A cDNA clone encoding @entity492 aminotransferase (PVAAT-2) (EC 2.6.1.1) was isolated from the common bean @entity11297 nodule cDNA library. The @entity377 sequence analysis of the full-length cDNA allowed its identification by comparison with sequence databases. The @entity535 sequence of the bean PvAAT-2 showed high similarity with the @entity1605 isoforms described in other leguminous plants. The @entity1472 -terminal region of the PvAAT-2 contains a sequence, which shares common features of plastid transit peptides. Southern blot analysis showed that the PvAAT-2 clone is encoded by a single gene in the @entity19321 genome. Analysis of the PvAAT-2 mRNA levels suggests that the expression of this gene is nodule enhanced. The PvAAT-2 transcript is more abundant in nodules with increased synthesis of @entity7573 and is down-regulated in conditions where ureides accumulate. When plants were supplemented with ureides or with @entity7573 , PvAAT-2 expression was reduced, while it was not affected when plants were treated with @entity208 , an inhibitor of @entity5106 synthesis. On the other hand, the expression of @entity2734 synthetase (another enzyme involved in the synthesis of @entity7573 ) is not affected either by ureides or @entity7573 . These data suggest a role for @entity1605 in the mechanism involved in the synthesis of @entity1568 compounds in bean nodules.

Molecular cloning of the cDNA encoding XXXX aminotransferase from bean root nodules and determination of its role in nodule @entity1568 metabolism.

@entity5106 :: ('CHEBI:74266', 'Chemical') :: ['ureide'] @entity7573 :: ('MESH:D000577', 'Chemical') :: ['amides'] @entity11297 :: ('3885', 'Species') :: ['Phaseolus vulgaris'] @entity1605 :: ('OMIM:217000', 'Disease') :: ['AAT-2'] @entity1472 :: ('CHEBI:46882', 'Chemical') :: ['amino'] @entity1568 :: ('MESH:D009584', 'Chemical') :: ['nitrogen'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity377 :: ('CHEBI:36976', 'Chemical') :: ['nucleotide'] @entity2734 :: ('CHEBI:17196', 'Chemical') :: ['asparagine'] @entity19321 :: ('585', 'Species') :: ['P. vulgaris'] @entity492 :: ('CHEBI:29995', 'Chemical') :: ['aspartate'] @entity208 :: ('MESH:D000493', 'Chemical') :: ['allopurinol']
@entity492 :: (CHEBI:29995,Chemical) :: ['aspartate']
