Patent SG-11201804144U-A (SG)
Title: High-throughput imaging-based methods for predicting cell-type-specific toxicity of xenobiotics with diverse chemical structures
Inventor: LOO LIT-HSIN
IPC: G06T7/00
Filed: 2016-11-09
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) CORRECTED VERSION (19) World Intellectual Property Organization International Bureau .9 . si ww..0.7,..d ei (43) International Publication Date issor 1 June 2017 (01.06.2017) WIPO I PCT ID Hit (10) WO International 2017/091147 111111111111111111111111111111111111111111111111111111111111111t111111111111111 Publication A8 Number (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every G01N 33/50 (2006.01) G06T 7/00 (2017.01) kind of national protection available): AE, AG, AL, AM, G06F 19/00 (2011.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (21) International Application Number: DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, PCT/SG2016/050554 HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, (22) International Filing Date: KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 9 November 2016 (09.11.2016) MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (25) Filing Language: English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, (26) Publication Language: English TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 10201509598X 20 November 2015 (20.11.2015) SG (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, Connexis, Singapore 138632 (SG). TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, (72) Inventors: LOO, Lit-Hsin; c/o Bioinformatics Institute, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, 30 Biopolis St, Matrix, #07-01, Singapore 138671 (SG). TR), BJ, SM, OAPI (BF, CF, CG, CI, CM, GA, GN, GQ, LEE, Jia Ying; c/o Bioinfoiinatics Institute, 30 Biopolis GW, KM, ML, MR, NE, SN, TD, TG). St, Matrix, #07-01, Singapore 138671 (SG). SU, Ran; c/o Bioinfoiinatics Institute, 30 Biopolis St, Matrix, #07-01, Published: Singapore 138671 (SG). ZINK, Daniele; c/o Institute of with international search report (Art 21(3)) Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, #04-01, Singapore 138669 (SG). XIONG, (48) Date of publication of this corrected version: Sijing; c/o Institute of Bioengineering and Nanotechno- 29 June 2017 logy, 31 Biopolis Way, The Nanos, #04-01, Singapore (15) Information about Correction: 138669 (SG). see Notice of 29 June 2017 Agent: MATTEUCCI, Gianfranco; Marks & Clerk (74) Singapore LLP, Tanjong Pagar, P.O. Box 636, Singapore 910816 (SG). GC .4 t IN it 1-1 (54) Title: HIGH-THROUGHPUT IMAGING-BASED METHODS FOR PREDICTING CELL-TYPE-SPECIFIC TOXICITY OF Il cp \ XENOBIOTICS WITH DIVERSE CHEMICAL STRUCTURES 0 ---- (57) : The present invention provides methods for the prediction of in vivo cell-specific toxicity of a compound that com - I N biases high-throughput imaging of cultured cells, quantitative phenotypic profiling, and machine learning methods. More particularly, Il c:::, the invention provides a method for the prediction of in vivo renal proximal tubular-, bronchial-epithelial-, and alveolar-cell-specific N toxicities of a soluble or particulate compound that comprises contacting cultured human kidney and pulmonary cells with the com- 0 pound at a range of concentrations, then labelling the cells with DNA, yH2AX and actin markers and obtaining textural features, spatial correlation features, ratios of the markers, intensity features, cell count and morphology, estimating dose response curves and performing automatic classification of the compound using a random-forest algorithm.