UNASSIGNED: There are only a few cytogenetic analysis (CA) studies that directly compare the novel cultivation technique using immunostimulatory CpG-oligonucleotide DSP30/ @entity1199 (DSP30/ @entity1199 ) with other culture methods. Therefore, parallel cultures of peripheral blood of 129 @entity1072 ( @entity1072 ) @entity1 were set up in unstimulated cultures, in the presence of pokeweed medium (PWM), and with DSP30/ @entity1199 . Furthermore, CA results were compared with data obtained by FISH. Clonal aberrations were observed by CA in 6% of the cases in unstimulated cultures, in 27% of the cases with PWM, and in 40% of the cases with DSP30/ @entity1199 . Some clonal aberrations were detected by CA only with one culture method. Using 3 different culture methods, clonal aberrations were detected in 41% of the cases by CA and in 71% of the cases by FISH. Altogether, 78% of the cases exhibited clonal aberrations discovered by CA and FISH. Also, CA detected clonal aberrations not targeted by FISH in 7% of the cases, and FISH identified clonal aberrations not detected by CA in 36% of the cases. Our study demonstrates that the combined use of CA with different culture methods together with FISH increases our knowledge of the genetic complexity and heterogeneity in @entity1072 pathogenesis.    2014 S. Karger AG, Basel.

Detection of Clonal Aberrations by Cytogenetic Analysis after Different Culture Methods and by FISH in 129 @entity1 with XXXX .

@entity1199 :: ('3558', 'Gene') :: ['IL2', 'interleukin-2'] @entity1 :: ('9606', 'Species') :: ['patients'] @entity1072 :: ('MESH:D015451', 'Disease') :: ['CLL', 'chronic lymphocytic leukemia']
@entity1072 :: (MESH:D015451,Disease) :: ['Chronic Lymphocytic Leukemia']
