Patent GB-362023-A (GB)
Title: Process for the production of extracts serving for therapeutic purposes and for increasing the vitamin content of foodstuffs
IPC: A61K31/16
Filed: 1930-09-11
Abstract: Vitamin concentrates suitable for therapeutic purposes or for addition to foodstuffs are obtained by extracting rootlets of grain or maize, germinated in malting, with ethyl alcohol or fat solvents, wholly freeing the extracts from the solvents, treating the residues with saponifying agents, precipitating the soaps, concentrating the filtrates and treating the residues with fat solvents, separating substances rich in vitamins from the solutions, for example, by freezing, and if desired dividing the product into fractions by means of petroleum spirit, or other fat solvent.  The manipulations following the precipitation of the soaps should be performed in an atmosphere free from oxygen, for example in a current or atmosphere of an inert gas such as nitrogen.  Fat solvents mentioned are acetone, ether, petroleum spirit, carbon disulphide, benzene, chloroform, carbon tetrachloride, trichlorethylene, liquid carbon dioxide, amyl alcohol, or mixtures thereof.  According to an example, air-dried rootlets are extracted with a mixture of acetone and petroleum spirit.  The extract is concentrated under reduced pressure to the consistency of a syrup, mixed with hot alcohol, and, if desired after filtration from the undissolved portion, treated with alcoholic soda or potash, or sodium alcoholate.  The soaps formed are precipitated with alcoholic calcium chloride solution and removed by filtration.  The solution is freed from alcohol by evaporation under reduced pressure, the residue extracted with warm acetone, the acetone evaporated, the residue redissolved in acetone, and the solution cooled to 20 DEG  C. in an atmosphere of dry nitrogen saturated with acetone vapour.  The precipitated mass is washed with acetone and then extracted with warm petroleum spirit. The extract thus obtained is frozen at 20 DEG  C., and the crystals obtained aspirated in nitrogen and washed in acetone at 20 DEG  C. These crystals and finally the residue left on evaporating the petroleum spirit mother liquor are dried in vacuo, dissolved in ethyl alcohol freed from air by means of nitrogen, and the solutions kept in hermetically sealed bottles.  Specification 328,942, [Class 2 (iii), Dyes &c.], is referred to.  The Specification as open to inspection under Sect. 91 (3) (a) comprises also another example, in which the insoluble soap is washed with ether, and the washings added to the alcoholic solution separated from the soap.  This subject-matter does not appear in the Specification as accepted.ALSO:Vitamin concentrates suitable for therapeutic purposes or for addition to foodstuffs are obtained by extracting rootlets of grain or maize, germinated in malting, with ethyl alcohol or fat solvents, wholly freeing the extracts from the solvents, treating the residues with saponifying agents, precipitating the soaps, concentrating the filtrates and treating the residues with fat solvents, separating substances rich in vitamins from the solutions, for example by freezing, and if desired dividing the product into fractions by means of petroleum spirit, or other fat solvent.  The manipulations following the precipitation of the soaps should be performed in an atmosphere free from oxygen, for example in a current or atmosphere of an inert gas such as nitrogen.  Fat solvents mentioned are acetone, ether, petroleum spirit, carbon disulphide, benzene, chloroform, carbon tetrachloride, trichlorethylene, liquid carbon dioxide, amyl alcohol, or mixtures thereof.  According to an example, air-dried rootlets are extracted with a mixture of acetone and petroleum spirit.  The extract is concentrated under reduced pressure to the consistency of a syrup, mixed with hot alcohol, and, if desired after filtration from the undissolved portion, treated with alcoholic soda or potash, or sodium alcoholate.  The soaps formed are precipitated with alcoholic calcium chloride solution and removed by filtration.  The solution is freed from alcohol by evaporation under reduced pressure, the residue extracted with warm acetone, the acetone evaporated, the residue redissolved in acetone, and the solution cooled to 20 DEG  C. in an atmosphere of dry nitrogen saturated with acetone vapour.  The precipitated mass is washed with acetone and then extracted with warm petroleum spirit. The extract thus obtained is frozen out at -20 DEG C., and the crystals obtained aspirated in nitrogen and washed in acetone at -20 DEG  C.  These crystals and finally the residue left on evaporating the petroleum spirit mother liquor are dried in vacuo, dissolved in ethyl alcohol freed from air by means of nitrogen, and the solutions kept in hermetically sealed bottles. Specification 328,942, [Class 14 (ii), Beverages &c.], is referred to.  The Specification as open to inspection under Sect. 91 (3) (a) comprises also another example, in which the insoluble soap is washed with ether, and the washings added to the alcoholic solution separated from the soap.  This subject-matter does not appear in the Specification as accepted.