OBJECTIVES: In pulpal revascularization, a protective material is placed coronal to the blood clot to prevent recontamination and to facilitate osteogenic differentiation of mesenchymal stem cells to produce new dental tissues. Although mineral trioxide aggregate (MTA) has been the material of choice for clot protection, it is easily displaced into the clot during condensation. The present study evaluated the effects of recently introduced @entity4799 cements ( @entity4800 and TheraCal LC) on the viability and osteogenic differentiation of @entity1 dental pulp stem cells (hDPSCs) by comparing with MTA Angelus. METHODS: Cell viability was assessed using XTT assay and flow cytometry. The osteogenic potential of hDPSCs exposed to @entity4799 cements was examined using qRT-PCR for osteogenic gene expressions, alkaline phosphatase enzyme activity, @entity622 S staining and transmission electron microscopy of extracellular @entity460 deposits. Parametric statistical methods were employed for analyses of significant difference among groups, with a=0.05. RESULTS: The cytotoxic effects of @entity4800 and TheraCal LC on hDPSCs were time- and concentration-dependent. Osteogenic differentiation of hDPSCs was enhanced after exposure to @entity4800 that was depleted of its cytotoxic components. This effect was less readily observed in hDPSCs exposed to TheraCal LC, although both cements supported extracellular mineralization better than the positive control ( @entity1947 - @entity4744 -based cement). SIGNIFICANCE: A favorable tissue response is anticipated to occur with the use of @entity4800 as a blood clot-protecting material for pulpal revascularization. Further investigations with the use of in vivo animal models are required to validate the potential adverse biological effects of TheraCal LC on hDPSCs.

Cytotoxicity and osteogenic potential of silicate XXXX cements as potential protective materials for pulpal revascularization.

@entity1 :: ('9606', 'Species') :: ['human'] @entity4744 :: ('MESH:D005054', 'Chemical') :: ['eugenol'] @entity1947 :: ('MESH:D015034', 'Chemical') :: ['zinc oxide'] @entity622 :: ('MESH:C010078', 'Chemical') :: ['Alizarin red'] @entity4800 :: ('MESH:C506393', 'Chemical') :: ['Biodentine'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity4799 :: ('MESH:C031293', 'Chemical') :: ['calcium silicate']
@entity460 :: (MESH:D002118,Chemical) :: ['calcium']
