Patent SG-11201805049T-A (SG)
Title: Pellets used in cell culture and methods of making thereof
Inventor: PHELPS MWITA
IPC: C12N5/00
Filed: 2016-12-16
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization M1111101110101011111 HO 11111 01110101101100111 11111 HEIM III 11 International Bureau 0.. .1 j ..... ..or::,„,' (10) International Publication Number (43) International Publication Date WO 2017/106783 Al 22 June 2017(22.06.2017) WIPO I PCT (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12N 5/00 (2006.01) Cl 2N 1/22 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (21) International Application Number: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, PCT/US2016/067374 DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (22) International Filing Date: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, 16 December 2016 (16.12.2016) KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, (25) Filing Language: English NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (26) Publication Language: English RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, (30) Priority Data: ZA, ZM, ZW. 62/269,031 17 December 2015 (17.12.2015) US (84) Designated States (unless otherwise indicated, for every (71) Applicant: LIFE TECHNOLOGIES CORPORATION kind of regional protection available): ARIPO (BW, GH, [US/US]; 5823 Newton Drive, Carlsbad, California 92008 GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, (US). TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (72) Inventors: PHELPS, Mwita; 5823 Newton Drive, Carls- TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, bad, California 92008 (US). GULDE, Paul; 5823 Newton LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, Drive, Carlsbad, California 92008 (US). FIKE, Richard; SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, 9310 Hunting Valley North, Clarence, New York 14031 (US). REYNOLDS, Mary; 5823 Newton Drive, Carlsbad, GW, KM, ML, MR, NE, SN, TD, TG). California 92008 (US). HASSETT, Richard; 5823 New- Declarations under Rule 4.17: ton Drive, Carlsbad, California 92008 (US). CAMPBELL, — of inventorship (Rule 4.17(iv)) Andrew; 5823 Newton Drive, Carlsbad, California 92008 (US). Published: = (74) Agents: REDDY, Daphne et al.; Life Technologies Cor- — with international search report (Art. 21(3)) poration, 5823 Newton Drive, Carlsbad, California 92008 (US). = (54) Title: PELLETS USED IN CELL CULTURE AND METHODS OF MAKING THEREOF FIG. 8: Pelletizing Process Dispense formulation into Liquid and Powder groups Tumble blend groups to non homogeneous mixture (-5min) Liquid Formulation of Liquid groups Particle size reduction via Fitzmill continuous mill Sterile Filtration of Liquid groups Il Tumble blend milled powder to homogeneous (-20min) G GC Rotor Fluidized Bed Processing for Ir----- pellet formation I-1 (57) : The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstit - ---- uteri into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for ex - Ir --- ample, different vitamins, amino acids, buffers, trace salts, pH, iron chelators, etc. The invention also relates to methods of making 1-1 C dry cell culture media by altering ratios of different pellet compositions, or, methods of making modular dry cell culture media, or ei customizing media formulations for growing a cell type using pellets. According to the invention, media pellets may be easier to en. ) handle either before reconstitution, during shipping and handling; and/or during reconstitution. Media pellets may be used in any container like bags including sterile, single use bags for preparing media formulations. The invention also relates to kits and culture systems using media pellets.