UNASSIGNED: Adult mesenchymal stromal cell-based interventions have shown promising results in a broad range of diseases. However, their use has faced limited effectiveness owing to the low survival rates and susceptibility to environmental stress on transplantation. We describe the cellular and molecular characteristics of multilineage-differentiating stress-enduring (Muse) cells derived from @entity1386 ( @entity1386 ), a subpopulation of pluripotent stem cells isolated from @entity1 lipoaspirates. Muse- @entity1386 cells were efficiently obtained using a simple, fast, and affordable procedure, avoiding cell sorting and genetic manipulation methods. Muse- @entity1386 cells isolated under severe cellular stress, expressed pluripotency stem cell markers and spontaneously differentiated into the three germ lineages. Muse- @entity1386 cells grown as spheroids have a limited proliferation rate, a diameter of    15   m, and ultrastructural organization similar to that of embryonic stem cells. Muse- @entity1386 cells evidenced high stage-specific embryonic antigen-3 (SSEA-3) expression (   60% of cells) after 7-10 days growing in suspension and did not form @entity44 when injected into immunodeficient @entity19 . @entity69270 -Muse- @entity1386 cells expressed CD105, @entity2393 , @entity12359 , human leukocyte antigen (HLA) class I, @entity1759 , and @entity5399 and low levels of HLA class II, @entity1195 , and @entity2280 . Using lipopolysaccharide-stimulated macrophages and antigen-challenged T-cell assays, we have shown that Muse- @entity1386 cells have anti-inflammatory activities downregulating the secretion of proinflammatory cytokines, such as interferon-y and @entity5 necrosis factor-a. Muse- @entity1386 cells spontaneously gained @entity2533 expression that, in a phosphorylated @entity2536 -dependent manner, might prove pivotal in their observed immunoregulatory activity through decreased expression of T-box transcription factor in T cells. Collectively, the present study has demonstrated the feasibility and efficiency of obtaining Muse- @entity1386 cells that can potentially be harnessed as immunoregulators to treat immune-related disorders. Stem Cells Translational Medicine 2017;6:161-173.

Pluripotent Nontumorigenic Adipose Tissue-Derived Muse Cells have Immunomodulatory Capacity Mediated by XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity2536 :: ('4087', 'Gene') :: ['SMAD2'] @entity1195 :: ('5788', 'Gene') :: ['CD45'] @entity1759 :: ('960', 'Gene') :: ['CD44'] @entity5399 :: ('7070', 'Gene') :: ['CD90'] @entity2533 :: ('7040', 'Gene') :: ['transforming growth factor-b1'] @entity12359 :: ('4907', 'Gene') :: ['CD73'] @entity2393 :: ('3688', 'Gene') :: ['CD29'] @entity2280 :: ('947', 'Gene') :: ['CD34'] @entity69270 :: ('MESH:C052924', 'Chemical') :: ['SSEA-3(+)'] @entity1386 :: ('MESH:D018205', 'Disease') :: ['adipose tissue', 'AT'] @entity44 :: ('MESH:D013724', 'Disease') :: ['teratomas']
@entity2533 :: (7040,Gene) :: ['Transforming Growth Factor-b1']
