Patent AU-2013204758-B2 (AU)
Title: Humanized rodents that express heavy chains containing VL domains
Inventor: MACDONALD LYNN
IPC: C12N15/85
Filed: 2013-02-01
Granted: 2015-07-23
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date W O 2013/116609 Al 8 August 2013 (08.08.2013) W I PO I P CT (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every A01K 67/027(2006.01) C12N 9/64 (2006.01) kind of national protection available): AE, AG, AL, AM, C07K16/46 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, ) .a BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, (21) International Application Number: DO, DZ, EC, EE, EG, ES, Fl, GB, GD, GE, GH, GM, GT, PCT/US2013/024295 HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, (22) International Filing Date: KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, 1 February 2013 (01.02.2013) ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, (25) Filing Language: English RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, (26) Publication Language: English TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 61/593,463 1 February 2012 (01.02.2012) US (84) Designated States (unless otherwise indicated, for every 61/677,538 31 July 2012 (31.07.2012) US kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, (71) Applicant: REGENERON PHARMACEUTICALS, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, INC. [US/US]; 777 Old Saw Mill River Road, Tarrytown, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, NY 10591 (US). EE, ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (72) Inventors: MACDONALD, Lynn; 16 Gedney Way, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, White Plains, NY 10605 (US). GURER, Cagan; 8 Pamela TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, Lane, Valhalla, NY 10595 (US). MEAGHER, Karolina, ML, MR, NE, SN, TD, TG). A.; 14 Church Street, 2nd Floor, Tarrytown, NY 10591 Published: (US). STEVENS, Sean; 355 Berry Street, #413, San Fran- with international search report (Art. 2](3) cisco, CA 94158 (US). MURPHY, Andrew, J.; 10 New ton Court, Corton-on-hudson, NY 10520 (US). with sequence listing part of description (Rule 5.2(a)) (74) Agent: POBURSKY, Kevin, J.; Choate, Hall & Stewart LLP, Two International Place, Boston, MA 02110 (US). (54) Title: HUMANIZED RODENTS THAT EXPRESS HEAVY CHAINS CONTAINING VL DOMAINS Mouse Heavy Chain Locus VH DH JH CH Regions Intronic 3' Enh Enh Human K Light Chain Locus Proximal Arm Vic JK CK Intronic Enh Distal Arm (57) Abstract: Non-human animals, tissues, cells, and genetic material are provided that comprise a modification of an endogenous non-human heavy chain immunoglobulin sequence and that comprise an ADAM6 activity functional in a rodent (e.g., a mouse), f4 wherein the non-human animals rearrange human immunoglobulin light chain gene segments in the context of heavy chain constant regions and express immunoglobulin-like molecules comprising human immunoglobulin light chain variable domains fused to heavy chain constant domains that are cognate with human immunoglobulin light chain variable domains fused to light chain constant do mains.