The Effect of Toothpaste Containing Aloe Vera on Gingivitis
Condition: Generalized Gingivitis
MeSH terms: Gingivitis
Study type: Interventional
Phase: Phase 2

Summary: 
      Twenty-four adult patients (male and female) with generalized gingivitis participated in the
      double-blinded randomized crossover trial was divided into two groups, (aloe vera and Colgate
      toothpaste). a week after receiving polishing and scaling,24hours plaque re-growth, one group
      received Aloe Vera toothpaste twice times daily and the second group received Colgate
      toothpaste twice daily. The first visit included PLI measurement after that Investigator
      removed the supra gingival plaque by cotton roll to avoid contamination with the PERIOPAPER
      strip during GCF collection, then GCF collected from targeted sites(upper incisors, labial
      side)and Unstimulated saliva collected (for early colonizer bacterial load S. sanguinis and
      S. mutant) after that the other clinical periodontal parameters ( BOP) were measured and then
      scaling was done after sample collection because of gingival bleeding which occur during
      scaling then inform the patient to use the coded toothpaste which gave to the participant by
      the assistant not involve in the study so the researcher did not know the type of toothpaste
      that was given to the participant (Colgate or aloe vera twice daily).The toothpastes was
      given for one week .The codded toothpaste was gave randomly by a decision on that day for
      example on Sunday Investigator gave all the participants code 1 and on other days
      Investigator gave to other participants code 2 .In the second visit the PLI was measured
      first , then the samples were collected from the same teeth after that the other periodontal
      parameters were collected again ( BOP). After that, participants entered a 6-day wash out
      period and they were asked to resume oral hygiene measures. After the washout period, the
      same protocol was repeated for other type of toothpaste.
    

Detailed description: 
      One of the most predominant periodontal diseases is the plaque induced gingivitis. Certain
      plants used in folk medicine serve as a source of therapeutic agents that have antimicrobial
      and other multipotential effects.

      Aim: The aim of study is to evaluate the toothpaste containing high concentration of Aloe
      Vera on reduction of plaque and gingivitis and on clinical periodontal parameter (PI&BOP)
      salivary primary colonizers boat (S. sanguinis and S. mutant).and determine the effect of
      aloe vera toothpaste on the pro inflammatory cytokines (IL_1β) in the gingival crevicular
      fluid.

      Objective To evaluate the efficacy of aloe vera toothpaste on IL_1β by using ELISA technique
      and evaluate the efficacy of aloe vera toothpaste on early colonizer load using (PCR).

      Null hypothesis aloe vera has Anti-Inflammatory effect by reducing IL_1β and some clinical
      periodontal parameters (plaque index& bleeding on probing index) when used by the patients
      with regular mechanical oral hygiene.

      Alternative hypothesis the effect of aloe vera toothpaste is less than Colgate toothpaste on
      the inflammatory marker IL_1β, early load and some clinical parameters (PI and BOP).

      Materials and methods Twenty-four adult patients (male and female) with generalized
      gingivitis participated in the double-blinded randomized crossover trial was divided into two
      groups, (aloe vera and Colgate toothpaste). A week after receiving polishing and
      scaling,24hours plaque re-growth. In zero day, after 24h and 7 days' time points, plaque
      index and bleeding on probe was recorded, and then the participants entered a 7-day washout
      period with regular oral hygiene measures. And 7days to used other type of toothpaste (aloe
      vera or Colgate).in the first visit (zero day) GCF collected from targeted sites (upper
      incisors, labial side). In the second visit (after 24 hours) saliva collected (for early
      colonizer load) and the patient brushing teeth and wait 2 hours, after 2hour saliva collected
      again. the GCF collected in third visit (after7 days), fourth visit (after wash out period)
      and fifth visit from the same teeth. Saliva collected in third and fifth visit.
    

Intervention name(s): aloe vera toothpaste; Colgate Total
Intervention type(s): Drug; Drug
Intervention description(s): aloe vera toothpaste have antimicrobial and other multipotential effects.; Whitening toothpaste that removes and prevents new stains Fights bacteria on teeth, tongue, cheeks and gums for whole mouth healthy Mint toothpaste for long lasting fresh breath Helps prevent plaque, tartar, cavities & gingivitis
Other intervention name(s): colgate SF
Intervention MeSH terms: Listerine; Sodium Fluoride
Allocation: Randomized
Intervention model: Crossover Assignment
Intervention model description: twenty-four adult patients with generalized gingivitis participated in the double blinded randomized controlled crossover study divided into two groups, aloe vera and colgate toothpaste used during seven days , one group received aloe vera toothpaste twice times daily and the second group received colgate toothpaste twice dialy .
Masking: Double (Participant, Investigator)
Primary purpose: Health Services Research