passages.id: 1.0alpha7.train.1356.sent
passages.type: sentence
passages.text:  To ask whether phosphorylation of  pRB under the control of a heterologous KRXL motif was functionally significant, asynchronously growing SAOS2 cells were transfected with  plasmids encoding HA-tagged pRB(1-864) fused to 12 amino acids (GRPPVKRRLDLE) spanning the wild-type  cyclin-cdk2 binding sequence of  E2F1 [pRB(1-864) E21yWT] or HA-tagged  pRB(1-928) in the presence or absence of  plasmids encoding  cyclin A and  cdk2.  
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