Ovarian Reserve and Matrix Metalloproteinases
Condition: Oxidative Stress; Ovarian Insufficiency, Primary; Ovarian Failure, Premature
MeSH terms: Primary Ovarian Insufficiency
Study type: Observational

Summary: 
      Primary Ovarian Insufficiency (POI) is dysfunction of ovaries before 40 years of age. It has
      been reported that genetic factors, endocrine disorders, previous pelvic surgeries,
      chemotherapy and radiotherapy, and autoimmune mechanisms play a role in 10%, however the
      etiology has not been clarified in 90% of the cases. Oxidative stress and autoimmunity has
      been shown to be responsible in the etiopathogenesis of POI. Prolidase is an
      imidodipeptidase. Prolidase was shown to be related with oxidative stress and autoimmune
      diseases in previous studies. In addition, it is thought that the level of prolidase plays a
      role both in the etiopathogenesis and progression of diseases. This study aims to investigate
      the level of prolidase enzyme in women with POI.
    

Detailed description: 
      Primary Ovarian Insufficiency (POI) is dysfunction of ovaries before 40 years of age. It has
      been reported that genetic factors, endocrine disorders, previous pelvic surgeries,
      chemotherapy and radiotherapy, and autoimmune mechanisms play a role in 10%, however the
      etiology has not been clarified in 90% of the cases that are diagnosed as idiopathic POI.
      Recent studies concentrated on the role of immune mechanisms in idiopathic POI. Prolidase is
      a matrix metalloproteinase that plays a role in various metabolic processes and is
      responsible for the destruction of dipeptides containing proline and hydroxyproline. The
      prolidase has been shown to be related with oxidative stress and autoimmune diseases in
      previous studies. Prolidase was shown to be a diagnostic and prognostic value in a number of
      diseases. Proline and hydroxyproline are metabolites produced through the prolidase activity.
      Proline itself is also considered as a kind of metabolic stress indicator. On the other hand,
      free proline in the medium is known to inhibit the prolidase enzyme activity. Therefore,
      simultaneous measurement of proline and hydroxyproline levels in the medium enables to more
      accurately calculate the prolidase activity. This study aim to analyze the prolidase level in
      POI patients compared to healthy menstruating women. Besides the prolidase activity, we also
      aim to measure proline and hydroxyproline levels in order to provide the calibration for
      accurate results in terms of prolidase activity.
    

Intervention name(s): Prolidase Level; Follicle Stimulating Hormone Level; Estradiol Level; Proline Level; Hydroxyproline Level; Anti-mullerian Hormone Level; Antral Follicle Count; Prolactine Level; Thyroid Stimulating Hormone Level
Intervention type(s): Diagnostic Test; Diagnostic Test; Diagnostic Test; Diagnostic Test; Diagnostic Test; Diagnostic Test; Diagnostic Test; Diagnostic Test; Diagnostic Test
Intervention description(s): Measurement of venous blood serum level of prolidase enzyme level by ELISA method.; Measurement of venous blood serum level of follicle stimulating hormone level.; Measurement of venous blood serum level of estradiol hormone level.; Measurement of venous blood serum level of proline level.; Measurement of venous blood serum level of hydroxyproline level.; Measurement of venous blood serum level of anti-mullerian hormone level.; Ultrasonographic evaluation of antral follicle count to determine ovarian reserve.; Measurement of venous blood serum prolactine hormone level.; Measurement of venous blood serum thyroid stimulating hormone level.
Intervention MeSH terms: Hormones; Estradiol; Follicle Stimulating Hormone
Observational model: Case-Control
Time perspective: Prospective
Sampling method: Non-Probability Sample
Study population: 
        Study population includes a total of 133 participants. The study group consists of 68 women
        with the diagnosis of idiopathic POI, and the control group consists of 65 normally
        menstruating women.
      
Biospecimen description: 
      the serum samples of peripheral venous blood obtained from antecubital vein were used to
      analyze prolidase, proline and hydroxyproline levels.