BACKGROUND: Tight-junction (TJ) proteins regulate paracellular permeability. Gut permeability can be modulated by commensal microbiota. Manipulation of the gut microbiota with antibiotics like @entity3637 and @entity5154 turned out to be useful for the treatment of @entity294 induced by @entity2718 or chemotherapy drugs. AIM: To evaluate the effects of the microbiota depletion evoked by the oral administration of @entity5154 and @entity3637 on the intestinal permeability and expression of TJ proteins in @entity19 . METHODS: @entity19 received @entity5154 and @entity3637 orally for 7 days. Intestinal permeability was measured by the @entity68582 ( @entity2823 -dextran) method. The gene expression of TJ proteins in the intestine was determined by real time-PCR. RESULTS: @entity2823 -dextran levels in serum were reduced by half in antibiotic-treated @entity19 , indicating a reduction of intestinal permeability. Antibiotics increased the expression of @entity23453 ( @entity23453 ), @entity30684 ( @entity30684 , and @entity8843 in the ileum and @entity23453 , @entity8841 , and @entity8842 in the colon. CONCLUSION: The combination of @entity5154 and @entity3637 reduce intestinal permeability and increase the gene expression of @entity23453 , @entity30684 ( @entity30684 ), and @entity8843 in the ileum and @entity23453 , @entity8841 , and @entity8842 in the colon.

XXXX and @entity3637 reduce the intestinal permeability in @entity19 and increase the expression of some tight-junction proteins.

@entity23453 :: ('21872(Tax:10090)', 'Gene') :: ['ZO-1', 'zonula occludens 1'] @entity8842 :: ('12740(Tax:10090)', 'Gene') :: ['claudin-4'] @entity8843 :: ('18260(Tax:10090)', 'Gene') :: ['occludin'] @entity3637 :: ('MESH:D001414', 'Chemical') :: ['bacitracin'] @entity19 :: ('10090', 'Species') :: ['mice', 'Mice'] @entity294 :: ('MESH:D003967', 'Disease') :: ['diarrhoea'] @entity8841 :: ('12739(Tax:10090)', 'Gene') :: ['claudin-3'] @entity68582 :: ('MESH:C015219', 'Chemical') :: ['fluorescein-isothiocyanate-dextran'] @entity2823 :: ('MESH:D016650', 'Chemical') :: ['FITC'] @entity5154 :: ('MESH:D009355', 'Chemical') :: ['neomycin'] @entity2718 :: ('1496', 'Species') :: ['Clostridium difficile'] @entity30684 :: ('16456(Tax:10090)', 'Gene') :: ['junctional adhesion molecule A', 'JAM-A']
@entity5154 :: (MESH:D009355,Chemical) :: ['Neomycin']
