OBJECTIVE: To assess the anti-inflammatory effects of an @entity859 analogue on lipopolysaccharide (LPS)-stimulated @entity1641 neutrophils. SAMPLE POPULATION: Neutrophils obtained from 10 healthy @entity1641 . PROCEDURES: An @entity859 analogue ( @entity19350 [ @entity19350 ]) was tested for its ability to inhibit production of reactive @entity26 species (ROS) in LPS-stimulated @entity1641 neutrophils. Selective @entity859 receptor antagonists were used to identify the receptor subtype responsible for effects. To assess the mechanism of action of @entity19350 , @entity268 concentrations were measured, and effects of @entity8279 (a stable analogue of @entity268 ) and rolipram (a type 4 phosphodiesterase inhibitor) were investigated. RESULTS: @entity19350 elicited concentration-dependent inhibition of ROS production that was inhibited by @entity42123 , a selective @entity859 A(2A) receptor antagonist; this effect of @entity19350 was not affected by the @entity859 A(2B) receptor antagonist @entity31182 . Also, @entity42123 blocked @entity19350 -induced increases in @entity268 concentrations, whereas @entity31182 did not alter this effect of @entity19350 . @entity7428 potentiated @entity19350 -induced inhibition of ROS production, and @entity8279 also inhibited ROS production. CONCLUSIONS AND CLINICAL RELEVANCE: Activation of @entity859 A(2A) receptors inhibited ROS production by LPS-stimulated @entity1641 neutrophils in a @entity268 -dependent manner. These results suggest that stable @entity859 A(2A) receptor agonists may be developed as suitable anti-inflammatory drugs in @entity1641 .

Effects of stimulation of XXXX A2A receptors on lipopolysaccharide-induced production of reactive @entity26 species by @entity1641 neutrophils.

@entity19350 :: ('MESH:D019830', 'Chemical') :: ['NECA', "5'-N-ethylcarboxamidoadenosine"] @entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity1641 :: ('9796', 'Species') :: ['horses', 'equine'] @entity859 :: ('MESH:D000241', 'Chemical') :: ['adenosine'] @entity42123 :: ('MESH:C097270', 'Chemical') :: ['ZM241385'] @entity7428 :: ('MESH:D020889', 'Chemical') :: ['Rolipram'] @entity31182 :: ('MESH:C496145', 'Chemical') :: ['MRS1706'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity8279 :: ('CHEBI:50095', 'Chemical') :: ['dibutyryl cAMP']
@entity859 :: (MESH:D000241,Chemical) :: ['adenosine']
