Patent GB-682768-A (GB)
Title: Fractionation and purification of biological solutions by ion-exchange
IPC: C07K16/06
Filed: 1949-04-12
Abstract: A separation of certain proteins from other proteins in a mixed protein containing solution also containing natural anion and cation solubilizing ions, the said certain proteins being solubilized by said ions and the said other proteins being soluble whether or not the said ions are present, is obtained by the removal of said ions by passing said solution through a mixture of an anion exchange resin and a cation exchange resin, which mixture is conditioned to be in equilibrium with the ion concentration desired.  Thus in the case of human blood plasma, the globulins (medicinally useful as e.g. immune sera) are separated from the albumins (suitable for treatment of shock) by percolation through a column of a mixed ion exchange resin, e.g. 4 parts Amberlite IR100H (cation-exchange resin) and 9 parts "Deacidite" (Registered Trade Mark) (anion-exchange resin).  The sodium chloride present in the plasma is removed and the globulins precipitated on the column.  The resin mixture is such that the NaCl is removed without change in pH.  The column is washed with distilled water to remove entrained albumins, and the globulins eluted therefrom with aqueous sodium chloride.  If desired, the globulins may be fractionated by elution with saline solutions of controlled pH.  The albumin solution may be purified by passage through a bed of the cation exchange resin conditions with distilled water, and then through one conditioned with pyrogen-free water.  Finally, the solution may be passed through a bacterial filter.  Further examples illustrate (a) the removal of haemoglobin from blood serum, (b) the separation of milk proteins and (c) the resin artificial kidney.ALSO:A separation of certain proteins from other proteins in a mixed protein containing solution also containing natural anion and cation solubilising ions, the said certain proteins being solubilised by said ions and the said other proteins being soluble whether or not the said ions are present, is obtained by the removal of said ions by passing said solution through a mixture of an anion exchange resin and a cation exchange resin, which mixture is conditioned to be in equilibrium with the ion concentration desired.  Thus in the case of human blood plasma, the globulins (medicinally useful as e.g. immune sera) are separated from the albumins (suitable for the treatment of shock) by percolation through a mixed ion exchange resin column e.g. 4 parts Amberlite 1R 100 H (cation exchange resin) and 9 parts "Deacidite" (anion exchange resin) (R.T.M.).  The sodium chloride present in the plasma is removed and the globulins are precipitated on the column.  The resin mixture is such that the NaCl is removed without change in pH.  The column is washed with distilled water to remove entrained albumins, and the globulins eluted therefrom with sodium chloride solution.  If desired the globulins may be fractionated by elution with saline solution at a controlled pH.  The albumin solution may be purified by passage through a bed of the cation exchange resin conditioned with distilled water, and then through one conditioned with pyrogen-free water.  Finally the solution may be passed through a bacterial filter.  Further examples illustrate (a) the removal of h moglobin from blood serum (b) the separation of milk protein and (c) the resin artificial kidney.