Both synthetic and naturally derived crosslinkers have been found to exhibit significant effects in biomodification of dentine via their multiple interactions with the dentine matrix .
A stable matrix network or a durable hybrid layer in dentine bonding could be achieved , where the dentine collagen fibrils show improved biochemical and biomechanical properties and enzymatic biodegradation is reduced .
This aim of this review is to explore the current research related to  crosslinking agents used on dentine .
Occlusal loading and cross-linking effects on dentin collagen degradation in physiological conditions.
OBJECTIVE This study evaluated the ability of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride ( EDC ) to improve the stability of demineralized dentin collagen matrices when subjected to mechanical cycling by means of Chewing Simulation ( CS ) . METHODS Demineralized dentin disks were r and omly assigned to four groups ( N=4 ) : ( 1 ) immersion in artificial saliva at 37 ° C for 30 days ; ( 2 ) pre-treatment with 0.5 M EDC for 60 s , then stored as in Group 1 ; ( 3 ) CS challenge ( 50 N occlusal load , 30 s occlusal time plus 30 s with no load , for 30 days ) ; ( 4 ) pre-treatment with 0.5 M EDC as in Group 2 and CS challenge as in Group 3 . Collagen degradation was evaluated by sampling storage media for ICTP and CTX telopeptides . RESULTS EDC treated specimens showed no significant telopeptides release , irrespective of the aging method . Cyclic stressing of EDC-untreated specimens caused significantly higher ICTP release at day 1 , compared to static storage , while by days 3 and 4 , the ICTP release in the cyclic group fell significantly below the static group , and then remained undetectable from 5 to 30 days . CTX release in the cyclic groups , on EDC-untreated control specimens was always lower than in the static group in days 1 - 4 , and then fell to undetectable for 30 days . SIGNIFICANCE This study showed that chewing stresses applied to control untreated demineralized dentin increased degradation of collagen in terms of CTX release , while collagen crosslinking agents may prevent dentin collagen degradation , irrespective of simulated occlusal function
Both synthetic and naturally derived crosslinkers have been found to exhibit significant effects in biomodification of dentine via their multiple interactions with the dentine matrix .
A stable matrix network or a durable hybrid layer in dentine bonding could be achieved , where the dentine collagen fibrils show improved biochemical and biomechanical properties and enzymatic biodegradation is reduced .
This aim of this review is to explore the current research related to  crosslinking agents used on dentine .