Patent SG-11201908240Y-A (SG)
Title: Methods for evaluating tumor cell spheroids using 3d microfluidic cell culture device
Inventor: JENKINS RUSSELL
IPC: A61K49/16
Filed: 2018-03-30
Abstract: MicrofluidicD Culture Serial Microscopy (Live/Dead Analysis) Tumor Specknen Sequential filtration Physical+ enzymatic dissociation. Flow cytometry 105 CD6 T cells 104 1 10 01 -10- -10 3 0 10 3 10 4 10 3 -1 \" 1CD4 T cells 56.9 10 4 10 5 Lyrrionacytes 19.3 lexCytokine P iling of Conditioned Media FIG. 1A Murine- derived Patient- derived GO Go Go 00 O C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) CORRECTED VERSION (19) World Intellectual Property Organization International Bureau (43) International Publication Date 111111111111011010101111101111010 I011111101111111100HORIOVE# (10) International Publication Number WO 2018/183839 A8 04 October 2018 (04.10.2018) Wi PO I PCT (51) International Patent Classification: Lane, Andover, MA 01810 (US). PAWELETZ, Cloud, P.; 9618 3 9 / 3 95 (2006.01) A61K 49/06 (2006.01) 725 Harrison Ave #404, Boston, MA 02118 (US). IVANO- 9618 49/00 (2006.01) 9618 4 9 / 1 6 (2006.01) VA, Elena; 100 Marion Street, #27, Brookline, MA 02446 9618 49/04 (2006.01) 9618 4 9 / 1 8 (2006.01) (US). AREF, Amir; 179 Gerry Road, Chestnut Hill, MA (21) International Application Number: PCT/US2018/025390 (22) International Filing Date: 30 March 2018 (30.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/480,192 31 March 2017 (31.03.2017) US (71) Applicant: DANA-FARBER CANCER INSTITUTE, INC. [US/US]; 450 Brookline Avenue, Boston, MA 02215 (US). (72) Inventors: JENKINS, Russell, W.; 1 Canal Street, Unit 626, Boston, MA 02114 (US). BARBIE, David; 2 Harwich 02467 (US). (74) Agent: BERKEY, Cristin; Wolf, Greenfield & Sacks, P.C., 600 Atlantic Avenue, Boston, MA 02210-2206 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (54) Title: METHODS FOR EVALUATING TUMOR CELL SPHEROIDS USING 3D MICROFLUIDIC CELL CULTURE DEVICE (57) : Provided herein are methods for evaluating tumor cell spheroids in a three-dimensional microfluidic device by deter- mining changes in the relative levels of live cells and dead cells in aliquots cultured under different conditions. Methods described herein allow ex vivo recapitulation of the tumor microenvironment such that the in vivo effectiveness of a test compound in treating tumor tissue may be predicted. [Continued on next page] WO 2018/183839 A8 MIDEDIM01101 DIONER1311111111111111111111111111111111110111111111111 (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) (48) Date of publication of this corrected version: 08 November 2018 (08.11.2018) (15) Information about Correction: see Notice of 08 November 2018 (08.11.2018)