UNASSIGNED: The CreBC ( @entity505 source responsive) two-component regulation system of @entity526 affects a number of functions, including intermediary @entity505 catabolism. The impact of different creC mutations (a   creC mutant and a mutant carrying the constitutive creC510 allele) on bacterial physiology was analyzed in @entity413 cultures under three @entity26 availability conditions. Differences in the amounts of extracellular metabolites produced were observed in the null mutant compared to the wild-type strain and the mutant carrying creC510, and shown to be affected by @entity26 availability. The   creC strain secreted more formate, @entity4588 , and @entity598 , but less @entity880 in low aeration. These metabolic changes were associated to differences in AckA and LdhA activities, both of which were affected by CreC. Measurement of the @entity1110 / @entity1110 ratios showed that the creC510 strain had a more reduced intracellular redox state, while the opposite was observed for the   creC mutant, particularly at intermediate @entity26 availability conditions, indicating that CreC affects redox balance. The null mutant formed more @entity4588 than the wild-type strain in both low aeration and no aeration. Over-expression of the genes encoding @entity7954 carboxylase from @entity526 and a @entity2125 -forming @entity5189 dehydrogenase from Candida boidinii in the   creC mutant further increased the yield of @entity4588 on @entity413 . Interestingly, the elimination of ackA and adhE did not improve significantly the production of @entity4588 . The diverse metabolic effects of this regulator on the central biochemical network of @entity526 make it a good candidate for metabolic engineering manipulations to enhance the formation of bioproducts such as @entity4588 .

The CreC regulator of XXXX , a new target for metabolic manipulations.

@entity2125 :: ('MESH:D009243', 'Chemical') :: ['NADH'] @entity4588 :: ('MESH:D019802', 'Chemical') :: ['succinate'] @entity7954 :: ('MESH:D010728', 'Chemical') :: ['phosphoenolpyruvate'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity598 :: ('CHEBI:47622', 'Chemical') :: ['acetate'] @entity1110 :: ('MESH:D009249', 'Chemical') :: ['NAD(P)H', 'NAD(P)(+)'] @entity5189 :: ('MESH:C030544', 'Chemical') :: ['formate'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity505 :: ('MESH:D002244', 'Chemical') :: ['carbon'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose'] @entity526 :: ('562', 'Species') :: ['Escherichia coli', 'E. coli']
@entity526 :: (562,Species) :: ['Escherichia coli']
