Patent EP-2049680-B1 (EP)
Inventor: JOSTEN ANDRE
IPC: C12Q1/68
Filed: 2007-07-03
Granted: 2010-12-29
Abstract: The procedure for the authentication of articles provided with a marking containing a marking nucleic acid, comprises providing an analysis solution, which contains two analysis- and two reference nucleic acid strands in segment-wise manner, bringing the analysis and reference solutions in contact with the marking under conditions suitable for a hybridizing of the analysis nucleic acid strand with marking nucleic acid, observing first and second fluorescence signals emitted from the marking and detecting the authenticity of the article. The procedure for the authentication of articles provided with a marking containing a marking nucleic acid, comprises providing an analysis solution, which contains two analysis nucleic acid strands and two reference nucleic acid strands in segment-wise, bringing the analysis solution and reference solution in contact with the marking under conditions suitable for a hybridizing of one of the two analysis nucleic acid strands with marking nucleic acid, observing first and second fluorescence signals emitted from the marking and detecting the authenticity of the article, if the observed fluorescence to an expected first characteristic of the first fluorescence signal is not observable and if an expected second characteristic corresponds to the second fluorescence signal. One of the two analysis nucleic acid strands is complementary to the marking nucleic acid. A first fluorophor at the analysis nucleic acid strand and a first quencher in a distance quenching in a first fluorescence signal of the first fluorophor at the other analysis nucleic acid strand are bound. The reference nucleic acid strands are complementary to marking nucleic acid and/or to one of the two analysis nucleic acid strands. A second fluorophor at a reference nucleic acid strand and a second quencher in a distance quenching in a second fluorescence signal of the second fluorophor at the other reference nucleic acid strand are bound. The first fluorophor and the first quencher are bound in the area of an end of analysis nucleic acid. The second fluorophor and the second quencher are bound in the area of an end of reference nucleic acid. The detection is carried out within 60 seconds after bringing in contact. The contacting and observing steps are carried out with ambient temperature. The analysis and reference nucleic acid are present in a hairpin structure, with which the analysis nucleic acid has two branches complementary to each other. The marking is illuminated with light of a given wavelength range. The marking is applied on a light-reflecting document. The analysis solution is applied on only one analysis field of the marking containing the marking nucleic acid. The analysis solution is placed with a spike on the marking. The expected first characteristic is a given first minimum intensity in a first wavelength range and a given damping behavior of the first fluorescence signal and the expected second characteristic is a given second minimum intensity in a second wavelength range and a given damping behavior of the second fluorescence signal. The marking contains a further nucleic acid except the marking nucleic acid. The first- and second fluorophor are identical. The marking is applied with a printing process on the article to be marked or on self-adhesive label. A printing ink is used as marking containing the marking nucleic acid and/or further nucleic acid. Independent claims are included for: (1) a kit with a first liquid donating mechanism containing reference solution; and (2) liquid donating mechanism.