tokens: In all three siblings , we demonstrated the presence of anergic CD3 + CD4 - CD8 - ( double negative , [ DN ] ) T cells ; moreover , a chronic lymphocyte activation was found , as demonstrated by the presence of high levels of HLA - DR expression on peripheral CD3 + cells and by the presence of high levels of serum activation markers such as soluble interleukin - 2 receptor ( slL - 2R ) and soluble CD30 ( sCD30 ) . .