UNASSIGNED: Intervertebral disc (IVD) @entity528 is responsible for various spine pathologies and present clinical treatments are insufficient. Concurrently, the mechanisms behind @entity1797 are still not completely understood, so as to allow development of efficient tissue engineering approaches. A model of @entity35 @entity1797 directly coupled to herniation is here proposed in a pilot study. @entity130 is induced by needle puncture, using two different needles gauges: a low caliber 25-G needle and a high caliber 21-G needle. Histological, biochemical and radiographic @entity528 was evaluated at 2 and 6 weeks post-injury. We show that the larger caliber needle results in a more extended histological and @entity1797 , compared to the smaller one. TUNEL quantification indicates also increased cell death in the 21-G group. Analyses of collagen type I (Picrosirius red staining), collagen type II (immunofluorescence) and GAG content (Blyscan assay) indicate that @entity528 features spontaneously recover from 2 to 6 weeks, for both needle types. Moreover, we show the occurrence of @entity547 proportional to the needle gauge. The number of @entity22519 + macrophages present, as well as cell apoptosis within the herniated tissue are both proportional to @entity547 volume. Moreover, @entity547 formed after lesion tend to spontaneously diminish in volume after 6 weeks. Finally, @entity16309 is increased in the @entity547 in the 21-G group at 2 weeks. This model, by uniquely combining @entity1797 in the same animal, may help to understand mechanisms behind IVD pathophysiology, such as @entity547 formation and spontaneous regression. This article is protected by copyright. All rights reserved.

Joint analysis of IVD herniation and @entity528 by XXXX caudal needle puncture model.

@entity1797 :: ('MESH:D055959', 'Disease') :: ['radiographic degeneration within the IVD', 'IVD degeneration and IVD herniation', 'IVD degeneration'] @entity16309 :: ('171045(Tax:10116)', 'Gene') :: ['MMP3'] @entity22519 :: ('287435(Tax:10116)', 'Gene') :: ['CD68'] @entity547 :: ('MESH:D006547', 'Disease') :: ['hernia', 'hernias'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity528 :: ('MESH:D009410', 'Disease') :: ['degeneration'] @entity130 :: ('MESH:D014947', 'Disease') :: ['Disc injury']
@entity35 :: (10116,Species) :: ['rat']
