Patent SG-11201806561U-A (SG)
Title: Method of isolating mesenchymal stromal cells and applications for tissue engineering
Inventor: LAWRENCE ANTHONY
IPC: G01N1/10
Filed: 2016-09-30
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property , Organization 1111111111101101111111111111111101011111H1101111111111100111111111110111111 International Bureau ...... .51 j ..... ....1 (10) International Publication Number (43) International Publication Date . WO 2017/058003 A2 6 April 2017 (06.04.2017) WIPO I PCT (51) (21) (22) (25) Filing Language: (26) Publication Language: (30) (71) Applicant: (72) International Patent Classification: Menara UOA Bangsar, 5 Jalan Bangsar Utama 1, Taman C12N 5/0775 (2010.01) Cl 2M 1/26 (2006.01) Bangsar, Kuala Lumpur, 59000 (MY). C12N 5/077 (2010.01) GO1N 1/10 (2006.01) C12Q 1/68 (2006.01) (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, International Application Number: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, PCT/MY2016/050063 BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, International Filing Date: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, 30 September 2016 (30.09.2016) KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, English MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, Priority Data: TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, PI 2015002493 2 October 2015 (02.10.2015) MY ZW. ELIXIR BIOPHARMA SDN. BHD. (84) Designated States (unless otherwise indicated, for every [MY/MY]; Unit 1110 Block A, Pusat Dagangan Phileo kind of regional protection available): ARIPO (BW, GH, Damansara II, 15 Jalan 16/11, Off Jalan Damansara, Petal- GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, ing Jaya, Selangor, 46350 (MY). TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, Inventor: LAWRENCE, Anthony; Unit 1110 Block A, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, Pusat Dagangan Phileo Damansara II, 15 Jalan 16/11, Off LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, Jalan Damansara, Petaling Jaya, Selangor, 46350 (MY). SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, Agents: HEMINGWAY, Christopher Paul et al.; Marks GW, KM, ML, MR, NE, SN, TD, TG). & Clerk (Malaysia) Sdn Bhd, Unit 6, Level 20, Tower A, [Continued on next page] = = (74) — = Title: METHOD OF ISOLATING MESENCHYMAL STROMAL CELLS AND APPLICATIONS FOR TISSUE ENGINEER- Figure 1 (54) ING (57) : A method of isolating mes- enchymal stromal cells from umbilical cord, foreskin, cornea or dental pulp whereby the action of collagenase is quenched such that the tissue sample is only partially digested. = = \\\\\ „ .. .. .... .... .... .... .... .... .... , .... , , , , , , \\ . \\NN WJSC \.:: 6 , i,:•,„ :# #: \ :# , ' \ .„\\ • \ .• ''','. .'i.‘ ...:‘ ' ' ' s 's X \ \"\" :•. . \ N::., s \ \\ \ \ \ \N. , \ N' • \ :, \ ',.., A, \ , ., • Fpcs s ,:\‘ ,\ s.. , \ ,:, 0 SHED :,, --a- ... = = ' 'X\ \ *. %.• ::,,‘ \ N % ‘,' *\ . NV . , . \\... . \ \ \ . \.\ \ \\.\\\ \\,;\\\\ % , , \::::, • %\. \\CLsc = el 0 kin IN ,-1 © ei O WO 2017/058003 A2 MIDIOH011111 1111NMEM1111111111111111111111111111111111111110111111111111 Declarations under Rule 4.17: Published: — of inventorship (Rule 4.17(iv)) — without international search report and to be upon receipt of that report (Rule 48.2(g)) — with sequence listing part of description (Rule republished 5.2(a))