passages.id: split_8_test_1160_passage
passages.type: progene_text
passages.text: beta-III - Tubulin was used as a specific Purkinje cell marker protein , immunohistochemical localization showed strong beta - III - tubulin immunoreactivity ( IR ) in Purkinje cells in 6 week old heterozygous TM , whereas CaB and PV IR were markedly reduced in the same neurons ( double immunofluorescence staining ) .