The Use of the 25 (OH) D Saliva Test as a Substitute for the 25 (OH) D Serum Test in Healthy People
Condition: Nutritional Deficiency
MeSH terms: Malnutrition
Study type: Interventional

Summary: 
      Routine examination of vitamin D levels is carried out by checking serum 25 (OH)D levels,
      which indicate circulating vitamin D levels. While serum 1.25 (OH) D levels are less
      frequently utilized, they represent the active form of vitamin D and could be a substitute
      for checking vitamin D levels. This study aims to find the correlation between vitamin D
      levels, namely 25 (OH) D and 1.25 (OH) D saliva, which correlate with serum 25 (OH) D and
      1.25 (OH) D levels in the examination of salivary vitamin D, and which could be a substitute
      for checking serum vitamin D levels.
    

Detailed description: 
      Routine examination of vitamin D levels is carried out by checking serum 25 (OH)D levels,
      which indicate circulating vitamin D levels. While serum 1.25 (OH) D levels are less
      frequently utilized, they represent the active form of vitamin D and could be a substitute
      for checking vitamin D levels.This study aims to find the correlation between vitamin D
      levels, namely 25 (OH) D and 1.25 (OH) D saliva, which correlate with serum 25 (OH) D and
      1.25 (OH) D levels in the examination of salivary vitamin D, and which could be a substitute
      for checking serum vitamin D levels.This study is a cross-sectional study involving healthy
      men and women, aged 20-50 years, from Lima Puluh Village, Batubara District, North Sumatra
      Province, Indonesia. The parameters studied were 25 (OH) D and 1.25 (OH) D levels of saliva
      and serum.
    

Intervention name(s): not currently consumed vitamin D supplements regularly; consumed vitamin D supplements regularly
Intervention type(s): Diagnostic Test; Diagnostic Test
Intervention description(s): The tests carried out were examination of 25 (OH) D and 1.25 (OH) D levels of serum and saliva, examination of demographic data, and other anthropometries. The examination was carried out by taking 5 mL of blood and 2 mL of saliva, and then checking the serum and saliva levels of 25 (OH) D and 1.25 (OH) D. Before the examination, the research subjects were asked not to consume food or drink for at least 90 minutes before being examined. Centrifugation was carried out (2500 g, 10 minutes) and the product immediately stored at -20 oC. In the serum examination, after the blood was drawn, centrifugation was carried out and the product stored at -20 oC for further determination of the levels of 25 (OH) D and 1.25 (OH) saliva.; The tests carried out were examination of 25 (OH) D and 1.25 (OH) D levels of serum and saliva, examination of demographic data, and other anthropometries. The examination was carried out by taking 5 mL of blood and 2 mL of saliva, and then checking the serum and saliva levels of 25 (OH) D and 1.25 (OH) D. Before the examination, the research subjects were asked not to consume food or drink for at least 90 minutes before being examined. Centrifugation was carried out (2500 g, 10 minutes) and the product immediately stored at -20 oC. In the serum examination, after the blood was drawn, centrifugation was carried out and the product stored at -20 oC for further determination of the levels of 25 (OH) D and 1.25 (OH) saliva.
Intervention MeSH terms: Vitamin D; Ergocalciferols; Cholecalciferol; Vitamins
Allocation: Randomized
Intervention model: Parallel Assignment
Masking: Single (Participant)
Primary purpose: Diagnostic
Keywords: vitamin D; cross-sectional; non-invasive; correlation; deficiency; insufficiency