Heat stress pretreatment of the heart is known to protect this organ against an @entity63 /reperfusion insult 24 h later. Degradation of membrane phospholipids resulting in tissue accumulation of @entity1474 , such as @entity1426 , is thought to play an important role in the multifactorial process of @entity63 /reperfusion-induced damage. The present study was conducted to test the hypothesis that heat stress mitigates the postischemic accumulation of @entity1426 in myocardial tissue, as a sign of enhanced membrane phospholipid degradation. The experiments were performed on hearts isolated from @entity35 either 24 h after total body heat treatment (42 degrees C for 15 min) or 24 h after sham treatment (control). Hearts were made @entity63 for 45 min and reperfused for another 45 min. Heat pretreatment resulted in a significant improvement of postischemic hemodynamic performance of the isolated @entity35 hearts. The release of @entity908 kinase was reduced from 30 +/- 14 (control group) to 17 +/- 5 units/g wet wt per 45 min (heat-pretreated group) (p < or = 0.05). Moreover, the tissue content of the inducible heat stress protein @entity18273 was found to be increased 3-fold 24 h after heat treatment. Preischemic tissue levels of @entity1426 did not differ between heat-pretreated and control hearts. The postischemic ventricular content of @entity1426 was found to be significantly reduced in heat-pretreated hearts compared to sham-treated controls (6.6 +/- 3.3. vs. 17.8 +/- 12.0 nmol/g wet wt). The findings suggest that mitigation of membrane phospholipid degradation is a potential mechanism of heat stress-mediated protection against the deleterious effects of @entity63 and reperfusion on cardiac cells.

Heat stress pretreatment mitigates postischemic XXXX accumulation in @entity35 heart.

@entity63 :: ('MESH:D007511', 'Disease') :: ['ischemia', 'ischemic'] @entity908 :: ('MESH:D003401', 'Chemical') :: ['creatine'] @entity18273 :: ('266759(Tax:10116)', 'Gene') :: ['HSP70'] @entity1426 :: ('MESH:D016718', 'Chemical') :: ['arachidonic acid'] @entity1474 :: ('MESH:D005231', 'Chemical') :: ['polyunsaturated fatty acids'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats']
@entity1426 :: (MESH:D016718,Chemical) :: ['arachidonic acid']
