Patent SG-11201807544W-A (SG)
Title: Improved muconic acid production from genetically engineered microorganisms
Inventor: SILLERS RYAN
IPC: C12P7/44
Filed: 2017-03-01
Abstract: FigurearoG, aroF, aroH Quinnic Acid 14— Tyr-Phe-Trp pobA PCA DAHP pobA Gallic Acid aroY Pyrogallol rr aroD DHS 4 Leaky aroE SHK rr aroK, aroL S3P CHA 4, aroY Catechol 4, cat4 ccMuA 4 , lsomerase ttMuA Gallic Acid 4, aro) . Pyrogallol aroZ W O 20 17 / 15 18 11 Al (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/151811 Al 8 September 2017 (08.09.2017) WIPO I PCT 11111111111111 0 111011111111111 010 11111 III 011111111 H 01111111 011 (51) International Patent Classification: C12N 1/21 (2006.01) C12P 7/44 (2006.01) C12N 15/09 (2006.01) C12N 15/60 (2006.01) C12N 15/52 (2006.01) (21) International Application Number: PCT/US2017/020263 (22) International Filing Date: 1 March 2017 (01.03.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/302,558 2 March 2016 (02.03.2016) US (71) Applicant: MYRIANT CORPORATION [US/US]; 42 Cummings Park, Woburn, MA 01801 (US). (72) Inventors: SILLERS, Ryan; 17 Forest Circle, Winchester, MA 01890 (US). SPENCER, Michelle; 82 Oak Hill Street, Pepperell, MA 01463 (US). YOCUM, R., Rogers; 4 Orchard Street, Lexington, MA 02420 (US). HER- MANN, Theron; 151 Warren Street, Arlington, MA 02474 (US). (74) Agent: MANNAN, Ramasamy, Mannar; Myriant Cor- poration, 42 Cummings Park, Woburn, MA 01801 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). [Continued on next page] (54) Title: IMPROVED MUCONIC ACID PRODUCTION FROM GENETICALLY ENGINEERED MICROORGANISMS (57) : The subject of this inven- tion is improvements in the yield and titer of biological production of muconic acid by fermentation. Increased activity of one or more enzymes involved in the muconic acid pathway leads to increased production of muconic acid. WO 2017/151811 Al 1#1101M011111 11111111ME3011111111111111111111111111111111111 Published: with international search report (Art. 2 1 (3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) — with sequence listing part of description (Rule 5.2(a))