PURPOSE: Forty-eight @entity1 with high-risk re-sected stage III or IV @entity320 were immunized with two tumor antigen epitope peptides derived from @entity9857 (209-217)(210M) (IMDQVPSFV) and @entity11442 (368-376)(370D) (YMDGTMSQV) emulsified with incomplete Freund's adjuvant (IFA). @entity1 received peptides/IFA with or without interleukin (IL)-12 30 ng/kg to evaluate the @entity137 and immune responses in either arm with time to relapse and survival as secondary end points. @entity1 AND METHODS: Immunizations were administered every 2 weeks for 8 weeks, then every 4 weeks for 12 weeks, and then once 8 weeks later. A leukapheresis to obtain peripheral-blood mononuclear cells for immune analyses was done before and after vaccination. Skin testing with peptides and recall reagents was performed before and after vaccinations. RESULTS: @entity158 and @entity738 formation, fever, and lethargy of grade 1 or 2 were observed. Transient vaccine-related grade 3-but no grade 4- @entity137 was observed. Thirty-four of 40 @entity1 developed a positive skin test response to the @entity9857 peptide but none to @entity11442 . Immune responses were measured by release of gamma-interferon in an enzyme-linked immunosorbent assay (ELISA) by effector cells in the presence of peptide-pulsed antigen-presenting cells or by an antigen-specific tetramer flow cytometry assay. Thirty-three of 38 @entity1 demonstrated an immune response by ELISA after vaccination, as did 37 of 42 @entity1 by tetramer assay. Twenty-four of 48 @entity1 relapsed with a median follow-up of 20 months, and 10 @entity1 in this high-risk group have died. CONCLUSION: These data suggest a significant proportion of @entity1 with resected @entity320 mount an antigen-specific immune response against a peptide vaccine and indicate that IL-12 may increase the immune response and supporting further development of IL-12 as a vaccine adjuvant.

Effects of interleukin-12 on the immune response to a multipeptide vaccine for resected XXXX .

@entity1 :: ('9606', 'Species') :: ['Patients', 'patients', 'PATIENTS'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity', 'toxicities'] @entity9857 :: ('6490', 'Gene') :: ['gp100'] @entity738 :: ('MESH:D006099', 'Disease') :: ['granuloma'] @entity158 :: ('MESH:D010146', 'Disease') :: ['Local pain'] @entity11442 :: ('7299', 'Gene') :: ['tyrosinase'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma']
@entity320 :: (MESH:D008545,Disease) :: ['metastatic melanoma']
