BACKGROUND
the ability to reliably engineer a protein that spans the plasma membrane  allows synthetic control over the communication of the cell with its extracellular environment  <cit> .
the intercellular and extracellular environment is separated by the pm, where proteins spanning the pm facilitate diverse cellular processes such as signal transduction, cell-cell contact and molecular transport  <cit> .
many proteins spanning the pm contain an n-terminal signal peptide that is recognized by the signal recognition particle  and recruited to the ribosomes on the endoplasmic reticulum  for translation  <cit> .
subsequently the signal peptide is cleaved off by the signal peptidase.
signal peptides are usually  <dig> to  <dig> amino acids consisting of initially, a hydrophilic segment followed by a hydrophobic segment and finally a signal peptidase cleavage site  <cit> .
proteins spanning the pm require at least one transmembrane domain  consisting of mostly hydrophobic residues, that at first, anchors the protein to the er membrane, among other retention signals.
next, the pm-bound proteins are packaged into coated vesicles  and shuttled from the er to the golgi apparatus for further processing  <cit> .
upon exiting the golgi apparatus, they are again packaged into vesicles  and shuttled to the pm  <cit> .
after the membrane of the vesicle fuses with the pm, the membrane-spanning protein is delivered to the pm.
while many groups have created membrane-tethered proteins for use as research tools and therapeutics  <cit> , it is unclear what the minimum necessary components to engineer a protein that spans the pm are.
examples of membrane-tethered proteins from other groups include antibody fragments comprising pseudo-receptors to activate the immune system  <cit> , secreted cytokines to induce specific antitumor immune responses with reduced systemic toxicity  <cit>  and enzymes to activate prodrugs  <cit> .
to enhance cell surface expression of membrane-tethered proteins, design strategies have focused on the careful selection of the tm, the cytoplasmic tail and the extracellular juxtamembrane linker  <cit> .
elements such as the transmembrane domain and the cytoplasmic tail of the murine b7- <dig> antigen and the hinge-ch2-ch <dig> region of the human immunoglobulin g  heavy chain have been established to enhance protein expression on the cell surface  <cit> .
while the minimum sequences required to target specific proteins such as the respiratory syncytial virus f protein  <cit>  and the snare protein snap- <dig>  <cit>  have been elucidated in independent studies previously, these specific minimum sequences could differ between target proteins.
to determine the minimum necessary components to engineer a protein that spans the pm with a defined orientation, we used a modular synthetic biology approach to assemble the protein.
previously, our group employed a similar approach to determine that the minimum component to engineer a protein that spans the er is a single tm at an arbitrary location in the protein  <cit> .
an n-terminal signal peptide was not necessary to deliver the protein to the er  <cit> .
here, we confirm that while an n-terminal tm directs the protein for translation in the er, the engineered protein is still strongly retained in the er.
the addition of a downstream peptidase cleavage site to this tm further enhances the exit of the engineered protein from the er, thereby decreasing its retention in the er.
finally, a second tm strongly increases retention of the engineered protein in the pm.
furthermore, the orientation  of the engineered proteins were determined by fluorescence quenching and detection of intracellular ca2+ signaling.
RESULTS
a signal peptidase cleavage site enhances sorting out of the er
while an n-terminal tm traffics the protein to the er, an additional signal peptidase cleavage site downstream of this tm enhanced sorting out of the er.
the protein named tm-venus was created as the tandem fusion of the tm from the human toll-like receptor  <dig>   and the yellow fluorescent protein  mutant venus  <cit>  .
although the tm domain from tlr <dig> was used in this study, tm domains from other proteins should behave similarly because in our previous work  <cit> , the tm domain from platelet-derived growth factor receptor behaved indistinguishably to tlr <dig>  to label the plasma membrane, the protein lyn-ceru was used consisting of the tandem fusion of n-terminal localization sequence of lyn kinase  and the cyan fluorescent protein variant cerulean  <cit> .
following post-translational modification , lyn-ceru localizes to the cytoplasmic side of the plasma membrane.
likewise, to label the er, the protein stim1-mrfp was used, consisting of the tandem fusion of the stromal interaction molecule  <dig>  and the monomeric red fluorescent protein   <cit> .
mammalian cells were co-transfected with tm-venus and lyn-ceru to compare localization with the pm or tm-venus and stim1-mrfp to compare localization with the er .
tm-venus showed a web-like fluorescence distribution similar to stim1-mrfp as would be expected from er localization  =  <dig>  ±  <dig> )  .
in contrast, the tm-venus had a starkly different fluorescence distribution compared to lyn-ceru that labels the plasma membrane .
there was a significant difference between er and plasma membrane localization .
notably, lyn-ceru labels the pm distinctly depending on cell spreading.
cells that spread well  show a matte-like fluorescence appearance, whereas cells that do not spread as well ) often exhibit a fluorescence that outlines the boundary of the cell .
to enhance sorting out of the er, the protein named tlp-venus was created as the tandem fusion of tm, the signal peptidase cleavage site from human immunoglobulin k  and venus.
cells were co-transfected with tlp-venus and either lyn-ceru or stim1-mrfp.
tlp-venus had a less web-like fluorescence distribution when compared to stim1-mrfp    and while it did not look plasma membrane when measured against lyn-ceru  either, it improved sorting compared to the tm-venus case  .
repeated trials in another mammalian cell line  yielded similar results  .
thus, the signal peptidase cleavage site enhanced sorting out of the er.fig.
<dig> a signal peptidase cleavage site enhances sorting out of the er.
schematic layout of fusion proteins constructed in the study .
pictorial representation of receptors exiting the er .
cos- <dig> cells transfected with the plasma membrane labelled lyn-ceru showed a matte-like appearance  while those transfected with the er labelled stim1-mrfp showed a web-like fluorescence distribution .
tm-venus showed a web-like fluorescence distribution similar to stim1-mrfp .
merged images illustrate resultant co-localization .
cos- <dig> cells transfected with tlp-venus  had a less web-like fluorescence distribution when compared to stim1-mrfp .
merged images illustrate resultant co-localization .
amino acid single letter codes pgstgd represent proline, glycine, serine, threonine, glycine and aspartic acid, respectively.
tm: transmembrane domain tlr <dig>  tlp: fusion of tm with signal peptidase cleavage site from human immunoglobulin k, rgeco: red fluorescent genetically encoded ca2+ indicator, er: endoplasmic reticulum, lc: lyn-ceru, stim1: stromal interaction molecule  <dig>  scale bars are 10 μm.
images are false colored: cfp, cyan; yfp, green; mrfp, red.
all insets show zoomed regions  of structures in dotted rectangles.
all experiments were repeated at least  <dig> times
pccs of fusion proteins with markers lc  and stim <dig> localized to the pm and er, respectively
aaverage pccs calculated from  <dig> independent experiments with at least  <dig> cells per experiment
bratio of pm accumulation calculated as: =ratiolc:fusionprotein−ratiolc:stim1ratiolc:lc−ratiolc:stim <dig>  where ratio  =  <dig>  and ratio  = 1
a second tm anchors the protein on the pm
while tlp-venus enhances sorting out of the er, a second tm helps anchor and accumulate proteins on the pm .
to anchor proteins on the plasma membrane, the construct tlp-venus-tm was created.
proteins sorted from the er are directed via cop ii vesicles to the trans golgi network  for further processing before being packaged into recycling endosomes  or post-golgi transport intermediates  for delivery to the plasma membrane  <cit> .
from the tgn, proteins are committed to one of two secretory pathways: constitutive secretion involves the continuous release of proteins from the cell whereas regulated secretion retains proteins intracellularly before an external signal initiates release  <cit> .
ultimately, the secretory vesicle fuses with the pm and the protein is either to be secreted or retained on the plasma membrane  <cit> .
it is plausible that the presence of an anchoring domain  on the protein will enhance retention of the protein on the pm.
in theory, since the tlp contains a signal peptidase cleavage site, the first tm within tlp cannot serve as permanent anchoring domain, however the second tm can serve in that function.
when tlp-venus-tm was co-transfected with either lyn-ceru  or stim1-mrfp   in cos- <dig> cells, the protein distribution skewed more towards the plasma membrane as expected  .
the matte-like fluorescence was significantly similar to that of lyn-ceru unlike the web-like detail seen in stim1-mrfp  .
repeated trials in another mammalian cell line  yielded similar results  .
to estimate the relative degree of pm accumulation between tm-venus, tlp-venus and tlp-venus-tm, we compared their co-localization to stim1-mrfp and lyn-ceru in the same cell .
if the co-localization is the same as stim1-mrfp, the ratio of pm accumulation is 0; if similar to lyn-ceru,  <dig>  the ratio of pm accumulation for tm-venus, tlp-venus and tlp-venus-tm was calculated as  <dig> ,  <dig> , and  <dig> , respectively, suggesting each modification increased accumulation to the pm.fig.
<dig> an additional transmembrane domain anchors proteins on the pm with the fluorescent protein, venus, facing extracellularly.
pictorial representation of receptor anchoring and orientation .
cos- <dig> cells transfected with the plasma membrane labelled lyn-ceru showed a matte-like appearance  while those transfected with the er labelled stim1-mrfp showed a web-like fluorescence distribution .
merged images illustrate resultant co-localization .
tlp-v-tm skewed more towards a matte-like plasma membrane appearance .
fluorescence quenching with f hcl showed no effect on membrane-labelled lyn-ceru at time =  <dig> and time = 1 min  but did sequester venus .
likewise, fluorescence quenching with f nacl showed no effect on membrane-labelled lyn-ceru at time =  <dig> and time = 1 min  but again sequestered venus .
er: endoplasmic reticulum, pm: plasma membrane, hv: emitted fluorescent light, hcl: hydrogen chloride, nacl: sodium chloride, tm: transmembrane domain tlr <dig>  tlp: fusion of tm with signal peptidase cleavage site from human immunoglobulin k, v: venus fluorescent protein, lc: lyn-ceru, stim1: stromal interaction molecule  <dig>  scale bars are 10 μm.
images are false colored: cfp, cyan; yfp, green; mrfp, red.
all insets show zoomed regions  of structures in dotted rectangles.
all experiments were repeated at least  <dig> times
to compare the rate of trafficking of tm-venus, tlp-venus and stim1-mrfp to the pm, we performed a cyclohexamide chase assay in cho cells.
since cyclohexamide inhibits protein synthesis, we can determine protein accumulation on the pm over time by observing the co-localization with lyn-ceru.
at time 0 h, both tm-venus and stim1-mrfp had lower and similar pcc with lyn-ceru  whereas tlp-venus had a higher and statistically different pcc with lyn-ceru  .
at time  <dig>   <dig> and 12 h, cyclohexamide enhanced accumulation of both tm-venus, tlp-venus to the pm at a similar rate, but as expected did not enhance accumulation of stim1-mrfp to the pm .
it is worth noting that in our previous study  <cit> , we created proteins of the tm-fluorescent protein-tm architecture where the fluorescence was mostly er, so the signal cleavage site is needed for enhanced exiting of the er.
venus faces the extracellular side in the tlp-venus-tm construct
to determine the orientation of tlp-venus-tm, fluorescence quenching was performed using saturating acidic and salt conditions.
under saturated acidic conditions, rapid  protonation of the protein backbone physically expands and thus denatures the barrel-like domain housing fluorescence-producing chromophore phenols.
this makes the chromophores also susceptible to protonation leading to a decrease in fluorescence  <cit> .
fluorescence quenching is also seen with an increased salt concentration which reduces the repulsion between surface charges on the protein causing it to collapse via van der waals forces  <cit> .
since transport through ion channels is often needed to influx these stimuli , they do not freely permeate the hydrophobic pm bilayer due to charge constraints.
within a minute of treatment with either f of hcl or f of nacl, a change in the expected fluorescence distribution of tlp-venus- tm was observed   .
noticeably, only the plasma membrane localized protein  was quenched as it was accessible to the quenching agents while those proteins being processed intracellularly remained intact.
at time =  <dig>  the fluorescence appears strongly matte-like while within a minute post treatment, this trait vanishes and a previously masked fluorescence of the intracellular proteins being processed becomes clearly visible.
since the fluorescence distribution of lyn-ceru which is facing the cytoplasmic side of the pm remains identical pre- and post-treatment, venus in the tlp-venus-tm construct should be extracellular  .
an ancillary fragment, r-geco faces the cytoplasmic side in the tlp-venus-tm-r-geco
a protein domain fused c-terminal to tlp-venus-tm faces the cytoplasm.
to determine the orientation of this domain, the construct tlp-venus-tm-r-geco was created, where r-geco is a red fluorescent genetically encoded ca2+ indicator  <cit>  .
the extracellular ca2+ concentration in the media is approximately 2 mm while the intracellular concentration ranges from  <dig>  to 1 μm - a difference of a thousand times.
r-geco being sensitive to the smaller range would not be responsive to any intracellular ca2+ transient if it were facing extracellularly  <cit> .
co-transfection of tlp-venus-tm-rgeco and lyn-ceru in mammalian cells confirmed its localization to the plasma membrane as indicated by the cell’s matte-like appearance   .
the red fluorescence from r-geco was initially weak but increased in intensity in response to a ca2+ transient.
upon stimulation with 10 μm atp, the ca2+ concentration rapidly increased to a peak within a few seconds and then gradually declined to its basal level within two to three minutes  .
it has been established that atp activates endogenous p2y receptors in cells to stimulate ip <dig> production and subsequent ca2+ release from the endoplasmic reticulum  <cit> .
the measured ca2+ response shows that the domain following the tm was intracellular.fig.
<dig> an ancillary fragment, r-geco, is cytoplasmic and responsive to an induced ca2+ influx.
pictorial representation of receptor orientation and response .
cos- <dig> cells transfected with the plasma membrane labelled lyn-ceru showed a matte-like appearance  while those transfected with the er labelled stim1-mrfp showed a web-like fluorescence distribution .
tlp-v-tm-rgeco showed a matte-like fluorescence distribution similar to lyn-ceru .
merged images illustrate resultant co-localization .
upon stimulation with 10 μm atp, the ca2+ concentration rapidly increased to a peak within a few minutes and then gradually declined to its basal level within two to three minutes .
er: endoplasmic reticulum, pm: plasma membrane, atp: adenosine triphosphate, tm: transmembrane domain tlr <dig>  tlp: fusion of tm with signal peptidase cleavage site from human immunoglobulin k, v: venus fluorescent protein, rgeco: red fluorescent genetically encoded ca2+ indicator, lc: lyn-ceru, stim1: stromal interaction molecule  <dig>  scale bars are 10 μm.
images are false colored: cfp, cyan; yfp, green; mrfp, red.
all insets show zoomed regions  of structures in dotted rectangles.
all experiments were repeated at least  <dig> times
CONCLUSIONS
using a modular synthetic biology approach to assemble proteins, we verify that proteins are targeted to the er membrane with the inclusion of an n-terminal tm.
with the addition of a peptidase cleavage site downstream of the tm, the protein has enhanced exit from the er; with the further addition of a second tm, the protein can efficiently anchor and accumulate on the pm.
furthermore, fluorescence quenching and intracellular ca2+ responses were used to distinguish domain orientation.
this study establishes a foundation to engineer novel synthetic proteins that span the pm that can be used to potentially direct intracellular signaling in response to changing extracellular conditions.
for instance, extracellular domains could be selected to dimerize in response to a synthetic molecule, which in turn cause specific intracellular kinase domains to autophosphorylate and activate desired downstream signaling cascades.

 the ability to reliably engineer a protein that spans the plasma membrane allows synthetic control over the communication of the cell with its extracellular environment. the intercellular and extracellular environment is separated by the pm, where proteins spanning the pm facilitate diverse cellular processes such as signal transduction, cell-cell contact and molecular transport. many proteins spanning the pm contain an n-terminal signal peptide that is recognized by the signal recognition particle and recruited to the ribosomes on the endoplasmic reticulum for translation. subsequently the signal peptide is cleaved off by the signal peptidase. signal peptides are usually to amino acids consisting of initially, a hydrophilic segment followed by a hydrophobic segment and finally a signal peptidase cleavage site. proteins spanning the pm require at least one transmembrane domain consisting of mostly hydrophobic residues, that at first, anchors the protein to the er membrane, among other retention signals. next, the pm-bound proteins are packaged into coated vesicles and shuttled from the er to the golgi apparatus for further processing. upon exiting the golgi apparatus, they are again packaged into vesicles and shuttled to the pm. after the membrane of the vesicle fuses with the pm, the membrane-spanning protein is delivered to the pm. while many groups have created membrane-tethered proteins for use as research tools and therapeutics, it is unclear what the minimum necessary components to engineer a protein that spans the pm are. examples of membrane-tethered proteins from other groups include antibody fragments comprising pseudo-receptors to activate the immune system, secreted cytokines to induce specific antitumor immune responses with reduced systemic toxicity and enzymes to activate prodrugs. to enhance cell surface expression of membrane-tethered proteins, design strategies have focused on the careful selection of the tm, the cytoplasmic tail and the extracellular juxtamembrane linker. elements such as the transmembrane domain and the cytoplasmic tail of the murine b7- antigen and the hinge-ch2-ch region of the human immunoglobulin g heavy chain have been established to enhance protein expression on the cell surface. while the minimum sequences required to target specific proteins such as the respiratory syncytial virus f protein and the snare protein snap- have been elucidated in independent studies previously, these specific minimum sequences could differ between target proteins. to determine the minimum necessary components to engineer a protein that spans the pm with a defined orientation, we used a modular synthetic biology approach to assemble the protein. previously, our group employed a similar approach to determine that the minimum component to engineer a protein that spans the er is a single tm at an arbitrary location in the protein. an n-terminal signal peptide was not necessary to deliver the protein to the er. here, we confirm that while an n-terminal tm directs the protein for translation in the er, the engineered protein is still strongly retained in the er. the addition of a downstream peptidase cleavage site to this tm further enhances the exit of the engineered protein from the er, thereby decreasing its retention in the er. finally, a second tm strongly increases retention of the engineered protein in the pm. furthermore, the orientation of the engineered proteins were determined by fluorescence quenching and detection of intracellular ca2+ signaling.  a signal peptidase cleavage site enhances sorting out of the er while an n-terminal tm traffics the protein to the er, an additional signal peptidase cleavage site downstream of this tm enhanced sorting out of the er. the protein named tm-venus was created as the tandem fusion of the tm from the human toll-like receptor and the yellow fluorescent protein mutant venus. although the tm domain from tlr was used in this study, tm domains from other proteins should behave similarly because in our previous work, the tm domain from platelet-derived growth factor receptor behaved indistinguishably to tlr to label the plasma membrane, the protein lyn-ceru was used consisting of the tandem fusion of n-terminal localization sequence of lyn kinase and the cyan fluorescent protein variant cerulean. following post-translational modification, lyn-ceru localizes to the cytoplasmic side of the plasma membrane. likewise, to label the er, the protein stim1-mrfp was used, consisting of the tandem fusion of the stromal interaction molecule and the monomeric red fluorescent protein. mammalian cells were co-transfected with tm-venus and lyn-ceru to compare localization with the pm or tm-venus and stim1-mrfp to compare localization with the er. tm-venus showed a web-like fluorescence distribution similar to stim1-mrfp as would be expected from er localization  =   ±  ). in contrast, the tm-venus had a starkly different fluorescence distribution compared to lyn-ceru that labels the plasma membrane. there was a significant difference between er and plasma membrane localization. notably, lyn-ceru labels the pm distinctly depending on cell spreading. cells that spread well show a matte-like fluorescence appearance, whereas cells that do not spread as well ) often exhibit a fluorescence that outlines the boundary of the cell. to enhance sorting out of the er, the protein named tlp-venus was created as the tandem fusion of tm, the signal peptidase cleavage site from human immunoglobulin k and venus. cells were co-transfected with tlp-venus and either lyn-ceru or stim1-mrfp. tlp-venus had a less web-like fluorescence distribution when compared to stim1-mrfp and while it did not look plasma membrane when measured against lyn-ceru either, it improved sorting compared to the tm-venus case. repeated trials in another mammalian cell line yielded similar results. thus, the signal peptidase cleavage site enhanced sorting out of the er.fig. a signal peptidase cleavage site enhances sorting out of the er. schematic layout of fusion proteins constructed in the study. pictorial representation of receptors exiting the er. cos- cells transfected with the plasma membrane labelled lyn-ceru showed a matte-like appearance while those transfected with the er labelled stim1-mrfp showed a web-like fluorescence distribution. tm-venus showed a web-like fluorescence distribution similar to stim1-mrfp. merged images illustrate resultant co-localization. cos- cells transfected with tlp-venus had a less web-like fluorescence distribution when compared to stim1-mrfp. merged images illustrate resultant co-localization. amino acid single letter codes pgstgd represent proline, glycine, serine, threonine, glycine and aspartic acid, respectively. tm: transmembrane domain tlr tlp: fusion of tm with signal peptidase cleavage site from human immunoglobulin k, rgeco: red fluorescent genetically encoded ca2+ indicator, er: endoplasmic reticulum, lc: lyn-ceru, stim1: stromal interaction molecule scale bars are 10 μm. images are false colored: cfp, cyan; yfp, green; mrfp, red. all insets show zoomed regions of structures in dotted rectangles. all experiments were repeated at least times pccs of fusion proteins with markers lc and stim localized to the pm and er, respectively aaverage pccs calculated from independent experiments with at least cells per experiment bratio of pm accumulation calculated as: =ratiolc:fusionprotein−ratiolc:stim1ratiolc:lc−ratiolc:stim where ratio  =  and ratio  = 1 a second tm anchors the protein on the pm while tlp-venus enhances sorting out of the er, a second tm helps anchor and accumulate proteins on the pm. to anchor proteins on the plasma membrane, the construct tlp-venus-tm was created. proteins sorted from the er are directed via cop ii vesicles to the trans golgi network for further processing before being packaged into recycling endosomes or post-golgi transport intermediates for delivery to the plasma membrane. from the tgn, proteins are committed to one of two secretory pathways: constitutive secretion involves the continuous release of proteins from the cell whereas regulated secretion retains proteins intracellularly before an external signal initiates release. ultimately, the secretory vesicle fuses with the pm and the protein is either to be secreted or retained on the plasma membrane. it is plausible that the presence of an anchoring domain on the protein will enhance retention of the protein on the pm. in theory, since the tlp contains a signal peptidase cleavage site, the first tm within tlp cannot serve as permanent anchoring domain, however the second tm can serve in that function. when tlp-venus-tm was co-transfected with either lyn-ceru or stim1-mrfp in cos- cells, the protein distribution skewed more towards the plasma membrane as expected. the matte-like fluorescence was significantly similar to that of lyn-ceru unlike the web-like detail seen in stim1-mrfp. repeated trials in another mammalian cell line yielded similar results. to estimate the relative degree of pm accumulation between tm-venus, tlp-venus and tlp-venus-tm, we compared their co-localization to stim1-mrfp and lyn-ceru in the same cell. if the co-localization is the same as stim1-mrfp, the ratio of pm accumulation is 0; if similar to lyn-ceru, the ratio of pm accumulation for tm-venus, tlp-venus and tlp-venus-tm was calculated as,, and, respectively, suggesting each modification increased accumulation to the pm.fig. an additional transmembrane domain anchors proteins on the pm with the fluorescent protein, venus, facing extracellularly. pictorial representation of receptor anchoring and orientation. cos- cells transfected with the plasma membrane labelled lyn-ceru showed a matte-like appearance while those transfected with the er labelled stim1-mrfp showed a web-like fluorescence distribution. merged images illustrate resultant co-localization. tlp-v-tm skewed more towards a matte-like plasma membrane appearance. fluorescence quenching with f hcl showed no effect on membrane-labelled lyn-ceru at time =  and time = 1 min but did sequester venus. likewise, fluorescence quenching with f nacl showed no effect on membrane-labelled lyn-ceru at time =  and time = 1 min but again sequestered venus. er: endoplasmic reticulum, pm: plasma membrane, hv: emitted fluorescent light, hcl: hydrogen chloride, nacl: sodium chloride, tm: transmembrane domain tlr tlp: fusion of tm with signal peptidase cleavage site from human immunoglobulin k, v: venus fluorescent protein, lc: lyn-ceru, stim1: stromal interaction molecule scale bars are 10 μm. images are false colored: cfp, cyan; yfp, green; mrfp, red. all insets show zoomed regions of structures in dotted rectangles. all experiments were repeated at least times to compare the rate of trafficking of tm-venus, tlp-venus and stim1-mrfp to the pm, we performed a cyclohexamide chase assay in cho cells. since cyclohexamide inhibits protein synthesis, we can determine protein accumulation on the pm over time by observing the co-localization with lyn-ceru. at time 0 h, both tm-venus and stim1-mrfp had lower and similar pcc with lyn-ceru whereas tlp-venus had a higher and statistically different pcc with lyn-ceru. at time and 12 h, cyclohexamide enhanced accumulation of both tm-venus, tlp-venus to the pm at a similar rate, but as expected did not enhance accumulation of stim1-mrfp to the pm. it is worth noting that in our previous study, we created proteins of the tm-fluorescent protein-tm architecture where the fluorescence was mostly er, so the signal cleavage site is needed for enhanced exiting of the er. venus faces the extracellular side in the tlp-venus-tm construct to determine the orientation of tlp-venus-tm, fluorescence quenching was performed using saturating acidic and salt conditions. under saturated acidic conditions, rapid protonation of the protein backbone physically expands and thus denatures the barrel-like domain housing fluorescence-producing chromophore phenols. this makes the chromophores also susceptible to protonation leading to a decrease in fluorescence. fluorescence quenching is also seen with an increased salt concentration which reduces the repulsion between surface charges on the protein causing it to collapse via van der waals forces. since transport through ion channels is often needed to influx these stimuli, they do not freely permeate the hydrophobic pm bilayer due to charge constraints. within a minute of treatment with either f of hcl or f of nacl, a change in the expected fluorescence distribution of tlp-venus- tm was observed. noticeably, only the plasma membrane localized protein was quenched as it was accessible to the quenching agents while those proteins being processed intracellularly remained intact. at time =  the fluorescence appears strongly matte-like while within a minute post treatment, this trait vanishes and a previously masked fluorescence of the intracellular proteins being processed becomes clearly visible. since the fluorescence distribution of lyn-ceru which is facing the cytoplasmic side of the pm remains identical pre- and post-treatment, venus in the tlp-venus-tm construct should be extracellular. an ancillary fragment, r-geco faces the cytoplasmic side in the tlp-venus-tm-r-geco a protein domain fused c-terminal to tlp-venus-tm faces the cytoplasm. to determine the orientation of this domain, the construct tlp-venus-tm-r-geco was created, where r-geco is a red fluorescent genetically encoded ca2+ indicator. the extracellular ca2+ concentration in the media is approximately 2 mm while the intracellular concentration ranges from to 1 μm - a difference of a thousand times. r-geco being sensitive to the smaller range would not be responsive to any intracellular ca2+ transient if it were facing extracellularly. co-transfection of tlp-venus-tm-rgeco and lyn-ceru in mammalian cells confirmed its localization to the plasma membrane as indicated by the cell’s matte-like appearance. the red fluorescence from r-geco was initially weak but increased in intensity in response to a ca2+ transient. upon stimulation with 10 μm atp, the ca2+ concentration rapidly increased to a peak within a few seconds and then gradually declined to its basal level within two to three minutes. it has been established that atp activates endogenous p2y receptors in cells to stimulate ip production and subsequent ca2+ release from the endoplasmic reticulum. the measured ca2+ response shows that the domain following the tm was intracellular.fig. an ancillary fragment, r-geco, is cytoplasmic and responsive to an induced ca2+ influx. pictorial representation of receptor orientation and response. cos- cells transfected with the plasma membrane labelled lyn-ceru showed a matte-like appearance while those transfected with the er labelled stim1-mrfp showed a web-like fluorescence distribution. tlp-v-tm-rgeco showed a matte-like fluorescence distribution similar to lyn-ceru. merged images illustrate resultant co-localization. upon stimulation with 10 μm atp, the ca2+ concentration rapidly increased to a peak within a few minutes and then gradually declined to its basal level within two to three minutes. er: endoplasmic reticulum, pm: plasma membrane, atp: adenosine triphosphate, tm: transmembrane domain tlr tlp: fusion of tm with signal peptidase cleavage site from human immunoglobulin k, v: venus fluorescent protein, rgeco: red fluorescent genetically encoded ca2+ indicator, lc: lyn-ceru, stim1: stromal interaction molecule scale bars are 10 μm. images are false colored: cfp, cyan; yfp, green; mrfp, red. all insets show zoomed regions of structures in dotted rectangles. all experiments were repeated at least times  using a modular synthetic biology approach to assemble proteins, we verify that proteins are targeted to the er membrane with the inclusion of an n-terminal tm. with the addition of a peptidase cleavage site downstream of the tm, the protein has enhanced exit from the er; with the further addition of a second tm, the protein can efficiently anchor and accumulate on the pm. furthermore, fluorescence quenching and intracellular ca2+ responses were used to distinguish domain orientation. this study establishes a foundation to engineer novel synthetic proteins that span the pm that can be used to potentially direct intracellular signaling in response to changing extracellular conditions. for instance, extracellular domains could be selected to dimerize in response to a synthetic molecule, which in turn cause specific intracellular kinase domains to autophosphorylate and activate desired downstream signaling cascades. 
the orientation of the components of the synthetic protein were determined through measuring intracellular ca2+ signaling using the r-geco biosensor and through measuring extracellular quenching of yellow fluorescent protein variants by saturating acidic and salt conditions. while a transmembrane domain fused to the n-terminus of a protein is sufficient to traffic it to the endoplasmic reticulum, an additional signal peptidase cleavage site downstream of this tm enhanced sorting out of the er. using a modular approach to assemble proteins, we identified the minimum necessary components required to engineer such membrane-spanning proteins with predictable orientation in mammalian cells. transmembrane proteinsmodular assemblyendoplasmic reticulumplasma membraneprotein engineeringsynthetic biologyhttp://dx.doi.org/ /501100000015canadian cancer society research institute701936truong kevin http://dx.doi.org/ /501100000038natural sciences and engineering research council of canada05322-14truong kevin issue-copyright-statement© the author 2016 to achieve synthetic control over how a cell responds to other cells or the extracellular environment, it is important to reliably engineer proteins that can traffic and span the plasma membrane. this work forms the basis of engineering novel proteins that span the plasma membrane to potentially control intracellular responses to extracellular conditions. next, a second tm in the synthetic protein helped anchor and accumulate the membrane-spanning protein on the plasma membrane.
 to achieve synthetic control over how a cell responds to other cells or the extracellular environment, it is important to reliably engineer proteins that can traffic and span the plasma membrane. using a modular approach to assemble proteins, we identified the minimum necessary components required to engineer such membrane-spanning proteins with predictable orientation in mammalian cells.  while a transmembrane domain fused to the n-terminus of a protein is sufficient to traffic it to the endoplasmic reticulum, an additional signal peptidase cleavage site downstream of this tm enhanced sorting out of the er. next, a second tm in the synthetic protein helped anchor and accumulate the membrane-spanning protein on the plasma membrane. the orientation of the components of the synthetic protein were determined through measuring intracellular ca2+ signaling using the r-geco biosensor and through measuring extracellular quenching of yellow fluorescent protein variants by saturating acidic and salt conditions.  this work forms the basis of engineering novel proteins that span the plasma membrane to potentially control intracellular responses to extracellular conditions. electronic supplementary material the online version of this article contains supplementary material, which is available to authorized users. keywords transmembrane proteinsmodular assemblyendoplasmic reticulumplasma membraneprotein engineeringsynthetic biologyhttp://dx.doi.org/ /501100000015canadian cancer society research institute701936truong kevin http://dx.doi.org/ /501100000038natural sciences and engineering research council of canada05322-14truong kevin issue-copyright-statement© the author 2016 