Patent SG-11201903024Y-A (SG)
Title: Improved process for the production of fucosylated oligosaccharides
Inventor: JENNEWEIN STEFAN
IPC: C12N9/10
Filed: 2017-10-24
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1 MEM 0 11101 HOE HI 1 0 0111101110 Ell 1101E1 IMO III OEN Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\" WO 2018/077892 Al 03 May 2018 (03.05.2018) WIP0 I PCT (51) International Patent Classification: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, C12P 19/18 (2006.01) Cl 2N 9/10 (2006.01) CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, C12N 1/21 (2006.01) Cl 2N 9/12 (2006.01) DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (21) International Application Number: HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, PCT/EP2017/077192 KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (22) International Filing Date: OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, 24 October 2017 (24.10.2017) SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (25) Filing Language: English (26) Publication Language: English (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (30) Priority Data: GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, 16196486.1 29 October 2016 (29.10.2016) EP UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, (71) Applicant: JENNEWEIN BIOTECHNOLOGIE EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, GMBH [DE/DE]; Maarweg 32, 53619 Rheinbreitbach MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (DE). TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, (72) Inventors: JENNEWEIN, Stefan; Wichfriedweg 14, KM, ML, MR, NE, SN, TD, TG). 53604 Bad Honnef (DE). WARTENBERG, Dirk; Her- — mannstrasse 102, 53225 Bonn (DE). PARSCHAT, Katja; Published: Jahnallee 43, 53173 Bonn (DE). — with international search report (Art. 21(3)) = — with sequence listing part of description (Rule 5.2(a)) (74) Agent: WITTE, WELLER & PARTNERPATENTAN- WALTE = MBB; Postfach 10 54 62, 70047 Stuttgart (DE). (81) Designated States (unless otherwise indicated, for every = kind of national protection available): AE, AG, AL, AM, = = (54) Title: IMPROVED PROCESS FOR THE PRODUCTION OF FUCOSYLATED OLIGOSACCHARIDES = = = = _ — 0 1 0---p —o 1 7N H2OH ManA ManB ManC Gmd WcaG HO LW = pp HaC - 0 , X OH .0 LacY)— OH OH lactose lactose = = = = fructose-6 PfkA -phosphate GDP-L-fucose WbgL St Fbpase H HO H OH OH HO OH = Gl Ho O. 1 -OH TP Yb Ho --•.--i- r pF glycerol glyceraldehyde-3-phosphate Il 4 Glycolysis 6 OH / 2'-fucosyllactose 2'-fucosyllactose ei tT ot IN Fig , 1 IN © 'i t - • • (57) : The present invention relates to a method for producing fucosylated oligosaccharides by using a recombinant prokaryotic Il host cell that is cultivated on a gluconeogenic substrate, as well as to the host cell and its use. The host cell is genetically modified in 0 el that the activity of a fructose-6-phosphate converting enzyme is abolished or lowered, and the transport of the produced fucosylated oligosaccharide through the cell membrane is facilitated by an exogenous transport protein.