The intracellular signal transduction controlling the proliferation of osteoblastic cells on a thin film of poorly crystalline @entity78 apatite crystals ( @entity4901 ) was studied in vitro. The @entity4901 thin film was prepared on @entity852 culture dishes or cover glasses using @entity1391 -buffered @entity460 ion solution, which was made oversaturated by heating an undersaturated solution prepared at low temperature. The @entity4901 thin film was used for cell culture without additional surface treatment. Several differences were found between the cells plated on a cell culture dish and the cells cultured on the @entity4901 surface. Entry into S-phase of the cell cycle was markedly delayed and there was a low proliferation rate of osteoblast. On the @entity4901 thin film, the cells spread in a more slender shape. Also, the formation of focal adhesions and stress fibers, examined using immunocytochemical staining, was strikingly weaker in the cells on @entity4901 . The activation of focal adhesion kinase (FAK) was low as well. Expression of cyclins @entity12840 was also lower. The @entity3963 ( @entity3963 )-MAP kinase signaling pathway was weakly activated by stimulation with serum. These results demonstrate that the low cell proliferation on the @entity4901 surface appears to be due to insufficient activation of signaling that forces the cell cycle to progress and this may be due to weak adhesion signaling in cells on that surface.

The role of cell signaling defects on the proliferation of osteoblasts on the XXXX apatite thin film.

@entity852 :: ('MESH:D011137', 'Chemical') :: ['polystyrene'] @entity12840 :: ('25802', 'Gene') :: ['D1 and E'] @entity78 :: ('MESH:C020243', 'Chemical') :: ['calcium phosphate'] @entity3963 :: ('5594', 'Gene') :: ['Ras-Extracellular signal-regulated kinase', 'ERK'] @entity1391 :: ('CHEBI:26020', 'Chemical') :: ['phosphate'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity4901 :: ('CHEBI:36751', 'Chemical') :: ['PCA']
@entity78 :: (MESH:C020243,Chemical) :: ['calcium phosphate']
