Patent DE-102007040252-A1 (DE)
Title: Device for electronically flow-controlled distribution of tissue regenerating cell in sterile suspension across an area for further growth comprises electronically pressure/flow controlled spray head enabling controlled pump driven spraying
Inventor: BORNEMANN REINHARD
IPC: B05B12/02
Filed: 2007-08-23
Abstract: A device for electronically flow controlled distribution of tissue regenerating cells including skin cells, in 0.5 - 80 (preferably 5 - 40, especially 5 - 20) ml sterile suspension across an area for further growth, comprises an electronically pressure and/or flow controlled spray head that enables controlled compressed gas and/or pump driven spraying. A device for electronically flow controlled distribution of tissue regenerating cells including skin cells, in 0.5 - 80 (preferably 5 - 40, especially 5 - 20) ml sterile suspension across an area for further growth, comprises an electronically pressure and/or flow controlled spray head that enables controlled compressed gas and/or pump driven spraying. The spray head provides continuous application over 0.5 - 10 (preferably 1 - 5, especially 1 - 2) minutes in a single shot or several shots (preferably up to 3 shots), while generating suspension drops containing cells of size 30 - 500+-200 mm. The spray head is optionally sterilizable or can be covered with a sterile surgery foil hose, where the following procedures are enabled: a) subjecting a tissue sample including cells for grafting onto a patient, to at least a physical and/or chemical dissociating device capable of dissociating cells in the tissue sample; b) taking the cells, which optionally have been expanded after the step (a), for spraying into a saline solution, where the solution is free of xenogenic serum, capable of maintaining the cells alive until applied, contains electrolytes in a physiologic composition, and allows direct application to a biomaterial surface or region on a patient undergoing tissue grafting; c) filtering the cellular suspension produced in steps (a) and (b) to remove cellular conglomerates of more than 10 - 500 (preferably 50 - 300, especially 50 - 100) cells; and d) distributing the cell suspension through the flow controlled spray head onto the recipient surface. Independent claims are included for the following: (1) a cell suspension produced from the device, or from autologous cells including in vitro expanded autologous cells, or from stem cells; (2) utilizing the device and producing a cell suspension for treating a patient requiring graft surgery involving: preparing a cell suspension; and administering the suspension directly to a region on the patient that requires a cell graft in a manner that facilitates spraying of the cell suspension in an even distribution over the graft area; and (3) coating an artificial surface or a biomaterial surface for commercial use with the device involving: preparing a cell suspension; and administering the suspension directly onto an artificial or a biomaterial that requires a cell coated surface in a manner that facilitates spraying of the cell suspension in an even distribution. ACTIVITY : Dermatological; Vulnerary. No biological data is given. MECHANISM OF ACTION : None given.