A novel gene lsp40 from @entity69783 (LsNPV) which was homologous to the @entity10669 gene from @entity20422 ) was localized in the EcoRV-5.5 kb fragment of LsNPV genome DNA and was sequenced. The open reading frame (ORF) of lsp40 was 906 bp long and encoded an approximately 40 kDa peptide consisting of 302 @entity535 residues. The Isp40 shares 80.4% and 70.4% identity of @entity377 and @entity535 sequence, respectively, to the AcNPV- @entity10669 gene. The identity in promoters of both genes was as high as 100%. It was found that there were two TATA and GC boxes, three ACGT motifs for initiation of early gene transcription and one typical TTAAG core sequence for initiation of late gene transcription at both gene's 5' end, and two AATAAA tail signals at 3' end. The Isp40 started to express 2 hours after being transfected into the Ls cells to produce an approximately 40 kDa protein. The expression of the lsp40 gene might also inhibit the apoptosis of the Ls cells induced by removing serum. The expression of lsp40 in Vero cells delayed the apoptosis of the Vero cells induced by @entity9925 infection. The lsp40 gene rescued vAcAnh (a kind of AcNPV @entity10669 -deletion mutant) in the Sf9 cells resulting in inhibition of the apoptosis and production of polyhedra. All these data suggested that the lsp40 gene was a homologous and functional gene similar to AcNPV @entity10669 gene.

Identification of apoptosis-inhibiting gene in XXXX .

@entity20422 :: ('46015', 'Species') :: ['Autographa californica Nuclear Polyhedrosis Virus (AcNPV'] @entity535 :: ('CHEBI:33704', 'Chemical') :: ['amino acid'] @entity9925 :: ('138950', 'Species') :: ['poliovirus'] @entity377 :: ('CHEBI:36976', 'Chemical') :: ['nucleotide'] @entity10669 :: ('3592', 'Gene') :: ['p35'] @entity69783 :: ('41714', 'Species') :: ['leucania separata nuclear polyhedrosis virus']
@entity69783 :: (41714,Species) :: ['Leucania separata nuclear polyhedrosis virus']
