Patent SG-11201909954Y-A (SG)
Title: Methods for culturing human keratinocytes
Inventor: TRYGGVASON KARL
IPC: C12N5/071
Filed: 2018-05-04
Abstract: kkkk r I • n* ri : 4 3T3 Ln-511 Ln-421 O 00 O C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 08 November 2018 (08.11.2018) WIPO I PCT IiiimmomitiaonolooloionommoommovoimIE (10) International Publication Number WO 2018/203834 Al (51) International Patent Classification: C12N 5/071 (2010.01) G01N 33/50 (2006.01) A61K 35/36 (2015.01) (21) International Application Number: PCT/SG2018/050220 (22) International Filing Date: 04 May 2018 (04.05.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/501,992 05 May 2017 (05.05.2017) US (71) Applicants: NATIONAL UNIVERSITY OF SINGA- PORE [SG/SG]; 21 Lower Kent Ridge Road, Singapore 119077 (SG). SINGAPORE HEALTH SERVICES PTE LTD [SG/SG]; 31 Third Hospital Avenue, Bowyer Block C, #03-03, Singapore 168753 (SG). (72) Inventors: TRYGGVASON, Karl; c/o Duke-NUS Med- ical School, 8 College Road, Singapore 169857 (SG). TJIN, Monica Suryana; c/o Duke-NUS Medical School, 8 College Road, Singapore 169857 (SG). CHUA, Alvin Wen Choong; c/o Singapore Health Services Pte Ltd, 31 Third Hospital Avenue, Bowyer Block C,#03-03, Singapore 168753 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531, Robinson Road Post Office, Singapore 903031 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (54) Title: METHODS FOR CULTURING HUMAN KERATINOCYTES 120- 100- Ty 80- O o. 20- 0 60- 40- KRT5 KRT14 KRT15 ITGA6 ITG B1 KRT1 KRT10 I I I Basal markers Differentiation markers FIG. 5 (57) : The present disclosure relates to methods for culturing human epidermal keratinocytes. When keratinocytes are cultured on plates coated with a laminin containing an alpha-4 chain or an alpha-5 chain, in a xeno-free, chemically defined cell culture medium, they expand efficiently in vitro. Useful cell culture kits for culturing keratinocytes are also described herein, as are methods of using such cells for treatment of burns or chronic wounds. [Continued on next page] WO 2018/203834 Al MIDEDIMOMOIDEIRDERI0010101110MMOMMEMOIS UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: of inventorship (Rule 4.17 (iv)) Published: with international search report (Art. 21(3)) in black and white; the international application as filed contained color or greyscale and is available for download from PATENTSCOPE