The Research of Novel Electrolyzed Water Spray to Eradicate Bacteria E-coli and an Attenuated Human Flu Virus
Condition: Bacterial Growth; Novel Influenza A (H1N1)
MeSH terms: Influenza, Human
Study type: Interventional

Summary: 
      The purpose of this study is to test whether spraying the hand of patients by use of the
      novel electrolyzed water spray will decrease the number of bacteria E-coli and the virus
      titer of an attenuated human flu virus.
    

Detailed description: 
      In this study, water including tap water, pure water and salt water, and an apparatus for
      producing electrolyzed water were used to generate an electrolyzed water mist spray or spray.
      This instantly generated electrolyzed water mist spray or spray has oxidation-reduction
      potential（ORP）≥1200mv,and contains non-specific total oxidation capacity which equals to
      0.28±0.10ppm,0.06±0.04ppm and 3.92±0.39ppm of dissolved ozone. This instantly generated
      electrolyzed water mist spray or spray does not release detectable ≥0.1mg/m3 of gaseous
      ozone. This instantly generated electrolyzed water mist spray or spray has pH 8.4±0.4 and
      releases negative air ion. The apparatus for producing electrolyzed water has a positive
      electrode which is covered by a conductive diamond material (Patent# CN215308550U).

      In this study, we hypothesize that the instantly generated electrolyzed water mist spray or
      spray relieves skin and mucosal inflammation including the itches through indirect actions by
      killing microbials and reducing endotoxin level and direct action by serving as an
      inflammatory inhibitor.

      Detection of residual E. coli in the collected water samples. 1 ml of the collected water
      sample is inoculated on the surface of the solid LB agar plates and cultured overnight at
      37°C in an incubator. Calculate the total number of bacterial colonies as CFU. In other
      words, the total number of bacteria/ml inoculated water sample (CFU) is the number of
      colonies formed on the surface of the plate. It is assumed that the bacteria on the hands are
      mostly washed out into the drain water sample pool and that the left over bacteria on the
      hand after rinsing is negligible. The leftover also can be measured by pressing the rinsed
      hand after on a large LB agar plate and then count the colonies (unstained and stained) after
      overnight incubation at 37°C.

      Detection of residual human influenza virus H1N1 titers in the collected water samples. Viral
      titer (TCID50) is used to measure the infection of the dosage of 50% MDCK cells. MDCK cells
      were inoculated into 96-well plates at 1.0×104 cells per well and cultured overnight at 37°C
      in a cell culture incubator. We wash the hands and collect the drained water for the titer
      measurement. 100 ul of the water sample after the multiple proportion dilutions is inoculated
      into 96-well plates which contain overnight cultured MDCK cells. After 3 days of incubation
      at 37°C, TCID50 was calculated using the Reed and Muench method. 1 plaque forming unit (PFU)
      is equal to 1 alive virus particle.
    

Intervention name(s): A novel electrolyzed water spray reduces numbers of bacteria E-coli and an attenuated human flu virus on human hands
Intervention type(s): Device
Intervention description(s): Electrolyzed water spray group will use the novel electrolyzed water spray to flush the hands that daub E. coli and attenuated human influenza virus H1N1 for 6 seconds per hand, and then use 4 seconds to collect a sample of the water flowing from the sprayed hand and apply it to the plate. Untreated tap water group will use the untreated tap water spray to flush the hands that daub E. coli and attenuated human influenza virus H1N1 for 6 seconds per hand, and then use 4 seconds to collect a sample of the water flowing from the sprayed hand and apply it to the plate.
Allocation: Non-Randomized
Intervention model: Parallel Assignment
Masking: None (Open Label)
Primary purpose: Treatment
Keywords: Electrolyzed water device; Hydroxyl radical; Tap water; Spray; E-coli; Human flu virus