UNASSIGNED: We have recently developed two hemi- @entity267 models (Bartok et al., Toxicol in Vitro 29, 72, 2015; Zorn-Kruppa et al. PLoS One 9, e114181, 2014), which allow the correct prediction of eye irritation potential of chemicals according to the United Nations globally harmonized system of classification and labeling of chemicals (UN GHS). Both models comprise a multilayered epithelium and a stroma with embedded keratocytes in a collagenous matrix. These two models were compared, using a set of fourteen test chemicals. Their effects after 10 and 60minutes (min) exposure were assessed from the quantification of cell viability using the @entity98 reduction assay. The first approach separately quantifies the damage inflicted to the epithelium and the stroma. The second approach quantifies the depth of injury by recording cell death as a function of depth. The classification obtained by the two models was compared to the Draize @entity95 eye test and an ex vivo model using @entity95 @entity267 (Jester et al. Toxicol in Vitro. 24, 597-604, 2010). With a 60min exposure, both of our models are able to clearly differentiate UN GHS Category 1 and UN GHS Category 2 test chemicals.

Assessment of the eye irritation potential of chemicals: A comparison study between two test methods based on human 3D hemi- XXXX models.

@entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity267 :: ('MESH:D065306', 'Disease') :: ['cornea'] @entity95 :: ('9986', 'Species') :: ['rabbit']
@entity267 :: (MESH:D065306,Disease) :: ['cornea']
