Patent AU-2019400151-B2 (AU)
Title: Recombinant Escherichia coli system, construction method thereof, and usage in synthesis of α-1,2-fucosylated oligosaccharide
Inventor: XUE XIAOZHOU
IPC: C12N15/00
Filed: 2019-11-26
Granted: 2022-06-23
Abstract: i N j 9(- WJP? I IN -Ef |EP $ l 
(1019)h
'43 PdVT WO 2021/093022 A1 
2021 4 5 ) 20 (20.05.2021) WIPO T PWC0T 
(51) IFl--y : (JILIN)BIO-CHEMICAL TECHNOLOGY CO.,LTD) 
C12N 15/70 (2006.01) C12P19/02 (2006.01) [CN/CN]; +$ li -K f V * 
C12N 9/10 (2006.01) {W"&%Tt177, Jilin 130033 (CN)o 
(21) F PCT/CN2019/120873 (72) &l X:W (HUANG,Jin); +JdJLfliFT 
(22) , MF$NHE: 2019 411A 26 H (26.11.2019) MLt *i* #t& R M E H ,Beijing 102209 
(CN)o A't0,-(XUE,Xiaozhou); +PIdLAITV'F 
(25) ijiH : +7 FZ X JL- 4#9i X HRY, Beijing 102209 
(26) Qf ifF: (CN)0 o i 3-(LI, Yu); [ Q $ fl * 
(30)f : E M = Yt tt 290, Tianjin 300457 (CN) o T
201911119505.9 2019
4 11f15 H (15.11.2019) CN M K(QIN, Huimin); P t 
2 1 9 = ti ti 29, Tianjin 300457 (CN) 0 o 
(71) $f Aa : ff @J f 5 pfA q R is] q f(TONG, Yi); + M #4 9N tf r# V : 
(NUTRITION & HEALTH RESEARCH INSTITUTE, a EW 1ti 1717 Jilin 130033 (CN)o  k 
COFCO CORPORATION) [CN/CN]; +[Id I 1 (LI, Yi); + Pd n $ f i VF R *fa 
F, X Z t t 1717 Jilin 130033 (CN)o  M$I* 
102209 (CN)o A tM** tJIf R'i)(COFCO 
(54) Title: RECOMBINANT E. COLI SYSTEM, CONSTRUCTION METHOD THEREFOR, AND APPLICATION THEREOF IN 
SYNTHESIS OF a-1,2-FUCOSYLATED OLIGOSACCHARIDE 
S(54)& Z: tt{ttL H & a12-t{ t+fYY 
+ ~~ 
AA CGal-R BB 
cc 
-- OR 
DD? 
AA DONOR: GDP-FUCOSE 
BB RECEPTOR: GAL-R 
CC A-1,2-FUCOSYLTRANSFERASE + GDP-FUCOSE PYROPHOSPHORYLASE 
DD PRODUCT: A-1,2-FUCOSYLATED OLIGOSACCHARIDE 
(57) Abstract: The present invention relates to the technical field of genetic engineering, and provides a recombinant E. coli system, 
a construction method therefor, and an application thereof in the synthesis of an a-1,2-fucosylated oligosaccharide. The recombinant 
E. coli system has an a-1,2-fucosyltransferase gene and a GDP-fucose pyrophosphorylase gene. By transforming the a-1,2-fucosyl
transferase gene and the GDP-fucose pyrophosphorylase gene into E. coli, a genetically engineered strain is constructed to express an 
CA a-1,2-fucosyltransferase and a GDP-fucose pyrophosphorylase, and the expressed a-1,2-fucose pyrophosphorylase and GDP-fucose 
pyrophosphorylase are used for enzymatic synthesis of an a-1,2-fucosylated oligosaccharide.
W O 2021/093022 A1 ||||I ||||||IDI |||||I|IDI |||||||||i |||||||||||||||||||||||||||||| 
(CHEN, Bo); + ld J NA rN 'E T X J L -L * M) * 
#4 Rh X M X HM, Beijing 102209 (CN)o 3 FL 
(LI, Fan); +ld J 'El T XLK -L *t 4 
R X M E H-, Beijing 102209 (CN)0 o 2 (AN, 
Tai); + l q r T XK S t - * M 4tR 
M EX HMU, Beijing 102209 (CN)o 
(74)4tIgA:ILb 5( -Vpn - t I I ['i
-] (RUNPING& PARTNERS); +ldI JL S 
t IL J H hcN 9 Ft 515, 
Beijing 100190 (CN)o 
(81) $H5lMiR] R$fW(9S 
AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, 
BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, 
CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, 
GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, 
JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, 
LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, 
MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, 
PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, 
SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, 
US, UZ, VC, VN, ZA, ZM, ZWc 
(8 4) $ 1`"- (lj,@M Rh r)HA, V R V- ft WM( -P ) 9%- K I 
fif): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, 
NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), KXl (AM, 
AZ, BY, KG, KZ, RU, TJ, TM), XJI (AL, AT, BE, BG, 
CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, 
IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, 
RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, 
CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).  
- #t8WCtKdC(t#21(3))o 
- 'A.tI)]1)iJE('4191UJ5.2(a))o 
(57)tH P: -&J J'TYT ')Af t $a-1,2
P. AY f(L A P. m 9J T M 7 1,i§§$ 2 a-2 § fH GDP- A V4 NW 
N2 . M UT id P i -1,2-aWW&f § 2 H GDP- aMMM 2§ 29 §$ ,$ 
I f 2 li I a-,2 aW & M h fHGDP- A71W A V4 f M ( §, JF t it In (x-1,2- X P.W 
hf H GDP- VZ. V4fMhfl)T FM~hna-1,2- X 023G~