Cytosolic and nuclear @entity172 receptor forms of @entity1573 oviduct have been studied by (1) measuring hormone dissociation kinetics and by (2) sucrose density gradient analysis on high salt gradients. @entity53 dissociates from the receptor in @entity1573 oviduct cytosol at 22 degrees C following a two-phase exponential process. The fraction of receptor with a fast dissociation rate (k = 120 X 10(-3) min-1) decreases as a function of the pre-incubation at 22 degrees C; after prolonged pre-incubation only the slowly dissociating (k = 12.3 X 10(-3) min-1) form remains. Dissociation of @entity68665 , a synthetic @entity172 with a higher affinity, from the cytosol receptor at 30 degrees C is similar, showing a transition of a fast dissociating form (k = 120 X 10(-3) min-1) to a slowly dissociating form (k = 7.6 X 10(-3) min-1) as a result of pre-incubation at 30 degrees C. A concomitant temperature-dependent shift of the @entity172 receptor from a 4.8 S to a 6.1 S form was observed with @entity68665 but not with @entity53 as a ligand. Sodium molybdate (20 mM) and @entity13672 (400 mM) inhibit the temperature-dependent increase in sedimentation coefficient, but molybdate allows the formation of a receptor form which shows intermediary dissociation kinetics. @entity172 receptor, precipitated with @entity1329 (0-35%) shows monophasic dissociation kinetics of @entity53 (k = 39.5 X 10(-3) min-1) and for @entity68665 (k = 10.8 X 10(-3) min-1), suggesting full receptor activation only with @entity68665 as a ligand. @entity68665 -receptor complexes obtained by @entity1329 precipitation sediment at 0 degree C at 4.8 S. Only after subsequent incubation at 30 degrees C a shift from 4.8 S to 5.9 S is observed, suggesting that the formation of the slowly dissociating form of the receptor may precede the formation of a stable transformed receptor complex. The nuclear @entity172 receptor with @entity53 as a ligand shows biphasic dissociation kinetics at 22 degrees C (k = 70 X 10(-3) min-1; k = 14.0 X 10(-3) min-1). The ratio of both components (1:1) does not change after preincubation of the nuclear receptor extract at 22 degrees C. @entity68665 dissociates from the nuclear receptor at 30 degrees C monophasically with a slow rate (k = 6.1 X 10(-3) min-1), suggesting that it is extracted as an activated hormone-receptor complex.(ABSTRACT TRUNCATED AT 400 WORDS)

@entity172 receptor in XXXX oviduct: receptor dissociation kinetics and transformation.

@entity172 :: ('MESH:D004967', 'Chemical') :: ['estrogen', 'Estrogen'] @entity13672 :: ('MESH:C024553', 'Chemical') :: ['NaSCN'] @entity68665 :: ('MESH:C011310', 'Chemical') :: ['Moxestrol', 'moxestrol'] @entity53 :: ('MESH:D004958', 'Chemical') :: ['Estradiol', 'estradiol'] @entity1573 :: ('9031', 'Species') :: ['chicken'] @entity1329 :: ('MESH:D000645', 'Chemical') :: ['ammonium sulfate']
@entity1573 :: (9031,Species) :: ['chicken']
