Patent SG-11201803958W-A (SG)
Title: Nucleic acids encoding repetitive amino acid sequences rich in proline and alanine residues that have low repetitive nucleotide sequences
Inventor: BINDER ULI
IPC: C12N15/64
Filed: 2016-12-22
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -' Organization International Bureau (43) International Publication Date .... ....7:01 29 June 2017(29.06.2017) WIPO I PCT (10) WO International 111111111111311111111111111111111111111111111111111111111111111111111111111111111111111111 2017/109087 Publication Al Number (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12N 15/62 (2006.01) C12N 15/11 (2006.01) kind of national protection available): AE, AG, AL, AM, A61K 38/00 (2006.01) C12N 15/64 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, C07K 14/00 (2006.01) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (21) International Application Number: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, ICH, KN, PCT/EP2016/082407 KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, (22) International Filing Date: MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, 22 December 2016 (22.12.2016) NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, (25) Filing Language: English TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, (26) Publication Language: English ZA, ZM, ZW. (30) Priority Data: (84) Designated States (unless otherwise indicated, for every 15202093.9 22 December 2015 (22.12.2015) EP kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, (71) Applicants: XL-PROTEIN GMBH [DE/DE]; Lise-Meit- TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, ner-Str. 30, 85354 Freising (DE). TECHNISCHE UNI- TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, VERSITAT MUNCHEN [DE/DE]; Arcisstras se 21, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, 80333 Miinchen (DE). LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, (72) Inventors: BINDER, Ulrich; Angerstr. 7, 85354 Freising SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (DE). ACHATZ, Stefan; Max-Lehner-Str. 24, 85354 Fre- ising (DE). SKERRA, Arne; Max-Lehner-Str. 19, 85354 Published: Freising (DE). 21(3)) — with international search report (Art (74) Agent: MEIER, Jurgen; Vossius & Partner, SiebertstraBe _ with sequence listing part of description (Rule 5.2(a)) 3, 81675 Miinchen (DE). 1-1 .4t N (54) Title: NUCLEIC ACIDS ENCODING REPETITIVE AMINO ACID SEQUENCES RICH IN PROLINE AND ALANINE GC RESIDUES THAT HAVE LOW REPETITIVE NUCLEOTIDE SEQUENCES 0 C(57) : The present invention relates to a nucleic acid molecule comprising a low repetitive nucleotide sequence encoding a • Il prolme/alanine-rich amino acid repeat sequence. The encoded polypeptide comprises a repetitive amino acid sequence that forms a *----- random coil. The nucleic acid molecule comprising said low repetitive nucleotide sequences can further comprise a nucleotide se - L -- quence encoding a biologically or pharmacologically active protein. Further, the present invention provides for selection means and ::::) methods to identify said nucleic acid molecule comprising said low repetitive nucleotide sequence. The present invention also relates ei to a method for preparing said nucleic acid molecules. Also provided herein are methods for preparing the encoded polypeptide or 0 drug conjugates with the encoded polypeptide using the herein provided nucleic acid molecules. The drug conjugate may comprise a biologically or pharmacologically active protein or a small molecule drug. Also provided herein are vectors and hosts comprising 5 such nucleic acid molecules.