Females of the hematophagous mosquito species require a vertebrate blood meal to supply @entity3534 and other nutrients necessary for egg development, serving as the driving force for the spread of many @entity4002 in @entity1 . Blood digestion utilizes both early and late phase @entity563 proteases (SPs) that are differentially regulated at the transcriptional and post-transcriptional level. To uncover the regulatory complexity of SPs in the female mosquito midgut, we investigated involvement of miRNAs in regulating the juvenile hormone (JH)-controlled chymotrypsin-like SP, JHA15. We identified regulatory regions complementary to the mosquito-specific miRNA, miR-1890, within the 3' UTR of JHA15 mRNA. The level of the JHA15 transcript is highest post eclosion and drastically declines post blood meal (PBM), exhibiting an opposite trend to miR-1890 that peaks at 24  h PBM. Depletion of miR-1890 results in defects in blood digestion, ovary development and egg deposition. JHA15 mRNA and protein levels are elevated in female mosquitoes with miR-1890 inhibition. JHA15 RNA interference in the miR-1890 depletion background alleviates miR-1890 depletion phenotypes. The miR-1890 gene is activated by the @entity11088 pathway that involves the @entity4080 receptor and the early genes, E74B and Broad Z2. Our study suggests that miR-1890 controls JHA15 mRNA stability in a stage- and tissue- specific manner.

Mosquito-specific microRNA-1890 targets the juvenile hormone-regulated XXXX protease JHA15 in the female mosquito gut.

@entity3534 :: ('MESH:D000596', 'Chemical') :: ['amino acids'] @entity4002 :: ('MESH:D011778', 'Disease') :: ['vector-borne diseases'] @entity1 :: ('9606', 'Species') :: ['humans'] @entity11088 :: ('MESH:D004441', 'Chemical') :: ['20-hydroxyecdysone'] @entity4080 :: ('MESH:D004440', 'Chemical') :: ['ecdysone'] @entity563 :: ('CHEBI:17822', 'Chemical') :: ['serine']
@entity563 :: (CHEBI:17822,Chemical) :: ['serine']
