The present study was designed to examine whether @entity7784 ( @entity8393 ) inhibits the production of basal or stimulated endothelin (ET)-1 by platelet-derived growth factor (PDGF)-BB or @entity11428 ( @entity11428 ), and DNA synthesis in cultured @entity291 mesangial cells. PDGF-BB and @entity11428 stimulated ET-1 secretion in a dose-dependent fashion in these cells. @entity8393 (10-100 microM) exhibited dose-related inhibition of PDGF-BB- and @entity11428 -stimulated ET-1 secretion. @entity8393 had no inhibitory effects on basal ET-1 secretion in these cells. Moreover, 50 microM @entity8393 significantly attenuated PDGF-BB- and @entity11428 -stimulated @entity665 incorporation into mesangial cells. Receptor-binding experiments showed that @entity8393 competitively inhibited 125I-PDGF-BB or 125I- @entity11428 binding to mesangial cell surface receptors. Scatchard analysis for PDGF-BB receptor or @entity11428 receptor revealed a linear regression fit and one binding site. Pretreatment with 50 microM @entity8393 suppressed the number of maximum binding sites, but did not affect the Kd values. These results indicate that @entity8393 potentially inhibits mesangial cell ET-1 production, when stimulated by PDGF-BB or @entity11428 . This inhibitory effect of @entity8393 could be related to the attenuation of mesangial cell proliferation via inhibition of the binding of PDGF-BB or @entity11428 to their receptors due to alteration of the physicochemical characteristics of the cell membrane.

@entity7784 inhibits mitogen-induced endothelin-1 production and DNA synthesis in cultured XXXX mesangial cells.

@entity7784 :: ('MESH:D015118', 'Chemical') :: ['eicosapentaenoic acid'] @entity8393 :: ('CHEBI:28364', 'Chemical') :: ['EPA'] @entity665 :: ('CHEBI:53526', 'Chemical') :: ['[3H]thymidine'] @entity11428 :: ('521832(Tax:9913)', 'Gene') :: ['epidermal growth factor', 'EGF'] @entity291 :: ('9913', 'Species') :: ['bovine']
@entity291 :: (9913,Species) :: ['bovine']
