PURPOSE: We have shown previously that Bruch's membrane (BM) aging decreases @entity989 ( @entity989 ) phagocytosis. Herein, we determine the effects of BM reengineering on @entity989 phagocytosis. METHODS: BM explants were dissected from young and old donor eyes. Some old BM explants were reengineered by cleaning with @entity6000 and/or coating with extracellular matrix (ECM) ligands. @entity1 cell-derived ECM (ARPE-ECM) modified ("aged") by @entity11495 was subjected to similar treatments. @entity1 cells were then cultured to confluence onto the different surfaces. Fluorescently-labeled @entity291 rod outer segments (ROS) were fed to cells with or without aVb5 integrin antibody. Image acquisition and phagocytosis quantification was performed by fluorescence microscopy and ImageJ analysis. RESULTS: Cleaning old donor-derived BM with detergent does not increase the uptake of ROS, but a combination of cleaning and coating with ECM ligands significantly increases @entity989 phagocytosis (54.9    6.2 vs. 83.5    6.5 arbitrary units; P < 0.05) to levels closer to young donor BM (123.6    9.9 arbitrary units). Similar effects were observed on @entity3542 -modified ARPE-ECM subjected to the same treatments. Incubation of aVb5 blocking antibody with ROS significantly decreased @entity989 phagocytosis. CONCLUSIONS: The detrimental effects of aging BM on @entity989 phagocytosis can be reversed by reengineering the BM surface with detergent cleaning and recoating with ECM ligands. TRANSLATION RELEVANCE: These results demonstrate that the therapeutic success of transplanted @entity989 cells may require, at least in part, reengineering of diseased BM to make it a more suitable environment for attachment, survival and proper functioning of the @entity989 .

Reengineering XXXX Bruch's Membrane Increases Rod Outer Segment Phagocytosis by @entity1 Retinal Pigment Epithelium.

@entity1 :: ('9606', 'Species') :: ['ARPE-19'] @entity6000 :: ('MESH:D017830', 'Chemical') :: ['Triton X-100'] @entity3542 :: ('CHEBI:16301', 'Chemical') :: ['nitrite'] @entity11495 :: ('MESH:D012977', 'Chemical') :: ['sodium nitrite'] @entity989 :: ('MESH:D012164', 'Disease') :: ['retinal pigment epithelium', 'RPE'] @entity291 :: ('9913', 'Species') :: ['bovine']
@entity1 :: (9606,Species) :: ['Human']
