Neuregulin/ @entity13719 -induced kinase signaling provides essential survival and protection clues for functional integrity of the adult heart and skeletal muscle. To define the regulatory pathways involved in neuregulin-dependent muscle cell survival, we set out to map the largely unknown transcript targets of this growth/differentiation factor in cardiocytes. Freshly isolated adult primary @entity35 cardiocytes were treated for 24 h with recombinant @entity1 neuregulin-1beta (NRG-1beta, 30 ng/ml). Transcript level alterations in NRG-1beta-treated and control cardiocytes (n = 6) were identified with Atlas @entity35 Toxicology 1.2 cDNA arrays (BD Clontech) and established permutation L1 regression analysis. Selected transcriptional adjustments were confirmed by RT-PCR and Western blotting. Involvement of MAPK pathways was verified with the inhibitor @entity4286 . Application of the single dose of NRG-1beta to quiescent cardiocytes induced expressional reprogramming of distinct cellular processes. This response included a prominent 50-100% increase in transcripts of multiple redox systems. It also involved a comparable mRNA augmentation of protein synthetic and folding factors together with augmented message for the trigger of @entity1283 , @entity12003 ( @entity12003 ). First evidence for a role of neuregulin in promotion of mitochondrial turnover, voltage-gated ion channel expression, and the suppression of @entity844 transporter mRNAs was revealed. Subsequent analysis confirmed a corresponding upregulation of redox factor proteins thioredoxin and the @entity62013 , @entity10625 , and @entity12003 and demonstrated downregulation of the related transcripts by @entity4286 in neuregulin-stimulated cultures. These MAPK-dependent expressional adjustments point to novel oxidative defense and @entity1184 pathways being involved in the longer lasting protective function of neuregulin in the heart.

Expressional reprogramming of survival pathways in XXXX cardiocytes by neuregulin-1beta.

@entity1 :: ('9606', 'Species') :: ['human'] @entity10625 :: ('24426(Tax:10116)', 'Gene') :: ['GSTP-1'] @entity844 :: ('CHEBI:35366', 'Chemical') :: ['fatty acid'] @entity4286 :: ('MESH:C093973', 'Chemical') :: ['PD-98059'] @entity1184 :: ('MESH:D006984', 'Disease') :: ['hypertrophy'] @entity1283 :: ('MESH:D006332', 'Disease') :: ['cardiac hypertrophy'] @entity13719 :: ('24337(Tax:10116)', 'Gene') :: ['ErbB2'] @entity12003 :: ('58919(Tax:10116)', 'Gene') :: ['cyclin D1', 'CCND1'] @entity62013 :: ('58819(Tax:10116)', 'Gene') :: ['thioredoxin reductase 1'] @entity35 :: ('10116', 'Species') :: ['rat', 'Rat']
@entity35 :: (10116,Species) :: ['rat']
