Exercise on microRNA in Rheumatoid Arthritis
Condition: Rheumatoid Arthritis
MeSH terms: Arthritis; Arthritis, Rheumatoid
Study type: Interventional

Summary: 
      The goal of this prospective cohort study is to learn about whether exercise alters microRNA
      expression levels in 30 rheumatoid arthritis patients and 30 healthy controls.

      The main questions it aims to answer are:

        -  Does exercise affect microRNA expression levels related to the pathophysiology of
           rheumatoid arthritis?

        -  Are microRNA levels correlated with disease activity? Participants was applied program
           consisting of strengthening and stretching exercises 2 days a week for 8 weeks. One day
           a week, 30 minutes of mild moderate walking was requested.

      Researchers will compare rheumatoid arthritis patients and healthy controls to see if that
      the microRNA expression levels of patients with rheumatoid arthritis as a result of exercise
      training would not make a significant difference with healthy controls.
    

Detailed description: 
      Rheumatoid arthritis can be a systemic, inflammatory, autoimmune breathing that can cause
      heavy, joint destruction with vibrations in the synovial vessels of the joints. Although
      great strides have been made in understanding the disease, the pathogenesis of Rheumatoid
      arthritis.A has not yet been fully elucidated. Early treatment can prevent serious
      disability.

      The goal of treatment for patients with rheumatoid arthritis is to achieve remission or at
      least a state of low disease activity.Combinations with disease-modifying antirheumatic drugs
      (DMARDs) and biologic agents are recommended for patients who fail to respond to treatment,
      based on patient prognosis and response to treatment.

      In addition to drug therapy, exercise training is recommended in patients with Rheumatoid
      arthritis. Decreased joint health, including joint pain and inflammation, fatigue, increased
      incidence of cardiovascular disease in patients with rheumatoid arthritis; it causes
      functional limitation, disability, comorbidities and a decrease in quality of life.Therefore,
      it is recommended that aerobic and resistance exercise training should be encouraged as part
      of the routine treatment of all Rheumatoid arthritis patients.

      microRNAs (miRNAs) are small, non-coding RNAs that mediate messenger RNA (mRNA) cleavage,
      translational repression. As regulators of gene expression, miRNAs participate in critical
      cellular processes and show varying levels of expression in many cell types in different
      diseases.It became clear that there were changes in miRNA expression in Rheumatoid arthritis
      patients.In many studies, it has been shown that miRNAs contribute to the disruption of joint
      destruction, inflammation and apoptosis in peripheral blood mononuclear cells, isolated T
      lymphocytes, synovial tissue and synovial fibroblasts. Thus, miRNAs are known to constitute
      the pathophysiological process specific to Rheumatoid arthritis.

      miRNA-146a is one of the miRNAs that has been extensively studied in Rheumatoid arthritis. It
      was found that the expression of miRNA-146a in the synovial fluid and synovial tissue of
      Rheumatoid arthritis A patients was significantly higher than in healthy individuals, and
      miRNA-16 and miRNA-155 together with miRNA-146a may be related to disease pathology. In
      addition, high levels of miRNA-16 expression are known to correlate with active disease and
      low expression levels with remission. It has been reported that the expression level of
      miRNA-145 is increased in peripheral blood mononuclear cells and synovium of Rheumatoid
      arthritis patients, which supports osteoclastogenesis.

      In this study, it was aimed to determine whether miRNAs, which play a role in determining the
      effectiveness of the treatment and the severity of the disease, change with exercise
      training, to know the effect of exercise training on the person, to accept it as an auxiliary
      practice in controlling the disease and to prove it with scientific facts.
    

Intervention name(s): exercise; gene expressions of miRNA
Intervention type(s): Behavioral; Biological
Intervention description(s): A program consisting of strengthening and stretching exercises 2 days a week was applied to the study group for 8 weeks. One day a week, 30 minutes of mild moderate walking was requested.; In the samples taken, gene expressions of miRNA-146a, miRNA-155, miRNA-16, miRNA-145 were determined by real-time polymerase chain reaction method.
Intervention model: Single Group Assignment
Masking: None (Open Label)
Primary purpose: Treatment
Keywords: Rheumatoid arthritis; exercise; microRNA