Entospletinib Plus Intensive Induction/Consolidation Chemotherapy in Newly Diagnosed NPM1-mutated AML
Condition: Nucleophosmin 1-mutated Acute Myeloid Leukemia
MeSH terms: Leukemia; Leukemia, Myeloid; Leukemia, Myeloid, Acute
Intervention: Entospletinib; Placebo; Cytarabine; Anthracycline
Intervention description: 400 mg, Orally as tablets; Orally as tablets; Continuous infusion; Either daunorubicin or idarubicin was administered via slow intravenous (IV) push
Intervention MeSH terms: Cytarabine
Phase: Phase 3
Study type: Interventional
Primary purpose: Treatment
Allocation: Randomized
Intervention model: Parallel Assignment
Masking: Double (Participant, Investigator)
Enrollment: 15.0
Sex: All
Minimum age: 18 Years
Maximum age: 74 Years
Healthy volunteers: No
Keywords: Acute Myeloid Leukemia; Nucleophosmin 1-mutated Acute Myeloid Leukemia; Entospletinib; ENTO

Summary:

      The primary objective of this study is to evaluate the efficacy of entospletinib (ENTO)
      compared to placebo when added to chemotherapy in previously untreated nucleophosmin-1
      mutated (NPM1-m) acute myeloid leukemia (AML), as defined by the rate of molecularly defined
      measurable residual disease (MRD).
    

Eligibility criteria:

        Inclusion Criteria:

          1. Adults 18 to 74 years with previously untreated de novo acute myeloid leukemia (AML),
             AML with myelodysplastic syndromes (MDS) features, or therapy-related AML, who were
             candidates for intensive induction therapy.

          2. Nucleophosmin-1 (NPM1)-mutated disease documented in a local or the Sponsor's central
             testing facility.

             Note: Participants with local test results for nucleophosmin-1 mutated (NPM1-m)
             (and/or FMS-like tyrosine kinase 3 mutational status) may enroll, provided appropriate
             samples were sent to the Sponsor's central testing facility for NPM1-m companion
             diagnostic development.

          3. Eastern Cooperative Oncology Group Performance Status (ECOG PS) score of 0, 1, or 2.

          4. Adequate hepatic and renal function defined as:

               1. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) < 2.5
                  times the upper limit of normal (ULN), except those with hepatic involvement by
                  AML, as documented by either computed tomography (CT) or ultrasound, in whom
                  levels of AST and ALT < 5 times ULN are acceptable; total bilirubin < 1.5 times
                  ULN unless elevated due to Gilbert's Disease or hemolysis.

               2. Calculated creatinine clearance > 40 mL/min or serum creatinine < 1.5 times ULN.

          5. Prothrombin time (PT), activated partial thromboplastin time (aPTT), and international
             normalized ratio (INR) ≤ 1.5 x ULN unless receiving therapeutic anticoagulation.

          6. Left ventricular ejection fraction ≥ 45% confirmed by echocardiogram (ECHO) or
             multi-gated acquisition (MUGA) scan.

        Exclusion Criteria:

          1. Isolated myeloid sarcoma (ie, participants must had peripheral blood and/or bone
             marrow involvement by AML) or acute promyelocytic leukemia.

          2. Concurrent FLT3 mutation (either tyrosine kinase domain or internal tandem
             duplication).

          3. Known central nervous system (CNS) involvement with leukemia.

          4. Was a candidate for more intensive treatment than specified in this protocol.

          5. Either not a candidate for any anthracycline therapy or a candidate for induction
             therapy with a higher dose of daunorubicin (eg. 90 mg/m^2).

          6. Was a candidate for daily doses of cytarabine > 100 mg/m^2 in Induction Cycle 1.

          7. Active infection with hepatitis B, C, or uncontrolled human immunodeficiency virus
             (HIV).

          8. Known active coronavirus disease 2019 (COVID-19) either symptomatic or asymptomatic,
             as determined by nasopharyngeal swab for severe acute respiratory syndrome (SARS)
             coronavirus 2 (SARS CoV-2) ribonucleic acid (RNA) or antigen.

             Note: Participants with a history of SARS-CoV-2 nasopharyngeal carriage (either with
             or without symptoms), who had subsequently tested negative on follow-up nasopharyngeal
             swab and were without signs or symptoms of COVID-19 might enroll. Participants who
             were fully vaccinated against SARS-CoV-2 might enroll.

          9. Disseminated intravascular coagulation with active bleeding or signs of thrombosis.

         10. History of prior allogeneic hematopoietic stem cell transplant or solid organ
             transplant.

         11. Treatment with proton pump inhibitors (PPIs) from 7 days prior to enrollment until 48
             hours after completion of entospletinib (ENTO) or placebo.

             Note: PPIs were likely to interfere with ENTO absorption, thus requiring a 7-day
             washout period prior to the initiation of study medication. For management of acute
             gastrointestinal bleeding during the study treatment period (such as that related to
             chemotherapy), short term concurrent use of PPIs was permitted for up to 10
             consecutive days. If longer durations of PPI exposure were required, participants
             should discontinue study medication. Histamine (H2) receptor antagonists and antacids
             were allowed throughout the study treatment period.

         12. Ongoing immunosuppressive therapy, including systemic chemotherapy for treatment of
             leukemia.

             Note: Participants may not receive AML-directed therapy prior to enrollment other than
             hydroxyurea or leukapheresis for acute management of hyperleukocytosis.

         13. Clinical signs/symptoms of leukostasis that had failed therapy including hydroxyurea
             and/or leukapheresis of at least 3 days duration.

         14. Clinically significant heart disease defined as:

               1. New York Heart Association Class 3 or 4 congestive heart failure,

               2. Acute myocardial infarction ≤ 6 months before enrollment,

               3. Symptomatic cardiac ischemia/unstable angina ≤ 3 months before enrollment,

               4. History of clinically significant arrhythmias (eg, ventricular tachycardia or
                  fibrillation; Torsades de Pointe) including Mobitz type II 2nd degree or 3rd
                  degree heart block without a permanent pacemaker in place.

         15. Participants with a corrected congenital long measure between Q wave and T wave in the
             electrocardiogram (QT) interval (using the Fredericia formula, Fridericia correction
             of the QT measure [QTcF]) > 480 msec or Long QT Syndrome.

         16. Evidence of ongoing, uncontrolled systemic bacterial, fungal, or viral infection at
             the time of study treatment initiation, including but not limited to persistent fever
             or positive cultures in the setting of appropriate antimicrobial therapy.

         17. Unable to swallow tablets or concurrent disease affecting gastrointestinal function
             such as, malabsorption syndrome, gastric or small bowel resection, bariatric surgery,
             inflammatory bowel disease, or bowel obstruction.