Circulatory Levels of Irisin in Response to Chronic Resistance Training
Condition: Healthy
Study type: Interventional

Summary: 
      Study the responses of circulatory irisin to chronic high-intensity resistance training
    

Detailed description: 
      Objective: The purpose of this study is to study the circulating levels of irisin in response
      to chronic high-intensity resistance training in a group of healthy young adults.

      Sample: The sample will be comprised 44 of adult men and women unpaid volunteers between the
      ages of 18 and 30.

      Pre-screening assessments:

      Informed consent, PAR-Q, Medical History and Exercise History will be used to determine
      health status and prior exercise experience. Menstrual cycle log will be required for female
      participants.

      Pre-Intervention assessments:

        -  Anthropometric measurements and Body Composition: Height, triceps, thigh and calf
           skinfolds, biceps, thigh, hip, waist, and calf circumferences will be measured.
           Dual-energy x-ray absorptiometry (DEXA) will be performed to obtain body composition.

        -  Cardiopulmonary and Strength Fitness Testing. Cardiopulmonary fitness will be determined
           via maximum oxygen uptake testing. One maximum repetition (1-RM) will be used to assess
           the muscular fitness.

        -  Spontaneous Physical Activity: Daily activity level for 7 days will be collected.

        -  Familiarization. Participant will be instructed on the muscle conditioning techniques
           used for strength training so that the techniques can be mastered. The rate of perceived
           exertion (RPE) scale will also be used for familiarization during these sessions.

      Intervention: The intervention will last 3 weeks with a frequency of 3 training session per
      week. We will collect blood samples levels at session 1 (at the beginning of week 1), session
      3 (at the end of week 1), at session 6 (at the end of week 2) and session 9 (at the end of
      week 3). Blood draws will occur at the beginning of each session evaluated (pre-session), at
      the 45 min mark during each session and immediately after each session (post-session).

      The sessions will consist on a circuit training of 3 sets of 7 exercises focused on major
      muscle groups. Ten repetitions on each circuit at 70% of the maximum capacity will be
      performed.

      During the intervention:

      - Blood-related variables. Baseline glucose, lactate and hematocrit will be obtained from all
      participants prior to intervention. Lactate and Hematocrit will be obtained pre-intervention
      and post-intervention, and within 1 min post workout at sessions 1, 3, 6 and 9.

      Irisin will be obtained from blood samples at baseline, during and after training sessions 1,
      3, 6 and 9. Irisin will be measured via a commercial enzyme-linked immunosorbent assay.

      - Dietary Controls. Each participant will complete the Food Preference Questionnaire. In
      addition, the Automated Self-Administered 24-hour Recall (ASA24) will be collected for the
      days prior to sessions 1, 3, 6 and 9.

      Post-Intervention assessments:

        -  Anthropometric measurements and Body Composition: Height, triceps, thigh and calf
           skinfolds, biceps, thigh, hip, waist, and calf circumferences will be measured.
           Dual-energy x-ray absorptiometry (DEXA) will be performed to obtain body composition.

        -  Caridorespiratory capacity determined via maximum oxygen uptake testing. One maximum
           repetition (1-RM) will be used to assess the muscular fitness.

        -  Spontaneous Physical Activity: Daily activity level for 7 days will be collected.
    

Intervention name(s): Exercise Training
Intervention type(s): Other
Intervention description(s): Chronic High-Intensity Resistance Training
Allocation: Randomized
Intervention model: Parallel Assignment
Masking: None (Open Label)
Primary purpose: Basic Science
Keywords: Volunteers