passages.id: split_8_test_655_passage
passages.type: progene_text
passages.text: The results showed that Sp1 protein enhanced the Bmax of NF-Y - DNA binding by more than 5 - fold ( on - rate ) ; in addition , the Sp1 - enhanced NF-Y - DNA complex was further stabilized by coincubation with ER and the rate of dissociation ( t1 / 2 ) was decreased by approximately 50 % .