UNASSIGNED: Increasing results suggest microRNAs (miRNAs) could function important roles in @entity5 progression. miR-30a-5p is downregulated in variety of @entity5 and acts as a @entity5 suppressing gene. The functions and molecular mechanisms of miRNA-30a-5p in @entity157 ( @entity157 ) remain unclear. In the present study, quantitative real-time PCR (qRT-PCR) was used to detect miR-30a-5p expression in 16 pairs of @entity157 and their adjacent non-cancerous tissues and @entity157 cell lines. By overexpression of miRNA-30a-5p, CCK-8 and colon formation assay were used to evaluate cell growth and flow cytometry to evaluate cell apoptosis. Western blot was used to test protein expression. And potential mechanisms were analyzed with luciferase activity assay. In vivo @entity5 growth was observed with @entity19 . Our results showed that miR-30a-5p expression in @entity157 tissues was significantly lower compared to adjacent non-cancerous liver tissues, and lower miR-30a-5p expression was also observed in @entity157 cell lines compared to normal liver cell. Luciferase assay showed that @entity60789 ( @entity60789 ) mRNA was a direct target of miR-30a-5p. A significant reverse correlation between miR-30a-5p and @entity60789 in @entity96 tissues was observed. miR-30a-5p overexpression in @entity157 cells significantly inhibited cell proliferation, colon formation, and induced apoptosis while @entity60789 overexpression reversed growth inhibition and apoptosis induction of miRNA-30a-5p in @entity157 cells. miRNA-30a-5p upregulated phosphatase and tensin homolog ( @entity2370 ) protein expression and thus inhibited @entity1994 activating by targeting @entity60789 . miRNA-30a-5p also significantly inhibited @entity5 growth in vivo. Our results suggest that underexpression of miR-30a-5p might function as a @entity5 suppressing miRNA by directly targeting @entity60789 in @entity157 and is therefore a potential candidate biomarker for @entity157 targeting therapy.

Overexpression of microRNA-30a-5p inhibits @entity96 cell proliferation and induces apoptosis by targeting @entity60789 / @entity2370 / XXXX pathway.

@entity96 :: ('MESH:D008113', 'Disease') :: ['liver cancer'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancers', 'cancer', 'malignant tumor', 'HepG2 tumor'] @entity19 :: ('10090', 'Species') :: ['nude mice'] @entity2370 :: ('19211(Tax:10090)', 'Gene') :: ['PTEN'] @entity60789 :: ('67154(Tax:10090)', 'Gene') :: ['metadherin', 'MTDH'] @entity1994 :: ('11651(Tax:10090)', 'Gene') :: ['AKT'] @entity157 :: ('MESH:D006528', 'Disease') :: ['HCC', 'hepatocellular carcinoma']
@entity1994 :: (11651(Tax:10090),Gene) :: ['AKT']
