Patent SG-11201900270Y-A (SG)
Title: Method of quantitative measurement of particle content using hydrated state imaging
Inventor: COLOMB-DELSUC MATHIEU
IPC: G01N15/02
Filed: 2018-01-25
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property MD 1101111 01E101011M OH H 1 0 111 vii 1001101 vii Mil EMI lil Oil lil Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\" WO 2018/160298 Al 07 September 2018 (07.09.2018) WI P0 I PCT — (51) (21) (22) (25) Filing Language: (26) (30) (71) (72) International Patent Classification: Banslattsvagen 7A, SE 146 37 Tullinge (SE). RYNER, C07K 14/005 (2006.01) C07K 14/015 (2006.01) Martin; Glasbarsvagen 16, SE 141 68 Huddinge (SE). C07K 14/01 (2006.01) G01N 15/02 (2006.01) (74) Agent: FASTH, Rolf; FASTH LAW OFFICES, 1206 Stan- International Application Number: ridge Drive, Raleigh, North Carolina 27613 (US). PCT/US2018/015263 (81) Designated States (unless otherwise indicated, for every International Filing Date: kind of national protection available): AE, AG, AL, AM, 25 January 2018 (25.01.2018) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, English DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, Publication Language: English HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, Priority Data: KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 62/465,981 02 March 2017 (02.03.2017) US MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, Applicant: INTELLIGENT VIRUS IMAGING INC. SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, [US/US]; 26 Pinecrest Plaza, Suite 2, Southern Pines, North TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Carolina 28387 (US). (84) Designated States (unless otherwise indicated, for every Inventors: COLOMB-DELSUC, Mathieu; Liljestigen kind of regional protection available): ARIPO (BW, GH, 11 a, SE 125 53 Alvsjo (SE). HAAG, Lars; Furuborgsvagen GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, 3A, SE 155 32 Nykvarn (SE). NORDSTROM, Rickard; UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, = = Title: METHOD OF QUANTITATIVE 102 : The method is for quantitative (100) of virus-like particles (VLPs) or virus particles is provided. Preferably, the at a cryogenic temperature. While at the cryogenic temperature, the particle content the CryoTEM. An amount of the particle content of the VLPs is determined to assess (54) (57) MEASUREMENT OF PARTICLE CONTENT USING HYDRATED STATE IMAGING 106 7 -100 104 hydrated state imaging such as CryoTEM. A sample (100) is rapidly frozen into a cryogenic of each VLP in the frozen sample is observed whether the VLPs are empty or not. = = = = 1110 4i!:' 0 o 0 0 't, 0 L—.I = = = = = = = = = _ 22nm 11 GC c:r N © 1-1 ---- sample GC liquid 1-1 © in ei 108 FIG. 1 measurement of particle content using O [Continued on next page] WO 2018/160298 Al MIDEDIMOMMIDIREEMOMOHINIEEMEHMEMOIMIE EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, SK, SM, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with amended claims (Art. 19(1))