Effects of Recurrent Application of Liquid-PRF on Hard and Soft Tissues
Condition: Liquid-PRF; A-PRF Plus
Study type: Interventional

Summary: 
      Procedure: Tooth is extracted, then A-PRF+ is placed inside the socket. After that the ridge
      dimensions is asses clinically at the baseline) in addition to clinical assessment of the
      gingival biotype at the baseline.

      Then patients will be divided into 2 groups:

      Group 1: 50% of patients will have recurrent Liquid-PRF injections inside the extraction
      socket every 2 weeks (A-PRF+, followed by recurrent Liquid-PRF) Group 2: 50% of patients
      without recurrent (just A-PRF+) After 3 months from extraction: both groups will be assessed
      clinically (gingival biotype) & (Ridge dimensions) in order to assess the role of recurrent
      application of Liquid-PRF on these parameters.
    

Detailed description: 
      In this study, we will select 20 patients who need a simple tooth extraction, then determine
      the gingival biotype, take a blood sample to prepare A-PRF+ (centrifugation of the patient's
      blood for 8 min at 1300 rpm using red caps tubes), then do the extraction. After that, A-PRF+
      is prepared and inserted into the extraction socket, and sutured using horizontal mattress
      suturing technique.

      After the clinical assessment of the ridge dimensions at the baseline

      Then patients will be divided into 2 groups:

      Group 1: 10 patients will have recurrent Liquid-PRF injections inside the extraction socket
      every 2 weeks (A-PRF+, followed by recurrent Liquid-PRF) Liquid-PRF preparation:
      Centrifugation of the patient's blood for 8 min at 2500 rpm using white caps tubes.

      Group 2: 10 patients without recurrent application of Liquid-PRF (just A-PRF+) After 3 months
      from extraction: both groups will be assessed clinically (gingival biotype) & (Ridge
      dimensions) in order to evaluate the effect of recurrent application of Liquid-PRF on these
      parameters.

      Assessment method:

      for Hard tissue evaluation (Bone): we assess the dimensions clinically For soft tissue: we
      will use a periodontal probe to assess the gingival thickness after giving local anesthesia.

      ---------------------------------------------

      Selection Criteria

      INCLUSION CRITERIA:

        1. Men and women over 18 years of age

        2. Indication of a simple extraction

        3. Delivery of signed informed consent

      EXCLUSION CRITERIA:

        1. Need for multiple extractions

        2. Presence of severe swelling prior to surgery in the extraction area

        3. Regular treatment with non-steroidal anti-inflammatory drugs (NSAIDs) or other
           anti-inflammatory drugs

        4. Presence of hematologic disease

        5. Previous radiation, chemotherapy, or immunosuppressive treatments

        6. Uncontrolled diabetes mellitus, metabolic bone disease, ongoing treatment with
           bisphosphonates drugs, or pregnancy
    

Intervention name(s): Liquid platelet-rich fibrin; Advanced Platelet-rich fibrin plus
Intervention type(s): Other; Other
Intervention description(s): Liquid platelet-rich fibrin is prepared from the patient's blood and injected into the extraction socket every two weeks (up to 3 months = Just 4 times of application); A-PRF+ is placed into the extraction socket immediately after tooth extraction
Other intervention name(s): Liquid-PRF
Allocation: Randomized
Intervention model: Parallel Assignment
Intervention model description: Human patients:
20 patients who need tooth/ teeth extraction would be selected according to the following criteria:
INCLUSION CRITERIA:
Men and women over 18 years of age
Indication of a simple extraction
Delivery of signed informed consent
EXCLUSION CRITERIA:
Need for multiple extractions
Presence of severe swelling prior to surgery in the extraction area
Regular treatment with non-steroidal anti-inflammatory drugs (NSAIDs) or other anti-inflammatory drugs
Presence of hematologic disease
Previous radiation, chemotherapy, or immunosuppressive treatments
Uncontrolled diabetes mellitus, metabolic bone disease, ongoing treatment with bisphosphonates drugs, or pregnancy
Masking: None (Open Label)
Primary purpose: Treatment
Keywords: recurrent; Liquid platelet-rich fibrin; Advanced platelet-rich fibrin plus; ridge preservation