Transgenic @entity286 ( @entity286 .) plants ubiquitously accumulating a single-chain variable-fragment (scFv) antibody against @entity2271 ( @entity2272 ) to high concentrations in the endoplasmic reticulum (RA plants) show a wilty phenotype. High stomatal conductance and loss of @entity515 and light dependence of stomatal conductance are typical features of these plants. @entity2272 was applied to these plants either via the petioles or by daily spraying over several weeks in order to normalise the phenotype. During the long-term experiments, scFv protein concentrations, total and (calculated) free @entity2272 contents, and stomatal conductance and its dependence on @entity515 concentration and light intensity were monitored. The wilty phenotype of transgenic plants could not be normalised by short-term treatment with @entity2272 via the petioles. Only a daily long-term treatment during plant development normalised the physiological behaviour completely. Scanning electron microscopy of stomata showed morphological changes in RA plants compared with wild-type plants that, for structural reasons, prevented regular stomatal movements. After long-term treatment with @entity2272 this defect could be completely eliminated. Guard-cell-specific expression of the anti- @entity2272 scFv did not cause any changes in physiological behaviour compared to the wild type. In addition, mesophyll-specific expression starting in leaves that were already fully differentiated resulted in normal phenotypes, too. We conclude that changes in distribution and availability of @entity2272 in the cells of developing leaves of RA plants cause the development of structural features in stomata that prevent normal function.

Prevention of stomatal closure by immunomodulation of endogenous XXXX and its reversion by @entity2271 treatment: physiological behaviour and morphological features of @entity286 stomata.

@entity2272 :: ('CHEBI:2365', 'Chemical') :: ['ABA'] @entity286 :: ('4097', 'Species') :: ['Nicotiana tabacum L', 'tobacco'] @entity2271 :: ('MESH:D000040', 'Chemical') :: ['abscisic acid'] @entity515 :: ('CHEBI:29334', 'Chemical') :: ['CO(2)']
@entity2271 :: (MESH:D000040,Chemical) :: ['abscisic acid']
