Patent SG-11201903034V-A (SG)
Title: Method of cultivation of human salivary gland cells
Inventor: Petrakova Olga Sergeevna
IPC: C12N5/00
Filed: 2017-10-03
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property MD 1101111 0 1110101011111 3E1 0 011101 100 MIMI OHRE IIE OEN Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\" WO 2018/067036 Al 12 April 2018 (12.04.2018) WIP0 1 PCT (51) International Patent Classification: TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, C12N 5/00 (2006.01) EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (21) International Application Number: MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, PCT/RU2017/000736 TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (22) International Filing Date: 03 October 2017 (03.10.2017) Published: with international search report (Art. 21(3)) (25) Filing Language: English 1 before the expiration of the time limit for amending the (26) Publication Language: English claims and to be republished in the event of receipt of (30) Priority Data: amendments (Rule 48.2(h)) 2016139283 06 October 2016 (06.10.2016) RU (71) Applicant: PIROGOV RUSSIAN NATIONAL RESEARCH MEDICAL UNIVERSITY (RNRMU) [RU/RU]; ul. Ostrovityanova, 1, Moscow, 117997 (RU). (72) Inventors: PETRAKOVA, Olga Sergeevna; ul. Rech- naya, 12, kv. 54, Balashikha, 143981 (RU). BORISOV, Mikhail Aleksandrovich; ul. Myachnikovskij bulvar, 27, kv. 194, Moscow, 109469 (RU). VOROTELYAK, Eka- terina Andreevna; ul. Vavilova, 76, kv. 45, Moscow, 119261 (RU). GVAZAVA, Inessa Givievna; Kolomen- skaya naberezhnaya, 10, kv. 224, Moscow, 115142 (RU). ROGOVAYA, Olga Sergeevna; Yubileinyj prospect, 52, kv. 141, Reutov, 143969 (RU). VASILIEV, Andrey ul. Valentinovich; Malaya Ostroumovskaya, 1B, kv. 246, Moscow, 107014 (RU). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind ARIPO GH, of regional protection available): (BW, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, ,_ 1 (54) Title: METHOD OF CULTIVATION OF HUMAN SALIVARY GLAND CELLS (57) : The present invention is intended to increase the number of human salivary gland cell passages, maintain their undif- C> ferential condition and high proliferative potential during cultivation. The culture method of human salivary gland epithelial progenitor C cells comprising: (a) obtaining human salivary gland epithelial progenitor cells from recipient organism; (b) cell transfer into PCT N Epidermal Keratinocyte Medium and cultivation in culture flasks ensuring cell adhesion at 37°C with addition of 5% CO 2 and medium change every 2-4 days until monolayer is reached; (c) cell passage at 1:3-1:5 dilution ratio, including cell removal from the culture flask ---- surface using EDTA trypsin solution and transfer into the new culture flasks; (d) further cell cultivation as defined in claim (b) with in- c)1: 1-1 process medium change every 2-4 days and passaging until monolayer is reached, as defined in claim (c) at a maximum dilution ratio © of 1:2-1:3, where the first medium change after each passage shall be provided within 8-24 hours. ei C