Calcium hydroxide has limited effectiveness in eliminating bacteria from human root canal when assessed by culture techniques
AIM To determine to what extent does calcium hydroxide intracanal medication eliminate bacteria from human root canals , compared with the same canals before medication , as measured by the number of positive cultures , in patients undergoing root canal treatment for apical periodontitis ( teeth with an infected root canal system ) .
Reduction of intracanal bacteria using GT rotary instrumentation, 5.25% NaOCl, EDTA, and Ca(OH)2.
This study was conducted to determine the bacterial reduction using Profile GT files and a strict irrigation protocol utilizing 5.25 % NaOCl and EDTA . The additive antibacterial effect of Ca(OH)2 was also evaluated . In addition , the study compared the bacterial reduction with the GT protocol versus larger instrumentation .  Thirty-one subjects with apical periodontitis were recruited . Bacterial sample s were taken upon access ( S1 ) , after instrumentation and a strict irrigation protocol ( S2 ) , and following > 1 wk of Ca(OH)2 ( SC ) . A log10 transformation of colony forming units was done since sample bacterial counts are not normally distributed . At S1 , 93.55 % of canals sample d bacteria . At S2 , 52.72 % of the cases sample d bacteria . At SC , 14 % of the cases cultured bacteria . The McNemar test showed a significant reduction ( p<0.0009 ) in bacteria between S1 and S2 . This was also true between S2 and SC ( p<0.0019 ) . It was concluded the GT protocol significantly reduced the number of bacteria in the canal but failed to render the canal bacteria free in more than half of the cases Ca(OH)2 application significantly further reduced bacteria . Lastly , large apical instrumentation removed more bacteria than small apical instrumentation
Calcium hydroxide has limited effectiveness in eliminating bacteria from human root canal when assessed by culture techniques
AIM To determine to what extent does calcium hydroxide intracanal medication eliminate bacteria from human root canals , compared with the same canals before medication , as measured by the number of positive cultures , in patients undergoing root canal treatment for apical periodontitis ( teeth with an infected root canal system ) .