Patent SG-11201803403X-A (SG)
Title: In situ-generated microfluidic isolation structures, kits and methods of use thereof
Inventor: BEAUMONT KRISTIN G
IPC: B01L3/00
Filed: 2016-11-22
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 1 June 2017 (01.06.2017) WIPOIPCT (10) International Publication Number WO 2017/091601 A1 (51) International Patent Classification: BOIL 3/00 (2006.01) (21) International Application Number: PCT/US2016/063387 (22) International Filing Date: 22 November 2016 (22.11.2016) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/258,957 23 November 2015 (23.11.2015) US 62/423,627 17 November 2016 (17.11.2016) US (71) Applicant: BERKELEY LIGHTS, INC. [US/US]; 5858 Horton Street, Suite 320, Emeryville, California 94608 (US). (72) Inventors: BEAUMONT, Kristin G.; 5858 Horton Street, Suite 320, Emeryville, California 94608 (US). DING, Nan-Linda; 1068 W. Broadway, Unit 302, Vancouver, British Columbia V6H 0A7 (CA). KURZ, Volker L.S.; 5858 Horton Street, Suite 320, Emeryville, California 94608 (US). LIONBERGER, Troy A.; 5858 Horton Street, Suite 320, Emeryville, California 94608 (US). LOWE, Randall D.; 5858 Horton Street, Suite 320, Emeryville, California 94608 (US). MALLEO, Daniele; 5858 Horton Street, Suite 320, Emeryville, California 94608 (US). MCFARLAND, Andrew W.; 5858 Horton Street, Suite 320, Emeryville, California 94608 (US). NEYILL, J. Tanner; 5858 Horton Street, Suite 320, Emeryville, California 94608 (US). WANG, Xiaohua; 5858 Horton Street, Emeryville, California 94608 (US). (74) Agents: KUAN, Roger C. et al.; K&L Gates, 630 Hansen Way, Palo Alto, California 94304 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, [Continued on next page] (54) Title: IN SITU-GENERATED MICROFLUIDIC ISOLATION STRUCTURES, KITS AND METHODS OF USE THEREOF (57) : In situ-generated micro- fluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to intro­ duce in real time, a variety of isolating structures including pens and barriers of­ fers improved methods of micro-object ma­ nipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed. WO 2017/091601 A1 LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(H)) — as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h))