cDNA clones encoding the alpha chain of the @entity19 lymphocyte-Peyer's patch adhesion molecule (LPAM), which is associated with lymphocyte homing, have been isolated by screening with the @entity1 VLA-4 (alpha 4h) probe. Several alpha 4 antigenic determinants were identified on COS-7 cells after transfection. From overlapping clones, approximately 5 kb of contiguous @entity377 sequence have been determined, encoding a protein sequence of 1039 @entity1522 for the LPAM alpha chain (alpha 4m). LPAM is a member of the integrin family of cell-surface heterodimers, and alpha 4m is the @entity19 homologue of the @entity1 alpha 4 h chain. The two proteins have a total sequence similarity of 84%, with an almost perfect conservation (31/32 @entity1522 ) in the cytoplasmic domain. Like alpha 4h, alpha 4m is distinct from other integrin alpha chains because it has neither an I-domain nor a @entity1473 -terminal cleavage site. The positions of the characteristic @entity462 residues are conserved, and a putative protease cleavage site is located near the middle of the protein sequence. The @entity2733 -terminal part of the protein contains seven homologous repeats, and three of them include putative divalent cation-binding sites. These sites are among the most conserved between the alpha 4m sequence and other alpha chains, and may therefore be involved in the binding of integrin alpha and beta chains. An additional cDNA clone was isolated which shares a sequence of perfect homology with the alpha 4m encoding cDNAs, but has a unique 3' @entity12564 end. This observation correlates with the fact that three discrete @entity19 RNA bands are observed in Northern blot experiments using alpha 4m as a probe, whereas only two @entity1 RNA species are described for alpha 4h, indicating a higher complexity for @entity19 than for @entity1 sequences.

Cloning and expression of cDNAs for the alpha subunit of the XXXX lymphocyte-Peyer's patch adhesion molecule.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity462 :: ('MESH:D003545', 'Chemical') :: ['Cysteine'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity377 :: ('CHEBI:36976', 'Chemical') :: ['nucleotide'] @entity2733 :: ('CHEBI:29345', 'Chemical') :: ['NH2'] @entity12564 :: ('MESH:D011061', 'Chemical') :: ['poly-A'] @entity1473 :: ('CHEBI:46883', 'Chemical') :: ['COOH']
@entity19 :: (10090,Species) :: ['murine']
