Patent AU-2012321074-B2 (AU)
Title: Measurement of autoantibodies at low conductivity conditions with increased sensitivity
Inventor: WEBER ALFRED
IPC: G01N33/577
Filed: 2012-12-13
Granted: 2015-12-03
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date W O 2013/090636 Al 20 June 2013 (20.06.2013) W I PO I P CT (51) International Patent Classification: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, G01N33/53 (2006.01) A61K 39/395 (2006.01) DO, DZ, EC, EE, EG, ES, Fl, GB, GD, GE, GH, GM, GT, G01N33/564 (2006.01) G01N33/543 (2006.01) HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, (21) International Application Number: ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, PCT/US2012/069587 NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, (22) International Filing Date: RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, 13 December 2012 (13.12.2012) TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (25) Filing Language: English (84) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of regional protection available): ARIPO (BW, GH, (30) Priority Data: GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, 61/570,211 13 December 2011 (13.12.2011) US UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, (71) Applicants (for all designated States except US): BAX- EE, ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, TER INTERNATIONAL INC. [US/US]; One Baxter MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, Parkway, Deerfield, IL 60015 (US). BAXTER HEALTH- TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, CARE S.A. [CH/CH]; Thurgauerstrasse 130, CH-8152 ML, MR, NE, SN, TD, TG). Glartpark (Opfikon) (CH). Declarations under Rule 4.17: (71) eatsanor US only>: WEBER, Alfred [AT/ AT] as to applicant's entitlement to apply for and be granted a Skraupstrasse 24/42/8, A-1210 Vienna (AT). ENGEL- patent (Rule 4.17(ii MAIER, Andrea [AT/AT]; Benedikt Schellingergasse - as to the applicant's entitlement to claim the priority of the 17/25, A-1150 Vienna (AT). SCHWARZ, Hans-Peter earlier application (Rule 4.17(iii)) [AT/AT]; Weimarer-Strasse 76, A-1 180 Vienna (AT). Published: (74) Agent: PARENT, Annette, S.; Morgan, Lewis & Bockius with international search report (Art. 21(3)) LLP, One Market Spear Street Tower, San Francisco, CA 94105 (US). before the expiration of the time limit for amending the claims and to be republished in the event of receipt of (81) Designated States (unless otherwise indicated, for every amendments (Rule 48.2(h)) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (54) Title: MEASUREMENT OF AUTOANTIBODIES AT LOW CONDUCTIVITY CONDITIONS WITH INCREASED SENS (57) Abstract: Methods for detecting or capturing low-avidity autoantibodies in a biological sample are provided. Target antigen N used to assay for the low-avidity autoantibodies of interest is immobilized on a solid phase. The biological sample is contacted under low conductivity condition with the target antigen for which the autoantibodies has specific binding affinity. Binding of the target antigen to the autoantibodies of interest in the biological sample is then detected to ascertain the presence or concentration of the autoantibodies of interest.