Although the involvement of environmental @entity286 smoke (ETS) in @entity1 @entity2 is no longer a matter of dispute, the magnitude of its impact still is. This is mainly due to the inefficiency of methodology to assess exposure to ETS especially in public places. Setting a real life exposure condition (3 h stay in local pubs) and using a matched-control study design, we quantified smoke-related DNA adducts in induced sputum and peripheral blood lymphocytes (PBL) of healthy non-smokers (n = 15) before and after a single pub visit by means of the @entity7263 -post-labeling assay. For verification, we also measured a spectrum of @entity2555 ( @entity2556 ) in the ambient air of the pubs by personal air monitors, and determined the plasma concentrations of @entity1239 and @entity4874 by gas chromatography/mass spectrometry. The ambient air concentrations of all PAH were several orders of magnitude higher than those already reported for other indoor environments. The plasma concentrations of both @entity1239 and @entity4874 increased significantly after the pub visit (P = 0.001 and P = 0.0007, respectively). Accordingly, the overall DNA adduct profile in induced sputum, but not in PBL, changed quantitatively and qualitatively after the pub visit. Of most significance was the formation of a distinct DNA adduct in induced sputum of three individuals consequent to ETS exposure. This adduct co-migrated with the standard (+/-)-anti- @entity4624 -DNA adduct, which is known to form at @entity2 mutational hotspots. We conclude that real life exposure to ETS can give rise to pro-mutagenic lesions in the lower airway, and this can be best investigated in a relevant surrogate matrix such as induced sputum.

A molecular dosimetry approach to assess @entity1 exposure to environmental XXXX smoke in pubs.

@entity1 :: ('9606', 'Species') :: ['human'] @entity286 :: ('4097', 'Species') :: ['tobacco'] @entity7263 :: ('CHEBI:37972', 'Chemical') :: ['(32)P'] @entity2555 :: ('MESH:D011084', 'Chemical') :: ['polycyclic aromatic hydrocarbons'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung cancer'] @entity1239 :: ('MESH:D009538', 'Chemical') :: ['nicotine'] @entity4874 :: ('MESH:D003367', 'Chemical') :: ['cotinine'] @entity2556 :: ('CHEBI:33848', 'Chemical') :: ['PAH'] @entity4624 :: ('CHEBI:30614', 'Chemical') :: ['benzo[a]pyrene diol epoxide']
@entity286 :: (4097,Species) :: ['tobacco']
