Agaricus blazei Murill is a native Brazilian mushroom which functions primarily as an anticancer substance in transplanted @entity19 @entity5 . However, the mechanism underlying this function of A. blazei Murill remains obscure. The present study was carried out to investigate the effect of fraction FA-2-b-ss, an RNA-protein complex isolated from A. blazei Murill, on @entity1 @entity1778 HL-60 cells in vitro. Typical apoptotic characteristics were determined by morphological methods using DNA agarose gel electrophoresis and flow cytometry. The growth suppressive effect of fraction FA-2-b-ss on HL-60 cells in vitro occurred in a dose- (5-80 microg/mL) and time-dependent (24-96 h) manner. The proliferation of HL-60 cells (1 x 10(5) cells/mL) treated with 40 microg/mL of fraction FA-2-b-ss for 24-96 h and with 5-80 microg/mL for 96 h resulted in inhibitory rates ranging from 8 to 54.5%, and from 4.9 to 86.3%, respectively. Both telomerase activity determined by TRAP-ELISA and mRNA expression of the @entity522 gene detected by RT-PCR were increased in HL-60 cells during fraction FA-2-b-ss treatment. The rate of apoptosis correlated negatively with the decrease of telomerase activity (r = 0.926, P < 0.05), but correlated positively with @entity522 mRNA expression (r = 0.926, P < 0.05). These data show that fraction FA-2-b-ss can induce HL-60 cell apoptosis and that the combined effect of down-regulation of telomerase activity and up-regulation of mRNA expression of the @entity522 gene could be the primary mechanism of induction of apoptosis. These findings provide strong evidence that fraction FA-2-b-ss could be of interest for the clinical treatment of @entity3052 .

Primary mechanism of apoptosis induction in a XXXX cell line by fraction FA-2-b-ss prepared from the mushroom Agaricus blazei Murill.

@entity1 :: ('9606', 'Species') :: ['human'] @entity522 :: ('836', 'Gene') :: ['caspase-3'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumors'] @entity3052 :: ('MESH:D015470', 'Disease') :: ['acute leukemia'] @entity1778 :: ('MESH:D007938', 'Disease') :: ['leukemia']
@entity1778 :: (MESH:D007938,Disease) :: ['leukemia']
