@entity49355 . michiganensis (Cmm) is a Gram-positive seed-transmitted bacterial phytopathogen responsible for substantial economic losses by adversely affecting @entity523 production worldwide. A high-throughput, cell-based screen was adapted to identify novel small molecule growth inhibitors to serve as leads for future bactericide development. A library of 4,182 compounds known to be bioactive against @entity375 was selected for primary screening against Cmm wild-type strain C290 for whole-cell growth inhibition. Four hundred sixty-eight molecules (11.2% hit rate) were identified as bacteriocidal or bacteriostatic against Cmm at 200  M. Seventy-seven candidates were selected based on Golden Triangle analyses for secondary screening. Secondary screens showed that several of these candidates were strain-selective. Several compounds were inhibitory to multiple Cmm strains as well as @entity3881 , but not to @entity11763 , Mitsuaria sp., Lysobacter enzymogenes, @entity10263 , @entity14320 , or @entity526 . Most of the compounds were not phytotoxic and did not show overt host @entity137 . Using a novel 96-well bioluminescent Cmm seedling infection assay, we assessed effects of selected compounds on pathogen infection. The 12 most potent novel molecules were identified by compiling the scores from all secondary screens combined with the reduction of pathogen infection in planta. When tested for ability to develop resistance to the top-12 compounds, no resistant Cmm were recovered, suggesting that the discovered compounds are unlikely to induce resistance. In conclusion, here we report top-12 compounds that provide chemical scaffolds for future Cmm-specific bactericide development.

Discovery of novel small molecule modulators of XXXX subsp. michiganensis.

@entity14320 :: ('28025', 'Species') :: ['Bifidobacterium animalis'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity11763 :: ('294', 'Species') :: ['Pseudomonas fluorescens'] @entity10263 :: ('47715', 'Species') :: ['Lactobacillus rhamnosus'] @entity523 :: ('4081', 'Species') :: ['tomato'] @entity49355 :: ('28447', 'Species') :: ['Clavibacter michiganensis subsp'] @entity375 :: ('4932', 'Species') :: ['Saccharomyces cerevisiae'] @entity3881 :: ('1423', 'Species') :: ['Bacillus subtilis'] @entity526 :: ('562', 'Species') :: ['Escherichia coli']
@entity49355 :: (28447,Species) :: ['Clavibacter michiganensis']
