The moss @entity17573 , which is a land plant with efficient homologous recombination, encodes two @entity11023 proteins (PpaRad51.1 and PpaRad51.2). The PpaRad51.1 and PpaRad51.2 proteins, which share 94 % identity between them, interact with themselves and with each other. Both proteins bind ssDNA and dsDNA in a @entity1025 and pH-dependent manner, with a stoichiometry of one PpaRad51.1 monomer per 3(+/-1) nt or bp and one PpaRad51.2 monomer per 1(+/-0.5) nt or bp, respectively. At neutral pH, a 1.6-fold excess of both proteins is required for ssDNA and dsDNA binding. PpaRad51.1 and PpaRad51.2 show ssDNA-dependent ATPase activity and efficiently promote strand annealing in a @entity377 -independent but in a @entity1025 -dependent manner. Both proteins promote joint-molecule formation, DNA strand invasion and are able to catalyse strand exchange in the presence of @entity1025 and @entity855 . No further increase in the activities is observed when both proteins are present in the same reaction. None of the PpaRad51 gene products complement the DNA repair and recombination phenotype of @entity375 rad51delta mutants. However, PpaRad51.1 confers a dominant-negative DNA repair phenotype, and both PpaRad51 proteins reduce the levels of double-strand break-induced recombination when overexpressed in @entity375 wt cells. These results suggest that both PpaRad51 proteins are bona fide @entity11023 proteins that may contribute, in a different manner, to homologous recombination, and that they might replace ScRad51 in a hypothetical @entity375 protein complex inactivating different functions required for recombinational repair.

Characterization of two highly similar XXXX homologs of @entity17573 .

@entity1025 :: ('CHEBI:18420', 'Chemical') :: ['Mg(2+)'] @entity11023 :: ('856831(Tax:4932)', 'Gene') :: ['Rad51'] @entity377 :: ('CHEBI:36976', 'Chemical') :: ['nucleotide'] @entity17573 :: ('3218', 'Species') :: ['Physcomitrella patens'] @entity375 :: ('4932', 'Species') :: ['yeast', 'Saccharomyces cerevisiae', 'S. cerevisiae'] @entity855 :: ('MESH:D000255', 'Chemical') :: ['ATP']
@entity11023 :: (856831(Tax:4932),Gene) :: ['Rad51']
