Patent SG-11201811781X-A (SG)
Title: High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications
Inventor: HALLOCK ALEXANDER
IPC: B03C1/005
Filed: 2017-06-27
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -,` 1#11011110111010101111101 11010101110111111011001111110111110 III OEN Organization International Bureau (10) International Publication Number (43) International Publication Date :::•••• WO 2018/005391 Al 04 January 2018 (04.01.2018) WIP0 I PCT (51) International Patent Classification: US 15/135,377 (CIP) BOIL 3/00 (2006.01) B03C 1/04 (2006.01) Filed on 21 April 2016 (21.04.2016) BO3C 1/005 (2006.01) BO3C 1/32 (2006.01) (71) Applicant: GENERAL AUTOMATION LAB TECH- BO3C 1/02 (2006.01) G01N 33/543 (2006.01) NOLOGIES, INC. [US/US]; 953 Indiana Street, Suite 372, (21) International Application Number: San Francisco, California 94107 (US). PCT/US2017/039346 (72) Inventors: HALLOCK, Alexander; 105 Camellia Av- (22) International Filing Date: enue, Redwood City, California 94061 (US). SHA, 27 June 2017 (27.06.2017) Michael; 7751 Coolidge Ct, Castro Valley, California 94552 (US). (25) Filing Language: English (74) Agent: CHEN, Yong; 358 5th Avenue, Suite 1003, New (26) Publication Language: English York, New York 10001 (US). (30) Priority Data: (81) Designated States (unless otherwise indicated, for every 62/357,142 30 June 2016 (30.06.2016) US kind of national protection available): AE, AG, AL, AM, (63) Related by continuation (CON) or continuation-in-part AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (CIP) to earlier application: CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (54) Title: HIGH RESOLUTION SYSTEMS, KITS, APPARATUS, AND METHODS USING MAGNETIC BEADS FOR HIGH THROUGHPUT MICROBIOLOGY APPLICATIONS 2230 2220 magnet 2220 , ‘%\" - \"pr;%/0 5 ,\"/K// - /.., 2220 ,, ,,,.../ • .,..; . / .ip „, 4 4 1-1, 7 4 7 7 „ArAe\" , p\" , , , W\",,,/,',/,e, /e5' .. f / . x 0, 4 / A Mi \" • ' 2223 2221 - . / ,,:. e..• • 2211 , , AZ /' , Z/.0,45A%\" . •.: ) ,',/!,;, , ,3/41/347.,„\"': ; 44 ;%.,, .We;;, -;;0107 /r5; 2210 2215 2210 2210 — Chips Further use of Beads alignment transfer duplicate chips FIG. 22 1-1 1-1 (57) : A method of transferring material from a first microfabricated device to a second microfabricated device. At least one ct. en magnetic bead is loaded into at least one microwell of the first microfabricated device, where a plurality of cells are cultivated. The III) second microfabricated device is positioned such that the at least one microwell of the first array of microwells is aligned with at least C one microwell of the second array of microwells. A magnetic field is applied so as to move the at least one magnetic bead contained --.... in the at least one microwell of the first microfabricated device into the at least one microwell of the second microfabricated device. In *1:) cell from the plurality of cells in the at least one microwell of the first microfabricated device is transferred 1-1 this manner, at least one © to the at least one microwell of the second microfabricated device. ei C [Continued on next page] WO 2018/005391 Al MIDEDIMOMOIDEIREEMOOVIREINIHOIIMEMOIMIE HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))