Patent SG-11201806400X-A (SG)
Title: Replicative transposon system
Inventor: BUERCKSTUEMMER TILMANN
IPC: C12N15/90
Filed: 2017-02-10
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 17 August 2017 (17.08.2017) WIPO I PCT (10) International Publication Number WO 2017/137768 Al 11111111111111 0 111011111111111 010 11111 01110111 01111110 11111E1110 11111111111 0111111 (51) International Patent Classification: C12N 15/90 (2006.01) C12N 9/22 (2006.01) C12N 9/90 (2006.01) A61K 48/00 (2006.01) (21) International Application Number: PCT/GB2017/050355 (22) International Filing Date: 10 February 2017 (10.02.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 1602473.9 11 February 2016 (11.02.2016) GB (71) Applicants: HORIZON DISCOVERY LIMITED [GB/GB]; Building 7100, Cambridge Research Park, Wa- terbeach, Cambridge CB25 9TL (GB). MAX-DEL- BRUCK-CENTRUM FUR MOLEKULARE MEDIZIN [DE/DE]; Robert-Rossle-Str. 10, 13092 Berlin (DE). GE- NETIC INFORMATION RESEARCH INSTITUTE [US/US]; 465 Fairchild Drive, Suite 201, Mountain View, California 94043 (US). (72) Inventors: BUERCKSTUEMMER, Tilmann; c/o Hori- zon Discovery Limited, Building 7100, Cambridge Re- search Park, Waterbeach, Cambridge Cambridgeshire CB25 9TL (GB). KAPITONOV, Vladimir Vyachesla- vovich; c/o Genetic Information Research Institute, 465 Fairchild Drive, Suite 201, Mountain View, California 94043 (US). GRABUNDZIJA, Ivana; c/o Max-Delbrfick- Centrum fiir Molekulare Medizin, Robert-Rossle-Str. 10, 13092 Berlin (DE). IVICS, Zoltan; c/o Max-Del- brfick-Centrum ffir Molekulare Medizin, Robert-Rossle- Str. 10, 13092 Berlin (DE). (74) Agent: STRATAGEM IPM LIMITED; Meridian Court, Comberton Road, Tat, Cambridge Cambridgeshire CB23 2RY (GB). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) W O 20 17 / 1377 68 Al (54) Title: REPLICATWE TRANSPOSON SYSTEM (57) : The invention relates to a system and method for introducing DNA into cells. In particular, the invention relates to a method for introducing single or multiple copies of a DNA sequence or gene of interest into a cell comprising providing: a) a \"copy and paste\" transposase; and b) a construct comprising a DNA sequence or gene of interest flanked by a \"copy and paste\" transposon terminal sequence, such as an LTS or RTS. A novel \"copy and paste\" transposon of the Helitron family is described along with sys - tems for using the corresponding transposase in methods for introducing DNA into cells, for example, to generate cell lines for use in protein production, cell and gene therapy or as reference standards.