Patent DE-102013010528-B3 (DE)
Title: Creating database for preliminary estimation of effectiveness of cytostatic active substance in chemotherapeutic treatment of tumor, by incubating tumor cells of previously removed tumor tissue sample from patients
Inventor: GRANZOW CHRISTOF
IPC: C12Q1/02
Filed: 2013-06-25
Granted: 2014-02-20
Abstract: Creating a database for a preliminary estimation of the effectiveness of a cytostatic active substance in the chemotherapeutic treatment of a tumor, preferably malignant tumor, a particular patient on the basis of ex vivo determination of the IC50 value of an active substance for tumor cells of a tumor tissue sample previously taken from the same patient, comprises: incubating the tumor cells (preferably malignant cells) of the previously removed tumor tissue sample from the patients (ex vivo) with the active substance; and determining the IC50 value of the drug in the cells. Creating a database for a preliminary estimation of the effectiveness of a cytostatic active substance in the chemotherapeutic treatment of a tumor, preferably malignant tumor, a particular patient on the basis of ex vivo determination of the IC50 value of an active substance for tumor cells of a tumor tissue sample previously taken from the same patient, comprises: incubating the tumor cells (preferably malignant cells) of the previously removed tumor tissue sample from the patients (ex vivo) with the active substance; determining the IC50 value of the drug in the cells; treating the same patient with the active substance according to current evidence-based therapy rules; and documenting the treatment outcome and classifying in the form of a classification with the classes I = complete remission of the tumor (VR), II = partial remission of the tumor (PR), III = zero growth of the tumor = no change (NC) and IV = progressive growth of the tumor = progress, where the documentation and/or classification of the resulting treatment effect is assigned with the ex vivo of determined IC50 value. An independent claim is included for preliminary estimation of the effectiveness of (cytostatic) active substance in the therapeutic treatment of the malignant tumor, a particular current patient on the basis of ex vivo determination of the IC50 value of an active substance for tumor cells of a tumor tissue sample previously taken from the same patient, where the tumor cells of the tumor tissue sample previously taken from the patient (ex vivo) are incubated with the active substance, and the IC50 value of the active substance is determined in the cells, comprising comparing the calculated IC50 value with previously determined IC50 values of the reference of the same active substance in the tumor cells of the same tumor entity, where each IC50 reference value is assigned with the treatment outcome, treating the same patient with the active substance according to current evidence-based therapy rules, and documenting the treatment outcome and classifying in the form of a classification with the classes I = complete remission of the tumor (VR), II = partial remission of the tumor (PR), III = zero growth of the tumor = no change (NC) and IV = progressive growth of the tumor = progress.