Patent SG-11201903189P-A (SG)
Title: Device, system and method for intracellular delivery of substances
Inventor: SEOW YIQI
IPC: C12N15/87
Filed: 2017-10-20
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property MD 1101111 0 DOI HIM 011101 0 011111111111111111111111111111111111111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\" WO 2018/074980 Al 26 April 2018 (26.04.2018) WIP0 I PCT (51) International Patent Classification: DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, C12N 15/87 (2006.01) HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, (21) International Application Number: KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, PCT/SG2017/050527 MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (22) International Filing Date: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, 20 October 2017 (20.10.2017) TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (25) Filing Language: English (84) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (30) Priority Data: UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, 10201608846Y 21 October 2016 (21.10.2016) SG TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FL FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (71) Applicant: AGENCY FOR SCIENCE, TECHNOLO- MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, GY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, #20-10 Connexis, Singapore 138632 (SG). KM, ML, MR, NE, SN, TD, TG). (72) Inventors: SEOW, Yiqi; C/O Biomedical Science Insti- tute, 20, Centros, Biopolis Way, Singapore 138668 (SG). Declarations under Rule 4.17: BEH, Weijie Cyrus; C/O Biomedical Science Institute, 20, — of inventorship (Rule 4.17(iv)) — Centros, Biopolis Way, Singapore 138668 (SG). Published: (74) Agent: DONALDSON & BURKINSHAW LLP; 24 Raf- — with international search report (Art. 21(3)) = fles Place, #15-00, Clifford Centre, Singapore 048621 (SG). (81) Designated States (unless otherwise indicated, for every = _ kind of national protection available): AE, AG, AL, AM, = AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (54) Title: DEVICE, SYSTEM AND METHOD FOR INTRACELLULAR DELIVERY OF SUBSTANCES = (57) : The invention relates to an attachment configured to detach- ably couple to a fluid extruding device, the attachment comprising: a mem- — = — 804 800 ../ 902 8 \" branhaving a plurality of pores configured to allow a cell to pass through while inducing a mechanical stress to the cell such that the introduction of one or more substances into the cell is facilitated; and an engaging mem- ber for coupling to the outlet of the fluid extruding device such that the membrane is in fluid communication with the outlet of the fluid extruding device, wherein the fluid extruding device is configured to extrude fluid via mechanical actuation. The fluid extruding device may include a pipette, = — - = = = micropipette, burette, syringe or liquid handling system. The pores of said membrane are preferably linear through the membrane and have an aver- age pore diameter of 40% to 70% of the average diameter of the cell. The = _ = = 802 E06 810 invention further includes methods of introducing substances into a cell, a fluid extruding device and a system thereof. 808 - 11 1 814 812 CZ GC -, 818 7r 816 Ir ... ' CZ \... :20 11 CZ ei Fig. 9 C