Patent SG-11201810179R-A (SG)
Title: Novel crispr enzymes and systems
Inventor: ZHANG FENG
IPC: C12N9/22
Filed: 2017-04-19
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1#1110111101110101011111010111110111011111101110111111011101111011110111111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\" WO 2017/184768 Al 26 October 2017 (26.10.2017) WIPO I PCT (51) International Patent Classification: (US). COX, David Benjamin, Turitz [US/US]; 950 Mass- C12N 9/22 (2006.01) achusetts Ave., Apt. 217, Cambridge, MA 02139 (US). (21) International Application Number: (74) Agent: KOWALSKI, Thomas, J. et al.; Vedder Price P.C., PCT/US2017/028420 1633 Broadway, New York, NY 10019 (US). (22) International Filing Date: (81) Designated States (unless otherwise indicated, for every 19 April 2017 (19.04.2017) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (25) Filing Language: English CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (26) Publication Language: English DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, (30) Priority Data: KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, 62/324,820 19 April 2016 (19.04.2016) US MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, 62/351,558 17 June 2016 (17.06.2016) US PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, 62/360,765 11 July 2016 (11.07.2016) US SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, 62/410,196 19 October 2016 (19.10.2016) US TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (71) Applicants: THE BROAD INSTITUTE INC. [US/US]; (84) Designated States (unless otherwise indicated, for every 415 Main Street, Cambridge, MA 02142 (US). kind of regional protection available): ARIPO (BW, GH, MASSACHUSETTS INSTITUTE OF TECHNOLOGY G [US/US]; 77 Massachusetts Ave., Cambridge, MA 02139 M, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, _ (US). UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, = (72) Inventors; and EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (71) Applicants: ZHANG, Feng [US/US]; 100 Pacific Street, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, = Apt. 11, Cambridge, MA 02139 (US). ZETSCHE, Bernd TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, — [DE/US]; 383 Washington Street, Gloucester, MA 01930 KM, ML, MR, NE, SN, TD, TG). = _ (US). GAO, Linyi [US/US]; 70 Pacific Street, #482C, Cam- = bridge, MA 02139 (US). SLAYMAKER, Ian [US/US]; 1783 Massachusetts Ave., Apt. 1, Cambridge, MA 02140 = = (54) Title: NOVEL CRISPR ENZYMES AND = = = _ = = = = = = ,-1 Ot P V::+ A l Ot 1-1 - -- t .., (57) : The invention provides for systems, 1 -1 provides non-naturally occurring or engineered 0 and at least one targeting nucleic acid component ei altered PAM specificity. ... SYSTEMS m v like a methods, DNA-targeting systems comprising FIG. guide , 4 and RNA. SM2R1OOTR 20D ..4 ... compositions for targeting Aspects I T of the SM2F4,10443VMSVg a invention 4 . novel DNA -targeting CRISPR effector protein nucleic in particular acids. In particular, the invention relate to Cpfl mutants having [Continued on next page] WO 2017/184768 Al MIDEDIM011111 11111111MEM31111111111111111111110111101111111111111111111 Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event amendments (Rule 48.2(h)) of receipt of