passages.id: split_4_validation_1023_passage
passages.type: progene_text
passages.text: Blood samples were taken via subclavian central vein at the time before anesthesia ( T1 ) , 10 minutes of occlusion of aorta ( T2 ) , 10 minutes ( T3 ) and 30 minutes ( T4 ) after the release , and the end of operation ( about 180 minutes after release , T5 ) respectively ; EKG was observed , and the levels of asparate aminotransferase ( AST ) , lactate dehydrogenase ( LDH ) , creatine kinase ( CK ) , MB isoenzyme of CK ( CK-MB ) , malondialdehyde ( MDA ) and the activity of superoxide dismutase ( SOD ) , nitric oxide ( NO ) , nitric oxide synthetase ( NOS ) were determined .