@entity2926 is a worldwide zoonotic disease caused by pathogenic Leptospira, a genus of which more than 250 serovars have been identified. Commercial bacterin vaccines are limited in that they lack both cross-protection against heterologous serovars and long-term protection. This study investigated in @entity19 the immunogenicity of an anti- @entity2926 vaccine using the outer membrane proteins LipL32 and Loa22 as antigens. The immunogenicity of this vaccine formulation was compared to those induced by vaccines based on LipL32 or Loa22 alone. A DNA-encapsulated chitosan nanoparticle was used for in vivo DNA delivery. Using a unique DNA plasmid expressing both lipL32 and loa22 for vaccination, higher antibody responses were induced than when combining plasmids harboring each gene separately. Therefore, this formulation was used to test the immunogenicity when administered by a heterologous prime (DNA)-boost (protein) immunization regimen. The specific antibody responses against LipL32 (total IgG and @entity9686 ) and Loa22 ( @entity9686 ) were higher in @entity19 receiving two antigens in combination than those vaccinated with a single antigen alone. Although no significant difference in splenic @entity1961 (+) T cell proliferation was observed among all groups of vaccinated @entity19 , splenocytes from @entity19 vaccinated with two antigens exhibited higher interferon-y and @entity2047 production than when using single antigens alone upon in vitro restimulation. Taken together, the immunogenicity induced by LipL32 and Loa22 antigens in a heterologous prime-boost immunization regimen using chitosan as a DNA delivery system induces higher immune response, and may be useful for developing a better vaccine for @entity2926 .

Immunogenicity of a DNA and Recombinant Protein Vaccine Combining LipL32 and Loa22 for XXXX Using Chitosan as a Delivery System.

@entity2926 :: ('MESH:D007922', 'Disease') :: ['leptospirosis', 'Leptospirosis'] @entity2047 :: ('16183(Tax:10090)', 'Gene') :: ['IL-2'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity9686 :: ('16017(Tax:10090)', 'Gene') :: ['IgG1'] @entity1961 :: ('12504(Tax:10090)', 'Gene') :: ['CD4']
@entity2926 :: (MESH:D007922,Disease) :: ['Leptospirosis']
