UNASSIGNED: The umbilical cord (UC) represents an important source of mesenchymal stem cells ( @entity16591 ). These @entity1 UC-derived @entity16591 (UC- @entity16591 ) have already been isolated using a protocol based on the migratory and plastic adhesive properties of @entity16591 ( @entity16591 ). The @entity16591 isolation method, however, is difficult to standardize. Therefore, we developed an enzymatic isolation protocol (UC- @entity16591 -Enz) to overcome the above mentioned disadvantages. First, we investigated the UC- @entity16591 -Enz for their @entity16591 properties. We found that UC- @entity16591 -Enz express the @entity16591 markers @entity12359 , @entity5399 and CD105 and are able to differentiate into osteoblasts, adipocytes and chondroblasts fulfilling the @entity16591 criteria of the International Society for Cellular Therapy. Previously we found that @entity16591 are unique among MSCs due to their significant expression of several hepatic (progenitor) markers. Therefore, we also investigated the expression of hepatic transcription factors and other hepatic markers in UC- @entity16591 -Enz at both the mRNA and protein level. We found that the expression of hepatic transcription factors ( @entity15774 , @entity43289 , @entity1935 and @entity13050 ) and hepatic markers ( @entity160 , @entity5735 , @entity495 , @entity5741 , @entity8259 , @entity2873 and @entity4166 ) in UC- @entity16591 -Enz was not significantly different from those of @entity16591 . Consequently, this optimized enzyme-based method represents a fast, robust and standardized way to isolate UC- @entity16591 for a broad range of applications.

Evaluation of a new standardized enzymatic isolation protocol for XXXX umbilical cord-derived stem cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity43289 :: ('2627', 'Gene') :: ['GATA6'] @entity4166 :: ('3880', 'Gene') :: ['KRT19'] @entity16591 :: ('9242', 'Gene') :: ['MSC', 'UC-MSC-Mig'] @entity5735 :: ('1803', 'Gene') :: ['DPP4'] @entity495 :: ('2697', 'Gene') :: ['CX43'] @entity13050 :: ('64321', 'Gene') :: ['SOX17'] @entity8259 :: ('1829', 'Gene') :: ['DSG2'] @entity5399 :: ('7070', 'Gene') :: ['CD90'] @entity5741 :: ('22943', 'Gene') :: ['DKK1'] @entity160 :: ('174', 'Gene') :: ['AFP'] @entity2873 :: ('3875', 'Gene') :: ['KRT18'] @entity12359 :: ('4907', 'Gene') :: ['CD73'] @entity15774 :: ('2626', 'Gene') :: ['GATA4'] @entity1935 :: ('6662', 'Gene') :: ['SOX9']
@entity1 :: (9606,Species) :: ['human']
