Effects of Beetroot Juice in Postmenopausal and Hypertensive
Condition: Hypertension; Menopause
Study type: Interventional

Summary: 
      The decrease in estrogen in postmenopausal women leads an increase in endocrine metabolic
      dysfunctions, such as changes in lipid profile and blood pressure. Thus, one of the
      alternatives for prevention and treatment is the practice of physical exercise associated
      with diet containing precursors of nitric oxide (NO), which is a potent vasodilator.
      Therefore, this study intends to verify if supplementation of beetroot juice changes the
      hemodynamic and salivary markers in postmenopausal and hypertensive women. Therefore, the
      intervention consists of 350ml of concentrated beetroot juice or placebo, which will be
      ingested in fasting. After 2 hours will be carried out a session of aerobic physical exercise
      in treadmill and blood pressure will be measured each 15 minutes for 90 minutes after the end
      of the session, in addition to the 24-hour measurement that will be performed by an ABPM
      device. Salivary samples will be held: before the juice ingest, right after exercise, 90
      minutes after and 24 hours after the end of the exercise. With the development of this study,
      it is expected to find blood pressure reduction and normalization of the inflammatory profile
      through salivary samples, after the period of supplementation associated with physical
      exercise practice.
    

Detailed description: 
      The intervention protocol will last 3 days. On the first day there will be the collection of
      anthropometric data, the signature of a consent term and delivery of the list of foods that
      should be avoided 24 hours before the tests and a treadmill familiarization. On the second
      and third day the physical intervention will be performed associated with juice or placebo
      supplementation. A wash out period of at least 7 days will be performed between the second
      and third day. The volunteers will arrive at the laboratory at 7am in the fasted state and
      will be rested until 07:20am and then the blood pressure (BP) measurement (collected three
      times, with an interval of 1 minute between measurements), collection of the saliva and the
      measurement of heart rate (HR). Approximately at 07:30am they will start drinking the juice
      and will have up to 15 minutes to consume. BP will be measured at 08:00am, 08:30am, 09:00am
      and 09:30am. At 9:30am a physical exercise session will begin, during which will be monitored
      by a cardio-frequency monitor, which will remain until 11h10am (period referring to the end
      of BP monitoring in the laboratory). At 10:10am the workout will be finished, and then the
      saliva will be collected. After this period, the volunteers can take up to 250 ml of water
      until 11:10am, and every 15 minutes, BP will also be measured. and at 11:10am a collection of
      saliva will be performed. The volunteers will have the period of 15 minutes to be hygienised,
      and at 11:25am the ABPM will be placed (ambulatory blood pressure monitor). The ABPM will be
      taken the day after the test at 10:10 am (time for the first post-exercise BP measurement),
      and the last collection of saliva will be carried out concurrently with the ABPM removal.
    

Intervention name(s): Exercise; Beetroot juice; Placebo
Intervention type(s): Other; Dietary Supplement; Dietary Supplement
Intervention description(s): 40 minutes of aerobic exercise on a treadmill, with a fixed speed of 5.5 km/h and intensity imposed by treadmill inclination until it reaches the frequency zone between 60% and 75% of the reserve heart rate.; Ingestion of 350ml of concentrated beetroot juice (400mg of nitrate) 100 minutes before an exercise session.; Ingestion of 350ml of placebo (0mg of nitrate, with the same color, taste and smell) 100 minutes before an exercise session.
Other intervention name(s): Exercise session
Allocation: Randomized
Intervention model: Crossover Assignment
Intervention model description: The volunteers will do 2 days of intervention in random order with 7 days of wash out. In one of the days will consume 350ml of concentrated beet juice and in the other 350ml of placebo made with the juice itself denitratized by an anionic resin that does not alter taste, smell or color.
Masking: Double (Participant, Investigator)
Primary purpose: Prevention
Keywords: Combined Exercise; Beetroot juice; Women health