@entity981  ( @entity981 ), which involves the blood and bone marrow, is the most common type of @entity5 in @entity1 younger than 5  years of age. Previous studies have investigated the effects of centipedegrass extract  (CGE), which is mainly composed of maysin and its derivatives, and have demonstrated that it has various biological activities, including antioxidant and anti  -inflammatory activities, pancreatic lipase inhibitory activity, anti-adipogenic activity and insecticidal activity. To the best of our knowledge, this study is the first to investigate the anticancer effects of CGE in @entity981 cell lines and to elucidate the mechanisms underlying these effects. Cell viability was measured by thiazolyl blue tetrazolium blue  ( @entity98 ) assay. Apoptosis, cell cycle progression and mitochondrial membrane potential  (     m) were determined by flow cytometry. The effects of CGE on the @entity4357 3  -kinase  ( @entity10412 )/ @entity584 pathway and mitogen  -activated protein kinases  (MAPKs) were assessed by immunoblotting. @entity10412 , MAPK and caspase inhibitors were used to further confirm the molecular mechanisms involved. Our results clearly demonstrated that the proliferation of the @entity981 cells was significantly inhibited by CGE in a dose  -dependent manner. Apoptosis was accompanied by the induction of significant G1  cell cycle arrest. The resulting alteration of the      m increased the activity of @entity31373 . The induction of apoptosis was enhanced by the combined treatment of CGE with a @entity10412 inhibitor or an @entity3963 ( @entity3963 ) inhibitor, whereas the CGE  -induced apoptosis was inhibited in the presence of caspase inhibitors, such as z  -VAD  -fmk and z  -IETD  -fmk. Furthermore, CGE inhibited @entity10412 activity by decreasing the levels of phosphorylated (p  -) @entity584 , p  -BAD, and @entity588 together with the levels of MAPKs, including p  - @entity3963 and p  -JNK, but demonstrated no effects on p38  MAPK. Thus, our data suggest that CGE may be a novel natural compound with potential for use as an antitumor agent in @entity981 .

Centipedegrass extract induces apoptosis through the activation of caspases and the downregulation of XXXX / @entity584 and MAPK phosphorylation in leukemia cells.

@entity1 :: ('9606', 'Species') :: ['children'] @entity981 :: ('MESH:D054198', 'Disease') :: ['Acute lymphoblastic leukemia', 'ALL'] @entity31373 :: ('836;840', 'Gene') :: ['caspase  -3/7'] @entity588 :: ('596', 'Gene') :: ['Bcl  -2'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity3963 :: ('5594', 'Gene') :: ['extracellular signal-regulated kinase', 'ERK'] @entity584 :: ('207', 'Gene') :: ['Akt'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity10412 :: ('5293', 'Gene') :: ['PI3K'] @entity4357 :: ('MESH:D010716', 'Chemical') :: ['phosphatidylinositol']
@entity10412 :: (5293,Gene) :: ['PI3K']
