Patent DE-102010054767-A1 (DE)
Title: Disconnecting, concentrating and/or cleaning (blood)plasma protein, virus or virus component, by dissolving/suspending first liquid and third liquid, and equilibrizing (blood)plasma protein, virus or virus component with further liquids
Inventor: MUELLER DIRK DR
IPC: G01N30/60
Filed: 2010-12-16
Abstract: The method comprises dissolving/suspending a first liquid and a third liquid in-contact-brought with a mixture of a first sorbent and a second sorbent for a sufficient time to bind a (blood)plasma protein, a virus or virus component to the sorbent, equilibrizing the (blood)plasma protein, the virus or virus component to the sorbent with a further liquid, and bounding the first and second sorbents in-contact-brought with the (blood)plasma protein, the virus or virus component with a second liquid and a fourth liquid for the sufficient time to lose the (blood)plasma protein. The method comprises dissolving/suspending a first liquid and a third liquid in-contact-brought with a mixture of a first sorbent and a second sorbent for a sufficient time to bind a (blood)plasma protein, a virus or virus component to the sorbent, equilibrizing the (blood)plasma protein, the virus or virus component to the sorbent with a further liquid, bounding the first and second sorbents in-contact-brought with the (blood)plasma protein, the virus or virus component with a second liquid and a fourth liquid for the sufficient time to lose the (blood)plasma protein, the virus or virus component of the first and second sorbents, and washing and/or regenerating the sorbent with the further liquid subsequent to bind/lose the (blood)plasma protein, the virus or virus component from the sorbent. The blood plasma protein or the virus component is a mixture of natural human or animal blood or an intermediate- or a finished product of the natural human or the animal blood such as a blood plasma or precipitation product containing a protein, which is obtained from the blood plasma according to a fractionation method. The first and second sorbents have a solid support material whose surface is functionalized with two receptor molecules, where the surface of the first sorbent is functionalized with pyridine-4-carboxamido-residues and 3-carboxamido propionic-residues. The surface of the second sorbent is functionalized with benzopyrrole-residue and 3-azapyrrole residue. A pH-value of the second and third liquids is present in a vicinity of an isoelectric point of the (blood)plasma protein, the virus or virus component. The pH of the first liquid is 4-6. The pH of the second liquid is 6.5-8.5 . The pH of the third liquid is 5.5-8.5, and the pH of the fourth liquid is 3-6.5. The (blood)plasma protein, the virus or virus component have the isoelectric point of 5.5-8.5 and a molecular weight of 100-500,000 Da, and are an immunoglobulin-G or a natural antibody. The first liquid has a buffer with a concentration of 2-20 mM. A weight ratio of the (blood)plasma protein, the virus or virus component to be purified in the mixture to a volume of the sorbent is 8-16 mg/ml. The second and third liquids have a buffer with the concentration of 80-120 mM. The second and third liquids are identical. The fourth liquid has a buffer with the concentration of 100-200 mM. The pH of the buffer is 4-6. The sorbent (1) is washed with the first liquid. The liquids are an acid solution having the concentration of 200-600 mM. The second sorbent is washed with the third liquid. The sorbent is regenerated by purging with a basic solution having a pH of 9. An independent claim is included for a device for disconnecting, concentrating and/or cleaning a (blood)plasma protein, a virus or virus component from a mixture.