UNASSIGNED: Major surgery increases the risk for @entity166 due to the development of immunosuppression. CD56bright NK cells play a key role in the defense against @entity2348 through the release of Interferon (IFN) y upon stimulation with monocyte-derived Interleukin (IL) 12. We investigated whether invasive visceral surgery interferes with the @entity379 synthesis of @entity1 NK cells in response to @entity794 . In a prospective pilot study, peripheral blood mononuclear cells (PBMC) were isolated from 53 @entity1 before and 1 to 7 d after elective visceral surgery. The release of IL-12 and @entity379 from PBMC upon exposure to @entity794 in vitro was quantified. The expression of the IL-12 receptor b1 chain on the surface, the phosphorylation of signal transducer and activator of transcription (STAT) 4, and the synthesis of @entity379 on/in individual CD56bright NK cells were investigated using flow cytometry. The modulatory effect of IL-12 on the @entity794 -induced @entity379 production in CD56bright NK cells was analyzed. The @entity379 secretion from purified CD56bright NK cells was quantified after stimulation with IL-12 and IL-18. After surgery, CD56bright NK cells among total PBMC were impaired in the release of @entity379 for at least 5 d. Likewise, the IL-12-induced release of @entity379 from purified CD56bright NK cells was abolished. Upon stimulation with @entity794 , PBMC secreted less IL-12 but supplementation with recombinant IL-12 did not restore the capacity of CD56bright NK cells to produce @entity379 . CD56bright NK cells displayed reduced levels of the IL-12Rb1 chain whereas the phosphorylation of STAT4, the key transcription factor for the Ifng gene was not diminished. In summary, after invasive visceral surgery, CD56bright NK cells are impaired in @entity794 -induced @entity379 production and might contribute to the enhanced susceptibility to @entity1292 .

Invasive Surgery Impairs the Regulatory Function of @entity1 CD56bright Natural Killer Cells in Response to XXXX . Suppression of Interferon-y Synthesis.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity2348 :: ('MESH:D001424', 'Disease') :: ['bacterial infections'] @entity794 :: ('1280', 'Species') :: ['Staphylococcus aureus', 'S. aureus'] @entity379 :: ('3458', 'Gene') :: ['IFN-y'] @entity1292 :: ('MESH:D009894', 'Disease') :: ['opportunistic infections'] @entity166 :: ('MESH:D003141', 'Disease') :: ['infectious complications']
@entity794 :: (1280,Species) :: ['Staphylococcus aureus']
