passages.id: split_6_test_1475_passage
passages.type: progene_text
passages.text: For endothelial cells , we examined the expression of intercellular adhesion molecule-1 ( ICAM-1 ) , the AT1 receptor , and glucose transporter-1 ( GLUT-1 , a marker of the blood - brain barrier ) using reverse transcription - polymerase chain reaction ( RT - PCR ) .