@entity2272 ( @entity2271 ) is a plant hormone involved in important processes including development and stress responses. Recent reports have identified a number of plant @entity2272 receptors and transporters, highlighting novel mechanisms of @entity2272 action. In the present paper we describe application of a chemical proteomics approach leading to the identification of mitochondrial ANTs ( @entity1708 translocators) as @entity2272 -interacting proteins. Initial in vitro studies confirmed inhibition of ANT-dependent @entity855 translocation by @entity2272 . Further analysis demonstrated ANT-dependent uptake of @entity2272 into both recombinant @entity958 ANT2-containing proteoliposomes and native isolated @entity2772 mitochondria; the latter with a Km of 3.5   M and a Vmax of 2.5 nmol/min per g of protein. @entity855 was found to inhibit ANT-dependent @entity2272 translocation. Specificity profiles highlight the possibility of mechanistic differences in translocation of @entity2272 and @entity855 . Finally, @entity2272 was shown to stimulate ATPase activity in @entity2772 mitochondrial extracts. @entity2272 concentrations in plant cells are estimated to reach the low micromolar range during stress responses, supporting potential physiological relevance of these in vitro findings. Overall, the present in vitro work suggests the possibility of as yet uncharacterized mechanisms of @entity2272 action in planta related to inhibition of mitochondrial @entity855 translocation and functional localization of @entity2272 in the mitochondrial matrix.

Identification and characterization of interactions between XXXX and mitochondrial @entity1708 translocators.

@entity2272 :: ('CHEBI:2365', 'Chemical') :: ['ABA'] @entity1708 :: ('CHEBI:61293', 'Chemical') :: ['adenine nucleotide'] @entity2271 :: ('MESH:D000040', 'Chemical') :: ['abscisic acid'] @entity2772 :: ('3562', 'Species') :: ['spinach'] @entity958 :: ('3702', 'Species') :: ['Arabidopsis thaliana'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity2271 :: (MESH:D000040,Chemical) :: ['abscisic acid']
