Conclusions : The application of a 2 % chlorhexidine solution after acid etching increased the microtensile bond strength significantly for follow-up times of 6 months or more .
The adhesive type had no influence
Background and Objectives : The aim of this systematic review was to examine the effect of 2 % chlorhexidine following acid etching on the microtensile bond strength of resin restorations for different follow-up times .
Collagen Degradation by Host-derived Enzymes during Aging
Incompletely infiltrated collagen fibrils in acid-etched dentin are susceptible to degradation . We hypothesize that degradation can occur in the absence of bacteria . Partially demineralized collagen matrices ( DCMs ) prepared from human dentin were stored in artificial saliva . Control specimens were stored in artificial saliva containing proteolytic enzyme inhibitors , or pure mineral oil . We retrieved them at 24 hrs , 90 and 250 days to examine the extent of degradation of DCM . In the 24-hour experimental and 90- and 250-day control specimens , we observed 5- to 6-μm-thick layers of DCM containing b and ed collagen fibrils . DCMs were almost completely destroyed in the 250-day experimental specimens , but not when incubated with enzyme inhibitors or mineral oil . Functional enzyme analysis of dentin powder revealed low levels of collagenolytic activity that was inhibited by protease inhibitors or 0.2 % chlorhexidine . We hypothesize that collagen degradation occurred over time , via host-derived matrix metalloproteinases that are released slowly over time
Conclusions : The application of a 2 % chlorhexidine solution after acid etching increased the microtensile bond strength significantly for follow-up times of 6 months or more .
The adhesive type had no influence
Background and Objectives : The aim of this systematic review was to examine the effect of 2 % chlorhexidine following acid etching on the microtensile bond strength of resin restorations for different follow-up times .