Patent GB-612002-A (GB)
Title: Improvements in medicinal antipressors and methods of making them
IPC: A61K38/00
Filed: 1946-06-14
Abstract: A medicinal antipressor is prepared by the interaction of hippuric acid and urobilinogen or an extract containing urobilinogen. According to an example, urine of horses or other liver-bearing animals is agitated with butyl ether for several hours.  After standing two layers separate, the upper butyl ether layer containing fat &c. being discarded while the lower aqueous layers is slowly evaporated at a temperature not above 170 DEG  F. to reduce its volume.  The aqueous layer is then slightly acidified p  with acetic acid and the evaporation is continued to produce a semi-fluid black mass.  The black mass is extracted with ethyl acetate, the extract being filtered and evaporated to dryness. The residue is dissolved in a mixture of alcohol and chloroform and the solution after filtering is mixed with distilled water. The resulting lower chloroform layer is evaporated to dryness to produce a urobilinogen concentrate.  The concentrate is mixed with hot water and hippuric acid, the mixture being heated until all the urobilinogen concentrate dissolves.  The hot solution is filtered and on cooling, crystals of the antipressor composition separate.  The Specification as open to inspection under Sect. 91 comprises also the preparation of an antipressor composition by reacting benzoic acid with urobilinogen or an extract containing urobilinogen.  This subject-matter does not appear in the Specification as accepted.ALSO:A medicinal anti-pressor composition is prepared by the interaction of hippuric acid and urobilinogen or an extract containing urobilinogen.  According to an example urine of horses or other liverbearing animals is agitated with butyl ether for several hours.  After standing two layers separate, the upper butyl ether layer containing fat &c. being discarded while the lower aqueous layer is slowly evaporated at a temperature not above 170 DEG F. to reduce its volume.  The aqueous layer is then slightly acidified with acetic acid and the evaporation is continued to produce a semi-fluid black mass.  The black mass is extracted with ethyl acetate, the extract being filtered and evaporated to dryness, the residue is dissolved in a mixture of alcohol and chloroform and the solution after filtering is mixed with distilled water.  The resulting lower chloroform layer is evaporated to dryness to produce a urobilinogen concentrate.  The concentrate is mixed with hot water and hippuric acid, the mixture being heated until all the urobilinogen concentrate dissolves.  The hot solution is filtered and on cooling crystals of the anti-pressor composition separate.  For injection the composition may be dissolved in a solution of sodium bicarbonate, the solution being slightly warmed to eliminate generated carbon dioxide; it may also be administered orally in the dry form.  The Specification as open to inspection under Sect. 91 comprises also the preparation of an anti-pressor composition by reacting benzoic acid with urobilinogen or an extract containing urobilinogen.  This subject-matter does not appear in the Specification as accepted.