BACKGROUND _ AIMS: The role of amidated gastrin17 (G17) and the @entity8243 / @entity30247 / @entity30247 in @entity14 is still a controversial issue. Here, we investigated the effect of G17 on proliferation and apoptosis of @entity30247 -expressing @entity1 @entity694 cell lines in vitro and in vivo. METHODS: Proliferation was determined by cell counting and cell cycle analysis. Apoptosis was analyzed by annexin V staining, TUNEL staining, @entity522 /7 assay, and JC1 (delta psi) assay. Signal-transduction pathways were analyzed by Western blotting and gel-shift and luciferase assays. An in vivo @entity5 model with subcutaneously inoculated @entity694 cells in @entity466 @entity19 was used, and systemic hypergastrinemia was induced by @entity4546 . RESULTS: In @entity1 cells stably transfected with the wild-type @entity30247 ( @entity1 wt) or in Lovo cells endogenously expressing CCK2 receptors, G17 treatment inhibited proliferation along with a G2/M cell cycle arrest. Furthermore, the administration of G17 significantly augmented apoptosis of @entity30247 -expressing cells. In contrast, G17 had no effect on proliferation and apoptosis in @entity1 cells stably transfected with a @entity5 -derived @entity30247 mutant ( @entity1 mut) or in cells lacking @entity30247 expression. Systemic hypergastrinemia in @entity466 ( @entity466 ) @entity19 suppressed the growth of Colo320wt @entity5 accompanied by enhanced apoptosis as compared with untreated @entity5 . In contrast, @entity4546 did not affect Colo320mut @entity5 reflecting a loss-of-function state of the CCK2(mut) receptor. This is supported by the observation that, in @entity1 wt cells, but not in @entity1 mut cells, G17 treatment induced the MAPK/ERK/AP-1 pathway and inhibited the activity of NF-kappaB. CONCLUSIONS: G17 exerts an antiproliferative and proapoptotic effect on @entity1 @entity694 cells expressing the wild-type @entity30247 . This supports the view that amidated @entity8243 prevents rather than promotes @entity14 .

@entity8243 suppresses growth of XXXX expressing @entity694 cells by inducing apoptosis in vitro and in vivo.

@entity1 :: ('9606', 'Species') :: ['human', 'Colo320'] @entity522 :: ('836', 'Gene') :: ['caspase-3'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity30247 :: ('887', 'Gene') :: ['CCKB', 'CCK2 receptor'] @entity8243 :: ('2520', 'Gene') :: ['gastrin'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal carcinogenesis'] @entity694 :: ('MESH:D003110', 'Disease') :: ['colon cancer'] @entity4546 :: ('MESH:D009853', 'Chemical') :: ['omeprazole'] @entity466 :: ('MESH:D016511', 'Disease') :: ['SCID', 'severe combined immunodeficiency']
@entity30247 :: (887,Gene) :: ['CCK2 receptor']
