UNASSIGNED: Macrophages are involved in @entity5 progression. M1 macrophages have an antitumor effect, whereas M2 phenotypes are associated with @entity5 growth. The progression of @entity11 involves the participation of an inflammatory microenvironment. @entity855 ( @entity855 ) can act as pro-inflammatory signal, whereas @entity859 has opposite properties. The biological effects of extracellular @entity2036 / @entity2642 mediated by purinergic receptors are controlled by ectonucleotidases. In the present work, we evaluated whether @entity11 -conditioned medium (GL-CM) modulates macrophage differentiation and the participation of @entity855 and @entity859 in the release of pro-and anti-inflammatory cytokines by these cells. The results show that macrophages exposed to GL-CM were modulated to an M2-like phenotype. HPLC analysis of GL-CM demonstrated the presence of significant amounts of @entity855 and its metabolites. Macrophages exposed to GL-CM presented decreased @entity855 and @entity860 hydrolysis and increased @entity688 and @entity2842 secretion, effects that were diminished by P1 or P2 antagonists. GL-CM did not alter the release of @entity650 by macrophages, although treatment with @entity855 promoted an increase in the release of @entity650 , which was prevented by a @entity9896 antagonist. In summary, we found that A2A and @entity9896 activation is necessary for @entity688 , @entity2842 and @entity650 release by macrophages exposed to GL-CM, which, in turn, modulates the macrophages to M2-phenotype. The present study establishes a relationship between M2-like polarization, cytokine release and purinergic receptor activation in macrophages exposed to GL-CM. Therefore, the data presented herein contributes to advancing in the field of @entity5 and point specific purinergic receptors as targets for modulation of the phenotype of @entity11 -associated macrophages. This article is protected by copyright. All rights reserved.

Involvement of purinergic system in the release of cytokines by macrophages exposed to XXXX -conditioned medium.

@entity650 :: ('3569', 'Gene') :: ['IL-6'] @entity2642 :: ('MESH:D009705', 'Chemical') :: ['nucleosides'] @entity859 :: ('MESH:D000241', 'Chemical') :: ['adenosine'] @entity2036 :: ('MESH:D009711', 'Chemical') :: ['nucleotides'] @entity5 :: ('MESH:D009369', 'Disease') :: ['tumor', 'cancer', 'cancer-related inflammation'] @entity11 :: ('MESH:D005910', 'Disease') :: ['glioma', 'gliomas'] @entity2842 :: ('6347', 'Gene') :: ['MCP-1'] @entity9896 :: ('5027', 'Gene') :: ['P2X7'] @entity860 :: ('MESH:D000249', 'Chemical') :: ['AMP'] @entity688 :: ('3586', 'Gene') :: ['IL-10'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['Adenosine triphosphate', 'ATP']
@entity11 :: (MESH:D005910,Disease) :: ['glioma']
