UNASSIGNED: Dairy @entity291 fertility has declined over time due to factors including reduced fertilization and early @entity2085 . To counter fertility problems and better study preimplantation embryonic development, in vitro production systems have been developed. These systems largely assess embryos based on their morphology, which is not a strong indicator of developmental potential. Currently, no biomarkers can be used to noninvasively survey an embryo's potential in terms of its development and ability to establish a pregnancy. Thus, the objective of this study was to characterize and identify microRNA (miRNA) in culture media of embryos of differing developmental competence for future development as noninvasive biomarkers of embryo quality. The MiRNA sequencing of media conditioned by blastocyst and degenerate (those that failed to develop from the morula to blastocyst stage) embryos, revealed 11 differentially expressed miRNA; all were higher in concentration in degenerate conditioned media. Differential expression of mature microRNA (miR)-24, @entity69539 , and @entity69540 was further validated using quantitative real-time PCR. Functional analysis of miR-24 revealed that addition of a mimic miRNA to culture media of morulae embryos resulted in a 27.3% decrease in development to the blastocyst stage. Furthermore, expression of miR-24 was 44.29-fold higher in blastocysts cultured with a miR-24 mimic compared with control blastocysts. Interestingly, the expression of @entity69541 , a target gene of miR-24 was repressed in embryos grown in the presence of the miRNA mimic. Mimic supplementation experiments suggest that miRNA are taken up by the embryo and that extracellular miRNA affect embryonic development. Overall, identification of a rich extracellular milieu in conditioned media sets the framework for future studies to determine the long-term predictive ability of embryo-based miRNA biomarkers on pregnancy outcome.

Characterization of microRNA in XXXX in vitro culture media associated with embryo quality and development.

@entity2085 :: ('MESH:D020964', 'Disease') :: ['embryonic loss'] @entity69539 :: ('791033(Tax:9913)', 'Gene') :: ['miR-191'] @entity69540 :: ('790977(Tax:9913)', 'Gene') :: ['miR-148a'] @entity69541 :: ('512613(Tax:9913)', 'Gene') :: ['CDKN1b'] @entity291 :: ('9913', 'Species') :: ['cattle']
@entity291 :: (9913,Species) :: ['bovine']
