Patent SG-11201909644Q-A (SG)
Title: Fluorescent compounds specific for pluripotent stem cells and reprogramming-ready cells and methods of using the same
Inventor: SRIRAM SANDHYA
IPC: G01N33/533
Filed: 2018-07-04
Abstract: R 0) q F (R7)p W O 20 19/0098 10 Al Rio (I) N R11 (R2)m N, B \" N F F (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 10 January 2019 (10.01.2019) WIPO I PCT omit VIII VIII 10 o III IIIII 11o1H H 010 11011111° oimIE (10) International Publication Number WO 2019/009810 Al (51) International Patent Classification: C07F 5/02 (2006.01) G01N 33/533 (2006.01) C09B 57/00 (2006.01) (21) International Application Number: PCT/SG2018/050329 (22) International Filing Date: 04 July 2018 (04.07.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 10201705493P 04 July 2017 (04.07.2017) SG (71) Applicant: AGENCY FOR SCIENCE, TECHNOLO- GY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10, Connexis North Tower, Singapore 138632 (SG). (72) Inventors: SRIRAM, Sandhya; c/o Singapore Bioimaging Consortium, 11 Biopolis Way, #01-02, Helios, Singapore 138667 (SG). SUGII, Shigeki; c/o Singapore Bioimag- ing Consortium, 11 Biopolis Way, #01-02, Helios, Singa- pore 138667 (SG). CHANG, Young Tae; c/o Singapore Bioimaging Consortium, 11 Biopolis Way, #01-02, Helios, Singapore 138667 (SG). KANG, Nam-Young; c/o Singa- pore Bioimaging Consortium, 11 Biopolis Way, #01-02, Helios, Singapore 138667 (SG). (74) Agent: VIERING, JENTSCHURA & PARTNER LLP; P.O. Box 1088, Rochor Post Office, Rochor Road, Singa- pore 911833 (SG). = (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) in black and white; the international application as filed contained color or greyscale and is available for download from PATENTSCOPE (54) Title: FLUORESCENT COMPOUNDS SPECIFIC FOR PLURIPOTENT STEM CELLS AND REPROGRAMMING-READY CELLS AND METHODS OF USING THE SAME (57) : The present invention provides fluorescent compounds of formula (I) as disclosed herein or pharmaceutically acceptable salts there- of. Further provided are uses of said fluorescent compounds in methods of determining, in a sample, the presence and/or amount of pluripotent stem cells or cells undergoing reprogramming to become induced pluripotent stem cells.