Patent AU-2015252061-A1 (AU)
Title: Method for Purifying Recombinant ADAMTS13 and Other Proteins and Compositions Thereof
Inventor: MAYER CHRISTA
IPC: C12N9/64
Filed: 2015-11-04
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date H(10) International Publication Number 3 February 2011 (03.02.2011) PCT W O 2011/012726 A2 (51) International Patent Classification: (74) Agents: WIEDEMANN, Peter et al.; HOFFMANN El C12N 9/64 (2006.01) TLE, Arabellastrasse 4, 81925 Munich (DE). (21) International Application Number: (81) Designated States (unless otherwise indicated, for every PCT/EP2010/061192 kind of national protection available): AE, AG, AL, AM, .) . AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, (22) International FingAuust 2010 (02.08.2010) CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, 2 ADZ, EC, EE, EG, ES, Fl, GB, GD, GE, GH, GM, GT, (25) Filing Language: English HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, (26) Publication Language: English ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, (30) Priority Data: NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, 61/230,308 31 July 2009 (31.07.2009) US SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (71) Applicants (for all designated States except US): BAX TER HEALTHCARE S.A. [CH/CH]; Thurgauer Strasse (84) Designated States (unless otherwise indicated, for every 130, CH-8152 Glattpark (Opfikon) (CH). BAXTER IN- kind of regional protection available): ARIPO (BW, GH, TERNATIONAL INC. [US/US]; One Baxter Parkway, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, Deerfield, IL, 60015 (US). ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, (72) Inventors; and EE, ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, (75) Inventors/Applicants (for US only): HASSLACHER, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, Meinhard [AT/AT]; Vorgartenstrasse 221/1/7, A-1020 SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, Vienna (AT). MITTERER, Artur [AT/AT]; GW, ML, MR, NE, SN, TD, TG). Schwarzeckerweg 10, A-2304 Orth/Donau (AT). FIEDLER, Christian [AT/AT]; Raaberbahngasse 18/36, Published: A-1100 Vienna (AT). MAYER, Christa [AT/AT]; Bahn- - without international search report and to be republished hofstrasse 2, A-2412 Wolfsthal (AT). upon receipt of that report (Rule 48.2(g)) (54) Title: METHOD FOR PURIFYING RECOMBINANT ADAMTS13 AND OTHER PROTEINS AND COMPOSITIONS (57) Abstract: Provided herein are methods for purifying recombinant A Disintegrin-Iike and Metallopeptidase with Throm bospondin Type 1 Motif 13 (ADAMTS 13) protein from a sample. The method comprises enriching for ADAMTS 13 protein by chromatographically contacting the sample with hydroxyapatite under conditions that allow ADAMTS 13 protein to appear in the eluate or supernatant from the hydroxylapatite. The methods may further comprise tandem chromatography with a mixed mode f4 cation exchange/hydrophobic interaction resin that binds ADAMTS 13 protein. Additional optional steps involve ultrafiltration/di afiltration, anion exchange chromatography, cation exchange chromatography, and viral inactivation. Also provided herein are methods for inactivating virus contaminants in protein samples, where the protein is immobilized on a support. Also provided herein are compositions of ADAMTS 13 prepared according to said methods.