Patent SG-11201908384X-A (SG)
Title: Methods and compositions for inducible extracellular membrane capture of monoclonal immunoglobulins secreted by hybridomas
Inventor: DESSAIN SCOTT
IPC: C12N15/08
Filed: 2018-03-23
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 27 September 2018 (27.09.2018) D IV o Ho inciummo iflo VII IE (10) International Publication Number WO 2018/175917 Al WIPO I PCT (51) International Patent Classification: C12N 15/02 (2006.01) C12N 15/09 (2006.01) C12N 15/08 (2006.01) (21) International Application Number: PCT/US2018/024073 (22) International Filing Date: 23 March 2018 (23.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/476,599 24 March 2017 (24.03.2017) US 62/526,608 29 June 2017 (29.06.2017) US (71) Applicant: LANKENAU INSTITUTE FOR MEDICAL RESEARCH [US/US]; 100 Lancaster Avenue, Wyn- newood, PA 19096 (US). (72) Inventors: DESSAIN, Scott, K.; 1370 Indian Creek Drive, Wynnewood, PA 19096 (US). PULIGEDDA, Rama, De- vudu; 941 South Avenue, Apt C2, Secane, PA 19018 (US). (74) Agent: BAK, Mary, E. et al.; Howson & Howson LLP, 350 Sentry Parkway, Building 620, Suite 210, Blue Bell, PA 19422 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a)) (54) Title: METHODS AND COMPOSITIONS FOR INDUCIBLE EXTRACELLULAR MEMBRANE CAPTURE OF MONO- CLONAL IMMUNOGLOBULINS SECRETED BY HYBRIDOMAS N (57) : Compositions and methods are provided for making and using a cell having expressed on its outer plasma membrane surface an Anchor designed to form a first complex with a selected Linker. The Linker is designed to form a second complex with a target protein that is not recognized by the Anchor. These cells can be primary cells or immortalized cells, they function as fusion N partners to create hybridoma cells. The cells can be designed to bind a secreted targeted protein, e.g., a secreted antibody, to the cell surface via an immune complex formed by the Anchor-Linker and permit the identification of the existence, antigen specificity, and 00 antigen binding affinity, titer, amount, or biological activity of the target protein. In certain embodiments, the Linker is of a species O heterologous to the species of the Anchor; and the target protein is of a species heterologous to the Linker and to the Anchor. O