Patent SG-11201808183P-A (SG)
Title: Methods of heat inactivation of adenovirus
Inventor: MORRISON CHRISTOPHER
IPC: C12N7/04
Filed: 2017-03-28
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 11111111011110111010101111101011111011101011111010111011111011111101111011111 International Bureau .. .... ..Yejd ..... .....1 (10) International Publication Number (43) International Publication Date WO 2017/172772 Al 5 October 2017 (05.10.2017) IN I PO I P CT (51) International Patent Classification: (81) Designated States (unless otherwise C12N 15/861 (2006.01) C12N 7/04 (2006.01) kind of national protection available): C12N 15/864 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (21) International Application Number: DO, DZ, EC, EE, EG, ES, FI, GB, PCT/US2017/024545 HN, HR, HU, ID, IL, IN, IR, IS, (22) International Filing Date: KP, KR, KW, KZ, LA, LC, LK, 28 March 2017 (28.03.2017) MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, (25) Filing Language: English RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, (26) Publication Language: English TH, TJ, TM, TN, TR, TT, TZ, UA, ZA, ZM, ZW. (30) Priority Data: 62/314,116 28 March 2016 (28.03.2016) US (84) Designated States (unless otherwise kind of regional protection available): (71) Applicant: DIMENSION THERAPEUTICS [US/US]; GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, 840 Memorial Drive, 4th Floor, Cambridge, MA 02139 TZ, UG, ZM, ZW), Eurasian (AM, (US). TJ, TM), European (AL, AT, BE, (72) Inventors: MORRISON, Christopher, J.; 118 Lowell DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, Street, Arlington, MA 02474 (US). MARATT, James, D.; LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, 44 Lewis Road, Belmont, MA 02478 (US). SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, (74) Agents: DELFINO, Wyatt al.; Goodwin Procter LLP, et indicated, for every AE, AG, AL, AM, GD, GE, GH, GM, GT, JP, KE, KG, KH, KN, LR, LS, LU, LY, MA, PL, PT, QA, RO, RS, UG, US, UZ, VC, VN, indicated, for every ARIPO (BW, GH, AZ, BY, KG, KZ, RU, BG, CH, CY, CZ, DE, TG). 21(3)) Published: 100 Northern Avenue, Boston, MA 02210 (US). _ — — with international search report (Art. = = = = = Title: METHODS OF HEAT INACTIVATION OF ADENOVIRUS = (54) or particles, and a buffer. biological activity of The methods in - greater of divalent or = — FIG. 6 = 110% - - - - - - - - - - - - - - - -------- - ------- --- 7 - - - - - - - - - - - - = = - - - . ..__ - -,.... , .... = = .. .7 . ---- ,..„ ¨ Z - 1 -- ¨ _ - Background Buffer , 3.InsA Mg 12 Il ¨ ........ 10:riA MgC12 ¨ 5r4*1 MgC12 1 k MgC12 ei --4-- 100r0A1 Men - + -100nM MgC12 (Re aci) N O IN 50 19B 150 21q Exposure time (minutes) at 47•C 11 IN 1-1 (57) : The present disclosure generally relates to methods of protecting the genomic integrity and/or N AAV viral particles in a sample containing both AAV particles and helper virus particles during heat inactivation. dude heating, to a temperature greater than or equal to 45°C, a sample containing helper virus particles, AAV C The buffer includes a concentration of 10 mM or greater kosmotropic salts and/or a concentration of 10 mM trivalent cations.