Patent SG-11201805912X-A (SG)
Title: Method for assessing a state of a living cell
Inventor: GONG TIANXUN
IPC: G01N21/65
Filed: 2017-01-12
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 111111110111101110101011111010111110111011110110111101111111001111101111011111 International Bureau 0.. .... .. ..... ..or::,„, (10) International Publication Number (43) International Publication Date WO 2017/131583 Al 3 August 2017 (03.08.2017) WIP0 I PCT (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every GO1N 21/65 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (21) International Application Number: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, PCT/SG2017/050014 DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (22) International Filing Date: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, 12 January 2017 (12.01.2017) KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, (25) Filing Language: English NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (26) Publication Language: English RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, (30) Priority Data: ZA, ZM, ZW. 10201600721R 29 January 2016 (29.01.2016) SG (84) Designated States (unless otherwise indicated, for every (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY kind of regional protection available): ARIPO (BW, GH, AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, Connexis, Singapore 138632 (SG). TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (72) Inventors: GONG, Tianxun; c/o Singapore Bioimaging TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FL FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, Consortium, 11 Biopolis Way, Helios #01-02, Singapore 138667 (SG). OL1VO, Malini; c/o Singapore Bioimaging LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CL CM, GA, GN, GQ, Consortium, 11 Biopolis Way, Helios #01-02, Singapore _ 138667 (SG). GW, KM, ML, MR, NE, SN, TD, TG). Published: (74) Agent: VIERING, JENTSCHURA & PARTNER LLP; = P.O. Box 1088, Rochor Post Office, Rochor Road, Singa- — with international search report (Art. 21(3)) = pore 911833 (SG). = = = (54) Title: METHOD FOR ASSESSING A STATE OF A LIVING CELL = FIG. 1C = = . .,\ , ,. \,,:v., ,•, . = \ \ . , ,-1 0 old na aparticle .4 en pc lepG2 cell ir) Il M Il ---- (57) : A method for assessing a state of a living cell using surface enhanced Raman spectroscopy (SERS) is provided. The IN m ethod m ay include modifying one or more living eel Is with an alkyne -containing com pound to form one or more modified liv - ing cells, mixing the one or more modified living cells with a SERS-active material to form a m ixture, injecting the m ixture into a N conduitdefmed by an innerwall of a hollow core photonic crystal fiber, and detecting a surface enhanced Raman signal from the mix - O ture in the conduit. In preferred embodiments, the alkyne containing compound is linoleamide alkyne (LLA) for the detection of lip - P. id peroxidation or 4-(dihydroxyborophenyl) acetylene (DBA) for the detection of sialic acid expression in cells, both using gold nan - oparticles as the SERS-active material.