Patent SG-11201900447S-A (SG)
Title: Methods and compositions for modifying genomic dna
Inventor: LI LINHONG
IPC: C12N15/90
Filed: 2017-07-21
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) - (19) World Intellectual Property 11111111111011101 HIM 011010011111111111 1111111111 11111 1111111E1111 0111111 Organization - - ' International Bureau (10) International Publication Number (43) International Publication Date .....0\"\" WO 2018/015936 A3 25 January 2018 (25.01.2018) WI P0 I P C T (51) International Patent Classification: C12N 5/10 (2006.01) Cl 2N 15/82 (2006.01) Madhusudan; c/o MaxCyte, Inc., 22 Firstfield Road, Suite 250, Gaithersburg, Maryland 20878 (US). C12N 9/22 (2006.01) Cl 2N 15/90 (2006.01) (74) Agent: STEELMAN, Laurie et al.; NORTON ROSE (21) International Application Number: FULBRIGHT US LLP, 98 San Jacinto Blvd., Suite 1100, PCT/IB2017/054446 Austin, Texas 78701 (US). (22) International Filing Date: (81) Designated States (unless otherwise indicated, for every 21 July 2017 (21.07.2017) kind of national protection available): AE, AG, AL, AM, (25) Filing Language: English AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (26) Publication Language: English DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (30) Priority Data: HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, 62/365,126 21 July 2016 (21.07.2016) US KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (71) Applicant: MAXCYTE, INC. [US/US]; 22 Firstfield OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, Road, Suite 250, Gaithersburg, Maryland 20878 (US). SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (72) Inventors: LI, Linhong; c/o MaxCyte, Inc., 22 First- field Road, Suite 250, Gaithersburg, Maryland 20878 (US). (84) Designated States (unless otherwise indicated, for every ALLEN, Cornell; c/o MaxCyte, Inc., 22 Firstfield Road, kind of regional protection available): ARIPO (BW, GH, _ Suite 250, Gaithersburg, Maryland 20878 (US). PESHWA, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, = = Title: METHODS AND COMPOSITIONS FOR MODIFYING GENOMIC DNA = (54) = = F Traplecti Stablel )creP1 ni,g( ex p ki: - rt —:poo , scale-up 0 * = li len 1 i rr. th .. be , :': ci ::nc4.:(s) LOC 1.6 .lt fCi 3 ::eiVNeit) pli2se — !r1 CD C. HO 7 ‘; ' - W• ..., = _ = s e l ec tion Mairter5a31cef ckne s.c.reeMrsg phi s i Ciclial 5electiott 44z e7Tarisic:4 phase phase Abst ' :ACC(.-SS6C1 = = = _ = III Oar* = IA days 14 days 1t .days 1440: days >:tqtair.? ire* M .4 FIG. 1 en cN (57) : Compositions and methods concern the sequence modification of an endogenous genomic DNA region. Certain aspects 11) relate to a method for site-specific sequence modification of a target genomic DNA region in cells comprising: transfecting the cells 1-1 by electroporation with a composition comprising (a) a DNA oligo; (b) a DNA digesting agent; and (c) a targeting RNA, wherein © - --- GC the targeting RNA is capped and/or polyadenylated; wherein the donor DNA comprises: (i) a homologous region comprising nucleic acid sequence homologous to the target genomic DNA region and (ii) a sequence modification region; and wherein the genomic DNA 0 sequence is modified specifically at the target genomic DNA region. ei O [Continued on next page] WO 2018/015936 A3 MIDEDIMOMOIDEIREEMOOMMOMMOROMEfin TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) — with sequence listing part of description (Rule 5.2(a)) (88) Date of publication of the international search report: 01 March 2018 (01.03.2018)