An aberrant interaction between hematopoietic stem cells and mesenchymal stromal cells has been linked to disease and shown to contribute to the pathophysiology of @entity1583 in @entity19 models. @entity12188 is an aggressive @entity5 affecting young @entity1 . Here we investigated the impact of @entity12188 on mesenchymal stromal cells. Mesenchymal stromal cells were expanded from bone marrow samples of @entity1 at diagnosis (n=9) and after hematopoietic stem cell transplantation (n=7; from 5 @entity1 ) and from healthy @entity1 (n=10). Cells were characterized by phenotyping, differentiation, gene expression analysis (of controls and samples obtained at diagnosis) and in vitro functional studies assessing immunomodulation and hematopoietic support. Mesenchymal stromal cells from @entity1 did not differ from controls in differentiation capacity nor did they differ in their capacity to support in vitro hematopoiesis. Deep- @entity12337 sequencing revealed differential mRNA expression in @entity1 -derived samples, including genes encoding proteins involved in immunomodulation and cell-cell interaction. Selected gene expression normalized during remission after successful hematopoietic stem cell transplantation. Whereas natural killer cell activation and peripheral blood mononuclear cell proliferation were not differentially affected, the suppressive effect on monocyte to dendritic cell differentiation was increased by mesenchymal stromal cells obtained at diagnosis, but not at time of remission. This study shows that active @entity12188 affects the immune response-related gene expression and function of mesenchymal stromal cells. In contrast, the differential gene expression of hematopoiesis-related genes could not be supported by functional data. Decreased immune surveillance might contribute to the therapy resistance and progression in @entity12188 .

Gene-expression and in vitro function of mesenchymal stromal cells are affected in XXXX .

@entity1 :: ('9606', 'Species') :: ['children', 'patients', 'patient', 'infants'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity5 :: ('MESH:D009369', 'Disease') :: ['malignant disease'] @entity12188 :: ('MESH:D054429', 'Disease') :: ['Juvenile myelomonocytic leukemia', 'juvenile myelomonocytic leukemia'] @entity1583 :: ('MESH:D019337', 'Disease') :: ['hematologic malignancies'] @entity12337 :: ('55511', 'Gene') :: ['SAGE']
@entity12188 :: (MESH:D054429,Disease) :: ['juvenile myelomonocytic leukemia']
