passages.id: split_8_test_259_passage
passages.type: progene_text
passages.text: Rat hepatocytes were then transfected with constructs containing the first 493 bp of the PCK1 5' - flanking region in front of the luciferase ( LUC ) reporter gene , which were block - mutated at the CRE1 ( cAMP - response element-1 ; -93/-86) , putative CRE2 ( - 146 / - 139 ) , promoter element ( P ) 1 (-118/-104) , P2 (-193 / - 181 ) or P4 ( - 291 / - 273 ) sites .