UNASSIGNED: Retinal M  ller glial cells have already been implicated in @entity1020 ( @entity1020 ). @entity1020 is characterized by accumulation of toxic amyloid-b peptide (Ab); the question we raise is as follows: is @entity9896 , known to play an important role in several @entity179 , involved in Ab @entity137 on M  ller cells? Retinal M  ller glial cells were incubated with Ab for 48  h. Cell viability was assessed using the alamarBlue assay and @entity137 using the @entity880 dehydrogenase (LDH) release assay. @entity9896 expression was highlighted by immunolabeling observed on confocal microscopy and its activation was evaluated by @entity17005 assay. @entity5956 was used to evaluate chromatin condensation, and caspases 8 and 3 activation was assessed using AMC assays. Lipid formulation rich in @entity7784 ( @entity8393 ) and @entity4600 ( @entity4601 ) used in Age-Related @entity2194 Study 2 was incubated on cells for 15  min prior to Ab incubation. For the first time, we showed that Ab induced caspase-independent apoptosis through @entity9896 activation on our retinal model. @entity4601 and @entity8393 are @entity1474 recommended in food supplement to prevent @entity1020 . We therefore modulated Ab @entity137 using a lipid formulation rich in @entity4601 and @entity8393 to have a better understanding of the results observed in clinical studies. We showed that fish oil rich in @entity8393 and @entity4601 , in combination with a potent @entity9896 antagonist, represents an efficient modulator of Ab @entity137 and that @entity9896 could be an interesting therapeutic target to prevent @entity1020 . Graphical Abstract    .

Amyloid b Peptide Induces Apoptosis Through @entity9896 Cell Death Receptor in Retinal Cells: Modulation by Marine Omega-3 Fatty Acid @entity4601 and XXXX .

@entity7784 :: ('MESH:D015118', 'Chemical') :: ['eicosapentaenoic acid'] @entity8393 :: ('CHEBI:28364', 'Chemical') :: ['EPA'] @entity137 :: ('MESH:D064420', 'Disease') :: ['toxicity', 'cytotoxicity'] @entity5956 :: ('MESH:C017807', 'Chemical') :: ['Hoechst 33342'] @entity2194 :: ('MESH:D005128', 'Disease') :: ['Eye Disease'] @entity4600 :: ('CHEBI:28125', 'Chemical') :: ['docosahexaenoic acid'] @entity1474 :: ('MESH:D005231', 'Chemical') :: ['polyunsaturated fatty acids'] @entity4601 :: ('CHEBI:16016', 'Chemical') :: ['DHA'] @entity179 :: ('MESH:D019636', 'Disease') :: ['degenerative diseases'] @entity880 :: ('MESH:D019344', 'Chemical') :: ['lactate'] @entity9896 :: ('5027', 'Gene') :: ['P2X7', 'P2X7 receptor'] @entity17005 :: ('MESH:C089813', 'Chemical') :: ['YO-PRO-1'] @entity1020 :: ('MESH:D008268', 'Disease') :: ['AMD', 'age-related macular degeneration']
@entity8393 :: (CHEBI:28364,Chemical) :: ['EPA']
