Limit test of @entity4212 in @entity10085 ginkgolides @entity1559 injection materials by UV-Vis and HPLC-DAD method was studied in this essay. The HPLC-DAD method has lower LOD (about 1% of the UV-Vis), that is, the sensitivity is higher than UV-Vis method. Through the analysis of the kinds of @entity3088 ingredients in the samples by LC-MS, the three compounds with highest contents are @entity15605 , @entity3092 and @entity58010 . @entity15605 , @entity3092 and @entity58010 were chosen as reference compounds for HPLC analysis, and the HPLC separation analysis was carried on an Agilent Eclipse plus @entity6173 column (4.6 mm x 250 mm, 5  m) with @entity1042 and water containing 0.4% @entity8718 (50: 50) as mobile phase, and the flow rate was 1.0 mL x @entity1009 ). The detection wavelength was set at 360 nm. This method has good specificity, precision and reproducibility. The LODs of @entity3092 , @entity40194 and @entity58010 were 27.6, 22.3, 29.5  g x L(-1). The average recovery was 87.9% (RSD 3.3%), 91.7% (RSD 3.1%), 88.3 (RSD 1.3%) for @entity3092 , @entity40194 and @entity58010 , respectively. Based on the 10 batches of sample results and sensitivity of different HPLC, the content of total @entity3088 ingredients of @entity10085 ginkgolides @entity1559 injection materials was limited no more than 2 x 10(-5). This method is simple, quick and has good maneuverability, and could be used to the limit test of @entity3088 in the @entity10085 ginkgolides @entity1559 injection materials.

[Study on limit detection of XXXX in @entity10085 ginkgolides @entity1559 injection materials by LC-MS and HPLC-DAD].

@entity8718 :: ('MESH:C030242', 'Chemical') :: ['phosphoric acid'] @entity15605 :: ('MESH:C006552', 'Chemical') :: ['kaempferol', 'Kaempferol'] @entity1042 :: ('MESH:D000432', 'Chemical') :: ['methanol'] @entity4212 :: ('MESH:D047309', 'Chemical') :: ['flavones'] @entity58010 :: ('MESH:C047368', 'Chemical') :: ['isorhamnetin'] @entity10085 :: ('CHEBI:35190', 'Chemical') :: ['diterpene'] @entity1009 :: ('966', 'Gene') :: ['min(-1'] @entity1559 :: ('MESH:D008536', 'Chemical') :: ['meglumine'] @entity3088 :: ('MESH:D005419', 'Chemical') :: ['flavonoids'] @entity6173 :: ('27241', 'Gene') :: ['C18'] @entity40194 :: ('MESH:C449720', 'Chemical') :: ['kaempferide'] @entity3092 :: ('MESH:D011794', 'Chemical') :: ['quercetin']
@entity4212 :: (MESH:D047309,Chemical) :: ['flavones']
