHEBI:17761', 'Chemical') :: ['ceramide'] @entity865 :: ('CHEBI:61432', 'Chemical') :: ['TPA'] @entity1813 :: ('MESH:D015777', 'Chemical') :: ['eicosanoids'] @entity864 :: ('MESH:C554292|MESH:D013755', 'Chemical') :: ['12-O-tetradecanoyl-phorbol-13-acetate'] @entity4357 :: ('MESH:D010716', 'Chemical') :: ['phosphatidylinositol']
@entity0 :: (MESH:D001943,Disease) :: ['breast cancer']
