T cell activation is controlled by the coordination of stimulatory and negative regulatory signals which are not completely defined. In this study we tested for a possible direct effect of @entity5750 on the regulation of T cell activation and function. We show that soluble @entity5750 (sCD14) induces inhibition of antigen-mediated peripheral blood mononuclear cells (PBMC) proliferation and anti-CD3-mediated proliferation of @entity404 + @entity405 +, @entity404 + @entity405 + and @entity404 + @entity405 + Tcell clones. This effect is not due to cell death, but results from a marked inhibition of @entity1199 production. @entity1619 cell clones due to exogenous @entity1199 is not affected by sCD14. We also found that sCD14 inhibits production of another @entity1198 -like cytokine, @entity379 and a Th2-like cytokine, @entity3646 . Importantly, sCD14 induces a progressive accumulation of the inhibitory protein @entity4284 . We show that sCD14 binds to activated T cells. Following cell activation, biotinylated sCD14 stains CD3+ PBMC, as well as @entity1 T cell clones with varying intensity. The binding is saturable, can be inhibited by excess of unlabeled sCD14 and, following binding, sCD14 is internalized. Collectively, these findings reveal a previously unrecognized function of sCD14, namely its capacity to negatively regulate T lymphocyte activation and function by interacting directly with activated T cells.

Soluble XXXX acts as a negative regulator of @entity1 T cell activation and function.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1619 :: ('MESH:D015458', 'Disease') :: ['Proliferation of T'] @entity404 :: ('920', 'Gene') :: ['CD4'] @entity5750 :: ('929', 'Gene') :: ['CD14'] @entity4284 :: ('4792', 'Gene') :: ['IkappaB-alpha'] @entity1198 :: ('51497', 'Gene') :: ['Th1'] @entity379 :: ('3458', 'Gene') :: ['IFN-gamma'] @entity1199 :: ('3558', 'Gene') :: ['IL-2'] @entity3646 :: ('3565', 'Gene') :: ['IL-4'] @entity405 :: ('925', 'Gene') :: ['CD8']
@entity5750 :: (929,Gene) :: ['CD14']
