Comparison Between Intracytoplasmic Morphologically Selected Sperm Injection (IMSI) And Intra Cytoplasmic Sperm Injection (ICSI) Techniques With Eggs Donated
Condition: Sperm Head
Study type: Observational

Summary: 
      This study aims to determine if early embryonic development, in D2 and D3 and the blastocyst,
      is compromised by the fine morphology of sperm nucleus. This will draw the IMSI, in which
      sperm will be chosen using the classification MSOME and ICSI in which sperm morphology is
      elected with less magnification. Both techniques are performed in oocytes of donor , being
      the coohorte used for both techniques picked at random. All courses will begin as a crop
      sequence, using the criteria of the IVF laboratory. Transfers will be made in blastocyst
      stage (D5 or D6), except in cases where the number of fertilized is less than 6 or fewer than
      6 embryos of acceptable quality in D3, which would transfer the same D3 . With donor oocytes,
      the quality of these need not be compromised and to perform the same technique in all oocytes
      results in pure transfers with both techniques. It allows us to compare results in term of
      pregnancy, implantation and early abortion rate, as well as embryo quality.
    

Detailed description: 
      ICSI is the most frequent insemination method used at all IVI clinics. This technique allows
      the observation of both, oocytes and spermatozoa, at a maximum magnification of 400X. At this
      magnification we are only able to assess gross morphological characteristics such as: double
      heads, double tails, globozoospermia, megalozoospermia.

      Nowadays, many groups are using a new method to select spermatozoa known as MSOME (high
      magnification motile sperm organelle morphology examination) (Bartoov B, et al.,2002). This
      method allows us to perform a fine assessment of the sperm nucleus and to select at a very
      high magnification and in real time. The magnification used is between 600 and 13500x.

      Later the group of Bartoov introduced the IMSI (intracytoplasmic morphologically selected
      sperm injection) (Bartoov B., et al., 2003), allowing the single microinjection of selected
      motile spermatozoa with strictly defined morphologically normal nuclei into the cytoplasm of
      retrieved oocytes. This prospective controlled study, performed in couples with male
      infertility and at least two previous failed ICSI attempts, showed that IMSI resulted in a
      significantly higher pregnancy and implantation rate as compared with conventional ICSI. From
      their results they assume that in the IMSI, the survival of the embryo in the uterus is
      associated with the fine morphological state of the sperm nucleus.

      Following these data have been some groups, such as Antinori M. et al., 2007, or
      Vanderzwalmen P et al., 2007 and 2008, showing spectacular results in pregnancy and
      implantation rate with this new technique, in couples who had several unsuccessful cycles of
      assisted reproduction. One objection of this work is that all comparisons have been made by
      relating the results of the IMSI with retrospective results of previous cycles of ICSI.

      This study aims to determine if early embryonic development, in D2 and D3 and the blastocyst,
      is compromised by the fine morphology of sperm nucleus. This will draw the IMSI, in which
      sperm will be chosen using the classification MSOME and ICSI in which sperm morphology is
      elected with less magnification. Both techniques are performed in oocytes of donor , being
      the coohorte used for both techniques picked at random. All courses will begin as a crop
      sequence, using the criteria of the IVF laboratory. Transfers will be made in blastocyst
      stage (D5 or D6), except in cases where the number of fertilized is less than 6 or fewer than
      6 embryos of acceptable quality in D3, which would transfer the same D3 . With donor oocytes,
      the quality of these need not be compromised and to perform the same technique in all oocytes
      results in pure transfers with both techniques. It allows us to compare results in term of
      pregnancy, implantation and early abortion rate, as well as embryo quality.
    

Observational model: Case-Control
Time perspective: Prospective
Sampling method: Probability Sample
Study population: 
        - Egg donation cycles