Patent GB-786396-A (GB)
Title: Aqueous vitamin compositions containing vitamin c and possessing vitamin b activity
Filed: 1956-01-02
Abstract: The preparation of a Vitamin B12-hog stomach protein complex is described in Example (1).  Hog pyloric mucosa is comminuted, extracted with water and the solids removed by centrifuging.  The solids are further extracted and to the combined extracts a solution of Vitamin B12 in distilled water is added, followed by a water solution of p-tert.-octyl-phenyl-diethoxy-dimethyl-benzyl ammonium chloride. To the clear solution, obtained by centrifuging, alcohol is added to precipitate the complex, which is separated by centrifuging and redissolved in water.  The so-formed solution is dialysed and the non-dialysable liquid lyophilised.ALSO:A therapeutic composition comprises a clear aqueous solution containing Vitamin C and a water-soluble non-dialysable Vitamin B12-hog stomach protein complex. The Vitamin B12-hog stomach protein complex may be prepared by adding a Vitamin B12 concentrate to an aqueous or saline extract of hog stomach, hog pyloric stomach or hog pyloric mucosa, precipitating the product from solution by addition of alcohol or other water-miscible organic solvent, dissolving the precipitate in water and dialysing. The non-dialysed solution may be used as it is, or lyophilized or it may be further purified by addition of a quaternary ammonium compound such as p-tert.-octyl-phenyl-diethoxy-dimethyl-benzyl ammonium chloride to precipitate contaminating water-soluble proteins, and removing the precipitate.  Other Vitamins, A, D, E, K, riboflavin, nicotinamide, thiamine, pantothenic acid and pyridoxine may also be added, where necessary solubilised by use of a polyoxyalkylene derivative of a sorbitan ester or a monoester of polyethylene glycol and an aliphatic carboxylic acid.  In all cases the term "vitamin" includes the vitamin per se and its salts and derivatives having the therapeutic efficacy of the vitamin. Preferably the final composition is prepared under nitrogen. Other additional ingredients include oil of orange, sucrose, glucose, saccharin and preservatives such as sodium benzoate.  Preparation of Vitamin B12-hog stomach protein complex.-In an example hog pyloric mucosa is comminuted, extracted with water and the solids removed by centrifuging.  The solids are further extracted and to the combined extracts a solution of Vitamin B12 in distilled water is added, followed by a water solution of p - tert. - octyl - phenyl - diethoxy - dimethyl - benzyl ammonium chloride.  To the clear solution obtained by centrifuging alcohol is added to precipitate the complex which is separated by centrifuging and redissolved in water.  The soformed solution is dialysed and the non-dialysable liquid is centrifuged and lyophilized. p  Preparation of the multivitamin composition.-An aqueous solution of Vitamin C is added to the solution of the Vitamin B12-non-dialysable hog stomach protein complex formed above. A further example describes a composition comprising Vitamin A palmitate, Vitamin D (irradiated ergosterol in corn syrup), oil of apple, a polyethylene glycol monostearate, Vitamin C (ascorbic acid), thiamine hydrochloride, pyridoxine hydrochloride, nicotinamide, sodium saccharin, monosodium riboflavin-51-phosphate, sodium pantothenate, sodium hydroxide and Vitamin B12-hog stomach protein complex.