Spermatogenesis is a complex process closely associated with the phosphorylation-orchestrated cell cycle. Elucidating the phosphorylation-based regulations should advance our understanding of the underlying molecular mechanisms. Here we present an integrative study of phosphorylation events in the testis. Large-scale phosphoproteome profiling in the adult @entity19 testis identified 17,829 phosphorylation sites in 3955 phosphoproteins. Although only approximately half of the phosphorylation sites enriched by IMAC were also captured by @entity3759 , both the phosphoprotein data sets identified by the two methods significantly enriched the functional annotation of spermatogenesis. Thus, the phosphoproteome profiled in this study is a highly useful snapshot of the phosphorylation events in spermatogenesis. To further understand phosphoregulation in the testis, the site-specific kinase-substrate relations were computationally predicted for reconstructing kinase-substrate phosphorylation networks. A core sub-kinase-substrate phosphorylation networks among the spermatogenesis-related proteins was retrieved and analyzed to explore the phosphoregulation during spermatogenesis. Moreover, network-based analyses demonstrated that a number of protein kinases such as MAPKs, @entity17068 , and @entity16602 with statistically more site-specific kinase-substrate relations might have significantly higher activities and play an essential role in spermatogenesis, and the predictions were consistent with previous studies on the regulatory roles of these kinases. In particular, the analyses proposed that the activities of @entity68108 -like kinases (PLKs) might be dramatically higher, while the prediction was experimentally validated by detecting and comparing the phosphorylation levels of pT210, an indicator of @entity18591 activation, in testis and other tissues. Further experiments showed that the inhibition of @entity68108 -like kinases decreases cell proliferation by inducing G2/M cell cycle arrest. Taken together, this systematic study provides a global landscape of phosphoregulation in the testis, and should prove to be of value in future studies of spermatogenesis.

Systematic Analysis of the Phosphoproteome and Kinase-substrate Networks in the XXXX Testis.

@entity19 :: ('10090', 'Species') :: ['mouse'] @entity68108 :: ('CHEBI:39299', 'Chemical') :: ['POLO'] @entity18591 :: ('18817(Tax:10090)', 'Gene') :: ['PLK1'] @entity16602 :: ('12534(Tax:10090)', 'Gene') :: ['CDC2'] @entity17068 :: ('12566(Tax:10090)', 'Gene') :: ['CDK2'] @entity3759 :: ('CHEBI:32234', 'Chemical') :: ['TiO2']
@entity19 :: (10090,Species) :: ['Mouse']
