OBJECTIVE: To evaluate the relationship between inhibition of proteoglycans which secreted by salivary @entity2347 cell line (SACC-83) and the neurotropic ability of the @entity5 cells. METHODS: The expression vector of short hairpin RNA (shRNA-WJ4) targeting @entity70076 gene was constructed and transfected into SACC-83 cells (group SACC83-WJ4), shRNA-HK used as negative control was transfected into SACC-83 cells (group SACC-83-HK), SACC-83 cells without transfection was used as black control (group SACC-83). The @entity70076 gene expression was measured by real-time PCR. The content of proteoglycans was detected by Blyscan Assay Kit. The effect of down-regulated proteoglycans on the perineural invasion of @entity19 was observed. All data were analyzed by the software spss 13.0. RESULTS: The results showed that the transfected efficiency of shRNA was 43.3%. The expression of @entity70076 was inhibited by 43.0% 48 h after transfection of shRNA-WJ4. The content of proteoglycans was down-regulated by 30.25% 48 h after transfection. In the neurotropic experiment in vivo of @entity19 , the rate of perineural invasion of group SACC-83-WJ4 was 33.33%, significantly lower than that of the negative control (100%) and the black control (100%) (P < 0.05). CONCLUSION: @entity70076 gene of SACC cells was silenced by RNA interference technology, proteoglycans secretion was reduced and neurotropic invasion behavior of SACC was inhibited obviously.

The effect of proteoglycans inhibited on the neurotropic growth of salivary XXXX .

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity2347 :: ('MESH:D003528', 'Disease') :: ['adenoid cystic carcinoma'] @entity19 :: ('10090', 'Species') :: ['nude mice'] @entity70076 :: ('233781(Tax:10090)', 'Gene') :: ['xylosyltransferase-I', 'Xylosyltransferase-I']
@entity2347 :: (MESH:D003528,Disease) :: ['adenoid cystic carcinoma']
