Patent SG-11201901410S-A (SG)
Title: Human otic progenitor identification and isolation
Inventor: RIVOLTA CARLOS MARCELO NICOLAS
IPC: G01N33/569
Filed: 2017-09-14
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 111111111111111111111111111 1111111111111111 H 11111110111010111111101111 0111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\"\" WO 2018/051092 Al 22 March 2018 (22.03.2018) WIP0 I PCT (51) International Patent Classification: G01N 33/569 (2006.01) C12N 5/0793 (2010.01) Declarations under Rule 4.17: C07K 16/28 (2006.01) C12N 5/0797 (2010.01) — of inventorship (Rule 4.17(iv)) C07K 16/30 (2006.01) C12N 5/074 (2010.01) Published: (21) International Application Number: — with international search report (Art. 21(3)) PCT/GB2017/052707 (22) International Filing Date: 14 September 2017 (14.09.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 1615714.1 15 September 2016 (15.09.2016) GB (71) Applicant: THE UNIVERSITY OF SHEFFIELD [GB/GB]; Firth Court, Western Bank, Sheffield S10 2TN (GB). (72) Inventors: RIVOLTA, Carlos Marcelo Nicolas; c/o The University of Sheffield Biomedical Sciences, Firth Court, Western Bank, Sheffield South Yorkshire S10 2TN (GB). BODDY, Sarah Louise; c/o The University of Sheffield, Firth Court, Western Bank, Sheffield South Yorkshire S10 2TN (GB). Agent: HGF LTD; Document Handling (HGF) York, 1 (74) City Walk, Leeds Yorkshire LS11 9DX (GB). Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (81) — Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). = (84) 1-1 ei cT 1-1 © (54) Title: HUMAN OTIC PROGENITOR IDENTIFICATION AND ISOLATION ItI n (57) : The present invention relates generally to the identification and isolation of human otic progenitor cells. More specifi- c= ----. cally, the present invention relates to a method of using cell markers to identify and isolate human otic progenitor cells from a mixed cie , 1 population of cells, methods of enrichment and production of human otic progenitor cells, and associated kits for use in identification © and/or isolation of human otic progenitor cells, wherein the cell markers are selected from SSEA1 (CD15), disialoganglioside GD3, N TRA-2-49 (liver/bone/ kidney alkaline phosphatase), SSEA4, ganglioside GD2 and CD141. C