BACKGROUND: Products cryopreserved with @entity3733 ( @entity3733 ) in @entity8 ( @entity8 ) often cause many adverse effects during their infusion (major cardiovascular events, @entity1353     even @entity204 ). These are especially frequent in pediatric @entity1 . We tested if a fully automated and closed wash procedure (Sepax S-100, Biosafe) allowed us to maintain the absolute @entity2280 + cell number, cell viability, and engraftment potential, decreasing the untoward reactions. STUDY DESIGN AND METHODS: Forty-six washes of @entity3733 cryopreserved peripheral blood @entity1583 ( @entity1583 ) apheresis were studied. Blood aliquots were taken both after thawing and after washing to assess the total nucleated and @entity2280 + cell counts, as well as cell viability. The washed products were infused in 26 autologous SCTs (ASCTs). Results were compared with the 53 previous SCTs performed without @entity3733 removal. RESULTS: After washing there were no significant differences between the pre- and postwashing @entity2280 + cell counts (p=0.08) or viability (p=0.68). No significant differences were observed between washed and nonwashed infusions in relation to the day of the neutrophil (p=0.46) and platelet (p=0.26) engraftment. One adverse event, @entity490 , occurred during the washed cells infusions. When compared with the 14 untoward reactions that took place during the nonwashed @entity1583 infusions, significance was reached (p=0.00043). CONCLUSIONS: The automatic method described is effective in terms of @entity2280 + cell recovery and viability in ASCT. Moreover, Sepax decreased significantly the untoward reactions during the infusion.

An automatic wash method for XXXX removal in autologous hematopoietic stem cell transplantation decreases the adverse effects related to infusion.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity8 :: ('MESH:D007674', 'Disease') :: ['SCT', 'stem cell transplant'] @entity1353 :: ('MESH:D004417', 'Disease') :: ['dyspnea'] @entity3733 :: ('MESH:D004121', 'Chemical') :: ['dimethyl sulfoxide', 'DMSO'] @entity1583 :: ('MESH:D019337', 'Disease') :: ['hematopoietic progenitor', 'HP'] @entity490 :: ('MESH:D015746', 'Disease') :: ['abdominal pain'] @entity2280 :: ('947', 'Gene') :: ['CD34'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death']
@entity3733 :: (MESH:D004121,Chemical) :: ['dimethyl sulfoxide']
