passages.id: split_5_test_3008_passage
passages.type: progene_text
passages.text: The 3-ketoacyl-acyl carrier protein synthase III gene ( fabH ) from E. coli was modified by saturation point mutagenesis at the codon encoding amino acid 87 of the FabH protein sequence , and the resulting plasmids were cotransformed with either the pAPAC plasmid , which harbors the Aeromonas caviae PHA synthase gene ( phaC ) , or the pPPAC plasmid , which harbors the Pseudomonas sp. strain 61-3 PHA synthase gene ( phaC1 ) , and the abilities of these strains to accumulate PHA from glucose were assessed .