@entity0 has attracted attention because of its relatively good prognosis, in spite of its high cytologic grade. It has, by definition, a consistent, florid @entity5 infiltrating lymphocyte (TIL) population, probably the result of cytotoxic T-lymphocytes recognizing @entity5 cells in an HLA-DR-restricted manner. HLA-DR tends to be more highly expressed on primary @entity189 cells than on @entity513 cells; however, the MHC-class II antigenicity of the @entity5 cells themselves has not been analyzed extensively, and as yet there has been no comparative study of HLA-DR expression in @entity513 metastatic to lymph nodes. Eleven cases of @entity189 and 15 cases of @entity513 , primaries, and respective lymph node @entity3 were analyzed by immunoperoxidase staining for HLA-DR and lymphocytes antigens. Polymerase chain reaction (PCR) analysis to identify HLA-DR subtypes from the @entity982 blocks was performed on selected cases of primaries and nodal @entity3 of both @entity5 types. Immunoperoxidase staining for HLA-DR antigen revealed a marked difference in antigen expression between @entity513 . In the @entity189 , the mean percentage of cells staining for HLA-DR was 74.5% in the @entity5 and 67.3% in the nodal @entity3 . For the @entity513 , the mean percentage of cells staining was 17.7% in the primaries and 7% in the @entity3 . There was a tendency for the level of HLA-DR expression to remain high in @entity189 metastatic to nodes, whereas whatever HLA-DR was present within ductal primaries tended to diminish when cells metastasized to regional nodes. PCR analysis of the HLA-DR within the two @entity5 types revealed no emerging subtype or variant that could be associated with either the medullary or the @entity513 . @entity189 cells express much greater quantities of HLA-DR, on the whole, than @entity513 . Expression of HLA-DR is retained on @entity189 cells that have spread to lymph nodes, whereas the smaller quantities of HLA-DR present within ductal primaries tend to diminish even further when the @entity5 cells are found in lymph nodes. No discernible HLA-DR mutations or predominant subtypes emerged on PCR analysis, and the authors therefore conclude that it is the quantity and not the quality of HLA-DR expression in @entity189 that maintains the characteristic TIL infiltrate, not seen in @entity513 .

Antigenic characterization of XXXX of the breast: HLA-DR expression in lymph node positive cases.

@entity0 :: ('MESH:D001943', 'Disease') :: ['Medullary carcinoma of the breast'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'primary tumors'] @entity189 :: ('MESH:D002277', 'Disease') :: ['Medullary carcinoma', 'medullary carcinoma', 'medullary carcinomas'] @entity513 :: ('MESH:D044584', 'Disease') :: ['ductal carcinomas', 'medullary and ductal carcinomas', 'ductal carcinoma'] @entity982 :: ('MESH:D010232', 'Chemical') :: ['paraffin'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastases']
@entity189 :: (MESH:D002277,Disease) :: ['medullary carcinoma']
