Keratinocyte Growth Factor and Cytokines in Burns.
Condition: Burns; Growth Factors, Combined Defect of; Inflammation; Cytokine Storm
MeSH terms: Inflammation; Cytokine Release Syndrome
Study type: Observational

Summary: 
      KERATINOCYTE GROWTH FACTOR AND CYTOKINES IN SKIN BURNS.

      INTRODUCTION: Intense inflammatory responses are activated by burns that affect a large total
      body surface area. Changes in plasma levels of cytokines after burns occur before metabolic
      abnormalities unsettle the patient. So it may be possible to develop therapeutic
      interventions that may attenuate the acute inflammatory response by decreasing the expression
      of these cytokines. The importance of growth factors in the healing process was demonstrated
      in cultured keratinocytes and fibroblasts. The keratinocyte growth factor (KGF) is a growth
      factor active in the repair of wounds, being the most potent stimulator of mitotic cells.

      PURPOSE: To assess the level of keratinocyte growth factor (KGF) and IL-1ß, IL-6, IL-8,
      IL-10, IL-12 and TNF-alfa of patients with burns produced by cultured primary dermal
      fibroblasts and the gene expression.

      METHODS: 10 patients will be include (05 patients in the study group and 05 patients in the
      control group) admitted to the Burns Care Unit of the Discipline of Plastic Surgery, Federal
      University of São Paulo (UNIFESP) between 25% and 50% of total body surface area (TBSA), deep
      second-degree or third degree, with need to perform surgical debridement. The control group
      will be constituted by patients with less than 5% of TBSA, deep second-degree or third
      degree, with need to perform surgical debridement. The authors will evaluate the levels of
      IL-1ß, IL-6, IL-8, IL-10, IL-12p70 and tumor necrosis factor alpha (TNF-alfa) in samples of
      the culture media of primary dermal fibroblasts of patients selected using flow cytometry.
      The level of keratinocyte growth factor (KGF), in the same samples will be evaluated by
      ELISA. The keratinocyte growth factor (KGF) and TNF-alfa gene expression will be evaluated in
      the culture of primary dermal fibroblasts from the same patients. The gene expression of KGF
      and cytokines will be done by qRT-PCR and RT-PCR array. The experiments will be done in
      duplicate.
    

Detailed description: 
      KERATINOCYTE GROWTH FACTOR AND CYTOKINES IN SKIN BURNS.

      INTRODUCTION: Intense inflammatory responses are activated by burns that affect a large total
      body surface area. Changes in plasma levels of cytokines after burns occur before metabolic
      abnormalities unsettle the patient. So it may be possible to develop therapeutic
      interventions that may attenuate the acute inflammatory response by decreasing the expression
      of these cytokines. The importance of growth factors in the healing process was demonstrated
      in cultured keratinocytes and fibroblasts. The keratinocyte growth factor (KGF) is a growth
      factor active in the repair of wounds, being the most potent stimulator of mitotic cells.

      PURPOSE: To assess the level of keratinocyte growth factor (KGF) and IL-1ß, IL-6, IL-8,
      IL-10, IL-12 and TNF-alfa of patients with burns produced by cultured primary dermal
      fibroblasts and the gene expression.

      METHODS: 10 patients will be include (05 patients in the study group and 05 patients in the
      control group) admitted to the Burns Care Unit of the Discipline of Plastic Surgery, Federal
      University of São Paulo (UNIFESP) between 25% and 50% of total body surface area (TBSA), deep
      second-degree or third degree, with need to perform surgical debridement. The control group
      will be constituted by patients with less than 5% of TBSA, deep second-degree or third
      degree, with need to perform surgical debridement. The authors will evaluate the levels of
      IL-1ß, IL-6, IL-8, IL-10, IL-12p70 and tumor necrosis factor alpha (TNF-alfa) in samples of
      the culture media of primary dermal fibroblasts of patients selected using flow cytometry.
      The level of keratinocyte growth factor (KGF), in the same samples will be evaluated by
      ELISA. The keratinocyte growth factor (KGF) and TNF-alfa gene expression will be evaluated in
      the culture of primary dermal fibroblasts from the same patients. The gene expression of KGF
      and cytokines will be done by qRT-PCR and RT-PCR array. The experiments will be done in
      duplicate.

      Key words: burns, cytokines, inflammation, keratinocyte growth factor, fibroblasts.
    

Observational model: Case-Control
Time perspective: Cross-Sectional
Sampling method: Non-Probability Sample
Study population: 
        ACUTE BURNED PATIENTS
      
Biospecimen description: 
      Normal skin will be collected at first burned patient debridment and send to Cell Culture
      Laboratory to start fibroblast and queratinocyte culture.
    
Keywords: burns; Growth Factors, Combined Defect of; inflammation; Cytokine Storm