Patent EA-002743-B1 (EA)
Title: Moraxella catarhalis outer membrane protein-106 polypeptide, dna encoding thereof, and use thereof
Inventor: TUCKER KENNETH
IPC: A61K39/00
Filed: 1997-04-28
Abstract: 1. An isolated OMP106 polypeptide, which is an outer membrane polypeptide of Moraxella catarrhalis, and has a molecular weight of about 180 kD to about 230 kD as determined in SDS polyacrylamide gel electrophoresis using rabbit skeletal muscle myosin and E. coli yff-galactosidase as the 200 kD and 116.25 kD molecular weight standards, respectively, and which comprises the sequence of SEQ ID NO: 1 or SEQ ID NO: 1 and SEQ ID N0:2. 2. The isolated OMP106 polypeptide of claim 1, which comprises the sequences of SEQ ID NO:l and SEQ ID NO:2. 3. The isolated OMP106 polypeptide of claim 1 or 2, which has a molecular weight of about 190 kD. 4. The isolated OMP106 polypeptide of any one of claims 1 to 3, which is an outer membrane polypeptide ofMoraxella catarrhalis strain selected from the group consisting of ATCC 25238, ATCC 25240, ATCC 43617, ATCC 43618, ATCC 43627. ATCC 43628 and ATCC 49143. 5. The isolated OMP106 polypeptide of any one of claims 1 to 4, which Moraxella catarrhalis strain is ATCC 49143. 6. The isolated OMP106 polypeptide of any one of claims 1 to 5, wherein the Moraxella catarrhalis is a hemagglutinating cultivar. 7. The isolated OMP106 polypeptide of any one of claim 1 to 6, which reacts with silver stain. 8. An isolated antibody that specifically binds the OMP 106 polypeptide of any one of claims 1 to 7 or a fragment thereof. 9. The isolated antibody of claim 8, which is a cytotoxic antibody that mediates complement killing ofMoraxella catarrhalis. 10. A peptide fragment of the OMP 106 polypeptide of any one of claims 1 to 7, which specifically binds to an antibody of claim 8 and comprises the amino acid sequence of SEQ ID NO:5. 11. A vaccine comprising the OMP 106 polypeptide of any of claims 1 to7. 12. A vaccine comprising the peptide fragment of claim 10. 13. An antigenic composition comprising the OMP 106 polypeptide of any of claims 1 to 7. 14. An antigenic composition comprising the peptide fragment of claim 10. 15. An isolated DNA encoding the isolated OMP106 polypeptide of any one of claims 1 to 7 comprising the sequence of SEQ ID NO:l or the sequences of SEQ ID NO:l and of SEQ ID NO:2, derived on the base of the principle of a genetic code. 16. An isolated DNA encoding the OMP106 polypeptide of any one of claims 1 to 7 comprising sequence of the SEQ ID NO:l, derived on the base of the principle of a genetic code. 17. An isolated DNA encoding the peptide fragment of claim 10, comprising comprising the sequence of SEQ ID NO:4, and also variants obtained on the base of degeneracy of a genetic code. 18. A method of producing an immune response in an animal comprising immunizing the animal with an effective amount of the separated OMP106 polypeptide according to any one of claims 1 to 7. 19. A method of producing an immune response in an animal comprising immunizing the animal with an effective amount of a peplide fragment of claim 10. 20. A method of producing an immune response in an animal of claim 18 for treating an infection with M. catarrhalis comprising immunizing the animal in need thereof with an effective amount of a separated polypeptide of any of claims 1 to 7. 21. A method of producing an immune response in an animal of claim 18 for preventing an infection with M. catarrhalis comprising immunizing the animal in need thereof with an effective amount of a vaccine of claim 11. 22. A method of producing an immune response in an animal of claim 19 for treating an infection with M. catarrhalis comprising immunizing the animal in need thereof with an effective amount of a pertide fragment of claim 10. 23. A method of producing an immune response in an animal of claim 19 for preventing an infection with M. catarrhalis comprising immunizing the animal in need thereof with an effective amount of an antigenic composition of claim 14. 24. The use of a separated polypeptide according to any one of claims 1 to 7 in the preparation of a medicament for the treatment or prevention of infection with M. catarrhalis. 25. The use of an isolated antibody according to any one of claims 8 to 9 in the preparation of a medicament for the treatment or prevention of infection with M. catarrhalis. 26. The use of a peptide fragment according to claim 10 in the preparation of a medicament for the treatment or prevention of infection with M. catarrhalis. 27. The use of a DNA according of claim 17 in the preparation of a medicament for the treatment or prevention of infection with M, catarrhalis.