The @entity69787 antigen is an early and specific marker of eosinophil differentiation in the @entity1573 haematopoietic system. To elucidate the transciptional events controlling commitment to the eosinophil lineage, we studied the regulation of the eosinophil-specific @entity69787 promoter. This promoter is TATA-less, and binds trancription factors of the Ets, C/EBP, GATA and @entity69788 families. These sites are contained within a 309 bp promoter fragment which is sufficient for specific high level transcription in an eosinophil cell line. Co-transfection experiments in Q2bn fibroblasts showed cooperative activation of the @entity69787 proximal promoter by @entity69788 , @entity69789 / @entity69790 , @entity59799 and @entity69791 . The @entity69789 / @entity69790 and @entity69791 proteins were the most potent activators, and acted with high synergy through juxtaposed binding sites located approximately 60 bp upstream of the transcription start site. The @entity69789 and @entity69791 proteins were found to associate physically via their DNA-binding domains and to bind their combined binding site cooperatively. @entity59799 showed biphasic regulation of the @entity69787 promoter, activating at low and repressing at high protein concentrations. These results demonstrate combinatorial activation of an eosinophil-specific promoter by ubiquitous and lineage-restricted haematopoietic transcription factors. They also indicate that direct interactions between C/EBPs and specific Ets family members, together with @entity59799 , are important for eosinophil lineage determination.

Regulation of eosinophil-specific gene expression by a C/EBP-Ets complex and XXXX .

@entity69789 :: ('396235(Tax:9031)', 'Gene') :: ['Ets-1'] @entity1573 :: ('9031', 'Species') :: ['chicken'] @entity69787 :: ('396127(Tax:9031)', 'Gene') :: ['EOS47'] @entity69788 :: ('396244(Tax:9031)', 'Gene') :: ['Myb', 'c-Myb'] @entity69791 :: ('427549(Tax:9031)', 'Gene') :: ['C/EBPalpha'] @entity59799 :: ('396450(Tax:9031)', 'Gene') :: ['GATA-1'] @entity69790 :: ('419723(Tax:9031)', 'Gene') :: ['Fli-1']
@entity59799 :: (396450(Tax:9031),Gene) :: ['GATA-1']
