@entity14841 is a potent inhibitor of regeneration following spinal @entity1566 . To develop a better understanding of the mechanisms responsible for regenerative failure we used a yeast two-hybrid approach to try and identify proteins that interact with @entity14841 . We identified a novel mitochondrial protein designated @entity69213 ( @entity69213 ) in a screen of an adult @entity1 brain cDNA library. This interaction was confirmed by co-immunoprecipitation in both brain tissue (endogenous) and transfected HEK293T cells (overexpressed). In support of these studies we demonstrate that @entity14841 interacts with the @entity69214 and @entity56944 components of complex III, within the mitochondrial respiratory chain. The mitochondrial localization of @entity69213 was evidenced by confocal image analysis and western blot analysis of isolated mitochondria. @entity69213 is highly conserved and ubiquitously expressed in mitochondria-enriched tissues. Within the CNS, @entity69213 -like immunoreactivity is present in neurons and astrocytes. These data suggest that @entity69213 is a novel mitochondrial protein that interacts with @entity14841 . The interaction of @entity14841 with mitochondrial proteins may provide insight into the mechanisms for @entity14841 -induced inhibition of neurite growth.

Identification and characterization of a novel XXXX ( @entity69213 ).

@entity1 :: ('9606', 'Species') :: ['human'] @entity69214 :: ('7384', 'Gene') :: ['UQCRC1'] @entity69213 :: ('84816', 'Gene') :: ['NIMP', 'Nogo-interacting mitochondrial protein'] @entity56944 :: ('7385', 'Gene') :: ['UQCRC2'] @entity14841 :: ('57142', 'Gene') :: ['Nogo'] @entity1566 :: ('MESH:D013119', 'Disease') :: ['cord injury']
@entity69213 :: (84816,Gene) :: ['Nogo-interacting mitochondrial protein']
