Both synthetic and naturally derived crosslinkers have been found to exhibit significant effects in biomodification of dentine via their multiple interactions with the dentine matrix .
A stable matrix network or a durable hybrid layer in dentine bonding could be achieved , where the dentine collagen fibrils show improved biochemical and biomechanical properties and enzymatic biodegradation is reduced .
This aim of this review is to explore the current research related to  crosslinking agents used on dentine .
Bonding efficacy of etch-and-rinse adhesives after dentin biomodification using ethanol saturation and collagen cross-linker pretreatment
Aim : To evaluate whether the application of two simplified etch- and -rinse adhesives to biomodified dentin using ethanol-wet bonding ( EWB ) and collagen cross-linker ( CCL ) pretreatment improves their sealing ability . Material s and Methods : In 176 extracted human molars , the pulp-chambers were deroofed , and teeth were sectioned horizontally . Sample s were r and omly divided into eight groups according to four bonding techniques using two simplified etch- and -rinse adhesives ; Adper Single Bond 2 ( ASB ) and XP Bond ( XPB ) . The bonding protocol s included : ( a ) Water-wet bonding ( WWB ) ; ( b ) EWB ; ( c ) WWB and CCL application ; ( d ) EWB and CCL application . After composite resin restorations , dye leakage evaluation and scanning electron microscope analysis were done . Leakage scores were statistically analyzed using Kruskal-Wallis and Mann-Whitney U tests at a significance level of P < 0.05 . Result : For both ASB and XPB adhesives , least dye leakage was observed in EWB groups ( b and d ) ( P = 0.918 and P = 0.399 respectively ) which showed no significant difference , while maximum leakage scores were seen in WWB groups ( a and c ) . Regardless of CCL application and adhesives used , EWB technique depicted ( P = 0.003 and P = 0.004 ) significantly greater sealing ability than WWB . Conclusion : Bonding of ASB and XPB using EWB significantly improved their sealing ability . Biomodification using CCL pretreatment had no significant effect on the sealing ability of adhesives bonded with either WWB or EWB
Both synthetic and naturally derived crosslinkers have been found to exhibit significant effects in biomodification of dentine via their multiple interactions with the dentine matrix .
A stable matrix network or a durable hybrid layer in dentine bonding could be achieved , where the dentine collagen fibrils show improved biochemical and biomechanical properties and enzymatic biodegradation is reduced .
This aim of this review is to explore the current research related to  crosslinking agents used on dentine .