Developmental Adaptation to an Obesogenic Environment
Condition: Obesity
Study type: Interventional

Summary: 
      The purpose of this study was to evaluate whether birth weight is related to the demand for
      dietary protein in adult survivors of severe acute malnutrition (Kwashiorkor and Marasmus),
      and that leveraging of protein intake is associated with total energy intake and weight
      change in the survivors consuming foods with different percentages of energy as protein
      (PEP).
    

Detailed description: 
      Differences in birth weight between children with kwashiorkor (K) and marasmus (M) suggest
      that intrauterine factors influence the development of these two syndromes of severe acute
      malnutrition (SAM). Such an effect could play a role in setting specific targets for dietary
      protein that has been proposed to influence total energy intake (TEI), body weight and risk
      of obesity.

      We recruited 63 adult survivors of SAM, aged 17 to 46 years, who in childhood had been
      treated at the Tropical Metabolism Research Unit (TMRU) Metabolic Ward. Data on birth weight,
      age, weight and length at admission were abstracted from TMRU records. Subjects provided
      written informed consent. The study was approved by the Faculty of Medical Sciences Ethics
      Committee, University of the West Indies.

      Measurements were conducted in two phases. All subjects were seen in single-sex pairs and
      stayed in a dedicated metabolic suite for 9 consecutive days. During phase 1 (days 1 to 3-
      choice experiment) they ate freely at each mealtime from menus comprising a combination of
      foods containing different percentages of energy as protein (PEP), set at 10%, 15% or 25%
      (26). During phase 2 (days 4 to 8), pairs were randomized to one of three groups each of
      which received only foods that contained 10%, 15% or 25% PEP. The participants were blinded
      to the treatment. Participants were taken for a 1-hour supervised walk each day at 4 pm.

      Thirty-one local recipes of 10 sweet and 21 savoury foods were selected. Each was modified
      into three recipes containing 10, 15 or 25% energy as protein through the addition of food
      ingredients, a protein mix and/or maltodextrin (Ross Nutrition). Carbohydrate was adjusted to
      be 60, 55 or 45% energy and dietary fat was kept constant at 30%. The PEP versions of each
      food/recipe were taste tested for the ability to determine the protein concentration of any
      dish due to appearance, smell or texture as well as for pleasantness. If taste testers were
      able to detect any difference, the recipes were adjusted and retested until no difference was
      detected. Up to 11 foods were provided on each day during the 8-day period, giving
      participants both variety and choice at all times as indicated in table 1. During the first
      three days (phase 1), three food items other than fruit and vegetable salads and tea were
      offered at breakfast, lunch and dinner to all the participants. These three food items at
      each of these meal times included foods containing 10%, 15% or 25% protein energy. From days
      4 to 8 (phase 2), these same daily food types were repeated every three days but the foods
      contained 10 PEP in one group, in another group they all contained 15 PEP, and in the third
      group they all contained 25 PEP protein.

      Breakfast was provided at 8 am, lunch at 12:30 pm and dinner at 6 pm. During both phases
      snack items were made freely available at all times. The amount eaten was determined by
      weighing each food item before consumption and any not eaten to the nearest gram. The foods
      were served in weighed quantities on tared containers. Plates were of a single design and
      neutral (white) color. The same size, style and color plates were used for the 10, 15 and 25
      PEP version of each food. Participants were offered optional foods including 100g fruit
      salad, and decaffeinated tea sweetened with a measured amount of brown sugar with breakfast,
      and 100g vegetable salad with lunch and dinner. A three-day food diary completed prior to the
      8 day ad libitum test period and foods consumed during the test period were analysed for
      total energy, protein, carbohydrate and fat content.

      The weight of the subjects without shoes and in light clothing was measured daily to the
      nearest 0.1 kg; height was measured to the nearest 0.1 cm. Fat and fat-free mass were
      obtained from DEXA measurements. Weight gain was calculated from day 1 to 4 in phase 1 and
      from day 4 to 8 in phase 2.
    

Intervention name(s): Dietary protein
Intervention type(s): Other
Intervention description(s): Persons were fed
Participants were randomized to one of three arms, each of which received 10%, 15% or 25% of total energy derived from protein
Other intervention name(s): macronutrient
Allocation: Randomized
Intervention model: Parallel Assignment
Masking: Single (Participant)
Primary purpose: Basic Science
Keywords: macronutrient; malnutrition; birth weight; protein leverage