Patent NZ-701432-A (NZ)
Title: Improved method and kit for determining severity and progression of periodontal disease
Inventor: KORNMAN KENNETH S
IPC: G01N33/50
Filed: 2011-12-09
Abstract: Disclosed is a method for determining whether a patient is predisposed to having severe periodontal disease and/or is predisposed to having high risk of progression of periodontal disease, comprising the following steps: (i) selecting a patient not presenting a sign of severe periodontal disease; (ii) isolating a nucleic acid sample from a biological sample obtained from said patient; (iii) contacting the nucleic acid sample with one or more primers capable of amplifying one or more fragments of the IL1B locus, each fragment comprising at least one of single nucleotide polymorphism (SNP) IL1B (rs16944; C/T), IL1B (rs1143623; G/C), or IL1B (rs4848306; C/T); (iv) amplifying the one or more fragments of the IL1B locus such that each of SNP IL1B (rs16944; C/T), IL1B (rs1143623; G/C), and IL1B (rs4848306; C/T) is included in one or more amplified fragments; (v) detecting the number of copies of allele 1 and allele 2 of each of said SNPs IL1B (rs16944; C/T), IL1B (rs1143623; G/C), and IL1B (rs4848306; C/T) in the one or more amplified fragments of step (iv); and (vi) determining that said patient is predisposed to having severe periodontal disease and/or is predisposed to having high risk of progression of periodontal disease when said patient has a composite genotype selected from the group consisting of: a. one copy of IL1B (rs16944; C/T) allele 1 (C), one copy of IL1B (rs1143623; G/C) allele 1 (G); and two copies of IL1B (rs4848306; C/T) allele 1 (C); b. two copies of IL1B (rs16944; C/T) allele 2 (T), one copy of IL1B (rs1143623; G/C) allele 1 (G), and two copies of IL1B (rs4848306; C/T) allele 1 (C); c. two copies of IL1B (rs16944; C/T) allele 1 (C), two copies of IL1B (rs1143623; G/C) allele 1 (G) and two copies of IL1B (rs4848306; C/T) allele 1 (C); d. one copy of IL1B (rs16944; C/T) allele 1 (C), two copies of IL1B (rs1143623; G/C) allele 1 (G) and two copies of IL1B (rs4848306; C/T) allele 1 (C); and e. two copies of IL1B (rs16944; C/T) allele 2 (T), two copies of IL1B (rs1143623; G/C) allele 1 (G) and two copies of IL1B (rs4848306; C/T) allele 1 (C); wherein the one or more primers in step (iii) are further capable of amplifying one or more fragments of the IL1B locus comprising single-nucleotide polymorphism (SNP) IL1B (rs1143633; G/A) and wherein in step (iv), each of SNP IL1B (rs16944; C/T), IL1B (rs1143623; G/C), IL1B (rs4848306; C/T), and IL1B (rs1143633; G/A) is included in the one or more amplified fragments; or wherein in step (iii), the nucleic acid sample is contacted with an additional one or more primers capable of amplifying a fragment of the IL1B locus which comprises SNP IL1B (rs1143633; G/A) and wherein in step (iv) each of SNP IL1B (rs16944; C/T), IL1B (rs1143623; G/C), IL1B (rs4848306; C/T), and IL1B (rs1143633; G/A) is included in the one or more amplified fragments; the method further comprising detecting in step (v) the number of copies of allele 1 and allele 2 of SNP IL1B (rs1143633; G/A) in the one or more amplified fragments obtained in step (iv).