Effects of the Consumption of Broccoli Sprouts in Overweight Subjects
Condition: Obesity; Inflammation
MeSH terms: Overweight; Inflammation
Study type: Interventional

Summary: 
      Nowadays there is an increasing demand by consumers on healthy food products prepared in
      convenient forms, simple to use and not containing additives. In this sense, broccoli sprouts
      (Brassica oleraceae var. italica) represent an interesting choice as they are rich in
      glucosinolates, nitrogen-sulfur compounds, that are believed to counteract the negative
      effects of diverse pathologies.

      Human studies have been focused on the antitumoral properties of these vegetables, however
      there is less evidence on the anti-inflammatory properties of cruciferous vegetables in
      humans.

      As obesity is linked to an inflammatory component, the aim of the study is to evaluate the
      anti-inflammatory action of broccoli sprouts in overweight adult subjects.
    

Detailed description: 
      We performed an interventional follow-up study to evaluate the effect of the daily
      consumption of broccoli sprouts during 10 weeks (70 days). The study was conducted at the
      Catholic University of Murcia under supervision of Consejo Superior de Investigaciones
      Científicas (CSIC).

      The study was performed according with the Helsinki Declaration of Human Studies and approved
      by the Ethical Committee of the Catholic University of Murcia as well as the Bioethics
      Sub-Committee of the CSIC' Department of Ethics for the AGL-2013-46247-P project. Volunteers
      (n=40; 21 M, 19 W) were recruited in the Catholic University of Murcia (UCAM) and all of them
      were informed on the characteristics of the study and they signed the written-informed
      consent. Dietetic and life style habits were recorded from all participants. There were no
      drop-outs during the whole period of study and no adverse effects were reported due to the
      broccoli sprout ingestion.

      One week before the beginning of the intervention period, subjects were asked to avoid the
      consumption of Brassica vegetables (broccoli, radish, cauliflower, Brussel sprouts, mustards,
      among others) and their derived products, and to follow a well-balanced diet (based on
      Mediterranean diet), with no other food restriction criteria. These dietary instructions were
      maintained during the entire period of study. Besides, they were requested to record any sign
      of adverse effect, illness or deviation of the experimental diet. The subjects maintained
      their usual lifestyles during the study.

      On the first day, participants were given the portions of fresh broccoli sprouts to be taken
      for the whole week (7 trays of broccoli sprouts of 30 g each) and each week they had an
      appointment to provide them the fresh products. The intervention consisted on a 10-week
      period which included daily consumption of a portion (30 g) of raw, fresh broccoli sprouts.
      This amount is consistent with a half- serving according to WHO. Subjects were instructed to
      ingest 1 tray per day and to keep the trays refrigerated (4º C) at home. The intake of the
      broccoli sprouts was included in their normal daily diet and no specific time of consumption
      was established, with the only limitation of avoid cooking of the sprouts and to consume them
      fresh. Cooking procedures can affect the content of glucosinolates as well as their
      bioavailability and therefore some recipes were provided to the participants to facilitate
      the intake of the sprouts without affecting the phytochemical composition and absorption.
      After the intervention period, a follow-up recovery period for all subjects continued for
      other 90 days with no ingestion of broccoli sprouts.

      Fasting blood samples and 24-h urine samples were taken on day 0 (jst before starting the
      intervention), day70 (end of intervention period), day 90 (20 days after end of intervention)
      and day 160 (90 days after end of intervention). Blood samples were collected from each
      subject by venipuncture from the antecubital vein; 3 mL were placed in heparin tubes and
      centrifuged at 10000 rpm for 10 min at 4ºC. Plasma was aliquoted and stored at -80ºC until
      analysis. Analysis were performed once each period was finished and in the same batch to
      minimize analytical variations. The total volume of the 24h-urine was recorded to calculate
      the absolute amounts of the compounds and metabolites excreted in the study period and
      aliquots were frozen at -80ºC for further analysis. Body weight and percentage of fat mass
      were measured as well and BMI calculated in each sampling time point.

      Markers of inflammation as IL-6, C-reactive protein, IL-1β and TNF-α in plasma were
      determined in our laboratory using high-sensitivity ELISA kits.

      Levels of glucosinolates, isothiocyanates and their metabolites were measured in urine by a
      rapid, sensitive and high throughput UHPLC-QqQ-MS/MS.
    

Intervention name(s): Broccoli sprouts
Intervention type(s): Other
Intervention model: Single Group Assignment
Intervention model description: The intervention consisted on a 10-week period which included daily consumption of a portion (30 g) of raw, fresh broccoli sprouts. After the intervention period, a follow-up recovery period for all subjects continued for other 90 days with no ingestion of broccoli sprouts.
Masking: None (Open Label)
Primary purpose: Prevention
Keywords: IL-6; Glucosinolate; Brassica oleracea; Sulphoraphane; Biomarkers; Bioavailability; Inflammation