BACKGROUND AIMS: Adipose-derived stem cells (ASCs) represent an important source of cell therapy to treat @entity6 complications. However, @entity4854 may alter several cellular functions, so the present study aimed to investigate the influence of a @entity6 environment on the @entity3308 and differentiation capabilities of ASCs. METHODS: @entity1 ASCs were obtained from subcutaneous adipose tissues of @entity6 (dASCs) and nondiabetic donors (nASCs) and characterized. To reproduce an in vitro @entity4854 environment, the nASCs were also cultured under prolonged high- @entity413 (HG; 4.5   g/L) or low- @entity413 (LG; 1.0   g/L) conditions. RESULTS: The expression of cell surface markers in dASCs and nASC was similar and characteristic of mesenchymal stem cells. Although dASCs or HG-treated nASCs exhibited decreased proliferation, enhanced expression of the pluripotent markers @entity4731 , @entity4729 , and Nanog was observed. Moreover, HG-treated nASCs exhibited decreased cell migration, enhanced senescence, and significantly higher intracellular reactive @entity26 species (ROS), whereas their adipogenic and osteogenic differentiation capacities remained comparable to LG-treated cells. With antioxidant treatment, HG-treated nASCs showed improved cell proliferative activity without @entity3308 enhancement. This HG-induced biological response was associated with ROS-mediated AKT attenuation. When cultured in an appropriate induction medium, the HG-treated nASCs and dASCs exhibited enhanced potential of transdifferentiation into neuron-like cells. DISCUSSION: Despite lower proliferative activity and higher senescence in a @entity6 environment, ASCs also exhibit enhanced @entity3308 and neurogenic transdifferentiation potential via a ROS-mediated mechanism. The information is important for future application of autologous ASCs in @entity6 @entity1 .

High @entity413 -induced reactive @entity26 species generation promotes XXXX in @entity1 adipose-derived stem cells.

@entity1 :: ('9606', 'Species') :: ['patients', 'Human'] @entity3308 :: ('MESH:D020295', 'Disease') :: ['stemness'] @entity4729 :: ('5460', 'Gene') :: ['Oct-4'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetic'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity4731 :: ('6657', 'Gene') :: ['Sox-2'] @entity4854 :: ('MESH:D006943', 'Disease') :: ['hyperglycemia'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity3308 :: (MESH:D020295,Disease) :: ['stemness']
