A pro-inflammatory cytokine mixture (CM: @entity3426 , @entity5 @entity39 factor-a and interferon-y) and @entity3426 -induced @entity16309 activity have been shown to increase the proliferation of @entity35 dental pulp cells and @entity19 stem cell-derived odontoblast-like cells. This suggests that @entity1549 may regulate wound healing and regeneration in the odontoblast-rich dental pulp. Here, we determined whether these results can be extrapolated to @entity1 dental pulp by investigating the effects of CM-induced @entity1549 up-regulation on the proliferation and apoptosis of purified odontoblast-like cells derived from @entity1 skeletal muscle stem cells. We used siRNA to specifically reduce @entity1549 expression. We found that CM treatment increased @entity1549 mRNA and protein levels as well as @entity1549 activity. Cell proliferation was also markedly increased, with no changes in apoptosis, upon treatment with CM and following the application of exogenous @entity1549 . Endogenous tissue inhibitors of metalloproteinases were constitutively expressed during all experiments and unaffected by @entity1549 . Although treatment with @entity1549 siRNA suppressed cell proliferation, it also unexpectedly increased apoptosis. This siRNA-mediated increase in apoptosis could be reversed by exogenous @entity1549 . These results demonstrate that cytokine-induced @entity1549 activity regulates cell proliferation and suppresses apoptosis in @entity1 odontoblast-like cells.

Unique proliferation response in odontoblastic cells derived from XXXX skeletal muscle stem cells by cytokine-induced matrix metalloproteinase-3.

@entity1 :: ('9606', 'Species') :: ['human'] @entity39 :: ('MESH:D009336', 'Disease') :: ['necrosis'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity19 :: ('10090', 'Species') :: ['murine'] @entity1549 :: ('4314', 'Gene') :: ['MMP-3'] @entity16309 :: ('171045(Tax:10116)', 'Gene') :: ['matrix metalloproteinase (MMP)-3'] @entity3426 :: ('24494(Tax:10116)', 'Gene') :: ['IL-1b', 'interleukin (IL)-1b'] @entity35 :: ('10116', 'Species') :: ['rat']
@entity1 :: (9606,Species) :: ['human']
