Patent SG-11201808740T-A (SG)
Title: Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor
Inventor: MARRAS SALVATORE A E
IPC: C12Q1/68
Filed: 2017-04-05
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 111111110111101110101011111010111110111010111111011111111101011111101111011111 International Bureau 0.. .... .. (10) International Publication Number (43) International Publication Date ...... ../ WO 2017/176852 Al 12 October 2017(12.10.2017) WIPO I PCT (51) International Patent Classification: (74) C12Q 1/68 (2006.01) Cl 2P 19/30 (2006.01) Agents: NORTON, Gerard P. et al.; Fox Rothschild LLP, 997 Lenox Drive, Building 3, Lawrenceville, NJ 08648- C12Q 1/08 (2006.01) 2311 (US). (21) International Application Number: (81) PCT/US2017/026088 Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (22) International Filing Date: 5 April 2017 (05.04.2017) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (25) Filing Language: English HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, (26) Publication Language: English MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, (30) Priority Data: 62/319,332 7 April 2016 (07.04.2016) US NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, (71) Applicant: RUTGERS, THE STATE UNIVERSITY OF ZA, ZM, ZW. NEW JERSEY [US/US]; 83 Somerset Street, New Brun- swick, NJ 08901 (US). (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (72) Inventors: MARRAS, Salvatore A.E.; 104 West Roselle GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, Avenue, Roselle Park, NJ 07204 (US). VARGAS-GOLD, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, Diana; 119 Millburn Avenue, Millburn, NJ 07041 (US). TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, TYAGI, Sanjay; 99 John Street, Apt 2109, New York, NY DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, = 10038 (US). KRAMER, Fred Russell; 561 West 231 LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, Street, Riverdale, NY 10463 (US). = [Continued on next page] (54) Title: MULTIPLEX NUCLEIC ACID ASSAY METHODS CAPABLE OF DETECTING CLOSELY RELATED ALLELES, = = AND REAGENTS THEREFOR (57) : This invention discloses multi-part = FIGURE 1 163 1J7 105 primers for primer-dependent nucleic acid amplification methods. Also disclosed are multiplex assay methods, related reagent kits, and oligonucleotides for such meth- ods. = — = - - _ = kY 106 103 1'1 = 110 = 210 211 IF , = 212- = 105 213 ' 4. 4 104 106 r --2D1 ......,...,107 = 204.,i 1 = 2 205 03 11 7 ei 105 104 106 kin 210 215 GC 211 2 45 11 /6 IN 11 ei O WO 2017/176852 Al MIDEDIM01101DERDEEMOM01011110111111111111101111111011110111111 SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, Published: GW, KM, ML, MR, NE, SN, TD, TG). — with international search report (Art. 21(3))