OBJECTIVES: Radiation therapy is a common treatment strategy for @entity189 . However, radiation is not without adverse side effects, especially toward healthy vocal fold tissue, which can lead to long-term impairments in vocal function. The objective of this preliminary study was to investigate early responses of healthy @entity1 @entity3746 ( @entity3746 ) to radiation. METHODS: @entity3746 were exposed to a single or fractionated dose radiation scheme. Nonradiated @entity3746 served as controls. Morphology of radiated and control @entity3746 was subjectively examined. Quantitative polymerase chain reaction was used to evaluate the effect of radiation on extracellular matrix and inflammatory-related genes. @entity3746 viability was investigated using a LIVE/DEAD and clonogenic assay. RESULTS: Single or fractioned dose radiated @entity3746 were morphologically indistinguishable from control @entity3746 . No significant differences in gene expression were observed following either radiation scheme and as compared to controls. Clonogenic assay revealed @entity3746 following the fractionated but not single dose scheme. No changes in viability were detected using the LIVE/DEAD assay. CONCLUSIONS: We present one of the first investigations to evaluate early responses of healthy @entity3746 to radiation. Findings will contribute to a growing body of literature seeking to elucidate the biological mechanisms underlying voice changes following radiation therapy for @entity189 .

Early Cellular Response to Radiation in XXXX Vocal Fold Fibroblasts.

@entity1 :: ('9606', 'Species') :: ['human'] @entity3746 :: ('MESH:D014826', 'Disease') :: ['vocal fold fibroblasts', 'reduced VFF viability', 'VFF'] @entity189 :: ('MESH:D002277', 'Disease') :: ['laryngeal carcinoma']
@entity1 :: (9606,Species) :: ['Human']
