This study used a co-culture system with Transwell tissue-culture inserts to investigate the role of primary cultures of @entity35 peritoneal mesothelial cells on the proliferation of @entity35 @entity694 cells (CC531 cells). Mesothelial cells significantly inhibited the growth of CC531 cells, while, conversely, CC531 cells stimulated the growth of mesothelial cells. Receptor-binding studies demonstrated the presence of high-affinity @entity2135 receptors on the mesothelial and CC531 cells. Both cell types also produced @entity2135 , as measured by radioimmunoassay. @entity2135 stimulated DNA synthesis in mesothelial cells, but had no effect on the growth of CC531 cells. In co-culture, it was found that @entity2135 potentiated the inhibitory effect of mesothelial cells on CC531 cells. The effect of @entity2135 on mesothelial-cell proliferation was additive to the stimulatory effect of CC531 cells. @entity1810 had no effect on the growth of the CC531 cells, suggesting that this @entity3247 is not involved in the inhibitory effect of mesothelial cells on CC531 cell growth. The study provides evidence for the existence of a paracrine loop between mesothelial and @entity694 cells, giving more insight into the basic cellular mechanisms that may modulate the growth of intraperitoneal @entity694 . Inhibition of CC531-cell proliferation by @entity35 mesothelial cells might explain the earlier finding that @entity5 cells grow poorly in a surgically uncompromised abdomen.

Paracrine interactions between mesothelial and @entity694 cells in a XXXX model.

@entity5 :: ('MESH:D009369', 'Disease') :: ['tumour'] @entity3247 :: ('117038(Tax:10116)', 'Gene') :: ['growth (-inhibitory) factor'] @entity2135 :: ('24482(Tax:10116)', 'Gene') :: ['IGF-I'] @entity1810 :: ('59086(Tax:10116)', 'Gene') :: ['TGF-beta'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon-carcinoma', 'colon carcinoma']
@entity35 :: (10116,Species) :: ['rat']
