passages.id: split_4_validation_1303_passage
passages.type: progene_text
passages.text: A total of 49 malignant pleural effusions and 68 corresponding solid tumors were studied for protein and mRNA expression of vascular endothelial growth factor ( VEGF ) and its receptor KDR , interleukin-8 ( IL-8 ) , basic fibroblast growth factor ( bFGF ) and the alphaV integrin subunit using immunohistochemistry , mRNA in situ hybridization ( ISH ) and reverse transcription polymerase chain reaction ( RT - PCR ) .