Patent SG-11201810702V-A (SG)
Title: Humanized antibodies against enterovirus 71
Inventor: CREIGH-PULATMEN TILBE
IPC: C07K16/10
Filed: 2017-06-02
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 07 December 2017 (07.12.2017) WI P0 I PC T omit two Holonmomin Imo ow (10) International Publication Number WO 2017/207791 Al 1-1 1-1 N N N 1-1 O C (51) International Patent Classification: A61K 39/395 (2006.01) C07K 16/10 (2006.01) (21) International Application Number: PCT/EP2017/063535 (22) International Filing Date: 02 June 2017 (02.06.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 1609742.0 03 June 2016 (03.06.2016) GB (71) Applicant: LIFEARC [GB/GB]; 7th Floor, Lynton House, 7-12 Tavistock Square, London, Greater London WC1H 9LT (GB). (72) Inventors: CREIGH-PULATMEN, Tilbe; LifeArc, Open Innovation Campus, Gunnels Wood Road, Stevenage, Hert- fordshire, SG1 2FX (GB). PATEL, Seema; LifeArc, Open Innovation Campus, Gunnels Wood Road, Steve- nage, Hertfordshire, SG1 2FX (GB). MATTHEWS, David John; LifeArc, Open Innovation Campus, Gunnels Wood Road, Stevenage, Hertfordshire, SG1 2FX (GB). HUANG, Zhong; Institut Pasteur of Shanghai Chinese Academy of Sciences, Life Science Research Building, Room B407, 320 Yueyang Road, Shanghai 200031 (CN). (74) Agent: SUTCLIFFE, Nicholas et al.; Mewburn Ellis LLP, City Tower, 40 Basinghall Street, London, Greater London EC2V 5DE (GB). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, = (54) Title: HUMANIZED ANTIBODIES AGAINST ENTEROVIRUS 71 5azee mD5 vH 1 2 3 1234567890123456789012345679901234510367890123456789012ABC345678901234567890123456789012ABC345678901234567890AECDSIGHIjKIKNOPQRSTUV1234567890123 Sequence 4 5 6 1 9 9 10 11 TITALTSSNTAYLQLSSLTSESTAVYYCA.V.47117117!;;!; 777 TTLTVSS VQLQQSGAELVKPGASVKLSCTASGFNIHNEMINHVISQRPEQGLENIGIENT7 t r 17 8053 QVQLVQSCREVHSVGASVAVSOKASC;17 ltiVRQAPLX2RUNISOr 1 ITRUPSASTAYAISLSSIESEUSAVYYLASI, WGQ . M.I.AFEVSS D5 HA QVO SGASVMPGASVKVSCICASGY :! 'VRQAPGQRLEWIGL, 'TRLTSASTAYMELSSIRSEDTAVYYCAS ......... :-: .. .. , °7GQGTLVTVSS D5 ...), QVQLVQSGIEVSKPGASVKVSCKAS En: iiRQA PGQRLEN 'GI M alursvorAymussxa sEomv nue !!!:. wrounvss ID5 SG QVQINQSGAEVICKPGASVKVSCKA . - 4 , VRQAPGQiILSWI GI ' , :: :ITRIPSASTAYMEISSLASEDTAVYYCAS , ,,WGQGTLVTVSS HD DS_ HD QAPMRLICNIG pVTITADTSASTAYMELSSIRSEDTAVYYCAS' SS ,,,, fir QVQLVQSGA l'ASVNVSCRAS 2 JRQAPGQRLEWI( i. , . \"AVIITRDTSASTAYMELSSLASESTAVYYCA. DS HT QLVQSW1EVECKPGASVICVS.. VRQAPGQXLEW VTITACTSASTAINELSSLSSEDTAVVICA. SS DS HG D5 a , QVQLVQSGAEVYMPGASVRVSMSGYT QVQLVQSGFIEVISKPOSSVICOSCITASGY\":, ;;; ii,r7e0AiKIRT,D; , •:',QAPGQRLE , ; , :TITRU , SASTAIVELSSIRSEDTAVYYCA. :',1 — .AXTLVTVSS 2T/TIMTSASTAVICLSSIRSEDTAVITC7 , :V. ; , NTLVTVSS D53 ,, QVQ,LVQSOLEVK<PGASVSVSCICASGY • RQAPc4p.LF4 TITISTSASTIMISISSIASEDTAVT/C. D5f ij QVQLVQSM MS VVRQAPGQRLEHIGi , TRDTSASTAYMMSSLASEIRAVYYt .iwGQGT , vrvss 1)5 H DS HM D 5 H0 VQINQSSMITIZPGASVKVSCIS QVQINQSCALYKSPGASVKVSCIC. I7TC., QVULVQSGASVMPGASVAVSCAAS H r/VRQA PCVAL1011 3 '\" ,, VIQAPGQRLENIGNI 2QAPGVILEWIGL AYNEE,SMAISEDTAVTI • ,,I L VTVSS S AYMELSSIRSEDTAVYY '\"\"ENHEMENErlEMPONTLVTVSS YDELSSLASEDTAVYTC., SS D5_H QVQLVQS.AEK CK GOLD; ,. `TTSASTAYMELSSIASE VTVSS Figure 1 (57) : The present invention provides humanized antibodies against Enterovirus 71 (EV71), a causative agent of hand, foot, and mouth disease (HFMD), as well as pharmaceutical compositions comprising these antibodies and methods of their use in the treatment, prophylaxis, and diagnosis of HFMD. These antibodies are suitable for use in human subjects with HFMD or those at risk of HFMD, for example as a result of exposure to infected individuals. [Continued on next page] WO 2017/207791 Al IMEDIM0110101011M01011100101010E01111n0II1110ill0I# EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3))