In our previous studies, we established a lymphogenous metastatic @entity915 @entity19 model using orthotopic implantation of @entity1 @entity2 cell lines. However, the lymphogenous metastatic potential of each cell line in our models does not reflect that of a @entity5 . In this study, we made orthotopic implanted models using primary cultured cells from surgically-resected @entity2 tissues. Tissues of 5 @entity1 with @entity1149 ( @entity1149 ) were applied to a primary culture method using a collagen gel coated flask. Suspensions of 2.0x10(4) @entity5 cells were injected into the left lung of @entity915 @entity19 . We could maintain primary culture cells from 2 (FM205 and FT821 cells) of 5 @entity2 , and made orthotopically implanted @entity915 @entity19 models. The size of both @entity5 in implanted sites of the lung increased with time. The FM205 cells microscopically were metastasized to the mediastinum by 4 weeks after implantation and macroscopically metastasized by 16 weeks. The FT821 cells were microscopically metastasized to the mediastinum by 4 weeks after implantation and macroscopically metastasized by 6 weeks. The lymphogenous metastatic potential of these primary culture cells was similar to that of clinical @entity5 . As the lymphogenous metastatic potential of this model reflects that of the clinical @entity5 , it is useful for elucidating the mechanism of lymphogenous metastasis and selecting anticancer drugs suitable for an individual @entity1 .

Establishment of @entity1 -like @entity915 @entity19 model by orthotopically implanting primary cultured cells from surgically-resected XXXX tissues.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'primary tumor', 'tumors'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung cancers', 'lung cancer'] @entity1149 :: ('MESH:D002289', 'Disease') :: ['NSCLC', 'non-small cell lung cancer'] @entity915 :: ('MESH:D053632', 'Disease') :: ['SCID']
@entity2 :: (MESH:D008175,Disease) :: ['lung cancer']
