A novel, @entity2305 homologue, @entity46363 , has been detected in neural, epithelial, or hematopoietic cells but not in fibroblasts. We now demonstrate @entity46363 expression in a mesenchymal cell, @entity35 aortic smooth muscle cells (RSMC). @entity1808 ( @entity1808 ) or platelet-derived growth factor (PDGF-BB and PDGF-AA) markedly stimulated @entity46363 @entity669 phosphorylation in RSMC but did not affect @entity2305 phosphorylation. The PDGF-depedent @entity46363 @entity669 phosphorylation was slower and more prolonged than that of @entity1808 , correlating well with the differential effects of these agonists on cytosolic @entity460 ([ @entity141 ]i) signaling. An intracellular @entity460 chelator inhibited both the rapid and sustained activation of @entity46363 by @entity1808 and PDGF. Extracellular @entity460 chelation inhibited the PDGF-stimulated increase in @entity46363 @entity669 phosphorylation as well as the sustained (but not the early) activation by @entity1808 . In contrast, @entity2305 @entity669 phosphorylation was maximal in quiescent, adherent RSMC and was not affected by incubation with EGTA. Depletion of protein kinase C activity partially inhibited both the @entity1808 - and PDGF-induced @entity46363 @entity669 phosphorylation. Additional results confirm a relation between @entity46363 and the cytoskeleton. First, the @entity669 phosphorylation of paxilin correlated with activation of @entity46363 ; this increase was inhibited by @entity4527 . Second, @entity11119 blocked the PDGF- or @entity1808 -stimulated @entity669 phosphorylation of @entity46363 , suggesting a role for the cytoskeleton in agonist-dependent @entity46363 activation. Third, immunofluorescence analysis of @entity46363 localization demonstrated actin-like cytoskeleton staining extending into focal contacts. These results suggest that in mesenchymal cells, @entity46363 is localized to and activated by an actin cytoskeleton-dependent mechanism; a mechanism that is regulated in a @entity460 and protein kinase C-dependent manner independently of @entity2305 .

Regulation of a @entity460 -dependent @entity669 kinase in vascular smooth muscle cells by XXXX and platelet-derived growth factor. Dependence on @entity460 and the actin cytoskeleton.

@entity46363 :: ('50646(Tax:10116)', 'Gene') :: ['CADTK'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+'] @entity4527 :: ('MESH:D004533', 'Chemical') :: ['EGTA'] @entity1808 :: ('24179(Tax:10116)', 'Gene') :: ['Ang II', 'Angiotensin II'] @entity11119 :: ('MESH:D015638', 'Chemical') :: ['cytochalasin D'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity2305 :: ('25614(Tax:10116)', 'Gene') :: ['p125FAK'] @entity669 :: ('CHEBI:18186', 'Chemical') :: ['tyrosine']
@entity1808 :: (24179(Tax:10116),Gene) :: ['angiotensin II']
