Leukocytes are critical effectors of @entity32 and @entity5 biology. Chemokine-like factors produced by such inflammatory sites are key mediators of @entity5 growth that activate leukocytic recruitment and @entity5 infiltration and suppress immune surveillance. Here we report that the endocrine peptide hormone, relaxin, is a regulator of leukocyte biology with properties important in recruitment to sites of @entity32 . This study uses the @entity1 monocytic cell line @entity1357 and normal @entity1 peripheral blood mononuclear cells to define a novel role for relaxin in regulation of leukocyte adhesion and migration. Our studies indicate that relaxin promotes adenylate cyclase activation, substrate adhesion, and migratory capacity of mononuclear leukocytes through a relaxin receptor @entity39210 -dependent mechanism. Relaxin-stimulated @entity268 accumulation was observed to occur primarily in non-adherent cells. Relaxin stimulation results in increased substrate adhesion and increased migratory activity of leukocytes. In addition, relaxin-stimulated substrate adhesion resulted in enhanced chemotaxis to @entity2842 . These responses in @entity1357 and peripheral blood mononuclear cells are relaxin dose-dependent and proportional to @entity268 accumulation. We further demonstrate that @entity39210 is critical for mediating these biological responses by use of RNA interference lentiviral short hairpin constructs. In summary, we provide evidence that relaxin is a novel leukocyte stimulatory agent with properties affecting adhesion and chemomigration.

Relaxin stimulates leukocyte adhesion and migration through a relaxin receptor XXXX -dependent mechanism.

@entity1 :: ('9606', 'Species') :: ['human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity1357 :: ('2736', 'Gene') :: ['THP-1'] @entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity39210 :: ('59350', 'Gene') :: ['LGR7'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity2842 :: ('6347', 'Gene') :: ['monocyte chemoattractant protein-1']
@entity39210 :: (59350,Gene) :: ['LGR7']
