BACKGROUND: Improved organ preservation is essential for the success of small bowel transplantation. Small bowel is usually preserved in UW (University of Wisconsin) solution which does not contain @entity1160 ( @entity7647 ), the principal fuel for the enterocyte. We hypothesized that @entity7647 -supplemented UW would improve mucosal function and structure of cold preserved small intestine. MATERIALS AND METHODS: Jejunum (40 cm) was harvested from Lewis @entity35 and preserved for 18 hr at 4 degrees C in saline; UW solution only; UW with 1, 2, or 4% @entity7647 ; and UW containing 1, 2, or 4% isonitrogenous balanced nonessential @entity1522 (NEAA). @entity3209 glucose transport, mucosal protein, mucosal maltase and alkaline phosphatase, jejunal villous height, and histologic damage were measured. RESULTS: UW with 2% @entity7647 significantly increased @entity413 transport and mucosal protein when compared to the 2% NEAA and UW-only groups. Two percent @entity7647 significantly decreased histologic damage of jejunum following cold preservation. Increasing @entity7647 to 4% did not significantly increase its efficacy when compared to the UW with 2% @entity7647 group. There were no significant differences in the activities of mucosal maltase and alkaline phosphatase among the various treatment groups. CONCLUSIONS: The addition of @entity7647 , optimally provided at a concentration of 2%, to UW solution may protect the preserved small bowel segments from @entity63 and improve mucosal function.

XXXX protects function and improves preservation of small bowel segments.

@entity1522 :: ('CHEBI:33709', 'Chemical') :: ['amino acids'] @entity1160 :: ('CHEBI:28300', 'Chemical') :: ['glutamine'] @entity63 :: ('MESH:D007511', 'Disease') :: ['cold ischemic injury'] @entity7647 :: ('CHEBI:32668', 'Chemical') :: ['Gln'] @entity3209 :: ('CHEBI:36927', 'Chemical') :: ['14C'] @entity35 :: ('10116', 'Species') :: ['rats'] @entity413 :: ('MESH:D005947', 'Chemical') :: ['glucose']
@entity1160 :: (CHEBI:28300,Chemical) :: ['Glutamine']
