passages.id: 1.0alpha7.train.1084.sent
passages.type: sentence
passages.text: Using purified glutathione S-transferase (GST)-fused wild-type (WT) Aurora B, GST-fused kinase-defective (KD) mutant of Aurora B, or GST alone against purified maltose binding protein (MBP)-fused MgcRacGAP, GST pull-down assays were performed in buffer A (25 mM Tris [pH 7.5], 2 mM MgCl2, 200 μM ATP, 0.1 μM calyculin A, 1% Nonidet P-40).
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