UNASSIGNED: Standard tissue preparation for light and @entity4653 ( @entity4653 ) uses @entity1068 as a dehydrating agent but that can also dissolve @entity165 crystals (CC) leaving behind empty tissue imprints or "clefts". @entity165 crystals may contribute to @entity397 by their sharp tips that can tear membranes and trigger @entity32 . Therefore, use of @entity1068 in tissue processing can mask the pathological role of CC. Here we evaluated the amount of @entity165 dissolved from CC with single and complete series of standard graded @entity1068 concentrations (25-100%) used in tissue preparation. Also, solubility of CC in @entity1068 at physiological levels was measured. Furthermore, we compared the effect of @entity1068 on CC in fresh @entity1 @entity731 to matched segments dehydrated using vacuum (-1 atm, 12h). Tissue crystal density ranging from 0 to +3 was measured semi-quantitatively by @entity4653 . For CC exposed to 25% and 100% @entity1068 for 10 min each, 0.38% and 95% of CC were dissolved respectively. Also, increase in CC solubility was significant at physiological levels of @entity1068 (0.16%) compared to water (43.4        18.0 ng/mL vs. 30.9        13.9 ng/mL; p   <   0.05). We speculate that this could represent a potential mechanism of cardio-protective effects of @entity285 consumption. In @entity731 , CC density was lower in @entity1068 vs. saline treatment (+1.2 vs. +2.8; P   <   0.01) with visible dissolving noted by @entity4653 . @entity1068 has been used for centuries in tissue preparation for microscopy. Here we demonstrate how current tissue preparation methods greatly alter histological findings with @entity4653 by masking the potential mechanism of @entity397 . Microsc. Res. Tech. 78:969-974, 2015.    2015 Wiley Periodicals, Inc.

Role of XXXX crystals in @entity731 is unmasked by altering tissue preparation methods.

@entity1 :: ('9606', 'Species') :: ['human'] @entity32 :: ('MESH:D007249', 'Disease') :: ['inflammation'] @entity397 :: ('MESH:D012421', 'Disease') :: ['plaque rupture'] @entity731 :: ('MESH:D050197', 'Disease') :: ['atherosclerotic plaques'] @entity285 :: ('D000431', 'Chemical') :: ['alcohol'] @entity1068 :: ('MESH:D000431', 'Chemical') :: ['Ethanol', 'ethanol'] @entity165 :: ('MESH:D002784', 'Chemical') :: ['Cholesterol', 'cholesterol'] @entity4653 :: ('MESH:D004401', 'Disease') :: ['SEM', 'scanning electron microscopy']
@entity165 :: (MESH:D002784,Chemical) :: ['cholesterol']
