Patent SG-11201807114T-A (SG)
Title: Colony forming medium and use thereof
Inventor: SLUKVIN IGOR
IPC: C12N5/0775
Filed: 2017-03-14
Abstract: MSC AD • MSC BM • iPSC_MSC + iPSC W O 17 / 15 65 80 Al (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/156580 Al 21 September 2017 (21.09.2017) WIPO I PCT (51) International Patent Classification: C12N 5/0775 (2010.01) A61K 35/28 (2015.01) (21) International Application Number: PCT/AU2017/050228 (22) International Filing Date: 14 March 2017 (14.03.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 2016900983 16 March 2016 (16.03.2016) AU 2016904039 5 October 2016 (05.10.2016) AU 2017900318 2 February 2017 (02.02.2017) AU (71) Applicant: CYNATA THERAPEUTICS LIMITED [AU/AU]; Suite 1, 1233 High Street, Armadale, Victoria 3143 (AU). (72) Inventors: SLUKVIN, Igor; 1 Turnwood Circle, Verona, Wisconsin 53593 (US). UENISHI, Gene; 1220 Capitol Ct, Madison, Wisconsin 53715 (US). HEI, Derek; 3221 Tanglewood Dr, Madison, Wisconsin 53719 (US). DRIER, Diana; 302 Glenway Street, Madison, Wisconsin 53705 (US). (74) Agent: GRIFFITH HACK; GPO Box 1285, Melbourne, Victoria 3001 (AU). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) (54) Title: COLONY FORMING MEDIUM AND USE THEREOF PCA: Principal Component Analysis -40 -20 20 40 PG1: 723 Figure 9. (57) : The invention relates to a method for producing a mesenchymal stem cell (MSC), the method comprising culturing a primitive mesoderm cell in a mesenchymal colony forming medium (M-CFM) comprising LiC1 and FGF2, but excluding PDGF, un- der normoxic conditions for sufficient time for a mesenchymal colony to form, and culturing the mesenchymal colony adherently to produce the MSC, wherein the MSC has superior T-cell immunosuppressive properties relative to an MSC not produced in said M- CFM. The invention also relates to an MSC produced by the method, a population of MSCs produced by the method, a therapeutic composition comprising the MSC produced by the method, an M-CFM and an M-CFM in concentrated form, and method and uses of the MSC or population in treating a disease. 111111111111110111011111111111010111110 011111111111111111111101111111111111110111111