The incidence and mortality rate of @entity14 increase every year, making it a serious threat to @entity1 health. Targeted immunogene therapy is a novel method of treating this type of @entity5 . @entity694 overexpresses @entity1501 receptor a (FRa) and @entity1501 -modified liposomes for @entity694 immunogene therapy may suppress @entity5 growth effectively. In this study, F- @entity16167 /pIL12, an FRa-targeted lipoplex loading plasmid interleukin-12 (pIL12) was prepared and its physicochemical properties were characterized. Then the antitumor effect of F- @entity16167 /pIL12 was studied in an in vivo model of @entity694 . F- @entity16167 /pIL12 was associated with about 56.6% @entity5 growth inhibition compared with the saline control. The production of @entity5 was significantly less pronounced than in controls, and there were fewer @entity5 nodules and less overall @entity5 mass (P < 0.01). There was more IL12 expression and @entity379 secretion in F- @entity16167 /pIL12-treated @entity5 tissues, but there was less FRa expression. The antitumor mechanisms involved inducing @entity5 cell apoptosis, reducing microvessel density, and stimulating @entity310 secretion. In addition, there were fewer M2 macrophages in the @entity5 microenvironment of tissues stimulated with F- @entity16167 /pIL12, which also activated the natural killer cells. H_E staining of vital organs suggested that F- @entity16167 /pIL12 is safe for use in intraperitoneally administered @entity5 therapy. It was here concluded that F- @entity16167 /plL12 may be a suitable targeting formulation for @entity694 immunogene therapy.

@entity1501 -Modified Lipoplexes Delivering the Interleukin-12 Gene for Targeting XXXX Immunogene Therapy.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1501 :: ('MESH:D005492', 'Chemical') :: ['folate'] @entity16167 :: ('5354', 'Gene') :: ['PLP'] @entity5 :: ('MESH:D009369', 'Disease') :: ['malignant ascites', 'tumor', 'cancer'] @entity310 :: ('7124', 'Gene') :: ['TNF-a'] @entity379 :: ('3458', 'Gene') :: ['IFN-y'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal cancer'] @entity694 :: ('MESH:D003110', 'Disease') :: ['CT-26 colon cancer', 'Colon cancer', 'colon cancer']
@entity694 :: (MESH:D003110,Disease) :: ['Colon Cancer']
