PURPOSE: The cytostatic substance @entity1138 ( @entity1139 ) is used in trabeculectomy to enhance the success rate in problematic cases and is usually dissolved immediately before application, but only approximately 1% of the substance is needed for treatment. The authors evaluated different methods of long-time storage of @entity1139 for a period of 6 months. METHODS: @entity1139 in concentrations of 0.02, 0.05, 0.1, 0.2, and 0.4 mg/mL was prepared at the local pharmaceutical department and stored at +22 degrees C (room temperature), +4 degrees C (refrigerator), -20 degrees C (freezer compartment), and -196 degrees C (liquid N2). The activity of @entity1139 was evaluated with a microagar diffusion method (bioassay) after 30 minutes, and 1, 3, 7, 14, 30, 90, and 180 days for the different concentrations and storage methods. RESULTS: There was no difference in the long-term stability of the investigated @entity1139 concentrations. Ninety percent of the initial activity was preserved after storage at +22 degrees C for 1 week, -20 degrees C for 1 month, or +4 degrees C for 3 months. At 6 months the activities were 16%, 48%, and 78% of the initial values, respectively. The activity of @entity1139 stored at -196 degrees C is not reduced after storage for 6 months. CONCLUSIONS: Dissolved @entity1139 can be stored in the refrigerator for up to 3 months without significant loss of activity. Storage at room temperature is not recommended. Costs of trabeculectomy can be reduced by storage of reconstituted solutions rather than dissolving @entity1139 before each @entity726 surgery.

Activity of dissolved XXXX after different methods of long-term storage.

@entity1139 :: ('CHEBI:27504', 'Chemical') :: ['MMC'] @entity1138 :: ('MESH:D016685', 'Chemical') :: ['mitomycin C'] @entity726 :: ('MESH:D005901', 'Disease') :: ['glaucoma']
@entity1138 :: (MESH:D016685,Chemical) :: ['mitomycin C']
