OBJECTIVE: To explore the potential markers of @entity14 and the influence of @entity1487 on differentially expressed proteins by using proteomic technology, and to elucidate the mechanism of @entity14 . METHODS: @entity1 @entity14 cell lines of different metastatic potential, Lovo and SW480 were conventionally cultured, and the protein was extracted. 50% inhibitory concentration (IC(50)) of @entity1487 to these two cell lines was measured by @entity98 assay. Proteins of these two cell lines after intervention by @entity1487 at IC(50) were extracted, then 2-dimensional gel electrophoresis was conducted for the proteins. The differential protein spots were examined by mass spectrometry and analyzed by bioinformatics. Difference of expressed proteins in two cell lines before and after the intervention of @entity1487 was validated by Western blot and immunofluorescence. RESULTS: Eleven differentially expressed proteins were identified by 2-dimensional gel electrophoresis and mass spectrometry. The @entity25931 protein and @entity13091 were selected to be examined by Western blot and immunofluorescence. Results revealed that the expression of @entity25931 in Lovo was higher than that in SW480, while the expression of @entity13091 was lower in Lovo. After intervention by @entity1487 at IC(50), the expression of @entity25931 in Lovo decreased more as compared to SW480, while the expression of @entity13091 in SW480 increased more as compared to Lovo. CONCLUSION: There are significant differences in expression of @entity25931 and @entity13091 proteins between Lovo and SW480 cell lines, and the proteins alter regularly after @entity1487 intervention.

[Proteomic research of biomarker of XXXX ].

@entity1 :: ('9606', 'Species') :: ['Human'] @entity25931 :: ('3190', 'Gene') :: ['hnRNP K'] @entity13091 :: ('29943', 'Gene') :: ['PDI'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal carcinoma', 'colorectal cancer metastasis'] @entity1487 :: ('MESH:D005472', 'Chemical') :: ['5-FU']
@entity14 :: (MESH:D015179,Disease) :: ['colorectal cancer metastasis']
