PURPOSE: To investigate the association of the @entity33824 alleles with the occurrence of Recurrent @entity2208 . METHODS: A case-control study of 200 @entity1 aged 18 to 35 years, consisting of a convenience sample of 100 @entity1 who had idiopathic recurrent @entity2208 and 100 @entity1 without @entity5852 and with two or more @entity1 . Peripheral blood genomic DNA was extracted from 500l of Buffy Coat stored at -20  C. HLA typing was performed by the PCR-SSOP method (Polymerase Chain Reaction - Specific Sequence of Oligonucleotides Probes, One Lambda(r), CA, USA). The regions of the amplified DNA were exon 2 and 3 for the A and B loci and only exon 3 for the @entity1533 locus. The HLA FUSIONTM program (One Lambda, Canoga Park, CA, USA, version 3.0) was used for @entity1529 , @entity1530 and @entity1533 genotyping. Absolute frequencies and percentages and calculation of mean and standard deviation were used for standard statistical analysis. The qualitative variables were compared by the   2 test with Yates correction or by Fisher's exact test. The odds ratio with the 95%CI was used for the comparisons, with the level of significance set at p<0.05. RESULTS: The frequency of the A*34 allele was significantly higher in the case group compared to control (4.0 versus0.5%; p<0.05). Alleles A*24 (6.0 versus12.5%; p<0.05) and B*35 (8.0 versus20.5%; p<0.05) were significantly less frequent in the case group. Among the class II alleles, @entity1533 *03 showed a slightly higher frequency in the case group (11.0 versus5.5%, p = 0.056). CONCLUSIONS: It was shown that the @entity1529 *34 allele is a risk factor for recurrent @entity2208 , while the @entity1529 *24 and @entity1530 *35 alleles are associated with protection, and no allele of the @entity1533 locus was associated with RSA.

[HLA alleles association and recurrent XXXX in a S  o Luis/Maranh  o population, in Brazilian Northeastern region].

@entity1 :: ('9606', 'Species') :: ['women', 'children'] @entity2208 :: ('MESH:D000022', 'Disease') :: ['Spontaneous Abortion', 'spontaneous abortion'] @entity5852 :: ('MESH:D000031', 'Disease') :: ['abortion'] @entity33824 :: ('3105;3106;3123', 'Gene') :: ['HLA-A, -B and -DRB1'] @entity1529 :: ('3105', 'Gene') :: ['HLA-A'] @entity1530 :: ('3106', 'Gene') :: ['HLA-B'] @entity1533 :: ('3123', 'Gene') :: ['HLA-DRB1', 'DRB1']
@entity2208 :: (MESH:D000022,Disease) :: ['spontaneous abortion']
