We have identified @entity2829 ( @entity2829 . inbred B73) mitochondrial homologs of the @entity526 molecular chaperones DnaK ( @entity44049 ) and @entity26540 (cpn60) using two-dimensional @entity3208 - @entity2975 gel electrophoresis and immunoblots. During heat stress (42 degrees C for 4 h), levels of @entity44049 and cpn60 proteins did not change significantly. In contrast, levels of two 22-kD proteins increased dramatically ( @entity70362 ). Monoclonal antibodies were developed to @entity2829 @entity44049 , cpn60, and @entity70362 . The monoclonal antibodies were characterized with regard to their cross-reactivity to chloroplastic, cytosolic, and mitochondrial fractions, and to different plant species. Expression of mitochondrial @entity70362 was evaluated with regard to induction temperature, time required for induction, and time required for degradation upon relief of stress. Maximal @entity70362 expression occurred in etiolated seedling mitochondria after 5 h of a +13 degrees C heat stress. Upon relief of heat stress, the @entity70362 proteins disappeared with a half-life of about 4 h and were undetectable after 21 h of recovery. Under continuous heat-stress conditions, the level of @entity70362 remained high. A cDNA for @entity2829 mitochondrial @entity70362 was cloned and extended to full length with sequences from an expressed sequence tag database. Sequence analysis indicated that @entity70362 is a member of the plant small heat-shock protein superfamily.

Heat-stress response of XXXX mitochondria.

@entity26540 :: ('13903475', 'Gene') :: ['GroEL'] @entity3208 :: ('MESH:D012967', 'Chemical') :: ['sodium dodecyl sulfate'] @entity2829 :: ('4577', 'Species') :: ['maize', 'Zea mays L'] @entity44049 :: ('542111(Tax:4577)', 'Gene') :: ['HSP70'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity70362 :: ('542602(Tax:4577)', 'Gene') :: ['HSP22'] @entity526 :: ('562', 'Species') :: ['Escherichia coli']
@entity2829 :: (4577,Species) :: ['maize']
