Transcriptional regulation is dependent upon the interactions between the RNA pol II holoenzyme complex and chromatin. RNA pol II is part of a highly conserved multiprotein complex that includes the core mediator and @entity45053 subcomplex. In @entity375 , the @entity45053 subcomplex, composed of @entity68574 , @entity45053 @entity47334 , and @entity68575 , is thought to play important roles in mediating transcriptional control of stress responsive genes. Also central to transcriptional control are histone post-translational modifications. @entity376 methylation, dynamically balanced by @entity376 methyltransferases and demethylases, has been intensively studied, uncovering significant functions in transcriptional control. A key question remains in understanding how these enzymes are targeted during stress response. To determine the relationship between @entity376 methylation, the @entity45053 complex, and transcriptional control, we performed phenotype analyses of @entity375 lacking known @entity376 methytransferases or demethylases in isolation or in tandem with @entity47334 deletions. We show that the RNA pol II @entity45053 submodule components @entity68576 and histone demethylase @entity68577 are required to inhibit pseudohyphal growth - a differentiation pathway induced during nutrient limitation- in rich conditions. @entity375 lacking both @entity47334 and @entity68577 constitutively express @entity5674 , a major regulator for pseudohyphal growth. Interestingly, deleting known @entity5674 activators including @entity5673 , @entity23475 , @entity68578 , @entity39601 , SNF1, @entity5677 , and @entity8422 results in a range of phenotypic suppression. Using chromatin immunoprecipitation, we found that @entity47334 inhibits H3 @entity68579 trimethylation independently of @entity68577 at the @entity5674 locus, suggesting that H3 @entity68579 hypermethylation is locking @entity5674 into a transcriptionally active state. These studies implicate the @entity45053 subcomplex in fine-tuning H3 @entity68579 methylation levels during pseudohyphal differentiation.

Fine-Tuning of Histone H3 XXXX Methylation During Pseudohyphal Differentiation by the CDK Submodule of RNA Polymerase II.

@entity68574 :: ('852053(Tax:4932)', 'Gene') :: ['Ssn2p'] @entity47334 :: ('855706(Tax:4932)', 'Gene') :: ['Ssn8p', 'SSN8'] @entity39601 :: ('852377(Tax:4932)', 'Gene') :: ['TEC1'] @entity68575 :: ('850442(Tax:4932)', 'Gene') :: ['Srb8p'] @entity68579 :: ('851820(Tax:4932)', 'Gene') :: ['Lys4'] @entity8422 :: ('856709(Tax:4932)', 'Gene') :: ['GCN4'] @entity68577 :: ('853583(Tax:4932)', 'Gene') :: ['JHD2'] @entity68578 :: ('851365(Tax:4932)', 'Gene') :: ['MFG1'] @entity376 :: ('CHEBI:25094', 'Chemical') :: ['lysine', 'Lysine'] @entity5673 :: ('856845(Tax:4932)', 'Gene') :: ['FLO8'] @entity5677 :: ('852931(Tax:4932)', 'Gene') :: ['KSS1'] @entity23475 :: ('855200(Tax:4932)', 'Gene') :: ['MSS11'] @entity375 :: ('4932', 'Species') :: ['Saccharomyces cerevisiae', 'Yeast', 'yeast'] @entity68576 :: ('855706;856065', 'Gene') :: ['SSN8/SSN3'] @entity5674 :: ('854836(Tax:4932)', 'Gene') :: ['FLO11'] @entity45053 :: ('856065(Tax:4932)', 'Gene') :: ['CDK8', 'Ssn3p']
@entity68579 :: (851820(Tax:4932),Gene) :: ['Lys4']
