Plasma-Induced Changes in the Metabolome Following Polyphenol Consumption
Condition: Processes, Metabolic
Study type: Interventional

Summary: 
      The goal of this clinical trial is to compare the changes in the human metabolome following a
      low or high acute dose of European tart cherries. The main question it aims to answer is:

      what effect do European tart cherries have on the human metabolome?

      Participants will attend the laboratory at the university on 3 separate occasions. Take a 3
      different supplements: a placebo, low or high dose of European tart cherry supplement. Have
      venous blood samples taken as baseline, then 1, 2, 3, 5 and 8 h post ingestion of the
      supplement. Researchers will compare placebo, low and high to see if changes the human
      metabolome.
    

Detailed description: 
      Participants Twelve healthy participants (nine male and three female) were recruited for this
      study; the mean ± SD age, stature, mass and body mass index (BMI) were 29 ± 7 years old,
      174.8 ± 9.3 cm, 77.29 ± 10.52 kg and 25.23 ± 1.87 kg/m2, respectively. All participants were
      healthy, as assessed by a health-screening questionnaire and provided written, informed
      consent. Exclusion criteria for the study were as follows: food allergy (as discussed with
      research team), history of gastrointestinal, renal, cardiovascular or thyroid disease and
      current use of any food supplementations. This study received ethical approval from
      Northumbria University Ethics Committee (reference number: 39904) (clinical trial tbc).

      Experimental Design The study utilised a double blind, three-arm cross-over,
      pseudo-randomised (counter-balanced to eliminate order effects) design in order to identify
      metabolomic shifts following the acute ingestion of a low dose (LOW), high dose (HI) of TC or
      placebo (CON). A washout period of at least 7 days (but not greater than 21 days) between
      each phase was implemented. Participants were required to attend the start of each phase of
      the study at 7:45 am following a 10 h overnight fast in order to account for diurnal
      variation (Figure 1). Upon arrival to the lab, the participants baseline blood pressure (BP)
      was recorded in a rested state (following at least 5 mins of sitting), then cannulated for
      serial blood sampling. A standardized breakfast was then provided. Subsequent measures were
      then taken at baseline, 1, 2, 3, 5 and 8 hrs post-VTC consumption. Water was provided
      ad-libitum, but no additional food was given during testing visits.

      Treatments and Dietary Control The low (LOW) and high (HI) dose consisted of 3 or 6 g of the
      TC supplement (CherryCraft), respectively, or a 3g capsule placebo (CON) matched for caloric
      content using maltodextrin. The low TC dose provided approximately 60-78 mg of anthocyanins,
      8.07 kcal and had a macronutrient content of 1.215 g, 0.003 g, 0.042 g, for carbohydrates,
      fat and protein, respectively. The high TC dose provided approximately 120-156 mg of
      anthocyanins, 16.14 kcal with 2.43 g, 0.006 g, 0.08 g for carbohydrates, fat and protein,
      respectively.

      Participants were requested to follow their habitual diet throughout the trial. Food diaries
      were completed for the 24 hrs before each testing visit to replicate (as close to) prior to
      testing on following testing visits. The standardized breakfast consisted of white toast (2
      slices of Sainsbury's medium white bread) and butter (approx. 16g of Bertolli/Vitalite (a
      vegan alternative, matched as closely as possible for calories and macronutrient content)).
      Participants were given water to drink ad-libitum throughout the testing visits, this was
      measured and refilled from baseline, and 1, 2, 3, 5 and 8 hrs post breakfast and supplement
      ingestion.

      Blood Pressure Monitoring Blood pressure was monitored throughout the testing visits,
      immediately upon arrival to the lab and, 1, 2, 3, 5 and 8 hrs following ingestion of the
      supplement (Keane, George, et al., 2016).

      Blood Sampling Procedure Venous blood samples (~10 mL) were collected from the antecubital
      fossa into lithium heparin (10 mL) tubes, immediately upon arrival to the lab and 1, 2, 3, 5
      and 8 hrs following ingestion of the supplement. Samples were immediately centrifuged (3000 ×
      g) at 4°C for 20 min, plasma was then aspirated and pipetted into ~1 mL aliquots and then
      immediately stored at -80 °C for later analysis.

      Untargeted Plasma Analysis The sample was prepared by firstly being thawed on ice, then 200
      µL of plasma was extracted and vortexed with 1000 µL of analytical grade methanol, then
      sonicated in a water ice bath for 15 mins. The samples were then centrifuged for 15 min at
      15,000 rpm at 4°C, before the supernatant was dried in a vacuum pre-concentrator to dryness
      for 3 h. The residue was reconstituted in 100 µL of analytical grade water (vortex for 30 s
      and sonicated for 15 mins), before being filtered via 0.22 micron Costar spin filter and put
      in 1.5 mL autosampler vial with a 200 µL microinsert.

      Metabolite characterizations were performed on a LCMS (Vanquish Liquid chromatography Front
      end connected to IDX High Resolution Mass Spectrometer system, Thermo Scientific, Hemel
      Hempstead, United Kingdom). The MS data were acquired using the AcquieX acquisition workflow
      (data dependent analysis). The MS operating parameters were as follows: MS1 mass resolution
      60K, for MS2 30K stepped energy (HCD) 20,25,50 scan range 100-1000, RF len (%) 35, AGC gain,
      intensity threshold 2e4 25% custom injection mode with an injection time of 54 ms. An
      extraction blank was used to create a background exclusion list and a pooled QC were used to
      create the inclusion list. C18 RP, the chromatographic separation were archived using a
      Waters Acquity UPLC T3 HSS column (2.1 x 150mm with particle size of 1.7 μm), operating at
      45°C with a flow rate of 200 μL/min. The LC gradient consist of binary buffer system, buffer
      A (LC/MS grade water/ACN 95/5 v/v) and Buffer B (LC/MS grade ACN/Water 95/5 v/v). Independent
      buffers system was used for positive and negative mode acquisition, the pH of buffers were
      adjusted using 0.1% formic acid for both. The LC gradient was the same for both polarities,
      5% B at T0 hold for 1.5 min and linearly decrease to 95%B at 11min hold for 4 mins and return
      to starting condition and hold for further 4.5min (column stabilization). The voltage applied
      for positive mode was 3.5 kV and injection volume was 3 μL. The HESI condition were as
      follows for 200 μL: sheath gas: 35, aux gas 7 and sweep gas of 0. Ion transfer tube
      temperature was 300°C and vaporizer temperature was 275°C.

      Data Analysis Statistical analysis was performed using SPSS (SPSS, Inc., Chicago, IL.).
      Descriptive statistics are reported as mean ± SD. BP was analysed using a treatment (low vs.
      high dose vs. placebo) by time (baseline, 1, 2, 3, 5 and 8 hrs post-supplement) repeated
      measures analysis of variance (RMANOVA). Mauchly's test of sphericity was used to check
      homogeneity of variance for all variables; where necessary, any violations of the assumption
      were corrected using the Greenhouse-Geisser adjustment. Significant interaction effects were
      followed up using post hoc analysis. The alpha level for statistical significance was set at
      0.05.
    

Intervention name(s): Tart Cherry; Placebo
Intervention type(s): Dietary Supplement; Dietary Supplement
Intervention description(s): Tart cherry supplementation in two doses; Control group
Other intervention name(s): European
Allocation: Randomized
Intervention model: Crossover Assignment
Masking: Triple (Participant, Investigator, Outcomes Assessor)
Primary purpose: Basic Science