Patent SG-11201906480Q-A (SG)
Title: Engineered glycosyltransferases and steviol glycoside glucosylation methods
Inventor: VROOM JONATHAN
IPC: C12N9/10
Filed: 2018-02-01
Abstract: OHNDP-glucose Acceptor Accept2113-giucoside GT R—OH HO OR 0 OH P OH —Nucleotide R'—OH Syathase Byproduct HO O HO HO OH OH —0 O OH OH (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 09 August 2018 (09.08.2018) WIP0 I PCT omit °nolo olo Ho ioo (10) International Publication Number WO 2018/144679 A3 (51) International Patent Classification: C12P 19/56 (2006.01) Cl 2N 9/10 (2006.01) (21) International Application Number: PCT/US2018/016359 (22) International Filing Date: 01 February 2018 (01.02.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/454,417 03 February 2017 (03.02.2017) US 62/479,262 30 March 2017 (30.03.2017) US (71) Applicant: CODEXIS, INC. [US/US]; 200 Penobscot Dri- ve, Redwood City, California 94063 (US). (72) Inventors: VROOM, Jonathan; 200 Penobscot Dri- ve, Redwood City, California 94063 (US). GALANIE, Stephanie, Sue; 200 Penobscot Drive, Redwood City, Cali- fornia 94063 (US). DELLAS, Nikki; 200 Penobscot Drive, Redwood City, California 94063 (US). LIANG, Jack; 200 Penobscot Drive, Redwood City, California 94063 (US). LIU, Joyce; 200 Penobscot Drive, Redwood City, Cali- fornia 94063 (US). ENTWISTLE, David; 200 Penobscot Drive, Redwood City, California 94063 (US). MOFFETT, Courtney, Dianne; 555 4th Street, Unit 615, San Francisco, California 94107 (US). (74) Agent: MACKNIGHT, Kamrin, T.; Codexis, Inc., 200 Penobscot Drive, Redwood City, California 94063 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (54) Title: ENGINEERED GLYCOSYLTRANSFERASES AND STEVIOL GLYCOSIDE GLUCOSYLATION METHODS OH OH Glucose donor FIG. 1 (57) : The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encod- ing these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with 0-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided. [Continued on next page] WO 2018/144679 A3 IMEDIMOM0101011MENOMMHOIEINEHOMEEN DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a)) (88) Date of publication of the international search report: 13 December 2018 (13.12.2018)