BACKGROUND: Presence at the same time of HBsAg and anti-HBs antibodies (HBsAg/Ab) is an entity sometimes encountered in @entity6194 ( @entity6194 ) carriers. OBJECTIVES: This study was designed to characterize such serological profiles and to assess the reliability of serological marker quantification by three commercially available assays in this setting. STUDY DESIGN: Among 2578 @entity6194 identified @entity1 , 129 (5%) had an HBsAg/Ab profile as determined by Abbott Architect. After exclusion of @entity325 ( @entity645 , @entity3531 , @entity44013 ), @entity97 reactivation or HBIg treatment, 101 samples from 62 @entity1 were tested for HBsAg and anti-HBs quantification using Architect, DiaSorin Liaison-XL and Roche Modular-Cobas. Influence of genotype and HBsAg variants was studied in 31 samples with @entity97 replication. RESULTS: HBsAg detection was confirmed with the 3 techniques for 98% (n=99) of the samples while the HBsAg/Ab profile was concordant between all techniques for 65% of them. The overall correlation between the 3 HBsAg quantification techniques was good (R(2): 0.94-0.97). The median HBsAg concentration was comparable for the 99 samples whatever the used technique but a bias of -0.11 and 0.02log IU/mL were noticed for DiaSorin and Roche compared to Abbott, respectively. Anti-HBs quantifications were poorly correlated between techniques with major discrepancies observed. Genotype and substitutions within the "a" determinant showed an impact on HBsAg quantification. CONCLUSIONS: The double HBsAg/Ab profile is not an analytical artifact and is confirmed on all commercially available techniques. While such profile does not influence HBsAg quantification, differences of HBsAg quantification were noticed according to @entity97 genotype or HBsAg variant.

Coexistence of circulating HBsAg and anti-HBs antibodies in XXXX carriers is not a simple analytical artifact and does not influence HBsAg quantification.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity97 :: ('10407', 'Species') :: ['HBV'] @entity3531 :: ('11096', 'Species') :: ['HCV'] @entity645 :: ('57667', 'Species') :: ['HIV'] @entity6194 :: ('MESH:D019694', 'Disease') :: ['chronic hepatitis B', 'CHB'] @entity44013 :: ('12475', 'Species') :: ['HDV'] @entity325 :: ('MESH:D060085', 'Disease') :: ['co-infections']
@entity6194 :: (MESH:D019694,Disease) :: ['chronic hepatitis B']
