The @entity3668 regulates the stability of microtubules within neurons in the central nervous system. In turn, microtubules are responsible for the remodeling of the cytoskeleton that ultimately leads to the formation or pruning of new connections among neurons. As a consequence, dysfunction of tau is associated with many forms of @entity303 as well as @entity195 . In the brain, tau activity is regulated by its phosphorylation state. Phosphorylation is a post-translational modification of proteins that adds a @entity4613 group to the side chain of an @entity3689 . Phosphorylation at key locations in the tau sequence leads to a higher or lower affinity for microtubules. In @entity195 , tau is present in an abnormal phosphorylation state. However, studying the effect of phosphorylation experimentally has been extremely challenging as there is no viable way of exactly selecting the location and the number of phosphorylated sites. For this reason, researchers have turned to pseudophosphorylation. In this technique, actual phosphorylation is mimicked by mutating the selected @entity3689 into @entity3264 or @entity5906 . Whether this methodology is equivalent to actual phosphorylation is still open to debate. In this study, we will show that phosphorylation and pseudophosphorylation are not exactly equivalent. Although for larger aggregates the two techniques lead to similar structures, the kinetics of the process may be altered. In addition, very little is known about the impact that this may have on the early stages of aggregation, such as nucleation and conformational rearrangement. In this study, we show that the two methods may produce a similar ensemble of conformations, even though the kinetic and chemical details that lead to it are quite different.

Impact of Phosphorylation and Pseudophosphorylation on the Early Stages of Aggregation of the XXXX .

@entity3264 :: ('D018698', 'Chemical') :: ['glutamate'] @entity303 :: ('MESH:D003704', 'Disease') :: ['dementia'] @entity4613 :: ('D010710', 'Chemical') :: ['phosphate'] @entity5906 :: ('D001224', 'Chemical') :: ['aspartate'] @entity195 :: ('MESH:D000544', 'Disease') :: ["Alzheimer's disease"] @entity3689 :: ('D000596', 'Chemical') :: ['amino acid'] @entity3668 :: ('4137', 'Gene') :: ['microtubule-associated protein tau']
@entity3668 :: (4137,Gene) :: ['Microtubule-Associated Protein Tau']
