Patent SG-11201407226W-A (SG)
Title: Improved methods of cell culture for adoptive cell therapy
Inventor: VERA JUAN F
IPC: C12N5/0783
Filed: 2013-05-20
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 21 November 2013 (21.11.2013) WIPOIPCT (10) International Publication Number WO 2013/173835 A1 (51) International Patent Classification: C12M 3/02 (2006.01) C12M 3/06 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/US2013/041861 20 May 2013 (20.05.2013) English English (30) Priority Data: 13/475,700 18 May 2012 (18.05.2012) US (71) Applicant: WILSON WOLF MANUFACTURING CORPORATION [US/US]; 33 5th Avenue NW, Suite 700, New Brighton, MN 55112 (US). (72) Inventors: VERA, Juan F.; 5227 Maple Street, Bellaire, TX 77401 (US). ROONEY, Cliona M.; 4802 Willow Street, Bellaire, TX 77401 (US). LEEN, Ann M.; 5227 Maple Street, Bellaire, TX 77401 (US). WILSON, John R.; 173 Windsor Lane, New Brighton, MN 55112 (US). (74) Agents: DICKSON, Thomas G. et al.; Patterson Thuente Pedersen, P.A., 4800 IDS Center, 80 South Eighth Street, Minneapolis, MN 55402-2100 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) (54) Title: IMPROVED METHODS OF CELL CULTURE FOR ADOPTIVE CELL THERAPY m oo m i> Su < o a. w JH INCREASE T ETRAM£R DAY 16 DAY 23 INCREASETETRAMER DAVO DAY 9 DAY 16 DAY 23 TIME IN CULTURE o CJ (57) Abstract: An improved method of culturing cells for cell therapy applications that includes growing desired cells in the pres - ence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cm 2 if the growth surface is not comprised of gas permeable material and up to 2 ml/cm 2 if the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5x10 6 cells/cm 2 at the onset of production cycle, and a the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25 xlO 5 cells/cm . 2