Patent SG-11201909957T-A (SG)
Title: Engineered ligase variants
Inventor: MILLER MATHEW
IPC: C12N15/31
Filed: 2018-05-07
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 15 November 2018 (15.11.2018) WIPO I PCT ~ill IIIIIIIIII1101DIVIIIIIIIHo o Holommiliu imio oimIE (10) International Publication Number WO 2018/208665 Al (51) International Patent Classification: C12N 9/00 (2006.01) C12N 15/31 (2006.01) C12N 15/09 (2006.01) C12N 15/52 (2006.01) (21) International Application Number: PCT/US2018/031376 (22) International Filing Date: 07 May 2018 (07.05.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/503,075 08 May 2017 (08.05.2017) US 62/540,734 03 August 2017 (03.08.2017) US 62/587,030 16 November 2017 (16.11.2017) US (71) Applicant: CODEXIS, INC. [US/US]; 200 Penobscot Dri- ve, Redwood City, California 94063 (US). (72) Inventors: MILLER, Mathew„ G.; 200 Penobscot Dri- ve, Redwood City, California 94063 (US). VROOM, Jonathan; 200 Penobscot Drive, Redwood City, California 94063 (US). DELLAS, Nikki; 200 Penobscot Drive, Red- wood City, California 94063 (US). BASKERVILLE, Don- ald„ S.; 200 Penobscot Drive, Redwood City, California 94063 (US). GOMES, Sandy„ M.; 200 Penobscot Drive, Redwood City, California 94063 (US). ELGART, David; 200 Penobscot Drive, Redwood City, California 94063 (US). VIDUYA, Judy, Victoria Antonio; 200 Penobscot Drive, Redwood City, California94063 (US). (74) Agent: MACKNIGHT, Kamrin, T.; Codexis, Inc., 200 Penobscot Drive, Redwood City, California 94063 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (54) Title: ENGINEERED LIGASE VARIANTS FIG. 1 Temperature / Activity Profile 90 80 0 70 co 60 -A-SEQ ID NO : 38 0 50 11-WT T4 DNA ligase CU 40 4 , .04 30 7 2 20 15 20 25 30 35 40 Temperature (°C) (57) : The present invention provides engineered ligase polypeptides and compositions thereof, as well as polynucleotides C © encoding the engineered ligase polypeptides. The invention also provides methods for use of the compositions comprising the engineered N • ligase polypeptides for diagnostic and other purposes. [Continued on next page] 0 10 0 00 O 00 WO 2018/208665 Al MIDEDIMOMMIDEFIE1111111111111111111111111101111111111111111111111111 Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a))