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passages.type: sentence
passages.text: This conclusion is based on studies carried out with consensus Sp1 oligonucleotide showing that (i) Sp1 binding activity was present in nuclear extracts from all three cell types studied; (ii) Sp1 binding was 2–3-fold greater in nuclear extracts from chondrocytes dedifferentiated into fibroblast-like cells by passage in monolayer culture on plastic substrate than in freshly isolated chondrocytes or in chondrocytes allowed to maintain their phenotype by culture on polyHEMA-coated dishes; (iii) Sp1 binding was specific, since it was competed by unlabeled Sp1 and not by AP1; (iv) Sp1 binding was enhanced by KCl and inhibited by the addition of EDTA; (v) A polyclonal antibody against Sp1 decreased the binding of Sp1 by 85% in chondrocytes dedifferentiated into fibroblast-like cells but caused only a very slight inhibition in freshly isolated chondrocytes or in chondrocytes cultured in suspension on polyHEMA.
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