sentences.id: IEPA.d58.s150
sentences.origID: 253
sentences.text: We have recently shown that AVP causes a protein kinase C (PKC)-dependent increase in ACTH release and biosynthesis in ovine anterior pituitary cells
sentences.id: IEPA.d58.s150.e0
sentences.origID: 547
sentences.text: AVP
sentences.id: IEPA.d58.s150.e1
sentences.origID: 548
sentences.text: protein kinase C
sentences.id: IEPA.d58.s150.i0
sentences.e1: IEPA.d58.s150.e0
sentences.e2: IEPA.d58.s150.e1
sentences.type: PPI
sentences.id: IEPA.d58.s151
sentences.origID: 254
sentences.text: In these cells, AVP also causes the translocation of PKC from the cytosol to the cell membrane which is maximal at 5 min, but the intracellular events distal to protein kinase C activation that underlie ACTH secretion have not been well characterized to date
sentences.id: IEPA.d58.s151.e0
sentences.origID: 549
sentences.text: AVP
sentences.id: IEPA.d58.s151.e1
sentences.origID: 550
sentences.text: PKC
sentences.id: IEPA.d58.s151.e2
sentences.origID: 551
sentences.text: protein kinase C
sentences.id: IEPA.d58.s151.i0
sentences.e1: IEPA.d58.s151.e0
sentences.e2: IEPA.d58.s151.e1
sentences.type: PPI
sentences.id: IEPA.d58.s152
sentences.origID: 255
sentences.text: Since the MARCKS protein has been implicated in neurosecretion and is phosphorylated by PKC in synaptosomes, studies were carried out to determine whether AVP might cause MARCKS phosphorylation in the ovine anterior pituitary, and to determine whether this phenomenon might be temporally correlated with PKC translocation and the release of ACTH
sentences.id: IEPA.d58.s152.e0
sentences.origID: 552
sentences.text: PKC
sentences.id: IEPA.d58.s152.e1
sentences.origID: 553
sentences.text: AVP
sentences.id: IEPA.d58.s152.e2
sentences.origID: 554
sentences.text: PKC
sentences.id: IEPA.d58.s153
sentences.origID: 256
sentences.text: We conclude that: (1) AVP causes a rapid, and reversible, phosphorylation of the MARCKS protein in the ovine anterior pituitary; (2) since the AVP-induced increase in MARCKS phosphorylation occurs much earlier in these cells than does PKC trans-location, MARCKS phosphorylation may provide a more sensitive index of the onset of PKC activation than the translocation assay; (3) the close temporal association between MARCKS phosphorylation and the rapid early release of ACTH suggests that MARCKS phosphorylation may be involved in the initial intracellular events that underly exocytosis of the hormone.
sentences.id: IEPA.d58.s153.e0
sentences.origID: 555
sentences.text: AVP
sentences.id: IEPA.d58.s153.e1
sentences.origID: 556
sentences.text: AVP
sentences.id: IEPA.d58.s153.e2
sentences.origID: 557
sentences.text: PKC
sentences.id: IEPA.d58.s153.e3
sentences.origID: 558
sentences.text: PKC