Patent SG-11201901411V-A (SG)
Title: Conditionally active polypeptides and methods of generating them
Inventor: SHORT JAY
IPC: G01N33/574
Filed: 2017-08-22
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1#11101111 0 11101 HOE HI 1 0 011101 11101111011 01111101111011 OEN Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\"\"1 WO 2018/044619 Al 08 March 2018 (08.03.2018) WIP0 I PCT (51) International Patent Classification: (72) Inventors: SHORT, Jay, M.; 12985 Via Esperia, Del Mar, C07K 16/18 (2006.01) G01N 33/574 (2006.01) CA 92014 (US). CHANG, Hwai, Wen; 1318 Shadow Hills C07K 16/28 (2006.01) A61K 39/00 (2006.01) Drive, San Marcos, CA 92069 (US). FREY, Gerhard; (21) International Application Number: 13768 Via Cima Bella, San Diego, CA 92129 (US). PCT/US2017/047950 (74) Agent: DUNLEAVY, Kevin, J.; Mendelsohn Dunleavy, (22) International Filing Date: P.C., 1500 John F. Kennedy Blvd., Suite 312, Philadelphia, PA 19102 (US). 22 August 2017 (22.08.2017) (25) Filing Language: English (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, (26) Publication Language: English AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (30) Priority Data: CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, 62/382,023 31 August 2016 (31.08.2016) US DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, (71) Applicant: BIOATLA, LLC [US/US]; 11011 Torreyana KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, Road, San Diego, CA 92121 (US). MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (54) Title: CONDITIONALLY ACTIVE POLYPEPTIDES AND = = ist ASTR = — % = nd ASTR = = = = = = = = = = - 11 (57) : A method of preparing a conditionally active polypeptide CN DNA encoding the parent polypeptide by increasing a net charge 1-1 increasing a total number of codons of charged amino acid residues il• amino acid residues in the DNA to create mutant DNAs; expressing C conditionally active polypeptide from the mutant polypeptides which exhibits a decrease in activity in a first assay at a first value of a —. condition compared to the same activity in a second assay at a second GC pharmaceutical compositions containing same, nanoparticle and drug 1-1 0 ei FIG. of the parent polypeptide using one or more techniques selected from in the DNA and decreasing a total number of codons of uncharged the METHODS OF GENERATING THEM value conjugates thereof and uses thereof are also provided. D M CSD SD 5 from a parent polypeptide, comprising steps of evolving a mutant DNAs to obtain mutant polypeptides; and selecting the of the same condition. The conditionally active polypeptide, O [Continued on next page] WO 2018/044619 Al IMEDIM011111 11111111EEM0MMOIMI111101111101111111111111111111 SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3))