Patent SG-11201805600V-A (SG)
Title: Deep sequencing profiling of tumors
Inventor: ALEXANDER NELSON
IPC: C12Q1/68
Filed: 2016-11-07
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization MD 1111101110101011111 HO 111110111011101111011011 OH III MEV III International Bureau 0.. .... .. (10) International Publication Number (43) International Publication Date ..... ...r .....1 WO 2017/123316 Al 20 July 2017 (20.07.2017) WIPO I PCT (51) (21) (22) (25) Filing Language: (26) Publication Language: (30) International Patent Classification: (71) Applicants (for US only): VENTANA MEDICAL SYS- C12Q 1/68 (2006.01) TEMS, INC. [US/US]; 1910 E. Innovation Park Dr., Tuc- International Application Number: son, Arizona 85755 (US). ROCHE SEQUENCING PCT/US2016/060835 SOLUTIONS, INC. [US/US]; 4300 Hacienda Drive, Pleasanton, California 94588 (US). International Filing Date: (71) Applicants (for DE only): VENTANA MEDICAL SYS- 7 November 2016 (07.11.2016) TEMS, INC. [US/US]; 1910 E. Innovation Park Dr., Tuc- English son, Arizona 85755 (US). ROCHE DIAGNOSTICS GMBH [DE/DE]; Sandhofer Strasse 116, 68305 Man- English nheim (DE). Priority Data: (71) Applicants o r all designated States except DE, US): 62/279,126 15 January 2016 (15.01.2016) US VENTANA MEDICAL SYSTEMS, INC. [US/US]; 1910 62/415,952 1 November 2016 (01.11.2016) US E. Innovation Park Dr., Tucson, Arizona 85755 (US). F. HOFFMANN-LA ROCHE AG [CH/CH]; Grenzacher- strasse 124, 4070 Basel (CH). (72) Inventors: ALEXANDER, Nelson; 11440 N. Vista Ranch Place, Marana, Arizona 85658 (US). BURGESS, Daniel; [Continued on next page] (54) Title: DEEP SEQUENCING PROFILING OF TUMORS (57) such 300 sequencing. 310 320 330 340 . targeted prising : In one aspect of the present disclosure is a sequencing workflow where an input sample com- a sufficient quantity of genomic material is provided minimal or no amplification cycles are utilized prior to Receive a sufficient quantity of genomic materi al Prepare received material for further processing Hybridize genomic material to capture probes Capture hybridized genomic material Sequence hybridized genomic material 1-1 .4 11 M M ei 1-1 IN 1-1 0 ei C FIG. 3A WO 2017/123316 All#110H011010EIR0101301101110110MHIMM0N 500 S. Rosa Road, Madison, Wisconsin 53719 (US). STANISLAW, Stacey; 9971 Rabwa Place, Tucson, Ari- zona 85742 (US). ROSENBAUM, Heidi; 2303 May- SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. wood Circle, Middleton, Wisconsin 53562 (US). (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (74) (81) Agent: FINETTI, Thomas; Charney IP Law LLC, 233 Mount Airy Road, Suite 100, Basking Ridge, New Jersey 07920 (US). Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, Declarations under Rule 4.17: GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, Published: RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, with international search report (Art. 21(3))