@entity5 cell-stromal cell interaction plays a crucial role in the malignant growth of @entity5 cells. In the skin, the main stromal cell types consist of dermal fibroblasts and subcutaneous adipocytes. Fibroblasts are shown to promote the invasive growth of various @entity5 cell types. The interaction between @entity5 cells and stromal adipocytes, however, has not been sufficiently studied even in cutaneous @entity189 . To address the effects of adipocytes on the biologic behavior of @entity5 cells, we examined the growth and differentiation of a @entity957 cell line of the skin (DJM-1), using a three-dimensional collagen gel matrix culture with a cutaneous environmental factor, air exposure. The growth was estimated by the uptake of @entity1603 ( @entity1603 ) for 24 h. The @entity1603 indices of DJM-1 cells in stromal-cell-free, fibroblast-containing, and adipocyte- containing conditions were 19.7 +/- 1.9%, 19.8 +/- 2.8%, and 4.7 +/- 1.4%, respectively, whereas the @entity1603 index on the gel containing both fibroblasts and adipocytes was 10.4 +/- 3.3%. In terms of differentiation, DJM-1 cells cocultured with adipocytes constructed the best-organized stratified layer with a cornified-like structure in all conditions above. The differentiation markers involucrin and @entity7434 were immunohistochemically detected in this structure of DJM-1 cells. Adipocyte-induced phenomena were not affected distinctively by air exposure. These results indicate that adipocytes, but not fibroblasts, promote the differentiation of @entity957 cells (DJM-1) and inhibit their growth. These adipocyte-induced phenomena were not completely inhibited by fibroblasts. In conclusion, we suggest that stromal adipocytes may be involved in the differentiating mechanisms of cutaneous @entity189 cells.

Subcutaneous adipocytes promote the differentiation of XXXX cell line (DJM-1) in collagen gel matrix culture.

@entity1603 :: ('MESH:D001973', 'Chemical') :: ['BrdU', 'bromodeoxy-uridine'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer', 'Cancer'] @entity957 :: ('MESH:D002294', 'Disease') :: ['squamous cell carcinoma'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinoma'] @entity7434 :: ('3858', 'Gene') :: ['cytokeratin 10']
@entity957 :: (MESH:D002294,Disease) :: ['squamous cell carcinoma']
