Patent CA-3080274-A1 (CA)
Title: Methods of isolating t cells having antigenic specificity for a p53 cancer-specific mutation
Inventor: MALEKZADEH PARISA
IPC: A61K39/00
Filed: 2018-09-17
Abstract: CA 03080274 2020-03-24 (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 111111 1 11111111 111111 1 11 11111 1 111 11111 1 11 11111 11111111 1 11 11111 1 11 11111111111 1 111111 Organization 0----International Bureau (10) International Publication Number (43) International Publication Date ,.....0="1 WO 2019/067242 Al 04 April 2019 (04.04.2019) WIPO I PCT (51) International Patent Classification: Road, Potomac, Maryland 20854 (US). DENIGER, Drew C12N 5/0783 (2010.01) A 61K 39/00 (2006.01) C.; 10111 Crestwood Road, Kensington, Maryland 20895 C12N 5/0784 (2010.01) C07K 14/47 (2006.01) (US). A61K 35/1 7 (2015.01) (74) Agent: LAWLEY, Stephanie M.; Leydig, Voit & Mayer, (21) International Application Number: Two Prudential Plaza, Suite 4900, 180 North Stetson Av-PCT/U52018/051280 enue, Chicago, Illinois 60601 (US). (22) International Filing Date: (81) Designated States (unless otherwise indicated, for every 1'7 September 2018 (17.09.2018) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (25) Filing Language: English CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (26) Publication Language: English DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ED, EL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, (30) Priority Data: KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 62/565,464 29 September 2017 (29.09.2017) US MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (71) Applicant: THE UNITED STATES OF AMERICA, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, AS REPRESENTED BY THE SECRETARY, DE- SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, PARTMENT OF HEALTH AND HUMAN SERVICES TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. [US/US]; Office of Technology Transfer, National Insti-(84) Designated States (unless otherwise indicated, for every tutes of Health, 6011 Executive Boulevard, Suite 325, MSC kind of regional protection available): ARIPO (BW, GH, '7660, Bethesda, Maryland 20892-'7660 (US). GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (72) Inventors: MALEKZADEH, Parisa; 3 Washington Cir- UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, _ cle NW, Apartment 704, Washington, District of Colum- TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, . bia (US). ROSENBERG, Steven A.; 10104 Iron Gate EE, ES, FI, FR, GB, GR, HR, HU, EE, IS, IT, LT, LU, LV, (54) Title: METHODS OF ISOLATING T CELLS HAVING ANTIGENIC SPECEFICITY FOR A P53 CANCER-SPECIFIC MU-= TATION . 1000- -- -cr) : : = 1221 TMG-IRR . 75 . . = = . c.) = ,40 = L_ 800 - '* * :. = IMJ p53-wt-TMG . 0 _ ,. = . _ ¨ .r.) . = = '.. a) == .= = '. 1.11 p53-mut-TMG ..,-,,- 600- = * * '= .` * = 72 DMSO ,r :* o . = = * = = ! * = x 400- = * = = INZI LP-p53-wt-R175 <NI ¨ .= = 'di . .= = "*. ca. = = '= '= NM LP-p53-mut-R175H . cf) 200- + I ::. o = := . a. . ' = No Target (f) ." __ -1 cl I : _ , .= ¨ 0 i i II ,. r t .1 rim 0. WI El ,,,,.. P110 II 1! ¨ PMNIono ¨ ¨ 4141-F12 4141-Rx1 4196-TCR Mock TCR 1-1 T cell effector el ,re FIG. 1 el h v,z (57) Abstract: Disclosed are methods of isolating T cells having antigenic specificity for a mutated p53 amino acid sequence encod------ ed by a cancer-specific p53 mutation, the method comprising: inducing autologous APCs of the patient to present the mutated p53 GT ,1 amino acid sequence; co-culturing autologous T cells of the patient with the autologous APCs that present the mutated p53 amino acid 0 sequence; and selecting the autologous T cells. Also disclosed are related methods of preparing a population of cells, populations of " cells, pharmaceutical compositions, and methods of treating or preventing cancer. c [Continued on next page] CA 03080274 2020-03-24 WO 2019/067242 A1 111111 IIIIIIIIII 111111 11111 11111 II III 11111 1111111111 1111111111 11111 1111101111111111111 II 111111 MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: ¨ with international search report (Art. 21(3)) ¨ with sequence listing part of description (Rule 5.2(a))