Patent SG-11201902945S-A (SG)
Title: Compositions and methods for protein expression and delivery
Inventor: DRUM CHESTER LEE
IPC: A61K9/51
Filed: 2017-10-06
Abstract: NO N O GC O r•i C () INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 12 April 2018 (12.04.2018) WIP0 I PCT olimion °nolo III oiloo moninno oimIE (10) International Publication Number WO 2018/067075 Al (51) International Patent Classification: C07K 14/47 (2006.01) A61K 9/51 (2006.01) (21) International Application Number: PCT/SG2017/050505 (22) International Filing Date: 06 October 2017 (06.10.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/405,613 07 October 2016 (07.10.2016) US (71) Applicant: NATIONAL UNIVERSITY OF SINGA- PORE [SG/SG]; 21 Lower Kent Ridge Road, Singapore 119077 (SG). (72) Inventors: DRUM, Chester Lee; c/o National Universi- ty of Singapore, Yong Loo Lin School of Medicine / De- partment of Medicine, 21 Lower Kent Ridge Road, Sin- gapore 119077 (SG). MASURKAR, Nihar; c/o National University of Singapore, Yong Loo Lin School of Medi- cine / Department of Medicine, 21 Lower Kent Ridge Road, Singapore 119077 (SG). DESHPANDE, Siddharth; c/o National University of Singapore, NUS Graduate School for Integrative Science and Engineering, 21 Lower Kent Ridge Road, Singapore 119077 (SG). CHAKRABORTY, Goutam; c/o National University of Singapore, Yong Loo Lin School of Medicine / Department of Medicine, 21 Low- er Kent Ridge Road, Singapore 119077 (SG). VALLERIN- TEAVIDE MAVELLI, Girish; c/o National University of Singapore, Yong Loo Lin School of Medicine / Department of Medicine, 21 Lower Kent Ridge Road, Singapore 119077 (SG). (74) Agent: MATTEUCCI, Gianfranco; Marks & Clerk Sin- gapore LLP, Tanjong Pagar, P 0 Box 636, Singapore 910816 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) (54) Title: COMPOSITIONS AND METHODS FOR PROTEIN EXPRESSION AND DELIVERY FIG. 1A 12nm 8nm (57) : The present invention relates to methods and compositions that provide an environment that promotes enhanced protein expressing and/or folding. More particularly, the present invention provides engineered thermostable ferritin assemblies comprising at least one modified ferritin subunit, wherein the least at least one modified ferritin subunit lacks an unstructured carboxy-terminal sequence that is present in a wildtype ferritin subunit and wherein the engineered assembly is stable at lower salt concentrations that a wildtype ferritin assembly. The invention is exemplified by a ferritin assembly derived from hyperthermophilic Archaeoglobus fulgidus. Also claimed are nucleic acids, compositions, kits and methods of encapsulating a polypeptide, delivering and administering the said engineered ferritin assembly to a subject. [Continued on next page] WO 2018/067075 Al MIDEDIMOMMIDIREIEHMOHNEDIRMOUVON Published: — with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a))