Osteoprogenitor cells in the @entity1 bone @entity236 can be induced to differentiate into osteoblasts under stimulation with hormonal and local factors. We previously showed that @entity1 bone marrow stromal (HBMS) cells respond to @entity619 and @entity560 by expressing several osteoblastic markers. In this study, we investigated the effects and interactions of local factors ( @entity1841 and @entity6474 ) on HBMS cell proliferation and differentiation in short-term and long-term cultures. We found that rhTGF-beta2 increased DNA content and stimulated type I collagen synthesis, but inhibited @entity731 activity and mRNA levels, osteocalcin production, and mineralization of the matrix formed by HBMS cells. In contrast, rhBMP-2 increased @entity731 activity and mRNA levels, osteocalcin levels and @entity460 deposition in the extracellular matrix without affecting type I collagen synthesis and mRNA levels, showing that rhBMP-2 and rhTGF-beta2 regulate differentially HBMS cells. Co-treatment with rhBMP-2 and rhTGF-beta2 led to intermediate effects on HBMS cell proliferation and differentiation markers. rhTGF-beta2 attenuated the stimulatory effect of rhBMP-2 on osteocalcin levels, and @entity731 activity and mRNA levels, whereas rhBMP-2 reduced the rhTGF-beta2-enhanced DNA synthesis and type I collagen synthesis. We also investigated the effects of sequential treatments with rhBMP-2 and rhTGF-beta2 on HBMS cell differentiation in long-term culture. A transient (9 days) treatment with rhBMP-2 abolished the rhTGF-beta2 response of HBMS cells on @entity731 activity. In contrast, a transient (10 days) treatment with rhTGF-beta2 did not influence the subsequent rhBMP-2 action on HBMS cell differentiation. The data show that @entity6474 acts by increasing HBMS cell proliferation and type I collagen synthesis whereas @entity1841 acts by promoting HBMS cell differentiation. These observations suggest that @entity6474 and @entity1841 may act in a sequential manner at different stages to promote @entity1 bone marrow stromal cell differentiation towards the osteoblast phenotype.

Bone morphogenetic protein-2 and transforming growth factor-beta2 interact to modulate XXXX bone marrow stromal cell proliferation and differentiation.

@entity1 :: ('9606', 'Species') :: ['human'] @entity1841 :: ('650', 'Gene') :: ['BMP-2'] @entity731 :: ('MESH:D050197', 'Disease') :: ['ALP'] @entity619 :: ('MESH:D003907', 'Chemical') :: ['dexamethasone'] @entity460 :: ('MESH:D002118', 'Chemical') :: ['calcium'] @entity6474 :: ('7042', 'Gene') :: ['TGF-beta2'] @entity236 :: ('MESH:D001855', 'Disease') :: ['marrow stroma'] @entity560 :: ('MESH:D014807', 'Chemical') :: ['vitamin D']
@entity1 :: (9606,Species) :: ['human']
