The Research of Metabolomics on COPD
Condition: Metabolomics; Omega 6; Omega 3; COPD
Study type: Observational

Summary: 
      Targeted metabolomics research was conducted on patients with chronic obstructive pulmonary
      disease (COPD). The correlation analyst between inflammation-related metabolites and lung
      function was analyzed by explored the metabolic pathways of inflammation-related omega-6 and
      omega-3 and combining them with lung function.
    

Detailed description: 
      Based on the global initiative for chronic obstructive pulmonary disease, the 80 confirmed
      stable COPD patients with mild-to-moderate were involved, 80 COPD patients with severe and
      very severe, and age, sex, all matching 40 cases without lung disease. The participants
      information was collected. Whole blood and pulmonary function were examined at three time
      points: admission, six months and one year, at the same time, targeted metabolomic analysis
      method are applied to detect serum metabolites, According to the access to assess the
      different severity of COPD in the stable period and the level of metabolites in different
      admission periods. After that, correlation analysis was conducted with pulmonary function
      indicators and other clinical indicators to observe the research value of
      inflammation-related metabolites in the development of COPD.
    

Intervention name(s): Lung function test was performed on the subjects with Jaeger lung function instrument of Germany.; Agilent 1290 Infinity LC system (UHPLC, Santa Clara CA) was used for Targeted metabolite detection.; Routine blood test
Intervention type(s): Diagnostic Test; Diagnostic Test; Diagnostic Test
Intervention description(s): This Jaeger lung function instrument of Germany is used to test the pulmonary ventilation function of COPD patients. Since the condition requires drug control and treatment, the drug cannot be stopped.; We used the Agilent 1290 Infinity LC system mass spectrometer to analyze and detect metabolite peaks of patients according to retention time and mass/charge ratio after preliminary experiments and derivatization.; Blood routine test results include CRP, PCT, white blood cell count, red blood cell count, neutrophil percentage, eosinophil, basophil, et al.
Observational model: Case-Control
Time perspective: Retrospective
Sampling method: Probability Sample
Study population: 
        Based on the global initiative for chronic obstructive pulmonary disease, the 80 confirmed
        stable COPD patients with mild-to-moderate were involved, 80 COPD patients with severe and
        very severe, and age, sex, all matching 40 cases without lung disease. The participants
        information was collected. Whole blood and pulmonary function were examined at three time
        points: admission, six months and one year, at the same time, targeted metabolomic analysis
        method are applied to detect serum metabolites, According to the access to assess the
        different severity of COPD in the stable period and the level of metabolites in different
        admission periods.
      
Keywords: Metabolomics; Omega 6; Omega 3; COPD; lung function tests