Patent SG-11201908999Q-A (SG)
Title: Self-assembling protein nanostructures displaying paramyxovirus and/or pneumovirus f proteins and their use
Inventor: KING NEIL
IPC: C12N15/63
Filed: 2018-04-03
Abstract: Antigennann._ *c e timer fusion 3F In vitro assembly Nanoi..4rticie p etamer (E coil) Desigret, w loparticie immunogen (20 tamers 17.C.1 (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 11 October 2018 (11.10.2018) WIPO I PCT oimiolollm OH oI0IIIOHmom!!D IOHIIII111110 OIIIIII (10) International Publication Number WO 2018/187325 Al (51) International Patent Classification: C07K 14/195 (2006.01) Cl 2N 15/63 (2006.01) C07K 14/135 (2006.01) Cl 2N 5/10 (2006.01) C07K 14/115 (2006.01) A61K 39/155 (2006.01) C12N 15/62 (2006.01) B82Y 5/00 (2011.01) (21) International Application Number: PCT/US2018/025880 (22) International Filing Date: 03 April 2018 (03.04.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/481,331 04 April 2017 (04.04.2017) US (71) Applicants: UNIVERSITY OF WASHINGTON [US/US]; 4545 Roosevelt Way NE, Suite 400, Seattle, WA 98105-4721 (US). INSTITUTE FOR RESEARCH IN BIOMEDICINE [CH/CH]; Via Vincenzo Vela 6, 6500 Bellinzona (CH). (72) Inventors: KING, Neil, P.; c/o University of Washing- ton, 4545 Roosevelt Way NE, Suite 400, Seattle, WA 98105-4721 (US). BAKER, David; c/o University of Washington, 4545 Roosevelt Way NE, Suite 400, Seat- tle, WA 98105-4721 (US). NICKERSON, Brooke; c/o University of Washington, 4545 Roosevelt Way NE, Suite 400, Seattle, WA 98105-4721 (US). STEWART, Lance, Joseph; c/o University of Washington, 4545 Roosevelt Way NE, Suite 400, Seattle, WA 98105-4721 (US). PEREZ, Laurent; c/o Institute for Research in Biomedicine, Via Vincenzo Vela 6, CH-6500 Bellinzona (CH). LANZA- VECCHIA, Antonio; c/o Institute for Research in Bio- medicine, Via Vincenzo Vela 6, CH-6500 Bellinzona (CH). MARCANDALLI, Jessica; c/o Institute for Research in Biomedicine, Via Vincenzo Vela 6, CH-6500 Bellinzona (CH). = (54) Title: SELF-ASSEMBLING PROTEIN NANOSTRUCTURES DISPLAYING PARAMYXOVIRUS AND/OR PNEU- MOVIRUS F PROTEINS AND THEIR USE cc 00 O C (57) : Disclosed herein are nanostructures and their use, where the nanostructures include (a) a plurality of first assemblies, each first assembly comprising a plurality of identical first polypeptides; (b) a plurality of second assemblies, each second assembly comprising a plurality of identical second polypeptides, wherein the second polypeptide differs from the first polypeptide; wherein the plurality of first assemblies non-covalently interact with the plurality of second assemblies to form a nanostructures; and wherein the nanostructure displays multiple copies of one or more paramyxovirus and/or pneumovirus F proteins or antigenic fragments thereof, on an exterior of the nanostructure. [Continued on next page] WO 2018/187325 Al MIDEDIMOMOIDEIREEMOMOHOHIIMMOMEHIE (74) Agent: HARPER, David, S.; McDonnell Boehnen Hulbert & Berghoff LLP, 300 South Wacker Drive, Chicago, IL 60606 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a))