Patent SG-11201908359U-A (SG)
Title: Neuromuscular junction: nmj-on-chip
Inventor: KERNS S JORDAN
IPC: B01L3/00
Filed: 2018-03-14
Abstract: rigIC irly,S - actin M r Myofi - 6 Pig. 113 Axon of k•. nerve YrbeY ttlitoehondm Fig. 1A sarcoplasmic reticulum ?anew nem csaiealltdiVAGh) activation pokalial co, usele cell membrane activation potential (AP) Mza 1-1 1-1 O N 1-1 00 O N C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 20 September 2018 (20.09.2018) WIP0 I PCT omit °nolo olo mo !ammo iflo oimIE (10) International Publication Number WO 2018/170139 Al (51) International Patent Classification: BOIL 3/00 (2006.01) C12N 5/0793 (2010.01) C12N 5/077(2010.01) (21) International Application Number: PCT/US2018/022455 (22) International Filing Date: 14 March 2018 (14.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 15/458,185 14 March 2017 (14.03.2017) US (71) Applicants: EMULATE, INC. [US/US]; 27 Drydock, Boston, MA 02210 (US). CEDARS-SINAI MEDICAL CENTER [US/US]; 8700 Beverly Blvd., Los Angeles, CA 90048 (US). (72) Inventors: KERNS, S. Jordan; 7 Heritage Dr., Read- ing, MA 01867 (US). WEN, Norman; 4975 Washington Street, Unit 118, West Roxbury, MA 02132 (US). HAMIL- TON, Geraldine; 509 East 2nd Street, Unit #7, Boston, MA 02127 (US). HINOJOSA, Christopher David; 69 Holwor- thy Street, Cambridge, MA 02138 (US). FRASER, Jacob; 19 Kingston St Apt 2, Somerville, MA 02144 (US). KAR- ALIS, Catherine; 9 Colbourn Crescent #2, Brookline, MA 02445 (US). SAREEN, Dhruv; 19375 Crystal Ridge Lane, Porter Ranch, CA 91326 (US). KAUS, Anjoscha; 107 S. Croft Avenue, Los Angeles, CA 90048 (US). MANDE- FRO, Berhan; 14640 Burbank Blvd. #215, Sherman Oaks, CA 91411 (US). PARK, Hyoung Shin; 42 Summit St, Newton, MA 02458 (US). KUJALA, Ville; 11 Strathmore Road #6, Medford, MA 02155 (US). (74) Agent: CARROLL, Peter G. et al.; Medlen & Carroll, LLP, 1440 Broadway, Suite 1010, Oakland, CA 94612 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (54) Title: NEUROMUSCULAR JUNCTION: NMJ-ON-CHIP Pig. IA-C (57) : The invention relates to culturing motor neuron cells together with skeletal muscle cells in a fluidic device under conditions whereby the interaction of these cells mimic the structure and function of the neuromuscular junction (NMJ) providing a NMJ- on-chip. Good viability, formation of myo-fibers and function of skeletal muscle cells on fluidic chips allow for measurements of muscle cell contractions. Embodiments of motor neurons co-cultures with contractile myo-fibers are contemplated for use with modeling diseases affecting NMJ's, e.g. Amyotrophic lateral sclerosis (ALS). [Continued on next page] WO 2018/170139 Al MIDEDIMOMOIDEIREEMOMMEIROMMIDEVOIMIE DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h))