Patent SG-11201808592P-A (SG)
Title: Method for selection of high m6p recombinant proteins
Inventor: DO HUNG V
IPC: C12N9/26
Filed: 2017-03-30
Abstract: Bioreactor) 600 605 613 (Protein Capturing System) (Chromatography System) 617 609 (Final Product Adjustment) (Chromatography System) => => => 603 (Filtration System) 607 611 (Viral Kill) (Viral Kill) FIG. 6 615 (Filtration System) (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/173059 Al 5 October 2017 (05.10.2017) WIPO I PCT 111111111111110111011111111111010111110 0101111101111111111111110111111111110111111 (51) International Patent Classification: C12N 9/26 (2006.01) (21) International Application Number: PCT/US2017/024981 (22) International Filing Date: 30 March 2017 (30.03.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/315,400 30 March 2016 (30.03.2016) US 62/457,584 10 February 2017 (10.02.2017) US 15/473,994 30 March 2017 (30.03.2017) US (71) Applicant: AMICUS THERAPEUTICS, INC. [US/US]; 1 Cedar Brook Drive, Cranbury, New Jersey 08512 (US). (72) Inventors: DO, Hung V.; 1 Cedarbrook Drive, Cranbury, New Jersey 08512 (US). GOTSCHALL, Russell; 1 Cedar Brook Drive, Cranbury, New Jersey 08512 (US). (74) Agent: ALEGRIA, Rory P.; Servilla Whitney LLC, 33 Wood Avenue South, Suite 830, Iselin, New Jersey 08830 (US). — (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a)) = (54) Title: METHOD FOR SELECTION OF HIGH M6P RECOMBINANT PROTEINS (57) : Methods for the production, capturing and purification of recombinant human lysosomal proteins are described. Such recombinant human lysosomal proteins can have high content of mannose-6-phosphate residues. Also described are pharmaceutical compositions comprising such recombinant human lysosomal proteins, as well as methods of treatment and uses of such recombin- ant human lysosomal proteins. W O 20 1717 / 305 9 Al