The present work reports on testicular hormone-sensitive lipase ( @entity58351 ), the biological significance of which has been documented in male fertility. The @entity58351 protein levels and enzymatic activity were measured, respectively, by densitometry of immunoreactive bands in Western blots, performed with antibodies against recombinant @entity35 @entity58351 , and by spectrophotometry in seminiferous tubules (STf) and interstitial tissue ( @entity54304 ) enriched fractions generated from neonatal, pubertal, and adult @entity840 testes. In addition, @entity58351 was studied in subcellular fractions obtained from STf isolated from adult testes and in epididymal spermatozoa (Spz). A 104-kDa @entity58351 protein was detected in STf and @entity54304 , the expression and activity of which increased with testicular development. Three immunoreactive bands of 104, 110, and 120 kDa were detected in the lysosomal subfraction, and two bands of 104 and 120 kDa were detected in Spz. The @entity58351 activity was positively correlated with free (FC) and esterified (EC) @entity165 ratios in STf and @entity54304 , but not with @entity412 (TG) levels, during testicular development. Immunolabeling localized @entity58351 to elongated spermatids and Sertoli cells, where its distribution was stage-dependent, and within the cells lining the excurrent ducts of the testis. The findings of the 104- and 120-kDa @entity58351 immunoreactive bands and of @entity58351 activity in Spz as well, as the detection of the 104-, 110-, and 120-kDa immunoreactive bands in lysosomes, suggest that part of @entity58351 may originate from germ cells and be imported in Sertoli cells. The @entity58351 protein levels and enzymatic activity in @entity54304 and STf were positively correlated with serum @entity251 levels during development. To the best of our knowledge, this study is the first to contribute insights regarding the impact of @entity58351 on FC:EC @entity165 ratios and TG levels in the interstitial tissue and tubules in relation to serum @entity251 levels during postnatal development, and regarding the immunolocalization of the enzyme in regions of the male gamete consistent with spermatozoa-oocyte interaction.

Expression, activity, and subcellular localization of testicular hormone-sensitive lipase during postnatal development in the XXXX .

@entity54304 :: ('25563(Tax:10116)', 'Gene') :: ['ITf'] @entity251 :: ('MESH:D013739', 'Chemical') :: ['testosterone'] @entity58351 :: ('25330(Tax:10116)', 'Gene') :: ['HSL'] @entity840 :: ('10141', 'Species') :: ['guinea pig'] @entity412 :: ('CHEBI:17855', 'Chemical') :: ['triglyceride'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity165 :: ('MESH:D002784', 'Chemical') :: ['cholesterol']
@entity840 :: (10141,Species) :: ['guinea pig']
