UNASSIGNED: The purposes was to establish optimal modeling of single-chain antibody molecules based on similarity algorithm and seek the connecting peptides that had the minimal effect on the structure and bioactivity of the variable region of heavy chain (VH) and that of light chain ( @entity10389 ) in a single-chain antibody against @entity96 . After the Linker with different lengths (n=0~7) had been added into single chain fragment variable ( @entity10390 ), modeling of the overall sequences of VH, @entity10389 and @entity10390 were conducted respectively. Meanwhile, the peptide chain structure of ( @entity7507 )n was adopted for the connecting peptide. Then the spatial spherical shell layer alignment algorithm based on spherical polar coordinates was utilized for comparing the structural similarity of VH and @entity10389 before and after adding connecting peptide. Equally, in order to determine the stability of VH and @entity10389 , MATLAB was applied for analysis of the fore and aft distances and the diffusion radius. Indirect ELISA method was used to detect single-chain antibody immunological activity of Linker with different lengths. The @entity98 assay was utilized for the examination of the inhibition rate of single-chain antibody with different lengths of Linker to @entity96 cell. When n=4, the structural similarity between VH together with @entity10389 and their original ones was the highest. When n=3, the influence of connecting peptide on the stability of VH and @entity10389 was minimum. When n>3, the fore and aft distances changed little due to the increase and fold of the length of peptide chain. The results of ELISA detection showed that when n=4, affinity of single chain antibody to @entity96 cells was much higher. The @entity98 test also indicated that when n=4, the inhibition rate of the connecting peptide on @entity189 cell reached the highest, and that came second when n=3. When n=4, the structural stability and biological functions of anti- @entity157 single-chain antibody were both favorable. This study has provided a basis for the design and construction of single-chain antibody.

Optimization modeling of single-chain antibody against XXXX based on similarity algorithm.

@entity96 :: ('MESH:D008113', 'Disease') :: ['liver cancer'] @entity189 :: ('MESH:D002277', 'Disease') :: ['hepatoma carcinoma'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity10389 :: ('MESH:C536141', 'Disease') :: ['VL'] @entity157 :: ('MESH:D006528', 'Disease') :: ['hepatoma'] @entity10390 :: ('652070', 'Gene') :: ['ScFv'] @entity7507 :: ('p|SUB|G|4|S', 'ProteinMutation') :: ['Gly4Ser']
@entity157 :: (MESH:D006528,Disease) :: ['hepatoma']
