OBJECTIVES: We aimed to determine the biochemical and histopathologic effects of direct @entity26 supply to the preservation fluid of static cold storage system with a simple method on @entity35 livers. MATERIALS AND METHODS: Sixteen @entity35 were randomly divided into 2 groups: the control group, which contained Ringer's @entity880 as preservation fluid; and the @entity26 group, which contained @entity26 and Ringer's @entity880 for preservation. Each liver was placed in a bag containing 50 mL Ringer's @entity880 and placed in ice-filled storage containers. One hundred percent @entity26 supplies were given via a simple, inexpensive system created in our laboratory, to the livers in @entity26 group. We obtained samples for histopathologic evaluation in the 12th hour. In addition, 3 mL of preservation fluid was subjected to biochemical analysis at 0, sixth, and twelfth hours. @entity5906 aminotransferase, @entity3929 aminotransferase, @entity880 dehydrogenase, and pH levels were measured from the preservation fluid. RESULTS: In @entity26 -supplemented group, the acceleration speed of increase in @entity3929 aminotransferase and @entity880 dehydrogenase levels at sixth hour and @entity880 dehydrogenase, @entity3929 aminotransferase, and @entity880 dehydrogenase levels at 12th hour were statistically significantly reduced. In histopathologic examination, all parameters except ballooning were statistically significantly better in the @entity26 -supplemented group. CONCLUSIONS: This simple system for oxygenation of liver tissues during static cold storage was shown to be effective with good results in biochemical and histopathologic assessments. Because this is a simple, inexpensive, and easily available method, larger studies are warranted to evaluate its effects (especially in @entity1 ).

The Effects of Direct @entity26 Supply During Static Cold Preservation of XXXX Livers: An Experimental Study.

@entity1 :: ('9606', 'Species') :: ['humans'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity5906 :: ('D001224', 'Chemical') :: ['Aspartate'] @entity3929 :: ('D000409', 'Chemical') :: ['alanine'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats']
@entity35 :: (10116,Species) :: ['Rat']
