Patent SG-11201407866X-A (SG)
Title: Method for expression of heterologous proteins using a recombinant negative-strand rna virus vector
Inventor: WIEGAND MARIAN
IPC: C12N15/86
Filed: 2013-05-24
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 5 December 2013 (05.12.2013) WIPOIPCT (10) International Publication Number WO 2013/178344 A1 (51) International Patent Classification: C12N15/86 (2006.01) C12N 5/071 (2010.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/EP2013/001547 24 May 2013 (24.05.2013) English English (30) Priority Data: 12004148.8 61/652,913 30 May 2012 (30.05.2012) EP 30 May 2012 (30.05.2012) US (71) Applicant: AMVAC AG [CH/CH]; Metallstr. 4, CH-6300 Zug (CH). (72) Inventor: WIEGAND, Marian; Milchstrasse 1, 81667 Muenchen (DE). (74) Agent: RICKER, Mathias; Wallinger Ricker Schlotter Tostmann, Zweibriickenstr. 5-7, 80331 Muenchen (DE). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) (54) Title: METHOD FOR EXPRESSION OF HETEROLOGOUS PROTEINS USING A RECOMBINANT NEGAT- IVE-STRAND RNA VIRUS VECTOR •t •t m oo i> i-H cn i-H o CJ o & u (0 PS 03 •Q 0.8 0.6H 0.4 0.2 u .> ro g 0.015- g- 0.010- ifl 0.005- v D. 0.000- x 07 r* i & £ Nanog BBS Pi •m fsssa ^ day after transduction Fig. 3 m 1 m (57) Abstract: The present invention provides a method of expressing at least one heterologous nucleic acid sequence in a cell, the method comprising introducing at least one het­ erologous nucleic acid sequence into cell a by infecting said cell with a recombinant negative-strand RNA virus vector comprising said at least one heterologous nucleic acid se­ quence, wherein the recombinant negative- strand RNA virus vector includes a viral genome coding for a mutated P pro - tein, which leads to a loss of the viral genome replication ability without a loss of the viral transcription ability, and wherein said at least one heterologous nucleic acid sequence encodes cellular reprogramming a or programming factor or a therapeutic protein. In addition, the present invention provides a cell or a population of cells prepared in vitro by said method as well as a pharmaceutical composition com­ prising said cell or population of cells.