The importance of @entity842 (NO) as a signaling molecule to various plant physiological and pathophysiological processes is becoming increasingly evident. However, little is known about how plants protect themselves from nitrosative and oxidative damage mediated by NO and NO-derived reactive @entity1568 species (RNS). @entity6710 , the product of the reaction between NO and @entity224 anion, is considered to play a central role in RNS-induced @entity137 , as a result of its potent ability to oxidize diverse biomolecules. Employing heterologous expression in bacteria and @entity375 , we investigated @entity6710 -scavenging activity in plants of 2- @entity2569 peroxiredoxin (2CPRX), originally identified as a @entity273 -reducing peroxidase that is ubiquitously distributed among organisms. The putative mature form of a chloroplast-localized 2CPRX from @entity958 was overproduced in @entity526 as an @entity1472 -terminally @entity37231 -tagged fusion protein. The purified recombinant 2CPRX, which was catalytically active as peroxidase, efficiently prevented the @entity6710 -induced oxidation of a sensitive compound. We also examined in vivo the ability of the @entity958 2CPRX to complement the 2CPRX deficiency of a @entity375 mutant. Functional expression in the mutant strain of the @entity958 2CPRX not only increased cellular tolerance to @entity273 , but also complemented the hypersensitive growth defect induced by @entity3542 -mediated @entity137 . The complemented cells significantly enhanced the capacity to reduce RNS-mediated oxidative damages. The results presented here demonstrate a new role of plant 2CPRX as a critical determinant of the resistance to RNS, and support the existence of a plant enzymatic basis for RNS metabolism.

Functional complementation in @entity375 reveals a protective role of chloroplast 2- @entity2569 peroxiredoxin against reactive XXXX species.

@entity37231 :: ('MESH:C471213', 'Chemical') :: ['hexahistidine'] @entity1568 :: ('MESH:D009584', 'Chemical') :: ['nitrogen'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity1472 :: ('CHEBI:46882', 'Chemical') :: ['amino'] @entity958 :: ('3702', 'Species') :: ['Arabidopsis thaliana', 'Arabidopsis'] @entity2569 :: ('CHEBI:17561', 'Chemical') :: ['Cys'] @entity3542 :: ('CHEBI:16301', 'Chemical') :: ['nitrite'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['hydrogen peroxide', 'hydroperoxide'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity6710 :: ('MESH:D030421', 'Chemical') :: ['Peroxynitrite', 'peroxynitrite'] @entity375 :: ('4932', 'Species') :: ['yeast', 'Saccharomyces cerevisiae'] @entity224 :: ('MESH:D013481', 'Chemical') :: ['superoxide'] @entity526 :: ('562', 'Species') :: ['Escherichia coli']
@entity1568 :: (MESH:D009584,Chemical) :: ['nitrogen']
