microRNA 155 Levels and Periodontal Microflora (Bacteria and Virus) in Preeclampsia.
Condition: Preeclampsia; Chronic Periodontitis
MeSH terms: Periodontitis; Chronic Periodontitis; Pre-Eclampsia
Study type: Observational [Patient Registry]

Summary: 
      Preeclamptic women with and without chronic periodontitis were recruited for the study.
      Subgingival plaque samples and placental tissue samples(postpartum) were obtained from the
      participants for the microbial analysis(bacteria, viruses) and MIR155 levels and comparison
      was done among groups. this study was designed in order to evaluate the role of periodontal
      microorganisms in preeclampsia and to find compare the MIR155 levels among participants, so
      as to check its importance as a biomarker for inflammatory disease like preeclampsia.
    

Detailed description: 
      Four hundred forty five pregnant women, aged 18-35 yrs were selected and were divided into
      four groups. Controls- 145 systemically and periodontally healthy, Group A-100 Systemically
      healthy women with chronic periodontitis, Group B- 100 Preeclamptic women with chronic
      periodontitis, Group C-100 Preeclamptic women without chronic periodontitis. Age, BMI,
      Socioeconomic status and periodontal parameters such as plaque index(PI), bleeding on
      probing(BOP), probing pocket dept(PPD), clinical attachment level(CAL) were recorded.
      Subgingival plaque and placental tissues (postpartum) were collected and assessed for
      periodontal pathogens such as Tannerella forsythia(Tf), Treponema denticola(Td),
      Porphyromonas gingivalis(Pg), Prevotella intermedia(Pi), Fusobacterium nucleatum(Fn). Herpes
      Simplex Virus(HSV), Ebstein Barr Virus(EBV), Human Cytomegalovirus(HCMV). microRNA155 levels
      were also assessed and compared between the groups.
    

Intervention name(s): clinical intervention for microbial analysis and MIR155 as biomarker
Intervention type(s): Other
Intervention description(s): Subgingival plaque was collected with sterile curette and placental tissue sample(postpartum with surgical blade with the help of gynecologist. all the samples were subjected to RTPCR for analysis of microorganisms likeTannerella forsythia(Tf), Treponema denticola(Td), Porphyromonas gingivalis(Pg), Prevotella intermedia(Pi), Fusobacterium nucleatum(Fn). Herpes Simplex Virus(HSV), Ebstein Barr Virus(EBV), Human Cytomegalovirus(HCMV). and MIR155 levels were also estimated
Observational model: Case-Control
Time perspective: Cross-Sectional
Sampling method: Non-Probability Sample
Study population: 
        Controls- 145 systemically and periodontally healthy pregnant women, Group A-100
        Systemically healthy pregnant women with chronic periodontitis, Group B- 100 Preeclamptic
        pregnant women with chronic periodontitis, Group C-100 Preeclamptic pregnant women without
        chronic periodontitis.
      
Biospecimen description: 
      subgingival plaque samples and placental tissue samples were obtained. DNA was isolated and
      then subjected to RTPCR for microbial identification and estimation of MIR155 levels.
    
Keywords: periodontal microflora, preeclampsia