OBJECTIVE: To investigate the primary culture and adipogenic process of pre-adipocytes from infrapatellar fat pad of osteoarthritic @entity1 . METHODS: The pre-adipocytes were isolated by enzymatic digestion. The morphological changes of cultured cells were observed and the growth curve was drawn by CCK-8 method. During the adipogenic process, the intracytoplasmic lipid of differentiated cells was determined by oil red O staining. And the @entity1254 levels in the culture supernatants were measured by ELISA (enzyme-linked immunosorbent assay). RESULTS: The primary cultured fibroblast-like cells were spindle-shaped. In the process of adipogenesis, the intracytoplasmic lipid droplets were observed at Day 3 and over 80% of the cells differentiated into adipocytes at Day 21. With the increasing number of adipocytes, the @entity1254 levels in the culture supernatant elevated and peaked at Week 3. The differentiated cells were proven to be adipocytes functioning actively. CONCLUSION: The primary culture and adipogenic process of pre-adipocytes in infrapatellar fat pad of osteoarthritic @entity1 has been successfully established. Thus it may provide an ideal model for the study of endocrine function of infrapatellar fat pad and understanding its role in the pathogenesis of @entity571 .

[Primary culture and adipogenic process of pre-adipocytes from infrapatellar fat pad of osteoarthritic XXXX ].

@entity1 :: ('9606', 'Species') :: ['patients'] @entity1254 :: ('9370', 'Gene') :: ['adiponectin'] @entity571 :: ('MESH:D010003', 'Disease') :: ['osteoarthritis']
@entity1 :: (9606,Species) :: ['patients']
