A multitude of stem cell types has been extensively studied and used for myocardial regenerative therapy. Amongst these endothelial progenitor cells form a promising source. In our present study, we investigated a one-staged approach for isolation and application of autologous endothelial progenitor cells in a @entity1707 model of @entity583 . Endothelial progenitor cell isolation was performed by immediately preprocedural @entity236 and consecutive positive selection by aptamer-based magnetic cell sorting. Animals were divided in three groups receiving endothelial progenitor cells, saline, or no intramyocardial injection respectively. Postprocedural follow-up consisted of weekly echocardiographic evaluations. Postmortem histological analysis after four weeks focused on detection of transplanted @entity13214 -positive endothelial progenitor cells and neovascularization within the infarcted @entity1562 . A significant difference in left ventricular ejection fraction could not be shown between the three groups. @entity13214 -stained endothelial progenitor cells could be found in the endothelial progenitor cells transplanted group, although detection was scarce. Large-sized capillaries were found to be significantly more in endothelial progenitor cells treated @entity1562 . The one-stage approach of endothelial progenitor cells isolation and application presented herein offers a new therapeutic concept. Even though a beneficial impact on myocardial function could not be assessed, increased neovascularization may indicate positive effects on remodeling processes. Being able to harvest endothelial progenitor cells right before application provides a wider scope of action in urgent cases.

One-staged aptamer-based isolation and application of endothelial progenitor cells in a porcine XXXX model.

@entity1707 :: ('9823', 'Species') :: ['pig'] @entity583 :: ('MESH:D009203', 'Disease') :: ['myocardial infarction'] @entity13214 :: ('MESH:C070080', 'Chemical') :: ['PKH26'] @entity1562 :: ('MESH:D017682', 'Disease') :: ['myocardium'] @entity236 :: ('MESH:D001855', 'Disease') :: ['bone marrow aspiration']
@entity583 :: (MESH:D009203,Disease) :: ['myocardial infarction']
