Patent SG-11201908657V-A (SG)
Title: Engineered gram-negative endolysins
Inventor: GRIESSL MARTIN
IPC: C12N9/52
Filed: 2018-04-02
Abstract: WO 18/ 185 63 4 Al (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 11 October 2018 (11.10.2018) WIPO I PCT omit °noI0 OHIIIIIIIIII1011IIIIIIIII111111 0IIIIII (10) International Publication Number WO 2018/185634 Al (51) International Patent Classification: C12N 9/52 (2006.01) (21) International Application Number: PCT/IB2018/052253 (22) International Filing Date: 02 April 2018 (02.04.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: PCT/IB2017/051886 03 April 2017 (03.04.2017) IB (71) Applicant: SASINAPAS CO., LTD. [TH/TH]; 152 North Sathorn Road, Silom, Bangrak, Bangkok, 10500 (TH). (72) Inventors: GRIESSL, Martin; Scambachring 7, 93155 Hohenschambach (DE). BIEBL, Manfred; Am Di- etlmeierhof 10, 93083 Obertraubling (DE). (74) Agent: CHANGCHUMNI, Manoon et al.; Rouse & Co. International (Thailand) Ltd., Unit 1401-3 and 1408, Floor 14, Two Pacific Place, 142 Sukhumvit Road, Klongtoey, Bangkok, 10110 (TH). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) (54) Title: ENGINEERED GRAM-NEGATIVE ENDOLYSINS (57) : The present invention relates in general to the field of antimicrobial enzymes. In particular, the present invention relates to a polypeptide comprising the amino acid sequence of a globular Gram-negative endolysin and the amino acid se- quence of a cell wall binding domain of i) a modular Gram-negative endolysin or ii) a bacteriophage tail/baseplate protein. The present invention relates also to correspond- ing nucleic acids, vectors, bacteriophages, host cells, and compositions. The present inventions also relates to the use of said polypeptides, nucleic acids, vectors, bacte- riophages, host cells, and compositions in methods for treatment of the human or an- imal body by surgery or therapy or in diagnostic methods practiced on the human or animal body. The polypeptides, nucleic acids, vectors, bacteriophages, host cells, and compositions according to the invention may also be used as an antimicrobial in, e.g., food or feed, in cosmetics, or as disinfecting agent. Fig. 1 [Continued on next page] WO 2018/185634 Al MIDEDIMOMMIDEFIEOMEIMMIENHEMOVOIS Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a)) in black and white; the international application as filed contained color or greyscale and is available for download from PATENTSCOPE