BACKGROUND: In a previous proteomics study using pooled cerebrospinal fluid (CSF) samples, we proposed apolipoprotein AI, apolipoprotein AIV, @entity4732 , plasminogen, semaphorin 7A, and ala-b-his-dipeptidase as candidate biomarkers associated with the conversion to @entity15 ( @entity15 ) in @entity1 with @entity922 ( @entity922 ). Here, we aimed to validate these results in individual CSF samples using alternative techniques. METHODS: In a first replication study, levels of apolipoproteins AI and AIV, @entity4732 , and plasminogen were measured by ELISA in CSF and serum of 56 @entity922 @entity1 (29 @entity1 who converted to @entity15 (MS converters) and 27 @entity1 who remained with @entity922 during follow-up (MS non-converters)) and 26 controls with other @entity16 . Semaphorin 7A and ala-b-his-dipeptidase levels were determined by selected reaction monitoring (SRM) in CSF of 36 @entity1 (18 MS converters, 18 non-converters) and 20 controls. In a second replication study, apolipoprotein AI levels were measured by ELISA in CSF of 74 @entity922 @entity1 (47 MS converters, 27 non-converters) and 50 individual controls, and levels of semaphorin 7A and ala-beta-his-dipeptidase were determined by SRM in 49 @entity1 (24 MS converters, 25 non-converters) and 22 controls. RESULTS: CSF levels of apolipoprotein AI were increased (P = 0.043) and levels of semaphorin 7A and ala-b-his-dipeptidase decreased (P = 4.4 * 10(-10) and P = 0.033 respectively) in MS converters compared to non-converters. No significant differences were found in serum levels for apolipoproteins AI and AIV, @entity4732 , and plasminogen. Findings with semaphorin 7A and ala-b-his-dipeptidase were also validated in the second replication study, and CSF levels for these two proteins were again decreased in MS converters versus non-converters (P = 1.2 * 10(-4) for semaphorin 7A; P = 3.7 * 10(-8) for ala-b-his-dipeptidase). Conversely, apolipoprotein AI findings were not replicated and CSF levels for this protein did not significantly differ between groups. Furthermore, CSF semaphorin 7A levels were negatively associated with the number of T2 lesions at baseline and one-year follow-up. CONCLUSIONS: These results validate previous findings for semaphorin 7A and ala-b-his-dipeptidase, and suggest that these proteins play a role as CSF biomarkers associated with the conversion to @entity15 in @entity922 @entity1 .

Validation of semaphorin 7A and ala-b-his-dipeptidase as biomarkers associated with the conversion from clinically isolated syndrome to XXXX .

@entity1 :: ('9606', 'Species') :: ['patients'] @entity16 :: ('MESH:D009422', 'Disease') :: ['neurological disorders'] @entity15 :: ('MESH:D009103', 'Disease') :: ['clinically definite multiple sclerosis', 'CDMS'] @entity4732 :: ('7448', 'Gene') :: ['vitronectin'] @entity922 :: ('MESH:D013568', 'Disease') :: ['clinically isolated syndromes', 'CIS']
@entity15 :: (MESH:D009103,Disease) :: ['multiple sclerosis']
