Both synthetic and naturally derived crosslinkers have been found to exhibit significant effects in biomodification of dentine via their multiple interactions with the dentine matrix .
A stable matrix network or a durable hybrid layer in dentine bonding could be achieved , where the dentine collagen fibrils show improved biochemical and biomechanical properties and enzymatic biodegradation is reduced .
This aim of this review is to explore the current research related to  crosslinking agents used on dentine .
The use of collagen cross-linking agents to enhance dentin bond strength.
UNLABELLED Type I collagen is a major component of the hybrid layer , and improvement of its mechanical properties may be advantageous during bonding procedures . OBJECTIVE To investigate the effect of three different cross-linking agents ( Glutaraldehyde [ GD ] , Grape seed extract [ GSE ] , and Genipin [ GE ] ) on the tensile bond strength ( TBS ) of resin-dentin bonds . MATERIAL S AND METHODS Sixty-four sound human molars were collected and their occlusal surfaces were ground flat to expose dentin . Dentin surfaces were etched using a phosphoric acid and then teeth were r and omly divided according to the dentin treatment : Control group ( no treatment ) , 5 % GD , 6.5 % GSE , or 0.5 % GE . Teeth were restored either with One Step Plus or Adper Single Bond Plus adhesive systems and resin composite . After 24 h , teeth were sectioned to produce a cross-sectional surface area of 1.0 mm(2 ) and tested for tensile bond strength . Data were statistically analyzed using ANOVA and Fisher 's PLSD tests ( p < 0.05 ) . There was a statistically significant interaction between factors ( treatment and adhesive p < 0.001 ) . Treatment affected TBS ( p < 0.0001 ) , while no differences were observed between the adhesive systems ( p = 0.6961 ) . CONCLUSION Chemical modification to the dentin matrix promoted by GD and GSE , but not GE , result ed in increased bond strength . The application of selective collagen cross-linkers during adhesive restorative procedures may be a new approach to improve dentin bond strength properties
Both synthetic and naturally derived crosslinkers have been found to exhibit significant effects in biomodification of dentine via their multiple interactions with the dentine matrix .
A stable matrix network or a durable hybrid layer in dentine bonding could be achieved , where the dentine collagen fibrils show improved biochemical and biomechanical properties and enzymatic biodegradation is reduced .
This aim of this review is to explore the current research related to  crosslinking agents used on dentine .