A tubular gelatin scaffold for the time-dependent controlled release of epidermal growth factor (EGF) and @entity1138 ( @entity1139 ) was fabricated. EGF was incorporated using silk fibroin carriers, and @entity1139 was planted using @entity1989 ( @entity1989 ) microspheres. The relationship between scaffold properties and crosslinking degrees was evaluated. As the crosslinking degree was increased from 23.7% to 65.3%, the mechanical properties of the scaffold obviously improved, and the compressive modulus increased to approximately 65kPa. The mass degradation of the scaffold was also controlled from 9 days to approximately 1 month. In vitro release tests indicated that the scaffold mainly released EGF in the early period and @entity1139 in the later period. Urethral epithelial cells (UECs) and urethral scar derived fibroblast cells (UFCs) were coseeded in the scaffold at a ratio of 1:1. After 9 days of coculture, immunostaining results displayed that the proportion of UECs continuously increased to approximately 71%. These changes in cell proportion were confirmed by the results of Western blot analysis. Therefore, the scaffold promoted the growth but inhibited the regeneration of UFCs. This scaffold for time-dependent controlled release of multiple biofactors may be potentially useful in urethral reconstruction and other tissue engineering studies.

A tubular gelatin scaffold capable of the time-dependent controlled release of epidermal growth factor and XXXX .

@entity1139 :: ('CHEBI:27504', 'Chemical') :: ['MMC'] @entity1138 :: ('MESH:D016685', 'Chemical') :: ['mitomycin C'] @entity1989 :: ('CHEBI:60159', 'Chemical') :: ['polylactide', 'PLA']
@entity1138 :: (MESH:D016685,Chemical) :: ['mitomycin C']
