passages.id: split_6_test_3339_passage
passages.type: progene_text
passages.text: In addition , we used CDR3 length spectratyping , CDR3 - specific reverse transcriptase - PCR and direct TcR sequencing to show that the TcR beta chain structural patterns associated with p530 - specific T cells consistently appeared in the islets of young NOD mice with insulitis , but not in the inflamed islets of streptozotocin - treated C57BL / 6 mice , or in inflamed NOD salivary glands .