OBJECTIVES: To construct a @entity157 directed gene delivery system which could transfer preS2 antisense RNA to @entity96 cells specifically, and to explore a new therapeutic strategy for @entity157 by blocking @entity1956 virus (HBV) with antisense RNA targeting @entity157 . METHODS: GE7 and HA20 were synthesized and mixed with pEBAF-as-preS2, a hepatocarcinoma specific HBV antisense expression vector, to construct a novel HBV antisense RNA delivery system named AFP-enhancing 4-element complex. @entity19 bearing hepatocelluar @entity189 cells HepG2.2.15 were injected with AFP-enhancing 4-element complex via a tail vein. Total RNA from tissues was extracted, and reversal transcription-ploymerase chain reaction (RT-PCR) was used to detect the expression of preS2. Different doses of AFP-enhancing 4-element complex was injected into @entity19 at different time points, and @entity5 diameter was measured. RESULTS: AFP-enhancing 4-element complex was constructed successfully. RT-PCR showed preS2 antisense RNA delivered by AFP-enhancing 4-element complex only expressed in @entity96 HepG @entity1 cells of the @entity19 . After the treatment of AFP-enhancing 4-element complex with dose of 0.2 micro g per @entity19 (once a week for 4 weeks), the mean @entity5 diameter of @entity19 was significantly shorter than that of the control groups (0.995 +/- 0.35 cm vs 2.125 +/- 0.25 cm, P < 0.01). CONCLUSIONS: An HBV antisense RNA gene delivery system targeting @entity157 , AFP-enhancing 4-element complex, was constructed successfully. PreS2 antisense RNA expressed specifically in hepatocelluar @entity189 cells significantly inhibits @entity5 growth of @entity19 @entity35030 HepG2.2.15 and may have therapeutic potential in @entity97 related hepatocarcinoma.

Effect of preS2 antisense RNA on XXXX with a novel delivery system.

@entity1 :: ('9606', 'Species') :: ['2.2.15'] @entity97 :: ('10407', 'Species') :: ['HBV'] @entity1956 :: ('MESH:D006509', 'Disease') :: ['hepatitis B'] @entity96 :: ('MESH:D008113', 'Disease') :: ['liver tumor', 'liver cancer'] @entity19 :: ('10090', 'Species') :: ['mice', 'mouse', 'Nude mice', 'nude mice'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor'] @entity35030 :: ('MESH:C565129', 'Disease') :: ['bearing hepatocarcinoma'] @entity189 :: ('MESH:D002277', 'Disease') :: ['carcinoma'] @entity157 :: ('MESH:D006528', 'Disease') :: ['hepatoma', 'hepatocellular carcinoma']
@entity157 :: (MESH:D006528,Disease) :: ['hepatocellular carcinoma']
