INTRODUCTION: @entity189 ( @entity189 ) is a common @entity135 . The @entity69843 gene is related to development of @entity5 . The aim of this study is to explore the promoter region of the @entity69843 gene. MATERIAL AND METHODS: The bioinformatic technique was applied to predict its promoter region and construct the gene promoter region luciferase for the gene vector and transfection of the @entity1 embryonic kidney epithelial 293T cell line, @entity1 @entity189 CNE2 cell line and immortalized nasopharyngeal epithelial NP69 cell line. The recombinant plasmid @entity10169 -en283, @entity10169 -en281, @entity10169 -en571, empty plasmid @entity10169 -control, negative control @entity10169 -enhance and internal control of marine intestine luciferase expression vector pRL-SV40 were transfected into NP69 cells, 293T cells and CNE2 cells. Dual luciferase activity detection showed luciferase luminescence values and marine intestine luciferase luminescence values. Relative luciferase activity (RLA) in each cell was calculated. RESULTS: We observed strong promoter activity of plasmid @entity10169 -en283, @entity10169 -en281 and @entity10169 -en571 in NP69, 293T and CNE2 cells compared with the negative control @entity10169 -enhance plasmid. Among them, @entity10169 -en281 showed the strongest promoter activity, and these three kinds of recombinant plasmids showed stronger promoter activity in 293T cells than in CNE2 cells. CONCLUSIONS: The @entity10169 -en281 plasmid showed stronger promoter activity than @entity10169 -en571 in the three cells, indicating that -11048 bp to -653 bp might be the core promoter region.

Identification and analysis of the promoter region of the XXXX gene.

@entity1 :: ('9606', 'Species') :: ['human'] @entity135 :: ('MESH:D006258', 'Disease') :: ['malignant tumor of the head and neck'] @entity10169 :: ('6391', 'Gene') :: ['pGL3'] @entity5 :: ('MESH:D009369', 'Disease') :: ['nasopharyngeal cancer'] @entity189 :: ('MESH:D002277', 'Disease') :: ['Nasopharyngeal carcinoma', 'nasopharyngeal carcinoma', 'NPC'] @entity69843 :: ('474171', 'Gene') :: ['STGC3']
@entity69843 :: (474171,Gene) :: ['STGC3']
