Patent PL-178478-B1 (PL)
Inventor: GRAYCAR THOMAS P
IPC: C12N15/57
Filed: 1994-10-13
Abstract: I Odmiany subtilizyny, zn am ien n e tym , ze ich sekw encja am inokwasowa nie wystepuje w przyrodzie i otrzym uje sie j a z prekursorowej subtilizyny i sek- w encja ta zawiera podstaw ienia innych reszt am inokwasowych w miejsce wiekszosci reszt am inokw asowych w pozycjach równowaznych z 76/99, 76/101, 76/103, 76/104, 76/107, 76/123, 76/99/101, 76/99/103, 76/99/104, 76/101/103, 76/101/104, 76/103/104, 76/104,107, 76/104/123, 76/107/123, 76/99/101/103, 76/99/101/104, 76/99/103/104, 76/101/103/104, 76/103/104/123, 76/104/107/123, 76/99/101/103/104, 76/99/103/104/123, 76/99/101/103/104/123, 76/103/104/126, 76/103/104/135, 76/103/104/197, 76/103/104/222, 76/103/104/260, 76/103/104/265, 76/103/104/126/265, 27/76/104/123/274,27/76/104/109/123/274, 27/76/104/123/218/274, 27/76/104/123,27/76/104/107/123, 27/76/104/109/123, 27/76/104/109/123/218/274, 27/76/104/123/197, 27/76/104/123/204, 27/76/104/123/206, 27/76/104/123/216, 27/76/104/123/218,27/76/104/123/260, 27/76/104/123/195/197, 27/76/104/123/195/218,27/76/104/123/197/218, 27/76/104/123/204/218, 27/76/104/123/206/218, 27/76/104/123/218/200,27/76/104/123/195/197/218, 76/103/104/217, 76/103/104/156, 76/103/104/166, 76/103/104/105, 76/101/103/104, 76/103/104/128, 76/103/104/210, 76/103/104/107, 76/103/104/204, 76/217 .76/103/104/156/166 8. DNA kodujace odmiany subtilizyny znam ienne tym , ze kodowana sekwencja am inokw asowa nie w ystepuje w przyrodzie i otrzym uje sie ja z prekurso- rowej subtilizyny zwierajacej podstaw ienia innych reszt am inokwasowych w miejsce wiekszosci reszt am inokw asowych w pozycjach rów nowaznych z 76/99, 76/101, 76/103, 76/104, 76/107, 76/123, 76/99/101, 76/99/103,76/99/104, 76/101/103, 76/101/104,76/103/104, 76/104,107,76/104/123, 76/107/123, 76/99/101/103, 76/99/101/104,76/99/103/104, 76/101/103/104,76/103/104/123,76/104/107/123,76/99/101/103/104,76/99/103/104/123, 76/99/101/103/104/123, 76/103/104/126, 76/103/104/135, 76/103/104/197, 76/103/104/222, 76/103/104/260, 76/103/104/265, 76/103/104/126/265, 27/76/104/123/274, 27/76/104/109/123/274, 27/76/104/123/218/274,27/76/104/123,27/76/104/107/123,27/76/104/109/123, 27/76/104/109/123/218/274, 27/76/104/123/197, 27/76/104/123/204, 27/76/104/123/206, 27/76/104/123/216, 27/76/104/123/218, 27/76/104/123/260, 27/76/104/123/195/197, 27/76/104/123/195/218,27/76/104/123/197/218, 27/76/104/123/204/218,27/76/104/123/206/218, 27/76/104/123/218/200, 27/76/104/123/195/197/218, 76/103/104/217, 76/103/104/156, 76/103/104/166, 76/103/104/105,76/101/103/104, 76/103/104/128, 76/103/104/210, 76/103/104/107, 76/103/104/204, 76/217 i 76/103/104/156/166 9 Wektory ekspresji, zn am ien n e tym , ze zaw ieraja D N A kodujace odmiany subtilizyny, przy czym kodow ana sekw encja am inokw asowa nie w ystepuje w przyrodzie i otrzymuje sie j a z prekursorow ej subtilizyny zwierajacej podstawienia innych reszt am inokw asowych w m iejsce wiekszosci reszt am inokw asowych w pozycjach równowaznych z 76/99, 76/101, 76/103, 76/104, 76/107, 76/123, 76/99/101, 76/99/103,76/99/104, 76/101/103, 76/101/104, 76/103/104, 76/104/107, 76/104/123, 76/107/123, 76/99/101/103, 76/99/101/104, 76/99/103/104, 76/101/103/104, 76/103/104/123, 76/104/107/123, 76/99/101/103/104, 76/99/103/104/123, 76/99/101/103/104/123, 76/103/104/126, 76/103/104/135, 76/103/104/197, 76/103/104/222, 76/103/104/260, 76/103/104/265, 76/103/104/126/265, 27/76/104/123/274,27/76/104/109/123/274, 27/76/104/123/218/274,27/76/104/123, 27/76/104/107/123,27/76/104/109/123,27/76/104/109/123/218/274, 27/76/104/123/197, 27/76/104/123/204, 27/76/104/123/206, 27/76/104/123/216, 27/76/104/123/218, 27/76/104/123/260, 27/76/104/123/195/197, 27/76/104/123/195/218, 27/76/104/123/197/218, 27/76/104/123/204/218,27/76/104/123/206/218, 27/76/104/123/218/200, 27/76/104/123/195/197/218, 76/103/104/217, 76/103/104/156, 76/103/104/166, 76/103/104/105, 76/101/103/104, 76/103/104/128, 76/103/104/210, 176/103/104/107, 76/103/104/204, 76/217 176/103/104/156/166 PL