Patent AU-2016204214-A1 (AU)
Title: Modulation of TIMP1 and TIMP2 expression
Inventor: NIITSU YOSHIRO
IPC: A61K48/00
Filed: 2016-06-21
Abstract: Provided herein are compositions, methods and kits for modulating expression of target genes, particularly of tissue inhibitor of metalloproteinase 1 and of tissue inhibitor of metalloproteinase 2 (TIMPi and TIMP2, respectively). The compositions, methods and kits may include nucleic acid molecules (for example, short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA) or short hairpin RNA (shRNA)) that modulate a gene encoding TIMPi and TIMP2, for example, the gene encoding human TIMPi and TIMP2. The composition and methods disclosed herein may also be used in treating conditions and disorders associated with TIMPi and TIMP2 including fibrotic diseases and disorders including liver fibrosis, pulmonary fibrosis, peritoneal fibrosis and kidney fibrosis. TIMP1 - TIMP metallopeptidase inhibitor 1 (SEQ ID NO:1) UUUCGUCGGCCCGCCCCUUGGCUUCUGCACUGAUGGUGGGUGGAUGAGUAAUGCAUCCAGGAAGCCU GGAGGCCUGUGGUUUCCGCACCCGCUGCCACCCCCGCCCCUAGCGUGGACAUUUAUCCUCUAGCGCU CAGGCCCUGCCGCCAUCGCCGCAGAUCCAGCGCCCAGAGAGACACCAGAGAACCCACCAUGGCCCCC UUUGAGCCCCUGGCUUCUGGCAUCCUGUUGUUGCUGUGGCUGAUAGCCCCCAGCAGGGCCUGCACCU GUGUCCCACCCCACCCACAGACGGCCUUCUGCAAUUCCGACCUCGUCAUCAGGGCCAAGUUCGUGGG GACACCAGAAGUCAACCAGACCACCUUAUACCAGCGUUAUGAGAUCAAGAUGACCAAGAUGUAUAAA GGGUUCCAAGCCUUAGGGGAUGCCGCUGACAUCCGGUUCGUCUACACCCCCGCCAUGGAGAGUGUCU GCGGAUACUUCCACAGGUCCCACAACCGCAGCGAGGAGUUUCUCAUUGCUGGAAAACUGCAGGAUGG ACUCUUGCACAUCACUACCUGCAGUUUUGUGGCUCCCUGGAACAGCCUGAGCUUAGCUCAGCGCCGG GGCUUCACCAAGACCUACACUGUUGGCUGUGAGGAAUGCACAGUGUUUCCCUGUUUAUCCAUCCCCU GCAAACUGCAGAGUGGCACUCAUUGCUUGUGGACGGACCAGCUCCUCCAAGGCUCUGAAAAGGGCUU CCAGUCCCGUCACCUUGCCUGCCUGCCUCGGGAGCCAGGGCUGUGCACCUGGCAGUCCCUGCGGUCC CAGAUAGCCUGAAUCCUGCCCGGAGUGGAAGCUGAAGCCUGCACAGUGUCCACCCUGUUCCCACUCC CAUCUUUCUUCCGGACAAUGAAAUAAAGAGUUACCACCCAGCAGAAAAAAAAAAAAAAAA TIMP2 - TIMP metallopeptidase inhibitor 2 (SEQ ID NO:2) CGCAGCAAACACAUCCGUAGAAGGCAGCGCGGCCGCCGAGAACCGCAGCGCCGCUCGCCCGCCGCCC CCCACCCCGCCGCCCCGCCCGGCGAAUUGCGCCCCGCGCCCCUCCCCUCGCGCCCCCGAGACAAAGA GGAGAGAAAGUUUGCGCGGCCGAGCGGGGCAGGUGAGGAGGGUGAGCCGCGCGGGAGGGGCCCGCCU CGGCCCCGGCUCAGCCCCCGCCCGCGCCCCCAGCCCGCCGCCGCGAGCAGCGCCCGGACCCCCCAGC GGCGGCCCCCGCCCGCCCAGCCCCCCGGCCCGCCAUGGGCGCCGCGGCCCGCACCCUGCGGCUGGCG CUCGGCCUCCUGCUGCUGGCGACGCUGCUUCGCCCGGCCGACGCCUGCAGCUGCUCCCCGGUGCACC CGCAACAGGCGUUUUGCAAUGCAGAUGUAGUGAUCAGGGCCAAAGCGGUCAGUGAGAAGGAAGUGGA CUCUGGAAACGACAUUUAUGGCAACCCUAUCAAGAGGAUCCAGUAUGAGAUCAAGCAGAUAAAGAUG UUCAAAGGGCCUGAGAAGGAUAUAGAGUUUAUCUACACGGCCCCCUCCUCGGCAGUGUGUGGGGUCU CGCUGGACGUUGGAGGAAAGAAGGAAUAUCUCAUUGCAGGAAAGGCCGAGGGGGACGGCAAGAUGCA CAUCACCCUCUGUGACUUCAUCGUGCCCUGGGACACCCUGAGCACCACCCAGAAGAAGAGCCUGAAC CACAGGUACCAGAUGGGCUGCGAGUGCAAGAUCACGCGCUGCCCCAUGAUCCCGUGCUACAUCUCCU CCCCGGACGAGUGCCUCUGGAUGGACUGGGUCACAGAGAAGAACAUCAACGGGCACCAGGCCAAGUU CUUCGCCUGCAUCAAGAGAAGUGACGGCUCCUGUGCGUGGUACCGCGGCGCGGCGCCCCCCAAGCAG GAGUUUCUCGACAUCGAGGACCCAUAAGCAGGCCUCCAACGCCCCUGUGGCCAACUGCAAAAAAAGC CUCCAAGGGUUUCGACUGGUCCAGCUCUGACAUCCCUUCCUGGAAACAGCAUGAAUAAAACACUCAU CCCAUGGGUCCAAAUUAAUAUGAUUCUGCUCCCCCCUUCUCCUUUUAGACAUGGUUGUGGGUCUGGA GGGAGACGUGGGUCCAAGGUCCUCAUCCCAUCCUCCCUCUGCCAGGCACUAUGUGUCUGGGGCUUCG AUCCUUGGGUGCAGGCAGGGCUGGGACACGCGGCUUCCCUCCCAGUCCCUGCCUUGGCACCGUCACA