Local injections of the neurotoxin SP-saporin into the basolateral amygdala (BLA) are reported to specifically lesion @entity16325 immunoreactive (SPR-IR) interneurons, and to reduce @entity148 related behavior. Hence, this technique might provide a means to study how defined interneuron populations regulate neuronal activity in the BLA. However, what interneuron subgroups in the BLA might be targeted by SP-saporin lesions has not been established. This study has used dual-labeling immunofluorescence in the @entity35 BLA to examine SPR-IR neurons for their colocalization with the @entity460 -binding proteins; calbindin-D28k (CB), @entity7053 (PV), and calretinin (CR); and the neuropeptides @entity1943 (SOM) and @entity10969 ( @entity10969 ). We found that all @entity10969 -IR neurons and 45% of SOM-IR interneurons expressed SPR-IR, and that 50% and 51% of the SPR-IR interneuron population expressed @entity10969 - and SOM-IR, respectively. Previous studies have reported that approximately a third of SOM-IR interneurons also express @entity10969 , which suggests a large degree of overlap between the @entity10969 , SOM and SPR expressing neurons in the BLA. We also found that the majority of SPR-IR cells were CB-IR (62%), but that these interneurons represented only 2.8% of the total CB-IR population. Moreover, SPR-IR interneurons did not express either PV-or CR- IR. Hence, SP-saporin lesions would ablate all interneurons in the BLA that contain @entity10969 , but leave the majority of the CB-IR cells intact, and have no effect on the CR- and PV-IR populations. Consequently, these results support the use of SP-saporin lesions as a useful technique to study the role of @entity10969 -IR interneurons in the BLA.

Subtypes of XXXX immunoreactive interneurons in the @entity35 basolateral amygdala.

@entity7053 :: ('25269(Tax:10116)', 'Gene') :: ['parvalbumin'] @entity10969 :: ('24604(Tax:10116)', 'Gene') :: ['neuropeptide Y', 'NPY'] @entity16325 :: ('24807(Tax:10116)', 'Gene') :: ['substance P receptor'] @entity148 :: ('MESH:D001008', 'Disease') :: ['anxiety'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity1943 :: ('MESH:D013004', 'Chemical') :: ['somatostatin']
@entity16325 :: (24807(Tax:10116),Gene) :: ['substance P receptor']
