Patent SG-11201811782V-A (SG)
Title: High resolution systems, kits, apparatus, and methods using combinatorial media strategies for high throughput microbiology applications
Inventor: HALLOCK ALEXANDER
IPC: C12M1/18
Filed: 2017-06-27
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property C.--.` 111111110111101110101011111011111011111101111110111011110111011111011110111111 Organization International Bureau (10) International Publication Number 03 (43) International Publication Date ......\"' WO 2018/005389 Al 04 January 2018 (04.01.2018) W I PO I PCT (51) International Patent Classification: (71) Applicant: GENERAL AUTOMATION LAB TECH- BOIL 3/00 (2006.01) Cl 2M 1/18 (2006.01) NOLOGIES, INC. [US/US]; 953 Indiana Street, Suite 372, C12M 1/16 (2006.01) Cl 2M 1/34 (2006.01) San Francisco, California 94107 (US). (21) International Application Number: (72) Inventors: HALLOCK, Alexander; 105 Camellia Av- PCT/US2017/039344 enue, Redwood City, California 94061 (US). ZENGLER, (22) International Filing Date: Karsten; 766 Cathy Lane, Cardiff by the Sea, California 27 June 2017 (27.06.2017) 92007 (US). CHRISTEY, Peter; 3828 26th Street, San Francisco, California 94131 (US). (25) Filing Language: English (74) Agent: CHEN, Yong; 358 5th Avenue, Ste. 1003, New (26) Publication Language: English York, New York 10001 (US). (30) Priority Data: (81) Designated States (unless otherwise indicated, for every 62/357,135 30 June 2016 (30.06.2016) US kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (63) Related by continuation (CON) or continuation-in-part CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (CIP) to earlier application: DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, US 15/135,377 (CIP) HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, Filed on 21 April 2016 (21.04.2016) KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, Title: HIGH RESOLUTION SYSTEMS, KITS, APPARATUS, AND METHODS USING COMBINATORIAL MEDIA (54) STRATEGIES FOR HIGH THROUGHPUT MICROBIOLOGY APPLICATIONS = = = • 0000 00 0p 00 0 • = = -::? g Z = ::: ::gev -'' ce W, ,4:: ll • • e • • .:2 - es ,c t:: - - it'\" e ?: - Sa c 8 1 —; g ° S ° 1:Wee , o : 4§g _ • • le0 S = _ r 00 0. 00 0. : = FIG. 1 ,-, C:r cc en (57) : A method for selecting a medium is provided. The method includes obtaining a microfabricated device including a le) plurality of microwells; loading a plurality of different media into the plurality of microwells such that each microwell of the plurality comprises a medium and the plurality of microwells comprises a plurality of different media; loading at least one cell from a sample 0 ----. into each microwell of the plurality microwells; incubating the microfabricated device at a predetermined condition for a predetermined 0 1-1 0 duration of time; comparing the contents of the plurality of microwells across the plurality of microwells; and based on the comparison, © determining at least one medium out of the plurality of different media. ei C [Continued on next page] WO 2018/005389 Al MIDEDIMOMOIDEIREEMOMIIMEMMEHOMEHOM OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))