The extent of dissociation of various hemoglobins into subunits was estimated from their elution volumes (V(e)) on G-100 Sephadex. Under the same controlled conditions carboxyhemoglobins A, A3 (A(1)), F, S, and C all had the same elution volumes. The @entity1473 and cyanmet derivatives of hemoglobin Kansas (a variant with very low @entity26 affinity) had a relatively high V(e), indicating a decreased mean molecular weight and therefore an increased tendency to form dimers and even monomers. Conversely, the liganded derivatives of hemoglobin Chesapeake (a variant with high @entity26 affinity) had a relatively low V(e), suggestive of an @entity73 . Deoxyhemoglobin Chesapeake had a V(e) identical with that of deoxyhemoglobin A. Cat hemoglobin, known to have an unusually low @entity26 affinity, was found to have a higher V(e) than @entity1 , @entity235 , @entity95 , @entity35 , or @entity840 hemoglobins. Haptoglobin is thought to bind alphabeta dimers in preference to the alpha(2)beta(2)-tetramer. The comparative haptoglobin affinities of the @entity1 hemoglobins were measured by competition between the test hemoglobin and radioactive reference hemoglobin for haptoglobin binding sites. Hemoglobins A, F, S, and C all seemed to bind equally readily, but hemoglobin Kansas and cat hemoglobin showed a higher affinity, and hemoglobin Chesapeake a lower affinity. These results are in accord with recently proposed models which predict that hemoglobins which have an increased degree of @entity4785 will have a low @entity26 affinity, and vice versa.

Subunit dissociation of certain abnormal XXXX hemoglobins.

@entity1 :: ('9606', 'Species') :: ['human'] @entity235 :: ('9615', 'Species') :: ['dog'] @entity73 :: ('MESH:D003072', 'Disease') :: ['impaired degree of subunit dissociation'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity840 :: ('10141', 'Species') :: ['guinea pig'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity4785 :: ('MESH:D004213', 'Disease') :: ['subunit dissociation'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity1473 :: ('CHEBI:46883', 'Chemical') :: ['carboxy']
@entity1 :: (9606,Species) :: ['human']
