Patent EP-2287336-A1 (EP)
Inventor: EINSTEIN M RICH
IPC: C12Q1/68
Filed: 2009-07-31
Abstract: Detecting the presence of Alzheimer's disease in a mammal, involves contacting, under conditions allowing hybridization between complementary sequences, nucleic acids from a blood sample from the mammal and a set of probes comprising (C1) 10 specific probes or their complementary strand, to obtain an expression profile, where the expression profile is characteristic of the presence of Alzheimer's disease in the mammal. Detecting the presence of Alzheimer's disease in a mammal, involves contacting, under conditions allowing hybridization between complementary sequences, nucleic acids from a blood sample from the mammal and a set of probes, where the set of probes comprises (C1): at least one probe comprising all or part of (SEQ ID NO: 34) (SEQ ID NO: not defined) or its complementary strand; at least one probe comprising all or part of (SEQ ID NO: 230) (SEQ ID NO: not defined) or its complementary strand; at least one probe comprising all or part of (SEQ ID NO: 341) (SEQ ID NO: not defined) or its complementary strand; at least one probe comprising all or part of (SEQ ID NO: 454) (SEQ ID NO: not defined) or its complementary strand; at least one probe comprising all or part of (SEQ ID NO: 664) (SEQ ID NO: not defined) or its complementary strand; at least one probe comprising all or part of (SEQ ID NO: 811) (SEQ ID NO: not defined) or its complementary strand; at least one probe comprising all or part of (SEQ ID NO: 951) (SEQ ID NO: not defined) or its complementary strand; at least one probe comprising all or part of (SEQ ID NO: 1127) (SEQ ID NO: not defined)or its complementary strand; at least one probe comprising all or part of (SEQ ID NO: 1136) (SEQ ID NO: not defined) or its complementary strand; and at least one probe comprising all or part of (SEQ ID NO: 1752) (SEQ ID NO: not defined) or its complementary strand, to obtain an expression profile, where the expression profile is characteristic of the presence of Alzheimer's disease in the mammal and the above part comprising at least 15 consecutive nucleotides of the reference sequence. Independent claims are included for the following: (1) a product (p1) comprising a support on which a set of probes is immobilized, the set comprising (C1) which contains at least 15 consecutive nucleotides of the reference sequence; (2) a product (p2) comprising a support on which are immobilized at least two distinct nucleic acid probes, where each probe comprises a sequence complementary to and/or specific of a target nucleic acid selected from (SEQ ID NOs: 1-1754) (SEQ ID NOs: not defined) or its complementary strand; (3) a kit comprising a compartment or container comprising at least 5, 10, 20, 30, 40, 50, 60 or more distinct nucleic acids selected from the nucleic acids of (C1); (4) a kit comprising the product (p1) or (p2) and reagents for a hybridization reaction; (5) detecting the presence of Alzheimer's disease in a mammal, involving contacting, under conditions allowing an amplification reaction, nucleic acids from a blood sample from the mammal and a set of primers, where the set of primers comprises primers containing all or part of each of the following target nucleic acid sequences, or its complementary strand: (SEQ ID NOs: 34, 230, 341, 454, 664, 811, 951, 1127, 1136 and 1752), to obtain an amplification profile or product, the amplification profile or product being characteristic of the presence of Alzheimer's disease in the mammal.