text:  A549 rho vitro 2 cultured cells A549 rho zero cells cultured in RPMI 1640 medium supplemented with 20 mM HEPES, 2 mM L-glutamine, 10% heat inactivated fetal bovine serum (Hyclone) and antibiotics (200 U/mL penicillin and 200 μg/mL streptomycin).