passages.id: split_8_test_2142_passage
passages.type: progene_text
passages.text: Cytokine - free human hepatoma Hep3B cells or cells treated with interleukin-6 ( IL-6 ) or orthovanadate ( a general activator of STATs ) were fractionated , and plasma membrane raft fractions were obtained by equilibrium sedimentation or flotation through discontinuous sucrose gradients using either non - detergent or detergent - based ( saponin or Triton X - 100 ) methods .