Patent SG-11201900930U-A (SG)
Title: Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides
Inventor: ZHAO LISHAN
IPC: C12N15/81
Filed: 2017-08-11
Abstract: OHReb D OH Reb A OH OH FIG. A H O 00 O C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 15 February 2018 (15.02.2018) WIP0 I PCT omit VIII °nolo III ll VIII VIII (10) International Publication Number WO 2018/031955 A3 (51) International Patent Classification: C12N 15/81 (2006.01) Cl 2N 9/10 (2006.01) A23L 2/60 (2006.01) Cl 2N 9/90 (2006.01) (21) International Application Number: PCT/US2017/046637 (22) International Filing Date: 11 August 2017 (11.08.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/374,408 12 August 2016 (12.08.2016) US (71) Applicant: AMYRIS, INC. [US/US]; 5885 Hollis Street, Suite 100, Emeryville, California 94608 (US). (72) Inventors: ZHAO, Lishan; c/o Amyris, Inc., 5885 Hollis Street, Suite 100, Emeryville, California 94608 (US). LI, Wenzong; c/o Amyris, Inc., 5885 Hollis Street, Emerville, California 94608 (US). WICHMANN, Gale; c/o Amyris, Inc., 5885 Hollis Street, Suite 100, Emeryville, Califor- nia 94608 (US). KHANKHOJE, Aditi; c/o Amyris, Inc., 5885 Hollis Street, Suite 100, Emeryville, California 94608 (US). GARCIA DE GONZALO, Chantal; c/o Amyris, Inc., 5885 Hollis Street, Suite 100, Emeryville, California 94608 (US). MAHATDEJKUL-MEADOWS, Tina; c/o Amyris, Inc., 5885 Hollis Street, Suite 100, Emeryville, Cal- ifornia 94608 (US). JACKSON, Shaina; c/o Amyris, Inc., 5885 Hollis Street, Suite 100, Emeryville, California 94608 (US). LEAVELL, Michael; c/o Amyris, Inc., 5885 Hol- lis Street, Suite 100, Emeryville, California 94608 (US). PLATT, Darren; c/o Amyris, Inc., 5885 Hollis Street, Suite 100, Emeryville, California 94608 (US). (54) Title: UDP-DEPENDENT GLYCOSYLTRANSFERASE FOR HIGH EFFICIENCY PRODUCTION OF REBAUDIOSIDES (57) : Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-gly- cosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1_like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M. [Continued on next page] WO 2018/031955 A3 MIDEDIMOMOIDEIREIHRMIOMMUMOMEfin (74) Agent: PATHAK, Rahul et al.; Squire Patton Boggs (US) LLP, 1801 Page Mill Road, Suite 110, Palo Alto, California 94304 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to the identity of the inventor (Rule 4.17(i)) as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(11)) as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a)) (88) Date of publication of the international search report: 15 March 2018 (15.03.2018)