BACKGROUND: The detection of autoantibodies to the TSH-receptor (TRAb) by radio-receptor assays (RRA) is widely requested in clinical practice for the diagnostic workup of @entity2743 and its differentiation from diffuse thyroid autonomy. Additionally, TRAb measurement can be useful during antithyroid drug treatment of @entity2743 to evaluate the risk of relapse after therapy discontinuation. Nevertheless, some @entity1 affected by @entity2743 are TRAb-negative when 1st generation assay is used. METHODS: In this study we evaluated the diagnostic performance of a newly developed 2nd generation TRAb assay (TRAK @entity1 DYNOtest, BRAHMS Diagnostica GmbH, Berlin, Germany) in 74 untreated @entity1 affected by @entity2743 , 53 untreated @entity1 affected by @entity2621 and 88 @entity1 affected by @entity8170 . We also compared the new TRAb assay with the 1st generation test (TRAK Assay, BRAHMS Diagnostica GmbH, Berlin, Germany) and anti-thyroperoxidase assay (AbTPO DYNOtest, BRAHMS Diagnostica GmbH, Berlin). RESULTS: The 2nd generation TRAb assay showed the better diagnostic sensitivity in @entity2743 (97%) with respect to the 1st generation assay (85%) and AbTPO assay (64%). The AbTPO assay was positive in 50 of 53 (94%) @entity1 affected by @entity1669 . The 1st and 2nd generation TRAb assays were positive in 4 (7%) and 7 (13%) of 53 @entity1 affected by @entity1669 , respectively. No @entity1 affected by @entity8170 showed positive 1st and 2nd generation TRAb assay while AbTPO levels were positive in 8 of 88 @entity1 (specificity 91%). CONCLUSIONS: In conclusion, the 2nd generation TRAb assay is clearly more sensitive than the 1st generation test and should be used in clinical practice to minimize the incidence of TRAb-negative @entity2743 . Long term prospective studies are needed to evaluate the prognostic role of 2nd generation TRAb assay in @entity2743 . The assay of AbTPO is the best marker for @entity1669 but is clearly less sensitive than 1st and 2nd generation TRAb assays in @entity2743 . Consequently, AbTPO assay should not be performed in @entity2743 neither alone or in association with TRAb.

Evaluation of the 2nd generation radio-receptional assay for anti-TSH receptor antibodies (TRAb) in XXXX . Comparison with 1st generation and anti-thyroperoxidase antibodies (AbTPO).

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity8170 :: ('MESH:D006044', 'Disease') :: ['euthyroid nodular goiter', 'nodular goiter'] @entity2743 :: ('MESH:D006111', 'Disease') :: ["Graves' disease"] @entity2621 :: ('MESH:D050031', 'Disease') :: ["Hashimoto's thyroiditis"] @entity1669 :: ('MESH:D013967', 'Disease') :: ['autoimmune thyroiditis']
@entity1669 :: (MESH:D013967,Disease) :: ['autoimmune thyroid diseases']
