A rapid, sensitive and specific high-performance liquid chromatography/electrospray ionization mass spectrometric (LC-ESI-MS) method was developed and validated for the quantification of @entity5319 , a major active constituent of Centella asiatica (L.) Urb. herbs, in @entity35 plasma. With @entity13575 as an @entity3538 ( @entity3538 ), a simple liquid-liquid extraction process was employed for the plasma sample preparation. @entity1172 was achieved within 6 min on a Shim-pack CLC-ODS column using @entity1626 and water (60:40, v/v) containing 0.1% (v/v) @entity5189 as the mobile phase. The detection was performed by MS with electrospray ionization interface in negative selected ion monitoring (SIM) mode. The linear range was 11-5500 ng/mL with the square regression coefficient (r(2) ) of 0.9995. The lower limit of quantification was 11 ng/mL. The intra- and inter- day precision ranged from 4.99 to 9.03%, and the accuracy was between 95.82 and 111.80%. The average recoveries of @entity5319 and @entity3538 from spiked plasma samples were >92%. The developed method was successfully applied to the pharmacokinetic study of @entity5319 in @entity35 after an oral administration.

Development and validation of high-performance liquid chromatography/electrospray ionization mass spectrometry for assay of @entity5319 in XXXX plasma and its application to pharmacokinetic study.

@entity3538 :: ('MESH:D002341', 'Disease') :: ['internal standard', 'IS'] @entity1626 :: ('MESH:C032159', 'Chemical') :: ['acetonitrile'] @entity5319 :: ('MESH:C093443', 'Chemical') :: ['madecassoside'] @entity13575 :: ('MESH:C015423', 'Chemical') :: ['paeoniflorin'] @entity1172 :: ('MESH:D001010', 'Disease') :: ['Chromatographic separation'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity5189 :: ('MESH:C030544', 'Chemical') :: ['formic acid']
@entity35 :: (10116,Species) :: ['rat']
