Conclusions In light of the currently available scientific evidence , dentin surface pretreatment with deproteinizing agents does not enhance the bonding of SE adhesives to dentin .
The HOCl deproteinizing agent exhibited minimal adverse effects on bonding to dentin in comparison with NaOCl solutions
Objectives The aim of this systematic review was to critically analyze previously published studies of the effects of dentin surface pretreatment with deproteinizing agents on the bonding of  self-etch ( SE ) adhesives to dentin .
Additionally , a meta- analysis was conducted to quantify the effects of the above-mentioned surface pretreatment methods on the bonding of SE adhesives to dentin .
Influence of collagen removal on shear bond strength of one-bottle adhesive systems in dentin.
PURPOSE The aim of this study was to evaluate the influence of collagen removal on shear bond strength ( SBS ) of three hydrophilic one-bottle adhesive systems in dentin . MATERIAL S AND METHODS Test specimens were taken from one-hundred twenty bovine incisors . The specimens were debrided , polished with 320- to 600-grit Al2O3 paper , and r and omly divided into 6 groups ( n = 20 ) : G1 : acid/10 % NaOCl + Bond 1 ( B1H ) ; G2 : acid + Bond 1 applied according to manufacturer 's instructions ( B1 ) ; G3 : acid/10 % NaOCl + Optibond Solo ( OSH ) ; G4 : acid + Optibond Solo used according to manufacturer 's instructions ( OS ) ; G5 : acid/10 % NaOCl + Single Bond ( SBH ) ; G6 : acid + Single Bond used according to manufacturer 's instructions . In all groups , dentin was etched for 15 s , rinsed , and dried . In groups 1 , 3 , and 5 , the 10 % NaOCl was applied with a dwell time of 60 s , rinsed , and dried . In all groups , the adhesive systems were applied and light cured , and then a restorative composite resin ( Z-100 ) was inserted in a teflon matrix and cured . The specimens were stored in humid conditions for 7 days at 37 degrees C. The SBS tests were performed in an EMIC universal testing machine with a crosshead speed of 0.5 mm/min . The mean bond strengths in MPa ( + /- SD ) were analyzed with two-way ANOVA and Tukey 's test ( alpha = 0.05 ) . RESULTS The application of NaOCl significantly increased the SBS values of all systems except Optibond Solo . The means ( SD ) for adhesive systems with 10 % NaOCl treatment were : SBH = 14.28 (4.21)a ; B1H = 14.39 (3.61)a ; OSH = 10.35 (5.74)a ; and values without 10 % NaOCl were : SB = 10.35 (2.95)a ; B1 = 9.38 (3.10)a ; OS = 9.79 (3.48)b . Different superscripts indicate significant difference . CONCLUSION The removal of collagen increased the strength of dentin bonding , depending on the adhesive system used
Conclusions In light of the currently available scientific evidence , dentin surface pretreatment with deproteinizing agents does not enhance the bonding of SE adhesives to dentin .
The HOCl deproteinizing agent exhibited minimal adverse effects on bonding to dentin in comparison with NaOCl solutions
Objectives The aim of this systematic review was to critically analyze previously published studies of the effects of dentin surface pretreatment with deproteinizing agents on the bonding of  self-etch ( SE ) adhesives to dentin .
Additionally , a meta- analysis was conducted to quantify the effects of the above-mentioned surface pretreatment methods on the bonding of SE adhesives to dentin .