1. The kinetics of de-repression of alkaline phosphatase in @entity3881 were studied after the removal of P(i). Enzyme activity appeared about 10min. after removal of P(i), whereas ;enzyme-forming potential' appeared after 6min. 2. Protein synthesis is not impaired for at least 20min. on removal of P(i), but RNA synthesis is considerably diminished. 3. Adding @entity2755 to cells without P(i), just at the time they start to make enzyme-forming potential, does not affect the differential rate of enzyme synthesis compared with total protein. Enzyme-forming potential accumulates to about normal levels in the presence of @entity2755 , even though peptide-bond formation is inhibited by more than 95%. 4. Similar experiments performed with @entity50244 show more complex effects. @entity4316 initially prevents RNA synthesis and also the synthesis of enzyme-forming potential. After some minutes RNA synthesis resumes at a low rate, to be followed 4min. later by enzyme synthesis. Enzyme-forming potential can accumulate in the presence of @entity4316 after the resumption of RNA synthesis. Protein synthesis, initially inhibited by @entity4316 as a consequence of the effect on RNA synthesis, is later directly inhibited by @entity4316 . 5. Adding @entity4316 to de-repressed cells already making enzyme stops enzyme synthesis within 4-5min. Enzyme synthesis resumes, as before, 4min. after the resumption of RNA synthesis. 6. Adding P(i) together with @entity4316 to de-repressed cells synthesizing enzyme does not result in a lower yield of enzyme compared with @entity4316 alone. 7. @entity4316 is less effective an inhibitor of RNA and protein synthesis in P(i)-starved cells if P(i) is also added. 8. These results are discussed in view of the three main models for the regulation of enzyme induction: regulation at the level of transcription only, at translation only, or a coupled model in which transcription requires concomitant translation. It is concluded that the present evidence most powerfully supports the model of transcriptional regulation.

The regulatory process in the de-repression of enzyme synthesis. Alkaline phosphatase of XXXX .

@entity2755 :: ('MESH:D002701', 'Chemical') :: ['chloramphenicol'] @entity3881 :: ('1423', 'Species') :: ['Bacillus subtilis'] @entity4316 :: ('MESH:D003609', 'Chemical') :: ['Actinomycin', 'actinomycin'] @entity50244 :: ('MESH:C030013', 'Chemical') :: ['actinomycin C']
@entity3881 :: (1423,Species) :: ['Bacillus subtilis']
