@entity1133 is the @entity1568 fertilizer most utilized in crop production worldwide. Understanding all factors involved in @entity1133 metabolism in plants is an essential step towards assessing and possibly improving the use of @entity1133 by plants. Urease, the enzyme responsible for @entity1133 hydrolysis, and its accessory proteins, necessary for nickel incorporation into the enzyme active site and concomitant activation, have been extensively characterized in bacteria. In contrast, little is known about their plant counterparts. This work reports a detailed characterization of @entity1460 UreG (GmUreG), a urease accessory protein. Two forms of native GmUreG, purified from seeds, were separated by metal affinity chromatography, and their properties (GTPase activity in absence and presence of @entity3411 or @entity8862 , secondary structure and metal content) were compared with the recombinant protein produced in @entity526 . The binding affinity of recombinant GmUreG (rGmUreG) for @entity3411 and @entity8862 was determined by isothermal titration calorimetry. rGmUreG binds @entity8862 or @entity3411 differently, presenting a very tight binding site for @entity8862 (K (d) = 0.02    0.01  M) but not for @entity3411 , thus suggesting that @entity8862 may play a role on the plant urease assembly process, as suggested for bacteria. Size exclusion chromatography showed that @entity8862 stabilizes a dimeric form of the rGmUreG, while NMR measurements indicate that rGmUreG belongs to the class of intrinsically disordered proteins. A homology model for the fully folded GmUreG was built and compared to bacterial UreG models, and the possible sites of interaction with other accessory proteins were investigated.

Biochemical and structural studies on native and recombinant XXXX UreG: a detailed characterization of a plant urease accessory protein.

@entity8862 :: ('CHEBI:37256', 'Chemical') :: ['Zn(2+)'] @entity1568 :: ('MESH:D009584', 'Chemical') :: ['nitrogen'] @entity1133 :: ('MESH:D014508', 'Chemical') :: ['Urea', 'urea'] @entity3411 :: ('CHEBI:49786', 'Chemical') :: ['Ni(2+)'] @entity1460 :: ('3847', 'Species') :: ['Glycine max'] @entity526 :: ('562', 'Species') :: ['Escherichia coli']
@entity1460 :: (3847,Species) :: ['Glycine max']
