The function of @entity8189 ( @entity6510 ) and @entity52789 ( @entity12332 ) during porcine oocyte maturation was investigated by injecting oocytes with their antisense RNAs (asRNAs). At first, protein levels of both @entity1364 Bs were examined by immunoblotting, revealing that immature oocytes had only @entity12332 , at a level comparable to 1/20 to 1/40 of that detected in first metaphase oocytes. Both @entity1364 B syntheses were started around germinal vesicle breakdown (GVBD); @entity6510 and @entity12332 peaked at the second metaphase and first metaphase, respectively. We obtained a porcine @entity12332 cDNA fragment, which was 88% homologous with @entity1 @entity12332 , by reverse-transcriptase polymerase chain reaction (RT-PCR) using total RNAs of immature porcine oocytes and a primer set of @entity1 @entity12332 . Specific asRNAs of @entity6510 and @entity12332 were prepared in vitro. Then one, the other, or both were injected into the cytoplasm of immature oocytes. @entity6510 asRNA inhibited @entity6510 synthesis specifically; the injected oocytes underwent first meiosis normally but could not arrest at the second meiotic metaphase. @entity12332 asRNA inhibited @entity12332 synthesis specifically, but had almost no effect on the maturation of injected oocytes. When both @entity6510 and @entity12332 asRNAs were injected, synthesis of both @entity1364 Bs was inhibited, and GVBD was significantly suppressed but occurred slowly. These results suggest that @entity6510 is the principal molecule for regulation in @entity1 oocyte maturation, whereas @entity12332 has only an accessory role. They also show that in porcine oocytes, @entity1364 B synthesis is not necessary for GVBD induction itself, but synthesis of at least one @entity1364 B, @entity6510 or @entity12332 , is necessary for GVBD induction in a normal time course.

Analysis of the roles of @entity8189 and XXXX in porcine oocyte maturation by inhibiting synthesis with antisense RNA injection.

@entity1 :: ('9606', 'Species') :: ['mammalian', 'human'] @entity1364 :: ('5111', 'Gene') :: ['cyclin'] @entity6510 :: ('1268', 'Gene') :: ['CB1'] @entity8189 :: ('891', 'Gene') :: ['cyclin B1'] @entity12332 :: ('1269', 'Gene') :: ['CB2'] @entity52789 :: ('9133', 'Gene') :: ['cyclin B2']
@entity52789 :: (9133,Gene) :: ['cyclin B2']
