UNASSIGNED: Syndecans are heparan sulfate proteoglycans characterized as transmembrane receptors that act cooperatively with the cell surface and extracellular matrix proteins. @entity49561 knockdown was performed in order to address its role in endothelial cells (EC) behavior. Normal EC and shRNA- @entity49561 -EC cells were studied comparatively using complementary confocal, super-resolution and non-linear microscopic techniques. Confocal and super-resolution microscopy revealed that @entity49561 knockdown alters the level and arrangement of essential proteins for focal adhesion, evidenced by the decoupling of @entity10274 from F-actin filaments. Furthermore, @entity49561 knockdown alters the actin network leading to filopodial protrusions connected by @entity3545 -rich junction. shRNA- @entity49561 -EC showed reduced adhesion and increased migration. Also, @entity49561 silencing alters cell cycle as well as cell proliferation. Moreover, the ability of EC to form tube-like structures in matrigel is reduced when @entity49561 is silenced. Together, the results suggest a mechanism in which @entity13224 acts as a central mediator that bridges @entity8946 , integrin and intracellular components (actin and @entity10274 ) and once silenced, the cytoskeleton protein network is disrupted. Ultimately, the results highlight @entity49561 relevance for balanced cell behavior.

Coupling of XXXX to F-actin demands @entity13224 proteoglycan.

@entity49561 :: ('54212', 'Gene') :: ['Syn4'] @entity3545 :: ('1003', 'Gene') :: ['VE-cadherin'] @entity10274 :: ('7414', 'Gene') :: ['vinculin'] @entity13224 :: ('6385', 'Gene') :: ['Syndecan-4'] @entity8946 :: ('2335', 'Gene') :: ['fibronectin']
@entity10274 :: (7414,Gene) :: ['vinculin']
