text:  Zinc A non-cycling plateau phase A549 lung cancer cell culture A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.