Patent SG-11202000444P-A (SG)
Title: Sequencing output determination and analysis with target-associated molecules in quantification associated with biological targets
Inventor: TSAO DAVID
IPC: C12Q1/68
Filed: 2018-08-06
Abstract: performingone or more sequencing operations, such as based on the one or more spike-in mixtures S determining one or more abundance metrics, such as based on the one or more sequencing operations for the one or more spike-in mixtures S140 facilitating characterization of one or more conditions (e.g., based on the one or more abundance metrics) S150 r (12) INTERNATIONAL APPLICATION PUBLISHED (19) World Intellectual Property Organization International Bureau UNDER THE PATENT COOPERATION TREATY (PCT) vitivio VIII oviolo v1111 tomo vimiE (10) International Publication Number WO 2019/028470 A2 (43) International Publication Date 07 February 2019 (07.02.2019) WIP0 I PCT (72) Inventors: TSAO, David; 2627 Hanover Street, Palo Alto, CA 94304 (US). ATAY, Oguzhan; 2627 Hanover Street, Palo Alto, CA 94304 (US). (74) Agent: SCHOX, Jeffrey; 500 3rd Street, Suite 215, San Francisco, CA 94107 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (51) International Patent Classification: C12Q 1/6869 (2018.01) (21) International Application Number: PCT/US2018/045419 (22) International Filing Date: 06 August 2018 (06.08.2018) English English (25) Filing Language: (26) Publication Language: (30) Priority Data: 62/541,565 04 August 2017 (04.08.2017) US (71) Applicant: BILLIONTOONE, INC. [US/US]; 2627 Hanover Street, Palo Alto, CA 94304 (US). (54) Title: SEQUENCING OUTPUT DETERMINATION AND ANALYSIS WITH TARGET-ASSOCIATED MOLECULES IN QUANTIFICATION ASSOCIATED WITH BIOLOGICAL TARGETS r validating one or more sample processing and/or computational processing approaches S105 processing a set of reference- associated molecules S115 generating a set of target-associated molecules associated with one or more biological targets S110 generating at least one spike-in mixture (e.g., based on the set of target-associated molecules, the set of reference-associated molecules, and one or more biological samples) S120 repeat with additional biological sample(s); for any suitable conditions facilitating treatment for the one or more conditions (e.g., based on the one or more abundance metrics) S160 FIGURE 1A (57) : Embodiments of a method and/or system can include generating a set of target- associated molecules (e.g., spike-in molecules) associated with one or more biological targets; generating one or more spike-in mixtures based on processing the set of target- associated molecules with one or more samples including the one or more biological targets; performing one or more Sanger sequencing operations on the one or more spike- in mixtures; determining one or more abundance metrics based on chromatogram- related outputs from the one or more Sanger sequencing operations; and/or facilitating characterization of one or more medical conditions based on the one or more abundance metrics. C N 00 C 1-1 C [Continued on next page] WO 2019/028470 A2 MIDEDIMOHIMIRDEIHNIMUMEHMEDIMIE (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: without international search report and to be republished upon receipt of that report (Rule 48.2(g))