As the Ebola outbreak in West Africa continues and cases appear in the United States and other countries, the need for long-lasting vaccines to preserve global health is imminent. Here, we evaluate the long-term efficacy of a respiratory and sublingual (SL) adenovirus-based vaccine in non- @entity1 primates in two phases. In the first, a single respiratory dose of 1.4 * 10(9) infectious virus particles (ivp)/kg of Ad-CAGoptZGP induced strong Ebola glycoprotein (GP) specific @entity405 (+) and @entity404 (+) T cell responses and Ebola GP-specific antibodies in systemic and mucosal compartments and was partially (67%) protective from challenge 62 days after immunization. The same dose given by the SL route induced Ebola GP-specific @entity405 (+) T cell responses similar to that of intramuscular (IM) injection, however, the Ebola GP-specific antibody response was low. All primates succumbed to @entity281 . Three primates were then given the vaccine in a formulation that improved the immune response to Ebola in rodents. Three primates were immunized with 2.0 * 10(10) ivp/kg of vaccine by the SL route. Diverse populations of polyfunctional Ebola GP-specific @entity404 (+) and @entity405 (+) T cells and significant anti-Ebola GP antibodies were present in samples collected 150 days after respiratory immunization. The formulated vaccine was fully protective against challenge 21 weeks after immunization. While diverse populations of Ebola GP-specific @entity404 (+) T cells were produced after SL immunization, antibodies were not neutralizing and the vaccine was unprotective. To our knowledge, this is the first time that durable protection from a single dose respiratory adenovirus-based Ebola vaccine has been demonstrated in primates.

A Single Dose Respiratory Recombinant Adenovirus-Based Vaccine Provides Long-Term Protection for Non- XXXX Primates from Lethal Ebola Infection.

@entity1 :: ('9606', 'Species') :: ['human'] @entity404 :: ('920', 'Gene') :: ['CD4'] @entity405 :: ('925', 'Gene') :: ['CD8'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection']
@entity1 :: (9606,Species) :: ['Human']
