UNASSIGNED: Monoclonal antibodies (mAbs) specific for leukocyte differentiation molecules (LDMs) were developed during the past few decades to expand reagents for research in ruminants, @entity1707 , and @entity1641 . The specificity of some of the mAb-defined molecules was determined through participation in international workshops. Other molecules identified with mAbs during this time, and more recently with mAbs developed after the workshops, have remained partially characterized. Efforts are now underway to characterize the specificity of these mAbs. As reported here, flow cytometry (FC) was used to screen two sets of hybridomas to determine how many of the hybridomas produce mAbs that detect molecules with up-regulated expression on activated lymphocytes or NK cells. Thirty four hybridomas were identified. Comparison of the patterns of reactivity of the mAbs showed some of the mAbs formed clusters that recognize 5 different molecules. FC showed one cluster recognized @entity39101 . Use of mass spectrometry showed 4 clusters recognized orthologues of @entity25217 , CD50, gp96 and @entity68301 ( @entity68301 ). Verification and documentation that @entity25217 , CD50, and @entity68301 were only up-regulated on activated cells was obtained with PBMC from @entity291 vaccinated with a @entity3069 @entity3068 mutant, Map-relA. @entity25217 and CD50 were up-regulated on NK cells, @entity9214 and CD8 T cells and y   T cells. @entity68301 was only up-regulated on @entity9214 , CD8, and y   T cells. gp96 was detected on granulocytes, monocytes and activated NK cells. Detection was attributable to the binding of gp96 to its receptor CD91.

Characterization and expression of monoclonal antibody-defined molecules on resting and activated XXXX ab, y   T and NK cells.

@entity1707 :: ('9823', 'Species') :: ['pigs'] @entity1641 :: ('9796', 'Species') :: ['horses'] @entity68301 :: ('507627(Tax:9913)', 'Gene') :: ['signaling lymphocytic activation molecule family member 9', 'SLAMF9'] @entity39101 :: ('281861(Tax:9913)', 'Gene') :: ['CD25'] @entity3069 :: ('1764', 'Species') :: ['Mycobacterium avium'] @entity3068 :: ('MESH:D010283', 'Disease') :: ['paratuberculosis'] @entity9214 :: ('407098(Tax:9913)', 'Gene') :: ['CD4'] @entity25217 :: ('281122(Tax:9913)', 'Gene') :: ['CD26'] @entity291 :: ('9913', 'Species') :: ['calves']
@entity291 :: (9913,Species) :: ['bovine']
