passages.id: split_6_test_95_passage
passages.type: progene_text
passages.text: UV - irradiated plasmids were transfected into a human RKO colon carcinoma cell line that contains wild - type ( wt ) p53 as well as its derivatives , either mutant p53 - 143ala ( RKO-143ala ) or human papillomavirus E6 ( RKO - E6 , a wt p53 protein that is rapidly degraded and non - functional ) .