Patent DE-102006058730-A1 (DE)
Title: Modular three-dimensional cell culture carrier useful in biological and medical applications, comprises a compound of stacked material layers, which are perforated as non-planar single layers
Inventor: KLINKENBERG ERNST-DIETER
IPC: C12M1/40
Filed: 2006-12-13
Abstract: The modular three-dimensional cell culture carrier useful in biological and medical applications, comprises a compound of stacked material layers, which are perforated as non-planar single layers (1, 2), so that the perforations in stack compound form regular or irregular channels (4) with vertical and horizontal process portions by a stack (3). The channels are designed, so that the interconnecting porosity of a naturally interconnected porous cell culture carrier is reproduced by its process. The cell cultural carrier is implemented as medical implant. The modular three-dimensional cell culture carrier useful in biological and medical applications, comprises a compound of stacked material layers, which are perforated as non-planar single layers (1, 2), so that the perforations in stack compound form regular or irregular channels (4) with vertical and horizontal process portions by a stack (3). The channels are designed, so that the interconnecting porosity of a naturally interconnected porous three-dimensional cell culture carrier is reproduced by its process. The cell cultural carrier is implemented as medical implant. A variable geometry of the channels influences the cell growth and the cell function. The cell culture carrier flows through vertically and horizontally and forms a structure. The single layers consist of different materials and form a periodic sequence of different materials. The single layers are additionally provided with very thin layers in comparison with the characteristic thickness, are arranged irregularly or regularly, consist of electrically conductive material, electrically insulate the conductive layers against each other and are used as electrodes during contacting. A local electrical field is produced by the electrodes in the cell culture carrier structure. A local pH-value in the structure filled with a cell culture medium is influenced by the electrical field within the cell culture carrier. The cell functions are stimulated or suppressed by the local influence of the pH value in the cell culture carrier. The electrodes have geometry, so that it forms a condenser. The electrode is formed in meander-form, so that it heats itself with a current flow through the characteristic resistance and works as local heating. Two electrodes are formed as Peltier element for local cooling in the cell culture carrier structure. The electrodes are doubly contacted and formed, so that it form a magnetic field controllable over the current flow. The same or different active substances in the cell culture carrier are eluted from a thin layer or several thin layers in contact with the liquid medium. The single layers are dissolved and/or materially converted by the metabolism of the settling cells. The single layers consist of different metals, which form several electro-chemical elements in contact with the liquid medium. The adjacent layers of the single layers have chemical reactions at an interface.