RATIONALE: Allogeneic bone marrow-derived mesenchymal stem cells (MSCs) and cardiosphere-derived cells (CDCs) have each entered clinical trials but a direct comparison of these cell types has not been performed in a large animal model of hibernating @entity1562 . OBJECTIVE: Using completely blinded methodology, compare the efficacy of global intracoronary allogeneic MSCs (icMSCs,~35x10(6)) and CDCs (icCDCs,~35x10(6)) vs. vehicle in @entity205 -immunosuppressed @entity1707 with a chronic @entity3980 (n=26). METHODS AND RESULTS: Studies began 3-months after instrumentation when wall-thickening (%WT) was reduced (LAD%WT 38  11% (mean  SD) vs. 83  26% in remote, p<0.01) and similar among groups. Four-weeks after treatment, LAD%WT increased similarly following icCDCs and icMSCs, while it remained depressed in vehicle-treated controls (icMSCs: 51  13%; icCDCs: 51  17%; vehicle: 34  3%, treatments p<0.05 vs. vehicle). There was no change in myocardial perfusion. Both icMSCs and icCDCs increased LAD myocyte nuclear density (icMSCs: 1601  279 nuclei/mm2, icCDCs: 1569  294 nuclei/mm2, vehicle: 973  181 nuclei/mm2, treatments p<0.05 vs. vehicle) and reduced myocyte diameter (icMSCs: 16.4  1.5   m, icCDCs: 16.8  1.2   m, vehicle: 20.2  3.7   m, treatments p<0.05 vs. vehicle) to the same extent. Similar changes in myocyte nuclear density and diameter were observed in the remote region of cell-treated animals. Cell fate analysis using Y-FISH demonstrated rare cells from sex-mismatched donors. CONCLUSIONS: Allogeneic icMSCs and icCDCs exhibit comparable therapeutic efficacy in a large animal model of hibernating @entity1562 . Both cell types produced equivalent increases in regional function and stimulated myocyte regeneration in @entity63 . The activation of endogenous myocyte proliferation and regression of myocyte cellular hypertrophy support a common mechanism of cardiac repair.

Comparative Efficacy of Intracoronary Allogeneic Mesenchymal Stem Cells and Cardiosphere-Derived Cells in XXXX with Hibernating Myocardium.

@entity1707 :: ('9823', 'Species') :: ['swine'] @entity63 :: ('MESH:D007511', 'Disease') :: ['ischemic and remote myocardium'] @entity205 :: ('MESH:D016572', 'Chemical') :: ['cyclosporine'] @entity1562 :: ('MESH:D017682', 'Disease') :: ['myocardium'] @entity3980 :: ('MESH:C535887', 'Disease') :: ['LAD stenosis']
@entity1707 :: (9823,Species) :: ['Swine']
