Patent DE-102011120550-A1 (DE)
Title: Detecting adenovirus target nucleic acid, by contacting sample with amplification oligomer to obtain amplicons, providing conditions sufficient to produce amplicons of adenovirus target nucleic acid, and detecting amplicons
Inventor: ZIEGLER EMILY L
IPC: C12Q1/68
Filed: 2011-12-05
Abstract: Method for specifically detecting adenovirus target nucleic acid in a sample, involves (a) contacting a sample containing at least one adenovirus nucleic acid with at least two amplification oligomer to obtain amplicons, (b) providing conditions sufficient to produce amplicon of adenovirus target nucleic acid present in the sample using amplification oligomer, and detecting the amplicons and determining whether an adenovirus target nucleic acid is present in the sample. Method for specifically detecting adenovirus target nucleic acid in a sample, involves (a) contacting a sample containing at least one adenovirus nucleic acid with at least two amplification oligomer to obtain amplicons, where each of at least two amplification oligomer has length of 10-50 nucleotides, and the amplification oligomer is capable to specifically bind with target nucleic acid of adenovirus nucleic acid at positions 1-99 and 83-175 of a 2775 base pair sequence (SEQ ID NO: 47) of accession number AB330090.1, and (b) providing conditions sufficient to produce amplicon of the adenovirus target nucleic acid present in the sample using amplification oligomer, and detecting the amplicons and determining whether an adenovirus target nucleic acid is present in the sample. Independent claims are included for the following: (1) composition comprising combination of amplification oligomers chosen from SEQ ID NOs: 25, 26, 27 and 28, SEQ ID NOs: 26, 27 and 28, SEQ ID NOs: 25, 26 and 28, SEQ ID NOs: 25, 26 and 27, SEQ ID NOs: 25, 27 and 28, SEQ ID NOs: 25 and 27, SEQ ID NOs: 25 and 28, SEQ ID NOs: 26 and 27, and SEQ ID NOs: 26 and 28; (2) kit comprising the above-mentioned composition; and (3) isolated DNA sequence comprising base pair sequence of SEQ ID NOs: 1-46, or its corresponding isolated RNA sequence. caggacgcctcgga/ggtat/cctg/cag (SEQ ID NO: 1), ggagccavgtggga/gtt (SEQ ID NO: 2), aat/ccccacg/c/tgtggctcc (SEQ ID NO: 3), ccgggtctggtgcagtttgcccgc (SEQ ID NO: 4), cacatcgccggacagga (SEQ ID NO: 5), catactgaagtaggtgtctgt (SEQ ID NO: 6), acagacacctacttcagtatg (SEQ ID NO: 7), cggtggtcacatcgtgg (SEQ ID NO: 8), ccacgatgtgaccaccg (SEQ ID NO: 9), agtacctcagtccgggtctggtg (SEQ ID NO: 10), atggctaccccttcgatg (SEQ ID NO: 11), acccca/ctcgatgatgcc (SEQ ID NO: 12), gcgggcgaattgcacca (SEQ ID NO: 13), tggtgcaattcgcccgc (SEQ ID NO: 14), gcgggcaaat/ctgcacca (SEQ ID NO: 15), tggtgcaa/gtttgcccgc (SEQ ID NO: 16), gactcaggtactccgaagcatcct (SEQ ID NO: 17), aggatgcttcggagtacctgagtc (SEQ ID NO: 18), ctcaggtactccgaggcgtcct (SEQ ID NO: 19), aggacgcctcggagtacctgag (SEQ ID NO: 20), ctcaggtactccgaagcatcct (SEQ ID NO: 21), aggatgcttcggagtacctgag (SEQ ID NO: 22), caggtactccgaggcgtcct (SEQ ID NO: 23), aggacgcctcggagtacctg (SEQ ID NO: 24), accccatcgatgatgcc (SEQ ID NO: 25), accccctcgatgatgcc (SEQ ID NO: 26), gcgggcaaactgcacca (SEQ ID NO: 27), gcgggcaaattgcacca (SEQ ID NO: 28), ctcaggtattccgaggcatcct (SEQ ID NO: 29), aggatgcctcggaatacctgag (SEQ ID NO: 30), accccatcgatgctgcc (SEQ ID NO: 31), accccatcgatgatgcc (SEQ ID NO: 32), tgggcgtacatgcacatc (SEQ ID NO: 33), gtggtcttacatgcacatc (SEQ ID NO: 34), gtgggcatacatgcacatc (SEQ ID NO: 35), aggatgcttcggagtacctgag (SEQ ID NO: 36), aggacgcctcggagtacctg (SEQ ID NO: 37), aa/gtggg/tcdtacatgcacatc (SEQ ID NO: 38), caggacgcctcggagtacct (SEQ ID NO: 39), aggatgcttcggagtacctgag (SEQ ID NO: 40), cacgatgtgaccacaga (SEQ ID NO: 41), cat/cgatgtgaccacaga (SEQ ID NO: 42), cacgat/cgtgaccacaga (SEQ ID NO: 43), cacgatgtgaccacg/cga (SEQ ID NO: 44), cacgatgtgaccacg/a/cga (SEQ ID NO: 45) and cat/cgat/cgtgaccacg/a/cga (SEQ ID NO: 46).