Patent SG-11201906731X-A (SG)
Title: Media and methods for enhancing the survival and proliferation of stem cells
Inventor: HIRST ADAM
IPC: C12N5/0735
Filed: 2018-01-23
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 26 July 2018 (26.07.2018) WIPO I PCT omit VIII °nolo 0111 011101110010111 IIII 1111101 ow (10) International Publication Number WO 2018/132926 Al (51) International Patent Classification: C12N 5/071 (2010.01) C12N 5/0735 (2010.01) C12N 5/02 (2006.01) C12N 5/074 (2010.01) (21) International Application Number: PCT/CA2018/050076 (22) International Filing Date: 23 January 2018 (23.01.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/449,413 23 January 2017 (23.01.2017) US 62/518,776 13 June 2017 (13.06.2017) US 62/608,875 21 December 2017 (21.12.2017) US (71) Applicant: STEMCELL TECHNOLOGIES CANADA INC. [CA/CA]; 1618 Station Street, Suite 500, Vancouver, British Columbia V6A 1B6 (CA). (72) Inventors: HIRST, Adam; 1618 Station Street, Suite 500, Vancouver, British Columbia V6A 1B6 (CA). KARDEL, Melanie; 1618 Station Street, Suite 500, Vancouver, British Columbia V6A 1B6 (CA). CHANG, Wing; 1618 Station Street, Suite 500, Vancouver, British Columbia V6A 1B6 (CA). HIATT, Michael; 1618 Station Street, Suite 500, Vancouver, British Columbia V6A 1B6 (CA). JERVIS, Er- ic; 1618 Station Street, Suite 500, Vancouver, British Co- lumbia V6A 1B6 (CA). HUNTER, Arwen; 1618 Station Street, Suite 500, Vancouver, British Columbia V6A 1B6 (CA). (74) Agent: BERESKIN & PARR LLP/S.E.N.C.R.L., S.R.L.; Scotia Plaza, 40 King Street West, 40th Floor, Toronto, On- tario M5H 3Y2 (CA). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, — (54) Title: MEDIA AND METHODS FOR ENHANCING THE SURVIVAL AND PROLIFERATION OF STEM CELLS FIGURE 12 50 40 . 52 30 w w c)20 10 0 H1 cz lq. ( ..8 , . 0 ., .4, \ 6 3 , . 6 , .0 .0. .0 .. cs e v s , v s, „v.. ( 4\" • ks , N .ct • . E. oq tz1:: , c ,1 7. 0\" \ c x R . „ . x c p, 0 . G y x ~x x v.. c y. \Q'Z' yP ( vV. `(` cn‘ \" <0 q? Y ' 4 b 1-1 (57) : The present disclosure relates to improved supplements, culture media and methods for enhancing the survival or proliferation of mammalian stem cells. In particular, adding a lipid supplement, such as a lipid-enriched carrier (e.g. a lipid-enriched albumin), to the culture medium may enhance the survival and/or proliferation of the stem cells by at least 5% to 65% as compared to 0 a culture medium that does not contain the lipid supplement. C [Continued on next page] WO 2018/132926 Al MIDEDIMOMOIDEIREE30111001001010111110MEMNIE UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))