@entity526 is one of the most frequent bacteria involved in uterine diseases. Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria involved in the pathogenic processes leading to postpartum metritis and @entity7288 in @entity291 . It also causes @entity32 of the endometrium. Increase of cell proliferation by LPS is part of the inflammatory process and has been reported in @entity1 epithelial and immune cells (Martin et al. 2000 J. Immunol. 165, 139-147) and from @entity291 endometrial epithelial cells (bEEC) (Guo et al. 2014 Reprod. Fertil. Dev. 26, 165-166). The aim of this study was to investigate possible changes in protein expression in relation with the proliferative response of bEEC after challenge with @entity526 -LPS. In vitro culture of bEEC was performed from 3 @entity291 . On passage 5, bEEC from each individual were exposed to 0, 8, and 16  gmL(-1) LPS for 72h. At time 0 and 72h later, attached cells were counted and for each time and LPS dosage, cells were frozen for proteomic analyses. The variation of cells number over time was analysed by ANOVA (SAS 9.1, proc GLM; SAS Institute, Inc., Cary, NC, USA). All samples were analysed (every sample run in triplicate) by 2-D gel electrophoresis coupled to matrix-assisted laser desorption/ionization-time-of-flight (MALDI- @entity1054 )/time-of-flight ( @entity1054 ) mass spectrometry (MS) and shotgun nLC-MS/MS analysis. As reported before, a significant increase in cell number was observed for cells treated with 8  gmL(-1) LPS (P <=0.001), whereas changes in cell number were highly variable and nonsignificant for 16  gmL(-1) LPS. From each sample, ~800 proteins were visualised. Results from 2-D gel coupled to MALDI- @entity1054 / @entity1054 were very reproducible (same responses between individual @entity291 ) and revealed changes in protein profiles very much related (from P<0.05 to P<0.01) to proliferative phenotypes for seven proteins. From shotgun analysis, 27 proteins were found significantly differentially expressed (P<0.05 to P<0.01) following exposure to LPS (21 up-regulated and 6 down-regulated). Among the 21 found as up-regulated, 20 were differentially expressed both for the 8 and 16  gmL(-1) LPS, whereas 5 out of 6 were down-regulated for both dosages. Differentially expressed proteins were associated to cell proliferation, apoptosis, oxidative stress, regulation of histones, @entity566 , and general cell metabolism pathways. Candidate proteins need to be confirmed from larger series of individuals and relevant pathways further studied.

159 CHANGES IN PROTEIN EXPRESSION PROFILES IN @entity291 ENDOMETRIAL EPITHELIAL CELLS (bEEC) FOLLOWING XXXX LIPOPOLYSACCHARIDE CHALLENGE.

@entity1 :: ('9606', 'Species') :: ['human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity7288 :: ('MESH:D004716', 'Disease') :: ['endometritis'] @entity566 :: ('MESH:D006967', 'Disease') :: ['allergy'] @entity1054 :: ('MESH:D000377', 'Disease') :: ['TOF'] @entity291 :: ('9913', 'Species') :: ['cows', 'cattle', 'bovine'] @entity526 :: ('562', 'Species') :: ['E. coli']
@entity526 :: (562,Species) :: ['E. COLI']
