BACKGROUND: Bone morphogenetic proteins (BMPs) are now being used as bone-graft substitutes to enhance spinal fusion. However, the large doses of @entity6861 required to induce a spinal fusion in @entity1 suggests that the delivery of these proteins should be improved. We used ex vivo adenoviral gene transfer to create @entity2095 -producing bone marrow cells, and these autologous cells were found to induce a posterolateral fusion of the spine in syngeneic @entity35 . METHODS: Intertransverse spinal arthrodesis (L4 and L5) was attempted in ten groups of Lewis @entity35 with 5 x 10 (6) @entity2095 -producing @entity35 bone marrow cells (Ad- @entity2095 cells), created through adenoviral gene transfer with @entity2097 -extracted demineralized bone matrix as a carrier (Group I); 5 x 10 (6) Ad- @entity2095 cells on a collagen sponge carrier (Group II); 10 micro g of recombinant @entity2095 (rhBMP-2) in a @entity2097 -extracted demineralized bone matrix carrier (Group III); 10 micro g of rhBMP-2 in a collagen sponge carrier (Group IV); autogenous iliac crest bone-grafting (Group V); 5 x 10 (6) beta-galactosidase-producing @entity35 bone marrow cells, created through adenoviral gene transfer with @entity2097 -extracted demineralized bone matrix as a carrier (Group VI); decortication of the transverse processes alone (Group VII); 5 x 10 (6) uninfected @entity35 bone marrow cells with a @entity2097 -extracted demineralized bone matrix carrier (Group VIII); @entity2097 -extracted demineralized bone matrix only (Group IX); or a collagen sponge alone (Group X). Each specimen underwent plain radiography, manual palpation, and histological analysis. RESULTS: All spines in Groups I and II ( @entity2095 -producing bone marrow cells) and all spines in Groups III and IV were fused at four weeks postoperatively. In contrast, none of the spines in the other groups had fused at a minimum of eight weeks after implantation. Histological analysis of the specimens revealed that the spines that had received @entity2095 -producing bone marrow cells (Groups I and II) were filled with coarse trabecular bone postoperatively, whereas those that had received rhBMP-2 (Groups III and IV) were filled with thin, lace-like trabecular bone. All of the other spines, including those that had been treated with autogenous iliac crest bone-grafting (Group V), produced little or no new bone. CONCLUSION: @entity2095 -producing bone marrow cells, created by adenoviral gene transfer, produce sufficient @entity6861 to induce an intertransverse fusion in the @entity35 spine model.

Effect of regional gene therapy with bone morphogenetic protein-2-producing bone marrow cells on spinal fusion in XXXX .

@entity1 :: ('9606', 'Species') :: ['humans'] @entity2097 :: ('MESH:D019791', 'Chemical') :: ['guanidine hydrochloride'] @entity2095 :: ('29373(Tax:10116)', 'Gene') :: ['BMP-2'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity6861 :: ('649', 'Gene') :: ['BMP']
@entity35 :: (10116,Species) :: ['rats']
