Although adenoviruses offer several potential advantages as gene transfer vectors, some @entity1583 cells, particularly lymphoid cells, are considered relatively resistant to adenovirus-mediated gene transfer. To examine the role of adenovirus-mediated gene transfer in the @entity706 ( @entity1582 ), we used E1- and E3-deleted adenoviral vectors to infect @entity1582 and @entity706 cell lines and subsequently primary bone marrow plasma cells and lymphocytes from @entity1 with @entity1582 . Adenoviral vectors expressing LacZ or luciferase (AdCA18) reporter genes were used initially. Subsequently, we studied adenoviral vectors expressing genes of potential value in therapeutic immunomodulation, i.e., @entity7938 (AdB7-1) and @entity1199 (AdIL-2). A @entity1 plasma cell line (OCI-My5) infected with LacZ or AdB7-1 vectors expressed the corresponding gene product in 95% and 85% of exposed cells, respectively. Time course experiments indicated that maximum expression of adenoviral transgenes in plasma cells was reached 3 days after @entity281 . @entity1199 was detected in the supernatant of AdIL-2-infected plasma cells, was functional, and could be detected for at least 30 days after @entity281 . In contrast, three @entity706 cell lines (OCI-Ly2, OCI-Ly13.2, and OCI-Ly17) were significantly less sensitive to @entity9504 , with relatively low efficiencies of gene transfer even using high adenoviral titers: Surface @entity7938 expression (13-25% of infected cells) and positive LacZ staining (0-5% of infected cells). Indeed, expression of luciferase was 96-168 times higher in AdCA18-infected OCI-My5 cells than in the OCI-Ly2 @entity706 cell line. Similar patterns were observed in primary plasma cells and lymphocytes from 19 @entity1582 @entity1 bone marrow samples. After @entity281 with AdB7-1, increased levels of @entity7938 expression on @entity3053 bright bone marrow plasma cells were observed in 84% of @entity1 , with a 33% average increase in the number of plasma cells expressing @entity7938 . In contrast, although increased @entity7938 expression was also detected on AdB7-1-infected @entity1075 + B lymphocytes from 63% of the @entity1582 @entity1 , an average of only 14% of the infected lymphocytes demonstrated increased expression of @entity7938 . Circulating T lymphocytes could not be transduced with AdB7-1. The relative resistance of B and T lymphocytes to adenovirus-mediated gene transfer warrants further investigation. Adenoviral vectors can efficiently infect malignant plasma cells and may be useful vehicles for therapeutic gene transfer.

Efficient adenovirus-mediated gene expression in malignant XXXX plasma cells: relative lymphoid cell resistance.

@entity1 :: ('9606', 'Species') :: ['patients', 'human', 'patient'] @entity9504 :: ('MESH:D000257', 'Disease') :: ['adenovirus infection'] @entity706 :: ('MESH:D008223', 'Disease') :: ['lymphoma', 'lymphoid malignancy multiple myeloma'] @entity1075 :: ('930', 'Gene') :: ['CD19'] @entity7938 :: ('941', 'Gene') :: ['CD80'] @entity1582 :: ('MESH:D009101', 'Disease') :: ['myeloma', 'MM'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity3053 :: ('952', 'Gene') :: ['CD38'] @entity1583 :: ('MESH:D019337', 'Disease') :: ['hematopoietic'] @entity1199 :: ('3558', 'Gene') :: ['interleukin-2', 'IL-2']
@entity1 :: (9606,Species) :: ['human']
