Resistance Exercise and Hydrolyzed Collagen Supplementation
Condition: Diet, Food, and Nutrition
Study type: Interventional

Summary: 
      This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC)
      with resistance exercise (RE) on whole body collagen synthesis in healthy young and older
      males and females.
    

Detailed description: 
      This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC)
      with resistance exercise (RE) on whole body collagen synthesis in healthy young and older
      males and females.

      Healthy young and older males and females ingested 0 grams, 15 grams or 30 grams HC with 50
      milligrams vitamin C 1h prior to performing four sets' barbell back-squat RE at 10-repetition
      maximum load, after which they rested for six hours. Blood samples were collected throughout
      each trial to analyse procollagen type Ⅰ N-terminal propeptide (PⅠNP, a biomarker of collagen
      synthesis) and β-isomerized C-terminal telopeptide of type I collagen (β-CTX, a biomarker of
      collagen breakdown) concentration, and the concentration of 18 amino acids that constitute
      collagen.

      This is the first study to investigate the combined effect of different doses HC with
      high-intensity RE on whole body collagen synthesis in healthy young and older men and women.
      If 30 grams HC intake with RE does augment collagen synthesis more than RE alone, this
      suggests that long-term HC intake with chronic RE would be beneficial for tendon health,
      because collagen is the most abundant protein in tendon.
    

Intervention name(s): Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise; Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise
Intervention type(s): Dietary Supplement; Dietary Supplement
Intervention description(s): Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula.
All trials were performed at the same time of day (08:00 - 15:00).
Different doses of HC (0 grams, 15 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams and 15.4 grams maltodextrin was used in 0 grams HC and 15 grams HC respectively. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses.
The details of nutritoinal supplements used are as follows:
Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK); Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula.
All trials were performed at the same time of day (08:00 - 15:00).
Different doses of HC (0 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams maltodextrin was used in 0 grams HC. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses.
The details of nutritoinal supplements used are as follows:
Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK)
Other intervention name(s): Resistance exercise and collagen dose-response for male participants; Resistance exercise and collagen dose-response for female participants
Allocation: Randomized
Intervention model: Crossover Assignment
Intervention model description: A double-blind, randomised cross-over design
Masking: Triple (Participant, Investigator, Outcomes Assessor)
Primary purpose: Basic Science
Keywords: Hydolyzed collagen; Resistance exercise; Collagen synthesis; Proline; Glycine