Residual Effect of Chlorhexidine-alcohol Compared to Triclosan-alcohol
Condition: Infectious Diseases
MeSH terms: Communicable Diseases; Infections
Intervention: Bacterial culture of the prepared skin's areas with two antiseptics and two controls; Preparing skin's areas to be tested with two antiseptics and two controls
Intervention description: Cultures will be taken with a scrub-cup of 5 cm2 of internal area pressed over the skin previously prepared with the substances, then it added a 3 mL of culture broth (D/E Neutralizing Broth, Difco TM) containing a neutralizing agent as washing solution. The skin will scrub with a sterile rubber policeman for 1 minute and the procedure will be conducted once again. Both aliquots will gather together in a sterile tube, and a sample of 50 microliters will spread in a plate containing a neutralizing agar (D/E neutralizing Agar, Difco TM) and incubate at 35°C for 24 hrs. After incubation, the colonies will be counted.; All volunteers will be provided with a neutral soap and shampoo without antiseptics for use during a period of two weeks (phase of stabilization of the skin flora). Two antiseptics (2% chlorhexidine gluconate in 70% isopropyl alcohol and 1% triclosan in 70% isopropyl alcohol) and two controls (Scrub the skin without prior application of any substance and Deionized water redistilled) will be tested as skin antiseptics. The intervention consists of preparing four skin's areas with antiseptics and controls, two in each arm of the volunteer. These ones were approximately 25 cm2 on the forearm for each antiseptic or control. The substances will be applied in an outward circular motion using a sterile swab soaked with the solutions. The solution will remain on the skin for 60 seconds, 3 hours and 24 hours before the bacterial culture will be conducted. For the control where it will be does not apply any substance, the scrub starts immediately.
Intervention MeSH terms: Ethanol; Chlorhexidine; Chlorhexidine gluconate; Triclosan; Anti-Infective Agents, Local
Phase: Phase 3
Study type: Interventional
Primary purpose: Prevention
Intervention model: Single Group Assignment
Masking: Single (Outcomes Assessor)
Enrollment: 135.0
Sex: All
Minimum age: 18 Years
Healthy volunteers: Accepts Healthy Volunteers
Keywords: Antiseptics; Administration cutaneously; Anti-infecting agents, local

Summary:

      Currently there are few options for skin antisepsis, commercially antiseptic triclosan is
      mainly used. To have more options, this study is necessary, where investigators will
      determine the residual effect of 2% chlorhexidine in 70% isopropyl alcohol and 1% triclosan
      in 70% isopropyl alcohol and choose the one with the best characteristics for skin
      antisepsis.
    

Eligibility criteria:

        Inclusion Criteria:

          -  Healthy adult volunteers

          -  Volunteers who have completed the stabilization phase of skin flora.

          -  Volunteers who does not taken a shower or bath 24 hours before the test.

        Exclusion Criteria:

          -  Volunteers with a score below 100 Colony Forming Unit per square centimeter (CFU/cm2)
             of forearm skin surface in the control after the stabilization of the skin flora.

          -  History of skin allergies or atopy, as well as reactions to alcohol, soaps, iodine,
             chlorine or latex.