UNASSIGNED: @entity3494 (As) @entity137 is a global health problem, affecting millions of @entity1 . Exposure to @entity3494 , mostly via drinking water, has been associated with @entity5 of skin, lungs, and blood, in addition to several kinds of @entity272 . The present study focused on the effect of @entity4447 ( @entity9188 ) on normal skin fibroblast cells. Specifically, the effect of @entity9188 on ROS generation and oxidative stress was investigated. Proteins involved in the DNA damage signaling pathway and cell cycle were also studied. @entity9188 induced the generation of intracellular ROS. Immunohistochemistry analysis revealed a dose-dependent increase in the number of @entity20821 -positive cells, an indication of oxidative stress. Cell cycle analysis by flow cytometry demonstrated that @entity9188 caused a significant percentage of cells to accumulate in the G0/G1 phase with a concomitant reduction in the S phase. Increases in the activated forms of DNA damage signaling proteins, ATM and ATR, and their effector molecules, @entity9516 and @entity876 , were also observed. In addition, expression of oncogene @entity3544 was also increased. The study shows that exposure of normal skin fibroblast cells to @entity9188 could lead to cell cycle arrest through ATM/ATR and DNA damage signaling pathways. In conclusion, we report here that @entity4447 increases cellular oxidative stress leading to shift in cell cycle and leads to DNA damage through ATM/ATR and the @entity17342 -dependent signaling pathway.

@entity4447 induces ROS activity and DNA damage, leading to G0/G1 extension in skin fibroblasts through the ATM-ATR-associated XXXX pathway.

@entity1 :: ('9606', 'Species') :: ['people'] @entity4447 :: ('MESH:C006632', 'Chemical') :: ['arsenic trioxide'] @entity20821 :: ('MESH:C067134', 'Chemical') :: ['8-OHdG'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity'] @entity9188 :: ('CHEBI:30621', 'Chemical') :: ['As2O3'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity3544 :: ('644914', 'Gene') :: ['p21'] @entity876 :: ('7157', 'Gene') :: ['p53'] @entity17342 :: ('1119', 'Gene') :: ['CHK'] @entity272 :: ('MESH:D012871', 'Disease') :: ['skin lesions'] @entity3494 :: ('MESH:D001151', 'Chemical') :: ['arsenic', 'Arsenic'] @entity9516 :: ('11200', 'Gene') :: ['Chk2']
@entity17342 :: (1119,Gene) :: ['Chk']
