passages.id: 1.0alpha7.train.284.sent
passages.type: sentence
passages.text: For in vitro kinase assays, 15 μl of GST or GST-Cx43CT bound to glutathione-Sepharose 4B beads (washed once in kinase buffer) were incubated with purified kinase-active pp60 c-src, kinase buffer (20 mM HEPES (pH 7.4), 12 mM MnCl2, 1 mM dithothreitol, 100 μM Na2VO4) and 10 μCi of ATP (DuPont NEN, NEG-002Z, 6000 Ci/mmol) in a total volume of 40 μl for 15 min at room temperature.
coreferences.id: 1.0alpha7.train.284.coref0
coreferences.entity_ids: 1.0alpha7.train.284.ent0_0 1.0alpha7.train.284.ent1_1
coreferences.id: 1.0alpha7.train.284.coref1
coreferences.entity_ids: 1.0alpha7.train.284.ent2_0
coreferences.id: 1.0alpha7.train.284.coref2
coreferences.entity_ids: 1.0alpha7.train.284.ent3_0
coreferences.id: 1.0alpha7.train.284.coref3
coreferences.entity_ids: 1.0alpha7.train.284.ent4_0
coreferences.id: 1.0alpha7.train.284.coref4
coreferences.entity_ids: 1.0alpha7.train.284.ent5_0
coreferences.id: 1.0alpha7.train.284.coref5
coreferences.entity_ids: 1.0alpha7.train.284.ent6_0