The ability of the immune system to recognize structurally altered, amplified or aberrantly expressed proteins can be used to identify molecules of etiologic relevance to @entity5 and to define targets for @entity5 immunotherapy. In the current study, ninety-four distinct antigens reactive with serum IgG from @entity0 @entity1 were identified by immunoscreening @entity0 -derived cDNA expression libraries (SEREX). A serological profile was generated for each antigen on the basis of reactivity with allogeneic sera from normal individuals and @entity5 @entity1 , and mRNA expression profiles for coding sequences were assembled based upon the tissue distribution of expressed sequence tags, Northern blots and real-time RT-PCR. Forty antigens reacted exclusively with sera from @entity5 @entity1 . These included well-characterized @entity5 antigens, e.g. @entity5840 , @entity43894 , @entity16051 , @entity1722 and @entity876 , as well as newly-defined @entity0 antigens, e.g. @entity31569 , @entity70383 , @entity5 protein D52 and MAGE D, and novel gene products, e.g. @entity70384 , @entity70385 , @entity29145 , and @entity70386 . With regard to expression profiles, two of the novel gene products, @entity70384 and @entity29145 , were characterized by a high level of testicular mRNA expression, and were overexpressed in 60% and 90% of @entity0 , respectively. In addition, mRNA encoding @entity5 protein D52 was overexpressed in 60% of @entity0 specimens, while transcripts encoding @entity26946 signal adaptor protein were downregulated in 70% of these cases. This study adds to the growing list of @entity0 antigens defined by SEREX and to the ultimate objective of identifying the complete repertoire of immunogenic gene products in @entity1 @entity5 (the @entity5 immunome).

Humoral immunity to @entity1 XXXX : antigen definition and quantitative analysis of mRNA expression.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity70386 :: ('92335', 'Gene') :: ['NY-BR-96'] @entity31569 :: ('3796', 'Gene') :: ['kinesin 2'] @entity29145 :: ('151648', 'Gene') :: ['NY-BR-85'] @entity26946 :: ('10818', 'Gene') :: ['SNT-1'] @entity0 :: ('MESH:D001943', 'Disease') :: ['breast cancers', 'breast cancer'] @entity1722 :: ('2064', 'Gene') :: ['Her2neu'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer'] @entity43894 :: ('4105', 'Gene') :: ['MAGE-6'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity70384 :: ('56992', 'Gene') :: ['NY-BR-62'] @entity70385 :: ('54680', 'Gene') :: ['NY-BR-75'] @entity70383 :: ('7110', 'Gene') :: ['TATA element modulatory factor 1'] @entity16051 :: ('246100', 'Gene') :: ['NY-ESO-1'] @entity5840 :: ('4102', 'Gene') :: ['MAGE-3']
@entity0 :: (MESH:D001943,Disease) :: ['breast cancer']
