UNASSIGNED: The aim of this study was to examine the effects of compound Radix Sophorae Flavescentis on the proliferation of @entity418 TE-8 cells and to elucidate the mechanisms involved. For this purpose, we incubated TE-8 cells in medium containing various concentrations (0, 0.0125, 0.025, 0.05, 0.1, 0.2, 0.4 and 0.8 mg/ml) of the compound Radix Sophorae Flavescentis injection and its effects on the proliferation of TE-8 cells were examined by @entity2454 ( @entity98 ) assay. In addition, we observed the morphological changes and measured the expression levels of apoptosis-related genes ( @entity522 , @entity588 and @entity589 ) in the cells treated with different doses of the compound (low-dose group, 0.05 mg/ml; medium-dose group, 0.2 mg/ml; and high-dose group, 0.8 ng/ml) by reverse transcription-quantitative PCR (RT-qPCR). The apoptotic index of the @entity5 cells treated with different doses of the compound was determined by TUNEL assay. Our results revealed that compared with the control group (untreated cells), the proliferation of the @entity5 cells treated with the compound was significantly inhibited (P <=0.05); the inhibition of the proliferation of the @entity5 cells occured in a dose-dependent manner. Compared with the control group, the apoptotic rate of the cells in the low-dose, medium-dose and high-dose groups increased significantly (P<0.05) in a dose-dependent manner. In addition, compared with the control group, the mRNA expression of @entity522 and @entity589 increased significantly in the cells treated with the compound. However, the mRNA expression of @entity588 markedly decreased (P<0.05). With the gradual increase in the drug concentration, the mRNA expression levels of @entity522 , @entity588 and @entity589 in the @entity5 cells were altered in a dose-dependent manner. In conclusion, our data demonstrate that compound Radix Sophorae Flavescentis injection significantly enhances the expression of pro-apoptotic genes in @entity418 TE-8 cells by increasing apoptosis and inhibiting cell proliferation. Thus, this study provides a theoretical basis for the clinical treatment of @entity418 .

Compound Radix Sophorae Flavescentis exerts antitumor effects by inhibiting the proliferation and inducing the apoptosis of XXXX TE-8 cells.

@entity522 :: ('836', 'Gene') :: ['caspase-3'] @entity588 :: ('596', 'Gene') :: ['Bcl-2'] @entity589 :: ('581', 'Gene') :: ['Bax'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity2454 :: ('MESH:C022616', 'Chemical') :: ['3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide'] @entity418 :: ('MESH:D004938', 'Disease') :: ['esophageal carcinoma']
@entity418 :: (MESH:D004938,Disease) :: ['esophageal carcinoma']
