Patent SG-11201807336R-A (SG)
Title: Protein a binding polypeptides, anti-epha2 antibodies and methods of use thereof
Inventor: MARKS JAMES D
IPC: C07K16/46
Filed: 2017-03-13
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization M1111101110101011111 HO 11111 01110111100101110111110 111110111011# 11 International Bureau 0.. .... .. ..... ..or::,„, (10) International Publication Number (43) International Publication Date WO 2017/160775 Al 21 September 2017 (21.09.2017) WIP0 I PCT (51) International Patent Classification: (74) Agent: FRANCIS, Carol L.; Bozicevic, Field & Francis A61K 39/395 (2006.01) C07K 16/46 (2006.01) LLP, 201 Redwood Shores Pkwy., Suite 200, Redwood C07K 16/18 (2006.01) City, California 94065 (US). (21) International Application Number: (81) Designated States (unless otherwise indicated, for every PCT/US2017/022188 kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (22) International Filing Date: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, 13 March 2017 (13.03.2017) DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (25) Filing Language: English HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, (26) Publication Language: English MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, (30) Priority Data: NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, 62/309,365 16 March 2016 (16.03.2016) US RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, 62/309,383 16 March 2016 (16.03.2016) US TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, 62/309,374 16 March 2016 (16.03.2016) US ZA, ZM, ZW. (71) Applicants: THE REGENTS OF THE UNIVERSITY (84) Designated States (unless otherwise indicated, for every OF CALIFORNIA [US/US]; 1111 Franklin Street, kind of regional protection available): ARIPO (BW, GH, Twelfth Floor, Oakland, California 94607 (US). MER- GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, RIMACK PHARMACEUTICALS, INC. [US/US]; One TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, Kendall Square, Building 700, Suite B7201, Cambridge, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, Massachusetts 02139 (US). DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, — LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, _ (72) Inventors: MARKS, James D.; 107 Ardmore Road, Kens- SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, ington, California 94707 (US). DRUMMOND, Daryl; 1 GW, KM, ML, MR, NE, SN, TD, TG). = Brooks Road, Lincoln, Massachusetts 01773 (US). GED- DIE, Melissa; 28 Brand Street, Arlington, Massachusetts Published: 02474 (US). KIRPOTIN, Dmitri; 382 Ocean Avenue, — with international search report (Art. 21(3)) Apt. 1509, Revere, Massachusetts 02151 (US). KOHLI, before the expiration of the time limit for amending the Neeraj; 7 Wheaton Road, Arlington, Massachusetts 02474 claims and to be republished in the event of receipt of (US). LUGOVSKY, Alexey; 35 Audrey Road, Belmont, amendments (Rule 48.2(h)) = — Massachusetts 02478 (US). = — with sequence listing part of description (Rule 5.2(a)) = = = (54) Title: PROTEIN A BINDING POLYPEPTIDES, = 80— = 2 _ ce _ a = E 60 '2 — 2, 40— _ co fo 3 11 15 20— kin -E IN . IN © % / / /, / / ,,, • a .„.•,. ANTI-EPHA2 ANTIBODIES iii , 0 r ( / /,;.. 7 ., AND METHODS OF USE THEREOF VH: TS1 F5 F5 TS1 A7 A7 TS1 F5 A7 1-1 FIG. 1 VL: TS1 A7 TS1 TS1 TS1 F5 A7 F5 A7 IN 1-1 (57) : Provided by the present disclosure are antibodies (e.g., scFvs) that include CDRs and human framework regions that 0 confer useful properties upon the antibodies. In certain embodiments, such properties include thermostability (e.g., increased melting \" temperature), efficient binding to Staphylococcus aureus Protein A, or both. In certain aspects, the antibodies are internalizing anti - 0 bodies that specifically bind to the tumor associated antigen EphA2.