UNASSIGNED: @entity1 norovirus (HuNoV) is the leading cause of @entity6766 with an increasing number of outbreaks associated with leafy greens. Because HuNoV cannot be routinely cultured, culturable @entity21063 ), @entity19 norovirus (MNV), porcine sapovirus (SaV) and Tulane virus (TV) have been used as surrogates. These viruses are generated in different cell lines as infected cell lysates which may differentially affect their stability. Our objective was to uniformly compare the survival of these viruses on postharvest lettuce, while evaluating the effect of cell lysates on their survival. Viruses were semi-purified from cell lysates by ultrafiltration or ultracentrifugation followed by re-suspension in sterile water. Virus survival was examined before and after semi-purifications: in suspension at @entity1054 ( @entity1054 ) until day 28 and on lettuce leaves stored at @entity1054 for 3 days, or 4   C for 7 and 14 days. In suspension, both methods significantly enhanced the survival of all viruses. On lettuce, the survival of MNV in cell lysates was similar to that in water, under all storage conditions. In contrast, the survival of @entity21063 , SaV and TV was differentially enhanced, under different storage conditions, by removing cell lysates. Following semi-purification viruses showed similar persistence to each other on lettuce stored under all conditions, with the exception of ultracentrifugation-purified @entity21063 which showed higher inactivation rate than MNV at 4    C for 14 days. In conclusion, the presence of cell lysates in viral suspensions underestimated the survivability of these surrogate viruses while viral semi-purifications revealed a similar survivability on postharvest lettuce leaves.

@entity21063 , XXXX Norovirus, Porcine Sapovirus and Tulane Virus Survival on Postharvest Lettuce.

@entity1 :: ('9606', 'Species') :: ['Human'] @entity21063 :: ('11978', 'Species') :: ['FCV', 'feline calicivirus (FCV'] @entity6766 :: ('MESH:D005517', 'Disease') :: ['foodborne illnesses'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity1054 :: ('MESH:D000377', 'Disease') :: ['RT', 'room temperature']
@entity19 :: (10090,Species) :: ['Murine']
