Patent SG-11201407729V-A (SG)
Title: Methods for drug screen using zebrafish model and the compounds screened thereform
Inventor: WANG I-JONG
IPC: C07C259/04
Filed: 2013-05-21
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 28 November 2013 (28.11.2013) WIPOIPCT (10) International Publication Number WO 2013/177170 A3 (51) International Patent Classification: A61K31/16 (2006.01) C07C 259/04 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/US2013/04203 7 21 May 2013 (21.05.2013) English English (30) Priority Data: 61/649,611 21 May 2012 (21.05.2012) US (71) Applicants: DCB-USA LLC [US/US]; 1007 North Or­ ange Street, 9th Floor, New Castle County, Wilmington, DE 19801 (US). NATIONAL TAIWAN UNIVERSITY; No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 (TW). (72) Inventor: WANG, I-Jong; No. 1, Changde St., Zhong- zheng Dist., Taipei City, 10048 (TW). (74) Agent: KING, Anthony; WPAT, P.C. Intellectual Prop­ erty Attorneys, 1100 Quail Street, Suite 202, Newport Beach, CA 92660 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) (88) Date of publication of the international search report: 13 February 2014 o i> (54) Title: METHODS FOR DRUG SCREEN USING ZEBRAFISH MODEL AND THE COMPOUNDS SCREENED THERE- i-H FORM i-H o CJ (57) Abstract: The invention relates to a platform of using zebrafish in screening candidates for treating and/or preventing myopia and keratoconus disease. The invention is mainly based on that Lumican, one of several SLRPs, plays an important role in the regu - lation of fibrillogenesis or the genes affecting the size of eyeballs in zebrafish, in addition to playing an important role in clinical my­ opia. Therefore, the invention uses the established zebrafish model to further identify the drugs affecting the expression of lumican and collagen fibrillogenesis, and/or the regulation of eyeball size. These drugs are potential candidates for treating myopia and/or keratoconus disease.