The participation of different @entity460 -regulated mechanisms in the generation of cytosolic @entity141 transients during neuronal excitation has been compared in isolated large and small primary (dorsal root ganglia (DRG)) and secondary (spinal @entity11989 ( @entity11989 )) @entity35 sensory neurones. As it was shown before in @entity19 primary sensory neurones the application of mitochondrial protonophore CCCP by itself induced only small elevation of [ @entity141 ](i). However, its preceding application substantially increased the peak amplitude of depolarization-induced transients. Application of CCCP immediately after termination of the depolarizing pulse induced in both types of primary neurones a massive release of @entity141 from mitochondria into the cytosol. In secondary neurones application of @entity6320 by itself induced a substantial release of @entity141 from the mitochondria, but its preceding application resulted in only an insignificant increase in the peak amplitude of depolarization-triggered @entity460 transients. Application of CCCP immediately after termination of depolarization elicited a small release of @entity141 , which became more pronounced when the application was delayed. Preceding application of CCCP increased the amplitude of the transients induced by @entity5834 -triggered @entity141 release from the endoplasmic reticulum in secondary neurones and did not affect those in large primary neurones. These findings may be explained by substantial differences in the density and distribution of mitochondria in the cytosol of primary and secondary sensory neurones. This suggestion was confirmed electronmicroscopically, showing a much lower density of mitochondria near plasmalemma in secondary sensory neurones and predominant clustered location of mitochondria beneath the plasmalemma in the primary cells. The possible functional importance of these differences is discussed.

Role of mitochondria in intracellular XXXX signaling in primary and secondary sensory neurones of @entity35 .

@entity6320 :: ('MESH:D002258', 'Chemical') :: ['CCCP'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity5834 :: ('MESH:D002110', 'Chemical') :: ['caffeine'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca(2+)'] @entity11989 :: ('MESH:D009261', 'Disease') :: ['DH', 'dorsal horn']
@entity460 :: (MESH:D002118,Chemical) :: ['calcium']
