UNASSIGNED: In a lentivirus-based gene delivery system, the incorporated gene is continuously expressed for a long time. In this study, we devised a simple way to knock down a specific gene in a kidney cell-specific pattern in adult @entity19 by lentivirus-assisted transfer of short hairpin RNA (shRNA). Kidney @entity1358 ( @entity1358 )-specific aquaporin-3 ( @entity23071 )-knockdown @entity19 were generated by consecutive injection of @entity10750 -Cre-expressing lentivirus (LV- @entity10750 Cre) and loxP- @entity23071 shRNA-expressing lentivirus (LV-loxP shAQP3) in adult C57BL6/J @entity19 . LV- @entity10750 Cre was designed to express mCherry, while LV-loxP shAQP3 was designed with a floxed enhanced green fluorescent protein (EGFP)-tagged stop sequence, and thus EGFP would be expressed only in the absence of Cre recombination. In @entity19 treated with LV- @entity10750 Cre alone, mCherry protein expression, which indicates the presence of Cre recombinase, occurred only in @entity1358 cells. However, LV-loxP shAQP3 injection alone resulted in an increase in EGFP expression in all kidney cells, indicating the transcription of the floxed region. When LV- @entity10750 Cre and LV-loxP shAQP3 were sequentially transduced, EGFP expression was attenuated while mCherry expression was sustained in @entity1358 cells, demonstrating a @entity1358 cell-specific recombination of the floxed region. @entity23071 expression in @entity19 injected with LV- @entity10750 Cre or LV-loxP shAQP3 alone did not differ, but consecutive injection of LV- @entity10750 Cre and LV-loxP shAQP3 significantly reduced @entity23071 expression in @entity1358 cells. However, the expression levels of @entity23071 were not altered in other cell types. Double transduction of Cre- and loxP-based lentivirus can easily generate kidney cell-specific knockdown @entity19 , and this method might be applicable to other species.

Double transduction of a Cre/LoxP lentiviral vector: a simple method to generate kidney cell-specific knockdown XXXX .

@entity19 :: ('10090', 'Species') :: ['mice'] @entity1358 :: ('MESH:D002292', 'Disease') :: ['CD', 'collecting duct'] @entity10750 :: ('15415(Tax:10090)', 'Gene') :: ['Hoxb7'] @entity23071 :: ('11828(Tax:10090)', 'Gene') :: ['AQP3']
@entity19 :: (10090,Species) :: ['mice']
