passages.id: split_5_test_3641_passage
passages.type: progene_text
passages.text: Using the yeast two - hybrid system , Alam et al. ( Alam , M. R. , Caldwell , B. D. , Johnson , R. C. , Darlington , D. N. , Mains , R. E. , and Eipper , B. A. ( 1996 ) J. Biol. Chem. 271 , 28636 ) identified three proteins that interact with a fragment of the PAM cytosolic domain containing these targeting signals .