Patent SG-11201804563S-A (SG)
Title: Digital microfluidic system for single-cell isolation and characterization of analytes
Inventor: JAMSHIDI ARASH
IPC: G01N15/10
Filed: 2016-11-30
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau IIIIII Hit II EH DID IIIII DEEM II 11111111111111111111111111111111R1111111 (54) Title: DIGITAL MICROFLUIDIC SYSTEM FOR SINGLE-CELL ISOLATION AND CHARACTERIZATION OF ANA- LYTES 220 Figure 5B 115 (57) : In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microw- ell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads. (10) International Publication Number WO 2017/095917 Al WIPO I PCT (43) International Publication Date 8 June 2017 (08.06.2017) (74) Agent: SMALL, Dean, D.; The Small Patent Law Group LLC, 225 S. Meramec, Suite 725, St. Louis, MO 63105 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art 21(3)) (51) International Patent Classification: C12Q 1/68 (2006.01) BOIL 3/00 (2006.01) G01N 1/28 (2006.01) GO1N 15/10 (2006.01) (21) International Application Number: PCT/US2016/064206 (22) International Filing Date: 30 November 2016 (30.11.2016) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/261,786 62/281,510 62/314,071 62/358,968 US US US US 1 December 2015 (01.12.2015) 21 January 2016 (21.01.2016) 28 March 2016 (28.03.2016) 6 July 2016 (06.07.2016) (71) Applicant: ILLUMINA, INC. [US/US]; 5200 Illumina, Way, San Diego, CA 92122 (US). (72) Inventors: JAMSHIDI, Arash; 499 Illinois Street, Suite 210, San Francisco, CA 94158 (US). LIN, Yan-you; 499 Illinois Street, Suite 210, San Francisco, CA 94158 (US). ABSALAN, Farnaz; 499 Illinois Street, Suite 210, San Francisco, CA 94158 (US). STUART, Sarah; 499 Illinois Street, Suite 210, San Francisco, CA 94158 (US). CANN, Gordon; 499 Illinois Street, Suite 210, San Francisco, CA 94158 (US). WU, Yir-Shyuan; 499 Illinois Street, Suite 210, San Francisco, CA 94158 (US). KHURANA, Tarun; 499 Illinois Street, Suite 210, San Francisco, CA 94158 (US). FISHER, Jeffrey, S.; 499 Illinois Street, Suite 210, San Francisco, CA 94158 (US). W O 20 17 /095 9 17 Al