Patent DE-102009052404-A1 (DE)
Title: System for high throughput screening of candidate compounds, comprises a first and second type of lipid membrane vesicle, and a device for the determination of desired effect of the candidate compounds to be tested
Inventor: ZEILINGER CARSTEN
IPC: C12Q1/02
Filed: 2009-11-10
Abstract: The system for high throughput screening of candidate compounds, comprises a first type of lipid membrane vesicle A containing a determined quantity of functional channel protein complex in the lipid membrane and containing a reaction partner I in a medium in internal volume of the lipid membrane vesicle A, and a second type of lipid membrane vesicle B containing functional channel protein complex in the lipid membrane and containing a reaction partner II in internal volume of the lipid membrane vesicle B containing means for the determination of desired effect of the candidate compounds. The system for high throughput screening of candidate compounds, comprises a first type of lipid membrane vesicle A containing a determined quantity of functional channel protein complex in the lipid membrane and containing a reaction partner I in a medium in internal volume of the lipid membrane vesicle A, a second type of lipid membrane vesicle B containing functional channel protein complex in the lipid membrane and containing a reaction partner II in internal volume of the lipid membrane vesicle B containing means for the determination of desired effect of the candidate compounds to be tested, a device for the determination of desired effect of the candidate compounds to be tested, a device for the determination of the reaction between the reaction partner I from the lipid membrane vesicle A and the reaction partner II from the lipid membrane vesicle B, where the lipid membrane vesicle A and lipid membrane vesicle B are formed through the channel protein complex to be tested in the lipid membrane vesicle B, or the lipid membrane vesicle A and the lipid membrane vesicle B are formed through a protein complex of a channel through which the reaction partner arrives in the interior volume of the respective another lipid membrane vesicle, and means for supplying the candidate compounds to be tested in order to modulate the formation of the channel through the channel protein complex present in the lipid membrane. The reaction partner I guides with the reaction partner II to a demonstrable reaction. The lipid membrane vesicle A and the lipid membrane vesicle B. The second type of lipid membrane vesicle B is a humane cell. The functional channel protein complex is formed from connexins. The channel protein complexes are identical in lipid membrane vesicle A and B. The lipid membrane vesicle A and/or B exists in the form of nanovesicle in a size of 40-80 nM. The means for the determination of desired effect or the reaction partner exists in the interior volume of the lipid membrane vesicle except the buffer or the medium only the candidate compounds to be tested. Independent claims are included for: (1) a method for high throughput screening of candidate compounds; and (2) a lipid membrane vesicle having lipids for forming lipid membrane or channel protein complex such as connexons, innexons or pannexons.