The potential @entity1370 of @entity1068 ( @entity167 ) was evaluated in primary cultures of @entity35 myocardial cells. @entity167 @entity1370 was assessed in the cells on the basis of cell morphology, @entity880 dehydrogenase (LDH) leakage, @entity4588 dehydrogenase (SDH) activity, and beating rates. Cells were treated with @entity167 at concentrations of 600, 800, and 1000 mg% for duration of 1, 4, and 24 h and then evaluated for @entity1370 . Vacuole formation occurred 1 h after exposure to @entity167 at 800 and 1000 mg%; by 4 h, cytosolic granular material appeared in these cells. Exposure for 24 h to all concentrations of @entity167 resulted in vacuole, granule, and pseudopod formation and loss of cross-striations. Significant LDH leakage occurred at 1 h and 4 h with 800 and 1000 mg% @entity167 . LDH release was significantly increased after 24 h with all concentrations. SDH activity was significantly depressed after 24 h with all concentrations of @entity167 . Beating rates were altered as early as 1 h after exposure to 800 and 1000 mg% @entity167 . After 24 h, those cells exposed to the highest concentrations of @entity167 were not beating at all. These data suggest that primary myocardial cell cultures may be used to assess the in vitro @entity1370 of @entity167 to the myocardial cell.

@entity1068 toxicity in primary cultures of XXXX myocardial cells.

@entity167 :: ('CHEBI:16236', 'Chemical') :: ['EtOH'] @entity4588 :: ('MESH:D019802', 'Chemical') :: ['succinate'] @entity1370 :: ('MESH:D066126', 'Disease') :: ['cardiotoxicity'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity1068 :: ('MESH:D000431', 'Chemical') :: ['ethanol']
@entity35 :: (10116,Species) :: ['rat']
