PURPOSE: Platelet-activating factor ( @entity5567 ) is a potent signaling phospholipid that is found in @entity1 sperm and has a positive correlation with fertility. Whereas @entity5567 is present in @entity1 sperm, there are no relational reports on its content and the cells fertilization potential. Therefore, the study objective was to determine if @entity5567 content in capacitated-induced sperm is related to fertilization potential as determined by the sperm penetration assay (SPA). METHODS: Endogenous sperm lipids were measured for @entity5567 content by a specific radioimmunoassay following insemination of zona pellucida-free hamster ova. Data were analyzed by regression analysis and Student's t test. RESULTS: Regression analysis revealed a positive and significant relation (R2 = 0.806; P < 0.05) between @entity5567 content in @entity1 sperm and SPA outcome (pass: > or = 5.0; fail: < 5.0, penetrations/ova). @entity1 that passed (22.61 +/- 5.21 picomoles/10(6)) the SPA had significantly (P < 0.01) higher @entity5567 levels in their sperm than @entity1 that failed (12.91 +/- 1.76 picomoles/10(6) cells) the test. CONCLUSIONS: @entity5567 content in capacitated-induced sperm has a significant and positive relationship with fertilization potential. Fertilization potential may be predicted by measuring @entity5567 levels in capacitation-induced @entity1 sperm. Determining @entity5567 content in capacitated @entity1 sperm may be a beneficial diagnostic tool for the infertility specialist.

Fertilization potential of XXXX sperm is correlated with endogenous platelet-activating factor content.

@entity1 :: ('9606', 'Species') :: ['mammalian', 'Patients', 'human', 'patients'] @entity5567 :: ('CHEBI:52450', 'Chemical') :: ['PAF']
@entity1 :: (9606,Species) :: ['human']
