Effect of Ozone Therapy on Aerobic Exercise Performance
Condition: Ozone Therapy; Exercise Performance
Study type: Interventional

Summary: 
      The goal of this clinical trial is to investigate the effect of the ozone therapy applied
      using the major autohemotherapy method on exercise performance in male healthy individuals.

      The main question's aims to answer are:

        -  Is ozone therapy applied with the major autohemotherapy method increase the performance?

        -  Is ozone therapy applied with the major autohemotherapy method improve the recovery
           after exercise?

        -  Participants are asked to continue the same exercise program throughout the study. They
           were given 10 sessions of ozone therapy over 6 weeks.

      Researchers compared the before and after treatment performance and recovery data.
    

Detailed description: 
      Medical ozone is a mixture of pure ozone gas (0.05-5%) and pure oxygen (99.95-95%). It is
      produced from pure medical oxygen by medical ozone generators. It is suggested that ozone
      therapy, which is used to treat a wide variety of diseases and is especially useful in the
      treatment of tissue ischemia and cardiovascular disorders, creates a mild, temporary and
      controlled oxidative stress and modulates the antioxidant system and immune system. It was
      reported that ozone exerted its effects by induction of reactive oxygen species and lipid
      oxygen products known as ozonides. The therapy modulates the immune system by balancing
      inflammatory and anti-inflammatory cytokines, increasing antioxidant enzymes and increasing
      glutathione production. In addition, it increases the production of 2,3-diphosphoglycerate
      (2,3-DPG) by causing an increase in the rate of red blood cell (RBC) glycolysis, improves red
      cell rheology, increases the oxidative carboxylation of pyruvate, and activates the hexose
      monophosphate pathway by stimulating adenosine triphosphate (ATP) production. With a decrease
      in potential of hydrogen (pH) and a slight increase in 2,3-DPG, the sigmoid curve of
      oxyhemoglobin shifts to the right due to the Bohr effect. The shift to the right reduces the
      chemical binding of oxygen to hemoglobin, facilitating oxygen delivery to ischemic tissues.
      Considering that the most important determinant of exercise performance is tissue oxygenation
      and that the antioxidant system has a positive effect on fatigue, these data lead to the idea
      that ozone can improve exercise performance.

      There are a few studies in the literature investigating the effect of major ozone treatment
      on exercise performance. The arguments suggesting that major ozone therapy is effective in
      exercise performance are based on publications made outside of this subject, which include
      the mechanism of action of major ozone therapy.

      Thus, it was aimed to evaluate the effects of the ozone therapy applied using the major
      autohemotherapy method on exercise performance with changes in fatigue, exercise duration,
      blood lactate changes level, anaerobic threshold, and maximal oxygen consumption (VO2max).
    

Intervention name(s): ozone therapy
Intervention type(s): Other
Intervention description(s): Medical ozone is a mixture of pure ozone gas (0.05-5%) and pure oxygen (99.95-95%). It is produced from pure medical oxygen by medical ozone generators. It is applied to the participants 10 sessions by the intravenous method.
Intervention model: Single Group Assignment
Masking: None (Open Label)
Primary purpose: Other