Patent SG-11201807478Q-A (SG)
Title: Methods and compositions for increased double stranded rna production
Inventor: KILLMER JOHN
IPC: C12N7/02
Filed: 2017-03-09
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 11 M11111011101010111110101111 101110111100110 110010 110 MINI III International Bureau .. .... ..Yejd (10) International Publication Number (43) International Publication Date .... ...!::,,,' WO 2017/160600 Al 21 September 2017 (21.09.2017) WIP0 I PCT (51) International Patent Classification: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, A01H 3/00 (2006.01) A01N 65/00 (2009.01) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, A01N 63/00 (2006.01) CO7H 21/02 (2006.01) DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, AO1N 63/02 (2006.01) Cl 2N 7/02 (2006.01) HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, (21) International Application Number: MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, PCT/US2017/021661 NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (22) International Filing Date: RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, 9 March 2017 (09.03.2017) TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (25) Filing Language: English (84) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of regional protection available): ARIPO (BW, GH, (30) Priority Data: GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, 62/308,381 15 March 2016 (15.03.2016) US TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, (71) Applicant: APSE, INC [US/US]; 4320 Forest Park Aven- DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, ue, Suite 303, St. Louis, MO 63108 (US). LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, (72) Inventors: KILLMER, John, L.; 4320 Forest Park Aven- SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). ue, Suite 303, St. Louis, MO 63108 (US). MCLAUGH- LIN, Patrick D.; 4320 Forest Park Avenue, Suite 303, St. Declarations under Rule 4.17: Louis, MO 63108 (US). ARHANCET, Juan Pedro Hum- - as to applicant's entitlement to apply for and be a granted berto; 4320 Forest Park Avenue, Suite 303, St. Louis, MO patent (Rule 4.17(ii)) 63108 (US). — of inventorship (Rule 4.17(iv)) Agents: CAMPBELL, John W. et al.; Morgan, Lewis & (74) Bockius LLP, Attn: Docketing Department, 1111 Published: Pennsylvania Avenue, NM., Washington, DC 20004 (US). with international search report (Art. 21(3)) Designated States (unless otherwise indicated, for every — with sequence listing part of description (Rule 5.2(a)) kind of national protection available): AE, AG, AL, AM, (81) Title: METHODS AND COMPOSITIONS FOR INCREASED DOUBLE STRANDED RNA PRODUCTION (54) Figure 1 1-1 © © ,tD , 0 tD 1-1 5 J\/3 , IN 1-1 (57) : Methods and materials for improved in vivo production of dsRNA are presented. Yields of dsRNA are significantly 0 increased in the presence of capsid protein. The improved yield of dsRNA is not dependent on the presence of specific cognate bind- \" ing sites for capsid protein associated with the dsRNA, but is dependent on capsid protein. C