Patent SG-11201908079S-A (SG)
Title: Recombinant host cell with altered membrane lipid composition
Inventor: GRILLITSCH KARLHEINZ
IPC: C12N15/63
Filed: 2018-03-28
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 04 October 2018 (04.10.2018) W I PO I PCT 011101 V III o Ho VIII VIII om° VII IE (10) International Publication Number WO 2018/178126 Al (51) International Patent Classification: C12N 15/63 (2006.01) Cl 2P 21/00 (2006.01) (21) International Application Number: PCT/EP2018/057853 (22) International Filing Date: 28 March 2018 (28.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 17163588.1 29 March 2017 (29.03.2017) EP (71) Applicants: BOEHRINGER INGELHEIM RCV GMBH & CO KG [AT/AT]; Dr.-Boehringer-Gasse 5-11, 1121 Wien (AT). SANDOZ AG [CH/CH]; Lichtstrasse 35, 4056 Basel (CH). VTU TECHNOLOGY GMBH [AT/AT]; Parkring 18, 8074 Grambach (AT). BIOMIN HOLDING GMBH [AT/AT]; Industriestrasse 21, 3130 Herzogenburg (AT). LONZA LTD [CH/CH]; Lonzas- trasse, 3930 Visp (CH). (72) Inventors: GRILLITSCH, Karlheinz; Buxersiedlung 30, 9470 St. Paul/Lav (AT). DAUM, Guenther; Peterstal- strasse 36b, 8042 Graz (AT). GRUTSCH, Andreas; Hofweg 2, 8046 Stattegg (AT). (74) Agent: WEINZIERL, Gerhard et al.; Schiweck Weinzierl Koch, European Patent Attorneys, Landsberger Strasse 98, 80339 Munich (DE). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, 1-1 EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, 1-1 00 N 1-1 00 1-1 O C TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a)) (54) Title: RECOMBINANT HOST CELL WITH ALTERED MEMBRANE LIPID COMPOSITION (57) : The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.