Patent SG-11201804894X-A (SG)
Title: Improved recombinant production method
Inventor: KILLIAN TOBIAS
IPC: C12P21/02
Filed: 2016-12-15
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 111111110111101110101011111 HO 11111 0111010110101111110111111101111101111 MI International Bureau .. .... ..Yejd .... ...,,,' (10) International Publication Number (43) International Publication Date WO 2017/102908 Al 22 June 2017(22.06.2017) WIPO I PCT (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12P 21/02 (2006.01) C12N 5/00 (2006.01) kind of national protection available): AE, AG, AL, AM, C12N 1/20 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (21) International Application Number: DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, PCT/EP2016/081120 HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, (22) International Filing Date: KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, 15 December 2016 (15.12.2016) MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (25) Filing Language: English RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, (26) Publication Language: English TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 15200528.6 16 December 2015 (16.12.2015) EP (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (71) Applicant (for all designated States except US): F. HOFF- GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, MANN-LA ROCHE AG [CH/CH]; Grenzacherstrasse TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, 124, 4070 Basel (CH). TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, (71) Applicant (for US only): HOFFMANN-LA ROCHE DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, INC. [US/US]; Great Notch, 150 Clove Road, 8th Floor, Little Falls, New Jersey 07424 (US). SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (72) Inventors: KILLIAN, Tobias; WalserstraBe 6A, 82377 Published: Penzberg (DE). NEUBAUER, Markus; Vogelbergstrasse 12, 82377 Penzberg (DE). — with international search report (Art. 21(3)) (74) Agents: SKOLAUT, Alexander et al.; Roche Diagnostics GmbH, Patent Department (LPP 6164), P.O.Box 11 52, 82372 Penzberg (DE). (54) Title: IMPROVED RECOMBINANT PRODUCTION METHOD A: Figure 3 — B: as 1 I 1 ,.. I /no eo 1-1 GC 1 © I • ' LS tz. ei 1 2 3 4 5 6 7 1 2 3 4 5 6 0 1-1 IN 1-1 (57) : Herein is reported a method for the recombinant production of a polypeptide in a eukaryotic cell comprising the steps 0 of (i) cultivating a eukaryotic cell comprising a nucleic acid encoding the polypeptide in a cultivation medium comprising a com - N pound selected from the group consisting of trans-2-methyl 2-pentenoic acid, the broad-spectrum HDAC inhibitor Quisinostat, and C the subtype-specific HDAC inhibitor Romidepsin, and (ii) recovering the polypeptide from the cell or the cultivation medium and thereby producing the polypeptide in a eukaryotic cell.