BACKGROUND: The analysis of @entity33361 , a promising direct @entity1068 metabolite, in dry blood spots (PEth-DBS) is advantageous due to ease of storage, transportation and minimal invasiveness of capillary blood collection. One potential application of PEth-DBS is to confirm prenatal @entity285 exposure in newborns suspected of @entity13826 ; however, stability of PEth-DBS is largely unknown. METHODS: Phlebotomized samples from 31 adults with a history of @entity988 , admitted to the University of New Mexico Emergency Department, were analyzed for blood @entity285 content and pipetted onto DBS cards (13 spots per @entity1 ). The first spot was analyzed within 2 weeks of collection for a baseline PEth; the remaining 12 spots were allocated into three temperature conditions (room temperature, 4  C, -80  C) for the repeated measures analysis. In addition, 5 newborn DBS samples with a baseline PEth>LOD were obtained from a prospective cohort at UNM and re-analyzed at 4 months after storage at -80  C. A mixed linear model was fitted to examine the effects of temperature, time and temperature-time interaction on PEth degradation over the first 9 months. RESULTS: The baseline PEth levels were 592.8    86.7 ng/ml and 18.3    4.8 ng/ml in adult and newborn samples, respectively. All DBS samples remained positive in successive samples in all temperature conditions. Results of mixed linear model demonstrated a significant effect of temperature (P < 0.001) on PEth degradation over 9 months. CONCLUSIONS: PEth-DBS appears to be relatively stable, especially when stored at lower temperatures. These initial results are encouraging and highlight the PEth-DBS potential in retrospective assessment of @entity285 exposure.

Stability of XXXX in Dry Blood Spot Cards.

@entity1 :: ('9606', 'Species') :: ['patient'] @entity988 :: ('MESH:D000437', 'Disease') :: ['alcoholism'] @entity33361 :: ('MESH:C051521', 'Chemical') :: ['phosphatidylethanol'] @entity13826 :: ('MESH:D063647', 'Disease') :: ['FASD'] @entity285 :: ('D000431', 'Chemical') :: ['alcohol'] @entity1068 :: ('MESH:D000431', 'Chemical') :: ['ethanol']
@entity33361 :: (MESH:C051521,Chemical) :: ['Phosphatidylethanol']
