To assess the level of heterozygosity within two commonly used inbred mapping @entity3392 strains, @entity69452 and SJD, we genotyped polymorphic CA-repeat markers randomly dispersed throughout the @entity3392 genome. (For clarity purposes we will primarily use the term polymorphic to define polymorphism between strains, and the term heterozygous to address heterogeneity within a strain.) Eight male individuals each from @entity69452 and SJD stocks were typed for 235 and 183 markers, respectively. Over 90% of the markers typed were polymorphic between these two strains. We found a limited number of heterozygous markers persisting in clusters within each inbred line. In the SJD strain, these were mainly limited to a few telomeric regions or regions otherwise distant from centromeres. As expected, centromeric regions were homozygous in the SJD strain, consistent with its derivation from a single half-tetrad individual. In contrast, heterozygous clusters were distributed randomly throughout the genome in the @entity69452 strain, and these clusters could be detected with linked polymorphic markers. Nevertheless, most regions of the @entity69452 strain are homozygous for CA-repeat markers in current stocks. This identification of the heterozygous regions within @entity69452 and SJD lines should permit rapid fixation of these remaining regions in future generations of inbreeding. In addition, we established levels of polymorphism between the inbred, @entity69452 and SJD, strains and three other commonly used strains, the *AB, WIK, and Florida wild type (hereafter referred as EKK), with CA-repeat markers as well as SSCP polymorphisms. These data will maximize the use of these strains in mapping experiments.

Assessment of polymorphism in XXXX mapping strains.

@entity69452 :: ('30491(Tax:7955)', 'Gene') :: ['C32'] @entity3392 :: ('7955', 'Species') :: ['zebrafish']
@entity3392 :: (7955,Species) :: ['zebrafish']
