The method of @entity5019 immunodetection was used to map the distributions of neurons in the lumbar spinal dorsal horn that were activated following intracutaneous (i.c.) microinjection, or iontophoretic application, of different irritant chemicals to the lateral hindpaw of @entity35 . Microinjections (1 microl) of @entity112 , @entity750 ( @entity750 ), @entity1239 , @entity2951 , or @entity406 each elicited similar distributions of @entity5019 -like immunoreactivity (FLI) in laminae I-II of the ipsilateral superficial dorsal horn, with little or no FLI in deeper laminae or contralaterally. In laminae I and II, FLI cell counts were significantly higher following i.c. @entity112 , @entity750 , @entity2951 , @entity406 , and noxious pinch, compared to i.c. saline controls. @entity2951 -evoked FLI was dose-dependent. Multivariate analysis of variance revealed no significant difference in spatial distributions of FLI induced by any of the chemical or pinch stimuli. Iontophoretic application of @entity112 , @entity750 , or @entity1239 also elicited similar distributions of FLI in the superficial dorsal horn, and cell counts of FLI were significantly higher compared to controls receiving iontophoretic vehicle ( @entity5498 cellulose). These results indicate either that individual laminae I-II neurons are activated by each of the irritant chemicals, or that neurons selectively responsive to a given irritant are comingled without any apparent laminar segregation.

@entity5019 induction in XXXX superficial dorsal horn following cutaneous application of noxious chemical or mechanical stimuli.

@entity2951 :: ('MESH:D002211', 'Chemical') :: ['Capsaicin', 'capsaicin'] @entity1239 :: ('MESH:D009538', 'Chemical') :: ['nicotine'] @entity406 :: ('MESH:D005557', 'Chemical') :: ['formalin'] @entity5019 :: ('314322(Tax:10116)', 'Gene') :: ['Fos', 'c-fos'] @entity5498 :: ('CHEBI:32875', 'Chemical') :: ['methyl'] @entity750 :: ('MESH:D012701', 'Chemical') :: ['serotonin', '5-HT'] @entity112 :: ('MESH:D006632', 'Chemical') :: ['histamine'] @entity35 :: ('10116', 'Species') :: ['rats']
@entity35 :: (10116,Species) :: ['rat']
