Chitin, the biopolymer of the @entity3954 , is the most abundant biopolymer on the planet after cellulose. However owing to its crystalline nature, its deacetylated derivative; chitosan is industrially more potent. This conversion on an enzymatic scale can be made using chitin deacetylase. The metagenomics library constructed from the soil exposed to chitin and chitosan yielded chitin modifying enzymes, one of them being chitin deacetylase (CDA) utilized for the present study. The gene was amplified and expressed using the pET 22b vector in @entity526 Rosetta cells. The effect of two additives; chitin and @entity653 on the CDA activity were studied. The inclusion of @entity653 in the medium improved the biomass by 50% from the initial value of 1.25g/l to 2.5g/l. The activity of CDA increased from 90 mol/min/ml to 343 mol/min/ml. The CDA activity reported in the present paper is the highest observed for any strain. The addition of @entity653 to the media not only helped improve the yield of the chitin deacetylase but also imparted value addition to the waste of the biofuel industry.

Expression studies of Bacillus licheniformis chitin deacetylase in XXXX Rosetta cells.

@entity526 :: ('562', 'Species') :: ['E. coli'] @entity653 :: ('MESH:D005990', 'Chemical') :: ['glycerol'] @entity3954 :: ('CHEBI:28009', 'Chemical') :: ['N-acetylglucosamine']
@entity526 :: (562,Species) :: ['E. coli']
