UNASSIGNED: Cigarette smoke (CS)-induced @entity32 is the main pathogenesis of @entity1030 . The present study was designed to evaluate whether ghrelin, a novel @entity5739 , can affect the pro-inflammatory cytokine @entity650 ( @entity650 ) production induced by @entity1981 ( @entity1981 ) in the @entity1 bronchial epithelial cell line (16-HBE) and its possible mechanism. 16-HBE cells were pre-incubated with vehicle or ghrelin (0.1 to 1000  ng/mL) in a concentration-dependent manner, and then @entity1981 (0 to 16  %) was added. The protein levels of @entity650 in the medium were determined by ELISA, and the mRNA expressions of @entity650 was detected by RT-PCR. We also detected the phosphorylation of IKKa/b/ @entity9135 protein and the degradation of inhibitory protein-kB (I-kB) by Western blot analysis. And the generation of reactive @entity26 species (ROS) in 16-HBE was evaluated by labeling specific fluorescence probes @entity21184 . 16-HBE Cells treated with @entity1981 (8  %) exhibited significantly higher @entity650 production compared with cells treated with vehicle alone (P   <   0.05). Ghrelin suppressed @entity1981 -induced @entity650 production at both mRNA and protein levels in a concentration-dependent manner (P   <   0.05). Moreover, ghrelin attenuated @entity1981 -triggered NF-kB activation in 16-HBE, but the intracellular ROS level after application of @entity1981 was not affected by ghrelin (0.1 to 1000  ng/mL). Together, these results suggest that ghrelin inhibits @entity1981 -induced @entity650 production in 16-HBE cells by targeting on NF-kB pathway, but not by scavenging intracellular ROS.

Ghrelin Inhibits XXXX Production Induced by Cigarette Smoke Extract in the Bronchial Epithelial Cell Via NF-kB Pathway.

@entity1 :: ('9606', 'Species') :: ['human'] @entity650 :: ('3569', 'Gene') :: ['IL-6', 'interleukin-6'] @entity32 :: ('MESH:D007249', 'Disease') :: ['airway inflammation'] @entity9135 :: ('5970', 'Gene') :: ['p65'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity21184 :: ('MESH:C029569', 'Chemical') :: ['DCFH-DA'] @entity5739 :: ('51738', 'Gene') :: ['growth hormone-releasing peptide'] @entity1030 :: ('MESH:D029424', 'Disease') :: ['COPD'] @entity1981 :: ('OMIM:188890', 'Disease') :: ['CSE', 'cigarette smoke extract']
@entity650 :: (3569,Gene) :: ['Interleukin-6']
