Relative Bioavailability Study of Marketed and Lower Dose Ambrisentan in Healthy Adult Participants
Condition: Hypertension, Pulmonary
MeSH terms: Hypertension, Pulmonary; Hypertension
Intervention: AMB new formulation (1 mg); Reference AMB (5 mg)
Intervention description: AMB tablets will be available at a unit dose strength of 1 mg. Participants will orally administer 5 tablets of 1 mg unit dose.; AMB reference tablet will be available as film-coated tablet at unit dose strength of 5 mg. Participants will orally administer 1 tablet of 5 mg unit dose
Phase: Phase 1
Study type: Interventional
Primary purpose: Treatment
Allocation: Randomized
Intervention model: Crossover Assignment
Intervention model description: This is a three-period crossover study
Masking: None (Open Label)
Enrollment: 29.0
Sex: All
Minimum age: 18 Years
Maximum age: 65 Years
Healthy volunteers: Accepts Healthy Volunteers
Keywords: Ambrisentan; Oral dosage forms; Pulmonary arterial hypertension; Bioavailability

Summary:

      This is a single center, open-label, randomized, single-dose, three-period cross-over study
      in healthy participants. The aim of this study is to provide clinically relevant information
      on the pharmacokinetic (PK) and safety profile of a new lower dose formulation ambrisentan
      (AMB) tablet, which is intended for pediatric use. The study will compare the relative
      bioavailability of the lower dose tablet, dispersed in water and administered orally, with
      the reference marketed AMB tablet in healthy adults. The total study duration for each
      participant is expected to be approximately 9 weeks.
    

Eligibility criteria:

        Inclusion Criteria:

          -  Participants must be 18 to 65 years of age inclusive, at the time of signing the
             informed consent.

          -  Participants who are overtly healthy as determined by medical evaluation including
             medical history, physical examination, laboratory tests, vital signs and cardiac
             monitoring.

          -  Average systolic blood pressure between 100-160 millimeter of mercury (mmHg) and
             diastolic between 55-90 mmHg (inclusive) over 3 readings at Screening.

          -  Body weight >=50 kilogram (kg) for men and >= 45kg for women, and body mass index
             (BMI) within the range 18-30 kilogram per meter square (kg/m^2) (inclusive).

          -  Male participants are eligible to participate if they agree to the following during
             the study and for at least 13 weeks afterwards corresponding to time needed to
             eliminate study intervention (5 terminal half-lives) plus an additional 90 days (a
             spermatogenesis cycle): 1. Refrain from donating sperm plus either 2. Be abstinent
             from heterosexual intercourse as their preferred and usual lifestyle (abstinent on a
             long term and persistent basis) and agree to remain abstinent. OR

          -  Must agree to use contraception/barrier, as follows: Agree to use a male condom; and
             Female partner to use an additional highly effective contraceptive method with a
             failure rate of <1% per year as described.

          -  A female participant is eligible to participate if she is not a woman of childbearing
             potential (WOCBP).

          -  Capable of giving signed informed consent.

        Exclusion Criteria:

          -  History or presence of cardiovascular, respiratory, hepatic, renal,
             gastrointestinal,endocrine, hematological, or neurological disorders capable of
             significantly altering the absorption, metabolism, or elimination of drugs;
             constituting a risk when taking the study intervention or interfering with the
             interpretation of data.

          -  History or presence of palpitations or tachyarrhythmia.

          -  Hemoglobin (Hb) below the normal range (Hb <133 grams per liter [g/L] for male
             participants ; and Hb <114 g/L for female participants).

          -  Alanine transaminase (ALT) >1.5 times upper limit of normal (ULN)

          -  Bilirubin >1.5 times ULN (isolated bilirubin >1.5 times ULN is acceptable if bilirubin
             is fractionated and direct bilirubin <35%).

          -  Current or chronic history of liver disease, or known hepatic or biliary abnormalities
             (with the exception of Gilbert's syndrome or asymptomatic gallstones).

          -  Corrected QT interval (QTc) >450 millisecond (msec).

          -  Past or intended use of over-the-counter or prescription medication (including
             vitamins and dietary or herbal supplements but excluding paracetamol <=2 grams/day)
             within 7 days (or 14 days if the drug is a potential enzyme inhibitor) or 5 half-lives
             (whichever is longer) prior to the first dose of study medication, unless approved by
             the Investigator in conjunction with GlaxoSmithKline Medical Monitor.

          -  Participation in the study would result in loss of blood or blood products in excess
             of 500 milliliter (mL) within a 56-day period.

          -  Exposure to more than 4 new chemical entities within 12 months prior to the first
             dosing day.

          -  Current enrolment or past participation within 30 days before Screening in any other
             clinical study involving an investigational study intervention or any other type of
             medical research.

          -  Presence of Hepatitis B surface antigen (HBsAg) at Screening or within 3 months prior
             to first dose of study intervention.

          -  Positive Hepatitis C antibody test result at Screening or within 3 months prior to
             first dose of study intervention.

          -  Positive Hepatitis C Ribonucleic acid (RNA) test result at Screening or within 3
             months prior to first dose of study intervention.

          -  Positive human immunodeficiency virus (HIV) antibody test.

          -  Positive pre-study drug/alcohol screen.

          -  Regular use of known drugs of abuse.

          -  Regular alcohol consumption within 6 months prior to the study defined as: An average
             weekly intake of >14 units. One unit is equivalent to 8 grams of alcohol: a half-pint
             (equivalent to 240 mL) of beer, 1 glass (125 mL) of wine or 1 (25 mL) measure of
             spirits.

          -  Smoking > 5 cigarettes per week (or equivalent) and participants must be able to
             abstain from smoking for a 24-hour period prior to dose and any time whilst in the
             clinical unit.

          -  Sensitivity to any of the study interventions, or components thereof, or drug or other
             allergy that, in the opinion of the investigator or medical monitor, contraindicates
             participation in the study