OBJECTIVE: To study the role of @entity273 ( @entity274 ) in promoting proliferation and transformation of @entity35 liver oval cell line WB-F344. METHODS: Culturing WB-F344 cells were stimulated directly by @entity274 . The effect of @entity274 in promoting proliferation of WB cells was investigated by using @entity98 colorimetric analysis and 3H-TdR incorporation liquid scintillation counter. The normal WB cells and the WB cells initiated with @entity2482 ( @entity2482 ) were both promoted by stimulating continuously with @entity274 of low concentration (800 nmol/L). The transformation effect was tested by morphologic observation, karyotype analysis, and anchorage-independent growth assay. RESULTS: Proliferation of WB cells was induced obviously by @entity274 of low concentration for only one time. The normal WB cells and the WB cells initiated with MNNG were transformed by action with @entity274 of low concentration continuously for 28 days and typical morphologic characters of transformed cells were observed. In karyotype analysis the cells chromosome number changed and the frequency of structure aberration raised dramatically. Otherwise the transformed cells could form clone on self-solid culture medium. CONCLUSIONS: The biological effects of @entity274 were related to the low dose concentration in promoting proliferation and transformation of liver oval cells indicating its important role in hepatocarcinogenesis. Therefore antioxidants should be able to provide a new clue in prevention and cure of @entity157 .

[Role of @entity273 in promoting proliferation and transformation of XXXX liver oval cell line WB-F344].

@entity2482 :: ('MESH:D008769', 'Chemical') :: ['MNNG', "N-methyl-N'-nitro-N-nitrosoguanidine"] @entity274 :: ('CHEBI:16240', 'Chemical') :: ['H2O2'] @entity273 :: ('MESH:D006861', 'Chemical') :: ['hydrogen peroxide'] @entity98 :: ('CHEBI:53233', 'Chemical') :: ['MTT'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity157 :: ('MESH:D006528', 'Disease') :: ['hepatoma']
@entity35 :: (10116,Species) :: ['rat']
