Genetics of Mendelian Forms of Young Onset Alzheimer Disease
Condition: Autosomal Dominant Cases of Alzheimer 's Disease
MeSH terms: Alzheimer Disease
Study type: Observational

Summary: 
      Genetics of mendelian forms of young onset Alzheimer Disease (GMAJ project)

      Introduction : Autosomal dominant forms of Alzheimer disease (AD) are characterized by young
      age of onset. In France the prevalence was estimated at around 1000 cases. The Phenotypical
      Expression: There is diversity in phenotypical expression, associating dementia, spastic
      paraplegia, early extrapyramidal syndrome, ataxia or stroke caused by severe cerebral amyloid
      angiopathy. Diversity in neuropathological expression is demonstrated by the presence of
      cotton wool plaques, Lewy bodies with Lewy neurites and severe cerebral amyloid angiopathy.

      The study of mendelian forms is decisive in the physiopathological comprehension of AD and
      related diseases, since the alteration of those genes involved is sufficient to cause the
      disease. In AD, identification of these genetic causes allows the formulation of the amyloid
      hypothesis, which is the basis of current therapeutic developments concerning the Ab peptide.
      Currently three genes (APP, PSEN1, PSEN2) are identified in early onset mendelian forms and
      their mutations are involved in 80% of the 139 French families, studied since 1993 within the
      framework of U614 and the National Reference Centre for Alzheimer Disease in young subjects
      (CNR-MAJ).These results are arguments in favor of the GMAJ project and have two main
      objectives:

      (i) to identify new genes in mendelian AD forms without detectable alteration on known genes.

      (ii) to correlate phenotypes and genotypes within families associated with known mutations
      and extend the description of the phenotypical spectrum.

      Population: Families eligible for this study are those with at least 2 individuals with AD
      criteria (clinical or neuropathological) with onset before 65 years of age. Duration of
      inclusion is 3 years. This multicentre study groups 23 clinical centres. The inclusion number
      is 50 new families per year.

      Method: Extension and description of families with unknown genetic causes, inclusion of novel
      families with autosomal dominant AD. The successive steps of the project are the following:

      (i) Thorough phenotypical characterization in order to document family history and
      characterize the probands and affected related subjects according to standardized protocol
      for clinical, neuropsychological, biological (biomarker determination in plasma and
      cerebrospinal fluid) and imaging data (MRI, TEMP).

      (ii) Identify known genetic causes (PSEN1/2, APP) with novel autosomal dominant families.

      (iii) Extend analysis of the pedigrees of families with unknown genetic causes.

      (iv) Identify novel genetic causes within these families which are negative for known
      mutations.

      This study uses 3 methods:

        -  pangenomic search is performed for microalterations (Copy Number Variants or CNV) by
           Comparative Genomic Hybridization (CGH) with high resolution (10 kb on average).

        -  potential targets are then validated by the study of large control populations,
           intrafamilial cosegregation and functional validation.

        -  the remaining families at the end of this stage will be characterized by means of high
           throughput sequencing of all exonic areas.

           (v) Follow up study of patients and siblings with 2 goals:

        -  evaluation of the disease course and consequences (individual, familial and social);

        -  occurrence of novel AD cases within the family.

      (vi) Neuropathological description with potential novel description of atypical AD cases.

      Results and consequences: Identification of novel genetic causes of AD are of importance in
      completing the mechanisms of the amyloidergic hypothesis. This project is useful to promote
      the guidelines on the genetic diagnosis of AD in France.
    


Observational model: Case-Only
Time perspective: Prospective
Sampling method: Non-Probability Sample
Study population: 
        cases of familial Alzheimer disease with age of onset under 65
      
Biospecimen description: 
      samples with DNA
    
Keywords: Alzheimer genetics