Patent SG-11201408470X-A (SG)
Title: Methods and compositions for separating or enriching cells
Inventor: GUIA ANTONIO
IPC: G01N15/02
Filed: 2013-07-05
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 9 January 2014 (09.01.2014) WIPOIPCT (10) International Publication Number WO 2014/008487 A2 (51) International Patent Classification: BOIL 3/00 (2006.01) (21) International Application Number: PCT/US2013/049476 (22) International Filing Date: 5 July 2013 (05.07.2013) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 61/668,990 6 July 2012 (06.07.2012) US 13/844,085 15 March 2013 (15.03.2013) US (71) Applicant: AVIVA BIOSCIENCES CORPORATION [US/US]; 6330 Nancy Ridge Drive, Suite 103, San Diego, CA 92121-3220 (US). (72) Inventors: GUIA, Antonio; 6330 Nancy Ridge Drive, Suite 103, San Diego, CA 92121 (US). YAMANISHI, Douglas, T.; 6330 Nancy Ridge Drive, Suite 103, San Diego, CA 92121 (US). GHETTI, Andrea; 6330 Nancy Ridge Drive, Suite 103, San Diego, CA 92121 (US). TAO, Guoliang; 6330 Nancy Ridge Drive, Suite 103, San Diego, CA 92121 (US). TAO, Huimin; 6330 Nancy Ridge Drive, Suite 103, San Diego, CA 92121 (US). TRUONG, Ky; 6330 Nancy Ridge Drive, Suite 103, San Diego, CA 92121 (US). WU, Lei; 6330 Nancy Ridge Drive, Suite 103, San Diego, CA 92121 (US). WANG, Xiaobo; 6330 Nancy Ridge Drive, Suite 103, San Diego, CA 92121 (US). (74) Agents: CHEN, Peng et al.; Morrison & Foerster LLP, 12531 High Bluff Drive, Suite 100, San Diego, CA 92130- 2040 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. — [Continued on next page] (54) Title: METHODS AND COMPOSITIONS FOR SEPARATING OR ENRICHING CELLS FIGURE 33 SE Pumps: 3: Feed m 1: Buffer 2: Waste 00 •t oo o © ^ (57) Abstract: The present invention provides a filtration chamber comprising a microfabricated filter enclosed in a housing, ^ wherein the surface of said filter and/or the inner surface of said housing are modified by vapor deposition, sublimation, vapor-phase C^l surface reaction, or particle sputtering to produce a uniform coating; and method a for separating cells of a fluid sample, comprising: ^ a) dispensing a fluid sample into the filtration chamber disclosed herein; and b) providing fluid flow of the fluid sample through the filtration chamber, wherein components of the fluid sample flow through or are retained by the filter based on the size, shape, or de - formability of the components. WO 2014/008487 A2 llll II II11 III III II III III I I IIIII IIIII (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — without international search report and to be republished upon receipt of that report (Rule 48.2(g))