Patent DE-102009039868-A1 (DE)
Title: Arrangement of explosion devices for carrying out a process for monitoring the effect of a gaseous medium on a biological test system under use of an extra cellular metabolizing system, comprises a permeable carrier
Inventor: KNEBEL JAN
IPC: C12M3/04
Filed: 2009-09-03
Abstract: The arrangement of explosion devices (10) for carrying out a process for monitoring the effect of a gaseous medium on a biological test system (28) under use of an extracellular metabolizing system, comprises a permeable carrier (30) that is arranged on the biological test system over which a gaseous medium is introduced over the surface of the test system for forming an explosion atmosphere above the test system, and a supply system (40) that consists of maintenance medium with extracellular metabolizing system. The arrangement of explosion devices (10) for carrying out a process for monitoring the effect of a gaseous medium on a biological test system (28) under use of an extracellular metabolizing system, comprises a permeable carrier (30) that is arranged on the biological test system over which a gaseous medium is introduced over the surface of the test system for forming an explosion atmosphere above the test system, and a supply system (40) that consists of maintenance medium with extracellular metabolizing system. The maintenance medium is directly connected with the metabolizing system with the lower side of the permeable carrier, which is arranged within the explosion device. The carrier separates the maintenance medium with the extracellular metabolizing system physically from the explosion atmosphere. The maintenance medium compressed with the metabolizing system by a given pressure defined only though the permeable carrier. The biological test system is overflowed by the maintenance medium with extracellular metabolizing system. The explosion device exists in the form of a microtiter and/or microwave plate. The explosion devices are arranged in such a way that it has an external dimension corresponding to the microtiter and/or microwave plate. The individual explosion devices are dimensioned so that the microtiter and/or microwave plates are arranged on the base surface. The explosion devices are arranged in such a way that the monitoring process is automatically carried out by a pipetting robot or in such a way that a fluid medium is provided with the metabolizing system. The explosion device has a common carrier plate in which a channel system (24) is arranged for the supply and discharge of the fluid medium such as maintenance medium with extracellular metabolizing system. The channel system connected to the explosion device is loadable and/or arranged with a defined and/or pre-determined pressure. The channel system is arranged in such a way that it is provided dependent of its direction and/or flow in the carrier plate of the arrangement and/or dependent of the maintenance medium in all direction of the arrangement. The metabolizing system is fixed on the limiting surface of the permeable carrier. The arrangement is formed in such a way that the incubation of the medium carrying in an incubator during transporting or storing adjoins with a constant pressure on the permeable carrier. The arrangement has a pump. The explosion device and/or the arrangement is formed in such a way that a microscopic observation of the permeable carrier is carried out through the lower side of the explosion device and/or the arrangement. The explosion device and/or the arrangement is equipped at the lower side with components so that an optical online-observation such as fluorescence is carried out without utilizing microscope under use of laser- and/or light conductive technique and spectrometers. A top piece is provided that covers the explosion device in such away that a gaseous medium is guidable with and/or without solid material and fluid portion such as solid-aerosol or particle aerosol, and/or further gaseous medium by the permeable carrier arranged in the explosion device. The individual explosion device has a sensor by which oxygen, glucose and/or carbon dioxide content is determined by transepithelial electric resistance in the form of an online-measurement. The explosion device consists of autoclavable material. A heating device is provided through which the arrangement is partially or completely heated. On the top piece, a low pressure system is connected through which the negative pressure is distributed on the explosion device. The top piece has a physical arrangement in which the pressure drop adjusts over a flow limitation in the form of a capillary or critical nozzle and the explosion flow over the individual explosion device on a defined and/or determined value. The supply of the explosion and/or test atmosphere is carried out in the tip piece. The sensors are arranged in the top piece by which the online measurement of temperature, pressure or humidity is carried out. The explosion device has a multiwave plate-compatible system such as multiwave plate reader or microscope.