Gingival Crevicular Fluid Bone Morphogenetic Protein - 2 Release Profile Following the Use of Perforated Membrane
Condition: Periodontal Pocket
MeSH terms: Periodontal Pocket
Study type: Observational [Patient Registry]

Summary: 
      One of the most important elements suggested to be provided through membrane perforations to
      augment regeneration are cell released growth factors from the overlying periosteal and
      gingival cells. A direct correlation could exist between the number of such cells and their
      available released growth factors. To test this assumption, this study was designed to
      evaluate levels of bone morphogenetic protein-2 in gingival crevicular fluid (GCF) during the
      early stages of healing for sites treated by MPM compared to that of the occlusive barrier
      membranes.
    

Detailed description: 
      The subjects were recruited consecutively from the list of patients seeking care for
      periodontal problems at the Department of Periodontology of the Faculty of Dental Medicine,
      Al Azhar University, Cairo, Egypt, between March 2012 to December 2012. The criteria
      implemented for patient inclusion were: 1) no systemic diseases which could influence the
      outcome of the therapy; 2) good compliance with the plaque control instructions following
      initial therapy; 3) teeth involved were all vital with score 0 mobility ; 4) each subject
      contributed matched pairs of two or three walled intrabony interproximal defects in premolar
      or molar teeth 5) the selected 2- or 3-wall intrabony defect depth ranged from 3-5 mm as
      detected in diagnostic periapical radiographs; 6) selected pocket depth (PD) ≥ 6 mm and
      clinical attachment level (CAL) ≥ 5 mm at the site of endosseous defect four weeks following
      initial cause-related therapy 7) endosseous radiographic defect angle ≤55 and ≥15 8)
      availability for the follow-up and maintenance program; 9) absence of periodontal treatment
      for the previous year; 10) absence of systemic medication or antibiotic treatment for the
      previous 6 months; 11) absence of a smoking habit and 12) absence of occlusal interferences.
      Pregnant females were excluded from participating in the study. Patients were also excluded
      from the study if they presented with opened interproximal contact, or inadequate compliance
      with the oral hygiene maintenance schedule. The experimental protocol was approved by the
      Ethical Committee of El Azhar University (OMD - 45 - 2012, clinical trial registration
      number: Al Azhar University 22-31). Research procedures were explained to all patients and
      they agreed to participate in the study and signed the appropriate informed consent form of
      Al Azhar University.
    

Intervention name(s): perforated membrane; G2
Intervention type(s): Procedure; Procedure
Intervention description(s): perforated membrane that could give a chance for posetive contributionn of periosteal and gingival mesenchymal stem cells in periodontal regeneration; tradetional occlusive collagen membrane
Other intervention name(s): collagen membranes; guided tissue membranes
Observational model: Cohort
Time perspective: Prospective
Sampling method: Probability Sample
Study population: 
        Fifteen non-smoking patients (8 males and 7 females) who were 31 to 51 years of age at the
        time of baseline examination (mean age 33.8 ± 6.1) with severe chronic periodontitis
        (Armitage 1999) participated in this prospective, randomized and blinded clinical trial.
        The subjects were recruited consecutively from the list of patients seeking care for
        periodontal problems at the Department of Periodontology of the Faculty of Dental Medicine,
        Al Azhar University, Cairo, Egypt, between March 2012 to December 2012. The experimental
        protocol was approved by the Ethical Committee of El Azhar University (OMD - 45 - 2012).
        Research procedures were explained to all patients and they agreed to participate in the
        study and signed the appropriate informed consent form of Al Azhar University.
      
Keywords: growth factors; periodontal pockets