Patent SG-11201809650T-A (SG)
Title: Culture media for multipotent stem cells
Inventor: PARK HYEONGGEUN
IPC: C12N5/0775
Filed: 2017-05-01
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property C.--.` Organization 03 11111111111111111111111111111111111111111111111111111111111111111111111111111 International Bureau (10) International Publication Number (43) International Publication Date ......•' WO 2017/190137 Al 02 November 2017 (02.11.2017) W I PO I PCT (51) (21) (22) (25) Filing Language: (26) (30) (71) (72) (74) International Patent Classification: HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, C12N 5/0735 (2010.01) C12N 5/0775 (2010.01) KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, International Application Number: MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PCT/US2017/030410 PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, International Filing Date: TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. 01 May 2017 (01.05.2017) (84) Designated States (unless otherwise indicated, for every English kind of regional protection available): ARIPO (BW, GH, Publication Language: English GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, Priority Data: TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, 15/142,135 29 April 2016 (29.04.2016) US EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, 15/460,111 15 March 2017 (15.03.2017) US MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, Applicant: HOPE BIOSCIENCES, LLC [US/US]; KM, ML, MR, NE, SN, TD, TG). 16700 Creekbend Drive, Sugar Land, Texas 77478 (US). Inventor: PARK, Hyeonggeun; 16700 Creekbend Drive, Published: Sugar Land, Texas 77478 (US). — with international search report (Art. 21(3)) Agent: RAO, Dileep P. et al.; Rao Deboer Osterrieder, PLLC, 2550 Gray Falls Drive, Suite 200, Houston, Texas 77077 (US). Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, _— = (81) = _ _ _ = = Title: CULTURE MEDIA FOR MULTIPOTENT STEM CELLS (57) : A growth medium for culturing mesenchymal stem cells. The medium comprises a serum, a fibroblast growth factor, and either an L-cys- teine, a glutathione, or an N-acetyl cysteine. Optionally, the medium can comprise various quantities of an epidermal growth factor, a hydrocortisone, a calcium chloride, an insulin, a pituitary extract, a selenium, a stromal-de- factor, a sodium pyruvate, a transferrin, and serum-free medium. (54) 20 17 / 190 137 Al 1 11 111 1111111111 111111 11111 1 111111111 11 11 1 11 111 11111 1111 REAGENTS COMPONENT RANGES A SAMPLE EMBODIMENT SERUM FREE MEDIUM BALANCE T0100% VOL BALANCE TO 100% VOL SERUM 0.1 - 50% VOL - 10% VOL FIBROBLAST GROWTH FACTOR 1 pg/mL - 100 ng/mL 1 - 10 ng/mL rived EPIDERMAL GROWTH FACTOR 1 pg/mL -100 ng/mL 1 -10 ng/mL HYDROCORTISONE 1 pg/mL - 100 lig/mL 10 -100 ng/mL CALCIUM CHLORIDE 1 nM - 100 mM 0.01 - 0.1 mM INSULIN 1 ng/mL - 100 mg/mL 0.5 - 5 mg/mL PITUITARY EXTRACT 1 pg/mL - 100 mg/mL 10 - 100 µg/mL L-CYSTEINE DR GLUTATHIONE 1 nM - 100 mM 0.5 - 5 RIM SELENIUM 1 pg/mL - 100 mg/mL 0.1 - 1 pg/mL STROMAL CEL-DERIVED FACTOR 1 pg/mL - 100 ng/mL 1 - 10 ng/mL SODIUM PYRUVATE 1 ng/mL - 100 mg/mL 2 - 20 mg/mL TRANSFERRIN 1 ng/mL - 100 mg/mL 0.1 -1 mg/mL FIG. I C