Patent EA-001039-B1 (EA)
Title: Method for integrating of exzogenous dna into genome of a plant cell and method for producing fertile transgenic plant integrated into the genome of the exzogenous dna
Inventor: HANSEN GENEVIEVE
IPC: A01H5/00
Filed: 1996-09-12
Abstract: 1. A method for integrating of a exzogenous DNA into the genome of a plant cell wherein comprising transforming said cell of said DNA with a DNA fragment consisting of the DNA of interest bounded by T-DNA border sequences; and with at least one chimeric DNA or RNA molecule capable of expressing in said plant cell one or more proteins mediating the integration of the exzogenous DNA, wherein these proteins are encoded by the virulence region of an Agrobacterium Ti or Ri plasmid so that said proteins are present in a plant cell in a sufficient amount after introducing the exzogenous DNA into said plant cell but before the integration of the exzogenous DNA into the genome of said plant cell. 2. The method of claim 1 wherein the exzogenous DNA and chimeric DNA or RNA are introduced simultaneously. 3. The method of claim 1 wherein one or more proteins mediating the integration of the exzogenous DNA  encode VirD1, VirD2, VirC1, VirC2 or VirE2. 4. The method of claim 3 wherein one or more proteins mediating the integration of the exzogenous DNA  encode VirD1 and VirD2, or a combination of VirD1, VirD2 and VirE2. 5. The method of claim 3 wherein one or more proteins mediating the integration of the exzogenous DNA  encode VirD2. 6. The method of claim 1 wherein the exzogenous DNA bounded by T-DNA border sequences is part of a plant viral vector. 7. The method of claim 1 wherein said DNA gene is part of a plant viral vector. 8. The method of claim 1 wherein the exzogenous DNA bounded by T-DNA border sequences and said DNA gene are part of a plant viral vector. 9. The method of claim 1herein said plant cell is a cell of a dicotyledonous plant. 10. The method of claim 9 wherein said dicotyledonous plant is selected from th e group consisting of tobacco, cotton, oilseed rape, and soybean. 11. The method of claim 1 wherein said plant cell is a cell of a monocotyledonous plant. 12. The method of claim 13 wherein said monocotyledonous plant is selected from the group consisting of maize, wheat and rice. 13. The method of claim 1 wherein said exzogenous DNA comprises a chimeric gene. 14. The method of claim 15 wherein said chimeric gene is capable of expressing a protein in said plant cell. 15. The method of claim 1 wherein said exzogenous DNA bounded by T-DNA border sequences and said DNA gene are components of a single DNA molecule. 16. A method for producing a fertile transgenic plant having DNA bounded by T-DN A border sequences integrated into the genome comprising transforming said DNA into the genome of a plant cell according to the method of claim 3; and regenerating the transformed plant cell to a fertile transgenic plant. 17. The method of claim 16 wherein said fertile transgenic plant is selected from the group consisting of tobacco, cotton, oilseed rape, soybean, maize, wheat and rice. 18. The method of claim 16 wherein said fertile transgenic plant is selected from the group consisting of maize, wheat, rye, sorghum, rice, barley, millet, sod-forming and forage grasses and also sugar cane sugar beet, oilseed rape, banana, poplar nut, tobacco and soybean.