Patent AU-2021102741-A4 (AU)
Title: A novel methodology for the production and inoculation of periodontal ligament stem cells from periodontal ligament fibers on titanium implants in an animal model
Inventor: JHA KUKREJA BHAVNA
IPC: C12N5/0775
Filed: 2021-05-21
Granted: 2022-03-24
Abstract: Human tissues have been designed uniquely. In the current times, there has arisen another and 
aggressive approach that joins information from material science with cell science and 
medication. These are called realms of tissue engineering science. These techniques have utilized 
biodegradable polymers to make frameworks into the cells, embedded to create tissues within the 
sight of development factors. In a human body a single embryonic stem cell can be differentiate 
into specialized 220 cell types. However, embryonic stem cells Introduction has a high potential 
for causing tumours; making them less desirable to use than adult stem cells. With effective 
minimally invasive procedures, the progenitor cells in periodontal tissue has an extensive version 
as vitro, offers a singular pool of stem cells. They are a viable alternative to bone marrow 
aspiration, as procurement of PDLSCs from premolars is the less invasive and morbid procedure 
with minimal complications. The present study findings suggest that PDLSCs can be isolated and 
cultured from periodontal ligament cells of extracted adult premolars by using CD45, CD73, 
CD90, CD105 and CD146 markers. The current work elicits that CD 146, CD 105, CD 90, CD 
73 is a reliable positive marker for the identification of periodontal ligament stem cells and CD 
45 is a reliable negative marker for the identification of periodontal ligament stem cells.  
Periodontal formation occurs during cultured PDLSCs on the dedicated surface of titanium, 
which can be confirmed both histologically and radiologically. It can safely be postulated that 
from the present study that tissue engineering of the PDL has been achieved as a proof-of
concept in present study. We have observed important intertissued interactions like the formation 
of a functional PDL around the implantation site, and induction of the formation of bone in the 
vicinity of the implants. Ligaplants as tooth substitutions may have unequivocal focal points as 
contrasted and osseointegrated gadgets, because of their potential for periodontal tissue recovery.  
It is recommended that restorative achievement requires the capacity of a high extent of the 
developed cells to coordinate into another PDL. The ligaplant medical procedure is moderately 
simple because the embed isn't firmly fitted into its site. Future clinical utilization of ligaplants 
may stay away from bone uniting with its cost, bother, and uneasiness for the patient. The 
bioengineering of clinical implants of PDL would be tremendous alternative for stable and 
natural tooth implant therapy.  
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Sample Images 
Figure-4: Positive Staining for CD90 (20X 
Figure-i:Scraping ofPDLcellsfromextracted magnification) 
teeth 
B C 
Figure-2: Colonies of PDLSCs formed from Figure-5: Positive Staining for CD105 (20X 
isolated PDLSCs at 14 days magnification) 
D 
Figure-3: Positive Staining for CD73 (20X Figure-6: Positive Staining for CD146 (20X 
magnification) magnification) 
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