Immunoglobulin G (IgG)-producing plasma cells are the predominant immunoglobulin secreting plasma cells in @entity1 @entity1548 , compared with immunoglobulin A- and immunoglobulin M-producing plasma cells. In this study, the cells expressing mRNA, that encoded the distinct IgG subclasses, were detected using an in situ hybridization technique in 25 periapical lesions. These lesions consisted of 14 periapical @entity738 and 11 @entity9530 . Four oligonucleotide probes were chemically synthesized from IgG subclass-specific hinge region genes to ensure specificity of the probes. Plasma cells expressing mRNA, which coded for the IgG subclasses, were detected in @entity406 -fixed/paraffin wax-embedded sections. Background staining was negligible in all of the sections tested. The in situ hybridization method used in this study was both specific and sensitive for the detection of mRNA encoding each of the four distinct IgG subclasses, whereas the cells retained good morphology. The relative proportions of plasma cells expressing each of the IgG subclass-specific mRNAs in both @entity738 and cysts were as follows: IgG1 (57.4 and 55.5%); IgG2 (34.1 and 34.6%); @entity3172 (4.0 and 4.3%); and IgG4 (4.0 and 5.5%). There were no significant differences between the percentages of plasma cells expressing each of the IgG subclass mRNAs between the two types of lesions. IgG1 producing plasma cells comprised the highest proportion of IgG-producing plasma cells in both types of @entity1548 . IgG2-producing plasma cells were next in abundance, followed by plasma cells for either @entity3172 or IgG4, which were in roughly equivalent numbers.

Relative distribution of plasma cells expressing immunoglobulin G subclass mRNA in @entity1 XXXX using in situ hybridization.

@entity1 :: ('9606', 'Species') :: ['human'] @entity3172 :: ('3502', 'Gene') :: ['IgG3'] @entity738 :: ('MESH:D006099', 'Disease') :: ['granulomas'] @entity406 :: ('MESH:D005557', 'Chemical') :: ['formalin'] @entity1548 :: ('MESH:D010483', 'Disease') :: ['dental periapical lesions', 'periapical lesion'] @entity9530 :: ('MESH:D011842', 'Disease') :: ['radicular cysts']
@entity1548 :: (MESH:D010483,Disease) :: ['dental periapical lesions']
