Patent RU-2708337-C2 (RU)
Title: Methods and compositions for dna profiling
Inventor: STEFENS KETRIN M
IPC: C12Q1/6827
Filed: 2015-02-13
Granted: 2019-12-05
Abstract: FIELD: biotechnologies. 
     SUBSTANCE: invention relates to the biotechnology. Described is a plurality of primers for use in constructing a human DNA profile, comprising two sets of primers. First set of primers contains primers which specifically hybridise with the first target sequence and in combination with DNA polymerase and nucleotides amplify a short amplification product, having a short amplification product length, wherein the short amplification product sequence comprises single nucleotide polymorphism (SNP), where the first set of primers contains one or more primers, each comprising a nucleic acid sequence presented by any of SEQ ID NO: 111–402. Second set of primers contains primers which specifically hybridise with the second target sequence and in combination with the DNA polymerase and nucleotides amplify a long amplification product having a long amplification product length, where the length of the short amplification product is shorter than the length of the long amplification product, where the sequence of the long amplification product contains a tandem repeat, where the second set of primers contains one or more primers, each comprising a nucleic acid sequence presented by any of SEQ ID NO: 1–110. At least one of the primers in the plurality of primers has a melting point which is less than 60 °C, or has a length of at least 24 nucleotides or contains a homopolymeric nucleotide sequence or a combination thereof, where at least one of the primers in the plurality of primers has a melting point which is less than 60 °C, and has a length of at least 24 nucleotides and where a plurality of primers comprises a first set of primers comprising up to 20,000 primers, and a second set of primers comprising up to 1200 primers. Described is a method of constructing a DNA profile, involving: providing a nucleic acid sample, amplifying a nucleic acid sample with a plurality of primers which are specifically hybridised with at least one target sequence comprising mononucleotide polymorphism (SNP), and with at least one target sequence containing a tandem repeat, in a multiplex reaction to obtain amplification products, where at least one of the primers in the plurality of primers has a melting point which is less than 60 °C, or has a length of at least 24 nucleotides, or contains a homopolymeric nucleotide sequence or a combination thereof, where at least one of the primers in the plurality of primers has a melting point which is less than 60 °C and has a length of at least 24 nucleotides, and determining genotyping amplification of at least one SNP and at least one tandem repeat, thereby obtaining a DNA profile of the nucleic acid sample. In addition, a method of constructing a library of nucleic acids, comprising: providing a nucleic acid sample, and amplifying a nucleic acid sample with a plurality of primers which are specifically hybridised with at least one target sequence comprising mononucleotide polymorphism (SNP), and with at least one target sequence containing a tandem repeat sequence, in a multiplex reaction to produce amplification products, where at least one of the primers in the plurality of primers has a melting point which is less than 60 °C, or has a length of at least 24 nucleotides, or contains a homopolymer nucleotide sequence or a combination thereof, wherein at least one of the primers in the plurality of primers has a melting point which is less than 60 °C and has a length of at least 24 nucleotides. 
     EFFECT: invention enables to obtain a human DNA profile. 
     32 cl, 25 dwg, 5 tbl, 7 ex