Patent SG-11201806916R-A (SG)
Title: A method for increasing resistant starch and dietary fibre in rice
Inventor: RAJA DR R BHARATHI
IPC: A01H5/10
Filed: 2017-02-02
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/141128 Al 24 August 2017 (24.08.2017) WIPO I PCT 111111111111110111011111111111010111110111010111110111011101111101111111111111111111 W O 20 17 / 141128 Al (51) International Patent Classification: C12N 15/82 (2006.01) A23L 29/212 (2016.01) A23L 29/00 (2016.01) (21) International Application Number: PCT/IB2017/050558 (22) International Filing Date: 2 February 2017 (02.02.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 201641005181 15 February 2016 (15.02.2016) IN (71) Applicants: UDAYA AGRO FARM [IN/IN]; 62/61, Bharath Nagar, Kumbakonam 612001 (IN). GREEN FLORA BIOSCIENCES PVT. LTD [IN/IN]; Plot No. 14, Maxworth Nagar, Phase III, Kolapakkam, Chennai 600128 (IN). (72) Inventor: RAJA, Dr R. Bharathi; Number 27, San- jeevirayanpet 4th Street, Tindivanam, Villupuram District, Tamil Nadu, Tindivanam 604001 (IN). (74) Agents: KALIA, Anita et al.; #213, Sobha Aquamarine, Sarjapur Outer Ring Road, Bellandur, Bangalore - Karnataka 560103 (IN). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) = (54) Title: A METHOD FOR INCREASING RESISTANT STARCH AND DIETARY FIBRE IN RICE Sample No. and details of mutations detected % Chain distribution Amylose content % AC Resistant Total Starch % RS Dietary Fibre % TDF Hydrolysis Index % HI Short Chains (DP 6- 12) Medium Chains (DP 13- (DP>19) 18) Long Chains Lotus 1 3 (ss111 - a, sbel and sbellb 2 ) 35.75 43.93 20.32 26.8 9.37 11.12 38.4 Lotus 2 2 (ssl and sbellb 2 ) 25.49 52.06 22.45 25.1 5.12 6.87 48.2 Lotus 3 2 (shell - h i and ss///a) 31.05 46.23 22.71 27.4 7.07 9.74 42.1 Lotus 4 2 (ssl and ss 111a) 42.34 40.75 16.91 29.3 11.92 13.21 33.2 Os 278 Nil 22.19 48.96 28.86 23.4 1.21 1.87 72.6 Figure 1 (57) : The present invention discloses mutations in the starch synthase genes associated with enhanced dietary fibre and res - istant starch levels in the endosperm of a suitable variety of rice. The dietary fiber and resistant starch are enhanced to an extent to significantly reduce the hydrolysis index values of the rice grains to 35-40%. These rice varieties are in great demand for diabetic population and provide a number of other health benefits such as reduced body weight gain, cardiac health and colon health. As this strategy does not involve the use of genetic manipulation technologies, it can be directly employed in the rice breeding programmes without any restrictions.