Patent SG-11201906434T-A (SG)
Title: Method for analyzing and selecting a specific droplet among a plurality of droplets and associated apparatus
Inventor: REICHEN MARCEL
IPC: B01L3/00
Filed: 2018-01-18
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 26 July 2018 (26.07.2018) WIPO I PCT oimitia °nolo 0111 D oimIE (10) International Publication Number WO 2018/134323 Al (51) International Patent Classification: G01N 21/64 (2006.01) G01B 11/04 (2006.01) BOIL 3/00 (2006.01) G01N 15/14 (2006.01) G01J3/44 (2006.01) G01N 21/03 (2006.01) G01N 1/30 (2006.01) (21) International Application Number: PCT/EP2018/051245 (22) International Filing Date: 18 January 2018 (18.01.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 17305052.7 18 January 2017 (18.01.2017) EP (71) Applicant: HIFIBIO, PARIS [FR/FR]; 29, me du Faubourg Saint-Jacques, 75014 Paris (FR). (72) Inventors: REICHEN, Marcel; Engelstrasse 1, 8820 Wae- denswill (CH). DOINEAU, Raphael Clement Li-Ming; 4 rue Lacepede, 75005 Paris (FR). ELLOUZE, Sami; 23 rue des Tournelles, Appartment 9, 92290 Chatenay Malabry (FR). (74) Agent: CH KILGER ANWALTSPARTNERSCHAFT MBB; FasanenstraBe 29, 10719 Berlin (DE). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. — (54) Title: METHOD FOR ANALYZING AND SELECTING A SPECIFIC DROPLET AMONG A PLURALITY OF DROPLETS AND ASSOCIATED APPARATUS Figure 1 00 O O (57) : The present invention relates to a method for analyzing and selecting a specific droplet among a plurality of droplets (4), comprising the following steps: - providing a plurality of droplets (4), - for a droplet (4) among the plurality of droplets, measuring at least two optical signals, each optical signal being representative of a light intensity spatial distribution in the droplet for an associ- ated wavelength channel, - calculating a plurality of parameters from the optical signals, - determining a sorting class for a droplet according to calculated parameters, - sorting said droplet according to its sorting class, wherein the plurality of parameters comprises the coordinates of a maximum for each optical signal and a co-localization parameter and the at least two calculated parameters used for the determining step comprises the co-localization parameter. [Continued on next page] WO 2018/134323 Al MIDEDIMOMOIDEIREE30111111111001011100MEHOIS (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))