Targeted antiangiogenic gene therapy is an attractive approach to treat @entity5 . However, the relative paucity of the receptors of the commonly used adenovirus serotype 5 in endothelial cells as compared with liver cells undermines the use of this vector for targeting the endothelial cells in @entity5 . To overcome this problem, we analyzed the ability of a hybrid @entity11650 /35 virus, where the serotype 5 fiber has been replaced with the fiber from serotype 35, to target @entity5 vasculature. Infection of @entity1 umbilical vein endothelial cells (HUVECs) with @entity11650 /35 at MOI 120 infected 100% of cells. In contrast, infection with @entity11650 at the same MOI infected only 10% HUVECs. @entity11650 /35 was even more effective in transducing @entity1 aortic endothelial cells (HAECs), as infection with @entity11650 /35 at MOI 3.6 was sufficient to transduce 95% of cells. Gene expression analyses demonstrated that infection of HUVECs and HAECs with @entity11650 /35 resulted in between 1 and 3 orders of magnitude higher gene expression than infection with @entity11650 . Furthermore, various liver-derived cells were less infectable with @entity11650 /35 than @entity11650 , indicating a favorable @entity137 profile for this virus. In a @entity35 colon @entity5 model, @entity11650 was located mainly in the @entity409 after hepatic artery administration. In contrast, @entity11650 was found only in the angiogenesis-rich border region of the @entity5 . Double immunostaining revealed that @entity11650 /35 colocalized with @entity2279 and @entity319 positive endothelial cells. These results indicate that @entity11650 may be useful in anticancer strategies targeting @entity5 endothelial cells.

Efficient infection of XXXX endothelial cells by a capsid-modified adenovirus.

@entity1 :: ('9606', 'Species') :: ['human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity11650 :: ('8081', 'Gene') :: ['Ad5', 'Ad5/35'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'carcinoma tumor', 'metastatic cancer', 'tumors'] @entity409 :: ('MESH:D008107', 'Disease') :: ['liver parenchyma'] @entity2279 :: ('5175', 'Gene') :: ['CD31'] @entity319 :: ('3791', 'Gene') :: ['Flk-1'] @entity35 :: ('10116', 'Species') :: ['rat']
@entity5 :: (MESH:D009369,Disease) :: ['tumor']
