Increased reports demonstrated that @entity22037 , @entity22037 in natural and artificial environments were always suffered from an emerging @entity66 , @entity22037 virus (LMBV). However, the underlying mechanism of LMBV pathogenesis remained largely unknown. Here, we investigated the cell signaling events involved in virus induced cell death and viral replication in  vitro. We found that @entity778 in @entity189 papulosum cyprini (EPC) cells induced typical apoptosis, evidenced by the appearance of apoptotic bodies, cytochrome c release, mitochondrial membrane permeabilization (MMP) destruction and reactive @entity26 species (ROS) generation. Two initiators of apoptosis, caspase-8 and caspase-9, and the executioner of apoptosis, caspase-3, were all significantly activated with the @entity281 time, suggested that not only mitochondrion-mediated, but also death receptor-mediated apoptosis were involved in @entity778 . Reporter gene assay showed that the promoter activity of transcription factors including p53, NF-kB, AP-1 and @entity268 response element-binding protein (CREB) were decreased during @entity778 . After treatment with different signaling pathway inhibitors, virus production were significantly suppressed by the inhibition of @entity4357 3-kinase (PI3K) pathway and extracellular-signal-regulated kinases (ERK) signaling pathway. Furthermore, @entity778 induced apoptosis was enhanced by PI3K inhibitor @entity2306 , but decreased by addition of ERK inhibitor @entity5732 . Therefore, we speculated that apoptosis was sophisticatedly regulated by a series of cell signaling events for efficient virus propagation. Taken together, our results provided new insights into the molecular mechanism of ranavirus @entity281 .

Involvement of the PI3K and ERK signaling pathways in XXXX virus-induced apoptosis and viral replication.

@entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity778 :: ('MESH:D015658', 'Disease') :: ['LMBV infection'] @entity5732 :: ('MESH:C113580', 'Chemical') :: ['UO126'] @entity66 :: ('MESH:D004194', 'Disease') :: ['iridovirus disease'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity189 :: ('MESH:D002277', 'Disease') :: ['epithelioma'] @entity2306 :: ('MESH:C085911', 'Chemical') :: ['LY294002'] @entity22037 :: ('27706', 'Species') :: ['largemouth Bass', 'Micropterus salmoides'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity4357 :: ('MESH:D010716', 'Chemical') :: ['phosphatidylinositol']
@entity22037 :: (27706,Species) :: ['largemouth bass']
