Neurocalcin (NC), a neuron-specific EF-hand @entity141 -binding protein, purified from @entity291 brain [Terasawa, Nakano, Kobayashi and Hidaka (1992) J. Biol. Chem. 267, 19596-19599] contains multiple isoforms. We previously cloned NCdelta from @entity291 brain and showed high expression in neuronal tissues [Okazaki, Watanabe, Ando, Hagiwara, Terasawa and Hidaka (1992) Biochem. Biophys. Res. Commun. 185, 147-153]. We report here the molecular cloning and expression of a cDNA encoding @entity291 brain NCalpha. The translated @entity291 protein is 191 @entity1522 long and shares 69.1% of its @entity535 sequence with NCdelta. Recombinant NCalpha migrates as a single 23 kDa band and exhibits a @entity141 -dependent mobility shift on @entity214 /PAGE. Analysis of fluorescence emission spectra showed the @entity141 -induced peak at 337 nm. Interestingly, the mobility shift and the fluorescence intensity at 337 nm were larger for NCalpha than for NCdelta. In @entity141 -overlay experiments, however, the apparent affinity of NCalpha for 45Ca2+ was similar to that of NCdelta. Immunohistochemical analysis revealed NCalpha expression in the granular layer of the @entity35 cerebellar cortex whereas NCdelta was found in the Purkinje cell layer. In the @entity35 olfactory bulb, NCalpha was located in external tufted cells, and NCdelta was found in the periglomerular cells. These data demonstrate that NC isoforms differ in their tissue distribution and conformational changes induced by @entity141 binding. Thus differential regulation of the two NC isoforms may be involved in control of neuron function.

Cloning and expression of a cDNA encoding a new neurocalcin isoform (neurocalcin alpha) from XXXX brain.

@entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity141 :: ('CHEBI:29108', 'Chemical') :: ['Ca2+'] @entity291 :: ('9913', 'Species') :: ['bovine'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS']
@entity291 :: (9913,Species) :: ['bovine']
