Bioavailability of Different Collagen-based Treatments After Oral Intake
Condition: Healthy Volunteers
Study type: Interventional

Summary: 
      The goal of this clinical trial is to investigate the postprandial absorption of collagen and
      elucidate the absorption rate and bioavailability after oral intake of collagen-based
      products. Participants will be asked to drink solutions containing the study products (one
      product per group, a total of four products), and blood samples will be obtained by digital
      puncture at different time points. Plasma will be collected and a set of collagen-derived
      amino acids, di-peptides and tri-peptides will be quantified by LC-MS. Each product will be
      compared to the placebo group.
    

Detailed description: 
      Collagen is characterized by its high content of glycine, proline and hydroxyproline, and it
      is found to exert beneficial effects on several health benefits. Recent evidence suggests
      that the consumption of amino acids and/or peptides abundantly present in collagen may have
      the capacity to influence the synthesis of new collagen in the body. Indeed, it has been
      found that collagen supplements improve skin moisture, elasticity, and hydration when orally
      administered. Additionally, collagen reduces the wrinkling and roughness of the skin, and
      existing studies have not found any side effects of its oral supplements. Thus, to exert any
      beneficial effects, it is essential that hydrolyzed collagen is optimally absorbed. This
      study aimed to investigate the postprandial absorption of collagen and elucidate the
      absorption rate and bioavailability after oral intake.

      The study personnel will prepare the drinkable solutions containing the study products in a
      room accessible to site staff only. Drinkable solutions will be provided to subjects just
      before consumption. Product ingestion will be early in the morning after overnight fasting
      for 12 h. On the morning of the experiment, subjects will fast and each subject will take the
      products orally. Dosage (Quantity of product to consume orally): This is calculated depending
      on the body weight; 400 mg/kg of body weight with 20 w/v % water. Collagen products (fine
      powder) will be diluted in water prior consumption. Control group ingest approximately same
      amount of plain water. Participants cannot drink water within 4 hours before and after the
      product consumption.

      The assessment of the bioavailability of the four collagen-based treatments (CP1, CP2, CP3,
      CP4) will be evaluated by quantifying 8 di/tri-peptides and the amino acid profile in the
      blood of 25 volunteers by LC-MS.
    

Intervention name(s): Dietary supplementation with placebo.; Dietary supplementation with the collagen-based product CP1.; Dietary supplementation with the collagen-based product CP2.; Dietary supplementation with the collagen-based product CP3.; Dietary supplementation with the collagen-based product CP4.
Intervention type(s): Dietary Supplement; Dietary Supplement; Dietary Supplement; Dietary Supplement; Dietary Supplement
Intervention description(s): Volunteers will be provided with the placebo. For each volunteer, a blood sample from digital puncture will be obtained, and collagen-derived amino acids, di-peptides and tri-peptides will be quantified by LC-MS.; Volunteers will be provided with the collagen-derived product CP1. For each volunteer, a blood sample from digital puncture will be obtained, and collagen-derived amino acids, di-peptides and tri-peptides will be quantified by LC-MS.; Volunteers will be provided with the collagen-derived product CP2. For each volunteer, a blood sample from digital puncture will be obtained, and collagen-derived amino acids, di-peptides and tri-peptides will be quantified by LC-MS.; Volunteers will be provided with the collagen-derived product CP3. For each volunteer, a blood sample from digital puncture will be obtained, and collagen-derived amino acids, di-peptides and tri-peptides will be quantified by LC-MS.; Volunteers will be provided with the collagen-derived product CP4. For each volunteer, a blood sample from digital puncture will be obtained, and collagen-derived amino acids, di-peptides and tri-peptides will be quantified by LC-MS.
Allocation: Randomized
Intervention model: Parallel Assignment
Masking: Single (Participant)
Primary purpose: Prevention
Keywords: collagen; bioavailability; peptides; blood; oral; ingestion; randomized; blinded