Testicular Function After Iodine 131 Therapy in Papillary Carcinoma Patients
Condition: Thyroid Neoplasms
MeSH terms: Thyroid Neoplasms; Carcinoma, Papillary
Study type: Interventional

Summary: 
      Papillary cancer may affect patients of any age,with a good prognosis. Most of young patients
      may hence procreate as often as the general population.131iodine is an essential tool during
      treatment of thyroid cancer. According literature data, 131iodine given patients may
      experience exocrine testicular dysfunction with transient azoospermia, as a significant
      growing number of Desoxyribonucleic acid (DNA) damage may concern the patient or his
      offspring. The primary purpose will be to study a genomic instability within the germinal
      line, based on a significant mutation rate analysis in their minisatellites regions after 100
      mCi 131 iodine irradiation compared to the baseline spermogram of each patient, thus being
      his own control case in a cross-over study.The inclusion of 50 patients treated by surgery
      prior to131iodine would result in statistically significant results.This multicentric
      cross-over study with a multidisciplinary approach will act for a direct benefit on testis
      function in patients aged 18-45 treated by 131iodine for thyroid cancer. Samples measurements
      timing will be: after surgery, 2 and 12 months after 131iodine administration.
    

Detailed description: 
      Papillary cancer may affect patients of any age,with a good prognosis. Most of young patients
      may hence procreate as often as the general population.

      131iodine is an essential tool during treatment of thyroid cancer, concerning ablation of the
      orthotopic thyroid remnant and/or distant metastasis. According literature data, 131iodine
      given patients may experience exocrine testicular dysfunction with transient azoospermia, as
      a significant growing number of DNA damage may concern the patient or his offspring. No
      higher malformation risk has been described in children fathered by patients who has received
      131iodine for thyroid cancer. But animal studies showed a genomic instability leading to a
      major cancer risk in the offspring of male mice receiving isotopic radiotherapy. The deduced
      hypothesis is that a genetic toxicity of those radiation exists on the germ cells, and most
      of all that those acquired mutations may be transmitted to the next generation, majoring a
      risk of somatic mutation accumulation. So is described TCHERNOBYL exposed patients and their
      children. Unfortunately, little is known on trans-generational genomic instability.

      The primary outcome will be to study a genomic instability within the germinal line, based on
      a significant mutation rate analysis in their minisatellite regions after 100 mCi 131 iodine
      irradiation compared to the baseline spermogram of each patient, thus being his own control
      case in a cross-over study. Literature reports a risk ratio rising from 1,4 to 1,6 for a
      comparable exposition dose. Under a 2,5 % spontaneous mutation rate hypothesis and for 1000
      minisatellites studied per subject, with the mutation rate rising to 50% (RR=1,5), as the
      inter-individual variability is expected to be high : the inclusion of 50 patients treated by
      surgery prior to131iodine would result in statistically significant results.

      Secondary outcomes will be to look for deleterious endpoints on spermatogenesis, such as :
      apparition of a low number of spermatozoids, a low vitality, a low mobility, elevated
      apoptosis and aneuploidy rate found in sperm of treated subjects vs before iodine treatment.
      Therefore, the kinetics of spermogram parameters alteration will be available.
      Testosterone/LH ratio will be assessed before and after 131iodine. Direct measure of testis
      irradiation obtained by a scrotal dosimeter will permit calculation of the delivered
      irradiation dose and a dose/effect relation between the different parameters.

      This multicentric cross-over study with a multidisciplinary approach will act for a direct
      benefit on testis function in patients aged 18-45 treated by 131iodine for thyroid cancer.
      Samples measurements timing will be: after surgery, 2 and 12 months after 131iodine
      administration. Therefore, endpoints such as medical examination, exocrine and endocrine
      testis function in blood and spem sampling will be assessed in order to obtain spermogram,
      Sperm Cytogram, aneuploidy/apoptosis, minisatellites region mutation rate. Medical
      examination will take place in each local site, either from endocrinology, oncology, nuclear
      medicine departments. The sperm samples and spermogram, Sperm Cytogram, irradiation- induced
      abnormalises studies will be centralized at the CECOS (Tenon Hospital, Paris, France).
      Hormonal dosages will be processed in Bicetre Hospital (Kremlin-Bicetre, France).
      Minisatellites mutations study processed on frozen sperm samples will take place in the
      Genetic of Radiosensibility Department (Commissionership in the energy atomic, SACLAY,
      France).

      This study would permit to measure out the minisatellites region mutation rate frequency,
      aneuploidy and DNA damage frequency, and finally kinetics of those alterations after
      131iodine irradiation for human thyroid cancer. At the clinical level, practitioners would
      more precisely advise their patients on potential fertility risks and help to choose the
      appropriate practice in order to preserve it before thyroid cancer treatment. Knowing those
      results, it may redefine iodine indication. The knowledge of irradiation-induced genomic
      abnormalises is critical, regarding the growing number of examinations (Computed Tomography
      or Positron Emission Tomography-scans), known to deliver the same amount of irradiation.
    

Intervention name(s): Administration of 131 iodine
Intervention type(s): Drug
Intervention description(s): 100 mCi 131 iodine irradiation
Other intervention name(s): 131 iodine
Intervention MeSH terms: Iodine
Intervention model: Single Group Assignment
Masking: None (Open Label)
Primary purpose: Treatment
Keywords: Male; Infertility; Radioisotopes Therapy; Follicular thyroid cancer