Patent SG-11201906648R-A (SG)
Title: Method for normalizing a nucleic acid sample
Inventor: OSBORNE ROBERT
IPC: C12Q1/6806
Filed: 2018-01-10
Abstract: Reariimitiog amount of ow-specific endemic-maw () INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 26 July 2018 (26.07.2018) WIPO I PCT omit VIII °nolo 01110111 Ho iflo oimIE (10) International Publication Number WO 2018/134700 Al (51) International Patent Classification: C12Q 1/6806 (2018.01) (21) International Application Number: PCT/IB2018/050137 (22) International Filing Date: 10 January 2018 (10.01.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 1700941.6 19 January 2017 (19.01.2017) GB (71) Applicant: POPULATION GENETICS TECH- NOLOGIES LTD. [GB/GB]; Salisbury House, Station Road, Cambridge CB1 2LA (GB). (72) Inventor: OSBORNE, Robert; 38 Pilgrim Close, Great Chesterford, Saffron Walden CB10 1QG (GB). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) (54) Title: METHOD FOR NORMALIZING A NUCLEIC ACID SAMPLE (57) : Among other things, a method for normalizing a sample is provided. In some embodiments, the method comprises: (a) reacting a sample with a limiting amount of a single-turnover sequence-specific endonuclease that recognizes a target sequence, thereby cleaving a portion of the nucleic acid molecules that comprise the target se- quence and producing a normalized amount of a first cleavage product; and (b) isolat- ing, transcribing or selectively amplifying the normalized amount of the first cleavage product. In this method, because a limiting amount of the endonuclease is used, the nor- malized amount of the first cleavage product is determined by the limiting amount of the first single-turnover sequence-specific endonuclease used in step (a). W O 20 18/ 13 47 00 Al opiate deavageproducl Normalized Normalized Normalized Normalized sample E Pie 2 sample 3 I I I I I I I 1 Each normalized sample contains the same muter od molecules ESL* numaked sample contains exacny one 1 and rwo I molecules