In the @entity1 retina, two types of catecholaminergic amacrine cells have been described. Although dopaminergic type 1 cells are well characterized, the physiology of type 2 cells is, so far, unknown. To target type 2 cells specifically, we used a transgenic @entity19 line that expresses green fluorescent protein under the control of the @entity669 hydroxylase promoter. Type 2 cells are GABAergic and have an extensive @entity2343 , which stratifies in the middle of the inner plexiform layer. Our data suggest that type 2 cells comprise two subpopulations with identical physiological properties: one has its somata located in the inner nuclear layer and the other in the ganglion cell layer. Immunostaining with bipolar cell markers suggested that type 2 cells receive excitatory inputs from type 3 OFF and type 5 ON bipolar cells. Consistently, patch-clamp recordings showed that type 2 cells are ON-OFF amacrine cells. Blocking excitatory inputs revealed that different rod and cone pathways are active under scotopic and mesopic light conditions. Blockade of inhibitory inputs led to membrane potential oscillations in type 2 cells, suggesting that GABAergic and glycinergic amacrine cells strongly influence type 2 cell signaling. Among the glycinergic amacrine cells, we identified the @entity53794 -immunoreactive amacrine cell as a likely candidate. Collectively, light responses of type 2 cells were remarkably uniform over a wide range of light intensities. These properties point toward a general function of type 2 cells that is maintained under scotopic and mesopic conditions.

Inputs underlying the ON-OFF light responses of type 2 wide-field amacrine cells in TH::GFP XXXX .

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity53794 :: ('216227(Tax:10090)', 'Gene') :: ['VGluT3'] @entity2343 :: ('MESH:D007635', 'Disease') :: ['dendritic arbor'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine']
@entity19 :: (10090,Species) :: ['mice']
