@entity14 ( @entity14 ) is the third most common @entity5 and the second leading cause of @entity5 in America. Nearly two thirds of newly diagnosed @entity14 cases include @entity1600 ( @entity1600 ) involvement, and @entity1600 @entity3 is one of the strongest negative prognostic factors for @entity14 . It is thought that @entity14 contain a small population of drug-resistant @entity14 -initiating cells (Co- @entity1775 ) that may be responsible for @entity5 recurrence. To evaluate the effects of the @entity1600 stromal cells on Co- @entity1775 , we established a unique xenoplant model using @entity14 cells isolated by enzymatic digestion from consented @entity1 specimens, HT-29 cells, HCA-7 cells, and @entity1600 stromal cell line HK cells. We found that HK cells and HK cell-conditioned media enhanced @entity14 formation and @entity5 angiogenesis. Cells expressing CD133(+) and the stromal cell-derived factor 1a (SDF-1a) receptor @entity436 were enriched in chemotherapeutic-resistant @entity14 cells. @entity436 (+) Co- @entity1775 isolated from @entity1 specimens are more tumorigenic than unsorted @entity5 cells. Furthermore, the inhibitors specific to HK cell-derived SDF-1a reduced @entity5 formation and @entity5 angiogenesis. Our results have demonstrated a role for Co- @entity1775 in @entity5 growth and defined the influence of @entity1600 stromal cells on Co- @entity1775 . We have identified a major Co-TIC/ @entity1600 microenvironment-specific mechanism for @entity14 resistance to chemotherapeutic agents and established experimental platforms for both in vitro and in vivo testing, indicating that SDF-1a and its receptor, @entity436 , may be targets for clinical therapy.

Lymph node stromal cells enhance drug-resistant colon cancer cell @entity5 formation through SDF-1a/ XXXX paracrine signaling.

@entity1 :: ('9606', 'Species') :: ['patient'] @entity1775 :: ('MESH:D020323', 'Disease') :: ['TICs'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer-related deaths', 'cancer', 'malignancy'] @entity1600 :: ('MESH:D014388', 'Disease') :: ['LN', 'lymph node'] @entity3 :: ('MESH:D009362', 'Disease') :: ['metastasis'] @entity14 :: ('MESH:D015179', 'Disease') :: ['Colorectal cancer', 'CRC', 'CRC tumor', 'CRC tumors'] @entity436 :: ('7852', 'Gene') :: ['CD133(+)CXCR4', 'CXCR4']
@entity436 :: (7852,Gene) :: ['CXCR4']
