@entity1426 metabolism leads to the generation of biologically active metabolites that regulate cell growth and proliferation, as well as survival and apoptosis. We have demonstrated previously that @entity10327 (LOX) regulates the growth and survival of a number of @entity5 cells. In this study, we show that overexpression of platelet-type 12-LOX in @entity263 @entity11620 cells or epithelial @entity5 A431 cells significantly extended their survival and delayed apoptosis when cultured under serum-free conditions. These effects were shown to be a result of enhanced surface integrin expression, resulting in a more spread morphology of the cells in culture. @entity11620 cells transfected with 12-LOX displayed increased alpha(v)beta(3) and alpha(v)beta(5) integrin expression, whereas other integrins were unaltered. Transfected A431 cells did not express alpha(v)beta(3); however, alpha(v)beta(5) integrin expression was increased. Treatment of both transfected cell lines with monoclonal antibody to alpha(v)beta(5) (and in the case of @entity11620 cells, anti-alpha(v)beta(3)) resulted in significant apoptosis. In addition, treatment with 100 nM @entity11903 , the end product of platelet-type 12-LOX, but not other @entity11117 , enhanced the survival of wild-type @entity11620 and A431 cells and resulted in increased expression of alpha(v)beta(5). Furthermore, @entity19988 or @entity45658 , specific 12-LOX inhibitors, significantly decreased alpha(v)beta(5)-mediated adhesion and survival in 12-LOX-overexpressing cells. The results show that 12-LOX regulates cell survival and apoptosis by affecting the expression and localization of the vitronectin receptors, alpha(v)beta(3) and alpha(v)beta(5), in two @entity5 cell lines.

Overexpression of @entity10327 promotes XXXX cell survival by enhancing alpha(v)beta(3) and alpha(v)beta(5) integrin expression.

@entity263 :: ('MESH:D011471', 'Disease') :: ['prostate cancer'] @entity19988 :: ('MESH:C006680', 'Chemical') :: ['Baicalein'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity1426 :: ('MESH:D016718', 'Chemical') :: ['Arachidonic acid'] @entity11903 :: ('MESH:D019377', 'Chemical') :: ['12(S)-hydroxy-eicosatetraenoic acid'] @entity10327 :: ('239', 'Gene') :: ['platelet-type 12-lipoxygenase'] @entity11117 :: ('MESH:D006893', 'Chemical') :: ['hydroxy-eicosatetraenoic acids'] @entity11620 :: ('5122', 'Gene') :: ['PC3'] @entity45658 :: ('MESH:C085219', 'Chemical') :: ['N-benzyl-N-hydroxy-5-phenylpentamide']
@entity5 :: (MESH:D009369,Disease) :: ['tumor']
