BACKGROUND
transposable elements  are repetitive sequences found in nearly every eukaryotic species and contribute to a large proportion of the genome in many species.
accurate characterization and categorization of repetitive elements in a genome can be challenging, sometimes significant underestimated in early attempts even in sequenced genomes and previously undiscovered elements continue to emerge.
for example, the drosophila melanogaster genome was thought to contain 6-8% tes, it was soon revised to ~22%  <cit> .
human genome was believed to contain about ~50% repeats, a recent study suggested that they may contribute up to two thirds of the genome  <cit> .
in addition to the fundamental knowledge brought about by research on tes, the disruptive forces of tes can be harnessed and utilized to benefit scientific research and technology development.
these te derived tools fall into different application categories.
tes can be used to create stable transgenic organisms that are essential for studies on insertional mutagenesis, enhancer trapping and gene trapping  <cit> .
due to their disruptive nature, tes are capable of inserting into multiple sites of a gene, creating a series of te tagged mutation lines  <cit> .
different insertional mutant lines may have different levels of severity in terms of phenotypic changes.
dna-type elements are unstable and their excision from an insertional mutant line may restore expression of the disrupted genes, resulting in reversion from mutant phenotypes.
these revertant lines provide support for the causative genotype for a phenotype  <cit> .
the target genes in te tagged lines can be cloned based on the te tag sequences, a major advantage over the random point mutagenesis approaches .
development of this technology for a wide range of hosts requires deep understanding of transposition mechanisms of a variety of elements.
the abundance of tes in most large genomes also make them candidates as genetic markers  <cit> .
gene therapy to treat genetic diseases and cancers using viral vectors encountered significant challenges due to immune response to the viral vectors  <cit> , leading researchers to consider dna transposons for alternative vectors  <cit> .
it has also been demonstrated that piggybac transposons can be used to create marker free induced pluripotency stem cells  <cit> .
genetic control of pest insect population traditionally uses radiation for mass production of sterile male insects.
with the insect transformation technology to create transgenic insects bearing genes that can be used for their control, transposons gained much attention to be potential gene drive vectors to spread transgenes among pest populations to achieve genetic control  <cit> .
yellow fever mosquito, aedes aegypti, is a vector for yellow fever, dengue and chikungunia virus.
large scale mosquito control has been proven a challenging task.
recently, using biotechnology in mosquito control such as oxitec has garnered much attention and is currently under pilot test  <cit> .
in this approach, a large number of transgenic male mosquitoes are released to outcompete wild male mosquitoes to mate with female mosquitoes.
the transgene causes larva lethality in the next generation.
to achieve continuous control effect, a massive number of mosquitoes need to be released on a regular basis.
in addition, the ecological consequences, due to the changed mosquito population size and structure, remain a concern.
therefore, cost efficient approaches that introduce minimal alteration of the ecosystem are desirable.
one attractive approach is population replacement where a small number of transgenic mosquitoes resistant to pathogens can spread the transgenes to the wild population, pushing the defense line beyond the disease vectors.
a critical factor in this approach relies on a gene drive system to spread transgenes.
transposons can increase their frequency in a population, therefore are considered good candidates for a gene drive system  <cit> .
transgenic mosquitoes resistant to pathogens such as dengue virus and plasmodium falciparum have been established  <cit> .
however, the commonly used transposons for insect transgenesis rarely remobilize efficiently once they integrate into the mosquito chromosomes  <cit> .
therefore, endogenous active transposon elements in the mosquito genome may provide a much needed tool.
tes comprise 47% of the genome of aedes aegypti, a rich source of materials to search for active tes.
tefam, a database dedicated for vector insects, contains a total of  <dig> a. aetypti te families,  <dig> characterized as retro element families and  <dig> dna element families.
many of these families are non-autonomous elements that depend on their autonomous partners to provide transposase for their mobilization.
for example there are  <dig> mite families that account for about 16% of the total genome sequences  <cit> .
the availability of these te sequences provides an opportunity to identify candidates for active endogenous tes in the yellow fever mosquito genome.
previous reverse genetic approaches for active te discovery suggested that the presence of multiple identical copies of an element is a flashing indicator for current or very recent transposition activity  <cit> .
in this study, genome wide analyses were performed to identify elements with multiple identical copies inserted at difference loci.
further analysis of the best candidates for active transposons led to the identification and characterization of  <dig> families  of novel transposable elements designated as atons.
these elements are non-autonomous bearing terminal inverted repeats and insert specifically at dinucleotide “at”, a feature not seen in known te superfamilies.
the autonomous elements for atons remain mysterious despite exhaustive genome wide database searches.
RESULTS
identification of aton-i and aton-ii families in a. aegypti
the genome of a. aegypti has a high te content particularly rich in mites  <cit> .
in an effort to understand the evolution and activity of tes in this genome, searches for elements that have multiple identical copies located in different flanking sequences were carried out.
all elements that bear complete ends, referred as complete elements, from the  <dig> te families in tefam were retrieved using mak  <cit> .
the sequences in each family were used to identify identical copies.
out of the  <dig> te families,  <dig> sequences from  <dig> families have at least five identical copies .
a total of  <dig> sequences from  <dig> families have at least  <dig> identical copies in the genome .
eight of the  <dig> sequences are retro elements from three families.
among these, the tsine family tf <dig> is particularly rich in identical sequences with four having more than  <dig> identical copies:  <dig>   <dig>   <dig> and  <dig>  out of the total copy number of  <dig>  sixteen of the  <dig> sequences are dna elements from nine families.
three dna te families, tf <dig>  tf <dig> and tf <dig>  are particularly rich in identical sequences.
tf <dig> has one sequence with  <dig> identical copies out of  <dig>  tf <dig> has three sequences with identical copies of  <dig>   <dig> and  <dig>  tf <dig> has three sequences with identical copies of  <dig>   <dig> and  <dig> .
all of these three families were categorized in tefam as mites with a target site duplication sequence of “ta” .
* the sequences for these entries can be found in the additional file 4: sequence file.
for further analyses, the complete copies for each of the three mite families were retrieved with their flanking sequences.
when the sequences from each family were aligned, the target site duplication of dinucleotide “ta” for tf <dig> was confirmed, but not for tf <dig> and tf <dig>  in these two families, the sequence conservation extended to an additional nucleotide on each end: the 5’ an “a” and the 3’ end a “t”  .
tes can occasionally insert into other repetitive sequences.
to identify the junction between the elements in tf <dig> and tf <dig> families and their flanking sequences, if present, the related empty sequences  of the flanking sequences were retrieved and aligned.
it was found that a dinucleotide “at” was present in the te insertion site on the ress .
however, whether the dinucleotide “at” is duplicated upon insertion or an element inserts between “a” and “t” is not clear.
the dinucleotide “ta”, which is observed in the ress for stowaway-like mites, was not observed in the ress.
the specific integration of the tes bearing terminal inverted repeats at dinucleotide “at” is a novel feature, therefore they were designated as aton for “at” specific insertion transposons.
the repeat families represented by tf <dig> and tf <dig> were subsequently termed aton-i and aton-ii.
features of aton-i and aton-ii
aton-i and ii elements are  <dig> and 144 bp respectively.
conceptual folding of these aton elements revealed that they bear tirs of  <dig> and 17 bp .
while the terminal sequence of “ataggcc” is conserved between aton-i and ii, the internal sequences of these two families do not share significant similarity.
although a putative stem-loop structure is present in the subterminal regions on both 5’ and 3’ ends of aton-i, no such structure is apparent in the subterminal regions of aton-ii.
within the sequenced a. aegypti genome, there are  <dig> and  <dig> complete copies of aton-i and ii respectively.
ress can be found for approximately half  of these elements.
despite the observation that the vast majority of “at” insertion target sites in the flanking sequences of aton elements were intact, aberrant empty sites that do not contain intact target sites were found from analyses of all the res sequences .
they can be grouped into the following four categories:  <cit>  completely missing the dinucleotide “at”;  <cit>  partially missing the dinucleotide “at”;  <cit>  deletion beyond the dinucleotide;  <cit>  presence of additional nucleotides that are not present on the flanking sequences of aton.
these imperfect res sequences are rare  among the total number of res sequences for the atons.
these aberrant empty sites are often the only mutations over a long stretch of flanking sequences , suggesting that they may have resulted from the transposition activity of atons.
recent expansion of aton-i and aton-ii families
the large copy number of an aton family allows the estimation of its proliferation history.
when a te family is amplified from a few original copies, the relative age of each element can be measured by the level of sequence divergence from the consensus sequence.
the sequence divergence for each aton element from its corresponding family consensus sequences was calculated.
the number of elements of a defined sequence divergence value was plotted against sequence divergence values to obtain relative proliferation time for each aton family .
for aton-i,  <dig> elements have identical sequences  compared to the consensus sequence of the family and  <dig> copies have divergence values between 0-1%.
only  <dig> copies exist at divergence values ranging from 2% up to 13%.
in aton-ii,  <dig> elements have sequences identical to the consensus sequence and  <dig> copies have a divergence value ≤ 1%.
forty seven copies exist at divergence values between 1% and 10%.
three copies exist at divergence values from 10% to 20%.
to understand the evolution of the aton families, phylogenetic analyses for the two aton families were performed.
the elements in aton-i family can be grouped into five subclades .
subclades “a” to “d” each contain a few elements.
in contrast, subclade “e” is large and contains many identical element copies.
similarly, the elements in aton-ii can be grouped into seven subclades .
subclades “a” to “g” each contain a few elements that show minimal sequence similarities.
subclade “i” contains  <dig> copies that demonstrated some relatedness to other members in the clade whereas subclade “h" contained the largest number of elements with several identical copies .
the phylogenetic analyses suggested that the high copy numbers for these elements are due to recent amplification of these elements a few ancient copies.
distribution of aton-i in different strains of a. aegypti
there can be differential accumulation of various te families in different strains or ecotypes of a species.
for example, different lines of the same rice cultivar contain dramatically different copy numbers, from  <dig> to ~ <dig>  of the active mite family mping <cit> .
since aton-i family appears to have amplified very recently in the sequenced liverpool strain of a. aegypti which was collected in  <dig> from west africa, it is interesting to know whether this aton family is similarly distributed in a. aegypti mosquito strains collected from other locations.
in the americas a. aegypti was first introduced from africa during european exploration and colonization in the late 1500 s thus providing at least four centuries of separation.
the distribution of aton-i family in mosquito strains collected from different locations in the americas was surveyed .
these strains included costa rica , ugal  and rockefeller .
khw is a white-eyed mutant line of rockefeller.
the copy numbers of aton-i in these strains of a. aegypti were estimated using transposon display, a modified version of amplified fragment length polymorphism  .
when one selective base  was used to reduce the total number of bands in each lane, approximately  <dig> bands were visible on each lane of the liverpool strain, consistent with the expected results .
despite the fact that the liverpool mosquitoes used in this study underwent at least  <dig> consecutive generations of single pair inbreeding, there are still about  <dig>  bands have different sizes between the two individuals.
a. aegypti has homomorphic sex chromosomes with a small sex-determining region on chromosome  <dig>  therefore the different sized bands are probably not caused solely by the different sex of the two individuals.
the other strains showed a similar number of bands to that of the liverpool strain and a similar level of polymorphism between two individuals.
these results suggest that aton-i has amplified to a similar copy number in all of the tested strains of a. aegypti.
abundant new aton families in a. aegypti genome
to find out how abundant atons are in the a. aegypti genome, exhaustive genome wide searches were performed to identify repetitive elements that contain terminal inverted repeats flanked by nucleotides “a” and “t”.
the ress, when exist, were retrieved to confirm the target site dinucleotide “at”.
a total of  <dig> aton-like families were identified, including the two families described above.
interestingly, all of these elements have the nucleotide “t” internal to the “a” on the 5’ end and an internal “a” to the “t” on the 3’ end .
the additional  <dig> aton families contributed to a total  <dig> copies.
sequences in each of the  <dig> families are more diverged than those in aton-i and aton-ii .
nevertheless, three families have an element with more than  <dig> identical copies: aton-xii with  <dig> out of  <dig>  aton-xxii with  <dig> out of  <dig> and xxiv with  <dig> out of  <dig>  suggesting that these elements were recently or may be currently active .
while each aton family has a unique internal sequence and tir sequence, weak sequence similarity exists at the extreme ends of tirs among some families .
based on this sequence similarity, five groups that account for  <dig> atons families can be identified.
the length of the similar regions ranges from  <dig> to  <dig> nucleotides.
to investigate whether atons are transcribed, ests containing aton sequences were retrieved from databases.
of the  <dig> families,  <dig> resulted in est hits.
the number of hit sequences varied from  <dig> to  <dig> among the families .
since atons do not encode proteins, their presence on ests in the untranslated regions resulted from the cotranscription with the genes  they are located in.
comparison of atons with helitrons and mites
atons lack protein coding capacity and their transposition is dependent on transposases produced by autonomous elements.
identification of putative transposase sources for atons will shed light on their transposition mechanisms and facilitate their classification.
the autonomous elements of dna tes are typically expected to bear similar tirs, to the non-autonomous elements they mobilize.
occasionally, these similarities can be very limited; consequently, identifying the autonomous partner is more challenging.
extensive searches for large dna fragments flanked by similar terminal sequences to those of atons were performed.
all of the genomic dna fragments from 500 bp to 200 kb flanked by the termini  of atons were retrieved and analyzed.
close inspection of these large fragments did not yield conclusive clues to the putative transposase for atons.
since aton elements, like helitrons, are always flanked by an “a” on the 5’ end and a “t” on the 3’ end, it is possible that atons are related to helitron elements that insert between “a” and “t”.
however, there are marked difference between atons and reported helitrons.
the latter have conserved terminal motifs of 5’ “tc” and 3’ “ctrr”, although atons have a “t” internal to the 5’ “a”, only  <dig> families have a “c” after it  <cit> .
helitrons were originally thought to bear subterminal palindromic sequences, a number of elements found in fungi, sea urchin, sea anemone and drosophila are often palindrome free  <cit> .
the presence of subterminal palindromic motifs on some atons resembles that on some helitrons.
helitrons typically do not bear tirs.
however, it has been reported that the maize genome contain heltir sequences that have terminal inverted repeats resembling helitron 3' termini  <cit> .
to see whether there are autonomous helitrons in a. aegyypti genome that bear the tir sequences of atons as a terminus, genomic sequences of 20 kb near aton tir sequences were analyzed, nevertheless no helitron protein domains  were found in these sequences.
on the other hand, atons share some similarity in features to mites, they are quite small  and have tirs.
as described in results, the dinucleotide “at” may also be considered as a specific tsd sequence.
if atons are mites, it is possible that transposase coding autonomous elements bearing the tir sequences of atons may exist in the genome.
in an effort to find such elements in the whole genome, genomic sequences  flanked by aton tirs were analyzed.
however, no conclusive evidence was found for the presence of such transposase coding elements.
a similar scenario occurred for the identity of a recently discovered drosophila element named dine-i  <cit> .
however, dine-i elements do not bear tirs that are the critical features of mites.
in addition, dine-i has a preferred insertion site of “tt” and partial rephel coding sequences were found to be associated with some elements.
these feature led to the proposal that dine-i elements are helitrons.
in contrast, atons bear tirs like mites and insert at “at” like typical helitrons.
therefore, the classification of atons and components of their transposition machineries remain to be determined.
CONCLUSIONS
genome wide analyses of tes in a. aegypti identified  <dig> candidate elements from  <dig> familes for very recent or current transposition activity.
among the best candidate tes are five aton families, novel elements bear terminal inverted repeats and insert specifically at dinucleotide “at”.
in this study,  <dig> previously unidentified te families with nearly  <dig> copies were characterized.
however, despite exhaustive search efforts in the genome sequences, the autonomous elements and the classification of atons remain mysterious.

 transposable elements are repetitive sequences found in nearly every eukaryotic species and contribute to a large proportion of the genome in many species. accurate characterization and categorization of repetitive elements in a genome can be challenging, sometimes significant underestimated in early attempts even in sequenced genomes and previously undiscovered elements continue to emerge. for example, the drosophila melanogaster genome was thought to contain 6-8% tes, it was soon revised to ~22%. human genome was believed to contain about ~50% repeats, a recent study suggested that they may contribute up to two thirds of the genome. in addition to the fundamental knowledge brought about by research on tes, the disruptive forces of tes can be harnessed and utilized to benefit scientific research and technology development. these te derived tools fall into different application categories. tes can be used to create stable transgenic organisms that are essential for studies on insertional mutagenesis, enhancer trapping and gene trapping. due to their disruptive nature, tes are capable of inserting into multiple sites of a gene, creating a series of te tagged mutation lines. different insertional mutant lines may have different levels of severity in terms of phenotypic changes. dna-type elements are unstable and their excision from an insertional mutant line may restore expression of the disrupted genes, resulting in reversion from mutant phenotypes. these revertant lines provide support for the causative genotype for a phenotype. the target genes in te tagged lines can be cloned based on the te tag sequences, a major advantage over the random point mutagenesis approaches. development of this technology for a wide range of hosts requires deep understanding of transposition mechanisms of a variety of elements. the abundance of tes in most large genomes also make them candidates as genetic markers. gene therapy to treat genetic diseases and cancers using viral vectors encountered significant challenges due to immune response to the viral vectors, leading researchers to consider dna transposons for alternative vectors. it has also been demonstrated that piggybac transposons can be used to create marker free induced pluripotency stem cells. genetic control of pest insect population traditionally uses radiation for mass production of sterile male insects. with the insect transformation technology to create transgenic insects bearing genes that can be used for their control, transposons gained much attention to be potential gene drive vectors to spread transgenes among pest populations to achieve genetic control. yellow fever mosquito, aedes aegypti, is a vector for yellow fever, dengue and chikungunia virus. large scale mosquito control has been proven a challenging task. recently, using biotechnology in mosquito control such as oxitec has garnered much attention and is currently under pilot test. in this approach, a large number of transgenic male mosquitoes are released to outcompete wild male mosquitoes to mate with female mosquitoes. the transgene causes larva lethality in the next generation. to achieve continuous control effect, a massive number of mosquitoes need to be released on a regular basis. in addition, the ecological consequences, due to the changed mosquito population size and structure, remain a concern. therefore, cost efficient approaches that introduce minimal alteration of the ecosystem are desirable. one attractive approach is population replacement where a small number of transgenic mosquitoes resistant to pathogens can spread the transgenes to the wild population, pushing the defense line beyond the disease vectors. a critical factor in this approach relies on a gene drive system to spread transgenes. transposons can increase their frequency in a population, therefore are considered good candidates for a gene drive system. transgenic mosquitoes resistant to pathogens such as dengue virus and plasmodium falciparum have been established. however, the commonly used transposons for insect transgenesis rarely remobilize efficiently once they integrate into the mosquito chromosomes. therefore, endogenous active transposon elements in the mosquito genome may provide a much needed tool. tes comprise 47% of the genome of aedes aegypti, a rich source of materials to search for active tes. tefam, a database dedicated for vector insects, contains a total of a. aetypti te families, characterized as retro element families and dna element families. many of these families are non-autonomous elements that depend on their autonomous partners to provide transposase for their mobilization. for example there are mite families that account for about 16% of the total genome sequences. the availability of these te sequences provides an opportunity to identify candidates for active endogenous tes in the yellow fever mosquito genome. previous reverse genetic approaches for active te discovery suggested that the presence of multiple identical copies of an element is a flashing indicator for current or very recent transposition activity. in this study, genome wide analyses were performed to identify elements with multiple identical copies inserted at difference loci. further analysis of the best candidates for active transposons led to the identification and characterization of families of novel transposable elements designated as atons. these elements are non-autonomous bearing terminal inverted repeats and insert specifically at dinucleotide “at”, a feature not seen in known te superfamilies. the autonomous elements for atons remain mysterious despite exhaustive genome wide database searches.  identification of aton-i and aton-ii families in a. aegypti the genome of a. aegypti has a high te content particularly rich in mites. in an effort to understand the evolution and activity of tes in this genome, searches for elements that have multiple identical copies located in different flanking sequences were carried out. all elements that bear complete ends, referred as complete elements, from the te families in tefam were retrieved using mak. the sequences in each family were used to identify identical copies. out of the te families, sequences from families have at least five identical copies. a total of sequences from families have at least identical copies in the genome. eight of the sequences are retro elements from three families. among these, the tsine family tf is particularly rich in identical sequences with four having more than identical copies: and out of the total copy number of sixteen of the sequences are dna elements from nine families. three dna te families, tf tf and tf are particularly rich in identical sequences. tf has one sequence with identical copies out of tf has three sequences with identical copies of and tf has three sequences with identical copies of and. all of these three families were categorized in tefam as mites with a target site duplication sequence of “ta”. * the sequences for these entries can be found in the additional file 4: sequence file. for further analyses, the complete copies for each of the three mite families were retrieved with their flanking sequences. when the sequences from each family were aligned, the target site duplication of dinucleotide “ta” for tf was confirmed, but not for tf and tf in these two families, the sequence conservation extended to an additional nucleotide on each end: the 5’ an “a” and the 3’ end a “t”. tes can occasionally insert into other repetitive sequences. to identify the junction between the elements in tf and tf families and their flanking sequences, if present, the related empty sequences of the flanking sequences were retrieved and aligned. it was found that a dinucleotide “at” was present in the te insertion site on the ress. however, whether the dinucleotide “at” is duplicated upon insertion or an element inserts between “a” and “t” is not clear. the dinucleotide “ta”, which is observed in the ress for stowaway-like mites, was not observed in the ress. the specific integration of the tes bearing terminal inverted repeats at dinucleotide “at” is a novel feature, therefore they were designated as aton for “at” specific insertion transposons. the repeat families represented by tf and tf were subsequently termed aton-i and aton-ii. features of aton-i and aton-ii aton-i and ii elements are and 144 bp respectively. conceptual folding of these aton elements revealed that they bear tirs of and 17 bp. while the terminal sequence of “ataggcc” is conserved between aton-i and ii, the internal sequences of these two families do not share significant similarity. although a putative stem-loop structure is present in the subterminal regions on both 5’ and 3’ ends of aton-i, no such structure is apparent in the subterminal regions of aton-ii. within the sequenced a. aegypti genome, there are and complete copies of aton-i and ii respectively. ress can be found for approximately half of these elements. despite the observation that the vast majority of “at” insertion target sites in the flanking sequences of aton elements were intact, aberrant empty sites that do not contain intact target sites were found from analyses of all the res sequences. they can be grouped into the following four categories: completely missing the dinucleotide “at”; partially missing the dinucleotide “at”; deletion beyond the dinucleotide; presence of additional nucleotides that are not present on the flanking sequences of aton. these imperfect res sequences are rare among the total number of res sequences for the atons. these aberrant empty sites are often the only mutations over a long stretch of flanking sequences, suggesting that they may have resulted from the transposition activity of atons. recent expansion of aton-i and aton-ii families the large copy number of an aton family allows the estimation of its proliferation history. when a te family is amplified from a few original copies, the relative age of each element can be measured by the level of sequence divergence from the consensus sequence. the sequence divergence for each aton element from its corresponding family consensus sequences was calculated. the number of elements of a defined sequence divergence value was plotted against sequence divergence values to obtain relative proliferation time for each aton family. for aton-i, elements have identical sequences compared to the consensus sequence of the family and copies have divergence values between 0-1%. only copies exist at divergence values ranging from 2% up to 13%. in aton-ii, elements have sequences identical to the consensus sequence and copies have a divergence value ≤ 1%. forty seven copies exist at divergence values between 1% and 10%. three copies exist at divergence values from 10% to 20%. to understand the evolution of the aton families, phylogenetic analyses for the two aton families were performed. the elements in aton-i family can be grouped into five subclades. subclades “a” to “d” each contain a few elements. in contrast, subclade “e” is large and contains many identical element copies. similarly, the elements in aton-ii can be grouped into seven subclades. subclades “a” to “g” each contain a few elements that show minimal sequence similarities. subclade “i” contains copies that demonstrated some relatedness to other members in the clade whereas subclade “h" contained the largest number of elements with several identical copies. the phylogenetic analyses suggested that the high copy numbers for these elements are due to recent amplification of these elements a few ancient copies. distribution of aton-i in different strains of a. aegypti there can be differential accumulation of various te families in different strains or ecotypes of a species. for example, different lines of the same rice cultivar contain dramatically different copy numbers, from to ~ of the active mite family mping. since aton-i family appears to have amplified very recently in the sequenced liverpool strain of a. aegypti which was collected in from west africa, it is interesting to know whether this aton family is similarly distributed in a. aegypti mosquito strains collected from other locations. in the americas a. aegypti was first introduced from africa during european exploration and colonization in the late 1500 s thus providing at least four centuries of separation. the distribution of aton-i family in mosquito strains collected from different locations in the americas was surveyed. these strains included costa rica, ugal and rockefeller. khw is a white-eyed mutant line of rockefeller. the copy numbers of aton-i in these strains of a. aegypti were estimated using transposon display, a modified version of amplified fragment length polymorphism. when one selective base was used to reduce the total number of bands in each lane, approximately bands were visible on each lane of the liverpool strain, consistent with the expected results. despite the fact that the liverpool mosquitoes used in this study underwent at least consecutive generations of single pair inbreeding, there are still about bands have different sizes between the two individuals. a. aegypti has homomorphic sex chromosomes with a small sex-determining region on chromosome therefore the different sized bands are probably not caused solely by the different sex of the two individuals. the other strains showed a similar number of bands to that of the liverpool strain and a similar level of polymorphism between two individuals. these results suggest that aton-i has amplified to a similar copy number in all of the tested strains of a. aegypti. abundant new aton families in a. aegypti genome to find out how abundant atons are in the a. aegypti genome, exhaustive genome wide searches were performed to identify repetitive elements that contain terminal inverted repeats flanked by nucleotides “a” and “t”. the ress, when exist, were retrieved to confirm the target site dinucleotide “at”. a total of aton-like families were identified, including the two families described above. interestingly, all of these elements have the nucleotide “t” internal to the “a” on the 5’ end and an internal “a” to the “t” on the 3’ end. the additional aton families contributed to a total copies. sequences in each of the families are more diverged than those in aton-i and aton-ii. nevertheless, three families have an element with more than identical copies: aton-xii with out of aton-xxii with out of and xxiv with out of suggesting that these elements were recently or may be currently active. while each aton family has a unique internal sequence and tir sequence, weak sequence similarity exists at the extreme ends of tirs among some families. based on this sequence similarity, five groups that account for atons families can be identified. the length of the similar regions ranges from to nucleotides. to investigate whether atons are transcribed, ests containing aton sequences were retrieved from databases. of the families, resulted in est hits. the number of hit sequences varied from to among the families. since atons do not encode proteins, their presence on ests in the untranslated regions resulted from the cotranscription with the genes they are located in. comparison of atons with helitrons and mites atons lack protein coding capacity and their transposition is dependent on transposases produced by autonomous elements. identification of putative transposase sources for atons will shed light on their transposition mechanisms and facilitate their classification. the autonomous elements of dna tes are typically expected to bear similar tirs, to the non-autonomous elements they mobilize. occasionally, these similarities can be very limited; consequently, identifying the autonomous partner is more challenging. extensive searches for large dna fragments flanked by similar terminal sequences to those of atons were performed. all of the genomic dna fragments from 500 bp to 200 kb flanked by the termini of atons were retrieved and analyzed. close inspection of these large fragments did not yield conclusive clues to the putative transposase for atons. since aton elements, like helitrons, are always flanked by an “a” on the 5’ end and a “t” on the 3’ end, it is possible that atons are related to helitron elements that insert between “a” and “t”. however, there are marked difference between atons and reported helitrons. the latter have conserved terminal motifs of 5’ “tc” and 3’ “ctrr”, although atons have a “t” internal to the 5’ “a”, only families have a “c” after it. helitrons were originally thought to bear subterminal palindromic sequences, a number of elements found in fungi, sea urchin, sea anemone and drosophila are often palindrome free. the presence of subterminal palindromic motifs on some atons resembles that on some helitrons. helitrons typically do not bear tirs. however, it has been reported that the maize genome contain heltir sequences that have terminal inverted repeats resembling helitron 3' termini. to see whether there are autonomous helitrons in a. aegyypti genome that bear the tir sequences of atons as a terminus, genomic sequences of 20 kb near aton tir sequences were analyzed, nevertheless no helitron protein domains were found in these sequences. on the other hand, atons share some similarity in features to mites, they are quite small and have tirs. as described in results, the dinucleotide “at” may also be considered as a specific tsd sequence. if atons are mites, it is possible that transposase coding autonomous elements bearing the tir sequences of atons may exist in the genome. in an effort to find such elements in the whole genome, genomic sequences flanked by aton tirs were analyzed. however, no conclusive evidence was found for the presence of such transposase coding elements. a similar scenario occurred for the identity of a recently discovered drosophila element named dine-i. however, dine-i elements do not bear tirs that are the critical features of mites. in addition, dine-i has a preferred insertion site of “tt” and partial rephel coding sequences were found to be associated with some elements. these feature led to the proposal that dine-i elements are helitrons. in contrast, atons bear tirs like mites and insert at “at” like typical helitrons. therefore, the classification of atons and components of their transposition machineries remain to be determined.  genome wide analyses of tes in a. aegypti identified candidate elements from familes for very recent or current transposition activity. among the best candidate tes are five aton families, novel elements bear terminal inverted repeats and insert specifically at dinucleotide “at”. in this study, previously unidentified te families with nearly copies were characterized. however, despite exhaustive search efforts in the genome sequences, the autonomous elements and the classification of atons remain mysterious. 
close inspection of the elements revealed that these two families were previously mis-categorized and, unlike other known tes, insert specifically at dinucleotide “at”. atons are novel mobile genetic elements bearing terminal inverted repeats and insert specifically at dinucleotide “at”. active transposons are considered valuable tools to propagate pathogen resistance transgenes among mosquitoes, rendering the whole population recalcitrant to diseases. therefore, endogenous active transposons in mosquito genomes are highly desirable. transposon display analysis of aton- family using different a. aegypti strains suggests that the elements are similarly abundant in the tested mosquito strains. starting with the transposable element database of the yellow fever mosquito aedes aegypti genome, detailed analyses of the members of each te family were performed to identify sequences with multiple identical copies, an indicator of their latest or current transposition activity. aton-i has a total copy number of among which three elements have more than identical copies. aton-ii has a total copy number of among which three elements have more than identical copies. five aton families contain elements existing at more than identical copies, suggesting very recent or current transposition activity. transposable elementshelitronterminal inverted repeats aedes aegyptiminiature inverted repeat transposable elements studies on genetic control of mosquito mediated disease transmission gained traction recently due to mosquito transgenesis technology. genome wide searches revealed additional aton families in a. aegypti genome with nearly copies in total. among a dozen of potentially active te families, two dna elements are short and nonautonomous.
 mosquitoes are important pathogen vectors affecting human and other animals. studies on genetic control of mosquito mediated disease transmission gained traction recently due to mosquito transgenesis technology. active transposons are considered valuable tools to propagate pathogen resistance transgenes among mosquitoes, rendering the whole population recalcitrant to diseases. a major hurdle in this approach is the inefficient remobilization activity after the integration of heterologous transposon vectors bearing transgenes into chromosomes. therefore, endogenous active transposons in mosquito genomes are highly desirable.  starting with the transposable element database of the yellow fever mosquito aedes aegypti genome, detailed analyses of the members of each te family were performed to identify sequences with multiple identical copies, an indicator of their latest or current transposition activity. among a dozen of potentially active te families, two dna elements are short and nonautonomous. close inspection of the elements revealed that these two families were previously mis-categorized and, unlike other known tes, insert specifically at dinucleotide “at”. these two families were therefore designated as aton-i and aton-ii. aton-i has a total copy number of among which three elements have more than identical copies. aton-ii has a total copy number of among which three elements have more than identical copies. genome wide searches revealed additional aton families in a. aegypti genome with nearly copies in total. transposon display analysis of aton- family using different a. aegypti strains suggests that the elements are similarly abundant in the tested mosquito strains.  atons are novel mobile genetic elements bearing terminal inverted repeats and insert specifically at dinucleotide “at”. five aton families contain elements existing at more than identical copies, suggesting very recent or current transposition activity. a total of new te families with nearly copies were identified in this study. transposable elementshelitronterminal inverted repeats aedes aegyptiminiature inverted repeat transposable elements 