sentences.id: IEPA.d115.s277
sentences.origID: 40
sentences.text: Overexpression of G11alpha and isoforms of phospholipase C in islet  beta-cells reveals a lack of correlation between inositol phosphate  accumulation and insulin secretion.
sentences.id: IEPA.d115.s277.e0
sentences.origID: 95
sentences.text: phospholipase C
sentences.id: IEPA.d115.s277.e1
sentences.origID: 96
sentences.text: insulin
sentences.id: IEPA.d115.s278
sentences.origID: 41
sentences.text: It has been suggested that insulin secretion from pancreatic islets may be  mediated in part by activation of phospholipases C (PLCs) and  phosphoinositide hydrolysis.
sentences.id: IEPA.d115.s278.e0
sentences.origID: 97
sentences.text: insulin
sentences.id: IEPA.d115.s278.e1
sentences.origID: 98
sentences.text: phospholipases C
sentences.id: IEPA.d115.s278.i0
sentences.e1: IEPA.d115.s278.e0
sentences.e2: IEPA.d115.s278.e1
sentences.type: PPI
sentences.id: IEPA.d115.s279
sentences.origID: 42
sentences.text: The purpose of this study was to determine  whether the relatively modest fuel-stimulated insulin secretion responses  of rodent beta-cell lines might be explained by inadequate expression or  activation of PLC isoforms.
sentences.id: IEPA.d115.s279.e0
sentences.origID: 99
sentences.text: insulin
sentences.id: IEPA.d115.s279.e1
sentences.origID: 100
sentences.text: PLC
sentences.id: IEPA.d115.s279.i0
sentences.e1: IEPA.d115.s279.e0
sentences.e2: IEPA.d115.s279.e1
sentences.type: PPI
sentences.id: IEPA.d115.s280
sentences.origID: 43
sentences.text: Adenovirus-mediated overexpression of PLC-delta1, -beta1, or  -beta3 in INS-1 or betaG 40/110 cells results in little or no enhancement  in inositol phosphate (IP) accumulation and no improvement in insulin  secretion when the cells are stimulated with glucose or carbachol, despite  the fact that the overexpressed proteins are fully active in cell  extracts.
sentences.id: IEPA.d115.s280.e0
sentences.origID: 101
sentences.text: PLC
sentences.id: IEPA.d115.s280.e1
sentences.origID: 102
sentences.text: insulin
sentences.id: IEPA.d115.s281
sentences.origID: 44
sentences.text: Overexpression of PLC-beta1 or -beta3 in normal rat islets  elicits a larger increase in IP accumulation but, again, has no effect on  insulin secretion.
sentences.id: IEPA.d115.s281.e0
sentences.origID: 103
sentences.text: PLC
sentences.id: IEPA.d115.s281.e1
sentences.origID: 104
sentences.text: insulin
sentences.id: IEPA.d115.s282
sentences.origID: 45
sentences.text: Because the effect of carbachol on insulin secretion is  thought to be mediated through muscarinic receptors that link to the Gq/11  class of heterotrimeric G proteins, we also overexpressed G11alpha in  INS-1 cells, either alone or in concert with overexpression of PLC-beta1  or -beta3.
sentences.id: IEPA.d115.s282.e0
sentences.origID: 105
sentences.text: insulin
sentences.id: IEPA.d115.s282.e1
sentences.origID: 106
sentences.text: PLC
sentences.id: IEPA.d115.s283
sentences.origID: 46
sentences.text: Overexpression of G11alpha enhances IP accumulation, an effect  slightly potentiated by co-overexpression of PLC-beta1 or -beta3, but  these maneuvers do not affect glucose or carbachol-stimulated insulin  secretion.
sentences.id: IEPA.d115.s283.e0
sentences.origID: 107
sentences.text: PLC
sentences.id: IEPA.d115.s283.e1
sentences.origID: 108
sentences.text: insulin