BACKGROUND: The neuropeptide neuromodulator and hormone @entity398 (OT) activates signaling pathways involved in mRNA translation in response to endoplasmic reticulum stress and reduces @entity32 associated with experimental @entity1960 in rats. The anti-inflammatory effects of OT may serve a vital role in the development, survival and function of newborn-type enterocytes during microbial gut colonization, which coincides with the milk suckling period when OT receptor expression peaks in the gut. Furthermore, @entity19 deficient in the OT receptor have abnormal gut structure and function, underscoring OT's developmental importance. METHODS: We tested the effect of OT upon lipopolysaccharide (LPS)-induced markers of the inflammatory response in Caco2BB gut cells in vitro using automated immunocapillary electrophoresis. RESULTS: We demonstrate that OT suppresses NF-kB signaling and presumably inflammatory transcriptional programs, which are unleashed by LPS through the modulation of IkB. We show that OT counteracts LPS-elicited silencing of the unfolded protein response, a pathway limiting endoplasmic reticulum stress by suppressing protein translation. OT selectively activates dsRNA-activated kinase ( @entity9657 ), @entity5594 ( @entity5594 ), immunoglobulin binding protein ( @entity1477 ), @entity4639 (TNFa-induced protein 3) and @entity8580 requiring enzyme 1a ( @entity5593 ). OT inactivates @entity12465 ( @entity15204 ) without significant activation of @entity7952 ( @entity7952 ). CONCLUSIONS: Mild, preemptive stimulation of endoplasmic reticulum stress sensors by OT may precondition newborn enterocytes to resist apoptosis associated with @entity32 and may support their differentiation and development by modulating cellular metabolism. GENERAL SIGNIFICANCE: OT may protect enterocytes and other cell types, such as neurons, from stress-related complications during postnatal development.

XXXX opposes effects of bacterial endotoxin on ER-stress signaling in Caco2BB gut cells.

@entity398 :: ('MESH:D010121', 'Chemical') :: ['oxytocin'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity9657 :: ('5610', 'Gene') :: ['PKR'] @entity15204 :: ('1965', 'Gene') :: ['eIF2a'] @entity1477 :: ('3309', 'Gene') :: ['BiP'] @entity8580 :: ('MESH:D007294', 'Chemical') :: ['inositol'] @entity12465 :: ('83939', 'Gene') :: ['eukaryotic translation initiation factor 2a'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity4639 :: ('7128', 'Gene') :: ['A20'] @entity5594 :: ('7494', 'Gene') :: ['XBP1', 'X-box binding protein 1'] @entity7952 :: ('9451', 'Gene') :: ['protein kinase RNA-like endoplasmic reticulum kinase', 'PERK'] @entity1960 :: ('MESH:D003092', 'Disease') :: ['colitis'] @entity5593 :: ('2081', 'Gene') :: ['IRE1a']
@entity398 :: (MESH:D010121,Chemical) :: ['Oxytocin']
