@entity19 lymphoid cell cultures are dependent on reducing agents in their culture medium to allow proliferation and survival of the cells. In the case of the @entity19 @entity6689 +-pre-B cell line SPGM-1, withdrawal of @entity6514 (2-ME) resulted in rapid inhibition of proliferation and subsequent cell @entity204 by apoptosis. The pathways leading to cell @entity204 by withdrawal of 2-ME or by incubation with @entity1360 , a known inducer of apoptosis, were compared. Both kinds of stimulation resulted in apoptosis of the whole population, but cell @entity204 occurred with different kinetics. Only apoptosis induced by @entity1360 was inhibited by coincubation with the @entity4621 PMA, while apoptosis induced by withdrawal of 2-ME was not. Overexpression of the @entity1 @entity588 proto-oncogene in these cells delayed the @entity204 process induced by either method. SPGM-1xbcl-2 cells accumulated in the @entity28761 /M cell cycle phases after removal of @entity6514 from the medium, whereas treatment with @entity1360 resulted in an arrest only in the G0/G1 transition. Interestingly, both stimuli induced the expression of the Fas receptor, but with different kinetics, while the @entity9699 ( @entity9699 ) was expressed constitutively in SPGM-1 cells. These data demonstrate that withdrawal of 2-ME and incubation with @entity1360 both induce rapid cell @entity204 by apoptosis, possibly mediated by an autocrine Fas/ @entity9699 loop. Although the initial pathways activated by the two forms of treatment must be different, they converge on a common level controlled by the anti-apoptotic gene product @entity1379 .

Withdrawal of XXXX induces apoptosis in a B-cell line via Fas upregulation.

@entity1 :: ('9606', 'Species') :: ['human'] @entity28761 :: ('53761(Tax:10090)', 'Gene') :: ['G0/G1 and G2'] @entity588 :: ('596', 'Gene') :: ['bcl-2'] @entity19 :: ('10090', 'Species') :: ['Mouse', 'mouse'] @entity4621 :: ('CHEBI:37532', 'Chemical') :: ['phorbol ester'] @entity1360 :: ('MESH:D015759', 'Chemical') :: ['ionomycin'] @entity6514 :: ('MESH:D008623', 'Chemical') :: ['2-ME', '2-mercaptoethanol'] @entity9699 :: ('14103(Tax:10090)', 'Gene') :: ['Fas ligand', 'FasL'] @entity1379 :: ('12043(Tax:10090)', 'Gene') :: ['Bcl-2'] @entity6689 :: ('12507(Tax:10090)', 'Gene') :: ['CD5'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death']
@entity6514 :: (MESH:D008623,Chemical) :: ['2-mercaptoethanol']
