passages.id: split_4_validation_1183_passage
passages.type: progene_text
passages.text: Northern blotting was used to demonstrate that 10 microg per ml insulin and 0.1 microg per ml epidermal growth factor both increased steady - state levels of 3-hydroxy-3-methylglutaryl coenzyme A synthase mRNA by 2.5 and 6 - fold , respectively .