Patent SG-11201907685T-A (SG)
Title: Compositions and methods for robust dynamic metabolic control
Inventor: LYNCH MICHAEL DAVID
IPC: C12N15/09
Filed: 2018-02-21
Abstract: AEAStrain Variants /.. ..tabolic Control ..\\ e Process Robustness f Scalabilily Ornaba. Nutrient n Product • Micro-liter /6 Standardized 2-Stage Process .ana Strain Variants Environmental Variable E vironmental Variable 2 f p • Mi N nit w i:a rkion t i ' Dynamic - N ..., (ii) ,c(7.11)N .(1;i1) . '\ (ii) 8 E vironmenta Environmental Varlode 2 variabie (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 30 August 2018 (30.08.2018) WIP0 I PCT oimiolo VIII °nolo OH VIII VIII 01° milimio oimIE (10) International Publication Number WO 2018/156646 Al (51) International Patent Classification: C12N 1/00 (2006.01) C12N 15/09 (2006.01) C12N 1/21 (2006.01) (21) International Application Number: PCT/US2018/019040 (22) International Filing Date: 21 February 2018 (21.02.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/461,436 21 February 2017 (21.02.2017) US (71) Applicant: DUKE UNIVERSITY [US/US]; 2812 Erwin Rd., Ste 306, Durham, North Carolina 27705 (US). (72) Inventors: LYNCH, Michael David; 2117 Sunset Avenue, Durham, North Carolina 27705 (US). YE, Zhixia; 3515 Ivy Commons Dr., Apt. 301, Raleigh, North Carolina 27606 (US). (74) Agent: ZHANG, MENGMENG; WILSON SONSINI GOODRICH & ROSATI, 650 Page Mill Road, Palo Alto, California 94304 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (54) Title: COMPOSITIONS AND METHODS FOR ROBUST DYNAMIC METABOLIC CONTROL FIGURE 1B (57) : The present disclosure provides compositions and methods for rapid production of chemicals in genetically engineered microorganisms in a large scale. Also provided herein is a high-throughput metabolic engineering platform enabling the rapid optimiza- tion of microbial production strains. The platform, which bridges a gap between current in vivo and in vitro bio-production approaches, © relies on dynamic minimization of the active metabolic network. O [Continued on next page] WO 2018/156646 Al O II TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(11)) as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a))