A Serologic Study to Correlate Beta-IFN NAb Titers to Beta-IFN Induced Biomarker Response in Patients With Multiple Sclerosis
Condition: Multiple Sclerosis
MeSH terms: Multiple Sclerosis; Sclerosis
Study type: Observational

Summary: 
      Study Title: A Serologic Study to Correlate b-IFN NAb titers to b-IFN Induced Biomarker
      Response in Patients with Multiple Sclerosis

      Objectives: Subjects currently on any of the three b-IFN preparations (Avonex (Interferon
      b-1a 30 mcg IM Q 7 days), Rebif (Interferon b-1a 22 or 44mcg SC TIW), or Betaseron
      (Interferon b-1b 250mcg SC QOD)) will be enrolled to one of 3 groups:

      Group 1: Approximately 200 subjects will be enrolled who are NAb+ (titer ³ 20NU/ml) Group 2:
      Approximately 50 subjects will be enrolled who are BAb- (titer <8U) and NAb- (titer <20
      NU/ml) Group 3: Approximately 50 subjects will be enrolled who are BAb+ (titer ³8U) and NAb-
      (titer <20 NU/ml)

      The primary objective of this study is to compare baseline b-IFN induced MxA mRNA response in
      neutralizing antibody positive (NAb+, titers ³ 20NU/ml, Group 1) vs. antibody negative (NAb-,
      titers < 20NU/ml, BAb-, titers <8U, Group 2) patients.

      Secondary objectives are:

        1. To compare b-IFN induced MxA mRNA response in neutralizing antibody positive (NAb+,
           titers ³ 20NU/ml, Group 1) vs. antibody negative (NAb-, titers < 20NU/ml, BAb-, titers
           <8U, Group 2) patients at the month 6 visit.

        2. To compare b-IFN induced biomarker response (viperin, IFIT1) in neutralizing antibody
           positive (NAb+, titers ³ 20NU/ml, Group 1) vs. antibody negative (NAb-, titers <
           20NU/ml, BAb-, titers <8U, Group 2) patients (data compared at baseline and month 6
           visits)

        3. To compare b-IFN induced biomarker response (MxA, viperin, IFIT1) in neutralizing
           antibody positive (NAb+, titers ³ 20NU/ml, Group 1) vs. BAb+ (titers ³8U)/NAb- (titers
           <20 NU/ml, Group 3) patients at baseline and month 6 visits.

        4. To correlate NAb titer levels with b-IFN induced biomarker response (data taken from
           baseline and month 6 visits from Group 1).

        5. To compare b-IFN induced biomarker response (MxA, viperin, IFIT1) in BAb-/NAb- patients
           (Group 2) vs. BAb +/NAb- patients (Group 3) in order to determine if BAbs affect b-IFN
           induced biomarker response (data compared at baseline and month 6 visits).

      Tertiary objectives are:

      1. To explore patient characteristics that may predict NAb positivity.

      Design: This is a multi-center, open-label study of approximately 300 (200 NAb+ and 100 NAb-
      (50 BAb+ and 50 BAb-)) MS patients that will compare b-IFN induced biomarker response
      following b-IFN injection in neutralizing antibody positive vs. antibody negative patients.

      Study Population: Approximately 300 subjects (200 in Group 1, 50 in Group 2, and 50 in Group
      3) will be recruited for the study.

      Inclusion Criteria: To be eligible for entry into this study, candidates must meet all of the
      following eligibility criteria at the time of randomization:

        1. Patients (male or female) diagnosed with relapsing forms of MS.

        2. Currently being treated with the same b-IFN (in accordance with FDA approved dosing and
           schedules) for 12 to 48 months inclusive.

        3. All levels of disability

        4. Age 18-65 years inclusive

        5. Subjects must be willing to be followed for the 6-month study period.

      Exclusion Criteria: Candidates will be excluded from study entry if any of the following
      exclusion criteria exist at the time of study initiation.

        1. Patients with prior b-IFN NAb test (whether positive or negative).

        2. Patients who are currently being treated or have been treated within 6 months prior to
           screening with combination therapy (b-IFN plus any other
           immunosuppressant/immunomodulatory) other than IV steroids (either pulse or for a
           relapse).

        3. Unwillingness or inability to comply with the requirements of this protocol including
           the presence of any condition (physical, mental, or social) that is likely to affect the
           subject's ability to comply with the study protocol.

        4. Any other reasons that, in the opinion of the Investigator, the subject is determined to
           be unsuitable for enrollment into this study.

      Treatment Groups: This is a multi-center, open-label study of approximately 300 (200 NAb+
      patients and 100 NAb- patients (50 BAb+ and 50 BAb-)) relapsing MS patients that will compare
      b-IFN induced biomarker response following b-IFN injection in neutralizing antibody positive
      vs. antibody negative patients.

      Subjects currently on any of the three b-IFN preparations (Avonex (Interferon b-1a 30 mcg IM
      Q 7 days), Rebif (Interferon b-1a 22 or 44mcg SC TIW), or Betaseron (Interferon b-1b 250mcg
      SC QOD)) will be enrolled to one of 3 groups:

      Group 1: Approximately 200 subjects will be enrolled who are NAb+ (titer ³ 20NU/ml)

      Group 2: Approximately 50 subjects will be enrolled who are BAb- (titer <8U) and NAb- (titer
      <20 NU/ml)

      Group 3: Approximately 50 subjects will be enrolled who are BAb+ (titer ³8U) and NAb- (titer
      <20 NU/ml)
    


Observational model: Case-Control
Time perspective: Prospective
Sampling method: Non-Probability Sample
Study population: 
        MS patients on interferon beta
      
Biospecimen description: 
      whole blood and serum
    
Keywords: MS