Adiponectin, ICAM-1, VCAM-1 Levels and Metabolic Syndrome in Obese Adolescents
Condition: Obesity; Abdominal Obesity; Metabolic Syndrome; Hypertension; Hypertriglyceridemia; HDL Cholesterol, Low Serum; Hyperglycemia
MeSH terms: Hypoalphalipoproteinemias; Obesity; Metabolic Syndrome; Hyperglycemia; Hypertriglyceridemia; Obesity, Abdominal; Syndrome
Study type: Observational [Patient Registry]

Summary: 
      Adipose tissue secreting a number of adipokines which regulate insulin sensitivity, energy
      metabolism and vascular homeostasis, so the dysfunction of adipose tissue is linked with the
      incidence of obesity accompanied with insulin resistance, hypertension and cardiovascular
      disease (1). Obesity is known to alters the expression of adipokines due to the adipose
      tissue hypertrophy (2), including adiponectin, in which able to exert a potent
      anti-inflammatory and vascular protective effect (2).

      It has been proposed that adiponectin acts to prevent the vascular dysfunction due to obesity
      and diabetes by improves insulin sensitivity and metabolic profiles to reduce the risk
      factors for cardiovascular disease and protects the vasculature through its pleiotropic
      actions on endothelial cells, endothelial progenitor cells, smooth muscle cells and
      macrophages (1). The concentrations of adiponectin of 5 to 25 mg/mL had a significant
      inhibitory effect on the expression of monocyte adhesion and adhesion molecule induced by
      TNF-α in vitro. Atherosclerosis is an inflammatory disease in which adhesion molecules on
      arterial endothelial cells are responsible for the accumulation of monocytes/macrophages and
      T lymphocytes. While obesity is low-grade inflammation in which make a contribution on
      endothelial dysfunction by increasing the oxygen-derived free radicals (ROS) due to adipocyte
      hypertrophy, leads to an endoplasmic reticulum (ER) stress and mitochondrial dysfunction (3).
      Adiponectin is accumulated in the vasculature, and it reduced on obesity due to suppression
      by TNF-α and lead to adiponectin-deficiency which stimulate the significant increases of
      Vascular cell adhesion protein 1 (VCAM-1) and Intercellular Adhesion Molecule 1 (ICAM-1) or
      known as CD54 in aortic intima (4).

      Here we investigate the level of adiponectin, ICAM-1, VCAM-1 with the incidence of MetS in
      obese adolescents.
    

Detailed description: 
      A cross sectional study with healthy obese adolescents aged 13 until 18 years old were
      conducted during October 2019 to January 2020. Obesity was determined based on body mass
      index (BMI) for age based on gender > percentile 95th of CDC growth chart 2000.

      Body weight was measured using Seca Robusta 813 digital scale in standing position, by
      stepping on the scale. While Body height was measured using Seca 206 Body Meter. Height was
      measured from the vertex of the head to the heel in standing position. Waist circumference
      and hip circumference were measured using Seca 201 measuring tape. Waist circumference was
      measured by wrapping the measuring tape around the subject's stomach, at the midpoint between
      the lowest rib and the endpoint of the iliac crest upon expiration, in line with the navel.
      Hip circumference was measured by asking the subjects to keep the feet together, then
      wrapping the measuring tape around the widest part of hips, at the point of the greatest
      gluteal protuberance. The subjects were measured using light cloth without food wear and
      other accessories such as belt, hat, or hair accessories, etc.

      Blood pressure was measure using Omron Automatic Blood Pressure Monitor HEM-8712 (Omron
      Health Care Co., Ltd, Japan) by placing the cuff on the right arm, then pull and tighten it
      according to the size if the arm. After it was installed correctly (fasten and did not move),
      the power button on the digital tension tool was pressed, and then the microprocessor started
      to drive air pressure into the cuff, and then the value of blood pressures will appear in the
      manometer tube column.

      Metabolic syndrome criteria were determined using The International Diabetic Federation
      criteria18:

        1. For adolescent aged 10-16 years old: if there was a central obesity (waist circumference
           > 90th percentile based on WHO waist circumference table. For boys at the circumference
           of > 88 cm, and ≥ 85 cm for girls) accompanied with at least 2 other signs below:

             -  Blood pressure > 90th percentile (systole ≥130/diastole ≥ 85 mmHg).

             -  Hypertriglyceridemia, if the triglyceride levels > 110 mg/dl.

             -  Low level HDL-c, if the HDL-c levels < 40 mg/dl, and < 50 mg/dl for girls.

             -  Hyperglycaemia, if fasting blood glucose (FBG) levels was > 110 mg/dl.

        2. For adolescent aged more than 16 years old: if there was a central obesity (waist
           circumference > 90th percentile based on WHO waist circumference table. For boys at the
           circumference of > 94 cm, and ≥ 80 cm for girls) accompanied with at least 2 other signs
           below:

             -  Blood pressure > 90th percentile (systole ≥130/diastole ≥ 85 mmHg).

             -  Hypertriglyceridemia, if the triglyceride levels ≥ 150 mg/dl.

             -  Low level HDL-c, if the HDL-c levels <40 mg/dl for boys, and < 50 mg/dl for girls.

             -  Hyperglycaemia, if fasting blood glucose (FBG) levels was ≥ 100 mg/dl19. Blood
                samples were withdrawn via vena cubitus as much as 5 ml by the trained analysts,
                and placed on vacutainer with EDTA. After that, the vacutainer was replaced in
                icebox to transport to the laboratory. Blood analysis include: lipid profile,
                fasting blood glucose, fasting insulin and adiponectin using the ELISA method in
                the laboratory. Intercellular Adhesion Molecule-1 (ICAM-1) was analyzed using Human
                intercellular adhesion molecule 1 Elisa kit (Bioassay Technology Laboratory). while
                sVCAM-1 was analysed using Human vascular cell adhesion molecule-1 Elisa kit
                (Bioassay Technology Laboratory).

      Statistical analysis conducted were test of normality and homogeneity test, Anova/
      Kruskal-Wallis, Independent sample T-test/Mann-Whitney U test, and Spearman correlation, and
      determined as significant if p value<0.05.
    

Intervention name(s): Nur Aisiyah Widjaja
Intervention type(s): Diagnostic Test
Intervention description(s): Blood analysis for ICAM-1, VCAM-1, blood fasting insulin, lipid profile, fasting blood glucose and adiponectin investigation
Other intervention name(s): Suhasta Nova; Leonardo Alexander Caesar
Observational model: Other
Time perspective: Cross-Sectional
Sampling method: Non-Probability Sample
Study population: 
        The subjects were obese adolescents who were studied at Junior and Senior High School in
        Surabaya and Sidoarjo, East Java, Indonesia
      
Biospecimen description: 
      Blood samples were withdrawn via vena cubitus as much as 5 ml by the trained analyst, and
      placed on vacutainer with EDTA. After that, the vacutainer was replaced in icebox to
      transport to the laboratory. Blood analysis including: lipid profile, fasting blood glucose,
      fasting insulin and adiponectin using the ELISA method in the laboratory. Intercellular
      Adhesion Molecule-1 (ICAM-1) was analyzed using Human intercellular adhesion molecule 1 Elisa
      kit (Bioassay Technology Laboratory). while sVCAM-1 was analysed using Human vascular cell
      adhesion molecule-1 Elisa kit (Bioassay Technology Laboratory).