Through bioinformatics and experimental approaches, we have assigned the first biochemical property to a predicted protein product in the @entity1 genome as a new 14-3-3 binding protein. 14-3-3 client proteins represent a diverse group of regulatory molecules that often function as signaling integrators in response to various environmental cues and include proteins such as @entity1835 and Foxo. Using 14-3-3 as a probe in a yeast two-hybrid screen, we identified a novel 14-3-3 binding protein with unknown function, initially designated as clone 546. Confocal microscopy revealed that clone 546 localized to the nucleus of @entity1 cells. Additional studies show that the gene encoding clone 546 is expressed in many @entity1 tissues, including the thymus, as well as a number of @entity5 cell lines. The interaction of clone 546 with 14-3-3 was confirmed in @entity1 cells. Interestingly, this interaction was markedly enhanced by the expression of activated @entity584 , suggesting a phosphorylation dependent event. Mutational analysis was carried out to identify Ser479 as the predominant residue that mediates the clone 546/14-3-3 association. Phosphorylation of Ser479 by @entity584 further supports a critical role for @entity584 in regulation of the clone 546/14-3-3 interaction. On the basis of these findings, we named this undefined protein @entity60665 : Fourteen-three-three associated @entity584 Substrate.

Identification of XXXX as a novel 14-3-3-associated nuclear protein.

@entity1 :: ('9606', 'Species') :: ['mammalian', 'human'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity584 :: ('207', 'Gene') :: ['AKT/PKB', 'AKT', 'Akt/PKB'] @entity60665 :: ('55355', 'Gene') :: ['FAKTS'] @entity1835 :: ('572', 'Gene') :: ['Bad']
@entity60665 :: (55355,Gene) :: ['FAKTS']
