Patent SG-11201810031R-A (SG)
Title: Microorganism with modified aldehyde:ferredoxin oxidoreductase activity and related methods
Inventor: LIEW FUNGMIN
IPC: C12N1/20
Filed: 2017-05-12
Abstract: NADP)H AD(P). Ethanol NAD lactate .66H' ATP Synthas ADP 4. Pi V pyruvate CO 2 Acetolactate hr's a Acetoin 2,3BDHrAD(P)11 NADiPj• 2,3-Butanediol CoFeSP-[Chli 3 Acetyl-P ADP NAD(P)- Acetate Acetaldehyde Ald, AdhE NAD(P)H NADH N [CO] HADPH Fd Anabolism CO/ 4 1 4 e Fc‘,. x A N „ Acety-CoA Pf or Pyruvate NAD' 2NA PH NADH FIG. 1 A155 1-1 71- GC GC N O 1-1 C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 23 November 2017 (23.11.2017) WIPO I PCT o 1E1 olo VIII olommiolons H moimmio oimIE (10) International Publication Number WO 2017/200884 Al (51) International Patent Classification: C12N 1/20 (2006.01) Cl 2N 9/04 (2006.01) C12P 7/06 (2006.01) Cl 2N 9/02 (2006.01) (21) International Application Number: PCT/US2017/032564 (22) International Filing Date: 12 May 2017 (12.05.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/336,639 14 May 2016 (14.05.2016) US (71) Applicant: LANZATECH, INC. [US/US]; 8045 Lamon Avenue, Suite 400, Skokie, Illinois 60077 (US). (72) Inventors: LIEW, Fungmin; 8045 Lamon Avenue, Suite 400, Skokie, Illinois 60077 (US). KOEPKE, Michael; 8045 Lamon Avenue, Suite 400, Skokie, Illinois 60077 (US). (74) Agent: SCHOEN, Andrea E.; 8045 Lamon Avenue, Suite 400, Skokie, Illinois 60077 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (54) Title: MICROORGANISM WITH MODIFIED ALDEHYDE:FERREDOXIN OXIDOREDUCTASE ACTIVITY AND RELAT- ED METHODS (57) : The invention provides a non-naturally occurring bacterium having decreased or eliminated activity of an enzyme that catalyzes the reaction defined by EC 1.2.7.5, such as aldehyde:ferredoxin oxidoreductase (AOR). Optionally, the bacterium also has decreased or eliminated activity of an enzyme that catalyzes the reaction defined by EC 1.2.1.10 and/or EC 1.1.1.1, such as aldehyde dehydrogenase, alcohol dehydrogenase, or bifunctional aldehyde/alcohol dehydrogenase. The invention further provides methods of producing products by culturing the bacterium in the presence of a gaseous substrate containing one or more of CO, CO2, and H2. [Continued on next page] WO 2017/200884 Al MIDEDIMOMMIDIRMEM0010101EIHMINVOIMIE TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h))