BACKGROUND: @entity1073 ( @entity1073 ) is currently an incurable entity, and new therapeutic approaches are needed. We have applied a high-throughput phospho-proteomic technique to @entity1073 cell lines to identify activated pathways and we have then validated our data in both cell lines and @entity5 tissues. METHODS: PhosphoScan analysis was performed on @entity1073 cell lines. Results were validated by flow cytometry and western blotting. Functional validation was performed by blocking the most active pathway in @entity1073 cell lines. RESULTS: PhosphoScan identified more than 300 @entity669 -phosporylated proteins, among which many protein kinases. The most abundant peptides belonged to proteins connected with B-cell receptor (BCR) signaling. Active BCR signaling was demonstrated by flow cytometry in @entity1073 cells and by western blotting in @entity1073 tissues. Blocking BCR signaling by @entity10255 inhibitor @entity23939 induced dose/time-dependent apoptosis in @entity1073 cell lines, as well as several modifications in the phosphorylation status of BCR pathway members and a collapse of @entity4910 protein levels. CONCLUSION: Our data support a pro-survival role of BCR signaling in @entity1073 and suggest that this pathway might be a candidate for therapy. Our findings also suggest that @entity10255 activation patterns might be different in @entity1073 compared to other @entity706 subtypes.

Phospho-proteomic analysis of mantle cell XXXX cells suggests a pro-survival role of B-cell receptor signaling.

@entity706 :: ('MESH:D008223', 'Disease') :: ['lymphoma'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity4910 :: ('595', 'Gene') :: ['cyclin D1'] @entity23939 :: ('MESH:C041525', 'Chemical') :: ['piceatannol'] @entity10255 :: ('6850', 'Gene') :: ['Syk'] @entity1073 :: ('MESH:D020522', 'Disease') :: ['MCL tumor', 'Mantle cell lymphoma', 'MCL'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine']
@entity706 :: (MESH:D008223,Disease) :: ['lymphoma']
