Patent SG-11201907202W-A (SG)
Title: Integrated multi-electrode array and trans-endothelial electrical resistance in organ-on-a-chip microsystems
Inventor: MAOZ BEN
IPC: G01N27/40
Filed: 2018-02-26
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 30 August 2018 (30.08.2018) WIPO I PCT 0111111010 0111 °nolo OH loomonmll nio oimIE (10) International Publication Number WO 2018/157073 Al (51) International Patent Classification: BOIL 3/00 (2006.01) Cl 2N 5/07 (2010.01) C12M 3/04 (2006.01) G01N 27/40 (2006.01) (21) International Application Number: PCT/US2018/019754 (22) International Filing Date: 26 February 2018 (26.02.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/464,229 27 February 2017 (27.02.2017) US 62/506,420 15 May 2017 (15.05.2017) US (71) Applicant: PRESIDENT AND FELLOWS OF HAR- VARD COLLEGE [US/US]; 17 Quincy Street, Cam- bridge, Massachusetts 02138 (US). (72) Inventors: MAOZ, Ben, M.; 54 Moritz Daniel St., Apt. 1, Tel Aviv (IL). HERLAND, Anna; Hemmansgatan 76, 583 36 Linkoping (SE). HENRY, Olivier, F.; 519 Washing- ton Street, Apt. 15, Brookline, Massachusetts 02446 (US). PARKER, Kevin, K.; 29 Oxford St., Cambridge, Massa- chusetts 02138 (US). INGBER, Donald, E.; 162 W. Can- ton St., Boston, Massachusetts 02118 (US). (74) Agent: RESNICK, David, S. et al.; Nixon Peabody LLP, 70 W. Madison St., Suite 3500, Chicago, Illinois 60602-4224 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. = (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) 1-1 N O N kr) 1-1 00 1-1 O N C (54) Title: INTEGRATED MULTI-ELECTRODE ARRAY AND TRANS-ENDOTHELIAL ELECTRICAL RESISTANCE IN OR- GAN-ON-A-CHIP MICROSYSTEMS (57) : An organ-on-chip device for monitoring a biological function and including a membrane layer located at an interface region between a top microchannel and the a microchannel. The membrane includes a first type of cells forming a barrier between the top microchannel and the bottom microchannel. The device further includes a top layer having a first plurality of transendothelial electrical resistance (TEER) measurement electrodes for enabling direct monitoring of cell function and electrical activity of the first type of cells on the membrane. The device also has a multi-electrode array (MEA) layer with a second plurality of TEER measurement electrodes for enabling direct monitoring of cell function and electrical activity of a second type of cells on the MEA layer.