Nuclear Magnetic Resonance Spectroscopic Analysis of Urinary Metabolome in Sarcoidosis (RMN-SARCURINES)
Condition: Sarcoidosis
MeSH terms: Sarcoidosis
Study type: Observational

Summary: 
      "Sarcoidosis is a systemic granulomatous disease of unknown cause(s).Determining disease
      activity is a major element in the treatment decision.

      1H- Nuclear magnetic resonance (NMR) spectroscopy allows the identification of biomarkers in
      different pathologies and in particular in a pilot study of saliva of patients with
      sarcoidosis. Applications to sarcoidosis are still rare, having concerned only serum and
      saliva. In this context we hypothesize the existence of a difference in the metabolic
      products found in the urine of sarcoidosis patients with different degrees of activity and/or
      disease severity.

      We designed an analysis of urinary metabolomics in sarcoidosis patient using NMR spectroscopy
      with multivariate statistical analysis, followed by metabolite identification and pathway
      analysis. "
    

Detailed description: 
      "Sarcoidosis is a systemic granulomatous disease of unknown cause(s) with an almost constant
      lung involvement. Clinical expression varies greatly in both evolution and severity. The
      activity of the disease is currently based on serum markers (elevation of
      angiotensin-converting enzyme) with poor sensitivity/specificity or on imaging techniques
      (18F-FDG PET-CT) which are more invasive and more expensive. Determining disease activity is
      a major element in the treatment decision.

      The spectra of small molecules resulting from metabolism (metabolome/metabolomics) evaluated
      by spectrometry in different biological fluids (blood, saliva, urine) during different
      physiological or pathological conditions have been identified as promising biomarkers. 1H-
      Nuclear magnetic resonance (NMR) spectrometry allows the identification of biomarkers in
      different pathologies and in particular in a pilot study of saliva of patients with
      sarcoidosis. Applications to sarcoidosis are still rare having concerned only serum and
      saliva. The application of this method to the urinary metabolome has shown its effectiveness
      in other situations, for example in celiac disease.

      In this context we hypothesize the existence of a difference in the metabolic products found
      in the urine of sarcoidosis patients with different degrees of activity and/or disease
      severity.

      We designed an analysis of urinary metabolomics in sarcoidosis patient using NMR spectroscopy
      with multivariate statistical analysis, followed by metabolite identification and pathway
      analysis. "
    

Observational model: Cohort
Time perspective: Prospective
Sampling method: Non-Probability Sample
Study population: 
        Patients with a diagnosis of sarcoidosis according to ATS / ERS / WASOG criteria consulting
        at the Pulmonary Department of the Avicenne University Hospital
      
Biospecimen description: 
      Urinary sample
    
Keywords: Sarcoidosis; Urinary; NMR spectroscopy; Metabolomic