Methylation of @entity5 suppressor genes has been implicated in @entity0 development. However, methylation profiles of different @entity1558 , subtypes of @entity189 in particular, have not been examined in detail. In this study, we use methylation-specific PCR (MSP) to generate gene methylation profiles of different @entity1558 and to test the clinical utility of such profiles. We examined the methylation status of three genes, RARbeta2, @entity15893 , and @entity17946 , on 102 samples of breast tissue, from benign (n = 36), to in situ @entity189 (n = 21), to @entity189 (n = 45). We found that almost all cases of @entity189 (96%) contained at least one methylated gene from our panel, whereas gene methylation was less common among benign lesions (42%) and in situ @entity189 (76%). Of the three genes, @entity17946 methylation was most specific for @entity5 because only 1 of 35 benign cases was methylated at this gene (1 case was not informative). The major histologic subtypes of @entity189 show similar methylation profiles in the genes examined. We next performed MSP analysis on archival breast fine-needle aspiration (FNA) biopsy samples and corresponding surgical biopsy specimens and found a high concordance between the two types of specimens. We then analyzed 17 breast FNA biopsy samples with an indeterminate diagnosis. In this setting, MSP had a high specificity (100%) and modest sensitivity (67%) for identifying @entity5 .

Methylation profiling of benign and malignant XXXX and its application to cytopathology.

@entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancer'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'malignancy'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinoma', 'invasive carcinoma'] @entity15893 :: ('11186', 'Gene') :: ['RASSF1A'] @entity1558 :: ('MESH:D001941', 'Disease') :: ['breast lesions'] @entity17946 :: ('894', 'Gene') :: ['cyclin D2']
@entity1558 :: (MESH:D001941,Disease) :: ['breast lesions']
