OBJECTIVE: To generate phage-displayed anti-idiotypic antibody single chain variable fragments (anti-Id ScFv) to MG7 monoclonal antibody (McAb) directed against @entity356 so as to lay a foundation for developing anti-Id ScFv vaccine of the @entity5 . METHODS: Balb/c @entity19 were immunized i.p. with MG7 McAb conjugated with keyhole limpet hemocyanin (KLH), and mRNA was isolated from the spleens of the immunized @entity19 . Heavy and light chain (VH and VL) genes of antibody were amplified separately and assembled into ScFv genes with a linker DNA by PCR. The ScFv genes were ligated into the phagemid vector pCANTAB5E and the ligated sample was transformed into competent @entity526 TG1. The transformants were infected with M13K07 helper phage to yield recombinant phages displaying ScFv on the tips of M13 phage. After 4 rounds of panning with MG7, the MG7-positive clones were selected by ELISA from the enriched phages. The types of the anti-Id ScFv displayed on the selected phage clones were preliminarily identified by competition ELISA. RESULTS: The VH, VL and ScFv DNAs were about 340 bp, 320 bp and 750 bp respectively. Twenty-four MG7-positive clones were selected from 60 enriched phage clones, among which 5 displayed beta or gamma type anti-Id ScFv. CONCLUSION: The anti-Id ScFv to MG7 McAb can be successfully selected by recombinant phage antibody technique, which paves a way for the study of prevention and cure of @entity356 by using anti-Id ScFv.

Production of phage-displayed anti-idiotypic antibody single chain variable fragments to MG7 monoclonal antibody directed against XXXX .

@entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity19 :: ('10090', 'Species') :: ['mice'] @entity356 :: ('MESH:D013274', 'Disease') :: ['gastric carcinoma'] @entity526 :: ('562', 'Species') :: ['E. coli']
@entity356 :: (MESH:D013274,Disease) :: ['gastric carcinoma']
