Donor Recipient Kidney Function Following Open Surgical vs. Laparoscopic Kidney Donation
Condition: Kidney Transplantation
Study type: Observational

Summary: 
      This study will evaluate the differences between open surgical kidney donation and
      laparoscopic kidney donation on kidney donors and recipients. Both procedures are standard
      surgeries used to remove kidneys for donation, and they are done equally often. Open surgical
      kidney donation involves removing the donor kidney through a 3- to 5-inch surgical incision.
      Laparoscopic donation involves making several small holes in the skin and removing the kidney
      through a larger hole, while directly watching the kidney with a camera. The study will
      correlate the effects of both procedures with donor and recipient kidney function, urine
      output, post-operative pain, and return to work after surgery.

      Adults without kidney disease who are willing to donate a kidney to a patient enrolled in a
      clinical transplant protocol at the NIH Clinical Center may be eligible for this study.
      Donors and recipients must be enrolled in the NIDDK protocol, Live Donor Renal Donation for
      Allotransplantation (protocol #99-DK-0107).

      Donors and patients undergo the following procedures:

        -  Infrared imaging (measurement of small differences in temperature using a special
           camera) during surgery to look at blood flow to the kidney during the operation (both
           donor and recipient surgical procedures). The pictures provide images of the blood
           vessels in the kidney and measure how the blood flow changes.

        -  Kidney biopsy (removal of a small piece of kidney tissue). The patient's failed kidney
           is biopsied once during transplant surgery when it is removed. The donor's kidney is
           biopsied twice - once during surgery to remove the organ from the donor and again after
           transplant into the recipient.

        -  Evaluations after surgery of post-operative urine output, blood pressure, and pain, and
           length of hospital stay and return to work.
    

Detailed description: 
      Laparoscopic techniques are increasingly being used for live donor nephrectomy for kidney
      transplantation. The technical methods have been rapidly adopted by the transplant community,
      but remain limited by the requirement for pneumoperitoneum, a technique that increases
      intra-abdominal pressure to gain surgical exposure. Pneumoperitoneum reduces renal cortical
      blood flow, potentially inducing ischemia associated factors that promote allograft rejection
      or attenuate post transplant renal function.

      The aim of this study is to determine the effects of pneumoperitoneum used for minimally
      invasive donor nephrectomy on renal perfusion using infrared imaging. Our goals are to:

        1. Analyze the effect of varying levels of pneumoperitoneum intraoperatively using infrared
           imaging;

        2. Correlate the effects of both open and laparoscopic living donation with respect to
           transcriptional events, donor and recipient renal function, urine output, post-operative
           pain and return to work postoperatively;

        3. Determine optimal conditions for procurement of renal allografts for subjects undergoing
           renal transplantation.

      In this study, patients will be those who have been consented to kidney donation on the Live
      Donor Renal Donation for Allotransplantation (99-DK-0107). This study will represent the
      first such use of infrared imaging and the first prospective evaluation of strategies for
      living kidney donation.
    

Keywords: Renal; Allografts; Nephrectomy; Pneumoperitoneum; LaparoscopIC; Kidney Donation