Patent SG-11201408138X-A (SG)
Title: Method for producing a collagen membrane and uses thereof
Inventor: ZHENG MING-HAO
IPC: A61L27/24
Filed: 2013-06-12
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 19 December 2013 (19.12.2013) WIPOIPCT (10) International Publication Number WO 2013/185173 A1 (51) International Patent Classification: A61L 27/24 (2006.01) A61F2/18 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/AU2013/000621 12 June 2013 (12.06.2013) English English (30) Priority Data: 2012902458 12 June 2012 (12.06.2012) (71) Applicant: THE UNIVERSITY OF WESTERN AUS­ TRALIA [AU/AU]; Nedlands, Western Australia 6907 (AU). (72) Inventor: ZHENG, Ming-Hao; 2 Kingsland Avenue, City Beach, Western Australia 6015 (AU). (74) Agent: GRIFFITH HACK; Level 19, 109 St Georges Terrace, Perth, Western Australia 6000 (AU). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Au (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). [Continued on next page] (54) Title: METHOD FOR PRODUCING A COLLAGEN MEMBRANE AND USES THEREOF l> i-H oo i-H o CJ o & Figure 1 (57) Abstract: The present invention relates to a method of producing a col­ lagen membrane that has particular mechanical properties. In particular, the present invention relates to a method A of producing a collagen membrane comprising the steps of (i) isolating collagen-containing a tissue and incub - ating same in an ethanol solution; (ii) incubating the collagen-containing tis - sue from step (i) in a first solution comprising an inorganic salt and an an­ ionic surfactant in order to denature non-collagenous proteins contained therein; (iii) incubating the collagen- containing tissue produced in step (ii) in second solution comprising a an inorganic acid until the collagen in said material is denatured; and (iv) incubating the collagen-containing tissue pro­ duced in step (iii) in a third solution comprising an inorganic acid with sim­ ultaneous mechanical stimulation for sufficient time to enable the collagen bundles in said collagen- containing tissue to align; wherein the mechanical stimulation comprises applying tension cyclically to the collagen-containing tissue. WO 2013/185173 A11 lllll llllllll II llllll III lllll lllll III III III lllll lllll lllll lllll llll lllll llll lllllll llll llll Published: — with international search report (Art. 21(3))