BACKGROUND: The opportunistic pathogen @entity7408 is a member of the Saccharomycetaceae @entity375 . Like its close relative @entity375 , it underwent a whole-genome duplication followed by an extensive loss of genes. Its genome contains a large number of very long tandem repeats, called megasatellites. In order to determine the whole replication program of the @entity7408 genome and its general chromosomal organization, we used deep-sequencing and chromosome conformation capture experiments. RESULTS: We identified 253 replication fork origins, genome wide. Centromeres, @entity24364 and HMR loci, and most histone genes are replicated early, whereas natural chromosomal breakpoints are located in late-replicating regions. In addition, 275 autonomously replicating sequences (ARS) were identified during ARS-capture experiments, and their relative fitness was determined during growth competition. Analysis of ARSs allowed us to identify a 17-bp consensus, similar to the @entity375 ARS consensus sequence but slightly more constrained. Megasatellites are not in close proximity to replication origins or termini. Using chromosome conformation capture, we also show that early origins tend to cluster whereas @entity42339 do not cluster in the @entity375 nucleus. CONCLUSIONS: Despite a shorter cell cycle, the @entity7408 replication program shares unexpected striking similarities to @entity375 , in spite of their large evolutionary distance and the presence of highly repetitive large tandem repeats in @entity7408 . No correlation could be found between the replication program and megasatellites, suggesting that their formation and propagation might not be directly caused by replication fork initiation or termination.

Genome-wide replication landscape of XXXX .

@entity7408 :: ('5478', 'Species') :: ['C. glabrata', 'Candida glabrata'] @entity375 :: ('4932', 'Species') :: ['yeast', 'Saccharomyces cerevisiae', 'yeasts', 'S. cerevisiae'] @entity42339 :: ('MESH:C563043', 'Disease') :: ['non-subtelomeric megasatellites'] @entity24364 :: ('MESH:C564972', 'Disease') :: ['HML']
@entity7408 :: (5478,Species) :: ['Candida glabrata']
