Vitamin D Effect on A Disintegrin-like And Metalloprotease Thrombospondin1 Motif 13& Interleukin 6 in Leukemia
Condition: Acute Myeloid Leukemia; Vitamin D Deficiency; ADAMTS13; Interleukin 6
MeSH terms: Leukemia; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Vitamin D Deficiency
Study type: Interventional
Phase: Phase 1/Phase 2

Summary: 
      A Disintegrin-like And Metalloprotease with Thrombospondin type 1 motif 13 (ADAMTS13)
      deficiency was incriminated in poor prognosis, high probability of serious complications and
      mortality in acute myeloid leukemia (AML) patients. Interleukin 6 (IL-6) produced from AML
      blasts decreases Cluster of differentiation 34 positive(CD34+) cells differentiation, and
      inhibits the ADAMTS13 actions. Vitamin D "as an Immune-modulator" inhibits the
      pro-inflammatory cytokines including IL-6. So, supplementation of vitamin D might help down
      regulation of interleukin-6 production.

      Aim of the study To evaluate the potential relation between Vitamin D status, ADAMTS13 and
      IL-6 in AML patients.

      Objectives

        1. Assess Vitamin D level in AML patients

        2. Assess ADAMTS13 and IL-6 in AML patients

        3. Correlate between Vitamin D level and both of ADAMTS13 and IL-6
    

Detailed description: 
      I. Setting The study will be carried out in wards of ZAGAZIG University Hospitals II.
      Subjects The study will be conducted on patients with de novo diagnosis with acute myeloid
      leukemia III. Inclusion criteria

        1. Patient's consent to share in the study

        2. Patients' age >18 years

        3. Patients with de novo acute myeloid leukemia IV. Exclusion criteria

      1. Patients refusing to share in the study 2. Non-Egyptian patients 3. Patients' age < 18
      years 4. Pregnant women 5. Patient's with other malignancies 6. Patients with known
      congenital thrombotic/ hemorrhagic diseases 7. Patients with Thrombotic Thrombocytopenic
      Purpura 8. Patients with auto-immune diseases

      V. Study Design This is a Non-randomized control trial I. Sample size up Finding that
      ADAMTS13 level in AML patients before treatment is 455+_120 ng/ml versus 570+_100 ng/ml after
      treatment, the sample was calculated to be 30 patients, 15 patients in each group by using
      (open EPI) at confidence level 95 and power 80 I. Activities Patients attending to ZAGAZIG
      University Hospitals. Upon agreement, every patient will be asked to provide written informed
      consent according to the Declaration of Helsinki of 1979.

      All patients will be subjected to the following:

        1. Full History taking through an interview

        2. Clinical examination

        3. Laboratory investigations including:

             1. Complete blood picture ( CBC) using ( Sysmex XS 500)

             2. Peripheral blood film examination

             3. Erythrocyte sedimentation rate ( Westergren tubes )

             4. Prothrombin time, Partial thromboplastin time (Sysmex CS)

             5. C-reactive protein , Liver function tests & Kidney function tests (Roche
                Diagnostics, Cobas 8000 c702, Switzerland)

             6. Hepatitis C virus antibody, Hepatitis B virus surface antigen and Human deficiency
                virus antibody

             7. Diagnosis of Acute myeloid leukemia according to clinical findings, CBC, peripheral
                blood film examination, Bone marrow aspiration, Immunophenotyping (a FACSCAN,
                Becton Dickinson, San Jose, California, USA)and Cytogenetic analysis.

        4. Measuring IL-6 and ADAMTS13 using (Luminex Corporation, Luminex® 200 trademark , Austin,
           USA) before and after induction therapy

        5. Measuring Vitamin D serum level using (Roche Diagnostics, Cobas 6000 e 601, Switzerland)
           before and after induction therapy and vitamin D supplementation based on its
           deficiency.

        6. Radiology:

             -  Echocardiogram

             -  Abdominal ultrasound ( If needed)

             -  Chest X-Ray II. Data collection Demographic data of the patients will be recorded
                including name, age, sex and residence. In addition, careful history taking and
                clinical examination will be done, and data will be registered in special form III.
                Statistical analysis Our study will be carried on 30 patients with de novo AML.
                Subjects will be divided into (2) groups as regard treatment of Vitamin D
                deficiency at the onset of AML diagnosis. Vitamin D therapy will be given to 15
                deficient subjects for one month with the recommended doses. All the analytes will
                be assayed before and after the induction chemotherapy course. Both of these groups
                will be compared statistically using Statistics program smart solution 22 (SPSS22).

      Administrative design:

      Approval will be asked from ZAGAZIG University Institutional Review Board (IRB).

      C. Ethical considerations

        1. The study group will be informed about the nature and the purpose of the study and
           informed consent will be taken.

        2. The study group will not be exposed to any harm or risk.

        3. Patient's data will be confidential.
    

Intervention name(s): Cholecalciferol 2800 I.U. ml oral drops
Intervention type(s): Drug
Intervention description(s): (Cholecalciferol 2800 I.U. ml) Oral Drops 15 ml. supplied with a dropper.
Composition:
Each 1 ml (= 28 drops) of oral solution contains: Vitamin D3 (Cholecalciferol) 2800 I.U., (each drop contains 100 IU of vitamin D3).
Intervention MeSH terms: Cholecalciferol; Vitamin D
Allocation: Non-Randomized
Intervention model: Parallel Assignment
Masking: None (Open Label)
Primary purpose: Treatment
Keywords: ADAMTS13; Interleukin 6; Vitamin D; Acute Myeloid Leukemia