The Efficacy of Laser in Root Canal Disinfection
Condition: Pulp and Periapical Tissue Disease; Pulp Necroses; Pulp; Granuloma
MeSH terms: Dental Pulp Necrosis; Granuloma; Necrosis
Study type: Interventional
Phase: Phase 2

Summary: 
      The aim of this study is to assess in vivo the efficacy of Er,Cr:YSGG/diode laser and
      Diode/EDTA on bacterial count in root canal treatment in an evidence-based clinical trial.
      The null hypothesis being tested is that there is no difference in total bacterial count
      reduction between conventional irrigation and the two types of lasers used.

      Thirty patients are equally divided into 3 separate groups :

        -  Group A (Conventional): 2.5% NaOCL and 17% EDTA.

        -  Group B(Dual): saline along with Er,Cr:YSGG laser and diode laser combination

        -  Group C(Combined): saline along with 17% EDTA and diode laser combination
           Microbiological analysis will be done for both aerobic and anaerobic bacteria using
           Colony forming units. All data will be collected, tabulated, summarized, and
           statistically analyzed.
    

Detailed description: 
      The use of lasers in disinfection of the root canal has been recently implemented. Lasers
      have bactericidal effect, and have deep penetration depth inside the root canal up to 1000
      um. Thus, it can be used effectively for disinfection of the root canal system following
      biomechanical instrumentation reaching areas which were considered before non-reachable.

      The aim of this study is to assess in vivo the efficacy of Er,Cr:YSGG/diode laser and
      Diode/EDTA on root canal treatment in an evidence-based clinical trial. The null hypothesis
      being tested is that there is no difference in total bacterial count reduction between
      conventional irrigation and the two types of lasers used.

      Thirty patients are equally divided into 3 separate groups :

        -  Group A (Conventional): 2.5% NaOCL and 17% EDTA.

        -  Group B(Dual): saline along with Er,Cr:YSGG laser and diode laser combination

        -  Group C(Combined): saline along with 17% EDTA and diode laser combination After
           disinfection, local anesthetic and tooth isolation and access cavity preparation, the
           first microbial samples (S1) will be collected using 3 sterile paper points and
           immediately placed inside sterile tubes containing transport medium of thioglycolate,
           cleaning and shaping with final disinfection protocol will be performed according to the
           group in which the participant was allocated to. Followed by S2 sample. obturation using
           warm vertical compaction technique. Microbiological analysis will be done for both
           aerobic and anaerobic bacteria using Colony forming units. All data will be collected,
           tabulated, summarized, and statistically analyzed.
    

Intervention name(s): Conventional group (NaOCl/EDTA); Dual laser group (Er,Cr:YSGG/Diode):; Combined group (EDTA/Diode):
Intervention type(s): Combination Product; Combination Product; Combination Product
Intervention description(s): conventional disinfection using 2.5% sodium hypochlorite and 17% EDTA; Er,Cr:YSGG intracanal laser irradiation to remove smear layer followed by diode laser for disinfection; 17% EDTA was used to remove smear layer followed by diode laser for disinfection
Intervention MeSH terms: Edetic Acid; Pentetic Acid
Allocation: Randomized
Intervention model: Parallel Assignment
Masking: Double (Participant, Outcomes Assessor)
Primary purpose: Treatment
Keywords: LASER; Endodontics; canal disinfection