Effects of @entity864 ( @entity865 ) and the @entity99 ( @entity99 ) @entity12880 , @entity7325 ( @entity3824 ), @entity5248 ( @entity5248 ) and @entity5247 ( @entity5247 ) were studied in 2 gap junctional intercellular communication (GJIC) systems, metabolic cooperation in V79 cells and microinjection/dye transfer in @entity329 embryo (SHE) cells and V79 cells. @entity865 inhibited GJIC in both systems but was considerably more potent in V79 cells. SHE cells showed a rapid and transient inhibition of GJIC after exposure to @entity99 , with maximal inhibition occurring at 5-15 min. The transient inhibition could be caused by metabolization of the compounds. @entity12880 and @entity5247 produced strong inhibition of metabolic cooperation in V79 cells at high concentrations, while the effect of @entity3824 and @entity5248 was lower. However, @entity5248 , @entity5247 and @entity12880 strongly inhibited dye transfer in V79 cells after a 30 min exposure. @entity12880 also showed a dose- and time-dependent effect on dye transfer in V79 cells. The phosphorylation status of the gap junction protein connexin43 (Cx43) changed minimally in SHE cells after exposure to @entity865 or @entity99 . Cx43 from V79 cells was strongly affected by @entity865 , but not by @entity99 . Immunofluorescence of Cx43 disappeared in both cell types when they were exposed to @entity865 and @entity5247 , but not to the other @entity99 . Thus, there is no direct correlation between the inhibition of GJIC and changes in the phosphorylation status of Cx43 or the appearance of Cx43 in immunofluorescence experiments. The discrepancies may partly be explained by binding of accessory proteins to Cx43. We point out sequences that may be involved in such binding.

Effects of peroxisome proliferators and XXXX on intercellular communication and connexin43 in two hamster fibroblast systems.

@entity5247 :: ('MESH:C016599', 'Chemical') :: ['MEHP', 'mono(2-ethylhexyl)phthalate'] @entity99 :: ('MESH:D056486', 'Disease') :: ['HPPs', 'hepatic peroxisome proliferators'] @entity12880 :: ('MESH:D002994', 'Chemical') :: ['clofibrate', 'Clofibrate'] @entity7325 :: ('MESH:D002992', 'Chemical') :: ['methyl clofenapate'] @entity5248 :: ('MESH:D004051', 'Chemical') :: ['DEHP', 'di(2-ethylhexyl)phthalate'] @entity3824 :: ('CHEBI:50099', 'Chemical') :: ['MCP'] @entity865 :: ('CHEBI:61432', 'Chemical') :: ['TPA'] @entity329 :: ('10036', 'Species') :: ['Syrian hamster'] @entity864 :: ('MESH:C554292|MESH:D013755', 'Chemical') :: ['12-O-tetradecanoyl phorbol 13-acetate']
@entity864 :: (MESH:C554292|MESH:D013755,Chemical) :: ['12-O-tetradecanoyl phorbol-13-acetate']
