Patent AU-2015271948-A1 (AU)
Title: Mutated protoporphyrinogen IX oxidase (PPX) genes
Inventor: GOCAL GREGORY F W
IPC: A01H5/00
Filed: 2015-12-18
Abstract: Provided are compositions and methods relating to gene and/or protein mutations in transgenic or non-transgenic plants. In certain embodiments, the disclosure relates to mutations in the protoporphyrinogen IX (PPX) gene. In some embodiments the disclosure relates to plants that 5 are herbicide resitant. Figure 2 iNcid sequence of Arabidopsis thaliona chloroplast protoporphyrinogen oxidase (P (Acsso- A X084732 4A 431) (SEQ ID NO: 1) 1 TGACAAATT CCGAATTCTC TGCGATTTLC AGGATGTAT CTTTTCGTCGCGC 61 CATCGTTC TCG1GTC TTALAGCC AATCTCCATDRVTGNITI 5 101 AGCTG AG CGTGGCCA ACGTATC AATG GG 151 GGAGGCACCALCCATCACGAGGATGGTG ATTGTCGGCG GA 251 ATCGCTCAGG Gt TGTC RAG CATCCT GALT CTEAISATGCTGC KVT 301 GC LGGTGGGGCAACA TVPS GAGLLRPL 10 351 GSAAGTA ATLYYATAV P ACTCDCELKGFGQLHPRTQGV 401 A 1 GC CGGTTCAIC T GA GSTN GILKSEGELVEA G ADRG 451 L:KMiiPNriSi PLK LCVRVW ,PQAI PQFLvGHFDILDIAKSSLI-SSGYEC 501 LGLGGGAAG A G TTALC GG TT T ACETAIVNGFGGRYAYK 15 Figure 2: Nucleotide sequence 01 Arabidopsis rhaliana chioroplas:protoporphyrinogen oxidaSC (PPOX -Al4g01690) (Accession 4 AX084732) (SEQ ID NO: 2) 1 TGACAAAATT CCAGATTCTC TGCGATTTCC ATGGGTTATG CTCTTCTCCG TCCGACGACT 61 CATAACCTTC TTCCGTCGTT TTCTAAGCCC AATCTCCGA'1 TAAATGTTTA A AAGCCTCTT 20 121 GGACTCCGTT GTTCAGTGGC CGGTGGACCA ACCGTCGGAT CTTCATAAAT CGAAGGCGGA 181 GGAGGCACCA CCATCACAC GGATTGTGTG ATTGTGGCG GAGGTATTAG TGGTCTTTGC 241 ATCGCTCAGC CGCTTGCTAC TAAGCATCC GA'PGCIGCTC CGAATTTAA AGACAC 301 GCTAAGGATC GTGTTGGAGG CAACATTATC ACTCTGAAG AGAATGGTAT TCTCTGGGAA 361 GAAGGCCCA ATAGTTTTCA ACCGTCTGAT CCTAOCTCA CTATGTTGT AGATAGTGGT 25 421 TGAAGATG ATTTGGTSTT GGGAGATCCT ACTGCGCCAA GGTTTGTGTT GCGGAATGGG 481 AAATTGAGGC CGATTCCATC CTAAGCTACA ACTTACCC TCTTTGATTT GATGAGTATT 541 GGGGGAAGA TTAGAGCTGG TTTTGGTGCA CTTGG2ATAC GACCGTCACC TCCAGGTCGT 601 GGGCAATCTG 'GGAGGAGTT TGThCGGCGT AACCTTAG ATGAGGTTTT CGAGCGCCTG 561 ATTGAACCGT TTTCGTTCAGG TATTTATCCT CGTATCCTGT CAAAACTGAG CATGAAAGCA 30 721 ACGTTTGGGA AGGTTTGGAA AQTAGACAA AATGGTGGA GCATAATAGG TSGTACTT T 781 AAGGCAATTC AGCAGAGAA AAACGCTCCC kAGGCAGAAC GAGACCCGCG CCTGCAAAA 841 CCACAGGGCC AACAGTTGC TTCTTTCAGG AAGGACTTC GAATGTTGCC AGAAGCAATA 101 TCTGCAAGAT TAGGTAGCAA AGTTAAGTTG TCTTTGGAC TCTCAGGTAT CACTAGCTG 161 GAGAGCGGAG GATACAACTT AACATATGAG ACTCCAGAG GTTAGTTTC CGTGCAGAGC 35 1021 ATG.CTCTTG TATGACGGT GCCATCTCAT GTCAAGTG GTCTCTTGCG CCCTGTTTCT 1081 GATCP2CTG CAAATGCACT CTCAAAACTA TATTACCCAC CAGTTGCAGC AGTATCTATC 141 TCGTACCCGA TAGAAGCTAT CCGAACAGAA TGTTTGATAG ATGGTGAAC AAAGGTTTT 1201 GGGCAATTGC ATCCACGCAC GCAAGGAGET GAAACATTAG GAACTA'PCTA CAGCTCCTCA 1261 CTCTTTCCAA ATCGCGCACC GCCCGGUGA ATTTTOGCTGT TGAACTACAT TGGCGGGTCT 40 1321 ACAAACACCG GAATTCTTC CAAGTCTAA mGTAGTTAG TGAAGCGT TGACAGAGAT 1381 TTGAGGAAAA TGCTA.A'TAA G2CTAAP'ICG ACCGATCCAC TT ATIAGG AGTTAGGGTA 1441 TGGCCTCAAG CCATTCCTCA GTTTCTAGTT CGTCACTT-TG PTATCCTTGA CACG(2CTAAA 1501 TCATCTCTAA CGCTTCGGG CTACGAAKGGG CTATTTTTG GTGGC TA CGTCGCTGGT i561 GTAGCCTTAG GCCG-GIGT AGAnAGIGCGlCA TA-TGA.AC2G COATTGAGOT CAACAACTTC 45 10621 ATGTCACGGT ACGCTTACAA GTIA1-GTAA VCATTAAAT CTCCCAGCTT GCGT'GAG'r'TT 1681. TATT'AXATAT TTTGAGATAT CCAAAAAAA. AAAAAAAA Figure 3: Aine acid sequence L, otkrab;dopxii rhaliana milochondrial protoporphyrinogen oxidase (At~g1 4220 50 1" ASAAHIASKVVGGVG;A~KR3VVE1R 51 VGGKL RSWIQNGI WDGANTM\TPVGSL LDDLGLREKQQFPISQKK 1 01 RT V!RNGVPWILP'TPIE VTSSVLSTQSKFQ tLE FLKKSSKVSDA 1 cD I, PPV <ADDIYSM7 55 F~~FI R>F~~<~i~