Patent SG-11201811460T-A (SG)
Title: Protective surface coatings for flow cells
Inventor: RAMIREZ SEAN M
IPC: B01L3/00
Filed: 2018-05-02
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1 MI 11111 0 11101 HOE HI 1 0 01010 IIMMI11011011 OE 11110 MAMIE Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\"\" WO 2018/208561 Al 15 November 2018 (15.11.2018) WIPO I PCT (51) International Patent Classification: BOIL 3/00 (2006.01) (21) International Application Number: (22) International Filing Date: 02 (25) Filing Language: (26) Publication Language: (30) Priority Data: 62/504,977 11 May 2017 (11.05.2017) (71) Applicants: ILLUMINA, INC. na Way, San Diego, California 92122 CAMBRIDGE LIMITED [GB/GB]; Nr, Saffron Walden, Essex CB10 NA SINGAPORE PTE. LTD. [SG/SG]; #09-05, Helios 138667 (SG). (72) Inventors: RAMIREZ, Sean M.; sex CB10 1XL (GB). TRAN, Hai Quang; 5200 Illumi- na Way, San Diego, California 92122 (US). ZIMMER- LEY, Maxwell; 5200 Illumina Way, San Diego, California 92122 (US). MORRISON, Julia; Little Chesterford, Nr, PCT/US2018/030689 Saffron Walden Essex CB10 1XL (GB). ARTIOLI, Gian- luca Andrea; Little Chesterford, Nr, Saffron Walden Essex May 2018 (02.05.2018) CB10 1XL (GB). SLY, Krystal; 5200 Illumina Way, San Diego, California 92122 (US). BLACK, Hayden; 5200 Il- English lumina Way, San Diego, California 92122 (US). KRAFT, English Lewis J.; 5200 Illumina Way, San Diego, California 92122 (US). XIE, Hong; 11 Biopolis Way #09-05, Helios 138667 (SG). WEI, Wei; 5200 Illumina Way, San Diego, California US 92122 (US). SANFORD, Ryan; 5200 Illumina Way, San [US/US]; 5200 Illumi- Diego, California 92122 (US). (US). ILLUMINA (74) Agent: DIERKER, Julia Church et al.; Dierker & Ka- Little Chesterford, vanaugh, P.C., 3331 W. Big Beaver Road, Suite 109, Troy, 1XL (GB). ILLUMI- Michigan 48084 (US). 11 Biopolis Way (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, 5200 Illumina Way, San AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, Brian D.; 5200 CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, 92122 (US). LI, Ed- DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, California 92122 HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, Way, San Diego, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, Daniel; 5200 I- llu MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, 92122 (US). RICHEZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, Nr, Saffron Walden Es SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, Ludovic; 5200 Illumi-TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (US). VON HAT- Nr, Saffron Walden Es- Diego, California 92122 (US). MATHER, — = Illumina Way, San Diego, California win; 5200 Illumina Diego, Way, San (US). MOON, Sojeong; 5200 Illumina _ California 92122 (US). KIM, Innsu = mina Way, San Diego, California ego, = Alexandre; Little Chesterford, sex CB10 1XL (GB). VINCENT, _ na Way, San Diego, California 92122 TEN, Xavier; Little Chesterford - = Title: PROTECTIVE COATINGS FOR FLOW CELLS a first chemical group; a functionalized polymer polymer coating layer by a water-soluble protective examples of the water-soluble (54) SURFACE = = /00--,, \ = = Adding Surface Chemistry to a Portion of a Flow Cell Substrate = 102-- = _ = — _ = 104--Ap plying a Water-Soluble Protective Coating on at least the Surface Chemistry includes modifying an exposed surface of a substrate to incorporate a first reactive group of a functionalized polymer molecule to form surface of the substrate; grafting a primer to the functionalized group of the functionalized polymer coating layer; and forming polymer coating layer. Examples of flow cells incorporating 1-1 Il in (57) : An example of a method op reacting the first chemical group with N coating layer covalently bound to the exposed ---- reacting the primer with a second reactive GC coating on the primer and the functionalized Il © protective coating are also disclosed herein. ei O [Continued on next page] WO 2018/208561 Al MIDEDIM011010EIRDERI001010IRH11111101111111111111111111111 (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — as to the identity of the inventor (Rule 4.17(0) Published: — with international search report (Art. 21(3))