Yeast Cells as Antioxidant-Producing Probiotics
Condition: Infertility, Male
MeSH terms: Infertility; Infertility, Male
Study type: Interventional
Phase: Phase 1

Summary: 
      The purpose of this study is to evaluate the efficacy of Baker's yeast extractions ,
      probiotic antioxidant, in scavenging the oxidative stress status mediated damage on sperm
      motility,progressive motility and vitality as well as comparing this potency to that of
      Vitamin E in infertile men.
    

Detailed description: 
      The practical part of the study took place from August 2020 to November 2021. The Study
      population was made up of infertile males attending Hawaa fertility center, banha city,
      Qaliubiya, Egypt.

      40 study samples were collected from patients who indicated willingness to participate in the
      study and have had 3 to 7 days of sexual abstinence, using the masturbation method and
      ejaculated in wide mouthed plastic container as described by World Health Organization (WHO).

      Samples were allowed to liquefy for 20 minutes and were examined for volume, viscosity,
      spermatozoa count by Neubauer haemocytometer , oxidative stress level measurement by
      Oxisperm, percentage of progressive motility and non-progressive motility and sperm vitality.

      The Semen specimens were divided into ten equal fractions. First fraction was control, 0.1ml
      of liquefied semen mixed with 0.1ml Ham's F10 medium and incubated at 37o C for 30 minutes.,
      2nd: Vit. E, 3rd (Yeast extraction ( YE).Vitamin C was obtained from (Sigma, Germany), 3rd,
      4th, 5th fractions (Vit. C. 1,2,3), 0.1ml liquefied semen was mixed with 0.1ml Ham's F10
      medium supplemented with (0.02, 0.04, 0.06 mg/ml) Vitamin C respectively and incubated at 37o
      C for 30 minutes.

      After semen specimen treated in all fractions, they were examined and assessed for
      microscopical changes, total motility, progressive motility, vitality, level of oxidative
      stress (OS) were detected in all fractions 30 minutes later after addition of the
      antioxidants.
    

Intervention name(s): Vit E; Yeast; Vit C 1; Vit C 2; Vit C 3
Intervention type(s): Dietary Supplement; Dietary Supplement; Dietary Supplement; Dietary Supplement; Dietary Supplement
Intervention description(s): 0.1ml liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (2 mg/ml) Vitamin E and incubated at 37o C for 30 minutes.; 0.1ml liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (20 mg/ml) yeast extraction and incubated at 37o C for 30 minutes.; 0.1ml liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (0.02 mg/ml) Vitamin C and incubated at 37o C for 30 minutes.; 0.1ml liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (0.04 mg/ml) Vitamin C and incubated at 37o C for 30 minutes.; 0.1ml liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (0.06 mg/ml) Vitamin C and incubated at 37o C for 30 minutes.
Intervention MeSH terms: Ascorbic Acid
Allocation: Non-Randomized
Intervention model: Parallel Assignment
Intervention model description: control, vit E, vit C, Yeast
Masking: Double (Participant, Outcomes Assessor)
Primary purpose: Basic Science
Keywords: Male infertility; oxidative stress; antioxidants; probiotics; Vitamin E; Baker's Yeast