R and omized controlled trials found a positive correlation for SB with BMI , neck circumference , fat mass , HbA1C , cholesterol and insulin levels , cohort studies additionally for WC , leptin , C-peptide , ApoA1 and Low density lipoprotein and a negative correlation for HDL .
Most studied biomarkers associated with SB were of cardiovascular or metabolic origin .
OBJECTIVE Pathomechanisms of sedentary behaviour ( SB ) are unclear .
We conducted a systematic review to investigate the associations between SB and various biomarkers in older adults .
Higher mitochondrial respiration and uncoupling with reduced electron transport chain content in vivo in muscle of sedentary versus active subjects.
OBJECTIVE This study investigated the disparity between muscle metabolic rate and mitochondrial metabolism in human muscle of sedentary vs. active individuals . RESEARCH DESIGN AND METHODS Chronic activity level was characterized by a physical activity question naire and a triaxial accelerometer as well as a maximal oxygen uptake test . The ATP and O(2 ) fluxes and mitochondrial coupling ( ATP/O(2 ) or P/O ) in resting muscle as well as mitochondrial capacity ( ATP(max ) ) were determined in vivo in human vastus lateralis muscle using magnetic resonance and optical spectroscopy on 24 sedentary and seven active subjects . Muscle biopsies were analyzed for electron transport chain content ( using complex III as a representative marker ) and mitochondrial proteins associated with antioxidant protection . RESULTS Sedentary muscle had lower electron transport chain complex content ( 65 % of the active group ) in proportion to the reduction in ATP(max ) ( 0.69 ± 0.07 vs. 1.07 ± 0.06 mM sec(-1 ) ) as compared with active subjects . This lower ATP(max ) paired with an unchanged O(2 ) flux in resting muscle between groups result ed in a doubling of O(2 ) flux per ATP(max ) ( 3.3 ± 0.3 vs. 1.7 ± 0.2 μM O(2 ) per mM ATP ) that reflected mitochondrial uncoupling ( P/O = 1.41 ± 0.1 vs. 2.1 ± 0.3 ) and greater UCP3/complex III ( 6.0 ± 0.7 vs. 3.8 ± 0.3 ) in sedentary vs. active subjects . CONCLUSION A smaller mitochondrial pool serving the same O(2 ) flux result ed in elevated mitochondrial respiration in sedentary muscle . In addition , uncoupling contributed to this higher mitochondrial respiration . This finding resolves the paradox of stable muscle metabolism but greater mitochondrial respiration in muscle of inactive vs. active subjects
R and omized controlled trials found a positive correlation for SB with BMI , neck circumference , fat mass , HbA1C , cholesterol and insulin levels , cohort studies additionally for WC , leptin , C-peptide , ApoA1 and Low density lipoprotein and a negative correlation for HDL .
Most studied biomarkers associated with SB were of cardiovascular or metabolic origin .
OBJECTIVE Pathomechanisms of sedentary behaviour ( SB ) are unclear .
We conducted a systematic review to investigate the associations between SB and various biomarkers in older adults .