Both synthetic and naturally derived crosslinkers have been found to exhibit significant effects in biomodification of dentine via their multiple interactions with the dentine matrix .
A stable matrix network or a durable hybrid layer in dentine bonding could be achieved , where the dentine collagen fibrils show improved biochemical and biomechanical properties and enzymatic biodegradation is reduced .
This aim of this review is to explore the current research related to  crosslinking agents used on dentine .
Effect of Carbodiimide on Bonding Durability of Adhesive-cemented Fiber Posts in Root Canals.
This study was undertaken to investigate whether using a protein cross-linker , 1-ethyl-3-(3-dimethylaminopropyl ) carbodiimide ( EDC ) , improves bonding stability of fiber posts to root dentin using three resin cements . Sixty human maxillary central incisor roots were r and omly divided into six groups after endodontic treatment , according to the cements used with and without EDC pretreatment . In the etch- and -rinse group , 0.3 M EDC aqueous solution was applied on acid-etched root dentin prior to Excite DSC/Variolink II for post cementation . In the self-etch and self-adhesive groups , EDC was used on EDTA-conditioned root space prior to application of ED Primer II/Panavia F2.0 and Clearfil SA , respectively . After microslicing the root dentin , a push-out bond strength ( BS ) test was performed immediately or after one-year of water storage for each group . Data were analyzed using three-way analysis of variance and Tukey tests ( α=0.05 ) . A significant effect of cement type , time , EDC , and Time × Cement and Time × EDC interactions were observed ( p≤0.001 ) . EDC pretreatment did not affect immediate bonding of the three cements ( p>0.05 ) . Aging significantly reduced the BS in all the groups ( p≤0.001 ) , but EDC groups exhibited a higher BS compared with the respective control groups ( p<0.001 ) . Despite the significant effect of aging on decreasing the BS of fiber post to radicular dentin , EDC could diminish this effect for the three tested cements
Both synthetic and naturally derived crosslinkers have been found to exhibit significant effects in biomodification of dentine via their multiple interactions with the dentine matrix .
A stable matrix network or a durable hybrid layer in dentine bonding could be achieved , where the dentine collagen fibrils show improved biochemical and biomechanical properties and enzymatic biodegradation is reduced .
This aim of this review is to explore the current research related to  crosslinking agents used on dentine .