UNASSIGNED: The transition period in dairy @entity291 is characterized by major changes in @entity413 and adipose tissue metabolism. The @entity58976 ( @entity58976 ) PPARy co-activator 1a ( @entity23400 ) axis might be related to the adiponectin ( @entity33605 ) system to orchestrate the regulation of these processes. We aimed to assess the mRNA abundance of the aforementioned components in ono visceral and one subcutaneous fat depot, together with the @entity33605 concentrations in serum of dairy @entity291 from late gestation to early lactation. In addition, the effect of 2 diets differing in energy density was tested. Twenty pluriparous German Holstein @entity291 were all kept on the same silage-based diet until d 42 antepartum. From then on until d 1 antepartum, 10 animals each were assigned to either high-concentrate (60:40 concentrate:roughage) or low-concentrate (30:70) diets. Both groups were further subdivided into a control and a @entity1670 group, the latter receiving 24 g/d @entity1670 from d -42 until d 24. From d 1 postpartum (p.p.) to d 24 p.p., the concentrate portion was increased from 30 to 50% for all @entity291 . Biopsies of subcutaneous (SCAT) and @entity1386 ( @entity1386 ) were taken at d -42, 1, 21, and 100 relative to parturition. Blood samples were drawn along with the biopsies as well as on d -21, -14, -7, -3, 1, 3, 7, 14, 21, 28, 35, 42, 63, 82, and 100 relative to calving. Quantification of target mRNA was done using quantitative PCR and serum @entity33605 concentration was measured via ELISA. The feeding regimen did not affect the variables examined. Serum @entity33605 concentrations decreased toward parturition, returned to precalving levels within 1 wk after parturition, and remained on a constant level until the end of the experiment. The mRNA abundance of @entity58976 , @entity23400 , @entity70196 , and the @entity33605 receptors 1 ( @entity70197 ) and 2 ( @entity40480 ) changed in SCAT and @entity1386 during the considered time period. Comparing SCAT and @entity1386 , the mRNA of @entity58976 , @entity70197 , and @entity40480 were more abundant in @entity1386 , whereas @entity23400 and @entity70196 were expressed more highly in SCAT. The protein abundance of @entity58976 tended to increase from d -42 to 21. At d 21 we detected more @entity23400 protein in the low-concentrate group as compared with the high-concentrate group. The correlations observed point to a link between these factors and might hint to a functional role of the variables in the regulation of @entity413 metabolism. This study substantiates the existence of the @entity58976 - @entity23400 -axis and indicates a functional relationship between @entity58976 and @entity70197 in @entity291 adipose tissue.

Longitudinal changes in adipose tissue of dairy @entity291 from late pregnancy to lactation. Part 2: The SIRT- XXXX axis and its relationship with the adiponectin system.

@entity33605 :: ('282865(Tax:9913)', 'Gene') :: ['ADIPOQ'] @entity1670 :: ('MESH:D009525', 'Chemical') :: ['nicotinic acid', 'niacin'] @entity1386 :: ('MESH:D018205', 'Disease') :: ['retroperitoneal adipose tissue', 'RPAT'] @entity58976 :: ('613629(Tax:9913)', 'Gene') :: ['Sirtuin-1', 'SIRT1'] @entity70197 :: ('407235(Tax:9913)', 'Gene') :: ['ADIPOR1'] @entity40480 :: ('407234(Tax:9913)', 'Gene') :: ['ADIPOR2'] @entity23400 :: ('338446(Tax:9913)', 'Gene') :: ['PPARGC1A'] @entity70196 :: ('520472(Tax:9913)', 'Gene') :: ['NAMPT'] @entity291 :: ('9913', 'Species') :: ['cows', 'bovine'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity23400 :: (338446(Tax:9913),Gene) :: ['PPARGC1A']
