Profile Of Methylation and Gene Expression Of Gene ADRB3 And Effects Of Folate Intake
Condition: Overweight and Obesity
MeSH terms: Overweight
Study type: Interventional
Phase: Phase 2

Summary: 
      ABSTRACT

      Epigenetic mechanisms may be involved in the regulation of lipid metabolism and inflammatory
      markers may therefore contribute to the cardiovascular risk profile, as well as environmental
      factors have a significant impact on the epigenetic program of gene expression. Methyl groups
      are usually added or removed as needed and can be influenced by diet soon, dietary factors
      have been linked to the change of DNA methylation, resulting in changes in gene expression.
      Research on the prevalence of gene methylation levels on the impact of nutrient intake, as
      well as the inflammatory condition and the results of dietary interventions for the genes, it
      is a sparse area in studies and, therefore, expanding of the knowledge. Thus, understanding
      the effects of a diet rich in antioxidants and hazelnut oil, in inflammation and DNA
      methylation profile is important because chronic diseases with cardiovascular disease can
      begin with the consumption of modified lipids and can research this stage provide important
      dietary allowances for the development of this disease prevention strategies even in
      adulthood. This PhD project is linked to a larger project entitled "II Diagnosis Cycle and
      Intervention Food Situation, Nutrition and Noncommunicable Diseases Prevalent over the City
      Population of João Pessoa / PB" (II DISANDNT / PB) and have two methodological designs: the
      first one is the composition of a representative sample of adults in the city of João Pessoa,
      from II DISANDNT / JP, population-based study and cross-sectional. The second methodological
      design was developed with a subsample of the above population, selected from criteria being
      the type and interventional clinical trial. This model will have to evaluate the effect of a
      diet rich in antioxidant and hazelnut oil, in inflammation and gene expression.
    

Detailed description: 
      This thesis is linked to a population-based survey entitled "Diagnosis II Cycle and
      Intervention Food Situation, Nutrition and Noncommunicable Diseases Prevalent over the city
      of João Pessoa Population / PB" (II DISANDNT / PB).

      For realization of this thesis, a first population-based study, representative of adults in
      the east areas and west of the city of João Pessoa, there was the calculation of a
      representative sample for the age group of adults, using information provided by the city, as
      map the municipality, the number of blocks per district and the Brazilian Institute of
      Geography and Statistics.

      Home visits and implementation of the survey questionnaires were carried out by teams of
      undergraduate researchers from the Nutrition courses, masters and doctoral students of the
      Post Graduate Program in Nutrition Sciences (PPGCN) UFPB duly previously trained at the
      beginning of data collection after the pilot study and following.

      The trained teams, after recognizing the drawn court, were instructed to select all
      households in the court. The blocks that did not have homes, the homes in which those
      responsible have not accepted to participate and the amount of individuals who refused to
      participate in randomly selected households were computed and resorteadas randomly thereafter
      to minimize losses.

      In each residence drawn all individuals, adults aged between 20-59 years were invited to
      participate in the research through application of socioeconomic and demographic
      characteristics questionnaires, epidemiological characterization, nutritional assessment and
      food intake, biochemical evaluation and brands epigenetic.
    

Intervention name(s): Folate and capsule of oil hazel; Folate and placebo; antioxidant and placebo
Intervention type(s): Dietary Supplement; Dietary Supplement; Dietary Supplement
Intervention description(s): rich diet aministracao folate and capsule of oil hazel; rich diet aministracao folate and placebo; antioxidant diet (90μg folate) and placebo capsule
Intervention MeSH terms: Folic Acid; Vitamin B Complex; Antioxidants
Allocation: Randomized
Intervention model: Factorial Assignment
Masking: Single (Investigator)
Primary purpose: Prevention