The variability of North American and Asian strains and isolates of @entity23634 was investigated. First, polymerase chain reaction (PCR) products representing the @entity40353 ( @entity40353 )-coding regions of 38 SMVs were analyzed for restriction fragment length polymorphisms (RFLP). Second, the @entity377 and predicted @entity535 sequence variability of the P1-coding region of 18 SMVs and the helper component/protease (HC/Pro) and @entity40353 -coding regions of 25 SMVs were assessed. The @entity40353 @entity377 and predicted @entity535 sequences were the most similar and predicted phylogenetic relationships similar to those obtained from RFLP analysis. Neither RFLP nor sequence analyses of the @entity40353 -coding regions grouped the SMVs by geographical origin. The @entity3881 /Pro sequences were more variable and separated the North American and Asian SMV isolates into two groups similar to previously reported differences in pathogenic diversity of the two sets of SMV isolates. The P1 region was the most informative of the three regions analyzed. To assess the biological relevance of the sequence differences in the HC/Pro and @entity40353 coding regions, the transmissibility of 14 SMV isolates by Aphis glycines was tested. All field isolates of SMV were transmitted efficiently by A. glycines, but the laboratory isolates analyzed were transmitted poorly. The @entity535 sequences from most, but not all, of the poorly transmitted isolates contained mutations in the aphid transmission-associated DAG and/or KLSC @entity535 sequence motifs of @entity40353 and HC/Pro, respectively.

Variability and transmission by Aphis glycines of North American and Asian XXXX isolates.

@entity3881 :: ('1423', 'Gene') :: ['P1 and HC'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity377 :: ('CHEBI:36976', 'Chemical') :: ['nucleotide'] @entity23634 :: ('12222', 'Species') :: ['Soybean mosaic virus'] @entity40353 :: ('64083', 'Gene') :: ['CP', 'coat protein']
@entity23634 :: (12222,Species) :: ['Soybean mosaic virus']
