BACKGROUND AND PURPOSE: @entity879 ( @entity879 ) is a severe and potentially lethal clinical syndrome. @entity14507 ( @entity12315 ) is a natural plant-derived compound with anti- @entity5 activities. Recently, @entity12315 has also been shown to have anti-inflammatory properties. Here, we tested the hypothesis that @entity12315 would suppress endotoxin-induced @entity879 . EXPERIMENTAL APPROACH: We investigated the therapeutic potential of @entity12315 in a @entity19 model of @entity8688 /Lipopolysaccharide ( @entity17234 / @entity558 )-induced @entity879 . The efficacy of @entity12315 treatment was assessed by survival, liver histopathology, serum levels of @entity3929 transaminase, pro-inflammatory cytokines and number of activated liver macrophages. Effects of @entity12315 on the expression of two miRNAs, 106a and 20b, and their predicted target gene were measured by qRT-PCR and Western blotting. Effects of @entity12315 on the release of @entity637 from RAW264.7 macrophages transfected with mimics of these miRNAs and activated by @entity558 was assessed by elisa. KEY RESULTS: @entity12315 treatment protected @entity19 from @entity879 symptoms and reduced the number of activated liver macrophages. @entity12315 increased expression of @entity68705 and @entity68706 in liver mononuclear cells and decreased expression of their predicted target gene IL-1 receptor-associated kinase 4 ( @entity32800 ), involved in signalling from @entity1990 ( @entity1990 ). In vitro transfection of RAW264.7 cells using miRNA mimics of @entity68705 and 20b decreased expression of @entity32800 and of @entity637 secretion, following @entity558 stimulation. CONCLUSIONS AND IMPLICATIONS: @entity12315 attenuated @entity17234 / @entity558 -induced @entity879 by regulating the expression of unique miRNAs that target key molecules in the @entity1990 inflammatory pathway. @entity12315 may represent a potential novel hepatoprotective agent.

@entity14507 attenuates @entity558 -mediated XXXX by regulating miRNAs to target @entity32800 and suppress Toll-like receptor signalling.

@entity14507 :: ('MESH:C016392', 'Chemical') :: ["3,3'-Diindolylmethane"] @entity558 :: ('MESH:C536528', 'Disease') :: ['LPS'] @entity68706 :: ('723923(Tax:10090)', 'Gene') :: ['miR-20b'] @entity32800 :: ('266632(Tax:10090)', 'Gene') :: ['IRAK4'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse'] @entity879 :: ('MESH:D017114', 'Disease') :: ['ALF', 'Acute liver failure'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity12315 :: ('CHEBI:50182', 'Chemical') :: ['DIM'] @entity17234 :: ('14419(Tax:10090)', 'Gene') :: ['GalN'] @entity3929 :: ('D000409', 'Chemical') :: ['alanine'] @entity8688 :: ('CHEBI:60313', 'Chemical') :: ['D-galactosamine'] @entity68705 :: ('723829(Tax:10090)', 'Gene') :: ['miR-106a'] @entity1990 :: ('21898(Tax:10090)', 'Gene') :: ['TLR4', 'Toll-like receptor 4'] @entity637 :: ('21926(Tax:10090)', 'Gene') :: ['TNF-a']
@entity879 :: (MESH:D017114,Disease) :: ['acute liver failure']
