Patent SG-11201908680Y-A (SG)
Title: Methods and compositions for preparing nucleic acid libraries
Inventor: Chen xi-jun
IPC: C12N15/10
Filed: 2018-03-16
Abstract: OHbp • - •1 3' 5' 5 P5 adaptor Phosphodiester linkage (S)— \\'‘` `'-J\"? zz/z//t/A OH 3 P7' 5' IP 3) 5'Phosphorylation PT Phosphodiester 64bp linkage Phosphodiester linkage 60bp 5' 4) CircLigase Ligation 5' OH ssDNA Insert 5' 3' OH: OH OH 3' G 5 P 1) 5'Dephosphonflation dsDNA Insert & Dehybridization 64bp 5' P ffeloW mw 3' 5' PT adaptor 2) CircLigase Ligation T4 Polynucleotide Phosphodiester Kinase linkage PT P5 (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 27 September 2018 (27.09.2018) WIP0 I PCT 011101 V III o Ho VII IE (10) International Publication Number WO 2018/175258 Al (51) International Patent Classification: Cl 2N 15/10 (2006.01) (21) International Application Number: PCT/US2018/022978 (22) International Filing Date: 16 March 2018 (16.03.2018) English English (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (25) Filing Language: (26) Publication Language: (30) Priority Data: 62/473,595 20 March 2017 (20.03.2017) US (84) (71) Applicant: ILLUMINA, INC. [US/US]; 5200 Illumina Way, San Diego, CA 92122 (US). (72) Inventors: CHEN, Xi-Jun; C/o Illumina, Inc., 5200 Il- lumina Way, San Diego, CA 92122 (US). KHURANA, Tarun; C/o Illumina, Inc., 5200 Illumina Way, San Diego, CA 92122 (US). (74) Agent: FULLER, Michael, L.; Knobbe Martens Olson & Bear LLP, 2040 Main Street, Fourteenth Floor, Irvine, CA 92614 (US). (54) Title: METHODS AND COMPOSITIONS FOR PREPARING NUCLELIC ACID LIBRARIES GC 5'-P5-ssDNA insert-P7 3' N FIG. 1 1-1 © (57) : Systems, methods and compositions provided herein relate to the preparation of nucleic acid libraries. Some embodi- O ments include the preparation of nucleic acid libraries by ligation of single-stranded nucleic acids. [Continued on next page] WO 2018/175258 Al MIDEDIM011111 1111E111 IIE3010M11111111101111111111111111111 Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a))