Upon recognition of microbe-associated molecular patterns (MAMPs) such as the bacterial flagellin (or the derived peptide flg22) by pattern-recognition receptors (PRRs) such as the @entity35038 ( @entity35038 ), plants activate the pattern-triggered immunity (PTI) response. The L-type lectin receptor kinase-VI.2 (LecRK-VI.2) is a positive regulator of @entity958 PTI. @entity462 -rich receptor-like kinases (CRKs) possess two copies of the C-X8-C-X2-C (DUF26) motif in their extracellular domains and are thought to be involved in plant stress resistance, but data about @entity24417 functions are scarce. Here, we show that @entity958 overexpressing the LecRK-VI.2-responsive @entity24420 , @entity69704 , and @entity69705 demonstrated an enhanced PTI response and were resistant to virulent bacteria @entity14348 . Notably, the flg22-triggered oxidative burst was primed in @entity24420 , @entity69704 , and @entity69705 transgenics and up-regulation of the PTI-responsive gene @entity69706 ( @entity69706 ) was potentiated upon flg22 treatment in @entity24420 and @entity69704 overexpression lines or constitutively increased by @entity69705 overexpression. PTI-mediated callose deposition was not affected by overexpression of @entity24420 and @entity69704 , while @entity69705 overexpression lines demonstrated constitutive accumulation of callose. In addition, Pst @entity14348 -mediated stomatal reopening was blocked in @entity24420 and @entity69705 overexpression lines, while overexpression of @entity69704 induced constitutive stomatal closure suggesting a strengthening of stomatal immunity. Finally, bimolecular fluorescence complementation and co-immunoprecipitation analyses in @entity958 protoplasts suggested that the plasma membrane localized @entity24420 , @entity69704 , and @entity69705 associate with the PRR @entity35038 . Association with @entity35038 and the observation that overexpression of @entity24420 , @entity69704 , and @entity69705 boosts specific PTI outputs and resistance to bacteria suggest a role for these CRKs in @entity958 innate immunity.

Enhanced @entity958 pattern-triggered immunity by overexpression of XXXX -rich receptor-like kinases.

@entity462 :: ('MESH:D003545', 'Chemical') :: ['Cysteine'] @entity35038 :: ('834676(Tax:3702)', 'Gene') :: ['FLS2', 'FLAGELLIN SENSING2'] @entity958 :: ('3702', 'Species') :: ['Arabidopsis thaliana', 'Arabidopsis'] @entity69706 :: ('816436(Tax:3702)', 'Gene') :: ['FLG22-INDUCED RECEPTOR-LIKE 1', 'FRK1'] @entity14348 :: ('223283', 'Species') :: ['Pseudomonas syringae pv. tomato DC3000', 'DC3000'] @entity69705 :: ('825779(Tax:3702)', 'Gene') :: ['CRK36'] @entity69704 :: ('828413(Tax:3702)', 'Gene') :: ['CRK6'] @entity24417 :: ('828087(Tax:3702)', 'Gene') :: ['CRK'] @entity24420 :: ('823729(Tax:3702)', 'Gene') :: ['CRK4']
@entity462 :: (MESH:D003545,Chemical) :: ['cysteine']
