OBJECTIVE: To investigate the application of pulsed-field gel electrophoresis (PFGE) in food-borne outbreak. METHODS: Pathogens were isolated and further characteristics identified by traditional methods. The strains isolated were carried out with molecular typing with using PFGE. PFGE was performed by Laboratory Directions for molecular subtyping of @entity5746 by PFGE (CDC, USA) and the results of PFGE were analyzed by BioNumerics soft. RESULTS: Totally 14 @entity5746 serotype Muenchen strains were isolated from 19 @entity1 , 3 of 9 suspicious foods were positive for S. muenchen and 7 strains were isolated from 18 cooks. The biochemistry characterization and antimicrobial susceptibility of all the strains isolated were the same. 23 S. muenchen isolates were all shown indistinguishable by PFGE. CONCLUSION: PFGE should play a key role in identifying the outbreak-associated isolates and distinguishing them from unrelated sporadic isolates. It might also demonstrate that the genetic fingerprints of serotype Muenchen isolates derived from @entity1 were indistinguishable from those derived from drinks. PFGE might provide precise information on bacterial food-borne pathogens, promptly identify the source of @entity281 , and effectively prevent from spreading. It should be one of the early warning method on controlling outbreak of the @entity6766 .

[Application of pulsed-field gel electrophoresis in a food-borne outbreak of XXXX serotype Muenchen @entity281 ].

@entity1 :: ('9606', 'Species') :: ['patients'] @entity281 :: ('MESH:D007239', 'Disease') :: ['infection'] @entity5746 :: ('MESH:D012480', 'Disease') :: ['Salmonella'] @entity6766 :: ('MESH:D005517', 'Disease') :: ['food-borne disease']
@entity5746 :: (MESH:D012480,Disease) :: ['Salmonella']
