OBJECTIVE: The effect of miR-449 and @entity7762 on the growth, cell cycle and target gene expressions of @entity43 cell line SKOV3 and SKOV3-ipl was discussed. METHOD: Real-time quantitative reverse transcription PCR was employed to detect the expressions of @entity33670 /b and @entity27579 , c in SKOV3 and SKOV3-ipl cells. The two miRNAs were successfully expressed in SKOV3-ipl cells by transfection. The variations in cell growth rate and cell cycle were determined by MTS assay and flow cytometry, respectively. The expressions of cell cycle-related proteins were detected by Western Blot. RESULTS: @entity33671 and @entity21066 induced the decline of the adhesiveness of SKOV3-ipl cells by 20%-30%. The number of cells arrested in G1-phase increased and the number of cells arrested in S-phase decreased significantly. The cell cycle-related proteins @entity14107 and CDC254 were downregulated. @entity33671 caused the expression of @entity14107 and @entity10837 to decrease. After the co-transfection with @entity33671 and @entity21066 , the relevant proteins were downregulated more significantly. The expressions of @entity14107 , @entity10837 and @entity7596 were decreased significantly. CONCLUSION: @entity33671 and @entity21066 can induce cell cycle arrest in SKOV3-ipl cells and the downregulation of @entity14107 , @entity10837 and @entity7596 .

Downregulation of cell cycle-related proteins in XXXX line and cell cycle arrest induced by microRNA.

@entity43 :: ('MESH:D010051', 'Disease') :: ['ovarian cancer'] @entity27579 :: ('407041', 'Gene') :: ['miR-34b'] @entity21066 :: ('407042', 'Gene') :: ['miR-34c'] @entity14107 :: ('1021', 'Gene') :: ['CDK6'] @entity7762 :: ('407040', 'Gene') :: ['miR-34'] @entity33670 :: ('554213', 'Gene') :: ['miR-449a'] @entity33671 :: ('693123', 'Gene') :: ['miR-449b'] @entity7596 :: ('890', 'Gene') :: ['cyclin A'] @entity10837 :: ('993', 'Gene') :: ['CDC25A']
@entity43 :: (MESH:D010051,Disease) :: ['ovarian cancer']
