@entity8315 (JCV) is a common @entity1 polyomavirus that infects 70-80% of the population worldwide. In immunosuppressed individuals, JCV infects oligodendrocytes and causes a fatal @entity17 known as @entity7578 ( @entity7578 ). The tropism of JCV is restricted to oligodendrocytes, astrocytes, and B lymphocytes. Several mechanisms may contribute to the restricted tropism of JCV, including the presence or absence of cell-type-specific transcription and replication factors and the presence or absence of cell-type-specific receptors. We have established a system to investigate cellular factors that influence viral tropism by selecting JCV-resistant cells from a susceptible glial cell line (SVG-A). SVG-A cells were subjected to several rounds of @entity1800 using @entity8315 ( @entity25089 ). A population of resistant cells emerged (SVGR2) that were refractory to infection with the @entity66857 strain of JCV, the hybrid virus @entity25089 , as well as to the related polyomavirus SV40. SVGR2 cells were as susceptible as the SVG-A cells to infection with an unrelated amphotropic retrovirus. The stage at which these cells are resistant to infection was investigated and the block appears to be at early viral gene transcription. This system should ultimately allow us to identify glial specific factors that influence the tropism of JCV.

Derivation of a XXXX -resistant @entity1 glial cell line: implications for the identification of host cell factors that determine viral tropism.

@entity1 :: ('9606', 'Species') :: ['human'] @entity17 :: ('MESH:D003711', 'Disease') :: ['demyelinating disease'] @entity66857 :: ('10608', 'Gene') :: ['Mad-4'] @entity8315 :: ('10632', 'Species') :: ['JC virus'] @entity1800 :: ('MESH:D014777', 'Disease') :: ['viral infection'] @entity25089 :: ('100507027', 'Gene') :: ['M1/SVE Delta'] @entity7578 :: ('MESH:D007968', 'Disease') :: ['progressive multifocal leukoencephalopathy', 'PML']
@entity8315 :: (10632,Species) :: ['JC virus']
