UNASSIGNED: @entity2523 is a @entity2521 -associated pathogen and interactions between the bacterium and gingival fibroblasts play an important role in development and progression of @entity2521 , an @entity32 leading to degeneration of tooth-supporting structures. Gingival fibroblasts, which expresses protease activated receptors (PARs) as well as toll-like receptors (TLRs), produces inflammatory mediators upon bacterial challenges. In this study, we elucidated the importance of PAR1, PAR2, TLR2 and TLR4 for the expression and secretion of CXCL8, interleukin-6 (IL-6), transforming growth factor-b1 (TGF-b1) and secretory leukocyte inhibitor (SLPI). @entity1 gingival fibroblasts were transfected with small-interfering RNA against the target genes, and then stimulated with @entity2523 wild-type W50 and W50-derived double rgp mutant E8 and kgp mutant K1A. TLR2-silencing reduced @entity2523 -induced CXCL8 and IL-6. IL-6 was also reduced after PAR1-silencing. No effects were observed for TGF-b1. SLPI was suppressed by @entity2523 and silencing of PAR1 as well as TLR2, gave additional suppression at the mRNA level. TLR4 was not involved in the regulation of the investigated mediators. CXCL8 and IL-6 are important for progression and development of @entity2521 , leading to a @entity32 that may contribute to the tissue destruction that follows an exacerbated host response. Therefore, regulating the expression of TLR2 and subsequent release of CXCL8 and IL-6 in @entity2521 could attenuate the tissue destruction seen in @entity2521 .

The role of toll-like and protease-activated receptors in the expression of cytokines by gingival fibroblasts stimulated with the periodontal pathogen XXXX .

@entity1 :: ('9606', 'Species') :: ['Human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['chronic inflammation', 'inflammatory disease'] @entity2521 :: ('MESH:D010518', 'Disease') :: ['periodontitis'] @entity2523 :: ('837', 'Species') :: ['Porphyromonas gingivalis', 'P. gingivalis']
@entity2523 :: (837,Species) :: ['Porphyromonas gingivalis']
