Translation Evaluation of Aging, Inflammation and HIV in Lung Function (TEAL)
Condition: HIV; COPD; Pulmonary Disease; Aging
MeSH terms: Inflammation
Study type: Observational

Summary: 
      Hypothesis;Aging modifies the risk of pulmonary dysfunction in HIV+ individuals.

      The study is a multicenter, prospective observational study of aging and pulmonary function
      in HIV. The investigator will determine the prevalence and risk factors for lung dysfunction
      as quantified by pulmonary function testing in both younger (<50 years) and older (≥50 years)
      HIV+ and HIV-uninfected controls. The investigator will build on existing cohorts and enrich
      enrollment for individuals over the age of 50 while adjusting for important co-variates such
      as ART, smoking history, co-infections, and illicit drug use. Evaluations will be scheduled
      at baseline, 18 months, and 36 months. Study visits will consist of blood draw,
      questionnaires, and pulmonary function testing.
    

Detailed description: 
      All clinical procedures will be performed by a registered nurse. Bronchoscopy procedures will
      be performed by physician.

      Subjects will be instructed to avoid smoking, alcoholic beverages and caffeine or medications
      containing caffeine for eight hours, prior to the visit. In addition, the subject will be
      asked to hold all medications that are bronchodilators prior to the test being administered
      and to eat a light meal 2-4 hours prior to testing.

      Part I

      Respiratory questionnaires:

      Translation Evaluation of Aging, Inflammation and HIV in Lung Function questionnaire, MMRC
      and the ST. George's Questionnaire will be administered. These are paper and pencil
      questionnaires that inquire about respiratory symptoms, quality of life, smoking history and
      other health issures. This will take approximately 30 minutes to complete.

      Blood Sample collection:

      The subject will be asked to provide a blood sample by venipuncture of approximately 40mls (8
      teaspoons) at this study visit. The purpose of this collection is to have blood processed for
      serum, plasma, and PBMCS, and a protion stored in RNAlater to be used for future use. A
      hemoglobin and Carboxyhemoglobin will be done in order to calculate the DLCO. The
      de-identified samples will be processed and stored at -80C.

      Personal genetic information will not be provided to individual research subjects as Dr.
      Morris's laboratory is not CLIA certified and because the blood samples will be
      de-identified.

      Lung function testing:

      The routine lung function endpoints of FVC, FEV1, FEV1/FVC, and FEF25-75% will be measured
      with the flow-volume loop recorder on a NDD EasyOne Pro testing system before and after
      bronchodilator administration. The system is calibrated for body temperature and pressure of
      saturated gas and volumes, per American Thoracic Society (ATS) standards . The best of three
      reproducible forced expiratory attempts is used in analysis. Percent predicted spirometric
      values are based on age, height, gender, and ethnicity. DLco will be measured using the
      automated single-breath procedure of the integrated testing system, which conforms with ATS
      standards. All testing will be reviewed by (PI). If any concerns or abnormal results are
      discovered during this testing, a member of the research team will notify the subject or the
      subject's primary care physician, who will assess the need to provide additional evaluation.

      Subjects will have these tests done at baseline, 18 months and 36 months. Each visit will
      last 3 to 4 hours. The visits will take place in the MUH 6th floor CTRC

      6 minute walk test; Particiapant will rest for 10 minutes prior to beginning the test. BP and
      pulse ox are checked prior to the test. The six-minute walk tests are performed in a 100-foot
      segment of straight hallway marked at 1-foot intervals. In addition to the usual ATS
      protocol, the patient is monitored with wireless pulse oximetry and the time and distance
      recorded at which they desaturate to <88% or at 6 minutes. The test is also terminated if the
      saturation falls below 80%. Dyspnea and perceived exertion are rated using the Borg scale at
      the end of test. BP is checked post test. For this study the test will be performed off of
      supplemental oxygen if possible. O2 can be used on subjects who use O2 regularly to keep
      O2Sat > 88%

      Oral specimen collection. The investigator will collect a 10ml oral wash, a tongue scraping
      and a 1 to 5ml saliva collection. This is a one time collection at any visit.

      Part II Bronchoscopy visit (Selected subjects only) Subjects will be called back depending on
      the outcome of the PFT's and medical history. Subjects selected for bronchoscopy may have
      lung testing repeated depending on the time lapse between PFT and bronchoscopy. This will be
      decided at the discreation of the PI There will be two visits for the bronchoscopy
      participants. Both visits will take place in MUH 6th floor CTRC

      Visit One: Apprx. 3 hours Screening procedures Oral Wash Tongue scraping Sputum induction
      Blood collection (Repeat breathing test will be completed at the discretion of the principal
      investigator)

      Oral Wash The participant will gargle with .9% saline for 1 minute and then spit into a
      container to provide a sample of approximately 10mls (2 teaspoons) at this study visit.

      Tongue Scraping A tongue blade or similar object will be used to scrape the tongue. This
      procedure should only take minutes.

      Sputum Induction The subject will breathe in and out mists of saline (3%-5% saline) with a
      mouthpiece for 20 minutes. During this time, they will actively cough and spit out saliva and
      sputum into two separate cups every 4 minutes. We will frequently measure lung function
      during the procedure (every 4 min) using a peak flow meter.

      Blood The investigator will take a blood sample of approximately 60mls (10 teaspoons) at this
      study visit for research blood. (serum, plasma, and PBMCS ) In addition, part of this blood
      will be used to perform routine clinical blood tests to assure that it is safe to proceed
      with the bronchoscopy test. (CBC/DIFF/PLT, LYTS, BUN, Creatinine, Glucose, PT/PTT)

      Visit Two: Approx. 6 hours Oral wash Tongue scraping with rinse Bronchoscopy Monitoring
      (Blood draw- if visit two is more than 24 hours from visit one to repeat clinical labs)

      Oral wash and Tongue Scraping will be repeated, and then the subject will rinse with 10cc's
      of Listerine. to assure that there are no bacteria left behind in the mouth prior to the
      bronchoscopy.

      Bronchoscopy: Fiberoptic bronchoscopy will be performed using the ATS recommendation and
      institutional protocols for conscious sedation. The testing will be performed by a physician
      certified in pulmonary medicine and proficient in the procedure and certified by UPMC.
      Subjects will be instructed to fast overnight and withhold aspirin and anti-inflammatory
      analgesics for 48 hours prior to the procedure. Subjects will have undergone a pre-
      bronchoscopy spirometry and only clinically stable subjects (FEV1>30% predicted after
      albuterol) will undergo the procedure. Subjects will also recieve Proventil HFA Inhaler 2
      puffs, after 5 minutes will recieve a second dose of Proventil 2 puffs prior to sending
      patient to the Bronchoscopy suite. Subjects will recieve an intramuscular injection of a mild
      sedative and a drug to dry secretions Demerol 50mg IV and Atropine 0.6mg.

      Subjects will receive a local anesthetic to the upper and lower airways and an intravenous
      sedative and/or analgesic (midazolam; fentanyl). The midazolam dose will not exceed 10mg
      and/or the fentanyl dose will not exceed 200 micrograms. Lidocaine will not exceed a dose of
      9 mg/kg (0.9 cc of 1%/kg) or a maximum dose of 600 mg (60 cc 1%) during the procedure.
      Subject monitoring will follow the local protocol for conscious/deep sedation. If needed,
      subjects will receive additional short acting beta agonist. After transoral insertion of the
      bronchoscope a general inspection of the airways will be performed followed by the
      bronchoalveolar lavage. Blood pressure, heart rate, and oxygen saturation will be monitored
      throughout the procedure.

      Bronchoalveolar lavage:

      BAL will be performed with room temperature normal saline. After wedging the bronchoscope in
      a (sub)segmental bronchus, approximately 50cc of saline will be instilled with each washing
      and withdrawn by syringe aspiration. This will be repeated up to 4 times per segment as
      needed. Amount of wash and selection of segments will be at the discretion of the physician.
      Many clinical bronchoscopies are within this range of volume, and research bronchoscopies
      have been found to be safe with volumes ranging from 100 to 300 cc even in subjects with
      severe asthma (Wenzel S et al. Bronchoscopic evaluation of severe asthma. American Journal of
      Respiratory and Critical Care Medicine 1997;156:737-43; Summary and recommendations of a
      workshop on the investigative use of fiberoptic bronchoscopy and bronchoalveolar lavage in
      asthmatic patients. Chest 1985;88:136-38). We will stop the lavage at any point where we do
      not feel that it would be clinically tolerated by the patient.

      The volume collected after each withdrawal will be recorded.

      Bronchial brushing:

      Cytology bronchial brushing involves positioning the bronchoscope at the right middle or
      lower lobe, passing a cytology brush through the bronchoscope and gently brushing the lining
      of the airways. This may be repeated at least eight more times per segment to ensure an
      adequate sample collection. If any sample collected is not acceptable or adequate for
      analysis, another sample may be collected so long as the physician performnig the
      bronchoscopy procedure decides that no significant risk is present with the additional
      collection.

        1. Participant will be monitored at UPMC Montefiore until the sedation has worn off. After
           the bronchoscopy is completed, the subject will wait in the bronchoscopy recovery area
           or the clinical research CTRC to make sure thier breathing has not been affected by the
           procedure and to be sure that the anesthesia has worn off. Pulse oximetry montioring
           will continue throughout recovery period. If the subject is not able to breathe as well
           as they could before the bronchoscopy, we will give additional albuterol and check the
           breathing again.Participant will not be allowed to leave the recovery area until their
           breathing is back to their normal level. If a participants breathing does not return to
           baseline they will be required to be admitted overnight

        2. Participant will not be allowed to drive themselves home after the bronchoscopy.

      Broncoscopy pre and post sedation care will be conducted in the MUH CTRC. Staff are trained
      research registered nurses and familiar with post bronchoscopy monitoring. Pulse oximetry
      will be closely monitored and the subect will not be discharged until anethseia has worn off
      and the subject's breathing is back to normal.

      The bronchoscopy procedure will take about 45 minutes, the participant will not be discharged
      until anesthesia has worn off and their breathing is back to normal.

      All bronchoscopies will be done in the pulmonary division bronchoscopy suite. Resuscitative
      equipment and personnel are present in the room to minimize risk. In addition, all research
      bronchoscopies will have an RN present, as well as the physician in charge of the procedure.
      The physician will be a faculty member of UPMC with brochoscopy privileges.

      Bronchoscopy will only be offered once.
    

Intervention name(s): PFT; lab work; 6MWT; questionnaires
Intervention type(s): Procedure; Procedure; Procedure; Other
Intervention description(s): Lung function endpoints of FVC, FEV1, FEV1/FVC, and FEF25-75% will be measured with the flow-volume loop recorder on a NDD EasyOne Pro system before and after bronchodilator administration.; The blood will be processed for serum, plasma, and PBMCS, and a portion stored to be used for future use. A hemoglobin and Carboxyhemoglobin will be done in order to calculate the DLCO.; The six-minute walk tests are performed in a 100-foot segment of straight hallway marked at 1-foot intervals.; TEAL, MMRC and ST. George's Questionnaire will be administered. They are paper questionnaires that inquire about respiratory symptoms, quality of life, smoking history and other health issues
Other intervention name(s): Spirometry; blood work; six minute walk test; survey
Observational model: Cohort
Time perspective: Prospective
Sampling method: Probability Sample
Study population: 
        Participants will be subjects in the Pitt Men's Study or the PACT clinic.
      
Biospecimen description: 
      Saliva, blood, bronchial wash
    
Keywords: HIV; lung; aging