Effect of 8-week Supplementation With Anthocyanin Compounds Contained in Black Chokeberry on Post-workout Recovery in Highly Trained Rowers
Condition: Inflammation;Muscle(S); Oxidative Stress Injury; Permeability; Increased
MeSH terms: Myositis; Inflammation
Study type: Interventional
Phase: Phase 4

Summary: 
      Purpose: To analyze the effects of 8-week supplementation with anthocyanin compounds
      contained in black chokeberry (Aronia melanocarpa) on indicators of inflammation and
      oxidative stress.

      Hypothesis: Supplementation with anti-inflammatory and antioxidant anthocyanin compounds
      improves recovery from intense exercise by reducing inflammation and oxidative stress in
      highly trained rowers

      The study consisted of a nutritional intervention (supplementation) - with compounds of
      natural origin - chokeberry extract (capsules) - 18% standardized for anthocyanin content
      (dose 3 x 200 mg per day) - that is, 107 mg of pure anthocyanins per day, or a placebo
      product that was made from chokeberry fiber

      Study plan I term of the study

        1. Conduct a body composition analysis, 24 hour dietary recall

        2. Collection of blood samples before the exercise test for biochemical determinations.

        3. Exercise test of 2000m on a rowing ergometer.

        4. Collection of blood immediately after exercise and 1 hours after

        5. Collection of blood for biochemical determinations 24 hours after the end of the
           exercise test

        6. Supplementation for 8 weeks 2nd test date Repeat the measurements from the 1st test
           date.

      Participants: Youth National Rowing Team of Poland Experimental procedure: Observation of the
      effect of black chokeberry consumption on parameters of inflammation, oxidative stress and
      intestinal parameters during the immediate start preparation period in highly skilled rowers.
    

Detailed description: 
      Participants Twenty male from the Youth National Rowing Team of Poland have been recruited
      for the study. In the end, sixteen rowers participated in the study (two did not show up for
      the first date, two more were absent for the second date). Before the first and second dates
      of the study, a body composition analysis was performed using the TANITA MC-780 with an
      accuracy of 0.05 kg. The results are shown in Table 1. The experiment was conducted in
      accordance with the Declaration of Helsinki. The study protocol was approved by the Ethical
      Committee of the Medical University of Poznan (resolution no. 390/22). All players were
      informed of the exact course of the study and gave written consent to participate.

      Youth National Rowing Team of Poland The athletes' diets were completely analyzed before each
      exercise test by a nutritionist. Participants, with the help of a dietician who was available
      during meals on test days, filled out questionnaires. The amount of energy, protein,
      carbohydrates and fats were then analyzed using a commercially available program.

      Experimental procedure The rowers were randomly divided into two groups-supplemented and
      control, it was a double-blind study. Athletes in the supplemented group (n=7) consumed
      capsules with highly concentrated 18% chokeberry extract. One capsule contained 200 mg. In
      addition to chokeberry extract, each capsule also contained chokeberry fiber, E460b magnesium
      salts of stearic acid (anti-caking agent), E551 silicon dioxide (anti-caking agent),
      hydroxypropyl methylcellulose (capsule shell) and E171 titanium dioxide (shell color). The
      control group (n=8) consumed capsules that were made from chokeberry fiber. All additional
      substances the same as in capsules with chokeberry extract. The supplement and placebo
      product was manufactured by MBL BIOTRADE, Poznań. The rowers (both supplemented and control
      group) took 3 capsules a day (at breakfast, lunch and dinner) for 8 weeks. The athletes
      sipped each capsule with a glass of water.

      Exercise test On two testing dates (before and after supplementation), the athletes performed
      a 2,000-meter test on a rowing ergometer (Concept II, USA). Athletes had to complete the test
      in the shortest possible time overcoming the maximum load. The results of the test were taken
      into account in the selection for the championship team so the athletes performed the task
      with great motivation. Before the test, each competitor individually performed a 5-minute
      warm-up.

      Material collection and examination Samples were taken at three time points: pre (before the
      exercise test) post (immediately after the exercise test and 1 hour after) and recovery (24
      hours after the exercise test) both before and after the supplementation period. Blood
      samples were collected from the elbow vein into 9-ml tubes (to obtain serum) and centrifuged
      at 3,000 rpm and into 2.7-ml tubes (to determine morphology). Morphology was determined on
      the same day the samples were collected, while serum was frozen and stored at -80oC until
      assays were performed. In addition, before and immediately after the exercise test, capillary
      blood was drawn from the earlobe to determine lactate levels.

      Measurements TAC (Total Antioxidant Capacity), TBARS (levels of lipid peroxidation products),
      IL-2, IL-6, IL-10, TNF-α and myoglobin levels were determined using ELISA kits (SunRed
      Biotechnology Company). Lactate levels were determined immediately after capillary blood
      sampling using a commercially available kit (Dr. Lange, Germany). The concentration of
      lactate was determined in mmol/l. intestinal parameters: LBP (lipopolysacharide-binding pro),
      Claudin3, I-FABP (intestinal fatty acid binding pro)

      Any data obtained during the research will be used only for the purposes of this research
      project. The participant has the right to access and correct his/her data, to obtain
      information regarding the processing of his/her personal data, to request the deletion or
      restriction of the processing of his/her data, the right to portability of the data provided,
      the right to object to the processing, and the right to withdraw consent to the processing of
      personal data at any time. Withdrawal of consent does not affect the lawfulness of processing
      carried out on the basis of consent before its withdrawal.

      The confidential nature of the research documentation will be respected throughout the study.
      Legal requirements for the protection of personal data will be observed. In the study
      documentation, participants will be identified by a nadem code at the beginning of the study.
      The results of this study will be published after pooled data analysis, thus the identity of
      the participants will remain confidential.

      The place of data storage is the university's branch office in Gorzow Wielkopolski.

      All parameters will be analyzed using Elisamtests and read on the ELISAMAR reader. In terms
      of statistical measures, the following will be performed: descriptive statistics,
      Shapiro-Wilk test to assess normality, and multivariate analysis of variance ANOVA test.
    

Intervention name(s): black chokeberry (Aronia melanocarpa); Placebo
Intervention type(s): Drug; Drug
Intervention description(s): Black chokeberry capsules standardized for anthocyanin compounds; Chokeberry fiber placebo capsules
Allocation: Randomized
Intervention model: Parallel Assignment
Intervention model description: Participants randomly divided into one of two groups. The supplemented group (n=7) receives chokeberry capsules, while the control group (n=8) receives a placebo product.
Masking: Single (Participant)
Primary purpose: Basic Science