Patent SG-11201910136R-A (SG)
Title: Methods for making high intensity sweeteners
Inventor: PATRON ANDREW
IPC: A23L27/20
Filed: 2018-05-02
Abstract: 1050_ YPD_ Mog5Opct_ 7mg_ ml_ Day2 5.0 1.91 _ 2 ' 13 2.43 MogV MogIllE 3.40 2 . 89 3.0 7 / 3.56 3.28 3.94 0.29 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00 4.20 4.40 4.60 4.80 5.00 MogIllE Compoundl- \ 2.85 1.31 1.26 1.97 0.0 - a 0 1250.000 - .T73 1000.000 - 750.000 - 0 - 1 500.000 - >. 250.000 - c.9 0.000 1.21 0.05 0.01 .82 1 91 . 2.05 2 54 . 2.12 2.61 3.43 2.96) 3.93 5.30 3 . 63 4.06 4 . 13 5.40 4.56 4 . 69 Range: 1760 *!IC cc 00 O O (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 08 November 2018 (08.11.2018) WIPO I PCT omit VIII °nolo III olo Imo mil mil onoilloo# (10) International Publication Number WO 2018/204483 A3 (51) International Patent Classification: C12P 33/12 (2006.01) A23L 27/20 (2016.01) C12N 15/52 (2006.01) (21) International Application Number: PCT/US2018/030627 (22) International Filing Date: 02 May 2018 (02.05.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/501,018 03 May 2017 (03.05.2017) US 62/551,750 29 August 2017 (29.08.2017) US (71) Applicant: SENOMYX, INC. [US/US]; 4767 Nexus Cen- tre Drive, San Diego, California 92121 (US). (72) Inventors: PATRON, Andrew, P.; 4767 Nexus Centre Drive, San Diego, California 92121 (US). NORIEGA, Chris, Edano; 4767 Nexus Centre Drive, San Diego, Cali- fornia 92121 (US). MANAM, Rama, R.; 4767 Nexus Cen- tre Drive, San Diego, California 92121 (US). COLQUITT, Justin; 4767 Nexus Centre Drive, San Diego, California 92121 (US). FABER, Nathan; 4473 Alabama Apt. 16, San Diego, California 92116 (US). ZIELER, Helge; 1155 Camino Del Mar #148, Del Mar, California 92014 (US). STEGE, Justin; 11355 Dennig Place, San Diego, Califor- nia 92126 (US). (74) Agent: LIU, Jing; KNOBBE, MARTENS, OLSON & BEAR, LLP, 2040 Main Street, 14th Floor, Irvine, Califor- nia 92614 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (54) Title: METHODS FOR MAKING HIGH INTENSITY SWEETENERS 3. Dode Airy Range: 6.684 0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 FIG. 37 (57) : Provided herein include methods of making mogroside compounds, e.g., Compound 1, compositions (for example host cells) for making the mogroside compounds, and the mogroside compounds made by the methods disclosed herein, and compositions (for example, cell lysates) and recombinant cells comprising the mogroside compounds (e.g., Compound 1). Also provided herein are novel cucurbitadienol synthases and the use thereof. [Continued on next page] WO 2018/204483 A3 MIDEDIMOMOIDEIRDERIHIMINHOIHNOMEMIE (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a)) (88) Date of publication of the international search report: 20 December 2018 (20.12.2018)