Patent SG-11201907812T-A (SG)
Title: Production of carotenoids and apocarotenoids
Inventor: ZHANG CONGQIANG
IPC: C12P23/00
Filed: 2018-02-26
Abstract: FIGAPOCAROTENOIDc - a-lonone (C13) retinal (c20) v 4 ..„ / ipnone (C13) ROTENES siese , ca.Fo , •: diakiehyde (4:17) dialdehyde (C19) trans-geranylaretone (C13) f a-. t . 0-cyclocitrai KW} 1-1 GC O kr) 00 O 1-1 1-1 N C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 30 August 2018 (30.08.2018) omit n OH iolo 1101100 IIMIHREMO 011 (10) International Publication Number WO 2018/156086 Al WIPO I PCT (51) International Patent Classification: C12P 23/00 (2006.01) Cl 2N 15/52 (2006.01) C12P 5/00 (2006.01) (21) International Application Number: PCT/SG2018/050087 (22) International Filing Date: 26 February 2018 (26.02.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 10201701500U 24 February 2017 (24.02.2017) SG (71) Applicant: AGENCY FOR SCIENCE, TECHNOLO- GY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, Connexis North Tower, #20-10, Singapore 138632 (SG). (72) Inventors: ZHANG, Congqiang; c/o Biomedical Sciences Institute, Biotransformation Innovation Platform (Bio- Trans), 61 Biopolis Drive, Proteos, #04-14, Singapore 138673 (SG). CHEN, Xixian; c/o Biomedical Sciences In- stitute, Biotransformation Innovation Platform (BioTrans), 61 Biopolis Drive, Proteos, #04-14, Singapore 138673 (SG). TOO, Heng-Phon; c/o Bioprocessing Technology Institute, 20 Biopolis Way, Centros, #06-01, Singapore 138668 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531, Robinson Road Post Office, Singapore 903031 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (54) Title: PRODUCTION OF CAROTENOIDS AND APOCAROTENOIDS Psi-ionone (C1.3) 6-Methyl-5 hepten-2- one (MHO, C8) (57) : A method for producing a carotenoid and apocarotenoid is disclosed. The method comprises the step of expressing in a host cell an expression module comprising an expression vector having a coding region encoding at least one optimised carotenoid or apocarotenoid generating enzyme, the coding region being operably linked to a promoter. A host cell comprising an expression vector having a coding region encoding at least one optimised carotenoid or apocarotenoid generating enzyme, the coding region being operably linked to a promoter is also provided together with a kit. In particular, the carotenoid or apocarotenoid generating enzymes form an operon selected from: a. AN50-LsLcyE and TrxA-Osmanthus fragrans CCD1 for the production of ct-ionone; or b. crtY and phCCD1 for the production of 13-ionone; or c. 41N50-LsLcyE for the production of c-carotene; or d. crtY and blh for the production of retinal; or e. crtY, blh and ybbO for the production of retinol. [Continued on next page] WO 2018/156086 Al O II HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17 (iv)) Published: with international search report (Art. 21(3)) in black and white; the international application as filed contained color or greyscale and is available for download from PATENTSCOPE