Patent GB-695273-A (GB)
Title: Method for the production of vitamin substances
IPC: C07H23/00
Filed: 1949-07-11
Abstract: In the production of vitamin B12 or other L.L.D. active substances by fermenting a nutrient medium by means of an L.L.D. activity-producing strain of a micro-organism of the subphylum Fungi, the proportion of L.L.D.-active substances relative to the total broth solids is improved by including cobalt in non-toxic concentration (e.g. 0.1-20, preferably about 2, parts per million) in the medium.  Preferred micro-organisms are Schizomycetes such as those of the genera Streptomyces (e.g. S. albidoflavus, S. columbiensis, S. roseochromogenus, S. antibioticus, or especially S. griseus which may simultaneously produce streptomycin or grisein) and Clostridium (e.g. C. tetanomorphum, C. cochlearium, C. flabelliferum or C. butyricum). Also suitable are Torula, Eremothecium ashbyii and Escherichia coli. The nutrient medium may contain a source of assimilable carbon, a source of assimilable nitrogen, and inorganic salts including a soluble cobalt salt (e.g. cobalt nitrate).  The fermentation may be carried out under submerged aerated conditions at about 28 DEG  C. for 2-7 days, and the vitamin B12 may be separated from the broth after fermentation by filtering and treating the filtrate with adsorbent carbon. Examples show the effect of various concentrations of cobalt in the fermentation with S. griseus of aqueous media containing: (1) a suspension of dried yeast; (2) protein meat and bone scrap, sodium chloride and ferrous sulphate; (3) tryptic digest of casein, meat extract, sodium chloride, ferrous sulphate and soybean oil; (4) soybean meal, dextrose, sodium chloride and distillers solubles; (5) brain-heart infusion and agar.  Reference is also made to the use of nutrient media containing maltose, xylose, invert sugar, corn syrup, ammonium salts, amino acids, oats, yeast extract, blood meal, salmon meal, fish meals and fish solubles, and of solid nutrient media such as grain brans. Specification 635,529 is referred to.ALSO:Animal foods are enriched with the solid content of broths in which LLD activity-producing strains of micro-organisms of the sub-phylum fungi (especially Streptomyces grisens) have been cultivated in the presence of cobalt in non-toxic concentration. Specification 635,529, [Group IV(b)], is referred to.ALSO:In the production of vitamin B12 or other LLD active substances by fermenation, LLD activity-producing strains of micro-organisms of the sub-phylum Fungi are cultivated in a nutrient medium containing cobalt in non-toxic concentration (e.g. 0.1-20, preferably about 2, parts per million).  Preferred micro-organisms are Schizomycetes such as those of the genera Streptomyces (e.g. S. albidoflavus, S. colombiensis, S. roseochromogenus, S. antibioticus or especially S. griseus) and Clostridium (e.g. C. tetanomorphum, C. cochlearium, C. flabelliferum or C. butyricum).  Also suitable are Eremothecium ashbyii and Escherichia coli.  The nutrient medium may contain a source of assimilable carbon, a source of assimilable nitrogen, and inorganic salts including a soluble cobalt salt (e.g. cobalt nitrate).  The fermentation may be carried out under submerged aerated conditions at about 28 DEG  C. for 2-7 days.  Examples describe the cultivation of S. griseus in the presence of various concentrations of cobalt in aqueous media containing: (1) a suspension of dried yeast; (2) protein meat and bone scrap, sodium chloride, ferrous sulphate and soy bean oil; (4) soy bean meal, dextrose, sodium chloride and distillers solubles; (5) brain-heart infusion and agar.  Reference is also made to the use of nutrient media containing maltose, xylose, invert sugar, corn syrup, ammonium salts, amino acids, oats, yeast extracts, blood meal, salmon meal, fish meals and fish solubles, and of solid nutrient media such as grain bran.  Specification 635,529 is referred to.