Both synthetic and naturally derived crosslinkers have been found to exhibit significant effects in biomodification of dentine via their multiple interactions with the dentine matrix .
A stable matrix network or a durable hybrid layer in dentine bonding could be achieved , where the dentine collagen fibrils show improved biochemical and biomechanical properties and enzymatic biodegradation is reduced .
This aim of this review is to explore the current research related to  crosslinking agents used on dentine .
Biomimetic Approach for Root Caries Prevention Using a Proanthocyanidin-Rich Agent
The aim of this study was to evaluate the effects of a proanthocyanidin-rich grape seed extract ( GSE ) on the in vitro demineralization of root dentine . Root fragments were obtained from sound human teeth . The fragments were r and omly assigned to different treatments solutions : GSE , fluoride ( F ) , GSE+F and distilled water ( control ) . Sample s were treated daily for 30 min and subjected to a pH cycling artificial caries protocol using demineralization cycles ( 2.2 mM CaCl2 × H2O , 2.2 mM KH2PO4 , 50 mM acetic acid , pH 4.3 ) for 6 h and remineralization cycles ( 20 mM HEPES , 2.25 mM CaCl2 × H2O , 1.35 mM KH2PO4 , 130 mM KCl , pH 7.0 ) for 17.5 h. Mineral loss ( ΔZ ) and lesion depth ( LD ) were determined after 18 days of treatment/pH cycling , by transverse microradiography . GSE was able to minimize ΔZ and LD compared with the control group ( p < 0.0001 ) . The GSE+F and F groups showed the lowest values of ΔZ and LD ( p < 0.05 ) , with no statistically significant differences between them ( p = 0.554 and p = 0.726 , respectively ) . A biomimetic approach to strengthen root dentine using GSE results in decreased rates of root demineralization and may be used in conjunction with F to prevent root caries
Both synthetic and naturally derived crosslinkers have been found to exhibit significant effects in biomodification of dentine via their multiple interactions with the dentine matrix .
A stable matrix network or a durable hybrid layer in dentine bonding could be achieved , where the dentine collagen fibrils show improved biochemical and biomechanical properties and enzymatic biodegradation is reduced .
This aim of this review is to explore the current research related to  crosslinking agents used on dentine .