The presence of NADPH oxidase (Nox) in the kidney, especially @entity8563 , results in H2O2 production, which regulates Na(+) excretion and urine formation. Redox-sensitive transient receptor potential vanilloid 1 channels ( @entity5510 s) are distributed in mechanosensory fibers of the renal pelvis and monitor changes in intrapelvic pressure (IPP) during urine formation. The present study tested whether H2O2 derived from @entity8563 affects @entity5510 function in renal sensory responses. Perfusion of H2O2 into the renal pelvis dose dependently increased afferent renal nerve activity and substance @entity10133 ) release. These responses were attenuated by cotreatment with @entity12609 or @entity5510 blockers. In single unit recordings, H2O2 activated afferent renal nerve activity in response to rising IPP but not high salt. Western blots revealed that @entity8562 (gp91(phox)) and @entity8563 are both present in the @entity35 kidney, but @entity8563 is abundant in the renal pelvis and originates from dorsal root ganglia. This distribution was associated with expression of the @entity8563 regulators p22(phox) and polymerase   -interacting protein 2. Coimmunoprecipitation experiments showed that IPP increases polymerase   -interacting protein 2 association with @entity8563 or p22(phox) in the renal pelvis. Interestingly, immunofluorescence labeling demonstrated that @entity8563 colocalizes with @entity5510 in sensory fibers of the renal pelvis, indicating that H2O2 generated from @entity8563 may affect @entity5510 activity. Stepwise increases in IPP and saline loading resulted in H2O2 and SP release, sensory activation, diuresis, and natriuresis. These effects, however, were remarkably attenuated by Nox inhibition. Overall, these results suggest that @entity8563 -positive fibers liberate H2O2 after mechanostimulation, thereby contributing to a renal sensory nerve-mediated diuretic/natriuretic response.

H2O2 generated by NADPH oxidase 4 contributes to transient receptor potential vanilloid 1 channel-mediated mechanosensation in the XXXX kidney.

@entity10133 :: ('2155', 'Species') :: ['P (SP'] @entity8563 :: ('85431(Tax:10116)', 'Gene') :: ['Nox4'] @entity5510 :: ('83810(Tax:10116)', 'Gene') :: ['TRPV1'] @entity12609 :: ('24248(Tax:10116)', 'Gene') :: ['catalase'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity8562 :: ('66021(Tax:10116)', 'Gene') :: ['Nox2']
@entity35 :: (10116,Species) :: ['rat']
