Patent WO-2020197370-A2 (WO)
Inventor: NURKENOV TULENDY TULESHEVICH
Filed: 2020-03-02
Abstract: The invention relates to medicine, and more particularly to the creation of a medicinal agent having neuroprotective activity, which can be used for treating acute and chronic ischemic brain injury by restoring brain function in the case of different injuries, and promoting resistance to pathological factors. The aim of the present invention is to provide a plant-based agent having neuroprotective activity, which improves cerebral blood flow and oxygen and glucose supply to the brain, exhibits antihypoxic and antioxidant properties, improves microcirculation, enables improved cognitive function and slows the progression of dementia. The technical result of the proposed agent is that of expanding the line of domestically produced plant-based medicinal agents having neuroprotective activity. This technical result is achieved by using a plant substance extracted from the roots of Siberian statice (Limonium gmelinii) of the genus Limonium Mill of the leadwort family (Plumbaginaceae) as an agent having neuroprotective activity in an intragastric dose of 200 mg/kg. The plant substance extracted from the roots of the Siberian statice plant is comprised of a combination of biologically active substances, including hydrocarbons, complex esters of higher carboxylic acids in the form of hexadecanoic, oleinic and linoleic acid ethyl esters with a predominant presence of polyenoic acid esters, amino acids (all 20 known natural α-amino acids), and sterols (ergosterol, campesterol, stigmasterol, β-sitosterol and campesterol 3-O-β-D-glucopyranoside). The following are identified: flavonols (3,4,5,3',4',6'-hexahydroxyoxyflavone, kaempferol, myricetin, quercetin, isorhamnetin, myricetin monomethyl ether), glycosides thereof (myricitrin, rutin, quercetin 3-β-galactosyl, myricetin 3-β-galactopyranoside, myricetin 3-α-galactopyranoside, myricetin 3-O-α-L-(2"-galloyl)-arabinopyranoside), pyrogallol, gallic and ellagic acid, as well as 3,5,7,3',4',6'-hexahydroxyflavan, (-)-epigallocatechin-(4β→8)-(-)-3,5,7,3',4',6'- hexahydroxyflavan and (+)-gallocatechin-(4α→8)-[(-)- epigallocatechin]5-(4β→8)-(-)- epigallocatechin gallate, and vitamins Е and С. To study the neuroprotective activity of the plant substance extracted from Siberian statice roots using a laboratory animal model, ischemic stroke was induced in male white laboratory rats by middle cerebral artery occlusion (MCAO). This model of ischemic stroke induction in laboratory rats is informative and convenient for studying the pathogenesis of ischemic stroke and for developing methods for the treatment of reperfusion syndrome. The day after induction of ischemic stroke, the animals were administered the plant substance extracted from Siberian statice roots intragastrically in a dose of 200 mg/kg for a period of 28 days. Brain condition was assessed by analyzing motor function using the balance beam method. Data was obtained showing that the plant substance extracted from Siberian statice roots has a comprehensive protective effect on cerebral astrocytes and endotheliocytes. The results of the testing conducted also showed that the plant substance extracted from Siberian statice roots inhibits the development of oxidative stress in neurons. The results of the sensory-motor function assessment in the in vivo model of ischemic stroke showed that the plant substance extracted from Siberian statice roots enables improved sensory-motor function in animals and causes a statistically significant improvement in the motor function of rats with induced ischemic stroke, which demonstrates the neuroprotective activity of the substance of interest. Therefore, on the basis of the experimental data obtained, it can be concluded that the plant substance extracted from Siberian statice roots and administered intragastrically in a dose of 200 mg/kg has neuroprotective activity and that its effect is comparable to that of other neuroprotective agents of plant origin used in medicine.