UNASSIGNED: The ocular microcirculation represents an important target to treat @entity2194 , where @entity1503 flow plays key role as, for example, in anterior @entity1596 . To evaluate novel interventions targeting the microcirculation, appropriate and reliable tools to study this particular microvascular bed are needed. Intravital microscopy (IVM) belongs to several methods allowing evaluation of microcirculation experimentally, even in small animals. The aim of our study was to examine the iridial microcirculation (IMIC) in @entity1596 induced by local or systemic endotoxin administration in @entity35 and @entity19 by IVM and to propose new parameters to quantify the changes within the IMIC. Systemic @entity32 was induced in @entity35 by intravenous endotoxin administration, control group received normal saline intravenously. Local @entity32 was induced in @entity19 by intravitreal endotoxin administration, the control group received normal saline intravitreally. IVM of IMIC was performed in animals receiving systemic endotoxin prior injection and 1 and 2 h afterwards, respectively, in animals receiving intravitreal endotoxin/saline prior local injection and 5 h afterwards. Obtained video recordings were analyzed off-line. @entity177 ( @entity177 ) and dysfunctional capillary density (DCD) were evaluated for description of IMIC, and calculation of @entity177 /DCD ratio was performed. In systemic @entity32 , @entity177 was significantly decreased compared to control animals. In @entity32 , the number of functional capillaries in the IMIC was significantly reduced following the endotoxin challenge. Analysis of the DCD revealed a significant increase in capillaries with reduced perfusion after intravitreal endotoxin administration and right shift of the @entity177 /DCD ratio was observed after endotoxin local injection. Detecting and quantifying changes in IMIC during systemic or @entity32 in experimental animals by IVM was feasible. Therefore, IVM of the IMIC represents a valuable tool to evaluate and quantify inflammatory changes in experimental @entity2194 .

Evaluation of iris functional capillary density in experimental local and systemic XXXX .

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation', 'local inflammation'] @entity2194 :: ('MESH:D005128', 'Disease') :: ['inflammatory diseases of eye', 'eye disease'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity1596 :: ('MESH:D014605', 'Disease') :: ['uveitis'] @entity1503 :: ('MESH:D006402', 'Disease') :: ['impairment of microvascular blood'] @entity177 :: ('MESH:D051437', 'Disease') :: ['FCD', 'Functional capillary density'] @entity35 :: ('10116', 'Species') :: ['rats']
@entity32 :: (MESH:D007249,Disease) :: ['inflammation']
