Patent SG-11201908865W-A (SG)
Title: Apparatus and method for absolute quantification of biomarkers for solid tumor diagnosis
Inventor: ZHANG JIANDI
IPC: G01N33/53
Filed: 2018-06-28
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 03 January 2019 (03.01.2019) WIPO I PCT Iiiimmoimiolollmomioloololionmonilmom oimIE (10) International Publication Number WO 2019/006156 Al (22) International Filing Date: 28 June 2018 (28.06.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/526,425 29 June 2017 (29.06.2017) US (71) Applicant: QUANTICISION DIAGNOSTICS INC. [US/US]; 421 Granville Rd, Chapel Hill, North Carolina 27514 (US). (72) Inventor: ZHANG, Mandl; 6817 Homewood Dr., Chapel Hill, North Carolina 27514 (US). (74) Agent: XUE, Zhi Yang; Novick, Kim & Lee, PLLC, 3251 Old Lee Highway, Suite 404, Fairfax, Virginia 22030 (US). Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (51) International Patent Classification: GO1N 33/48 (2006.01) G01N 33/574 (2006.01) GO1N 33/53 (2006.01) GO1N 33/68 (2006.01) G01N 33/561 (2006.01) GOOF 19/24 (2011.01) (21) International Application Number: PCT/US2018/040075 (81) = (54) Title: APPARATUS AND METHOD FOR ABSOLUTE QUANTIFICATION OF BIOMARKERS FOR SOLID TUMOR DIAG- NOSIS FIG. 5 p<0.005 To co co 0 •E- 3 ? E 2 E 4- 40- 30- 20- p<0.0001 I p<0.0001 I I I ••• • • • T.) 1 0 aD • • ..„ ••• .• 6.: a) 0 II I III I Grade based on Nottingham Histologic score (57) : Disclosed here is a method for quantitative analysis of a sample. The method includes steps of (a) providing a singular marker representative of one or more features of the sample, the sample comprising a population of individual units of the marker; (b) measuring the marker with dot blot analysis, wherein the quantitation result is an absolute amount of the marker's population of individual units in the sample, normalized by the sample volume or by the sample weight; and (c) obtaining an objective determination of the one or more features of the sample based on the quantitation result of the marker. Also disclosed is a reference database and a method to use the reference database for diagnosing cancer in a patient. [Continued on next page] WO 2019/006156 Al O II UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))