Patent DE-10135051-A1 (DE)
Title: Preparing L-amino acids, e.g., L-threonine, by fermenting microorganisms of Enterobacteriaceae family in which genes e.g. malE, phoA, phoB, phoR are attenuated, in particular eliminated, and isolating L-amino acid
Inventor: HERMANN THOMAS
IPC: C12N15/31
Filed: 2001-07-18
Abstract: Preparing (M) L-amino acids, e.g. L-threonine comprising fermenting microorganisms of Enterobacteriaceae family in which one or more of genes malE, phoA, phoB, phoR, phoE, phnC to phnG, phnJ to phnP, pykF, pfkB, eda, talB, rpiB, zwf, mopA, pstA to pstC, pstS, ugpA, ugpE, ugpC, ugpQ, dnaK, dnaJ, clpB, rpoE, rseA, rseC, htpG, sodA, ompF, ompC, sucA to sucD, gltA and sdhB, or the sequence coding for it, is attenuated, is new. (M) comprises fermenting microorganisms of the Enterobacteriaceae family which produce the desired L-amino acid and in which one or more of the genes malE (periplasmic binding protein for maltose transport), phoA (alkaline phosphatase), phoB (regulatory protein of the pho regulon), phoR (sensor protein of the pho regulon), phoE (pore protein E of the outer cell membrane), phnC (ATP-binding protein of the alkyl phosphonate transport system), phnD (substrate-binding protein of the alkyl phosphonate transport system), phnE (permease protein of the alkyl phosphonate transport system), phnF (putative regulatory protein), phnG, phnJ, phnM and phnP (membrane-bound subunit of the carbon-phosphorus lyase complex), phnK, phnL and phnN (ATP-binding proteins of the phosphonate transporter), phnO (putative regulator of the carbon-phosphorus lyase complex), pykF (fructose-stimulated pyruvate kinase I), pfkB (6-phosphofructokinase isoenzyme 2), eda (2-keto-3-deoxygluconate 6-phosphate aldolase and 2-keto-4-hydroxyglutarate aldolase (Entner-Doudoroff aldolase)), talB (transaldolase B), rpiB (ribose 5-phosphate isomerase B), zwf (glucose 6-phosphate 1-dehydrogenase), mopA (Chaperone GroEL, heat shock protein Hsp60), pstA (permease protein of the high-affinity phosphate transport system), pstB (ATP binding component of the high-affinity phosphate transport system), pstC (permease protein of the high-affinity phosphonate transport system), pstS (periplasmic phosphate-binding protein of the high-affinity phosphate transport system), ugpA and ugpE (permease protein of the sn-glycerol 3-phosphate transport system), ugpC (ATP-binding component of the sn-glycerol 3-phosphate transport system), ugpQ (glycerol phosphodiester phosphodiesterase), dnaK (autoregulated heat shock protein Hsp70), dnaJ (Chaperone, heat shock protein), clpB (heat shock protein f84.1), rpoE (\=s-E subunit of RNA polymerase), rseA (membrane protein with anti-\=sE activity), rseC (regulatory protein of the \=sE factor), htpG (Chaperone, heat shock protein c62.5), sodA (superoxide dismutase), ompF (outer membrane protein F), ompC (outer membrane protein C), sucA (decarboxylase subunit of 2-ketoglutarate dehydrogenase), sucB (dihydrolipoyltranssuccinase subunit of 2-ketoglutarate dehydrogenase), sucC ( beta -subunit of succinyl-CoA synthetase), sucD ( alpha -subunit of succinyl-CoA synthetase), gltA (citrate synthase) and sdhB (iron-sulfur protein subunit of succinate dehydrogenase), or the nucleotide sequence which codes for this, is or are attenuated, in particular eliminated, concentrating the desired L-amino acid in the medium or in the cells of the microorganisms, and isolating the desired L-amino acid, constituents of the fermentation broth and/or the biomass in its entirety or its portions (greater than 0 to 100%) optionally remaining in the product.