Patent SG-11201811600P-A (SG)
Title: Pseudotyped oncolytic viral delivery of therapeutic polypeptides
Inventor: EVNIN LUKE
IPC: A61K39/395
Filed: 2017-06-30
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) -., (19) World Intellectual Property 11111111111111111111111111111111111111111111111111111111111111111111111110111111 Organization International Bureau (10) International Publication Number 03 (43) International Publication Date .....•\"\" WO 2018/006005 Al 04 January 2018 (04.01.2018) WIP0 I PCT (51) International Patent Classification: HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, A61K 39/395 (2006.01) A61K 35/76 (2006.01) KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (21) International Application Number: MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, PCT/US2017/040354 OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, (22) International Filing Date: TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. 30 June 2017 (30.06.2017) (84) Designated States (unless otherwise indicated, for every (25) Filing Language: English kind of regional protection available): ARIPO (BW, GH, (26) Publication Language: English GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (30) Priority Data: TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, 62/357,195 30 June 2016 (30.06.2016) US EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (71) Applicant: ONCORUS, INC. [US/US]; 450 Kendall TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, Street, 5th Floor, Cambridge, Massachusetts 02142 (US). KM, ML, MR, NE, SN, TD, TG). (72) Inventor: EVNIN, Luke; c/o Oncorus, Inc., 450 Kendall Street, 5th Floor, Cambridge, Massachusetts 02142 (US). Published: — with international search report (Art. 21(3)) (74) Agent: PHELAN, Jordan D. et al.; Cooley LLP, 1299 — before the expiration of the time limit for amending the Pennsylvania Avenue, N.W., Suite 700, Washington, Dis- claims and to be republished in the event of receipt of trict of Columbia 20004 (US). amendments (Rule 48.2(h)) = (81) Designated States (unless otherwise indicated, for every — with sequence listing part of description (Rule 5.2(a)) kind of national protection available): AE, AG, AL, AM, =_ AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (54) Title: PSEUDOTYPED ONCOLYTIC VIRAL DELIVERY OF THERAPEUTIC POLYPEPTIDES = = FIG. 1 = CD19-CD3 BiTE (SEQ ID NO: 44) = _ ,,, si': — .M. , .. , .;;;: , .. ,, eseT.i.VAI. V •• .4-7t • • AV L'r)•Y r) , S11\". '.• . N : V = — : G..:'• ,.. - • • • ..'DAA = .‘'‘ZA - IMER Heavy c• -, ,, Mil = = = = \" '. :, •,... q.i.i , i ••[ :.; i ' , .1' - 11 %.. ..: ..: .',.• i.:: , W ffeammu . - ....„ kr) e.. D AA' i': i':i.:i.:i.:''' . © Signal peptide 1: SEQ ID NO: 2 Light chain CD3: SEQ ID NO: 20 IX G4S linker: SEQ ID NO: 6 © Light chain CD19: SEQ ID NO: 16 Hea%y chain CD3: SEQ ID NO: 22 G2S linker: SEQ ID NO: 10 © Heavy chain CD19: SEQ ID NO: 18 3X G4S linker: SEQ ID NO: 8 His-tag: SEQ ID NO: 12 ---.... 1 3C C (57) : Described herein are pseudotyped oncolytic viruses comprising nucleic acids encoding an engager molecule. In some \" embodiments, the pseudotyped oncolytic viruses comprises nucleic acids encoding an engager molecule and one or more therapeutic molecules. Pharmaceutical compositions containing the pseudotyped oncolytic virus and methods of treating cancer using the pseudo- 0 typed oncolytic viruses are further provided herein.