UNASSIGNED: The aim of the present study was to characterise receptors for LH and FSH (LHR and @entity64018 , respectively) and aromatase in epididymal and testicular tissue from stallions of different ages (prepubertal, young, mature and old). Gene and protein expression were assessed by real-time quantitative polymerase chain reaction (real-time qPCR), immunohistochemistry and multiple immunofluorescence labelling. There were no differences in LHR mRNA expression in epididymal and testicular parenchyma in stallions of different age. In contrast, expression of @entity64018 and @entity22362 in caput, corpus and cauda epididymis and in testicular parenchyma increased with age (P < 0.001). Immunolabelling for LHR, @entity64018 and aromatase was influenced by puberty. In postpubertal stallions, positive staining for LHR and aromatase was detected in Leydig cells, whereas protein expression of @entity64018 was present in Sertoli cells and primary spermatocytes. In prepubertal colts, staining for LHR, @entity64018 and aromatase was detected in seminiferous tubules. In epididymal tissue, aromatase was present in the cauda epididymis only, regardless of age. In conclusion, the results highlight the significance of gonadotropin action and @entity2931 production for the maturation of male reproductive tissue in the @entity1641 . The presence of @entity64018 in the seminiferous tubules suggests effects of FSH on spermatogenesis in this species. The importance of @entity2931 production for maintenance of testicular function in stallions was confirmed.

Effect of stallion age on the expression of LH and FSH receptors and aromatase P450 in XXXX male reproductive tissues.

@entity22362 :: ('100009712(Tax:9796)', 'Gene') :: ['CYP19A1'] @entity1641 :: ('9796', 'Species') :: ['horse'] @entity2931 :: ('CHEBI:50114', 'Chemical') :: ['oestrogen'] @entity64018 :: ('100052957(Tax:9796)', 'Gene') :: ['FSHR']
@entity1641 :: (9796,Species) :: ['equine']
