passages.id: 1.0alpha7.train.1116.sent
passages.type: sentence
passages.text: To evaluate cadherin- or TCF-binding capacity of β-catenin in SW480 cells, a cell lysate (1% nonidet 40–containing buffer) or 100,000-g detergent-free supernatant (Reinacher-Schick and Gumbiner 2001) was subjected to consecutive affinity precipitations (30′ incubations × 3) with a glutathione S-transferase (GST) fusion protein containing the cytoplasmic domain of C-cadherin (Yap et al. 1998) or the β-catenin binding region of Xenopus TCF-3.
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