OBJECTIVE: To investigate the expression of full-length and truncated @entity1465 / @entity10548 mRNA in @entity1 endometrium and decidua. DESIGN: In vitro experiment. SETTING: Tertiary university center. @entity1 (S): Premenopausal @entity1 undergoing hysterectomy because of benign diseases or induced abortions. INTERVENTION(S): Isolation of RNA from endometrial samples, reverse transcription, selective preamplification of full-length @entity1465 / @entity10548 mRNA and several shorter fragments of the receptor gene (exons 1-11, 1-10, and 1-5), nested polymerase chain reaction with internal primers. MAIN OUTCOME MEASURE(S): Appropriately sized cDNA product confirmed by sequencing. RESULT(S): All samples derived from the proliferative as well as from the early and mid-luteal phases were positive for all four amplification products, suggesting the expression of a full-length @entity1465 / @entity10548 mRNA. Only 5 of 8 samples derived from the late secretory phase and 2 of 12 samples derived from early decidua amplified the entire receptor sequence. In contrast, the shortest fragment (exons 1-5), coding for part of the extracellular receptor domain, was amplified in all samples. CONCLUSION(S): The data suggest cycle-dependent regulation of @entity1465 / @entity10548 mRNA by changes in the alternative splicing pattern and down-regulation of full-length @entity1465 / @entity10548 mRNA in early decidua. The major splicing site appears to be located between introns 5 and 9. Alternative splicing may be a mechanism regulating @entity1465 / @entity10548 down-regulation.

Evidence for cycle-dependent expression of full-length XXXX chorionic gonadotropin/luteinizing hormone receptor mRNA in @entity1 endometrium and decidua.

@entity1 :: ('9606', 'Species') :: ['women', 'human', 'PATIENT'] @entity10548 :: ('3973', 'Gene') :: ['LH receptor', 'LH-receptor'] @entity1465 :: ('93659', 'Gene') :: ['hCG']
@entity1 :: (9606,Species) :: ['human']
