Patent SG-11201903605T-A (SG)
Title: Distribution of engineered-cysteine caps
Inventor: BHARGAVA SWAPNIL
IPC: A61K47/50
Filed: 2017-11-06
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1 MEM 0 11101 HOE HI 1 0 011111111111100111111111011110 MAMIE Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\"\"1 WO 2018/085769 Al 11 May 2018 (11.05.2018) WIPO I PCT (51) International Patent Classification: (US). LEITH, Matthew J.; 21823 30th Drive SE, Bothell, A61K 39/395 (2006.01) C07K 16/00 (2006.01) Washington 98021 (US). A61K 47/50 (2017.01) C07K 16/28 (2006.01) A61K 51/10 (2006.01) C12N 15/09 (2006.01) (74) Agent: KIM-SZPAJDA, Jane et al.; SEATTLE GENETICS, INC., 21823 30th Drive SE, Bothell, Washing- (21) International Application Number: ton 98021 (US). PCT/US2017/060182 (81) Designated States (unless otherwise indicated, for every (22) International Filing Date: kind of national protection available): AE, AG, AL, AM, 06 November 2017 (06.11.2017) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (25) Filing Language: English DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (26) Publication Language: English HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, (30) Priority Data: KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 62/418,572 07 November 2016 (07.11.2016) US MG, OM, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (71) Applicant: SEATTLE GENETICS, INC. [US/US]; SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, 21823 30th Drive SE, Bothell, Washington 98021 (US). TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (72) Inventors: BHARGAVA, Swapnil; 21823 30th Drive (84) Designated States (unless otherwise indicated, for every SE, Bothell, Washington 98021 (US). CHEN, Cheng-Wei kind of regional protection available): ARIPO (BW, GH, Aaron; 21823 30th Drive SE, Bothell, Washington 98021 GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (54) Title: DISTRIBUTION OF ENGINEERED-CYSTEINE CAPS = Cys & 4 mom vs. Consfftion Ptiaeade: III II IIII in Ili III 2 • 0 240 1.5 1 es ek- lIl am 1 0.8- ak- 04 - e2 - 0 ,,, — lima _111..__ _IN_ ....m.ii v `' 1-1 +` . 4 C:r V::+ IN kr) FIGURE 2 GC c: : :: , (57) : The present invention provides a method for removing cysteine caps from antibodies and re-capping the antibodies with c 3 - 1 -5 cysteine molecules. The methods include, inter alia, culturing a host cell comprising a protein molecule having at least one capped ,—I engineered cysteine residue, and contacting the cell culture with cystine. Dissolved oxygen levels can be manipulated in the cell culture 0 to ei further enhance the removal and re-capping process. C [Continued on next page] WO 2018/085769 Al MIDEDIMOMOIDEIREIDIONIMHIMMEHOMOVOIMIE TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(H)) — as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h))