1. Methods of disrupting Krebs II @entity19 @entity5 cells have been studied. After washing the cells free of ions with @entity969 solutions, rapid disruption was obtained in @entity969 by use of an Ultra-Turrax disintegrator or a Dounce homogenizer. 2. Disruption of cells after osmotic shock led to the loss of proteins, especially cytochrome c, from the mitochondria. Such losses did not occur when cells were disrupted by shear in 0.3 m- @entity969 . 3. The distribution of protein, RNA, DNA, @entity5910 dehydrogenase, cytochrome c, cytochrome oxidase and succin-oxidase was measured in the various cell fractions after separation by differential centrifuging. 4. The mitochondrial fraction sedimented at 9500g was further fractionated by equilibrium sedimentation in a @entity969 gradient. The distribution of protein and enzyme activity in the gradient indicated that the 9500g pellet contains other material besides mitochondria. 5. Krebs-cell mitochondria contain up to five times as much RNA as do liver mitochondria. 6. After purification by equilibrium centrifugation Krebs-cell mitochondria still contain traces of DNA.

PROTEIN SYNTHESIS IN MITOCHONDRIA. 4. PREPARATION AND PROPERTIES OF MITOCHONDRIA FROM KREBS II XXXX ASCITES-TUMOUR CELLS.

@entity19 :: ('10090', 'Species') :: ['mouse'] @entity969 :: ('MESH:D013395', 'Chemical') :: ['sucrose'] @entity5 :: ('MESH:D009369', 'Disease') :: ['ascites-tumour'] @entity5910 :: ('MESH:C030298', 'Chemical') :: ['malate']
@entity19 :: (10090,Species) :: ['MOUSE']
