Patent EA-002180-B1 (EA)
Title: Dna molecules of intergenic regions of banana bunchy top virus, method of expressing a non-bbtv gene in a plant cell using said dna molecules and transformed vegetal cell line, comprising dna molecules
Inventor: DALE JAMES LANGHAM
IPC: C12N15/82
Filed: 1996-05-31
Abstract: 1. A DNA molecule which comprises the nucleotide sequence which is complementary to the sequence of an intergenic region of a 1 BBTV component shown at Fig.11 as bbtvpro 1 (pBT 1.1) sequence at least for 80%, and coding the promoter capable of transcription of a non-BBTV gene. 2. A DNA molecule which comprises the nucleotide sequence which is complementary to the sequence of an intergenic region of a 2 BBTV component shown at Fig.11 as bbtvpro 2 (pBT 2.1) sequence at least for 80%, and coding the promoter capable of transcription of a non-BBTV gene. 3. A DNA molecule which comprises the nucleotide sequence which is complementary to the sequence of an intergenic region of a 3 BBTV component shown at Fig.11 as bbtvpro 3 (pBT 3.1) sequence at least for 80%, and coding the promoter capable of transcription of a non-BBTV gene. 4. A DNA molecule which comprises the nucleotide sequence which is complementary to the sequence of an intergenic region of a 4 BBTV component shown at Fig.11 as bbtvpro 4 (pBT 4.1) sequence at least for 80%, and coding the promoter capable of transcription of a non-BBTV gene. 5. A DNA molecule which comprises the nucleotide sequence which is complementary to the sequence of an intergenic region of a 5 BBTV component shown at Fig.11 as bbtvpro 5 (pBT 5.1) sequence at least for 80%, and coding the promoter capable of transcription of a non-BBTV gene. 6. A DNA molecule which comprises the nucleotide sequence which is complementary to the sequence of an intergenic region of a 6 BBTV component shown at Fig.11 or 13 (a) as bbtvpro 6 (pBT 6.1) sequence at least for 80%, and coding the promoter capable of transcription of a non-BBTV gene. 7. A DNA molecule which comprises the nucleotide sequence which is complementary to the sequence pBT 6.2 shown at Fig.13 (t) at least for 80%, coding the promoter capable of transcription of a non-BBTV gene. 8. A DNA molecule which comprises the nucleotide sequence which is complementary to the sequence pBT 6.3 shown at Fig.13 (c) at least for 80%, coding the promoter capable of transcription of a non-BBTV gene. 9. A DNA molecule which comprises the nucleotide sequence which is complementary to the sequence pBT 6.4 shown at Fig.13 (d) at least for 80%, coding the promoter capable of transcription of a non-BBTV gene. 10. A DNA molecule according to any of Claims 1-9, characterised in that it is capable of transcription of a non-BBTV gene in monocotyledonous plant cells or in such plant. 11. A DNA molecule according to any of Claims 1-9, characterised in that it is capable of transcription of a non-BBTV gene in dicotyledonous plant cells or in such plant. 12. A DNA molecule according to any of Claims 1-9, which is capable of transcription of a non-BBTV gene in undifferentiated tissue of a dicotyledon plant. 13. A method of expressing a non-BBTV gene in a plant cell comprising obtaining a sample comprising fixed DNA molecule according to any of Claims 1-9; obtaining a vegetal cell preparation in such conditions, which make possible an introduction of DNA molecule into vegetal cell and  induction of a non-BBTV gene expression in vegetal cell, comprising said DNA molecule. 14. A transformed vegetal cells line, comprising DNA molecule according to any of Claims 1-9.