Patent SG-11201811310S-A (SG)
Title: Reagent mixing system and methods
Inventor: WHITACRE JOHANNA L
IPC: G01N24/08
Filed: 2017-12-19
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 11111111111111111111111111 11111111111111111111111111111111111111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\"\" WO 2018/128801 Al 12 July 2018 (12.07.2018) WIP0 1 PCT (51) International Patent Classification: Michael A.; 5200 Illumina Way, San Diego, California G01N 24/08 (2006.01) BOIL 3/00 (2006.01) 92122 (US). DARLAND, Joshua A.; 4881 Willow Road, G01N 35/10 (2006.01) G01N 35/00 (2006.01) #306, Pleasanton, California 94588 (US). ULMANEL- GO1N 35/02 (2006.01) GO1N 35/04 (2006.01) LA, Umberto; 5200 Illumina Way, San Diego, California G01N 35/08 (2006.01) 92122 (US). WANG, Michael Dai; 5200 Illumina Way, (21) International Application Number: San Diego, California 92122 (US). ALVAREZ, Michelle PCT/US2017/067298 L.; 5200 Illumina Way, San Diego, California 92122 (US). CLARK, Stephen Wayne; 5200 Illumina Way, San Diego, (22) International Filing Date: California 92122 (US). 19 December 2017 (19.12.2017) (74) Agent: DIERKER, Julia Church et al.; Dierker & Ka- (25) Filing Language: English vanaugh, P.C., 3331 W. Big Beaver Road, Suite 109, Troy, Michigan 48084 (US). (26) Publication Language: English (81) Designated States (unless otherwise indicated, for every (30) Priority Data: kind of national protection available): AE, AG, AL, AM, 62/442,647 05 January 2017 (05.01.2017) US AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, 1704747.3 24 March 2017 (24.03.2017) GB CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (71) Applicant: ILLUMINA, INC. [US/US]; 5200 Illumina DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, Way, San Diego, California 92122 (US). HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (72) Inventors: WHITACRE, Johanna L.; 5200 Illumina MG, MK, MN, WV, MX, MY, MZ, NA, NG, NI, NO, NZ, Way, San Diego, California 92122 (US). PHELPS, Steven — OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, = S.; 5200 Illumina Way, San Diego, California 92122 (US). SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, DREWS, Bradley Kent; 5200 Illumina Way, San Diego, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. California 92122 (US). BRYAN II, Debra Sue; 5200 Illu- _ mina Way, San Diego, California 92122 (US). NIZIOLEK, = = (54) Title: REAGENT MIXING SYSTEM AND METHODS (57) : A method includes, under control of control circuitry implementing a ( 14 = = - SAMPLE/ LIBRARY PREP S SYTEM i 16 SAMPLE SOURCE i -- M mixing protocol, aspirating reagents from multiple different reagent reservoirs into a 10 cache channel. Designated amounts of the reagents are automatically aspirated from ) the corresponding reagent reservoirs by corresponding sippers based on the mixing = = protocol implemented by the control circuitry. The method also includes discharging i the reagents from the cache channel into a mixing reservoir, and mixing the reagents L OPTICAL DETECTION SYSTEM = Li L26 within the mixing reservoir to form a reagent mixture. 28 FLOW CELL /ARRAY 12 34 36 = L. = 2 38 = VALVING PUMP = 90 VALVING = REAGENTS I DISPOSAL 44.D. ---- - -- - - - - (48 ( 45 #, 11 CONTROL CIRCUITRY -FLUIDICS -I ... -I DATA ACQUISMON ANALYS IS SYSTEM -OPTICS L -50 -STAGE MEMORY CIRCUITRY 11 C.) -OTHER (52I INTERFACE j GC GC I r'l 11 EXTERNAL NETWORKS /SYSTEMS FIG. 1 11 © r'l C [Continued on next page] WO 2018/128801 Al MIDEDIMOMOIDEIREEMOMOHOHHEIROIREM (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — as to the identity of the inventor (Rule 4.17(1)) — as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(11)) Published: — with international search report (Art. 21(3))