Patent DE-102006032953-A1 (DE)
Title: Biosensor blank for production of biosensor arrangement for amperometric and/or potentiometric testing of active- site/substances from pharmacological point of view, comprises an electrode and primary carrier for in-vitro-translation
Inventor: KELETY BELA
IPC: C12Q1/02
Filed: 2006-07-17
Abstract: The biosensor blank for the production of biosensor arrangement for amperometric and/or potentiometric testing of active site or substances from pharmacological point of view, comprises an electrical conductive electrode (26), a primary carrier (10) intended in electrically capacitive coupling for in-vitro-translation/transcription, an activable biological unit formed in adjustable type and manner over the process of in-vitro-translation to an electrical action, and ribosomal units intended on or in the primary carrier for practicability or for the execution of the in-vitro-translation. The biosensor blank for the production of biosensor arrangement for amperometric and/or potentiometric testing of active site or substances from pharmacological point of view, comprises an electrical conductive electrode (26), a primary carrier (10) intended in electrically capacitive coupling for in-vitro-translation/transcription, an activable biological unit formed in adjustable- type and manner over the process of in-vitro-translation to an electrical action, and ribosomal units intended on or in the primary carrier for practicability or for the execution of the in-vitro-translation. The ribosomal unit has a ribosome. The primary carrier is a membrane fragment of a visicel or liposome diverted from an endoplasmatic reticulum. The visicel, liposome, micellar structure and/or its component, fragments, membrane fragments or constituents are formed as the primary carrier in native form, in modified form, in cleaned form, in microbiologically changed form or in molecular biologically changed form. The primary carrier has a membrane (11) with inner side (11a) and outer side (11b). The ribosomal unit is formed on and/or in the membrane of the carrier at or with access on the outside. An activable biological, chemical and/or biochemical unit, a transport unit and/or their components, fragments and/or constituents are formable through the in-vitro-translation as a biological unit to partial electrogene load carrier transport or load carrier movement. An artificial polypeptide chain, membrane protein, an ions pump, an ions channel, a transporter or a receptor are formable through the in-vitro-translation as biological unit, which is formable in native form, modified form, cleaned form, microbiologically changed form and/or molecular biologically changed form. The carrier is intended in direct spatial neighborhood of the electrode, which is arranged opposite to the carrier in electrically isolated manner and is formed as solid body support. The solid body like carrier is intended with a surface. The electrode is formed as a related material layer on the surface of the solid body like carrier, which has an electrically isolated and/or chemically inerted material or which is formed out of glass, plastic or polymer. The electrode is formed as vacuum-metallized or sputtered material layer on the carrier surface with a layer strength of 10-200 nm and is electrically isolated or isolatable through multilayered isolation areas (24), which are intended for mechanical contact with the primary carrier, a measuring medium and/or synthesis medium. The isolation area is partially formed as spontaneous self-organizing layer or as self assembling layer. A layer out of octadecanthiol is intended as lower layer or as under layer and a layer out of lipids is intended as upper layer or upper most layers turned away from the electrode or upper surface of the isolation area. The under layer is formed with a layer out of Teflon (RTM: polytetrafluoroethylene), plastic or polymer with a layer thickness of less than 5 mu m. An electrode substrate is formed with a surface for the electrode and from electrical conductive plastic or polymer material. The isolation area is formed with a biomaterial area in or on the surface of the electrode substrate. The electric conductivity of the plastic or the polymer material is formed through providing a first extra charge material, which is formed out of carbon in the form of soot, graphite, nanoparticle, buckminsterfullerene or its derivatives, nanotubes and derivatives of these materials, and is formed with or from organic material. The electrode substrate is formed through the biomaterial area in electrical isolated manner. The biomaterial area or layers of biomaterial area are formed with or from sequence of mono layers, which are formed as spontaneous self-organizing layer, over second extra charge materials, an inherent surface structure in the material of the electrode substrate and/or over an additional surface material applied on the surface of the electrode substrate of the electrode, and are formed as chemical and/or physically modified or transformed area of the surface of the electrode substrate. The biomaterial area is formed as a layer or with an upper most layer turned away from the electrode substrate with or from an amphiphilen organic connection or the lipid. The area, which is defined through the area of the isolation area isolating and/or covering the electrode has a membrane structure or is formed partially a specific electric conductivity of Gm 1-100 nS/cm2>, a specific capacity of Cm 10-100 nF/cm2> and/or a surface of A 0.1-50 mm2>.