UNASSIGNED: @entity46469 and its deacetylated metabolite @entity68923 are two major carotenoids that have been confirmed to possess various pharmacological properties. In the present study, @entity68923 was identified as the deacetylated metabolite of @entity46469 , after intravenous (i.v.) and intragastric gavage (i.g.) administration to @entity35 at doses of 2 and 65 mg/kg, respectively, by liquid chromatography-tandem mass spectrometric (LC- @entity15 / @entity15 ) analysis. Next, an accurate and precise LC- @entity15 / @entity15 method was developed to quantitatively determine @entity46469 and @entity68923 in @entity35 plasma. Plasma samples were resolved by LC- @entity15 / @entity15 on a reverse-phase SB-C18 column that was equilibrated and eluted with @entity1626 (A)/aqueous 0.1% @entity5189 (B; 92/8, v/v) at a flow rate of 0.5 mL/min. Analytes were monitored by multiple-reaction monitoring (MRM) under positive electrospray ionization mode. The precursor/product transitions (m/z) were 659.3 > 109.0 for @entity46469 , 617.2 > 109.0 for @entity68923 , and 429.4 > 313.2 for the internal standard (IS). Calibration curves for @entity46469 and @entity68923 were linear over concentrations ranging from 1.53 to 720 and 1.17 to 600 ng/mL, respectively. The inter- and intraday accuracy and precision were within   15%. The method was applied successfully in a pharmacokinetic study and the resulting oral @entity46469 bioavailability calculated.

Simultaneous Determination of XXXX and Its Deacetylated Metabolite @entity68923 in @entity35 Plasma by Liquid Chromatography-Tandem Mass Spectrometry.

@entity46469 :: ('MESH:C025164', 'Chemical') :: ['Fucoxanthin', 'fucoxanthin'] @entity1626 :: ('MESH:C032159', 'Chemical') :: ['acetonitrile'] @entity68923 :: ('MESH:C483031', 'Chemical') :: ['fucoxanthinol'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity15 :: ('MESH:D009103', 'Disease') :: ['MS'] @entity5189 :: ('MESH:C030544', 'Chemical') :: ['formic acid']
@entity46469 :: (MESH:C025164,Chemical) :: ['Fucoxanthin']
