Patent SG-11201903557S-A (SG)
Title: Methods for screening b cell lymphocytes
Inventor: PARK MINHA
IPC: C12Q1/68
Filed: 2017-10-23
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1111111011111111101 IIIIIIIII101111101011H011111110011111111111111111111111110111111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\" WO 2018/076024 A2 26 April 2018 (26.04.2018) WIP0 I PCT (51) International Patent Classification: NANI, Ravinder, K. [IN/US]; 5858 Horton Street, Suite GO1N 33/574 (2006.01) C12Q 1/68 (2006.01) 320, Emeryville, CA 94608 (US). CHIRRA DINAKAR, (21) International Application Number: Hariharasudhan [IN/US]; 5858 Horton Street, Suite 320, PCT/US2017/057926 Emeryville, CA 94608 (US). SZETO, Kai, W. [US/US]; 5858 Horton Street, Suite 320, Emeryville, CA 94608 (22) International Filing Date: (US). HIGA, Adrienne, T. [US/US]; 5858 Horton Street, 23 October 2017 (23.10.2017) Suite 320, Emeryville, CA 94608 (US). WHITE, Mark, P. [US/US]; 5858 Horton Street, Suite 320, Emeryville, (25) Filing Language: English CA 94608 (US). LOWE, Randall, D. [US/US]; 5858 (26) Publication Language: English Horton Street, Suite 320, Emeryville, CA 94608 (US). (30) Priority Data: WANG, Xiaohua [CN/US]; 8203 Overhill Dr., Pomona, NY 10970 (US). CHAPMAN, Kevin, T. [US/US]; 5858 62/411,690 23 October 2016 (23.10.2016) US Horton Street Suite 320, Emeryville, CA 94608 (US). 62/412,092 24 October 2016 (24.10.2016) US (81) Designated States (unless otherwise indicated, for every (71) Applicant: BERKELEY LIGHTS, INC. [US/US]; 5858 kind of national protection available): AE, AG, AL, AM, Horton Street, Suite 320, Emeryville, CA 94608 (US). AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (72) Inventors; and CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (71) Applicants: PARK, Minha [US/US]; 5858 Horton Street, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, Suite 320, Emeryville, CA 94608 (US). BRIGGS, Jason, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, _ C. [US/US]; 5858 Horton Street, Suite 320, Emeryville, CA KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 94608 (US). MCEWEN, Jason, M. [US/US]; 5858 Hor- MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, = ton Street, Suite 320, Emeryville, CA 94608 (US). RAME- OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, = (54) Title: METHODS FOR SCREENING B CELL LYMPHOCYTES — = = = = _ _ = = _ ,1 , ,, , t• = _ ,,, = = = \ = _ = ‘ ei ii- ei FIG. 6D © IN c: : :: , (57) : Methods are described herein for screening an antibody producing cell within a microfluidic environment. The antibody -- --- GO producing cell may be a B cell lymphocyte, which may be a memory B cell or a plasma cell. An antigen of interest may be brought into proximity with the antibody producing cell and binding of the antigen by an antibody produced by the antibody producing cell may be 0 monitored. Methods of obtaining a sequencing library from an antibody producing cell are also described. ei O [Continued on next page] WO 2018/076024 A2IMIOHOIMIREIH00 IIIIIMEIRIMME110IS SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — without international search report and to be republished upon receipt of that report (Rule 48.2(g))