Patent SG-11201901059Q-A (SG)
Title: Methods for purifying antibodies
Inventor: DUMETZ ANDRE C
IPC: C07K1/22
Filed: 2017-09-06
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 111111111111111111111111111111111111111111111111111111111111111111111111110111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\" WO 2018/047080 Al 15 March 2018 (15.03.2018) WI P0 I P C T (51) International Patent Classification: CO7K 1/22 (2006.01) Declarations under Rule 4.17: — as to applicant's entitlement to apply for and be granted a (21) International Application Number: patent (Rule 4.17(ii)) PCT/IB2017/055374 — as to the applicant's entitlement to claim the priority of the (22) International Filing Date: earlier application (Rule 4.17(iii)) 06 September 2017 (06.09.2017) Published: (25) Filing Language: Eng li sh — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the (26) Publication Language: English claims and to be republished in the event of receipt of (30) Priority Data: amendments (Rule 48.2(h)) 62/384,240 07 September 2016 (07.09.2016) US (71) Applicant: GLAXOSMITHKLINE INTELLECTUAL PROPERTY DEVELOPMENT LIMITED [GB/GB]; 980 Great West Road, Brentford Middlesex TW89GS (GB). (72) Inventors: DUMETZ, Andre C.; 709 Swedeland Road, King of Prussia, Pennsylvania 19406 (US). GOKLEN, Kent E.; 709 Swedeland Road, King of Prussia, Pennsylva- _ nia 19406 (US). LEVY, Nicholas E.; 709 Swedeland Road, King of Prussia, Pennsylvania 19406 (US). MOLEK, Jessi- - ca Rachel; 709 Swedeland Road, King of Prussia, Pennsyl- vania 19406 (US). THOMSON, Andrew S.; 709 Swede- = _ land Road, King of Prussia, Pennsylvania 19406 (US). = YANCEY, Kenneth G.; 224 South Geneva St., Apt. 1, Itha- ca, New York 14850 (US). _ (81) Designated States (unless otherwise indicated, for every _ kind of national protection available): AE, AG, AL, AM, = AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, = _ = MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, = — = OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. = Designated States (unless otherwise indicated, for every (84) = kind of regional protection available): ARIPO (BW, GH, = — GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, = UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). = _ 1-1 © GC © Ir ... ' 7r (54) Title: METHODS FOR PURIFYING ANTIBODIES 0 ---- (57) : The present invention relates to a method of purifying a recombinant polypeptide from Host Cell Proteins (HCP), the GC method comprising: (a) applying a solution comprising the recombinant polypeptide and HCP to a superantigen chromatography solid 0 support, (b) washing the superantigen chromatography solid support with a wash buffer comprising caprylate and arginine; and (c) N eluting the recombinant polypeptide from the superantigen chromatography solid support. C