BACKGROUND
intracerebral hemorrhage  is the most debilitating and is associated with the highest mortality rates of all stroke subtypes, has no effective therapeutics, and represents a major public health problem  <cit> .
ich is a multifaceted disorder with acute and chronic mechanisms of injury, including initial mechanical damage due to the expanding hematoma and compression of brain tissue and later inflammatory/oxidative processes primarily resulting from the presence of blood components .
combating the strong neuroinflammatory cascade after ich remains a promising therapeutic target to reduce secondary brain damage and improve patient outcomes  <cit> .
neuroinflammation following brain injury is intimately connected to upregulation of cyclooxygenase  enzymes, mainly the inducible isoform cox- <dig>  coordinated expression of membrane-bound pge synthase- <dig> , and consequent increased production of prostaglandins   <cit> .
prostaglandins have been shown to modulate outcomes in a variety of neurological disorders, including stroke, traumatic brain injury, epilepsy, alzheimer’s disease , and parkinson’s disease , among others .
although selective inhibition of cox- <dig> has been reported to improve outcomes in many preclinical models of these neurological disorders, including ich, the clinical use of these drugs is limited due to the potential for known severe side effects .
therefore, targeting more specific downstream pathways of cox/mpges-mediated prostaglandin signaling may reduce these deleterious side effects while still harnessing the already characterized positive immunomodulatory properties seen with cox- <dig> inhibition.
prostaglandin e <dig>  is a major product of cox/pges activity in the central nervous system and is classically thought of as proinflammatory within the brain  <cit> .
however, emerging evidence suggests a complex scenario with pge <dig> capable of exerting pro- and/or anti-inflammatory functions depending on the underlying neuropathophysiology  <cit> .
the many actions of pge <dig> are produced by its differential stimulation of mainly the g-protein-coupled e prostanoid  receptors 1- <dig>  which display varied anatomical distributions, cellular expression profiles, ligand binding affinity, desensitization kinetics, and signal transduction pathways .
in the present study, we focus on the ep <dig> receptor, which is reported to be expressed in the thalamus/hypothalamus, hippocampus, cortex, striatum, and cerebellum  <cit> .
ep <dig> stimulation results in increased intracellular calcium levels via coupling to a gαq protein, phosphatidyl inositol hydrolysis, and extracellular calcium influx  <cit> .
we and others have documented the pro- and/or anti-inflammatory effects and neurotoxic or neuroprotective properties of signaling through the pge2-ep <dig> axis in various models of acute and chronic neurological disorders such as stroke, hypoxic-ischemic encephalopathy , traumatic brain injury , ad, and pd .
in models of transient forebrain ischemia, focal ischemia, and excitotoxicity, pge2-ep <dig> signaling aggravates brain damage possibly by increasing blood brain barrier  disruption and vascular tone and decreasing neuronal resistance to oxidative stress and cell death  <cit> .
similarly, the neurotoxicity associated with pge2-ep <dig> signaling was shown in a combined model of ad and cerebral ischemia, where amyloid plaques and neuronal damage were reduced in ep1-deleted ad mice following ischemic insult  <cit> .
further, inhibition of ep <dig> with a selective antagonist reduced hie cerebral injury  <cit> .
in a model of toxin-induced parkinsonism, dopaminergic neurons were protected and apomorphine-induced contralateral rotations were decreased in ep1−/− mice  <cit> .
on the contrary, deletion or antagonism of the ep <dig> receptor failed to provide neuroprotection in a contusive model of tbi  <cit> .
last, we have recently shown that genetic deletion of the ep <dig> receptor aggravates ich-induced brain injury and impairs functional recovery, where mechanistic evidence was provided to suggest ep <dig> stimulation positively modulates microglial activation and phagocytosis  <cit> .
thus, signaling through the pge2-ep <dig> axis has a complex role following brain injury and appears to depend on the underlying pathologic processes, and possibly also on the genetic and/or pharmacologic approach used.
the present study aimed to explore our prior data in ep1−/− mice by extension to post-ich pharmacologic manipulation of the ep <dig> receptor.
in line with our previous results, here we show that selective antagonism of the ep <dig> receptor with sc- <dig> chronically following ich exacerbates brain injury and increases ferric iron deposition and microgliosis.
furthermore, repeated administration of 17-pt-pge <dig>  an ep <dig> agonist, after ich improved functional recovery and reduced astrogliosis, leukocyte transendothelial migratory potential, peripheral neutrophil infiltration, and bbb breakdown.
methods
mice
experiments were performed with 11–13-week-old c57bl/ <dig> male mice.
the colony was bred and maintained in our animal facilities in a temperature-controlled reverse light cycle environment  so that behavioral testing could be performed during the awaken phase.
mice were allowed free access to food and water before and after surgical procedures.
all animal protocols were approved by the university of florida institutional animal care and use committee.
ich model
ich was induced by unilateral intrastriatial infusion of collagenase type vii-s  as we have described previously  <cit> .
briefly, mice were anesthetized with isoflurane  and immobilized on a stereotactic frame .
after making a small incision in the skin overlying the skull, a craniotomy was performed  <dig>  mm anterior and  <dig>  mm right of bregma.
collagenase  was infused  <dig>  mm ventral from the dura over a 5-min period using a syringe with a 26-gauge blunt tip needle .
rectal temperatures were maintained at  <dig>  ±  <dig> °c throughout all surgical procedures and mice were allowed to recover in temperature- and humidity-controlled chambers for at least 1 h postoperatively.
experimental groups and drug treatments
mice  were equally distributed into three treatment groups: antagonist, agonist, and control.
the antagonist 8-chloro-2-hydrazide-dibenz <cit> oxazepine-10-carboxylic acid, monohydrochloride]  is selective for the ep <dig> receptor  and was administered at a dose of 10 µg/kg of body weight.
intraperitoneal treatment with this dose either 5 min or 6 h after reperfusion significantly reduced infarct volume and brain swelling and improved neurological deficits in the transient middle cerebral artery occlusion stroke model  <cit> .
the agonist 9-oxo-11α,15s-dihydroxy-17-phenyl- <dig> ,20-trinor-prosta-5z,13e-dien-1-oic acid  is non-selective for the ep <dig> receptor, with ki values of  <dig>  and  <dig>  nm for the ep <dig> and ep <dig> receptors, respectively  <cit> .
the dose of  <dig>  mg/kg of body weight was chosen based on a previous study where intravenous pretreatment  was shown to aggravate histamine-mediated gastric mucosal injury  <cit> .
sc- <dig> and 17-pt-pge <dig> were purchased from cayman chemicals .
stock solutions were prepared in dimethyl sulfoxide  and subsequently aliquoted and stored at −20°c.
solutions for injections were freshly diluted from stock aliquots in sterile saline such that the final concentration of dmso was only  <dig> % and mice were appropriately rehydrated.
the control group received an equivalent volume saline injection.
injections were performed subcutaneously immediately following surgical procedures, 6 h post-hemorrhage, and at 12-h intervals thereafter until the 72-h endpoint.
neurobehavioral testing
functional outcomes were assessed at  <dig>   <dig>  and 72 h after ich with the following neurobehavioral tests: grip strength, hanging wire task, accelerating rotarod performance, and open field locomotor activity.
for all tests, baseline function was assessed the day prior to surgery.
testing was performed during the dark cycle  by investigators blinded to treatment group.
each test was performed at the same time of day and mice were allowed 30–45 min of rest between tests.
grip strength: forelimb strength was measured using the animal grip strength system .
mice were suspended by the tail over the testing grid until their forepaws had a grip on the steel bar and then pulled away from the grid until they released their grip.
five consecutive trials per mouse were performed for each testing period.
data are reported as the average maximal force recorded prior to the mouse releasing its hold on the bar.
hanging wire task: mice were allowed to grip a steel wire suspended 50 cm above a padded flat surface with their two forelimbs and the latency to fall was recorded to evaluate muscle strength and condition.
accelerating rotarod performance: mice were assessed for motor deficits and coordination, endurance, and balance using an accelerating rotarod rotamex- <dig> machine and software .
mice were trained on the three consecutive days prior to surgery, where the last training period served as baseline functioning.
rotational speed started at 5 rpm and ended at 50 rpm and the latency to fall was automatically collected by the rotamex- <dig> software.
open field locomotor activity: ambulatory time was measured using an automated open field activity monitor and video tracking interface system .
briefly, mice were placed individually in four transparent acrylic cages and their activity was recorded over a 20-min test period.
data are represented as ambulatory counts omitting the first 5 min to exclude for initial anxiety responses.
histological procedures and quantification
at 72 h post-ich, mice were deeply anesthetized and transcardially perfused with phosphate-buffered saline  followed by 4% paraformaldehyde .
brains were collected and stored in 4% pfa for at least 24 h prior to cryopreservation in a 30% sucrose/pbs solution.
sections were processed at 30 µm using a leica cm  <dig> cryostat  and stored at −80°c for later histological procedures.
cresyl violet staining was used for determination of lesion volume  <cit> .
to assess ferric iron content, perl’s iron staining was completed by incubating slides in a 1: <dig> mix of 2% hydrochloric acid and 2% potassium ferrocyanide for 20 min, followed by counterstaining with nuclear fast red.
immunohistochemistry was performed to evaluate microglial activation, astrogliosis, leukocyte transendothelial migratory potential, neutrophil infiltration, and bbb breakdown using the following primary antibodies: ionized calcium-binding adapter protein  <dig> , 1: <dig> ; glial fibrillary acidic protein , 1: <dig> ; platelet endothelial cell adhesion molecule  <dig> , 1: <dig> ; myeloperoxidase , 1: <dig> ; and immunoglobulin g , 1: <dig> , respectively.
a secondary biotinylated antibody was used for detection , except for igg staining, which used a biotinylated primary anti-mouse igg antibody.
the vectastain elite abc kit and dab kit  were used per manufacturer’s instructions for the avidin-peroxidase step and final dab reaction, respectively.
gfap and iba <dig> slides were not counterstained, mpo and igg slides were counterstained with cresyl violet, and pecam- <dig> slides were counterstained with nuclear fast red.
after cresyl violet, perl’s iron, and immunohistochemical staining, slides were dehydrated in increasing concentrations of ethanol and coverslipped with permount.
all slides were scanned using a scanscope cs and analyzed with imagescope software .
lesion volume was determined by outlining of the injured areas on  <dig> sections equally distributed throughout the entire hematoma and anteroposterior brain regions.
lesion areas for each section were abstracted from the imagescope software and a lesion volume was calculated using these areas, known distance between each section, and section thickness.
ferric iron content and immunohistochemical stains were quantified using the imagescope ‘positive pixel count’ algorithm after the appropriate brain regions were outlined .
algorithms were tuned for each of the stains such that the appropriate signal and strength of signal was detected.
as an example, moderate and strongly positive blue pixels are detected in perl’s iron slides, and not weakly positive pixels because these could potentially represent non-specific signal.
for each stain, the same four sections representing maximal lesion areas were used.
cortical microgliosis was analyzed by placing identically sized boxes of  <dig>  by  <dig>  pixels in the ipsilateral and contralateral cortex.
data are presented as the relative ipsilateral to contralateral signal.
striatal microgliosis was analyzed by outlining of the ipsilateral and contralateral striatum, excluding the lesion area.
data are presented as the ipsilateral signal per area quantified with normalization for the contralateral signal per area quantified.
cortical and striatal astrogliosis were analyzed in a similar manner to microgliosis; however, these data were not normalized due to negligible signal in the contralateral cortex and striatum with the thresholds used here.
cortical and striatal astrogliosis data are presented as the signal per area quantified.
perl’s, mpo, and pecam- <dig> slides were analyzed by circling of the ipsilateral hemisphere.
whole brain signal was examined for igg quantification because staining extended into the contralateral side in some cases.
after all analyses, the appropriate algorithm was run and data was abstracted from the imagescope software.
statistical analyses
graphpad prism  <dig> software was used for all statistical analyses .
differences between two groups were determined by an unpaired two-tailed parametric student’s t test.
data are expressed as mean ± sem, and p <  <dig>  was considered statistically significant in all comparisons.
RESULTS
mice were treated after ich with the selective ep <dig> receptor antagonist sc- <dig> or the ep1/ep <dig> receptor agonist 17-pt-pge <dig> and various anatomical and functional outcomes were evaluated by histological staining and neurobehavioral testing, respectively.
treatment with the antagonist resulted in a 20% mortality rate, whereas no mortality was seen in the agonist or control groups.
effect of blockade or stimulation of the ep <dig> receptor on ich-induced brain injury
striatal hemorrhages were reproducible in all treatment groups .
quantification of lesion volumes showed that sc-51089-treated animals had  <dig>  ±  <dig> % larger lesions when compared to the control group , whereas no significant differences were seen for the 17-pt-pge2-treated mice.fig.
<dig> effect of blockade or stimulation of the ep <dig> receptor on ich-induced brain injury.
antagonist , agonist , or vehicle  was administered subcutaneously at the onset of injury, 6 h post-ich, and at 12-h intervals thereafter.
seventy-two hours after ich, brains were harvested and sections processed for cresyl violet staining and lesion volume determination.
a representative photomicrographs of coronal brain sections from control , sc-51089- , and 17-pt-pge2-  treated mice.
scale bar 1 mm.
b quantification showed that sc-51089-treated mice had significantly more ich-induced brain injury, whereas no significant differences were seen with 17-pt-pge <dig> treatment.
*p <  <dig>  when compared to the control group, n = 8– <dig> per group.
effect of blockade or stimulation of the ep <dig> receptor on functional outcomes
neurobehavioral testing was performed daily following ich by investigators blinded to treatment group.
all mice within the study participated in all behavioral testing and no significant differences in baseline functioning were seen between the treatment groups on any of the behavioral tests employed here.
the 17-pt-pge2-treated mice exhibited improved neurologic function when compared to the control group on two of the four behavioral tests performed, whereas no significant differences in functional recovery were seen for the sc-51089-treated mice .
grip strength: the average baseline grip strength for the control and 17-pt-pge <dig> groups were  <dig>  ±  <dig>  and  <dig>  ±  <dig>  g, respectively, and these values were not statistically different.
at all testing periods post-ich, all treatment groups demonstrated significantly impaired grip strength when compared to baseline testing .
however, at 24 h post-ich, 17-pt-pge2-treated mice had significantly improved forelimb strength when compared to the control group .
these mice continued to have better forelimb muscular function at 48 h  and 72 h  post-ich.
hanging wire task: mice treated with 17-pt-pge <dig> were able to hang for longer times on a suspended wire when compared to the control group at 24 h after ich .
average baseline latency to fall from the wire was not significantly different between the two groups .
there were no significant differences in performance on an accelerating rotarod  or open field locomotor activity  for the 17-pt-pge2-treated group.
for the sc-51089-treated mice, no significant differences in functional outcomes were seen at any time point post-ich with the behavioral tests used in this study.fig.
<dig> effect of blockade or stimulation of the ep <dig> receptor on functional outcomes after ich.
antagonist , agonist , or vehicle  was administered subcutaneously at the onset of injury, 6 h post-ich, and at 12-h intervals thereafter.
neurobehavioral testing was performed at  <dig>   <dig>  and 72 h after ich by individuals blinded to the treatment groups.
no significant differences in baseline functioning were seen between the treatment groups on any of the neurobehavioral tests.
a grip strength testing showed that mice treated with 17-pt-pge <dig> had significantly improved forelimb strength at all testing time points post-ich, whereas no significant differences were seen with sc- <dig> treatment.
b mice treated with 17-pt-pge <dig> had significantly improved latency to fall on the hanging wire task at 24 h following ich, whereas no significant differences were seen with sc- <dig> treatment.
c mice in all treatment groups displayed similar performance on an accelerating rotarod after ich.
d treatment with sc- <dig> or 17-pt-pge <dig> did not affect post-ich ambulatory activity.
*p <  <dig>  and **p <  <dig>  when compared to the control group, n = 8– <dig> per group.
effect of blockade or stimulation of the ep <dig> receptor on brain ferric iron content
to begin analyzing the mechanisms involved in ep1-mediated neuroprotection following ich, perl’s iron staining was performed to assess brain ferric iron content.
ferric iron predominately accumulated in perihematomal regions in all treatment groups .
quantification revealed that sc-51089-treated animals had  <dig>  ±  <dig> % more ferric iron in the ipsilateral hemisphere when compared to the control group , whereas no significant differences were seen for the 17-pt-pge2-treated mice.
no ferric iron deposition was seen in the contralateral hemisphere for any of the mice in the study.fig.
<dig> brain ferric iron content following ich and blockade or stimulation of the ep <dig> receptor.
antagonist , agonist , or vehicle  was administered subcutaneously at the onset of injury, 6 h post-ich, and at 12-h intervals thereafter.
seventy-two hours after ich, brains were harvested and sections processed for perl’s staining and ferric iron content determination.
a representative high magnification photomicrographs showing ferric iron  accumulation in the perihematomal regions of control , sc-51089- , and 17-pt-pge2-  treated mice.
square selections in the insets denote magnified regions.
scale bars on the magnified images and insets are 100 µm and 1 mm, respectively.
b quantification of brain ferric iron content in the ipsilateral hemisphere showed that sc-51089-treated mice had significantly more ferric iron accumulation, whereas no significant differences were seen for the 17-pt-pge2-treated mice.
no ferric iron deposition was seen in the contralateral hemisphere for any of the mice in the study.
*p <  <dig>  when compared to the control group, n = 8– <dig> per group.
effect of blockade or stimulation of the ep <dig> receptor on microglial activation and astrogliosis
to further delineate mechanisms, cortical and striatal microglial activation and astrogliosis were identified by iba <dig> and gfap immunohistochemistry, respectively.
following ich, sc-51089-treated mice displayed more cortical microglial activation and morphological changes when compared to the control group, whereas no significant differences were seen in striatal microgliosis or for the 17-pt-pge2-treated mice.
all treatment groups displayed increased cortical  and striatal  microglial activation and morphological changes in the ipsilateral hemisphere relative to the contralateral equivalent areas.
quantification of cortical iba <dig> immunoreactivity showed that sc-51089-treated mice had  <dig>  ±  <dig> % more microgliosis when compared to the control group .
cortical microgliosis was not significantly different for the 17-pt-pge2-treated group .
no significant differences in striatal microgliosis were seen for either treatment group .fig.
<dig> iba <dig> expression following ich and blockade or stimulation of the ep <dig> receptor.
antagonist , agonist , or vehicle  was administered subcutaneously at the onset of injury, 6 h post-ich, and at 12-h intervals thereafter.
seventy-two hours after ich, brains were harvested and sections processed for iba <dig> immunohistochemistry to evaluate cortical and striatal microgliosis.
a, b representative high magnification photomicrographs showing the ipsilateral and contralateral a cortex and b striatum for iba <dig> immunohistochemistry of coronal brain sections from control , sc-51089- , and 17-pt-pge2-  treated mice.
square selections in the insets denote magnified regions.
scale bars on the magnified images and insets are 100 µm and 2 mm, respectively.
c, d quantification of iba <dig> immunoreactivity demonstrated that sc-51089-treated mice had significantly more c cortical microgliosis, whereas no significant differences were seen in  striatal microgliosis.
this increased cortical iba <dig> immunoreactivity was accompanied by more cellular morphological changes.
no significant differences in c cortical or d striatal microgliosis were seen for the 17-pt-pge2-treated mice.
data is normalized to the corresponding contralateral equivalent signal with appropriate control for the area of quantification in striatal analyses .
*p <  <dig>  when compared to the control group, n = 8– <dig> per group.
when compared to the control group, 17-pt-pge2-treated mice had significantly less cortical and striatal astrogliosis, whereas no significant differences were seen with sc- <dig> treatment.
similar to microgliosis, all treatment groups displayed significantly increased ipsilateral cortical  and striatal  astrogliosis compared to the contralateral equivalent areas.
quantification of gfap immunoreactivity showed that 17-pt-pge2-treated mice had  <dig>  ±  <dig> % less cortical astrogliosis  and  <dig>  ±  <dig> % less striatal astrogliosis .
no significant differences in cortical  or striatal  astrogliosis were seen for the sc-51089-treated group.fig.
<dig> gfap expression following ich and blockade or stimulation of the ep <dig> receptor.
antagonist , agonist , or no treatment  was administered subcutaneously at the onset of injury, 6 h post-ich, and at 12-h intervals thereafter.
seventy-two hours after ich, brains were harvested and sections processed for gfap immunohistochemistry to evaluate cortical and striatal astrogliosis.
a, b representative high magnification photomicrographs showing the ipsilateral and contralateral a cortex and b striatum for gfap immunohistochemistry of coronal brain sections from control , sc-51089- , and 17-pt-pge2-  treated mice.
square selections in the insets denote magnified regions.
scale bars on the magnified images and inserts are 100 µm and 2 mm, respectively.
c, d quantification of gfap immunoreactivity demonstrated that 17-pt-pge2-treated mice had significantly less c cortical and d striatal astrogliosis, whereas no significant differences were seen for sc-51089-treated mice.
all treatment groups demonstrated negligible staining in the contralateral cortex and striatum; thus, data is presented as the ipsilateral gfap immunoreactivity corrected for the area of quantification, without normalization for the contralateral equivalent areas.
**p <  <dig>  and ***p <  <dig>  when compared to the control group, n = 8– <dig> per group.
effect of blockade or stimulation of the ep <dig> receptor on leukocyte transendothelial migratory potential, peripheral neutrophil infiltration, and bbb breakdown
additional immunohistochemical staining for pecam- <dig>  mpo, and igg was performed to identify leukocyte transendothelial migratory potential, peripheral neutrophil infiltration, and bbb breakdown, respectively.
pecam- <dig>  and mpo  staining was only observed within injured brain regions.
igg staining was diffusely present throughout the ipsilateral hemisphere and crossed into the contralateral hemisphere in some cases .
quantification showed that 17-pt-pge2-treated mice had  <dig>  ±  <dig> % less pecam- <dig> immunoreactivity when compared to the control group .
likewise, these mice also had  <dig>  ±  <dig> % less neutrophil infiltration  and  <dig>  ±  <dig> % less igg immunoreactivity .
no significant differences in pecam- <dig>  mpo, or igg immunoreactivity were observed for the sc-51089-treated group.fig.
<dig> pecam- <dig>  mpo, and igg immunoreactivity following ich and blockade or stimulation of the ep <dig> receptor.
antagonist , agonist , or vehicle  was administered subcutaneously at the onset of injury, 6 h post-ich, and at 12-h intervals thereafter.
seventy-two hours after ich, brains were harvested and sections processed for pecam- <dig>  mpo, and igg immunohistochemistry to evaluate leukocyte transendothelial migratory potential, neutrophil infiltration, and bbb breakdown, respectively.
a, b, c representative high magnification photomicrographs showing a pecam- <dig> staining highlighting blood vessels within the lesion, b mpo-positive neutrophils within the lesion, and c igg staining within the lesion and surrounding areas of coronal brain sections from control , sc-51089- , and 17-pt-pge2-  treated mice.
square selections in the insets denote magnified regions.
scale bars on the magnified images and inserts are 100 µm and 2 mm, respectively.
d, e, f quantification revealed that 17-pt-pge2-treated mice had significantly less d pecam- <dig> staining, e neutrophil infiltration, and f bbb breakdown.
all quantification data are presented at the same scale such that the relative d pecam- <dig>  e mpo, and f igg immunoreactivity can be directly compared.
no significant differences were seen for the sc-51089-treated mice.
*p <  <dig>  and **p <  <dig>  when compared to the control group, n = 7– <dig> per group.
discussion
we have shown that chronic post-treatment with sc- <dig>  a selective ep <dig> receptor antagonist, aggravates brain injury after ich, resulting in larger lesion volumes and more iron accumulation, whereas treatment with 17-pt-pge <dig>  an ep <dig> receptor agonist with extensive ep <dig> receptor agonism, results in no detectable changes in lesion volume or iron deposition, but improves neuromuscular functional recovery.
the two treatments resulted in differential patterns of microglial and astrocyte activation, with ep <dig> antagonism producing increased cortical microgliosis and ep1/ep <dig> agonism  resulting in reduced cortical and striatal astrogliosis.
last, treatment with the ep <dig> agonist reduced leukocyte transendothelial migratory potential, neutrophil infiltration, and bbb breakdown.
our results demonstrating that post-ich ep <dig> antagonism exacerbates brain injury are in agreement with our previous study that showed significantly more ich-induced brain injury and worse functional outcomes in mice with genetic deletion of the ep <dig> receptor  <cit> , although we did not observe smaller lesion volumes with the ep <dig> agonist used in this study, 17-pt-pge <dig>  this ep <dig> agonist has ki values of  <dig>  and  <dig>  nm for the ep <dig> and ep <dig> receptors  <cit> , respectively, and the non-selective nature could have led to the outcomes seen here.
in support of this hypothesis, we have recently shown that ep3−/− mice have less ich-induced brain injury when compared to wt controls, implying an injurious role for pge2-ep <dig> signaling that is opposite to the neuroprotective effects of the ep <dig> receptor  <cit> .
therefore, it is likely that the overall desired beneficial effect of 17-pt-pge <dig> by agonism of the ep <dig> receptor is negated by simultaneous ep <dig> receptor agonism, resulting in no observed differences with 17-pt-pge <dig> treatment when compared to the control group.
it is also possible that the dose used here for agonist treatment  may not be high enough to affect lesion volume after ich.
interestingly, the 17-pt-pge2-treated mice performed better on neurobehavioral testing, including grip strength examination and the hanging wire task.
in our aforementioned observations with ep3−/− mice, we did not find the same extent of differences on neurobehavioral testing as compared to ep1−/− mice, and thus collectively these data imply an important role for the pge2-ep <dig> signaling axis in post-ich functional recovery.
additional studies with 17-pt-pge <dig> treatment in the appropriate ep receptor knockout animals would be necessary to verify this suggestion.
furthermore, studies in other ich models and using additional selective ep <dig> receptor agonists, with a dose-response curve and multiple endpoints, will clarify the therapeutic potential of targeting the ep <dig> receptor for the treatment of ich.
nevertheless, the main finding that post-treatment with a selective ep <dig> antagonist aggravates ich-induced brain injury supports our previous findings suggesting an important role for the ep <dig> receptor in positively modulating ich outcomes.
many studies have shown differential effects of modulating pge2-ep <dig> receptor signaling on outcomes in models of acute and chronic neurologic disorders  <cit> , including contradictory results in the various stroke models.
it appears that ep <dig> receptor agonism and antagonism may be useful under hemorrhagic and ischemic conditions, respectively  <cit> .
hemolysis following ich is an important trigger in the progression of brain injury.
the liberated free heme generates ferric ions , which via the fenton reaction produce reactive oxygen species, inducing oxidative stress.
to understand whether ep <dig> receptor activation or inhibition modulates ferric iron content, brain sections were subjected to perl’s iron staining.
analogous to the effects seen with sc- <dig> and 17-pt-pge <dig> treatment on lesion volume, we found an increase in the ferric iron content of antagonist-treated mice, while no significant differences were observed in the agonist-treated group.
these data suggest that inhibition of pge2-ep <dig> receptor signaling prevents the clearance of iron from injured brain regions, which may be a consequence of impaired microglial phagocytosis with ep <dig> blockade since deletion of this receptor has been reported to reduce phagocytic capability  <cit> .
however, another possible explanation is that antagonist-treated mice have more bleeding, leading to more accumulation of iron in the hemorrhagic area.
the latter hypothesis is supported by our previous findings indicating that deletion of the ep <dig> receptor increases cerebral blood flow  <cit> .
microglial and astrocyte activation is another criterion to gauge injury severity and monitor the neuroinflammatory response.
following ich, microglia likely play a dual role where they are protective in their cleanup of red blood cells and damaged brain tissue, thereby promoting repair, but toxic in their facilitation of neuroinflammation and recruitment of leukocytes through the production of proinflammatory cytokines  <cit> .
here, significantly increased microgliosis was seen in ipsilateral cortical regions with ep <dig> antagonist treatment, whereas no differences were seen in the ipsilateral striatal regions of the same group or in the agonist-treated group.
we have previously shown that the ep <dig> receptor colocalizes with microglial markers in perihematomal regions and that receptor deletion reduces the activated microglial population and phagocytic function  <cit> .
several possibilities exist to explain these contradictory striatal microgliosis findings in ep1−/− mice and wt mice treated with an ep <dig> antagonist:  prostaglandin receptors are reported to cross talk  <cit> , and it is possible that ep <dig> deletion caused the expression of another prostanoid receptor important for regulating microglial activation state to change,  deletion of the ep <dig> receptor reduces basal levels of microglial activation, and  ep1−/− mice may develop some other compensatory mechanism.
in comparing cortical and striatal post-ich microglial activation state with ep <dig> antagonism, cortical microglia appear to retain their ability to activate and respond to the injury, indicating a brain region-specific response.
astrocytes play diverse roles in response to brain injury, where they try to contain the damage be forming a glial scar  <cit> .
however, excessive or sustained astrocyte activation can be detrimental and augment brain damage and functional deficits by contributing to chronic inflammation and cell death.
treatment with the antagonist had no effect on astrogliosis, whereas the agonist significantly reduced astrogliosis in the ipsilateral cortical and striatal regions.
strong evidence exists suggesting that impairment of astrogliosis improves axonal growth and improves functional outcomes following brain injury  <cit> .
here, we observed a significant decrease in cortical and striatal astrogliosis in the agonist-treated group, whereas no significant differences were seen with ep <dig> antagonist treatment.
intriguingly, this reduced astrogliosis with 17-pt-pge <dig> treatment was accompanied by better neuromuscular functional recovery.
the discrepancy in astrocyte and microglial activation with ep <dig> antagonism or agonism  may also result from the unbalanced influence of pge2-ep <dig> receptor signaling between the treatment groups.
finally, we evaluated leukocyte transendothelial migratory potential, leukocyte infiltration, and bbb disruption, where we found less immunoreactivity for markers of these outcome measures in the agonist-treated group and no significant differences with ep <dig> antagonism.
as previously mentioned in the context of lesion volume, neurobehavioral testing, and gliosis, the interpretation of these data is complicated given the lack of opposing results between the two treatment groups and non-selective nature of the agonist toward ep1/ep <dig> receptors; although, it does suggest that the ep <dig> receptor may play a larger role in modulating these particular outcomes when compared to the contribution of pge2-ep <dig> signaling.
ich initially directly disrupts the bbb, leading to a massive neuroinflammatory cascade that is characterized by invasion of the lesion cite by leukocytes and further damage to the vasculature.
there are various triggers that can lead to secondary bbb dysfunction after ich.
importantly, blood products  and the inflammatory response, including that mediated by endogenous and infiltrating cells, play a critical role in ich-induced bbb dysfunction  <cit> .
as previously mentioned, we found that treatment with the ep <dig> antagonist increased brain ferric iron content, whereas the ep <dig> agonist had no effect.
higher iron load after ich means a greater potential for oxidative damage and inflammation, ultimately leading to more bbb damage.
however, we did not observe an increase in bbb dysfunction in the antagonist-treated group; instead, we observed improved bbb integrity  in the agonist-treated mice that did not display less ferric iron content or smaller lesion volumes .
these findings suggest that in the present study, cell-mediated inflammatory responses may be primarily responsible for the observed differences in bbb integrity between the treatment groups.
endogenous cell types such as microglia and astrocytes can secrete factors that modulate bbb permeability, especially with excessive or sustained activation.
here, we have shown that agonist-treated mice display significantly reduced activation of one of the key cell types responsible for maintenance of the bbb, astrocytes, and this reduced astrogliosis could be contributing to the overall preservation of bbb integrity with 17-pt-pge <dig> treatment.
however, infiltrating leukocytes such as neutrophils migrate to the site of injury and augment the neuroinflammatory cascade, causing further damage to the bbb.
our findings show that treatment with 17-pt-pge <dig> decreases neutrophil infiltration, as monitored by immunohistochemical staining for mpo.
in the current experimental setting, it is not possible to distinguish the respective contribution of astrogliosis and leukocyte infiltration in modulating bbb integrity, although both processes seem to play a role.
leukocytes cross the endothelium using paracellular and transcellular pathways .
pecam- <dig> is expressed on the surface of several types of leukocytes  and lateral borders of endothelial cells, and is involved in the transmigration phase of leukocyte emigration through homophilic interactions  <cit> .
neutrophil transmigration capability, as tested by pecam- <dig> staining, was found to be lower in mice treated with 17-pt-pge <dig>  implying that ep1/ep <dig> agonism preserves the bbb barrier and reduces leukocyte infiltration by sealing intercellular gaps between endothelial cells, and probably more importantly, decreasing the engagement of migrating leukocytes.
CONCLUSIONS
in this study we have provided additional evidence consistent with our previous findings suggesting a neuroprotective role for the ep <dig> receptor following ich.
our results show that blockade of the pge2-ep <dig> signaling axis aggravates ich-induced brain injury and increases ferric iron deposition and microgliosis, whereas stimulation decreases astrogliosis, leukocyte transendothelial migratory potential, neutrophil infiltration, and bbb breakdown and improves neuromuscular functional recovery.
caution should be used when interpreting and extrapolating these results because it is likely that a portion of the findings are due to activation of ep <dig> signaling rather than ep <dig>  given the non-selective nature of the agonist used in this study.
even so, ep1-mediated post-ich neuroprotection is probably achieved by positive modulation of microglial phagocytosis and/or decreased bleeding tendency, leading to lower iron overload, less oxidative processes, and attenuated neuroinflammatory responses.
additional studies with other ich models, a selective ep <dig> agonist, and pharmacologic manipulation of the ep <dig> receptor will clarify the respective role of these pge2-mediated signaling axes after ich.
nonetheless, the ep <dig> receptor remains a viable therapeutic target for the treatment of ich.
abbreviations
abcavidin/biotin enzyme complex
adalzheimer’s disease
bbbblood brain barrier
campcyclic adenosine monophosphate
coxcyclooxygenase
dab <dig> ′-diaminobenzidine
dmsodimethyl sulfoxide
ep1e prostanoid receptor subtype 1
ep3e prostanoid receptor subtype 3
ep1-/−ep <dig> receptor knockout
gfapglial fibrillary acidic protein
hiehypoxic-ischemic encephalopathy
iba1ionized calcium-binding adapter protein 1
ichintracerebral hemorrhage
iggimmunoglobulin g
mpgesmembrane-bound pge synthase
mpomyeloperoxidase
pbsphosphate-buffered saline
pdparkinson’s disease
pecam-1platelet endothelial cell adhesion molecule 1
pfaparaformaldehyde
pge2prostaglandin e2
semstandard error of the mean
tbitraumatic brain injury
wtwildtype
jenna l leclerc and abdullah s ahmad contributed equally to this work
authors’ contributions
jll, ass, and sd designed the study, interpreted results, and wrote the manuscript.
jll coordinated histology and blinded quantification, and analyzed data; ns performed surgical procedures, coordinated blinded behavioral testing, and assisted with histology; ls performed behavioral analyses; ls, eg, and ad contributed to histological staining, quantification, and data analysis.
all authors read and approved the final manuscript.

 intracerebral hemorrhage is the most debilitating and is associated with the highest mortality rates of all stroke subtypes, has no effective therapeutics, and represents a major public health problem. ich is a multifaceted disorder with acute and chronic mechanisms of injury, including initial mechanical damage due to the expanding hematoma and compression of brain tissue and later inflammatory/oxidative processes primarily resulting from the presence of blood components. combating the strong neuroinflammatory cascade after ich remains a promising therapeutic target to reduce secondary brain damage and improve patient outcomes. neuroinflammation following brain injury is intimately connected to upregulation of cyclooxygenase enzymes, mainly the inducible isoform cox- coordinated expression of membrane-bound pge synthase-, and consequent increased production of prostaglandins. prostaglandins have been shown to modulate outcomes in a variety of neurological disorders, including stroke, traumatic brain injury, epilepsy, alzheimer’s disease, and parkinson’s disease, among others. although selective inhibition of cox- has been reported to improve outcomes in many preclinical models of these neurological disorders, including ich, the clinical use of these drugs is limited due to the potential for known severe side effects. therefore, targeting more specific downstream pathways of cox/mpges-mediated prostaglandin signaling may reduce these deleterious side effects while still harnessing the already characterized positive immunomodulatory properties seen with cox- inhibition. prostaglandin e is a major product of cox/pges activity in the central nervous system and is classically thought of as proinflammatory within the brain. however, emerging evidence suggests a complex scenario with pge capable of exerting pro- and/or anti-inflammatory functions depending on the underlying neuropathophysiology. the many actions of pge are produced by its differential stimulation of mainly the g-protein-coupled e prostanoid receptors 1- which display varied anatomical distributions, cellular expression profiles, ligand binding affinity, desensitization kinetics, and signal transduction pathways. in the present study, we focus on the ep receptor, which is reported to be expressed in the thalamus/hypothalamus, hippocampus, cortex, striatum, and cerebellum. ep stimulation results in increased intracellular calcium levels via coupling to a gαq protein, phosphatidyl inositol hydrolysis, and extracellular calcium influx. we and others have documented the pro- and/or anti-inflammatory effects and neurotoxic or neuroprotective properties of signaling through the pge2-ep axis in various models of acute and chronic neurological disorders such as stroke, hypoxic-ischemic encephalopathy, traumatic brain injury, ad, and pd. in models of transient forebrain ischemia, focal ischemia, and excitotoxicity, pge2-ep signaling aggravates brain damage possibly by increasing blood brain barrier disruption and vascular tone and decreasing neuronal resistance to oxidative stress and cell death. similarly, the neurotoxicity associated with pge2-ep signaling was shown in a combined model of ad and cerebral ischemia, where amyloid plaques and neuronal damage were reduced in ep1-deleted ad mice following ischemic insult. further, inhibition of ep with a selective antagonist reduced hie cerebral injury. in a model of toxin-induced parkinsonism, dopaminergic neurons were protected and apomorphine-induced contralateral rotations were decreased in ep1−/− mice. on the contrary, deletion or antagonism of the ep receptor failed to provide neuroprotection in a contusive model of tbi. last, we have recently shown that genetic deletion of the ep receptor aggravates ich-induced brain injury and impairs functional recovery, where mechanistic evidence was provided to suggest ep stimulation positively modulates microglial activation and phagocytosis. thus, signaling through the pge2-ep axis has a complex role following brain injury and appears to depend on the underlying pathologic processes, and possibly also on the genetic and/or pharmacologic approach used. the present study aimed to explore our prior data in ep1−/− mice by extension to post-ich pharmacologic manipulation of the ep receptor. in line with our previous results, here we show that selective antagonism of the ep receptor with sc- chronically following ich exacerbates brain injury and increases ferric iron deposition and microgliosis. furthermore, repeated administration of 17-pt-pge an ep agonist, after ich improved functional recovery and reduced astrogliosis, leukocyte transendothelial migratory potential, peripheral neutrophil infiltration, and bbb breakdown. methods mice experiments were performed with 11–13-week-old c57bl/ male mice. the colony was bred and maintained in our animal facilities in a temperature-controlled reverse light cycle environment so that behavioral testing could be performed during the awaken phase. mice were allowed free access to food and water before and after surgical procedures. all animal protocols were approved by the university of florida institutional animal care and use committee. ich model ich was induced by unilateral intrastriatial infusion of collagenase type vii-s as we have described previously. briefly, mice were anesthetized with isoflurane and immobilized on a stereotactic frame. after making a small incision in the skin overlying the skull, a craniotomy was performed  mm anterior and  mm right of bregma. collagenase was infused  mm ventral from the dura over a 5-min period using a syringe with a 26-gauge blunt tip needle. rectal temperatures were maintained at  ±  °c throughout all surgical procedures and mice were allowed to recover in temperature- and humidity-controlled chambers for at least 1 h postoperatively. experimental groups and drug treatments mice were equally distributed into three treatment groups: antagonist, agonist, and control. the antagonist 8-chloro-2-hydrazide-dibenz oxazepine-10-carboxylic acid, monohydrochloride] is selective for the ep receptor and was administered at a dose of 10 µg/kg of body weight. intraperitoneal treatment with this dose either 5 min or 6 h after reperfusion significantly reduced infarct volume and brain swelling and improved neurological deficits in the transient middle cerebral artery occlusion stroke model. the agonist 9-oxo-11α,15s-dihydroxy-17-phenyl-,20-trinor-prosta-5z,13e-dien-1-oic acid is non-selective for the ep receptor, with ki values of and  nm for the ep and ep receptors, respectively. the dose of  mg/kg of body weight was chosen based on a previous study where intravenous pretreatment was shown to aggravate histamine-mediated gastric mucosal injury. sc- and 17-pt-pge were purchased from cayman chemicals. stock solutions were prepared in dimethyl sulfoxide and subsequently aliquoted and stored at −20°c. solutions for injections were freshly diluted from stock aliquots in sterile saline such that the final concentration of dmso was only % and mice were appropriately rehydrated. the control group received an equivalent volume saline injection. injections were performed subcutaneously immediately following surgical procedures, 6 h post-hemorrhage, and at 12-h intervals thereafter until the 72-h endpoint. neurobehavioral testing functional outcomes were assessed at and 72 h after ich with the following neurobehavioral tests: grip strength, hanging wire task, accelerating rotarod performance, and open field locomotor activity. for all tests, baseline function was assessed the day prior to surgery. testing was performed during the dark cycle by investigators blinded to treatment group. each test was performed at the same time of day and mice were allowed 30–45 min of rest between tests. grip strength: forelimb strength was measured using the animal grip strength system. mice were suspended by the tail over the testing grid until their forepaws had a grip on the steel bar and then pulled away from the grid until they released their grip. five consecutive trials per mouse were performed for each testing period. data are reported as the average maximal force recorded prior to the mouse releasing its hold on the bar. hanging wire task: mice were allowed to grip a steel wire suspended 50 cm above a padded flat surface with their two forelimbs and the latency to fall was recorded to evaluate muscle strength and condition. accelerating rotarod performance: mice were assessed for motor deficits and coordination, endurance, and balance using an accelerating rotarod rotamex- machine and software. mice were trained on the three consecutive days prior to surgery, where the last training period served as baseline functioning. rotational speed started at 5 rpm and ended at 50 rpm and the latency to fall was automatically collected by the rotamex- software. open field locomotor activity: ambulatory time was measured using an automated open field activity monitor and video tracking interface system. briefly, mice were placed individually in four transparent acrylic cages and their activity was recorded over a 20-min test period. data are represented as ambulatory counts omitting the first 5 min to exclude for initial anxiety responses. histological procedures and quantification at 72 h post-ich, mice were deeply anesthetized and transcardially perfused with phosphate-buffered saline followed by 4% paraformaldehyde. brains were collected and stored in 4% pfa for at least 24 h prior to cryopreservation in a 30% sucrose/pbs solution. sections were processed at 30 µm using a leica cm cryostat and stored at −80°c for later histological procedures. cresyl violet staining was used for determination of lesion volume. to assess ferric iron content, perl’s iron staining was completed by incubating slides in a 1: mix of 2% hydrochloric acid and 2% potassium ferrocyanide for 20 min, followed by counterstaining with nuclear fast red. immunohistochemistry was performed to evaluate microglial activation, astrogliosis, leukocyte transendothelial migratory potential, neutrophil infiltration, and bbb breakdown using the following primary antibodies: ionized calcium-binding adapter protein, 1:; glial fibrillary acidic protein, 1:; platelet endothelial cell adhesion molecule, 1:; myeloperoxidase, 1:; and immunoglobulin g, 1:, respectively. a secondary biotinylated antibody was used for detection, except for igg staining, which used a biotinylated primary anti-mouse igg antibody. the vectastain elite abc kit and dab kit were used per manufacturer’s instructions for the avidin-peroxidase step and final dab reaction, respectively. gfap and iba slides were not counterstained, mpo and igg slides were counterstained with cresyl violet, and pecam- slides were counterstained with nuclear fast red. after cresyl violet, perl’s iron, and immunohistochemical staining, slides were dehydrated in increasing concentrations of ethanol and coverslipped with permount. all slides were scanned using a scanscope cs and analyzed with imagescope software. lesion volume was determined by outlining of the injured areas on sections equally distributed throughout the entire hematoma and anteroposterior brain regions. lesion areas for each section were abstracted from the imagescope software and a lesion volume was calculated using these areas, known distance between each section, and section thickness. ferric iron content and immunohistochemical stains were quantified using the imagescope ‘positive pixel count’ algorithm after the appropriate brain regions were outlined. algorithms were tuned for each of the stains such that the appropriate signal and strength of signal was detected. as an example, moderate and strongly positive blue pixels are detected in perl’s iron slides, and not weakly positive pixels because these could potentially represent non-specific signal. for each stain, the same four sections representing maximal lesion areas were used. cortical microgliosis was analyzed by placing identically sized boxes of by pixels in the ipsilateral and contralateral cortex. data are presented as the relative ipsilateral to contralateral signal. striatal microgliosis was analyzed by outlining of the ipsilateral and contralateral striatum, excluding the lesion area. data are presented as the ipsilateral signal per area quantified with normalization for the contralateral signal per area quantified. cortical and striatal astrogliosis were analyzed in a similar manner to microgliosis; however, these data were not normalized due to negligible signal in the contralateral cortex and striatum with the thresholds used here. cortical and striatal astrogliosis data are presented as the signal per area quantified. perl’s, mpo, and pecam- slides were analyzed by circling of the ipsilateral hemisphere. whole brain signal was examined for igg quantification because staining extended into the contralateral side in some cases. after all analyses, the appropriate algorithm was run and data was abstracted from the imagescope software. statistical analyses graphpad prism software was used for all statistical analyses. differences between two groups were determined by an unpaired two-tailed parametric student’s t test. data are expressed as mean ± sem, and p <  was considered statistically significant in all comparisons.  mice were treated after ich with the selective ep receptor antagonist sc- or the ep1/ep receptor agonist 17-pt-pge and various anatomical and functional outcomes were evaluated by histological staining and neurobehavioral testing, respectively. treatment with the antagonist resulted in a 20% mortality rate, whereas no mortality was seen in the agonist or control groups. effect of blockade or stimulation of the ep receptor on ich-induced brain injury striatal hemorrhages were reproducible in all treatment groups. quantification of lesion volumes showed that sc-51089-treated animals had  ±  % larger lesions when compared to the control group, whereas no significant differences were seen for the 17-pt-pge2-treated mice.fig.  effect of blockade or stimulation of the ep receptor on ich-induced brain injury. antagonist, agonist, or vehicle was administered subcutaneously at the onset of injury, 6 h post-ich, and at 12-h intervals thereafter. seventy-two hours after ich, brains were harvested and sections processed for cresyl violet staining and lesion volume determination. a representative photomicrographs of coronal brain sections from control, sc-51089-, and 17-pt-pge2- treated mice. scale bar 1 mm. b quantification showed that sc-51089-treated mice had significantly more ich-induced brain injury, whereas no significant differences were seen with 17-pt-pge treatment. *p <  when compared to the control group, n = 8– per group. effect of blockade or stimulation of the ep receptor on functional outcomes neurobehavioral testing was performed daily following ich by investigators blinded to treatment group. all mice within the study participated in all behavioral testing and no significant differences in baseline functioning were seen between the treatment groups on any of the behavioral tests employed here. the 17-pt-pge2-treated mice exhibited improved neurologic function when compared to the control group on two of the four behavioral tests performed, whereas no significant differences in functional recovery were seen for the sc-51089-treated mice. grip strength: the average baseline grip strength for the control and 17-pt-pge groups were  ±  and  ±   g, respectively, and these values were not statistically different. at all testing periods post-ich, all treatment groups demonstrated significantly impaired grip strength when compared to baseline testing. however, at 24 h post-ich, 17-pt-pge2-treated mice had significantly improved forelimb strength when compared to the control group. these mice continued to have better forelimb muscular function at 48 h and 72 h post-ich. hanging wire task: mice treated with 17-pt-pge were able to hang for longer times on a suspended wire when compared to the control group at 24 h after ich. average baseline latency to fall from the wire was not significantly different between the two groups. there were no significant differences in performance on an accelerating rotarod or open field locomotor activity for the 17-pt-pge2-treated group. for the sc-51089-treated mice, no significant differences in functional outcomes were seen at any time point post-ich with the behavioral tests used in this study.fig.  effect of blockade or stimulation of the ep receptor on functional outcomes after ich. antagonist, agonist, or vehicle was administered subcutaneously at the onset of injury, 6 h post-ich, and at 12-h intervals thereafter. neurobehavioral testing was performed at and 72 h after ich by individuals blinded to the treatment groups. no significant differences in baseline functioning were seen between the treatment groups on any of the neurobehavioral tests. a grip strength testing showed that mice treated with 17-pt-pge had significantly improved forelimb strength at all testing time points post-ich, whereas no significant differences were seen with sc- treatment. b mice treated with 17-pt-pge had significantly improved latency to fall on the hanging wire task at 24 h following ich, whereas no significant differences were seen with sc- treatment. c mice in all treatment groups displayed similar performance on an accelerating rotarod after ich. d treatment with sc- or 17-pt-pge did not affect post-ich ambulatory activity. *p <  and **p <  when compared to the control group, n = 8– per group. effect of blockade or stimulation of the ep receptor on brain ferric iron content to begin analyzing the mechanisms involved in ep1-mediated neuroprotection following ich, perl’s iron staining was performed to assess brain ferric iron content. ferric iron predominately accumulated in perihematomal regions in all treatment groups. quantification revealed that sc-51089-treated animals had  ±  % more ferric iron in the ipsilateral hemisphere when compared to the control group, whereas no significant differences were seen for the 17-pt-pge2-treated mice. no ferric iron deposition was seen in the contralateral hemisphere for any of the mice in the study.fig.  brain ferric iron content following ich and blockade or stimulation of the ep receptor. antagonist, agonist, or vehicle was administered subcutaneously at the onset of injury, 6 h post-ich, and at 12-h intervals thereafter. seventy-two hours after ich, brains were harvested and sections processed for perl’s staining and ferric iron content determination. a representative high magnification photomicrographs showing ferric iron accumulation in the perihematomal regions of control, sc-51089-, and 17-pt-pge2- treated mice. square selections in the insets denote magnified regions. scale bars on the magnified images and insets are 100 µm and 1 mm, respectively. b quantification of brain ferric iron content in the ipsilateral hemisphere showed that sc-51089-treated mice had significantly more ferric iron accumulation, whereas no significant differences were seen for the 17-pt-pge2-treated mice. no ferric iron deposition was seen in the contralateral hemisphere for any of the mice in the study. *p <  when compared to the control group, n = 8– per group. effect of blockade or stimulation of the ep receptor on microglial activation and astrogliosis to further delineate mechanisms, cortical and striatal microglial activation and astrogliosis were identified by iba and gfap immunohistochemistry, respectively. following ich, sc-51089-treated mice displayed more cortical microglial activation and morphological changes when compared to the control group, whereas no significant differences were seen in striatal microgliosis or for the 17-pt-pge2-treated mice. all treatment groups displayed increased cortical and striatal microglial activation and morphological changes in the ipsilateral hemisphere relative to the contralateral equivalent areas. quantification of cortical iba immunoreactivity showed that sc-51089-treated mice had  ±  % more microgliosis when compared to the control group. cortical microgliosis was not significantly different for the 17-pt-pge2-treated group. no significant differences in striatal microgliosis were seen for either treatment group.fig.  iba expression following ich and blockade or stimulation of the ep receptor. antagonist, agonist, or vehicle was administered subcutaneously at the onset of injury, 6 h post-ich, and at 12-h intervals thereafter. seventy-two hours after ich, brains were harvested and sections processed for iba immunohistochemistry to evaluate cortical and striatal microgliosis. a, b representative high magnification photomicrographs showing the ipsilateral and contralateral a cortex and b striatum for iba immunohistochemistry of coronal brain sections from control, sc-51089-, and 17-pt-pge2- treated mice. square selections in the insets denote magnified regions. scale bars on the magnified images and insets are 100 µm and 2 mm, respectively. c, d quantification of iba immunoreactivity demonstrated that sc-51089-treated mice had significantly more c cortical microgliosis, whereas no significant differences were seen in striatal microgliosis. this increased cortical iba immunoreactivity was accompanied by more cellular morphological changes. no significant differences in c cortical or d striatal microgliosis were seen for the 17-pt-pge2-treated mice. data is normalized to the corresponding contralateral equivalent signal with appropriate control for the area of quantification in striatal analyses. *p <  when compared to the control group, n = 8– per group. when compared to the control group, 17-pt-pge2-treated mice had significantly less cortical and striatal astrogliosis, whereas no significant differences were seen with sc- treatment. similar to microgliosis, all treatment groups displayed significantly increased ipsilateral cortical and striatal astrogliosis compared to the contralateral equivalent areas. quantification of gfap immunoreactivity showed that 17-pt-pge2-treated mice had  ±  % less cortical astrogliosis and  ±  % less striatal astrogliosis. no significant differences in cortical or striatal astrogliosis were seen for the sc-51089-treated group.fig.  gfap expression following ich and blockade or stimulation of the ep receptor. antagonist, agonist, or no treatment was administered subcutaneously at the onset of injury, 6 h post-ich, and at 12-h intervals thereafter. seventy-two hours after ich, brains were harvested and sections processed for gfap immunohistochemistry to evaluate cortical and striatal astrogliosis. a, b representative high magnification photomicrographs showing the ipsilateral and contralateral a cortex and b striatum for gfap immunohistochemistry of coronal brain sections from control, sc-51089-, and 17-pt-pge2- treated mice. square selections in the insets denote magnified regions. scale bars on the magnified images and inserts are 100 µm and 2 mm, respectively. c, d quantification of gfap immunoreactivity demonstrated that 17-pt-pge2-treated mice had significantly less c cortical and d striatal astrogliosis, whereas no significant differences were seen for sc-51089-treated mice. all treatment groups demonstrated negligible staining in the contralateral cortex and striatum; thus, data is presented as the ipsilateral gfap immunoreactivity corrected for the area of quantification, without normalization for the contralateral equivalent areas. **p <  and ***p <  when compared to the control group, n = 8– per group. effect of blockade or stimulation of the ep receptor on leukocyte transendothelial migratory potential, peripheral neutrophil infiltration, and bbb breakdown additional immunohistochemical staining for pecam- mpo, and igg was performed to identify leukocyte transendothelial migratory potential, peripheral neutrophil infiltration, and bbb breakdown, respectively. pecam- and mpo staining was only observed within injured brain regions. igg staining was diffusely present throughout the ipsilateral hemisphere and crossed into the contralateral hemisphere in some cases. quantification showed that 17-pt-pge2-treated mice had  ±  % less pecam- immunoreactivity when compared to the control group. likewise, these mice also had  ±  % less neutrophil infiltration and  ±  % less igg immunoreactivity. no significant differences in pecam- mpo, or igg immunoreactivity were observed for the sc-51089-treated group.fig.  pecam- mpo, and igg immunoreactivity following ich and blockade or stimulation of the ep receptor. antagonist, agonist, or vehicle was administered subcutaneously at the onset of injury, 6 h post-ich, and at 12-h intervals thereafter. seventy-two hours after ich, brains were harvested and sections processed for pecam- mpo, and igg immunohistochemistry to evaluate leukocyte transendothelial migratory potential, neutrophil infiltration, and bbb breakdown, respectively. a, b, c representative high magnification photomicrographs showing a pecam- staining highlighting blood vessels within the lesion, b mpo-positive neutrophils within the lesion, and c igg staining within the lesion and surrounding areas of coronal brain sections from control, sc-51089-, and 17-pt-pge2- treated mice. square selections in the insets denote magnified regions. scale bars on the magnified images and inserts are 100 µm and 2 mm, respectively. d, e, f quantification revealed that 17-pt-pge2-treated mice had significantly less d pecam- staining, e neutrophil infiltration, and f bbb breakdown. all quantification data are presented at the same scale such that the relative d pecam- e mpo, and f igg immunoreactivity can be directly compared. no significant differences were seen for the sc-51089-treated mice. *p <  and **p <  when compared to the control group, n = 7– per group. discussion we have shown that chronic post-treatment with sc- a selective ep receptor antagonist, aggravates brain injury after ich, resulting in larger lesion volumes and more iron accumulation, whereas treatment with 17-pt-pge an ep receptor agonist with extensive ep receptor agonism, results in no detectable changes in lesion volume or iron deposition, but improves neuromuscular functional recovery. the two treatments resulted in differential patterns of microglial and astrocyte activation, with ep antagonism producing increased cortical microgliosis and ep1/ep agonism resulting in reduced cortical and striatal astrogliosis. last, treatment with the ep agonist reduced leukocyte transendothelial migratory potential, neutrophil infiltration, and bbb breakdown. our results demonstrating that post-ich ep antagonism exacerbates brain injury are in agreement with our previous study that showed significantly more ich-induced brain injury and worse functional outcomes in mice with genetic deletion of the ep receptor, although we did not observe smaller lesion volumes with the ep agonist used in this study, 17-pt-pge this ep agonist has ki values of and  nm for the ep and ep receptors, respectively, and the non-selective nature could have led to the outcomes seen here. in support of this hypothesis, we have recently shown that ep3−/− mice have less ich-induced brain injury when compared to wt controls, implying an injurious role for pge2-ep signaling that is opposite to the neuroprotective effects of the ep receptor. therefore, it is likely that the overall desired beneficial effect of 17-pt-pge by agonism of the ep receptor is negated by simultaneous ep receptor agonism, resulting in no observed differences with 17-pt-pge treatment when compared to the control group. it is also possible that the dose used here for agonist treatment may not be high enough to affect lesion volume after ich. interestingly, the 17-pt-pge2-treated mice performed better on neurobehavioral testing, including grip strength examination and the hanging wire task. in our aforementioned observations with ep3−/− mice, we did not find the same extent of differences on neurobehavioral testing as compared to ep1−/− mice, and thus collectively these data imply an important role for the pge2-ep signaling axis in post-ich functional recovery. additional studies with 17-pt-pge treatment in the appropriate ep receptor knockout animals would be necessary to verify this suggestion. furthermore, studies in other ich models and using additional selective ep receptor agonists, with a dose-response curve and multiple endpoints, will clarify the therapeutic potential of targeting the ep receptor for the treatment of ich. nevertheless, the main finding that post-treatment with a selective ep antagonist aggravates ich-induced brain injury supports our previous findings suggesting an important role for the ep receptor in positively modulating ich outcomes. many studies have shown differential effects of modulating pge2-ep receptor signaling on outcomes in models of acute and chronic neurologic disorders, including contradictory results in the various stroke models. it appears that ep receptor agonism and antagonism may be useful under hemorrhagic and ischemic conditions, respectively. hemolysis following ich is an important trigger in the progression of brain injury. the liberated free heme generates ferric ions, which via the fenton reaction produce reactive oxygen species, inducing oxidative stress. to understand whether ep receptor activation or inhibition modulates ferric iron content, brain sections were subjected to perl’s iron staining. analogous to the effects seen with sc- and 17-pt-pge treatment on lesion volume, we found an increase in the ferric iron content of antagonist-treated mice, while no significant differences were observed in the agonist-treated group. these data suggest that inhibition of pge2-ep receptor signaling prevents the clearance of iron from injured brain regions, which may be a consequence of impaired microglial phagocytosis with ep blockade since deletion of this receptor has been reported to reduce phagocytic capability. however, another possible explanation is that antagonist-treated mice have more bleeding, leading to more accumulation of iron in the hemorrhagic area. the latter hypothesis is supported by our previous findings indicating that deletion of the ep receptor increases cerebral blood flow. microglial and astrocyte activation is another criterion to gauge injury severity and monitor the neuroinflammatory response. following ich, microglia likely play a dual role where they are protective in their cleanup of red blood cells and damaged brain tissue, thereby promoting repair, but toxic in their facilitation of neuroinflammation and recruitment of leukocytes through the production of proinflammatory cytokines. here, significantly increased microgliosis was seen in ipsilateral cortical regions with ep antagonist treatment, whereas no differences were seen in the ipsilateral striatal regions of the same group or in the agonist-treated group. we have previously shown that the ep receptor colocalizes with microglial markers in perihematomal regions and that receptor deletion reduces the activated microglial population and phagocytic function. several possibilities exist to explain these contradictory striatal microgliosis findings in ep1−/− mice and wt mice treated with an ep antagonist: prostaglandin receptors are reported to cross talk, and it is possible that ep deletion caused the expression of another prostanoid receptor important for regulating microglial activation state to change, deletion of the ep receptor reduces basal levels of microglial activation, and ep1−/− mice may develop some other compensatory mechanism. in comparing cortical and striatal post-ich microglial activation state with ep antagonism, cortical microglia appear to retain their ability to activate and respond to the injury, indicating a brain region-specific response. astrocytes play diverse roles in response to brain injury, where they try to contain the damage be forming a glial scar. however, excessive or sustained astrocyte activation can be detrimental and augment brain damage and functional deficits by contributing to chronic inflammation and cell death. treatment with the antagonist had no effect on astrogliosis, whereas the agonist significantly reduced astrogliosis in the ipsilateral cortical and striatal regions. strong evidence exists suggesting that impairment of astrogliosis improves axonal growth and improves functional outcomes following brain injury. here, we observed a significant decrease in cortical and striatal astrogliosis in the agonist-treated group, whereas no significant differences were seen with ep antagonist treatment. intriguingly, this reduced astrogliosis with 17-pt-pge treatment was accompanied by better neuromuscular functional recovery. the discrepancy in astrocyte and microglial activation with ep antagonism or agonism may also result from the unbalanced influence of pge2-ep receptor signaling between the treatment groups. finally, we evaluated leukocyte transendothelial migratory potential, leukocyte infiltration, and bbb disruption, where we found less immunoreactivity for markers of these outcome measures in the agonist-treated group and no significant differences with ep antagonism. as previously mentioned in the context of lesion volume, neurobehavioral testing, and gliosis, the interpretation of these data is complicated given the lack of opposing results between the two treatment groups and non-selective nature of the agonist toward ep1/ep receptors; although, it does suggest that the ep receptor may play a larger role in modulating these particular outcomes when compared to the contribution of pge2-ep signaling. ich initially directly disrupts the bbb, leading to a massive neuroinflammatory cascade that is characterized by invasion of the lesion cite by leukocytes and further damage to the vasculature. there are various triggers that can lead to secondary bbb dysfunction after ich. importantly, blood products and the inflammatory response, including that mediated by endogenous and infiltrating cells, play a critical role in ich-induced bbb dysfunction. as previously mentioned, we found that treatment with the ep antagonist increased brain ferric iron content, whereas the ep agonist had no effect. higher iron load after ich means a greater potential for oxidative damage and inflammation, ultimately leading to more bbb damage. however, we did not observe an increase in bbb dysfunction in the antagonist-treated group; instead, we observed improved bbb integrity in the agonist-treated mice that did not display less ferric iron content or smaller lesion volumes. these findings suggest that in the present study, cell-mediated inflammatory responses may be primarily responsible for the observed differences in bbb integrity between the treatment groups. endogenous cell types such as microglia and astrocytes can secrete factors that modulate bbb permeability, especially with excessive or sustained activation. here, we have shown that agonist-treated mice display significantly reduced activation of one of the key cell types responsible for maintenance of the bbb, astrocytes, and this reduced astrogliosis could be contributing to the overall preservation of bbb integrity with 17-pt-pge treatment. however, infiltrating leukocytes such as neutrophils migrate to the site of injury and augment the neuroinflammatory cascade, causing further damage to the bbb. our findings show that treatment with 17-pt-pge decreases neutrophil infiltration, as monitored by immunohistochemical staining for mpo. in the current experimental setting, it is not possible to distinguish the respective contribution of astrogliosis and leukocyte infiltration in modulating bbb integrity, although both processes seem to play a role. leukocytes cross the endothelium using paracellular and transcellular pathways. pecam- is expressed on the surface of several types of leukocytes and lateral borders of endothelial cells, and is involved in the transmigration phase of leukocyte emigration through homophilic interactions. neutrophil transmigration capability, as tested by pecam- staining, was found to be lower in mice treated with 17-pt-pge implying that ep1/ep agonism preserves the bbb barrier and reduces leukocyte infiltration by sealing intercellular gaps between endothelial cells, and probably more importantly, decreasing the engagement of migrating leukocytes.  in this study we have provided additional evidence consistent with our previous findings suggesting a neuroprotective role for the ep receptor following ich. our results show that blockade of the pge2-ep signaling axis aggravates ich-induced brain injury and increases ferric iron deposition and microgliosis, whereas stimulation decreases astrogliosis, leukocyte transendothelial migratory potential, neutrophil infiltration, and bbb breakdown and improves neuromuscular functional recovery. caution should be used when interpreting and extrapolating these results because it is likely that a portion of the findings are due to activation of ep signaling rather than ep given the non-selective nature of the agonist used in this study. even so, ep1-mediated post-ich neuroprotection is probably achieved by positive modulation of microglial phagocytosis and/or decreased bleeding tendency, leading to lower iron overload, less oxidative processes, and attenuated neuroinflammatory responses. additional studies with other ich models, a selective ep agonist, and pharmacologic manipulation of the ep receptor will clarify the respective role of these pge2-mediated signaling axes after ich. nonetheless, the ep receptor remains a viable therapeutic target for the treatment of ich. abbreviations abcavidin/biotin enzyme complex adalzheimer’s disease bbbblood brain barrier campcyclic adenosine monophosphate coxcyclooxygenase dab ′-diaminobenzidine dmsodimethyl sulfoxide ep1e prostanoid receptor subtype 1 ep3e prostanoid receptor subtype 3 ep1-/−ep receptor knockout gfapglial fibrillary acidic protein hiehypoxic-ischemic encephalopathy iba1ionized calcium-binding adapter protein 1 ichintracerebral hemorrhage iggimmunoglobulin g mpgesmembrane-bound pge synthase mpomyeloperoxidase pbsphosphate-buffered saline pdparkinson’s disease pecam-1platelet endothelial cell adhesion molecule 1 pfaparaformaldehyde pge2prostaglandin e2 semstandard error of the mean tbitraumatic brain injury wtwildtype jenna l leclerc and abdullah s ahmad contributed equally to this work authors’ contributions jll, ass, and sd designed the study, interpreted results, and wrote the manuscript. jll coordinated histology and blinded quantification, and analyzed data; ns performed surgical procedures, coordinated blinded behavioral testing, and assisted with histology; ls performed behavioral analyses; ls, eg, and ad contributed to histological staining, quantification, and data analysis. all authors read and approved the final manuscript. 
we have previously shown that ep receptor deletion aggravates ich-induced brain injury and impairs functional recovery, thus the current study aimed to elaborate on these results by including a pharmacologic approach targeting the ep receptor. in agreement with our previous results, selective antagonism of the ep receptor aggravated ich-induced brain injury. inflammation following intracerebral hemorrhage significantly contributes to secondary brain damage and poor outcomes. following ich, mice treated with the antagonist displayed increased ferric iron and cortical microgliosis, whereas treatment with the agonist decreased cortical and striatal astrogliosis, leukocyte transendothelial migratory potential, neutrophil infiltration, and blood brain barrier breakdown. chronic post-treatment with the selective ep receptor antagonist, sc- increased lesion volume by  ±  % and treatment with the ep agonist, 17-pt-pge improved neuromuscular functional recovery on grip strength and hanging wire behavioral testing. inhibition of cox- activity has been reported to attenuate ich-induced brain injury; however, the clinical utility of such drugs is limited due to the potential for severe side effects. to begin identifying the mechanisms involved in ep1-mediated neuroprotection after ich, histology was performed to assess ferric iron content, neuroinflammation, leukocyte transendothelial migratory potential, and peripheral neutrophil and immunoglobulin infiltration. furthermore, ep receptor agonism improved anatomical outcomes and functional recovery. prostaglandin e is known to modulate neuroinflammatory responses and is upregulated in response to brain injury as a result of changes in inducible cyclooxygenase and the membrane-bound type of pge synthase.
 inflammation following intracerebral hemorrhage significantly contributes to secondary brain damage and poor outcomes. prostaglandin e is known to modulate neuroinflammatory responses and is upregulated in response to brain injury as a result of changes in inducible cyclooxygenase and the membrane-bound type of pge synthase. inhibition of cox- activity has been reported to attenuate ich-induced brain injury; however, the clinical utility of such drugs is limited due to the potential for severe side effects. therefore, it is now important to search for downstream targets capable of preferentially modulating pge signaling, and the four e prostanoid receptors, ep1- which are the main targets of pge remain a viable therapeutic option. we have previously shown that ep receptor deletion aggravates ich-induced brain injury and impairs functional recovery, thus the current study aimed to elaborate on these results by including a pharmacologic approach targeting the ep receptor.  chronic post-treatment with the selective ep receptor antagonist, sc- increased lesion volume by  ±  % and treatment with the ep agonist, 17-pt-pge improved neuromuscular functional recovery on grip strength and hanging wire behavioral testing. to begin identifying the mechanisms involved in ep1-mediated neuroprotection after ich, histology was performed to assess ferric iron content, neuroinflammation, leukocyte transendothelial migratory potential, and peripheral neutrophil and immunoglobulin infiltration. following ich, mice treated with the antagonist displayed increased ferric iron and cortical microgliosis, whereas treatment with the agonist decreased cortical and striatal astrogliosis, leukocyte transendothelial migratory potential, neutrophil infiltration, and blood brain barrier breakdown.  in agreement with our previous results, selective antagonism of the ep receptor aggravated ich-induced brain injury. furthermore, ep receptor agonism improved anatomical outcomes and functional recovery. thus, the present data continues to reinforce a putative role for ep as a new and more selective therapeutic target for the treatment of ich that could reduce the side effects associated with cox- inhibition while still exploiting the beneficial effects. keywords 17-pt-pge2ep3gliosisironneuroinflammationneuroprotectionpecamsc-51089strokeissue-copyright-statement© the author 2015 