BACKGROUND
dietary restriction , also referred to as calorie restriction, is an intervention that extends life span and delays the onset of age-related phenotypes in nearly all eukaryotic organisms in which it has been tested  <cit> .
simplistically, it is defined as a significant reduction in dietary intake in the absence of malnutrition.
many different approaches can be used to achieve dr. in mice and rats for example, life span extension is observed by either reducing the amount of food consumed daily  or by imposing an intermittent fasting regimen  <cit> .
in addition to simply reducing the amount of food intake, the effects of altering dietary composition has also been examined in different organisms.
methionine-restricted mice  <cit>  and rats  <cit>  have an extended life span, suggesting that the nutritional composition of the diet can influence longevity in mammals.
in fruit flies, reducing either the yeast extract or sugar composition of the food supply extends life span, although yeast extract appears to have the greatest impact on longevity  <cit> .
restriction of amino acids also increases life span in flies  <cit> .
in yeast, reducing either the amount of glucose or amino acids in the growth media increases replicative life span  <cit> .
multiple methods for dr have been used in c. elegans, as in other model organisms  <cit> .
one commonly used method in c. elegans is a genetic model, mutation of eat- <dig>  the eat mutants were originally identified in a screen for defects in feeding behavior – they have reduced food consumption due to pharyngeal pumping defects  <cit> .
several alleles of eat- <dig>  increase life span, with longevity generally correlating with the degree to which pumping rate is decreased  <cit> .
life span extension from mutation of eat- <dig> is independent of the foxo-family transcription factor daf- <dig> and is additive with a longevity-enhancing allele of the insulin/igf-1-like receptor daf- <dig>  <cit> .
this has led to the generally accepted model that dr acts to modulate longevity in a genetic pathway distinct from insulin/igf-1-like signaling   <cit> .
as with any genetic model of dr, however, eat mutants are not suitable for certain studies.
the relative activity associated with different eat- <dig> alleles has not been completely characterized, so it is unclear whether life span extension from dr is maximized in studies using these alleles.
in addition, eat mutants are food restricted from hatching, and dr during development may have secondary effects on adult physiology that are not fully understood.
dr by reducing food availability also increases life span in c. elegans.
the standard method for culturing c. elegans in the laboratory is to maintain the nematodes on the surface of nematode growth medium  nutrient agar with e. coli op <dig> as the food source.
dr on ngm nutrient agar has been achieved by reducing the amount of peptone in the media so as to limit bacterial growth  <cit>  or by reducing the amount of live bacteria present on the surface of the ngm nutrient agar  <cit> .
two methods of dr have also been described using non-standard, liquid-based growth conditions: bacterial dilution in s basal medium and axenic growth  <cit> .
both liquid-based methods increase life span relative to animals fed a diet of e. coli op <dig> in s basal, and axenic growth behaves similarly to mutation of eat- <dig> in epistasis experiments with iis  <cit> .
nonetheless, neither method is widely used for aging studies.
this may be due to the potential differences in growth under standard conditions on an agar surface versus growth in liquid culture.
axenic growth is reported to cause delayed development and poor growth  <cit> , and feeding animals e. coli in s basal shortens life span relative to growth on ngm agar  <cit> .
in addition, dr during development has been shown to be sub-optimal, since animals switched to dr just before adulthood are longer-lived than those maintained on dr from hatching  <cit> .
recently, two groups independently reported an novel reduced bacterial feeding dr protocol carried out under standard conditions  <cit> .
by measuring adult life span as a function of e. coli food concentration, both studies determined that complete removal of bacterial food early in adulthood  optimally increases median and maximum life span.
bd differs from previous studies describing dr by axenic growth in the culture method  and the time of initiation  <cit> .
although bacterial food is completely absent during adulthood in the bd regimen, bd-treated animals do not suffer from malnutrition , thus meeting the commonly accepted definition for dr. life span extension from bd has been recently validated in multiple wild-derived c. elegans strains, as well as in a second closely related nematode species c. remanei  <cit> .
like mutation of eat- <dig> or axenic growth  <cit> , bd appears to modulate longevity by a mechanism distinct from reduced iis  <cit> .
while bd results in a more robust life span increase than mutation of eat- <dig>  eat- <dig> mutants subjected to bd are not longer-lived than wild type animals on bd  <cit> .
taken together, these observations indicate that bd and mutation of eat- <dig> are likely to increase life span by similar or overlapping mechanisms.
thus, bd represents a simple plate-based method for dr that maximizes longevity from food restriction under standard nematode growth conditions and does not require additional genetic manipulation.
in order to better characterize the mechanism by which bd slows aging in c. elegans, we determined the effect of bd on survival as a function of the age at which bd is initiated.
previously, our group and an independent study  <cit>  reported that bd increases life span in c. elegans when initiated at the last stage of larval development  through day  <dig> of adulthood.
here, we expand this analysis to more advanced ages, after which mortality has become significant in the population.
similar to prior observations in drosophila  <cit>  and in mice  <cit> , we find that bd enhances survival even when imposed late in life.
transient exposure to bd confers a life span benefit that correlates with stress resistance, as measured by thermotolerance.
we further demonstrate that age-independent life span extension in response to bd is not solely due to reduced caloric intake, but is also influenced by a diffusible, cell-free component of the bacterial diet.
RESULTS
to determine the relationship between bd and age, animals were switched from control fed to bd diets, or vice versa, at different ages, and survival was monitored.
a significant increase in survival was observed when animals were transferred to bd at day  <dig>   <dig>   <dig>   <dig>  or  <dig> of adulthood .
this was true even when bd was initiated after more than 50% of the control-fed population had died.
animals placed on bd at the 2nd day of adulthood and returned to a control-fed diet at the 8th, 14th, or 28th day of adulthood lived longer than animals fed a control diet for their entire life .
by considering the survival of only those animals alive at the time of return to control fed conditions, it was apparent that transient bd induced a significant long-term protective effect resulting in enhanced survival after return to a control fed diet .
this is demonstrated by the significant increase in survival following return to a control fed diet, relative to animals maintained on a control fed diet for life.
'mean survival after transfer' is the average life span of animals in each group that were alive at the time the cohort was switched from control fed  to bacterial deprivation .
'mean control fed survival after transfer' is the average life span of control fed animals that were alive at the time the corresponding cohort was switched to bd.
survival data are shown as mean ± standard error.
p-values were calculated by the wilcoxon rank-sum test using the matlab 'ranksum' function.
'mean survival after transfer' is the average life span of animals in each group that were alive at the time the cohort was switched from bd back to a control fed  diet.
'mean control fed survival after transfer' is the average life span of control fed animals that were alive at the time the corresponding cohort was switched to bd.
survival data are shown as mean ± standard error.
p-values were calculated by the wilcoxon rank-sum test using the matlab 'ranksum' function.
bacterial deprivation  was initiated at the 2nd day of adulthood.
we have previously reported that bd induces a robust increase in thermotolerance  <cit> .
we wanted to determine whether worms exposed to transient bd retain this stress resistant phenotype, in correlation with the life span benefit.
we observed an age-dependent decrease in thermotolerance of both control fed and bd animals; however, bd significantly increased thermotolerance at every age-point examined , consistent with prior observations  <cit> .
interestingly, enhanced thermotolerance was also maintained in animals subjected to transient bd  , demonstrating that transient bd provides a long-term enhancement of thermotolerance as well as survival.
during our analysis of bd and the age at which it is initiated, we noted that older control fed animals qualitatively consumed less food than young animals, based on the rate at which bacterial food disappeared from the surface of the agar plates.
this is consistent with prior reports that the rate of pharyngeal pumping, which is necessary for food to enter the intestine, decreases dramatically with age  <cit> .
in a longitudinal study of age-related phenotypes, it was shown that no significant pumping can be measured in the last quartile of the mean life span  <cit> .
this suggested to us the possibility that bd might increase life span when initiated in animals that were no longer consuming a substantial amount of bacterial food.
to assay feeding behavior as a function of age, we quantified both food consumption and defecation.
food consumption was measured by mixing fluorescent beads comparable in size to bacterial cells with the e. coli food source and observing the presence of food within the gut by fluorescence microscopy.
within one hour of exposure, beads were observed in the intestine of 100% of young adult control-fed animals .
the percent of control-fed animals that are consuming beads decreased dramatically with age, however, and by  <dig> days of age, beads could not detected in the gut lumen of any animals assayed .
defecation rate also decreased as a function of age and defecation cycles were not observed in control-fed animals older than  <dig> days .
thus, as suggested by prior reports measuring pharyngeal pumping  <cit> , we conclude that consumption of bacterial cells by the control fed population decreases over the first 2– <dig> weeks of life and largely ceases by 20– <dig> days of adulthood.
since bd extends life span in animals no longer consuming bacteria , we considered the possibility that the bacterial food source produces a longevity-limiting signal that is sensed by the worms.
prior studies demonstrated that some sensory-defective c. elegans mutants have increased life span  <cit> .
the possibility that dietary cues directly mediate longevity was not examined in these studies, however, and based on physiological assays it was concluded that the long-lived chemosensory mutants were not mimics of dr  <cit> .
consistent with this conclusion and a recent study using a liquid bacterial dilution dr protocol  <cit> , we observed that bd significantly increased the life span of five long-lived chemosensory mutants examined by kenyon and colleagues  <cit> : che- <dig>  che- <dig>  daf- <dig>  osm- <dig>  and tax- <dig> .
three of these mutants , osm- <dig>  and tax-4) were previously demonstrated to be at least partially dependent on daf- <dig>  <cit> ; whereas life span extension by bd is daf- <dig> independent  <cit> .
bd significantly extends life span in each of these chemosensory mutants compared to control fed animals .
since the magnitude by which bd increased life span in long-lived chemosensory mutants was comparable to the percent life span increase in n <dig> animals, we suggest that life span extension from bd is likely to be mediated by a food sensing pathway that is at least partially distinct from the chemosensory mutants examined here.
life span was measured for a subset of chemosensory mutants, compared to n <dig>  on plates with food  or on plates without food .
the number of animals , mean survival , median survival, percent extension of the median, and p-values are given for each strain and condition.
data shown here are pooled from a minimum of two independent trials.
p-values were calculated by the wilcoxon rank-sum test using the matlab 'ranksum' function.
pair-wise comparisons testing for significant differences in median life span were made for each of the experimental conditions shown in table  <dig> and figure  <dig>  p-values were calculated by the wilcoxon rank-sum test using the matlab 'ranksum' function.
to test the hypothesis that decreased sensing of a dietary component underlies a portion of the life span extension from bd, we asked whether cell-free supernatant from an overnight culture of e. coli op <dig> would influence longevity.
addition of e. coli op <dig> conditioned supernatant significantly decreased the life span of bd animals .
this suppression of bd-induced longevity was not observed when fresh culture media was added to the diet, demonstrating that bacterial metabolism is required for production of the longevity-limiting signal.
life span was measured for n <dig> on plates with food , plates without food , bd plates treated with lb  or cell-free supernatant from an e. coli op <dig> culture , or plates containing a lawn of e. coli op <dig> beneath the surface of the agar .
the number of animals , mean survival , median survival, percent extension of the median, and p-values are given for each strain and condition.
data shown here are pooled from two independent trials.
p-values were calculated by the wilcoxon rank-sum test using the matlab 'ranksum' function.
pair-wise comparisons to test for significant differences between median life span were made for each experimental condition and p-values are given for each pair.
as a further test of our hypothesis, we determined the longevity of animals that were physically separated from the bacterial food by an agar barrier .
animals were grown on ngm + op <dig> until the 2nd day of adulthood, then transferred to the surface of either control fed, bd, or agar barrier plates.
similar to the effect of adding e. coli op <dig> conditioned supernatant to the diet, animals maintained on agar barrier plates had a life span that was intermediate between control fed and bd .
thus, we conclude that a diffusible component of the bacterial diet limits longevity, and the absence of this dietary signal accounts for a portion of the life span extension associated with bd.
CONCLUSIONS
the health and longevity benefits of dr initiated early in life are well established in a variety of organisms  <cit> .
studies in flies and rodents have indicated that at least some of these benefits can still be attained if dr is initiated during middle age  <cit> .
our work further supports this idea by demonstrating that bd significantly increases survival in c. elegans at any point during adulthood, at least up to the median population life span.
importantly, transient bd early in life results in a long-term protective effect, increasing both survival and stress resistance even after return to a control diet.
finally, we report the surprising observation that bd increases survival by a mechanism that is at least partially distinct from reduced food consumption, and involves reduced sensing of a diffusible signal produced by the bacterial component of the diet.
based on these observations, we propose that dr increases life span through two distinct mechanisms: calorie restriction and reduced food sensing .
the idea that dietary cues could influence nematode longevity via sensory signaling is supported by prior observations.
c. elegans is attracted to both soluble and volatile compounds produced by the bacterial food source, and animals can sense and respond to food through chemotaxis  <cit> .
although muscle tissue undergoes age-related deterioration, the nervous system remains remarkably intact in worms of advanced age,  <cit> .
this is consistent with our model that bd extends life span in worms of advanced age, not because of reduced food consumption, but because of the loss of a life span-limiting signal produced by the bacteria and sensed by the worms.
in addition, several mutants defective in chemosensation are long-lived.
the additive life span extension from combining long-lived chemosensory mutants with bd is consistent with our model, as these mutants were previously shown not to be genetic mimics of dr  <cit> .
indeed, since life span extension in chemosensory mutants is largely dependent on daf- <dig>  <cit> , and multiple epistasis studies have placed dr and daf- <dig> in different genetic pathways, an additive life span extension is the predicted outcome of these experiments.
how can a sensory mechanism of longevity determination by bd be reconciled with other models of dr in c. elegans?
mutation of eat- <dig> causes a pharyngeal pumping defect that results in reduced food consumption  <cit> , but has not been shown to cause sensory defects.
thus, the life span extension from mutation of eat- <dig> is likely due to a reduction in caloric intake.
interestingly, bd increases life span to a greater extent than mutation of eat- <dig>  and eat- <dig> mutants subjected to bd are not longer-lived than n <dig> animals subjected to bd  <cit> .
one interpretation of these data is that reduced food intake  is sufficient to increase life span, while reduced food intake coupled with reduced food sensing  leads to an even greater life span extension.
axenic culture is somewhat more complicated because, although the animals are maintained in the absence of food, as they are in bd, the growth media is very nutrient rich  and contains additional signals that may be sensed by c. elegans.
eat- <dig> mutants subjected to axenic culture live longer than n <dig> animals on axenic growth  <cit> , suggesting that mutation of eat- <dig> and axenic growth are not equivalent methods of dr.
previous studies have demonstrated a role for the bacterial food source in life span determination – through bacterial pathogenesis.
a number of bacterial strains have been shown to be pathogenic in c. elegans  <cit> , and worms cultured on a non-pathogenic species, b. subtilis, were shown to have extended longevity compared to those cultured on the normal food source, e. coli op <dig>  <cit> .
op <dig> has also been shown to affect life span through bacterial proliferation in the gut  <cit> .
in our studies, we maintain worms on uv-irradiated op <dig> as a food source to eliminate bacterial pathogenesis as a complicating factor in life span analysis.
however, we do find that a soluble product made by op <dig> partially suppresses the life span extension by bd, independent of bacterial proliferation, supporting our model that reduced food sensing contributes to the longevity observed in animals subjected to bd.
formally, it is possible that the bacteria produce a toxin that is life span-limiting, independent of the pathogenic effects of bacterial proliferation in the gut.
this does not change the interpretation of our data, however, which is that a diffusible, cell-free component of the diet can limit the life span extension from bd.
recent studies in the fruit fly have indicated that a volatile component of the diet can also suppress life span extension from dr in that organism  <cit> .
thus, modulation of longevity through food sensing may represent an evolutionarily conserved mechanism linking diet and aging.
it will be of interest to determine which are the precise chemical cues in the diet that influence longevity in each of these organisms.
at this time, there is little direct evidence that food sensing modulates aging in mammals; however, it is reasonable to speculate that food could induce relevant physiological changes through olfactory or taste cues, independent of food consumption.
this raises the intriguing possibility that one class of dr mimetics may be achieved by interfering with food sensing, but without requiring reduced caloric intake.

 dietary restriction, also referred to as calorie restriction, is an intervention that extends life span and delays the onset of age-related phenotypes in nearly all eukaryotic organisms in which it has been tested. simplistically, it is defined as a significant reduction in dietary intake in the absence of malnutrition. many different approaches can be used to achieve dr. in mice and rats for example, life span extension is observed by either reducing the amount of food consumed daily or by imposing an intermittent fasting regimen. in addition to simply reducing the amount of food intake, the effects of altering dietary composition has also been examined in different organisms. methionine-restricted mice and rats have an extended life span, suggesting that the nutritional composition of the diet can influence longevity in mammals. in fruit flies, reducing either the yeast extract or sugar composition of the food supply extends life span, although yeast extract appears to have the greatest impact on longevity. restriction of amino acids also increases life span in flies. in yeast, reducing either the amount of glucose or amino acids in the growth media increases replicative life span. multiple methods for dr have been used in c. elegans, as in other model organisms. one commonly used method in c. elegans is a genetic model, mutation of eat- the eat mutants were originally identified in a screen for defects in feeding behavior – they have reduced food consumption due to pharyngeal pumping defects. several alleles of eat- increase life span, with longevity generally correlating with the degree to which pumping rate is decreased. life span extension from mutation of eat- is independent of the foxo-family transcription factor daf- and is additive with a longevity-enhancing allele of the insulin/igf-1-like receptor daf-. this has led to the generally accepted model that dr acts to modulate longevity in a genetic pathway distinct from insulin/igf-1-like signaling. as with any genetic model of dr, however, eat mutants are not suitable for certain studies. the relative activity associated with different eat- alleles has not been completely characterized, so it is unclear whether life span extension from dr is maximized in studies using these alleles. in addition, eat mutants are food restricted from hatching, and dr during development may have secondary effects on adult physiology that are not fully understood. dr by reducing food availability also increases life span in c. elegans. the standard method for culturing c. elegans in the laboratory is to maintain the nematodes on the surface of nematode growth medium nutrient agar with e. coli op as the food source. dr on ngm nutrient agar has been achieved by reducing the amount of peptone in the media so as to limit bacterial growth or by reducing the amount of live bacteria present on the surface of the ngm nutrient agar. two methods of dr have also been described using non-standard, liquid-based growth conditions: bacterial dilution in s basal medium and axenic growth. both liquid-based methods increase life span relative to animals fed a diet of e. coli op in s basal, and axenic growth behaves similarly to mutation of eat- in epistasis experiments with iis. nonetheless, neither method is widely used for aging studies. this may be due to the potential differences in growth under standard conditions on an agar surface versus growth in liquid culture. axenic growth is reported to cause delayed development and poor growth, and feeding animals e. coli in s basal shortens life span relative to growth on ngm agar. in addition, dr during development has been shown to be sub-optimal, since animals switched to dr just before adulthood are longer-lived than those maintained on dr from hatching. recently, two groups independently reported an novel reduced bacterial feeding dr protocol carried out under standard conditions. by measuring adult life span as a function of e. coli food concentration, both studies determined that complete removal of bacterial food early in adulthood optimally increases median and maximum life span. bd differs from previous studies describing dr by axenic growth in the culture method and the time of initiation. although bacterial food is completely absent during adulthood in the bd regimen, bd-treated animals do not suffer from malnutrition, thus meeting the commonly accepted definition for dr. life span extension from bd has been recently validated in multiple wild-derived c. elegans strains, as well as in a second closely related nematode species c. remanei. like mutation of eat- or axenic growth, bd appears to modulate longevity by a mechanism distinct from reduced iis. while bd results in a more robust life span increase than mutation of eat- eat- mutants subjected to bd are not longer-lived than wild type animals on bd. taken together, these observations indicate that bd and mutation of eat- are likely to increase life span by similar or overlapping mechanisms. thus, bd represents a simple plate-based method for dr that maximizes longevity from food restriction under standard nematode growth conditions and does not require additional genetic manipulation. in order to better characterize the mechanism by which bd slows aging in c. elegans, we determined the effect of bd on survival as a function of the age at which bd is initiated. previously, our group and an independent study reported that bd increases life span in c. elegans when initiated at the last stage of larval development through day of adulthood. here, we expand this analysis to more advanced ages, after which mortality has become significant in the population. similar to prior observations in drosophila and in mice, we find that bd enhances survival even when imposed late in life. transient exposure to bd confers a life span benefit that correlates with stress resistance, as measured by thermotolerance. we further demonstrate that age-independent life span extension in response to bd is not solely due to reduced caloric intake, but is also influenced by a diffusible, cell-free component of the bacterial diet.  to determine the relationship between bd and age, animals were switched from control fed to bd diets, or vice versa, at different ages, and survival was monitored. a significant increase in survival was observed when animals were transferred to bd at day or of adulthood. this was true even when bd was initiated after more than 50% of the control-fed population had died. animals placed on bd at the 2nd day of adulthood and returned to a control-fed diet at the 8th, 14th, or 28th day of adulthood lived longer than animals fed a control diet for their entire life. by considering the survival of only those animals alive at the time of return to control fed conditions, it was apparent that transient bd induced a significant long-term protective effect resulting in enhanced survival after return to a control fed diet. this is demonstrated by the significant increase in survival following return to a control fed diet, relative to animals maintained on a control fed diet for life.'mean survival after transfer' is the average life span of animals in each group that were alive at the time the cohort was switched from control fed to bacterial deprivation.'mean control fed survival after transfer' is the average life span of control fed animals that were alive at the time the corresponding cohort was switched to bd. survival data are shown as mean ± standard error. p-values were calculated by the wilcoxon rank-sum test using the matlab'ranksum' function.'mean survival after transfer' is the average life span of animals in each group that were alive at the time the cohort was switched from bd back to a control fed diet.'mean control fed survival after transfer' is the average life span of control fed animals that were alive at the time the corresponding cohort was switched to bd. survival data are shown as mean ± standard error. p-values were calculated by the wilcoxon rank-sum test using the matlab'ranksum' function. bacterial deprivation was initiated at the 2nd day of adulthood. we have previously reported that bd induces a robust increase in thermotolerance. we wanted to determine whether worms exposed to transient bd retain this stress resistant phenotype, in correlation with the life span benefit. we observed an age-dependent decrease in thermotolerance of both control fed and bd animals; however, bd significantly increased thermotolerance at every age-point examined, consistent with prior observations. interestingly, enhanced thermotolerance was also maintained in animals subjected to transient bd, demonstrating that transient bd provides a long-term enhancement of thermotolerance as well as survival. during our analysis of bd and the age at which it is initiated, we noted that older control fed animals qualitatively consumed less food than young animals, based on the rate at which bacterial food disappeared from the surface of the agar plates. this is consistent with prior reports that the rate of pharyngeal pumping, which is necessary for food to enter the intestine, decreases dramatically with age. in a longitudinal study of age-related phenotypes, it was shown that no significant pumping can be measured in the last quartile of the mean life span. this suggested to us the possibility that bd might increase life span when initiated in animals that were no longer consuming a substantial amount of bacterial food. to assay feeding behavior as a function of age, we quantified both food consumption and defecation. food consumption was measured by mixing fluorescent beads comparable in size to bacterial cells with the e. coli food source and observing the presence of food within the gut by fluorescence microscopy. within one hour of exposure, beads were observed in the intestine of 100% of young adult control-fed animals. the percent of control-fed animals that are consuming beads decreased dramatically with age, however, and by days of age, beads could not detected in the gut lumen of any animals assayed. defecation rate also decreased as a function of age and defecation cycles were not observed in control-fed animals older than days. thus, as suggested by prior reports measuring pharyngeal pumping, we conclude that consumption of bacterial cells by the control fed population decreases over the first 2– weeks of life and largely ceases by 20– days of adulthood. since bd extends life span in animals no longer consuming bacteria, we considered the possibility that the bacterial food source produces a longevity-limiting signal that is sensed by the worms. prior studies demonstrated that some sensory-defective c. elegans mutants have increased life span. the possibility that dietary cues directly mediate longevity was not examined in these studies, however, and based on physiological assays it was concluded that the long-lived chemosensory mutants were not mimics of dr. consistent with this conclusion and a recent study using a liquid bacterial dilution dr protocol, we observed that bd significantly increased the life span of five long-lived chemosensory mutants examined by kenyon and colleagues: che- che- daf- osm- and tax-. three of these mutants, osm- and tax-4) were previously demonstrated to be at least partially dependent on daf-; whereas life span extension by bd is daf- independent. bd significantly extends life span in each of these chemosensory mutants compared to control fed animals. since the magnitude by which bd increased life span in long-lived chemosensory mutants was comparable to the percent life span increase in n animals, we suggest that life span extension from bd is likely to be mediated by a food sensing pathway that is at least partially distinct from the chemosensory mutants examined here. life span was measured for a subset of chemosensory mutants, compared to n on plates with food or on plates without food. the number of animals, mean survival, median survival, percent extension of the median, and p-values are given for each strain and condition. data shown here are pooled from a minimum of two independent trials. p-values were calculated by the wilcoxon rank-sum test using the matlab'ranksum' function. pair-wise comparisons testing for significant differences in median life span were made for each of the experimental conditions shown in table and figure p-values were calculated by the wilcoxon rank-sum test using the matlab'ranksum' function. to test the hypothesis that decreased sensing of a dietary component underlies a portion of the life span extension from bd, we asked whether cell-free supernatant from an overnight culture of e. coli op would influence longevity. addition of e. coli op conditioned supernatant significantly decreased the life span of bd animals. this suppression of bd-induced longevity was not observed when fresh culture media was added to the diet, demonstrating that bacterial metabolism is required for production of the longevity-limiting signal. life span was measured for n on plates with food, plates without food, bd plates treated with lb or cell-free supernatant from an e. coli op culture, or plates containing a lawn of e. coli op beneath the surface of the agar. the number of animals, mean survival, median survival, percent extension of the median, and p-values are given for each strain and condition. data shown here are pooled from two independent trials. p-values were calculated by the wilcoxon rank-sum test using the matlab'ranksum' function. pair-wise comparisons to test for significant differences between median life span were made for each experimental condition and p-values are given for each pair. as a further test of our hypothesis, we determined the longevity of animals that were physically separated from the bacterial food by an agar barrier. animals were grown on ngm + op until the 2nd day of adulthood, then transferred to the surface of either control fed, bd, or agar barrier plates. similar to the effect of adding e. coli op conditioned supernatant to the diet, animals maintained on agar barrier plates had a life span that was intermediate between control fed and bd. thus, we conclude that a diffusible component of the bacterial diet limits longevity, and the absence of this dietary signal accounts for a portion of the life span extension associated with bd.  the health and longevity benefits of dr initiated early in life are well established in a variety of organisms. studies in flies and rodents have indicated that at least some of these benefits can still be attained if dr is initiated during middle age. our work further supports this idea by demonstrating that bd significantly increases survival in c. elegans at any point during adulthood, at least up to the median population life span. importantly, transient bd early in life results in a long-term protective effect, increasing both survival and stress resistance even after return to a control diet. finally, we report the surprising observation that bd increases survival by a mechanism that is at least partially distinct from reduced food consumption, and involves reduced sensing of a diffusible signal produced by the bacterial component of the diet. based on these observations, we propose that dr increases life span through two distinct mechanisms: calorie restriction and reduced food sensing. the idea that dietary cues could influence nematode longevity via sensory signaling is supported by prior observations. c. elegans is attracted to both soluble and volatile compounds produced by the bacterial food source, and animals can sense and respond to food through chemotaxis. although muscle tissue undergoes age-related deterioration, the nervous system remains remarkably intact in worms of advanced age,. this is consistent with our model that bd extends life span in worms of advanced age, not because of reduced food consumption, but because of the loss of a life span-limiting signal produced by the bacteria and sensed by the worms. in addition, several mutants defective in chemosensation are long-lived. the additive life span extension from combining long-lived chemosensory mutants with bd is consistent with our model, as these mutants were previously shown not to be genetic mimics of dr. indeed, since life span extension in chemosensory mutants is largely dependent on daf-, and multiple epistasis studies have placed dr and daf- in different genetic pathways, an additive life span extension is the predicted outcome of these experiments. how can a sensory mechanism of longevity determination by bd be reconciled with other models of dr in c. elegans? mutation of eat- causes a pharyngeal pumping defect that results in reduced food consumption, but has not been shown to cause sensory defects. thus, the life span extension from mutation of eat- is likely due to a reduction in caloric intake. interestingly, bd increases life span to a greater extent than mutation of eat- and eat- mutants subjected to bd are not longer-lived than n animals subjected to bd. one interpretation of these data is that reduced food intake is sufficient to increase life span, while reduced food intake coupled with reduced food sensing leads to an even greater life span extension. axenic culture is somewhat more complicated because, although the animals are maintained in the absence of food, as they are in bd, the growth media is very nutrient rich and contains additional signals that may be sensed by c. elegans. eat- mutants subjected to axenic culture live longer than n animals on axenic growth, suggesting that mutation of eat- and axenic growth are not equivalent methods of dr. previous studies have demonstrated a role for the bacterial food source in life span determination – through bacterial pathogenesis. a number of bacterial strains have been shown to be pathogenic in c. elegans, and worms cultured on a non-pathogenic species, b. subtilis, were shown to have extended longevity compared to those cultured on the normal food source, e. coli op. op has also been shown to affect life span through bacterial proliferation in the gut. in our studies, we maintain worms on uv-irradiated op as a food source to eliminate bacterial pathogenesis as a complicating factor in life span analysis. however, we do find that a soluble product made by op partially suppresses the life span extension by bd, independent of bacterial proliferation, supporting our model that reduced food sensing contributes to the longevity observed in animals subjected to bd. formally, it is possible that the bacteria produce a toxin that is life span-limiting, independent of the pathogenic effects of bacterial proliferation in the gut. this does not change the interpretation of our data, however, which is that a diffusible, cell-free component of the diet can limit the life span extension from bd. recent studies in the fruit fly have indicated that a volatile component of the diet can also suppress life span extension from dr in that organism. thus, modulation of longevity through food sensing may represent an evolutionarily conserved mechanism linking diet and aging. it will be of interest to determine which are the precise chemical cues in the diet that influence longevity in each of these organisms. at this time, there is little direct evidence that food sensing modulates aging in mammals; however, it is reasonable to speculate that food could induce relevant physiological changes through olfactory or taste cues, independent of food consumption. this raises the intriguing possibility that one class of dr mimetics may be achieved by interfering with food sensing, but without requiring reduced caloric intake. 
these survival benefits are, at least partially, independent of food consumption, as control fed animals are no longer consuming bacterial food at this advanced age. based on these findings, we propose that dietary restriction by bacterial deprivation increases longevity in c. elegans by a combination of reduced food consumption and decreased food sensing. dietary restriction increases life span and delays age-associated disease in many organisms. thus, we find that life span extension from bacterial deprivation can be partially suppressed by a diffusible component of the bacterial food source, suggesting a calorie-independent mechanism for life span extension by dietary restriction. using bacterial food deprivation as a means of dr in c. elegans, we show that transient dr confers long-term benefits including stress resistance and increased longevity. dr by bacterial food deprivation significantly increases life span in worms when initiated as late as days of adulthood, an age at which greater than 50% of the cohort have died. animals separated from the bacterial lawn by a barrier of solid agar have a life span intermediate between control fed and food restricted animals. consistent with studies in the fruit fly and in mice, we demonstrate that dr also enhances survival when initiated late in life.
 dietary restriction increases life span and delays age-associated disease in many organisms. the mechanism by which dr enhances longevity is not well understood.  using bacterial food deprivation as a means of dr in c. elegans, we show that transient dr confers long-term benefits including stress resistance and increased longevity. consistent with studies in the fruit fly and in mice, we demonstrate that dr also enhances survival when initiated late in life. dr by bacterial food deprivation significantly increases life span in worms when initiated as late as days of adulthood, an age at which greater than 50% of the cohort have died. these survival benefits are, at least partially, independent of food consumption, as control fed animals are no longer consuming bacterial food at this advanced age. animals separated from the bacterial lawn by a barrier of solid agar have a life span intermediate between control fed and food restricted animals. thus, we find that life span extension from bacterial deprivation can be partially suppressed by a diffusible component of the bacterial food source, suggesting a calorie-independent mechanism for life span extension by dietary restriction.  based on these findings, we propose that dietary restriction by bacterial deprivation increases longevity in c. elegans by a combination of reduced food consumption and decreased food sensing. 