Patent SG-11201810639R-A (SG)
Title: Tagmentation using immobilized transposomes with linkers
Inventor: DESANTIS GRACE
IPC: C12Q1/6806
Filed: 2018-02-20
Abstract: G Extend (DNA elutes from bead) ' 5' 5' 3' B15' Al 4 B15 A14' (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 30 August 2018 (30.08.2018) WIP0 I PCT 01E1010 llmonolomollium icioluom o (10) International Publication Number WO 2018/156519 Al (51) International Patent Classification: C12Q 1/6806 (2018.01) C12Q 1/6869 (2018.01) (21) International Application Number: PCT/US2018/018824 (22) International Filing Date: 20 February 2018 (20.02.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/461,620 21 February 2017 (21.02.2017) US (71) Applicants: ILLUMINA INC. [US/US]; 5200 Illumina Way, San Diego, CA 92122 (US). ILLUMINA CAM- BRIDGE LIMITED [GB/GB]; Chesterford Research Park, Little, Chesterford, Nr. Saffron Walden, Essex CB10 1XL (GB). (72) Inventors: DESANTIS, Grace; c/o Illumina Inc., 5200 Il- lumina Way, San Diego, CA 92122 (US). GROSS, Stephen M.; c/o Illumina Inc., 5200 Illumina Way, San Diego, CA 92122 (US). LI, Jian-Sen; c/o Illumina Inc., 5200 Illumi- na Way, San Diego, CA 92122 (US). MORRELL, Natal- ie; c/o Illumina Cambridge Limited, Chesterford Research Park, Little, Chesterford, Nr. Saffron Walden, Essex CB10 1XL (GB). SLATTER, Andrew; c/o Illumina Cambridge Limited, Chesterford Research Park, Little, Chesterford, Nr. Saffron Walden, Essex CB10 1XL (GB). SHEN, Kevin; c/ o Illumina Inc., 5200 Illumina Way, San Diego, CA 92122 (US). SNOW, Samantha; c/o Illumina Inc., 5200 Illumina Way, San Diego, CA 92122 (US). (74) Agent: FULLER, Michael L.; Knobbe, Martins Olson & Bear LLP, 2040 Main Street, Fourteenth Floor, Irvine, CA 92614 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (54) Title: TAGMENTATION USING IMMOBILIZED TRANSPOSOMES WITH LINKERS 50 1 503 3' 5' 3' biotin B15 502 3' biotin PCR '.17?;5! i5' A14' P i7' B15' 5' 3' P5 i5 Al 4 B15' i7' P 3' • P i5' A14' B15 i7 5' 0 z 3' biotin yj 5' FIG. 5B (57) : The present disclosure relates to methods, compositions, and kits for treating target nucleic acids, including methods 1-1 © and compositions for fragmenting and tagging nucleic acid (e.g., DNA) using transposome complexes bound to a solid support. C [Continued on next page] WO 2018/156519 Al MIDEDIMOMOIDEIREEMOMOMIERHIMEIS (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a))