Acetylcholinesterase (AChE) is the target of @entity5139 (OP) and @entity2961 insecticides. Mutations in the AChE gene (ace) leading to decreased insecticide susceptibility is the main resistance mechanism in insects. In this study, two Chilo auricilius acetylcholinesterase genes, designated as Caace1 and Caace2, were cloned using RT-PCR and RACE. Caace1 cDNA is 2534   bp, with ORF of 2082   bp, and it encodes an acetylcholinesterase 1 (CaAChE1) protein comprising a calculated 693 @entity3689 (aa) residues. Caace2 cDNA contains 2280   bp, with a full-length ORF of 1917   bp, encoding acetylcholinesterase 2 (CaAChE2) comprising a calculated 638 aa residues. At the aa level, CaAChE1 displays the highest similarity (97%) with the @entity42683 AChE1, and CaAChE2 shows the highest similarity with the @entity42683 AChE2 (99%). From the restriction fragment length polymorphism (RFLP) PCR (RFLP-PCR) analysis, one mutation in Caace1, similar to the ace1 mutation associated with @entity68071 resistance in @entity42683 , was detected. Detailed examination of field populations of C. auricilius indicated this resistance mutation in C. auricilius is still quite infrequent. Based on the assay of AChE activity and RFLP-PCR testing, an individual that contains resistance mutation has lower AChE activities, while the individual that does not contain the resistance mutation has higher AChE activities. This study provides a basis for future investigations into the mechanism of OP resistance in C. auricilius, as well as a guidance for C. auricilius control with reasonable choice of pesticides.

Cloning of Two Acetylcholinesterase Genes and Analysis of Point Mutations Putatively Associated with XXXX Resistance in Chilo auricilius (Lepidoptera: Pyralidae).

@entity42683 :: ('168631', 'Species') :: ['C. suppressalis', 'Chilo suppressalis'] @entity5139 :: ('D010755', 'Chemical') :: ['organophosphate'] @entity2961 :: ('CHEBI:28616', 'Chemical') :: ['carbamate'] @entity3689 :: ('D000596', 'Chemical') :: ['amino acid'] @entity68071 :: ('MESH:C012584', 'Chemical') :: ['triazophos']
@entity68071 :: (MESH:C012584,Chemical) :: ['Triazophos']
