passages.id: split_5_test_1483_passage
passages.type: progene_text
passages.text: Kinetic properties and the FMN content of these mutants were not affected by these substitutions , with the exception of a 3 - fold increase in Y456S K(m) ( cyt )(c) and a 70 % decrease in R454E FMN content , suggesting that the FMN - and FAD - binding domains are largely , but not completely , independent .