Efficacy of VHM After Treatment Interruption in Subjects Initiating ART During Acute HIV Infection
Condition: Acute HIV Infection
MeSH terms: Infections; Communicable Diseases; HIV Infections; Acquired Immunodeficiency Syndrome
Study type: Interventional
Phase: Phase 1/Phase 2

Summary: 
      This study is a two-arm prospective 1:1 randomised controlled trial comparing the proportion
      of patients between:

      Group 1: vorinostat/hydroxychloroquine/maraviroc (VHM) co-administered with anti-retroviral
      therapy (ART) Group 2: ART only who are able to maintain HIV RNA < 50 copies/ml following
      treatment interruption. Subjects will be recruited from RV254/SEARCH 010, an acute HIV
      infection cohort conducted by the Thai Red Cross AIDS Research Centre in Bangkok, Thailand.
      The study will run for a minimum of 34 weeks from screening.
    

Detailed description: 
      Study site:

      This will be a single-center proof-of-concept study in which recruitment and follow-up of
      volunteers will be done at the Thai Red Cross AIDS Research Centre (TRC-ARC).

      Subject Population:

      Subjects aged 18-60 years old, who initiated ART during acute HIV infection and have
      maintained viral suppression (HIV RNA < 50 copies/ml) for at least 28 weeks prior will be
      asked to enroll in the study. The subjects must have CD4 ≥ 450 cells/µl, and EKG and
      laboratory values within acceptable ranges.

      Sample Size:

      Fifteen subjects will be enrolled randomized 2:1 to VHM + ART (N=10) versus ART (N=5) only.

      Study Design An exploratory, open label, randomized study of
      vorinostat/hydroxychloroquine/maraviroc (VHM) + ART versus ART only.

      Study Drug Vorinostat will be administered at 400mg orally every 24h for 3 cycles, each of 14
      days with an interim rest-period of 14 days between each cycle over a period of 10 weeks.
      Hydroxychloroquine (HCQ) will be administered at a dose of 200mg 2X/daily during the course
      of vorinostat administration for 10 weeks. Maraviroc will be administered at 600 mg 2X/daily
      on the same schedule as HCQ. This dose of maraviroc is based on its concomitant use with
      efavirenz. Dosing will be adjusted as appropriate should the subject be on an integrase
      inhibitor or a protease inhibitor instead of efavirenz due to intolerance to the drug or
      primary non-nucleoside reverse-transcriptase inhibitor (NNRTI) resistance. Any standard ART
      regimen may be used. However, it is expected that the majority of subjects will be on 2
      nucleos(t)ide reverse-transcriptase inhibitors (NRTI) [emtricitabine (FTC) and tenofovir
      (TDF) and 1 NNRTI [efavirenz (EFV)]. ART will be administered at the following doses: FTC,
      200mg 1X/day or 3TC, 300mg 1X/day; TDF, 300mg 1X/day and EFV, 600mg 1X/day.

      In subjects on NNRTI-based therapy, the NNRTI will be interrupted at week 8 and the rest of
      the regimens will be interrupted at week 10. In order to prevent NNRTI resistance, protease
      inhibitor replacement therapy with darunavir (900 mg 1X/day with ritonavir 100 mg 1X/day)
      will be given between weeks 8 and 10 and maraviroc will be reduced from 1200mg/day to
      300mg/day.

      Study Duration on Protocol:

      A minimum of 34 weeks from treatment initiation. Subjects must have been on ART for a minimum
      of 42 weeks prior to study entry. Note that some subjects may be enrolled from
      RV254/SEARCH010 who have already fulfilled the minimum 42-week ART requirement. The VHM
      treatment will occur over 10 weeks and the follow-up period will be 24 weeks.

      Study Design and Methodology:

      The study design is a two-arm, open label randomized study. Subjects will be recruited from
      RV254/SEARCH 010. RV254/SEARCH 010 is an acute HIV infection cohort conducted by the Thai Red
      Cross AIDS Research Centre in Bangkok, Thailand. Subjects will be followed twice daily for
      the first 24h following the first treatment administration and then at weeks 1, 2, 4, 5, 6, 8
      and 10 and weekly thereafter to week 22, and every 2 weeks thereafter until week 34.
      Phlebotomy will be performed for clinical hematology, CD4, HIV RNA, ALT, creatinine and
      lipids and those in the VHM arm will also receive an EKG and eye examination at screening
      and/or enrollment. Subjects will be monitored weekly following treatment interruption for the
      following 12 weeks for viral load and every two weeks to week 34 to ensure that there is no
      viral rebound, defined as 2 consecutive viral load measurements of >1000 copies/ml. ART will
      be re-initiated and CD4 counts measured in the event of viral rebound.

      A smaller proviral reservoir and less diverse viral population occur in early HIV infection
      relative to chronic infection. Preliminary data from the RV254/SEARCH 010 study indicate that
      T cell immunity is better preserved in subjects treated with ART during early HIV infection
      relative to chronic HIV infection.

      The investigators hypothesize that subjects treated with ART during early HIV infection and
      further treated with multiple cycles of the histone deacetylase inhibitor (HDACi),
      vorinostat, in combination with hydroxychloroquine and maraviroc will lead to activation of
      the latent reservoir and reduce virus infection of new targets. Furthermore, as subjects were
      treated with ART early in HIV infection, the T cell response will be capable of eliminating
      virus activated by the HDACi and result in a reduction of the viral reservoir.

      Primary Objective:

      To compare the proportion of patients between VHM co-administered withART versus ART only
      arms who are able to maintain HIV RNA < 50 copies/ml following treatment interruption.

      Secondary Objectives:

        1. Time to HIV RNA rebound after treatment interruption between VHM +ART versus ART only
           arms

        2. To compare the cell-associated HIV RNA (multi-spliced and unspliced) in total CD4+ T
           cells between the VHM +ART versus ART only arms

        3. To compare markers of HIV persistence (total and integrated HIV DNA and 2-LTR circles)
           between the VHM + ART versus ART only arms

        4. To compare histone acetylation (H3) between the VHM +ART versus ART only arms

        5. To compare adverse events both related and unrelated to the combination of vorinostat,
           hydroxychloroquine and maraviroc between arms

        6. To compare the occurrence and severity of acute retroviral syndrome between arms
           following treatment interruption

      Hypotheses: Compared to the ART only arm, the VHM +ART arm will have:

        1. A higher proportion of patients with HIV RNA < 50 copies/ml following treatment
           interruption at the end of the study

        2. Longer time to HIV RNA rebound following treatment interruption

        3. Higher cell-associated RNA in total CD4+ T cells at the end of the VHM treatment period

        4. Lower reservoir size and 2 LTR circles at the end of the VHM treatment period and the
           end of the study

        5. Higher histone acetylation at the end of the VHM treatment

        6. Higher adverse events related to VHM

        7. Similar rates of acute retroviral syndrome after treatment interruption in subjects
           experiencing viral rebound
    

Intervention name(s): Vorinostat; Hydroxychloroquine; Maraviroc; Tenofovir; Emtricitabine; Efavirenz; Darunavir
Intervention type(s): Drug; Drug; Drug; Drug; Drug; Drug; Drug
Intervention description(s): Vorinostat (suberoylanilide hydroxamic acid) inhibits histone deacetylases class I and II. Vorinostat is supplied as 100mg capsules and will be administered at 400mg/ day in 2 week cycles beginning at week 0 for 10 weeks - 42 doses.; Hydroxychloroquine is supplied as 200mg tablets and will be administered at week 0 for 10 weeks; Maraviroc will be administered at 150 to 600mg/ml twice daily depending on the subject's ART regimen at week 0 for 10 weeks; NRTI. Tenofovir will be administered at 300mg 1 X day at week 0 for 10 weeks; NRTI. Emtricitabine will be administered at 200mg 1 X day at week 0 for 10 weeks; NNRTI. Efavirenz will be administered at 600 mg 1 X day at week 0 for 10 weeks; Protease Inhibitor. Darunavir will be administered at a dose of 900mg 1 X day for subjects on NNRTI based ART beginning at week 8 until week 10
Other intervention name(s): Zolinza; Plaquenil; Selzentry; Viread; Emtriva; Sustiva; Prezista
Intervention MeSH terms: Tenofovir; Emtricitabine; Efavirenz; Maraviroc; Darunavir; Hydroxychloroquine; Vorinostat
Allocation: Randomized
Intervention model: Parallel Assignment
Masking: None (Open Label)
Primary purpose: Treatment
Keywords: Treatment interruption; Controlling HIV