The response to treatment and the malignant progression of @entity5 are influenced by the ability of the @entity5 cells to withstand severe @entity1949 during prolonged exposure to @entity519 at normal or low extracellular pH (pHe). The objective of the present work was to demonstrate intertumour heterogeneity under conditions of microenvironment-induced @entity1949 and to investigate whether the heterogeneity can be attributed to differences in the capacity of the @entity5 cells to generate energy in an @entity26 -deficient microenvironment. Cultures of four @entity1 @entity320 cell lines (BEX-c, COX-c, SAX-c, WIX-c) were exposed to @entity519 in vitro at pHe 7.4, 7.0 or 6.6 for times up to 31 h by using the steel-chamber method. High-performance liquid chromatography was used to assess adenylate energy charge as a function of exposure time. Cellular rates of @entity413 uptake and @entity880 release were determined by using standard enzymatic test kits. The adenylate energy charge decreased with time under @entity519 in all cell lines. The decrease was most pronounced shortly after the treatment had been initiated and then tapered off. BEX-c and SAX-c showed a significantly higher adenylate energy charge under @entity519 conditions than did COX-c and WIX-c whether the pHe was 7.4, 7.0 or 6.6, showing that @entity5 can differ in the ability to avoid @entity1949 during microenvironmental stress. There was no correlation between the adenylate energy charge and the rates of @entity413 uptake and @entity880 release. Intertumour heterogeneity in the ability to withstand @entity1949 in an @entity26 -deficient microenvironment cannot therefore be attributed mainly to differences in the capacity of the @entity5 cells to generate energy by anaerobic metabolism. The data presented here suggest that the heterogeneity is rather caused by differences in the capacity of the @entity5 cells to reduce the rate of energy consumption when exposed to @entity519 .

Energy metabolism in @entity1 @entity320 cells under XXXX and acidic conditions in vitro.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1949 :: ('MESH:D012892', 'Disease') :: ['energy deprivation'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumours', 'tumour'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity519 :: ('MESH:D000860', 'Disease') :: ['hypoxic', 'hypoxia'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity320 :: ('MESH:D008545', 'Disease') :: ['melanoma']
@entity519 :: (MESH:D000860,Disease) :: ['hypoxic']
