Investigation of The Effectiveness of Antioxidant Therapy in Oligoasthenoteratozoospermic Infertile Men
Condition: Male Infertility
MeSH terms: Infertility; Infertility, Male
Study type: Interventional

Summary: 
      Approximately 30% of the factors that cause male infertility are due to idiopathic causes.
      Increased reactive oxygen species (ROS) due to many known and unknown factors cause male
      infertility by affecting spermatogenesis and sperm maturation. In this study, the effects of
      physical activity and antioxidant food supplementation on seminal antioxidant capacity, sperm
      DNA fragmentation index, sperm chromatin quality and sperm parameters were investigated in
      infertile cases.
    

Detailed description: 
      Material and Method: The study included subfertile men with idiopathic
      oligoasthenoteratozospermia seen at Ondokuz Mayıs University Faculty of Medicine between
      March 2021 and November 2021.

      All subjects were recommended to do moderate physical activity for at least 45 minutes (at
      least 150 minutes-600 METs per week) 3-4 days a week for three months. A total of 48 cases
      were divided into two groups by computer-assisted (www.randomizer.org) complete (simple)
      randomization. In the first group (Group 1), 2000 mg L-carnitine, 2000 mg fructose, 932 mg
      acetyl L-carnitine, 225 mg vitamin C, 115 mg citric acid, 50 mg coenzyme Q10, 14 mg zinc, 115
      µg selenium, 3750 µg Food supplement containing vitamin B12 and 500 µg folic acid was
      recommended as one sachet in the morning and evening, while antioxidant food supplement was
      not given to the second group (group 2). Before and after treatment, semen parameters,
      Hormone analyzes with ELISA method, physical activity evaluation with IPAQ questionnaire,
      seminal antioxidant capacity with Trolox equivalent antioxidant capacity (TEAC) measurement
      method, DNA fragmentation index with TUNEL method. and sperm chromatin structure was
      evaluated by aniline blue staining.

      Student's t test was used for the variables showing normal distribution in independent
      groups, and Mann Whitney U test was used for the variables that did not fit the normal
      distribution. Paired t test was used for the variables showing normal distribution in the
      dependent groups, and Wilcoxon test was used for the variables that were found not to fit the
      normal distribution.
    

Intervention name(s): proxeed plus
Intervention type(s): Combination Product
Intervention description(s): Group 1 was recommended to receive a food supplement containing 2000 mg L-carnitine, 2000 mg fructose, 932 mg acetyl L-carnitine, 225 mg vitamin C, 115 mg citric acid, 50 mg coenzyme Q10, 14 mg zinc, 115 µg selenium, 3750 µg vitamin B12 and 500 µg folic acid as one sachet in the morning and evening
Allocation: Randomized
Intervention model: Parallel Assignment
Intervention model description: two groups with full (simple) randomization
Masking: Double (Participant, Investigator)
Primary purpose: Supportive Care
Keywords: exercise; antioxidant; antioxidant capacity; sperm DNA fragmentation; protamine