Patent GB-700557-A (GB)
Title: Improvements in or relating to antifoaming agents and compositions containing the same
IPC: B01D19/04
Filed: 1951-02-23
Abstract: A substantially non-foaming aqueous nutrient medium for use in the propagation of antibiotic producing micro-organisms is produced by adding to the medium a sufficient amount, e.g. 0.001-3.0 per cent. by weight, of an anti-foaming agent to substantially inhibit foaming, the agent being the condensation product of 0.75-6.0 mols. of ethylene oxide with 1 mol. of abietic acid or a product containing the same such as rosin, rosin oil or tall oil.  Examples of substantially non-foaming nutrient media are: for moulds sucrose 10.00 grams, NH4 Cl 0.12 grams, K2HPO4 (or KH2PO4) 0.05 grams, CaCl2 0.01 grams, MgSO4 0.02 grams, condensation product of ethylene oxide with tall oil in molar ratio of 1:1 0.25 grams, water 100.00 grams; for penicillin lactose 2.500 per cent. by weight, Cerelose .5 per cent. by weight, corn steep liquor solids 2.0 per cent. by weight, ZnSO4 0.005 per cent. by weight, CaCO3 0.1 per cent. by weight, precursor 0.1 per cent. by weight, vegetative inoculum (penicillium notatum) 7.0 per cent. by weight, condensation product of ethylene oxide with tall oil in molar ratio of 3:1 3.0 per cent. by weight, water 84.815 per cent. by weight.  Examples of precursors which may be used are b -phenyl ethyl-amine acetate, N-(2, 2-diethoxyethyl) phenylacetamide, N-(1, 3-dihydroxy-2-propyl) phenyl-acetamide, phenethylamine, b -p-hydroxyphenethylamine, phenylaceto-nitrile, phenylacetamidine, b -phenyl-alamine, phenylpyruvic acid, phenyl acetamide, phenyl acetaldehyde, phenylacetic acid, and phenylacetyl-glycine; for streptomycin carbohydrate (dry weight) 2.0 per cent. by weight, soybean meal 2.0 per cent. by weight, corn steep liquor solids 0.5 per cent. by weight, NaCl 0.25 per cent. by weight, CaCO3 0.10 per cent. by weight, vegetative inoculum (Streptomyces griseus) 7.00 per cent. by weight, condensation product of ethylene oxide with tall oil in molar ratio of 1:1 3.00 per cent. by weight, water 85.95 per cent. by weight.ALSO:A foam inhibiting agent for use in aqueous nutrient media used in the propagation of yeast, moulds, related micro-organisms of fermentation, their derivatives and by-products (see Groups VI and IV (b)) is the condensation product of 0.75-6.0 mols of ethylene oxide with 1 mol of abietic acid or a product containing the same such as rosin, rosin oil or tall oil.ALSO:A substantially non-foaming aqueous nutrient medium for use in the propagation of yeast, moulds, related microorganisms of fermentation, their derivatives and by-products, is produced by adding to the medium a sufficient amount e.g. 0.001-3.0 per cent by weight, of an anti-foaming agent to substantially inhibit foaming, the agent being the condensation product of 0.75-6.0 mols of ethylene oxide with 1 mol of abietic acid or a product containing the same such as rosin, rosin oil or tall oil. Examples of substantially non-foaming nutrient media are: for yeasts and moulds, sucrose 10.00 grams, NH4C1 0.12 grams, K2HPO4 (or KH2PO4) 0.05 grams, CaC12 0.01 grams, MgSO4 0.02 grams, condensation product of ethylene oxide with tall oil in molar ratio of 1 : 1 0.25 grams, water 100.00 grams.