Currently, there is no effective single antigen and there are only a very limited number of complex antigens for the diagnosis of @entity0 (BC). In this study, we used serological analysis of recombinant cDNA expression libraries (SEREX) in combination with phage display technology to screen complex autoantigens from the sera of BC @entity1 . The cDNA expression library was constructed using tissue samples of three @entity1 with BC at as early as stage T1N0M0. The serum samples of ten @entity1 , including the three @entity1 who provided tissue samples, as well as five healthy @entity1 subjects as controls were used to screen the library. All seven autoantigens were identified from the library by four rounds of screening and matched the existing genes after a blast search using NCBI-BLAST. Then, the expression conditions of the autoantibodies of the seven autoantigens and anti- @entity2355 in the sera from 100 BC @entity1 and 50 healthy donors were examined by gray values. The data were analyzed by the area under the receiver operating characteristic (ROC) curve and logistic regression diagnostic models. In the end, a panel of complex autoantigens consisting of B11 ( @entity14813 ), @entity17375 ( @entity29473 ), B119 ( @entity2355 ), B130 ( @entity56307 ), and @entity2355 , which had a sensitivity of 87  % and a specificity of 76  %, were identified. The area under the curve (AUC) of the complex antigens was 0.872, which is significantly greater than that of anti- @entity2355 alone (AUC   =   0.634) for the diagnosis of BC. Thus, this panel of complex antigens provides a promising strategy for the diagnosis of early-stage BC.

Identification of a panel of complex autoantigens ( @entity14813 , @entity29473 , XXXX , and @entity56307 ) in combination with @entity2355 for the diagnosis of @entity0 .

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity29473 :: ('11331', 'Gene') :: ['PHB2'] @entity0 :: ('MESH:D001943', 'Disease') :: ['early-stage breast cancer'] @entity56307 :: ('2712', 'Gene') :: ['GK2'] @entity17375 :: ('4713', 'Gene') :: ['B18'] @entity2355 :: ('4582', 'Gene') :: ['MUC1', 'CA15-3'] @entity14813 :: ('3958', 'Gene') :: ['LGALS3']
@entity2355 :: (4582,Gene) :: ['MUC1']
