INTRODUCTION:: Preeclampsia affects 5-7% of all healthy pregnancies and is characterized by @entity101 and @entity644 . Although the pathogenesis of @entity1468 is still not fully understood, a failure of spiral artery transformation and aberrant placental vasculature are considered to be facets of this disease. Studies have also implicated increased autophagic activity. In this study, we investigated whether oxidative stress could increase autophagic activity and consequently affect trophoblast invasion and the placental vasculature. METHODS:: Placentas from 18 pregnancies complicated by @entity1468 and from 18 uncomplicated pregnancies, trophoblast HTR8/SVneo cell line (HTR8/SVneo) extravillous trophoblasts, and @entity1 umbilical vein endothelial cells (HUVECs) were employed. The levels of autophagy markers @entity21684 , @entity8783 and autophagosome were quantified by immunohistochemistry, Western blotting and RT-PCR in placental tissue, and in trophoblasts and endothelial cells that had been treated with an oxidative stress inducer @entity413 oxidase. Trophoblast invasion and endothelial cell tube formation were assessed in HTR8/SVneo cells or HUVECs that had been treated with @entity413 oxidase. RESULTS:: The expression of @entity21684 , @entity8783 and autophagosome was significantly increased in placentas from pregnancies complicated by early-onset @entity1468 and in HTR8/SVneo cells and HUVECs treated with @entity413 oxidase. In addition, trophoblast invasion and endothelial cell tube formation were significantly reduced in HTR8/SVneo cells or HUVECs that had been treated with @entity413 oxidase. CONCLUSION:: Our data suggest that oxidative stress induces increased autophagy in trophoblasts or endothelial cells which affects trophoblast invasion and the placental vasculature. Excessive autophagic activity may be involved in the development of @entity1468 .

Excessive autophagy induces the failure of trophoblast invasion and vasculature: possible relevance to the pathogenesis of XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity644 :: ('MESH:D011507', 'Disease') :: ['proteinuria'] @entity101 :: ('MESH:D006973', 'Disease') :: ['hypertension'] @entity8783 :: ('8678', 'Gene') :: ['Beclin-1'] @entity1468 :: ('MESH:D011225', 'Disease') :: ['preeclampsia'] @entity21684 :: ('84557', 'Gene') :: ['LC3'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity1468 :: (MESH:D011225,Disease) :: ['preeclampsia']
