Cycling probe technology ( @entity1191 ) represents a simple method for detection of DNA target sequences. Cycling probe technology utilizes a chimeric DNA-RNA-DNA probe which is cleaved by RNase H when hybridized with its complementary target. Probe cleavage in the presence or absence of target generates @entity1191 product or background, respectively. Addition of non-homologous DNA into the @entity1191 reaction affects the background and @entity1191 product. Low amounts of @entity1 DNA (4-40 ng) result in high background while higher amounts (40-400 ng) inhibit the reaction. The simultaneous addition of @entity8037 and @entity4527 into the @entity1191 reaction containing @entity1 DNA resulted in a significant release of the inhibition and a reduction of background. The presence of @entity8037 alone caused an increase of probe cleavage whereas addition of EGTA increased the specificity of the @entity1191 . A possible mechanism by which @entity8037 could lead to this improvement of @entity1191 has been proposed. Using a membrane-binding assay, the authors demonstrated that @entity1 DNA competes with the probe for binding to RNase H. Furthermore, by using a DNA-agarose column, it has been shown that such RNase H-DNA binding can be disrupted by @entity8037 . Within the @entity1191 reaction, similar @entity8037 -mediated displacement of RNase H from @entity1 DNA could lead to an improved @entity1191 efficiency.

XXXX -mediated improvement of cycling probe reaction.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1191 :: ('CHEBI:27656', 'Chemical') :: ['CPT'] @entity4527 :: ('MESH:D004533', 'Chemical') :: ['EGTA'] @entity8037 :: ('MESH:D013096', 'Chemical') :: ['spermine']
@entity8037 :: (MESH:D013096,Chemical) :: ['Spermine']
