Patent SG-11201407505X-A (SG)
Title: Aptamer-based multiplexed assays
Inventor: SANDERS GLENN
IPC: C12Q1/68
Filed: 2013-06-07
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau „ (10) International Publication Number (43) International Publication Date ^ ^ WO 2013/185078 A1 12 December 2013 (12.12.2013) WIPO I PCT (51) International Patent Classification: C12Q 1/68 (2006.01) G01N33/543 (2006.01) C07H21/00 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/US2013/044792 7 June 2013 (07.06.2013) English (30) Priority Data: 61/656,956 7 June 2012 (07.06.2012) English US (71) Applicant: SOMALOGIC, INC. [US/US]; 2945 Wilder­ ness Place, Boulder, CO 80301 (US). (72) Inventors: SANDERS, Glenn; 3170 Cripple Creek Trail, Boulder, CO 80305 (US). KRAEMER, Stephan; 4780 West Moorhead Circle, Boulder, CO 80305 (US). KAT- ILIUS, Evaldas; 2184 Jarosa Lane, Superior, CO 80027 (US). (74) Agents: KELLOGG, Rosemary et al.; Swanson & Bratschun, L.L.C., 8210 SouthPark Terrace, Littleton, CO 80120 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) (54) Title: APTAMER-BASED MULTIPLEXED ASSAYS •o © ® n <s 0 5 ra — •- o S | 1 i % E o $ o £• •C <0 Q. +•* c 3 O E < 140 120 j-. 100 o S fc 80 60 40 20 / / / / ^^CAPS eluate Beads y ^^CAPS eluate Beads 1000 2000 3000 4000 00 l> o 00 Amount of biotinylated cold aptamer (fMol) FIG. 1 T-H o CJ o (57) Abstract: The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target mo - lecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantific - ation of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying nucleic a acid (i.e., an aptamer) where the aptamer-aptamer interactions are significantly reduced eliminated while or maintaining the aptamer-target interaction.