Patent SG-11201909017X-A (SG)
Title: Targeted mosquitocidal toxins
Inventor: KEARNEY CHRISTOPHER
IPC: C12N15/82
Filed: 2018-04-03
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 11 October 2018 (11.10.2018) WIPO I PCT o 1E1 oio 0111OH oI0IIIOH01Omuumuu° o (10) International Publication Number WO 2018/187342 Al (51) International Patent Classification: C12N 15/82 (2006.01) C07K 14/18 (2006.01) A01N 63/00 (2006.01) CO7K 14/435 (2006.01) (21) International Application Number: PCT/US2018/025907 (22) International Filing Date: 03 April 2018 (03.04.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/481,199 04 April 2017 (04.04.2017) US (71) Applicant: BAYLOR UNIVERSITY [US/US]; 213 Pat Neff Hall, One Bear Place, Box 97034, Waco, TX 76798-7034 (US). (72) Inventors: KEARNEY, Christopher, Michel; c/o Baylor University, 213 Pat Neff Hall, One Bear Place, Box 97034, Waco, TX 76798-7034 (US). PRUETT, Grace; c/o Baylor University, 213 Pat Neff Hall, One Bear Place, Box 97034, Waco, TX 76798-7034 (US). (74) Agent: BORRELLI, Sara, K. et al.; Jackson Walker LLP, 2323 Ross Avenue, Suite 600, Dallas, TX 75201 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with amended claims (Art. 19(1)) with sequence listing part of description (Rule 5.2(a)) (54) Title: TARGETED MOSQUITOCIDAL TOXINS 100 -0 Toxin = To z -13- Deng ue/Toxin 4- Buffer 50 a) a. ‘ cr 0 0 1 2 3 4 Time (Days) FIG. 6 (57) : Insecticidal toxins described herein are fused toxin peptides made up of a targeting domain fused to a toxin domain. The targeting peptide generates a specific association with mosquitoes by causing the fused toxin peptide to bind mosquitoes in a way that leads to the insecticidal activity. Transgenic plants described herein are mosquitocidal by expressing an insecticidal toxin protein in nectar that includes a targeting peptide to ensure specificity against mosquitoes. These transgenic plants serve as role models for safety, since they are non-crop plants and specific to one mosquito species.