OBJECTIVE: To explore the relation between @entity15073 and @entity125 and to evaluate the potential prognostic role of @entity15073 in invasive @entity150 ( @entity150 ). @entity1 AND METHODS: Fifty invasive @entity150 ( @entity150 ) tissue specimens, along with the adjacent bladder tissue specimens were obtained and clinical parameters of the fifty @entity1 were analyzed. Bioinformatics analysis was performed and microRNA-30a (miRNA-30a/ @entity15073 ) was selected as a potential miRNA targeting @entity125 and luciferase assay was performed to verify the binding site between @entity15073 and @entity125 . Quantitative real-time PCR (qRT-PCR) was conducted to assess the RNA expressions of @entity15073 and @entity125 , while western blotting and immunohistochemical staining were carried out to assess the protein expression of @entity125 . Finally, cell proliferation, cell cycle, cell migration and invasion assays were conducted to evaluate the cellular effects of @entity15073 and @entity125 on @entity150 cell lines T24 and 5637. RESULTS: @entity15073 was downregulated in @entity5 tissues when compared with adjacent bladder tissues (P<0.001), negatively correlating with @entity125 mRNA (R(2) =0.106, P=0.021) in invasive @entity150 and @entity15073 expression further decreased in @entity1 with shorter overall survival (OS) and disease-free survival (DFS) (P=0.039; P=0.037). Luciferase assay proved that @entity15073 inhibited @entity125 3'-untranslated region reporter activities in T24 and 5637 (P<0.001; P<0.001). Both @entity15073 and si- @entity125 negatively regulated cell proliferation (P=0.002 and P=0.035 in T24; P=0.029 and P=0.037 in 5637), activated cell cycle arrest (P<0.001 and P<0.001 in T24; P<0.001 and P<0.001 in 5637), and prevented cellular migration (P<0.001 and P<0.001 in T24; P=0.003 and P<0.001 in 5637) and invasion (P=0.009 and P=0.006 in T24; P=0.006 and P=0.002 in 5637) abilities. Ectopic @entity125 without 3'UTR partially rescued the above-mentioned cellular effects of over-expressed @entity15073 on @entity1 and 5637 cells. CONCLUSIONS: @entity15073 lightens cellular malignancy by antagonizing oncogene @entity125 and plays an efficient prognostic role in invasive @entity150 . This article is protected by copyright. All rights reserved.

MicroRNA-30a as a prognostic factor in Urothelial Carcinoma of Bladder inhibits cellular malignancy by antagonizing XXXX .

@entity1 :: ('9606', 'Species') :: ['patients', 'PATIENTS', 'T24'] @entity15073 :: ('407029', 'Gene') :: ['miR-30a', 'MiR-30a'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumour'] @entity125 :: ('4851', 'Gene') :: ['Notch1'] @entity150 :: ('MESH:D001749', 'Disease') :: ['UCB', 'urothelial carcinoma of bladder']
@entity125 :: (4851,Gene) :: ['Notch1']
