tokens: A single clone , which was conserved and had near - perfect homology to eight human / rodent expressed sequence tags , was used as template for 5 ' and 3 ' rapid amplification of cDNA ends and SPICE ( system for polymerase chain reaction amplification of cDNA ends ) reactions to obtain the 3 . 6 - kb cDNA , LGL2 ( Genbank , AF 110195 ) encoding a deduced polypeptide ( lgl2 ) of 963 amino acids .