OBJECTIVE: To characterize chondrocytes from naturally occurring @entity6995 ( @entity6995 ) lesions of the humeral head of @entity235 . SAMPLE POPULATION: 15 cartilage specimens from 13 client-owned @entity235 with humeral head @entity6995 and 10 specimens from the humeral head of healthy @entity235 (controls). PROCEDURE: Chondrocytes were isolated and cultured in a 3-dimensional system. On days 7, 10, 15, 20, and 25, glycosaminoglycan and @entity720 content and cytologic characteristics were evaluated. Expression of collagen types I, II, and X was assessed by use of immunohistochemistry. RESULTS: Chondrocytes from @entity6995 lesions were less viable, compared with control chondrocytes. Glycosaminoglycan content in the @entity6995 group was significantly less than in the control group on all days except day 20. @entity720 content was also significantly less in the @entity6995 group on days 10, 20, and 25. Expression of collagen type II was significantly less in the @entity6995 group, compared with the control group on all days, whereas expression of collagen type I was significantly greater in the @entity6995 group on days 20 and 25. Expression of collagen type X was significantly less in the @entity6995 group on all days except day 25. CONCLUSIONS AND CLINICAL RELEVANCE: Chondrocytes from naturally occurring @entity6995 lesions of the humeral head of @entity235 cultured in a 3-dimensional system were less viable and less capable of producing appropriate extracellular matrix molecules than chondrocytes from unaffected @entity235 . Alterations in the synthetic capabilities of chondrocytes from @entity6995 -affected cartilage may be a cause or an effect of the disease process.

In vitro characterization of chondrocytes isolated from naturally occurring osteochondrosis lesions of the humeral head of XXXX .

@entity235 :: ('9615', 'Species') :: ['dogs'] @entity6995 :: ('MESH:D055034', 'Disease') :: ['OC', 'osteochondrosis'] @entity720 :: ('CHEBI:18095', 'Chemical') :: ['Hydroxyproline', 'hydroxyproline']
@entity235 :: (9615,Species) :: ['dogs']
