Oxidized low-density lipoprotein ( @entity29439 ) is an atherogenic lipoprotein. It has been suggested that @entity29439 causes @entity409 by decreasing the release of endothelium-derived factors (EDRF-NO) or increasing the inactivation of EDRF-NO. The mechanism by which @entity29439 causes dysfunctional NO during early stages of @entity731 is not clear. The purpose of this study was to examine the role of @entity29439 on @entity842 synthetase (eNOS), protein kinase C (PKC) activities and @entity268 production in @entity291 aortic endothelial cells (BAEC). @entity29439 stimulated PKC activity of BAEC but it inhibited both eNOS activity and @entity268 production. @entity29439 partially inhibited the forskolin stimulated @entity268 production. Furthermore, we observed that @entity7410 treatment decreased the activity of eNOS in a concentration dependent manner. @entity750 which has a profound inhibitory effect on @entity268 production also stimulated eNOS activity. Pertusis toxin treatment blocked the stimulatory action of @entity750 on the stimulation of eNOS activity. Our results thus suggest that @entity29439 inhibit the endothelium-dependent relaxation. One possible mechanism is that @entity29439 stimulates PKC activity, which in turn increases the phosphorylation of the Gi-protein. Inhibition of Gi-protein then leads to reduced release of NO from endothelial cells and thus causes @entity409 .

Effects of oxidized low density lipoprotein on XXXX synthetase and protein kinase C activities in @entity291 endothelial cells.

@entity268 :: ('CHEBI:17489', 'Chemical') :: ['cAMP'] @entity409 :: ('MESH:D008107', 'Disease') :: ['endothelial dysfunction'] @entity842 :: ('MESH:D009569', 'Chemical') :: ['nitric oxide'] @entity750 :: ('MESH:D012701', 'Chemical') :: ['serotonin', 'Serotonin'] @entity731 :: ('MESH:D050197', 'Disease') :: ['atherosclerosis'] @entity29439 :: ('CHEBI:60151', 'Chemical') :: ['Ox-LDL'] @entity291 :: ('9913', 'Species') :: ['bovine'] @entity7410 :: ('CHEBI:64211', 'Chemical') :: ['8Br-cAMP']
@entity842 :: (MESH:D009569,Chemical) :: ['nitric oxide']
