Pathogenesis-related proteins of group 1 (PR-1) are strongly induced in plants by pathogen attack, exposure of the plants to @entity117 ( @entity1281 , SA), and by developmental cues. Functional analysis of the PR-1a promoter identified a region of 139 bp (from -691 to -553) mediating expression of the GUS reporter gene in response to @entity1281 . Inspection of this region revealed two TGACG elements reminiscent of activation sequence-1 (as-1). Recently, as-1 has been reported to be responsive to SA in the context of the @entity3260 35S RNA promoter. To address the question of whether the as-1-like sequence may be of functional significance for the expression of the PR-1a gene, gel shift assays were performed with TGA1a, a protein been shown to interact with as-1 in vitro. TGA1a was found to bind to the PR-1a as-1-like sequence with similar specificity and affinity as to as-1. Furthermore, mutations were introduced in the as-1-like sequence in the context of the inducible 906 bp PR-1a promoter which are impaired in binding TGA1a in vitro. Significantly reduced levels of GUS reporter gene activity were obtained with the mutant promoter regions as compared to the wild-type PR-1a promoter in response to all stimuli in transgenic @entity286 plants. Yet, mutation of the as-1-like sequence did not abolish induction of reporter gene expression. Taken together, these results suggest that the level of expression of the @entity286 PR-1a gene is controlled by an as-1-like sequence motif in the PR-1a upstream region, possibly interacting with a factor related to TGA1a.

An as-1-like motif controls the level of expression of the gene for the pathogenesis-related protein 1a from XXXX .

@entity286 :: ('4097', 'Species') :: ['tobacco'] @entity3260 :: ('10641', 'Species') :: ['CaMV'] @entity117 :: ('MESH:D001241', 'Chemical') :: ['(acetyl)salicylic acid'] @entity1281 :: ('CHEBI:15365', 'Chemical') :: ['ASA']
@entity286 :: (4097,Species) :: ['tobacco']
