Patent DE-102010046199-A1 (DE)
Title: Three-piece centrifuge, useful for the local fixation of ribonucleic acid- and deoxyribonucleic acid sequencing, comprises a bottom-mounted overflow dish and a rotational shell with a rotating lid
Inventor: KRAEBER BERNHARD
IPC: C12Q1/68
Filed: 2010-09-21
Abstract: Three-piece centrifuge comprises a bottom-mounted overflow dish and a rotational shell (1) with a rotating lid (2). The overflow dish has its upper extremities above the slit formed of lid and rotational shell. The overflow dish ends in the lower extremities on cylindrical outer wall of the case. The overflow dish is executed in the upper region of the beam passage from optically neutral color material. A groove is milled on the outer extreme of the rotational shell for the temporary closure with the lid. A rough metal surface (7) is deposited or sintered to the left of the groove. Three-piece centrifuge comprises a bottom-mounted overflow dish and a rotational shell (1) with a rotating lid (2). The overflow dish has its upper extremities above the slit formed of lid and rotational shell. The overflow dish ends in the lower extremities on cylindrical outer wall of the case. The overflow dish is executed in the upper region of the beam passage from optically neutral color material. A groove is milled on the outer extreme of the rotational shell for the temporary closure with the lid. A rough metal surface (7) is deposited or sintered to the left of the groove, and polished metal surface (8) is deposited or sintered to the right of the groove. A cone-shaped opening at the top tapering upward towards the axis is present in the lower extreme of the rotational shell. The cone flattened with flanged outer axis for receiving the inner axis is present in the upper extreme. A vertical groove for receiving a vertically installed wedge is inserted in the upper region of the outer axis, which connects the outer axis with the upper inner axis with vertical fixture. The induction zone is received in the lower region of the cone and uniformly arranged rod-shaped permanent magnet is introduced in the upper region of the cone. The lid with inner axis, comprises four segments of the axis extending outwardly from inner segment, and a descending from inside to outside and downwards. Independent claims are also included for: (1) a housing for accommodating the centrifuge, comprising the flanged feed pump, an injection duct, a cylinder (11), an induction coil, latched electric coil, and a ring pump centrally mounted with valve; (2) a laser variant of stimulated anti-Stokes Raman scattering, where three or four Stokes laser time-shifts are formed in the junction of the first pump laser to achieve three or four frequency differences, where one of the frequency differences correspond to the respective molecular species of the natural frequency; (3) separating and fixing the DNA, comprising accelerating and decelerating the rotational speed of rotational shell appropriately, varying the temperature and fixing the analyst (DNA or RNA) after exposure to nitrogen in the gap between the rotational shell and raised lid, generating temperature gradients during moderate rotation of the rotational shell to form a spiral rotation of the DNA and to spatially separate single strands from one another, and using an analog-useable shock wave method, which separates DNA strand into two single strands at moderate rotation of the rotational shell; and (4) generating a radioactively labeled complementary single strands, comprising (a) adding the purified chromosome to a rotating dish, which is inserted at low speed into a medium in a stretched bent state, where the medium behaves laminarly in the rotational shell, and twists and tangles of analyst are excluded, (b) denaturing of the double strand by raising the temperature to 94[deg] C (varies with the cytosine, guanine of the DNA) by the induction coil, (c) spatially separating the single strands by thermal gradients after previous cooling or by shock wave method at low rotation speed and then increasing the speed gradually, using denatured single strands for hybridizing a primer and radioactively labeled nucleotides, which are injected through the injection duct, removing the recognizable radioactively labeled double strands from the rotating dish, removing the medium with the disturbing excess primers and the deoxynucleotide triphosphates from the rotational shell and replacing with a purified medium, feeding back one of the two double strands to the rotational shell, removing remaining radioactively labeled double-strands to be denature and the radioactively labeled single-strand from the rotational shell.