Patent SG-11201907936S-A (SG)
Title: Fluidic test cassette
Inventor: THOMAS DONALD
IPC: B01L3/00
Filed: 2018-04-20
Abstract: WO 18/ 195 493 Al FIG. 46A FIG. 468 (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 25 October 2018 (25.10.2018) WIP0 I PCT omit VIII °nolo mu 100 Ill mil ono oimIE (10) International Publication Number WO 2018/195493 Al (51) International Patent Classification: BOIL 3/00 (2006.01) C12Q 1/68 (2018.01) BOIL 7/00 (2006.01) C12Q 1/6804 (2018.01) (21) International Application Number: PCT/US2018/028668 (22) International Filing Date: 20 April 2018 (20.04.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/488,453 21 April 2017 (21.04.2017) US (71) Applicant: MESA BIOTECH, INC. [US/US]; 6181 Cor- nerstone Court, Suite 101, San Diego, CA 92121 (US). (72) Inventors: THOMAS, Donald, J.; 2022 Jewell Ridge, Vista, CA 92081 (US). CAI, Hong; 2306 Canyon Glen Road, Los Alamos, NM 87544 (US). CARY, Robert, B.; 1070 Encantado Drive, Santa Fe, NM 87507 (US). (74) Agent: ASKENAZY, Philip, D.; Peacock Law P.C., P.O. Box 26927, Albuquerque, NM 87125-6927 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3)) (54) Title: FLUIDIC TEST CASSETTE (57) : A disposable cassette for detecting nucleic acids or per- forming other assays. The cassette can be inserted into a base station dur- ing use. The cassette has numerous features to ensure correct operation of the device under gravity, such as vent pockets for enabling the flow of sample fluid from one chamber to the next when the vent pocket is unsealed. The vent pockets have protrusions to help prevent accidental resealing. The cassette also can have a gasket to ensure free air move- ment between open vent pockets. A flexible circuit with patterned metal- lic electrical components disposed on a heat stable material can be in di- rect contact with fluid in the chambers and has resistive heating elements aligned with the vent pockets and the chambers. Recesses in the cassette channels or chambers can have structures such as ridges or grooves to direct fluid flow to enhance rehydration of lyophilized reagents disposed in the recess. Flow diverters in the chambers can reduce the flow veloc- ity of the sample fluid and increase the effective fluid flow path length, enabling more accurate control of fluid flow in the cassette. The roof of each chamber can have a projection that prevents capillary fluid flow across the top of the chamber, thus reducing or preventing sequestratio n of newly resuspended reagent from the bulk of the reaction solution volume.