PURPOSE: Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinase (TIMPs) have been linked to the angiogenic process in general. In order to understand the potential roles of @entity11017 , @entity6929 and TIMPs in the @entity7499 process, we examined the expression and activities of @entity11017 , @entity6929 and TIMPs during the course of cauterization-induced @entity7499 in a @entity35 model. METHODS: Neovascularization of @entity35 corneas was induced by @entity7292 cauterization. The expression of @entity11017 , @entity6929 , @entity6930 and @entity11018 was examined by immunohistochemistry and RT-PCR. The protein activities of MMPs and TIMPs were compared in pre- and postcauterization corneas by gelatin zymography and reverse zymography, respectively. RESULTS: @entity11017 , @entity6930 and @entity11018 immunoreactivities were expressed in normal corneas, predominantly in the corneal epithelium. After injury, immunoreactivities of both MMPs and TIMPs were increased, notably in the healing corneal epithelium, infiltrating inflammatory cells, stromal fibroblasts and ingrowing vascular endothelial cells. The increase in gross @entity11017 enzymatic activity paralleled the maximal vascular ingrowth on day 4, while the gross @entity6929 enzymatic activity rose immediately on day 1, then decreased steadily, which paralleled the magnitude of @entity32 . The immunoreactivity of MMPs/TIMPs decreased significantly 2 weeks after cauterization. On day 35, @entity11017 , @entity6930 and @entity11018 staining was seen only in corneal epithelium and vascular endothelial cells. Both the RT-PCR and reverse zymography results revealed a more constant expression of @entity11018 , while the @entity6930 expression appeared to be more inducible. CONCLUSION: MMPs as well as TIMPs were upregulated in cauterization-induced @entity7499 , suggesting that both may participate in extracellular matrix remodeling in the corneal wound healing, @entity32 and neovascularization processes.

Expression of matrix metalloproteinases 2 and 9 and tissue inhibitors of metalloproteinase 1 and 2 in @entity32 -induced XXXX .

@entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation', 'inflammatory cell infiltration'] @entity6930 :: ('116510(Tax:10116)', 'Gene') :: ['TIMP-1'] @entity7499 :: ('MESH:D016510', 'Disease') :: ['corneal neovascularization'] @entity7292 :: ('MESH:D012835', 'Chemical') :: ['silver nitrate'] @entity11018 :: ('29543(Tax:10116)', 'Gene') :: ['TIMP-2'] @entity6929 :: ('81687(Tax:10116)', 'Gene') :: ['MMP-9'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity11017 :: ('81686(Tax:10116)', 'Gene') :: ['MMP-2']
@entity7499 :: (MESH:D016510,Disease) :: ['corneal neovascularization']
