UNASSIGNED: Little is known about the effect of @entity3089 (EGCG) on angiogenesis in adipocytes. We aimed to test the effect of EGCG on the expression of @entity1611 ( @entity1611 ) in adipocytes. The levels of @entity1611 secretion, the expression of @entity1611 message ribonucleic acid (mRNA) and @entity1611 protein in 3T3-L1 cells were measured by enzyme linked immunosorbent assay (ELISA), real time polymerase chain reaction (PCR), and immunofluorescence staining, respectively. The xCELLigence real time cell analysis system was used to study the growth and differentiation of 3T3-L1 preadipocytes. A coculture system was used to test the effects of 3T3-L1 cells on proliferation of @entity1 umbilical vein endothelial cells (HUVECs). The conditioned media derived from 3T3-L1 cells treated with or without EGCG was used to culture the HUVECs for a tube formation assay. Peroxisome proliferator-activated receptor y (PPARy) and @entity16509 ( @entity16509 ), two transcription factors related to both adipogenesis and angiogenesis, were examined to explore the potential mechanism. We found that all the three measurements of @entity1611 expression in adipocytes (mRNA, protein and secretion in media) were reduced after EGCG treatment. The growth of HUVECs co-cultured with 3T3-L1 cells was significantly increased and the conditioned media from EGCG treated 3T3-L1 adipocytes inhibited tube formation in HUVECs. Both PPARy and @entity16509 expression in adipocytes were decreased with EGCG treatment. In conclusion, findings from this study suggest that EGCG may inhibit angiogenesis by regulating @entity1611 expression and secretion in adipocytes.

Real Time Monitoring of Inhibition of Adipogenesis and Angiogenesis by XXXX in 3T3-L1 Adipocytes and @entity1 Umbilical Vein Endothelial Cells.

@entity1611 :: ('22339(Tax:10090)', 'Gene') :: ['vascular endothelial growth factor', 'VEGF'] @entity1 :: ('9606', 'Species') :: ['human'] @entity3089 :: ('MESH:C045651', 'Chemical') :: ['(-)-epigallocatechin-3-gallate'] @entity16509 :: ('12606(Tax:10090)', 'Gene') :: ['CCAAT/enhancer binding protein a', 'C/EBPa']
@entity3089 :: (MESH:C045651,Chemical) :: ['(-)-Epigallocatechin-3-Gallate']
