Bioavailability and Bioactivity of Mango Polyphenols.
Condition: Nutrition, Healthy
Study type: Interventional

Summary: 
      To date however, the majority of information on bioaccessibility and bioavailability of mango
      phytochemicals has been generated in vitro and animal models using isolated compounds or
      extracts from mango leaf and mango seed kernels, which do not represent the
      delivery/absorption of phytochemicals from a complex food matrix such as mango puree.
      Consequently, a paucity of data exists on the bioavailability and metabolism of
      (poly)phenolic compounds following ingestion of fresh mango puree either using targeted or
      untargeted metabolomics approaches. Mango puree (poly)phenolic bioavailability in humans
      (both healthy and ilesotomists) will be investigated using both targeted and untargeted
      metabolomics; further we will establish the bioactivity of mango (poly)phenols with respect
      to gastrointestinal health. A comprehensive understanding of the bioavailability of fresh
      mango puree (polyp)henols, will have direct relevance to the development of any mango-based
      novel food products.

      Acute bioavailability feeding study in two groups Ileostomists (n= 10) and health adults
      (N=10), as described below. Prior to attending the visit participants were asked to follow a
      restriction diet for 48 hours before the study and also during the 24 hours of the study
      clinic visit, which involved the intake of food containing low levels of polyphenols.

      Ileostomists (n=10) Twenty four hours before clinic visit, the participant started a urine
      collection, and were asked to fast from 9pm the night before the visit (i.e. no food taken
      overnight and no breakfast). On the morning of the study day, the participants were asked to
      attend the clinic, bringing their overnight stoma bag. The 24 hr urine sample was provided,
      and the overnight stoma bag was removed and replaced with a new stoma bag by the participant,
      and passed to the researcher. A cannula was fitted in the participants arm by a qualified
      phlebotomist and a blood sample (~14 ml, 6 ml draw off + 8ml sample) collected. The
      participant was given ~300 g of mango purée to consume. Blood samples (~14 ml, 6 ml draw off
      + 8ml sample) were collected at hourly intervals for 8 hrs from the cannula (0,
      0.5,1,1.5,2,3,4,6,8 and 24 hrs). Urine was collected between 0-4 hrs, 4-8hrs and 8-24 hrs, a
      fresh sample bottle was provided at each time point. Ileal samples were collected at 0-4 hrs,
      4-8hrs and 8-24 hrs, the stoma bag was removed and replaced with a new stoma bag by the
      participant at each sampling point. A restriction diet lunch was provided to the participant
      after the 4 hr blood draw. After the 8 hr sample the participant was free to return home. The
      next morning the participant returned to the clinic and the 24 hr samples (blood, urine,
      ileal) collected. The urine & ileal samples were collected, and normal a normal phlebotomy
      draw will be used to collect the 24 hr blood sample. The participant is then finished the
      study was free to return to their normal diet.

      Ileostomist participants each provided 4 ileal fluid samples, 4 urine samples and 10 blood
      samples over the sampling period.

      Healthy participants (n=10) Twenty four hours before clinic visit, the participant started a
      urine collection, they were asked to fast from 9pm the night before the visit (i.e. no food
      taken overnight and no breakfast). On the morning of the study day, the participant was asked
      to attend the clinic. The 24 hr urine sample was collected and a faecal sample was provided.
      A cannula was fitted in the participants arm by a qualified phlebotomist and a blood sample
      (~14 ml, 6 ml draw off + 8ml sample) collected. The participant was then given ~300 g of
      mango purée to consume. Blood samples (~14 ml, 6 ml draw off + 8ml sample) were collected at
      hourly intervals for 8 hrs from the cannula (0, 0.5,1,1.5,2,3,4,6,8 and 24 hrs). Urine
      samples were collected between 0-4 hrs, 4-8hrs and 8-24 hrs, a fresh sample bottle was
      provided at each time point. A restriction diet lunch was provided to the participant after
      the 4 hr blood draw. After the 8 hr sample the participant was free to return home. The next
      morning the participant returned to the clinic and the 24 hr samples (blood, urine, faecal)
      collected. The urine and faecal samples were collected, and normal a normal phlebotomy draw
      was used to collect the 24 hr blood sample. The participant then finished the study and was
      free to return to their normal diet.

      Healthy adult participants each provided in total 2 faecal samples, 4 urine samples and 10
      blood samples over the sampling period.
    


Intervention name(s): Mango puree
Intervention type(s): Dietary Supplement
Intervention description(s): Mango puree 300g
Intervention model: Parallel Assignment
Intervention model description: Acute intervention in populations with and without colon
Masking: None (Open Label)
Primary purpose: Prevention
Keywords: Mango; Polyphenols; Ileostomy; GI tract