OBJECTIVE: To investigate the effects of @entity24770 on chondrocytes cultured with or without @entity20655 . DESIGN: @entity235 articular chondrocytes were cultured in three-dimensional (3-D) agarose constructs. Cells were distributed into each of the two groups, those without @entity20655 and those with @entity20655 added to the liquid media. Each group was subdivided into three groups, based on the presence of @entity24770 . @entity20655 and TIMPs were added to liquid media bathing the 3-D constructs beginning on day 3. The liquid media and the 3-D constructs were collected on days 9, 15, and 24, and analyzed histologically, biochemically, and immunohistochemically. RESULTS: Addition of @entity11800 or -2 resulted in decreases in @entity22308 concentrations of 37 and 41%, and @entity56358 immunoreactivity of 32 and 36%, respectively, compared with the @entity20655 group, on day 9. Chondrocytes in groups without @entity20655 maintained viability and produced abundant extracellular matrix (ECM). Chondrocytes in @entity20655 groups appeared less viable and produced less ECM compared with those without @entity20655 . Glycosaminoglycan (GAG) concentrations in 3-D constructs (GAG/weight) were significantly (P<0.001) higher in groups without @entity20655 than in those with @entity20655 , on days 15 and 24. CONCLUSIONS: The addition of TIMP was not detrimental to chondrocytes, as used in this study. Despite evidence of decreased MMP levels, TIMPs did not prevent @entity20655 -associated changes in cellular or ECM characteristics. Further study is necessary before clinically relevant conclusions can be drawn regarding the use of TIMPs in the treatment of @entity571 .

The effects of XXXX on @entity235 chondrocytes cultured in three-dimensional agarose culture system.

@entity22308 :: ('403445(Tax:9615)', 'Gene') :: ['matrix metalloproteinase (MMP)-3'] @entity56358 :: ('489428(Tax:9615)', 'Gene') :: ['MMP-1'] @entity571 :: ('MESH:D010003', 'Disease') :: ['osteoarthritis'] @entity235 :: ('9615', 'Species') :: ['Canine'] @entity20655 :: ('403974(Tax:9615)', 'Gene') :: ['IL-1 beta', 'interleukin (IL)-1 beta'] @entity11800 :: ('403816(Tax:9615)', 'Gene') :: ['TIMP-1'] @entity24770 :: ('403816;403633', 'Gene') :: ['tissue inhibitor of metalloproteinase (TIMP)-1 and -2', 'TIMP-1 or -2']
@entity24770 :: (403816;403633,Gene) :: ['TIMP-1 and -2']
