Patent EA-002571-B1 (EA)
Title: Cruciferous sprouts with the rxception of brassica oleracea capitata, lepidium sativum, sinapis alba, sinapis nigra and raphanus sativus sprouts, method of their germination, a composition of glucosinolates and isothiocyanates from said sprouts, method for preparing thereof and human food product having chemoprotective and anti-cancerogenic action
Inventor: FAHEY JED W
IPC: A23L25/00
Filed: 1996-09-13
Abstract: 1. A method of obtaining cruciferous sprouts with the exception of Brassica oleracea capitata, Lepidium sativum, Sinapis alba, Sinapis nigra, and Raphanus sativus sprouts, comprising germinating cruciferous seeds and harvesting sprouts prior to the 2-leaf stage containing high phase 2 enzyme-inducing potential and non-toxic levels of indole glucosinolates and their breakdown products and goitrogenic hydroxybutenyl glucose isolates when measured after 3-days of growth. 2. The method according to claim 1, wherein said sprouts are harvested 1 to 14 days post-germination and contain at least 200,000 units per gram fresh weight of Phase 2 enzyme-inducing potential. 3. The method according to claim 1 or 2, wherein said seeds are a Brassica oleracea selected from the group of varieties consisting of acephala, alboglabra, botrytis, costata, gemmifera, gongylodes, italica, medullosa, palmifolia, ramosa, sabauda, sabellica, and selensia. 4. The method according to claim 3, wherein said seeds are Brassica oleracea variety italica, botrytis and botrytis subvariety cauliflora. 5. Cruciferous sprouts with the exception of Brassica oleracea capitata, Lepidium sativum, Sinapis alba, Sinapis nigra, and Raphanus sativus sprouts containing high phase 2 enzyme-inducing potential and non-toxic levels of indole glucosinolates and their breakdown products and goitrogenic hydroxybutenyl glucose isolates obtained according to any of claims 1 to 4. 6. A method for preparing a composition of glucosinolates and isothiocyanates according to claim 5, wherein extracting glucosinolates and isothiocyanates from cruciferous sprouts or from cruciferous seeds or from a combination thereof with a non-toxic solvent, removing the extracted sprouts, seeds or a combination thereof from the resultant extract, and recovering the extracted glucosinolates and isothiocyanates. 7. The method according to claim 6, wherein said composition of the extracted glucosinolates and isothiocyanates is further dried by removing the solvent. 8. The composition of glucosinolates and isothiocyanates from cruciferous sprouts prepared acording to claim 6. 9. A human food product used for increasing the chemoprotective amount of Phase 2 enzymes or reducing the level of carcinogens in a mammal, wherein said pill or tablet is administered to a mammal upon ingestion of said food product, comprising cruciferous sprouts according to claim 5 and/or the composition of the extracted glucosinolates and isothiocyanates according to claim 8. 10. The human food product according to claim 9, wherein further comprises myrosinase enzyme. 11. The human food product according to any of claims 9 or 10, wherein comprises sprouts harvested 1 to 14 days post-germination and contain at least 200,000 units per gram fresh weight of Phase 2 enzyme-inducing potential and non-toxic levels of indole glucosinolates and their breakdown products and goitrogenic hydroxybutenyl glucose isolates when measured after 3-days of growth from which said sprouts are germinated.