AIM: We optimized a rapid and efficient tissue lysis method using the MagNA Lyser (Roche, Germany). Using this novel method combined with immunoblot analysis, we investigated the correlation between abnormal @entity1836 expression and clinicopathological characteristics in @entity14 . METHODS: Tissue samples from @entity35 were tested to determine optimal lysis conditions for use with MagNA Lyser. We next used the new method to extract tissue proteins from the @entity5 tissue of a @entity14 @entity1 . The availability of extractable tissue proteins for proteomic study was demonstrated by two-dimensional (2D) gel electrophoresis and subsequent matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. In addition, we prepared tissue lysates from paired @entity5 tissues and adjacent nontumor tissues of 50 @entity14 @entity1 . Ensuing immunoblot analyses were performed to detect the level of @entity1836 expression. RESULTS: The optimal sample sizes processed were found to be around 200 mg, with oscillation frequency of 6500 r/min for 80 s. Test of the first @entity1 tissue lysate confirmed that the MagNA Lyser method was adequate for protein extraction and subsequent identification by current proteomic protocols. The method was also applicable to immunoblot analysis. Thirty of 50 (60%) colorectal @entity1 exhibited higher level of @entity1836 expression in their @entity5 tissues. Raised level of @entity1836 expression correlated with @entity1 ' gender and @entity5 cell proliferation index (P = 0.037 and P<0.001, respectively), but was independent of clinicopathological characteristics and overall survival. CONCLUSION: We report a novel tissue lysis method applicable to proteomic and immunoblot analyses, which can facilitate the discovery and detection of @entity5 protein alterations.

Novel rapid tissue lysis method to evaluate @entity5 proteins: correlation between elevated XXXX expression and @entity14 cell proliferation.

@entity1 :: ('9606', 'Species') :: ['human', 'patients', 'patient'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity1836 :: ('598', 'Gene') :: ['Bcl-X(L)'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal carcinoma', 'colorectal cancer'] @entity35 :: ('10116', 'Species') :: ['Sprague-Dawley rats']
@entity1836 :: (598,Gene) :: ['Bcl-X(L)']
