Patent AU-2020103945-A4 (AU)
Title: EPBP- Euphorbia Milii: Evaluation of Phytoconstituents and Bioactivity Potential of Euphorbia Milii des Moul Aerial Parts
Inventor: CH PRADYUTHA A
IPC: A01N65/18
Filed: 2020-12-08
Granted: 2021-02-18
Abstract: Our Invention" EPBP- Euphorbia Milii" is an aerial part (leaf, stem and flower) of the 
E. milii were extracted using hexane, chloroform, acetone, ethyl acetate, ethyl alcohol, 
methanol and aqueous solvents. From the phytochemical studies of the various 
solvent extracts of leaf, stem and flower parts of E. milii, it was found that leaves and 
flower extracts are predominant in the presence of all the secondary metabolites 
tested followed by stem extracts. It is reflecting the same in terms of antimicrobial 
activity. The screening of the biological activity of all the solvent extracts of leaf, stem 
and flower included the determination of antimicrobial activity using agar well 
diffusion method. It is proving that, there is a significant relation between the 
presence of secondary metabolites in the plant parts and their antimicrobial activity 
against the test cultures employed. The Minimum Inhibitory Concentration (MIC) 
and Minimum bactericidal concentration (MBC) of the positive solvent extracts were 
estimated to know the efficiency of an antibacterial agent. The value of MIC was found 
to be in the range of 12.5 to 75 mg/ml for the positive leaf, stem and flower extracts 
and MBC was in the range of 25mg/ml to 100 mg/ml and the leaf, stem and flower 
extracts of E. milii plant also showed significant antifungal activity against plant 
pathogens. Ethyl alcohol extracts of leaf showed maximum activity against all the 
tested fungi i.e., Fusarium oxysporum, Sclerotium hydrophilum, Alternaria sps than 
the standard chemical fungicide flucanazole. The methanol and aqueous extracts of 
stem and ethyl alcohol, methanol and aqueous extracts of flower was found efficient, 
exhibited inhibitory activity against all the tested fungi. The value of MIC was found 
to be in the range of 6.25 to 25 mg/ml for the positive leaf and stem extracts and the 
MIC value of flower was in the range of 12.5mg/ml to 50mg/ml and the MFC Values of 
the leaf and stem solvent extracts ranges between 12.5 to 50 mg/ml and the MFC 
values of flower was found in the range of 12.5 to 75mg/ml. Collection and drying of 
the stem, leaf and flowers of Euphorbia milli plant and Preparation of crude extracts 
from stem, leaves and flowers of the plant in different solvents viz., Hexane, 
Chloroform, Ethyl acetate, Acetone, Methanol, Ethanol and Water in their increasing 
order of polarity. Synthesis of silver nanoparticles using Leaf, Stem and Flower 
extracts and characterization of synthesized nanoparticles by UV, FTIR, TEM and SEM 
analyses and checking the antimicrobial activity of synthesized silver nanoparticles.  
The invented methods also provide the purification of the active compounds present 
in the potential solvent extracts of leaves, stem and flowers with good antimicrobial 
activity by Column Chromatography and characterization of pure compounds from 
the active fractions showing good antimicrobial activity by 1NMR, 13 C NMR and Mass 
spectrometry analyses. Examination of antimicrobial and cytotoxic activities of the 
isolated pure compounds and a new distinct cultivar of Euphorbia milii plant named 
'Zeus', particularly characterized by its compact plant habit. The invented methods 
also provide the very low and bushy plant form vigorous growth habit, brown 
streaked stems, abundant, large floral cymes with red bracts from 45C to bright deep 
red 50A, and more flowers on thick, stiff gray-brown peduncles N199D.  
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FIG.1: EUPHORBIA MILIIAND COLOR-R