UNASSIGNED: Identification and characterization of near-neighbor species are critical to the development of robust molecular diagnostic tools for biothreat agents. One such agent, @entity6943 , a soil bacterium and the causative agent of @entity6942 , is lacking in this area because of its genomic diversity and widespread geographic distribution. The @entity6402 genus contains over 60 species and occupies a large range of environments including soil, plants, rhizospheres, water, animals and @entity1 . The identification of novel species in new locations necessitates the need to identify the true global distribution of @entity6402 species, especially the members that are closely related to @entity6943 . In our current study, we used the @entity6402 -specific recA sequencing assay to analyze environmental samples from the Darwin region in the Northern Territory of Australia where @entity6942 is endemic. @entity6402 recA PCR negative samples were further characterized using 16s rRNA sequencing for species identification. Phylogenetic analysis demonstrated that over 70% of the bacterial isolates were identified as B. ubonensis indicating that this species is common in the soil where @entity6943 is endemic. Bayesian phylogenetic analysis reveals many novel branches within the @entity6402 complex, one novel B. oklahomensis-like species, and one novel branch containing one isolate that is distinct from all other samples on the phylogenetic tree. During the analysis with recA sequencing, we discovered 2 single @entity4334 polymorphisms in the reverse priming region of B. oklahomensis. A degenerate primer was developed and is proposed for future use. We conclude that the recA sequencing technique is an effective tool to classify @entity6402 and identify soil organisms in a @entity6942 endemic area.

Identification of XXXX Near-Neighbor Species in the Northern Territory of Australia.

@entity1 :: ('9606', 'Species') :: ['humans'] @entity6943 :: ('28450', 'Species') :: ['Burkholderia pseudomallei', 'B. pseudomallei'] @entity6942 :: ('MESH:D008554', 'Disease') :: ['melioidosis'] @entity4334 :: ('D009711', 'Chemical') :: ['nucleotide'] @entity6402 :: ('292', 'Species') :: ['B. cepacia', 'Burkholderia']
@entity6943 :: (28450,Species) :: ['Burkholderia pseudomallei']
