passages.id: split_8_test_310_passage
passages.type: progene_text
passages.text: Replacement by Ala of one of the four Asp residues , invariant in all representatives of protein phosphatase 2C , completely abolished the SpoIIE phosphatase activity in vitro , whilst replacement of the Asp residues by another acidic amino acid , Glu , had varying effects on the activities of the resulting mutated proteins .