Patent SG-11201408107R-A (SG)
Title: Method for quantifying immune cells in tumoral tissues and its applications
Inventor: GALON JÉRÔME
IPC: G01N33/50
Filed: 2013-06-14
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 19 December 2013 (19.12.2013) WIPOIPCT (10) International Publication Number WO 2013/186374 A1 (51) International Patent Classification: G01N33/50 (2006.01) G01N33/574 (2006.01) (21) International Application Number: PCT/EP2013/062405 14 June 2013 (14.06.2013) (22) International Filing Date: (25) Filing Language: English (26) Publication Language: English (30) Priority Data: PCT/IB2012/001446 14 June 2012 (14.06.2012) IB (71) Applicants: INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE) [FR/FR]; 101, rue de Tolbiac, Paris, 75013 (FR). UNI- VERSITE PARIS DESCARTES [FR/FR]; 12, rue de l'Ecole de Medecine, F-75006 Paris 6 (FR). ASSIST­ ANCE PUBLIQUE HOPITAUX DE PARIS [FR/FR]; 3, avenue Victoria, F-75004 Paris (FR). (72) Inventors: GALON, Jerome; INSERM U872, Centre de Recherche des Cordeliers, 15 Rue de l'Ecole de Medecine, F-75006 Paris (FR). PAGES, Franck; INSERM U872, Centre de Recherche des Cordeliers, 15 Rue de l'Ecole de Medecine, F-75006 Paris (FR). MLECNIK, Bernhard; INSERM U872, Centre de Recherche des Cordeliers, 15 Rue de l'Ecole de Medecine, F-75006 Paris (FR). (74) Agents: PERIN, Georges et al.; Cabinet Plasseraud, 52 rue de la Victoire, F-75440 Paris Cedex 09 (FR). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) •rt l> m 00 i-H en i-H o CJ o & (54) Title: METHOD FOR QUANTIFYING IMMUNE CELLS IN TUMORAL TISSUES AND ITS APPLICATIONS (57) Abstract: A method for assessment of number a or density of immune cells in tumoral tissues comprising the steps consisting in: a. providing one or more immunostained slices of tissue section obtained by an automated slide-staining system by using antibod­ ies binding specifically to antigens (markers) expressed by immune cells, b. proceeding to digitalisation of the slides of step a. by high resolution scan capture, whereby a high definition (4.6 (xm/pixel or better) digital picture of the slide to be analysed is obtained, c. detecting the slice of tissue section on the digital picture d. analyzing the slice of tissue section for defining (i) the tumour (CT) and (ii) the invasive margin of the tumour (IM), e. providing a size reference grid with uniformly distributed units having a same sur­ face, said grid being adapted to the size of the tumour to be analyzed, el. checking the quality of immunostaining, detecting f. and quantifying stained cells of each unit whereby the number or the density of immune cells stained of each unit is assessed.