Patent SG-11201810915Q-A (SG)
Title: Preparation of factor xa derivatives
Inventor: KARBARZ MARK
IPC: C07K1/22
Filed: 2017-06-19
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 21 December 2017 (21.12.2017) WIP0 I PCT omit VIII two III OH Oil moimommo (10) International Publication Number WO 2017/219034 A2 (51) International Patent Classification: C12N 9/64 (2006.01) CO7K 1/22 (2006.01) (21) International Application Number: PCT/US2017/038169 (22) International Filing Date: 19 June 2017 (19.06.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/351,841 17 June 2016 (17.06.2016) US (71) Applicant: PORTOLA PHARMACEUTICALS, INC. [US/US]; 270 E. Grand Ave., Suite 22, South San Francis- co, California 94080 (US). (72) Inventors: KARBARZ, Mark; c/o Portola Pharmaceuti- cals, Inc., 270 E. Grand Ave., Suite 22, South San Fran- cisco, California 94080 (US). CONLEY, Pamela B.; c/o Portola Pharmaceuticals, Inc., 270 E. Grand Ave., Suite 22, South San Francisco, California 94080 (US). LU, Genmin; c/o Portola Pharmaceuticals, Inc., 270 E. Grand Ave., Suite 22, South San Francisco, California 94080 (US). (74) Agent: NIE, Alex Y. et al.; SHEPPARD MULLIN RICHTER & HAMPTON LLP, 379 Lytton Avenue, Palo Alto, California 94301-1479 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) Published: without international search report and to be republished upon receipt of that report (Rule 48.2(g)) O N O (54) Title: PREPARATION OF FACTOR XA DERIVATIVES (57) : The present disclosure provides methods for manufacturing a fXa derivative protein at large scale leading to high yield of highly pure protein product. The method may include adding a detergent to a sample that contains a polynucleotide construct encoding the protein and purifying the protein through a soybean trypsin inhibitor (STI)-based affinity chromatograph, an ion exchange and mixed mode chromatograph and a hydrophobic interaction.