Fetal Alcohol Spectrum Disorder-Is This a Ciliopathy?
Condition: Fetal Alcohol Spectrum Disorder
MeSH terms: Fetal Alcohol Spectrum Disorders; Ciliopathies
Study type: Interventional

Summary: 
      Urine sample and exhaled Nitric Oxide will analyzed and compared between children diagnosed
      with Fetal Alcohol Spectrum Disorder and healthy control.

      Pilot study- 5 children in each group
    

Detailed description: 
      Background: The term "fetal alcohol spectrum disorder" (FASD) describes a broad spectrum of
      neurodevelopmental presentations resulting from alcohol exposure in utero. The prevalence has
      been estimated at 1 in 100 people, and estimated to be more than 330 000 affected individuals
      in Canada. The Canadian Diagnostic Guidelines describe the physical and neurodevelopmental
      effects resulting from prenatal alcohol exposure. These include distinct facial features,
      microcephaly and neurodevelopmental deficits.

      Ethanol toxicity is researched in association to the concentration, duration, and timing of
      ethanol exposure and the possible pathways leading to phenotypic translation to
      teratogenesis. Studies of FASD in animal models are beginning to implicate a number of
      susceptibility genes that are involved in various molecular pathways on gene-ethanol
      interactions. Some of which are reportedly involved in ethanol teratogenesis as a part of
      their roles in DNA damage control, Central Nervous System axis formation, cellular survival
      as well as proliferation and growth.

      A mutation in one particular gene, neuronal nitric oxide synthase (nNOS)) is known to worsen
      alcohol induced neuronal death both in vivo and in vitro, and expression of the nNOS gene
      protects neurons against alcohol toxicity Nitric Oxide (NO) is synthesized by the enzyme
      nitric oxide synthase (NOS through) L-arginine There are three NOS isoforms: neuronal NOS
      (nNOS, NOS-1), inducible NOS (iNOS, NOS-2), and endothelial NOS (eNOS, NOS-3) .NO is
      associated with modulation of neurotransmission and memory formation along with other vital
      activities. In the presence of O2, radicals form that can lead to toxicity. They may be
      disruptive to cell signaling processes and result in pathological conditions.

      Data suggest that ethanol alters NOS expression and activity in the brain There are known
      effects of ethanol on production of NO, NOS activities, and interactions of NO with alcohol
      metabolism enzymes.

      Dose and length of ethanol exposure are the main factors determining ethanol effects on NO
      production, NOS activity or NOS expression.

      Structural congenital brain anomalies are described in the context of FASD including
      microcephaly, migration anomalies, neural tube defects along with midline brain anomalies
      involving the corpus callosum, cerebellar anomalies and hydrocephalus.

      Cilia are evolutionarily-conserved antennae-like organelles found on the surface of cells.
      Cilia detect different extracellular stimuli such as fluid flow, light, smell, and hormones
      through specific ciliary receptors, and they play key roles in cell locomotion and division,
      fluid movement, as well as embryonic development of different tissues. In regards to neural
      development, ciliary growth factors were recently shown to play a role in embryonic neuronal
      cell migration and signaling as well as in formation of the Central Nervous System (CNS)
      midline and lateralization. In the mature organism, cilia extend from the basal bodies of the
      ependymal cells lining the ventricular surface of the brain, where they facilitate the flow
      of Cerebro Spinal Fluid (CSF).

      Nervous system defects are strongly associated with a number of ciliopathies, with common
      findings including midline defects such as hydrocephalus, neural tube defects, and a plethora
      of cortical and cerebellar abnormalities.

      The prototype human ciliary disease has been Primary Ciliary Dyskinesia (PCD). In the past
      decade, significant advancement in understanding of PCD has led to improved diagnosis, mainly
      by the finding that Nasal nitric oxide (nasal NO) is greatly reduced in these patients. The
      mechanism of this reduction has been suggested to be related to reduced iNOs expression as
      part of the ciliopathy. Nasal NO measurement is a simple non-invasive test easily carried out
      even in the youngest patients.

      As NO measurement is a simple non-invasive test with immediate results, it serves as an ideal
      screening test for suggestive diagnosis of various ciliopathies, including those involving
      brain malformations.

      In recent work, this research group was the first to determine a connection between ciliary
      dysfunction as measured by nasal NO concentration and isolated midline central nervous system
      (CNS) anomalies The overall mean nasal NO level in children with isolated midline CNS
      anomalies in the study, was significantly lower compared to the previously-established normal
      range with some being within the range of Primary Ciliary Dyskinesia (PCD). Recent work shows
      that inter neuronal connectivity, brain ventricles morphogenesis and proper formation of the
      neural tube depend on non-disrupted ciliary function.

      The primary cilia function as a result of membrane proteins such as polycystin-1 (PC-1),
      PC-2, TRPV4, P2Y12, and fibrocystin. Acetylated alpha tubulin is a known ciliary marker.

      Alterations in PC-1 are associated with polycystic kidney disease type 1 mutations that were
      found to be associated with fatty acid oxidation defects in metabolomic and lipidomic tests.
      Acetylated alpha tubulin is assessed through proteinomic analysis.

      Hypothesis: Given the recent recognition of the interactions between NO with ethanol and the
      important role of cilia in early brain development, the investigators hypothesize that cilia
      may be involved in molecular pathways leading to brain injury in children that are diagnosed
      with FASD. Such a mechanism has not been previously explored.

      The investigators hypothesize that nasal NO levels as well as the key metabolites in patients
      diagnosed with FASD will be reduced compared to healthy controls.

      Objective: This study will aim at measuring nasal NO in a selected group of patients
      diagnosed with Fetal Alcohol Spectrum Disorder, along with metabolomics and proteinomic
      analysis as related to key metabolites involved in NO pathway in urine samples, and compare
      them to healthy children. This study will enable clinicians to potentially employ a
      non-invasive screening tool for pediatric patients with suspected Fetal Alcohol Spectrum
      Disorder and consider an early developmental intervention.

      Methods: 10 children ages 5-16 years, who are diagnosed with FASD will be recruited from the
      FASD outpatient clinic at the Glenrose Rehabilitation Hospital. 10 healthy children of the
      same age range will be recruited through the HICUPP registry for healthy children
      (Pro00056156).

      The investigators will measure nasal NO levels by inserting an inert NO sampling line with a
      disposable foam olive (DirectMed Inc., Glen Cove, NY) into the child's nostril while the
      contralateral nostril is left open. Air will be then sampled at a constant rate of 0.3
      liters/min from the nose by a chemiluminescent analyzer which provides measurement of the
      nasal NO level in parts per billion (ppb). All nasal NO measurements will be performed with
      the subjects seated.

      Measurements will be obtained using an NO analyzer (CLD 88 SP, ECO PHYSICS AG, Duerten,
      Switzerland). The analyzer will be calibrated according to the manufacturer's specifications.
      Measurements will be made using velum-closure technique via exhalation from a deep
      inspiration through a fixed resistor (disposable, cardboard cylinder with 1 mm opening;
      DirectMed Inc., Glen Cove, NY) for 20-40 seconds or via a party favor blowout toy with a
      comparable expiratory resistance. Both types of resistor require slight puffing of the cheeks
      to develop a mouth pressure > 5 cm H2O, a pressure sufficient to close the soft palate. The
      maneuvers will be performed according to ATS/ERS guidelines. In patients who are
      uncooperative with the above maneuver the measurements will be made using tidal breath
      sampling.

      The examiner will enter the measured NO level directly into a computer file, which will be
      saved in an encrypted format on a password-protected computer at the Glenrose Rehabilitation
      Hospital. Identifying information such as participants' names or phone numbers will not be
      entered. Each participant will be assigned a number instead of a name, which will be
      recorded. Other identifying information, such as the child's gender, age and diagnosis, will
      be coded using a number as well.

      Based on information provided, a statistical analysis of co-variants will be performed,
      comparing the measured NO levels of patients with FASD to healthy controls (with the
      reference range of the normal as published in Mateo 2011 and Marthin 2011).

      In addition to the nasal NO measurements, the investigators will also conduct metabolomic and
      proteomic analysis to measure the levels of key metabolites and proteins in urine of patients
      with FASD. These will be compared to the healthy, controls to further explore this hypothesis
      of ciliary involvement. The metabolomic analyses will be used to measure key metabolites
      involved in the NO pathway (arginine, citrulline, ornithine, asymmetric dimethylarginine) as
      well as soluble NO byproducts (nitrotyrosine, nitrotryptophan, and
      3-nitro-4-hydroxyphenylacetic acid). Additional metabolomic studies will be performed to look
      for altered beta-oxidation markers (acylcarnitines) which can be affected by ciliary
      dysfunction, vitamin levels (esp. vitamin A and its byproducts) as well as changes in
      catecholamines (due to altered neuronal functions affected by cilia) in urine. The
      metabolomic analyses will be done using targeted, MS-based metabolomics methods. The
      investigators will also conduct proteomic analysis on urine in FASD patients to measure
      changes in key ciliary proteins, namely polycystin-1 (PC-1)( PC-2, TRPV4, P2Y12, fibrocystin)
      as well as acetylated alpha-tubulin and compare these values to a group of healthy children.
      The proteomic analyses will consist of a combination of MS-based proteomics and immunoassays.

      Data will be analyzed using PASW Statistics Version 19 (SPSS Inc., 2010). Alpha will be set
      at .05 for all analyses. The percentage of children who score above and below age and
      norm-based cut-off values on each measurement will be computed.

      To determine whether the two groups (FASD, Healthy controls) differ in the measured level a
      Multivariate Analysis of Variance (MANOVA) will be conducted on subscale scores using Group
      as a between-subjects factor.

      Noting that the sample size is small, the investigators will report an effect size between
      the groups for the NO level, and the metabolomics analysis, as well as a 95% confidence
      interval for the effect size. The hypothesis is that there is a difference between the
      groups; given that the investigators cannot prove the null (i.e., a lack of a statistically
      significant finding will not prove the hypothesis), the investigators will use this effect
      size to instead estimate the effect. However it is hypothesized that the nasal NO levels and
      key metabolites as relates to NO in children with FASD will be reduced in comparison to
      healthy controls.

      Following the completion of the study, the de-identified data will be kept in an encrypted
      file on the password-protected computer for a minimum of five years.
    

Intervention name(s): Metabolomic analysis urine sample
Intervention type(s): Diagnostic Test
Intervention description(s): a urine sample will be anlayzed for metabolic pathways involving Nitric Oxide Nitric Oxide level will be measured at the exhaled air sample
Other intervention name(s): NO- Analyzer breath test
Intervention model: Single Group Assignment
Intervention model description: pilot. 5 children with FASD- 5 healthy children
Masking: None (Open Label)
Primary purpose: Basic Science