Evaluation of Oxidative Stress and Inflammation Markers in Temporomandibular Disorders
Condition: Temporomandibular Disorder; Oxidative Stress; Inflammation
MeSH terms: Temporomandibular Joint Disorders; Temporomandibular Joint Dysfunction Syndrome; Inflammation
Study type: Observational

Summary: 
      Background: The occurrence of many diseases has been associated with oxidative stress and
      disruption of antioxidant mechanisms. Temporomandibular Joint(TMJ) Disorders are also thought
      to develop with the onset of the destructive process in the tissues as a result of this
      balance being disrupted.

      Purpose: This study was designed to compare salivary and serum oxidative stress and
      inflammation markers of individuals with Temporomandibular Disorders (TMD) and healthy
      subjects.

      Study design, setting, and sample: A prospective cross-sectional study was conducted. 27 TMD
      patients diagnosed with disc displacement (DD) according to RDC/TMD and 17 healthy subjects
      were enrolled in the study. Prior to any treatment, serum and saliva samples were taken from
      the patients and centrifuged, and stored at -80°C until analyzed. All samples were examined
      for IL-6, MDA and 8-OHdG concentrations.

      Predictor variable: Diagnosis of disc displacement (RDC/TMD) Main outcome variables: Levels
      of 8-OHdG, IL-6 and MDA Covariates: Age and gender
    

Detailed description: 
      The term oxidative stress is defined as any condition that causes free radical accumulation
      in tissue. Oxidative stress, which is an indicator of the deterioration of the balance
      between oxidant/antioxidant in an organism, is thought to play a role in the pathogenesis of
      various diseases such as atherosclerosis, aging, cancer, neurological disorders, diabetes,
      ischemia/reperfusion, Alzheimer's, Parkinson's, rheumatoid arthritis and chronic
      inflammation. Recently, many studies have shown a relationship between the pathogenesis of
      TMD and various free radicals, antioxidant enzymes and inflammation mediators. Through
      biomolecule methods studies determine the increase of some of cytokines such as Interleukin
      1β (IL-1β), IL-6 in TMJ with internal derangement. These studies emphasize the positive
      relationship between the severity of the disease and the levels of synovial fluid mediators
      in joints with TMD. Although it has been supported by previous studies that synovial fluid is
      a reliable diagnostic material-that reflects the pathological condition in the joint, the
      disadvantages of the difficulty of obtaining has led to the need for the use of another
      realiable diagnostic tool. Saliva and blood are materials that are easily available and
      provide information about the formation and etiology of many systemic diseases.

      The aim of this study is to compare the salivary and serum oxidative stress and inflammatory
      marker levels of TMD patients with RDC/TMD type II disorder with healthy subjects. For this
      purpose, IL-6, MDA and 8-OHdG will be evaluated as biomarkers of inflammation and oxidative
      stress.
    

Intervention name(s): Biochemical analysis
Intervention type(s): Other
Intervention description(s): Oxidative stress and inflammation markers were analyzed in serum and saliva of TMD and healthy subjects.
Observational model: Other
Time perspective: Prospective
Sampling method: Probability Sample
Study population: 
        TMD subjects who had RDC/TMD type II disorder (TMJ disc displacement (DD) with reduction,
        TMJ-DD without reduction with limited opening, TMJ-DD without reduction without limited
        opening) and, age and sex matched volunteers were included as the control group
      
Biospecimen description: 
      saliva serum