There has been accumulating histological observation of @entity2909 and @entity1822 of the external soft tissue regarding their differential diagnosis. The definitive diagnostic tools have not been established, however, nor have the pathological mechanisms of cell proliferation in these @entity5 been clarified. Herein, expression of the @entity1364 -dependent kinase inhibitors (CKIs), @entity994 , @entity993 and @entity8755 and their associated kinase activities were examined in 61 cases of soft tissue smooth @entity11090 . Immunohistochemical staining showed that all 3 inhibitor proteins were expressed in all cases of @entity2909 and @entity1822 , but that the mean values of their labeling indices (LIs) were higher in the cases of @entity1822 . In addition, the LIs of @entity994 and @entity993 were inversely correlated in total cases. Immunoblotting revealed that these proteins are expressed at higher levels in @entity5 , in particular, in @entity1822 . When CKIs were immunoprecipitated from tissue extracts, @entity1364 /cdk protein complexes associated with, at least, 1 CKI were detectable only in @entity5 tissues. Furthermore, @entity15308 or @entity8985 kinase activity manifested by these @entity1364 /cdk/CKI complexes (CKI-associated kinase activity) was detectable exclusively from @entity1822 , but not from @entity2909 . Among the cases of @entity1822 , @entity15308 activity was generally found associated either with @entity994 or @entity993 , but not both. Statistical analysis indicated that @entity994 - and @entity993 LIs are predictive of positive or negative clinical outcome, respectively. In conclusion, the participation of CKIs in active @entity1364 /cdk complexes in a reciprocal and redundant manner and subsequent CKI- associated kinase activity are the characteristic profiles of malignant phenotype in these @entity5 . Moreover, immunohistochemical detection of CKIs may provide a useful tool for evaluating @entity1 ' prognosis.

CDK-inhibitors-associated kinase activity: a possible determinant of malignant potential in smooth XXXX of the external soft tissue.

@entity1 :: ('9606', 'Species') :: ['patients'] @entity1364 :: ('5111', 'Gene') :: ['cyclin'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'tumors'] @entity993 :: ('10671', 'Gene') :: ['p27'] @entity8985 :: ('1019', 'Gene') :: ['cdk4'] @entity15308 :: ('1017', 'Gene') :: ['cdk2'] @entity1822 :: ('MESH:D007890', 'Disease') :: ['leiomyosarcoma'] @entity8755 :: ('1028', 'Gene') :: ['p57'] @entity11090 :: ('MESH:D019042', 'Disease') :: ['muscle tumors'] @entity2909 :: ('MESH:D007889', 'Disease') :: ['leiomyoma'] @entity994 :: ('1026', 'Gene') :: ['p21']
@entity11090 :: (MESH:D019042,Disease) :: ['muscle tumors']
