OBJECTIVE: To explore the apoptosis inducing effects of @entity4447 ( @entity9188 ) on @entity1 @entity150 cells and elucidate possible mechanisms. METHODS: After treatment with @entity9188 , the growth inhibition rates of @entity1 @entity150 cell line BIU-87 were studied by @entity98 and cell counts methods. DNA synthesis rates were detected by 3H-TdR assay. The morphological changes of @entity5 cells were observed by light and electronic microscopy and cell apoptosis rates were detected by @entity10730 -mediated @entity1977 nick end labeling (TUNEL). @entity588 gene expression of BIU-87 cells was observed by strept avidin- @entity1885 complex (SABC) immunohistochemical method. RESULTS: @entity9188 could effectively inhibit the growth of BIU-87 (P < 0.05), which were time and concentration dependent. The inhibition rate of 4.0 mumol/L @entity9188 for DNA synthesis of @entity5 cells was 55.64% (P < 0.01). Partial @entity5 cells presented the characteristic morphological changes of apoptosis which depended on the time of exposure to drug (P < 0.05). @entity588 expression of BIU-87 cells was decreased significantly (P < 0.05). CONCLUSION: @entity9188 can significantly induce apoptosis in @entity150 cells by down-regulating the expression of the @entity588 gene and inhibiting DNA synthesis. This provides a potentially effective method for prevention and cure of @entity1 @entity150 .

Apoptosis inducing effects of @entity4447 on @entity1 XXXX cell line BIU-87.

@entity1 :: ('9606', 'Species') :: ['human'] @entity4447 :: ('MESH:C006632', 'Chemical') :: ['arsenic trioxide'] @entity10730 :: ('1791', 'Gene') :: ['TdT'] @entity588 :: ('596', 'Gene') :: ['bcl-2'] @entity1885 :: ('MESH:D001710', 'Chemical') :: ['biotin'] @entity9188 :: ('CHEBI:30621', 'Chemical') :: ['As2O3'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity1977 :: ('CHEBI:17625', 'Chemical') :: ['dUTP'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity150 :: ('MESH:D001749', 'Disease') :: ['bladder cancer']
@entity150 :: (MESH:D001749,Disease) :: ['bladder cancer']
