UNASSIGNED: Sepsis is a major cause of @entity204 for hospitalized @entity1 and is characterized by massive overreaction of immune responses to invading pathogens which is mediated by cytokines. For decades, there has been no effective treatment for @entity295 . @entity68910 ( @entity68910 ), is an immunomodulatory receptor expressed primarily on hematopoietic cells which is involved in various aspects of inflammatory responses and is a potential target for treatment of @entity295 . The aim of the present study was to develop a human anti- @entity68910 @entity1334 fragment, which was named @entity31975 -Fab03 and investigate its immune activity in @entity1 macrophages. We began by constructing the @entity31975 -Fab03 prokaryotic expression vector from human antibody library and phage display. Then, we utilized a multitude of assays, including @entity214 -PAGE, Western blotting, ELISA, affinity, and kinetics assay to evaluate the binding affinity and specificity of @entity31975 -Fab03. Results demonstrated that @entity31975 -Fab03 specifically bind to @entity68910 antigen with high affinity, and pretreatment with @entity31975 -Fab03 could attenuate lipopolysaccharide (LPS)-induced @entity310 , @entity650 , @entity1356 , @entity687 , and @entity2391 production in @entity1 PBMC-derived macrophages, but slightly increased @entity688 production in an early time point. We also observed similar results in @entity1 @entity1357 -differentiated macrophages. Collectively, we prepared the @entity31975 -Fab03 with efficient activity for blocking LPS-induced pro-inflammatory cytokines production in @entity1 macrophages. These results indicated that ligation of @entity68910 with @entity31975 -Fab03 might be a novel anti-inflammatory therapeutic strategy for @entity295 .

The @entity1334 Fragment of a Human Anti- @entity68910 Monoclonal Antibody Suppresses LPS-Induced Inflammatory Responses in XXXX Macrophages.

@entity1 :: ('9606', 'Species') :: ['human', 'patients'] @entity650 :: ('3569', 'Gene') :: ['IL-6'] @entity1357 :: ('2736', 'Gene') :: ['THP-1'] @entity295 :: ('MESH:D018805', 'Disease') :: ['sepsis'] @entity1356 :: ('3553', 'Gene') :: ['IL-1b'] @entity68910 :: ('27180', 'Gene') :: ['Siglec-9', 'Sialic acid-binding, Ig-like lectin-9'] @entity310 :: ('7124', 'Gene') :: ['TNF-a'] @entity1334 :: ('2187', 'Gene') :: ['Fab'] @entity31975 :: ('6203', 'Gene') :: ['hS9'] @entity688 :: ('3586', 'Gene') :: ['IL-10'] @entity2391 :: ('3456', 'Gene') :: ['IFN-b'] @entity687 :: ('3576', 'Gene') :: ['IL-8'] @entity204 :: ('MESH:D003643', 'Disease') :: ['death'] @entity214 :: ('CHEBI:8984', 'Chemical') :: ['SDS']
@entity1 :: (9606,Species) :: ['Human']
