Patent AU-2018203835-B2 (AU)
Title: Recombinant dna constructs and methods for modulating expression of a target gene
Inventor: IVASHUTA SERGEY I
IPC: C12N15/11
Filed: 2018-05-31
Granted: 2021-01-21
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) 
(19) World Intellectual Property Organization 
International Bureau 
(43) International Publication Date (10) International Publication 
Number 
7 January 2010 (07.01.2010) PCT W O 2010/002984 A1 
(51) International Patent Classification: [US/US]; 800 North Lindbergh Boulevard, St. Louis, MO 
C12N15/11 (2006.01) C12N 5/10 (2006.01) 63167 (US). ZHANG, Yuanji [CN/US]; 800 North Lind
(21) International Application Number: bergh Boulevard, St. Louis, MO 63167 (US).  
PCT/US2009/049392 (81) Designated States (unless otherwise indicated, for every 
kind of national protection available): AE, AG, AL, AM, 
(22) International Filing Date: AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, 
1July2009(01.072009) CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, 
(25) Filing Language: English DZ, EC, EE, EG, ES, Fl, GB, GD, GE, GH, GM, GT, 
HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, 
(26) Publication Language: English KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, 
(30) Priority Data: ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, 
61/077,244 1 July 2008 (01.07.2008) US NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, 
SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, 
(71) Applicant (for all designated States except US): MON- TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.  
SANTO TECHNOLOGY, LLC [US/US]; 800 North 
Lindbergh Boulevard, St. Louis, MO 63167 (US). (84) Designated States (unless otherwise indicated, for every 
kind of regional protection available): ARIPO (BW, GH, 
(72) Inventors; and GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, 
(75) Inventors/Applicants (for US only): IVASHUTA, ZW), Eurasian (AM, AZ, BY, KG, KZ, MD, RU, TJ, 
Sergey, I [RS/US]; 800 North Lindbergh Boulevard, St. TM), European (AT, BE, BG, CH, CY, CZ, DE, DK, EE, 
Louis, MO 63167 (US). WIGGINS, Barbara, E. ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, 
[Continued on nextpage] 
(54) Title: RECOMBINANT DNA CONSTRUCTS AND METHODS FOR MODULATING EXPRESSION OF A TARGET 
GENE 
(57) Abstract: This invention provides recombinant DNA con
FIGURE 1 structs and methods for manipulating expression of a target gene 
that is regulated by a small RNA, by interfering with the binding 
of the small RNA to its target gene. More specifically, this in
vention discloses recombinant DNA constructs encoding cleav
age blockers, 5-modified cleavage blockers, and translational in
hibitors useful for modulating expression of a target gene and 
methods for their use. Further disclosed are miRNA targets use
ful for designing recombinant DNA constructs including miR
e. NA-unresponsive transgenes, miRNA decoys, cleavage block
ers, 5-modified cleavage blockers, and translational inhibitors, 
B as well as methods for their use, and transgenic eukaryotic cells 
and organisms containing such constructs.  
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MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, Published: 
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ML, MR, NE, SN, TD, TG).  
- with sequence listing part ofdescription (Rule 5.2(a))