Patent RU-2643256-C1 (RU)
Inventor: BOGDANOV NIKOLAJ IVANOVICH
IPC: A01G33/00
Filed: 2016-10-26
Granted: 2018-01-31
Abstract: FIELD: biotechnology. 
     SUBSTANCE: installation contains aquariums of transparent material arranged on frame, interconnected by a pipeline including drain holes connected to the finished suspension container and the nutrient solution container, and luminaires placed between the aquariums. There are three aquariums for suspension on the frame, the total volume of the two side aquariums is equal to the volume of the central one, the lamps are located vertically between the side aquariums and horizontally above the central container. All of them are united in a luminaire, consisting of a single frame fixed to the stand frame, with a possibility of its transfer to a vertical position when maintaining the aquariums and switching to sunlight. Containers for nutrient solution preparation are exposed at a level above the aquariums, and each aquarium has a separate container with the corresponding volume. In the bottom wall of each container has an opening to which pipelines for nutrient solution draining are connected. Each aquarium has three openings, one of which serves to balance the suspension volumes, the second - to drain the finished suspension, the third is located in the lower plane and is designed to remove bottom sediments and washing water while washing the aquarium, and a container for the nutrient solution. Aquariums and the container for collection and storage of finished products are interconnected by a pipeline. In the method, chlorella strains are grown using a light source, optimal nutrient medium and temperature conditions. Optimal lighting in the initial period of cultivation is achieved by vertically located lamps between the aquariums. During the growth of cells and increase of the optical density of the culture, horizontal lamps located above the central aquarium are connected. The culture which has reached the normative optical density, is poured alternately, once from the central aquarium, next time from two side containers. The original culture flows into the empty aquarium after draining the culture through the connecting pipeline, after which a nutrient solution is added. Stock culture production is integrated into a single biotechnological process, which is carried out alternately in the central or side containers. Cultivation is carried out in different regimes using separate plankton strains: Chlorella vulgaris IGF C-111, Chlorella vulgaris BIN, Chlorella kessleri VKPM Al-12 and nutrient solution optimal for each of them in terms of 1 l of tap water: Mode I: Culture of Chlorella vulgaris IGF C111 strain, block 1: Nitric-phosphorus solution 0.3 ml, copper-cobalt solution 0.5 ml; block 2: Solution of carbon dioxide obtained using a material containing fiber (straw or wheat germ), pH 6.0-7.0, 10.0 ml. Mode II: Culture of Chlorella vulgaris BIN strain, block 1: Nitric-phosphorus solution 0.6 ml, copper-cobalt solution 1.0 ml, block 2: Solution of carbon dioxide obtained using a grain material (wheat, holer oats), pH 5.0-6.0, 5.0-10.0 ml. Mode III: Culture of Chlorella vulgaris VKPM Al-12 strain, block I: Nitric-phosphoric solution 0.9 ml, Copper-cobalt solution 1.5 ml, Potassium sulphate solution 1.0 ml, block 2; Solution of carbon dioxide obtained with using grain material (holer oats), pH 5.0-6.0, 30.0 ml, intended for obtaining: according to Mode I - fodder for farm animals, according to Mode II - reservoir algolizant, according to Mode III - chlorella biomass. 
     EFFECT: inventions allow to optimize cultivated crops lighting. 
     2 cl, 3 dwg, 3 ex