text:  Subject6_EIB_negative_post_exercise Induced sputum from EIB negative person Induced sputum was conducted with 3% hypertonic saline via an ultrasonic nebulizer (DeVilbiss, Somerset, PA, USA). At 2-min intervals, subjects were asked to clear saliva from their mouth and then expectorate sputum. Sputum was collected over 20 min and was pooled into a single sample container. The induced sputum was placed on ice immediately and processed within 15 min of collection.