OBJECT: The goal of this study was to illustrate the findings of a significant subpopulation of cells within a pediatric @entity10053 that have the specific cell surface phenotype found on known @entity1 neural stem cells. METHODS: Cells with a cell surface marker profile characteristic of @entity1 neural stem cells were isolated using fluorescence-activated cell sorting from a mostly nonmitotic @entity10053 removed from the brain of an 11-year-old @entity1 . An unusually high proportion (24%) of the cells were CD133 positive and @entity11707 , @entity2280 , and @entity1195 negative (CD133(+) @entity11707 (-) @entity2280 (-) @entity1195 (-) cells), the phenotypic antigenic pattern associated with neural stem cells; very few CD133-positive cells were not also @entity11707 , @entity2280 , and @entity1195 negative. Some cells (12%) were @entity2280 positive, indicating the presence within the @entity5 of hematopoietic stem cells. Cells formed cytospheres that resembled neurospheres when seeded into stem cell media and coexpressed beta-tubulin and @entity538 ( @entity538 ) but did not express the oligodendrocyte marker O4. Cell proliferation was demonstrated by incorporation of @entity1603 . The cells lost their capacity for self-renewal in vitro after four to six passages, although they continued to coexpress beta-tubulin and @entity538 . The cells did not differentiate into neurons or astrocytes when placed in differentiation medium. CONCLUSIONS: Although this @entity10053 contained a high proportion of phenotypic neural stemlike cells, the cells had limited proliferative capacity and multipotency. Their role in @entity10053 formation and growth is unknown.

Identification of phenotypic neural stem cells in a pediatric XXXX .

@entity1 :: ('9606', 'Species') :: ['girl', 'human'] @entity1603 :: ('MESH:D001973', 'Chemical') :: ['bromodeoxyuridine'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity1195 :: ('5788', 'Gene') :: ['CD45'] @entity11707 :: ('100133941', 'Gene') :: ['CD24'] @entity538 :: ('2670', 'Gene') :: ['GFAP', 'glial fibrillary acidic protein'] @entity10053 :: ('MESH:D018302', 'Disease') :: ['astroblastoma'] @entity2280 :: ('947', 'Gene') :: ['CD34']
@entity10053 :: (MESH:D018302,Disease) :: ['astroblastoma']
