Patent WO-2019069969-A1 (WO)
Title: Lipid manufacturing method
Inventor: KAWAHARA AKIHITO
IPC: C12P7/64
Filed: 2018-10-03
Abstract: A lipid manufacturing method wherein a transformant into which a gene coding for at least one protein selected from the group consisting of the following proteins (A)-(C) has been introduced is cultured and made to produce a fatty acid or a lipid that has said fatty acid as a constituent component. (A) A protein having acyl-ACP thioesterase activity and comprising an amino acid sequence represented by SEQ ID NO: 1 that has at least one amino acid substitution selected from the group consisting of the amino acid substitutions (A-1) to (A-11) below. (B) A protein having acyl-ACP thioesterase activity and comprising an amino acid sequence that has 85% or more identity with the amino acid sequence represented by SEQ ID NO: 1 and has at least one amino acid substitution selected from the group consisting of the amino acid substitutions (B-1) to (B-11) below. (C) A protein having acyl-ACP thioesterase activity and having the amino acid sequence of protein (A) or (B). (A-1) Isoleucine is substituted for leucine in the 257th place of SEQ ID NO: 1. (A-2) Arginine is substituted for threonine in the 251st place of SEQ ID NO: 1. (A-3) Lysine is substituted for threonine in the 251st place of SEQ ID NO: 1. (A-4) Histidine is substituted for threonine in the 251st place of SEQ ID NO: 1. (A-5) Isoleucine is substituted for tryptophan in the 254th place of SEQ ID NO: 1. (A-6) Tyrosine is substituted for tryptophan in the 254th place of SEQ ID NO: 1. (A-7) Methionine is substituted for leucine in the 257th place of SEQ ID NO: 1. (A-8) Valine is substituted for leucine in the 257th place of SEQ ID NO: 1. (A-9) Phenylalanine is substituted for leucine in the 257th place of SEQ ID NO: 1. (A-10) Cysteine is substituted for valine in the 266th place of SEQ ID NO: 1. (A-11) Tyrosine is substituted for tryptophan in the 271st place of SEQ ID NO: 1. (B-1) Isoleucine is substituted for the amino acid in the location corresponding to the 257th place of SEQ ID NO: 1. (B-2) Arginine is substituted for the amino acid in the location corresponding to the 251st place of SEQ ID NO: 1. (B-3) Lysine is substituted for the amino acid in the location corresponding to the 251st place of SEQ ID NO: 1. (B-4) Histidine is substituted for the amino acid in the location corresponding to the 251st place of SEQ ID NO: 1. (B-5) Isoleucine is substituted for the amino acid in the location corresponding to the 254th place of SEQ ID NO: 1. (B-6) Tyrosine is substituted for the amino acid in the location corresponding to the 254th place of SEQ ID NO: 1. (B-7) Methionine is substituted for the amino acid in the location corresponding to the 257th place of SEQ ID NO: 1. (B-8) Valine is substituted for the amino acid in the location corresponding to the 257th place of SEQ ID NO: 1. (B-9) Phenylalanine is substituted for the amino acid in the location corresponding to the 257th place of SEQ ID NO: 1. (B-10) Cysteine is substituted for the amino acid in the location corresponding to the 266th place of SEQ ID NO: 1. (B-11) Tyrosine is substituted for the amino acid in the location corresponding to the 271st place of SEQ ID NO: 1.