Patent EA-004328-B1 (EA)
Title: Method for inducing viral resistance into a cell plant or a plant comprising a tgb3 sequence, and transgenic plant resistant to said virus
Inventor: GUILLEY HUBERT
IPC: A01H5/00
Filed: 1997-08-18
Abstract: 1. Method for inducing resistance to a virus comprising a TGB3 sequence with the proviso that it is not the potato virus X, comprising the following steps: - preparing a nucleic acid construct comprising a nucleic acid sequence TGB3 of said virus or its corresponding cDNA or variant thereof based on being operably linked to one or more regulatory sequence(s) active in a plant, - transforming a plant cell with the nucleic acid construct, and possibly - regenerating a transgenic plant from the transformed plant cell. 2. Method according to the claim 1, characterized in that the virus is selected from the group consisting of the apple stem pitting virus, the blueberry scorch virus, the potato virus M, the white clover mosaic virus, the orchid Cymbidium mosaic virus, the barley stripe mosaic virus, the potato mop top virus, the peanut clump virus and the beet soil-borne virus. 3. Method according to any of the preceding claims, characterized in that the plant cell is a stomatal cell. 4. Method according to any of the preceding claims, characterized in that the plant is selected from the group consisting of apple, blueberry, potato, clover, orchid, barley or peanut. 5. Method according to claim 1, characterized in that the virus is BNYVV, the nucleic acid sequence of TGB3 SEQ ID NO 1 given in Fig.6 comprised between the nucleotide 3627 and 4025 of the 5' strand of genomic or subgenomic RNA 2 of the BNYVV and the plant is a beet, preferably a sugar beet (Beta vulgaris). 6. Method according to any of the preceding claims, characterized in that the regulatory sequence comprises a promoter sequence or a terminator sequence active in a plant. 7. Method according to claim 6, characterized in that the promoter sequence is a constitutive or a foreigner vegetal promoter sequence. 8. Method according to the preceding claim 6, characterized in that the promoter sequence is selected from the group consisting of 35S Cauliflower Mosaic Virus promoter, and/or the polyubiquitin Arabidopsis thaliana promoter. 9. Method according to any of the claim 6 to 8, characterized in that the promoter sequence is a promoter which is mainly active in the root tissue of plants such as the par promoter of the haemoglobin gene from Perosponia andersonii. 10. Transgenic plant resistant to a virus obtained by the method according to claim 1. 11. Transgenic plant according to the claim 10, characterized in that the virus is selected from the group consisting of the apple stem pitting virus, the blueberry scorch virus, the potato virus M, the white clover mosaic virus, the orchid Cymbidium mosaic virus, the potato virus X, the barley stripe mosaic virus, the potato mop top virus, the peanut clump virus and the beet soil-borne virus. 12. Transgenic plant according to the claims 10 and 11 being a plant selected from the group consisting of apple, blueberry, potato, clover, orchid, barley or peanut. 13. Transgenic plant according to the claims 10, characterized in that the transgenic plant being a beet, preferably a sugar beet (Beta vulvaris), the virus is BNYVV with and the nucleic acid sequence of TGB3 SEQ ID NO 1 given in Fig.6 comprised between the nucleotide 3627 and 4025 of the 5' strand of genomic or subgenomic RNA 2 of the BNYVV. 14. Transgenic plant according to any of the preceding claims 10 to 13, characterized in that the regulatory sequence comprises a promoter sequence and a terminator sequence active in a plant. 15. Transgenic plant according to any of the preceding claims 10 to 14, characterized in that that the promoter sequence is a constitutive or a foreigner vegetal promoter sequence. 16. Transgenic plant according to the claim 15, characterized in that promoter sequence is selected from the group consisting of 35S Cauliflower Mosaic Virus promoter, and/or the polyubiquitin Arabidopsis thaliana promoter. 17. Transgenic plant according to claim 15 or 16, characterized in that the promoter sequence is a promoter which is mainly active in root tissues such as the par promoter of the haemoglobin gene from Perosponia andersonii.