The Effects of Whey, Rice and Potato pRotein isOlates on appeTite rEgulatIoN in Healthy Men
Condition: Appetite and General Nutritional Disorders
MeSH terms: Nutrition Disorders
Study type: Interventional

Summary: 
      INTRODUCTION: Global protein consumption has been increasing for decades due to changes in
      socio-economic demographics and consumer trends towards higher protein intake, which may be
      related to their health benefits in performance nutrition and appetite regulation. The
      additional intake of protein often occurs in the form of supplements. One of the main
      ingredients used in sports and weight loss supplements are animal-derived proteins such as
      whey, which are of high quality, containing all the essential amino acids required in human
      nutrition with a high absorption rate. However, whey protein has a high environmental
      footprint, and it may contribute to insulin resistance in susceptible groups (e.g. obese,
      T2DM, insulin resistant, or those with sarcopenic obesity).

      Rice and potato protein isolates are high-quality plant proteins containing all essential
      amino acids comparably to whey protein. These plant-derived proteins may provide a more
      sustainable and accessible alternative to animal-derived proteins for appetite regulation and
      weight management.
    

Detailed description: 
      Intervention study with three treatment groups in a randomised, single-blind, triple
      crossover design.

      Study population: Healthy male participants will receive three different isocaloric and
      -volumetric protein shakes prepared using whey, rice and potato protein powders on different
      trial days with at least a one-week washout period apart.

      Biological samples (blood and saliva samples) are collected at various time points during the
      visit. Blood samples are collected at baseline 10 min prior (T-10), then at 30 min (T30), 60
      min (T60), 120 min (T120) and at 180 min (T180) after protein shake consumption. Saliva
      samples are collected at baseline (T-10), then at every 30 minutes following protein shake
      consumption (T30, T60, T90, T120, T150, T180). In addition, pertinent questionnaires Visual
      Analogue Scale [VAS] for the assessment of satiety and VAS for the assessment of protein
      shakes' perception will be collected at T-10, T30, T60, T90, T120, T150, T180 and at T0,
      respectively
    

Intervention name(s): Whey protein isolate; Potato protein isolate; Rice protein isolate
Intervention type(s): Dietary Supplement; Dietary Supplement; Dietary Supplement
Intervention description(s): Instantized BiPRO® 50g (equiv. 45.5g of total protein) presented to participants in a form of a shake.
Other ingredients in the whey protein shake:
Tropicana Trop50 Orange Juice 207mL
Water 43 mL
Sucralose 0.5g
The total energy 233.47Kcal.
Participants instructed to consume the entire protein shake presented to them in 5 minutes on an empty stomach when attending the study.; Solanic®100 48g (equiv. 45.3g of total protein) presented to participants in a form of a shake.
Other ingredients in the potato protein shake:
Tropicana Trop50 Orange Juice 250mL
Sucralose 0.5g
The total energy 233.46Kcal
Participants instructed to consume the entire protein shake presented to them in 5 minutes on an empty stomach when attending the study.; Oryzatein® Silk 90 50g (equiv. 45.5g of total protein) presented to participants in a form of a shake.
Other ingredients in the rice protein shake:
Tropicana Trop50 Orange Juice 183mL
Water 67mL
Sucralose 0.5g
The total energy 233.43Kcal
Participants instructed to consume the entire protein shake presented to them in 5 minutes on an empty stomach when attending the study.
Allocation: Randomized
Intervention model: Crossover Assignment
Intervention model description: single-blind, randomised, triple crossover
Masking: Single (Participant)
Primary purpose: Basic Science
Keywords: nutraceutical; protein; whey; potato protein; rice protein; appetite; satiety; insulin; glucose; appetite hormone; GLP-1; PYY; supplement