@entity1068 , @entity1324 , diet and starvation, known modulators of the hepatic cytochrome P450 (CYP)-dependent microsomal monooxygenase system, were assessed for their effects on cytochrome P450 isozyme content and monooxygenase activities in the male @entity35 kidney. In acute experiments, @entity35 were either treated with @entity1324 , fasted or given a combination of the two treatments. @entity1324 treatment alone induced @entity11920 -dependent @entity11921 hydroxylase activity in kidney microsomes by 8-fold. This was accompanied by a 6-fold increase in @entity11920 apoprotein as determined by Western blot analysis. There was, however, no significant increase in steady-state levels of @entity11920 mRNA as measured by Northern blot analysis. Starvation also induced @entity11920 apoprotein in the kidney and, as has been reported previously in the liver, had a synergistic inductive effect with @entity1324 . CYP2B and @entity12531 apoproteins were also induced by @entity1324 , starvation and starvation/ @entity1324 combinations in the kidney. Immunohistochemical analysis revealed localization of @entity11920 and CYP2B principally in the cortex associated with tubular cells. This distribution was maintained upon starvation/ @entity1324 treatment. Two induction experiments were performed in which the @entity1068 was administered as part of a system of @entity3147 ( @entity3147 ). A short-term study was conducted in which @entity1068 was administered for 8 days in two liquid diets of different composition, and a chronic experiment was performed in which @entity1068 was administered for 35 days. A diet-independent 6-fold increase in @entity11920 apoprotein was observed in the short-term experiment. Expression of @entity12531 and CYP2A cross-reactive apoproteins in kidney microsomes appeared to be affected by alterations in diet but, were unaffected by @entity1068 treatment. In the chronic 35-day @entity1068 exposure experiment, @entity11920 apoprotein was also elevated 6-fold and this was found to be accompanied by a significant 3-fold increase in @entity11920 mRNA. In the same study, no @entity1068 effects were apparent on expression of CYP2B and @entity12531 apoproteins. Thus, @entity1324 induced a variety of renal cytochrome P450 forms in addition to @entity11920 , while @entity1068 appeared to be a much more specific renal @entity11920 inducer. Furthermore, as reported in the liver, @entity1324 and @entity1068 appeared to induce @entity11920 in the kidney by different mechanisms.

Expression and distribution of cytochrome P450 enzymes in male @entity35 kidney: effects of XXXX , @entity1324 and dietary conditions.

@entity1324 :: ('MESH:D000096', 'Chemical') :: ['Acetone', 'acetone'] @entity11920 :: ('25086(Tax:10116)', 'Gene') :: ['CYP2E1'] @entity3147 :: ('MESH:D044342', 'Disease') :: ['TEN', 'total enteral nutrition'] @entity12531 :: ('170509(Tax:10116)', 'Gene') :: ['CYP3A'] @entity35 :: ('10116', 'Species') :: ['rat', 'rats'] @entity11921 :: ('MESH:C024836', 'Chemical') :: ['p-nitrophenol'] @entity1068 :: ('MESH:D000431', 'Chemical') :: ['Ethanol', 'ethanol']
@entity1068 :: (MESH:D000431,Chemical) :: ['ethanol']
