The respective roles of insulin and @entity1522 in regulation of skeletal muscle protein synthesis and degradation after feeding were examined in @entity35 fasted for 17 h and refed over 1 h with either a 25 or a 0% @entity535 /protein meal. In each nutritional condition, postprandial insulin secretion was either maintained (control groups: C(25) and C(0)) or blocked with @entity6335 injections ( @entity6335 groups: DZ(25) and DZ(0)). Muscle protein metabolism was examined in vitro in epitrochlearis muscles. Only feeding the 25% @entity535 /protein meal in the presence of increased plasma insulin concentration (C(25) group) stimulated protein synthesis and inhibited proteolysis in skeletal muscle compared with the postabsorptive state. The stimulation of protein synthesis was associated with increased phosphorylation of @entity28888 ( @entity28888 ), reduced binding of @entity3801 to @entity28888 , and increased assembly of the active @entity3801 . @entity44031 complex. The p70 S6 kinase (p70( @entity14446 )) was also hyperphosphorylated in response to the 25% @entity535 /protein meal. Acute @entity4053 induced by @entity6335 injections totally abolished these effects. Feeding the 0% @entity535 /protein meal with or without @entity4053 did not stimulate muscle protein synthesis, reduce proteolysis, or regulate initiation factors and p70( @entity14446 ) compared with fasted @entity35 . Taken together, our results suggest that both insulin and @entity1522 are required to stimulate protein synthesis, inhibit protein degradation, and regulate the interactions between @entity3801 and @entity28888 or @entity44031 in response to feeding.

XXXX and insulin are both required to regulate assembly of the @entity3801 . @entity44031 complex in @entity35 skeletal muscle.

@entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity28888 :: ('116636(Tax:10116)', 'Gene') :: ['eukaryotic initiation factor (eIF)4E binding protein-1', '4E-BP1'] @entity6335 :: ('MESH:D003981', 'Chemical') :: ['diazoxide'] @entity3801 :: ('117045(Tax:10116)', 'Gene') :: ['eIF4E'] @entity4053 :: ('MESH:D007003', 'Disease') :: ['postprandial insulin deficiency'] @entity44031 :: ('287986(Tax:10116)', 'Gene') :: ['eIF4G'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity14446 :: ('83840(Tax:10116)', 'Gene') :: ['S6k']
@entity1522 :: (CHEBI:33709,Chemical) :: ['Amino acids']
