@entity855 -sensitive @entity972 channels ( @entity3529 ( @entity855 ) channels) are formed from an octameric complex of an inwardly rectifying @entity3529 channel ( @entity25926 , @entity14225 ) and a @entity5736 receptor ( @entity11773 , SUR2A, and SUR2B). In this study we have attempted to address the question of whether SUR heteromultimers can form using a combination of biochemical and electrophysiological approaches. We have constructed monoclonal stable lines in HEK293 cells co-expressing @entity14225 with @entity11773 and SUR2A. Using coimmunoprecipitation analysis with SUR isotype-specific antibodies two biochemical populations are distinguished, one containing @entity11773 and the other SUR2A. It is not possible to detect immune complexes containing both @entity11773 and SUR2A. Functional studies were undertaken and whole cell membrane currents were studied using the patch clamp. Concentrations of @entity5736 and @entity972 channel openers were determined that selectively inhibited or activated @entity11773 / @entity14225 and SUR2A/ @entity14225 . In the cell line expressing @entity11773 /SUR2AKir6.2 we were unable to demonstrate a population of channels with unique pharmacological properties. Thus we conclude from these studies that heteromultimeric channel complexes containing both @entity11773 and SUR2A are not formed, suggesting an incompatibility between different SUR subtypes. This incompatibility limits the pharmacological complexity of @entity3529 ( @entity855 ) channels that may be observed in native tissues.

Assembly limits the pharmacological complexity of XXXX -sensitive @entity972 channels.

@entity11773 :: ('6833', 'Gene') :: ['SUR1'] @entity3529 :: ('D011188', 'Chemical') :: ['K', 'K(+)'] @entity14225 :: ('3767', 'Gene') :: ['Kir6.2'] @entity5736 :: ('D013453', 'Chemical') :: ['sulfonylurea', 'sulfonylureas'] @entity25926 :: ('3764', 'Gene') :: ['Kir6.1'] @entity972 :: ('MESH:D011188', 'Chemical') :: ['potassium'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity855 :: (MESH:D000255,Chemical) :: ['ATP']
