Patent SG-11201804401V-A (SG)
Title: Determination of nucleic acid methylation
Inventor: ZU YANBING
IPC: C12Q1/68
Filed: 2016-11-25
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property ' Organization International Bureau res.. 00) (43) International Publication Date .... ...r .„„*./ 1 June 2017 (01.06.2017) WIPO I PCT (10) WO International 111111111111311111111111111111111111111111111111111111111111111111111311111111111111111 2017/091156 Publication Number Al (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12Q 1/68 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (21) International Application Number: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, PCT/SG2016/050583 DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (22) International Filing Date: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, 25 November 2016 (25.11.2016) KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (25) Filing Language: English OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (26) Publication Language: English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, (30) Priority Data: ZW. 10201509792S 27 November 2015 (27.11.2015) SG (84) Designated States (unless otherwise indicated, for every (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY kind of regional protection available): ARIPO (BW, GH, AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, Connexis, Singapore 138632 (SG). TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (72) Inventors: ZU, Yanbing; do Institute of Bioengineering TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, and Nanotechnology, 31 Biopolis Way, The Nanos, #04- 01, Singapore 138669 (SG). YING, Jackie Y.; c/o Institute LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, of Bioengineering and Nanotechnology, 31 Biopolis Way, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). The Nanos, #04-01, Singapore 138669 (SG). Published: (74) Agent: VIERING, JENTSCHURA & PARTNER LLP; 21(3)) P.O. Box 1088, Rochor Post Office, Rochor Road, Singa- — with international search report (Art pore 911833 (SG). — with sequence listing part of description (Rule 5.2(a)) (54) Title: DETERMINATION OF NUCLEIC ACID METHYLATION Figure 1 \ lix yi s 'Iml kelp D 11 A.mt3lify .. `iEPT., \ Read A co, i Isasst s Lit A Jn AS 6.Ieekee p:pm:Pter PCP Futiuct Various Promega Modified Asymmetric IEN nanoprobe Color signals o commercial MethylEdgeT\" sequences PCR methods Bisulfite according to Conversion references ( - MS methylation-specific) System - 2 5 h t 2. , t mitt V: in 1 Li min IS :. <,‘,.. ‘ CIr.\" If , I :\"' \".: \" ' 3,wkau*nmlis-, 11 os Cr\ i3,:n.st(sft {flati tleikbti.,${ .\.... L ....' (57) : The invention relates to methods and ..::::; sample comprising (a) bisulfite treatment of the target el tion-specific primers, (c) contacting the amplicons Oation status based on melting temperature T„, of the ,-t particle covalently coupled to a morpholino oligonucleotide . kits for determining the methylation nucleic acid, (b) amplifying with a methylation-specific plasmonic hybrid probe and the target probe. Also claimed - the nucleic are methods Mr.;$ 141a/ m, status of a target treated target nucleic nanoprobe and (d) acid. In particular, and kits for nucleic acid molecule in a acid with methyla - deteiinining the methyl - a plasmonic gold nano - determining methylation status of the Septin 9 (SEPT9) gene promoter.