Subepithelial @entity82 in @entity565 involves an increase in the thickening of the lamina reticularis and is due to increased deposition of collagen I, III and V, and fibronectin. The cause of the thickening of the reticular layer is not known in detail, however, it is proposed to be caused by bronchial myofibroblasts. The transformation of fibroblasts to myofibroblasts may be contributed by inflammatory cytokines. In this paper we have studied and compared in vivo tissue material with a @entity1 fibroblast target cell. A normal primary fetal fibroblast cell line and @entity8173 ( @entity1 fibroblast lurg cells) were used as a comparison between fibroblasts from @entity1 central biopsies regarding morphology and cell proliferation. Both cell morphology and cell proliferation rate was similar between the different set of cell cultures. Furthermore, it could be concluded that fibroblasts cultures from @entity1 with @entity565 were surrounded by more extracellular matrix molecules compared to the primary cell line @entity8173 , which may mimic the in vivo situation during formation of @entity82 . We wanted to investigate if differential protein display by two-dimensional (2-D) gel electrophoresis and subsequent protein identification by matrix assisted laser desorption/ionization-time of flight (MALDI-TOF)-mass spectrometry could reveal proteins induced by cytokine stimulation that can be correlated to the transformation of normal @entity1 fetal lungs cells into a more myofibroblast like phenotype. After stimulation with @entity2533 ( @entity2533 ) several myofibroblast markers were found to be regulated. Especially cytoskeletal and cytoskeletal-associated proteins like actin isoforms and tropomyosin, proteins that are responsible for contraction as well as transportation of extra cellular matrix proteins, which are overproduced in the formation of @entity82 . These results indicate that @entity2533 , which is increased in a fibrotic process, participates in the transformation of fibroblasts to myofibroblasts.

A proteomic approach to mimic XXXX disease evolvement by an in vitro cell line.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity8173 :: ('3078', 'Gene') :: ['HFL-1'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis'] @entity565 :: ('MESH:D001249', 'Disease') :: ['asthma'] @entity2533 :: ('7040', 'Gene') :: ['TGF-beta', 'transforming growth factor-beta']
@entity82 :: (MESH:D005355,Disease) :: ['fibrosis']
