Influence of Dietary Fiber-rich Meals on Gene Expression and Postprandial Glucose and Lipid Response
Condition: Hypoglycemia; Hyperglycemia
MeSH terms: Hypoglycemia; Hyperglycemia
Study type: Interventional

Summary: 
      The aim of this study is to

        -  Measure the effect on gene expression in leukocytes from a meal rich in oat bran

        -  Investigate the postprandial glucose, insulin and triglyceride responses after intake of
           meals containing fiber from different sources (oat, rye and sugar beet fiber) or a meal
           containing a mixture of these three fibers
    

Detailed description: 
      Dietary fiber has long been known to give beneficial health effects. Yet, the understanding
      of how fiber-rich meals regulate molecular events at a gene level is limited. Also, few
      studies have compared the effects of different fiber sources on postprandial responses and
      hardly any study the effects of fiber mixtures in the same meal, even though this is more
      similar to regular eating habits.

      Healthy subjects will come to the study center after an overnight fast, to ingest breakfasts
      randomly enriched with different fiber. The meals contains either spray-dried oat drink, rye
      bran, sugar beet fiber a mixture of these three fibers, oat bran or no added fiber (control).
      All meals are adjusted to contain the same total amount of available carbohydrates and fat.
      Blood leukocytes for gene expression profiling were taken before and 2 h after consumption
      while blood samples for analysis of postprandial glucose, insulin and triglyceride levels
      were taken every 30 min during 3 h.

      NuGO Affymetrix Human Genechip NuGO_Hs1a520180 are used for the microarray analysis and
      analysis is performed with linear mixed models and enrichment analysis to identify functional
      gene sets that responded to the specific oat bran effect.
    

Intervention name(s): Oat bran; Spray-dried oat drink; Rye bran; Sugar beet fiber; Mixture of fiber; Control
Intervention type(s): Other; Other; Other; Other; Other; Other
Intervention description(s): 82 g oat bran was added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (12.6 g total fiber).; 62 g spray-dried oat drink was added to 250 ml blackcurrant beverage with pulp to give 2.7 g soluble fiber (3.3 g total fiber).; 31 g rye bran was added to 250 ml blackcurrant beverage with pulp to give 1.7 g soluble fiber (12 g total fiber).; 19 g sugar beet fiber was added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (12 g total fiber).; 38 g spray-dried oat drink, 30 g rye bran and 6 g sugar beet fiber were added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (18 g total fiber).; No fiber was added to control meal (250 g black-currant beverage)
Other intervention name(s): Oats; Beta-glucan; Oats; Oat milk; Beta-glucan; Rye; Arabinoxylan; Sugar beet; Pectin
Allocation: Randomized
Intervention model: Factorial Assignment
Masking: Single (Participant)
Keywords: gene expression; oats; rye; sugar beet fiber; glucose; insulin; triglyceride; decreased postprandial glucose; decreased postprandial insulin; glycemic index