PURPOSE: To determine the antimicrobial activity of the @entity69368 derived peptide Mel4 against Delftia, Stenotrophomonas, Elizabethkingia, Burkholderia and to investigate biocompatibility of Mel4 as an antimicrobial coating on contact lenses in animals and @entity1 . METHODS: In vitro antimicrobial activity of Mel4 was determined against the four Gram negative bacteria by investigating growth curves for 24h followed by viable counts to determine the minimum inhibitory concentration (MIC). Contact lenses were coated by covalently binding Mel4, characterized by @entity3689 analysis, and were investigated for changes in lens parameters. Safety of Mel-4 coated lenses were determined in a @entity95 model of daily contralateral wear. A prospective, randomised, double-masked, contralateral, 1week daily wear @entity1 clinical trial was used to evaluate subjective responses and ocular physiology. RESULTS: Mel4 was active against all the bacteria tested (MIC50 ranged from 31-1000 gml(-1)) and produced an antimicrobial surface on contact lenses. Mel4-coating resulted hydrophilic surface without any significant change in contact lens parameters, and showed no signs of @entity137 or ocular irritation during @entity95 wear. During @entity1 clinical trial, there were no differences between Mel4 coated and uncoated contact lenses in lens performance indicators and ocular signs such as corneal @entity941 staining. Mel4 and control uncoated lenses had no differences in ocular symptoms during lens wear. CONCLUSION: Mel4 has achieved antimicrobial activity against variety of Gram negative bacteria that are often resistant to the action of cationic peptides and have been implicated in contact lens related adverse events. Mel4-coated contact lenses were safe to wear.

Activity of a XXXX derived peptide Mel4 against Stenotrophomonas, Delftia, Elizabethkingia, Burkholderia and biocompatibility as a contact lens coating.

@entity1 :: ('9606', 'Species') :: ['humans', 'human'] @entity69368 :: ('MESH:C541912', 'Chemical') :: ['melimine'] @entity137 :: ('MESH:D064420', 'Disease') :: ['cytotoxicity'] @entity95 :: ('9986', 'Species') :: ['rabbit'] @entity941 :: ('MESH:D019793', 'Chemical') :: ['fluorescein'] @entity3689 :: ('D000596', 'Chemical') :: ['amino acid']
@entity69368 :: (MESH:C541912,Chemical) :: ['melimine']
