Both synthetic and naturally derived crosslinkers have been found to exhibit significant effects in biomodification of dentine via their multiple interactions with the dentine matrix .
A stable matrix network or a durable hybrid layer in dentine bonding could be achieved , where the dentine collagen fibrils show improved biochemical and biomechanical properties and enzymatic biodegradation is reduced .
This aim of this review is to explore the current research related to  crosslinking agents used on dentine .
Synergistic effect of proanthocyanidin and CPP-ACFP on remineralization of artificial root caries.
BACKGROUND This study evaluated the synergistic effect of proanthocyanidin ( PA ) and casein phosphopeptide amorphous calcium fluoride phosphate ( CPP-ACFP ) on remineralization of artificial root caries . METHODS Demineralized root fragments ( n = 90 ) were r and omly divided into six groups based on treatments : ( 1 ) 6.5 % PA ; ( 2 ) CPP-ACP ; ( 3 ) CPP-ACFP ; ( 4 ) CPP-ACFP + 6.5 % PA ; ( 5 ) 1000 ppm fluoride ; and ( 6 ) deionized water ( control ) . Each specimen was immersed in demineralizing solution for 14 h , testing solutions/pastes for 2 h , remineralizing solution for 8 h and pH cycling was performed at 37 ° C for 8 days . Specimens were evaluated using transverse microradiography ( lesion depth and mineral loss ) , Knoop microhardness and confocal laser scanning microscopy . RESULTS Lesion depth was significantly lower in CPP-ACFP+PA and 1000 ppm fluoride groups ( p < 0.001 ) with no significant difference between the two groups ( p > 0.05 ) . Mineral loss was the lowest in CPP-ACFP+PA group ( p < 0.05 ) . Fluoride and CPP-ACFP groups showed similar mineral gain ( p > 0.05 ) . Artificial caries lesions treated with CPP-ACFP+PA showed significantly higher microhardness values at 130 μm and 150 μm from the surface ( p < 0.05 ) . CONCLUSIONS Combined use of PA and CPP-ACFP has a synergistic effect on root caries remineralization by enhancing mineral gain and increasing hardness of artificial root caries
Both synthetic and naturally derived crosslinkers have been found to exhibit significant effects in biomodification of dentine via their multiple interactions with the dentine matrix .
A stable matrix network or a durable hybrid layer in dentine bonding could be achieved , where the dentine collagen fibrils show improved biochemical and biomechanical properties and enzymatic biodegradation is reduced .
This aim of this review is to explore the current research related to  crosslinking agents used on dentine .