Use of MCT Oil for Enhancement of Weight Loss in Obese Patients
Condition: Obesity; Weight Loss
MeSH terms: Body Weight; Weight Loss
Study type: Interventional

Summary: 
      Twelve obese patients approved for gastric bypass surgery will be randomized to either
      receive an MCT-based or LCT-based liquid diet for a 4 week period. These diets will be
      identical except for the quality of the fat. The intervention will be double-blinded. Six
      patients will receive a daily diet consisting of 5 liquid meals using the HMR 800 meal
      replacement product (Health Management Resources, Inc; 160 calories/shake) with 44.5 grams
      MCT oil (Life Enhancement Products, Inc., Petaluna, CA; 8.3 kcal/gm) added. Six patients will
      receive the same HMR diet but with 41 grams LCT oil (corn oil; 9 kcal/gm) added. Patients in
      both groups will be given a list of supplemental foods that are suitable for the study. They
      may choose to eat up to an additional 700 kcal per day from this list and will be asked to
      complete food records for monitoring of their caloric intake. Patients will undergo gastric
      bypass surgery after 4 weeks of being on their diets. Patients in both groups will be seen
      weekly by a registered dietitian and a physician. Blood pressure and weight will be recorded,
      and any adverse events will be noted and cared for as is appropriate. Dietary instruction
      will be reviewed as needed.Both diets will be 1170-1870 kcal/day, consisting of 36% CHO,
      26.2% protein, and 37.6% fat. The MCT diet will contain 30.2% of total calories at MCT oil.
      The percentages given are based on the shake and oil consumption only (1170 kcal/day). The
      remainder of the diet will vary according to patients' selections from the list of permitted
      foods, up to an additional 700 kcal/day (for the total 1870 kcal/day). All subjects will
      receive a multi-vitamin each day. Subjects will also receive two Fibercon capsules per day to
      prevent possible bowel changes associated with being on a full liquid diet. During surgery,
      biopsies of visceral and omental adipose tissue, as well as subcutaneous abdominal adipose
      tissue, will be obtained. Tissue will be analyzed to determine mRNA levels of key enzymes in
      fatty acid esterification, lipolysis, and oxidative disposition. This preliminary phase will
      be conducted to determine whether visceral, omental, and subcutaneous abdominal adipose
      tissue behave similarly when exposed to an MCT-based diet.
    

Detailed description: 
      In this the preliminary phase, a total of 12 obese patients who have been accepted to undergo
      gastric restrictive surgery will be recruited. Although the inclusion criteria for the
      secondary companion pilot study include diabetic patients, having diabetes mellitus is not
      necessary for the testing of the hypothesis in the preliminary phase. The aim is to test
      whether subcutaneous adipose tissue in the abdominal region is similar to visceral and
      omental adipose tissue in terms of cellular mechanisms after ingestion of MCT oil.

      Twelve obese patients approved for gastric bypass surgery will be randomized to either
      receive an MCT-based or LCT-based liquid diet for a 4 week period. These diets will be
      identical except for the quality of the fat. The intervention will be double-blinded. Six
      patients will receive a daily diet consisting of 5 liquid meals using the HMR 800 meal
      replacement product (Health Management Resources, Inc; 160 calories/shake) with 44.5 grams
      MCT oil (Life Enhancement Products, Inc., Petaluna, CA; 8.3 kcal/gm) added. Six patients will
      receive the same HMR diet but with 41 grams LCT oil (corn oil; 9 kcal/gm) added. Patients in
      both groups will be given a list of supplemental foods that are suitable for the study. They
      may choose to eat up to an additional 700 kcal per day from this list and will be asked to
      complete food records for monitoring of their caloric intake. Patients will undergo gastric
      bypass surgery after 4 weeks of being on their diets. Patients in both groups will be seen
      weekly by a registered dietitian and a physician. Blood pressure and weight will be recorded,
      and any adverse events will be noted and cared for as is appropriate. Dietary instruction
      will be reviewed as needed.

      Both diets will be 1170-1870 kcal/day, consisting of 36% CHO, 26.2% protein, and 37.6% fat.
      The MCT diet will contain 30.2% of total calories at MCT oil. The percentages given are based
      on the shake and oil consumption only (1170 kcal/day). The remainder of the diet will vary
      according to patients' selections from the list of permitted foods, up to an additional 700
      kcal/day (for the total 1870 kcal/day). Subjects will receive a multi-vitamin each day.
      Subjects will also receive two Fibercon capsules per day to prevent possible bowel changes
      associated with being on a full liquid diet. It is estimated patients will lose between 10
      and 30 pounds before surgery. This is a standard of care as all patients are encouraged to
      lose this amount prior to surgery. This small amount of weight loss from initial body weight
      is considered beneficial in reducing complications of gastric bypass surgery in morbidly
      obese patients with significant co-morbidities.

      During surgery, biopsies of visceral and omental adipose tissue, as well as subcutaneous
      abdominal adipose tissue, will be obtained. Tissue will be analyzed to determine mRNA levels
      of key enzymes in fatty acid esterification, lipolysis, and oxidative disposition. This
      preliminary phase will be conducted to determine whether visceral, omental, and subcutaneous
      abdominal adipose tissue behave similarly when exposed to an MCT-based dietIn Phase 1 and 2,
      these results will be used to extrapolate effects on visceral and omental adipose tissue from
      effects seen on subcutaneous adipose tissue.

      In addition, small samples of the tissue (approximately .25 cm x .25cm) for each of the 12
      subjects will be shipped to Quantomix, where the samples will be viewed under electron
      microscopy. There will be no additional tissue removed in the OR for this, and there will be
      no added risk to the subjects. Quantomix will not receive any identifying information on the
      samples; therefore there will be no breach of confidentiality.

      STUDY PROCEDURES: Screening for Preliminary Phase Twelve obese subjects will be recruited
      from the Nutrition and Weight Management Center population of gastric restrictive surgery
      patients. The subjects will have met all requirements for and received prior approval for the
      gastric restrictive surgery. The patient will sign the IRB-approved Informed Consent -
      Surgical Intervention form at the Baseline visit. Inclusion criteria includes age 18-65 years
      and BMI >40 kg/m2 or >35 kg/m2 with co-morbidities. Exclusions are those that are a part of
      the normal standard of care in the pre-selection process for approval of surgery, clinically
      severe lactose intolerance, alcohol dependence, and a significant egg allergy.

      Baseline and Remaining Visits The Baseline visit will be within two weeks of the screening
      visit. For the procedures to be performed at the baseline and weekly visits, see Table A
      attached.

      Procedure for subcutaneous adipose tissue needle biopsy The surgeon will remove three pieces
      of fat tissue measuring 2 inches x 2 inches x 2 inches from the fat under the skin during the
      gastric restrictive surgery. In the surgical field, it is appropriate to remove this large of
      a piece of tissue in order to be able to extrapolate and define how much tissue will be
      required to be removed by punch biopsy in Phase 1 and 2 of the secondary companion study (in
      the secondary companion study, it is expected that 100 mg subcutaneous tissue will yield 3
      micrograms of total RNA. The amount of tissue removed during surgery will be greater than
      that amount to ensure the projected yield). The surgeon will also remove two pieces of fat
      tissue from the abdomen during surgery. In total, one piece will be taken from the fat tissue
      covering the stomach (omental), one piece from just under the skin (subcutaneous), and the
      other piece from the fat tissue covering the bowels or intestines (visceral). These are fat
      tissues that are in the area that the surgery will be performed. The biopsy will not
      interfere in any way with the surgery. The surgeon will also draw a sample of blood (12 mL)
      to be used for hematology, serum chemistry, hemoglobin A1C, serum lipids, thyroid function
      test, serum insulin, serum hydroxybutyrate (possibly elevated in MCT-fed subjects), serum
      leptin, and serum free fatty acid concentration. The surgeon will explain all of this to the
      subject prior to surgery.

      Study schedule:

      Pre-Op Diet:

        1. Prior to the gastric restrictive surgery, eligible patients will receive dietary
           instructions by a registered dietitian for a daily diet consisting of 5 liquid meals
           using the HMR 800 meal replacement product (Health Management Resources, Inc.) with 44.5
           grams MCT oil or 41 grams LCT oil added. Patients in both groups will be given a list of
           supplemental foods that are suitable for the study. They may choose to eat up to an
           additional 700 kcal per day from this list and will be asked to complete food records
           for monitoring of their caloric intake. Depending on how much food each patient eats,
           this will be a daily caloric intake of approximately 1170-1870 calories. All patients
           will be instructed to refrain from any alcohol intake while in the study.

        2. All patients will undergo gastric bypass surgery after 4 weeks of MCT oil/HMR or LCT
           oil/HMR.

      Post-Op Diet:

      Following surgery, subjects will follow the usual standard of care implemented in the
      Nutrition and Weight Management Clinic through post-operative visits with the surgeon and
      registered dietitian. Diet Stages Following Gastric Restrictive Surgery is attached hereto as
      Appendix A.

      Biopsy Procedure:

      1.During gastric restrictive surgery, biopsies of visceral and omental adipose tissue as well
      as subcutaneous abdominal adipose tissue will be obtained.

      The surgeon will remove three pieces of fat tissue measuring 2 inches x 2 inches x 2 inches
      from the fat under the skin during the gastric restrictive surgery. In the surgical field, it
      is appropriate to remove this large of a piece of tissue in order to be able to extrapolate
      and define how much tissue will be required to be removed by punch biopsy in Phase 1 and 2 of
      the secondary companion study (In the secondary companion study, it is expected that 100 mg
      subcutaneous tissue will yield 3 micrograms of total RNA. The amount of tissue removed during
      surgery will be greater than that amount to ensure the projected yield). The surgeon will
      also remove two pieces of fat tissue from the abdomen during surgery. In total, one piece
      will be taken from the fat tissue covering the stomach (omental), one piece from just under
      the skin (subcutaneous), and the other piece from the fat tissue covering the bowels or
      intestines (visceral). These are fat tissues that are in the area that the surgery will be
      performed. The biopsy will not interfere in any way with the surgery. The surgeon will also
      draw a sample of blood to be used for hematology, serum chemistry, hemoglobin A1C, serum
      lipids, thyroid function test, serum insulin, serum hydroxybutyrate (possibly elevated in
      MCT-fed subjects), serum leptin, and serum free fatty acid concentration. The surgeon will
      explain all of this to the subject prior to surgery.

      Screening period (up to 2 weeks)

        1. Prior to the Screening visit, in the pre-operative visit, the Preliminary Phase will be
           explained to patients who have been pre-approved for gastric restrictive surgery.
           Questions will be answered at that time.

        2. Initial screening form will be completed

        3. Pre-approval for gastric restrictive surgery will be documented.

        4. IRB-approved written consent form, signed at the pre-operative visit with the surgeon
           co-investigator and medical co-investigator will be documented.

        5. Patient will be scheduled to return for Baseline visit if eligible for study.

      Baseline (within 2 weeks of screening) ± 4 days. Beginning of the study will be within 2-3
      weeks after screening visit.

        1. Fasting blood samples will be obtained for hematology, serum chemistry, hemoglobin A1C,
           serum lipids, thyroid function test, serum insulin, serum hydroxybutyrate (possibly
           elevated in MCT-fed subjects), serum leptin, and serum free fatty acid concentration.

        2. Weight and blood pressure will be recorded.

        3. Study diet and instructions will be dispensed.

        4. Patient will be scheduled for surgery at the end of Week 4 of the study diet.

      Weekly visits (with registered dietitian and physician)

        1. Weight and blood pressure will be recorded.

        2. Dietary instruction will be reviewed as necessary.

        3. Adverse effects will be noted.

        4. Study diet will be dispensed as needed.

        5. At the visit with the dietitian and physician one day before the gastric bypass surgery,
           blood samples will again be obtained for hematology, serum chemistry, hemoglobin A1C,
           serum lipids, thyroid function test, serum insulin, serum hydroxybutyrate (possibly
           elevated in MCT-fed subjects), serum leptin, and serum free fatty acid concentration.

      Unscheduled visits (UV):

      If necessary for patient safety, unscheduled visits may be arranged. Unscheduled source
      document sets used for visits attended between routine patient visits will be labeled with
      the last attended visit number followed by a decimal point and the number of the unscheduled
      visit (i.e. if two unscheduled visits are attended between Week 0 and Week 1 then those
      unscheduled visits would be labeled as unscheduled visit sets 0.1 and 0.2 in chronological
      order).

      At unscheduled visits the following shall be completed as indicated:

        -  Blood samples will be collected for laboratory evaluation, if clinically indicated.

        -  Adverse events will be documented.

        -  A physical examination will be performed, if clinically indicated.

        -  An ECG will be performed, if clinically indicated.
    

Intervention name(s): 1170-1870 kcal/day liquid diet; Gastric bypass surgery; Biopsy.visceral adipose tissue; Biopsy.omental adipose tissue; Biopsy. subcutaneous abdominal adipose tissue; Blood sample
Intervention type(s): Behavioral; Procedure; Procedure; Procedure; Procedure; Procedure
Allocation: Randomized
Intervention model: Parallel Assignment
Masking: Double
Primary purpose: Treatment
Keywords: Hypocaloric MCT oil liquid diet; Hypocaloric LCT oil liquid diet; Visceral and omental adipose tissue; Subcutaneous abdominal adipose tissue; Gastric bypass surgery