passages.id: split_6_test_435_passage
passages.type: progene_text
passages.text: After centrifugation of 300 microL fractions of whole semen , pellets were resuspended either in autologous SP or in a chemically defined medium ( BM ) supplemented or not with 3 % bovine serum albumin ( BSA ) ; after 15 min at 37 degrees C , the samples were diluted ( V / V ) with cryoprotective medium ( 30 mM NaCl ; 22 mM sodium citrate , 19.4 mM fructose ; 80 mM glutamine ; 14 % , V / V , glycerol ) and maintained for 15 min at room temperature before freezing .