To Study the Healing Effect of 0.25% Lemongrass Oil Mouthwash on Chronic Periodontitis Patients.
Condition: Chronic Periodontitis
MeSH terms: Periodontitis; Chronic Periodontitis
Study type: Observational

Summary: 
      Periodontitis is the inflammation of the tissues supporting the teeth. Lemongrass (Cymbopogon
      Citratus) oil mouthwash can be used with subgingival scaling in the treatment of periodontal
      diseases. Transforming growth factor beta (TGF-β) is a multifunctional cytokine belonging to
      transforming growth factor superfamily and its elevated levels are detectable in inflammatory
      and healing process The present project will be conducted to evaluate the expression of TGF-β
      levels in chronic periodontitis patients after ultrasonic scaling and using 0.25% lemongrass
      oil mouthwash. TGF-β is a salivary marker of periodontitis.
    

Detailed description: 
      Periodontitis is the inflammation of the tissues supporting the teeth which include cementum,
      gingiva, periodontal ligaments and alveolar bone. Periodontitis typically follows untreated
      gingivitis, a shift in the composition of biofilm from gram positive cocci to dominantly gram
      negative actinomyces and infection by proteolytic obligate anaerobes resulting in progressive
      destruction of periodontal ligament, formation of pocket around the teeth and resorption of
      alveolar bone with ultimately loosening or loss of teeth. The present project will be
      associated with very common forms of periodontitis (in which the inflammation results in
      periodontal tissue destruction and alveolar bone resorption) and gingivitis (in which the
      inflammation is confined to gingivae and is reversible with good oral hygiene). Lemongrass
      (Cymbopogon Citratus) oil mouthwash can be used with subgingival scaling to eliminate the
      pathogens of periodontitis and to treat the inflammation and the damage done to periodontium
      by it. It possess anti-oxidant, anti-inflammatory, anti-microbial, anti-fungal and astringent
      properties. Citral epoxide which is an ingredient of lemongrass extract is an active
      anti-inflammatory and anti-bacterial agent. It is useful in dentistry and has a wide role in
      the treatment of periodontal diseases. Transforming growth factor beta (TGF-β) is a
      multifunctional cytokine belonging to transforming growth factor superfamily and its elevated
      levels are detectable in inflammatory and healing process The present project will be
      conducted to evaluate the expression of TGF-β levels in chronic periodontitis patients after
      ultrasonic scaling and using 0.25% lemongrass oil mouthwash. TGF-β is a salivary marker of
      periodontitis. A sample size of 30 patients either male or female aged between 40 and 60
      years with chronic localized or generalized periodontitis with pocket depth of >3mm affecting
      at least two nonadjacent sites/teeth will be selected. This sample will be divided into two
      equal groups. In the experimental 1 group, only sub-gingival scaling will be performed. In
      the experimental 2 group, sub-gingival scaling will be performed, followed by use of 0.25%
      lemongrass oil mouthwash twice daily for 14 days. Saliva sample will be collected on days 0,
      7 and 14 and level of salivary biomarker, TGF-β will be measured with the ELISA technique.
      The data will be entered and analyzed using SPSS 22. Mean ±SD will be given for quantitative
      variables like TGF-β level. A p value of ≤ 0.05 will be considered as statistically
      significant.
    

Intervention name(s): 0.25% lemon grass oil mouthwash
Intervention type(s): Drug
Intervention description(s): 0.25% lemon grass oil mouth wash given twice daily for fourteen days to the patients of chronic periodontitis after scaling in experimental group and in control group only scaling was done.
Other intervention name(s): lemon grass mouth wash
Observational model: Case-Control
Time perspective: Prospective
Sampling method: Probability Sample
Study population: 
        Male or female with age range between 40-60 years with generalized periodontitis were taken
        for the study