Patent BR-112015002484-A2 (BR)
Title: xilanases para solubilização de material contendo arabinoxilano
Inventor: BART CHRISTIAAN KOOPS
IPC: A23L7/20
Filed: 2013-08-02
Abstract: abstract (en)the present invention relates to a method for solubilising arabinoxylan-containing material (particularly insoluble arabinoxylan-containing material), comprising admixing a xylan- containing material with a xylanase comprising a polypeptide sequence shown herein as seq id no. 3, seq id no. 2, seq id no. 1, seq id no. 9, seq id no. 10, seq id no. 11 or seq id no. 15, or a variant, homologue, fragment or derivative thereof having at least 75% identity with seq id no. 3 or seq id no. 2 or seq id no. 1 or seq id no. 9 or seq id no. 10 or seq id no. 11 or seq id no. 15; or a polypeptide sequence which comprises seq id no. 3, seq id no. 2, seq id no. 1, seq id no. 9, seq id no. 10, seq id no. 11 or seq id no. 15 with a conservative substitution of at least one of the amino acids; or a xylanase which is encoded by a nucleotide sequence shown herein as seq id no. 6, seq id no. 5, seq id no. 4, seq id no. 12, seq id no. 13, seq id no. 14, seq id no. 16, seq id no.17 or seq id no. 18, or a nucleotide sequence which can hybridize to seq id no. 6, seq id no. 5, seq id no. 4, seq id no. 12, seq id no. 13, seq id no. 14, seq id no. 16, seq id no.17 or seq id no. 18 under high stringency conditions, or a nucleotide sequence which has at least 75% identity with seq id no. 6, seq id no. 5, seq id no. 4, seq id no. 12, seq id no. 13, seq id no. 14, seq id no. 16, seq id no.17 or seq id no. 18, or a nucleotide sequence which differs from seq id no. 6 or seq id no. 5 or seq id no. 4 or seq id no. 12 or seq id no. 13 or seq id no. 14 or seq id no. 16 or seq id no.17 or seq id no. 18 due to the degeneracy of the genetic code, or a xylanase obtainable (or obtained) from fusarium verticilloides. the present invention also relates to a novel xylanase comprising (or consisting of) a polypeptide sequence shown herein as seq id no. 3, seq id no. 2 or seq id no. 1, or a variant, homologue, fragment or derivative thereof having at least 99% identity with seq id no. 3 or seq id no. 2 or seq id no. 1; or a xylanase which is encoded by a nucleotide sequence shown herein as seq id no. 6, seq id no. 5 or seq id no. 4, or a nucleotide sequence which can hybridize to seq id no. 4 or seq id no. 5 under high stringency conditions, or a nucleotide sequence which has at least 97.7% identity (preferably 98% identity) with seq id no. 6, seq id no. 5 or seq id no. 4. the present invention yet further relates to methods relating to feedstuffs, malting and brewing, processing of grain-based materials such as during the production of bioethanol or biochemical (e.g. bio-based isopropanol), or wheat gluten-starch separation processes and the like. tradução do resumo resumo patente de invenção: "xilanases para solubilização de material contendo arabinoxilano". a presente invenção refere-se a um método para solubilização de material contendo arabinoxilano (particularmente material contendo arabinoxilano insolúvel), compreendendo mistura de um material contendo xilano com uma xilanase compreendendo uma sequência de polipeptídeo ou uma variante, homólogo, fragmento ou derivado do mesmo ou uma sequência de polipeptídeo que compreende uma substituição conservativa de pelo menos um dos aminoácidos; ou uma xilanase que é codificada por uma sequência de nucleotídeo ou uma sequência de nucleotídeo que pode hibridizar sob condições de alta adstringência ou uma xilanase obtenível (ou obtida) de fusarium verticilloides. a presente invenção refere-se ainda a métodos relacionados a gêneros alimentícios, maltagem e processo de produção de cerveja, processamento de materiais à base de grão tal como durante a produção de bioetanol ou bioquímico (por exemplo, isopropanol de biobase) ou processos de separação de glúten-amido de trigo e similar.