Fish Nutrient Supplementation in High-intensity Functional Training
Condition: Exercise Training; Dietary Supplements
Study type: Interventional

Summary: 
      Nowadays, many persons who exercise consume dietary supplements. Previous scientific studies
      have found that protein supplementation increases muscle mass when combined with an exercise
      program. In addition, vitamin D probably improves performance and muscle function. Likewise,
      ω3 fatty acids, apart from improving cardiovascular function, may also increase protein
      synthesis and performance. Thus, the aim of this study is to investigate the efficacy of a
      novel aquaculture-based sport supplement of proteins, ω3 fatty acids and vitamin D on
      physical performance, body composition, and the biochemical profile of persons following
      high-intensity functional training (HIFT). The efficacy of the experimental supplement (E)
      will be compared with whey protein (W) and maltodextrin (a carbohydrate) as placebo (P).

      Thirty healthy trained individuals (15 male and 15 female), aged 18-35, will take E, W, and P
      during three 6-week periods of HIFT (a different supplement each period) with three workouts
      a week and a washout (no supplement) period of 2 weeks between supplementation periods. E is
      a combination of powder, containing protein and vitamin D, and capsules containing ω3 fatty
      acids. When on E, participants will receive 0.6 g protein/kg body weight, 20 μg vitamin D,
      and 1.8 g ω3 fatty acids daily. When on W, they will receive 0.6 g protein/kg body weight
      and, when on P, they will receive 0.6 g maltodextrin/kg body weight daily. The order of E, W,
      and P will be random and counterbalanced. Throughout the study, participants will be on
      isoenergetic nutritional plans to avoid differences in energy intake that might compromise
      the validity of the study. The dietary plans will be individualized and will provided 1.0 g
      protein/kg body weight/day.

      Participants will undergo measurements of muscle strength, muscle endurance, aerobic
      capacity, and body composition prior to the first supplementation period, between periods,
      and after the third period. Also, blood amino acids, fatty acid acids, vitamin D, hematology,
      biochemistry, and hormones will be measured. To avoid bias, assessors will not know which
      supplement each participant is taking during each period.

      The main hypotheses of the study are: 1) Consumption of E will increase lean body mass
      compared to W and P; 2) consumption of E will improve muscle strength, muscle endurance, and
      aerobic capacity compared to W and P; and 3) consumption of E will result in a better blood
      amino acid, fatty acid, and vitamin D profile.
    


Intervention name(s): Fish nutrients; Whey protein; Maltodextrin
Intervention type(s): Dietary Supplement; Dietary Supplement; Dietary Supplement
Intervention description(s): Intake of fish protein, omega-3 fatty acids, and vitamin D; Intake of whey protein; Intake of maltodextrin
Allocation: Randomized
Intervention model: Crossover Assignment
Masking: Triple (Care Provider, Investigator, Outcomes Assessor)
Primary purpose: Other