OBJECTIVE: The aim of this study was to examine the structural characterization of erythroid and megakaryocytic cell differentiation in Friend erythroleukemic cells using spectral imaging and electron microscopy. MATERIALS AND METHODS: Two variants of Friend @entity6007 cells were treated with @entity16747 ( @entity16747 ) to induce differentiation: 1) @entity17506 , which exhibit the normal phenotype and are susceptible to differentiation; and 2) the resistant R1 cells. The cells were analyzed by spectral imaging along with transmission and scanning electron microscopy. The expression of cell cycle regulatory proteins was analyzed by Western blotting. RESULTS: Spectral imaging of @entity16747 -treated @entity17506 and R1 cells stained by May-Gr  nwald-Giemsa and subjected to spectral similarity mapping revealed five morphologic cell types: proerythroblast-like cells, normoblast-like cells, reticulocyte-like cells, megakaryocytes, and apoptotic cells. In @entity17506 cells, both megakaryocytic differentiation characterized by nuclear lobes and erythroid differentiation characterized by accumulation of hemoglobin were detected; R1 cells were not committed to terminal differentiation. @entity16747 -induced cell cycle arrest at G(1) affected the expression of regulatory proteins in a similar manner in both types of cells. Expression of @entity8985 decreased and expression of @entity994 ( @entity994 ) increased. The level of the underphosphorylated form of @entity995 protein increased, inducing a decrease in the level of @entity517 . In addition, we detected a decrease in the expression of the anti-apoptotic regulator, @entity588 , and an increased expression of the pro-apoptotic regulator, @entity589 . CONCLUSIONS: Spectral imaging provides new insight for the morphologic characterization of erythroid and megakaryocytic cell differentiation as well as apoptosis. Image analysis was well correlated to cell cycle arrest and the expression of regulatory proteins.

Structural characterization of erythroid and megakaryocytic differentiation in Friend XXXX cells.

@entity589 :: ('581', 'Gene') :: ['Bax'] @entity588 :: ('596', 'Gene') :: ['Bcl-2'] @entity517 :: ('4609', 'Gene') :: ['c-myc'] @entity8985 :: ('1019', 'Gene') :: ['cyclin-dependent kinase 4'] @entity17506 :: ('MESH:D008557', 'Disease') :: ['MEL'] @entity6007 :: ('MESH:D004915', 'Disease') :: ['erythroleukemia'] @entity16747 :: ('MESH:C014026', 'Chemical') :: ['hexamethylene bisacetamide', 'HMBA'] @entity994 :: ('1026', 'Gene') :: ['p21', 'WAF1'] @entity995 :: ('MESH:D012175', 'Disease') :: ['phosphorylated retinoblastoma']
@entity6007 :: (MESH:D004915,Disease) :: ['erythroleukemia']
