passages.id: split_8_test_799_passage
passages.type: progene_text
passages.text: By using site - directed mutagenesis and ectopic expression of the alphaL / beta2 integrin in Chinese hamster ovary cells , we found that Y735 in the membrane - proximal YRRF sequence is selectively required for recycling of spontaneously internalized receptors to the cell surface and to growth factor - induced membrane ruffles .