Several genes that play roles in vacuolar biogenesis and targeting of proteins to vacuoles have been characterized in @entity375 . Although the vacuole is one of the most prominent compartments, little is known about molecular mechanism of vacuolar biogenesis in filamentous fungi. @entity7924 / @entity7924 , a small GTPase of the Rab/Ypt family in @entity375 , plays a vital role in homotypic vacuole fusion. We describe the isolation of the avaA gene from @entity14940 as a homologue of the @entity7924 / @entity7924 . Predicted 205 @entity1522 protein encoded by the avaA showed 67 and 72% identity with @entity375 @entity7924 / @entity7924 and @entity1 @entity27341 , respectively. The avaA disruptants exhibited highly fragmented vacuoles. We introduced mutations into the avaA gene, which alter @entity377 -binding characteristics of the gene products. Replacement of the avaA gene to the @entity3006 -bound form mutant resulted in fragmentation of vacuoles. Overexpression of the @entity3007 -bound form of avaA severely inhibited the hyphal growth and caused abnormal swelling of vacuoles. These results suggest that A. nidulans AvaA functions in the vacuolar biogenesis.

Cloning and characterization of a gene (avaA) from XXXX encoding a small GTPase involved in vacuolar biogenesis.

@entity1 :: ('9606', 'Species') :: ['human'] @entity7924 :: ('855012(Tax:4932)', 'Gene') :: ['VAM4', 'Ypt7p', 'YPT7', 'Vam4'] @entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity3007 :: ('MESH:D006160', 'Chemical') :: ['GTP'] @entity14940 :: ('162425', 'Species') :: ['Aspergillus nidulans'] @entity377 :: ('CHEBI:36976', 'Chemical') :: ['nucleotide'] @entity3006 :: ('MESH:D006153', 'Chemical') :: ['GDP'] @entity27341 :: ('7879', 'Gene') :: ['Rab7'] @entity375 :: ('4932', 'Species') :: ['yeast', 'Saccharomyces cerevisiae', 'S. cerevisiae']
@entity14940 :: (162425,Species) :: ['Aspergillus nidulans']
