BACKGROUND: Systemic delivery of bone marrow-derived mesenchymal stem cells (BM-MSCs) is an attractive approach for myocardial repair. We aimed to test this strategy in a @entity35 model after @entity583 ( @entity583 ). METHODS AND RESULTS: BM-MSCs were obtained from @entity35 bone marrow, expanded in vitro to a purity of >50%, and labeled with 99mTc exametazime, fluorescent dye, LacZ marker gene, or bromodeoxyuridine. @entity35 were subjected to @entity583 by transient @entity1563 or to sham @entity583 . 99mTc-labeled cells (4x10(6)) were transfused into the left ventricular cavity of @entity583 @entity35 either at 2 or 10 to 14 days after @entity583 and were compared with sham- @entity583 @entity35 or @entity583 @entity35 treated with intravenous infusion. Gamma camera imaging and isolated organ counting 4 hours after intravenous infusion revealed uptake of the 99mTc-labeled cells mainly in the lungs, with significantly smaller amounts in the liver, heart, and spleen. Delivery by left ventricular cavity infusion resulted in drastically lower lung uptake, better uptake in the heart, and specifically higher uptake in infarcted compared with sham- @entity583 hearts. Histological examination at 1 week after infusion identified labeled cells either in the @entity1188 zone but not in remote viable @entity1562 or sham- @entity583 hearts. Labeled cells were also identified in the lung, liver, spleen, and bone marrow. CONCLUSIONS: Systemic intravenous delivery of BM-MSCs to @entity35 after @entity583 , although feasible, is limited by entrapment of the donor cells in the lungs. Direct left ventricular cavity infusion enhances migration and colonization of the cells preferentially to the @entity63 @entity1562 .

Systemic delivery of bone marrow-derived mesenchymal stem cells to the infarcted XXXX : feasibility, cell migration, and body distribution.

@entity63 :: ('MESH:D007511', 'Disease') :: ['ischemic'] @entity1563 :: ('MESH:D054059', 'Disease') :: ['coronary artery occlusion'] @entity1188 :: ('MESH:D007238', 'Disease') :: ['infarcted or border'] @entity1562 :: ('MESH:D017682', 'Disease') :: ['myocardium'] @entity35 :: ('10116', 'Species') :: ['rat', 'Rats', 'rats'] @entity583 :: ('MESH:D009203', 'Disease') :: ['myocardial infarction', 'MI']
@entity1562 :: (MESH:D017682,Disease) :: ['myocardium']
