Patent AU-2022201189-A1 (AU)
Title: Modification of transcriptional repressor binding site in NF-YC4 promoter for increased protein content and resistance to stress
Inventor: LI LING
IPC: C12N15/82
Filed: 2022-02-22
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) 
(19) World Intellectual Property 
Organization 
International Bureau 
(10) International Publication Number 
(43) International Publication Date W O 2016/134081 A1 
25 August 2016 (25.08.2016) W IPOIPCT 
(51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every 
A01H 5/00 (2006.01) C12N 15/82 (2006.01) kind of national protection available): AE, AG, AL, AM, 
C07K14/415 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, 
.21) .nterntia ABZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, 
(21) InternationalApplicationNumber: DO, DZ, EC, EE, EG, ES, Fl, GB, GD, GE, GH, GM, GT, 
PCT/US2016/018358 HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, 
(22) International Filing Date: KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, 
17 February 2016 (17.02.2016) MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, 
PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, 
(25) Filing Language: English SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, 
(26) Publication Language: English TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.  
(30) Priority Data: (84) Designated States (unless otherwise indicated, for every 
62/117,924 18 February 2015 (18.02.2015) US kind of regional protection available): ARIPO (BW, GH, 
62/244,131 20 October 2015 (20.10.2015) US GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, 
TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, 
(71) Applicant: IOWA STATE UNIVERSITY RESEARCH TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, 
FOUNDATION, INC. [US/US]; 310 Lab of Mechanics, DK, EE, ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, 
Ames, Iowa 50011-2131 (US). LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, 
(72) Inventors: LI, Ling; 906 Tennyson Avenue, Ames, Iowa SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, 
50014 (US). WURTELE, Eve Syrkin; 3425 Oakland GW, KM, ML, MR, NE, SN, TD, TG).  
Street, Ames, Iowa 50014 (US). Published: 
(74) Agent: LARCHER, Carol; Larcher & Chao Law Group, - with international search report (Art. 21(3)) 
P.O. Box 1666, Skokie, Illinois 60076 (US). - with sequence listing part ofdescription (Rule 5.2(a)) 
(54) Title: MODIFICATION OF TRANSCRIPTIONAL REPRESSOR BINDING SITE IN NF-YC4 PROMOTER FOR IN
CREASED PROTEIN CONTENT AND RESISTANCE TO STRESS 
(57) Abstract: Method of increasing protein content in a eukaryotic cell comprising an NF-YC4 gene comprising modifying the 
transcriptional repressor binding site; method of producing a plant with increased protein content comprising crossing and selecting 
for increased protein content; method of increasing resistance to a pathogen or a pest in a plant comprising an NF-YC4 gene com 
prising modifying the transcriptional repressor binding site, alone or in further combination with expressing QQS in the plant; meth
N4 od for producing a plant with increased resistance to a pathogen or a pest comprising crossing and selecting for increased resistance 
to the pathogen or the pest; a cell, collection of cells, tissue, organ, or organism in which the NF-YC4 gene comprises a promoter 
comprising a transcriptional repressor binding site that has been modified so that the transcriptional repressor cannot prevent tran 
scription of the NF-YC4; hybrid plants; and seeds.