Radiotherapy continues to cause @entity272 . In this article, with the aid of our @entity1 skin model maintained in ex vivo survival conditions for 15 days, we describe the modifications caused by irradiation and their modulation by a @entity24924 -containing emulsion ( @entity69207 ). Normal @entity1 skin fragments were maintained in organ culture. One ionizing radiation session with 5 Gy was applied. Skin parameters were evaluated 24 h after the radiation session and were compared with a nonirradiated skin fragment: vascular modifications (histology), @entity4 , epithelial proliferation, @entity1192 and @entity650 . Another series of skin fragments was maintained in survival conditions for 15 days after the radiation session to evaluate collagen neosynthesis by fibroblasts and any vascular changes ( @entity2280 ). After irradiation the basal cell proliferation was reduced by approximately 50%. Extensive vasodilation occurred with altered capillary permeability accompanied by decreased @entity2280 transmembrane protein expression. Collagen synthesis and @entity1192 secretion were increased. @entity69207 significantly reduced capillary alterations, restored @entity2280 expression as well as epithelial cell proliferation and significantly decreased collagen synthesis and @entity1192 expression. With this ex vivo @entity1 skin model we confirmed the main modifications induced by radiotherapy as previously described in animal models: decreased basal cell proliferation and endothelial cell alterations and increased collagen synthesis by fibroblasts, probably under the influence of @entity1192 . The effect of @entity69207 emulsion on these histological and biochemical parameters may support its clinical efficacy.

Histochemical and biochemical modifications induced by experimental irradiation of @entity1 skin maintained in survival conditions and modulation by application of an emulsion containing XXXX .

@entity1 :: ('9606', 'Species') :: ['human'] @entity650 :: ('3569', 'Gene') :: ['IL-6'] @entity24924 :: ('MESH:C009546', 'Chemical') :: ['trolamine'] @entity4 :: ('MESH:D004487', 'Disease') :: ['edema'] @entity69207 :: ('MESH:C423724', 'Chemical') :: ['Biafine'] @entity1192 :: ('3552', 'Gene') :: ['IL-1', 'interleukin (IL)-1alpha'] @entity272 :: ('MESH:D012871', 'Disease') :: ['skin disorders'] @entity2280 :: ('947', 'Gene') :: ['CD34']
@entity24924 :: (MESH:C009546,Chemical) :: ['trolamine']
