Adsorption and plaque formation of @entity4021 virus (FMDV) serotype A12 are inhibited by antibodies to the @entity9615 (A. Berinstein et al., J. Virol. 69:2664-2666, 1995). A @entity1 cell line, K562, which does not normally express alpha(v)beta3 cannot replicate this serotype unless cells are transfected with cDNAs encoding this integrin (K562-alpha(v)beta3 cells). In contrast, we found that a tissue culture-propagated FMDV, type O1BFS, was able to replicate in nontransfected K562 cells, and replication was not inhibited by antibodies to the endogenously expressed integrin alpha5beta1. A recent report indicating that cell surface heparan @entity597 (HS) was required for efficient @entity281 (T. Jackson et al., J. Virol. 70:5282-5287, 1996) led us to examine the role of HS and alpha(v)beta3 in FMDV @entity281 . We transfected normal CHO cells, which express HS but not alpha(v)beta3, and two @entity16337 @entity3569 with cDNAs encoding @entity1 alpha(v)beta3, producing a panel of cells that expressed one or both receptors. In these cells, type A12 replication was dependent on expression of alpha(v)beta3, whereas type O1BFS replicated to high titer in normal CHO cells but could not replicate in @entity16337 cells even when they expressed alpha(v)beta3. We have also analyzed two genetically engineered variants of type O1Campos, vCRM4, which has greatly reduced virulence in @entity291 and can bind to heparin-Sepharose columns, and vCRM8, which is highly virulent in @entity291 and cannot bind to heparin-Sepharose. vCRM4 replicated in wild-type K562 cells and normal, nontransfected @entity435 (HS+ alpha(v)beta3-) cells, whereas vCRM8 replicated only in K562 and CHO cells transfected with alpha(v)beta3 cDNAs. A similar result was also obtained in assays using a vCRM4 virus with an engineered RGD-->KGE mutation. These results indicate that virulent FMDV utilizes the alpha(v)beta3 integrin as a primary receptor for @entity281 and that adaptation of type O1 virus to cell culture results in the ability of the virus to utilize HS as a receptor and a concomitant loss of virulence.

XXXX virus virulent for @entity291 utilizes the @entity9615 as its receptor.

@entity1 :: ('9606', 'Species') :: ['human'] @entity16337 :: ('MESH:D009084', 'Disease') :: ['HS-deficient'] @entity4021 :: ('MESH:D005536', 'Disease') :: ['foot-and-mouth disease'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection', 'infection of type O1'] @entity435 :: ('CHEBI:42485', 'Chemical') :: ['CHO'] @entity291 :: ('9913', 'Species') :: ['cattle'] @entity3569 :: ('10029', 'Species') :: ['CHO cell lines'] @entity9615 :: ('3685', 'Gene') :: ['integrin alpha(v)beta3'] @entity597 :: ('CHEBI:16189', 'Chemical') :: ['sulfate']
@entity4021 :: (MESH:D005536,Disease) :: ['Foot-and-mouth disease']
