PURPOSE: Dendritic cells are the most potent antigen presenting cells capable of initiating antitumor immune responses. We previously showed that bacillus Calmette-Guerin (BCG) stimulates cultured @entity1 dendritic cells. We extended these studies and tested the ability of cultured @entity1 dendritic cells to express interleukin @entity687 in response to BCG. We also investigated the T cell stimulatory potential of BCG treated dendritic cells in mixed leukocyte reactions. MATERIALS AND METHODS: Dendritic cells were obtained by culturing plastic adherent mononuclear cells from peripheral blood for 6 days in the presence of @entity2841 and @entity3646 . Spontaneous and BCG stimulated @entity687 protein release into culture supernatants was measured by a quantitative immunoassay. @entity687 gene transcription was assessed by reverse transcription-polymerase chain reaction. Untreated and BCG exposed dendritic cells were compared as stimulators of allogeneic T cell proliferation, measured as @entity665 incorporation. RESULTS: BCG stimulated @entity687 messenger ribonucleic acid expression and @entity687 protein release. @entity687 secretion occurred in a dose and time dependent fashion. BCG induced @entity687 release was further enhanced in the presence of @entity3352 . @entity8153 treated dendritic cells were much more potent T cell stimulators than untreated dendritic cells. CONCLUSIONS: These data demonstrate that BCG enhances the production of @entity687 , a potent chemokine of T cells and granulocytes, as well as the T cell stimulatory potential of @entity1 dendritic cells.

Activation of XXXX dendritic cells by bacillus Calmette-Guerin.

@entity1 :: ('9606', 'Species') :: ['human'] @entity8153 :: ('CHEBI:41001', 'Chemical') :: ['BCG'] @entity3352 :: ('MESH:D007213', 'Chemical') :: ['indomethacin'] @entity665 :: ('CHEBI:53526', 'Chemical') :: ['[3H]thymidine'] @entity3646 :: ('3565', 'Gene') :: ['IL-4'] @entity687 :: ('3576', 'Gene') :: ['IL-8'] @entity2841 :: ('1437', 'Gene') :: ['granulocyte-macrophage colony-stimulating factor']
@entity1 :: (9606,Species) :: ['human']
