To examine the role of protein kinase A (EC 2.7.1.37) isozymes in the @entity1968 -induced growth inhibition and neuronal differentiation, we investigated the changes of protein kinase A isozyme patterns in @entity1968 -treated SH-SY5Y @entity1 @entity521 cells. @entity1968 induced growth inhibition and neuronal differentiation of SH-SY5Y cells in a dose- and time-dependent manner. Neuronal differentiation was evidenced by extensive neurite outgrowth, decrease of @entity4180 oncoprotein, and increase of @entity13573 mRNA. Type II protein kinase A activity increased by 1.5-fold in differentiated SH-SY5Y cells by @entity1968 treatment. The increase of type II protein kinase A was due to the increase of @entity37500 have been known to play important role(s) in the growth inhibition and differentiation of @entity5 cells, we further investigated the role of the increased type II protein kinase A by overexpressing @entity37500 in SH-SY5Y cells. The growth of @entity37500 -overexpressing cells was slower than that of parental cells, being comparable to that of @entity1968 -treated cells. @entity1968 treatment further increased the @entity37500 level and further inhibited the growth of @entity37500 -overexpressing cells, showing strong correlation between the level of @entity37500 and growth inhibition. However, @entity37500 -overexpressing cells did not show any sign of neuronal differentiation and responded to @entity1968 in the same way as parental cells. These data suggest that protein kinase A participates in the @entity1968 -induced growth inhibition through the up-regulation of @entity37500 /type II protein kinase A.

Participation of type II protein kinase A in the @entity1968 -induced growth inhibition of SH-SY5Y @entity1 XXXX cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity37500 :: ('5577', 'Gene') :: ['RIIbeta', 'RIIbeta and Calpha subunits. Since type II protein kinase A and RIIbeta'] @entity13573 :: ('2596', 'Gene') :: ['GAP-43'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity521 :: ('MESH:D009447', 'Disease') :: ['neuroblastoma'] @entity4180 :: ('4613', 'Gene') :: ['N-Myc'] @entity1968 :: ('MESH:D014212', 'Chemical') :: ['retinoic acid', 'Retinoic acid']
@entity521 :: (MESH:D009447,Disease) :: ['neuroblastoma']
