a total of 20 male type 2 diabetic patients treated with oral glucose  lowering medication ( n = 19 ) or diet only ( n = 1 ) participated in the current study .
exclusion criteria were self - reported renal failure and liver disease ( hepatitis and cirrhosis ) , morbid obesity ( bmi > 40 kg / m ) , uncontrolled hypertension ( > 160 mmhg systolic or > 100 mmhg diastolic or both ) , and a history of severe cardiovascular problems ( myocardial infarction in the past year or stroke ) .
all subjects were informed about the nature and the risks of the experimental procedures before their written informed consent was obtained .
after an overnight fast , subjects arrived at the laboratory at 0800 h by car or public transportation .
a fasting blood sample was obtained , after which a standard 75-g oral glucose tolerance test was performed to confirm type 2 diabetes according to the american diabetes association criteria ( 24 ) .
after blood sampling , all subjects performed an incremental exercise test on a cycle ergometer ( lode excalibur ) to determine their maximal workload capacity .
subjects participated in a randomized crossover study consisting of three intervention periods separated by at least 1 week .
each intervention period consisted of 3 days , during which blood glucose homeostasis was assessed under standardized dietary conditions ( supplementary fig .
, subjects reduced their sedentary behavior either by three 15-min bouts of adl ( postmeal strolling ; 3 mets ) or by a single 45-min bout of moderate - intensity cycling exercise ( 6 mets ) . on day 1 of each intervention period
, participants arrived at the laboratory during the afternoon and received a short period of training in the use of the capillary blood sampling method ( glucocard x meter ; arkray , kyoto , japan ) .
subsequently , a continuous glucose - monitoring device ( glucoday s ; a. menarini diagnostics , florence , italy ) was attached as described previously ( 7 ) and participants returned home . on day 2 , participants arrived at the laboratory by car at 0730 h after an overnight fast .
an intravenous catheter was inserted into an antecubital vein for blood sampling purposes , and participants were equipped with a heart rate monitor ( polar rs300 ) .
participants received breakfast , lunch , and dinner at 0830 , 1230 , and 1700 h , respectively .
blood samples were collected in prechilled edta - coated tubes 5 min before each meal , and 90 and 150 min after each meal ( total of 9 samples ) .
after the last blood sample at 1930 h subjects went home . on day 3 , subjects reported back to the laboratory during the morning for removal of the continuous glucose - monitoring device .
the three intervention periods were identical with the exception of the three 15-min bouts of adl or the single 45-min bout of endurance - type exercise , which were performed on the second day of the experimental period ( supplementary fig .
participants were restricted to a sedentary laboratory environment and spent the day seated in a chair or couch while reading , talking , viewing television , or working on a laptop computer . during the adl condition , sedentary behavior of the patients
was interrupted by three 15-min bouts of slow - paced strolling ( 3 met ) performed after each main meal ( 0915 , 1315 , and 1745 h ) .
the bouts of strolling were chosen to reflect light adl , such as walking the dog , household tasks , light gardening work , and so on . during each bout ,
the bouts were supervised by a physical therapist . during the exercise condition , participants completed a single 45-min bout of moderate - intensity endurance - type exercise after breakfast ( 0915 to 1000 h ) and were sedentary throughout the remainder of the day . the moderate - intensity exercise bout consisted of continuous cycling performed on a cycle ergometer at 50% of individual maximal workload capacity of the patients , translating to an average absolute workload of 85  4 w ( 6 met ) .
the met values for both the adl and moderate - intensity exercise intervention were estimated based on the compendium of physical activities ( 25 ) .
glucose - lowering medications used by patients are listed in table 1 . except for the screening , intake of all medication
was continued throughout the entire study , including the days that subjects visited the laboratory for testing .
all subjects were asked to maintain their normal physical activity patterns throughout the study but to refrain from exhaustive physical labor and exercise training for 2 days before each experimental period . on day 1 of each intervention period ,
subjects were provided with a standardized dinner and evening snack . on day 2 of each intervention period , the actual test day , participants were served a standardized breakfast ( 0830 h ) , lunch ( 1230 h ) , and dinner ( 1700 h ) in the laboratory , and they consumed a standardized evening snack at home ( 2030 h ) . on day 3 of each intervention period , participants remained fasted until 0830 h ( end of data collection ) , after which they resumed their regular diets .
participant characteristics breakfast and lunch were identical and both meals consisted of bread , margarine , salami slices , cheese , jam , and semi - skim ( 1.5% fat ) milk .
the quantity of the diet ( energy content ) was based on individual daily energy requirements of the patients as calculated with the harris and benedict equation multiplied by a physical activity level value of 1.4 .
the resulting diet provided 9.8  0.1 mj / day , consisting of 50% of energy from carbohydrate , 15% from protein , and 35% from fat .
venous blood samples were collected in prechilled edta - coated tubes and centrifuged at 1,000 g and 4c for 10 min .
aliquots of plasma were immediately frozen in liquid nitrogen and stored at 80c until analyses .
plasma insulin concentrations were determined by a radioimmunoassay for human insulin ( hi-14k ; millipore , ma ) .
whole blood hba1c content was determined in 3 ml venous blood samples by high - performance liquid chromatography ( bio - rad diamat , munich , germany ) .
the data obtained by the continuous glucose monitor were downloaded to a personal computer with glucoday software ( version 3.2.2 ) .
values reported by the continuous glucose - monitoring device were converted into glucose values using the self - monitored capillary blood glucose values , which were obtained at least before each main meal and before night time .
the 24-h glycemic control was derived from the glycemic profiles obtained between 8:30 and 8:30 h on days 2 and 3 of each experimental period and was defined as the average 24-h blood glucose concentration and the prevalence of hyperglycemia over the course of 24 h. based on the american diabetes association / european association for the study of diabetes guidelines for glycemic control ( 26,27 ) , the prevalence of hyperglycemia was defined as total time during which glucose concentrations exceeded 10 mmol / l .
the continuously monitored glycemic profiles obtained on day 2 of each experimental period also were used to assess postprandial glycemic control .
postprandial glycemic control was defined as the incremental area under the curve ( iauc ) above fasting glucose assessed over the 3.5-h postprandial periods after each main meal ( 3.5-h glucose iauc ) , and as the cumulative glucose iauc of all main meals ( 10.5-h glucose iauc ) .
plasma insulin concentrations obtained on day 2 of each experimental period ( from 0830 to 1930 h ) were used to calculate the iauc above fasting plasma insulin by the trapezoidal rule ( 11-h insulin iauc ) .
differences between nontime - dependent variables ( prevalence of hyperglycemia , 24-h glucose concentration , iauc ) were assessed by one - way repeated - measures anova , with treatment as the within - subject factor . in case of a significant main effect ,
a two - way repeated - measures anova with time and treatment as within - subject factors was used to compare differences between treatments over time ( plasma insulin concentrations ) . in case of interaction between significant time and treatment ,
pairwise comparisons with bonferroni correction were applied for separate time points to locate differences between treatments .
a total of 20 male type 2 diabetic patients treated with oral glucose  lowering medication ( n = 19 ) or diet only ( n = 1 ) participated in the current study .
exclusion criteria were self - reported renal failure and liver disease ( hepatitis and cirrhosis ) , morbid obesity ( bmi > 40 kg / m ) , uncontrolled hypertension ( > 160 mmhg systolic or > 100 mmhg diastolic or both ) , and a history of severe cardiovascular problems ( myocardial infarction in the past year or stroke ) .
all subjects were informed about the nature and the risks of the experimental procedures before their written informed consent was obtained .
all patients underwent an oral glucose tolerance test . blood glucose  lowering medication was withheld 2 days before the oral glucose tolerance test . after an overnight fast , subjects arrived at the laboratory at 0800 h by car or public transportation .
a fasting blood sample was obtained , after which a standard 75-g oral glucose tolerance test was performed to confirm type 2 diabetes according to the american diabetes association criteria ( 24 ) .
after blood sampling , all subjects performed an incremental exercise test on a cycle ergometer ( lode excalibur ) to determine their maximal workload capacity .
subjects participated in a randomized crossover study consisting of three intervention periods separated by at least 1 week .
each intervention period consisted of 3 days , during which blood glucose homeostasis was assessed under standardized dietary conditions ( supplementary fig .
, subjects reduced their sedentary behavior either by three 15-min bouts of adl ( postmeal strolling ; 3 mets ) or by a single 45-min bout of moderate - intensity cycling exercise ( 6 mets ) .
on day 1 of each intervention period , participants arrived at the laboratory during the afternoon and received a short period of training in the use of the capillary blood sampling method ( glucocard x meter ; arkray , kyoto , japan ) .
subsequently , a continuous glucose - monitoring device ( glucoday s ; a. menarini diagnostics , florence , italy ) was attached as described previously ( 7 ) and participants returned home . on day 2 , participants arrived at the laboratory by car at 0730 h after an overnight fast .
an intravenous catheter was inserted into an antecubital vein for blood sampling purposes , and participants were equipped with a heart rate monitor ( polar rs300 ) .
participants received breakfast , lunch , and dinner at 0830 , 1230 , and 1700 h , respectively .
blood samples were collected in prechilled edta - coated tubes 5 min before each meal , and 90 and 150 min after each meal ( total of 9 samples ) .
after the last blood sample at 1930 h subjects went home . on day 3 , subjects reported back to the laboratory during the morning for removal of the continuous glucose - monitoring device .
the three intervention periods were identical with the exception of the three 15-min bouts of adl or the single 45-min bout of endurance - type exercise , which were performed on the second day of the experimental period ( supplementary fig .
participants were restricted to a sedentary laboratory environment and spent the day seated in a chair or couch while reading , talking , viewing television , or working on a laptop computer . during the adl condition , sedentary behavior of the patients
was interrupted by three 15-min bouts of slow - paced strolling ( 3 met ) performed after each main meal ( 0915 , 1315 , and 1745 h ) .
the bouts of strolling were chosen to reflect light adl , such as walking the dog , household tasks , light gardening work , and so on . during each bout ,
the bouts were supervised by a physical therapist . during the exercise condition , participants completed a single 45-min bout of moderate - intensity endurance - type exercise after breakfast ( 0915 to 1000 h ) and were sedentary throughout the remainder of the day . the moderate - intensity exercise bout consisted of continuous cycling performed on a cycle ergometer at 50% of individual maximal workload capacity of the patients , translating to an average absolute workload of 85  4 w ( 6 met ) .
the met values for both the adl and moderate - intensity exercise intervention were estimated based on the compendium of physical activities ( 25 ) .
glucose - lowering medications used by patients are listed in table 1 . except for the screening , intake of all medication
was continued throughout the entire study , including the days that subjects visited the laboratory for testing .
all subjects were asked to maintain their normal physical activity patterns throughout the study but to refrain from exhaustive physical labor and exercise training for 2 days before each experimental period . on day 1 of each intervention period ,
subjects were provided with a standardized dinner and evening snack . on day 2 of each intervention period , the actual test day , participants were served a standardized breakfast ( 0830 h ) , lunch ( 1230 h ) , and dinner ( 1700 h ) in the laboratory , and they consumed a standardized evening snack at home ( 2030 h ) . on day 3 of each intervention period , participants remained fasted until 0830 h ( end of data collection ) , after which they resumed their regular diets .
participant characteristics breakfast and lunch were identical and both meals consisted of bread , margarine , salami slices , cheese , jam , and semi - skim ( 1.5% fat ) milk .
the quantity of the diet ( energy content ) was based on individual daily energy requirements of the patients as calculated with the harris and benedict equation multiplied by a physical activity level value of 1.4 .
the resulting diet provided 9.8  0.1 mj / day , consisting of 50% of energy from carbohydrate , 15% from protein , and 35% from fat .
venous blood samples were collected in prechilled edta - coated tubes and centrifuged at 1,000 g and 4c for 10 min .
aliquots of plasma were immediately frozen in liquid nitrogen and stored at 80c until analyses .
plasma insulin concentrations were determined by a radioimmunoassay for human insulin ( hi-14k ; millipore , ma ) .
whole blood hba1c content was determined in 3 ml venous blood samples by high - performance liquid chromatography ( bio - rad diamat , munich , germany ) .
the data obtained by the continuous glucose monitor were downloaded to a personal computer with glucoday software ( version 3.2.2 ) .
values reported by the continuous glucose - monitoring device were converted into glucose values using the self - monitored capillary blood glucose values , which were obtained at least before each main meal and before night time .
the 24-h glycemic control was derived from the glycemic profiles obtained between 8:30 and 8:30 h on days 2 and 3 of each experimental period and was defined as the average 24-h blood glucose concentration and the prevalence of hyperglycemia over the course of 24 h. based on the american diabetes association / european association for the study of diabetes guidelines for glycemic control ( 26,27 ) , the prevalence of hyperglycemia was defined as total time during which glucose concentrations exceeded 10 mmol / l .
the continuously monitored glycemic profiles obtained on day 2 of each experimental period also were used to assess postprandial glycemic control .
postprandial glycemic control was defined as the incremental area under the curve ( iauc ) above fasting glucose assessed over the 3.5-h postprandial periods after each main meal ( 3.5-h glucose iauc ) , and as the cumulative glucose iauc of all main meals ( 10.5-h glucose iauc ) .
plasma insulin concentrations obtained on day 2 of each experimental period ( from 0830 to 1930 h ) were used to calculate the iauc above fasting plasma insulin by the trapezoidal rule ( 11-h insulin iauc ) .
differences between nontime - dependent variables ( prevalence of hyperglycemia , 24-h glucose concentration , iauc ) were assessed by one - way repeated - measures anova , with treatment as the within - subject factor . in case of a significant main effect ,
pairwise comparisons with bonferroni correction were applied to locate differences between treatments . a two - way repeated - measures anova with time and treatment as within - subject factors was used to compare differences between treatments over time ( plasma insulin concentrations ) . in case of interaction between significant time and treatment , pairwise comparisons with bonferroni correction were applied for separate time points to locate differences between treatments .
all statistical calculations were performed using the spss 15.0.1.1 software package . unless otherwise indicated , data are reported as means  sem .
average heart rates of subjects that were measured between 0830 and 1930 h were 72  2 bpm during the sedentary control condition and 78  2 and 82  2 bpm during the adl ( p < 0.001 ) and exercise conditions ( p < 0.001 ) , respectively .
as expected , 45 min of moderate - intensity exercise ( 125  4 bpm ) was more intense than the 45 min of adl ( 100  2 bpm ; p < 0.001 ) .
mmol / l ) was prevalent for 6 h 51 min ( 1 h 4 min ) throughout the day .
a single bout of moderate - intensity exercise significantly reduced the daily prevalence of hyperglycemia to 4 h 47 min  1:02 ( 34  7% ; p < 0.001 ; fig .
although blood glucose concentrations were clearly attenuated after the onset of the 15-min bouts of adl ( fig .
1 ) , the prevalence of hyperglycemia over the course of 24 h was not significantly reduced ( 6 h 2 min  1 h 16 min ; p = 0.67 ) .
2b ) . a single session of moderate - intensity exercise significantly reduced average blood glucose concentrations by 0.6  0.1 mmol / l ( p < 0.001 ) relative to the sedentary control condition , whereas average 24-h blood glucose concentrations were basically unchanged during the adl condition ( 0.2  0.1 mmol / l ; p = 0.92 ) .
the 24-h glycemic profiles in type 2 diabetic patients under sedentary conditions and under conditions in which prolonged sedentary time was reduced by three 15-min bouts of activities of daily living ( adl ; gray squares ) or by a single 45-min bout of moderate - intensity endurance - type exercise ( checkered square ) .
the dotted lines indicate the ingestion of the main meals ( 0830 , 1230 , and 1700 h ) or snack ( 2030 h ) .
the error bars are not shown for clarity . the impact of the three experimental conditions on the prevalence of hyperglycemia over the course of 24 h ( a ) , average 24-h blood glucose concentrations ( b ) , and the cumulative postprandial glucose iauc ( c ) .
moderate - intensity exercise strongly reduced the glycemic response to breakfast and , to a lesser extent , the glycemic response to lunch and dinner ( p < 0.05 for all postprandial periods ; supplementary table 1 ) .
the decrements in postprandial glucose concentrations observed in the adl condition did not reach statistical significance for any of the postprandial periods ( p > 0.05 ; supplementary table 1 ) .
more important , however , the cumulative glucose response to breakfast , lunch , and dinner ( 10.5-h iauc ; fig .
2c ) was significantly lower during both the adl ( 365  51 mmol / l/10.5 h ; p < 0.05 ) and moderate - intensity exercise condition ( 285  38 mmol / l/10.5 h ; p < 0.001 ) compared with the sedentary control condition ( 448  54 mmol / l/10.5 h ) .
the 35  5% reduction in the cumulative glucose iauc during the moderate - intensity exercise condition was greater than the 17  6% reduction observed in the adl condition , although this observation did not reach statistical significance ( p = 0.06 ) .
the postprandial increments in plasma insulin concentrations were significantly attenuated when adl were performed in the early postprandial phase . a single bout of moderate - intensity exercise soon after breakfast blunted the subsequent increase in plasma insulin concentrations .
the impact of moderate - intensity exercise on plasma insulin concentrations persisted for up to several hours after exercise .
the resulting plasma insulin response to the standardized diet ( 11-h positive iauc ) was 17  5% lower during the adl condition ( 214  24 nmol / l/11 h ; p < 0.05 ) and 33  4% lower during the exercise condition ( 170  18 nmol / l/11 h ; p < 0.001 ) relative to the sedentary control condition ( 250  23 nmol / l/11 h ) .
the insulin iauc during the exercise condition also was lower compared with the adl condition ( p < 0.001 ) . the impact of the three experimental conditions on insulin concentrations over time ( a ) and the postprandial insulin iauc ( b ) .
average heart rates of subjects that were measured between 0830 and 1930 h were 72  2 bpm during the sedentary control condition and 78  2 and 82  2 bpm during the adl ( p < 0.001 ) and exercise conditions ( p < 0.001 ) , respectively .
as expected , 45 min of moderate - intensity exercise ( 125  4 bpm ) was more intense than the 45 min of adl ( 100  2 bpm ; p < 0.001 ) .
mmol / l ) was prevalent for 6 h 51 min ( 1 h 4 min ) throughout the day .
a single bout of moderate - intensity exercise significantly reduced the daily prevalence of hyperglycemia to 4 h 47 min  1:02 ( 34  7% ; p < 0.001 ; fig .
although blood glucose concentrations were clearly attenuated after the onset of the 15-min bouts of adl ( fig .
1 ) , the prevalence of hyperglycemia over the course of 24 h was not significantly reduced ( 6 h 2 min  1 h 16 min ; p = 0.67 ) .
2b ) . a single session of moderate - intensity exercise significantly reduced average blood glucose concentrations by 0.6  0.1 mmol / l ( p < 0.001 ) relative to the sedentary control condition , whereas average 24-h blood glucose concentrations were basically unchanged during the adl condition ( 0.2  0.1 mmol / l ; p = 0.92 ) . the 24-h glycemic profiles in type 2 diabetic patients under sedentary conditions and under conditions in which prolonged sedentary time was reduced by three 15-min bouts of activities of daily living ( adl ; gray squares ) or by a single 45-min bout of moderate - intensity endurance - type exercise ( checkered square ) .
the dotted lines indicate the ingestion of the main meals ( 0830 , 1230 , and 1700 h ) or snack ( 2030 h ) .
the error bars are not shown for clarity . the impact of the three experimental conditions on the prevalence of hyperglycemia over the course of 24 h ( a ) , average 24-h blood glucose concentrations ( b ) , and the cumulative postprandial glucose iauc ( c ) .
moderate - intensity exercise strongly reduced the glycemic response to breakfast and , to a lesser extent , the glycemic response to lunch and dinner ( p < 0.05 for all postprandial periods ; supplementary table 1 ) .
the decrements in postprandial glucose concentrations observed in the adl condition did not reach statistical significance for any of the postprandial periods ( p > 0.05 ; supplementary table 1 ) .
more important , however , the cumulative glucose response to breakfast , lunch , and dinner ( 10.5-h iauc ; fig .
2c ) was significantly lower during both the adl ( 365  51 mmol / l/10.5 h ; p < 0.05 ) and moderate - intensity exercise condition ( 285  38 mmol / l/10.5 h ; p < 0.001 ) compared with the sedentary control condition ( 448  54 mmol / l/10.5 h ) .
the 35  5% reduction in the cumulative glucose iauc during the moderate - intensity exercise condition was greater than the 17  6% reduction observed in the adl condition , although this observation did not reach statistical significance ( p = 0.06 ) .
the postprandial increments in plasma insulin concentrations were significantly attenuated when adl were performed in the early postprandial phase . a single bout of moderate - intensity exercise soon after breakfast blunted the subsequent increase in plasma insulin concentrations .
the impact of moderate - intensity exercise on plasma insulin concentrations persisted for up to several hours after exercise .
the resulting plasma insulin response to the standardized diet ( 11-h positive iauc ) was 17  5% lower during the adl condition ( 214  24 nmol / l/11 h ; p < 0.05 ) and 33  4% lower during the exercise condition ( 170  18 nmol / l/11 h ; p < 0.001 ) relative to the sedentary control condition ( 250  23 nmol / l/11 h ) .
the insulin iauc during the exercise condition also was lower compared with the adl condition ( p < 0.001 ) . the impact of the three experimental conditions on insulin concentrations over time ( a ) and the postprandial insulin iauc ( b ) .
the current study demonstrated that hyperglycemia is highly prevalent throughout the day in patients with type 2 diabetes .
a single 45-min bout of endurance - type exercise strongly reduced the prevalence of hyperglycemia over the course of 24 h. an increase in adl ( three 15-min bouts of postmeal strolling ) improved postprandial blood glucose homeostasis but did not significantly reduce the prevalence of hyperglycemia over 24 h. elevated blood glucose concentrations , which are most evident in the postprandial state , increase the risk for diabetes complications and mortality ( 16 ) . in the current study , type 2 diabetic patients exceeded the recommended upper limit for postprandial glucose concentrations of 10
strikingly , these hyperglycemic episodes occurred despite the fact that most patients were using oral blood glucose  lowering medication and were relatively well controlled according to the hba1c level ( 6.9  0.1% [ 52  1 mmol / mol ] ; table 1 ) .
these findings are in agreement with previous observations of the prevalence of hyperglycemia ( 8,28 ) and emphasize the need for additional treatment strategies in type 2 diabetes . although the acute impact of moderate - intensity to high - intensity exercise on 24-h blood glucose homeostasis has been well established ( 1218 ) , recent data also suggest an important role for light physical activity in maintaining or restoring proper glycemic control ( 2123 ) . thus far , experimental studies investigating the impact of such light physical activity strategies on glycemic control in type 2 diabetic patients are lacking .
the current study shows that the introduction of repeated bouts of adl during sedentary behavior represents an effective interventional strategy to attenuate the increase in blood glucose and insulin concentrations after sequential meals in type 2 diabetic patients ( figs .
, these findings confirm our hypothesis that simply performing adl can improve postprandial blood glucose homeostasis .
considering the fact that postprandial hyperglycemia is a strong and independent risk factor for cardiovascular morbidity and mortality ( 46 ) , our data indicate that even adl can improve cardiovascular risk profiles in patients with type 2 diabetes .
hence , the beneficial impact of such light physical activity on postprandial glucose concentrations may , at least to some extent , explain the relationships observed between active and sedentary behavior and cardiovascular morbidity and mortality ( 2932 ) . the exact mechanisms by which light physical activity reduces postprandial blood glucose concentrations remain to be explored . nonetheless , the concomitant reduction in postprandial insulin concentrations as observed in the current study ( fig .
3 ) suggests an important role for noninsulin - dependent ( i.e. , contraction - induced ) glucose disposal .
it also could be speculated that delayed gastrointestinal transit or splanchnic hypoperfusion attributable to the physical activity might have contributed to the lowered postprandial glycemic and insulinemic responses ( 33 ) .
however , this effect is likely to be small considering the low intensity of the adl intervention . despite its positive effects on postprandial glucose and insulin concentrations
, the adl intervention did not significantly reduce the prevalence of hyperglycemic blood glucose excursions over the entire 24-h period ( fig .
in contrast , a single 45-min bout of moderate - intensity endurance - type exercise was shown to reduce the daily prevalence of hyperglycemia by 34% , along with a reduction of 0.6 mmol / l in average 24-h blood glucose concentrations ( fig .
2a and b ) . given the tight relationship between average 24-h glucose concentrations and hba1c ( 34 ) , the moderate - intensity endurance - type exercise intervention is more likely to affect long - term glycemic control ( i.e. , hba1c ) than the adl intervention .
moreover , the reductions in postprandial glucose and insulin iauc induced by moderate - intensity exercise ( 35 and 33% , respectively ) were twofold greater than the decrements observed during the adl condition ( 17% for both glucose and insulin iauc ) .
thus , when matched for total activity duration , a single bout of moderate - intensity endurance - type exercise has a much greater impact on blood glucose homeostasis than repeated bouts of low - intense adl .
we previously have speculated that the total volume of exercise ( i.e. , product of frequency , duration , and intensity ) is of prime importance with respect to glycemic control ( 15 ) .
this theory also could explain the greater blood glucose  lowering effects observed in the endurance - type exercise condition as opposed to the adl condition .
the total energy expended during the 45 min of moderate - intensity endurance - type exercise ( 350 kcal ) was approximately twofold higher compared with the 45 min ( three 15-min bouts ) of adl ( 175 kcal ) .
interestingly , this difference in volume tends to be in agreement with the twofold greater decline in postprandial glucose and insulin iauc observed during the exercise as opposed to the adl condition .
response relationship between the volume of aerobic physical activity and the subsequent improvements in blood glucose homeostasis .
response relationship also has been observed recently between the volume of exercise training and improvements in insulin sensitivity ( 35 ) .
moreover , for a fixed volume of exercise , high - intensity exercise was not found to be more effective than moderate - intensity in improving long - term glycemic control ( i.e. , hba1c ) ( 36,37 ) .
taken together , it is tempting to speculate that equal volumes of adl and moderate - intensity exercise lead to similar improvements in glycemic control .
this also would provide an answer to the question of whether an increase in adl provides any surplus benefit for those type 2 diabetic patients who already perform substantial levels of physical activity during daily life . in conclusion ,
implementation of moderate - intensity endurance - type exercise or repeated bouts of adl markedly improve postprandial blood glucose handling in type 2 diabetic patients .
when matched for total duration , a single bout of moderate - intensity endurance - type exercise has a greater impact on daily blood glucose homeostasis than repeated bouts of adl .
nonetheless , the introduction of repeated adl bouts during prolonged sedentary behavior forms a valuable strategy in the management of blood glucose homeostasis in type 2 diabetes , especially in those patients who are unable or reluctant to perform structured exercise .
 objectiveto investigate the impact of activities of daily living ( adl ) versus moderate - intensity endurance - type exercise on 24-h glycemic control in patients with type 2 diabetes.research design and methodstwenty males with type 2 diabetes participated in a randomized crossover study consisting of three experimental periods of 3 days each . 
 subjects were studied under sedentary control conditions , and under conditions in which prolonged sedentary time was reduced either by three 15-min bouts of adl ( postmeal strolling , 3 mets ) or by a single 45-min bout of moderate - intensity endurance - type exercise ( 6 mets ) . 
 blood glucose concentrations were assessed by continuous glucose monitoring , and plasma insulin concentrations were determined in frequently sampled venous blood samples.resultshyperglycemia ( glucose > 10 
 mmol / l ) was experienced for 6 h 51 min 1 h 4 min per day during the sedentary control condition and was significantly reduced by exercise ( 4 h 47 min  1 h 2 min ; p < 0.001 ) , but not by adl ( 6 h 2 min  1 h 16 min ; p = 0.67 ) . 
 the cumulative glucose incremental areas under the curve ( aucs ) of breakfast , lunch , and dinner were , respectively , 35  5% ( p < 0.001 ) and 17  6% ( p < 0.05 ) lower during the exercise and adl conditions compared with the sedentary condition . 
 the insulin incremental aucs were , respectively , 33  4% ( p < 0.001 ) and 17  5% ( p 
 < 0.05 ) lower during the exercise and adl conditions compared with the sedentary condition.conclusionswhen matched for total duration , moderate - intensity endurance - type exercise represents a more effective strategy to improve daily blood glucose homeostasis than repeated bouts of adl . 
 nevertheless , the introduction of repeated bouts of adl during prolonged sedentary behavior forms a valuable strategy to improve postprandial glucose handling in patients with type 2 diabetes . 