Patent SG-11201909394P-A (SG)
Title: Compositions and methods for improving sample identification in indexed nucleic acid libraries
Inventor: VERMAAS ERIC
IPC: C12Q1/6806
Filed: 2018-04-23
Abstract: Denatureand Anneal Extension W O 18/ 200386 A8 Extension FIG. 1 (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) CORRECTED VERSION (19) World Intellectual Property Organization International Bureau (43) International Publication Date 1111111111110110101011111011110111111010 11111111111MHERE 1111111# (10) International Publication Number WO 2018/200386 A8 01 November 2018 (01.11.2018) WIPO I PCT (51) International Patent Classification: C12Q 1/6806 (2018.01) C12Q 1/6855 (2018.01) (21) International Application Number: PCT/US2018/028881 (22) International Filing Date: 23 April 2018 (23.04.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/488,833 23 April 2017 (23.04.2017) US (71) Applicants: ILLUMINA, INC. [US/US]; 5200 Illumina Way, San Diego, CA 92122 (US). ILLUMINA CAM- BRIDGE LIMITED [GB/GB]; Nr Saffron Walden, CB10 1XL (GB). (72) Inventors: VERMAAS, Eric, Hans; 5200 Illumina Way, San Diego, CA 92122 (US). KHOSROHEIDARI, Mandieh; 5200 Illumina Way, San Diego, CA 92122 (US). BEVIS-MOTT, Claire; Nr Saffron Walden, CB10 1XL (GB). (74) Agent: CAMPBELL, Keith M.; Mueting, Raasch & Gebhardt, P.A., P.O. Box 581336, Minneapolis, MN 55458-1336 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (54) Title: COMPOSITIONS AND METHODS FOR IMPROVING SAMPLE IDENTIFICATION IN INDEXED NUCLEIC ACID LIBRARIES (57) : The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by modifying or blocking 5' and 3' ends of pooled indexed polynucleotides from multiple samples, with an optional exonuclease treatment, prior to amplification and sequencing. [Continued on next page] WO 2018/200386 A8 MIDEDIMOMOIDEIRDE1010010101111110MEROME#110# TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(11)) Published: — with international search report (Art. 21(3)) (48) Date of publication of this corrected version: 14 February 2019 (14.02.2019) (15) Information about Correction: see Notice of 14 February 2019 (14.02.2019)