Question: Iowa Highway 394 was replaced by Iowa 27 and is now part of which 563 mi highway in the Midwestern United States?

Context paragraphs:
Lincoln Highway Bridge (Tama, Iowa): The Lincoln Highway Bridge is located in Tama, Iowa, United States, along the historic Lincoln Highway.  The Steel stringer bridge was built in 1914, and it was listed on the National Register of Historic Places in 1978.  It spans Mud Creek for 22 ft with a width of 24 ft .  The bridge is noteworthy for its distinctive railings.  They are 3.33 ft high, and feature the words "Lincoln Highway" in concrete.  Local boosters of the Lincoln Highway paid for the railings as a way of promoting the roadway.  The bridge was designed by the Iowa Highway Commission and built by Paul N. Kingsley, a contractor from Strawberry Point, Iowa.

Iowa Highway 136: Iowa Highway 136 (Iowa 136) is a state highway maintained by the Iowa Department of Transportation, which runs for 98 mi in eastern Iowa.  It begins at the Mississippi River in Clinton on the Mark Morris Memorial Bridge, where it continues as Illinois Route 136.  It ends at an intersection with U.S. Route 52 and Iowa Highway 3 in Luxemburg.  Although signed as a north–south highway throughout, the highway runs east to west between Clinton and Oxford Junction, while turning north at Oxford Junction to complete its run to Luxemburg.  For most of its existence, Iowa 136 has connected Clinton and Luxemburg.  During the 1950s-1960s, the northern and southern ends of the route shifted slightly because nearby United States highways changed their alignments.

Avenue of the Saints: The Avenue of the Saints is a 563 mi highway in the Midwestern United States that connects St. Paul, Minnesota and St. Louis, Missouri.

Iowa Highway 150: Iowa Highway 150 (Iowa 150) is an 85 mi state highway in eastern and northeastern Iowa.  It begins at U.S. Route 218 (US 218) in Vinton and ends at US 52 and Iowa 24 in Calmar.  From Vinton, it heads north and east towards Urbana where it meets Interstate 380 (I-380) and Iowa 27, the Avenue of the Saints highway.  At Independence, it intersects US 20 on the south side of town.  Further north, it converges with Iowa 3 in Oelwein.

Iowa Highway 139: Iowa Highway 139 (Iowa 139) is an 11 mi highway in northeastern Iowa.  It begins at Iowa 9 east of Cresco and ends at the Minnesota state line, where it continues northward as Minnesota State Highway 139.  It crosses the Upper Iowa River near Kendallville.  The roadway has been a part of the state's primary highway system since its inception in 1920; however, it has only been known as Iowa 139 since 1930.  From 1935 to 1980, the highway extended south to Protivin.

Interstate 35 in Iowa: Interstate 35 (I-35) is a north–south Interstate Highway in the United States that runs from Laredo, Texas, to Duluth, Minnesota.  In Iowa, the highway runs from south to north through the center of the state, roughly parallel to U.S. Highway 69 (US 69) and US 65.  It enters the state near Lamoni from Missouri and continues north through the southern Iowa drift plain.  In the Des Moines area, I-35 is overlapped by I-80 and the two routes bypass Des Moines to the west and north.  I-235, the only auxiliary route of I-35, serves the suburbs and downtown Des Moines; it begins and ends at the two interchanges where I-35 and I-80 meet.  Near Mason City and Clear Lake, US 18 and Iowa Highway 27 (Iowa 27) overlap with I-35.  Shortly after, US 18 splits off to the west in Clear Lake, while Iowa 27 runs with I-35 until they reach the Minnesota state line.

Mark Morris Memorial Bridge: The Mark Morris Memorial Bridge (locally called the North Bridge) is a 2 lane truss bridge across the Mississippi River in the United States.  It connects the cities of Clinton, Iowa and Fulton, Illinois.  The bridge may also be known as the Lyons-Fulton Bridge, which was the name of a predecessor bridge and the name listed on the USGS topographical map.  The town of Lyons, Iowa, was annexed to Clinton in 1895, but the north end of the city is still referred to as Lyons.  The bridge is the terminus of both Iowa Highway 136 and Illinois Route 136.  The 1975 bridge was named in memory Mark Morris, a long time member of the City of Clinton Bridge Commission who died in 1972.  Morris was instrumental in the construction of the 1975 bridge and the City of Clinton Bridge Commission named it in his honor.  The bridge was opened in January 1975, replacing an older span upstream that once carried the Lincoln Highway, U.S. Route 30.)  In 1982, Iowa DOT announced that it would be removing a 20 cent toll from the Gateway Bridge and the Mark Morris bridge beginning January 1983.  Iowa and Illinois agreed to split responsibility for the maintenance of the two bridges with Iowa maintaining the Mark Morris bridge and Illinois the Gateway bridge.

Iowa Highway 15 (Van Buren County): Iowa Highway 15 (Iowa 15) was a short state highway in Van Buren County.  It ran from the Missouri state line, where it connected to Route 15 to Iowa 2 in Milton.  The highway was designated on January 1, 1969, when the Iowa State Highway Commission renumbered several state highways in order to match up with adjoining highways in neighboring states.  The route ceased to exist on July 1, 2003, when the Iowa Department of Transportation turned over 700 mi of state highways that mostly served local traffic.  While it was in service, there were two Iowa 15s in the state; the other was significantly longer and located in the northern part of the state thus avoiding confusion between the two routes.

Iowa Highway 394: Iowa Highway 394 (Iowa 394) was a state highway in Lee County in southeastern Iowa.  It began on a toll bridge to St. Francisville, Missouri, over the Des Moines River and ended at U.S. Highway 218 (US 218) south of Donnellson.  The highway was in service from 1980 to 2005.  It was replaced by Iowa 27 as part of the larger Avenue of the Saints corridor project.

U.S. Route 34 in Iowa: U.S. Highway 34 (US 34) is a United States Highway that runs across the southern third of Iowa.  It begins on a bridge over the Missouri River west of Glenwood and travels east where it meets Interstate 29 (I-29) and US 275.  Through southwestern Iowa, the highway is, for the most part, a two-lane rural road.  Its interchanges with US 59 near Emerson and US 71 near Stanton and Villisca are located away from populated areas.  At Osceola, the highway intersects I-35 and US 69.  Just east of Ottumwa, where the road meets US 63, the road widens four lanes for the remainder of its trek through the state.  At Mount Pleasant, it overlaps US 218 and Iowa 27, the Avenue of the Saints Highway.  From there, the road heads to the southeast where it crosses the Mississippi River on the Great River Bridge at Burlington.

Supporting paragraphs: Iowa Highway 394 Avenue of the Saints
Answer: Avenue of the Saints