Patent SG-11201805119Q-A (SG)
Title: Methods to determine tumor gene copy number by analysis of cell-free dna
Inventor: ELTOUKHY HELMY
IPC: G01N33/48
Filed: 2016-12-16
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 11111111011110111010101111101011111011101011011111111011101111101111011111 International Bureau ... .... ..Yjd ..... ...,/ (10) International Publication Number (43) International Publication Date WO 2017/106768 Al 22 June 2017(22.06.2017) WIPO I PCT (51) (21) (22) (25) Filing Language: (26) Publication Language: (30) (71) Applicant: GUARDANT HEALTH, (72) International Patent Classification: C12Q 1/68 (2006.01) GOOF 19/10 C12M 1/34 (2006.01) GO1N 33/48 International Application Number: International Filing Date: 16 December Priority Data: 62/269,051 17 December 2015 (17.12.2015) Penobscot Dr., Redwood City, California 94063 (US). Inventors: ELTOUKHY, Helmy; 2 Barry California 94027 (US). TALASAZ, AmirAli; ino a Los Cerros, Menlo Park, California CHUDOVA, Darya; 5931 Taormino California 95123 (US). ABDUEVA, Road, Orinda, California 94563 (US). Agent: AMODEO, Gabriele A.; Wilson & Rosati, 650 Page Mill Road, Palo Alto, (US). Designated States (unless otherwise kind of national protection available): AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (74) (81) PCT/US2016/067356 BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (2011.01) DO, DZ, EC, EE, EG, ES, FI, GB, (2006.01) HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, 2016 (16.12.2016) TH, TJ, TM, TN, TR, TT, TZ, ZA, ZM, ZW. English (84) Designated States (unless otherwise English kind of regional protection available): GM, KE, LR, LS, MW, MZ, US TZ, UG, ZM, ZW), Eurasian (AM, TJ, TM), European (AL, AT, BE, INC. [US/US]; 505 DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, Lane, Atherton, GW, KM, ML, MR, NE, SN, TD, 2181 Cam- GD, GE, GH, GM, GT, LR, LS, LU, LY, MA, UA, UG, US, UZ, VC, VN, indicated, for every ARIPO (BW, GH, NA, RW, SD, SL, ST, SZ, AZ, BY, KG, KZ, RU, BG, CH, CY, CZ, DE, TG). apply for and be granted a to claim the priority of the (Art. 21(3)) 94025 (US). Declarations under Rule 4.17: Avenue, San Jose, — as to applicant's entitlement to Diana; 227 Orchard patent (Rule 4.17(H)) — as to the applicant's entitlement Sonsini Goodrich earlier application (Rule 4.17(iii)) California 94304 Published: indicated, for every — with international search report AE, AG, AL, AM, Title: METHODS TO DETERMINE TUMOR GENE COPY NUMBER BY ANALYSIS OF CELL-FREE (54) DNA nucleic acid samples. These reduce variation due to _.— -I -- : Methods are provide improved approaches for determining baseline copy number of genetic loci within a sample, of genetic loci, sample preparation, and probe exhaustion. ,tD 0 N methods features 500 provided herein to improve automatic detection of copy number variation in z - 2 - 1000 1500 2000 2500 probes FIG. 15 ,-, GC ,tD IN © 1-1 IN Il (57)