UNASSIGNED: An increase of Enterobacteriaceae isolates with reduced susceptibility to @entity6944 (FEP) and @entity115 / @entity798 (AMC) has been observed in our area. The aim of this study was to characterize this antibiotic resistance phenotype and its molecular epidemiology. A total of 33 Enterobacteriaceae strains were studied. @entity68139 genes and their genetic environment were analyzed by polymerase chain reaction (PCR) and sequencing. Plasmids were transferred by conjugation and/or transformation and classified using PCR-based inc/rep typing and IncF subtyping. @entity526 isolates were typed by phylogroup, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. Outer membrane proteins were studied by @entity3208 - @entity2975 gel electrophoresis and expression of @entity68139 genes by reverse transcription-PCR. FEP minimum inhibitory concentration yielded values of 1-16   mg/L. Twenty-nine (87.9%) isolates produced @entity40225 , of which 24 (82.7%) were located in class 1 integron, and 9 (27.3%) produced @entity18585 . Among the 24 @entity526 @entity40225 -producers, PFGE revealed two main clusters: one belonged to C-ST88 and the other to B23-ST131. Thirteen plasmids containing @entity68139 were transferred, nine belonged to IncF replicon (4 F2:A1:B-, 2 F1:A1:B1, 1 F1:A2:B-, 1 F18:A2:B1, 1 F5:A-:B1) and four were nontypeable. In conclusion, reduced susceptibility to FEP was mostly due to @entity40225 beta-lactamase. In @entity526 , this increase is mainly due to the dissemination of two clones, which have captured different IncF plasmids. Among non- @entity526 strains, five isolates produced @entity40225 and one isolate produced only @entity18585 .

Reduced Susceptibility to Cefepime in Clinical Isolates of Enterobacteriaceae Producing XXXX Beta-Lactamase.

@entity18585 :: ('2716540(Tax:562)', 'Gene') :: ['TEM-1'] @entity115 :: ('MESH:D000658', 'Chemical') :: ['amoxicillin'] @entity3208 :: ('MESH:D012967', 'Chemical') :: ['sodium dodecylsulfate'] @entity6944 :: ('MESH:C043266', 'Chemical') :: ['cefepime'] @entity68139 :: ('17035637', 'Gene') :: ['blaOXA-1'] @entity798 :: ('MESH:D019818', 'Chemical') :: ['clavulanate'] @entity2975 :: ('CHEBI:51135', 'Chemical') :: ['polyacrylamide'] @entity40225 :: ('8319151(Tax:562)', 'Gene') :: ['OXA-1'] @entity526 :: ('562', 'Species') :: ['Escherichia coli', 'E. coli']
@entity40225 :: (8319151(Tax:562),Gene) :: ['OXA-1']
