Patent SG-11201406876V-A (SG)
Title: Novel d-lactic acid-producing strain and use thereof
Inventor: YANG EUN BIN
IPC: C12N1/21
Filed: 2013-04-24
Abstract: (43) ^n^7B Q 2013 \£ 10 ^ 31 H (31.10.2013) W1POIPCT (10) ^1^7111*1 Jl WO 2013/162274 A1 (51) ^1^^: C12N1/21 (2006.01) (2i) (22) (25) (26) ^7floi<H : (30) PCT/KR2013/003501 2013 4 € 24 H (24.04.2013) ^o] 10-2012-0042894 2012 4 € 24 H (24.04.2012) KR (71) *1 *ll °1 *i| 1M1 # (^r) (CJ CHEILJEDANG CORPORATION) [KR/KR]; 100-400 ^1^1 V>S- 3. 330, Seoul (KR). (72) \"3=^:^1 (YANG, Eun Bin); 138-220 A] vr A] -£• ^ 5 529 ^ 902 S., Seoul (KR). TT, TZ, (LEE, TaeHee); 463-020 ^7] £ ¥ °eW a- 602 ^ 1008 J:, Gyeonggi-do (KR). Q -S3I (KIM, Seon Hye); 422-040 ^7] £ -7- l A 102 104 J:, Gyeong­ gi-do (KR). (YANG, Young Lyeol); 412-220 ^ 7]SL JI^^I i3i <3=^ S^A]S 1910 ioi J:, Gyeonggi-do (KR). 2}-§-\"S (LI, Hong Xian); 157-200 A| ^Al 7j-A^ 7}<&=- 92-1, Seoul (KR). (74) ^ (SON, Min); 135-973 A^A! £3.87^ 36,6# \"oWfl), Seoul (KR). (81) *13^ (l^ 2 ! i A] 7} ^ ?V,7}#?!: JSL-s- ^1 e] S\ iL J:# ^ t} ): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) X|^(!;£°] iAl7} &ir ?V,7}#?!: 5L& #fr°] « ifl ^1 e] S\ iL J:# ^ ): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), -rr A 1 o} (AM, AZ, BY, KG, KZ, RU, TJ, TM), -rr v! (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, [Qi n* (54) Title: NOVEL STRAIN PRODUCING D-LACTIC ACID AND (54)1-^31 ^ Dij SI 321 Dl-LA • D-LA 12 10 -)• USE THEREOF (57) Abstract: The present invention relates to a method for producing mutated strain which produces a D-lactic acid, to a mutated strain producing D-lactic acid which is produced by the method, and to a method for producing D-lactic acid comprising the steps of culturing the strain and obtaining D- lactic acid from the culture, wherein the method for produ­ cing the mutated strain comprises the steps of: inhibiting the activity of L-lactic acid dehydrogenase (L-LDH) in a strain producing L-lactic acid; and introducing D-lactic acid de­ hydrogenase (D-LDH) activity. Since the D-lactic acid pro­ ducing strain of the present invention is produced by mutat­ ing a strain having excellent L-lactic acid producibility to have excellent D-lactic acid producibility, the present inven­ tion can be widely applied to increasing the producibility of various articles which use D-lactic acid as a raw material. (57) £ t'lfLl L«j ^5 4 Tf^rii^L-LDH)^ *ll JL, D «j ^A> ii(D-LDH)^ lt^- •t l> CJ CJ i-H cn i-H o CJ o & AA.. BB .. CC .. DD.. EE . FF. GG. HH . . II. JJ . . Lb. Lb. pentosus Lb. rhamnosus Lb. jensenii Lb. plantarum . Lb. fermentum . Lb. paracasei Lb. . Lb. Lb. brevis K:9 acidophilus johnsonii casei >a o ]SlDf ^A> Aj-Tl 0_ S- 7112:51 lgo]5l D ^A> ^A>5^ ^ ^ <$7] ^ # T3fl <& §|-JI tifl'Sl-S-SfBl D«j ^A>-i: ^7]] # D«j ^A>^ 7112:^1^011 7^0} V} . a. D«j ^A> L«j ^A> ^A>^^1 -f=r & ^JWft ^ l 0 A l7l 7ll^sl ^o_SAi,D«j # 7llf-°l AJXj-^g^AT-Al^lfecll i^el !-§-€ ^ §1-1: WO 2013/162274 AI I lllll llllllll II llllll III lllll lllll III III III lllll lllll lllll lllll lllll llll llll lllllll llll llll ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). — ^ JL *\ 4 (2: <$ 21 2:(3)) 7l?V ^-T- (i? ! 4 48.2(h)) Ai<g-E-S Jf ^-^11 (fl-z] 5.2(a))