passages.id: split_6_test_1547_passage
passages.type: progene_text
passages.text: Sixteen patients with metastatic stage IV melanoma were treated with use of intravenous infusions of dendritic cells ( DC ) derived by incubation of plastic - adherent peripheral blood mononuclear cells ( PBMC ) with IL-4 and GM-CSF for 8 days in serumless AIM - V medium , followed by overnight pulsing with peptides .