Radiotherapy continues to cause @entity272 . In this article, with the aid of our @entity1 skin model maintained in ex vivo survival conditions for 15 days, we describe the modifications caused by irradiation and their modulation by a @entity24924 -containing emulsion ( @entity69207 ). Normal @entity1 skin fragments were maintained in organ culture. One ionizing radiation session with 5 Gy was applied. Skin parameters were evaluated 24 h after the radiation session and were compared with a nonirradiated skin fragment: vascular modifications (histology), @entity4 , epithelial proliferation, @entity1192 and @entity650 . Another series of skin fragments was maintained in survival conditions for 15 days after the radiation session to evaluate collagen neosynthesis by fibroblasts and any vascular changes ( @entity2280 ). After irradiation the basal cell proliferation was reduced by approximately 50%. Extensive vasodilation occurred with altered capillary permeability accompanied by decreased @entity2280 transmembrane protein expression. Collagen synthesis and @entity1192 secretion were increased. @entity69207 significantly reduced capillary alterations, restored @entity2280 expression as well as epithelial cell proliferation and significantly decreased collagen synthesis and @entity1192 expression. With this ex vivo @entity1 skin model we confirmed the main modifications induced by radiotherapy as previously described in animal models: decreased basal cell proliferation and endothelial cell alterations and increased collagen synthesis by fibroblasts, probably under the influence of @entity1192 . The effect of @entity69207 emulsion on these histological and biochemical parameters may support its clinical efficacy.

Histochemical and biochemical modifications induced by experimental irradiation of XXXX skin maintained in survival conditions and modulation by application of an emulsion containing @entity24924 .

@entity1 :: ('9606', 'Species') :: ['human'] @entity650 :: ('3569', 'Gene') :: ['IL-6'] @entity24924 :: ('MESH:C009546', 'Chemical') :: ['trolamine'] @entity4 :: ('MESH:D004487', 'Disease') :: ['edema'] @entity69207 :: ('MESH:C423724', 'Chemical') :: ['Biafine'] @entity1192 :: ('3552', 'Gene') :: ['IL-1', 'interleukin (IL)-1alpha'] @entity272 :: ('MESH:D012871', 'Disease') :: ['skin disorders'] @entity2280 :: ('947', 'Gene') :: ['CD34']
@entity1 :: (9606,Species) :: ['human']
