Combinatorial diversity is highly restricted during formation of the pre-immune heavy chain repertoire of @entity1707 , raising the question of whether the same is true for the pre-immune light chain repertoire. Before addressing this question, we first used competitive PCR to show that kappa and lambda light chains in @entity1707 are equally expressed in mature B cells similar to the situation in @entity1 but alike that in other studied Ungulates. This justified efforts to examine the repertoire of both light chain types. These studies also revealed that lambda is preferentially expressed at sites of B cells lymphogenesis, perhaps because of the use of a surrogate light chain containing lambda5. Data are presented here on >100 VkappaJkappa-containing transcripts and approximately 180 genomic Vkappa genes to show that >90% of the pre-immune repertoire is generated from three subfamilies of @entity38175 genes and one of five Jkappa segments. The kappa locus contains >or=50 @entity38175 genes belonging to at least five subfamilies and an undetermined but perhaps equal number of @entity38175 genes. The porcine @entity38175 and @entity38175 genes share 87% sequence similarity with their @entity1 counterparts and Jkappa1 through Jkappa5 share sequence and organizational homology with those in @entity742 , @entity1641 , @entity1 and @entity19 . @entity1707 have a single Ckappa gene. These findings contrast with those from rodents and primates but are reminiscent of those on the pre-immune heavy chain repertoire of @entity1707 in that it is generated using a relatively restricted number of gene segments. These restricted pre-immune repertoires may reflect the minimal exposure of the fetus to maternal factors and environmental antigens. The significance for @entity1707 immunology of characterizing the pre-immune repertoire is discussed.

The pre-immune variable kappa repertoire of XXXX is selectively generated from certain subfamilies of Vkappa2 and one Jkappa gene.

@entity1 :: ('9606', 'Species') :: ['humans', 'human'] @entity1707 :: ('9823', 'Species') :: ['Swine', 'swine'] @entity1641 :: ('9796', 'Species') :: ['horse'] @entity742 :: ('9940', 'Species') :: ['sheep'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity38175 :: ('3519', 'Gene') :: ['IGKV1', 'IGKV2']
@entity1707 :: (9823,Species) :: ['swine']
