We earlier demonstrated the immunoprophylactic efficacy of recombinant heavy chain @entity68494 (Bm-Myo) of @entity2757 ( @entity2757 ) in rodent models. In the current study, further attempts have been made to improve this efficacy by employing alternate approaches such as homologous DNA (pcD-Myo) and heterologous DNA/protein prime boost (pcD-Myo+Bm-Myo) in BALB/c @entity19 model. The gene bm-myo was cloned in a @entity1 expression vector pcDNA 3.1(+) and protein expression was confirmed in @entity1 Vero cell line. A significant degree of protection (79.2%  2.32) against L3 challenge in pcD-Myo+Bm-Myo immunized group was observed which was much higher than that exerted by Bm-Myo (66.6%  2.23) and pcD-Myo (41.6%  2.45). In the heterologous immunized group, the percentage of peritoneal leukocytes such as macrophages, neutrophils, B cells and T cells marginally increased and their population augmented further significantly following L3 challenge. pcD-Myo+Bm-Myo immunization elicited robust cellular and humoral immune responses as compared to pcD-Myo and Bm-Myo groups as evidenced by an increased accumulation of CD4+, CD8+ T cells and CD19+ B cells in the @entity19 spleen and activation of peritoneal macrophages. Though immunized animals produced antigen-specific IgG antibodies and isotypes, sera of @entity19 receiving pcD-Myo+Bm-Myo or Bm-Myo developed much higher antibody levels than other groups and there was profound antibody-dependent cellular adhesion and @entity137 (ADCC) to @entity2757 infective larvae (L3). pcD-Myo+Bm-Myo as well as Bm-Myo @entity19 generated a mixed T helper cell phenotype as evidenced by the production of both pro-inflammatory ( @entity2047 , IFN-y) and anti-inflammatory ( @entity58 , @entity1618 ) cytokines. @entity19 receiving pcD-Myo on contrary displayed a polarized pro-inflammatory immune response. The findings suggest that the priming of animals with DNA followed by protein booster generates heightened and mixed pro- and anti-inflammatory immune responses that are capable of providing high degree of protection against filarial larval invasion.

Immunogenicity and Protective Efficacy of XXXX Heavy Chain @entity68494 as Homologous DNA, Protein and Heterologous DNA/Protein Prime Boost Vaccine in Rodent Model.

@entity68494 :: ('6099114(Tax:6279)', 'Gene') :: ['myosin'] @entity1 :: ('9606', 'Species') :: ['mammalian'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity2757 :: ('6279', 'Species') :: ['Brugia malayi', 'B. malayi'] @entity2047 :: ('16183(Tax:10090)', 'Gene') :: ['IL-2'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'Mice'] @entity1618 :: ('16153(Tax:10090)', 'Gene') :: ['IL-10'] @entity58 :: ('16189(Tax:10090)', 'Gene') :: ['IL-4']
@entity2757 :: (6279,Species) :: ['Brugia malayi']
