Patent SG-11201810471Y-A (SG)
Title: T cell expansion
Inventor: WANG PETER
IPC: C12N5/0783
Filed: 2016-10-25
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1111111111111111111 111111111111111111111111111111111111111111111111111111111111111110111111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\"\" WO 2017/202478 Al 30 November 2017 (30.11.2017) WIPO I PCT (51) International Patent Classification: TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, C12N 5/0783 (2010.01) KM, ML, MR, NE, SN, TD, TG). (21) International Application Number: PCT/EP2016/075644 Published: — with international search report (Art. 21(3)) (22) International Filing Date: 25 October 2016 (25.10.2016) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/340,605 24 May 2016 (24.05.2016) US 15/290,230 11 October 2016 (11.10.2016) US (71) Applicant: TESSA THERAPEUTICS PTE LTD [SG/SG]; 46 Kim Yam Road, #01-14 The Herencia, Singa- pore 239351 (SG). (71) Applicant (for LS only): CLEGG, Richard Ian [GB/GB]; City Tower, 40 Basinghall Street, London, Greater London EC2V 5DE (GB). (72) Inventors: WANG, Peter; c/o Tessa Therapeutics Pte = Ltd, 46 Kim Yam Road, #01-14, The Herencia, Singapore = 239351 (SG). WU, Chunxiao; c/o Tessa Therapeutics Pte _ Ltd, 46 Kim Yam Road, #01-14, The Herencia, Singapore 239351 = (SG). (74) Agent: CLEGG, Richard et al.; Mewburn Ellis LLP, = City Tower, 40 Basinghall Street, London, Greater London = EC2V 5DE (GB). _ = (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, = AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, = = CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, = = DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, = KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, = — MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, _ = PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, = SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, = TZ, ZA, ZM, ZW. UA, UG, US, UZ, VC, VN, = (84) Designated States (unless otherwise indicated, for every = kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, _ TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, 1-1 EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, GC IN I I - (54) Title: T CELL EXPANSION ei 0 (57) : A method for generating or expanding a population of T cells specific for a virus by a method comprising: stimulating T ei ----. cells by culture in the presence of antigen presenting cells (APCs) presenting a peptide of the virus, wherein at least 10% of the media in IN which the cells are cultured is conditioned media obtained from a stimulation culture comprising T cells and APCs presenting a peptide © of the virus. Also disclosed are methods for accelerating the rate of expansion of a virus-specific T cell population, and methods for N treating or preventing diseases or disorders using the generated or expanded T cell population. O