It has been suggested that gains of chromosomes 7 and 17 and loss of Y occur in @entity652 and that progression to @entity1358 is marked by gains of additional chromosomes, most frequently 12, 16, and 20. Previous studies have included very few lesions of <5 mm in diameter, a requirement of the present definition of @entity652 . Ten @entity652 (ranging from 1 to 5 mm in diameter) from autopsy material and 10 surgically resected @entity1358 were studied with fluorescence in situ hybridization in paraffin sections using centromeric probes for chromosomes 7, 12, 16, 17, 20, and Y diluted 1:100 with tDenHyb1 buffer. The signals in 50 to 150 nuclei were counted in each @entity5 . Controls for all the probes were normal renal tissues from the same @entity1 . Three or more signals per nucleus were frequently observed in @entity652 : chromosome 7 (range, 10 to 50%; > or = 30% in 9 of 10), 17 (range, 10 to 47%; > or = 30% in 7), 16 (range, 1 to 63%; > or = 10% in 5), 12 (range, 0 to 32%; > or =10% in 4), and 20 (range, 5 to 49%; > or = 10% in 5). Loss of the Y chromosome was observed in 80 to 90% of nuclei in 9 @entity652 from males. Three or more signals were frequent in @entity1358 : chromosome 7 (range, 32 to 63%; > or =30% in 10 of 10), 17 (range, 28 to 61%; > or = 30% in 7), 16 (range, 0 to 45%; > or = 10% in 6), 12 (range, 1 to 37, > or = 10% in 5), 20 (range, 2 to 44%; > or = 10% in 4). No signal for Y was observed in 12 to 88% (> or = 81% in 6) of nuclei in 7 @entity189 from males. Statistical analysis showed no difference between @entity652 and @entity189 . Gains of chromosomes 7, 17, 16, 12, and 20 and loss of the Y chromosome occur early in the evolution of @entity1358 in @entity5 that are only a few millimeters in diameter. Progressive gains of these chromosomes do not appear to correlate with the transition from @entity2513 .

Gains of chromosomes 7, 17, 12, 16, and 20 and loss of Y occur early in the evolution of XXXX : a fluorescent in situ hybridization study.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinomas'] @entity1358 :: ('MESH:D002292', 'Disease') :: ['papillary renal cell carcinoma', 'papillary renal cell carcinomas', 'papillary renal cell neoplasia'] @entity652 :: ('MESH:D000236', 'Disease') :: ['renal papillary adenoma', 'papillary adenoma', 'adenomas', 'papillary adenomas'] @entity2513 :: ('MESH:C563365', 'Disease') :: ['adenoma to carcinoma']
@entity1358 :: (MESH:D002292,Disease) :: ['papillary renal cell neoplasia']
