Patent EA-003857-B1 (EA)
Title: Peptide pharmaceutical composition and method to enhance elasticity of a tissue
Inventor: SANDBERG LAWRENCE B
IPC: A61K38/08
Filed: 1999-03-12
Abstract: 1. A composition, wherein said elastin peptide fragment is at a therapeutically effective concentration selected from the group consisting of Sequence No. 1 (Alanine-Valine-Glycine), Sequence No. 2 (Valine-Glycine-Alanine-Glycine), Sequence No. 3 (Isoleucine-Glycine-Glycine), Sequence No. 4 (Leucine-Glycine), Sequence No. 5 (Isoleucine-Glycine-Alanine-Glycine), Sequence No. 6 (Leucine-Glycine-Glycine), Sequence No. 7 (Valine-Alanine-Proline-Glycine), Sequence No. 8 (Leucine-Glycine-Proline-Glycine), Sequence No. 9 (Leucine-Glycine-Alanine-Glycine), Sequence No. 10 (Valine-Glycine-Proline-Glycine), Sequence No. 12 (Valine-Glycine-Proline-Glutamine), Sequence No. 13 (Leucine-Glycine-Alanine), Sequence No. 15 (Valine-Valine-Proline-Glycine), Sequence No. 16 (Alanine-Valine-Proline-Glycine), Sequence No. 17 (Valine-Valine-Proline-Glutamine), Sequence No. 18 (Valine-Alanine-Alanine-Arginine-Proline-Glycine), Sequence No. 19 (Leucine-Glycine-Alanine-Glycine-Glycine-Alanine-Glycine), Sequence No. 20 (Alanine-Isoleucine-Proline-Glycine), Sequence No. 21 (Leucine-Glycine- Proline-Glycine), Sequence No. 22 (Alanine-Alanine-Alanine-Glutamine-Alanine), Sequence No. 23 (Valine-Glycine-Valine-Hydroxyproline-Glycine), Sequence No. 24 (Valine-Tyrosine-Proline-Glycine-Glycine), Sequence No. 25 (Isoleucine-Glycine-Glycine-Valine-Glycine-Glycine), Sequence No. 26 (Valine-Alanine-Proline-Glycine-Valine-Glycine), Sequence No. 27 (Leucine-Glycine-Valine-Glycine-Glycine), Sequence No. 28 (Valine-Leucine-Proline-Glycine), Sequence No. 29 (Phenylalanine-Arginine-Alanine-Alanine-Alanine), Sequence No. 30 (Valine-Glycine-Glycine-Valine-Proline-Glycine), Sequence No. 31 (Phenylalanine-Glycine-Proline-Glycine-Glycine), Sequence No. 33 (Valine-Leucine-Proline-Glycine-Alanine-Glycine), Sequence No. 34 (Valine-Leucine-Proline-Glycine-Alanine-Glycine, Sequence No. 35 (Leucine-Glycine-Valine-Glycine-Alanine), Sequence No. 36 (Alanine-Phenylalanine-Proline-Glycine), Sequence No. 37 (AlaninePhenylalanine-Proline-Glycine-Alanine), Sequence No. 38 (Valine-Glycine-Isoleucine-Proline-Alanine), Sequence No. 39 (Valine-Glycine-Glycine-Isoleucine-Proline-Threonine), Sequence No. 40 (Valine-Glycine-Valine-Glycine-Valine-Proline-Glycine), Sequence No. 41 (Leucine-Glycine-Proline-Glycine-Valine-Glycine). 2. The method of claim 1, wherein the tissue in which elasticity is enhanced is skin. 3. The method of claim 1, wherein appearance of the skin is enhanced. 4. The method claim 1, further including the step of stimulating endogenous production of elastin. 5. The method of claim 1, wherein the tissue is a blood vessel. 6. The method of claim 1, wherein said tissue is deficient in elastin. 7. The method of claim 1, wherein said tissue is lung tissue. 8. The method of claim 1, wherein the step of administering the peptide is repeated over a predetermined time period. 9. The method of claim 1, wherein the predetermined time period exceeds 14 days of twice-daily administration of said peptide. 10. The method of claim 1, wherein said peptide is a hydrophilic elastin peptide. 11. The method of claim 1, wherein said peptide is formed by enzymatic cleavage of elastin with thermolysin. 12. The method of claim 1, wherein said peptide is included in a topical preparation to be applied to a patient's skin. 13. A pharmaceutical composition comprising a peptide, selected from the group consisting of Sequence No. 1 (Alanine-Valine-Glycine), Sequence No. 2 (Valine-Glycine-Alanine-Glycine), Sequence No. 3 (Isoleucine-Glycine-Glycine), Sequence No. 4 (Leucine-Glycine), Sequence No. 5 (Isoleucine-Glycine-Alanine-Glycine), Sequence No. 6 (Leucine-Glycine-Glycine), Sequence No. 7 (Valine-Alanine-Proline-Glycine), Sequence No. 8 (Leucine-Glycine-Proline-Glycine), Sequence No. 9 (Leucine-Glycine-Alanine-Glycine), Sequence No. 10 (Valine-Glycine-Proline-Glycine), Sequence No. 11 (Phenylalanine-Glycine-Proline-Glycine), Sequence No. 12 (Valine-Glycine-Proline-Glutamine), Sequence No. 13 (Leucine-Glycine-Alanine), Sequence No. 15 (Valine-Valine-Proline-Glycine), Sequence No. 16 (Alanine-Valine-Proline-Glycine), Sequence No. 17 (Valine-Valine-Proline-Glutamine), Sequence No. 18 (Valine-Alanine-Alanine-Arginine-Proline-Glycine), Sequence No. 19 (Leucine-Glycine-Alanine-Glycine-Glycine-Alanine-Glycine), Sequence No. 20 (Alanine-Isoleucine-Proline-Glycine), Sequence No. 21 (Leucine-Glycine-Proline-Glycine), Sequence No. 22 (Alanine-Alanine-Alanine-Glutamine-Alanine), Sequence No. 23 (Valine-Glycine-Valine-Hydroxyproline-Glycine), Sequence No. 24 (Valine-Tyrosine-Proline-Glycine-Glycine), Sequence No. 25 (Isoleucine-Glycine-Glycine-Valine-Glycine-Glycine), Sequence No. 26 (Valine-Alanine-Proline-Glycine-Valine-Glycine), Sequence No. 27 (Leucine-Glycine-Valine-Glycine-Glycine), Sequence No. 28 (Valine-Leucine-Proline-Glycine), Sequence No. 29 (Phenylalanine-Arginine-Alanine-Alanine-Alanine), Sequence No. 30 (Valine-Glycine-Glycine-Valine-Proline-Glycine), Sequence No. 31 (Phenylalanine-Glycine-Proline-Glycine-Glycine), Sequence No. 33 (Valine-Leucine-Proline-Glycine-Alanine-Glycine), Sequence No. 34 (Valine-Leucine-Proline-Glycine-Alanine-Glycine), Sequence No. 35 (Leucine-Glycine-Valine-Glycine-Alanine), Sequence No. 36 (Alanine-Phenylalanine-Proline-Glycine), Sequence No. 37 (Alanine-Phenylalanine-Proline-Glycine-Alanine), Sequence No. 38 (Valine-Glycine-Isoleucine-Proline-Alanine), Sequence No. 39 (Valine-Glycine-Glycine-Isoleucine-Proline-Threonine), Sequence No. 40 (Valine-Glycine-Valine-Glycine-Valine-Proline-Glycine), Sequence No. 41 (Leucine-Glycine-Proline-Glycine-V aline-Glycine). 14. The composition of claim 13, wherein said elastin peptide fragment is Sequence No. 17 (Valine-Valine-Proline-Glutamine). 15. The composition of claim 13, wherein said elastin peptide fragment is Sequence No. 19 (Leucine-Glycine-Alanine-Glycine-Glycine-Alanine-Glycine). 16. The pharmaceutical composition of claim 13, wherein said elastin peptide fragment is Sequence No. 23 (Valine-Glycine-Valine-Hydroxyproline-Glycine). 17. The pharmaceutical composition of claim 13, wherein said peptide is derived from elastin. 18. The pharmaceutical composition of claim 13, wherein application of said composition to a patient results in neovascularization. 19. The pharmaceutical composition of claim 13, wherein application of said composition to a patient results in angiogenesis. 20. The pharmaceutical composition of claim 13, wherein said composition is a cosmetic preparation.