UNASSIGNED: Embryonic stem cells (ESCs) are pluripotent stem cells derived from early stage embryos. It remains unclear whether inhibiting the Wnt/b  -catenin signaling pathway using dickkopf Wnt signaling pathway inhibitor  1 ( @entity26735 ) impacts on the differentiation potential of @entity19 ESCs in  vitro and in  vivo. In the present study, immunohistochemical staining was used to measure the expression of markers of the three germ layers in ESCs and @entity44 derived from ESCs. The expression of markers for the Wnt/b  -catenin signaling pathway were detected by reverse transcription  -polymerase chain reaction (RT  -qPCR). Immunohistochemistry and western blotting indicated that the expression levels of octamer  -binding transcription factor  4 in the @entity26735  -treated ESC group were significantly greater compared with the control ESCs. Reduced expression levels of @entity40426 and @entity7739  were observed in the @entity26735  -treated ESCs and @entity44 derived from @entity26735  -treated ESCs compared with the control group. Increased expression levels of @entity25235 were observed in the @entity26735  -treated ESCs compared with the control group. RT  -qPCR indicated that b  -catenin expression was significantly reduced in @entity26735  -treated ESCs and @entity44 derived from @entity26735  -treated ESCs compared with the control groups. Western blotting indicated no alterations in the expression of @entity6913 , however, the levels of phosphorylated  - @entity6913 were significantly greater in the @entity26735 treatment groups, while @entity1896 and c  -Myc expression levels were significantly reduced in the @entity26735 treatment groups compared with the control groups. These results suggest that inhibiting Wnt signaling in ESCs using @entity26735 may promote @entity19 ESCs to differentiate into endoderm in  vitro and in  vivo, and suppress the tumorigenicity of ESCs.

Dickkopf Wnt signaling pathway inhibitor  1 regulates the differentiation of XXXX embryonic stem cells in vitro and in vivo.

@entity1896 :: ('12443(Tax:10090)', 'Gene') :: ['cyclin  D1'] @entity6913 :: ('56637(Tax:10090)', 'Gene') :: ['GSK  -3b'] @entity25235 :: ('20671(Tax:10090)', 'Gene') :: ['SOX17'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity40426 :: ('18012(Tax:10090)', 'Gene') :: ['NeuroD'] @entity26735 :: ('13380(Tax:10090)', 'Gene') :: ['DKK1'] @entity44 :: ('MESH:D013724', 'Disease') :: ['teratomas'] @entity7739 :: ('12159(Tax:10090)', 'Gene') :: ['bone morphogenetic protein  4']
@entity19 :: (10090,Species) :: ['mouse']
