@entity162 ( @entity162 ) cells of @entity1 retinae receive synapses from different @entity1246 ( @entity1246 ergic) amacrine cells in the inner plexiform layer (IPL). We addressed the question whether @entity162 cells of the @entity95 and of the @entity35 retina express different types of @entity1246 receptors at these synapses. @entity162 cells were immunolabeled in vertical sections of @entity35 retinae with an antibody against @entity7131 ( @entity7131 ). The sections were double-labeled for the alpha 1, alpha 2, alpha 3, or @entity22473 subunits of the GABAA receptor. Punctate immunofluorescence, which represents synaptic localization, was found for all four subunits. Many of the @entity22473 -immunoreactive puncta coincided with the axon terminals of the @entity7131 -immunolabeled @entity162 cells. Sections and wholemounts of @entity95 retinae were also double labeled for @entity7131 and the rho subunits of the GABAC receptor. @entity95 @entity162 cells were decorated by many rho-immunoreactive puncta, which were shown by electron microscopy to represent synaptic localization. Previous work from our laboratory has shown that the alpha 1, alpha 2, alpha 3, and rho subunits are not found within the same synapse but are expressed at different synaptic sites. Taken together, these results suggest that @entity162 cells of @entity1 retinae express at least three different types of @entity1246 receptors at synaptic sites in the IPL: GABAC receptors, GABAA receptors containing the alpha 1 subunit, and GABAA receptors containing the alpha 3 subunit.

GABAA and GABAC receptors on XXXX rod bipolar cells.

@entity1 :: ('9606', 'Species') :: ['mammalian'] @entity162 :: ('MESH:D001714', 'Disease') :: ['Rod bipolar', 'RB'] @entity1246 :: ('MESH:D005680', 'Chemical') :: ['gamma-aminobutyric acid', 'GABA'] @entity95 :: ('9986', 'Species') :: ['rabbit', 'Rabbit'] @entity7131 :: ('MESH:D020151', 'Disease') :: ['protein kinase C', 'PKC'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity22473 :: ('7453', 'Gene') :: ['gamma 2', 'alpha 1-, alpha 3-, or gamma 2']
@entity1 :: (9606,Species) :: ['mammalian']
