Insulin is normally secreted in @entity1 in regular pulses every 5 to 15 minutes. @entity74 has been demonstrated in several insulin-resistant states and it is unclear whether this represents a primary beta-cell defect contributing to impairment of peripheral insulin action or rather is a consequence of insulin resistance. Basal or near basal insulin administration by pulsatile infusion augments hypoglycemic effect and improves insulin-mediated @entity413 uptake compared with insulin by continuous infusion. To date no study has examined whether normal basal insulin pulsatility is required to preserve @entity1720 during @entity3190 . We studied the effect of overnight pulsatile versus continuous basal insulin on a subsequent hyperinsulinemic euglycemic clamp. Nineteen normal volunteers (male:female ratio, 17:2; mean age +/- SEM, 26.1 +/- 2.3 years) were studied on 2 occasions each. Endogenous insulin secretion was inhibited by @entity2390 (0.43 microg kg(-1). h(-1)) and replaced overnight at 5.4 mU kg(-1). h(-1) either by continuous infusion or in 2-minute pulses every 13 minutes (n = 10) or every 7 minutes (n = 9). Glucagon was replaced at physiological concentration by continuous infusion (30 ng. kg(-1). h(-1)). Venous plasma @entity413 overnight was not significantly different between the pulsatile and continuous protocols. After discontinuing the overnight insulin infusion, insulin action was assessed during a hyperinsulinemic euglycemic clamp (1 mU kg(-1). h(-1)). @entity413 infusion rates at steady-state during the hyperinsulinemic clamp were similar between continuous and both frequencies of pulsatile infusion (continuous 44.6 +/- 4.3 micromol. kg(-1). @entity1009 ) v 13-minute pulsatile 41.7 +/- 5.9 micromol. kg(-1). @entity1009 ), P =.27; continuous 34.6 +/- 2.5 micromol. kg(-1) @entity1009 ) v 7-minute pulsatile 41.4 +/- 3.2 micromol. kg(-1). @entity1009 ), P =.08). We conclude that overnight pulsatile compared with continuous insulin administration has no different effect on subsequent peripheral insulin-mediated @entity413 uptake. A priming effect cannot therefore explain the previously demonstrated association between endogenous insulin pulse frequency and peripheral insulin action.

Comparison of the priming effects of pulsatile and continuous insulin delivery on insulin action in XXXX .

@entity1 :: ('9606', 'Species') :: ['man'] @entity1009 :: ('966', 'Gene') :: ['min(-1'] @entity3190 :: ('MESH:D006946', 'Disease') :: ['hyperinsulinemia'] @entity74 :: ('MESH:D030342', 'Disease') :: ['Disordered pulsation'] @entity2390 :: ('MESH:D015282', 'Chemical') :: ['octreotide'] @entity1720 :: ('MESH:D007333', 'Disease') :: ['subsequent insulin sensitivity'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose', 'Glucose']
@entity1 :: (9606,Species) :: ['man']
