Fluorescence spectroscopy offers an effective, noninvasive approach to the detection of precancers in multiple organ sites. Clinical studies have demonstrated that fluorescence spectroscopy can provide highly sensitive, specific and cost-effective diagnosis of cervical precancers. However, the underlying biochemical mechanisms responsible for differences in the fluorescence spectra of normal and @entity6349 tissue are not fully understood. We designed a study to assess the differences in autofluorescence of normal and @entity6349 cervical tissue. Transverse, fresh tissue sections were prepared from colposcopically normal and abnormal biopsies in a 34- @entity1 study. Autofluorescence images were acquired at 380 and 460 nm excitation. Results showed statistically significant increases in epithelial fluorescence intensity (arbitrary units) at 380 nm excitation in @entity6349 tissue (106 +/- 39) relative to normal tissue (85 +/- 30). The fluorophore responsible for this increase is possibly reduced @entity2125 . Stromal fluorescence intensities in the @entity6349 samples decreased at both 380 nm (102 +/- 34 [ @entity1141 ] vs 151 +/- 44 [normal]) and 460 nm excitation (93 +/- 35 [ @entity1141 ] vs 137 +/- 49 [normal]), wavelengths at which collagen is excited. Decreased redox ratio (17-40% reduction) in @entity6349 tissue sections, indicative of increased metabolic activity, was observed in one-third of the paired samples. These results provide valuable insight into the biological basis of the differences in fluorescence of normal and precancerous cervical tissue.

Autofluorescence microscopy of fresh cervical-tissue sections reveals alterations in tissue biochemistry with XXXX .

@entity1 :: ('9606', 'Species') :: ['patient'] @entity2125 :: ('MESH:D009243', 'Chemical') :: ['nicotinamide adenine dinucleotide'] @entity6349 :: ('MESH:D004416', 'Disease') :: ['dysplastic'] @entity1141 :: ('MESH:C535662', 'Disease') :: ['dysplasia']
@entity1141 :: (MESH:C535662,Disease) :: ['dysplasia']
