Patent SG-11201906553T-A (SG)
Title: Methods and reagents for synthesising polynucleotide molecules
Inventor: MILTON JOHN
IPC: C12Q1/6844
Filed: 2018-01-19
Abstract: VoodooI Method . Depcoixetlon (106,110) 1. Provision of scaffold two b Un 2. Incorporation (102, 107) w UnX wow s Support strand bls tossarksior gm* b Synthesis skied (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 26 July 2018 (26.07.2018) WIP0 I PCT omit IIl °nolo Oil IIII 1111101 ois (10) International Publication Number WO 2018/134616 Al (51) International Patent Classification: BO1J 19/00 (2006.01) C12Q 1/6844 (2018.01) (21) International Application Number: PCT/GB2018/050165 (22) International Filing Date: 19 January 2018 (19.01.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 1700937.4 19 January 2017 (19.01.2017) GB (71) Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED [GB/GB]; Suite 14 Science Village, Chester- ford Research Park, Cambridge Cambridgeshire CB10 1XL (GB). (72) Inventors: MILTON, John; Oxford Nanopore Technolo- gies Ltd., Suite 14 Science Village, Chesterford Research Park, Cambridge Cambridgeshire CB10 1XL (GB). NAY- YAR, Sobia; Oxford Nanopore Technologies Ltd., Suite 14 Science Village, Chesterford Research Park, Cambridge Cambridgeshire CB10 1XL (GB). RIEDL, Jan; Oxford Nanopore Technologies Ltd., Suite 14 Science Village, Chesterford Research Park, Cambridge Cambridgeshire CB10 1XL (GB). OGAKI, Ryosuke; Oxford Nanopore Technologies Ltd., Suite 14 Science Village, Chesterford Research Park, Cambridge Cambridgeshire CB10 1XL (GB). (74) Agent: WILKINSON, Marc George; J A Kemp, 14 South Square, Gray's Inn, London Greater London WC1R 5JJ (GB). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (54) Title: METHODS AND REAGENTS FOR SYNTHESISING POLYNUCLEOTIDE MOLECULES (57) : The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide 0 sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems \" and kits for performing the synthesis and/or assembly methods. C [Continued on next page] WO 2018/134616 Al MIDEDIMOMOIDEIREEMOMMIONHOI111111111110111111111111 GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a))