1. Effects of dietary conjugated @entity4722 (CLA) on various functional properties of macrophages and heterophils in the laying hen were determined. 2. Seventy two 28-week-old-ISA brown hens were randomly assigned to one of two experimental groups and were fed diets containing 0 or 1% CLA for a total period of 18 weeks. Blood samples were collected from 6 hens per diet group every 3 weeks and macrophages and heterophils were isolated. The following variables were determined in resting and @entity4514 -activated (65 microM) macrophages and heterophils: total cell-associated urokinase plasminogen activator ( @entity2290 ), membrane-bound @entity2290 , free @entity2290 binding sites and @entity224 production. 3. There were no significant differences between diet groups throughout the whole experimental period in total cell-associated @entity2290 , membrane-bound @entity2290 , free @entity2290 binding sites and @entity224 production by resting macrophages and heterophils. 4. Activated heterophils and macrophages isolated from hens fed the CLA-supplemented diet had higher membrane-bound @entity2290 activity compared with the corresponding values for activated heterophils and macrophages obtained from hens fed the basal diet. These differences were significant for heterophils during weeks 6, 12, 15 and 18 and for macrophages during weeks 6, 9 and 12 of the experiment. 5. Macrophages and heterophils from hens fed the CLA diet had lower @entity224 production compared with the corresponding cells from the control hens on weeks 12, 15 and 18 of the experiment. 6. Dietary CLA modulated certain aspects of the immune system in the laying hen. The increased quantity of @entity2290 on the membrane of macrophages and heterophils isolated from hens fed the CLA diet may facilitate the ability of these cells to reach the point of a potential @entity32 (pro-inflammatory effect). 7. In contrast to the effect on the @entity2290 system, dietary CLA reduced @entity224 production by activated macrophages and heterophils during the second half (last 9 weeks) of the experimental period suggesting that CLA may exert an anti-inflammatory effect in the laying hen.

Effects of dietary conjugated XXXX isomers on several functional properties of macrophages and heterophils in laying hens.

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity4722 :: ('MESH:D019787', 'Chemical') :: ['linoleic acid'] @entity4514 :: ('MESH:D013755', 'Chemical') :: ['phorbol myristate acetate'] @entity224 :: ('MESH:D013481', 'Chemical') :: ['superoxide'] @entity2290 :: ('5328', 'Gene') :: ['u-PA']
@entity4722 :: (MESH:D019787,Chemical) :: ['linoleic acid']
