Pseudomonas sp. @entity22546 is able to utilize @entity22956 ( @entity22542 ) as its sole @entity1568 source. The role of the xenB gene, encoding xenobiotic reductase B, was investigated using @entity22546 xenB knockout mutants. The xenB mutant degraded @entity22956 to a level that was 10-fold less than that obtained with the wild-type @entity22546 strain. After 60  days of culture with 25 or 50   M @entity22956 , no residual @entity22956 was detected in the supernatants of the wild-type aerobically grown cultures, whereas approximately 90  % of the @entity22956 remained in the xenB mutant cultures. The xenB mutant bacteria exhibited a 10(2)-10(4)-fold decrease in survival rate compared to the wild-type. The expression of DnaK and @entity11217 proteins, two typical @entity1642 proteins (SSPs), in the xenB mutant increased after immediate exposure to @entity22956 , yet dramatically decreased after 4  h of exposure. In addition, DnaK and @entity11217 were more highly expressed in the cultures with 25   M @entity22956 in the medium but showed low expression in the cultures with 50 or 75   M @entity22956 . The expression levels of the dnaK and @entity11217 genes measured by RT-qPCR were also much lower in the xenB genetic background. Analyses of the proteomes of the @entity22546 and xenB mutant cells grown under conditions of @entity22956 stress showed increased induction of several proteins, such as @entity68119 , alginate biosynthesis sensor @entity3793 kinase, and OprH in the xenB mutants when compared to wild-type. However, many proteins, including two SSPs (DnaK and @entity11217 ) and proteins involved in metabolism, exhibited lower expression levels in the xenB mutant than in the wild-type @entity22546 strain. The xenB knockout mutation leads to reduced @entity22956 degradation ability, which renders the mutant more sensitive to @entity22956 stress and results in a lower survival rate and an altered proteomic profile under @entity22956 stress.

Characterization and Proteomic Analysis of the Pseudomonas sp. XXXX ( @entity22542 ) Stress.

@entity22542 :: ('MESH:C009160', 'Chemical') :: ['hexahydro-1,3,5-trinitro-1,3,5-triazine'] @entity1568 :: ('MESH:D009584', 'Chemical') :: ['nitrogen'] @entity3793 :: ('CHEBI:27570', 'Chemical') :: ['histidine'] @entity22546 :: ('5653', 'Gene') :: ['HK-6'] @entity22956 :: ('5962', 'Gene') :: ['RDX'] @entity68119 :: ('79053', 'Gene') :: ['Alg8'] @entity11217 :: ('3329', 'Gene') :: ['groEL', 'GroEL'] @entity1642 :: ('MESH:D015775', 'Disease') :: ['stress shock']
@entity22546 :: (5653,Gene) :: ['HK-6 xenB Knockout Mutant Under RDX']
