Patent EA-005168-B1 (EA)
Title: Method for inducing resistance to a virus into a cell plant or a plant, cell plant and plant having such resistance
Inventor: JONARD GERARD
IPC: C07K14/08
Filed: 2000-03-07
Abstract: 1. Method for inducing resistance to a group I virus comprising a TGB2 sequence into a plant cell or a plant, comprising the following steps: -preparing a nucleotide construct comprising a nucleotide sequence of said virus or its complementary cDNA, being operably linked to one or more regulatory sequence (s) active in a plant, -transforming a plant cell with the nucleotide construct, and possibly -regenerating a transgenic plant from the transformed plant cell. 2. Method according to the claim 1, characterized in that the group I virus is selected from the group consisting of hordeiviruses, benyviruses, pecluviruses and pomoviruses, preferably selected from the group consisting of the beet necrotic yellow vein virus, the barley stripe mosaic virus, the potato mop top virus, the peanut clump virus and the beet soil-borne virus. 3. Method according to the claim 2, characterized in that nucleotide construct sequence is SEQ ID NO: 3 of BHYVV virus. 4. Method according to any of the preceding claims, characterized in that the plant cell is a stomatal cell. 5. Method according to any of the preceding claims, characterized in that the plant is selected from the group consisting of sugar beet, potato, barley or peanut. 6. Method according to claim 1 or 2, characterized in that the beet necrotic yellow vein virus is BNYVV the nucleotide sequence of TGB2 SEQ ID NO: 1 of said virus is comprised between the nucleotide 3287 and 3643 of the 5'strand of genomic or subgenomic RNA 2 of the BNYVV and the plant is a beet, preferably a sugar beet (Beta vulgaris). 7. Method according to any of the preceding claims, characterized in that the regulatory sequence comprises a promoter sequence or a terminator sequence active in a plant. 8. Method according to claim 7 characterized in that the promoter sequence is a constitutive or a foreigner promoter sequence. 9. Method according to the preceding claim 7, characterized in that the promoter sequence is selected from the group consisting of 35S Cauliflower Mosaic Virus promoter, and/or the polyubiquitin Arabidopsis thaliana promoter. 10. Method according to any of the claim 7 to 9, characterized in that the promoter sequence is a promoter which is capable of being active mainly into the root tissues of plants, such as the par promoter of the haemoglobin gene from Perosponia andersonii. 11. Transgenic plant resistant to a virus obtained by the method characterized in claim 1. 12. Transgenic plant according to the claim 11, characterized in that produced using the nucleotide construct having SEQ ID NO 3 of BNYVV. 13. Transgenic plant according to the claim 11 or 12, characterized in that the virus is selected from the group consisting of hordeiviruses, benyviruses, pecluviruses and pomoviruses, preferably selected from the group consisting of the beet necrotic yellow vein virus, the barley stripe mosaic virus, the potato mop top virus, the peanut clump virus and the beet soil-borne virus. 14. Transgenic plant according to the claims 11 to 13 being a plant selected from the group consisting of sugar beet, potato, barley or peanut. 15. Transgenic plant according to the claims 11 or 12, characterized in that the transgenic plant being a beet, preferably a sugar beet (Beta vulgaris) the virus is BNYVV and the nucleotide sequence of TGB2 SEQ ID NO:1 of said virus is comprised between the nucleotides 3287 and 3643 of the 5'strand of genomic or subgenomic RNA 2 of BNYVV. 16. Transgenic plant according to any of the preceding claims 11 to 15, characterized in that the regulatory sequence comprises a promoter sequence and a terminator sequence active in a plant. 17. Transgenic plant according to any of the preceding claims 11 to 16, characterized in that the regulatory sequence (s) comprise a promoter sequence which is a constitutive or a foreigner promoter sequence. 18. Transgenic plant according to the claim 17, characterized in that promoter sequence is selected from the group consisting of 35S Cauliflower Mosaic Virus promoter, and/or the polyubiquitin Arabidopsis thaliana promoter. 19. Transgenic plant according to claim 17 or 18 characterized in that the promoter sequence is a promoter which is capable of being active mainly into root tissues, such as the par promoter of the haemoglobin gene from Perosponia andersonii. 20. Transgenic plant according to any one of the claims 11 to 19, characterised in that it further carries natural tolerance to viruses characterized in claim 2. 21. Transgenic plant according to any one of the claims 11 to 20, characterised in that it further comprises a pesticide, herbicide or fungicide resistance, preferably a resistance selected from the group consisting of nematode resistance, glyphosate resistance, glufosomate resistance and/or acetochloride resistance. 22. Transformed plant cell obtained by the method characterized in claim 1. 23. Transformed plant cell according to claim 22 from which a transgenic plant can be regenerated characterized in claims 11 to 21.