OBJECTIVES: We aimed to investigate the protective effect of Lycium barbarum polysaccharides (LBPs) against oxidative stress-induced apoptosis and senescence in @entity1 lens epithelial cells. METHODS: To study apoptosis, SRA01/04 cells, a @entity1 lens epithelial cell lines, were exposed to 200   M @entity273 ( @entity274 ) for 24 h with or without pretreatment with LBPs. Cell viability was measured using a Cell Counting Kit-8 (CCK-8) assay. Cell apoptosis, intracellular reactive @entity26 species (ROS), and the loss of mitochondria membrane potential (/\  m) were detected by flow cytometric analyses. Expression levels of @entity588 and @entity589 proteins were measured by western blot analysis. The levels of @entity1234 ( @entity1865 ), @entity224 dismutase (SOD), and @entity2041 ( @entity2197 ) were quantized using commercial enzymatic kits according to the manufacturer's instructions. To study senescence, SRA01/04 cells were pre-incubated with LBPs and all cells were then exposed to 100   M @entity274 for 96 h. Cellular senescence was assessed by morphologic examination and senescence-associated b-galactosidase (SA-b-gal) staining. RESULTS: LBPs significantly reduced @entity274 -induced cell apoptosis, the generation of ROS, the loss of /\  m, and the levels of @entity1865 . LBPs also inhibited @entity274 -induced downregulated @entity588 and upregulated @entity589 proteins and increased the levels of SOD and @entity2197 enzyme activity. Moreover, LBPs significantly attenuated @entity274 -induced cellular senescence. CONCLUSIONS: These findings suggested that LBPs protect @entity1 lens epithelial cells from @entity274 -induced apoptosis by modulating the generation of ROS, loss of /\  m, @entity588 family, and antioxidant enzyme activity and attenuating cellular senescence.

Lycium barbarum Polysaccharides Protect XXXX Lens Epithelial Cells against Oxidative Stress-Induced Apoptosis and Senescence.

@entity1 :: ('9606', 'Species') :: ['human'] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H2O2'] @entity588 :: ('596', 'Gene') :: ['Bcl-2'] @entity589 :: ('581', 'Gene') :: ['Bax'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity2041 :: ('MESH:D005978', 'Chemical') :: ['glutathione'] @entity2197 :: ('CHEBI:16856', 'Chemical') :: ['GSH'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['hydrogen peroxide'] @entity1234 :: ('MESH:D008315', 'Chemical') :: ['malondialdehyde'] @entity224 :: ('MESH:D013481', 'Chemical') :: ['superoxide'] @entity1865 :: ('CHEBI:566274', 'Chemical') :: ['MDA']
@entity1 :: (9606,Species) :: ['Human']
