Patent SG-11201901822Q-A (SG)
Title: Single cell analysis of transposase accessible chromatin
Inventor: BELHOCINE KAMILA
IPC: C12Q1/68
Filed: 2018-05-25
Abstract: International Patent Classification: C12Q 1/68 (2018.01) (21) International Application Number: PCT/US2018/034774 (22) International Filing Date: 25 May 2018 (25.05.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: (71) Applicant: 10X GENOMICS, INC. [US/US]; 7068 Koll Center Parkway, Suite 401, Pleasanton, California 94566 (US). (72) Inventors: BELHOCINE, Kamila; 7068 Koll Center Parkway, Suite 401, Pleasanton, California 94566 (US). MCDERMOTT, Geoffrey; 7068 Koll Center Parkway, Suite 401, Pleasanton, California 94566 (US). MESCHI, Francesca; 7068 Koll Center Parkway, Suite 401, Pleasan- ton, California 94566 (US). ZHENG, Xinying; 7068 Koll Center Parkway, Suite 401, Pleasanton, California 94566 (US). 62/511,905 26 May 2017 (26.05.2017) US (74) Agent: COLE, Adam J.; WILSON SONSINI 62/527,529 30 June 2017 (30.06.2017) US GOODRICH & ROSATI, 650 Page Mill Road, Palo Alto, 15/842,550 14 December 2017 (14.12.2017) US US 94304 (US). 15/842,713 14 December 2017 (14.12.2017) US 15/842,687 14 December 2017 (14.12.2017) US (81) Designated States (unless otherwise indicated, for every 15/848,714 20 December 2017 (20.12.2017) US kind of national protection available): AE, AG, AL, AM, 62/650,223 29 March 2018 (29.03.2018) US AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, Irmelvdtir vt Flatbed weth r-adapteis ME BC Mr * BC.. Gel bead Ik FIG. 10 (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 29 November 2018 (29.11.2018) WIPO I PCT olimion ono o III OH onolom om iflo (10) International Publication Number WO 2018/218226 Al (54) Title: SINGLE CELL ANALYSIS OF TRANSPOSASE ACCESSIBLE CHROMATIN (57) : Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or co providing partitions (e.g., droplets or wells) including a single biological particle (e.g., a cell) and a single solid or semi-solid particle (e.g., bead) comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and 00 systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing O within the partitions. O [Continued on next page] WO 2018/218226 Al MIDEDIMOMOIDEIREEM00111MHOIROMEEN CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a))