Patent SG-11201810088S-A (SG)
Title: Recovering long-range linkage information from preserved samples
Inventor: TROLL CHRISTOPHER JOHN
IPC: C12N15/10
Filed: 2017-05-12
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER (19) World Intellectual Property 1111111111111111111111111111111111111111 Organization International Bureau (43) International Publication Date .....0\"\" 16 November 2017 (16.11.2017) WIPO I PCT THE PATENT COOPERATION TREATY (PCT) 111111111111111111111111111111111111110111111 (10) International Publication Number WO 2017/197300 Al — (51) (21) (22) (25) Filing Language: (26) (30) (71) (72) (81) International Patent Classification: C12N 15/10 (2006.01) C40B 40/06 (2006.01) C12N 15/11 (2006.01) International Application Number: PCT/US2017/032466 International Filing Date: 12 May 2017 (12.05.2017) English Publication Language: English Priority Data: 62/336,252 13 May 2016 (13.05.2016) US 62/410,599 20 October 2016 (20.10.2016) US Applicant: DOVETAIL GENOMICS LLC [US/US]; 2161 Delaware Ave., Santa Cruz, California 95060 (US). Inventors: TROLL, Christopher John; 63 Rooney Street, Santa Cruz, California 95065 (US). POWERS, Martin P.; 2654 22nd Ave., San Francisco, California 94116 (US). PUTNAM, Nicholas H.; 36 River Street, Apt. 362, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(ii)) — as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) Published: — with international search report (Art. 21(3)) = Waltham, Massachusetts 02453 (US). BLANCHETTE, Marco; 707 Pelton Ave., Apt. 307, Santa Cruz, California 95060 (US). HARTLEY, Paul; 4265 Mountcastle Way, Santa Jose, California 95136 (US). Agent: ANDERSON, Garrett H.; Wilson Sonsini Goodrich & Rosati, 650 Page Mill Road, Palo Alto, Cali- fornia 94304 (US). Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, _ = _ (74) — = = (54) Title: RECOVERING LONG-RANGE LINKAGE INFORMATION FROM PRESERVED SAMPLES = = some processed recover genomic structural information. 101 ` S i C\"\" -......, - ''''''''' :, C . ..., k\,\ , F 1 li : The disclosure provides methods to isolate genome cases, samples preserved under conditions where long-range nucleic acid information is believed to be irreparably lost, such as samples, are treated to recover nucleic acid-protein complexes so as to recover information regarding which nucleic 102 ,-- \. \"\" \ '-'-`1., \ • 103 FP 7\" - • ':ililililil FIG. lA or chromosome level structural information from preserved samples. stabilized as part of the sample preservation process. The complexes acids are bound to a common complex, and the information is used = = = = — 1-1 0 0 M IN 01 1-1 (57) j r:.. .\" In Il FFPE are el to