77407811-6807-4e23-a9c4-93702aa2442b
title
The  glutathione S-transferase  polymorphisms in a control population and in Alzheimer's disease patients.
c22fe894-2896-4a89-8717-df465dad956c
abstract
In this study, we investigated the role of  glutathione S-transferase P1  ( GSTP1 ) polymorphisms in the pathogenesis of Alzheimer's disease (AD). We genotyped the  GSTP1  polymorphisms in exon 5 ( A313G ) and exon 6 ( C341T ) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 56 Croatian patients with AD and 231 controls. Distributions and frequencies of  GSTP1  genetic variants were not statistically different between AD patients and healthy controls. Higher frequencies of the mutant genotypes were observed in AD patients (13% for both  A313G  and  C341T ) when compared with control subjects (7% for  A313G  and 8% for  C341T ), but association of  GSTP1  GG (OR 2.057, 95% CI 0.796-5.315, p=0.094) and TT (OR 1.691, 95% CI 0.669-4.270, p=0.514) genotypes with an increased risk of AD was not confirmed by statistical analysis. The frequencies of  GSTP1  alleles (A, B, C, D) did not significantly differ between AD patients and controls and they were indicated as follows: 52.7%, 15.2%, 12.5% and 19.6% for AD cases and 58.4%, 14.1%, 14.1% and 13.4% for controls. The estimation of the  GSTP1  haplotype distribution showed that  GSTP1*A/GSTP1*B  and  GSTP1*A/GSTP1*C  haplotypes were less frequent, while  GSTP1*B/GSTP1*B  and  GSTP1*C/GSTP1*D  haplotypes were more frequent in AD patients than in controls. In conclusion, the involvement of  GSTP1  alleles in individual susceptibility to AD was not confirmed as statistically significant in the tested Croatian Caucasian population. A possible role of  GSTP1  in the complex etiopathogenesis of AD is further discussed, based on observed differences in haplotype distribution and higher frequencies of mutant genotypes in AD patients.