Patent SG-11201407595U-A (SG)
Title: Generation of human ips cells by a synthetic self- replicative rna
Inventor: DOWDY STEVEN F
IPC: C12N15/85
Filed: 2013-05-21
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 28 November 2013 (28.11.2013) WIPOIPCT (10) International Publication Number WO 2013/177133 A2 (51) International Patent Classification: Not classified (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/US2013/041980 21 May 2013 (21.05.2013) English (30) Priority Data: 61/649,876 61/798,229 21 May 2012 (21.05.2012) 15 March 2013 (15.03.2013) English US US (84) (71) Applicant: THE REGENTS OF THE UNIVERISITY OF CALIFORNIA [US/US]; 1111 Franklin Street, 5th Floor, Oakland, California 94607-5200 (US). (72) Inventors: DOWDY, Steven, F.; 5761 Waverly Avenue, La Jolla, CA 92037 (US). YOSHIOKA, Naohisa; 7665 Palmilla Drive, #5420, San Diego, CA 92122 (US). (74) Agent: BAKER, JR., Joseph, R.; Gavrilovich, Dodd & Lindsey LLP, 4660 La Jolla Village Drive, Suite 750, San Diego, CA 92122 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Published: — without international search report and to be republished upon receipt of that report (Rule 48.2(g)) — with sequence listing part of description (Rule 5.2(a)) (54) Title: GENERATION OF HUMAN IPS CELLS BY A SYNTHETIC SELF- REPLICATIVE RNA A) CJ m m i-H l> l> Non-Structural Proteins Structural Proteins orTransgenes VEE -| nsP1 | nsP2 | nsP3 | nsP4 Ml C I E2 E1 | H (-11,700 nt) VEE-GFP -| nsP1 I nsP2 | nsP3 | nsP4~HHPIilll' sl (-9,650 nt) VEE-OS H nsP1 I nsP2 I nsP3 nsP4 I MlQct4 jSoxZflillN' (~11,000nt) VEE-OMKS ^ nsP1 | nsP2 I risP3 | nsP4 Hi|Oct4 § cMyc | Klf4 jSox2| VEE-OKS-iM H nsP1 I nsP2 I nsP3 nsP4 I Mioct4lKlf4 i I cMyc VEE-OKS-iG -I nsP1 I nsP2 I nsP3 nsP4 I H;lOct4 i Klf4 §Sox2§ Ollstt |26S Pr 12A-peptide glRES Puro r EB 3'UTR & PolyA =PCR (-13,900 nt) (-14,500 nl) (-15,000 nt) i-H (57) Abstract: The disclosure provides methods and compositions useful for obtaining induced stem cells, methods of making and ® use thereof. CJ o &