UNASSIGNED: @entity2316 ( @entity2316 ) is the main cause of morbidity and mortality in @entity6 , characterized by mesangial matrix deposition and podocytopathy including podocyte loss. The risk factors and mechanisms involved in the pathogenesis of @entity2316 are still not completely defined. In the current study we aim to understand the cellular mechanisms through which activation of B2 kinin receptors contribute to the initiation and progression of @entity2316 . Stimulation of cultured @entity35 podocytes with @entity1812 (BK) resulted in a significant increase in reactive @entity26 species generation (ROS) and this was associated with a significant increase in @entity12613 and @entity8563 protein and mRNA levels. BK stimulation also resulted in a signicant increase in the phosphorylation of @entity2508 and Akt and this effect was inhibited in the presence of @entity12613 and @entity8563 siRNA. Furthermore podocytes stimulated with BK resulted in a significant increase in the protein and mRNA levels of @entity2528 ( @entity2528 ) and at the same time a significant decrease in the protein and mRNA levels of nephrin. siRNA targeted against @entity12613 and @entity8563 significantly inhibited the BK-induced increase in @entity2528 . Nephrin expression was increased in response to BK in the presence of @entity12613 and @entity8563 siRNA, thus implicating a role for NOXs in modulating the BK response in podocytes. Moreover, nephrin expression in response to BK was also significantly increased in the presence of siRNA targeted against @entity2528 . These findings provide novel aspects of BK signal transduction pathways in pathogenesis of @entity2316 and identify novel targets for interventional strategies.

Mechanisms of XXXX -Induced Expression of @entity2528 and Nephrin in Podocytes.

@entity2508 :: ('50689;116590', 'Gene') :: ['ERK1/2'] @entity6 :: ('MESH:D003920', 'Disease') :: ['diabetes'] @entity8563 :: ('85431(Tax:10116)', 'Gene') :: ['Nox4', 'NOX4'] @entity2316 :: ('MESH:D003928', 'Disease') :: ['DN', 'Diabetic Nephropathy'] @entity26 :: ('MESH:D010100', 'Chemical') :: ['oxygen'] @entity12613 :: ('114243(Tax:10116)', 'Gene') :: ['NOX1'] @entity2528 :: ('64032(Tax:10116)', 'Gene') :: ['CTGF', 'connective tissue growth factor'] @entity1812 :: ('MESH:D001920', 'Chemical') :: ['bradykinin'] @entity35 :: ('10116', 'Species') :: ['rat']
@entity1812 :: (MESH:D001920,Chemical) :: ['Bradykinin']
