Clinical Analysis of Vitamin B6 in Sepsis
Condition: Sepsis; Vitamin B6; Kidney Injury; Inflammatory Response; Oxidative Stress Response
MeSH terms: Sepsis; Toxemia
Study type: Interventional

Summary: 
      Methodology Patients A total of 128 patients with sepsis and AKI who were admitted to several
      centers including Huzhou first people's Hospital combined with Wuxing People's Hospital,
      Linghu people's Hospital and Nanxun people's Hospital from November 1, 2021 to October 31,
      2022 were included in the study. And all patients were diagnosed by clinical examination,
      Diagnostic criteria sepsis was diagnosed according to the international Sepsis-3 for patients
      with suspected infection using the quickly Sepsis related organ failure assessment (qSOFA).
      The qSOFA score consists of only three criteria: Glasgow Coma Scale (GCS) <15, systolic blood
      pressure ≤ 100 mmHg, and respiratory rate ≥22/min. A qSOFA score of 2 or more points
      indicates suspected sepsis. Criteria for AKI was defined according to the Kidney Disease
      Improving Global Outcomes (KDIGO), by the presence of one of the following: ①Increase in SCr
      by ≥ 0.3mg/dl (≥26.5 μ mol/L) within 48h；② Renal impairment is known or increase in SCr by
      ≥50% within 7days; ③ Oliguria for ≥4 hours.

      All patients were authorized by their families to sign informed consent, and the study was
      approved by the ethics committee of the hospital.

      Inclusion criteria: ①18-65 years old; ② The hospital survival time was more than 48 hours,
      and the medical records were complete; ③There is no history of vitamin B6 use in the recent
      period of admission (within 2 weeks before admission).

      Exclusion criteria: ①Patients with chronic renal insufficiency or renal failure in the past;
      ②Related renal injury caused by reasons other than sepsis; ③At the time of admission to ICU,
      there was cardiac failure or cardiogenic shock in combination with sepsis; ④Patients who use
      nephrotoxic drugs or contrast agents; ⑤Previous kidney transplantation; ⑥Patients with
      restrictive use of positive inotropic drugs (such as left ventricular outflow tract
      stenosis); ⑦Age<18 or>65; ⑧pregnant woman.

      Treatment 128 patients were divided into experimental and control group by random number
      table method, 64 patients in each group. Both groups were given routine treatment of sepsis
      and corresponding treatment of primary disease. The Patients in experimental group were given
      vitamin B6 injection 300mg/d (100mg/2ml× 3) intravenous injection, the course of treatment is
      one week or until the patient dies. That in control group were injected with 0.9% sodium
      chloride solution 6 ml intravenously.

      Assessment The general clinical data of the two groups were recorded, including age, sex,
      acute physiology and chronic health status scoring system II (APACHE II), qSOFA, and the
      constituent ratio of primary disease before treatment.

      The inflammatory reaction indexes of the two groups were detected before and on the 7th day
      of treatment, including Interleukin 6 (IL-6), interleukin 8 (IL-8), tumor necrosis factor
      (TNF-α) and endothelin-1 (ET-1). After collecting 5ml of fasting elbow vein blood from two
      groups of patients, the serum was separated by centrifugation (centrifugation radius: 3cm,
      rotation speed: 2000r/min, time: 10min), and then detected by enzyme-linked immunosorbent
      assay (ELISA). ELISA kits for IL-6 (ab178013), IL-8 (ab214030), and TNF-a(ab181241) were
      purchased from abcam company. ELISA kit for ET-1 (K7429-100) was purchased from BioVision.
      All ELISA experiments were performed according to the kit instructions.

      The oxidative stress response indexs of the two groups were detected before and on the 7th
      day of treatment, including superoxide dismutase (SOD), glutathione (GSH), malondialdehyde
      (MDA). The xanthine oxidase method is used to detect SOD, the DTNB method is used to detect
      GSH, and the thiobarbituric acid method is used to detect MDA.

      The renal function indexs before and after treatment were detected before and on the 7th day
      of treatment, including the blood urea nitrogen (BUN) and serum creatinine (sCr) and renal
      resistance index (RRI). RRI was detected by ultrasound.

      And the clinical data, including the rate of renal replacement therapy, ICU length of stay,
      total hospitalization expenses, and 28-d mortality, were recorded.

      Statistical analysis All measurements were expressed as mean ± standard deviation (x ± s).
      And the counting datas were expressed in the form of percentage [n (%)]. The statistical SPSS
      23.0 software were performed using the two samples t-test and adjusted chi-square test for
      the two groups. P-value,0.05 was considered to be statistically significant.
    


Intervention name(s): vitamin B6; 0.9% sodium chloride solution
Intervention type(s): Drug; Drug
Intervention description(s): The Patients in experimental group were given vitamin B6 injection 300mg/d (100mg/2ml× 3) intravenous injection.; That in control group were injected with 0.9% sodium chloride solution 6 ml intravenously.
Intervention MeSH terms: Vitamin B 6; Pyridoxal; Pyridoxine
Allocation: Randomized
Intervention model: Parallel Assignment
Masking: Single (Participant)
Primary purpose: Treatment