UNASSIGNED: @entity1487 ( @entity1487 ) is one among the anti- @entity5 agents in FOLFORINOX treatment along with @entity901 and @entity2600 for the treatment of @entity14 . Despite its potential activity on the @entity5 cells, it lacks site specificity partly attributed by its biodistribution to healthy cells resulting in toxic effects to healthy cells. Therefore, we have formulated @entity1487 enteric-coated nanoparticles (5-FUEC) to localize the drug in the colon area that enables its prolonged presence in target area in a sustained manner. The current work emphasizes on enhanced anti- @entity5 activity of 5-FUEC sequencing its apoptotic activity on HCT 116 @entity14 cell lines in vitro. @entity98 assay exhibited 5.5-fold decrease in IC50 value of nanoparticles comparable to @entity1487 . @entity1949 with irregular edges in nucleus of cells justified its improved activity. Furthermore, flow cytometric analysis confirms the majority of cells gated in early apoptotic (39.75%) and late apoptotic phase (36.25%). Acridine orange/ @entity5611 staining (AO/EB) exhibited cells with red fluorescence (indicating apoptosis) comparable to the control and @entity1487 . y-Scintigraphic studies determined the applicability and feasibility of the enteric coating with mean gastric emptying time, mean intestinal transit time and mean colon arrival time of 1.89        0.03, 2.15        0.05 and 4.03        0.27   h, respectively. Moreover, nanoparticulate approach was found significant in reducing @entity5 size and volume in xenograft @entity5 models in vivo along with sustained release. These superior anti- @entity5 activities exhibited by 5-FUEC indicated that it could be a potential alternative to chemotherapy for @entity14 .

@entity1487 enteric-coated nanoparticles for improved apoptotic activity and therapeutic index in treating XXXX .

@entity901 :: ('MESH:C030110', 'Chemical') :: ['oxaliplatin'] @entity1949 :: ('MESH:D012892', 'Disease') :: ['Nuclear fragmentation'] @entity2600 :: ('MESH:C051890', 'Chemical') :: ['irinotecan'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity5611 :: ('MESH:D004996', 'Chemical') :: ['ethidium bromide'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity14 :: ('MESH:D015179', 'Disease') :: ['colorectal cancer'] @entity1487 :: ('MESH:D005472', 'Chemical') :: ['5-Fluorouracil', '5-FU', '5-fluorouracil']
@entity14 :: (MESH:D015179,Disease) :: ['colorectal cancer']
