passages.id: split_6_test_436_passage
passages.type: progene_text
passages.text: Assessment of viability and motility was performed using fresh semen ( T0 ) , after centrifugation and resuspension prior to adding the cryoprotectant ( T15 ) , after adding the cryoprotectant ( T30 ) and after freezing and thawing ( Tpost ) .