Patent DE-102008063592-A1 (DE)
Title: Sterile process bag for carrying out a multistage process in a closed system, comprises a carrier matrix with biomolecules to be cleaned in the bag, and an electrode pluggable channel and/or buffer bridges for electro-elution
Inventor: SCHINDELHAUER DIRK
IPC: C12Q1/00
Filed: 2008-12-18
Abstract: The sterile process bag comprises a carrier matrix with biomolecules to be cleaned in the bag and sterile connection under pure room condition through reversible closing, shutting, irreversible closing or sealing, an electrode pluggable channel and/or buffer bridges for the electro-elution of the cleaned biomolecules from the carrier matrix, and an electro-elution device and an elute chamber for infiltrating/filling the cleaned molecules in a transport tube and closed section of the elute chamber or the transport tube. The sterile process bag comprises a carrier matrix with biomolecules to be cleaned in the bag and sterile connection under pure room condition through reversible closing, shutting, irreversible closing or sealing, an electrode pluggable channel and/or buffer bridges for the electro-elution of the cleaned biomolecules from the carrier matrix, an electro-elution device and an elute chamber for infiltrating/filling the cleaned molecules in a transport tube and closed section of the elute chamber or the transport tube, an access for electrophoresis that guarantees escaping of electrophoresis gases and/or buffer flow, ports for a gas reduction opening jointly with buffer outlet, ports/outlet for the supply and discharge of incubation buffer, enzymes, electrophoresis buffer, buffer bridges and electrodes, inlet for electrophoresis buffer, outlet in which pressure-regulatable swelling of the bag is carried out through buffer volume and regulation of inlet/outlet-water column difference or swelling the bag through pump pressure or through stable support/walls, flow channel/port and tube and clasp for rapid emptying the incubation buffer, inlet access/accesses for the infection of pure buffer cascades and the inflow of cooled electrophoresis buffer/elution buffer, access for the separate application of enzymes and enzyme solution, alternatively with multifunctional access/ports, a drying chamber for sterile supply of nucleases after the steam sterilization of he bag, insertion channel for electrodes or electrode film alternatively integrated in to the process bag, outlet channel/hose for gas/electrophoresis buffer outflow, fixer, fixing groove, welding seam, form zones and clamp zones arranged internal or external of the bag for placing the carrier matrix in the process bag, hollow form, base with sides and clasp cover or separate cover, permeable hollow form/frame with support, frame with support, hollow form/frame with device for fixing/plugging electrodes and couplable electrode holders, frame for the reception of the portion of the carrier matrix, frame with a base film/sealing, sieve frame for the stabilization of the carrier matrix in simultaneous accessibility for buffer, enzymes, cleaning solutions, washing buffer, electrophoresis buffer, electro elution buffer, stream, permeable for macro molecules from solid plastic. The biomass is produced in the form of gel, colloid, gel matrix, agarose gel, low melting point gel, bacteria in gel matrix, Escherichia coliclone in low melting point agarose matrix with determined pore size, determined surface, silicate, ceramics, sieve, lamella, area, thread with antibody, binding molecules, receptor binding position, receptors, nucleic acid, DNA sequence, DNA binding protein, histones and materials. The process is carried out by supplying a biomass in the form of a carrier matrix, cleaning the organic molecules, protectively storing the organic molecules during the process step and obtaining samples in the closed system, identifying the functionality of the stored bio-molecules based on the sample, separating a portion of molecular layers in closed system, isolating and purely preparing the sterile stored biomolecules, and separating and filling the fraction in closed system. According to the method, biomolecules are pure forms of molecular species defined in the biological framework of the molecular conformity, where the molecular species comes from a material of a living system, or is isolated from an introduced biomass, animals, plants, human, microorganism masses and liquids, living or dead, including environmental samples, sediments, sludges, with the target of producing in high purity, where pure forms of a defined, small number of one or two, or a few individual similar in character of biomolecule species (e.g. genes, collagens) include the multiple biological variants of a biomolecule species (e.g. gene allele, copy number variants, splice variants, chain length variants, collagen forms etc.) so that it is the diversity of the molecules to be isolated (e.g. thousands of medically relevant gene segments, thousands of medically relevant gene products, proteins, enzymes, antibodies, factors and biomolecules), which are placed in the form of banks, which contain hundreds of thousands of potentially relevant biomolecules. The closed container system that comes as single article is used for sample reception, the storage and packaging of product. The wall of the bag is made of flexible, transparent, film and bag materials. Independent claims are included for: (1) a method for filling multiple chambers; (2) an electrode material for a process bag; (3) a method for subjecting electrode adaptors and cable at the process bag electrodes; (4) a method for carrying out a complex, versatile labor process with the process bag; and (5) a method for production, provision and marketing of penetration device.