Interleukin-1 receptor ( @entity10496 ) deficiency has been previously described in the dentate gyrus of autoimmune NZB and (NZB x NZW) F1 (or @entity28732 ) @entity19 . In this study, the genetic and molecular characterization of this defect were investigated in BWF2 @entity19 in relation to anti-DNA antibody production and microsatellite D1Nds4 (near the @entity34551 gene) polymorphism. @entity10496 density was quantified in the brain, spleen and pancreas, using in vitro quantitative autoradiography with recombinant @entity1 [125I]- @entity1192 as the ligand. This study of the dentate gyrus of F2 @entity19 revealed three phenotypes: NZW-like, NZB-like and F1-like, which occurred in a ratio of 1:1:2, with @entity10496 densities of 100%, 17% and 59%, respectively as compared to control NZW @entity19 (100%). In contrast, @entity10496 densities observed in the choroid plexus and peripheral organs were similar. Moreover a high production of @entity9749 anti-DNA antibodies was observed in F2 @entity19 , as in their parents, particularly those with the NZB-like phenotype. Microsatellite mapping of D1Nds4 revealed polymorphism in both parents and BWF2 @entity19 in relation to the level of @entity10496 density in the dentate gyrus. In spite of the acute defect in IL-1 binding in the dentate gyrus of NZB @entity19 , molecular analysis of @entity10496 mRNA (type I, II and accessory protein) showed similar amounts of mRNA, measured following RT-PCR amplification, in the hippocampal formation of both NZB and control C3H/He @entity19 . In conclusion, the transmission of the @entity10496 defect in the dentate gyrus of NZB @entity19 is monofactorial and the defect appears to be at the post-transcriptional level of @entity10496 synthesis. The lack of @entity10496 in the dentate gyrus seems to correlate with some autoimmune characteristics. Correlation of D1Nds4 polymorphism with the level of @entity10496 density suggests that it could be a predisposing gene to disease or a marker for other closely linked predisposing genes.

Interleukin-1 receptor deficiency in the hippocampal formation of (NZB x NZW)F2 XXXX : genetic and molecular studies relating to autoimmunity.

@entity1 :: ('9606', 'Species') :: ['human'] @entity34551 :: ('16177(Tax:10090)', 'Gene') :: ['IL1r1'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity10496 :: ('3554', 'Gene') :: ['IL-1R'] @entity1192 :: ('3552', 'Gene') :: ['IL-1alpha'] @entity28732 :: ('72145(Tax:10090)', 'Gene') :: ['BWF1'] @entity9749 :: ('668478(Tax:10090)', 'Gene') :: ['IgG2a']
@entity19 :: (10090,Species) :: ['mice']
