Patent SG-11201809329P-A (SG)
Title: Cell culture
Inventor: KHOO BEE LUAN
IPC: C12N5/095
Filed: 2016-04-29
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property : 7 --- - ` , Organization i 111111101111 0 DOI 010111110101111 0 ICI Millin0110111110111101111 International Bureau (10) International Publication Number (43) International Publication Date .....•\"\" WO 2017/188890 Al 02 November 2017 (02.11.2017) W I PO I PCT (51) International Patent Classification: Mechanobiology Institute, 21 Lower Kent Ridge Road, Sin- C12N 5/095 (2010.01) gapore 119077 (SG). (21) International Application Number: (74) Agent: AMICA LAW LLC; 30 Raffles Place, #14-01 PCT/SG2016/050197 Chevron House, Singapore 048622 (SG). (22) International Filing Date: (81) Designated States (unless otherwise indicated, for every 29 April 2016 (29.04.2016) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (25) Filing Language: English CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, (26) Publication Language: English EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, (71) Applicant: NATIONAL UNIVERSITY OF SINGA- LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, PORE [SG/SG]; 21 Lower Kent Ridge Road, Singapore MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, 119077 (SG). PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, (72) Inventors: KHOO, Bee Luan; c/o National University of SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, Singapore, Mechanobiology Institute, 21 Lower Kent Ridge UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Road, Singapore 119077 (SG). LIM, Chwee Teck; c/o (84) Designated States (unless otherwise indicated, for every National University of Singapore, Mechanobiology Insti- kind of regional protection available): ARIPO (BW, GH, tote, 21 Lower Kent Ridge Road, Singapore 119077 (SG). GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, GRENCI, Gianluca; c/o National University of Singapore, — Title: CELL CULTURE = (54) = Figure 2 = A = Gradient Generator 7 h ‘ = — 1 1 = — \ Barrier = = = = = ‘ \ = — _ = , k \ Microwell --,- Il © t:t GC (57) : The disclosure relates to the enrichment and expansion of rare cells in blood such as circulating tumor cells (CTCs), GC cancer stem cells [CSCs] and other rare circulating cells. The microwells promote interactions between patient-derived CTCs and blood GC cells, allowing expansion of CTCs without the need for pre-enrichment or additional growth supplements. The cultured cells can be Il ----- selected for propagation from single cells and have utility in drug screening, diagnostics and prognostics. The disclosure also includes a IN ,_ 1 system comprising a cell enrichment device for enriching CTCs and CSCs and a device adapted to co-operate with the cell enrichment © device to allow the testing of one or more agents, for example therapeutic or diagnostic agents. N o [Continued on next page] WO 2017/188890 Al MIDEDIMOMOIDEIROIDEMOMMHHEIMOIHMEMOIMIE UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3))