Lipopolysaccharide-( @entity558 ) binding proteins present on @entity19 -lymphocyte and macrophage-like cell lines were identified by a ligand-blotting method and subsequent immunological detection of bound @entity558 . Membrane proteins of the @entity19 -pre-B-cell line 70Z/3 were separated by @entity214 -PAGE, transferred electrophoretically onto nitrocellulose, and the blot was incubated with @entity558 of the Salmonella minnesota Re-mutant R595 (mRe- @entity558 ). @entity558 bound to proteins on nitrocellulose was immunologically detected by anti-mRe- @entity558 antibodies; @entity558 was associated with one of the membrane proteins of 70Z/3 cells. This protein was 40 kDa under reducing and 45 kDa under non-reducing conditions, respectively. Treatment of 70Z/3 cells with pronase led to the disappearance of the @entity558 -binding protein indicating its surface location. Excess free @entity3275 , which represents the biologically active region of @entity558 , inhibited the binding of mRe- @entity558 to the protein. This @entity558 -binding protein was also identified on the pre-B-cell line CYG8, the B-cell line CYG101 and the @entity19 -T-cell line BW5147. It was, however, not detectable on the B-cell line CYG34 and the @entity1582 -cell line P3-X63-Ag8.653. No other @entity558 -binding protein could be detected on these cell lines. In the @entity19 -macrophage-like cell line J774.1, two @entity558 -binding proteins, one of 40 kDa and one of 80 kDa, were detected. These results indicate that mRe- @entity558 is specifically bound to a 40-kDa protein of lymphocytes, whereas in the case of macrophages it is associated with two @entity558 -binding proteins of 40 and 80 kDa.

Detection of lipopolysaccharide-binding proteins on membranes of XXXX lymphocyte and macrophage-like cell lines.

@entity3275 :: ('MESH:D008050', 'Chemical') :: ['lipid A'] @entity558 :: ('MESH:C536528', 'Disease') :: ['LPS'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity1582 :: ('MESH:D009101', 'Disease') :: ['myeloma'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS']
@entity19 :: (10090,Species) :: ['murine']
