Patent SG-11201804275T-A (SG)
Title: Microfluidic devices and kits and methods for use thereof
Inventor: BEAUMONT KRISTIN
IPC: G01N33/543
Filed: 2016-12-07
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -' Organization International Bureau irestYlid (43) International Publication Date .... ..1. .:*1 15 June 2017(15.06.2017) WIPO I PCT (10) WO International 111111111111311111111111111111111111111111111111111111111111111111111111111111111111111111 2017/100347 Publication Al Number (51) International Patent Classification: (81) Designated States (unless G01N 33/543 (2006.01) BOIL 3/00 (2006.01) kind of national protection AO, AT, AU, AZ, BA, (21) International Application Number: BZ, CA, CH, CL, CN, PCT/US2016/065426 DO, DZ, EC, EE, EG, (22) International Filing Date: HN, HR, HU, ID, IL, 7 December 2016 (07.12.2016) KW, KZ, LA, LC, LK, MG, MK, MN, MW, (25) Filing Language: English OM, PA, PE, PG, PH, (26) Publication Language: English SC, SD, SE, SG, SK, TN, TR, TT, TZ, UA, (30) Priority Data: ZW. 62/264,665 8 December 2015 (08.12.2015) US 62/333,821 9 May 2016 (09.05.2016) US (84) Designated States (unless 62/418,625 7 November 2016 (07.11.2016) US kind of regional protection GM, KE, LR, LS, MW, IN, MX, CO, ES, PL, SL, otherwise indicated, for every available): AE, AG, AL, AM, BB, BG, BH, BN, BR, BW, BY, CR, CU, CZ, DE, DJ, DK, DM, FI, GB, GD, GE, GH, GM, GT, IR, IS, JP, KE, KG, KN, KP, KR, LR, LS, LU, LY, MA, MD, ME, MY, MZ, NA, NG, NI, NO, NZ, PT, QA, RO, RS, RU, RW, SA, SM, ST, SV, SY, TH, TJ, TM, UG, US, UZ, VC, VN, ZA, ZM, otherwise indicated, for every available): ARIPO (BW, GH, MZ, NA, RW, SD, SL, ST, SZ, (71) Applicant: BERKELEY LIGHTS, INC. [US/US]; 5858 TZ, UG, ZM, ZW), Eurasian Horton Street, Suite 320, Emeryville, CA 94608 (US). TJ, TM), European (AL, (72) Inventors: BEAUMONT, Kristin, G.; 5858 Horton DK, EE, ES, FI, FR, L V, MC, MK, MT, NL, Street, Suite 320, Emeryville, CA 94608 (US). BEEM- SM, TR), OAPI (BF, ILLER, Peter, J.; 5858 Horton Street, Suite 320, Emeryville, CA 94608 (US). KURZ, Volker, L., S.; 5858 GW, KM, ML, MR, NE, GB, 13J, (AM, AZ, BY, KG, KZ, RU, AT, BE, BG, CH, CY, CZ, DE, GR, HR, HU, IE, IS, IT, LT, LU, NO, PL, PT, RO, RS, SE, SI, SK, CF, CG, CI, CM, GA, GN, GQ, SN, TD, TG). Horton Street, Suite 320, Emeryville, CA 94608 (US). Published: LAVIEU, Gregory, G.; 5858 Horton Street, Suite 320, with international search Emeryville, CA 94608 WANG, Xiaohua; 5858 Hor- (US). 21(3)) report (Art ton Street, Suite 320, Emeryville, CA 94608 (US). Agents: KUAN, Roger, C. K&L Gates LLP, 630 (74) et al.; Hansen Way, Palo Alto, CA 94304 (US). (54) Title: MICROFLUIDIC DEVICES AND KITS AND METHODS FOR USE THEREOF 4 240- Th 17 40 a ler Ca it all 58 0 1 , 16 r or 102 -45, 8 10 a -4 „„„„.„„„„„„„„„„„„ D 6 I 2 212 164 zo 168 - - — 2 dl 1 e gr y T I Ot P 141 2VB 4 4 PAodule .Module .Module Module M.-,dules Ai. 1421_104 L2u8 __ ---D FIG. 1B -- 52 501 5---- r---- 2a --542-, Or 202Th 120 6 0 222 28 208 211e 109 2 r104 2 102 1•%. .7 124 --4. 1 I 4 1 14e: 41 R fla 1 Il .4t IN it M 180 _ 17r2 ,, T \ • 9 &._ 2-r _ 4., , ' 1 4 .1/4.,,,,:::_ 10 Pover 20)C-9,_, f5-- : --/ ; I. 54 04 18 24-'ena YY Y 2' 122 a -a$, 0 MG go! ' . 9'k Z J . il k, • 4.,g \Wit214 ta'r ‘1207i '.'ar N 214 q nak-\214 214 0 z14a--' 0 1-1 FIG. 1A IN 1-1 (57) : In situ-generated microfluidic capture structures incorporating a solidified N and use, compositions and kits therefor are described. Microfluidic capture structures may fonned within the microfluidic environment, providing flexibility in assaying micro-objects FIG. 1C polymer network, methods of preparation be advantageously used for assays per - such as biological cells. Assay reagents 0 and analytes may be incorporated within the microfluidic capture structures.