Patent SG-11201806335R-A (SG)
Title: Cell culture medium
Inventor: NG SAY KONG
IPC: C12N5/07
Filed: 2017-02-22
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 31 August 2017 (31.08.2017) WIPO I PCT (10) International Publication Number WO 2017/146646 Al 111111111111110111011111111111010111110111010111111111111111111111111111111111110111111 (51) International Patent Classification: C12N 5/07 (2010.01) (21) International Application Number: PCT/SG2017/050079 (22) International Filing Date: 22 February 2017 (22.02.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 10201601284Y 22 February 2016 (22.02.2016) SG (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 Connexis North Tower, Singapore 138632 (SG). (72) Inventors: NG, Say Kong; c/o Bioprocessing Technology Institute, 20 Biopolis Way, #06-01 Centros, Singapore 138668 (SG). LEONG, Sow Zong Dawn; c/o Biopro- cessing Technology Institute, 20 Biopolis Way, #06-01 Centros, Singapore 138668 (SG). TAN, Gek Ling Janice; c/o Bioprocessing Technology Institute, 20 Biopolis Way, #06-01 Centros, Singapore 138668 (SG). TEO, Kah Hui Brian; c/o Bioprocessing Technology Institute, 20 Biopol- is Way, #06-01 Centros, Singapore 138668 (SG). = (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531, Robinson Road Post Office, Singapore 903031 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: with international search report (Art. 21(3)) W O 20 17 / 1466 46 Al (54) Title: CELL CULTURE MEDIUM (57) : The invention relates to serum-free cell culture medium, wherein the medium either contains maltose as the sole car- bohydrate source, or contains maltose and at least one other saccharide as carbohydrate sources. In a preferred embodiment, the ad- ditional saccharide is a monosaccharide, preferably glucose, and the medium comprises DMEM-F12. In a preferred embodiment the cells to be cultured may be CHO or HEK293 cells. Also disclosed are methods of growing and/or culturing a cell using the cell cul- ture medium as described herein, methods of increasing protein yield using the cell culture medium as described herein, and kits thereof.