BACKGROUND: Cytokine storm generated by an alloimmune response after transplantation can lead to either graft survival or rejection. The aim of this study was to evaluate the serum levels of @entity688 , @entity4155 , transforming growth factor (TGF)-b1, and @entity379 and expression levels of @entity688 and @entity2533 in renal allograft recipients with or without donor bone marrow cell infusion (DBMI). METHODS: We retrospectively followed 28 living unrelated kidney recipients, including 14 with and 14 without DBMI infusion for 2 years. Also, 14 healthy subjects were included as a normal control group. PBMC gene expression analysis for mRNA levels of @entity688 and @entity2533 cytokines relative to b-actin as a reference gene was performed using quantitative fluorescence real-time polymerase chain reaction at the end of 2 years posttransplantation. Also, serum levels of @entity688 , @entity2533 , @entity379 , and @entity4155 in the 3 groups were measured by enzyme-linked immunosorbent assay at the same time. RESULTS: Both @entity1 groups showed increased gene expression and serum content of @entity688 compared with normal controls. The expression levels were only significant between control @entity1 and normal subjects (P=.02). Serum levels of @entity379 and @entity4155 were higher in untreated @entity1 compared with normal controls (P=.03 and P=.07, respectively). DBMI @entity1 showed significantly lower levels of serum @entity2533 and @entity4155 compared with normal subjects (P=.05 and P=.06, respectively). Also, infused @entity1 showed a positive correlation between circulating levels of @entity4155 and @entity688 (r=0.692; P=.006), and an inverse correlation between serum @entity1011 and @entity2533 levels (r=-0.580; P=.03). CONCLUSION: The decreased levels of inflammatory cytokines besides @entity688 with increased @entity2533 levels and better allograft function with improved clinical outcomes were observed among infused @entity1 , possibly indicating immunomodulatory effects of this approach in kidney allograft @entity1 .

Serum levels of @entity688 , @entity4155 , transforming growth factor (TGF)-b1, and interferon-y cytokines and expression levels of XXXX and @entity2533 genes in renal allograft recipients after donor bone marrow cell infusion.

@entity1 :: ('9606', 'Species') :: ['patients', 'patient'] @entity4155 :: ('3605', 'Gene') :: ['IL-17'] @entity2533 :: ('7040', 'Gene') :: ['TGF-b1'] @entity379 :: ('3458', 'Gene') :: ['IFN-y', 'interferon (IFN)-y'] @entity1011 :: ('MESH:D003404', 'Chemical') :: ['creatinine'] @entity688 :: ('3586', 'Gene') :: ['interleukin (IL)-10', 'IL-10']
@entity688 :: (3586,Gene) :: ['IL-10']
