passages.id: split_5_test_1308_passage
passages.type: progene_text
passages.text: Using reverse transcription - polymerase chain reaction ( RT - PCR ) , Western immunoblotting , and ELISA to evaluate NGF expression and release , we found that subjects with allergic rhinitis have significantly decreased NGF mRNA in superficial nasal scrapings and significantly higher baseline concentrations of NGF protein in nasal lavage fluids , compared with control subjects .