Patent FR-2891278-A1 (FR)
Title: In vitro evaluation of procaryotic/eucaryotic cell collection having an intracellular dysfunction, by a procaryotic/eucaryotic cell collection determining system using a set of biological markers
Inventor: REISS CLAUDE
IPC: G16B50/30
Filed: 2005-09-23
Abstract: The in vitro evaluation of procaryotic/eucaryotic cell collection having a intracellular dysfunction, by a procaryotic/eucaryotic cell collection determining system using a set of biological markers, comprises initializing the system, starting the system by selecting the marker, adding an associated data to the selected marker in the storage unit and selecting the resource unit to carry out a test of the biological marker state and to transmit an execution command to the evaluation unit, recovering the test results carried out by selecting the marker, and analyzing the test results. The in vitro evaluation of procaryotic/eucaryotic cell collection having a intracellular dysfunction, by a procaryotic/eucaryotic cell collection determining system using a set of biological markers, comprises initializing the system, starting the system by selecting the marker, adding an associated data to the selected marker in the storage unit and selecting the resource unit to carry out a test of the biological marker state and to transmit an execution command to the evaluation unit, recovering the test results carried out by selecting the marker, and analyzing the test results. The system is initialized by charging the data storage unit, informations and instructions of the storage unit concerning the calculation of priority values, and the initial information in the operational planning unit. The test results are carried out by charging the state parameters generated by the resource unit, and transmitting the charged state parameters towards the storage unit and the analyzing unit. The test results are analyzed by calculating a priority value that defines the relation of the biological markers using the stored function in the storage unit, for each biological marker for which the state parameter is transmitted by the analyzing unit, and propagating the calculated priority value. The resource unit comprises a DNA chip and a protein chip. The system comprises data storage units (10, 20) to characterize a number of biological markers and a metric relation between the biological markers, a set of resource unit, an evaluation unit, a storage unit, an analyzing unit, an operational planning unit, an initialization unit and a control unit (100). The data storage unit comprises identification reference units of the biological markers in which state parameters are determined by the resource unit, and a state parameter value, which defines a test result of the biological marker carried out by the resource unit. The set of resource unit has a function to transmit the state parameters of the biological markers contained or expressed by a cell culture, towards the evaluation unit of the state parameters. The resource unit (60) comprises an identification unit, an indication unit, a determination unit and a transmission unit in which a signal is transmitted between the resource unit and the evaluation unit of the state parameters. The evaluation unit is used for the execution of tests and result storage of tests carried out by resource unit. The state parameters are generated by the resource unit. The storage unit and the analyzing unit are used for the calculation of a priority value of the biological marker using a stored function in the unit. The operational planning unit comprises a set of data for the execution of tests for the biological markers, and a unit for initial configuration of the priority data for the each marker in which the priority data is positive or null. The priority value defines the priority row of the biological markers. The initialization unit comprises a transmission unit in which the signal is transmitted between the initialization unit and the control unit. The control unit comprises a transmission unit in which the signal is transmitted between the control unit and the resource unit, and commanding units for the operation of resource units. The biological markers comprise markers indicating the presence/absence of a nucleic acid in a genome of the tested cells, the presence/absence of polymorphic sites in the sequence of a genomic nucleic acid of the tested cells, the presence/absence of a specific allele in a polymorphic site contents in the genomic nucleic acid, the presence/absence of a transcription product of a gene contained in the genome comprising a RNA messenger or a complementary DNA, the transcription level of a gene contained in the genome, the presence/absence of a protein coded by the genome or by an organism lodged in a virus/fungi, a translation level of a protein coded by the genome or by the organism, an intracellular/extracellular quantity of a protein coded by the genome or by the organism and the presence/absence/quantity of any other detectable cellular metabolite comprising cofactors of enzymes, intracellular/extracellular receptors, lipids, fatty acids, polyosides, vitamins or trace elements. The priority value is calculated from the cell collection and the execution of the resource unit configured with the marker and a sample derived from the cell collection. The process is used to carry out multiple evaluations of arbitrary substances on arbitrary cell cultures under concentration and arbitrary time of exposure with the biological markers. An independent claim is included for a system for determining procaryotic/eucaryotic cell collection using a set of biological markers.