UNASSIGNED: Upon chronological aging, @entity1 skin undergoes structural and molecular modifications, especially at the level of type I collagen. This macromolecule is one of the main dermal structural proteins and presents several age-related alterations. It exhibits a triple helical structure and assembles itself to form fibrils and fibers. In addition, water plays an important role in stabilizing the collagen triple helix by forming @entity3884 -bonds between collagen residues. However, the influence of water on changes of dermal collagen fiber orientation with age has not been yet understood. Polarized-Fourier Transform Infrared (P-FTIR) imaging is an interesting biophotonic approach to determine in situ the orientation of type I collagen fibers, as we have recently shown by comparing skin samples of different ages. In this work, P-FTIR spectral imaging was performed on skin samples from two age groups (35- and 38-year-old on the one hand, 60- and 66-year-old on the other hand), and our analyses were focused on the effect of @entity4358 / @entity2768 substitution. Spectral data were processed with fuzzy C-means (FCM) clustering in order to distinguish different orientations of collagen fibers. We demonstrated that the orientation was altered with aging, and that @entity2768 treatment, affecting primarily highly bound water molecules, is more marked for the youngest skin samples. Collagen-bound water-related spectral markers were also highlighted. Our results suggest a weakening of water/collagen interactions with age. This non-destructive and label-free methodology allows us to understand better the importance of bound water in collagen fiber orientation alterations occurring with @entity272 . Obtaining such structural information could find benefits in dermatology as well as in cosmetics.

Investigating the relationship between changes in collagen fiber orientation during XXXX and collagen/water interactions by polarized-FTIR microimaging.

@entity1 :: ('9606', 'Species') :: ['human'] @entity3884 :: ('MESH:D006859', 'Chemical') :: ['hydrogen'] @entity4358 :: ('D014867', 'Chemical') :: ['H2O'] @entity2768 :: ('CHEBI:41981', 'Chemical') :: ['D2O'] @entity272 :: ('MESH:D012871', 'Disease') :: ['skin aging']
@entity272 :: (MESH:D012871,Disease) :: ['skin aging']
