Endometrial Tissues and Mononuclear Cells Receptivity in Pathogenesis of Endometrial Proliferative Processes
Condition: Endometrial Hyperplasia Without Atypia; Endometrial Hyperplasia With Atypia; Endometrial Cancer; Peripheral Blood Mononuclear Cells; Postmenopause; Estrogen Receptor; Progesterone Receptor
MeSH terms: Endometrial Neoplasms; Endometrial Hyperplasia; Hyperplasia
Study type: Observational

Summary: 
      A prospective observational study of endometrial tissue and peripheral blood mononuclear
      cells receptivity to sex steroid hormones in postmenopausal patients with endometrial
      proliferative processes
    

Detailed description: 
      Endometrial cancer is in third place among cancer diseases in female population of Russia.
      The peak morbidity occurs during the postmenopausal period. In this regard, early diagnosis
      of previous endometrial proliferative processes and effective methods for their treatment are
      relevant. However, failures with hormonal therapy are often observed. This may be due to the
      low receptivity of the pathological tissue. It is also known that the functional activity of
      immunocompetent cells is controlled by the immune system, however, studies of the receptivity
      of peripheral blood mononuclear cells to sex steroid hormones were carried out in healthy
      blood donors. Changes in mononuclear cells receptivity may be one of the pathogenetic links
      in the development of endometrial proliferative processes and endometrial cancer. This may
      also influence the effectiveness of their treatment. In this regard, the purpose of the study
      is to evaluate the role of the expression of estradiol and progesterone receptor genes in
      endometrial tissue and peripheral blood mononuclear cells in the occurrence of endometrial
      proliferative processes in postmenopausal patients with a pathogenetic justification for the
      choice of treatment method. To achieve this goal, the investigators investigated the
      expression level of estrogen and progesterone receptors (ERa, ERb, GPER, PRA, PRB, mPR,
      PGRmC1) by RT-PCR in pathological endometrial tissue and peripheral blood mononuclear cells.
      GABDH was used as a comparison gene. The data obtained made it possible to determine the
      significance of mononuclear cell receptivity in the pathogenesis of endometrial proliferative
      processes.
    

Intervention name(s): endometrial tissue and peripheral blood mononuclear cells receptivity
Intervention type(s): Diagnostic Test
Intervention description(s): Participants investigated the expression level of estrogen and progesterone receptors (ERα, ERβ, GPER, PRA, PRB, mPR, PGRmC1) by RT-PCR in pathological endometrial tissue and peripheral blood mononuclear cells. GABDH was used as a comparison gene.
Observational model: Case-Control
Time perspective: Prospective
Sampling method: Non-Probability Sample
Study population: 
        The study included 92 patients with endometrial proliferative processes: endometrial polyp
        - 37, endometrial hyperplasia without atypia - 7, atypical endometrial hyperplasia - 8,
        endometrial cancer - 40