@entity3610 ( @entity3610 ) is a @entity74 characterized by arrested follicular development prior to selection of a dominant follicle. Dominant follicles produce large amounts of @entity53 but @entity3610 follicles do not. With several potential @entity18946 ( @entity4819 ) inhibitors in follicular fluid, the question arises whether @entity4819 is expressed in @entity3610 granulosa cells, but the activity is inhibited, or whether @entity4819 is not expressed in @entity3610 . The purpose of the present study was to determine whether @entity4819 mRNA expression is altered in @entity3610 and to correlate @entity4819 mRNA expression in individual follicles with @entity18946 stimulatory bioactivity and @entity53 in the follicular microenvironments. @entity4819 mRNA was measured in individual follicles from 16 @entity3610 and 48 regularly cycling control @entity1 by quantitative polymerase chain reaction (PCR) and correlated with follicular fluid @entity53 concentrations and @entity18946 stimulating bioactivity measured by the @entity35 granulosa cells @entity18946 bioassay. Follicular fluid @entity53 was low in all control follicles <7 mm in diameter. Some follicles > or = 7 mm contained elevated @entity53 values (P < 0.01) and all had an @entity2671 : @entity53 ratio of <4. Only in granulosa cells from follicles > or = 7 mm with an @entity2671 : @entity53 ratio of <4 were @entity4819 mRNA levels increased (P < 0.05). These same follicles also contained increased levels of @entity18946 stimulating bioactivity whereas follicles <7 mm or with @entity2671 : @entity53 ratio of >4 contained little or no bioactivity. All @entity3610 follicles contained low levels of @entity53 , @entity4819 mRNA and @entity18946 stimulating bioactivity similar to size-matched control follicles. These data indicate that @entity4819 mRNA expression and @entity53 production begin in developing follicles when they reach approximately 7 mm in diameter. @entity53 production is low in @entity3610 follicles because there is insufficient @entity18946 stimulating bioactivity to increase @entity4819 mRNA expression.

XXXX mRNA expression in individual follicles from @entity3610 .

@entity1 :: ('9606', 'Species') :: ['women'] @entity3610 :: ('MESH:D011085', 'Disease') :: ['Polycystic ovary syndrome', 'PCOS'] @entity4819 :: ('1588', 'Gene') :: ['P450AROM'] @entity53 :: ('MESH:D004958', 'Chemical') :: ['oestradiol', 'Oestradiol'] @entity74 :: ('MESH:D030342', 'Disease') :: ['common reproductive disorder'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity18946 :: ('25147(Tax:10116)', 'Gene') :: ['aromatase'] @entity2671 :: ('MESH:D000735', 'Chemical') :: ['androstenedione']
@entity18946 :: (25147(Tax:10116),Gene) :: ['Aromatase']
