DNA amplifications are important mechanisms for proto-oncogene activation. Comparative genomic hybridization (CGH) to metaphase chromosome preparations has revealed amplifications in 10-20% of @entity1892 ( @entity1893 ). We analysed a series of 16 aggressive @entity1893 by the new approach termed Matrix-CGH (M-CGH) using genomic DNA microarrays as hybridization target. For M-CGH, a dedicated @entity1892 chip was constructed containing 496 genomic targets covering oncogenes, @entity5 suppressor genes as well as chromosome regions frequently altered in @entity1893 . In 10 of 16 samples a total of 15 DNA amplifications were identified. The amplicons included @entity588 , @entity4632 , @entity4910 , @entity17946 , @entity4016 , @entity10008 and @entity7465 . Four of the 15 amplifications remained undetected by chromosomal CGH. The respective amplicons mapped to bands 2p13, 9p13-p21 and 12q24 and, were confirmed by fluorescence in situ hybridization. Furthermore, for four genomically amplified genes real-time quantitative reverse transcription polymerase chain reaction revealed elevated mRNA expression levels. These data show the superior diagnostic sensitivity of the newly developed diagnostic tool. As only a small portion of the genome (approximately 1.5%) has been analysed by the present DNA array, it is likely that gene amplifications are much more common in @entity146 than previously assumed.

Hidden gene amplifications in XXXX detected by microarray-based comparative genomic hybridization.

@entity588 :: ('596', 'Gene') :: ['BCL2'] @entity146 :: ('MESH:D001523', 'Disease') :: ['aggressive lymphomas'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity7465 :: ('4193', 'Gene') :: ['MDM2'] @entity17946 :: ('894', 'Gene') :: ['CCND2'] @entity4910 :: ('595', 'Gene') :: ['CCND1'] @entity4016 :: ('3717', 'Gene') :: ['JAK2'] @entity1892 :: ('MESH:D016393', 'Disease') :: ['B-cell lymphomas', 'B-cell lymphoma'] @entity1893 :: ('MESH:D008228', 'Disease') :: ['non-Hodgkin lymphomas', 'B-NHL'] @entity10008 :: ('2249', 'Gene') :: ['FGF4'] @entity4632 :: ('5966', 'Gene') :: ['REL']
@entity1892 :: (MESH:D016393,Disease) :: ['aggressive B-cell non-Hodgkin lymphomas']
