We have treated Caki-2 @entity1 @entity1358 in vivo using herpes simplex virus @entity1014 kinase (HSV-tk) gene therapy. Both stably transduced @entity5 , generated using retrovirus-mediated ex vivo HSV-tk gene transfer and direct intratumoral adenovirus-mediated HSV-tk gene transfer of wild @entity5 , were tested. Similar treatments with LacZ containing retro- and adenoviruses were used as controls. The outcome was evaluated by imaging the @entity5 before and after the treatment with magnetic resonance imaging, and using histology, immunocytochemistry, and survival analysis. When implanted orthotopically into nude @entity19 kidneys, Caki-2 cells formed reproducible @entity703 . In vivo magnetic resonance imaging provided an important tool for the evaluation of @entity5 growth. Transduction @entity5 in vivo with adeno-LacZ was 22+/-14%. Significant @entity5 regression was achieved with direct intratumoral adeno-HSV-tk transduction followed by intraperitoneal @entity4540 (GCV) (P<.001). Also, the treatment of stably transduced @entity5 with intraperitoneal GCV resulted in a significant treatment response in the HSV-tk group as compared to the LacZ group (P<.009). Increased apoptosis and macrophage infiltrations, reduced proliferation, and degenerative changes were observed in the @entity5 treated with HSV-tk and GCV. Also, significant prolongation in survival was achieved with adeno-HSV-tk- and GCV-treated @entity19 as compared to the controls. It is concluded that adeno-HSV-tk gene therapy may be useful for the treatment of @entity1358 in vivo.

HSV-tk gene therapy for @entity1 XXXX in @entity19 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity5 :: ('MESH:D009369', 'Disease') :: ['type tumors', 'tumor', 'efficiency of wild-type tumors', 'Caki-2 tumors', 'tumors'] @entity1358 :: ('MESH:D002292', 'Disease') :: ['renal cell carcinoma'] @entity1014 :: ('MESH:D013936', 'Chemical') :: ['thymidine'] @entity4540 :: ('MESH:D015774', 'Chemical') :: ['ganciclovir'] @entity703 :: ('MESH:D007680', 'Disease') :: ['cystic papillary kidney carcinomas']
@entity1358 :: (MESH:D002292,Disease) :: ['renal cell carcinoma']
