OBJECTIVE: Autologous bone marrow transplantation in @entity5 @entity1 is often preceded by multiple cycles of chemotherapy. In this study, we assessed in a @entity19 model whether stem cells were affected by prior chemotherapy. METHODS: Donor @entity19 were treated with three consecutive injections of 150 mg/kg @entity1487 ( @entity1487 ). Peripheral blood counts were allowed to recover before the subsequent dose of @entity1487 was given. @entity19 recovered from three doses of @entity1487 and showed normal steady-state hematopoiesis. Bone marrow cells from these @entity19 were mixed with congenic competitor cells and transplanted into lethally irradiated recipients. RESULTS: Although in vivo homing of cells from these @entity19 was not impaired, donor leukocyte contribution steadily decreased posttransplantation. In contrast to in vivo homing, both in vitro migration toward @entity19149 and the average @entity9843 ( @entity9843 ) expression were lower in @entity1487 -treated cells. Moderate reductions in @entity6232 and @entity4459 expression were observed on stem cells of @entity1487 -treated @entity19 . @entity64756 , @entity3844 , @entity27063 , and @entity63963 were normally expressed and could thus not explain the reduced engraftment of these cells. CONCLUSION: We therefore conclude that @entity1487 either directly damages stem cells or that the replicative stress induced by @entity1487 causes a decline in stem cell reconstitution potential resulting in lower chimerism levels posttransplantation, that declines in time.

Chemotherapy prior to autologous bone marrow transplantation impairs long-term engraftment in XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity64756 :: ('20737(Tax:10090)', 'Gene') :: ['CD43'] @entity5 :: ('MESH:D009369', 'Disease') :: ['cancer'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'Mice'] @entity9843 :: ('12767(Tax:10090)', 'Gene') :: ['CXCR4', 'CXC chemokine receptor-4'] @entity19149 :: ('20315(Tax:10090)', 'Gene') :: ['stromal-derived factor (SDF)-1'] @entity4459 :: ('16408(Tax:10090)', 'Gene') :: ['CD11a'] @entity27063 :: ('16401(Tax:10090)', 'Gene') :: ['CD49d'] @entity6232 :: ('20343(Tax:10090)', 'Gene') :: ['L-selectin'] @entity63963 :: ('16402(Tax:10090)', 'Gene') :: ['CD49e'] @entity3844 :: ('12505(Tax:10090)', 'Gene') :: ['CD44'] @entity1487 :: ('MESH:D005472', 'Chemical') :: ['5-FU', '5-fluorouracil']
@entity19 :: (10090,Species) :: ['mice']
