The translocator protein (TSPO), previously designated as peripheral-type @entity1245 receptor, is an evolutionary conserved protein that is found in many Eukarya, Archae, and Bacteria, in which it plays several important functions including for example membrane biogenesis, signaling, and stress response. A tspo homolog gene has been identified in several members of the @entity11763 genus, among which the soil bacterium P. fluorescens @entity47533 . In this bacterium, the tspo gene is located in the vicinity of a putative hybrid @entity3793 kinase-encoding gene. Since tspo has been involved in water stress related response in plants, we explored the effects of hyperosmolarity and temperature on @entity47533 tspo expression using a strategy based on lux-reporter fusions. We show that the two genes Pfl01_2810 and tspo are co-transcribed forming a transcription unit. The expression of this operon is growth phase-dependent and is increased in response to high concentrations of @entity4202 , @entity969 and to a @entity12210 treatment, which are conditions leading to activity of the major cell wall stress responsive extracytoplasmic sigma factor AlgU. Interestingly, the promoter region activity is strongly lowered in a @entity796 algU mutant, suggesting that AlgU may be involved at least partly in the molecular mechanism leading to Pfl01_2810-tspo expression. In silico analysis of this promoter region failed to detect an AlgU consensus binding site; however, a putative binding site for the @entity1457 response RpoH sigma factor was detected. Accordingly, the promoter activity of the region containing this sequence is increased in response to high growth temperature and slightly lowered in a @entity796 rpoH mutant strain. Taken together, our data suggest that @entity11763 tspo gene may belong at least partly to the cell wall stress response.

Expression of the translocator protein (TSPO) from XXXX requires the stress regulatory sigma factors AlgU and RpoH.

@entity796 :: ('287', 'Species') :: ['P. aeruginosa'] @entity11763 :: ('294', 'Species') :: ['P. fluorescens', 'Pseudomonas'] @entity3793 :: ('CHEBI:27570', 'Chemical') :: ['histidine'] @entity1457 :: ('MESH:D012769', 'Disease') :: ['heat shock'] @entity47533 :: ('205922', 'Species') :: ['Pf0-1', 'P. fluorescens Pf0-1'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity1245 :: ('MESH:D001569', 'Chemical') :: ['benzodiazepine'] @entity12210 :: ('CHEBI:74159', 'Chemical') :: ['D-cycloserine'] @entity4202 :: ('D012965', 'Chemical') :: ['NaCl']
@entity47533 :: (205922,Species) :: ['Pseudomonas fluorescens Pf0-1']
