passages.id: split_8_test_525_passage
passages.type: progene_text
passages.text: However , an i31 peptide insertion at Tyr39 had no effect on protein function as defined by the capacity of the mutant protein to (i) interact with native VirE2 , (ii) interact with VirE1 , (iii) accumulate at abundant levels in A. tumefaciens , and (iv) restore wild - type virulence to a virE2 null mutant .