The elevated exogenous- @entity1853 dependency of @entity5 for growth has been observed in all major @entity5 cell types. We have previously cloned a methioninase ( @entity25535 ) from @entity15250 to deplete @entity1853 . Growth inhibition followed by apoptotic cell death was induced by treatment of @entity5 cells with @entity25535 in vitro. A single i.p. injection of 300 units of @entity25535 can lower the serum @entity1853 level in the @entity19 from 70 microM to less than 1 microM within 2 h and maintain this depleted level for 8 h. Repeated dosing of @entity25535 of @entity5 -bearing @entity19 could be administered without acute immune-hypersensitivity. @entity25535 treatment demonstrated growth inhibitory activity against @entity1 @entity5 in @entity19 , including those which were multiple drug-resistant. No body weight loss or hematotoxicity, except a slight @entity417 , was found throughout the therapy. The combined treatment of the @entity2 with a fixed @entity25535 dose and increasing doses of @entity1487 ( @entity1487 ) resulted in a dose-dependent enhanced antitumor efficacy for survival as well as @entity5 growth inhibition. Thus, @entity1853 depletion by @entity25535 potentiates the antitumor efficacy of @entity1487 . The data presented in this report thus indicate that @entity25535 is active alone, is synergistic in combination with @entity1487 , and has negligible @entity137 suggesting a novel clinical approach for effective @entity5 therapy.

Anticancer efficacy in vivo and in vitro, synergy with XXXX , and safety of recombinant methioninase.

@entity1 :: ('9606', 'Species') :: ['human'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity25535 :: ('29252(Tax:10116)', 'Gene') :: ['rMETase'] @entity417 :: ('MESH:D000740', 'Disease') :: ['anemia'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'tumors'] @entity19 :: ('10090', 'Species') :: ['mice', 'nude mice'] @entity2 :: ('MESH:D008175', 'Disease') :: ['Lewis lung carcinoma'] @entity15250 :: ('303', 'Species') :: ['Pseudomonas putida'] @entity1853 :: ('MESH:D008715', 'Chemical') :: ['methionine'] @entity1487 :: ('MESH:D005472', 'Chemical') :: ['5-FU', '5-fluorouracil']
@entity1487 :: (MESH:D005472,Chemical) :: ['5-fluorouracil']
