Patent SG-11201900057W-A (SG)
Title: Biomatrix scaffolds for use in diagnosing and modeling cancer
Inventor: WANG ANDREW ZHUANG
IPC: G01N33/574
Filed: 2017-07-11
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 111111101111 0 1110101011111 011101 0 0111111 011101111001111101111111011110111111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\" WO 2018/013542 Al 18 January 2018 (18.01.2018) W I P0 I P C T (51) International Patent Classification: (74) Agent: VILLACORTA, Gilberto M. et al.; FOLEY & A61K 35/12 (2015.01) G01N 33/00 (2006.01) LARDNER LLP, 3000 K Street N.W., Suite 600, Washing- C12Q 1/00 (2006.01) G01N 33/574 (2006.01) ton, District of Columbia 20007-5109 (US). (21) International Application Number: (81) Designated States (unless otherwise indicated, for every PCT/US2017/041500 kind of national protection available): AE, AG, AL, AM, (22) International Filing Date: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, 11 July 2017 (11.07.2017) CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (25) Filing Language: English HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, (26) Publication Language: English KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (30) Priority Data: OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, 62/361,306 12 July 2016 (12.07.2016) US SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, 62/460,056 16 February 2017 (16.02.2017) US TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (71) Applicant: THE UNIVERSITY OF NORTH CAROLI- (84) Designated States (unless otherwise indicated, for every NA AT CHAPEL HILL [US/US]; Office of Commer- kind of regional protection available): ARIPO (BW, GH, cialization and Economic Development, 100 Europa Drive, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, Suite 430, Chapel Hill, North Carolina 27517 (US). UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, (72) Inventors: WANG, Andrew Zhuang; 136 Turner Ridge EE, ES, FL FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, Road, Durham, North Carolina 27713 (US). TIAN, Xi; NO, PL, PT, RO, RS, SE, SI, SK, SM, — MC, MK, MT, NL, CF, CG, CI, CM, GA, GN, GQ, GW, = 2236 Marsico Hall, 125 Mason Farm Road, Chapel Hill, TR), OAPI (BF, BJ, SN, TD, TG). North Carolina 27599 (US). REID, Lola M.; 3621 Sweeten KM, ML, MR, NE, Creek Road, Chapel Hill, North Carolina 27514 (US). = = (54) Title: BIOMATRIX SCAFFOLDS FOR USE IN DIAGNOSING AND MODELING CANCER = = Figure 1 a ' maratherapeutic.•sc - ng obtforintor tissues = = = ecellularialion = = = = — Metastases dish on a F . = cells 11 .4 13:0r113I:EA il, I disk ei ii- in (57) : Described herein are in vitro cancer metastasis models that recapitulate biological facets of organ specificity as indicated en by the behavior of human colorectal cancer cells when on substrata of liver and lung biomatrix scaffolds, organ-specific matrix extracts C generated by novel decellularization strategies. Tumor cells spontaneously formed colonies that mimicked in vivo metastatic lesions ---, in terms of histology, biology and genetic and other phenotypic traits. Not to be bound by theory, it is postulated that responses of * 1-1 1:) tumors to radiation or chemotherapeutic agents proved dependent on the matrix substratum used; thus, thus the scaffolds are believed cz to provide a powerful tool for cancer research and diagnosis. ei O [Continued on next page] WO 2018/013542 Al MIDEDIMOHNOBEIENIRMIOMINIEHIMEHOE# Declarations under Rule 4.17: — as to applicant's entitlement to apply patent (Rule 4.17(11)) for and be granted a Published: — with international search report (Art. 21(3))