Patent AU-2013204582-B2 (AU)
Title: Humanized non-human animals with restricted immunoglobulin heavy chain loci
Inventor: MACDONALD LYNN
IPC: A01K67/027
Filed: 2013-03-07
Granted: 2016-01-14
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date W O 2013/187953 Al 19 December 2013 (19.12.2013) W I P 0 I P C T (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every A01K 67/027 (2006.01) C07K16/40 (2006.01) kind of national protection available): AE, AG, AL, AM, C07K16/28 (2006.01) C07K16/46 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, ) .a BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, (21) International Application Number: DO, DZ, EC, EE, EG, ES, Fl, GB, GD, GE, GH, GM, GT, PCT/US2013/029624 HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, (22) International Filing Date: KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, 7 March 2013 (07.03.2013) ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, (25) Filing Language: English RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, (26) Publication Language: English TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 61/658,466 12 June 2012 (12.06.2012) US (84) Designated States (unless otherwise indicated, for every 61/663,131 22 June 2012 (22.06.2012) US kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, (71) Applicant: REGENERON PHARMACEUTICALS, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, INC. [US/US]; 777 Old Saw Mill River Road, Tarrytown, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, NY 10591 (US). EE, ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (72) Inventors: MACDONALD, Lynn; 16 Gedney Way, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, White Plains, NY 10605 (US). MCWHIRTHER, John , OI (B, B, C, G,, 2152 Crescent Drive, Tarrytown, NY 10591 (US). TU, ML, MR, NE, SN, TD, TG). Naxin; 514 Washington Avenue, Pleasantville, NY 10570 Published: (US). STEVENS, Sean; 355 Berry Street #413, San Fran- with international search report (Art. 2](3) cisco, CA 94158 (US). MURPHY, Andrew, J.; 10 New ton Court, Croton-on-Hudson, NY 10520 (US). with sequence listing part of description (Rule 5.2(a)) (74) Agent: POBURSKY, Kevin J.; Choate, Hall & Stewart LLP, Two International Place, Boston, MA 02110 (US). (54) Title: HUMANIZED NON-HUMAN ANIMALS WITH RESTRICTED IMMUNOGLOBULIN HEAVY CHAIN LOCI (57) Abstract: Mice, embryos, cells, and tissues having a restricted immunoglobulin heavy chain locus and an ectopic sequence en coding one or more ADAM6 proteins are provided, in various embodiments, mice are described that have humanized endogenous f4 immunoglobulin heavy chain loci and are capable of expressing an ADAM6 protein or ortholog or homolog or functional fragment thereof that is functional in a male mouse. Mice, embryos, cells, and tissues having an immunoglobulin heavy chain locus character ized by a single human VH gene segment, a plurality of human DH gene segments and a plurality of human JH gene segments and cap able expressing an ADAM6 protein or ortholog or homolog or functional fragment thereof are also provided.