BACKGROUND _ AIMS: Angiogenesis, formation of new capillary blood vessels, is crucial for gastroduodenal @entity1066 because it enables delivery of @entity26 and nutrients to the healing site. Because angiogenesis is stimulated by @entity7 ( @entity7 ) and @entity3658 ( @entity3658 ), we studied whether local gene therapy with nonviral DNA encoding @entity7 and/or @entity3658 into the @entity1066 base could accelerate @entity1066 through enhanced angiogenesis. METHODS: Gastric ulcers were induced in @entity35 by @entity1543 applied to the serosal surface of the stomach, and the site around the @entity1066 was injected with nonviral plasmid-encoding full-length complementary DNA (cDNA) of @entity1 recombinant (rh) @entity1655 , rhAng1, or their combination. For some studies, neutralizing anti- @entity7 antibody was administered. RESULTS: Single local injection of plasmids encoding @entity1655 and @entity3658 significantly increased @entity2295 and accelerated @entity1066 . A neutralizing anti- @entity7 antibody significantly reduced the acceleration of @entity1066 resulting from the treatment. Coinjection of both plasmids encoding rhVEGF165 and rhAng1 resulted in formation of more mature vessels and to more complete restoration of gastric glandular structures within the @entity1066 . However, this did not result in further reduction of @entity1066 size. CONCLUSIONS: @entity7 and @entity3658 gene therapy, with limited duration of target gene expression, significantly accelerates gastric @entity1066 . Coinjection of both plasmids leads to more complete structural restoration. Inhibition of accelerated healing by a neutralizing anti- @entity7 antibody indicates an essential role for @entity7 and enhanced angiogenesis in @entity1066 .

Gene therapy for gastric ulcers with single local injection of naked DNA encoding XXXX and @entity3658 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity7 :: ('7422', 'Gene') :: ['vascular endothelial growth factor', 'VEGF'] @entity3658 :: ('284', 'Gene') :: ['Ang1', 'angiopoietin-1'] @entity1066 :: ('MESH:D014456', 'Disease') :: ['ulcer', 'ulcer scar', 'ulcer healing'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity2295 :: ('MESH:D015861', 'Disease') :: ['neovascularization'] @entity1655 :: ('MESH:C467484', 'Chemical') :: ['VEGF165'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity1543 :: ('MESH:D019342', 'Chemical') :: ['acetic acid']
@entity7 :: (7422,Gene) :: ['VEGF']
