Efficient gene transfer is a prerequisite for analysing regulation of transfected promoters. We combined the DNA binding property of the cationic polymer @entity12279 ( @entity8681 ) and the potent endocytic activity of adenovirus in a @entity8681 -DNA-adenovirus complex which provided efficient plasmid delivery in differentiated cultured cells. We transfected 3T3-F442A adipocytes, C2.7 myocytes and FAO @entity157 cells with a construct containing the @entity2235 promoter fused to the @entity2755 acetyltransferase (CAT) gene, using a combination of @entity8681 and 200 p.f.u. per cell of replication-deficient type 5 adenovirus. Resulting CAT activities varied according to the cell type reaching about 0.6, 8 and 38 units/mg protein for respectively 3T3-F442A, FAO and C2.7 cells. Increases in transfection efficiencies were 140- to 300-fold when compared with those obtained with @entity8681 alone. Then we tested physiologically regulated promoters: the @entity7954 carboxykinase gene promoter in 3T3-F442A or FAO cells and the hexokinase II gene promoter in C2.7 myocytes. Gene expression was appropriately increased by @entity12880 , @entity619 and insulin for 3T3-F442A, FAO and C2.7 cells, respectively. Thus, the combination of @entity8681 and adenovirus is a simple, efficient, inexpensive and versatile method of gene transfer which is applicable to several differentiated cells and provides a physiologically coherent transgene regulation. We name this method @entity8681 -adenofection.

Adenovirus enhancement of XXXX -mediated transfer of regulated genes in differentiated cells.

@entity12880 :: ('MESH:D002994', 'Chemical') :: ['clofibrate'] @entity2235 :: ('10633', 'Species') :: ['simian virus 40'] @entity12279 :: ('MESH:D011094', 'Chemical') :: ['polyethylenimine'] @entity7954 :: ('MESH:D010728', 'Chemical') :: ['phosphoenolpyruvate'] @entity619 :: ('MESH:D003907', 'Chemical') :: ['dexamethasone'] @entity8681 :: ('CHEBI:53231', 'Chemical') :: ['PEI'] @entity157 :: ('MESH:D006528', 'Disease') :: ['hepatoma'] @entity2755 :: ('MESH:D002701', 'Chemical') :: ['chloramphenicol']
@entity12279 :: (MESH:D011094,Chemical) :: ['polyethylenimine']
