Patent SG-11201808787R-A (SG)
Title: Spheroid tissue microarray and methods of manufacture
Inventor: PLUMMER SIMON MATTHEW
IPC: C12N5/00
Filed: 2016-12-09
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 1111111101111011101010111110101111101110101110111111111110111101111101111011111 International Bureau 0.. .... .. ..... ..or::,„, (10) International Publication Number (43) International Publication Date WO 2017/174955 Al 12 October 2017(12.10.2017) WIPO I PCT (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12N 5/00 (2006.01) Cl 2M 1/32 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (21) International Application Number: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, PCT/GB2016/053907 DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (22) International Filing Date: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, 9 December 2016 (09.12.2016) KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, (25) Filing Language: English NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (26) Publication Language: English RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, (30) Priority Data: ZA, ZM, ZW. 62/390,660 6 April 2016 (06.04.2016) US 1615517.8 13 September 2016 (13.09.2016) GB (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (71) Applicant: MICROMATRICES ASSOCIATES LTD GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, [GB/GB]; 23 Wellgate Street, Newport on Tay, Fife DD6 TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, 8HS (GB). TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, (72) Inventor: PLUMMER, Simon Matthew; 23 Wellgate DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, Street, Newport on Tay, Fife DD6 8HS (GB). LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, (74) Agent: HINDLE, Alistair Andrew; Alistair Hindle Asso- GW, KM, ML, MR, NE, SN, TD, TG). ciates Limited, 66 Hanover Street, Edinburgh EH2 1EL — Published: — (GB). — — — with international search report (Art. 21(3)) = = (54) Title: SPHEROID TISSUE MICROARRAY AND METHODS OF MANUFACTURE - = =\"%t7or 12 14 1 2 14 12 4 = = - = = FIG. 1A F bbaoflanl :arces(0 7%)over 12 FIG. 14 12 1B 14 12 14 - = Il (57) : A spheroid tissue 1 1 in in may be impregnated with a wax or t;;;N grid. A method of manufacturing a 'V mold material in a casting mold, and I N ping up the porous mold with further ing-in of liquid mold material through IN 1-1 surface of the porous mold; and sealing the tissue C the liquid mold material to set. An N material in a casting mold; allowing 0 oids in recesses at the bases of wells ,-t ther porous material on top of the .,. trusions of the casting mold carrying further, the liquid in microarray comprises resin spheroid tissue microarray alternative method spheroids; wherein ‘ the allowing liquid mold shrinkage as , and FIG. mold 4 N // the nipple 1C sectioned, mold material to set; removing of liquid 14 the porous spheroids -shaped, material, the ix y an array of tissue and contains spheroids comprises the mold material to set; and allowing recesses liquid mold material within the mold comprises the steps material; and sealing recesses at the bases protrusions. of: forming spheroids embedded steps of: forming by the porous mold 1 sets; the of the removing which topping IT to FIG. placing tissue spheroids r 7 , form a wells 1D are the off mold in porous mold from the casting mold; top - in , a with the precisely the from within P svc '' within a porous mold. The product located in a regular geometric mold of porous material from liquid surface of the mold by the draw- spheroids into the recesses in the liquid mold material and allowing of porous material from liquid mold the casting mold; placing spher - the porous mold by adding fur - porous material are formed by pro -