OBJECTIVES: Antibiotic susceptibilities, genes mediating @entity3405 and @entity7646 resistance, and serotypes were analysed for strains of @entity328 . METHODS: A total of 392 strains of @entity2260 were isolated from paediatric @entity1 with community-acquired @entity55 between May 2002 and 2004. All strains were classified into six genotype patterns according to the mutations found in the pbp1a, pbp2x and pbp2b genes identified by PCR. These results are represented by adding 'g', indicating genotypic identification. RESULTS: Thirty-nine per cent of the isolates showed mutations in either one or two PBP genes (gPISP, where PISP stands for @entity114 -intermediate resistant @entity2260 ) and 52.3% had mutations in three genes (gPRSP, where PRSP stands for @entity114 -resistant @entity2260 ). The majority of the strains had a macrolide resistance gene: mefA, (30.6%); ermB, (48.5%); or both mefA and ermB, (7.7%). The most frequent serotypes of these strains were: 6B (23.2%), 23F (17.6%), 19F (17.3%), 14 (10.5%) and 6A (8.2%). Serotypes of the seven-valent conjugate vaccine covered 70.9% of all isolates, and 89.8% of gPRSP. Serotypes of the strains with cefotaxime MICs of > or =2 mg/L were almost all of a vaccine type. CONCLUSIONS: The results suggest that introduction of conjugate vaccines into @entity1 and @entity1 is necessary for the prevention of @entity7706 in Japan.

Antibiotic susceptibility according to genotype of @entity114 -binding protein and macrolide resistance genes, and serotype of @entity328 isolates from community-acquired XXXX in @entity1 .

@entity1 :: ('9606', 'Species') :: ['children', 'patients', 'infants'] @entity7706 :: ('MESH:D011008', 'Disease') :: ['pneumococcal infections'] @entity7646 :: ('CHEBI:25106', 'Chemical') :: ['macrolide'] @entity3405 :: ('CHEBI:35627', 'Chemical') :: ['beta-lactam'] @entity55 :: ('MESH:D011014', 'Disease') :: ['pneumonia'] @entity2260 :: ('2104', 'Species') :: ['S. pneumoniae'] @entity114 :: ('MESH:D010406', 'Chemical') :: ['penicillin'] @entity328 :: ('1313', 'Species') :: ['Streptococcus pneumoniae']
@entity55 :: (MESH:D011014,Disease) :: ['pneumonia']
