A family of proteins designated BSP-A1, BSP-A2, @entity70381 and BSP-30 kDa (collectively called BSP proteins for @entity291 Seminal Plasma proteins) constitute the major protein fraction in the bull seminal plasma. These proteins interact with @entity1877 phospholipids on the sperm surface and play a role in the membrane stabilization (decapacitation) and destabilization (capacitation) process. Homologous proteins have been isolated from boar and stallion seminal plasma. In the current study we report the isolation and preliminary characterization of homologous proteins from @entity1237 seminal plasma. Frozen semen (-80 degrees C) was thawed and centrifuged to remove sperm. The proteins in the supernatant were precipitated by the addition of cold @entity1068 . The precipitates were dissolved in @entity43789 and lyophilised. The lyophilised proteins were dissolved in @entity1391 buffer and loaded onto a gelatin-agarose column, which was previously equilibrated with the same buffer. The column was successively washed with @entity1391 buffer, with @entity1391 buffer saline and with 0.5 M @entity1133 in @entity1391 buffer saline to remove unadsorbed proteins, and the adsorbed proteins were eluted with 5 M @entity1133 in @entity1391 buffer saline. Analysis of pooled, dialysed and lyophilised gelatin-agarose adsorbed protein fraction by @entity214 -PAGE indicated the presence of four protein bands that were designated GSP-14 kDa, GSP-15 kDa, GSP-20 kDa and GSP-22 kDa (GSP, @entity1237 Seminal Plasma proteins). Heparin-affinity chromatography was then used for the separation of GSP-20 and -22 kDa from GSP-14 and -15 kDa. Finally, HPLC separation permitted further isolation of each one from the other. @entity535 sequence analysis of these proteins indicated that they are homologous to BSP proteins. In addition, these BSP homologs bind to hen's egg-yolk low-density lipoproteins. These results together with our previous data indicate that BSP family proteins are ubiquitous in @entity1 seminal plasma, exist in several forms in each species and possibly play a common biological role.

Isolation and characterization of gelatin-binding proteins from XXXX seminal plasma.

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity70381 :: ('317695(Tax:9913)', 'Gene') :: ['BSP-A3'] @entity291 :: ('9913', 'Species') :: ['Bovine'] @entity1133 :: ('MESH:D014508', 'Chemical') :: ['urea'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['Amino acid'] @entity43789 :: ('MESH:C027043', 'Chemical') :: ['ammonium bicarbonate'] @entity1877 :: ('MESH:D002794', 'Chemical') :: ['choline'] @entity1391 :: ('CHEBI:26020', 'Chemical') :: ['phosphate'] @entity1068 :: ('MESH:D000431', 'Chemical') :: ['ethanol'] @entity1237 :: ('9925', 'Species') :: ['Goat', 'goat'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS']
@entity1237 :: (9925,Species) :: ['goat']
