Patent SG-11201901959Y-A (SG)
Title: Modified stem cell memory t cells, methods of making and methods of using same
Inventor: OSTERTAG ERIC
IPC: C12N5/0783
Filed: 2017-10-02
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 11111111111111111111111111111111111111111111111111111111111101111111011111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\" WO 2018/064681 Al 05 April 2018 (05.04.2018) WIP0 I PCT (51) International Patent Classification: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, C12N 5/0783 (2010.01) A61K 35/17(2015.01) TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (21) International Application Number: (84) Designated States (unless otherwise indicated, for every PCT/US2017/054799 kind of regional protection available): ARIPO (BW, GH, (22) International Filing Date: GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, 02 October 2017 (02.10.2017) UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, (25) Filing Language: English EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (26) Publication Language: English MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, (30) Priority Data: KM, ML, MR, NE, SN, TD, TG). 62/402,707 30 September 2016 (30.09.2016) US 62/502,508 05 May 2017 (05.05.2017) US Published: 62/553,058 31 August 2017 (31.08.2017) US — with international search report (Art. 21(3)) 62/556,309 08 September 2017 (08.09.2017) US — with sequence listing part of description (Rule 5.2(a)) (71) Applicant: POSEIDA THERAPEUTICS, INC. [US/US]; 4242 Campus Point Court, Suite 700, San Diego, California 92121 (US). (72) Inventors: OSTERTAG, Eric; 4242 Campus Point Court, Suite 700, San Diego, California 92121 (US). SHED- _ LOCK, Devon; 4242 Campus Point Court, Suite 700, San = Diego, California 92121 (US). = Agent: ELRIFI, Ivor R. et al.; Cooley LLP, 1299 Pennsyl- (74) vania — Avenue, NW, Suite 700, Washington, District of Co- lumbia 20004 (US). Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (81) = = = DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, = = MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, — _ = Title: MODIFIED STEM CELL MEMORY T CELLS, METHODS OF MAKING AND METHODS OF USING SAME = (54) = = FIG. 18A FIG. 18B _ = ›, >, . ,.. ;Ts, 0 t „ g E. plAIVIEJ 2 -?! prAMEJ ,7, pHR only pHR + Cas9 RNP g pHR + Cas9 RNP pHR only + Cas9 RNP 2 2 + Cas9 RNP 2 2 V) — 1-1 1-1 Z.. = C$ DI 0 rs) = 0 0 ry) a. c' co 0 , ca la) = 01 c , to tf) E: m . 3 7, z3 3 3 q 3 3 fa. 3 — 3. 3 3 3 = 3 3 3 = 3 3 a. GC wi, cl 0 rt , m _ g 4n 0 .bc c! ' 0 . 9 tn o wt 9 ,-... xn ,.., -. (NI lin .r. , .e.. , le.. , , e- aN in , , r , Cs4 in Q . ''' 'R g .3E 9 ' t) __ 9 9. v•-• N FtS 14 T- +r- La trip V; V; MO ifff0 /1\" , © , (3-13 infr.7 , 9 ,, 11 © ei ,,..., (57) : The disclosure provides a method of producing modified stem memory T cells (e.g. CAR-T cells) for administration 5 to a subject as, for example an adoptive cell therapy.