Conclusion : The administration of a probiotic mixture containing L. acidophilus DDS-1 , B. lactis UABLA- 12 , and fructo-oligosaccharide was associated with significant clinical improvement in children with AD , with corresponding lymphocyte subset changes in peripheral blood .
Abstract Background : The role of probiotics in the treatment of atopic dermatitis ( AD ) remains controversial .
A recent systematic review of the available evidence called for further clinical trials with new probiotic formulations .
Objective : To assess the clinical efficacy and impact of Lactobacillus acidophilus DDS-1 , Bifidobacterium lactis UABLA-12 with fructo-oligosaccharide on peripheral blood lymphocyte subsets in preschool children with moderate-to-severe AD .
Development of allergen-specific T-cell memory in atopic and normal children
BACKGROUND In the past 20 - 30 years , there has been an increase in prevalence of allergic respiratory diseases , particularly amongst children . This study is a prospect i ve analysis of the postnatal maturation of T-helper cell ( Th ) responses to aeroallergens in atopic and non-atopic infants . METHODS We measured mononuclear-cell proliferative and cytokine responses to specific allergens and tetanus toxoid in blood sample s from atopic and non-atopic infants every 6 months from birth to 2 years of age . Cytokine analyses of responses to housedust-mite allergen used ELISA and reverse-transcriptase PCR . We also measured responses to Fel d1 ( cat allergen ) and tetanus toxoid . FINDINGS Sample s from 18 atopic and 13 non-atopic infants showed low-level Th2-skewed allergen-specific responses at birth , with little accompanying specific interferon-gamma production . Neonatal Th2 responses were lower in the atopic group than in the non-atopic group ; the differences were significant for interleukin-4 ( mRNA : beta-actin ratio 0.48 [ SE 0.15 ] vs 0.15 [ 0.06 ] , p=0.049 ) , interleukin-6 ( 4750 [ 48 ] vs 1352 [ 51 ] pg/mL culture fluid , p=0.003 ) , interleukin-10 ( 1162 [ 228 ] vs 485 [ 89 ] , p=0.015 ) , and interleukin-13 ( 7.1 [ 0.9 ] vs 0.9 [ 0.3 ] , p=0.008 ) . There was rapid suppression of Th2 responses during the first year of life in non-atopic children , but there was consolidation of responses in atopic children , associated with defective neonatal interferon-gamma production . INTERPRETATION The continuation of fetal allergen-specific Th2 responses during infancy is a defining feature of the inductive phase of atopic disease , and is associated with decreased capacity for production of the Th1 cytokine interferon y by atopic neonates . These findings provide a plausible mechanism for persistence of the fetal Th2 responses during early childhood in atopic individuals and subsequent expression of disease
Conclusion : The administration of a probiotic mixture containing L. acidophilus DDS-1 , B. lactis UABLA- 12 , and fructo-oligosaccharide was associated with significant clinical improvement in children with AD , with corresponding lymphocyte subset changes in peripheral blood .
Abstract Background : The role of probiotics in the treatment of atopic dermatitis ( AD ) remains controversial .
A recent systematic review of the available evidence called for further clinical trials with new probiotic formulations .
Objective : To assess the clinical efficacy and impact of Lactobacillus acidophilus DDS-1 , Bifidobacterium lactis UABLA-12 with fructo-oligosaccharide on peripheral blood lymphocyte subsets in preschool children with moderate-to-severe AD .