UNASSIGNED: @entity189 ( @entity189 ) is a serious threat to public health, and the biomarker discovery is of urgent needs. The data-independent mode (DIA) based sequential window acquisition of all theoretical fragment-ion spectra (SWATH) @entity3928 ( @entity3928 ) has been proved to be precise in protein quantitation and efficient for @entity5 biomarker researches. In this study, we performed the first SWATH- @entity3928 analysis comparing the @entity189 and normal tissues. Spike-in stable isotope labeling by @entity3534 in cell culture (super-SILAC) @entity3928 was used as a shotgun reference. We identified and quantified 1414 proteins across all SWATH- @entity3928 analyses. We found that SWATH- @entity3928 had a unique feature to preferentially detect proteins with smaller molecular weights than either super-SILAC @entity3928 or @entity1 proteome background. With SWATH- @entity3928 , 29 significant differentially express proteins (DEPs) were identified. Among them, @entity7464 ( @entity7464 ) was selected for further validation per novelty, @entity3928 quality and other supporting rationale. With the tissue microarray analysis, we found that @entity7464 had an AUC of 0.94 in differentiating @entity189 from normal tissue samples. In conclusion, SWATH- @entity3928 has unique features in proteome analysis, and it leads to the identification of @entity7464 as a potentially new diagnostic biomarker for @entity189 .

SWATH-based proteomics identified XXXX as a potential diagnosis biomarker for @entity189 .

@entity3534 :: ('MESH:D000596', 'Chemical') :: ['amino acids'] @entity1 :: ('9606', 'Species') :: ['human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity3928 :: ('MESH:C536030', 'Disease') :: ['mass spectrometry', 'MS'] @entity7464 :: ('760', 'Gene') :: ['carbonic anhydrase 2', 'CA2'] @entity189 :: ('MESH:D002277', 'Disease') :: ['Nasopharyngeal carcinoma', 'NPC']
@entity7464 :: (760,Gene) :: ['carbonic anhydrase 2']
