Novel targeted proapoptotic anticancer drug delivery systems were developed and evaluated. @entity968 ( @entity970 ) conjugates were used as carriers. @entity9800 ( @entity1191 ) was used as an anticancer agent-apoptosis inductor. Two types of molecular targets were investigated: (1) an extracellular membrane receptor specific to @entity43 and (2) intracellular controlling mechanisms of apoptosis. Synthetic peptides similar to luteinizing hormone-releasing hormone (LHRH) and @entity588 homology 3 ( @entity2424 ) peptide were used as a targeting moiety and a suppressor of cellular antiapoptotic defense, respectively. Three different conjugates ( @entity1191 - @entity970 , @entity1191 - @entity970 - @entity2424 and @entity1191 - @entity970 - @entity1176 ) were synthesized and examined in A2780 @entity1 @entity43 cells. @entity137 , expression of genes encoding @entity588 , @entity1836 , @entity17205 , @entity3437 proteins and @entity9189 , the activity of @entity9189 and apoptosis induction were studied. Taken together the results indicate much higher @entity137 and apoptosis-inducing activity of @entity970 - @entity1191 conjugates when compared to free @entity1191 . Moreover, the effects of targeted @entity1191 - @entity970 - @entity2424 and @entity1191 - @entity970 -LHRH conjugates were more pronounced than the non-targeted @entity970 - @entity1191 conjugate. The results confirmed the feasibility of this new two-tier molecular targeting strategy for enhancing the efficacy of @entity5 chemotherapy.

Molecular targeting of drug delivery systems to @entity43 by XXXX and LHRH peptides.

@entity43 :: ('MESH:D010051', 'Disease') :: ['ovarian cancer'] @entity1 :: ('9606', 'Species') :: ['human'] @entity3437 :: ('317', 'Gene') :: ['APAF-1'] @entity137 :: ('MESH:D064420', 'Disease') :: ['Cytotoxicity', 'cytotoxicity'] @entity17205 :: ('56616', 'Gene') :: ['SMAC'] @entity588 :: ('596', 'Gene') :: ['BCL-2'] @entity9189 :: ('836;842', 'Gene') :: ['caspases 3 and 9'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity1836 :: ('598', 'Gene') :: ['BCL-XL'] @entity1191 :: ('CHEBI:27656', 'Chemical') :: ['CPT'] @entity9800 :: ('MESH:D002166', 'Chemical') :: ['Camptothecin'] @entity1176 :: ('MESH:D007987', 'Chemical') :: ['LHRH'] @entity970 :: ('CHEBI:46793', 'Chemical') :: ['PEG'] @entity968 :: ('MESH:D011092', 'Chemical') :: ['Poly(ethyleneglycol)'] @entity2424 :: ('MESH:C006008', 'Chemical') :: ['BH3']
@entity2424 :: (MESH:C006008,Chemical) :: ['BH3']
