RATIONALE: @entity401 ( @entity401 ) are associated with oxidative stress. Plasma biomarkers that are directly linked to oxidative stress responses in this disease have not been identified. Stable oxidation products of @entity4149 residues in proteins may reflect the oxidative microenvironment in the lung or a systemic inflammatory state. OBJECTIVES: To determine if levels of protein @entity4149 oxidation are elevated in plasma of @entity1 with @entity3130 compared with an age- and sex-matched healthy control cohort. METHODS: Three @entity4149 oxidation products ( @entity42869 , @entity6837 , and @entity21614 ) were quantified by tandem mass spectrometry in cellular models, a @entity19 model of @entity130 induced @entity82 , and in plasma of healthy control subjects and @entity1 with @entity3130 (n   =   42 in each group). MEASUREMENTS AND MAIN RESULTS: Plasma levels of @entity42869 , @entity6837 , and @entity21614 were markedly elevated in @entity1 with @entity3130 compared with control subjects with receiver operating characteristic curves separating these groups of 0.872, 0.893, and 0.997, respectively. In a @entity19 model of @entity82 , levels of all three oxidative @entity4149 modifications were increased in plasma and lung tissue. Cellular models support a critical role for a @entity1446 peroxidase and enzymatic sources of reactive @entity26 species in the generation of these oxidized products. CONCLUSIONS: We demonstrate an increase in oxidized @entity4149 moieties within proteins in the circulating plasma of @entity1 with @entity3130 . These data support the potential for development of oxidative stress-related biomarkers in early diagnosis, prognostication, and/or in evaluating responsiveness to emerging therapies for @entity3130 .

Oxidative Modifications of Protein Tyrosyl Residues Are Increased in Plasma of XXXX Subjects with @entity401 .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity3130 :: ('MESH:C562470', 'Disease') :: ['ILD'] @entity6837 :: ('CHEBI:44454', 'Chemical') :: ['3-nitrotyrosine'] @entity82 :: ('MESH:D005355', 'Disease') :: ['fibrosis', 'lung fibrosis'] @entity19 :: ('10090', 'Species') :: ['mouse', 'murine'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity1446 :: ('MESH:D006418', 'Chemical') :: ['heme'] @entity21614 :: ('CHEBI:50607', 'Chemical') :: ["o,o'-dityrosine"] @entity401 :: ('MESH:D017563', 'Disease') :: ['Interstitial lung diseases', 'ILDs'] @entity4149 :: ('D014443', 'Chemical') :: ['tyrosine'] @entity130 :: ('MESH:D014947', 'Disease') :: ['injury-'] @entity42869 :: ('CHEBI:53678', 'Chemical') :: ['3-chlorotyrosine']
@entity1 :: (9606,Species) :: ['Human']
