passages.id: split_4_validation_1331_passage
passages.type: progene_text
passages.text: The specificity of the interaction was validated by showing that the PLD1 D4 fragment interacts with glutathione S-transferase - RhoA in vitro in a GTP - dependent manner and that it associates with RhoAVal-14 in COS-7 cells , whereas the N - terminal two - thirds of PLD1 does not .