@entity3252 is a @entity6766 caused by @entity3204 , an obligate intracellular parasite. The parasite remains protected within a parasitophorous vacuole (PV), a specialized compartment formed within the infected host cell during and after invasion. Dense granules (GRA) are @entity3204 specialized secretory organelles involved in PV development. GRA2 contributes to the formation of intravacuolar network in the PV, allowing nutrients transportation to nourish the parasites. GRA5 helps to inhibit apoptosis of the infected cells thereby protecting the parasites. As such, these two essential antigens have been selected as the target subjects. Heterologous expression in @entity13724 pLysS (DE3) of GRA2 and GRA5 fragment was achieved by transfecting with recombinant expression GRA2- and GRA5-pRSET B plasmid, respectively. His-tagged recombinant proteins were affinity purified using a @entity3406 - @entity49470 column. The identities of recombinant rGRA2 (30 kDa) and 5 (20 kDa) proteins were confirmed by western blotting using immune serum from a @entity1 with @entity3252 and by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. The purified T. gondii antigens provide candidates for future development of diagnostic kits of @entity1 @entity281 as well as vaccines.

HETEROLOGOUS EXPRESSION OF XXXX DENSE GRANULE PROTEIN 2 AND 5.

@entity3406 :: ('MESH:D009532', 'Chemical') :: ['Nickel'] @entity1 :: ('9606', 'Species') :: ['human', 'patient'] @entity6766 :: ('MESH:D005517', 'Disease') :: ['foodborne disease'] @entity49470 :: ('MESH:D009571', 'Chemical') :: ['nitrilotriacetic acid'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity3252 :: ('MESH:D014123', 'Disease') :: ['toxoplasmosis', 'Toxoplasmosis'] @entity13724 :: ('511693', 'Species') :: ['E. coli BL21'] @entity3204 :: ('5811', 'Species') :: ['T. gondii', 'Toxoplasma gondii']
@entity3204 :: (5811,Species) :: ['TOXOPLASMA GONDII']
