Evaluation of the Effect of Different Cleaning and Disinfection Procedures on the Involvement of Candida Species
Condition: Candidiasis, Oral
MeSH terms: Candidiasis; Candidiasis, Oral
Study type: Interventional

Summary: 
      Individuals may experience tooth loss as a result of trauma, periodontal destruction, lack of
      adequate bone support and clinically dental mobility. The increase in these tooth loss
      results in a condition called complete toothlessness in patients, and as a result of this,
      dentists treat complete dentures. It is known that the microorganism retention in the
      existing restorations is faster and higher than the natural tissues, and this leads to faster
      plaque build-up and hence bad odor. Candida species are common in oral flora, because they
      are numerous in the mouth and they are opportunistic pathogenic fungi that can make
      superficial and deep infections. The most important pathogen species among these parasites is
      Candida albicans. Mouth moniliasis or acute pseudomembrane candidiasis in the oral cavity of
      the so-called canker is caused by C.albicans. Chronic hyperplastic candidiasis is a clinical
      entity that is clinically inseparable from leukoplakia and completely separate from thrush.
      The accumulation of microorganisms in prosthetic materials is very important for the
      protection of the health of oral tissues and it is desirable to use appropriate cleaning and
      disinfection materials to minimize this accumulation.

      It is known in the literature that ozone and microwave technologies are used as disinfection
      technique. In this study, the application of ozone and microwave technologies in addition to
      the cleaning agents used as standard treatment will be compared in vivo. No such research has
      been found.The aim of this study was to evaluate the effect of different cleaning and
      disinfection procedures on the involvement of Candida species in complete denture patients.
    

Detailed description: 
      The study is planned to be performed on 72 patients between the ages of 18-70 who were
      diagnosed and followed up in the Department of Prosthodontics of the Faculty of Dentistry of
      Yuzuncu Yil University. Patients with complete disability and indication of total prosthesis
      are included in the case group. In addition, individuals with systemic disturbance of
      salivation, radiotherapy-chemotherapy patients who are known to be able to facilitate Candida
      growth will be excluded from our study. The complete prosthesis made of
      polymethylmethacrylate material will be delivered to the patients. Before delivery, the
      polished surfaces of the relevant prosthesis, its vertical size, whether the patient is
      suitable for use or not, and the delivery of the prostheses that have passed the relevant
      controls will be considered appropriate. Patients will be asked to come for follow-up at 1
      week and 1 month after the delivery of full dentures. The patients will be delivered with one
      of the glutaraldehyde, sodium hypochlorite or chlorhexidine digluconate solutions as the
      cleaning solution determined by the researchers during the initial delivery of their
      prostheses. Patients will be asked to clean with this relevant solution twice a day, in
      accordance with the specified times. At the 1 st and 1 st month follow-up, the prosthesis
      will be taken for the determination of the Candida number and species and the following
      procedures will be applied. Delivered prostheses will be taken in a 250 ml beaker (containing
      100 ml of distilled water). Then, with the help of a swab, it will be contacted to the inner
      surfaces and edges of the prosthesis to allow mixing into the distilled water in possible
      biofilm layers.

      Then, the prosthesis will be removed and the distilled water will be homogenized by mixing
      thoroughly with the help of a stirrer. Later Zhou et al. According to the method used; 1 ml
      of distilled water will be transferred to falcon tubes. Pre-cultural Falcon tubes will be
      kept on ice for up to 1 hour.

      Prior to culture, samples will be diluted with sterile distilled water (1: 1, 1:10, 1: 100).
      And be mixed well using a vortex mixer. Then take 0.5 ml of these diluted liquids to be
      planted in Sabouraud Dextrose Agar. After 48 hours of incubation (microscopic and macroscopic
      evaluation), the number of colony forming units (CFU) of distilled water will be counted
      using two researchers. Then, Candida lara will be identified by an API test which will
      include 19 carbohydrate assimilation tests and negative control.

      The same processes will be repeated after the disinfection process with ozone or microwave to
      compare the effectiveness of the disinfection process. Thus, the daily recommended by
      physicians for the daily cleaning process, the suppression of reproduction and reproduction
      of Candida species, and a new method proposed by physicians to be applied as a new method of
      ozone and microwave and how much efficiency of sterilization on the Candida number will be
      compared on the basis of the effects will be compared.

      Statistical analysis will be done and interpreted.
    

Intervention name(s): glutaraldehyde disinfection and microwave application; Chlorhexidine gluconate disinfection and microwave application; Sodium hypochlorite disinfection and microwave application; glutaraldehyde disinfection and ozone therapy; Chlorhexidine gluconate disinfection and ozone therapy; Sodium hypochlorite disinfection and ozone therapy
Intervention type(s): Other; Other; Other; Other; Other; Other
Intervention description(s): Combination of the chemical disinfection which could be done by patients and professional disinfection methods which is done by dentists; Combination of the chemical disinfection which could be done by patients and professional disinfection methods which is done by dentists; Combination of the chemical disinfection which could be done by patients and professional disinfection methods which is done by dentists; Combination of the chemical disinfection which could be done by patients and professional disinfection methods which is done by dentists; Combination of the chemical disinfection which could be done by patients and professional disinfection methods which is done by dentists; Combination of the chemical disinfection which could be done by patients and professional disinfection methods which is done by dentists
Intervention MeSH terms: Chlorhexidine; Chlorhexidine gluconate; Sodium Hypochlorite; Glutaral; Eusol
Allocation: Randomized
Intervention model: Parallel Assignment
Masking: None (Open Label)
Primary purpose: Prevention
Keywords: Prothesis stomatitis, disinfection methods