passages.id: split_6_test_1826_passage
passages.type: progene_text
passages.text: Using reverse transcription - polymerase chain reaction ( RT - PCR ) with degenerate oligonucleotides corresponding to two highly conserved motifs within the protein kinase family of catalytic domains , we isolated a PCR fragment encoding a novel member of the testis - specific serine / threonine kinases ( STK ) from mouse male mixed germ cell mRNA .