OBJECTIVE: This study aimed at describing one actinomycete strain E65 that was isolated from the water of Mellah Lake in El Kala, North-East of Algeria that produces a non-polyenic antifungal. MATERIALS AND METHODS: Actinomycetes were isolated from Mellah Lake water and screened for antimicrobial activity. Antimicrobial assays were performed on ISP2 agar. The taxonomic position of the strain E65 was determined regarding phenotypic and 16S DNA sequences features. Time course of antifungal metabolites production was evaluated against @entity5132 on ISP2, ISP1 and GYEA broth. The active antifungal compound was extracted using @entity1044 and revealed by a thin layer of chromatography, chemical reagents, UV-visible and infrared spectroscopy. RESULTS: A total of 104 actinomycetes were isolated and screened for antimicrobial activity; 21 strains were active against @entity794 , @entity526 and @entity5132 . The strain E65 showed a high in vitro activity against @entity794 and @entity5132 and a good antifungal activity against a clinical @entity5132 resistant to @entity4895 . Its 16S rRNA sequence shared 99% similarity with the @entity13891  yatensis type strain within the @entity50468 subclade of the @entity13891 clade. It produced a non-polyenic antifungal, the IR spectrum of the antifungal extract corresponded to none of the antimicrobials compounds known to be produced by actinomycete of the @entity13891 clade. CONCLUSION: The wetlands of El Kala, Algeria are a potential source of bioactive actinomycete that deserves to be explored and exploited. The @entity13891 yatensis E65 strain isolated from Mellah Lake brackish water produces a remarkable antifungal compound which original non-polyenic structure warrants further characterization.

A non-polyenic antifungal produced by a XXXX  yatensis strain isolated from Mellah Lake in El Kala, North-East of Algeria.

@entity1044 :: ('MESH:D008752', 'Chemical') :: ['dichloromethane'] @entity794 :: ('1280', 'Species') :: ['Staphylococcus  aureus', 'S.  aureus'] @entity4895 :: ('MESH:D005437', 'Chemical') :: ['5-fluorocytosine'] @entity5132 :: ('5476', 'Species') :: ['C.  albicans strain', 'Candida  albicans', 'C.  albicans'] @entity13891 :: ('1912', 'Species') :: ['Streptomyces', 'S.  hygroscopicus', 'Streptomyces  hygroscopicus'] @entity50468 :: ('68280', 'Species') :: ['Streptomyces  violaceusniger'] @entity526 :: ('562', 'Species') :: ['Escherichia  coli']
@entity13891 :: (1912,Species) :: ['Streptomyces']
