Patent ES-2813727-T3 (ES)
Title: Variantes de proteasa y polinucleótidos que las codifican
Inventor: NIELSEN JENS
IPC: C12N9/54
Filed: 2015-12-18
Granted: 2021-03-24
Abstract: Variante de proteasa que comprende la alteracion S27K, donde cada posicion corresponde a la SEQ ID N.o: 3, y donde la variante de proteasa tiene al menos un 90 % de identidad de secuencia con la SEQ ID N.o: 3 y comprende un conjunto de alteraciones seleccionadas del grupo que consiste en: - V2H,S4K,S27K,S173P,S175P,F180Y,Q198E,T297P; - S4K,S27K,S173P,S175P,F180Y,Q198E,T297P; - S4K,S27K,1137E,S173P,S175P,F180Y,T297P; - A1*,V2*,P3*,S4K,S27K,S173P,S175P,F180Y,Q198E,T297P; donde "*" indica una delecion; - S4K,S27K,N87K,E127Q,G132H,S173P,S175P,F180Y,Q198E,T297P; - N16K,D17N,S27K,S173P,S175P,F180Y,Q198E,T297P; - N16H,S27K,S173P,S175P,F180Y,Q198E,T297P; - N16H,S27K,I137E,S173P,S175P,F180Y,T297P; - D17N,S27K,S173P,S175P,F180Y,Q198E,T297P; - D17N,S27K,1137E,S173P,S175P,F180Y,T297P; - Q18K,S27K,E127Q,S173P,S175P,F180Y,Q198E,T297P; - S27K,S173P,S175P; - S27K,I137E,S173P,S175P,F180Y,T297P; - S27K,S173P,S175P,Q198E; - S27K,S173P,S175P,F180Y,Q198E,T297P; - S27K,S144R,S173P,S175P,F180Y,Q198E,T297P; - S27K,D147N,S173P,S175P,F180Y,Q198E,T297P; - S27K,E127Q,S173P,S175P,F180Y,Q198E,T297P; - S27K,E50Q,S173P,S175P,F180Y,Q198E,T297P; - S27K,N87K,S173P,S175P,F180Y,Q198E,T297P; - S4K,S27K,S173P,S175P,F180Y,Q198E,T297P; - S27K,S173P,S175P,F180Y,Q198E,S270K,T297P; - S27K,Q97K,S173P,S175P,F180Y,Q198E,T297P; - S27K,I137E,S144R,S173P,S175P,F180Y,T297P; - S27K,E127Q,I137E,S173P,S175P,F180Y,T297P; - S27K,I137E,S173P,G174R,S175P,F180Y,T297P; - S27K,Q97K,I137E,S173P,S175P,F180Y,T297P; - D17N,S27K,I137E,S173P,S175P,F180Y,T297P; - S27K,D126G,S173P,S175P,F180Y,T297P; - S27K,Y39R,S173P,S175P,F180Y,Q198E,T297P; - S27K,Y39R,I137E,S173P,S175P,F180Y,T297P; - S27K,Y39R,N87K,I137E,S173P,S175P,F180Y,T297P; - S27K,Y39R,I137E,S144R,S173P,S175P,F180Y,T297P; - S27K,Y39R,1121V,S171N,S173P,G174R,S175P,F180Y,Q198E,T297P; - S27K,S41K,E127Q,S173P,S175P,F180Y,Q198E,T297P; - S27K,S65K,S173P,S175P,F180Y,T297P; - S27K,N87K,I137E,S173P,S175P,F180Y,T297P; - S27K,N87K,N109S,S111R,S173P,S175P,F180Y,Q198E,T297P; - S27K,N59L,T67V,N87K,G107N,I121V,S171N,S173P,G174R,S175P,F180Y,Q198E, T297P; - S27K,N59L,T67V,N87K,G107N,I121V,E127Q,S171N,S173P,G174R,S175P,F180Y, Q198E,T297P; - S27K,Q70N,N87K,I121V,E127Q,S171N,S173P,G174R,S175P,F180Y,Q198E,N199R, T297P; - S27K,N59L,T67V,Q97K,G107N,I121V,S171N,S173P,G174R,S175P,F180Y,Q198E, T297P; - S27K,N109K,S173P,S175P,F180Y,Q198E,T297P; - S27K,N109S,S111R,S173P,S175P,F180Y,Q198E,T297P; - S27K,S111R,A118R,S173P,S175P,F180Y,Q198E,T297P; - S27K,D126G,S173P,S175P,F180Y,Q198E,T297P; - S27K,D126G,E127Q,S173P,S175P,F180Y,Q198E,T297P; - S27K,E127Q,I137Q,S173P,S175P,F180Y,Q198E,T297P; - S27K,E127Q,S144R,S173P,S175P,F180Y,Q198E,T297P; - S27K,E127Q,V162S,S173P,S175P,F180Y,Q198E,T297P; - S27K,E127Q,V162E,S173P,S175P,F180Y,Q198E,T297P; - S27K,E127Q,V162Q,S173P,S175P,F180Y,Q198E,T297P; - S27K,E127Q,V162G,S173P,S175P,F180Y,Q198E,T297P; - S27K,E127Q,V162F,S173P,S175P,F180Y,Q198E,T297P; - S27K,E127Q,V162T,S173P,S175P,F180Y,Q198E,T297P; - S27K,E127Q,V162Y,S173P,S175P,F180Y,Q198E,T297P; - S27K,N109S,S111R,E127Q,S173P,S175P,F180Y,Q198E,T297P; - S27K,N59L,T67V,N87K,G107N,I121V,E127Q,S171N,S173P,G174R,S175P,F180Y, Q198E, T297P; - S27K,N87K,I121V,E127Q,S171N,S173P,S175P,F180Y,T297P; - S27K,N109S,S111R,D147N,S173P,S175P,F180Y,Q198E,T297P; - S27K,K146E,D147S,S171N,S173P,G174R,S175P,F180Y,G182A,Q198E,N199K, E223K,T297P; - S4K,D17N,S27K,N59L,N87K,Y156R,S173P,S175P,F180Y,Q198E,T297P; - S27K,S173P,G174R,S175P,F180Y,Q198E,T297P; - S27K,N59L,T67V,N87K,S114V,I121V,I150L,S171N,S173P,G174R,S175P,F180Y, T297P; - S27K,E127Q,S173P,S175P,F180Y,5 E194S,Q198E,T297P; - S27K,E127Q,S173P,S175P,F180Y,E194K,Q198E,T297P; - S27K,E127Q,S173P,S175P,F180Y,E194Q,Q198E,T297P; - S27K,E127Q,S173P,S175P,F180Y,N195H,V196N,Q197*,Q198*,N199*,G200*,T201*, T297P; donde "*" indica una delecion; - S27K,S173P,S175P,F180Y,Q197K,T297P; - S27K,S173P,S175P,F180Y,E194R,Q197K,Q198E,T297P; - S27K,S173P,S175P,F180Y,Q197K,Q198E,T297P; - S27K,E127Q,S173P,S175P,F180Y,Q197K,Q198E,T297P; - S27K,S171N,S173P,G174R,S175P,F180Y,Q197K,Q198E,T297P; - S27K,N109S,S111R,S173P,S175P,F180Y,Q197K,Q198E,T297P; - S27K,S173P,G174K,S175P,F180Y,L184F,Q197K,Q198E,T297P; - S27K,S171N,S173P,G174R,S175P,F180Y,Q197R,Q198E,T297P; - S27K,S173P,S175P,F180Y,E194*,Q197K,Q198E,N199K,T297P; donde "*" indica una delecion; - S27K,E127Q,S173P,S175P,F180Y,Q198E,N199K,T297P; - S27K,I121V,S171N,S173P,G174R,S175P,F180Y,Q198E,N199K,T297P; - S27K,S171N,S173P,G174R,S175P,F180Y,Q198E,N199K,T297P; - S27K,N109S,S111R,S173P,S175P,F180Y,Q198E,N199K,T297P; - S27K,S173P,S175P,F180Y,Q197K,Q198E,N199K,T297P; - S27K,Q70N,S171N,S173P,G174R,S175P,F180Y,Q198E,N199K,T297P; - S27K,I150S,S171N,S173P,G174R,S175P,F180Y,Q198E,N199K,T297P; - S27K,S114V,S171N,S173P,G174R,S175P,F180Y,Q198E,N199K,T297P; - S27K,Q70N,G107N,I121V,E127Q,S173P,S175P,F180*Y,Q198E,N199K,T297P; - S27K,Q70A,S171N,S173P,G174R,S175P,F180Y,Q198E,N199K,T297P; - S27K,Q70V,S171N,S173P,G174R,S175P,F180Y,Q198E,N199K,T297P; - S27K,S173P,G174K,S175P,F180Y,L184F,Q198E,N199K,T297P; - S27K,S171D,S173P,G174R,S175P,F180Y,Q198E,N199K,T297P; - S27K,S171R,S173P,G174E,S175P,F180Y,Q198E,N199K,T297P; - S27K,S171K,S173P,G174E,S175P,F180Y,Q198E,N199K,T297P; - S27K,S171E,S173P,G174K,S175P,F180Y,Q198E,N199K,T297P; - S27K,Q97K,E127Q,S173P,S175P,F180Y,Q198E,D225N,I247M,T297P; - T5C,S27K,S171N,S173P,G174R,S175P,F180Y,Q198E,E236C,T297P; - Q6C,S27K,S171N,S173P,G174R,S175P,F180Y,Q198E,E236C,T297P; - S4C,S27K,S171N,S173P,G174R,S175P,F180Y,Q198E,E236C,T297P; - A1*,T5C,S27K,S171N,S173P,G174R,S175P,F180Y,Q198E,E236C,T297P; donde "*" indica una delecion; - A1*,Q6C,S27K,S171N,S173P,G174R,S175P,F180Y,Q198E,E236C,T297P; donde "*" indica una delecion; - AT*,S4C,S27K,S171N,S173P,G174R,S175P,F180Y,Q198E,E236C,T297P; donde "*" indica una delecion; - A1*,P3*,T5C,S27K,S171N,S173P,G174R,S175P,F180Y,Q198E,E236C,T297P; donde "*" indica una delecion; - S27K,E127Q,S173P,S175P,F180Y,Q198E,N268K,T297P; - S27K,I137E,S173P,S175P,F180Y,S270K,T297P; - S27K,N87K,I121V,S171N,S173P,S175P,F180Y,S270K,T297P; - S27K,S173P,S175P,F180Y,S270K,T297P; - S27K,S173P,S175P,F180Y,Q198E,I293R,T297P; - S27K,I137E,S173P,S175P,F180Y,I293R,T297P; - S27K,D147N,I150S,S171D,S173P,G174R,S175P,F180Y,G182A,L184F,Q197K,Q198E, S274V,T297P; - S27K,D147N,I150S,S171D,S173P,G174R,S175P,F180Y,G182A,L184F,Q198E,S274V, T297P; - S27K,D147N,I150S,S171N,S173P,G174R,S175P,F180Y,Q198E,N199K,R224G,T297P; - S27K,D147S,I150S,S171D,S173P,G174R,S175P,F180Y,G182A,L184F,Q198E,S274V, T297P; - S27K,G142E,D147N,I150S,S171N,S173P,G174R,S175P,F180Y,G182A,L184F,Q198E, N199K,T297P; - S27K,I150L,S171N,S173P,G174R,S175P,F180Y,Q198E,N199K,T297P; - S27K,I150S,S171D,S173P,G174R,S175P,F180Y,G182A,L184F,Q198E,N199K,S274V, T297P; - S27K,N109K,S111D,S171D,S173P,G174R,S175P,F180Y,Q198E,T297P; - S27K,N109K,S111D,S171E,S173P,G174K,S175P,F180Y,G182A,L184F,Q198E,N199K, T297P; - S27K,N109K,S111E,S171 D,S173P,G174R,S175P,F180Y,Q198E,T297P; - S27K,N109K,S111E,S171 E,S173P,G174K,S175P,F180Y,G182A,L184F,Q198E,N199K,T297P; - S27K,N109K,S171D,S173P,G174R,S175P,F180Y,Q198E,T297P; - S27K,N109S,*109aE,S111R,S171E,S173P,G174K,S175P,F180Y,G182A,L184F,Q198E,N199K,T297P; donde "*109aE" indica una insercion; - S27K,N109S,I121V,S171D,S173P,G174R,S175P,F180Y,G182A,L184F,Q198E,N199K, S274V,T297P; - S27K,N87K,G132H,V162T,S171D,S173P,G174R,S175P,F180Y,G182A,L184F,Q198E,S274V,T297P; - S27K,N87K,S171D,S173P,G174R,S175P,F180Y,G182A,L184F,Q198E,T297P; - S27K,N87K,S171D,S173P,G174R,S175P,F180Y,Q198E,T297P; - S27K,N87K,S173P,G174K,S175P,F180Y,G182A,L184F,Q198E,V286R,T297P; - S27K,N87K,V162T,S171D,S173P,G174R,S175P,F180Y,G182A,L184F,Q198E,S274V,T297P; - S27K,Q70A,D147N,S171D,S173P,G174R,S175P,F180Y,G182A,L184F,Q198E,N199K,S274V,T297P; - S27K,Q70A,N109K,S111E,S171D,S173P,G174R,S175P,F180Y,G182A,L184F,Q198E,N199K,S274V,T297 P; - S27K,Q70A,S171D,S173P,G174R,S175P,F180Y,G182A,L184F,Q198E,N199K,S274V,T297P; - S27K,S111R,S171D,S173P,G174R,S175P,F180Y,G182A,L184F,Q198E,S270V,S274V,L280V,T297P; - S27K,S114V,I121V,S129K,S173P,G174K,S175P,F180Y,L184F,Q198E,N199K,T297P; - S27K,S171D,S173P,G174R,S175P,F180Y,Q197K,Q198E,T297P; and - S4K,S27K,N87K,E127Q,G132H,Y156R,S173P,S175P,F180Y,Q198E,T297P.