Salivary C1P and PGE2 Levels in Periodontitis Patients With T2DM and Effect of Non-Surgical Periodontal Treatment
Condition: Periodontitis; Type 2 Diabetes
MeSH terms: Periodontitis; Diabetes Mellitus, Type 2
Study type: Interventional

Summary: 
      Diabetes and periodontal disease are the most common chronic multifactorial and inflammatory
      diseases in humans, and there is a bidirectional relationship between type 2 diabetes and
      periodontitis. With the negative effects of the control of these two diseases, it results in
      an increase in the severity of diabetes and periodontitis, and they affect many systems
      together. To elucidate the role of ceramide, which is one of the possible biochemical
      mechanisms between diabetes and the degree of glycemic control and periodontitis, in
      inflammation. Aim of this study is the measurement of C1P and PGE2 in saliva and the effect
      of non-surgical periodontal treatment, which includes the degree of control of diabetes, oral
      hygiene education, tooth surface cleaning and root surface arrangement, on 3 months.

      A total of 102 subjects were included. Clinical periodontal measurements, saliva samples were
      collected from each individual at baseline and 3 months after non-surgical periodontal
      treatmet in periodontitis groups. Salivary C1P and PGE2 levels were determined by
      enzyme-linked immunosorbent assay (ELISA) method.
    


Intervention name(s): Non-surgical periodontal treatment; ELISA Test; Periodontal Examination; Saliva Sample Collection; HbA1c Level
Intervention type(s): Procedure; Diagnostic Test; Other; Other; Diagnostic Test
Intervention description(s): Routine non-surgical periodontal treatment will be applied to patients with periodontitis. The main aim of non-surgical periodontal treatment is to protect, heal and maintain the existing dentition. For this purpose, using manual, sonic or ultrasonic instruments, microbial dental plaque and calculus are removed by tooth surface cleaning and root surface straightening.; C1P and PGE2 levels in saliva samples obtained, and will be examined by ELISA test.; All diagnostic procedures were performed with examination instruments (periodontal probe, dental mirror, x-ray device, etc.). Periodontal clinical index measurements (PI, GI, BOP, PPD, CAL) recorded from all teeth. Routine panoramic radiographs were taken from all patients to determine alveolar bone resorption. Vertical/horizontal bone losses on the radiographs were evaluated and used in the diagnosis of periodontitis.; Saliva samples were collected from all subjects in the morning. Unstimulated saliva from each patient was collected by standard defined spitting method without stimulation.; Measurement of HbA1c levels from blood tests
Other intervention name(s): phase I periodontal therapy; scaling and root planing; full mouth debridement
Allocation: Randomized
Intervention model: Parallel Assignment
Intervention model description: A total of 102 (n=102) individuals were included in the study and were divided into 6 groups. The control groups of our study were Group 1 (HH), which included 17 both systemically healthy and periodontally healthy normoglycemic individuals, Group 2 (HP), which included 17 systemic healthy with Grade A periodontitis. Periodontally healthy T2DM patients were divided into two groups according to their HbA1c levels, and 17 patients with HbA1c levels below 7% were grouped as Group 3 (T2D+H), and 17 patients with HbA1c above 7% and 7% were grouped as Group 5 (T2D-H). 17 patients with T2DM with Grade B periodontitis with an HbA1c below 7% in the last three months were included in Group 4 (T2D+P), and 17 patients T2DM and periodontitis with an HbA1C value above 7% were included in Group 6 (T2D-P).
Masking: Double (Participant, Outcomes Assessor)
Primary purpose: Treatment
Keywords: saliva; prostaglandin e2; ceramide-1-phosphate; non-surgical periodontal treatment; phase I periodontal therapy; saliva biomarker; metabolic control; HbA1c; PGE2; C1P; periodontitis; type 2 diabetes mellitus