The process of epithelial-mesenchymal transition (EMT), in addition to being an initiating event for @entity5 @entity3 , is implicated in conferring several clinically relevant properties to disseminating @entity5 cells. These include stem cell-like properties, resistance to targeted therapies and ability to evade immune surveillance. Enrichment analysis of gene expression changes during @entity2533 ( @entity2533 )-induced EMT in @entity2 cells identified complement cascade as one of the significantly enriched pathway. Further analysis of the genes in the complement pathway revealed an increase in the expression of complement inhibitors and a decrease in the expression of proteins essential for complement activity. In this study, we tested whether EMT confers resistance to complement-dependent @entity137 (CDC) in @entity2 cells and promotes @entity5 progression. @entity1009 is a potent inhibitor of membrane attack complex that mediates complement-dependent cell lysis. We observed a significant increase in the @entity1009 expression on the surface of cells after @entity2533 -induced EMT. Furthermore, @entity1009 knockdown restored susceptibility of cells undergoing EMT to cetuximab-mediated CDC. @entity2533 -induced @entity1009 expression during EMT is dependent on @entity7379 but not on @entity2536 . Chromatin immunoprecipitation analysis confirmed that @entity7379 directly binds to the @entity1009 promoter. Stable knockdown of @entity1009 in A549 cells inhibited experimental @entity3 . These results demonstrate that @entity2533 -induced EMT and @entity1009 expression confers an immune-evasive mechanism to disseminating @entity5 cells facilitating @entity5 progression. Together, our data demonstrates that @entity1009 inhibition may serve as an adjuvant to enhance the efficacy of antibody-mediated therapies, as well as to inhibit @entity2 .

Regulation of complement-dependent XXXX by @entity2533 -induced epithelial-mesenchymal transition.

@entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity7379 :: ('4088', 'Gene') :: ['Smad3'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity1009 :: ('966', 'Gene') :: ['CD59'] @entity2 :: ('MESH:D008175', 'Disease') :: ['lung cancer', 'metastasis in lung cancer'] @entity2536 :: ('4087', 'Gene') :: ['Smad2'] @entity2533 :: ('7040', 'Gene') :: ['TGF-b', 'transforming growth factor-b'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis']
@entity137 :: (MESH:D064420,Disease) :: ['cytotoxicity']
