Human Papillomavirus (HPV) Genotypes in Genital Warts in Colombian Population
Condition: Genital Warts; HPV Infections
MeSH terms: Warts; Papillomavirus Infections; Condylomata Acuminata
Study type: Observational

Summary: 
      HPV infection has been recognized as the most prevalent sexual transmitted infection
      worldwide. Burden of many HPV related diseases is well known particularly regarding the
      different associated cancers (cervical, anal, vaginal, vulvar, oro-pharyngeal) (3) ; however,
      there is much less available information on the general burden of genital warts, the
      associated HPV types, the costs of related medical treatments and the knowledge and
      perceptions towards the disease and its prevention.

      Objectives:

        1. To determine the HPV genotypes distribution in genital warts in the study population.

        2. To assess the knowledge about transmission & prevention of HPV infection related
           diseases

        3. To determine the direct medical costs of genital warts attention.
    

Detailed description: 
      Study population

      Female and male between 18 - 45 years of age living in Bogota, Colombia

      Sample frame

      Patients attending to gynecologic or STD consult in a mayor out patient clinic in Bogotá
      (PROFAMILIA)

      Analysis

      Analysis of the information will be exhaustive. We will calculate prevalence of HPV genotypes
      in the GW in both sexes separately. Analyses of the survey will include simple frequencies,
      percentages, and comparison of results related to HPV knowledge with sociodemographic and
      clinical information. Explorative analysis using parametric or non parametric statistics of
      the items included in the questionnaire and clinical forms will be done as appropriate. We
      will calculate the medical cost of patients enrolled in the study. The treatment protocols
      used for this protocol will reflect the current clinical practice accepted for the treatment
      of genital warts. We will use national reference cost list (SOAT) to determine the cost of
      procedures included.
    

Intervention name(s): Biopsy Excisional
Intervention type(s): Procedure
Intervention description(s): Biopsied from genital warts will be taken through an excisional procedure. Homeostasis will be obtained by using apply of Monsel solution. Biopsied sample will be splinted in order to obtain two pieces, one of them will be frozen (liquid Nitrogen) and designated to the HPV DNA PCR test (GP5, GP6) to be performed at the immunology lab at the National Cancer Institute in Bogotá. The other one will be kept in Formalin solution (10%) and sent to the pathology laboratory for haematoxiline - eosine paint and lecture.
Other intervention name(s): Resection genital warts
Observational model: Case-Only
Time perspective: Cross-Sectional
Sampling method: Non-Probability Sample
Study population: 
        Patients attending to gynecologic or STD consult in a mayor out patient clinic in Bogotá
        (PROFAMILIA).

        Female and male between 18 - 45 years of age living in Bogota, Colombia.
      
Biospecimen description: 
      Biopsies from genital warts will be taken through an excisional procedure. Homeostasis will
      be obtained by using topications of Monssell solution. Biopsy sample will be splinted in
      order to obtain two pieces, one of them will be frozen (liquid Nitrogen) and designated to
      the HPV DNA PCR test (GP5, GP6) to be performed at the immunology lab at the National Cancer
      Institute in Bogotá. The other one will be kept in Formalin solution (10%) and sent to the
      pathology laboratory for haematoxiline - eosine paint and lecture.
    
Keywords: Genital warts; HPV typing; HPV infections; Condyloma Acuminata