passages.id: split_5_test_1317_passage
passages.type: progene_text
passages.text: Various concentrations of 20 kDa and 22 kDa GH ( 0 , 2 , 5 , 12.5 , 25 , 50 and 150 ng / ml ) were added to human hepatoma ( HuH7 ) cells cultured in serum - free hormonally defined medium for 0 , 1 and 2 h .