BACKGROUND: The mechanism by which a low dose of @entity1499 ( @entity1500 ) works to treat @entity1569 is not clear. The overexpression of cell adhesion molecules on dermal vessels is important in the pathogenesis of @entity1569 and is probably induced by upregulation of @entity5 @entity39 factor ( @entity310 . OBJECTIVES: To determine the effects of @entity1500 at concentrations comparable with in vivo levels after the administration of low-dose @entity1500 to @entity1 umbilical vein endothelial cells (HUVEC) on the growth and expression of @entity2071 ( @entity2071 ) and @entity4725 ( @entity4725 ). METHODS: Cell proliferation assay, immunostaining, immunoblotting, cell enzyme-linked immunosorbent assay and reverse transcriptase-polymerase chain reaction (RT-PCR) were performed to examine the effects of @entity1500 on HUVEC. RESULTS: @entity1500 inhibited the proliferation of HUVEC at 10-7 mol @entity7373 mol L-1 without showing cytotoxic effects. It also inhibited @entity310 -induced @entity2071 and @entity4725 expression by HUVEC at 10-6 mol L-1. The inhibitory effect of @entity1500 was more pronounced on @entity2071 expression than on @entity4725 expression. RT-PCR analysis revealed that @entity310 -induced @entity2071 gene expression was strongly downregulated by @entity1500 . CONCLUSIONS: Low-dose @entity1500 may act on @entity1569 by suppressing the @entity310 -induced expression of @entity2071 and @entity4725 by vascular endothelial cells. Inhibition of neovascularization may be another mechanism of action of @entity1500 .

XXXX inhibits proliferation and regulation of the expression of @entity2071 and @entity4725 by cultured @entity1 umbilical vein endothelial cells.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1569 :: ('MESH:D011565', 'Disease') :: ['psoriasis'] @entity4725 :: ('7412', 'Gene') :: ['vascular cell adhesion molecule-1', 'VCAM-1'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumour'] @entity310 :: ('7124', 'Gene') :: ['TNF)-alpha', 'TNF-alpha'] @entity7373 :: ('3897', 'Gene') :: ['L-1 and 10-6'] @entity1500 :: ('CHEBI:44185', 'Chemical') :: ['MTX'] @entity2071 :: ('3383', 'Gene') :: ['ICAM-1', 'intercellular adhesion molecule-1'] @entity1499 :: ('MESH:D008727', 'Chemical') :: ['methotrexate']
@entity1499 :: (MESH:D008727,Chemical) :: ['Methotrexate']
