BACKGROUND AND AIM: @entity14 ( @entity14 ) is a complication of @entity31 ( @entity31 ). Although stool occult blood and colonoscopy are used to detect @entity14 in @entity31 , these methods have drawbacks, in that @entity548 is associated with @entity31 and the underlying mucosa is irregular, making it difficult to detect @entity1141 . Telomerase and its catalytic subunit, telomerase reverse transcriptase ( @entity2215 ), are specifically expressed in @entity5 , making them candidate markers for the early detection of @entity5 . We previously reported that assays of telomerase in pancreatic juice may be useful for the early detection of @entity788 . The aims of our study were to determine whether assays for telomerase and @entity2215 may be useful in the diagnosis of @entity14 developed in @entity31 @entity1 . METHODS: Luminal washings and biopsies were collected during colonoscopy in 66 @entity1 ; 34 with @entity14 , 21 with @entity31 , and 11 controls. Telomerase activity was detected by telomeric repeat amplification protocol (TRAP) and @entity2215 mRNA was assayed by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Telomerase activity was detected in biopsies from 33 of 34 (97%) @entity14 @entity1 , 14 of 21 (67%) @entity31 @entity1 , and three of 11 (27%) normal controls. Expression of @entity2215 mRNA was observed in biopsies from 32 of 34 (94%) @entity14 @entity1 , 12 of 21 (57%) @entity31 @entity1 , and five of 11 (45%) controls. In analyses of washing fluid, 21 of 34 (62%) of @entity14 @entity1 were positive for telomerase, but all @entity31 @entity1 and controls were negative. The sensitivity of telomerase for @entity14 was 97% in tissues and 62% in washing fluid. The specificity of telomerase in washing fluid was 100%, whereas the specificity of telomerase or @entity2215 mRNA in tissues was 47% each. CONCLUSIONS: For the detection of @entity14 arising in @entity31 , telomerase and @entity2215 in colonic tissue showed high sensitivity, and telomerase in colonoscopic luminal washings revealed a high specificity. These might be candidate markers in clinical decision making for the diagnosis of @entity14 from @entity31 .

Clinical usefulness of telomerase for the detection of colon cancer in @entity31 XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity788 :: ('MESH:D010190', 'Disease') :: ['pancreatic cancer'] @entity548 :: ('MESH:D006470', 'Disease') :: ['bleeding'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancers', 'cancer'] @entity31 :: ('MESH:D003093', 'Disease') :: ['ulcerative colitis', 'UC'] @entity14 :: ('MESH:D015179', 'Disease') :: ['Colorectal carcinoma', 'CRC'] @entity2215 :: ('7015', 'Gene') :: ['TERT', 'hTERT'] @entity1141 :: ('MESH:C535662', 'Disease') :: ['dysplasia']
@entity1 :: (9606,Species) :: ['patients']
