earlier studies have reported that either caloric restriction or the inactivation of nutrient - dependent pathways is able to increase life extension in different eukaryotes .
the extension of lifespan in various organisms is associated with increased resistance to oxidative stress ( 1 ) .
the findings of numerous studies using various models of organisms have provided indirect evidence for the hypothesis that reactive oxygen species ( ros ) production and subsequent induction of ros defense are essential contributors to longevity ( 2 ) . as in higher eukaryotes ,
the unicellular yeast , saccharomyces cerevisiae , has been the most used yeast model in aging studies ( 3 , 4 ) .
yeast lifespan can be measured through two methods : replicative aging refers to the number of divisions a single mother cell undergoes before death , whereas chronological lifespan measures the viability of cultures at the stationary phase of the growth curve ( 5 , 6 ) .
the fission yeast , schizosaccharomyces pombe , has been recently used as a model for chronological aging studies inasmuch as it is more similar to the last common ancestor of humans and fungi ( 7 ) .
nutrient restriction increases resistance to oxidative stress , reduces macromolecular damage , and promotes lifespan in s. pombe ( 8 - 10 ) .
roux et al . ( 11 ) revealed that the s. pombe gpa2 mutant exhibits not only a short lifespan but also impaired mitochondrial regulation and high production of ros .
( 12 ) reported that calorie restriction favors oxidative metabolism , ros production , and sty1 map kinase activation and this stress pathway favors lifespan extension . in s. pombe ,
glucose , which is a primary carbon source , is detected by g protein - coupled receptors and generates a signal via the camp - dependent protein kinase a ( pka ) ( 13 , 14 ) . the glucose sensing and signaling pathways have also been found to be involved in metabolic adaptation and cellular response to diverse stress agents ( 15 , 16 ) . in s. pombe , the multistep phosphorelay system and the mitogen - activated protein kinase ( mapk ) pathway govern the transcriptional regulation in response to oxidative stress , which is generated by the accumulation of ros ( 17 ) .
mapk sty1 plays an important role in the regulation of downstream targets through triggering two transcriptional activators , atf1 and pap1 , in oxidative stress response ( 18 ) .
these transcription factors activate or induce the expression of antioxidant genes such as ctt1 ( cytoplasmic catalase ) , gpx1 ( glutathione peroxidase ) , ttr1 ( thioredoxin reductase ) , trx2 ( thioredoxin ) , ntp1 ( neutral trehalase ) , pgr1 ( glutathione reductase ) , and sod1 ( superoxide dismutase , sod ) ( 19 ) .
trehalose (  , -1 , 1-diglucose ) is a storage disaccharide and is present in particularly high concentrations in resting and stressed yeast cells ( 20 ) .
trehalose 6-phosphate ( t6p ) is synthesized in s. pombe from glucose 6-phosphate and udp - glucose by t6p synthetase , encoded by the tps1 gene ( 21 ) , and converted to trehalose by t6p phosphatase , encoded by the tpp1gene ( 22 ) .
the breakdown of trehalose to glucose is catalyzed by the enzyme neutral trehalase , encoded by the ntp1 gene ( 23 ) . generally in yeast ,
the regulation of trehalose synthesis and breakdown is done by camp - dependent phosphorylation mechanisms ( 24 ) .
it has been reported that the ntp1 expression is regulated by the pathway of protein kinase cascade activated under osmotic or oxidative stress ( 23 , 25 ) or by the binding of the transcription factors to elements such as camp - response element under thermal stress ( 26 ) . in the present study , ird5 and ird11
were used to evaluate whether or not trehalose contributes to survival under moderate oxidative stress .
in a previous study , we identified two glucose - repression - resistant mutants , namely ird5 and ird11 ( 27 ) . in the ird11 mutant , the oxidative stress response
is affected by glucose signaling in a manner different from that caused by glucose deprivation ( 28 ) .
the ird5 mutant has a lower caloric intake owing to reduced glucose consumption efficiency ( 29 ) .
the inefficient glucose uptake in ird5 might be a cause of increased oxidative stress response .
accordingly , in the present study , through trehalose accumulation measurement and colony - forming unit ( cfu ) counting , we compared the ird mutants and the wild type to clarify the possible relationship between glucose signaling , oxidative stress response , and lifespan in s. pombe .
in this study , the wild type of s. pombe 
 lindner liquefaciens ( 972 h ) and its relative invertase mutants ( i.e. ird5 and ird11 ) , which are resistant to glucose suppression ( 27 ) , were used . the selective medium consisted of 0.5%
yeast extract and 3% sucrose and 400 g / ml 2-deoxy - d - glucose ( 2-dog ) was developed for the ird mutants .
the strains were cultured in the ye medium containing 3% glucose ( repressed condition ) and 0.5% glucose ( glucose starvation condition ) and the ye medium containing 0.1% glucose plus 3% glycerol ( de - repressed condition ) . all the chemicals were provided by sigma - aldrich , germany .
the exponentially growing s. pombe cells ( wild type and ird5 and ird11 ) under repressed conditions were split into two tubes . in the experimental group , 2 mm hydrogen peroxide ( h2o2 ) ( sigma - aldrich , germany ) was added to the medium , resulting in a mild level of oxidative stress in the s. pombe cells ( 30 ) .
after one hour , both experimental and control cells were removed by centrifugation and washed with sterile distilled water so that their trehalose content could be measured .
chronological lifespan analyses were done on the wild type and the ird mutant cells , grown under repressed and de - repressed conditions for 21 days .
usually one day ( the exponential phase ) after starting the cultures , and this time point was designated as day 0 , measurements were started and continued via sampling at 2-day intervals ( 3rd , 5th , ... , 21st days of the study ) .
this study was performed under three conditions : repressed ; de - repressed ; and stressed conditions . from each group cells ,
samples were collected on the 1st ( the exponential phase ) , 3rd , and 5th ( stationary phase ) days of the study , and trehalose accumulation was measured for each case .
trehalose was extracted and assayed as is described by parrou and francois ( 31 ) .
the amount of the trehalose contents of the samples was measured by treatment with trehalase ( sigma - aldrich , germany ) .
the amount of the generated glucose was determined enzymatically via the glucose oxidase - peroxidase system ( god - pod assay ) using a commercial kit ( fluitest-glu , biocon , germany ) .
statistical comparisons were made using the one - way analysis of variance ( anova ) module of graphpad prism 5 .
in this study , the wild type of s. pombe 
 lindner liquefaciens ( 972 h ) and its relative invertase mutants ( i.e. ird5 and ird11 ) , which are resistant to glucose suppression ( 27 ) , were used . the selective medium consisted of 0.5%
yeast extract and 3% sucrose and 400 g / ml 2-deoxy - d - glucose ( 2-dog ) was developed for the ird mutants .
the strains were cultured in the ye medium containing 3% glucose ( repressed condition ) and 0.5% glucose ( glucose starvation condition ) and the ye medium containing 0.1% glucose plus 3% glycerol ( de - repressed condition ) . all the chemicals were provided by sigma - aldrich , germany .
the exponentially growing s. pombe cells ( wild type and ird5 and ird11 ) under repressed conditions were split into two tubes . in the experimental group , 2 mm hydrogen peroxide ( h2o2 ) ( sigma - aldrich , germany ) was added to the medium , resulting in a mild level of oxidative stress in the s. pombe cells ( 30 ) .
after one hour , both experimental and control cells were removed by centrifugation and washed with sterile distilled water so that their trehalose content could be measured .
chronological lifespan analyses were done on the wild type and the ird mutant cells , grown under repressed and de - repressed conditions for 21 days . usually one day ( the exponential phase ) after starting the cultures , and this time point
was designated as day 0 , measurements were started and continued via sampling at 2-day intervals ( 3rd , 5th , ... , 21st days of the study ) .
this study was performed under three conditions : repressed ; de - repressed ; and stressed conditions . from each group cells ,
samples were collected on the 1st ( the exponential phase ) , 3rd , and 5th ( stationary phase ) days of the study , and trehalose accumulation was measured for each case .
trehalose was extracted and assayed as is described by parrou and francois ( 31 ) .
the amount of the trehalose contents of the samples was measured by treatment with trehalase ( sigma - aldrich , germany ) .
the amount of the generated glucose was determined enzymatically via the glucose oxidase - peroxidase system ( god - pod assay ) using a commercial kit ( fluitest-glu , biocon , germany ) .
statistical comparisons were made using the one - way analysis of variance ( anova ) module of graphpad prism 5 .
of the strains under study , ird5 showed the most longevity in both repressed and de - repressed conditions .
the ird11 mutant had a lower lifespan extension than the wild type under repressed condition , while it had a higher lifespan extension than the wild type under de - repressed condition . on the other hand ,
the lifespan extension of ird11 grown in de - repressed condition was similar to that of ird5 grown in repressed or de - repressed condition ( figure 1 ) .
a , grown in repressed condition ( ye-3% glucose ) ; b , grown in de - repressed condition ( ye-0.1% glucose plus 3% glycerol ) ; ( wt : wild type , r : repressed , dr : de - repressed condition ) . to comprehend the relationship between trehalose concentrations in the cells grown in repressed , de - repressed , and stressed conditions , the level of trehalose was estimated in the wild type and the mutant cells .
the intracellular trehalose concentrations during the period of growth under repressed , de - repressed , and stressed conditions relative to the wild type and mutant cells are shown in figure 2 a , figure 2 b , and figure 2 c , respectively .
the results obtained from trehalose content measurement in the three conditions were evaluated in two ways .
first , each group was evaluated individually and the trehalose contents of the groups on the 3rd and 5th days of the study were compared with those of the 1st day .
second , the trehalose content of each group was compared with that of the wild type . a , grown in repressed condition ( ye-3% glucose ) ; b , grown in repressed condition ( ye-0.1% glucose plus 3% glycerol ) ; c , grown in stressed condition ( 2 mm h2o2 , one hour ) .
the trehalose contents are expressed as mg glucose per 0.2 g of wet mass of the cells .
statistical significance was evaluated by one - way anova ( p < 0.05 - 0,001 ) .
( wt : wild type , r : repressed , dr : de - repressed , hp : oxidative stress conditions ) .
* , * * , * * * : each group was evaluated individually and the trehalose contents of the groups on the 3rd and 5th days of the study were compared with those of the 1st day . + , + + , + + + : trehalose content of each group is compared with that of the wild type .
when compared independently , as is depicted in figure 2 a , except for ird5 under repressed condition , the trehalose content was increased remarkably on the 3rd day of the study .
however , a comparison with the wild type showed a sharp decrease in the trehalose level in the ird mutant . as is evident from figure 2 b , under de - repressed condition
, the level of intracellular trehalose was notably increased in the stationary phase ( 3rd day ) , while no significant changes were detected in either ird5 or in ird11 . under stressed condition
( figure 2 c ) , during the stationary phase ( 3rd day ) , the trehalose level in ird11 was increased as a pattern similar to that observed in the wild type ; nevertheless , only trace amounts of alteration were seen in ird5 .
finally , concerning the wild type during the stationary growth phase , a dramatic decrease was observed in the trehalose content in ird5 ( 3rd and 5th days ) and ird11 ( 3rd day ) .
of the strains under study , ird5 showed the most longevity in both repressed and de - repressed conditions .
the ird11 mutant had a lower lifespan extension than the wild type under repressed condition , while it had a higher lifespan extension than the wild type under de - repressed condition . on the other hand ,
the lifespan extension of ird11 grown in de - repressed condition was similar to that of ird5 grown in repressed or de - repressed condition ( figure 1 ) .
a , grown in repressed condition ( ye-3% glucose ) ; b , grown in de - repressed condition ( ye-0.1% glucose plus 3% glycerol ) ; ( wt : wild type , r : repressed , dr : de - repressed condition ) .
to comprehend the relationship between trehalose concentrations in the cells grown in repressed , de - repressed , and stressed conditions , the level of trehalose was estimated in the wild type and the mutant cells .
the intracellular trehalose concentrations during the period of growth under repressed , de - repressed , and stressed conditions relative to the wild type and mutant cells are shown in figure 2 a , figure 2 b , and figure 2 c , respectively .
the results obtained from trehalose content measurement in the three conditions were evaluated in two ways .
first , each group was evaluated individually and the trehalose contents of the groups on the 3rd and 5th days of the study were compared with those of the 1st day .
second , the trehalose content of each group was compared with that of the wild type . a , grown in repressed condition ( ye-3% glucose ) ; b , grown in repressed condition ( ye-0.1% glucose plus 3% glycerol ) ; c , grown in stressed condition ( 2 mm h2o2 , one hour ) .
the trehalose contents are expressed as mg glucose per 0.2 g of wet mass of the cells .
statistical significance was evaluated by one - way anova ( p < 0.05 - 0,001 ) .
( wt : wild type , r : repressed , dr : de - repressed , hp : oxidative stress conditions ) .
* , * * , * * * : each group was evaluated individually and the trehalose contents of the groups on the 3rd and 5th days of the study were compared with those of the 1st day . + , + + , + + + : trehalose content of each group is compared with that of the wild type .
when compared independently , as is depicted in figure 2 a , except for ird5 under repressed condition , the trehalose content was increased remarkably on the 3rd day of the study . however , a comparison with the wild type showed a sharp decrease in the trehalose level in the ird mutant . as is evident from figure 2 b , under de - repressed condition , the level of intracellular trehalose was notably increased in the stationary phase ( 3rd day ) , while no significant changes were detected in either ird5 or in ird11 . under stressed condition
( figure 2 c ) , during the stationary phase ( 3rd day ) , the trehalose level in ird11 was increased as a pattern similar to that observed in the wild type ; nevertheless , only trace amounts of alteration were seen in ird5 .
finally , concerning the wild type during the stationary growth phase , a dramatic decrease was observed in the trehalose content in ird5 ( 3rd and 5th days ) and ird11 ( 3rd day ) .
the pka activated by glucose starvation and stress - activated mapk pathways regulate the transcription of downstream genes via protein - dna interactions at uas1 and uas2 of the fbp1 gene ( 32 ) .
the existence of cross - talking between sty1p and pka1p regulates not only glucose repression and oxidative stress response pathways but also trehalose accumulation .
( 25 ) reported that the expression of the ntp1 and tps1 genes in s. pombe is partially regulated by the sty1p kinase under salt - induced osmotic stress and conditions of slight oxidative stress and is fully dependent on this kinase under severe oxidative stress .
another study showed that cell viability may depend on capacity to rapidly degrade the trehalose that is accumulated during oxidative stress .
the authors indicated that glutathione reductase can be inhibited by trehalose in a dose - dependent manner . on the other hand , sanchez - fresneda et al .
( 33 ) reported that the stress - induced trehalose accumulation is hog1-independent in candida albicans .  
the extension of lifespan in s. pombe is associated with both calorie restriction and increased resistance to oxidative stress ( 10 - 12 ) .
( 34 ) , our results suggest that lifespan extension in s. pombe may not be associated with oxidative stress resistance and trehalose accumulation .
the oxidative stress - resistant mutant ird11 , which is affected by glucose signaling in a manner different from that caused by glucose deprivation ( 28 ) , had a lower lifespan extension than the wild type under repressed condition and had a higher lifespan extension than the wild type under de - repressed condition .
indeed , the lifespan extension of ird11 grown in de - repressed condition was similar to that of ird5 grown in repressed or de - repressed condition .  
we suggest that the longevity of the ird5 mutant grown in both repressed and de - repressed conditions might be , in part , in consequence of glucose depletion condition caused by a reduced glucose consumption rate in these cells compared to the ird11 mutant and the wild type .
because , contrary to ird11 , there is an adaptive response to oxidative stress in ird5 , caused by glucose sensing / signaling which emerges in glucose starvation ( 29 ) . in light of the results of the present study
, it seems that lifespan extension is mostly related to glucose sensing / signaling rather than oxidative stress response and trehalose accumulation .
 background : glucose is the preferred carbon and energy source in most organisms and plays an active role in the regulation of many biological processes . however , an excess of glucose leads to such undesirable conditions as diabetes and age - related diseases . since schizosaccharomyces pombe homologous of many human genes , it offers several advantages for the investigation of the molecular mechanisms underlying human disease and aging studies . 
 we have identified two glucose - repression - resistant mutants ( ird5 and ird11 ) of s. pombe.objectives:we aimed to investigate the possible relationship between lifespan extension and oxidative stress response induced by exposure to hydrogen peroxide alongside the trehalose accumulation level by using the two s. pombe mutants ( i.e. ird5 and ird11 ) , which are repressed by glucose and are resistant to oxidative stress.materials and methods : we employed trehalose accumulation measurement and colony - forming unit ( cfu ) counting using the ird mutants in exponential and stationary phases and compared them to the wild type grown in repressed , de - repressed , and stressed conditions to clarify the possible relationship between glucose signaling , oxidative stress response , and lifespan in s. pombe.results:the lifespan of the ird5 mutant was significantly longer that of either the ird11 mutant or the wild type cells . under repressed condition 
 , the trehalose content was increased remarkably on the 3rd day of the study in the ird11 mutant and the wild type . under de - repressed condition 
 , the level of intracellular trehalose was notably increased on the 3rd day in ird11 . under stressed condition , the trehalose level in ird11 
 was increased on the 3rd day as a pattern similar to that observed in the wild type.conclusions:our results demonstrated no significant correlation between the ird5 lifespan and the trehalose concentration . 
 likewise , the correlation between lifespan extension , trehalose accumulation , and cellular resistance to hydrogen peroxide was not significant . 