7d4b847d-a907-44cf-8d37-636dcbc69fc0
title
Association of single nucleotide polymorphisms within cytokine genes with juvenile idiopathic arthritis in the Czech population.
e214b9ae-ce9a-4d10-9147-1c6aa177fc75
abstract
OBJECTIVE: To examine the possible association of juvenile idiopathic arthritis (JIA) with polymorphisms within cytokine genes in the Czech population. METHODS: In a case-control study, genotypes of 130 patients with JIA (63 male, 67 female; age at onset 7.6 +/- 4.4 yrs; 43 oligoarticular, 72 polyarticular, 15 systemic form) were compared to 102 healthy unrelated blood donors. Using the polymerase chain reaction technique with sequence-specific primers from the 13th IHWG workshop, we analyzed 19 single nucleotide polymorphisms within 12 different cytokine genes [ interleukin (IL)-1a ,  IL-1beta ,  IL-2 ,  IL-4 ,  IL-6 ,  IL-10 ,  IL-12 ,  transforming growth factor (TGF)-beta ,  interferon (IFN)-g ], and related molecules ( IL-1R ,  IL-1RA ,  IL-4Ra ). Genotype frequencies were compared using chi-square analysis, and the significance level was corrected for the number of independent tests. RESULTS: Significant positive association was found for the G allele of the  IL-4   -1098 T/G  polymorphism, which was carried by 10% of cases and 25% of controls [odds ratio (OR) 0.32, 95% confidence interval (CI) 0.16-0.67, corrected p = 0.038). Also, a nonsignificant increase in the frequency of the  IL-1beta   +3962 C  allele was detected in cases (96%) versus controls (84%) (OR 4.65, 95% CI 1.64-13.2, corrected p = 0.091). We did not replicate previously found associations with the  IL-1a ,  IL-6 ,  IL-10 , and  IL-1R IL-1RA  polymorphisms. CONCLUSION: Our study showed association with JIA for the  IL-4   -1098 T/G  polymorphism. It also underlines the genetic contribution of IL-1 polymorphisms to the pathogenesis of JIA, as another polymorphism within the  IL-1beta  may influence the risk of the disease.