OBJECT: The glycoprotein molecule sonic hedgehog ( @entity13827 ) has been shown to play a critical role in neuraxial development. To assess its role in the repair of @entity17 following @entity1566 ( @entity1566 ), escalating doses of @entity13827 were injected into @entity17 in adult @entity35 spinal cords. METHODS: Twenty-seven adult @entity35 underwent thoracic laminectomy and chemical @entity17 of the spinal cord dorsal columns without @entity51 A subset of 20 @entity35 was treated after 3 days by direct injection of @entity13827 at two different doses. @entity35 were killed at 7 or 21 days after @entity1566 , and tissue samples underwent immediate fixation or were placed into cell culture. Diffuse cellular proliferative responses throughout the gray and white matter were observed in up to 70% of @entity13827 -treated @entity35 . Proliferation around the central canal, believed to be derived from the @entity67 consistent with neuronal stem cell induction, was demonstrated in up to 60% of the treated @entity35 . No significant proliferation in these areas was detected in control @entity35 . Dorsal areas of nestin-positive cells were also observed in 70% of @entity35 treated with high doses of @entity13827 , and these observations were reproduced in cell culture as well as in cultures of dorsal spinal cord explants. Cell counts revealed significant increases in the percentage of oligodendrocyte precursors and neurons in treated compared with control @entity35 . CONCLUSIONS: Exogenous @entity13827 administration promotes nestin-positive cell proliferation after @entity1566 in adult rodents. These cells are believed to be derived from neural precursor cells. The populations of oligodendrocyte precursors and neurons were likewise increased in @entity13827 -treated @entity35 , suggesting that these cells may be derived from neural stem cells.

Sonic hedgehog-induced neural precursor proliferation after adult rodent XXXX .

@entity17 :: ('MESH:D003711', 'Disease') :: ['demyelination', 'demyelinated lesions'] @entity35 :: ('10116', 'Species') :: ['rat', 'Rats', 'rats'] @entity13827 :: ('29499(Tax:10116)', 'Gene') :: ['Shh'] @entity51 :: ('MESH:D009461', 'Disease') :: ['neurological deficit'] @entity1566 :: ('MESH:D013119', 'Disease') :: ['spinal cord injury', 'SCI'] @entity67 :: ('MESH:D018754', 'Disease') :: ['ventricular ependyma']
@entity1566 :: (MESH:D013119,Disease) :: ['spinal cord injury']
