Patent SG-11201805115P-A (SG)
Title: Detection and quantification of target nucleic acid sequence of a microorganism
Inventor: TAN MIN-HAN
IPC: C12Q1/68
Filed: 2016-12-19
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization M1111101110101011111 HO 111110111010111111011011101100 111110 ill OEN 11 International Bureau .. .... ..Yejd ..... .....!;,,, (10) International Publication Number (43) International Publication Date WO 2017/105353 Al 22 June 2017(22.06.2017) WIPO I PCT (51) International Patent Classification: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, C12Q 1/70 (2006.01) C12Q 1/68 (2006.01) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (21) International Application Number: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, PCT/SG2016/050611 KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, (22) International Filing Date: MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, 19 December 2016 (19.12.2016) NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, (25) Filing Language: English TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, (26) Publication Language: English ZA, ZM, ZW. (30) Priority Data: (84) Designated States (unless otherwise indicated, for every 10201510448Q 18 December 2015 (18.12.2015) SG kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, Connexis, Singapore 138632 (SG). DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, (72) Inventors: TAN, MM-Han; c/o Institute of Bioengineer- ing and Nanotechnology, 31 Biopolis Way, The Nanos, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, #04-01, Singapore 138669 (SG). VO, Thu Hong Anh; c/o GW, KM, ML, MR, NE, SN, TD, TG). Institute of Bioengineering and Nanotechnology, 31 Bi- Declarations under Rule 4.17: opolis Way, The Nanos, #04-01, Singapore 138669 (SG). — of inventorship (Rule 4.17(iv)) (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; Published: P.O. Box 1531, Robinson Road Post Office, Singapore — international 21(3)) 903031 (SG). with search report (Art. — with sequence listing part of description (Rule 5.2(a)) (81) Designated States (unless otherwise indicated, for every kind AE, AG, AL, AM, of national protection available): 1-1 M ir) M ir) 0 1-1 (54) Title: DETECTION AND QUANTIFICATION OF TARGET NUCLEIC ACID SEQUENCE OF A MICROORGANISM I r ----- (57) : The present invention provides a method for detecting and/or quantifying the presence of a target nucleic acid se - I-1 c:::: ) quence of a microorganism in a sample obtained from a subject, including amplifying the target sequence in a CpG island of the nuc - N leic acid of the microorganism, irrespective of the methylation status of the CpG island. The invention is embodied by a method for 0 detecting and/or quantifying the presence of a target nucleic acid sequence of Epstein-Barr virus (EBV) by amplifying a target se- quence in the BamHI-W region of EBV in cell free DNAs (cfDNAs) obtained from a subject. The present invention also provides a kit to be used for the method of the invention.