BACKGROUND: Previous work by our group and others has implicated a role for kinins in the ovulatory process. The purpose of the present study was to elucidate whether endogenous @entity1420 , which is an intraovarian regulator of ovulation, might be responsible for induction of the kinin system in the ovary during ovulation. The gonadotropin-primed immature @entity35 was used as the experimental model, and the role of endogenous @entity1420 was explored using the antiprogestin, @entity3927 . RESULTS: The results of the study revealed that @entity3927 treatment, as expected, significantly attenuated ovulation. Activity of the kinin-generating enzyme, @entity7199 , was elevated in the ovary in control animals prior to ovulation with peak values observed at 4 h post hCG, only to fall to low levels at 10 h, with a recovery at 20 h post hCG. @entity3927 treatment had no significant effect on ovarian kallikrein activity as compared to the control group. Total ovarian kininogen levels in control animals increased significantly at 12-14 h after hCG - coinciding with @entity1613 . Thereafter, ovarian kininogen levels fell to low levels at 20 h, only to show a rebound from 24-38 h post-hCG. @entity3927 treatment had no significant effect on the rise of total ovarian kininogen levels from 12-14 h after hCG; however, from 30-40 h post hCG, @entity3927 -treated animals had significantly higher total ovarian kininogen levels versus control animals, suggesting that endogenous @entity1420 may act to restrain elevations of kininogens in the post-ovulatory ovary. This robust elevation of ovarian kininogen levels by @entity3927 was found to be primarily due to an increase in T-kininogen, which is a potent @entity462 protease inhibitor. CONCLUSIONS: Taken as a whole, these results suggest that endogenous @entity1420 does not regulate kallikrein activity or kininogens prior to ovulation, but may provide a restraining effect on T-kininogen levels in the post-ovulatory ovary.

Activation of the kinin system in the ovary during ovulation: role of endogenous XXXX .

@entity462 :: ('MESH:D003545', 'Chemical') :: ['cysteine'] @entity7199 :: ('MESH:D007610', 'Chemical') :: ['kallikrein'] @entity1420 :: ('MESH:D011374', 'Chemical') :: ['progesterone'] @entity1613 :: ('MESH:D011230', 'Disease') :: ['initiation of ovulation'] @entity35 :: ('10116', 'Species') :: ['rat'] @entity3927 :: ('MESH:D015735', 'Chemical') :: ['RU486']
@entity1420 :: (MESH:D011374,Chemical) :: ['progesterone']
