Patent AU-2012323042-B2 (AU)
Title: Plants with decreased activity of a starch dephosphorylating enzyme
Inventor: ZEEMAN SAMUEL C
IPC: C12N15/82
Filed: 2012-10-10
Granted: 2015-01-15
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date W O 2013/053729 Al 18 April 2013 (18.04.2013) W I PO I P CT (51) International Patent Classification: NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, C12N15/82 (2006.01) C12N 9/16 (2006.01) RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, (21) International Application Number: ZM, ZW. PCT/EP2012/070017 (22) International Filing Date: (84) Designated States (unless otherwise indicated, for every (22) International Filing Da2 kind of regional protection available): ARIPO (BW, GH, 10 October 2012 (10.10.2012) GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, (25) Filing Language: English UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, (26) Publication Language: English EE, ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (30) Priority Data: MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, 11184945.1 12 October 2011 (12.10.2011) EP TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, 61/546,228 12 October 2011 (12.10.2011) US ML, MR, NE, SN, TD, TG). (71) Applicant: BAYER CROPSCIENCE AG [DE/DE]; Al- Declarations under Rule 4.17: fred-Nobel-Strasse 50, 40789 Monheim am Rhein (DE). - as to the identity of the inventor (Rule 4.17(i)) (72) Inventors: ZEEMAN, Samuel C.; Hammerstrasse 54C, - as to applicant's entitlement to applyfor and be granted a CH-8032 Zurich (CH). KOTTING, Oliver; Ritterstrasse patent (Rule 4.17(ii)) 5, CH-8032 Zurich (CH). SANTELIA, Diana; Guldenen- - as to the applicant's entitlement to claim the priority of the strasse 39B, CH-8610 Uster (CH). earlier application (Rule 4.17(iii)) (74) Agent: HENNING-MIXA, Karen; Technologiepark 38, - of inventorship (Rule 4.17(iv)) B-9052 Gent (BE). Published: (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, - with international search report (Art. 21(3)) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, - before the expiration of the time limit for amending the BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, claims and to be republished in the event of receipt of DO, DZ, EC, EE, EG, ES, Fl, GB, GD, GE, GH, GM, GT, amendments (Rule 48.2(h)) HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, with sequence listing part of description (Rule 5.2(a)) ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, (54) Title: PLANTS WITH DECREASED ACTIVITY OF A STARCH DEPHOSPHORYLATING ENZYME (57) Abstract: The present invention relates to plant cells and plants that are genetically modified, whereby the genetic modification leads to a decreasein the activity of a starch dephosphorylating LSF-2 proteinand a starch dephosphorylating SEX4 proteinin com parison to corresponding wild type plant cells or wild type plants that have not been genetically modified. The present invention also relates to means and methods for the manufacture of such plant cells and plants. These types of plant cells and plants synthesise a modified starch. Therefore, the present invention also concerns the starch synthesised from the plant cells and plants according to the f4 invention, methods for the manufacture of this starch, and the manufacture of starch derivatives of this modified starch, as well as flours containing starches according to the invention. In addition, the present invention relates to vectorscomprising nucleic acids encoding a starch dephosphorylating LSF-2 proteinstarch dephosphorylating SEX4 protein, host cells such asplant cells, and plants containing such chimeric genes.