the tilting provokes blood pooling in the splanchnic and the lower extremity circulation which triggers the neural and humoral compensatory mechanisms aiming to maintain stable blood pressure and cerebral perfusion .
then the diagnostic utility of the tilt test has been expanded for diagnosis of orthostatic hypotension , autonomic failure , postural tachycardia syndrome , and other forms of orthostatic intolerance .
common orthostatic symptoms are usually defined by heart rate and blood pressure responses to tilting .
the orthostatic cerebral blood flow is less studied ; even many orthostatic symptoms are due to orthostatic cerebral hypoperfusion .
therefore , the purpose of this study was to analyze the cerebral blood flow and cardiovascular patterns in common orthostatic syndromes .
the study was approved by the institutional review board of the university of massachusetts medical school as a minimal risk study .
this retrospective , single - center study included patients with history of autonomic function testing at the university of massachusetts medical school , autonomic laboratory , between years 2007 and 2014 .
the first group constitutes orthostatic intolerance symptoms which include dizziness , lightheadedness , unexplained falls , chronic fatigue , heaviness , chest pain , and shortness of breath .
the second group includes patients with history of unexplained loss of consciousness that were referred for evaluation of suspected syncope .
only the tilt test results are reported here . after a resting supine period of at least 10 minutes duration
, subjects were tilted at 70 degrees for 10 minutes or longer if patients can tolerate . during the whole testing ,
heart rate , blood pressure , respiratory movement , and cerebral blood flow velocity ( cbfv ) were monitored .
arterial blood pressure was measured in the upper arm intermittently using an automated noninvasive oscillometric device dinamap procare monitor 100 ( ge , fairfield , ct ) , as well as continuously by infrared finger plethysmography ( finapress medical systems , amsterdam , netherlands ) .
nasal thermistor and end tidal co2 ( capstar-100 , cwe , inc . , ardmore , pa ) were used for monitoring respiratory parameters .
cbfv was monitored using transcranial doppler ( multidop t , dwl , singen , germany ) with a 2  mhz probe .
cbfv was obtained from the left middle cerebral artery with the doppler probe attached to a three - dimensional positioner which kept the probe at a constant depth and angle of insonation .
. normally heart rate increases at least 10 beats per minute ( bpm ) but less than 30  bmp during the tilt ( table 1 ) [ 3 , 6 ] .
the normal response is a drop of systolic blood pressure < 20  mmhg and diastolic blood pressure < 10  mmhg on upright posture .
since the baseline cbfv is gender and age dependent , the orthostatic criteria are given in relative values where the supine baseline is 100% .
the threshold for normal drop of the mean cbfv is 90% ( 1st minute ) , 89% ( 5th minute ) , and 85% ( 10th minute ) of the supine baseline ( = 100% ) which immediately precedes the tilt .
pots is defined by the presence of orthostatic symptoms associated with an increment of heart rate by 30  bpm held for more than 30 seconds when changing position from supine to upright in the absence of orthostatic hypotension .
the timing of heart rate increment is either not mentioned   or defined within 5 minutes   or within 10 minutes or longer   of the upright posture . early description of pots also required the absolute heart rate 120  bpm or more , while the heart rate below 120 during the tilt test was termed as mild orthostatic intolerance .
to avoid overlap with the definition of the inappropriate sinus tachycardia ( see below ) , an additional criterion for the pots is average heart rate < 100  bpm during supine baseline before the tilting .
the cbfv may be abnormally decreased during tilting due to hyperventilation induced hypocapnia during the tilt .
historically , oh is defined as a drop 20  mmhg in systolic blood pressure or 10  mmhg in diastolic blood pressure within three minutes of standing or head - up tilt .
this definition does not take into account the baseline value ; therefore a modified definition was that using a relative drop of 80% and 85% in systolic and mean blood pressure , respectively , where supine baseline is 100% .
ohtn is a common syndrome with a prevalence of 1.1%   and occurs in 16.3% of older hypertensive patients .
ohtn is defined as a postural increase of systolic blood pressure by at least 20  mmhg   or an increase in systolic blood pressure during the tilt to 120% and above where supine baseline is equal to 100% .
the latter definition was used in this study since it accounts for a supine baseline .
neurally mediated , also called reflex , neurocardiogenic , or vasovagal , syncope is a syncope triggered by a neural reflex resulting in systemic hypotension , reduced cardiac output , peripheral vasodilatation , and/or bradycardia .
based on the heart rate responses , syncope can be divided into three forms : cardioinhibitory , vasodepressor , and mixed which is the most common . in general , the vasodepressor syncope is due to a predominant loss of upright vasoconstrictor tone .
bradycardia or asystole predominates in cardioinhibitory syncope and both mechanisms ( vasodilatation and cardioinhibition ) are present in mixed syncope .
cbfv has characteristic pattern during syncope consistent with cerebral vasodilation as indicted by increased systolic and decreased diastolic cbfv and thus increased pulsatility index defined as systolic cbfv 
the vasis ( vasovagal syncope international study ) classification   of syncope has been expanded using the cbfv and blood pressure criteria ( table 1 ) . in psychogenic syncope , also termed psychogenic pseudosyncope , consciousness is only apparently lost and global cerebral hypoperfusion is absent . in this study ,
the psychogenic syncope was defined as an apparent loss of consciousness without changes in cbfv indicative of syncope .
pst can be frequently encountered during the tilt test and may be due to underlying anxiety disorder .
paroxysmal pst in this study was defined as intermittent ( duration < 2 minutes ) tachycardia ( heart rate > 100  bpm ) associated with heart rate increment 30  bpm .
ist is associated with persistent or recurrent elevated heart rate ( > 100  bmp ) at rest including supine and excessive or inappropriate heart rate increment in upright position [ 7 , 18 ] . in this
study the  inappropriate  heart rate increment was defined as 30  bpm during the tilt .
ochos is a recently described syndrome associated with reduced orthostatic cerebral blood flow velocity ( cbfv ) without oh , bradycardia , and excessive tachycardia .
the normal supine cbfv is age and gender dependent and pcaf is defined as cbfv less than the lower limit of the normal range where the normal limit is 72.090.38    age 
cm / s and 82.20.45    age  cm / s for men and women , respectively , using the multidop t device ( table 1 ) .
since this pattern ( e.g. , low cbfv and normal or high bp ) may indicate cerebral vasoconstriction , cerebral vascular resistance defined by mean blood pressure / mean cbfv   has been calculated as well .
it is a policy at our autonomic laboratory to acquire blood pressure by both methods , for example , oscillometric and plethysmographic , and if the sustained difference is more than 10% , then finger device should be repositioned .
in the case the difference persists , blood pressure from plethysmographic device should be recalibrated using the oscillometric device .
the syndromes described above were detected automatically by an algorithm written in matlab programming language ( mathworks , natick , ma ) .
the software used in this study is an extension of the quantitative scale for grading of cardiovascular autonomic reflex tests and small fibers from skin biopsies ( qasat ) , also written in matlab .
qasat is an objective and validated instrument for grading of tilt responses which defines normal and abnormal responses in heart rate , blood pressure , and cbfv during the tilt test .
the clinical variables associated with orthostatic syndromes were compared with normal responses using wilcoxon rank test since most of the data had nonnormal distribution .
nine clinical syndromes were compared with the normal response to the tilt , for example , oh , ochos , ohtn , pots , ist , pst , syncope , psychogenic syncope , and pcaf , and therefore the initial significance 0.05 was adjusted by bonferroni correction to 0.005 ( 0.05/10 comparisons ) .
all statistical analyses were performed using jmp 12.0 ( cary , nc ) statistical software .
all patients had unrevealing standardized evaluation including medical history , neurological examination , basic metabolic panel , 12-lead ecg , imaging studies ( ct or mri of the brain ) , and eeg if patients were referred for evaluation of syncope .
669 patients were referred for evaluation of orthostatic symptoms including dizziness and 75 patients were referred for evaluation of unexplained loss of consciousness with a suspected diagnosis of syncope .
however , from these subjects , only 7 subjects had normal entire autonomic testing ( e.g. , qasat total score = 0 ) .
remaining subjects had at least one abnormality in parasympathetic ( evaluated by deep breathing test ) or sudomotor ( evaluated by qsart ) functions . from 669 patients evaluated for orthostatic intolerance ( not syncope ) ,
the tilt test was normal in 77 patients . from 75 patients referred for evaluation of syncope , the tilt test showed normal response in 25 subjects ,
neutrally mediated syncope in 33 patients , and abnormal results but nonsyncope pattern in remaining patients ( table 2 ) .
additional 23 patients that had syncope during the tilt test were referred for nonsyncope evaluation . 

table 2 shows details of orthostatic symptoms in each syndrome including the frequency of reproduction of previous symptoms that prompted autonomic testing and the frequency of a new diagnosis obtained from the tilt test .
the symptoms were commonly reproduced in the pst ( 100% ) , pots ( 99% ) , ochos ( 98% ) , ist ( 86% ) , and uncompensated oh ( 85% ) .
most common new diagnosis was obtained from the tilt test , which means the diagnosis was not mentioned in the chart or was not mentioned in the reason for the referral of the testing , which was ochos ( n = 97 ) , pcaf ( n = 67 ) , and pots ( n = 67 ) .
figure 1 shows head - to - head comparisons of normal response , oh and ochos .  
figure 2 compares the profile of common tachycardia syndromes including pots , ist , and pst .  
figure 3 shows three main types of syncope , for example , cardiovagal , vasodepressor , and mixed .
pure syncope pattern with otherwise normal responses on the tilt test was detected in 32 patients .
syncope was combined with other syndromes including pots ( 12 patients ) , ohtn ( 1 patient ) , ist ( 3 patients ) , pst ( 3 patient ) , and pcaf ( 1 patient ) .
characteristic patterns of syncope are seen in figure 3 with details in figures 811 and 17 .
all three types of syncope share common pattern in cbfv which is consistent with cerebral vasodilation ( figures 3 and 811 ) .
oh can be progressive ( figures 1 , 4 , and 7 ) , transient ( figure 5 ) , associated with stable orthostatic cbfv , for example , compensated ( figures 4 and 5 ) , or reduced orthostatic cbfv , for example , uncompensated ( figures 6 and 7 ) .
mean blood pressure was reduced in both oh groups while the cbfv tilt drop score was abnormal in the oh - uncompensated group during the tilt ( table 2 ) .
ochos ( figures 1 , 12 , and 20 ) showed primary reduction in orthostatic cbfv with normal orthostatic heart rate responses and without orthostatic hypotension . in ohtn ( figure 13 ) orthostatic blood pressure was elevated while orthostatic cbfv was stable .
characteristic features of tachycardia syndromes ( pots , ist , and pst ) are shown in figures 2 , 1518 , and 23 and table 2 .
both pots and pst patients were younger than subjects with the normal tilt test and had elevated heart rate during the tilt .
ist subjects were younger than subjects with the normal tilt response and had elevated heart rate in supine position and during the tilt .
vasomotor oscillations ( figure 19 ) , spurious fluctuations of blood pressure ( figure 20 ) , and sustained drift in blood pressure ( figure 21 ) obtained from finger plethysmographic device can be recognized by simultaneous recording of the arm blood pressure and cbfv .
eight patients had pseudosyncope ( figures 22 - 23 ) with stable orthostatic cbfv . from these patients ,
tilt test showed normal responses in 4 subjects , pots in 2 subjects , and oh - compensated and mixed syncope in 1 subject .
patients with pcaf had reduced cbfv in the supine position , had elevated cerebral vascular resistance in supine position and during the tilt , and had abnormal drop of cbfv score during the tilt ( table 2 ) . 

table 3 summarizes the main diagnostic features of common patterns encountered during the tilt test .
characteristic pattern associated with a particular orthostatic syndrome can be grouped into abnormalities predominantly affecting heart rate ( pst , ist , and pots ) , blood pressure ( syncope , oh , and ohtn ) , and cerebral blood flow ( ochos , pcaf ) .
psychogenic pseudosyncope is associated with stable cbfv without any particular heart rate or blood pressure pattern .
this study also showed that criteria for several orthostatic syndromes had to be modified to allow unambiguous pattern classifications .
orthostatic symptoms , except of syncope , are nonspecific and in general poorly correlating with orthostatic blood pressure or heart rate .
this study showed that orthostatic drop in cbfv is more sensitive and specific marker of prediction of orthostatic symptoms than orthostatic blood pressure changes .
criteria for orthostatic syndromes are heterogeneous ; some criteria are exclusively physiological ( e.g. , oh which can be symptomatic or asymptomatic ) while the others require also the presence of symptoms , for example , pots .
nevertheless , the information obtained from the tilt test should not be used in isolation but always on clinical ground .
primarily , it is the timing of tachycardia that distinguishes each syndrome . using the nonmodified criteria , majority of patients with ist and pst
the ambiguities in determination of tachycardia syndromes were solved by modifying the diagnostic criteria as mentioned in the section 2 .
cbfv is characteristically reduced in pots , normal in ist , and normal or transiently elevated in pst .
loss of consciousness usually occurs if systolic blood pressure declines to 60  mmhg   that is accompanied by cerebral vasodilation . in this study ,
the diastolic cbfv was close or equal to zero , the mean cbfv was less than 30  cm / sec , and pulsatility index increased which is consistent with cerebral vasodilation . it is advantageous to use cbfv in differentiation of pseudosyncope or spurious decline in finger blood pressure ; in both conditions cbfv remains stable .
pcaf is defined as low supine cbfv without supine hypotension or other hemodynamic abnormalities that can explain low cbfv .
patients with pcaf have increased cerebral vascular resistance that may be due to cerebral autoregulatory failure .
cbfv is also reduced during the tilt in spite of stable orthostatic blood pressure , further pointing to altered cerebral autoregulation .
this hypothesis is supported by a previous study that showed significant negative correlation between cbfv and severity of white matter abnormalities in the mri which is a marker of small vessel disease .
clinical significance of pcaf is unclear but pcaf may be a cause of cerebral dysfunction due to chronic cerebral hypoperfusion .
original definition of ohtn included only an increase of blood pressure during the tilt while heart rate and cbfv responses to tilting were not considered .
these limitations can cause diagnostic overlap with pots since occasional rise in blood pressure during tilting can be observed in the hyperadrenergic form of pots .
consider an example in figure 12 , where the excessive rise in blood pressure is associated with drop in cbfv during the tilt test .
this patient has diagnosis of ochos , but using the original ohtn criteria he also qualifies for diagnosis of ohtn .
the above mentioned shortcomings were overcome by including the heart rate and cbfv responses in the ohtn definition ( table 1 ) .
typically , the tilt test uses heart rate and blood pressure responses in classification of results .
the usefulness of an additional cbfv monitoring during the tilt test can be demonstrated by comparing the yield of the tilt test with and without cbfv monitoring . without cbfv and when using heart rate and blood pressure monitoring only , then all ochos subjects ( 13% or 97 subjects ) will be labeled as having normal tilt test .
since they have orthostatic symptoms ( 100% of ochos patients were symptomatic in this study ) they may be misdiagnosed as having psychogenic , vestibular , or an unclassified disorder . furthermore 67 ( 9% ) patients with diagnosis of pcaf would also be missed without using cbfv .
cbfv also helps to differentiate pst , ist , and pots and spurious blood pressure oscillations .
no specific changes in hemodynamic parameters except of stable cbfv have been found in this study .
this is in contrast with a recent study   which observed elevated heart rate and blood pressure before and during the loss of consciousness . in this study no particular heart rate and blood pressure pattern
the characteristic drop in cbfv associated with a vasodilatation pattern seen in syncope is missing in pseudosyncope .
nevertheless , the clinical significance of adjusted criteria needs to be validated in future studies . 

table 3 summarizes common patterns associated with the tilt test and emphasizes the main features that enable differentiating each pattern .
tilt test can be used as an add - on in diagnosis of orthostatic syndromes .
however diagnostic criteria for several syndromes had to be made more explicit to allow unambiguous pattern classification . to be able to classify all patterns ,
 
 objective . 
 the head - up tilt test is widely used for evaluation of orthostatic intolerance . 
 although orthostatic symptoms usually reflect cerebral hypoperfusion , the cerebral blood flow velocity ( cbfv ) profile in orthostatic syndromes is not well described . 
 this study evaluated cbfv and cardiovascular patterns associated with the tilt test in common orthostatic syndromes . 
 methods . 
 this retrospective study analyzed the tilt test of patients with history of orthostatic intolerance . 
 the following signals were recorded : ecg , blood pressure , cbfv using transcranial doppler , respiratory signals , and end tidal co2 . results . 
 data from 744 patients were analyzed . 
 characteristic pattern associated with a particular orthostatic syndrome can be grouped into abnormalities predominantly affecting blood pressure ( orthostatic hypotension , orthostatic hypertension syndrome , vasomotor oscillations , and neurally mediated syncope  
 cardioinhibitory , vasodepressor , and mixed ) , cerebral blood flow ( orthostatic hypoperfusion syndrome , primary cerebral autoregulatory failure ) , and heart rate ( tachycardia syndromes : postural tachycardia syndrome , paroxysmal sinus tachycardia , and inappropriate sinus tachycardia ) . 
 psychogenic pseudosyncope is associated with stable cbfv . 
 conclusions . 
 the tilt test is useful add - on in diagnosis of several orthostatic syndromes . 
 however diagnostic criteria for several syndromes had to be modified to allow unambiguous pattern classification . 
 cbfv monitoring in addition to blood pressure and heart rate may increase diagnostic yield of the tilt test . 