PURPOSE: Angiogenesis refers to the latter stage of vascular development. It has been reported that angiopoietin-like factor @entity267 -derived transcript 6 ( @entity69396 ) encodes a protein homologous to angiopoietins that could play a critical role in blocking a receptor of angiopoietin ( @entity878 ) and therefore contribute to the avascularity and transparency of the @entity267 in the developing embryo and the adult. This study was focused on isolation and characterization of the @entity69396 promoter. METHODS: Rapid amplification of cDNA ends (5'-RACE) was used to isolate the @entity69396 promoter from an adaptor-ligated genomic DNA fragment library and to identify the transcription initiation site of the @entity69396 gene. The RNase protection assay was performed to confirm the initiation site. The sequence similarity, binding sites for putative transcription factors, and transcriptional activity of @entity1 and @entity19 @entity69396 promoters were compared. Corneal and noncorneal cells from @entity1 and other animals were transiently transfected with @entity69396 promoter- @entity2755 acetyltransferase (CAT) reporter constructs to analyze the transcriptional activity of the promoter. RESULTS: A 2956-bp @entity1 @entity69396 promoter fragment and a 3142-bp @entity19 @entity69396 promoter fragment were isolated. The major transcription initiation sites of the @entity1 and @entity19 @entity69396 genes were located at 224 and 168 bp, respectively, upstream of the translation initiation site. @entity1 and @entity19 @entity69396 promoter sequences were very similar. Both promoters were minus TATA and CAAT boxes close to the transcription initiation site. Transfection into @entity1 corneal and noncorneal cells and into nonhuman cells revealed that the @entity1 @entity69396 promoter probably contains positive and negative cis-regulatory elements that modulate cell, tissue, and species specificity. The @entity1 @entity69396 promoter contains four interferon (IFN)-stimulated response elements (ISREs). No ISREs could be identified in the @entity19 promoter. @entity3027 stimulated transcriptional activity of the @entity1 promoter. CONCLUSIONS: The @entity1 and @entity19 @entity69396 promoters have similar sequences and share many cis-regulatory elements. @entity3027 appears to have an important role in regulating transcription of the @entity1 , but not the @entity19 , @entity69396 promoter.

Characterization of XXXX and @entity19 angiopoietin-like factor CDT6 promoters.

@entity1 :: ('9606', 'Species') :: ['humans', 'human', 'Human'] @entity878 :: ('7010', 'Gene') :: ['Tie2'] @entity267 :: ('MESH:D065306', 'Disease') :: ['cornea'] @entity69396 :: ('10218', 'Gene') :: ['CDT6'] @entity19 :: ('10090', 'Species') :: ['mouse'] @entity2755 :: ('MESH:D002701', 'Chemical') :: ['chloramphenicol'] @entity3027 :: ('3439', 'Gene') :: ['IFN-alpha']
@entity1 :: (9606,Species) :: ['human']
