UNASSIGNED: Shoot regeneration in calli derived from immature @entity2247 embryos is regulated by light conditions during the callus-induction period. @entity2247 cultivars Kanto Nijo-5 (KN5) and K-3 (K3) showed lower efficiency of shoot regeneration in a 16-h photoperiod during callus-induction than those in continuous darkness, whereas shoot regeneration was enhanced in cultures under a 16-h photoperiod in Golden Promise (GP) and Lenins (LN). These cultivars were classified as photo-inhibition type (KN5 and K3) or photo-induction type (GP and LN) according to their response to light. Contents of endogenous plant hormones were determined in calli cultured under a 16-h photoperiod and continuous darkness. In photo-inhibition type, higher accumulation of @entity2271 ( @entity2272 ) was detected in calli cultured under a 16-h photoperiod, whereas calli showed lower levels of endogenous @entity2272 in continuous darkness. However, cultivars of photo-induction type showed lower levels of @entity2272 in calli cultured under both light conditions, similarly to photo-inhibition type in continuous darkness. Exogenous @entity2272 inhibited the callus growth and shoot regeneration independent of light conditions in all cultivars. In photo-inhibition type, lower levels of endogenous @entity2272 induced by @entity2272 biosynthesis inhibitor, @entity28253 , reduced the photo-inhibition of shoot regeneration. Expression of @entity2272 biosynthesis gene, @entity69379 , in calli was regulated by the light conditions. Higher expression was observed in calli cultured under a 16-h photoperiod. These results indicate that @entity2272 biosynthesis could be activated through the higher expression of @entity69379 in a 16-h photoperiod and that the higher accumulations of @entity2272 inhibit shoot regeneration in the photo-inhibition type cultivars.

Light Inhibition of Shoot Regeneration Is Regulated by Endogenous XXXX Level in Calli Derived from Immature @entity2247 Embryos.

@entity2272 :: ('CHEBI:2365', 'Chemical') :: ['ABA'] @entity2271 :: ('MESH:D000040', 'Chemical') :: ['abscisic acid'] @entity2247 :: ('4513', 'Species') :: ['Barley', 'barley'] @entity28253 :: ('MESH:C013351', 'Chemical') :: ['fluridone'] @entity69379 :: ('778366(Tax:4513)', 'Gene') :: ['HvNCED1']
@entity2271 :: (MESH:D000040,Chemical) :: ['Abscisic Acid']
