Background: Pulsed radiofrequency (PRF) has been widely used to treat @entity1118 , but the effectiveness and mechanisms in preventing early @entity9 have not been well explored. Even fewer knowledge is available in its impact on glia-mediated nociceptive sensitization. This study aims to elucidate the modulation of PRF on @entity128 -induced @entity158 development and activation of spinal mitogen-activated protein kinases (MAPKs). Methods: In a @entity35 spinal nerve ligation (SNL) model, a low-volt PRF treatment was applied to the L5 dorsal root ganglion after @entity128 . Nociceptive behaviours were measured by von Frey and heat withdrawal tests at multiple time points. MAPK activations, including p-ERK and p- @entity12220 , as well as @entity1505 -   level in the spinal dorsal horn were assessed and the cell types that expressed MAPK activation were identified by double immuno fluorescence staining.Results: We found that SNL promptly induced @entity9 in the affected hind limb for over 1 week as well as increased p-ERK and p- @entity12220 in the spinal dorsal horn. PRF significantly attenuated SNL-induced mechanical @entity1931 and thermal @entity1931 for 5   7 days. PRF also inhibited ERK and @entity12220 activations, which were found majorly located within neurons and microglia, respectively. Besides, PRF significantly suppressed expression of @entity1505 -   in the spinal dorsal horn throughout the course. Conclusions: Low-volt PRF significantly ameliorated SNL-induced acute @entity158 . Inferentially, PRF may inhibit spinal sensitization by down-regulating spinal MAPK activations and activation-mediated cytokine release.We demonstrated that early PRF treatment in @entity697 helps to ameliorate @entity9 development.

Pulsed radiofrequency inhibited activation of spinal mitogen-activated protein kinases and ameliorated early @entity9 in XXXX .

@entity697 :: ('MESH:D000208', 'Disease') :: ['acute nerve injury'] @entity1931 :: ('MESH:D006930', 'Disease') :: ['allodynia', 'hyperalgesia'] @entity1505 :: ('24835(Tax:10116)', 'Gene') :: ['TNF'] @entity128 :: ('MESH:D020209', 'Disease') :: ['nerve injury'] @entity158 :: ('MESH:D010146', 'Disease') :: ['pain'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity12220 :: ('81649(Tax:10116)', 'Gene') :: ['p38'] @entity1118 :: ('MESH:D059350', 'Disease') :: ['chronic pain'] @entity9 :: ('MESH:D009437', 'Disease') :: ['neuropathic pain']
@entity35 :: (10116,Species) :: ['rats']
