To Investigate the Effects of Altering the Time of Day of Dosing (Morning or Evening) With Fluticasone Furoate 100 Micrograms Once Daily Administered Via a Dry Powder Inhaler in Subjects With Asthma
Condition: Asthma
MeSH terms: Asthma
Intervention: Fluticasone Furoate (FF); Placebo
Intervention description: Inhalation powder 100 microgram per blister strip to be administered via dry powder inhaler either in the morning (AM dose with FF and PM dose with +/-2 days).; Placebo in single strip to be administered via dry powder inhaler in the morning and evening for 14 days (+/- 2 days).
Intervention MeSH terms: Fluticasone; Xhance
Phase: Phase 2
Study type: Interventional
Primary purpose: Treatment
Allocation: Randomized
Intervention model: Crossover Assignment
Masking: Triple (Participant, Care Provider, Investigator)
Enrollment: 28.0
Sex: All
Minimum age: 18 Years
Maximum age: 70 Years
Healthy volunteers: No
Keywords: Fluticasone Furoate, FF Inhalation Powder, morning dosing, evening dosing, efficacy, FEV1

Summary:

      This study will investigate the effects of altering the time of day of dosing (morning or
      evening) with Fluticasone Furoate 100 (FF 100) micrograms (mcg) once daily administered via a
      dry powder inhaler (DPI) in subjects with persistent bronchial asthma. This is a repeat-dose,
      double-blind, randomized, placebo-controlled, three-way crossover study to compare the effect
      of morning (AM) and evening (PM) dosing with FF 100 on lung function. Twenty-four male and
      female subjects with persistent bronchial asthma will be enrolled to ensure twenty evaluable
      subjects. The three treatments will be FF 100 AM (with placebo PM), FF 100 PM (with placebo
      AM) and matching placebo (AM and PM). All treatments will be administered for 14 (+/-2) days
      with 14 day run-in and 14 to 21 day washout periods. The total duration of the study will be
      approximately 13 to18 weeks for each subject.
    

Eligibility criteria:

        Inclusion Criteria:

          -  Subjects with a documented history of persistent asthma, with the exclusion of other
             significant pulmonary diseases (pneumonia, pneumothorax, atelectasis, pulmonary
             fibrotic disease, bronchopulmonary dysplasia, chronic bronchitis, emphysema, chronic
             obstructive pulmonary disease, or other respiratory abnormalities other than asthma).

          -  Male or female between 18 and 70 years of age inclusive, at the time of signing the
             informed consent.

          -  A female subject is eligible to participate if she is:

          -  Of non-childbearing potential defined as pre-menopausal females with a documented
             tubal ligation or hysterectomy or postmenopausal defined as 12 months of spontaneous
             amenorrhea. Females on hormone replacement therapy (HRT) and whose menopausal status
             is in doubt will be required to use contraception method, if they wish to continue
             their HRT during the study. Otherwise, they must discontinue HRT to allow confirmation
             of post-menopausal status prior to study enrollment. For most forms of HRT, at least 2
             to 4 weeks will elapse between the cessation of therapy and the blood draw; this
             interval depends on the type and dosage of HRT. Following confirmation of their
             post-menopausal status, they can resume use of HRT during the study without use of a
             contraceptive method.

          -  Child-bearing potential with negative pregnancy test as determined by serum human
             chorionic gonadotropin (hCG) test at screening and serum or urine hCG test prior to
             dosing and agrees to use one of the contraception methods for an appropriate period of
             time (as determined by the product label or investigator) prior to the start of dosing
             to sufficiently minimize the risk of pregnancy at that point. Female subjects must
             agree to use contraception until completion of the follow up visit or has only
             same-sex partners, when this is her preferred and usual lifestyle.

          -  All subjects must either be clinically stable on a low to mid dose inhaled
             corticosteroid (ICS) (with or without a short acting beta-2 receptor agonist [SABA])
             such as Fluticasone Propionate (FP) 100 to250 mcg twice-daily (total daily dose 200 to
             500 mcg) or equivalent, for at least 4 weeks preceding the screening visit or be
             clinically stable on a low dose ICS/ long acting beta-2 receptor agonist (LABA)
             combination, such as SERETIDE/ADVAIR 100/50 twice- daily or equivalent (administered
             either in combination or from separate inhalers), for at least 4 weeks preceding the
             screening visit. Higher ICS/LABA doses (i.e. equivalent to SERETIDE/ADVAIR 250/50 or
             500/50) would not be acceptable. Subjects must refrain from using their LABA for at
             least 24 hour (h) prior to the screening visit

          -  Subjects with a screening pre-bronchodilator FEV1 >= 60 percent of predicted.
             Predicted values will be based upon National Health and Nutrition Examination Survey
             (NHANES III).

          -  During the screening visit, subjects must demonstrate the presence of reversible
             airway disease, defined as an increase in FEV1 of >= 12.0 percent over baseline and an
             absolute change of >= 200 mL within 60 minutes following 4 inhalations of
             albuterol/salbutamol inhalation aerosol (or equivalent nebulized treatment with
             albuterol/salbutamol solution).

          -  All subjects must be able to replace all their current asthma treatments with
             albuterol/salbutamol aerosol inhaler at screening for use as needed for the run-in
             period and throughout the duration of the study. Subjects on LABAs must also withhold
             this for at least 24 h prior to the screening visit. Subjects will therefore be on
             SABA alone for the duration of the study, and on SABA and FF during the FF treatment
             periods. Subjects must be able to withhold albuterol/salbutamol for at least 6 h prior
             to screening and study visits.

          -  Subjects who are current non-smokers and who have a pack history of <= 10 pack years.
             A subject may not have used inhaled tobacco products within the past 3 months (i.e.,
             cigarettes, cigars or pipe tobacco).

          -  Body weight >= 50 kilograms (kg) and Body Mass Index (BMI) within the range 19.0
             to29.9 kg/meter^2 (inclusive)

          -  Based on single or averaged QT duration corrected for heart rate by Fridericia's
             formula (QTcF) values of triplicate electrocardiograms (ECGs) obtained over a brief
             recording period: QTcF < 450 millisecond (msec); or QTcF < 480 msec in subjects with
             Bundle Branch Block.

          -  Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) < 2x Upper limit
             of normal (ULN), alkaline phosphatase and bilirubin <= 1.5xULN (isolated bilirubin
             >1.5xULN is acceptable if bilirubin is fractionated and direct bilirubin <35 percent).

          -  Capable of giving written informed consent, which includes compliance with the
             requirements and restrictions listed in the consent form.

          -  Able to satisfactorily use the DPI

        Inclusion Criteria for Randomisation to Treatment

          -  Evening pre-dose FEV1 of >=60 percent of their predicted normal at Day 1

          -  Daily diary compliance defined as completion of all AM daily diary data on >=4 of the
             last 7 consecutive days of the Run-in Period (not including the date of randomisation)
             and completion of all PM daily diary data on >=4 of the last 7 consecutive days of the
             Run-in Period (not including the date of randomisation)

        Exclusion Criteria:

          -  A history of life-threatening asthma, defined as an asthma episode that required
             intubation and/or was associated with hypercapnia, respiratory arrest or hypoxic
             seizures within the last 5 years.

          -  Culture-documented or suspected bacterial or viral infection of the upper or lower
             respiratory tract, sinus or middle ear that is not resolved within 4 weeks of
             screening and led to a change in asthma management or, in the opinion of the
             Investigator, is expected to affect the subject's asthma status or the subject's
             ability to participate in the study.

          -  Any asthma exacerbation requiring oral corticosteroids within 12 weeks of screening or
             that resulted in overnight hospitalization requiring additional treatment for asthma
             within 6 months prior to screening.

          -  A subject has any clinically significant, uncontrolled condition or disease state
             that, in the opinion of the investigator, would put the safety of the subject at risk
             through study participation or would confound the interpretation of the efficacy
             results if the condition/disease exacerbated during the study. The list of additional
             excluded conditions/diseases includes, but is not limited to the following: congestive
             heart failure, known aortic aneurysm, clinically significant coronary heart disease,
             clinically significant cardiac arrhythmia, stroke within 3 months of Visit 1,
             uncontrolled hypertension, recent or poorly controlled peptic ulcer, hematologic,
             hepatic, or renal disease (with the exception of Gilbert's syndrome or asymptomatic
             gallstones), immunologic compromise, current malignancy, tuberculosis (current or
             untreated), Cushing's disease, Addison's disease, uncontrolled diabetes mellitus,
             uncontrolled thyroid disorder, recent history of drug or alcohol abuse.

          -  Any adverse reaction including immediate or delayed hypersensitivity to any beta 2
             agonist, sympathomimetic drug, or any intranasal, inhaled, or systemic corticosteroid
             therapy. Known or suspected sensitivity to the constituents of the DPI (i.e. lactose).

          -  History of severe milk protein allergy.

          -  History of sensitivity to any of the study medications, or components thereof or a
             history of drug or other allergy that, in the opinion of the investigator or
             GlaxoSmithKline (GSK) Medical Monitor, contraindicates their participation.

          -  Use of prescription or non-prescription drugs including vitamins, herbal and dietary
             supplements (including St John's Wort) within 7 days (or 14 days if the drug is a
             potential enzyme inducer) or 5 half-lives (whichever is longer) prior to the first
             dose of study medication, unless in the opinion of the Investigator and GSK Medical
             Monitor the medication will not interfere with the study procedures or compromise
             subject safety.

          -  Subjects who have taken oral steroids within 12 weeks of the screening visit.

          -  History of regular alcohol consumption within 6 months of the study defined as Abuse
             of alcohol defined as an average weekly intake of greater than 21 units or an average
             daily intake of greater than 3 units (males) or defined as an average weekly intake of
             greater than 14 units or an average daily intake of greater than 2 units (females).
             One unit is equivalent to a 285 mL glass of full strength beer or 425 mL schooner of
             light beer or 1 (30 mL) measure of spirits or 1 glass (100 mL) of wine.

        Exclusion Criteria based upon diagnostic assessments

          -  A subject will not be eligible if he/she has clinical visual evidence of oral
             candidiasis at screening.

          -  Pregnant females as determined by positive serum hCG test at screening or by positive
             serum or urine hCG test prior to dosing.

          -  A positive pre-study Hepatitis B surface antigen or positive Hepatitis C antibody
             result within 3 months of screening.

          -  Urinary cotinine levels indicative of smoking or history or regular use of tobacco- or
             nicotine-containing products within 3 months prior to screening.

          -  A positive pre-study drug/alcohol screen.

          -  A positive test for human immunodeficiency virus (HIV) antibody. Other Criteria

          -  Lactating females.

          -  Where participation in the study would result in donation of blood or blood products
             in excess of 500 mL within a 56 day period.

          -  The subject has participated in a clinical trial and has received an investigational
             product (IP) within the following time period prior to the first dosing day in the
             current study: 30 days, 5 half-lives or twice the duration of the biological effect of
             the investigational product (whichever is longer).

          -  Exposure to more than four new chemical entities within 12 months prior to the first
             dosing day.

          -  No subject is permitted to perform night shift work for 1 week prior to screening
             until completion of the study treatment periods.

          -  Unwillingness or inability to follow the procedures outlined in the protocol.

        Exclusion Criteria for Randomisation to Treatment

          -  Resuming their asthma medication (excluding albuterol/salbutamol inhalation aerosol
             provided at screening).

          -  Occurrence of a culture-documented or suspected bacterial or viral infection of the
             upper or lower respiratory tract, sinus or middle ear during the run-in period that
             led to a change in asthma management or, in the opinion of the Investigator, is
             expected to affect the subject's asthma status or the subject's ability to participate
             in the study.

          -  Evidence of a severe exacerbation, defined as deterioration of asthma requiring the
             use of systemic corticosteroids (tablets, suspension or injection) for at least 3 days
             or an in-patient hospitalization or emergency department visit due to asthma that
             required systemic corticosteroids between screening and Day 1.

          -  Clinical visual evidence of oral candidiasis at Day 1.